LOCUS       DAOPKQ010000001        27831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_1816_length_27831_cov_5.816028, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000001 DAOPKQ010000000
VERSION     DAOPKQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 27831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27831
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1816_length_27831_cov_5.816028"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..2221)
                     /locus_tag="PK146_00005"
     CDS             complement(41..2221)
                     /locus_tag="PK146_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HPE90647.1"
                     /translation="MKDFEKMGSFYLGRQVDDTSGAMTGDLLLYDAKDLTTHGMIIGM
                     TGSGKTGLAISLIEEALIDNIPVIAIDPKGDIPNLLLSFPELSPEDFLPWVSHQEASR
                     KGLSPEQFAEKTATDWKKGLQEWGIDGPRIRKMKESVDMTIYTPGSSAGRMVSILHSF
                     DAPPEAIRADGDLFRERIQTTASSILALMAIDADPLTSREHILISRVLEEKWSRGESL
                     DIPSLIEMIRVPPFRSVGVLDLETFYPSGARMDLVLRLNHLVAAPSFAPWMEGDPLDA
                     GRFLYTADGRPRASIFTISHLSDRERMFFVSLLLNEILGWVRTQPGTSSLRAILYMDE
                     IFGFFPPVMNPPSKTVLLTLLKQARAYGLGVLLATQNPVDLDYKGISNTGTWFIGRLQ
                     TERDKSRILEGLKGVSEKGFPLPELDRMISGLGKRVFLLHNVHEDRPVLFQTRWAMSY
                     LAGPVTREQIKTLAGDSPVVQVPPKASAPVIPDGPAVQAQAPVAPSVIPSGIPSVFLP
                     APASVQAEYAPFLGAFARVHFSDAKRKFAETRNFAYAVEISEGPLPVDWENAFGIDVD
                     PAGLSQGIPSPVMSEVASPGLDPKAYVRWGKDFLKWLPQNAMMTLMTCPSLKEVARPG
                     ESPSDFAARIALMKRELRDAEVEKIRRKYASKLTTLQNRLMRAEQAIEREAEQAKHKK
                     METAISFGTAIASALLQTELQLHFLCTLKILNHSTVVFLSQHYL"
     gene            complement(2328..2588)
                     /locus_tag="PK146_00010"
     CDS             complement(2328..2588)
                     /locus_tag="PK146_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90648.1"
                     /translation="MSGETVELRGVTPSELTSFFLCEGFHEAMPMGTWERGSTKARVG
                     GETTISIGSLRIPSVTVAFSGPERELGSLLARFRSCFLRAGG"
     gene            complement(2688..2960)
                     /locus_tag="PK146_00015"
     CDS             complement(2688..2960)
                     /locus_tag="PK146_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HPE90649.1"
                     /translation="MIRVSFFGIVREIVGEKETQVEAEDSVADLLEALSGRYGIGFGE
                     KVLKTQERDLDLILILNGRHIEHIGGLSAPLKEGDHLAVFPLVGGG"
     gene            complement(2970..4766)
                     /locus_tag="PK146_00020"
     CDS             complement(2970..4766)
                     /locus_tag="PK146_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPE90650.1"
                     /translation="MEALRLLRVDLSAGTVKQEPLNPDWVRQFIGGRGYGTKLLYEEL
                     KPGTDPLSEGNKLIFANGPLTGSNVPTGGRYMVVTKSPLTGFIASSNSGGYWGAELAR
                     TGYMALVVEGKSKKPVYLWIKDDKAELRDASSLWGKDTHATTDAVLKEVGDPKARVAC
                     IGPAGEKLSRIACIINDKHRAAGRSGVGAVMGSKNLKAIAVKGSGTFKAANPEGLKET
                     LRVGMQKIKENGVTGEGLPTYGTAVLVNILNQLGSYPYKNFQESQHEGAEKHSGEKLA
                     ETFLTGRTNCFACPIGCGRETALEDKKGEGPEYETLWALGSNCGVDDLEPIIRANYIC
                     NELGLDTISAGGTISAAMELYEKGFIPKEDLGVGPELKFGSADALVYWTEKMGKVEGL
                     GEKMAQGSYRLGELYGHPEFSITVKKQELPAYDPRGIQGIGLTYAVSNRGACHVRGYL
                     ISPEVLGIPEKLDPWVTEGKAQWGKIFHDLTAAIDSSGMCLFTSFALGAGDYADMLKH
                     GAGMDLDADSLLEAGDRIWTLERLFNLREGLDPARDDTLPVRLLEEPIPKGPAEGKKS
                     RIDVMIPEYYKLRGWDEKGVPKPSKLAELGIK"
     gene            complement(4912..6015)
                     /locus_tag="PK146_00025"
     CDS             complement(4912..6015)
                     /locus_tag="PK146_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE90651.1"
                     /translation="MTTDSVVLDLDESRIVIPRDRRLRKVYLEFSSRCNLNCPMCFRE
                     SWTEPQGDMDWDLFLRLASDLEQFPRLETVHFLGIGEPFCNPRLVEAVGIIKSGNRRV
                     RITTNATLLDRSAARALVAAGADIVDLSVEAENVPWPGRVNSLETLSKAAEELLEARR
                     HAGSNACETRIALVVTRSNLPLVPDIVRSLKGRGVSAVSVSNLLPTDMEADANRLYPL
                     SLEEEVAVRSKLYTQFQHAGIKPELPRFALSTERHCNFVRSESCFIRWDGLVAPCLRL
                     CHGYSEIVLGREKEVVPWFFGDLKRQGLAEIWDDPGYVSFRWRLRRALYASCLDCNLL
                     RSCEYAKTTESDCWGDSPACSDCLWDRNLIRCP"
     gene            complement(6139..6741)
                     /locus_tag="PK146_00030"
     CDS             complement(6139..6741)
                     /locus_tag="PK146_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPE90652.1"
                     /translation="MDEIDFVTLIPYVFITTFTPGPNNISCAASAARWGMKRTWGYLL
                     GIGSGFFFILLLSGAFSGTLIGFIPWLERYMRWVGAVYILWLAWGLAKSEGQGGIGAE
                     SPMRGFAKGFVLQFVNPKAILYAVTLYTAFLGPILARPLPVVFSAALFATIGFSSILA
                     WAVFGLGIDRFLQNPLHNRIVNVCFSLLLVWTAASVVGLL"
     gene            complement(7050..7370)
                     /locus_tag="PK146_00035"
     CDS             complement(7050..7370)
                     /locus_tag="PK146_00035"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE90653.1"
                     /translation="MEYTDTNSLVPKEVVKGVHGKFVHSPHMTMAWWHIEEGAMLPEH
                     SHEHEQVVNVIEGTLEISSGGKKWTLGPGSVLAIPSGVPHLARGVTECRVIDAFYPAR
                     EDYK"
     gene            complement(7387..8979)
                     /locus_tag="PK146_00040"
     CDS             complement(7387..8979)
                     /locus_tag="PK146_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE90654.1"
                     /translation="MDGARLLTGGRIKTMDPGNPTADSILLCGQRIAAVGTGKQVMGH
                     PLAFKADKIDLEGRRVIPGIIDGHLHFAAWSEGKMAVDLSGCRSIHEVVEKLKERAAS
                     LPDGRWIRGMHFDHSRFAENRLPDRSDLDAVKNPVLLTRVCYHAHCANSRALELAGMR
                     DRKDLPGGVQRDGAGNLTGVVLESGADLLYDAFRRDTPVGEEHLEGMAASMREMASYG
                     VTSISTSAAVHIGIDESFGPYQELRRRGRLLQRATIHVNDLLPFGISSFLGDEMIRYG
                     GLKLFADGGFCARTAAMSFEYRGMPGYRGELNYDPVLFREIVSRAQEQGVQVAVHTNG
                     DRAMDMVLDAMERAAELFPRPWLRHKLLHCYVVRPEQAARMAALGVVADVQPVFVSDE
                     IDIAEEGVPEEMLPFSYAWKSLREAGIIVAASSDCPAASPNPWRGVDAAVNRVRVGDR
                     TPVGGWYPGQKMDLDEALEMFTSNASFSIGMERVLGSLEPGKFADLVVLEDDPWEMAD
                     GDLSGVRVFATFRGGEPIFGKI"
     gene            complement(9110..10117)
                     /gene="dctP"
                     /locus_tag="PK146_00045"
     CDS             complement(9110..10117)
                     /gene="dctP"
                     /locus_tag="PK146_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE90655.1"
                     /translation="MRKILALVLVAVLVLSLGAAATAAPKWTFKMGHPNPVDVPYDKT
                     AHKFADLVSEKTNGQVKINIFPNNQLGDWTETFELISRGVVEMGFQIANAQYDPKLNF
                     AYYMPYVVSDIEEAKKAYAPGGWAFGIIKDLWLEHGIQPLAVYPIGMAGVSLRSMPPA
                     PGDPNVPKNMKVRVMPLAACSMTYEALGYIATPIPYAEVYSGIQTGIVDGQQGGPPFQ
                     AWSFRDVNKVWIQYNDYFEQHWITINKGVWDKLPADVQAALQEAANEASAIRWDQVET
                     EDADYRKVLKEEFGWDIVMLTDEELKACADKVRREVWPKMKDLLGEDLYTEVRLNSMV
                     E"
     gene            complement(10273..11574)
                     /locus_tag="PK146_00050"
     CDS             complement(10273..11574)
                     /locus_tag="PK146_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE90656.1"
                     /translation="MIIAINLAILIGLIVVGLPVPFCFMTAAIYMGIVYDFSFSFLLP
                     SGYYALNSLTLLSVPFFIMAGALMSSSGIAERLTDFAQAFLGRMRGGMGAATIVACAI
                     FGAISGTCSSAVACIGGIMIPRLEKLGYPRYYSVAMVGCASVLGQLIPPSVPMILYAW
                     VTQQSVAACFLATVIPGILTTILMCIVNWFEVKRMGTVQVEPQIPLSQKVQVIGRATK
                     HGIWSLLMPVIILGGIYGGITTPTESAAIAVFYAIVVGFLIHKELTPRILGQALVNSS
                     TTTGVCVLMLFFVSILAKVYTMQRIPQQLAEFMMTISNNRIVLLLLVNAFLIIIGMMM
                     DDFSGTMLSAPLLLPLMVTIGVHPVHFAAIMGTNLGLGNVTPPCAPILFLAGRIGGAS
                     VDEYFRPALKFMIFAQVPVIILTTYIPGLSMWLPRLIMGVQ"
     gene            complement(11599..12174)
                     /locus_tag="PK146_00055"
     CDS             complement(11599..12174)
                     /locus_tag="PK146_00055"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE90657.1"
                     /translation="MLAIVNTKFWRILGILQKIIMIISSIAILALVLVQVLLRYVFVM
                     PLMGVEEVATMVGFWLYFMGASWGTAERSHIKADLMQAAIKDPRKLIWVKVLTSAACV
                     ILSAMMIYWGWGYTVWGYTRMQLSPTLMIPMIYSQVSIFIGAILMFFYFCVELVDYFL
                     QATGKIPLDTPDGSCQPVDAPAICPADQVGD"
     gene            complement(12242..13384)
                     /gene="alr"
                     /locus_tag="PK146_00060"
     CDS             complement(12242..13384)
                     /gene="alr"
                     /locus_tag="PK146_00060"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPE90658.1"
                     /translation="MGWRPTRMEVSLDNLRYNYRLIKEHVGGARVIAVVKANAYNLGI
                     IQASWALRGAGADFFAVATPDEAIELREGGITSPVLVLGTSPYSVAGEYVRLGIRAAV
                     TDPMMARALSTEAVRQGRKAFVHIKIDSGLGRIGFIPGHTLEAVEDIVSLPGIDAEGA
                     FTHFALADTGDMTYTREQYRRFLEALDQLSAKGIRFRMRHCCNSAATLETPEMALDAV
                     RPGHIIVGMYPSPEVKRSIIVKPCFEFKTAIGLIRELPAGTGVSYGLDYVTGQHERIA
                     QLPLGYADGYYRELWNKDVEVLVGGVRCPVVGRICMDQMMVNVTSVPQASVGDEVVLI
                     GRQGSEEITLDEIAGKMGTLACVPPTLISKRVPRVYIDRSSEELSR"
     gene            complement(13389..14831)
                     /gene="ortB"
                     /locus_tag="PK146_00065"
     CDS             complement(13389..14831)
                     /gene="ortB"
                     /locus_tag="PK146_00065"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HPE90659.1"
                     /translation="MSRADDYSSVMARRTEIMRCSVGIDYGSFSTGKLAFDYERMMAE
                     TGYPLDEVKRIQRESNVGETPLIELKNLTALVRKISEPGRGARIFVKDEANNPARSFK
                     DRRASLSVHNALMKGYPGVVAATSGNYGAAVASQAARKNLSSIIVQEVFDSRGFEQPE
                     IAEKGRACEAYGSEVLQLSVGPELFYVFLLVLEETGFFNASLYTPFSIAGIETLGTEI
                     VEQTRELTGRDPDAIISTHAGGGITTGTARGVLKAGSPGTRIIGASVNLQGLHMASDR
                     DFNRKSFTTGHTGFGFPFAVWPDRSDVPRNAARVLRYLDRYVTVSQGSVFYITEALAQ
                     LEGLERGPAGNTSLTAAFSLARTMGEDEVVVVNETEYTGAGKHPYAQIAFARKMGVEV
                     FRGDPSTEKPGENIVIPMKPGQVAASEVDLDRLRESYVKNACKILGEEREITPADLDF
                     ICDDARLDRGRALELLTENGVGLSLGRKEG"
     gene            complement(14818..15138)
                     /gene="ortA"
                     /locus_tag="PK146_00070"
     CDS             complement(14818..15138)
                     /gene="ortA"
                     /locus_tag="PK146_00070"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HPE90660.1"
                     /translation="MKAKKGDWVQVYRVVLEAGERAPQVPEDTAKVPLEMKVKGSLLE
                     DSAVPGDEVTVETAAGRTITGKLVAVEPPYDVSFGPPPPELRTVGRELRKILAGGGCR
                     HEQG"
     gene            15312..16313
                     /locus_tag="PK146_00075"
     CDS             15312..16313
                     /locus_tag="PK146_00075"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HPE90661.1"
                     /translation="MKRFARKLELAILPTPIQPSGRLSVLLGSDNVVLKRDDLTGMAL
                     SGNKVRKLEYCAADAVARGSDILITCGGPQSNHARATAAVAAKLGMGCHLVLNGTPEA
                     LPEGNLFLDLLFGARTTFVSLPDLRDQAKSMEEIADAYRTEGHRPYVIPLGASDALGS
                     LGYIDAIGEIADQVEKVGIIPDRIVFACGSGGTLSGIVAGCLVHGLNCRVTAISVAFP
                     SQWFDEKVDEVFSGLREKYIPELPDPRGRFEVIDSYIGEGYGKASPELINFISDAASL
                     EAVLLDPTYTGKALFGLMKEIENGGIDKDEKVLFIHTGGVFGLFPYRQALLDAFNKT"
     gene            16473..16832
                     /locus_tag="PK146_00080"
     CDS             16473..16832
                     /locus_tag="PK146_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HPE90662.1"
                     /translation="MEGGKLFYFGGYAKYPDDTSGHQMFGYFGIGLEVDPENWEIVDV
                     ASTFVSELGCSFFKSTLVGKRMNEGGIAEAIKEIENRYFAKGKKTIIAAMIDAEKNFQ
                     NYLQTRKSNVRPQDSGY"
     gene            complement(16904..17665)
                     /locus_tag="PK146_00085"
     CDS             complement(16904..17665)
                     /locus_tag="PK146_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPE90663.1"
                     /translation="MKREVNFSRMSWFDVQNAMKDGCDTVMIPVGSVEQHGPHLPLTT
                     DSMVVDWIANKAAEQMDGVLVAPIVYYGVSYNHIDFPGTGSVQIDTLKRYLIDVCISL
                     LGHGFEKLIILNGHGGNNATVAAAAHDIRLQTKKFVGVITWPSLVLEAEKTLESEIVW
                     HADEGETSTVLWIAPELVKMERALNVTPKPIPHYVFTHEALFSNPVDQGLPPTKVLSE
                     SGIVGDAKLATPEKGKLILEEAAKNLAKVITELRN"
     gene            complement(17666..18832)
                     /locus_tag="PK146_00090"
     CDS             complement(17666..18832)
                     /locus_tag="PK146_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE90664.1"
                     /translation="MASIESQFLRCGVLFDGNNVLKDTTIEISEGKIASIGKEPLDKK
                     GVWDLSDQTIIPGIIDCHDHLGLDPGDEREQSLAPTPWISLVGSANARKILGAGITTL
                     RDVGEKDFIDVYWKKAITEGLIKGPNMLISGQPVIKTGGHGWYLGREADGKDEIIKAV
                     REQVKQGVDFVKVMVGGGLTTLGSDPAFAEFSEEEVFSLVKEAHRCGRKVIAHAHGGV
                     AADIAIEAGVDSIEHGVFLSRGQMESMRDKGIFLVVTYGIIEQIVQGENIPEFTKSKA
                     KEYIKKYFEVISTAREVGTMLAAGGDGLHGRPDREMFALVSSGFSNRDAIRIMTSDSA
                     KLCGIDAIAGTIEKGKRADIVGLGSNPLEKISNIRDVQFVCRNGEIVYYKGWLV"
     gene            complement(18819..20330)
                     /locus_tag="PK146_00095"
     CDS             complement(18819..20330)
                     /locus_tag="PK146_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HPE90665.1"
                     /translation="MTYRFEFLLPFVLYLIVLVVIGFLAYKKTKTMEGFHLGNRQLSP
                     WVAGISILFSGSSGWIFLGCAGLGYSMGPSAWFMLISFMITMLIAYTLIGKRLRNYSG
                     LLGSITYPEFFVRRVRSNRNGIRLIASLAVFVFMTTYVASQYVAAVKALKPIFNMTDL
                     QVVLLTAVVVTLYCLIGGFMAVCWTDFVQGMVILGGSIIMCVFLVVEAGGFGEVFSKL
                     AAIDPKLLSAKWATWPLILMFWTAGLGGVGRPHDTIRFFALKDSHSARGTAMVGEISL
                     LFNYWTGYMIGYIGRVFFPALKDPETIFPTLLIEMFNPWFAGIMIAALMALIMSTIDS
                     QLLSAASTFTEDFYHTYVNKRASEQRLVWVSRVSVVAIGVFGAVMAMYMTETVNNLVL
                     FATAGLSSTFGPALVLSLYWKRLTEEGIIASMLTGLVVSVYWFLSPLKAITGLHESAV
                     GFFFAITVAVVVSLLTKPLPSELVESDFEIIRKDYSGEEMAKIDSLGESYGLN"
     gene            complement(20715..21515)
                     /locus_tag="PK146_00100"
     CDS             complement(20715..21515)
                     /locus_tag="PK146_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HPE90666.1"
                     /translation="MDILKRSISPLSEAAWNEIDEQAVKVLKSRLSGRKFVDVKGPFG
                     WDHAVVPAGRLDVSRGEGRDEVHWGVHIVQPLVETRVSFEMDVWELDNAARGAKDLEL
                     DPLIKAVEKIASFEENAIYNGFEPGCIEGLAQAGASQQISISTEHPRDIMAGLSEGLV
                     FFRKNSIEGPYTLVAGPQLWQIIDVFGDGYPLKKRVSSLFDGGMILAPELEGGFLVSA
                     RGGDFELTLGQDLSIGYECTSGSKVQLFIAESFTFRVLEPNAVVPLVL"
     gene            complement(21543..21905)
                     /locus_tag="PK146_00105"
     CDS             complement(21543..21905)
                     /locus_tag="PK146_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPE90667.1"
                     /translation="MSNYHEPVEELGAEDRDISRALNSLKEEIEAVDWYHQRAAATKD
                     SSIRDIVIHNRDEEIEHAAMMLEWLRRKMPAFDDALRTFLFTEAPITEVEEAAVAGEQ
                     VAGKTSSGSSLGIGSLKG"
     gene            22168..23286
                     /locus_tag="PK146_00110"
     CDS             22168..23286
                     /locus_tag="PK146_00110"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPE90668.1"
                     /translation="MNWKARMPAPVRNMEFAEYATSREERPVLVDCALGTNPLGSPDR
                     VRELIRTGRLPDPGGYPGDDLPFRKALSDSWKGVFSPDEVIIGTGSIGLIISLARTFC
                     TTGAAVLGVTPQFPDGPMHFQLAGASYRQISLTPPGYGLEVSRIAEAMIGDESIIYVD
                     RPHNPTGQVPGLDEMALLAEECRKHGSILIVDEAYGDFLPSGESALNLASPSMVVLRS
                     FSKGRGLAGIRTGYCVVRDEEARRFLRKVAPPFSVSAIAMDMATAALLDMDYARRSRE
                     MAREVKARVIDTIQRTGGVSVARTHPEVPILLASLVSGRENLYEVLMEQGIRTEPGTC
                     FTGLGPESVRLRIPAPEQLEIFGALWRKATEKGKVSRM"
     gene            23631..24776
                     /locus_tag="PK146_00115"
     CDS             23631..24776
                     /locus_tag="PK146_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90669.1"
                     /translation="MSNPKEMKQGFFSRMMASSSYKKYILPGLISQSVIIAGGYGTGR
                     ELVEYFVNFGSLGGILGMALVTTTLWSLVFAVSYEFARTFKVYDYRSFFKELLGPGWV
                     LYEVCYIVLLLIVLGVVGATSGSIFMQSFGLPPLVGAALFLAGVATLTFFGSYVIEIA
                     MSWWSYLLYAVFLVFLLVGISQVGDQIGASLATGEIKEGWALGGFKYAFYNLGTFPAI
                     LFALNYIESRKEAVISGIMTSIITILPGFFLYLVIMGFYPGILDVEVPAYTILGEIAP
                     WLLPVYMVVLFGTMIETGAGFIHAVNERINSWMVDHKGKGLTKANRGVLGGLMALVGL
                     GVASFGLIGLIAKGYGTISWGFFLLHGVALFTLGLYKISKKNAKIPA"
     gene            24807..26198
                     /locus_tag="PK146_00120"
     CDS             24807..26198
                     /locus_tag="PK146_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPE90670.1"
                     /translation="MDWRSLAGLTSKNIEKSCAVIESDERFIPGAVAIKYFPMAIERA
                     KGSLVWDADGNRYIDFLTSAAVYNAGHCHPVVVEAVREQVGKLQNYTVVYFYNDRQVR
                     LAELLTSTAPGDFPKKAAFGFSGSDGVDAAIRAARSFTGRRQILSFKESYHGMTTASL
                     SVTGIVGSDVKKSVCLDPNVVFAEYPDPYRNPWGIDGYADPEGLTREAMRSVDEMLDR
                     HRGDVAAVLIEPAQGDGGMIFPPREFMTRLRGVTEREGIVLIDEEVQTGMGRSGRWWA
                     IEHFDIVPDLIVSAKALGGGLPLSAIIGRADILDAVPVPLLAYTHAGHAVSCASAEAM
                     FRVIRDEDLAARAEEKGKIIAEWFREKAEQLPFIGDIRQKGLLMGVEIVSDRSNKTPD
                     KAMALKISWAAWEKGLILITFGKHGNVLRVAPPLNIPDELLQEALGIMNASLEDAASG
                     RVSEDILPYLKGW"
     gene            complement(26258..27052)
                     /locus_tag="PK146_00125"
     CDS             complement(26258..27052)
                     /locus_tag="PK146_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPE90671.1"
                     /translation="MAVIYGVFCGGIYLLQDKMIYQPWGEITATPSRVGLFFENVSFE
                     ASDGVPLHGWFIPAEKGYGKVLLFFHGNAGNISHRLESIRTFHDMGLDVLIFDYRGYG
                     KSGGKPSEEGLYRDGTAAMEFLLERGYFPDEIIMFGRSLGGAVAARVSMETTPLALIL
                     ESSFTSLVDMASMVARFFPVRQLLRSEYPTESILPYMSCPVLVVHSPDDEVIPFSQGL
                     KLYEAAPEPKVFLEIKGDHNGGFILSGETYRQGLKSFLSDIACIGR"
     gene            complement(27076..27696)
                     /locus_tag="PK146_00130"
     CDS             complement(27076..27696)
                     /locus_tag="PK146_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90672.1"
                     /translation="MDGRVRIRDSLAFGWRAFRRNTLFFMVLLLVMGIPLAILNVSLN
                     ELPLVSPNPSVLFLRGVTWVLRVLVALVLIVVSLSFCDRGSFEFVQLKGMKPLLIPYL
                     IGSLLLSVLVGVGMALFIVPGVYIAVRYQFMPYLIIDRGMKPLEAFKAAGELVRGSWM
                     NLFLFLLCIVGINFLGTIPLGLGLLITVPVTFMAHARVYRDFVPGP"
BASE COUNT         6073 a         8208 c         7500 g         6050 t
ORIGIN      
        1 tcttcttttt attttgggca aaaagagtta caaaatcagt ttagagataa tgttgcgaca
       61 aaaacacaac agttgaatga tttaaaatct tcaatgtaca caagaaatga agctgtaatt
      121 cagtttgaag caaagcactt gcaatggccg ttccaaagga tatggccgtc tccatcttct
      181 tgtgcttggc ctgctcggcc tccctctcga tggcctgttc ggccctcatg agccggttct
      241 ggagggtggt cagtttcgag gcgtacttgc ggcggatctt ctccacctcg gcatcacgca
      301 gttcccgctt catcagggcg atccgggctg cgaaatccga cggggattcg ccaggcctgg
      361 caacttcctt caaagagggg catgtcatca gggtcatcat ggcgttctgt ggaagccact
      421 tgaggaagtc tttcccccac ctgacatagg ccttcggatc gagacccggc gacgccacct
      481 cggacatgac cggcgaaggg ataccctggg atagaccggc agggtccacg tcaatgccga
      541 aggcgttctc ccagtccacg ggaagtggtc cctcgctgat ctcgacagca taggcaaagt
      601 tcctggtctc ggcgaacttc cttttggcat cgctaaaatg gaccctggcg aaagctccca
      661 ggaagggggc gtactcggcc tggacactcg ctggagcggg gaggaatacc gagggtattc
      721 ccgagggtat cacactcggc gctacaggag cctgggcctg gacggcggga ccgtcgggga
      781 tgactggggc actggctttg ggaggaacct ggacgacagg actgtctccg gcaagggtct
      841 ttatctgctc tcttgtcacc ggcccagcca ggtaggacat ggcccagcgg gtctgaaaca
      901 gtacgggcct gtcctcatgg acattgtgaa gcaaaaaaac ccgtttccca agtcctgata
      961 tcatcctgtc cagctccggg agagggaacc ccttttccga gacccccttg agcccttcga
     1021 ggatccgaga cttgtcccgt tcagtctgaa gcctgccgat gaaccaggtc ccagtattgg
     1081 atatcccctt gtagtccagg tccacagggt tctgggtggc caggaggacc cccaggccgt
     1141 atgccctggc ctgcttcagt agcgtaagga gtaccgtctt cgagggcggg ttcatcaccg
     1201 gcgggaagaa gccgaagatc tcgtccatgt atagtatggc cctgaggctg gaggtgcccg
     1261 gctgggtcct gacccagcca aggatctcgt tgagcagtag cgatacgaag aacatcctct
     1321 cccggtcgga gaggtggctt atggtgaata tggacgcacg gggcctcccg tctgccgtgt
     1381 agaggaatct tcctgcgtcc agcgggtccc cctccatcca gggagcgaag gacggcgcag
     1441 cgaccaggtg attcagcctg aggaccaggt ccatcctggc gccggagggg taaaaggtct
     1501 ccaggtccag cactcccacg gacctgaagg ggggaaccct gatcatctcg atgagggagg
     1561 gtatatccag cgactctccc cgggaccact tctcttccag gaccctggag atcaggatat
     1621 gttccctgct ggtcaaaggg tcggcgtcga tagccatgag ggccaggatg cttgatgccg
     1681 tggtctggat cctctcacgg aagaggtctc cgtcggccct tatggcttcc ggaggggcgt
     1741 cgaaggagtg gaggatgctc accattcttc cggccgaact ccccggggtg tagatcgtca
     1801 tatccacgga ctctttcatc ttgcggatcc tcggtccgtc tatgccccat tcctgcagcc
     1861 ccttcttcca gtccgtagcc gtcttctcgg caaactgctc aggggagagc cccttcctgg
     1921 aagcctcctg atggcttacc cagggcagga agtcctccgg tgacagttcg gggaacgaca
     1981 gaagcaggtt gggtatgtcc ccctttgggt caatggctat cacaggaatg ttatctatca
     2041 gagcctcctc gatcagcgag atcgccaacc ccgtcttgcc gctgccggtc atgccgatga
     2101 tcattccgtg agtcgtcagg tccttggcat cgtagagcag aagatcaccc gtcatggccc
     2161 ccgaggtgtc atctacctgt ctccccaggt aaaaggagcc catcttctcg aaatccttca
     2221 ctggacccac ctccttcaca agaagtataa accgttatcc cctaaaaaac ctcgaagtgg
     2281 tcccctacag ggtacctcct ctatccatga cctggccctg cctgaactta ccccccagca
     2341 cgaaggaaac aggatctgaa gcgggccaga agggaaccca gctccctttc gggaccggaa
     2401 aaggcaacgg taaccgaagg gatcctcagc gaaccgatac tgatcgtcgt ctcccctccg
     2461 acccgtgcct tcgtacttcc tctttcccag gtccccatag gcatcgcctc atggaagcct
     2521 tcgcacagga agaacgaggt cagttccgat ggggtcactc ccctcaactc tacggtctcc
     2581 ccggacatat accccacccc tgtaaagtcc tcactccctt gaaagaaagg ggggctcttc
     2641 cgcattatcg cggaagagcc cctccgaaag aagtgctctc gcaggaccta gccaccgccg
     2701 acaaggggga agacggccag gtggtcccct tctttcaatg gagctgacag tccgccgata
     2761 tgttcgatgt gcctcccgtt gaggatcagg ataaggtcca ggtcacgctc ctgcgtcttc
     2821 aggaccttct ccccgaaacc tataccgtac cttccggaga gtgcctccag gaggtctgcc
     2881 acagaatctt ctgcttcgac ctgcgtttcc ttctccccga ctatctctct gacgatcccg
     2941 aagaaggaga cccggatcat atccgcctcc tatttgatgc ccagttcggc aagctttgag
     3001 ggcttgggaa cgcccttctc gtcccagccc cgaagcttgt agtactcagg gatcatcacg
     3061 tcgatgcggc ttttcttgcc ctcggcaggc cctttgggta tgggttcctc gagaagcctt
     3121 acgggaagag tgtcgtccct ggccggatcg agtccctccc tgaggttgaa gagccgctcc
     3181 agggtccata tccggtcccc tgcctcgaga aggctgtcgg cgtcaaggtc cataccggcc
     3241 ccgtgcttga gcatgtcggc atagtcgcct gcaccgagcg cgaaagaagt gaagagacac
     3301 atgcccgaag aatctatagc ggccgtcagg tcgtggaata tcttgcccca ctgggccttg
     3361 ccctccgtca cccaggggtc gagcttttcg ggtatgccca ggacctcggg agagatcagg
     3421 taccccctca cgtggcaggc cccccgattg ctcaccgcat aggtgagacc gataccctgg
     3481 atgccccgcg ggtcgtaggc ggggagctcc tgtttcttga cagtaatgga gaactccggg
     3541 tgcccgtaaa gttcccccag cctgtaactt ccctgtgcca tcttctcacc cagaccctcg
     3601 accttgccca tcttctccgt ccagtagacc agggcgtcgg cagagccgaa cttcagttct
     3661 ggccctacgc caagatcctc cttggggatg aatcccttct cgtagagttc catcgccgcc
     3721 gagatggtgc ctccggcact gatggtgtcc agtcccagtt cgttgcatat gtagttggca
     3781 cggataatgg gctcgaggtc gtcgacaccg cagttgctcc ccagcgccca tagggtctcg
     3841 tactcggggc cttcaccctt cttgtcctca agggccgtct ccctgccgca tccgatcggg
     3901 caggcaaagc agttggtcct ccctgtgagg aaggtctctg caagtttttc gccagagtgt
     3961 ttctctgcgc cctcatgctg cgattcctgg aagttcttgt aggggtagct cccgagctgg
     4021 ttgaggatgt tgaccaggac tgcggtaccg taggttggaa gcccctcgcc ggtaaccccg
     4081 ttctccttta tcttctgcat gcccaccctc agggtctcct tgagcccttc ggggtttgcg
     4141 gccttgaagg tcccgcttcc cttcacggcg atggccttca ggttctttga tcccataacg
     4201 gcaccaacgc cgctccggcc cgccgcgcgg tgcttgtcgt tgatgatgca ggcgatgcgg
     4261 gagagttttt ccccggcggg gccgatgcac gccaccctgg ccttgggatc tcccacttcc
     4321 ttcagtaccg catcggtggt cgcatgggta tccttccccc agagcgaaga agcgtctcgc
     4381 agctcagcct tgtcatcctt gatccacagg tagacgggct tcttcgactt cccctcgacg
     4441 acgagcgcca tatagcctgt cctggccagt tcggcccccc agtaacctcc ggagttgctt
     4501 gaggcgatga atcctgtcag ggggctcttg gtgaccacca tgtaccttcc gcccgtgggg
     4561 acgttggaac ccgtcagggg cccgttggcg aagataagct tgttcccctc tgagagaggg
     4621 tccgtcccgg gtttcagctc ctcgtagagc agcttggtcc cgtatccacg tccgcctatg
     4681 aactgtctca cccaatcggg gttcaaaggc tcctgtttga ccgttcccgc ggaaaggtca
     4741 accctcaaaa gacgcagcgc ttccatgtct ttccctcctc ttctcctttt gtgacatcct
     4801 tgccgcctca cgatgaagat ctctcgagac tctctatcct tcgttcaaag agttagcctg
     4861 aatacctcaa aggacctctc tgtcacctcc tttttcacca gggaatattt tctaggggca
     4921 ccgtataagg ttccggtccc acagacagtc gctgcaggcc ggtgagtcgc cccagcagtc
     4981 gctctcggtg gtcttggcgt actcgcagga cctgaggagg ttgcagtcca ggcaggaggc
     5041 gtagagggct ctcctcagtc tccacctgaa ggaaacgtaa ccggggtcgt cccagatctc
     5101 tgccagcccc tgtcttttca gatctccgaa gaaccagggg accacctcct tctccctccc
     5161 aaggacgatc tcgctgtagc cgtgacacaa cctcaggcac ggcgccacaa ggccatccca
     5221 tcttatgaaa catgactcag acctcacaaa gttgcagtgc cgctcggtag agagcgcgaa
     5281 ccttggaagc tcgggcttga tcccggcgtg ctggaattgg gtgtagagtt tcgaacggac
     5341 ggctacctcc tcttcgagcg acagcgggta gagccggttg gcgtcggcct ccatatcggt
     5401 cgggagcagg ttgctcaccg agaccgcgct gacacccctg cctttaaggg atcgcacgat
     5461 atcagggacc agaggcaggt tcgaacgggt aactaccagg gcgatacggg tctcacaggc
     5521 gttggacccc gcatgcctcc tcgcctccag tagctcctca gctgcttttg acagggtctc
     5581 cagactgttg accctccccg gccatggaac attctcggcc tcaacggaga ggtcgacgat
     5641 atcggctcct gcggcgacaa gagccctcgc ggcgcttctg tcgagcagag tggcgttggt
     5701 ggttattctt actcgcctgt tgccggactt gatgataccg acagcctcaa caagcctggg
     5761 attgcagaag ggttctccta ttccgagaaa gtggaccgtc tcaaggcggg ggaattgctc
     5821 caggtccgag gcaagccgca ggaacaggtc ccagtccata tcgccctggg gctccgtcca
     5881 ggattcgcgg aagcacatgg gacagttgag attgcatctg gaactgaact caaggtagac
     5941 cttgcggagc cgtcggtccc tgggaatgac gatccggctt tcgtcaagat caagaacaac
     6001 gctatcagtt gtcatggacc ccccctggga gctgtcggtc agacagcccg ctatccctga
     6061 caatatctat tatattgtca ggttaaagcg gtccctcgtc aagggcagac aagctgcaaa
     6121 caggctgaga gcgggttact acaggaggcc aaccacgctg gcggcagtcc atacaagaag
     6181 aagggagaag cagacgttga cgatccggtt gtgaaggggg ttctgcagga aacggtctat
     6241 ccccagccca aagaccgccc aggccaggat agaagagaaa ccgatagtgg caaagagcgc
     6301 agcggaaaac acgaccggga gggggcgagc caggatgggt cccagaaatg cagtgtaaag
     6361 ggtcacggcg tagagaatgg ccttggggtt gacgaactgc aggacgaacc ccttggcgaa
     6421 acctctcatg gggctttcag ccccgatccc accctgcccc tcgctctttg cgagtcccca
     6481 ggcgagccag aggatataga cagcgcctac ccatctcata tatctctcca accaggggat
     6541 gaaacctatc agtgtccccg agaaggcccc ggagagcaga agaatgaaaa agaaacccga
     6601 acctatgccg aggaggtaac cccaggtcct tttcataccc catcttgcag cgctggctgc
     6661 gcagcttata ttgttggggc ccggtgtaaa ggttgttatg aagacatagg gtataagggt
     6721 gacaaagtcg atctcgtcca agaatatctc cccttgcgct tccgagtcac ggccttgcca
     6781 ttgcctgtca tctcgagacc agagacacaa gcttcaacgt taaacggtga acggaaagac
     6841 cggaagcaga tcttttttcc cagggctaac ctgtttactg tttaccgttc actgttcacg
     6901 cctttgggtt tttcttgtta cgagtcacaa ggtgccccaa gcgcagcgac agggcgcgcc
     6961 cctcgttaaa actcctccct cttttaagca cctcgggacc tcgtcgggac caggtccttc
     7021 agcttgtctg gagggtacga gtcacggaat tacttgtaat cctccctggc cgggtagaaa
     7081 gcgtctatca cgcggcattc cgtaacaccc cgggcaagat ggggaacacc cgaaggaatg
     7141 gcaaggacag acccgggtcc cagggtccac ttcttgcctc ccgaggatat ctcgagtgtt
     7201 ccctcgatca cgttgaccac ctgctcatgc tcgtgggagt gctccgggag catcgctccc
     7261 tcctctatat gccaccaggc catcgtcatg tggggggagt ggacgaactt tccgtggacg
     7321 cccttcacga cctcttttgg gaccagactg ttcgtgtctg tatattccat ggcgaaacct
     7381 ccctctttaa atttttccga aaatgggctc accacccctg aaggtggcaa aaacccttac
     7441 gcccgacaga tccccgtcag ccatctccca gggatcgtcc tcgaggacca cgagatcggc
     7501 gaacttgccc ggctccaggg aacccaggac tctctccata cctatggaga aggaggcgtt
     7561 gctggtaaac atctcaagcg cctcgtcgag gtccatcttc tgacctgggt accagccgcc
     7621 gacgggtgtc ctgtcgccga ccctcaccct gttcaccgcc gcatctaccc cacgccaagg
     7681 gttgggggaa gcagccgggc agtcggaact ggcggcaaca atgataccgg cttccctgag
     7741 tgacttccac gcgtaggaga agggcagcat ctcctcgggg acaccctcct cggcgatgtc
     7801 gatctcgtcg gagacgaaga cgggctgaac gtcggcgacc acaccaaggg cggccatcct
     7861 tgcggcctgc tccggtctga ccacgtagca gtgcaggagt ttgtgcctaa gccacggcct
     7921 cgggaaaagc tcggcggccc tctccatggc atcaaggacc atatccatcg cccggtcccc
     7981 gttggtgtgg accgccacct ggaccccctg ctcctgggcc cttgacacta tctcccggaa
     8041 aaggaccgga tcgtaattca gttctcccct gtagcccggc atgcccctgt attcgaagga
     8101 catggcagcc gtcctggcgc agaaacctcc gtcggcaaag agcttaagtc cgccgtacct
     8161 tatcatttcg tctccaagga aggacgagat cccgaagggc aggagatcat tgacgtgtat
     8221 ggtcgctctc tggaggagcc tgccccggcg tcggagttcc tggtaggggc cgaaggactc
     8281 gtcgataccg atatgcaccg ccgccgaggt gctgatggag gtcactccgt aggaggccat
     8341 ctccctcatg ctggcggcca ttccttccag gtgctcctcc ccgacgggag tatccctcct
     8401 gaaggcatcg tagagcaggt cagcgccgct ttcaagcact acacctgtca ggttcccggc
     8461 gccatccctc tggacccccc cggggaggtc cttcctgtcc ctcatgcccg ccagctcaag
     8521 ggccctggag tttgcgcagt gagcatggta gcagaccctg gtcagaagaa cggggttctt
     8581 cacggcatcc aggtcggatc tatcggggag cctgttctcg gcgaaccggg agtggtcgaa
     8641 atgcatcccc ctgatccacc taccgtcggg taaagaggcg gccctctcct tcagtttttc
     8701 caccacctcg tggatcgaac ggcatcccga caggtcaacg gccatcttgc cctccgacca
     8761 ggcagcgaag tgaaggtgcc cgtcgatgat cccgggaata acccttcgcc cctccaggtc
     8821 gatcttgtct gccttaaagg caaggggatg acccatcacc tgtttcccag ttcccacggc
     8881 agcgatcctc tgtccgcaga gaaggatcga atccgccgtc gggttcccgg ggtccatggt
     8941 cttgatcctg ccgcccgtta aaagccttgc cccgtccata tcattaccac ctcgccgttc
     9001 atatcgtaac tgttccgttt tgcccgttct ttcaagcgag gggcgggagt tacctcccgc
     9061 ccctcgcctt tatagcttga gaactgttgt ccctgaaaaa agcgggaagc tattcgacca
     9121 tcgagttgag gcggacctcg gtgtaaagat cttcgccgag gaggtccttc attttcggcc
     9181 atacttccct gcggaccttg tcggcgcagg ccttgagctc ctcatcggtc agcatcacga
     9241 tatcccagcc gaactcttcc ttgaggacct tcctgtaatc ggcgtcctct gtctctacct
     9301 ggtcccaacg gatggcagaa gcctcgttgg ctgcctcctg caaggctgcc tggacatcgg
     9361 ccgggagctt gtcccatacg cccttgttaa tggtgatcca gtgctgttca aagtagtcgt
     9421 tgtactggat ccagaccttg ttgacgtcgc ggaaggacca cgcctggaag ggaggtccac
     9481 cctgctggcc atcgactata ccggtctgaa ttccgctgta gacctcggcg taggggatcg
     9541 gggtcgcgat gtagccaaga gcctcgtagg tcatcgagca ggctgccagg ggcatgaccc
     9601 ggaccttcat gttcttgggg acgttgggat cgccaggagc gggcggcatt gatcttagag
     9661 agacgccagc catcccgatg gggtataccg ccaggggctg gatgccgtgc tcaagccaga
     9721 gatccttgat gatcccgaag gcccagccgc ctggggcgta ggctttcttg gcctcttcga
     9781 tatcgctgac aacgtagggc atgtagtagg cgaagttcag cttcggatcg tactgggcgt
     9841 ttgcgatctg gaagcccatc tcgacaaccc cgcgggagat gagttcaaag gtctccgtcc
     9901 agtcgccaag ctggttgttg gggaagatgt tgatcttgac ctgaccgttg gtcttctcgg
     9961 agaccaggtc ggcgaacttg tgagctgtct tgtcatacgg cacatccacg ggattcgggt
    10021 gacccatctt gaaggtccac ttcggggccg ctgtcgcggc agctcccagc gagaggacca
    10081 ggaccgccac cagaacaagc gccaggatct tcctcatcta aaacacctcc ttgaatagta
    10141 cagaaaaagg gtttttcaag tccttctctg ctttgctaac ctcctatcct agaactcctt
    10201 caagcgggat acatattctt tcttgatcat gccacccccc ttcaggattg ctggatgata
    10261 aagagaattc ccctactgga cccccattat cagacgcggg agccacatcg aaagacccgg
    10321 gatgtaggtg gtcagaatga tcaccggcac ctgggcgaag atcatgaact tgagggcagg
    10381 cctgaagtac tcgtctactg acgcccctcc tatcctgccc gccaggaaca gtatcggagc
    10441 acagggggga gtgacgttgc ccagtcccag gttggttccc atgatggccg cgaagtgaac
    10501 cgggtgcact ccgatggtca ccatcagcgg aagaagcaat ggagcagaga gcatggtgcc
    10561 gctgaagtcg tccatcatca ttcctatgat tatcaggaag gcattcacca gcaggagcag
    10621 gacgatcctg ttgtttgaga tggtcatcat gaactccgcg agctgctgcg ggatgcgctg
    10681 catggtgtag accttggcca ggatggagac gaaaaagagc atcaaaacac agacgcccgt
    10741 ggttgtagag gagttgacca gggcctgtcc aaggatcctt ggggtcagct ccttgtggat
    10801 caggaaaccc accacgattg cgtagaagac tgcgatggcc gccgattccg tgggggttgt
    10861 tatccccccg tagatcccgc caaggatgat gacgggcata agaaggctcc agatgccgtg
    10921 tttcgtggcc cttcctatca cctggacctt ctgggagagg ggtatctggg gctcaacctg
    10981 aacggtcccc atcctcttga cttcgaacca gttgacaata cacatcagaa tggtggtaag
    11041 tatccccggg ataaccgttg ccaggaaaca tgccgcaacg gattgctgcg taacccaggc
    11101 atagaggatc atcgggacgc tcggcggtat cagctggcca agcaccgagg cgcaccccac
    11161 catggccacg gagtagtacc ttgggtatcc gagcttctca aggcggggga tcatgatgcc
    11221 gccaatgcag gcaacggccg aggagcaggt ccccgaaatg gccccgaaga tggcgcaggc
    11281 cactatggtg gcagcgccca taccgcccct catcctgccc aggaaggcct gggcgaagtc
    11341 ggtgagacgc tcggctatac ccgacgagga catgagagcc ccggccatga tgaagaatgg
    11401 gaccgaaagg agcgtcaggg agttaagggc gtaatagccg ctcgggagaa ggaatgagaa
    11461 gctgaagtcg tagactatgc ccatgtaaat ggccgccgtc atgaaacaga acgggacggg
    11521 cagccccaca acgattagtc cgataaggat ggcaagattg atcgcaataa tcattttcga
    11581 ccactcctac aactctttct agtccccgac ctgatcggca gggcagatag ccggggcgtc
    11641 cacaggctga caggatccgt cgggagtatc gagagggatc ttgcccgtag cctgaaggaa
    11701 ataatcgaca agttcaacgc agaaatagaa gaacatcagg atagcaccaa taaagatgct
    11761 cacctgggag tagatcatgg ggatcatgag agtgggggag agctgcatcc tcgtgtagcc
    11821 ccacactgtg tagccccaac cccagtagat catcatcgcc gagaggatca cacaggccgc
    11881 agaggtcagg accttcaccc atatcagttt cctgggatcc tttatggcag cctgcataag
    11941 gtccgccttg atatgggacc tctctgcggt cccccaggag gcccccatga aatagagcca
    12001 gaaacccacc atggtggcga cctcttcgac tcccatcagg ggcatgacga agacatacct
    12061 caggaggacc tggacaagga ccagggcaag gatagctatc gaggagatga tcatgatgat
    12121 cttctgcagg atgccaagga tcctccagaa cttcgtgttc acaatagcga gcattcattc
    12181 gacctccctt ttccttacgc tttaaagaag aaaacaaatg aaatgatacc acaaattaaa
    12241 ttcaacgact cagctcctct gaactccggt caatatagac ccggggcact cttttgctga
    12301 tcagcgtggg cgggacgcag gccagggtcc ccatctttcc ggcgatttcg tcaagggtga
    12361 tctcctcact cccctgtctg cctatcagga ccacttcgtc acccaccgag gcctggggga
    12421 cggaagttac attcaccatc atctggtcca tgcagatacg ccctaccact ggacaccgga
    12481 cacctccgac gagaacctcc acatccttgt tccagagctc cctgtagtaa ccgtcggcgt
    12541 aacccagggg aagctgggcg atgcgttcgt gctggccagt aacatagtcc agaccgtagc
    12601 tgacccccgt cccggcgggc agttcccgga tcaggccgat ggctgtcttg aactcgaagc
    12661 agggttttac gatgatcgag cgtttgactt cgggcgatgg gtacatgccc acaatgatgt
    12721 gcccaggcct tacggcgtcc agagccatct cgggcgtctc cagagtggcc gcagagttgc
    12781 agcagtgccg catcctgaac cttatcccct tcgccgagag ctgatccagg gcctccagga
    12841 agcgacggta ctgttcgcgg gtgtacgtca tgtctcccgt atcggcaagg gcaaagtggg
    12901 tgaaggctcc ttccgcatca atgcccggaa gggagacgat atcttccacg gcttccaggg
    12961 tatggccggg gatgaaaccg atacgtccca gaccgctgtc tatcttgatg tgcacaaagg
    13021 ccttcctgcc ttgtctgaca gcctccgtcg agagcgccct ggccatcatc gggtccgtga
    13081 cggccgcacg gatccccagg cgaacgtact ctcccgccac ggagtagggt gaggtaccca
    13141 ggacaaggac agggctggtt attcccccct ctctcaactc gatggcctcg tcgggggtgg
    13201 caacggcaaa aaagtccgct cccgcccccc tgagggccca ggatgcctga atgatcccca
    13261 ggttgtaggc gttggccttc accacggcga tcaccctggc gcctccgacg tgctctttta
    13321 tgagcctgta gttgtatcgc aggttgtcga gactaacttc cattctcgtg gggcgccatc
    13381 ccatccgttc atccttcctt tcttcctaat gaaagaccga ccccgttctc cgtgaggagc
    13441 tccagtgccc ttcccctgtc aagcctggcg tcgtcgcaga tgaaatccag atcggcggga
    13501 gtgatctctc tctcctcacc gagaatcttg caggcgttct tcacgtagga ctcccggagc
    13561 ctgtccaggt ctacctcgct ggcagctacc tgcccgggct tcatcgggat cacgatgttc
    13621 tctccgggct tctccgttga aggatctccc ctgaaaacct ctactcccat cttcctggcg
    13681 aaggctatct gggcgtaggg gtgtttcccg gcgccggtgt actccgtctc gttgacgacc
    13741 accacctcgt cctcgcccat agtgcgggcc agagagaatg ccgcggtaag ggaggtgttc
    13801 cctgcgggcc ctctctccag accttccagc tgggccagag cttcggtgat gtagaagacc
    13861 gacccctgac ttacagttac gtaccggtcc aggtagcgca gcactcttgc ggcgttccgg
    13921 gggacatccg agcgatcggg ccagactgca aaggggaaac cgaaccccgt atgccctgtt
    13981 gtgaaggact tacggttgaa atccctgtct gatgccatgt ggagaccctg aaggttgacg
    14041 cttgccccta tgatccttgt cccagggctt ccagccttca gaactcccct ggccgtcccg
    14101 gtagtgatgc ctcccccggc gtgagtgctt atgatcgcgt cggggtcccg tcccgtcagt
    14161 tccctggtct gctctactat ctcggtgcca agggtctcga tgccggctat tgagaagggt
    14221 gtataaaggg atgcgttgaa aaaccctgtc tcctcaagaa cgaggagaaa gacgtaaaag
    14281 agctcgggcc ccacagaaag ctggagcacc tcggagccgt aggcctcgca ggcccgtccc
    14341 ttctcggcta tctcgggctg ttcgaagccc ctgctgtcga acacttcctg gacgattatg
    14401 ctggagaggt tcttcctggc ggcctggctg gccacggctg ccccgtagtt gccgctggtg
    14461 gcggccacga caccagggta gcctttcatg agggcgttat gaactgaaag ggaggccctt
    14521 ctgtccttga aagacctggc gggattgttg gcctcgtcct ttacgaagat cctggccccc
    14581 cgtcccggct ccgatatctt ccttacaagc gcggtgaggt tcttgagctc aatgagggga
    14641 gtttcaccta cgtttgactc cctctggatc cttttgacct catccagcgg ataccccgtc
    14701 tccgccatca ttcgctcata gtcaaaggca agcttgccgg ttgagaagga gccgtagtcg
    14761 atgccgacag agcacctcat gatctcggtc cttcgggcca tcactgagga gtagtcatca
    14821 gccctgctca tgacggcacc ctcctccggc aaggatcttc ctgagctcac ggcccacggt
    14881 cctcagctcc ggcggtggag gcccgaagct gacatcgtag ggtggctcca cggctacaag
    14941 cttccctgtt atcgttcttc ctgcggccgt ctccacggtc acttcgtccc cagggaccgc
    15001 cgaatcctcc agcaggcttc cctttacctt catctccagc ggcaccttcg ccgtatcctc
    15061 cggcacctgg ggtgcccttt ctcccgcttc gagaacaacc ctgtagacct ggacccaatc
    15121 tccctttttt gccttcaata gttgccactc tcccgttgtt taagtgtatc tgcctgatac
    15181 caaaaaaccg cgcagggcct gggaaacagg gtctgcgaat ttacttaata tgcattattt
    15241 actttgttgt gtcaaaatag atatgctgtt cacaaacaag aggtacagac acaaaggagg
    15301 tcaaggagac attgaaaaga tttgcccgga aacttgaact ggcgatcctt ccgacaccga
    15361 tccagccgtc aggcagactc tctgtcctcc tggggagcga caatgtggtc ctgaaaaggg
    15421 acgaccttac gggcatggcc ctttcaggaa acaaggtaag aaaactcgag tactgcgctg
    15481 ccgatgccgt agccaggggg tcggacatcc tcatcacctg cggaggcccc cagtcgaatc
    15541 acgccagggc gacagctgcc gtggcggcca agctgggaat ggggtgtcac ctggtcctga
    15601 acggtacccc ggaggctctt ccggagggca acctctttct ggatctcctc ttcggcgcaa
    15661 ggaccacctt cgtctccctc cccgacctga gggaccaggc aaagagcatg gaagagatag
    15721 ccgatgccta ccgcaccgaa gggcaccggc cctacgtcat ccctcttggc gcttcagacg
    15781 ccctggggtc gctggggtac atcgacgcca tcggagagat cgcagaccag gtcgagaagg
    15841 tggggataat tcctgaccga atagttttcg catgcggctc aggcggaacc ctatcgggca
    15901 ttgtcgccgg ctgccttgta cacggtctca actgtcgagt gacggccatc agcgttgcct
    15961 tcccgtcaca gtggttcgac gagaaggtcg acgaggtctt ttccgggctc agggagaagt
    16021 acatccccga actgcccgat cccaggggaa ggttcgaggt catcgacagt tacatcgggg
    16081 agggttacgg gaaggcctct ccggaactga tcaacttcat cagcgatgcc gcatccctgg
    16141 aggccgtact cctggacccc acatataccg gcaaggctct cttcggtttg atgaaagaga
    16201 tagagaatgg cggcatcgac aaggacgaaa aggtcctctt catccacacc ggcggcgtct
    16261 tcggcctgtt cccatacagg caagcgctat tggacgcatt caataaaacc tagccaaaat
    16321 gaagggccag aatcactctt ctctcgagaa agttaggtac cgacaacacg ctcagaccag
    16381 atccttcttg cacgatagag atggagatcg atcaggaagg gggaacataa ttcttcccct
    16441 tatctaattc atttcgagga gggagcagtt tcatggaagg aggaaaactc ttctatttcg
    16501 gaggttatgc gaaataccct gacgatacca gcggacacca gatgttcggt tacttcggaa
    16561 taggtctgga agttgatccc gaaaactggg agatcgtcga tgtagcctcg acctttgtct
    16621 cagaattggg ctgctctttt ttcaagtcga ccctggttgg gaaaaggatg aatgagggag
    16681 gtatcgctga agctatcaag gagatcgaga acagatattt cgcaaaaggg aaaaagacga
    16741 tcatcgcagc gatgatagac gcagaaaaga atttccagaa ttacctccag accaggaaga
    16801 gcaatgtccg ccctcaagac tcgggatact aaattcccca aggaaaggtt tcagcagggg
    16861 agacgatcgc cctggaccgt ctcccctgct gatgggagga ccttcagttc ctcagttcgg
    16921 tgatcacttt cgcaaggttc tttgcagcct cttcaaggat taatttgccc ttttccggag
    16981 tcgcaagttt tgcgtctccc acgatacccg attcggaaag aactttcgtc gggggcaatc
    17041 cctgatcaac gggattgctg aaaagcgcct cgtgggtaaa gacataatgg ggtataggtt
    17101 tcggggtaac gtttaaagcc ctctccatct ttaccagttc cggggcgatc cagagaactg
    17161 tcgacgtctc cccttcatca gcatgccaca ctatctcgct ttccagggtc ttctcggctt
    17221 caagtaccaa tgagggccag gtaataacac caacaaactt ctttgtctga aggcggatgt
    17281 catgggccgc cgctgcgacg gtcgcattat tccccccgtg accgttaaga atgataagct
    17341 tctcaaaacc atgccccaaa agggaaatac aaacgtcgat caaatacctc ttcaaggtat
    17401 ctatctgaac cgaaccagta ccagggaaat caatgtggtt ataactgacc ccgtagtaca
    17461 ctatcggagc gacaagaact ccatccatct gctcagctgc tttgttcgcg atccaatcaa
    17521 caaccatgga atccgtcgtc aatggcagat gggggccgtg ctgttccacc gatcccacag
    17581 ggatcatcac agtatcacag ccatccttca tggcgttttg aacatcgaac caactcatcc
    17641 tgctgaagtt aacttctctt ttcattcaaa ccaaccatcc tttgtaatag actatttctc
    17701 catttcggca aacaaactgg acatccctga tgtttgatat tttttccaga gggttgcttc
    17761 ccaagccaac gatatcagcc cttttcccct tttcgatcgt cccggcaatt gcatctatcc
    17821 cgcagagttt ggctgaatcg cttgtcatta tcctgatggc atccctgttg gagaaacctg
    17881 aagacaccag ggcgaacatt tccctgtcag gtctcccatg aagcccgtca ccgcctgccg
    17941 ccagcattgt ccctacttca cgtgcggtgg aaatgacctc gaaatatttc ttgatatatt
    18001 cttttgcctt ggacttagtg aattcaggaa tgttctctcc ctgaactatc tgttcgatga
    18061 tcccataggt aactacaagg aagatccctt tgtcccgcat gctttccatc tgtccgcggc
    18121 ttaaaaaaac accatgttcg atcgaatcaa cacccgcttc aatggcaata tcagcggcaa
    18181 cgcctccatg cgcgtgggca atgacctttc ttccacaacg atgtgcttcc ttgacaagag
    18241 aaaagacctc ttcctcggag aactcggcaa aagcaggatc agatcccagg gtggtcaatc
    18301 ctcctccaac cattaccttt acaaaatcaa ctccctgttt cacctgttcc cttaccgcct
    18361 tgattatttc gtctttccca tcggcttccc ttccgagata ccacccatga ccaccggttt
    18421 tgattaccgg ttgtcccgag atcagcatat tcgggccctt tattaaccct tcggttattg
    18481 ctttcttcca gtagacatcg ataaagtctt tctcgccaac atccctgagg gtcgtgatcc
    18541 cggccccaag gatcttccgt gcattggcag aaccgacaag acttatccag ggagtgggtg
    18601 caagagattg ttctctttca tctcctgggt ccaaaccgag atggtcgtgg caatctatga
    18661 tcccgggaat gatcgtctgg tcgctcagat cccagacacc ttttttatcc aggggttctt
    18721 tgcctatgga cgctattttc ccctccgaga tctcgatagt ggtgtccttt aaaacattgt
    18781 tcccgtcaaa aaggacacca catctcaaga actggctttc aattgaggcc ataactctcc
    18841 ccaagggaat cgatctttgc catttcctct cctgaatagt cctttcggat gatctcgaaa
    18901 tcgctttcta caagctcgga aggcaaaggc tttgtaagaa ggctcacaac aaccgctacc
    18961 gtaattgcga aaaagaaacc aaccgcactt tcgtggagtc cggtaatggc ctttagagga
    19021 gagaggaacc agtaaacgct gaccacgaga cctgtaagca ttgaggctat gatcccctct
    19081 tccgtcaagc gtttccaata gagagacaga accaacgcgg gcccaaaagt cgaggataaa
    19141 ccagcggttg cgaagagcac caggttatta acggtttctg tcatgtacat cgccatgact
    19201 gccccaaaga ctcctatcgc gacaactgaa accctcgata cccacaccag acgttgttcc
    19261 gaggcccttt tgttaacata ggtatgataa aaatcttccg taaaggtcga cgccgcactg
    19321 agtaactggg aatctatggt tgacatgatc aatgccatta aagccgcgat cattattccc
    19381 gcaaaccagg ggttgaacat ctcgatcaac agtgtcggga aaatggtctc gggatctttc
    19441 agggcaggga agaaaaccct tccgatataa ccgatcatgt aacctgtcca atagttgaat
    19501 agaagcgaga tctctccaac cattgcagtt cctcttgcgg aatgggaatc cttcaatgca
    19561 aagaaccgaa tggtatcgtg aggacgtcct acccctccga gccctgccgt ccagaacatg
    19621 agtatcagtg gccaggttgc ccacttcgct gacaatagtt tgggatcgat agccgccagt
    19681 tttgaaaaga cctctccaaa gcccccggct tcaaccacca ggaacacgca cattattatg
    19741 gagccgccca ggatgaccat tccttgaaca aagtcagtcc aacagactgc catgaaccca
    19801 ccgatgagac agtaaagggt tacaaccacc gccgtaagaa gaacgacctg caggtccgtc
    19861 atgttgaata tcggttttaa tgcctttact gccgcaacat attgggaggc cacgtaagtt
    19921 gtcataaaaa cgaacacggc caaggaagct atcagtctga tcccgttcct gttggacctg
    19981 acccttctta caaagaattc agggtaggta atgcttccga gcaagccaga gtaattgcgt
    20041 aatcttttcc caatgagggt atatgcgatc agcatggtta tcatgaagct tataagcata
    20101 aaccatgctg atggccccat actgtaaccc agtccggcac atcccaggaa gatccaacca
    20161 cttgaaccgg aaaagagaat acttattcct gccacccagg gactcagctg acggttccca
    20221 agatggaacc cttccatagt ctttgtcttc ttataagcca gaaaaccgat aacaacaaga
    20281 acgatcagat aaagaacaaa gggaagtaaa aattcgaatc gataagtcat tccgtcttca
    20341 cacccttttc ttcccagcaa aataatagag cactgtgaca ctggctatta ggaccggaac
    20401 gatgacaacc aggaaccaag ctgaaaagtc catctcctaa cctccttttg cgatcgtcag
    20461 tactgtcggg atctgaagac cagaatccaa taaaaaacgc gcccagaggc aagcctctgg
    20521 gcgcgtaccc ttcgcctgaa gaaagaggac gtctttcctc aaagggacac cagcaggttc
    20581 attcacattc ttgcgatagc atacacatca cgacctgaaa ctgtcaacca ggagacaaag
    20641 atcttatttt aacgatcatc tccgagatct gatcagaatt gcgttccttc gacccttcca
    20701 ctctcctgaa aaattcagag caccagcgga accactgcgt tgggctctaa caccctgaag
    20761 gtaaacgact cggctatgaa gagctggacc ttgctcccgg aggtacactc gtagcctatg
    20821 gagaggtcct gccccagggt gagctcgaag tcgccacccc tggcagacac gagaaaaccc
    20881 ccctccagtt cgggtgcaag gatcatcccg ccgtcgaaca gggaggagac cctctttttc
    20941 aggggatatc catcaccgaa gacatcgatg atctgccaga gctggggccc tgcgaccagg
    21001 gtataggggc cctcgatgga gttcttgcgg aaaaatacaa gcccctcgga aagaccggcc
    21061 atgatatccc tggggtgttc ggtggagatg gagatctgct gggacgcccc tgcctgagcc
    21121 agcccttcaa tgcagcccgg ttcaaatccg ttgtatattg cattctcctc gaaggaagct
    21181 atcttctcga cagccttgat gagagggtcc agttccagat ccttggcacc cctggcagcg
    21241 ttgtcgagtt cccagacgtc catttcgaag gagacgcgag tctccaccag gggctggaca
    21301 atgtgcaccc cccagtggac ttcgtccctg ccttcacccc tggagacgtc gagtctgccc
    21361 gccgggacga ctgcgtggtc ccatccgaat gggcctttca cgtcaacgaa ctttcttccc
    21421 gaaagcctgc tcttcaggac ctttacagcc tgttcgtcaa tttcattcca ggctgcttcg
    21481 ctaagcggag atatcgaacg cttcagaatg tccattctct tttccccctt ccctctcgat
    21541 ccttaacctt tgaggcttcc aattccaagg ctgctacctg acgatgtctt ccccgctacc
    21601 tgttctcccg cgactgccgc ttcctccacc tcggttatgg gagcttcggt gaagaggaag
    21661 gtcctcagcg catcatcaaa agcgggcatc tttcttctca gccactcgag catcatggcg
    21721 gcgtgctcga tctcctcatc cctgttgtgg atcactatgt ccctgatgga actgtccttg
    21781 gtagcggcgg cacgctgatg gtaccagtcc acggcctcga tctcctcctt gaggctgtta
    21841 agagccctgc tgatatccct gtcctctgct ccaagttcct cgaccggttc gtggtagttg
    21901 gacatgctca agtccctcct tcaggatcta tggtttctgg cggtccttat ccggacccac
    21961 gtctttcgct ggataacatg gtatcatcac ctctccgcgg ggacaaccgg ggggttacgt
    22021 ttctctttgt gccctctctg cggtgcaaat gttccggttt ggtcttgact ttaccgttca
    22081 ctattcaccg tttacgcctt tcgccactca ccgttttcct gcttcctgct atcattgcct
    22141 ccatatccgc aaatggaggg tgagatcatg aactggaaag cgaggatgcc cgcccctgtg
    22201 aggaacatgg agttcgccga gtacgccact tccagggagg aaagaccggt actggtcgac
    22261 tgcgccctcg gcaccaaccc acttggttcg ccagaccgcg tcagggaact aataaggaca
    22321 gggaggttgc cggacccggg cggatatccc ggtgatgacc tcccattccg gaaagccctg
    22381 tcggactcct ggaagggggt cttctcaccc gacgaggtca taatcggcac cggctccatc
    22441 ggcctgataa taagtctcgc cagaaccttt tgcaccactg gagcagctgt tctcggcgtt
    22501 accccccagt tccccgacgg gccgatgcac ttccagcttg ccggggcatc ctacaggcag
    22561 atatccctga ccccaccagg gtacggactc gaagtttcgc gaattgctga agccatgatc
    22621 ggtgatgagt cgatcattta cgtcgacagg ccccacaacc ccacggggca ggtccccggc
    22681 ctggacgaga tggcccttct tgcggaagag tgccgtaagc atggttccat cctgatcgtt
    22741 gacgaggcct acggtgattt cctcccctcc ggcgagtcgg ccctgaacct ggcctcgccc
    22801 tcgatggtgg tcctcaggtc cttctccaag gggcgaggtc tggccgggat acgtaccggc
    22861 tactgcgtag ttcgtgacga agaggccaga aggttcctgc gaaaagtcgc accacccttt
    22921 tcggtcagtg ccattgccat ggacatggcc actgcagccc ttctcgacat ggattatgca
    22981 cggcgttcaa gagaaatggc tcgtgaggtc aaggcccggg tcatcgatac tatccaaagg
    23041 acgggagggg tatctgtggc aaggacccac cccgaagtgc cgatcctcct ggcatcactg
    23101 gtctcgggcc gggaaaacct ctatgaggtc ctcatggaac agggaataag gaccgaaccc
    23161 ggaacctgct ttactggcct gggaccagag agcgtccgtc tcagaatacc cgccccggaa
    23221 cagctggaga tcttcggggc actctggaga aaggccacag aaaaaggaaa agtttcacga
    23281 atgtgaaatt tgttgacaag agggagatgc tgatatagac tccccgaaac gctttgtttt
    23341 ttcataatgc atatcttgtg aaggagggtg ccctgtgcgg atagagagag cttttcgtgg
    23401 tcgctttata tacggcatta ttctctatgt tgccttgggg tccccctcct ggcgcggttg
    23461 agccatactt ttccgcgata aaccgggaga aaggggcccc taaaggggcc cctttcttgt
    23521 ttgaggaggt gttgtccggg aatctggata agtaccaagc cgagaaagag aaacaggatc
    23581 gccccttgat gacgagggtc agagaaaaag acagagaggt gttatacgag atgagcaatc
    23641 caaaagagat gaaacagggt ttcttcagca gaatgatggc ttcttcttcc tacaagaaat
    23701 atatcctccc aggccttatc tcccagtcgg ttattatcgc cgggggctac ggcactggcc
    23761 gtgagctggt cgagtacttc gtcaacttcg ggagcctcgg cgggatactg ggaatggcgc
    23821 tggtgacaac aaccctgtgg tcccttgtct ttgccgtcag ctacgagttc gcgcgcacat
    23881 tcaaggtcta cgactaccgc agcttcttca aggaactcct tggaccgggg tgggtcctgt
    23941 acgaggtctg ctacatcgtc cttctcctta tcgttcttgg tgtcgtcggg gccacatcgg
    24001 gaagcatctt catgcagtcc ttcgggctcc ctccgctggt gggcgcggcg ctcttcctcg
    24061 cgggcgtagc cacgctgacc ttcttcggga gctacgtgat cgagatcgcc atgtcctggt
    24121 ggtcctacct tctctacgcc gtattccttg tattcctgct ggtcggcatc tcccaggttg
    24181 gagaccagat aggcgccagc ctcgccacag gagagatcaa ggaggggtgg gccctgggag
    24241 gcttcaagta cgccttctac aaccttggga ccttccccgc cattcttttc gctctcaatt
    24301 acatagagag caggaaggaa gccgtcatat cggggatcat gacatcgata atcacgatcc
    24361 ttcccggatt cttcctctac ctggtcataa tgggcttcta cccggggatc ctcgacgttg
    24421 aggtacctgc ctacacgatc ctcggagaga tcgcaccctg gctcctgcct gtctacatgg
    24481 tcgtcctgtt cggaacgatg atcgagacgg gtgcaggctt catccacgcc gtcaacgaga
    24541 ggatcaactc ctggatggtc gaccacaagg gcaaaggact gaccaaggcc aaccggggag
    24601 tccttggagg cctgatggcc cttgtcgggc tgggagtggc atccttcggt cttatcggcc
    24661 ttattgccaa agggtacggg accatcagct ggggtttctt cctgcttcac ggcgtcgccc
    24721 tcttcacgct gggcctgtac aagataagca agaagaacgc aaagatccct gcatgatatc
    24781 caggtgaaat gggaggctgc tttcatatgg actggagatc tctggccggg ttgaccagca
    24841 agaacatcga gaagagctgt gccgtcatcg agagtgacga aaggttcatt cctggcgccg
    24901 tcgcgatcaa atacttcccc atggctatcg agagagccaa gggatccctc gtatgggatg
    24961 ctgacggcaa caggtatatc gacttcctga ccagcgcggc cgtctataat gcaggccact
    25021 gccaccctgt ggtggtagag gccgtcaggg agcaggtggg gaagctccag aactacacgg
    25081 tggtctattt ttacaacgac agacaggtca ggcttgccga actgctgacc tccaccgcac
    25141 ccggggactt ccccaagaaa gccgccttcg gcttctccgg ctccgacggc gtggatgccg
    25201 ccataagggc agcccgctcc ttcactggca ggagacagat cctcagcttc aaggagtcct
    25261 atcacgggat gaccacggct tcgctctccg tgacgggcat agtcggcagc gatgtcaaga
    25321 aaagcgtgtg cctggaccct aacgtggtct tcgccgagta ccccgacccc taccgaaatc
    25381 cctggggcat agacggttac gccgaccccg aaggtttgac gcgggaggcg atgagatccg
    25441 tggacgagat gctcgacagg catcgagggg atgtggcggc cgtgctcatc gaaccagccc
    25501 agggcgacgg agggatgatc ttccccccca gggagttcat gacccgctta aggggtgtca
    25561 ccgaaaggga agggatagtc ctcatcgacg aagaggtcca gaccggtatg ggacgcagcg
    25621 gccgctggtg ggccatagaa cacttcgata tcgtccccga cctcatcgta tcggcaaaag
    25681 ccctgggagg aggcctcccc ctttcggcaa tcataggcag ggcagatatc ctcgacgccg
    25741 tgccggtccc cctgcttgcc tacactcacg cgggccacgc cgtcagctgt gcttcggcag
    25801 aggccatgtt cagggttatc agggatgagg atcttgcggc cagggctgaa gaaaagggaa
    25861 agatcattgc ggagtggttc agggaaaagg cggaacagct cccctttatc ggggacatcc
    25921 ggcagaaggg cctgcttatg ggcgtggaga tagtctccga caggagcaac aagacgcccg
    25981 acaaggcgat ggcgctgaag atatcctggg ctgcctggga gaaggggctt atcctgataa
    26041 ccttcgggaa acacgggaac gtgctgaggg tggctccacc gctcaacatt cccgatgagc
    26101 tcctccagga agctctcgga ataatgaatg cctccctcga ggatgcggca tcagggaggg
    26161 tgtccgagga catcctgccg tacctcaagg ggtggtagca acaaacccac ctccttcttc
    26221 tttgggagac ggcccggcaa atagtccggg ccgttttcta tcttcctatg caggcaatgt
    26281 ccgaaaggaa tgacttcagc ccctggcggt aggtctcccc tgagaggatg aagcctccgt
    26341 tgtggtcgcc cttgatctcc agaaagacct tgggttcagg ggcagcctcg taaagcttca
    26401 gaccctggga aaaggggatc acttcgtcat cggggctgtg gaccacgagt acgggacagg
    26461 acatgtaagg gaggatgctc tctgtcggat actcgctgcg cagaagctgg cgaaccggga
    26521 agaaacgggc gaccatggag gccatgtcga caagtgatgt aaaggacgat tccaggatca
    26581 gggccagggg ggtggtctcc atggagaccc ttgcagccac ggccccgccc agggatctcc
    26641 cgaacatgat tatttcgtcc gggaaatacc ctctctccaa caggaactcc atagccgccg
    26701 tgccgtcccg gtagagaccc tcttccgatg gcttgccccc gctctttccg tagccgcggt
    26761 aatcaaatat gagaacgtcc agccccatat cgtggaacgt cctgatcgac tccagcctgt
    26821 gggaaatgtt gccggcgttg ccgtggaaaa agagcaggac ctttccgtaa cccttctccg
    26881 ccgggatgaa ccagccatgg agaggcacgc cgtcggaggc ttcgaatgag acgttctcaa
    26941 agaaaagacc aaccctggag ggggttgctg tgatctcgcc ccagggctgg tagatcattt
    27001 tgtcttgcag caggtatatt cccccgcaga aaaccccgta gatgactgcc acccataaag
    27061 caaggcgggt gatgctcatg gtcccggaac gaagtccctg tagaccctgg catgggccat
    27121 gaatgtcacg gggactgtta tcaggagccc aaggcccaga gggatcgtcc ccaggaaatt
    27181 tatccccact atgcatagca ggaaaaggaa aagattcatc cacgagcccc ggaccagctc
    27241 cccggcagcc ttgaaagcct ccagtggttt catcccccgg tcgatgatca ggtagggcat
    27301 gaactggtac ctgacggcga tgtagacccc gggcacaatg aaaagtgcca ttcccacacc
    27361 gaccagcacc gaaaggagaa gggaaccgat aaggtaagga atgagcaaag gtttcatacc
    27421 ctttaattgc acaaactcaa aggaaccccg gtcacagaat gagaggctca cgacgataag
    27481 caccagggca acgagtactc tcaggaccca tgtcacaccc ctgaggaaca gcaccgaggg
    27541 gttcggcgat accaggggca actcgttgag ggatacgtta agaatggcca aaggaatacc
    27601 catgaccagc agaaggacca tgaagaaaag ggtgttccgc ctgaaggccc tccacccgaa
    27661 agccagtgag tcccttatcc gaaccctgcc gtccattctg tctccttccc gggcacctgg
    27721 gggccctgtg tcgctcgctg tcagaactta aacgtactcc ccggccttct ccaccccgtt
    27781 caacaccctt agagcgcctt ctgccagtgc cttcatctcg tcctctcccg g
//
LOCUS       DAOPKQ010000002        19818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_2895_length_19818_cov_4.236654, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000002 DAOPKQ010000000
VERSION     DAOPKQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 19818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19818
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2895_length_19818_cov_4.236654"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK146_00135"
     CDS             <1..651
                     /locus_tag="PK146_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HPE90673.1"
                     /translation="VGKGPLARNIRLQIPPFTLIGATTRLGLLTSPLRARFGIVEQLE
                     LYSPVDISQIVTRGSRLLSVDITSEAAMTIARSARGTPRVALRLLRRVRDVAEVRCAS
                     GIDKELARTALEMLGVDSEGMDERDRRYLRALVDLFGGGPVGLSTLSAALNEESQTIE
                     DIYEPFLIQKGFIERTPRGRRATVNGYSCLGRIPPRDRLSEQGLFTLLEEENGETD"
     gene            733..2070
                     /locus_tag="PK146_00140"
     CDS             733..2070
                     /locus_tag="PK146_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPE90674.1"
                     /translation="MLLQVTSLPGEANSALSIGLSTGDDRAVFSAPNSQINLASFSGG
                     KASFRSTVNVVAISPGVIKAGSVNLKLPVTVRSNVILRWNNRPYRGHYRLVQQGSGFN
                     VVNVVGIEDYLKGVLKMEVNPAWPMESLKAQAIIARTYALRNRGRHGSSGFDLCALSH
                     CQVYRGVNAEDQVLTKAVDQTRGQVLKFQGGLAATFYHADSGGHTAEVSSVWGSSVPY
                     LKGSPEPLKYQSPYSSWQTTLRLSEIQRVLSSKGIYVGRLTGISVSSTDSGGRAVDVT
                     LTGTAGRTTIRASQFRTIVGPDRIRSTFFTMGGGPVAAAPFIPNDTTSPGSGEDKVFP
                     LTPKDEQMLIAFTQQGVFKTEELMDMLVKPEKRGFYLRKALERQSGAGSLQTPPSFDA
                     PVTGDTVTFSGRGWGHGVGLSQWGAKSLAEAGWDHIRILEHYFPGTEISRISN"
     gene            2135..3169
                     /gene="queA"
                     /locus_tag="PK146_00145"
     CDS             2135..3169
                     /gene="queA"
                     /locus_tag="PK146_00145"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPE90675.1"
                     /translation="MFDINSYDYYLPESRIAQEPVEPRDSSKLLVMDRFSGTIRHRVF
                     NEIMSLLREKDLLVINDTRVVRARLRGVKAGEGASIEVFLLRPLGGKRCVLWEVLVRP
                     GRRVRPGQLLVLDGGIEVVIGERTSQGSRSCFFPEGTDVWRLMEETGEIPFPPYVHNR
                     SIDPERYQTVYGVNRGSVAAPTAGLHFTPGLLREIEAAGIRTSRITLNVGLGTFRPVK
                     EDDIRMHSMHPEECTIPEATAVAVRETKASGGRVVAVGTTVVRALESRAAEDGTVYPG
                     SFSTEAFFYPGYHFRTIDAMITNFHLPRSTLLMLVCAFAGNELTMKAYREAIETGYRF
                     FSFGDAMFIQ"
     gene            complement(3179..3622)
                     /locus_tag="PK146_00150"
     CDS             complement(3179..3622)
                     /locus_tag="PK146_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HPE90676.1"
                     /translation="MQKNDRPEDGTSSILEMLSEIERMVDGELFLPTLQEDWDRDPPK
                     EVSWAVANVLSLPLSQAARILLISSLLGSNGRDLTCRFNELALLSGLSERTAYRALKE
                     IKQSGLFKIQATPGSGVRISAEWIWDEPRDRAREFFQERMPGKAP"
     gene            3771..4565
                     /locus_tag="PK146_00155"
     CDS             3771..4565
                     /locus_tag="PK146_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HPE90677.1"
                     /translation="MWTMGFLLPHAPILVPEVAARSGSGTGKTLEGLRSLGGRLKELA
                     PDHLLVLDPHAVSDRTFTLINPGRFVGDLGNFGAPSVSISLKGAAEEGERLIEYISSF
                     IPSSACRPSEIDLDYAAVVSLHLLGPALWKTPSMILANPVTIDHCQAYELGRHLREFV
                     SGKKWGLLASGDLSHRLTRDAPSGFHPDGRVLDEAIVESLKRSSPDPVLDLPGQVIRN
                     AGECGLRSVLALIGLSMSSPIEVLSYESPFGVGYATALLDLSGEGE"
     gene            4565..5125
                     /gene="amrA"
                     /locus_tag="PK146_00160"
     CDS             4565..5125
                     /gene="amrA"
                     /locus_tag="PK146_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPE90678.1"
                     /translation="MDGLARSVKGEAHPYLDLARRAVRLTALGQDPFRHLDPREYNAT
                     TEMLIERGCFVSIRMASTGELRGCIGTIRPVRKSLWEEIVANAAAAASEDPRFFPLGP
                     DEAEECRVSVDVLDEPCPVGDLSELDPLLFGVIVEKEGRKGVLLPDLDGVDTVEQQLS
                     IAMRKAGIGSSDGVRIFRFTVRRFSE"
     gene            5150..6136
                     /gene="amrS"
                     /locus_tag="PK146_00165"
     CDS             5150..6136
                     /gene="amrS"
                     /locus_tag="PK146_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPE90679.1"
                     /translation="MDRTALYWIARGDDINCHLCPRECIISQGLTGACGVRENSGGRL
                     VSLNYGWACVAVDPVEKKPLYHWRPGTRILSLGTFGCNLFCPYCQNYHLSRSKNHKGL
                     SWTNPSDVLRLLQHHDAPSVAFTYNEPTVWYEFVRDTSLLLKREKKGVVLVTNGYISR
                     KPLEDLIHLVDAMNIDLKGFSDSTYSMLGGTLAPVLDTVRIAFERSVHVEITHLVVPG
                     INDSIEEFRHLVRWIAGISESIPLHISRYFPDYRWALPPTPIPSIRVREEIASEFLEN
                     VYSGNLQSRNNTLCPVCGEVLVERDDLYRASVHLLEDGTCPRCRTPRGIVMS"
     gene            6195..7727
                     /gene="hutH"
                     /locus_tag="PK146_00170"
     CDS             6195..7727
                     /gene="hutH"
                     /locus_tag="PK146_00170"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPE90680.1"
                     /translation="MGSSVLELNGHSLRLEDVVDVAREGRKIALDRSAVAFVERGSRM
                     VRSWVEDGRIIYGITTGFGDLASQVIPPDKSEKLQENLLTSHACGVGEPFPEEIVRAI
                     MLLRINSLIRGFSGISMETLSRLVDFLNIGLHPVIPCKGSVGASGDLCPLSHLAISLL
                     GLGEVFFQGKRMKASEALRSAGLEPIALGPKEGLALNNGTAAMTGVAVMAVWEAMAVL
                     KTADIVGAMSLEALHGVPFAFDHRTHELRPHQGQIKVAKNIRGLVSGSRILEKFGNER
                     VQDAYSLRCIPQVHGASRDGIQYVKNVIQIEINSVTDNPLIFPAEGEAISGGNFHGQP
                     VALAMDFFGMAMAEIGNISERRTARLVDGKLSGLPPFLVENSGINSGFMIPQYVAAAL
                     VSENKVLAHPSSVDSIPTSANQEDHVSMGMYSARKGKEILDNVKKILAIELLLAAQGL
                     DFSSPLEPGAGTGAAYRKLREEIPYLEKDVFLHPMIDRIIKGVEDRSYLKAVEEATGE
                     LE"
     gene            7764..9770
                     /locus_tag="PK146_00175"
     CDS             7764..9770
                     /locus_tag="PK146_00175"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPE90681.1"
                     /translation="MPDNRRIEAAMRISLGDELPSPPPFDPQIRRAPDRGYRLDKRQT
                     LTSLKNALRYIPEKLHQVLAPEFLDELRSRGRIYGYRYRPSTPPKGLPIDSYKGKCLE
                     GKAFQVMIDNNLDPDVALYPYELVTYGETGSVCQNWMQYRLIRKYLEELTEDMTLVVE
                     SGHPLGLFRSGPDAPRVIITNSMMVGMFDNQDDWEIAAQMGVANYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRKLGLSTGKGLEGVLFVSSGLGGMSGAQPKSAEIAGCVGIIAE
                     VDISRIETRHKQGWVSVVSSDLDEVFRIAGENLSSRKGISIAYHGNIVELLQYAVDRN
                     VKIDLLSDQTSCHAPYDGGYCPFQLSFDERTKMLRKDRKIFRQMVDESLSRHFDLIRT
                     IHERGTYFFDYGNSFMKAVFDAGRLDIAKNGKDTREGFIFPSYVEDIMGPELFDFGYG
                     PFRWVCLSGRPEDLEKTDQAAMECIDPERRPQDRDNYLWVRDAGRNRLVVGTQARILY
                     QDAEGRVRIALRFNEMVRHGEIGPVMLGRDHHDTGGTDSPFRETANIKDGSNVMADMA
                     IQCFAGNAARGMSLVALHNGGGVGIGKAVNGGFGLVLDGSERVDEVIRRSLSWDVIGG
                     VARRSWARNPNSIETIIGHNASSGDHVTLPFLADDRFLLSLIEE"
     gene            9804..10721
                     /gene="ftcD"
                     /locus_tag="PK146_00180"
     CDS             9804..10721
                     /gene="ftcD"
                     /locus_tag="PK146_00180"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPE90682.1"
                     /translation="MIMRIVECVPNFSEGRRKDVIESIVAPLRDRKGCQLLDHRSDEN
                     HNRLVVSLAGDPEAIMDSLIRSAEAAFSLIDMNVHSGAHPRIGALDVVPFTPLKGLSM
                     EECVQIAREFGRRLNEQFKVPVYYYEEAATRPDRKNLENIRKGQFEGLKKAIGDPERH
                     PDVGEAKMHPTAGATVVGARKFLVAFNVNLGTCRIEIAKEIARSIRFSGGGLAHVKAI
                     GLALEEKGMVQVSVNIVDHEKNPLYRVLELVRMEASRWGVPVVETEIYGMVPASAIFQ
                     SAAHYLQAAGLEPEQIIELKLLDMSGEQS"
     gene            10718..11965
                     /gene="hutI"
                     /locus_tag="PK146_00185"
     CDS             10718..11965
                     /gene="hutI"
                     /locus_tag="PK146_00185"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPE90683.1"
                     /translation="MTTKLFRDARIHTPVSDGGGCVSWERGALLVRDGLIERVGREDD
                     VVQGLDPLQIDQEIGLGGRCVVPGFVDPHTHMCFSELREKEFATRLSGVPYLEILRSG
                     GGILSSVRSVRSACDETILGRTLKNIHISLSHGTTTVEIKSGYGLDTASEIRLLRLIE
                     RVGRQTPVTVVPTFMGAHAVPSEFTDDPEGYVDLLVKEMIPAVSAECTPPFCDVFCEE
                     SVFSVPQTRRILDTAREHGMKLKVHADEVIDTGGAALAAEMGAVSAEHLLAAREENLA
                     KMASAGVIAVLLPATAYSLRKPYADARKMLDLGLTVALATDCNPGSSFTQSVPFVFGL
                     AVMNMGMTVDEALYGCTLNAAKAIGVDGSCGSLERNKLADLVVLDGDTPAILAYNCGV
                     APVLSVYKRGECVVQRTDDRRIN"
     gene            11965..12600
                     /locus_tag="PK146_00190"
     CDS             11965..12600
                     /locus_tag="PK146_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPE90684.1"
                     /translation="MKDIASLPVGDLIEELGSSSATPGGGSASALCASIAASLTAMVA
                     NLTVGRSRFSGVQEEMVQAAERGKALSREFLDLAMKDCDAFDRFMKALALPKDSEQER
                     ETRRISMQEALKGSTLVPMRMVELAGDVAENCLAVASMGNRNAITDAGVSALLIKAAS
                     RGAAMNARINLSSIEDGSFVEENLRRLEADLERTGTAADKVLAAVSSELPR"
     gene            12600..13652
                     /gene="mltG"
                     /locus_tag="PK146_00195"
     CDS             12600..13652
                     /gene="mltG"
                     /locus_tag="PK146_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPE90685.1"
                     /translation="MTKDTGPATVSWAGRVVHLILLLVTFASFIALAQYLPLKLAGTI
                     QMGRGDPMDVLVLPGMNAREISRVFGSSGLTDRPNELSRWFAREGIDRSIRPGLYRIR
                     KGSPWEIARQMAVAEPEVSGITLYPGETYEVLLARLGETFEGSLARDDLFPDTLRPLL
                     PSGPGSRIAFLIPETYSVTPSDGAVNELVRAASALWMKRVGERVLGPGVDADALLERA
                     IVASLVEREARHDHERPIISGVIRNRSEAGMKLQIDATVVYAWSLRGVELKRVLYRDL
                     EIDSPFNTYRYAGLPPAPICTPSEGSWLASIEPEDVPFLYYVAMPDGTHLFSRTFSEH
                     SEAVRRSRNAFGAVHQ"
     gene            13685..15040
                     /locus_tag="PK146_00200"
     CDS             13685..15040
                     /locus_tag="PK146_00200"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPE90686.1"
                     /translation="MDRLFDVTDAISIPGTSFGEVFIQRSFARHCLLDDGKFEEVSSS
                     LTEGTGTRIVVGDRTYFTHSSGSDLSSAAFTISDGKKRAGLSAGSPLPKGDKSLLPLP
                     EIEEEAPFERMRELDKMIRKLSPKVRQVSFAWSSGARETAIAGSLNPLCRLSVFSTRF
                     SATVVVESGGSLHSGRDVRAFSLPQEEFLRSNDLEDVAREAFRRAVLLSEAKPCPAGV
                     MPVVLAGAAGGTMIHEACGHSLEADIVLNDYSSFRDCLEKQVASPLVSLVDDPTLPGL
                     YGSYPFDGEGTLSRRTVLIRDGFLKSFLTDLLSSRQGGFPVTGNGRRSSFRHPPVPRM
                     SNTFIMNGDGDPEEIISGVDRGLLVLKMGGGEVNSTSGDFVFQVTEGYLVSKGKRSYP
                     VRNAMLTGNGPRVLMDIDAVGNDLHFDPGVCGKSGQQVPVTDGQPTIRIRQLVVGGSR
                     T"
     gene            15042..17258
                     /locus_tag="PK146_00205"
     CDS             15042..17258
                     /locus_tag="PK146_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPE90687.1"
                     /translation="MKVTRSKPRNKEKTGAREPVRAEGRRPPWFTMIVLVLLLVDLYL
                     LATLFLFRTGDLGRIVHFWTLEIFGGGVIVLLLFGAYSSISWLMGYQVPSPWRQALAS
                     LIMYLCCALLLGLSTLSGIEPVVGILRPGRPGYLLAGVLLRHIGPFGAVLAGFGGMAS
                     AMVLYGIINRRNVAAAASLLSGVLRSFCALLSPLSGGHPAGTAPSQEGTKKTPGKTAP
                     ETVTKTGRTSGSATPGKIDIPDTIVDETAVDKVQNLSDTIPGKTLSFPIPLDVFGEPD
                     DFSFEVDKGLLSRQGENIVDTLEEFGISAELAETVVGPTVIQYRIQLAPGIKVSRVSS
                     LANDLAVSLAVPSLRVEAPIPGKTYIGIEIPNPDRKPVNIRTVLEHSSFQDSDSDLAI
                     PVGVGVDGRHLTIGLEKMPHLLVAGTTGSGKSVFVSCCIAALACRMRPEELRFLLIDP
                     KRVEMKVFERLPHNITPPVVESRQAVHALGWAIREMEKRYEMFAQARVRNIKAFNARA
                     LPLDRLPYIIIVIDELADLMFTSPREVEDYICRLAQMARATGIHLVIATQRPSVNVVT
                     GLIKANIPARVAFTLPSQTDSRTIIDVSGAERLLGSGDMLFLTPRLPKPLRIQSPWMD
                     EPVIQRFMEYLSALFGDPVFLDIDMQEDDEQDGGRVHLDDPLLEEAMGLILQSGIASA
                     SRLQRQLRIGFTRAARLIDTLEQVGILGPQEGSRPRDILVDEEEARQILGASLNTP"
     gene            17273..18034
                     /locus_tag="PK146_00210"
     CDS             17273..18034
                     /locus_tag="PK146_00210"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPE90688.1"
                     /translation="MEAMVLPQINAHFEPAIEPPSTVIVAGGRAPGISWLREVCCSRD
                     VWAVDRGLDSCMSADIVPSLFIGDGDSVSRKAMRWSQLKRVTSILCPPEKNLTDLQLA
                     LKEAGGKGSSAIITGAFGGRFDHLFANVYSLIWAEEEWGPRVRCMADEKEVFFLIRGG
                     ESLSLSGLERGTVFSTLALSEECSGIDMKGAKWQVKGGRLLLREPFAISNILSGDAEG
                     ALFDRTPLRVSLSSGWLGVYFMTSLSAEQDQKGRF"
     gene            complement(18031..18225)
                     /gene="rpmB"
                     /locus_tag="PK146_00215"
     CDS             complement(18031..18225)
                     /gene="rpmB"
                     /locus_tag="PK146_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPE90689.1"
                     /translation="MANVCDCCGRGRVTGNSVSHSNRHTRRTWGINLQNVKVDVGCNE
                     SRRMKICTKCLRSGRVRRAV"
     gene            18365..18604
                     /locus_tag="PK146_00220"
     CDS             18365..18604
                     /locus_tag="PK146_00220"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPE90690.1"
                     /translation="MSFTKDMTDLQEIVSRLDSGKIPLEESLELFERGIGLVNSCRKY
                     LEEAQQKITLLTDDNGDHEGREWDPLSGDGPIDGN"
     gene            18594..19517
                     /locus_tag="PK146_00225"
     CDS             18594..19517
                     /locus_tag="PK146_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPE90691.1"
                     /translation="MEINFPPVKENPHDRISGCRFFVDKAIADLCKERPERIPERLWE
                     SMTYSLTAGGKRVRPVLCILAGEAFGASREDLIPMALACEMVHTASLIHDDLPAMDND
                     SMRRGKPTNHVIFGEPLALLAGDSLFLWAFEYARKGLLAGNAFPHISILEALGVLLDA
                     SGPAGICGGQVLDTDPVSAGPELEHPWTVARSKTAVLISASIQAGAILGGAEPREREA
                     FAEFGDHLGTAFQIVDDIIDVTGEAARIGKTPGKDRILGKVTFVSVFGLEKARRMARQ
                     ETFMALESLSTIKKDVADLERFPRILAERDS"
     gene            19546..>19818
                     /locus_tag="PK146_00230"
     CDS             19546..>19818
                     /locus_tag="PK146_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HPE90692.1"
                     /translation="MRELLDRIVDHRDLKELDYRQLDSLAEEIRETIVGTVQANGGHL
                     ASSLGAVELNIALLRAFDPSRDRIVFDVGHQAYAYKILTGRKDQFHT"
BASE COUNT         4433 a         5419 c         5762 g         4204 t
ORIGIN      
        1 gtagggaagg gacctctcgc aaggaacata agattgcaga taccaccttt cacccttata
       61 ggggcaacga cccgtctggg cctcctaacc tcgcccctgc gtgcaaggtt cgggatcgtg
      121 gagcagcttg aactctactc cccggtggat atttcgcaga tcgtgacccg cggctcgaga
      181 ctcctttcgg tggatattac ctctgaggcg gccatgacga tagccagaag cgccaggggg
      241 acacccaggg tcgccctgag acttttgaga agggtacggg acgtggccga ggtcagatgc
      301 gcttccggca tcgataaaga gctggccagg accgccctgg agatgctggg agtcgattcc
      361 gaggggatgg acgagcggga caggagatac ctcagggcct tggtcgacct cttcggaggt
      421 ggccccgtag gtctgtccac cctctccgcc gccctgaacg aagagagcca gacaattgag
      481 gatatttacg aaccctttct tatccagaag ggcttcattg agagaacgcc gagggggagg
      541 agggccactg tcaacggcta ctcctgcctg ggaaggatcc ctccaaggga ccggctgtcc
      601 gaacagggcc tttttaccct tctggaagag gagaacggag agaccgactg atacgaaaga
      661 tccttggaga acgggtgtgt gatcatgaaa ttattttttc aaaggacatt aatctcgttg
      721 gtattcgctg cagtgttact tcaagttaca tctcttcccg gagaggccaa ttccgcgctt
      781 tcgatcggcc tctctaccgg tgacgacaga gccgtctttt cagccccgaa cagccagata
      841 aacctggcca gtttctccgg cggaaaggcc tccttcaggt ccaccgtcaa cgtggtcgcc
      901 atttccccgg gggtcatcaa ggctggttcc gtgaacctca agcttcccgt tacggtaagg
      961 agcaacgtaa ttttaaggtg gaacaacagg ccctaccgcg gtcattacag gctggtccag
     1021 cagggatcag gttttaacgt tgtcaacgtg gtgggcatcg aggactatct caagggtgtc
     1081 ctgaagatgg aggtgaaccc cgcctggccc atggaatccc tgaaagccca ggctatcatc
     1141 gcaaggacct acgcactcag gaaccggggc agacacggaa gcagcggttt cgacctctgc
     1201 gccctcagtc actgccaggt ttacagggga gttaacgctg aggaccaggt ccttacgaaa
     1261 gcagtcgatc agaccagggg acaggtcctg aaattccagg gtggtctcgc cgccaccttc
     1321 tatcacgccg acagcggagg acacactgcc gaagtctcct ccgtctgggg gtctagcgta
     1381 ccctatctga aagggagccc cgaacccctg aaatatcaat ccccctactc aagctggcag
     1441 acgacgttga ggctttcgga gatacagagg gtcctttcct cgaaggggat ctacgtcgga
     1501 aggctcacgg gtatctctgt ctcatcaact gacagcggag gcagggccgt ggacgttacc
     1561 ctgacgggaa cagcgggcag gaccacgata agggcaagcc agttcaggac cattgttggc
     1621 cccgacagga taaggagcac cttcttcacc atggggggag gtccagttgc tgctgctcca
     1681 tttatcccca atgacaccac ctctccgggt tccggagagg ataaggtctt ccctctcacc
     1741 ccaaaagacg aacagatgct catagccttc acccagcagg gtgttttcaa gaccgaggaa
     1801 ctgatggaca tgctcgttaa gcccgaaaag agggggtttt acctgagaaa agctcttgag
     1861 agacagagcg gagcaggttc tcttcagacg cctccctcct ttgacgcccc cgtcacgggc
     1921 gatactgtca ctttttcagg ccggggctgg ggtcacggag tggggctttc gcaatggggc
     1981 gccaaaagtc ttgccgaagc cggatgggat cacataagga tactggaaca ctacttcccg
     2041 ggtacggaga tatccaggat ctccaactga catggaagga cctttcagaa cgtatctccc
     2101 agggagcgaa ggcgctctgg aaggatcggg atcgatgttc gatattaaca gctacgatta
     2161 ctatctgccg gagtcgagga tcgcccagga accggtcgaa cccagggact catcgaagct
     2221 gctggtcatg gaccgcttct cggggacgat ccgccacagg gttttcaatg agatcatgtc
     2281 acttctgcga gaaaaggacc tcctggtgat caacgacacc cgagtggtca gggcccgcct
     2341 ccggggtgtc aaggctggag aaggggccag catcgaggtt tttctgctga ggccccttgg
     2401 cgggaagaga tgcgtcctct gggaggtact ggtgcggccg ggcagacggg tcagacccgg
     2461 ccagcttctt gtcctggatg gagggatcga ggtagtgatc ggggagagga ccagccaggg
     2521 gtccaggagc tgcttcttcc cagaggggac cgatgtctgg aggctgatgg aggagaccgg
     2581 ggagatcccc ttccccccct atgtacataa ccgatccata gatcccgaga gataccagac
     2641 ggtctacgga gtgaacaggg gctccgtggc tgccccaacg gcgggccttc actttacacc
     2701 cgggctgctt cgggagattg aggcagcggg tatcagaacg tcacggataa ccctgaacgt
     2761 cggtctgggg accttcaggc ctgtgaagga agatgatatc cggatgcaca gtatgcaccc
     2821 cgaagagtgc accatccctg aagcgacggc cgtggccgtg agagagacca aagcgtccgg
     2881 aggaagggtg gtcgctgtgg gaaccaccgt ggtaagggca ctggagagca gggctgcaga
     2941 agatggcacc gtttacccag gttcgttctc gacagaggcc ttcttctacc ccgggtatca
     3001 tttccggacc atcgatgcca tgatcaccaa cttccatctt ccccgtagca cccttctcat
     3061 gctggtctgc gcctttgccg ggaacgaact gacgatgaaa gcctacaggg aggccatcga
     3121 aacgggatac cgtttcttct cctttgggga tgccatgttc attcagtgaa catatctttc
     3181 agggagcctt tccaggcatc ctttcctgga agaattccct ggccctgtca cgaggttcat
     3241 cccagatcca ctctgccgat atcctcacgc cactacccgg agtagcctgg atcttaaaca
     3301 gccctgactg ctttatttcc ttcagcgccc ggtatgctgt tctctctgag agtccagaga
     3361 gcaaggcaag ctcgttaaag cgacatgtca gatcgcggcc gttactcccc agaagggaag
     3421 atatcaggag gatcctcgcc gcctgtgata gtggaaggga gagcacattg gcgacggccc
     3481 aggagacttc cttcggggga tcccgatccc agtcctcctg cagggtcggc aaaaacagtt
     3541 caccatcgac catcctctcg atctcggaga gcatttccag tatggaggag gtcccatcct
     3601 ccggtcggtc attcttttgc acctgtccct ccagtcttcg cagggatgaa cccggttctt
     3661 tcccttctgg accccttctg aagaggatcg gggcccctgt tggaaaaaca ttctctgact
     3721 gtcataatac atgaaaaggg acttttacag aatctcgggg agggattgtc atgtggacga
     3781 tgggtttttt gcttcctcat gccccaatac tggtccctga ggtagcagca agatccggat
     3841 cagggaccgg aaagaccctg gaggggcttc gatccctcgg cggaagactg aaggaacttg
     3901 cccccgacca tcttcttgtc ctggatcctc acgctgtttc agacaggacc tttaccttga
     3961 taaaccccgg gaggttcgtg ggagacctgg gtaatttcgg ggccccttcg gtatcgatct
     4021 cgctcaaggg agcggcggag gagggagaaa ggctaattga atacatatcc tcttttatcc
     4081 cttcttcagc ctgcagaccc tccgagatcg acctggatta tgccgctgtc gtatcccttc
     4141 acctgcttgg tccagccctt tggaagaccc cctcaatgat cctggccaat ccggtaacaa
     4201 tagatcactg tcaggcatac gaactaggca gacacctcag ggagttcgtt tcggggaaaa
     4261 agtggggact tctggcaagc ggcgatctct cgcaccgttt gaccagggat gctccatcag
     4321 gtttccaccc cgacggcagg gtcctcgacg aagctattgt tgaatctttg aagagatcct
     4381 ctccggaccc ggtcctcgat ctccccggcc aggtcattag aaatgccggt gagtgcggac
     4441 tccgatcagt acttgcgctc atcggacttt caatgagcag cccgatcgag gtcctttcct
     4501 acgagtctcc cttcggggta ggttatgcca cggctcttct ggacctgtcc ggggagggag
     4561 aataatggac gggctcgcga gatcggtgaa aggcgaggca cacccctatc tggaccttgc
     4621 aaggagagct gtcaggctca ccgctctcgg gcaggaccct ttcaggcatc ttgatccccg
     4681 tgaatacaat gcaacgacgg agatgttaat agagcggggg tgtttcgtct cgatcaggat
     4741 ggcttcaacg ggagaactga gagggtgcat aggcacgata agaccggtcc gaaagagtct
     4801 ctgggaagag atcgttgcca atgcagccgc cgcggcgtcg gaggacccca ggttctttcc
     4861 ccttggaccg gacgaggcgg aggagtgccg ggtttcagtg gatgtacttg acgagccctg
     4921 tcctgtcggg gacctttctg aactggaccc cttacttttc ggggtgatcg ttgagaagga
     4981 ggggagaaaa ggggtccttc tccctgatct tgatggggtg gacactgttg aacagcagct
     5041 gtcgatcgcc atgaggaagg ccgggatcgg atcgtccgat ggcgtaagga tatttcgctt
     5101 cacagtccgt cgattctccg agtaaagaca ggagaagggt tgttgatcaa tggacaggac
     5161 ggcactttac tggatcgcaa ggggagatga tatcaactgc catctctgcc ccagggagtg
     5221 tatcataagc caggggctca cgggagcttg cggggtcagg gagaactccg gcggaaggct
     5281 tgtctccctg aactatggtt gggcgtgtgt cgccgtggac cctgtcgaga aaaaacctct
     5341 ctaccactgg agacccggca cgcggatcct ctccctgggg actttcgggt gcaatctttt
     5401 ctgtccctac tgccagaatt accatctgtc acgttcaaaa aaccataagg gcctctcctg
     5461 gacgaacccc tccgatgtac ttagactctt gcagcatcac gacgctccct ctgtggcctt
     5521 cacatacaat gagcccaccg tctggtacga attcgtcagg gatacatcgc tcctgctgaa
     5581 aagagaaaag aagggggtcg ttctggtgac caacggctac atatcccgaa agcccctgga
     5641 ggaccttatc catcttgtag atgcaatgaa tattgacctt aaaggctttt ccgacagcac
     5701 ctactccatg ctcggaggca ctcttgctcc tgttctagac acggtccgga tcgccttcga
     5761 gagatcggta catgtagaga tcacccacct ggtcgtgccc gggatcaatg attccatcga
     5821 ggaattccgg catctggtcc gatggatcgc tggtatttcg gagagtatcc ctcttcatat
     5881 ctccaggtat ttccccgatt accgctgggc ccttcccccc actccgatac cctctatcag
     5941 ggtcagggag gagatcgctt cggaatttct tgaaaatgtc tattccggaa atctacagag
     6001 caggaataac actctctgcc cggtctgcgg tgaggtcctg gtggaaaggg atgacctcta
     6061 cagggccagt gtgcacctct tggaggatgg gacgtgtcca cgctgcagaa cccctcgggg
     6121 tattgtcatg tcgtgatttt ccatataatc tcttttcaag agggtttcaa tcgaccaacg
     6181 gggggcgatc ctttatggga tcttcggtac tcgaactgaa cggtcactcg ctaaggcttg
     6241 aagatgtagt cgatgttgcc cgtgaaggga ggaagatagc ccttgacagg tctgctgtcg
     6301 cctttgtgga gagaggttca aggatggtcc gttcctgggt ggaggatgga aggataatct
     6361 acggcatcac cactggattc ggagaccttg caagccaggt cataccccct gacaagagcg
     6421 agaaactcca ggaaaacctc cttacaagcc acgcctgcgg ggtaggggag cccttcccgg
     6481 aagagatcgt cagggcgata atgctattaa ggatcaactc actgataaga ggtttctcag
     6541 gcattagcat ggagaccctt tcccgtcttg ttgacttcct taacatcggg ttacacccag
     6601 tgatcccctg caaggggtcc gtgggcgcca gcggagacct ttgccccctc tcccatcttg
     6661 ccatttcgct tcttggcctt ggagaggtct ttttccaggg aaagaggatg aaggcttcag
     6721 aggccctcag gtccgcggga cttgaaccca tagccctggg ccccaaggaa ggtctggccc
     6781 tgaacaacgg aacggcggcg atgaccggcg tagccgtcat ggccgtatgg gaagcgatgg
     6841 ccgtgctgaa gacggccgac atcgtgggag ccatgtcgct ggaagccctt cacggtgttc
     6901 ccttcgcttt cgaccacagg actcacgaac tcaggcccca ccagggtcag ataaaggtgg
     6961 ccaagaacat ccggggtctt gtcagtggca gccggatcct tgaaaaattc ggtaacgaaa
     7021 gggtccagga tgcctattcg ctccgctgta tcccccaggt ccacggagca agccgagacg
     7081 gtatccagta cgtcaagaac gtgatccaga tcgagataaa ctccgtcaca gacaacccgc
     7141 ttatcttccc cgccgagggt gaggccatca gcggcgggaa cttccacggt cagcccgtag
     7201 cccttgctat ggactttttc ggaatggcaa tggccgagat aggcaacatt tcagagagaa
     7261 ggactgccag gctggtggac ggaaagcttt ctggccttcc ccccttcctg gtcgagaaca
     7321 gcggtatcaa cagcggcttt atgatccccc agtatgttgc ggccgccctt gtctcggaga
     7381 acaaggtact tgcccaccca tcctccgtgg attctatccc cacatcggca aaccaggagg
     7441 accacgtttc catggggatg tactcagcca ggaaaggaaa ggagatcctg gacaatgtca
     7501 agaagatcct cgccatcgaa cttcttcttg ccgcacaggg gctggatttt tccagccctc
     7561 ttgaacctgg agccggtaca ggggctgcct acagaaaact cagggaggag atcccctacc
     7621 tagaaaagga tgttttcctt caccccatga tagacaggat aatcaaaggg gtggaggacc
     7681 gaagctacct caaggccgtg gaagaggcaa cgggggaact ggaatgattt ttcaagagca
     7741 aaggaacaga gaggaggaca agtatgcctg acaacagaag aattgaagca gctatgagaa
     7801 tatccctcgg agatgagctc ccgtctccgc ctcccttcga tccacagatc agacgggcac
     7861 ccgacagggg ttaccggctg gacaaaagac agacccttac gtctcttaag aatgccctca
     7921 ggtacattcc cgagaagctg caccaggtcc ttgcaccgga attccttgat gaactcagga
     7981 gccgggggag aatttacggt taccggtacc gtccatcaac cccccccaag ggtctgccca
     8041 ttgacagcta caaaggcaaa tgtctggagg gaaaggcctt ccaggtgatg atcgacaaca
     8101 acctcgatcc ggacgtggct ctttacccat acgagctggt cacttatgga gagacgggaa
     8161 gtgtctgcca gaactggatg cagtaccgcc tgatcaggaa gtacctcgag gaactgacag
     8221 aggatatgac cctcgttgtc gaatcggggc accctcttgg gctcttccgg tcgggacccg
     8281 atgccccaag ggtcattatc acaaactcca tgatggtagg aatgttcgac aaccaggacg
     8341 attgggagat cgcggcccag atgggggttg ccaattacgg ccagatgacc gccggtggct
     8401 ggatgtacat aggcccccag gggatagtcc acgggacctt caacactatc ctcaacgccg
     8461 gcaggaaact tggtctctcc acaggcaagg ggcttgaggg agtacttttt gtctcttccg
     8521 gcctcggcgg gatgagtgga gcccaaccga aatcggctga gatcgccggg tgcgtgggga
     8581 taattgcaga agtggacatc tccaggatcg agacccggca caagcagggc tgggtctcgg
     8641 tggtctcaag cgatcttgat gaggtcttcc ggatcgcggg agagaacctg tcatcccgga
     8701 agggaatctc catcgcctat cacgggaaca tagttgagct tctgcaatat gctgtggaca
     8761 gaaacgtcaa gatcgacctt ctttcggacc agacctcgtg ccacgctcct tatgacggtg
     8821 gttactgtcc cttccagcta tccttcgacg agaggacgaa gatgctcagg aaggacagaa
     8881 agatattccg ccagatggta gacgaaagcc ttagcaggca tttcgacctt atccgcacca
     8941 ttcatgaaag ggggacctac ttctttgact acggaaactc cttcatgaaa gccgtctttg
     9001 acgccggaag gctggatatt gccaaaaacg gcaaggatac cagggaggga tttatcttcc
     9061 cctcctacgt tgaggatatc atgggacccg aacttttcga tttcggctat ggccccttca
     9121 ggtgggtctg cctgagcgga aggccggaag acctcgaaaa gacagaccag gcggccatgg
     9181 agtgcataga tcctgagagg agaccccagg atcgggataa ctacctctgg gtccgggatg
     9241 ccgggaggaa ccgcctggtg gtaggaaccc aggcaaggat actttaccag gatgctgaag
     9301 gacgggtaag gatagccctc aggttcaacg aaatggtccg ccatggcgag atcgggccag
     9361 taatgctcgg tagggatcac cacgacactg gcggcacaga ttcgcctttc agggagacgg
     9421 ccaacatcaa ggatggcagt aacgtcatgg ccgacatggc catccagtgt tttgccggaa
     9481 acgcggctag agggatgagc ctcgtcgctc ttcacaatgg tggcggtgtc gggatcggga
     9541 aagccgtaaa tggagggttc gggcttgtcc ttgatggcag cgaaagggtg gatgaggtga
     9601 tcagacgatc cctctcctgg gacgttatcg gaggggtcgc ccgtcgttcc tgggcaagga
     9661 atcccaactc cattgagacc attatcggac acaacgcatc ctccggtgat catgtcaccc
     9721 ttcccttcct tgccgatgac aggttccttc tgtcactgat agaagaatag ccggggtcca
     9781 gtgaaggatc cctgggacgg aggatgatca tgaggatcgt tgagtgtgtc ccaaatttca
     9841 gcgagggaag aagaaaggat gtaatagaga gtatcgtggc acctcttcgc gacagaaaag
     9901 gttgtcaact gctcgatcac aggtccgacg agaaccataa caggctggtg gtaagcctgg
     9961 ccggcgaccc cgaggcgata atggattccc tgataaggtc ggcggaagcg gccttctcgc
    10021 ttatcgacat gaacgttcat tccggtgccc acccaaggat aggcgccctt gatgttgtac
    10081 cctttacgcc gctgaaaggt ttgtccatgg aggagtgcgt ccagatagcc cgggaattcg
    10141 gaagacgcct taacgaacag ttcaaggtgc cagtctatta ttacgaggag gcagccacaa
    10201 ggccggacag gaagaacctt gagaacattc ggaaaggcca gtttgagggg ttgaagaagg
    10261 cgatcggtga tcccgaaaga catcccgatg tcggagaggc caaaatgcac cctacggcag
    10321 gggcgactgt cgttggggct agaaaattcc tggttgcctt caacgtcaat ctgggcacct
    10381 gccgtattga aatcgcaaaa gagatcgcaa ggtccataag gttctccggg ggaggtcttg
    10441 ctcacgtcaa ggccatcggc cttgccctcg aggagaaggg gatggtccag gtaagtgtaa
    10501 acatagtgga tcacgagaag aaccccctgt acagggtcct cgaactggta aggatggaag
    10561 cctcgaggtg gggtgtcccc gtggtcgaga ccgagatcta cgggatggtc ccggcctcgg
    10621 cgatattcca gagcgctgct cattacctgc aggcggccgg cctcgaacct gaacagatca
    10681 tcgagctcaa gctcctcgat atgtcaggtg aacagtcatg acaacaaaac ttttcaggga
    10741 cgccaggatc catacccctg tctccgatgg aggcgggtgc gtatcgtggg agaggggagc
    10801 cctactcgtc agggatggtc tcatagaaag ggtcgggaga gaagatgacg tcgtccaggg
    10861 cctggacccg ttgcagatag accaggagat cggtctcgga ggcagatgtg tcgtccccgg
    10921 cttcgtagac cctcataccc atatgtgctt ttcagagctg agagagaaag agttcgccac
    10981 gcggctctcg ggggtaccat acctggagat cctcaggtca ggggggggga tcctttcgtc
    11041 ggtccggtcg gtgaggagcg cttgcgacga gactatcctt gggagaaccc tgaagaacat
    11101 acatatatct ctctctcacg ggaccactac ggtcgagatc aagagcggct acgggctgga
    11161 taccgcttct gagatacgcc tgctaaggct gatcgaaagg gtggggagac agacccccgt
    11221 cacggtcgta ccgaccttca tgggggccca tgcggtaccc tccgaattca ctgacgatcc
    11281 cgaagggtac gtcgatcttc tggtcaaaga gatgatcccc gccgtatccg ccgaatgcac
    11341 cccccccttc tgcgatgttt tctgcgagga aagtgtcttc tccgttccac agaccagaag
    11401 gatcctggat actgccagag agcacggaat gaaactcaag gtccacgccg acgaggttat
    11461 cgacacaggc ggcgcagcgc tggcagcgga gatgggtgct gtctcggcag agcacctcct
    11521 tgcagcaaga gaggagaacc ttgcgaagat ggcctcggcg ggggtcattg cggtcctgct
    11581 tcccgccacg gcctacagtc tccgcaaacc atatgccgac gcaaggaaga tgcttgacct
    11641 gggacttact gtggctctgg ccacggactg caaccccggt tcctccttta cccagtcggt
    11701 tcctttcgta ttcggcctcg ctgtcatgaa catgggcatg accgttgacg aagcccttta
    11761 cggatgcact ctcaatgccg caaaggctat cggggtcgac ggatcctgtg gaagcctcga
    11821 aaggaacaag cttgctgacc tggttgtgct ggacggagac acacctgcca ttctggctta
    11881 taattgcgga gttgctccgg tcctctccgt ctacaagagg ggtgagtgtg tcgtacaaag
    11941 aaccgacgat aggaggatca actgatgaaa gatatcgctt ccctgcctgt aggggatctt
    12001 attgaagagc tgggttcatc cagtgccact cccggagggg gctccgcttc ggccctgtgc
    12061 gcttccatcg ctgccagcct gacggcaatg gtggcaaacc tgaccgttgg acgctcccgt
    12121 ttctccgggg tccaggaaga gatggtccag gccgcagaaa ggggcaaggc cttatcgcgg
    12181 gagttcctcg atcttgccat gaaggattgc gacgcttttg accgtttcat gaaagccctg
    12241 gccctcccca aagactcaga gcaggagaga gagaccagga ggatatccat gcaggaggcc
    12301 ctcaaaggat cgacccttgt ccccatgagg atggtcgaac tcgccggtga tgtggccgag
    12361 aactgtcttg ccgtcgcctc catgggcaac cggaacgcca taacagacgc aggagtgtca
    12421 gctttgctga taaaagcggc ctctcgtgga gcggcaatga acgccaggat caacctgtcg
    12481 tccatcgagg acggatcttt tgtcgaagag aatctccgcc gtctcgaggc ggaccttgaa
    12541 aggaccggaa cagccgccga caaggtcctg gcagcggtat cttcggaact tccccggtaa
    12601 tgacaaagga taccggacca gccaccgttt cctgggcagg cagggtagtt catcttattc
    12661 tacttcttgt tacttttgcc tcctttattg ctcttgccca gtatctgccc ctgaaactgg
    12721 ccgggaccat tcagatgggc agaggcgatc ctatggatgt tctcgttctt ccaggaatga
    12781 acgccaggga gatctccagg gtcttcggat cgtcagggtt gactgaccgt cccaacgaac
    12841 tttcaaggtg gttcgcaaga gaaggtatcg acaggtcgat tcggcccgga ctctaccgta
    12901 tccgcaaggg ttcgccctgg gagatagccc ggcagatggc agtagcggag ccggaggtct
    12961 cggggatcac cctttacccc ggggagactt acgaagttct tcttgccagg ctcggtgaga
    13021 ctttcgaggg ctcccttgca agggatgacc tcttcccgga tacgttgcgc cccctccttc
    13081 catcagggcc aggttcgagg atcgcctttc tcatccccga gacctacagt gttactcctt
    13141 ccgatggagc cgtcaacgaa ctggtcaggg cagcctcggc gctctggatg aagagggtag
    13201 gtgagagagt tcttggcccg ggggtcgatg ccgacgcgct ccttgaaagg gctatcgtag
    13261 cctctcttgt ggaaagggag gcaaggcatg accacgagag gccaataatc tccggagtga
    13321 tccgcaaccg ctctgaagca gggatgaaac ttcagataga tgccaccgtg gtctatgcat
    13381 ggtctctgag gggagttgaa ctgaagaggg tcctctaccg ggacctggag atcgattccc
    13441 ctttcaatac ctaccgttat gccggcctgc ctcctgcccc tatctgtaca ccttcggagg
    13501 gatcatggct ggcttccatc gaacccgagg atgttccctt cctctactac gtggcaatgc
    13561 ctgacggtac acaccttttc agcaggacct tctcagagca ctccgaggcc gtcaggcggt
    13621 cgaggaatgc cttcggggcg gtccaccagt gagctgccta cggaaaaggg aggtctctga
    13681 aggtatggac agactttttg atgttaccga tgccatttca attcccggca cctccttcgg
    13741 agaggtgttc atccagagaa gtttcgcgcg ccactgcctc ctggatgacg ggaaattcga
    13801 agaggtctcc tcttctctga cggaggggac tgggacaagg atagtagtgg gtgacaggac
    13861 ctattttacc cattcttcag ggtcggacct ttcctcggca gcctttacca tctccgacgg
    13921 aaagaagcgt gccggccttt cggcagggag cccactcccg aagggtgaca aatcgctcct
    13981 gcctcttcca gagatagaag aggaagctcc cttcgagagg atgcgcgaac tggacaagat
    14041 gatcagaaag ctctctccca aggtgcgaca ggtatccttc gcgtggagca gcggagccag
    14101 agagacagcc atagccggca gccttaaccc cttgtgccgg ctatcggtct tttccacaag
    14161 attctccgcc acagtggtcg ttgagtccgg aggatccctt cactccggaa gggatgtcag
    14221 ggcattcagc ctgccccagg aagaattcct ccgatcaaac gatcttgagg atgtggcccg
    14281 ggaagctttc agaagggcag tgcttctctc ggaggcaaaa ccctgccctg cgggtgtaat
    14341 gcccgtggtg ctggcgggcg cagccggtgg gaccatgatc catgaagcct gcggtcacag
    14401 cctggaagcc gatattgttc tgaacgatta ttcgtccttc agggactgcc tggagaaaca
    14461 ggtggcatcc cctcttgtct cgcttgttga cgatccgact cttccgggac tttacgggag
    14521 ctaccccttt gacggtgaag ggaccctttc ccggagaaca gtcctcatca gggatgggtt
    14581 tctcaaatct ttccttactg acctcctctc ttcaagacag ggagggttcc cggtcacagg
    14641 gaacggaaga aggtcatcct tcaggcatcc tccggttcca aggatgtcca atacctttat
    14701 aatgaacggt gacggggatc ccgaagagat catctccggt gtggataggg gtcttcttgt
    14761 cctgaaaatg ggcgggggtg aggtaaactc cacatcaggg gattttgtct ttcaagttac
    14821 cgaaggctac cttgtttcca aaggcaaaag gtcataccct gtcaggaacg ccatgctgac
    14881 cgggaacggc cccagggtgc tgatggatat cgatgccgta ggcaacgatc ttcattttga
    14941 ccctggcgta tgcggcaagt cgggccagca ggtccccgtg accgacggcc agcctacgat
    15001 acggataagg cagcttgtag tgggaggaag cagaacgtga tatgaaggtg accaggagta
    15061 aaccccggaa caaggaaaag accggggccc gagaaccggt cagggccgaa gggaggagac
    15121 cgccatggtt caccatgatc gttcttgtcc ttcttctggt cgacctttac ctcctggcaa
    15181 cgctgtttct cttcaggact ggtgatctcg gacggatagt ccatttctgg accctggaga
    15241 tcttcggggg cggggtcatc gtactcctgc tctttggtgc ctactcctcg atctcatggc
    15301 tgatgggata ccaggtcccc tccccctgga ggcaggctct cgcttcgttg ataatgtatc
    15361 tgtgctgtgc ccttcttctt ggactctcca ccctgagcgg tatcgaaccc gttgtcggta
    15421 ttcttcgtcc cggccggccg ggctacctgc tggccggggt tctcttaaga cacatcggtc
    15481 ccttcggtgc cgttctggcg ggtttcggag gcatggcatc ggccatggtg ctttacggga
    15541 tcatcaacag gaggaacgtg gcagcggccg cttcccttct ctcaggtgtc ttgagatcct
    15601 tttgcgcact tctttcgccc ctttctggag gacatccagc cgggaccgct ccttcacagg
    15661 aaggaacgaa aaagaccccg ggcaagaccg ccccggagac cgtgacaaaa actggcagga
    15721 catccggttc cgcgaccccc ggcaagatag atattcccga tacgatagtg gatgaaaccg
    15781 cagtcgataa ggtccagaac ctttctgaca cgatccctgg caagacgctc tctttcccaa
    15841 tcccgctgga tgtcttcggc gaacccgacg atttctcctt cgaggtcgac aagggccttc
    15901 tttcccggca gggggagaac atcgttgata ctctcgagga gttcgggatc agcgccgaac
    15961 tggcggagac ggtggtcggc cccacggtga tccagtacag gatacagctt gccccgggca
    16021 tcaaggtcag cagggtgtca agcctggcca acgacctggc cgtatccctg gcagtcccca
    16081 gtctcagggt cgaagccccg atccccggga agacctatat cgggatcgag atcccgaacc
    16141 ctgacaggaa accggtgaat ataaggaccg ttctcgagca ctcctctttc caggattcgg
    16201 acagtgacct tgccattcct gtaggggtcg gtgtcgacgg ccgtcatctt accatcgggc
    16261 tggagaaaat gccccacctc ctggtggccg gaacgaccgg atcgggcaag agcgtttttg
    16321 tctcatgctg tattgcagcc cttgcatgcc gcatgagacc tgaagagctc cgtttccttc
    16381 tcatcgaccc aaagagggtg gagatgaagg tcttcgaacg gttgccccac aatatcaccc
    16441 cgcccgtcgt ggaatcgaga caggctgttc acgccctggg gtgggccata agggagatgg
    16501 agaagcgtta cgagatgttc gcccaggcca gggtaaggaa cataaaggct ttcaacgcca
    16561 gggccctgcc cctggaccga cttccctaca taattatcgt gatcgacgaa cttgctgacc
    16621 tgatgttcac atcaccccgg gaggtggagg actatatctg cagacttgcg cagatggcaa
    16681 gggccaccgg aattcacctg gtgatagcca cccagagacc ttcggtcaat gtcgtaacgg
    16741 ggctgataaa ggccaatatc cccgcaaggg tggccttcac acttccttcc cagaccgact
    16801 cgaggaccat aatagatgtc tctggagccg agagacttct tggaagcggg gacatgctct
    16861 ttctgacccc caggcttccc aagccgctaa ggatacagtc tccctggatg gacgagccgg
    16921 tgatacagcg tttcatggaa tacctttcgg ccctttttgg agaccctgtt ttccttgaca
    16981 tcgacatgca ggaggatgat gaacaggacg gaggccgggt ccatctggat gaccccctcc
    17041 tggaggaggc catggggctt atccttcaaa gcgggatagc ctcggcaagc aggcttcagc
    17101 gacagctccg gataggtttc acgagagcgg cacggctcat cgacaccctt gaacaggtgg
    17161 gcatcctggg cccccaggaa ggatcgagac cgagggatat cctggtggac gaggaagagg
    17221 caagacagat cctcggagcc agccttaaca ccccctgagg aacctgaccc ccatggaagc
    17281 aatggtcctg ccccagatca atgcacattt cgaacccgca atagaaccgc cttcaacggt
    17341 gatcgttgcg ggcggaaggg cgccgggtat ctcctggctg agagaggtct gctgctccag
    17401 ggatgtctgg gcggtggatc gtggccttga cagctgcatg tcggcggaca ttgtgccctc
    17461 tcttttcatc ggcgacgggg acagcgtgtc cagaaaggcc atgcgatggt cgcagttgaa
    17521 acgggttacc tccatactct gcccccctga aaagaacctt accgaccttc agctggctct
    17581 caaggaagca ggcggtaagg gttcctctgc catcataaca ggggcattcg gagggcggtt
    17641 cgaccatctc tttgccaacg tctactctct catatgggca gaagaggagt ggggcccgag
    17701 agtgcgctgc atggcagacg agaaggaggt gttctttctg ataagggggg gcgaatctct
    17761 ttccctgtcg ggacttgaaa gagggactgt cttttctact ctggcgctct ccgaggagtg
    17821 cagtggaata gacatgaaag gcgcgaaatg gcaggtaaag ggtggaagac tcttactcag
    17881 ggaacccttt gccataagca atatcttatc cggtgatgca gaaggggccc tgttcgacag
    17941 gaccccttta agggtaagtc tttcttccgg gtggctggga gtatacttta tgaccagcct
    18001 ttcagcagaa caagaccaga aaggccgctt ctagacggcc cttctgaccc ttcccgagcg
    18061 gaggcacttc gtacatatct tcatcctccg tgattcgttg cagcccacat caaccttgac
    18121 gttctgaagg ttgatgcccc aggtccttct tgtatgacgg ttggagtggc tgacactgtt
    18181 gccggtaacc cggcctcttc cgcagcaatc gcagacattg gccatttcca tccctcctgt
    18241 tcctgaacat cgggcaaacg gcgaacattg taccacgggt ggacaagggt gcgcaagata
    18301 acccccttcg ttgtatcatt atcctgtcca tggtgaatct atttcaacag ggagtgatcc
    18361 atttatgtcc ttcaccaaag acatgaccga tcttcaggag attgtctcca ggctggactc
    18421 cggaaagatc ccccttgagg aatctctcga gctcttcgag agggggatcg gacttgtcaa
    18481 ttcatgcagg aagtaccttg aagaggccca acagaagatc accctcctca ccgacgacaa
    18541 cggggaccat gagggcaggg agtgggatcc cctttcggga gatggcccga tcgatggaaa
    18601 ttaactttcc tcctgtgaag gaaaaccccc acgacaggat ctcggggtgc agatttttcg
    18661 ttgacaaggc aatagcagac ctctgtaaag agcggcctga aaggataccg gaaagacttt
    18721 gggagtcgat gacctactcg ctcacagcag gaggcaagag ggtcagaccc gtcctgtgca
    18781 ttctggcagg agaggccttc ggcgcctcaa gagaggatct catcccgatg gccctggcct
    18841 gcgaaatggt ccatacggca tcccttatcc acgatgatct tcctgcaatg gacaacgaca
    18901 gcatgagaag gggcaagcct accaatcacg tgatcttcgg agagcctttg gcacttctgg
    18961 cgggtgactc tctcttcctt tgggccttcg aatatgcccg caagggactc cttgcaggga
    19021 atgccttccc ccatatatcc atcctggagg cccttggagt cctgctcgat gcttcgggac
    19081 ccgcaggtat atgcggggga caggtccttg atacggaccc tgtcagcgca ggacccgagt
    19141 tggaacatcc atggaccgta gcgcggtcca agactgcggt gcttataagc gcatcgatcc
    19201 aggcaggggc gatactcgga ggagccgaac cccgtgagag ggaagccttt gcggaattcg
    19261 gagaccatct cggaacggca ttccagatag tggatgacat tatcgatgtg acaggggagg
    19321 ctgcaaggat cgggaagacg cccggaaagg acaggatcct gggaaaggtc accttcgtaa
    19381 gcgttttcgg tcttgaaaaa gctagaagga tggccagaca ggagacattc atggcactgg
    19441 agtccctgtc gaccattaaa aaggacgtgg ccgacctgga gaggttcccc cggatcctgg
    19501 cagaaaggga cagttgatcg ggttttggag acagagaccg gggagatgag agaattgctt
    19561 gacaggatcg tggatcacag ggacctgaaa gaacttgact atcggcaact ggatagcctg
    19621 gccgaagaga tcagggagac gatagtagga acggtccagg ctaacggggg gcatcttgcc
    19681 tcttccctgg gcgccgttga gctgaatata gccctgttga gggcctttga cccctcgaga
    19741 gacaggatag tctttgacgt cggacaccag gcctacgcgt acaagatcct caccggcaga
    19801 aaggaccagt tccacacg
//
LOCUS       DAOPKQ010000003        18773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_3094_length_18773_cov_5.499679, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000003 DAOPKQ010000000
VERSION     DAOPKQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 18773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18773
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3094_length_18773_cov_5.499679"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK146_00235"
     CDS             <1..198
                     /locus_tag="PK146_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE90693.1"
                     /translation="AIMGETPVENFLIDAGWSGHGLQFAPSAGRIISEIVMGEDPFLD
                     ISCFRYSRFEEGCLFPEPACI"
     gene            complement(293..763)
                     /locus_tag="PK146_00240"
     CDS             complement(293..763)
                     /locus_tag="PK146_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPE90694.1"
                     /translation="MKQGSFDMKKALVYAIHAEIEATEFYSEWAKNAQGHLRKELEEL
                     AKWEDGHRDSLTKYFEDTYNEKFARNPKMVVDPALKVQADEFKDNYQLLKIASTVYLS
                     EMKAMELYQEMENESSGKSKKMFEELKNMEKDHMVTAKKRYDEIREHIVGFRAF"
     gene            complement(860..1756)
                     /locus_tag="PK146_00245"
     CDS             complement(860..1756)
                     /locus_tag="PK146_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPE90695.1"
                     /translation="MDEKVLALKVDVDTLKGYLEGTPRLLGVFGKLEVRASFFFSFGP
                     DNSGKAIKRVFRKGFINKMLRTNAPGTYGLKTMMYGTILPAPLIVPRDPALFRRAISE
                     GHECGIHAWDHVAWQDELDRMADATMREHFARAFDMFRDLSGGEPHCCAAPAWKVTAA
                     SLTMQDEYGFDYCSDTRGSSPFIPRMGGMTFKTPQVPTTLPTMDELLGSDGLDDDSIN
                     SHLLDLIKPGLNVHTIHTEMEGRAKARILEDFLSRCIGLGIPVIPVGEALKNLQGGDL
                     PFSDVVDREIPGRAGKVAVQSG"
     gene            2046..2762
                     /locus_tag="PK146_00250"
     CDS             2046..2762
                     /locus_tag="PK146_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE90696.1"
                     /translation="MTPDCPFNMVSLRQQVYDYLRSQMNQGELRPGSAINLGEMSSRF
                     GISRTPLREALLQLETEGFVTIYPRKGIIVNPLSEEDIRHAYEIIGALEASVVFNERN
                     SITSTETEKMRRLNLLMREALDRDDFDTFYRHNISFHNIYLDLSRNRSLVRTVMTLKQ
                     RLYDFPRRKGFVKEWEITSTGEHERFIELLETGDAKAASDYMRDVHWSFEVQRPWIHR
                     YYRELAREEALERSAEGVPR"
     gene            2759..3664
                     /locus_tag="PK146_00255"
     CDS             2759..3664
                     /locus_tag="PK146_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPE90697.1"
                     /translation="MNRGVIFDIKRYSIHDGPGIRTTVFLKGCPLSCWWCHNPESQSA
                     FTEVVYRPDRCIGCGACEGACPVGAIALTQRGYIADPDRCTGCGRCAEICPAEAREMV
                     GREITVQDLVEEIEKDSLFFDESGGGVTFSGGEPLSQSAFLAEALEACRERDIHTVVD
                     TCGFASRQVLERIAPLTELFLFDLKMMDPELHKRYTGVPNGLILSNLEWLAGQGFSMI
                     IRVPVIPGINDTAENIEATGKFLSELSRRPGVDLLPYHSSAREKYRRFGMNYRLEDLS
                     APSRERMEIIAAYLARFGITASIGG"
     gene            3670..6030
                     /locus_tag="PK146_00260"
     CDS             3670..6030
                     /locus_tag="PK146_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HPE90698.1"
                     /translation="MNERIERLRIESFEAPVTLDHERAEIITDFYRENNGKYSVPVTR
                     ALAFRTLCEKKNIFIGKDELVVGERGPLPKCVSTYPELNCHSLEDLEILDSRDKNPYR
                     VSGKCKEVYRDKVIPYWRGRTMRDRIFADIGEEWLETYATGYFTEFMEQRAPGHTTLD
                     GKFYSKGMEDFKKEIAETISCLDFKNDPEATKKREQLKAMDIACDAVIIFAERHADLA
                     SGMAASESDPSRRKELEKIASNCRQVPRWAPRDFWEALQMYWFMHLGTITELNGWDAM
                     NPGHLDRYFYPFYARELESGTLSREWAKELLSAFWIKFNNHPAPPKVGVTAAESGTYN
                     DFTNINLGGLLEDGTDGVNEISYMMLEVLDEIHLLQPQANIQLSRKNPDRFLKAALRV
                     IRKGYGYPSIFNADTVIEQMIRAGKSVKDAREGGTSGCVETGAFGKEAYILTGYLNTP
                     KIFELALNNGVDPVSGKRIGPETGDPAQFRTFDDLNGAFEKQLNHIVEIKVKGNQFIE
                     RMYADYAPAVFLSVLTDDCIKKGRDYNDGGPRYNTNYIQCVGIGTMTDSLSALKKHVF
                     EEKAFSMEDVLQALRSNFEGDEVLRLTFANKTPRWGNDDDYADDIMKRVFESLFSEID
                     GRPNTKGGRYHIDMLPTTCHIYFGSVTGATPDGRQAGLPLSEGISPVQGADRNGPTAV
                     IRSCGKMDQIRTGGTLLNMKFMPRVLEGDEGIEKLARLVRTYFRYDAHHIQFNVVDSK
                     TLRDAQVHPEEYRDLIVRVAGYSDYFCDIGLDLQEEIISRTAQEEV"
     gene            6151..7299
                     /locus_tag="PK146_00265"
     CDS             6151..7299
                     /locus_tag="PK146_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE90699.1"
                     /translation="MRIFSFISFLGIVLGVYGVANLYIWLHLAKVVPYLGALRVPVVL
                     AFWTLVAAFPVGRIGGAFCRCTFTDTLAIVGSWYLGFMVYVLLLLAILDIVRIADHWF
                     RIVPYSLNRRPGRAALVAFWSVLSLAVLVVAAGRWNAITPVVREYDIVLRGSSKERPL
                     RVAVATDLHVGLLVRNHWLSRMIETINSTRPDMVLLVGDIVDSDVTHAQEERLSEELG
                     TLSAPLGVFAVLGNHEFYSGAEEAAASFRDGGVTVLRDEAVQVEGILTLVGRDDRASA
                     RRGSSRFPLSAIPGVEGSLPVIVMDHTPSDLQEAEDAGVSLQVSGHTHRGQLWPFNFM
                     TSRIFEQDWGFLKKGETLYYISCGVGVWGPPIRTSSRPEVVVLNVSFQ"
     gene            7339..8361
                     /locus_tag="PK146_00270"
     CDS             7339..8361
                     /locus_tag="PK146_00270"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPE90700.1"
                     /translation="MPTCKELCGNLSSRTLRWVVTGGAGFIGSHLVESLLSLGQKVTV
                     LDNFLSGRESNVEEARKLSTGRDPSFRLLKGDIRDMEQCREACHGADIVLHHAALVSV
                     PRSFQEPHLNNSCNITGFLNMLTAARDEGVRSFVYASSAAVYGHQPEGPTGEDAPLSP
                     LSPYAVAKRTDELYASVLSGGMRTTGLRYMNVFGPRQDPSSPYSGVISIWSRCLMDGE
                     PPVIYGDGKTTRDFVYVGDVVQANLLAALSDLRSGDDENIFNIGTGRPVSLEKAFLAM
                     SGAIESLCPGWKGKEPVHEPERMGDIRYSLADISRARELLGYEPRYSLEEGLVLTIKS
                     FMNSRM"
     gene            complement(8750..10132)
                     /locus_tag="PK146_00275"
     CDS             complement(8750..10132)
                     /locus_tag="PK146_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPE90701.1"
                     /translation="MKLVANIFREYDIRGNAEEELTSETVRAIGLAYGTFLSREGVRK
                     ITVGGDVRLSTDRIKGDIIEGVTSAGVDVVDIGMVTSPLLYWSLFHLDLDGGVMITGS
                     HNPKEFNGLKLAFGKTTLYGDQIQEILRMIEADDFHVADEKGSVTMMDLSDEYLAMLE
                     SKIRLGKRKLKVVTDTGNATAGPMVNRFLRELGCEVVPLFEEPDGTFPNHHPDPTKRE
                     YVKDLIDTVKQEKADLGLGFDGDADRIGVIDDRGEMVWGDILMILFWREILPRNPGAE
                     AIVEVKSSQALVDEIKRLGGKVVWWKSGHSLIKAKMKEIDALFSGEVSGHMFFADEFW
                     GFDDSFYAAGRLLRILSNTDRKLSGLLEDVPVYHSTAETRITCADDRKFGVIESIRED
                     ALKNHEAITIDGVRILYPRGWGLVRASNTQPVIVARAEGKTEKDLAEYSADLKERILK
                     AGVENFEWEY"
     gene            complement(10175..11197)
                     /locus_tag="PK146_00280"
     CDS             complement(10175..11197)
                     /locus_tag="PK146_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HPE90702.1"
                     /translation="MKILILGANGFIGSHLCEHLLDRTDWEITAFDINDFNLGKCMGK
                     ERFSFRKGDFYTDDRWIEGALNECDAVLPLVGIAKPAFYITNPVMTFHLDFEANLKIV
                     RMCVKHGKRVIFPSTSEVYGMSTDTELKEDESPIILGPVCKSRWIYSCSKQMMDRVIA
                     AYGQEEELRYTLFRPFNWIGPRLDTFEDAKEHKARSITQILYDILHERDVSLVGGGRQ
                     RRSFTWIGDGIEGLEAIIRNENGCADGEIFNIGNPRNNASIKELALVMIRVLKSHPKY
                     GPLAEKARLLEIDSRDYYGKGYEDVLDRVPSVAKANRLLGWEPKTPLDEAVSRTIEAT
                     LMENGL"
     gene            complement(11534..12421)
                     /locus_tag="PK146_00285"
     CDS             complement(11534..12421)
                     /locus_tag="PK146_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HPE90703.1"
                     /translation="MSDRPGIVVFAYSEVGYRCLKVLIDLGANVRGVFTYDDDPGENA
                     WFRSVKELALSGGIPAFTTRGLRDPGTVELIENLEPELMFSFYYRDMIPQRILDIPRL
                     GAFNMHGSLLPRYRGRACVNWAIIHGETETGATLHWMTSRPDDGDIVDQERVPITGTD
                     TAMDVMLKVAEAAERLIARNLPLLERGEAPRIPQDHPKASYFGGRRPEDGRIDWNASA
                     MDICNLVRAVTRPYPGAFTFLDGEKVFIWKATPLNENSGAAPGTVISRDPLVIAAGEG
                     SVRVEEKSQAAGDRKSLNP"
     gene            complement(12414..13355)
                     /locus_tag="PK146_00290"
     CDS             complement(12414..13355)
                     /locus_tag="PK146_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE90704.1"
                     /translation="MEKTAPEVSVVIPVYNEEESLPGLFERLLPVMEGLDRSWEVVFV
                     NDGSRDRSLALLLDCVDKHPGAVRVVDFNGNFGQHMAIMAGFSVSRGNAIVTMDADLQ
                     NPPEEIPRLLEEMDRGHDVVGTIRSTRKDPFFRKFASKVVNRITNRITGLNLHDYGCM
                     LRAYSRRVVDLINSCPESTTFIPALAQKFALNPSEIEVGHSERELGTSKYSLFRLIRL
                     NFDLMTGFSLVPLQAVTMLGLVIAGLSFVFSIYMLLRRILVGPEAEGLFTLMTINFFL
                     MGVTMFCVGIAGEYIGRIFQEIRKRPRYLVDRIYGDE"
     gene            complement(13364..14527)
                     /locus_tag="PK146_00295"
     CDS             complement(13364..14527)
                     /locus_tag="PK146_00295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPE90705.1"
                     /translation="MRETFLPFARPDISEDAIADVADSLRSGWITTGPKTQKFEKSFG
                     EYVGSPYSLAVNSATAGLHLSLLAFGIGPGDEVITTPMTFAATLNTVVLTGATPVLAD
                     IDPVTLNIRPDMIERAITSRTRAVIPVHFAGRPCDMQAIEEIVQKNDLVLIEDAAHAL
                     GASCRGKLIGSPAGERHAAVFSFHPTKNITCGEGGMVCTGCEEAAEKISVLRQHGMSK
                     GAWNRYAATGTPHYDILFPGLKYNMLDIQAAIGSDQLTRLDTFNERRREIVDIYRKEL
                     GELKGLRLPPEPDEGDVHSWHIFTPLLDIDLLGFSRDDFMARMREMNIGTALHYQAAH
                     LFSYFGQNYRWKRGDFPGAEHVSDRIVSLPLFPAMTDGDVTDVVEAVRQICGR"
     gene            complement(14524..16179)
                     /locus_tag="PK146_00300"
     CDS             complement(14524..16179)
                     /locus_tag="PK146_00300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPE90706.1"
                     /translation="MFSSHRKNLLFLLVLTSILYLVALGSHGLLDPDEGRYSEIPREM
                     IESGDFITPRLNYVAYFEKPVLHYWLTALSFKALGQNEFAARFWPAVLAIAGVFVTYW
                     LANRMNGPRAAFLSSWILATSLVYFAIGQINITDMPLSFFITLSLAGFWMGHRRDRRY
                     YLLFYLGMALATLTKGLIGIVLPAGVVFWWIVMTRKWSVIREALYLPGIVVFFLTATP
                     WFVLVCLENPDFFDFFFIQEHFLRYTTKIHDRFEPCWWFIPIIIVGFIPWTGFLPGAV
                     SGALPSSLARPTHQDRGNLFLLLWFGIIFIFFSLSSSKLIPYIIPVMPPLAILAGSFL
                     DLHMDKGKTRSLGMGLLLNSLILVPFGVAAAAYPFLDERYGKVLLPFVAPVVAVLILL
                     VVLSWVYYLKGQSRRVVLTLCIFAFLNIATFKNVFGFYDGIMSARDLASVIRRHALPD
                     DVIAQYRDYDQGLPFYLERRIVLVDWRGELEFGANRGDQSDWFLDSEGFIERYWTSDE
                     RVLLVMREEQSTELFETRGVTPVILARVSGKLVVTNKGDEKDQ"
     gene            16297..16662
                     /locus_tag="PK146_00305"
     CDS             16297..16662
                     /locus_tag="PK146_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HPE90707.1"
                     /translation="MDRLGMLMILGSALFNATASSMMKIGFGHQQDLLERGFAGAVFR
                     IAGNPWAVAGVVCFGISFMFMSAALTRVDLSVAYPVMSGLVFILVLGVSSVFFAEQIT
                     LMRLIGVFLILSGVLAISR"
     gene            16908..18236
                     /locus_tag="PK146_00310"
     CDS             16908..18236
                     /locus_tag="PK146_00310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPE90708.1"
                     /translation="MKVCIIGTGYVGLVTGACLAENGNDVWCVDIDQAKIAGLKEGRV
                     PIYEPGLEDMVKRNLTENRLRFTTDMKEGLDPSLFVFIAVGTPPMEDGSADLGHVYEA
                     ASQIGSLMDGNKIVVAKSTIPVGTTERIRGIITERLAERGRRGVEFDVAFCPEFLKEG
                     AAVEDCLRPDRIIIGTGNERTAELLRELFSPFVMKENRFYTMSIPSAEMTKYASNAML
                     ATRISFMNELSRLCEKVGADVGQVRVGMGTDSRIGSSFLYAGLGYGGSCFPKDVRALI
                     NCASSKKVDLSILRAVEEANRTQRQFFLDKMIGHFGEELSGRTFALWGLSFKPHTDDL
                     REAPSLTISDALLSRGAALRVFDPVAMEKARTILPDDGSVTFCSDAYEALEGANALVL
                     VTEWPQFRRPDWDKIRDLMKPAAAVFDGRNQLSPAHVRALGFSYYGIGRG"
     gene            complement(18445..>18773)
                     /locus_tag="PK146_00315"
     CDS             complement(18445..>18773)
                     /locus_tag="PK146_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPE90709.1"
                     /translation="QLTIITTPLLRGRIPCAVDSVYTVSTPGEVVDAVVTEFGVAINP
                     RRQDLINAAKEAGDVPLVTMDELIETAEKFTGPMSPVETEDRIIGVVEYRDGTVIDVV
                     RQLKKK"
BASE COUNT         3988 a         5329 c         5285 g         4171 t
ORIGIN      
        1 gccatcatgg gtgaaacgcc ggtggagaac ttccttatcg atgcggggtg gagcggccat
       61 gggctgcagt tcgccccttc ggcggggcgc ataatctccg agatagtcat gggcgaggat
      121 cctttcctgg acataagctg cttccgttac agcaggttcg aggaaggctg cctcttcccg
      181 gagccggcct gcatctgatc cgggaaaagg tcacaaatct gtaagaaaga acgccggagg
      241 cggtattgca gcccccggcg ttccctgttt gagatgactg cttttctttc gatcagaagg
      301 cgcggaaacc gacgatgtgc tccctgatct cgtcgtaacg tttcttggcg gttaccatgt
      361 ggtccttttc catattcttc agttcctcga acatcttctt ggatttcccg gagctctcgt
      421 tctccatctc ctggtatagc tccatggcct tcatttccga aaggtagacc gtggaggcta
      481 tcttgagcag ctggtagttg tccttgaact cgtcagcctg gaccttcagc gccgggtcga
      541 ccaccatctt gggattcctg gcgaacttct cgttgtaggt gtcctcgaag tactttgtga
      601 gcgaatccct gtgcccgtcc tcccacttgg cgagctcctc gagctctttc ctgagatgcc
      661 cctgggcgtt cttggcccac tctgagtaga attctgtggc ttcgatctcg gcgtggatgg
      721 cgtatacaag ggctttcttc atgtcgaacg aaccttgttt catatcctat ccctccttaa
      781 aaaaagataa ctcctgttcc caaataatat accctatggg gctacttccg tccagttata
      841 cttttgtcgg tttgacagat catcctgatt ggacggccac tttcccggcc cttcccggta
      901 tctcccggtc cacaacatca ctgaagggga ggtcaccccc ctgaagattc tttaaggcct
      961 ctccgacggg gatcacaggg attccgaggc cgatgcaccg ggaaagaaag tcctccagga
     1021 tccgtgcctt ggctcttccc tccatctcgg tgtggatcgt gtgtacgttc aggcccggtt
     1081 ttatgaggtc cagcaggtgg gagttgatac tgtcgtcgtc gagcccgtcc gaacccagga
     1141 gttcatccat ggtggggaga gtggtgggga cctggggagt cttgaaggtc atcccgccca
     1201 tcctggggat gaagggggag ctaccccggg tatcgctgca gtagtcgaat ccgtactcgt
     1261 cctgcatggt caggcttgca gctgtgacct tccacgcagg ggcagcgcag cagtgaggtt
     1321 ctcctccgga gaggtcccgg aacatgtcga aggctcttgc gaaatgctct ctcatagtag
     1381 catcggccat cctgtccagc tcatcctgcc atgccacgtg gtcccaggcg tggatgccgc
     1441 actcgtgacc ctccgaaatc gcccggcgga acagggccgg gtcgcggggg acgatgagcg
     1501 gtgccggaag gatggtcccg tacatcatgg tcttgagccc gtaggtaccg ggggcgttgg
     1561 tacggagcat cttgtttatg aagccctttc ggaacactct cttaatggct ttgccggaat
     1621 tgtcgggacc gaaagagaag aagaacgagg cccggacttc cagctttccg aagactccta
     1681 gcagcctggg cgtcccttcc aggtatcctt taagggtgtc cacgtcgacc ttgagcgcca
     1741 gaaccttttc atccaccgga tatcatctcc ctgtgcaatt gtagcagggg tcaggaagaa
     1801 taaattgaaa ggcggaaact gtacgacatt cagacgtggg tattctgcgg cgattttggt
     1861 ttgctttcac cgtagacgtc tttgagtttt gccctttccg tctcagaaaa gctgagatca
     1921 aaacctggat tgaacgaaat gtcagcttga catcacttag tatgtctgct gccctgttaa
     1981 agatcttccc ttgcccttcc ctgatatctg atatattata tatctgtatt gagaggaggc
     2041 gcgaaatgac ccccgactgc cctttcaaca tggtgtctct cagacagcag gtctacgact
     2101 atctcaggag ccagatgaac cagggcgagc tgcgtcccgg tagtgcgatc aaccttggag
     2161 agatgagttc ccgcttcggc ataagccgta ctcccttgag agaggccctg cttcagctcg
     2221 agaccgaggg ttttgtcacc atctaccccc gcaaggggat catcgtgaac cccctctctg
     2281 aagaggatat ccgtcacgcc tacgagatca ttggggccct ggaggcctcg gtagtgttca
     2341 atgaacggaa cagcatcaca tcgaccgaga cagaaaagat gcgacgcctg aacctgctta
     2401 tgcgggaggc gctggaccgg gatgatttcg acaccttcta ccgtcacaac atctccttcc
     2461 acaacatcta ccttgacctt tccaggaacc ggagcctggt ccgaacagtc atgaccctga
     2521 agcagaggct ctacgatttc cccaggcgca agggtttcgt caaggagtgg gagataacct
     2581 ccaccggcga acacgaacgt ttcatcgaac ttctggagac gggggatgca aaggccgcct
     2641 cagattacat gcgggatgtc cactggtcct tcgaggtcca gaggccctgg atccaccggt
     2701 actacagaga actggccagg gaggaagccc ttgaaagaag tgccgagggg gttccacgat
     2761 gaacaggggc gttatcttcg acatcaagag gtactccata cacgacggac ccggtatcag
     2821 gacgacggta tttctcaagg gttgccccct ctcctgctgg tggtgccata atcccgagag
     2881 ccagtcggcc tttaccgagg tcgtctaccg ccccgaccgc tgcataggct gcggggcctg
     2941 tgagggtgcc tgtcctgtgg gcgcgatcgc cctcacccag agaggctaca ttgcagatcc
     3001 tgacaggtgc acgggctgcg gaaggtgtgc cgagatctgc cctgctgagg cgcgggagat
     3061 ggtaggcagg gagatcaccg tacaggatct ggtcgaagag attgagaagg actccctgtt
     3121 cttcgacgag tccggaggag gggttacctt ttcaggcggg gaacctttgt cgcagtcggc
     3181 tttccttgcc gaagcacttg aagcctgccg cgaaagggat atacacaccg tagtcgatac
     3241 ctgcggtttt gcgtcccgcc aggtcctgga gaggatcgcc cctcttacgg aactcttcct
     3301 cttcgacctt aagatgatgg accccgaact gcacaaacgt tacaccggtg tcccgaacgg
     3361 tctgatcctg tcaaacctgg aatggcttgc aggccagggt ttctcaatga tcatcagggt
     3421 ccccgtaata cctggtatta acgacacggc ggagaacatc gaggctacgg ggaagttcct
     3481 gtcggaattg tcccggagac ccggggttga tctcctcccc taccactctt cggcccggga
     3541 gaagtaccgc aggtttggca tgaactaccg tctggaagac ctttccgcac cgtctcggga
     3601 gcggatggag ataatagcag cctatcttgc ccggttcggg attaccgcat ccataggagg
     3661 ttgaagaaaa tgaacgaaag gatagagagg ctcagaattg agagtttcga ggcacccgtc
     3721 acactggatc atgagagggc tgagataatc accgacttct acagggagaa caacggcaag
     3781 tactctgtcc ccgtgacaag agccctggcc ttcaggaccc tctgtgagaa gaagaacatc
     3841 ttcataggca aggacgagct cgttgtgggg gagcgtggcc ccctgccgaa gtgcgtctca
     3901 acttatcctg aactcaactg ccactccctg gaggaccttg agatcctcga tagcagggac
     3961 aagaacccct acagggtctc gggaaagtgc aaggaggtct accgggacaa agtcataccc
     4021 tactggcgag gccgcaccat gcgcgacagg atattcgccg acataggaga agagtggctg
     4081 gagacctacg ctaccggcta cttcaccgag ttcatggagc agagggctcc gggtcacacc
     4141 acgctggatg gcaagttcta ctcgaaaggc atggaggatt tcaagaaaga gatcgcagaa
     4201 acgatctcct gcctggattt caagaacgat cccgaagcga cgaaaaagcg tgagcagctt
     4261 aaggcaatgg acatagcctg cgacgccgtt atcatcttcg cagaacgcca cgccgacctg
     4321 gcatccggaa tggccgcttc cgagagcgac ccgtcacgca gaaaggaact ggagaaaata
     4381 gcctccaact gcagacaggt cccccgttgg gcaccaaggg acttctggga ggcccttcag
     4441 atgtactggt tcatgcacct cggtaccata accgaactca acggctggga tgccatgaac
     4501 ccggggcacc tggaccgcta cttctaccct ttctacgccc gggaactgga gagcgggacc
     4561 ctttcccgcg agtgggcgaa ggagctcctg agcgccttct ggatcaagtt caacaatcac
     4621 cccgccccgc ccaaggtggg tgtcacggct gccgagagcg ggacctacaa cgacttcacc
     4681 aacatcaatc tgggcggact gctcgaggac gggaccgacg gggtcaacga gatatcctac
     4741 atgatgctgg aggtgcttga cgagatccac ctcctccagc cccaggccaa tatccagctg
     4801 agccgcaaga accccgacag gttcctcaag gcagccctca gggtgatccg taagggttac
     4861 ggctatccat ccatattcaa cgccgacacg gtgatcgaac agatgatccg tgcagggaaa
     4921 tccgtaaagg atgcccggga gggcggcacc agcggctgcg tggaaactgg agccttcggc
     4981 aaggaggcat acatactgac gggctatctc aataccccca agatctttga gctggctctg
     5041 aacaacggtg tcgacccagt atcaggcaag aggataggcc cggagacggg cgatccggcc
     5101 cagttcagga ccttcgatga tctcaacgga gccttcgaga agcagctgaa ccatatagtc
     5161 gagatcaagg tcaaggggaa ccagtttatc gaacggatgt acgccgacta tgctccggcg
     5221 gtgttcctgt ctgtgctgac cgacgactgc atcaaaaagg gcagggacta caacgacgga
     5281 ggtccgaggt acaacacaaa ctacatccag tgcgtcggca taggcacaat gacggacagc
     5341 ctcagcgccc tcaagaaaca cgtcttcgaa gagaaggcct tctccatgga ggatgttctc
     5401 caggctctca ggtccaactt cgagggtgac gaggtactga gattgacctt cgccaacaag
     5461 acccccaggt ggggcaatga tgacgactat gcggacgaca tcatgaagag ggtcttcgaa
     5521 tcgctcttca gcgagatcga cgggaggccc aacaccaagg gcggccgtta ccatatcgac
     5581 atgcttccca ccacttgtca catctacttc ggttctgtca ccggcgcaac gcccgatgga
     5641 aggcaagcgg gtttgcccct ctccgagggg atctctccag ttcagggcgc cgaccgcaac
     5701 ggccccacgg cagtgataag gtcctgcgga aagatggacc agatccgcac cggcgggacc
     5761 ctgttgaaca tgaagttcat gcccagggtc ctcgagggtg acgaagggat cgaaaagctt
     5821 gcccggctcg ttaggaccta cttccgctac gatgcccacc atatccagtt caacgtcgtc
     5881 gacagcaaga cgctgaggga tgcccaggta caccccgagg aataccggga cctgatagtc
     5941 agggttgccg ggtacagcga ctacttctgc gacattggac tggacttgca ggaggagata
     6001 atctcccgca cggcgcagga agaggtttag ccttaaaggc gtcaacggta aacagtgaac
     6061 tgtaaacggt aagagcgcaa gacctgttcc tccggttttg tccttgtcac tgtatttacc
     6121 gttcacgctt ttgactacct tggaggttca atgagaattt tttcctttat ctccttcctg
     6181 gggatagtcc ttggcgttta cggggtcgcg aacctgtata tctggcttca cctggcaaag
     6241 gtggtccctt acctgggtgc cctcagggtc cccgtcgtcc ttgctttctg gaccctggtg
     6301 gcagccttcc cggtcggcag gataggaggt gccttctgtc gctgtacctt cacggatacc
     6361 ctggctattg tcggttcatg gtatctgggg ttcatggtat atgtcctcct gcttctggct
     6421 attctggaca tcgtaaggat cgccgaccac tggttcagga tagttcccta ctcgctcaac
     6481 agaagaccgg gccgggccgc ccttgtcgct ttctggtctg tcctgtccct ggctgttctt
     6541 gttgttgcgg cgggacgctg gaatgccatt actccagtgg tcagggagta cgacatagtc
     6601 ctcagaggca gctccaagga gaggcctctg agagtggccg tggccaccga ccttcacgta
     6661 gggcttttgg tgaggaatca ctggctgagc agaatgatcg agacgatcaa ttcgacccgt
     6721 cccgacatgg tcctgctcgt gggagacatc gtcgactcag atgttaccca tgcccaggaa
     6781 gagaggctct ccgaagagct ggggaccctt tcggccccgc tgggagtttt tgccgtcctg
     6841 ggcaaccatg agttctactc tggagcggaa gaggccgcgg catcctttcg tgacggggga
     6901 gttaccgttc tgagagacga ggctgtccag gtggagggta tcctgaccct tgtcggccga
     6961 gacgaccgtg cttcggcaag aagaggcagc agcaggttcc ccctttcggc gatcccaggc
     7021 gtcgaaggtt ccctcccggt catcgttatg gaccataccc cttcggacct tcaggaggcc
     7081 gaggatgctg gtgtctccct ccaggtctca gggcacaccc acaggggtca gctttggccc
     7141 ttcaacttca tgacttcgag gatctttgaa caggactggg gtttcctgaa gaaaggcgag
     7201 accctttact atatctcctg cggagtaggg gtatggggac ctcccatcag aacctccagc
     7261 cgtccagagg ttgttgtatt aaatgtaagt ttccaataga attacccctg ttgactgaga
     7321 tccttgcggg ggtggaccat gccaacgtgc aaagagcttt gcggaaacct ttcatccaga
     7381 accttaaggt gggtagtaac aggaggggca ggttttatcg gttctcatct tgtagagtcc
     7441 cttctgtccc tggggcagaa ggtaaccgtc ctggataatt tcctctccgg aagggagtcc
     7501 aatgtcgaag aggcacgaaa gctctccaca ggccgggatc ccagtttccg tctcctcaag
     7561 ggtgacataa gggacatgga acagtgccgg gaggcctgtc acggcgccga tatcgtcctg
     7621 caccatgcgg cactggtctc ggtccccagg tccttccagg agccccacct taacaactcc
     7681 tgtaacataa caggctttct taacatgctg accgctgctc gtgacgaggg tgtgaggtct
     7741 ttcgtttacg cctcctccgc cgcagtatac gggcaccagc ctgaggggcc cacaggagag
     7801 gatgcccccc tcagtcccct ctcgccctac gccgtagcga agaggacgga cgaactctat
     7861 gcgtcagtgc tatccggcgg catgaggaca accggactgc gatacatgaa tgttttcggt
     7921 ccgaggcagg acccttcgag cccatactcc ggggtcatat cgatctggtc caggtgtctc
     7981 atggatggtg agccccctgt gatctacggc gacggaaaga ccacaagaga cttcgtctat
     8041 gtcggggacg tcgtccaggc caaccttctg gcggctctgt ccgatctcag gtccggggat
     8101 gatgaaaaca ttttcaacat aggcacgggg cggcccgtct cgcttgaaaa ggcctttctg
     8161 gcgatgtccg gtgcgataga aagcctttgc ccgggctgga aagggaagga acctgtccac
     8221 gagcctgaaa ggatgggcga catcaggtac tccctggccg acatcagccg cgctcgtgaa
     8281 ctcctgggat atgaaccacg gtactctctt gaggaagggc tcgttctcac gataaagagt
     8341 tttatgaatt caaggatgtg aagttacttt ttctgaaagg aaaagcgtaa acggtcaaca
     8401 gtaaacggaa gggtcaaaac taaaaaccaa taccttttca ccgcagagga cgcagagaaa
     8461 agcaagagac aaaaacacaa tttatggtta aggtaccaga accaagatca ggcgtctcac
     8521 gaaaaaacaa ggcacctcgt tggatcatct tcctcgttta agcacctcgg gaacttgtct
     8581 gcggtgaaaa gggtttttta agtgggttcc ccaattaacg cttttgtctc tctctgcggc
     8641 cctctgtgtc ctctgcggtg aaatggtttg gaatttgatc ttcctgtcgc cttgttccag
     8701 gttttccgtc taacgtctaa catttcacgt ctcacgaatt taaagattgc taatactccc
     8761 actcgaaatt ctcgaccccg gccttcagta tccgttcctt caggtccgcc gaatactccg
     8821 ccaggtcctt ttcggtcttt ccttcagccc ttgccacaat aacgggttgt gtgttggaag
     8881 ccctgaccag tccccacccc ctggggtaaa ggatcctcac cccgtctatg gtgatggctt
     8941 catggttctt gagtgcgtcc tcccttatgc tctcgatgac accgaacttt ctgtcgtccg
     9001 cgcaggttat gcgcgtctcc gccgtgctgt ggtagacggg aacgtcctcc agcagtcccg
     9061 agagtttccg gtcggtgttg gacagtatcc tcaaaagtct tcccgcggcg tagaaggagt
     9121 cgtcaaagcc ccagaactca tcggcaaaga acatatgccc cgacacctca ccggagaaga
     9181 gggcatcgat ctccttcatc tttgccttga taagtgagtg tcccgacttc caccagacta
     9241 cctttcctcc caggcgtttt atctcatcca ccagtgcctg ggaactcttg acctcgacaa
     9301 tagcctctgc tccagggttc cggggaagga tctctctcca gaagaggatc atcaggatgt
     9361 caccccagac catctcccca cgatcatcga tgacgccgat ccggtctgcg tccccgtcaa
     9421 aaccaagtcc caggtcggcc ttctcttgct tgacggtatc tatgaggtcc ttgacgtact
     9481 cccgcttcgt cggatcggga tgatggttcg ggaaggttcc gtcgggctcc tcgaagaggg
     9541 ggaccacctc gcaccctaac tctctgagga acctgttgac catgggacct gccgtggcgt
     9601 tccccgtgtc tgttacaacc ttgagctttc tctttcccag tcgtatcttc gattccagca
     9661 ttgccaggta ctcatcagag aggtccatca tcgtgacgga acccttttcg tctgccacat
     9721 ggaagtcatc ggcctcgatc atcctgagta tctcctggat ctggtcgccg tagagggtgg
     9781 tcttgccgaa ggccagcttg aggccgttga actccttggg attatggctt ccggtgatca
     9841 ttacgcctcc gtcaaggtcc aggtggaaga ggctccagta gagcagcgga gaagtgacca
     9901 tgccgatatc gaccacgtcg actccggccg aggttacacc ttcgatgatg tcgcctttga
     9961 tcctgtccgt tgaaaggcgg acatctccgc ccacggtgat cttccggacc ccctcgcgcg
    10021 aaaggaacgt tccgtaggcc aggccgatgg ccctgacggt ctcgcttgta agctcctcct
    10081 cggcattgcc ccttatgtcg tactccctga agatgttggc aacaagcttc atcgggactc
    10141 ctcccagtta aaaagatcgc tcttcatcat agcactacag gccgttctcc atcagggtcg
    10201 cctcgatggt cctgctgaca gcctcgtcga ggggcgtctt cggctcccag ccgagaagcc
    10261 tattggcctt cgccaccgac ggcaccctgt ccagcacatc ctcgtaaccc ttgccgtagt
    10321 agtctcggga gtctatctcc aggagtcttg ccttctcggc cagcggaccg tacttcgggt
    10381 gcgatttcag gaccctgatc atcacaagtg caagttcctt tatggacgcg ttgttccggg
    10441 ggttgccgat gttgaatatt tccccgtcgg cgcagccgtt ctcattcctg attatggcct
    10501 caagtccctc gatcccgtcg ccgatccagg tgaagcttcg tctctggcgt ccccctccta
    10561 cgagggagac gtccctttca tgaagaatgt cgtagaggat ctgggtgatc gaacgtgcct
    10621 tgtgttcttt cgcatcctcg aaagtgtcga gcctcggtcc gatccagttg aaaggtctga
    10681 aaagggtgta tctgagttcc tcttcctgcc cgtaagccgc tattacccgg tccatcatct
    10741 gtttgctgca gctgtatatc cacctggatt tgcagacagg acccaggatt atagggctct
    10801 cgtcctcctt gagctcagtg tcggtgctca tcccgtagac ctccgaggtg gacgggaaga
    10861 tcacccgttt gccgtgtttc acgcacatcc ggactatctt gaggtttgcc tcgaagtcga
    10921 ggtggaaggt catgaccggg ttggtgatat agaatgcagg ctttgctatt ccgaccagag
    10981 ggagcactgc gtcgcactca ttcaaggccc cctctatcca tcggtcgtcg gtgtaaaagt
    11041 cgcccttccg gaaggagaac cgttccttgc ccatgcactt gccaaggttg aaatcgttga
    11101 tgtcgaaagc cgttatctcc cagtcggttc tgtccagaag atgctcgcac agatgagagc
    11161 ctatgaaacc gttggcaccg aggataagta tcttcacaaa aaacccccct gtttccgtaa
    11221 gtcgcgaacc gtaggatcaa gagcgtaaac ggtaatagca aactttaaag ccagggccaa
    11281 aggtgaaact taaagccctt tcaccacaga gaacacagag aagagcagga gagaggaaac
    11341 atagaacctc tttatggata aaatcagtca agtccggaac aaaaaccttc tatgactatt
    11401 ccgttcattg ttttcagttt aagccttctt ggccttggtc atggtcccta aaacaaatat
    11461 tgtgattttc tctcttgctc ttctctgtgt tctctgtggt gcaagatatg ggttctgttt
    11521 ttcttctaac gtctcacgga tttaaacttt tcctgtctcc tgccgcctgg ctcttctcct
    11581 ccacccttac cgatccttcc cccgcggcga tgaccagggg gtcacgggat ataaccgtcc
    11641 ccggggcggc cccgctgttc tcattgaggg gcgtcgcctt ccagatgaag actttctccc
    11701 cgtcgaggaa ggtgaaggca cccgggtagg gtcgcgtgac cgccctcacc aggttgcaga
    11761 tatccatcgc agaagcgttc cagtctatcc gtccgtcctc gggtctcctc cccccgaagt
    11821 agctcgcctt cggatggtcc tggggtatcc tgggagcttc ccctctttcc agaaggggga
    11881 ggttcctggc tatcaacctt tcggccgcct cggccacctt taacatcacg tccatggcgg
    11941 tgtcggtccc ggttatgggg accctctcct ggtccacgat gtctccatca tcggggcggg
    12001 aggtcatcca gtggagggtg gccccagtct ccgtctcccc gtggataatg gcccagttca
    12061 cgcaggctct tcccctgtac cttggaagta gagatccgtg catgttgaag gcaccgaggc
    12121 ggggtatatc gaggatccgc tgggggatca tatcgcgata gtagaaagag aacatcagtt
    12181 ccggttccag gttttcgatg agctcgaccg tccccgggtc cctaaggccg cgggtggtaa
    12241 aggcggggat acctcctgat agagcgagct ccttcaccga cctgaaccag gcattctcgc
    12301 ccgggtcatc gtcgtaggtg aagacacccc gtacattggc acccaggtct atcagaacct
    12361 tcaaacaacg gtaccccacc tcgctgtagg caaatacgac gatccccggc ctgtcactca
    12421 tcaccgtaga tcctgtccac caggtacctt gggcgcttcc ggatctcctg gaagatcctg
    12481 ccgatgtact ctccggcgat ccctacacag aacatggtca cgcccatgag gaagaagttg
    12541 atagtcatga gggtgaagag gccctcagcc tcgggcccca ccaggatcct cctcaagagc
    12601 atgtagatgg agaagacgaa gctcagccca gcgatcacga gaccgagcat ggtgaccgcc
    12661 tggagcggca ccagggagaa gcccgtcatg aggtcgaagt taagcctgat gagcctgaag
    12721 aggctgtact tggacgtccc cagctccctc tccgagtgcc cgacttctat ctcagagggg
    12781 ttcagggcga acttctgggc cagggcgggg atgaatgtgg tcgactcggg gcagctgttg
    12841 atcaggtcca ccacccgcct gctgtatgct cgcagcatgc agccgtagtc gtgaaggttt
    12901 agccccgtta ttctgttggt gatcctgttc accaccttgg aggcgaactt cctgaagaag
    12961 gggtcctttc tggtcgaacg aatggttccc accacgtcgt ggcccctgtc catctcctcc
    13021 aggagtctgg ggatctcttc cggtgggttc tgcaggtctg catccatggt cacgatggcg
    13081 ttgcctcttg atacggagaa tccggccatg atggccatgt gctgtccgaa gttcccgttg
    13141 aagtccacca cccttaccgc cccggggtgt ttgtcgacgc agtcgagtag cagggcgaga
    13201 gaacggtccc tgctaccgtc gtttacgaag accacctccc agctccggtc cagcccctcc
    13261 atgacgggca ggagcctttc gaagaggcca gggagtgact cctcctcgtt gtagactgga
    13321 ataaccactg aaacctccgg agcggtcttc tccaagggag tcgtcacctc ccgcatatct
    13381 gcctgaccgc ttcaaccacg tcggtcacat caccgtcggt catggcgggg aaaaggggca
    13441 gcgaaacgat ccggtcgcta acgtgctcgg ccccggggaa gtcgcccctc ttccatcggt
    13501 agttctgccc gaaataggag aagaggtggg ccgcctggta gtgaagggcc gtgccaatgt
    13561 tcatctccct catcctggcc atgaaatcgt cccgggagaa gccaaggaga tcgatatcca
    13621 ggaggggtgt gaagatatgc caggagtgga cgtcaccctc atctggttcg ggcggcagtc
    13681 tcagtccctt taactccccc agctccttcc tgtatatatc cacgatctcc cttctccttt
    13741 cgttgaaggt gtcgagccgg gttagctggt cgctcccaat ggctgcctgg atgtcgagca
    13801 tgttgtactt cagccccgga aagagaatgt cgtagtgagg tgttcccgtg gcagcgtagc
    13861 ggttccaggc ccccttgctc atcccgtgct gtctcaggac ggagatcttt tctgcggctt
    13921 cctcgcagcc cgtgcagacc atccccccct ccccgcaggt gatgttcttg gtggggtgga
    13981 agctgaagac ggcggcatgt ctctcgcctg caggggaacc tatgagcttg cccctgcagg
    14041 atgcaccgag tgcgtgagcg gcgtcctcga tcagtaccag gtcgttcttt tgaacgatct
    14101 cctcaatggc ctgcatatcg caggggcgcc ctgcgaagtg taccgggatg acggctctgg
    14161 tccgagaggt gatggccctc tctatcatat cgggccttat gttcagtgtt acggggtcga
    14221 tatctgcgag gacgggtgtg gcgcccgtca gaacgacggt gttcaacgtg gctgcaaagg
    14281 tcataggggt cgttatgact tcgtctccgg ggccgatgcc gaaggccagg agtgacaggt
    14341 gaagccctgc cgtagcggaa ttgaccgcca gggagtaggg tgaacctacg tactccccaa
    14401 aggacttctc gaacttctgt gtcttgggtc ccgtggtgat ccaccccgac ctcagtgaat
    14461 ccgccacgtc ggctatggca tcctcgctta tgtccggtct tgcgaatggc aggaacgttt
    14521 ccctcattgg tccttttcat ctcccttgtt ggtcaccaca agtttgcccg agacccgcgc
    14581 aaggatcaca ggggtcaccc cccgggtctc gaaaagttcg gtggactgtt cctcgcgcat
    14641 caccagcagt actctttcgt ctgaggtcca gtaacgttca ataaatccct cggagtccaa
    14701 aaaccagtcc gactggtccc ccctgttggc gccgaactcg agctcgcctc tccagtccac
    14761 aaggacgatc cttctctcca ggtagaaagg aagcccctgg tcgtagtcac ggtactgggc
    14821 gatcacatcg tccggaaggg cgtgtcggcg tatcaccgag gccaggtctc tggcgctcat
    14881 tatcccgtcg tagaagccga agacgttctt gaaggtcgct atgttcagga atgcgaaaat
    14941 acatagggtc agcacaaccc tccgggactg ccccttcagg tagtagaccc aggacagcac
    15001 gaccaggagg atcaggacgg ccaccacggg ggccacgaag gggagcagga ccttcccgta
    15061 tctttcatcc aggaaggggt aggctgccgc ggcgaccccg aaggggacta gtatcaggct
    15121 gttcagaaga agacccatcc ccagggacct ggtcttgccc ttgtccatgt gaaggtccag
    15181 gaatgaaccg gcaaggatcg cgaggggcgg catcaccggg atgatgtagg gtatcagctt
    15241 ggagctggag agagagaaga agatgaagat gatcccgaac cagaggagca aaaagaggtt
    15301 gcccctgtcc tggtgagtag gcctggcaag cgacgaaggc agggcgccgc ttacggcacc
    15361 cgggaggaat cccgtccacg ggatgaaccc cacgatgatg atcggaatga accaccaaca
    15421 gggttcaaag cggtcgtgaa tcttcgtggt atagcgcagg aagtgctcct ggatgaagaa
    15481 gaagtcgaag aaatccgggt tctccaggca caccagaacg aaccagggcg tcgcggtgag
    15541 gaagaagacc acaatgccgg gcaggtatag tgcttcccgt atcacactcc acttgcgggt
    15601 catcactatc caccagaaga ccacccctgc agggagaact atgcctatca ggcccttggt
    15661 gagagtggcc agggccatcc cgagatagaa gaggaggtag tacctcctgt ccctccggtg
    15721 tcccatccag aaacccgcca acgagagagt gatgaagaac gaaagcggca tgtcggttat
    15781 gttgatctgg cctatggcaa aatagaccag agaggtagcc aggatccacg acgacaggaa
    15841 ggcagccctt ggtccgttca tcctgttcgc aagccagtat gtgacgaaca cccctgcaat
    15901 tgccaacacg gcaggccaga agcgtgcggc gaactcgttc tgtcccagcg ccttgaatga
    15961 cagggcagtc agccagtagt gaaggactgg cttctcgaag taggccacgt agttcaggcg
    16021 gggggtgata aagtccccgc tctcgatcat ctcccggggt atctcggagt agcgcccttc
    16081 gtcggggtca aggaggccgt ggcttccgag ggccaccagg taaaggatcg atgtaagaac
    16141 caggaggaac agaagatttt tcctgtgaga agagaacaag gacaacagcc tccacaaagt
    16201 gaggatatct tgcctcgggg gcaccaggaa cacaaacttg gattattata acggtatagc
    16261 cagaagaatt accaacccgc aggaggtgct ttagccatgg accgactcgg gatgctcatg
    16321 atcctgggct ccgcgctttt caacgccacc gctagcagca tgatgaagat aggtttcggg
    16381 catcagcaag accttctcga gagggggttc gcgggtgcgg tcttcaggat agccggcaac
    16441 ccctgggctg ttgccggggt ggtatgtttc ggaatatcct tcatgttcat gagtgctgcc
    16501 ctgacgcggg tggacctgtc cgtggcctac ccggtgatgt cgggcctggt cttcatcctc
    16561 gttctcggtg tctcgtcggt cttcttcgcc gaacagataa cattgatgag gctgatcggg
    16621 gtctttctga tactgtcggg ggtcttggcg ataagcaggt agggaaccgg ttaacagtga
    16681 acggtaagtt caaattcaag gccggcgttt ccctgcttcc gtttacccag tccctcgtcg
    16741 ggaccagctg ccccgagcga agcgaaaggg taggtttcgc tctgcccgga gcgatagcga
    16801 ctgggtgccg tttacgcctt tgaatttttc gattcacaag tcacggcatt tttcgtagtc
    16861 taacgtcttt aacgtttttc ccatctcacg cctttggggg gttcatcatg aaggtctgca
    16921 tcataggaac cgggtacgtc ggacttgtca ccggggcatg tcttgcagag aatggcaacg
    16981 acgtctggtg cgtggacatc gaccaggcca agatagcggg tctgaaagag gggagggtcc
    17041 ccatctacga gccgggcctg gaggatatgg tcaagaggaa cctcaccgaa aaccgtctga
    17101 gattcactac tgatatgaag gaaggccttg atccaagcct gttcgttttt atagctgtgg
    17161 gaactccccc catggaagac ggctccgcgg acctgggcca cgtctacgaa gctgcctccc
    17221 agataggcag tctcatggac ggcaacaaga tcgtcgtagc caaatccacc atacccgtcg
    17281 gtactaccga gaggataagg gggatcatca ccgaaaggct agccgaaaga ggccgccggg
    17341 gtgttgagtt cgacgtcgcc ttctgccccg agttcctcaa ggaaggggcc gcggtcgagg
    17401 actgtctccg cccggacagg atcattattg ggaccggcaa cgaaaggaca gccgagttgc
    17461 ttcgggagct tttctccccc tttgtcatga aggagaaccg cttctacacc atgtccatac
    17521 catcggcgga gatgaccaag tacgcatcga acgcaatgct tgccacccgg atcagcttca
    17581 tgaacgagct ctcccgtctt tgcgaaaagg ttggagccga cgtggggcag gttcgtgtcg
    17641 gaatgggaac cgacagcagg ataggctcct ccttcctcta tgccggtctc ggctacggcg
    17701 gctcctgttt cccgaaggat gtccgggcac ttatcaactg tgcgtcgtcg aagaaggtgg
    17761 acctctccat tcttcgggcc gtcgaggagg cgaacaggac acagaggcag ttcttcctgg
    17821 acaagatgat cgggcacttc ggagaggaac tctcgggaag gaccttcgct ctttggggcc
    17881 tgagcttcaa acctcacacc gacgacctga gggaggcgcc ttcgctgacc atatccgatg
    17941 ctcttctttc gaggggagcc gccctgaggg tcttcgaccc tgtggcgatg gagaaggccc
    18001 ggacgatcct gcccgatgac ggttcggtga cattctgctc cgacgcctac gaggccctgg
    18061 agggcgccaa cgccctggtg cttgtcactg aatggcccca gttccgcagg cccgactggg
    18121 acaagatcag ggatcttatg aaacctgccg ctgctgtatt cgacggccgc aaccaactat
    18181 ctccagccca cgtcagggcc ctgggcttct cctattacgg gatagggagg gggtaaaaaa
    18241 ttattaaagg cgtgaacggt aaacagtaag cggttaacgg gatggatcaa acaaaagcct
    18301 gactggattt ttcccaggca ggaagtatct tgtggatagt gaacaaaggg aaaaggccag
    18361 gagaacaccc ggccttttcc tcacagatcc aaatgttttc ggcttttgcc atttaccgtt
    18421 taccgctaac cgttcaccgg tcttttactt tttcttcagc tgcctcacga cgtcgatgac
    18481 ggtgccgtcg cggtactcga cgaccccgat gatcctgtcc tctgtctcga ccggtgacat
    18541 ggggcctgtg aacttctccg ctgtttcgat cagttcgtcc atggtcacca gggggacatc
    18601 cccggcttcc tttgctgcgt tgatcagatc ctgtctcctg gggttgatgg ccacgccgaa
    18661 ctcggtaacc acggcatcga ccacttcgcc aggcgtgctg acagtgtaga ccgaatcgac
    18721 cgcgcagggt atgcgaccgc ggagcagagg agtggtgatg atggtcagct gcg
//
LOCUS       DAOPKQ010000004        18417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_3190_length_18417_cov_5.535236, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000004 DAOPKQ010000000
VERSION     DAOPKQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 18417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18417
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3190_length_18417_cov_5.535236"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /gene="dnaA"
                     /locus_tag="PK146_00320"
     CDS             complement(<1..1264)
                     /gene="dnaA"
                     /locus_tag="PK146_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPE90710.1"
                     /translation="MEDNTAQVWRKILEIAEDNLPKGAADIWLKTCLPVSLVDQVLTL
                     DVPNVFVREQIQSRFIEKLTALTSQKGLADRIVLEVGGAKKEEIKRAERISRESERPK
                     NGLNPDYQFDSFVVGKSNRLAHAASLAVAESPGVAYNPLFFWGGVGLGKTHLLHAIGN
                     FVTRHNEGTKIAYISSEKFINEFIMSIQNNKTHEFKLKYRSVDVLLIDDIHFLANKES
                     TQEEFFHTFNSLHDAKKQIVLTSDKPPKDIHNIEERLVSRFEWGLVTDIQPPDLETRI
                     AILKKKAALKNYFVPDDVIDFLAQNIPSNIRELEGSLNSVMFFADLNNESVTVENSSR
                     WLKDLIRRSSRGQITIELIQQATAESFGIPVSSLSGNKRTSEIATARQVAMFLSRELT
                     GTTLQQIGYAFNREGRLRVASEMKALADD"
     gene            1581..2591
                     /locus_tag="PK146_00325"
     CDS             1581..2591
                     /locus_tag="PK146_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPE90711.1"
                     /translation="MSRAGLHIISHTDLDGVTAAAVAWTYGRPLSGPLRVSLTGYGEV
                     DLLVLDSLSRGEPLVVLDLFCQRVQTVDEIDRSFSHGDPPFFFDHHQSTFERYGSRPW
                     VFVDRESCAAMVYYRWLMDNAAREDRPRLVPLEGIVEVANDRDLWLNRIPESRLWQAL
                     VTLCGPWAVLARLIEDPSPELSPEERSFSSAFVEEQEKRFRLALEKALRSGEDLLFVG
                     PGALEFGDVSDFCGLVLDRMESPPRLAAVLSRRPAGDWNVSMRSREGFAGRVTGLLRD
                     GKKVRGGGHDDSAALYFPHHYRPEEIRDSLVSAVRSAIESDAPSGLSLGDLLKQALEG
                     KE"
     gene            2613..3728
                     /locus_tag="PK146_00330"
     CDS             2613..3728
                     /locus_tag="PK146_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HPE90712.1"
                     /translation="MSFRVAILYASVGTGHRTAAMALEKWFQQEIPDPQVLCLDTLSF
                     SLPIVRGVYTRSYLEMVRHMPHLWGYFYETTDDPETRNGVIATLGELTEKLNILKLKK
                     TLLSFSPDAIVFTHFFGAAAIAESFAPDIPVCYVNTDFLSHVFHRNPAFSAWFVSSEE
                     TLNQYLADGLSLERVFLTGIPVDPAYASPPGREEARERLGLDMDERIALVMGGGIGVG
                     PIEEVVLSLHEGGFATEVICGINEKVRGVLLERFADRPGVRVHGFVDNMPCRYASADI
                     IFMKPGGLSTSEALCSGTPIVVTDPIPGQEQRNSDYLLDRGAARALFDYRLAAEKAHS
                     LLSTKEEKARVLRAAKRIARPMAGREIVRKVKEIKDQ"
     gene            complement(3749..5089)
                     /locus_tag="PK146_00335"
     CDS             complement(3749..5089)
                     /locus_tag="PK146_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE90713.1"
                     /translation="MCSRMRSSWLRASVQFGFLGFMTWLGYRHQVLGGGPSGVPTVDA
                     LCPFGGLEGLYSILASGTWLRRLAPSAMVLLVVLVVMTLLFGRIFCGWICPLGTLGEW
                     TSRLGSRLGIKKRELPPKVDGVLRWLKYLVLLVIIFATWKAGTLVWRGFDPWVAWMHL
                     SAGWEEFVESPWSYFSLFVLVIGLSLFIERFWCRYLCPLGAFLAPLQKISIFKIRRSE
                     DDCIHCHRCGPACPVRLDPEKKDVERSAECIACGRCVESCPREKALFFGTSSRKISVL
                     VLGILSISLFFGGYGLARGMGWWQTYAASSGASGGLAAPNLYGWMTLEDVSETLKVPV
                     QEIIGAVDLPEDFPTDVPLKNIAGIDDDEALLERILERTQIPSPTAPSNPDEIKASMT
                     IREISAVWGLDPVEILETAGWPADVSLDSTLKTLSGELDKGVTEIREAVKKLSK"
     gene            5224..6399
                     /locus_tag="PK146_00340"
     CDS             5224..6399
                     /locus_tag="PK146_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE90714.1"
                     /translation="MSIYLNNGATTWPKPPCVAEAVSAFILGSGANLARGSSSGRDIE
                     TLDMVTSTRQQVACFFRGYRKNDPRYVTFTGNVTESLNVVLKGYLRPGMHALTTSMEH
                     NSVIRPLRRLEGQGAKVSILECDNRGFLSPEVLHSFLKRERVDLFVLSHASNVCGSIQ
                     PLEDLAAICAGAGVPCVLDSAQTAGVTEIDVEGLGLAACCFTGHKGLMGPQGIGGIVW
                     EPGFAGRVLPFVEGGTGSFSDDEHQPEKLPDRFEAGTPNLPGIAGLSASLGWIAETGV
                     SAIFEREKELGMRFLEGIRKAPGIRIFGSENERSRLAVFAVNLSSMDNGTAAFELSER
                     WGIETRPGLHCSPLGHRTLGTFPSGALRISIGFFNTEQEIEVAIEALCTLAAENAPL"
     gene            6471..7448
                     /locus_tag="PK146_00345"
     CDS             6471..7448
                     /locus_tag="PK146_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HPE90715.1"
                     /translation="MQLDPQISDVLLAARFLRGKVRHTPTERSDSLSSLSGAEVFLKW
                     ENQQICSSFKVRGALNKMYSLSEEERAKGVVTASSGNHAQGVAMAARSLSARAAICVP
                     GVCPQTKRDAIMRLGGDFVELRVIGHLYDDAEDEAHRLQKEEGMTYVSSYEDHFIISG
                     AGTLGLEMIMDEPYLDTVIVPAGGGGLINGVAIAVKALSPETEIWGVQSVASNPYVVS
                     WPGGKVLDVSYEDSLADGLTGWIPQSLLDLSRKRVTNFVDVLESDIAKAIAFLHSRHH
                     QVVEGAGAVGVAAVMAGKVPVKGKRVGIVISGGNIDQPRLLEILREYSD"
     gene            complement(7528..10920)
                     /locus_tag="PK146_00350"
     CDS             complement(7528..10920)
                     /locus_tag="PK146_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPE90716.1"
                     /translation="MGKYWKALLILLILLSLAVGTALLLASRSDIGAGTVASELEKAA
                     VQHLQADLSLSSISGNPFRGFRIRDLSIRRDERLLFSAPEVAISFKPLSLLTGSPRIR
                     SISFGGALLEWPQVFPLLFKEGTPGTVSLPFDMFRVHRSIVRTPAGDIKVEEGRVILR
                     EGVIDASGSLVFRETTFSLKAELEMAGERLDLRDMSIRTTGGALTVSGRILPVADLTG
                     VVSDLDIKILERFWPDLAKQGYSGNLTTSFSARGAWPEVAVKGDLDIPQGEVYGIEME
                     GISSPWSFSGRALEIGEIRGKANRTAVSGKLQFVFSAMPPVTTIDITAREAEAKSWRR
                     SFSWLSLADGTIDSMEIRLQGPSNALSGTINISTPDFTLAGQPLSEIDGTLQLEKSST
                     IGLDLKAHWLGSPVTGKGDITIGQRPVFDLTLSGNDLDLSRASSVIPVENLALSGKAA
                     GSVRIFGAGQEIRSGGTINSARINAAGETIERPEISFAYSEGNITINSISALWRGSRV
                     AGSGRITGLVKEKGTFDLAGTLQEGPVSSVAGFVPALKELGLEGRVSAEWNLKGPVKT
                     PTLALEIRSPRVDVPEKASLTDMKILTKVALPLASETPDMRLDMAAERLQFNGGTLSN
                     LRTAFTVASGTVNIENAQGEILGARISVTGTARLPSSNRPGRTDLKGTASGVDLSVLG
                     NRIPFNVKGPVEASFTIKGDLPGPELAISGQSPSIVLAGLNLSDISFRVRGNGSEMTL
                     EELKGSAGEGVLTAKGTALFGPGGLDMDFSMEGSGLDLARITKEVKSNGPPDLSGTLD
                     VSLSGSMKDGRWNGRGEIFSERMTAFGIDFDDASLPVVLEEESIRTENAVGKFYGGTI
                     NAEGSIETATGRWNIRAAVRGADIDQTVRAVTDLDGSISGKGDLDLSLSGAFGRHVLV
                     TGRGNLSATEGEISGFNALKSISSAYGMSSIRYRMIDANFKIDGNVVTLLPGSRATAY
                     VDDPLYRYLNVDGPAGPGGNLNLYCSGLVNVQALNTLLGAIQGLATAGSASPEALLEG
                     LIGGVVGGLGRQDFRDTNFRIGGTWSNPSFHSFRIAPAQGAVQPLPESDGVGQTQQNG
                     TQHKVTISIPTGEGSGTPPDTGEDIKRQVIEQLLRNILPGNE"
     gene            complement(11181..11954)
                     /locus_tag="PK146_00355"
     CDS             complement(11181..11954)
                     /locus_tag="PK146_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPE90717.1"
                     /translation="MKSLAALCCLLILFSPLQAGAGTALWGRVVEVVDGDTVTVETSD
                     GKVEQVRYIGIDCPETSHPRRRVEELGREASRFNRSLVLGRRVLLELDLQKKDRYGRT
                     LAYVWVESDEGSFMANELLVSSGLAVPYAIPPNLRYTDLFREAFASARSSGRGLWEKA
                     RGRLFTPTQVWAELPSLAGRFINIRFKVDSISSSRTRYTLRPDKGYTTLIIHKSDTGQ
                     FGSIEDLAGRTLIVTGKVIPGFNGPEVILSDRTQILSFY"
     gene            complement(11951..12376)
                     /locus_tag="PK146_00360"
     CDS             complement(11951..12376)
                     /locus_tag="PK146_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HPE90718.1"
                     /translation="MPVKRGKNLIALRLDEGEDLIPSLLEALGDTTSAVIISAIGMLR
                     DIEIGWLGPDGYEKHQFSDPHEILSISGTVNKKSDGKAFIHAHASLSGRNMSVIGGHL
                     FGGTVNNTCEMVFLVPEGLTFERKVLVKGEPPRFCPENK"
     gene            12528..13229
                     /gene="rsmG"
                     /locus_tag="PK146_00365"
     CDS             12528..13229
                     /gene="rsmG"
                     /locus_tag="PK146_00365"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPE90719.1"
                     /translation="MPEPFPEQRLSTEGLLVEMEKNREGLERYALLLASFNERARLTG
                     PRDPSEILGHIRDCAVSLVFAPDIGTVVDIGTGGGLPGIVWALCRSNLKITLLESVGK
                     KCAILEEMAGMLSLVNVDVVCSRSEALSLERRERYQIALSRAVGHLGVVAEYASPLLA
                     VGGSACLFKGPRVLQELREIGDRWATLGFDDPVIHPYDLDGKSPCLVRLQKTGPCPAR
                     FPRNPGKANKSAWWR"
     gene            13226..14005
                     /locus_tag="PK146_00370"
     CDS             13226..14005
                     /locus_tag="PK146_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE90720.1"
                     /translation="MKLLKTVAVANQKGGVGKTTCCVNLAAELGRQGKKVLVLDIDPQ
                     GNSTSGLGVDVANIKNSMYHVILDEVSPTNAIIQSSWEGVWVLPATLDLAGSEVELAG
                     VMSRETRLARHMNAMKDFDIGFIDCPPSPGLLTVNALVASDSLMVPIQCEYYALEGVG
                     QLMRTVSLVQRYLNENLKLDGVVLTMFDTRTNLSREVADEVKNQFKEAVFETIIPRNV
                     RLSEAPSYGKPICYYDQGSAGAAAFRNLAEEVQDRWLAQGH"
     gene            13984..14871
                     /locus_tag="PK146_00375"
     CDS             13984..14871
                     /locus_tag="PK146_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPE90721.1"
                     /translation="MARPRALGKGLGALIPGAGADPIPVAGAPGEVLHLRLKDIRPSP
                     NQPRKDFGREGLEALADSIREHGIVQPVVVRKLNGGYEIVAGERRWRAAELAGLEEVP
                     VRVIDADENRVMELSLVENLQREDLSPLEAARGIRELVEKFSLTQEQAAKKLGWSRVA
                     VSNKLRLLNLPEEILEMLHRGDLSEGHARALLSLEDLLEQIHLARQCVDRGMSVREVE
                     EAVRKPSRGKDNRARRSSQSVLIPEPVLRVSERYGIDVKMSGRGDNVRVMLGGLSEDE
                     ARRLFEMIDRSGEILFPDK"
     gene            14891..15115
                     /locus_tag="PK146_00380"
     CDS             14891..15115
                     /locus_tag="PK146_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90722.1"
                     /translation="MARLTEDLGAFTRVLSSALLLCGFIIFGVVGGRALVLRGYPGWT
                     LPAAIIAGTAFGAWQAWSFLKTGMKRGRNS"
     gene            15229..15726
                     /locus_tag="PK146_00385"
     CDS             15229..15726
                     /locus_tag="PK146_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPE90723.1"
                     /translation="MEQLYAPWRMAYIDSADKQEGCIFCDFPKENKDSERLILARGNL
                     VFVILNAFPYNPGHLMVAPYRHEGDYEKLREEEFLEMHRFGQRCVAVLTEAMNPQGFN
                     LGINIGKTAGAGFAGHVHLHIVPRWNGDTNFMPVTAGTKVLPASLHETWDALKKVWEA
                     HARKG"
     gene            15710..16336
                     /locus_tag="PK146_00390"
     CDS             15710..16336
                     /locus_tag="PK146_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPE90724.1"
                     /translation="MPGRDEFYEPASSAEVETRHKRSIFTGRVFRADSEEEARGHIRE
                     ISRRYSDASHNCWAYRVGFPNPVEYYSDAGEPSGTAGKPILGAILRADVVNAVVVVTR
                     YFGGTKLGVRGLIEAYGESATLALEAAGRKGSVRSREAFAECPYEHAQLVLRQMAELG
                     IPEDAVETRWEAAVILSVSVPLSLEDGAASLLGNYTERGFISRWGWKK"
     gene            16355..17860
                     /locus_tag="PK146_00395"
     CDS             16355..17860
                     /locus_tag="PK146_00395"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HPE90725.1"
                     /translation="MRFFDDHSSFCEKLRHPVSRSLLRDVTEDELRAMAAHETVPNNQ
                     GASLYVTKIRSRSAAFTEVLYETDESLVTLLEQVWGYLRWQQMIRVTARIGSPDRQPL
                     FANLYVTRRYARIAQMFAMNFSLELDQDYADIVTVVIPEWHQRKIIVLPRQRVTYILG
                     SDYYGETKMATLRMAMHLGRETMNALGLHAGSKIIRVNTPRGLEEKGVLIFGLSGTGK
                     TTITTADHGLEAPEGVEVLQDDINILLPNGSALGSEANFYIKTDNVTKQQTLLWAARD
                     RKALIENCWVDEDGEIDFDNHAITTNGRAVVPREAIPNTSGRIDLDRVDYLLFNMRRY
                     DTPPIGRLTSAEQAAAYFMLGESTITSAEDPSRVGQAKRVVGFDPFIIDNPHTNGNRL
                     LRILRDNPGIRCYVLNTGRIGGKDGANITAEVTCSAISEAMREKLEWRYDDILGYEIP
                     KELPGQGSAEFDPYRWYSREDYAQMIGSLRRERREHLHQFKGLAPEIVDGV"
     gene            18076..>18417
                     /locus_tag="PK146_00400"
     CDS             18076..>18417
                     /locus_tag="PK146_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPE90726.1"
                     /translation="MRFPVRIMTLSALFAVLTALGAFIRIPFPLVPITLQTFFVFLSG
                     TLLGSRAGALAQVLYVGMGLIGLPVFTGGGGPQYILHPTFGYLLGFIAGAWPIGFISE
                     RSGGPPFALSLT"
BASE COUNT         3844 a         5152 c         5540 g         3881 t
ORIGIN      
        1 agtcatccgc cagcgccttc atctccgaag ccacgcgcag ccggccctcg cggttgaacg
       61 cgtaaccgat ctgctggagg gtcgttccgg taagttcccg gctgaggaac atggccacct
      121 gtcttgcagt ggcaatttcc gaggtcctct tgtttcccga cagggatgat accggtattc
      181 cgaaagattc cgcggtagcc tgctggataa gttctatggt tatctgcccc cttgacgacc
      241 tgcgaataag atctttgagc cacctggatg agttttccac cgtaacggac tcgttgttca
      301 ggtcggcaaa gaacattacg ctgttcaggg agccttcaag ttcccttata ttgctcggta
      361 tgttctgggc caggaaatct atcacgtcgt caggaacgaa gtaatttttg agtgcagcct
      421 tcttcttgag gatagcgata cgcgtttcaa ggtcgggggg ctggatgtcg gtgacgagac
      481 cccactcaaa acggctgacc agtcgctcct ctatgttgtg gatgtccttg gggggtttgt
      541 ctgaggtgag tactatctgc ttcttggcat cgtggaggct gttgaaggtg tggaagaatt
      601 cctcctgggt gctctccttg ttggcaagga agtggatgtc gtcgatcagg agcacatcga
      661 cgctccggta tttcagtttg aactcgtggg tcttgttgtt ctgtatggac atgatgaatt
      721 cgtttatgaa cttttcggag ctaatgtagg cgatcttcgt tccctcattg tgtctggtga
      781 cgaagttccc tatggcgtga aggaggtggg tcttgcccag tccgacaccc ccccagaaga
      841 agagggggtt gtaggccacg ccaggggact cggcaactgc aaggctggcg gcgtgggcca
      901 gcctgtttga ctttcccacc acgaaggagt cgaactggta gtccgggttg aggccgttct
      961 tcggtctctc cgattccctg gagatgcgct cggccctctt gatctcctcc ttctttgctc
     1021 ctcccacctc cagcacgatc ctgtccgcca gccccttctg ggaggtcagg gcggtgagtt
     1081 tttcgatgaa tcgtgactgg atctgttccc gcacgaatac gttggggacg tccagagtaa
     1141 ggacctggtc cacaagggag acgggaagac aggtcttgag ccagatatct gcggcgccct
     1201 taggcaggtt atcctcggct atctcaagga tctttctcca aacctgcgcg gtgttatctt
     1261 ccaagaggtt cacctcgcga ccattttaga tgggggtgag accaatgtta ccatatttga
     1321 atccacaaat tttatccaca tttccacatt ctgttaattt tggagagaaa ggtccgaaaa
     1381 cccccaagga caagggagat tttcgatatg aatggaaata gggtccaatt tgaccaaccg
     1441 taacaaaccc cgttccttag ggaaaaaagt tattcgcatt cctgttcaca tcggacgtgg
     1501 atttctggcc tataatgtcg atagtctatc catgccagcc cgaaaaaagt cctttcaatg
     1561 ggcagaaggg aggtcctgta atgagccgtg ccggtcttca cattatcagt cacaccgatc
     1621 ttgacggtgt aaccgccgca gccgtggcct ggacttacgg ccgccccttg agcggtcctc
     1681 tgagggtgag cctgaccggt tacggggagg tggacctcct ggtcctggat tccctctcga
     1741 ggggcgagcc tctggtcgtg ctcgatctct tttgccagag ggtccagacg gtggatgaga
     1801 tagaccgctc tttttcccac ggcgatcccc ccttcttctt cgaccaccat cagagcacct
     1861 tcgaacgtta cgggagcaga ccctgggttt ttgtagaccg ggagagctgt gccgccatgg
     1921 tctactaccg atggctgatg gataacgccg caagggagga tcgaccccgg ttggtcccac
     1981 tggaggggat agtggaggtc gccaacgatc gcgatctatg gctcaacagg atcccggaga
     2041 gcaggctctg gcaggccctg gtaactctgt gcggtccctg ggccgttctg gcccggctga
     2101 tcgaggaccc ctcaccggag cttagcccgg aggagaggtc cttctcttcc gccttcgtgg
     2161 aggagcagga gaagaggttc agacttgccc tggaaaaggc gttacgatcc ggggaggacc
     2221 tgctctttgt cggccccggt gctctcgagt tcggtgatgt gtcagatttc tgcggtctcg
     2281 tcctcgaccg gatggagtct ccccccaggc ttgcggcagt cctcagcaga cgtcctgcag
     2341 gagactggaa cgtctctatg aggagcaggg agggctttgc cgggagagtg actgggcttc
     2401 tcagggatgg aaagaaggtt cgggggggag ggcacgacga ctcggcggcc ctctactttc
     2461 cccatcatta caggcctgaa gagatacggg attcgctggt ctcggccgtc aggtcggcca
     2521 tcgagtccga cgccccctcg ggtctgtcgc tgggagatct gcttaaacag gcattggagg
     2581 gaaaagagta accgggtcgg gaggtgagcg ggttgtcctt tcgggtggcc attctctacg
     2641 cgtcggtcgg aaccggtcac aggaccgcgg ccatggccct ggagaaatgg ttccaacagg
     2701 agatccctga tccgcaggta ctgtgtctgg acaccctctc cttttcgctt ccaatagtga
     2761 gaggagtcta tacaaggtcc tatctggaga tggtgaggca tatgccccat ctctggggct
     2821 acttctacga gactaccgac gatcccgaaa cgaggaacgg agtaattgcg accctggggg
     2881 agctcactga aaaactgaat atcctgaagc tgaaaaagac acttctctcc ttttctcccg
     2941 acgcgatcgt cttcactcac ttcttcgggg ccgccgccat agcagagtcc ttcgcccccg
     3001 acatccccgt atgctacgtt aacaccgatt tcctgagcca cgtcttccat cgcaatccgg
     3061 ccttctccgc ctggttcgta tccagcgagg aaaccctcaa ccagtatctt gccgacggtc
     3121 tgtcgctgga aagggtcttc ctgacgggca tccccgtcga tcctgcctac gcgtctccac
     3181 cgggcaggga agaggcccgt gaacgtctgg gccttgatat ggacgaaagg atcgccctcg
     3241 tgatgggtgg aggtataggg gtcggtccca tagaagaagt ggtgctttcc ctccacgaag
     3301 gaggtttcgc gacagaagta atctgcggta tcaacgaaaa ggtccggggt gtcctgctgg
     3361 aaaggtttgc cgaccgaccg ggagtgaggg tccacggctt cgtggacaat atgccctgcc
     3421 gctacgcatc ggcggacatc atcttcatga agcccggagg gctttccacc tcggaggctc
     3481 tctgttcagg tacacccatt gttgtcaccg acccgatacc ggggcaggaa cagcgcaaca
     3541 gcgactatct gcttgaccgt ggggcagcaa gggccctctt cgattacagg cttgccgcag
     3601 agaaggccca tagcctgctg tcgacgaaag aagagaaggc ccgcgttctg cgggccgcaa
     3661 aaaggatcgc ccgacccatg gccgggaggg agatcgtcag aaaggtgaag gagataaaag
     3721 atcagtaaaa gacaatgctt ctaccctctt attttgataa ctttttcacc gcctcccgta
     3781 tctcggtaac acctttgtcc agttccccgg aaagtgtctt gagggtcgaa tccagggaga
     3841 cgtcggctgg ccagccagcg gtctcaagga tctcgacggg gtcaagcccc cagacggcgg
     3901 atatctcgcg gatcgtcatg ctggccttga tctcgtccgg attcgacggt gccgtcggag
     3961 acggaatctg tgtcctctcg aggatcctct caagaagcgc ctcgtcgtcg tcgatgcccg
     4021 cgatattctt cagtggaacg tccgtgggga agtcttctgg caggtctacg gcgccgatga
     4081 tctcctgtac ggggaccttc agggtctccg agacgtcctc aagggtcatc catccgtaga
     4141 ggttaggtgc ggccaggcct cccgaagcac ctgaagaggc ggcataggtc tgccaccacc
     4201 ccatccccct ggccagtccg taccctccga aaaagagaga gatcgaaagg atccccagga
     4261 ccagaacgga tatcttgcgg gaggatgtgc cgaagaaaag ggccttctct ctggggcagg
     4321 actccacgca acgtccgcag gcaatgcact ctgcgctcct ctcgacatcc ttcttctcgg
     4381 gatcgagcct tacggggcag gcagggccgc agcgatgaca gtggatgcaa tcatcttcgc
     4441 tccttctgat cttgaagatg ctgatcttct ggagaggggc cagaaaggcc cccagaggac
     4501 agaggtagcg gcaccagaaa cgctcgatga aaagcgacag cccaatgacc aggacgaaga
     4561 gcgaaaagta ggaccagggt gattccacga actcctccca gccggctgac aggtgcatcc
     4621 aggctaccca ggggtcgaag cccctccaga caagtgtccc ggctttccac gttgcgaaaa
     4681 taattactaa cagaacgagg tacttgagcc agcggaggac tccgtcgacc ttcgggggca
     4741 gctcccgctt ctttataccc aggcgggatc ccagccggga ggtccactcc cccagggttc
     4801 ccagggggca gatccatccg cagaagatcc ttccgaacaa cagggtcatg acgaccagga
     4861 ccacgaggag caccatggcg cttggcgcaa gcctcctgag ccatgttccc gaagcgagaa
     4921 tgctgtatag gccctccaga ccgccgaatg ggcagagggc atcaacggtg ggtactcccg
     4981 acgggcctcc tcccagcacc tggtgcctgt agccgagcca ggtcatgaaa cccagaaaac
     5041 cgaactgtac cgaggcgcgg agccatgagg atctcatcct gctgcacacc ttctttcccc
     5101 ccattcaggt ggaaccgggg gcaatatgta caataatact cctgtcaggt atcctctgta
     5161 tcggtggttc tcccgatgtt gcattttccg tgggggacct gccgaaagaa ggagggtgag
     5221 cgtttgtcca tctacctgaa caacggcgct accacatggc ccaagccgcc atgtgtagct
     5281 gaggctgtca gcgccttcat cctcgggagt ggggcgaacc tggcaagggg ttcttcgtcg
     5341 gggcgggata ttgagaccct tgacatggtc acttcgactc gacagcaggt cgcctgtttt
     5401 ttcaggggtt accgaaaaaa cgacccccga tatgtaacct ttacagggaa cgtcaccgaa
     5461 tcgctgaacg ttgtccttaa agggtacctt cgaccaggga tgcacgcctt gacaaccagc
     5521 atggagcata actcggtgat acgacctctg cggcgccttg agggccaggg tgcgaaggtc
     5581 tcgatccttg agtgcgacaa caggggcttt ttgagccctg aagtgctcca ttcgttcctg
     5641 aaaagagaaa gggtcgacct gtttgtgctc tcccatgcga gcaacgtatg cggctccata
     5701 cagcccctgg aggacctggc ggcgatatgc gccggggctg gcgtcccttg cgttctggat
     5761 tcggcccaga cggcaggagt gacagaaata gatgtggagg gactcgggct tgcagcctgc
     5821 tgctttaccg ggcacaaggg tctgatggga ccccagggta tcggcggtat cgtctgggag
     5881 cccggatttg ccggaagggt cctgcccttt gtcgaaggtg ggacggggag tttctccgac
     5941 gacgagcatc agcccgaaaa actccccgac cggttcgagg ccgggactcc caatcttcct
     6001 ggtatcgcag ggctttccgc ctccctggga tggattgctg aaacaggtgt ttcggcaatt
     6061 tttgaaaggg agaaagagct cggcatgagg ttccttgaag ggatccggaa agcgccgggg
     6121 atcaggatct tcggttcgga gaacgagagg agcaggcttg ccgtttttgc ggtgaatctc
     6181 agcagtatgg acaacgggac ggccgccttt gaactctctg aaagatgggg gatcgagacc
     6241 aggccgggcc ttcactgctc ccctcttgga cacaggacgc ttggtacttt cccatccggg
     6301 gcacttcgca taagtatagg gttcttcaac accgaacagg agatagaggt agccattgag
     6361 gccctttgca cccttgcagc tgaaaatgcg cccctttgaa ccgtcagggt ttcaaaaaaa
     6421 agtcgggctc gtgtatcatc aagaggagaa aatttccgga ggtgttgtca atgcagcttg
     6481 atccccagat ctccgacgtc cttcttgccg cccgtttcct gaggggcaag gtgcgtcata
     6541 cccccacgga aaggtcggat agccttagca gcctttccgg cgcagaagtt ttcctcaagt
     6601 gggaaaatca gcagatatgc agttccttca aggttcgtgg ggcgcttaac aagatgtaca
     6661 gcctttcaga ggaagagagg gcgaaggggg ttgtgacggc atccagcggc aatcatgccc
     6721 agggtgtcgc catggcggca agatcccttt ccgccagggc ggcgatatgt gttcccggcg
     6781 tctgccccca gacaaaacgc gacgcgataa tgaggcttgg cggagatttt gtcgaactga
     6841 gggtgatcgg tcatctctac gatgatgctg aggatgaggc ccacaggctt cagaaggaag
     6901 aagggatgac ctacgtctct tcctacgagg atcactttat aatcagcggt gccgggacac
     6961 ttggccttga gatgatcatg gacgaaccct atctcgacac ggtcatagtc cctgcagggg
     7021 gcggggggct gataaacggc gtagccatag cggtaaaggc cttaagcccc gagacggaga
     7081 tctggggggt ccagtctgtg gcatcgaacc cctacgtggt ctcctggccc gggggcaagg
     7141 tgcttgatgt atcctacgag gattccctcg ctgacggact gaccgggtgg ataccccaaa
     7201 gccttcttga cctttcccgg aagagagtta ccaactttgt ggacgtcctg gagtccgata
     7261 tcgcaaaggc catagccttc ctccactccc gtcaccacca ggtggtcgag ggggctgggg
     7321 ctgtcggcgt ggcagccgta atggccggca aggtcccggt gaagggaaag cgggtaggta
     7381 ttgttatatc cggcgggaat atcgatcagc ctcgcctgtt ggagatcctc cgggaatact
     7441 ccgactgaaa ccctgtatgt ttcacgtgaa acaggggccg cccatccagg cggcccctgt
     7501 ttttcattgc tggccagtgc gctgtcgtca ctcgttcccc gggaggatgt tcctcagaag
     7561 ctgttctatg acctgtctct tgatatcttc cccggtatct gggggggttc cggagccttc
     7621 gccggtgggg atgcttatgg tcaccttgtg ctgggtcccg ttctgctggg tttggcctac
     7681 tccgtctgat tccggcaggg gttgtaccgc tccctgagcg ggggcgatcc tgaagctgtg
     7741 gaatgatgga ttggaccagg tcccaccgat acggaagttc gtgtcacgaa agtcctgtct
     7801 tccgagccca cccaccactc cgcctatgag gccctcaaga agggcctcgg gggaggccga
     7861 accggccgtg gcgagaccct gtatggcgcc gagaagggtg ttaagggcct gaacgttgac
     7921 aagccccgag cagtagaggt tcaggttgcc ccccggtcct gcaggtccgt ccacgttaag
     7981 gtaccggtaa agagggtcgt ccacgtaggc tgtggcacga ctgccgggca gaagggtcac
     8041 gacgttcccg tcgattttga agttggcgtc gatcatcctg tagcgaatag aggacatccc
     8101 gtaggcggag gagatcgact tgagggcgtt gaagcccgat atctcccctt ctgtggccga
     8161 gaggttgccc cttcccgtca cgaggacgtg cctcccgaag gctccagaca gggacagatc
     8221 caggtcgccc tttccggaga tgctgccatc caggtcggtg acggccctta ccgtctggtc
     8281 aatgtcggcg cccctgacgg ctgccctgat gttccacctg cctgttgcag tctcgatgga
     8341 gccttcggca ttgatagtcc ctccgtagaa cttcccgacg gcgttctcgg ttctgatgct
     8401 ctcctcctcc aggaccaccg ggagggacgc atcgtcgaag tcaatgccga aggcggtcat
     8461 cctttctgag aagatctccc ccctgccgtt ccatcttccg tctttcatgg agcccgaaag
     8521 ggagacgtcc agagtgcccg acagatccgg ggggccgtta cttttgacct ctttggtaat
     8581 ccgggccagg tcaagacctg atccttccat ggaaaagtcc atatccagac cacccggacc
     8641 aaacagggcc gtcccttttg cagtgaggac tccctcgcca gcggatcctt tcagctcctc
     8701 cagggtcatc tccgagccat tgcccctgac gcggaaagaa atgtccgaca ggttcaggcc
     8761 agcgagtacg atggatggtg actgcccgga aattgccagc tccgggccgg ggaggtcgcc
     8821 cttgatggtg aaactggcct ctacgggccc cttgacgtta aagggaatcc tgttgcccag
     8881 aacggagagg tccacgcctg aagcggtccc cttgaggtcg gttctgcccg gtctgttgga
     8941 cgaggggagc cttgccgttc cggtgacgga gatacgggct cccaggatct ctccctgggc
     9001 gttctcgatg ttcaccgttc cggaggccac ggtgaaagcc gtcctcaggt tcgagagggt
     9061 cccgccgttg aactggagcc tctcggcagc catgtcgagt cgcatgtcgg gagtctcgct
     9121 ggccagaggc agggccactt ttgtaagtat cttcatatcg gtcaggctgg ccttttcggg
     9181 aacgtcgacg cggggcgagc ggatctcaag ggccagcgtg ggagttttta caggtccttt
     9241 aaggttccac tctgccgaga ccctgccctc aagccccagt tctttcaagg ccgggacgaa
     9301 accggccacg gaagatacgg gtccctcctg cagggtcccc gcgaggtcga aggttccctt
     9361 ttcctttacc aggccggtga tccttccgga cccggcaacc ctggaccccc tccacagggc
     9421 gctaatggag ttgatagtga tgttcccttc cgagtaagcg aaactgatct cgggcctttc
     9481 gatggtctcg cccgcagcgt tgatcctggc ggaattgatg gtccccccgg agcgtatctc
     9541 ctgacccgca ccgaagatcc ggaccgagcc ggcggctttc cccgaaaggg caaggttctc
     9601 gacgggaatg accgacgatg cccttgacag gtccaggtcg ttaccggaaa gggtgaggtc
     9661 aaagacaggc ctttgcccga ttgtgatgtc cccttttccg gtcactggag aacccagcca
     9721 gtgagcctta aggtccaggc caattgttga acttttctcg agctggagag tgccgtcgat
     9781 ctccgaaagg ggttgccccg ccagggtaaa atcgggtgtg cttatattta tggtcccaga
     9841 gagagcgttg gagggtccct gaagcctgat ctccatggag tcgatggtcc catcggcaag
     9901 ggagagccat gagaaggatc tcctccagga cttcgcttcg gcctcccggg cggtgatatc
     9961 aatggtggtg accggtggca ttgcactgaa tacgaactga agtttccctg atactgccgt
    10021 cctgttggct tttcccctga tctcccctat ctccagggct cttcctgaga aggaccaggg
    10081 ggaggagatg ccttccatct ctatgccgta gacctcgccc tgggggatgt cgaggtcccc
    10141 tttgactgcg acctcgggcc aggcgcccct ggctgaaaaa gaggttgtaa ggtttccgga
    10201 gtagccctgt ttcgccagat ccggccagaa cctctccagg atcttgatat ccagatcaga
    10261 tacgacgccc gtcaggtctg ccaccgggag gatcctccct gaaacggtca gagcaccacc
    10321 ggtggtcctg atcgacatat ccctgagatc gagccgttct ccggccatct caagctccgc
    10381 cttcagagag aatgtggtct ccctgaaaac gagtgacccc gatgcatcaa ttacaccttc
    10441 ccggaggata actcttccct cttctacctt aatgtccccc gcaggggtcc tgacgatgct
    10501 tctgtggaca cggaacatgt cgaagggcag ggatactgtt cctggagtac cctccttgaa
    10561 caggaggggg aacacttgcg gccattcgag caacgccccg ccgaaggaga tggatctgat
    10621 cctcggagag cctgtcagca gagagagagg cttgaaggat atggccacct caggggctga
    10681 gaagaggagc cgctcgtcac gcctgataga gaggtccctg atcctgaaac ccctgaaggg
    10741 gtttcccgaa atcgaggaga gagaaaggtc ggcctggaga tgctgtacgg cggccttctc
    10801 cagttctgaa gcaacggtcc ctgccccgat atcgcttctg gaagcgagta aaagggctgt
    10861 accgactgcc agcgagagca gtatcaacag gatcagcaga gctttccagt attttcccat
    10921 agagcttacc ccccgagagg accttgcagg acacctaata ataagtacgg aacggaaaag
    10981 aatcaatcgg acaaactacg gaaaactgtg aaggggtttt tggaacttga ttataccaga
    11041 ccgtctcggc ccttcaggtt caaggttgta tgcgcaaggg cagctccggg ggggtatttc
    11101 ctgtggagtt ttgtcaagaa gtgccaaaat gaccggaaag ggggtgtgga gatgtggaca
    11161 aacctgtgga taagtttgga tcagtagaag gataagatct gggtccggtc actcaggata
    11221 acttcgggtc cattaaatcc cggtatgacc ttccctgtta cgatcagggt cctgccggcc
    11281 agatcttcaa tggagccgaa ttgaccggta tcactcttat gaattatcag agtggtatag
    11341 cctttatcag gacgtagcgt gtagcgggtc ctggaagagg aaatgctatc caccttgaac
    11401 cgtatgttga taaaacgccc cgccagcgag ggtaattccg cccatacctg ggttggagtg
    11461 aacagccttc cacgagcctt ttcccagagg cctctcccgg aggacctggc actggcgaag
    11521 gcctccctga aaaggtctgt gtagcgcaga tttgggggga tagcgtaagg gactgccagg
    11581 ccgcttgata ccagcagttc gttggccatg aagcttccct cgtcgctctc tacccagaca
    11641 taggcaaggg tccttccgta gcggtctttc ttctgcaggt caagctcgag aaggaccctt
    11701 cttccgagca ccagcgatct gttgaaacgg gacgcctccc gacccagctc ttccaccctc
    11761 cggcgggggt gagaggtctc ggggcagtct atgccgatat atctcacctg ctccaccttc
    11821 ccgtcggaag tctcaacggt cacggtatcc ccgtcgacga cctcgaccac acgaccccaa
    11881 agagcagtcc ccgccccggc ctgcagaggc gagaaaagga tcagaagaca gcagagagcg
    11941 gcaagacttt tcatttattt tcaggacaga atcttggagg ctctcccttc acaaggacct
    12001 ttctttcgaa ggtcagtccc tccggtacca ggaacaccat ctcgcaggtg ttgttgacag
    12061 tccctccgaa gaggtggcct ccgatgaccg acatgttcct ccccgacagg gaggcgtggg
    12121 catggatgaa tgccttcccg tcgctctttt tgttgaccgt acccgatatg gaaagtatct
    12181 catgggggtc tgagaactga tgtttctcgt atccatcggg gccaagccag cctatctcga
    12241 tatcgcggag catccctatc gcggaaatga tgaccgccga ggtggtgtcg ccaagagctt
    12301 caagaaggga ggggatgagg tcctccccct cgtccagtcg taacgctata aggttcttcc
    12361 ctctttttac tggcaattcg atccctccca ggcagtagat gaagggggca acccctctat
    12421 aagtctatca ccgaaatccc ggtccgaacc cctatgtttt cgggtatgat aataaaatag
    12481 aaaaggaccg tcgatctctt cgaactccgg gaaggatgga ataatacttg ccagagccgt
    12541 ttcccgagca gaggctctca actgaaggac ttctggttga aatggagaag aacagggaag
    12601 ggcttgagag gtacgccctt cttcttgctt ccttcaacga gagagcaagg ctcacaggtc
    12661 ccagagaccc atcggagata ttggggcata tcagggactg cgccgtatct ctggtctttg
    12721 cgccggacat cggcaccgtt gtagatatcg ggacgggtgg aggtcttccg ggtatcgtat
    12781 gggccctttg caggtcgaac ctgaagatca ccctccttga aagcgtgggg aaaaagtgcg
    12841 ctatccttga ggagatggcc ggtatgctca gtctggtcaa cgtcgacgtg gtctgttcaa
    12901 gatcggaggc cctctccctg gagcgcaggg agagatacca aatagccctc tcaagggccg
    12961 taggccacct gggcgtcgtc gccgagtacg cttctcccct gctggcggta ggcggatcgg
    13021 cgtgtctttt caaggggccc agggtcctgc aagagcttag ggagataggg gatagatggg
    13081 ccactctcgg ctttgacgac cccgtgatcc acccttacga tcttgacggg aagagtcctt
    13141 gccttgtgag actgcagaag accggtccgt gcccggcgag gttcccgaga aatcccggca
    13201 aggccaacaa gagcgcctgg tggaggtgaa gttattgaaa acagttgcag ttgcgaacca
    13261 gaagggaggc gtcggcaaga ccacctgctg tgtgaacctg gccgctgaac tgggcagaca
    13321 gggcaagaag gttcttgttc ttgacatcga cccccagggc aacagcacaa gcgggctggg
    13381 tgttgacgta gccaatatca agaacagcat gtatcacgtc atccttgacg aggtctcccc
    13441 caccaatgcg atcatccagt cgtcctggga gggggtctgg gtccttccag ccacccttga
    13501 ccttgccgga tcggaagttg agctcgccgg ggtcatgagc cgggagacaa ggctcgcgag
    13561 gcacatgaac gccatgaagg acttcgacat aggctttatc gactgccctc cctccccggg
    13621 gcttcttacc gtcaacgccc tggtcgcttc cgacagcctt atggtgccga tccagtgcga
    13681 atactacgcc ctggaaggcg tggggcagct catgcggacc gtctcgctgg tgcagagata
    13741 ccttaacgag aacctcaagc ttgacggtgt ggtgctgacc atgttcgaca ccaggaccaa
    13801 cctgtccagg gaagttgctg acgaggtgaa gaaccagttc aaagaggccg tgttcgagac
    13861 cataatccca aggaacgtaa ggctttccga agccccaagc tatgggaagc ctatctgcta
    13921 ctacgatcag ggaagcgcag gcgctgcagc gttcaggaac cttgccgagg aggtgcagga
    13981 ccgatggctc gcccaagggc actagggaaa ggtctcggag ctctcattcc cggagccggg
    14041 gcagatccca tccccgttgc gggggcgcct ggggaggtcc tccatctgcg actgaaggac
    14101 ataaggcctt cgcccaacca gcccaggaag gattttggcc gggaggggct ggaggccctt
    14161 gccgactcca taagggagca cggcatcgtc cagcccgtgg tggtccggaa gcttaacggc
    14221 ggctacgaga tcgtggccgg tgagagaagg tggcgggccg ccgagttggc cgggctcgaa
    14281 gaggtgcccg tccgggtaat agatgcggac gagaacaggg tgatggagct ttccctggtg
    14341 gagaacctcc agagggaaga cctctccccc ctggaggccg ccagggggat ccgggagctg
    14401 gtggagaagt tctcactcac ccaggaacag gcggcgaaga agcttggctg gagcagggtg
    14461 gccgtgtcca acaagctcag gctcctgaac cttcccgagg agatcctgga gatgctgcac
    14521 aggggagatc tcagcgaggg gcacgcgaga gcccttctgt ccctggaaga ccttctggag
    14581 cagatccacc tggcgagaca gtgcgtcgac cgcggcatgt cggtgcggga ggtggaggag
    14641 gcggtccgta agccttcaag gggaaaggat aacagggcaa ggaggtcttc ccagtcggtt
    14701 ttgatcccag agcctgttct cagggtctcg gagcgttacg gcatagacgt aaagatgagc
    14761 ggacgagggg acaacgtaag ggtcatgctg ggcggattga gcgaggatga ggcccgcagg
    14821 ctctttgaga tgatcgaccg gagcggggag atactcttcc ctgacaagtg accctgcagg
    14881 gggtgagtcg atggccaggc tcacggaaga tctaggggca ttcacgaggg tactctcctc
    14941 ggctttactc ctgtgcggat tcatcatctt tggtgtggtt ggcggaaggg ccctggtctt
    15001 gcggggatac cccgggtgga ccctcccggc cgccattatt gccggaacgg ccttcggggc
    15061 atggcaggcc tggtctttcc tgaagacggg gatgaagagg ggccgtaact cgtaacttgc
    15121 cagatgacag tttgctgttt accgtttacc cctttgaatt tttaacagct ccgccgtcgt
    15181 gtttcacgtg aaacgtacat ctcctcaaac tggagagggg ggggtccttt ggaacagctt
    15241 tatgcgccct ggaggatggc ctacattgac tctgccgaca agcaggaggg gtgcatcttc
    15301 tgcgatttcc ccaaggagaa caaagacagt gagcgcctga tactggccag gggtaatctg
    15361 gttttcgtga tactcaacgc cttcccctac aacccgggac atctcatggt ggccccttac
    15421 aggcacgagg gggactacga aaagttgagg gaagaggagt tcctggagat gcaccgtttc
    15481 gggcagaggt gcgtagccgt tctcaccgag gccatgaacc cccaggggtt caacctgggg
    15541 ataaacatcg ggaagaccgc cggggccggt ttcgccggtc acgtccacct ccatattgtc
    15601 ccaagatgga acggcgatac caatttcatg cccgtgacag ccgggaccaa ggtattgccc
    15661 gcctctctcc atgagacctg ggacgctctc aagaaggtct gggaggctca tgccaggaag
    15721 ggatgagttc tacgagccgg cctcttccgc cgaggtcgag accaggcata aacgctccat
    15781 tttcacagga agggtcttcc gggccgattc cgaggaggag gcccgtggcc atatccggga
    15841 gatatccagg cgttactccg atgcgagcca taactgctgg gcctacaggg tgggattccc
    15901 caaccccgtg gagtactact ccgatgccgg agagccctcg gggacagcag ggaaaccgat
    15961 cctgggagcg atcctcagag cggacgtggt caacgccgtt gtggttgtca cccgttattt
    16021 cggcggcacc aagctggggg taagagggct catcgaggcc tatggggaga gcgcgacgct
    16081 ggcactggag gcggcaggcc ggaaggggag cgtaagatca agagaggcct tcgcggagtg
    16141 tccctacgag cacgcccagc tggtcctccg gcagatggcg gagctcggga tacccgagga
    16201 tgccgtcgaa accaggtggg aagccgccgt gatcctttcc gtatcggtgc ccctgtccct
    16261 ggaggacggc gcagcttctc tcctcgggaa ctacaccgaa agaggcttca tctcccgttg
    16321 gggatggaaa aaatagacct ggaggcgatc ggaagtgcga tttttcgacg accattcctc
    16381 cttctgcgag aagctccgcc acccagtgag caggagcctt ctccgcgacg tcaccgagga
    16441 tgaactgaga gctatggccg cccacgagac ggtgcccaac aaccaggggg cctccctcta
    16501 tgttacgaag atccgctccc ggagcgccgc tttcaccgaa gtgctctacg agacggacga
    16561 gtccctggta acgctcctgg aacaggtttg gggttacctt aggtggcagc agatgatacg
    16621 cgtaaccgcg aggataggct ccccggaccg tcagcccctt ttcgcgaacc tctacgtgac
    16681 gcgaaggtac gcgaggatag cccagatgtt cgccatgaac ttctccttgg aactggacca
    16741 ggactacgca gacattgtca ccgtggtgat cccggaatgg catcaacgta agatcatagt
    16801 cctgcccaga cagagggtca cctatatcct gggaagcgac tactacgggg agaccaagat
    16861 ggccaccctg aggatggcca tgcacctggg gcgcgagacc atgaacgccc tgggccttca
    16921 cgcaggaagc aagatcataa gggttaatac tcccaggggg cttgaagaga agggtgtgct
    16981 gatcttcggg ctatccggga ctggcaagac caccattacc accgccgacc acggcctcga
    17041 ggccccggag ggcgtggagg tgctccagga cgatatcaac atccttctgc ctaacggaag
    17101 cgccctgggt tcggaagcga acttctacat caagaccgac aacgtgacga agcagcagac
    17161 ccttctgtgg gctgcgcgcg acagaaaggc tcttatcgag aactgctggg tggacgaaga
    17221 cggggagatc gacttcgaca accacgccat aactaccaac ggaagggccg tcgtgcccag
    17281 ggaggccata ccaaacactt cggggcgcat agatctggac agggtagact acctgctctt
    17341 caacatgagg aggtacgaca ctccgcccat cgggcggctc acatcggcgg agcaggccgc
    17401 agcctatttc atgctgggag agtctacgat aaccagcgcg gaggatccct ccagagtcgg
    17461 acaggccaag agggtggtgg gcttcgatcc cttcattatc gacaaccccc acacaaacgg
    17521 gaacaggctt ttgaggatcc tcagggacaa ccccgggatc aggtgctacg tccttaacac
    17581 ggggaggata ggtggaaagg acggggctaa cataaccgcc gaagtcacct gttcggccat
    17641 cagcgaggcc atgagggaga aactggagtg gcgctacgac gatatccttg gttacgaaat
    17701 accgaaagag ctgcctgggc agggatcggc agaatttgac ccctacaggt ggtactccag
    17761 ggaggattac gctcaaatga tcgggagcct tcgccgggag aggagggagc atctccatca
    17821 gttcaagggc cttgctcccg agatcgtgga tggggtttag cggcaagaag cgtgagatgt
    17881 gacgaataag cgaaaggcaa ggcggcaggg gacagggtta aggaggcagg aagggcaggg
    17941 ttcgcagttg atcctggatt cacttttcac gagtttttgc ctctccttct tccttgcctt
    18001 ttcacttccc ctgctgtagt attcctccat agtatgtcaa cctattaaat ttttggttga
    18061 cagtggaggt aggccatgcg tttccccgtg aggatcatga ccctttcggc ccttttcgcg
    18121 gtactgaccg ccctcggggc cttcatcagg atacccttcc cccttgtccc catcacgctc
    18181 cagaccttct tcgtgttcct ctccgggacc cttctggggt cacgcgcagg ggccctggcc
    18241 caggtcctgt atgtcggcat ggggcttata ggccttcccg tgttcaccgg gggggggggt
    18301 ccccagtaca ttctccatcc caccttcggc tatctgctgg gctttatcgc cggggcctgg
    18361 cccatcggtt tcatatccga aaggtccggg gggcccccct tcgccctgtc cctgacg
//
LOCUS       DAOPKQ010000005        17183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_3515_length_17183_cov_5.391114, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000005 DAOPKQ010000000
VERSION     DAOPKQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3515_length_17183_cov_5.391114"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PK146_00405"
     CDS             complement(<1..1650)
                     /locus_tag="PK146_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE90727.1"
                     /translation="MRKRDLLLCCIVVLALCLLLPPGPAWAFHHVKAGFCQNKPLVFR
                     GADGVVKGIYADFLNAVAVENEWTLEWVEGTFAEGLERLERREIDIMTAVEWTEQRQE
                     RYDFTRKAVISNWGVFYSRRGMTAESIPDLEGMRIAVVRRDAYAAAFRELADKFGITC
                     SYLEVDDYPDVLKAIEEWRTEAGLVSRLFGLANESDYHVDRTNLFIEPMELRFAAPKG
                     FSVTILQILDFHLTKWRTIPNSPLNTSIQNWLPGEHPIGVIPSWFPWFVGASLALVLG
                     VLFANMMLRRQVLSRTRELLKSRVDLSREKVFFEKLFEESPDALVLESKEGNRILRVN
                     SGFTRLFGYIREEVEGKTLNDVVVPEELLQEGMALDVETAQGNPILVETVRKNKDGKP
                     IDVSLISVPVTLEEGVIASYTIYRDISAAKEARRALIESRESIRKSLESMRRTWEQTI
                     EVLATAAETRDPYTAGHQRNVSVLSEAIATELGLDEDKVNSVRMAGLVHDIGKINVPA
                     EILSKPGTLGEVEFALIMSQQETAYEIMKNIELPWNLADMIL"
     gene            complement(1771..3057)
                     /gene="rmuC"
                     /locus_tag="PK146_00410"
     CDS             complement(1771..3057)
                     /gene="rmuC"
                     /locus_tag="PK146_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPE90728.1"
                     /translation="MKKNEGDDDVMASLAPLAPLLAVFLAGFLFGILAALILRVIHGR
                     TARDLAEELFRENEAERRDQIEEVTEHIKAVFGDLSLEALSRSTEEFLKLAKSRFEAE
                     RELGDRELERKKELIDRRLEEMVTKLEAVSSLMRELEKDRASKFGELADRLREVNERT
                     ADLNRTTGTLKEALSSSKARGQWGERMAEDILRMVGFEEGVNYARQRTLPGSGRRPDF
                     TFLLPRGLTLNMDVKFPFDNYLAWLGAANDTERDKFRRSFLKDVRDRIREVASRDYID
                     PGQNTLDYALLFIPNEQVYSFIHEQEPGMVDEGLRQRVVFCSPVTLYAVLAVIRQAVD
                     NFTLEQASKEMLGLFGAFSKQWKEFLKKLEHLGKQLEGAREDYESLINTRRRALDRPL
                     ERLEEIRKLGGIGTASMEETLPECSHNGDDGRISTE"
     gene            3124..3702
                     /locus_tag="PK146_00415"
     CDS             3124..3702
                     /locus_tag="PK146_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPE90729.1"
                     /translation="MRVLAFNASPRADGNTALLLGRVLSVIQKEGIDTELVHIGGRSI
                     RGCTACGGCSRTLDDRCSLPDDGINEWIPRIREADGIILGSPTYFADVTAEMKAFIDR
                     VGYVSRANGGLLRRKAGAAVIAVRRCGSIHAFDTINHFFLINEMVVPGSSYWNMAIGR
                     GPGEVAGDEEGIRTMENLGKNMAWILSRISDR"
     gene            3789..7544
                     /locus_tag="PK146_00420"
     CDS             3789..7544
                     /locus_tag="PK146_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE90730.1"
                     /translation="MPLSDDSLRTNLEIVGDHLHLLVESSPEGMVICDDSNRIIFANP
                     QFCNMFGWERDEVTGKDIDEVVAGAEEVAAEASLVSSTVFSTPHMIPQAVRVRKDGKR
                     INVSILAGPVLDNGRTVGAYGIYRDISFKKKAEDDIRREKAFFENLFQKAPEAILLCG
                     REARGIKVNRAFTNLFGYTQEDIEGKDINPLIAKEPELLREARGLDALSWSSGKPFSL
                     DTWRTRKDGTRIPVGILQFPFKVDDENEVEYTIYRDRTSELRAKAALRREKAFFENLF
                     EACPDAIILSNREGEVLRANRAFYELFGYSEEDRVIGGDIFSIVARNPRSKKEARELE
                     ERFWGGEEIDREVVRQRKDGSDIHLHLTQVPFRLEEGNVVDYIIYRDIEHRKKAERET
                     LRHLAFERLISSISSLFLSPGEITEQAREAVSEIAVHIGATHAFVNLFSDGEEVTPLA
                     RWASPDMEPVNSPHINQPWTWLAERIRKEPVVYVPNTEALPPEASGEYSFALSLGWKS
                     FVAYPVFAGDRCRGFALFGDSLREDPWRGPDAPAGKIFCNIIGQAILRHDLEEARRES
                     ERFLNDILQFQTDFVVRIKPDLSVIFANRAFSEFLGLPEGLPRGSSLKGLMIEEDLEQ
                     GLRMVEPLSRENPVSVSEEEMTGHDGSKRWIQWIDRGIFDGSGNLVEIQGVGRDISRL
                     RRTQEALKESEQKFLNIVEDQDEFIIRFKPDGTLTFANSSYCRRFGKKLEEILGQCIF
                     DHVDQDEASRLKREHFDLLTPENPTNTDESWSLLPDGQTRLEQWHNRGIFDQEGNLVT
                     IQCVGRDITPQREAEQALRESERRYAAIVESQVEMVCRFNPEGTITFVNDAFCLFFGL
                     EREHILYRPLSEVIEDAGASSPITRHDDLSPSDPASFFEHSLTLPTGKKRWLRWSRRG
                     IFDEKGDLREIQAVARDMTDMKRTEEELRHLNSILRSIRGVNRLISRHQESERLVQEI
                     CAHLVETRGYRNVWIGILGRGGSLASVSEAGIGSDGRPMSERIRHGMLNEWGMSVLKE
                     PGIHMKRGIMTMTDCPLLDKYEEAGIMTTRLEFAGKTYGLISVSITGEAPIDPEEQAL
                     FLELAEDISFALHVIETEMDRREKATTLEEKARQLELLVSEHPSGLWEWDVSEGRVVM
                     NSAYETMLGYSPGEMGQSIENWKGQIHPDDLPGMIDLLNRAISGGNGENIYEADYRML
                     KKEGTWLTLRSRGFVVRRDRNGRAARMVGIHTPICQDHQAGKEEERR"
     gene            7541..8014
                     /locus_tag="PK146_00425"
     CDS             7541..8014
                     /locus_tag="PK146_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPE90731.1"
                     /translation="MRRKDKEITEAAQIRGILERGKVCRIAFFDDEFPYMVPVCFGFD
                     GRSIFFHGFNGGKKMECLRKDPRVCFEVEEDIEVVSDPDPCEWTLKYRSVVGFGKATV
                     LEGSEEKRAGLDVVVRQFAGRELSFPDQAMAKVAVVRIDIESMTGRRNGYPPREN"
     gene            8017..8640
                     /locus_tag="PK146_00430"
     CDS             8017..8640
                     /locus_tag="PK146_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPE90732.1"
                     /translation="MEAFITAAISWIVSTIGRMGYTGVVALMFLESSFFPFPSEVVVP
                     PAGYLASQGQMNLFLVIAAGIAGSILGALFNYWISLTFGRPFFRRFGKYVFVGEKALD
                     RADEFFAKHGHISTFIGRLLPGIRQLISLPAGLARMNIPLFLFFTTLGSGIWVVILAY
                     VGYWVGNNQDLVHQYMHTATYISIAACALLAIGYVIHYKRKNKKRNA"
     gene            9304..10932
                     /locus_tag="PK146_00435"
     CDS             9304..10932
                     /locus_tag="PK146_00435"
                     /EC_number="4.1.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF005712.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-guanidino-2-oxopentanoate decarboxylase"
                     /protein_id="HPE90733.1"
                     /translation="MSSPNGARRVVNTLESLGVFVIFGIPGIHNLDIYTALTESEIRH
                     VTARHEQGAGFMADGYARSTGKTGVALVISGPGLTNILTPMAQALHDSIPMLVISSQI
                     PTPFIGRRCGFLHELPDSASLAQGVSKKSFCARTPEEVPAVIRSAWDLASDGRPGPVH
                     VEIPMDVLGQTSGGKVSFQDSPPPYPDFLIPEGAMTRTADLLDNSSNPVIFVGGGSCG
                     ASEALSKLAEISGAMVIETCAGKGTVDERNPLCLGARSHFPCVREALSEADLILAFGT
                     ELSPTDLWEKPLPKNVTLVQVDLDPANFSRNGMADIGITADANEIASALLQMVSPNPS
                     RSAGTKEQVSRLIEKSSEELATVTGMGDELDSMNALVSAIRKAIPEEGILFADMTGPA
                     YVAISEYPAYSPRTFLHPVGFGTLGLALPGAIGAAIARPSRPVCVMAGDGGFQFTLPE
                     LAVAVQERLPIPVVIWNDHGFGEIRRNENARHPGMFIAVDNTPPDLALLASAYGAKYS
                     RPETPGDISASIRQAFSAGVPTIIECTTGNGGVH"
     gene            10932..12089
                     /gene="hisC"
                     /locus_tag="PK146_00440"
     CDS             10932..12089
                     /gene="hisC"
                     /locus_tag="PK146_00440"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPE90734.1"
                     /translation="MTVKGLRPEVLSMETYVPGKTIEEVRRELGLERVVKLGSNENPY
                     GPFPKALEAMMKELAEANRYPDIAFEEIKGLLAKLHGVTQKNIAISHGAEGMLQAMGK
                     CFISPGDEVLIPSATYGLYREISRLMGAKVVNIPMNGYSTDMKGIANAVTDRTKLVWI
                     CNPNNPTGTVIDPESLEKFFMALPEDAWVVLDEAYAEFASPEELPDRARLISEGKNLV
                     SVRTFSKAYGLAGARLGYGMAREKVVTAIDTVSEPFNANRLGIAAGVAVLTRDREACE
                     RARALILTERERVSQHLQAMGMSVVPSRTNFIFFDTPFHAENLAEELLYRGVIVRPCG
                     GWGYQGAIRVTVGTEEENDIFLAALKETVETMREKDSGTGSHREVEDGPVR"
     gene            12073..13206
                     /locus_tag="PK146_00445"
     CDS             12073..13206
                     /locus_tag="PK146_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163919.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HPE90735.1"
                     /translation="MVLFDSVTKVYEDGTRAVDGLDLEIGRGEIAVLIGPSGCGKTTT
                     LKMVNRLEELSSGRILVDGKDINSLDPVQLRRSIGYVVQEIALMPHMSVAENIAIVPR
                     LLGWDKKRIKKRVDELLELARLEPSKYRYRLPDQLSGGQKQRIGVLRALAADPEVILM
                     DEPFGALDPISREGLQNELLELQNAVKKTIVFVTHDMEEALRMADQVVIMRKGRIEQM
                     GTPEEIQDRPATEFIRNFIGEDRLSNISPESPVEILVQDPVIKARSDSMASELLDLME
                     EEGCETAQVVDKKGKWRGMALLWLIKRAARSGLPASDGITRDRKLQAGDSTLRDAAEM
                     LADQDLPIPVISDTGDLLGVVTQAGIARLTIGRLTRKRREVRE"
     gene            13206..13904
                     /locus_tag="PK146_00450"
     CDS             13206..13904
                     /locus_tag="PK146_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE90736.1"
                     /translation="MFQQRPFWELFSAYVIRFWPRILQLTWQHIVISSIALAITLVVC
                     IPLGIYLTKNEKLAPYAIGVANIFQTIPSLALLGFLIFIFGIGNDNAICALFLYAMLP
                     VIQNTYTGLKNVPRHLILAARGMGMTEYQILTRVQLPIGLPVILAGVRVATVWIIGTA
                     TLASAIGGGGLGRLIFSGLASIRNEVVLAGALPATLLALGADQLLKLFQAYSDPKKRA
                     MRISKRSGPSGDRP"
     gene            13944..14897
                     /locus_tag="PK146_00455"
     CDS             13944..14897
                     /locus_tag="PK146_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HPE90737.1"
                     /translation="MKGKTMLLMVVAAFVIVVGLVVMNNGKQEVPPPPEEAIVAETDP
                     ADYEGTVRIGGQTVNESIVLAWMGKLLIENHTGLKADINTEFAASSVLHQAMSGGELD
                     IYPTWTGTQLTGVLRYEGPNLPSDETFQMVKEGFEENFNMTWTSPIGFNNTYVMAVRR
                     ETAEKLELEKASDLADHASGMRLAGDENFDTRPDAYPGWSEAYGIEFGEVLPMQYSIM
                     YRAIANEEVDVIAAYSTDSRIKKLDLALLEDDMSFFPDYSAAFVVRMEILEEYPELLD
                     VLEKLSGTIDEVTMSELNGRFDDGEEPEDIAKDFLSGAGLI"
     gene            15029..>17183
                     /locus_tag="PK146_00460"
     CDS             15029..>17183
                     /locus_tag="PK146_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPE90738.1"
                     /translation="MSMRDIFQGSPFGGGGGEPRRQIRPLRFRKSVWILFGIVLLLAV
                     VIPATATFYTDMLWFRERGLSQVFWTRLIPQWILFAVAAVAAFAIFSLNWLKARRSAI
                     KDLASSLPEEAGDMPLRASAVVVAIIAGALAVMNGLGIRSEWMTVLQFFNRTPFGKND
                     PLFGKDIAFYVFEIPFLSMLQGWLLNTLIMALMGVALIVFLAAFPKMREENRIYLPSH
                     ARSHLSILGAVTVLVWGAGMWLERFNLLLSPEGVVFGAGYTDVHVRLFAINVMIALSV
                     IVAALLVANLYKRTWRLAIAGGILLVGASFLLRGVVPGIIQKYVVEPNEFSKERPYIE
                     FNINSTLEAYGLDSLEIIDFSPENAITRENIDNEPDTIRNIRLWDYRPLLRAFKQLQE
                     IRTYYDFPDVDIARYTFDGSYRQVMLAARELDHGQLQNPTWVNRHLEFTHGYGIVMNF
                     VNEVDSAGKPVLIVKDLPPQMSVPLRIEKPQIYYGEKTNPYSLVRTSILEFDYPMGDA
                     NVRTTYEGTGGVPIGNLFRRIMFALRYRDSEILFTNVITSESRVLMYRSIRERLDKVA
                     PFLLYDSDPYLAIIDGRLVWIQDAYTATDRYPYSNPVSVTGTLQRINYIRNSIKAVVD
                     AYDGSILLYISDGEDPMIRTWAGIFPALFTPIEDAHPELCTQIRYPMAQFLIQSEIFR
                     TYHMSDPNTFYNKEDVWRLDNQTTTGSLNAYYLIMR"
BASE COUNT         3847 a         4945 c         4881 g         3510 t
ORIGIN      
        1 gaggatcatg tctgccaggt tccatggcag ttcgatgttc ttcattatct cgtatgccgt
       61 ctcctgctgg gacatgataa gagcgaactc gacctccccg agagtccctg gtttgctcag
      121 tatctccgca gggacgttta tctttccaat atcgtggact agtcccgcca tcctgacgga
      181 gttgacctta tcttcatcca ggccaagttc tgttgcaatg gcttcagaaa ggaccgatac
      241 gttcctctga tgaccggcgg tataagggtc ccgcgtctcg gcagcggtcg caaggacctc
      301 aatggtctgc tcccaggttc gccgcatgct ctctaggctc ttgcggatac tctcccggct
      361 ttctatgagt gcccttcggg cttccttggc agccgatatg tccctgtaga tcgtatagga
      421 agctattacg ccctcctcca gggtcactgg tacggagatg agggacacat cgatgggctt
      481 cccgtccttg ttcttcctga ccgtctccac cagaatgggg ttaccctggg cggtctcgac
      541 gtcaagagcc ataccctcct ggagcagttc ttcagggact accacgtcgt taagggtctt
      601 gccctccacc tcttcccgga tgtagccgaa aagcctggtg aaaccactgt tgaccctgag
      661 gatcctgttg ccctccttgc tctccagcac aagggcatcg ggggactcct caaagagctt
      721 ctcgaagaag accttctccc gagaaaggtc caccctgctc ttgagcagct ccctggtcct
      781 ggacaagacc tgacgcctca acatcatgtt ggcgaagaga acgcccagca cgagggccag
      841 tgacgccccc acaaaccatg ggaaccaact ggggatcaca ccgatggggt gttcccctgg
      901 aagccagttc tggatagacg tgttaagagg ggagtttggg atcgtcctcc acttggtgag
      961 atggaaatcc aggatctgga ggatagtaac actgaatccc ttgggggctg cgaacctcag
     1021 ctccatgggt tcaatgaaga ggttggtcct gtccacatga tagtcgctct cattggccag
     1081 accgaagaga cggcttacca gaccggcttc ggtcctccac tcctcgatgg cttttaatac
     1141 atcagggtag tcgtccacct caagatagga gcatgtaatc ccgaacttgt cggccagctc
     1201 ccggaaggcc gctgcgtagg cgtcccgtct tactacggct atcctcatgc cttccagatc
     1261 ggggatcgat tctgcggtca tgccccggcg agagtagaat accccccagt tggagataac
     1321 cgccttacgg gtgaaatcgt accgctcctg tctctgctcc gtccattcga cggccgtcat
     1381 gatgtcgatc tcgcgccgtt caagcctctc gagcccctca gcgaaggtcc cctccaccca
     1441 ctccagggtc cattcgttct ctacggccac agcgttgaga aagtcggcgt agataccctt
     1501 gaccacaccg tcggctcctc tgaataccag gggtttgttc tggcagaaac cagccttcac
     1561 gtgatggaac gcccaggcgg gacccggtgg gaggagaaga cagagggcaa gcaccactat
     1621 gcagcagaga aggagatccc tttttcgcat gcggtgcacc ccctttcaga tcgaccgaga
     1681 acgttccgag gcaggatctg cagattgtat catctattat tggttacaca tttcggattg
     1741 tatcatctat tagaaggtca gagcaacgat tcattcagtt gaaatccggc catcgtcacc
     1801 gttatggctg cattcgggga gagtttcctc catggaggcg gtgccaatac cgcccagctt
     1861 ccggatctcc tccagtctct ccagggggcg atccagtgcc ctgcggcggg tgttgataag
     1921 actctcatag tcttctcggg ccccttcgag ctgcttcccc agatgctcca gtttcttgag
     1981 gaactctttc cactgcttgc tgaaggctcc gaagagaccc aacatctcct tcgaggcctg
     2041 ctccagggtg aagttgtcca cggcctgcct gatcacggca aggacagcat aaagcgttac
     2101 gggggaacag aagaccactc tctgcctgag cccttcatcg accatgcccg gctcctgttc
     2161 gtggatgaag gagtacacct gttcgttggg tatgaataga agggcatagt cgagggtgtt
     2221 ctgtccgggg tctatataat ccctcgaggc aacctccctg atcctgtccc tcacatcctt
     2281 caggaacgat ctgcggaact tatctctttc agtatcgttt gcggcgccaa gccaggcaag
     2341 atagttatca aaggggaact ttacatccat gttgagagta agccctctgg gtagcaagaa
     2401 ggtgaagtcg ggcctccgcc cggacccggg cagggtcctc tgtctggcgt aattaacacc
     2461 ctcttcaaaa ccgaccatcc ggaggatatc ctcagccatc ctttcacccc actgtcccct
     2521 ggccttggag ctcgaaagag cctccttgag ggttcccgtt gtcctgttca gatcggctgt
     2581 cctctcgttg acctccctaa ggcggtccgc aagctctccg aacttggatg cccggtcctt
     2641 ctcgagctcc ctcatcaggg aagagacggc ttccagcttt gtcaccatct cctcgagtct
     2701 gcgatctatg agctccttct ttcgttccag ctcccggtca cccagttccc tctccgcctc
     2761 gaagcgtgac ttggcaagct tcaggaattc ctccgtggac cttgacagtg cttccagaga
     2821 gaggtcgccg aagacggcct tgatgtgctc cgtgacttcc tcgatctggt ccctgcgctc
     2881 ggcctcgttc tcacggaaaa gctcctcggc aaggtccctg gcggtgcgtc catggatgac
     2941 cctcaggata agagcggcga ggatgccgaa gaggaatccc gccaggaaaa ccgccaaaag
     3001 gggagctaga ggagcaaggg aagccattac atcatcatct ccctcgtttt ttttcattat
     3061 atacgccccg gtactgctaa aatcacttca tacccaacta ccacggaaag gagtgtcctg
     3121 atcatgcgtg tcctggcctt caacgccagc cccagggccg acggaaacac ggcgctgctt
     3181 ctgggcaggg tcctgtctgt gatccaaaag gaagggatcg atacagaact ggtacatatc
     3241 ggtgggcggt caataagggg gtgcaccgcc tgcggcggtt gttcccggac cctcgatgac
     3301 aggtgcagtc tccccgacga cggtatcaac gaatggatac caaggatcag ggaggccgac
     3361 ggtatcatcc tcggatcacc tacctacttc gccgatgtca ctgctgagat gaaggctttt
     3421 atcgacaggg tgggctacgt atcgagggcg aacgggggcc tactccgccg gaaagcgggg
     3481 gcggcagtta tcgccgtgag gcggtgcggt tcgatccacg cctttgacac catcaaccac
     3541 ttcttcctta tcaacgagat ggtggtcccc ggttcaagtt actggaacat ggccatcggg
     3601 agggggccgg gcgaggtagc cggtgacgaa gaggggatca ggactatgga gaacctgggc
     3661 aagaacatgg cctggatcct ttccaggatc tccgataggt gaaagtctgc gtccatgcct
     3721 ggacgtctta cggaccatga tagaatattt tctgcccccc ggggtaaacc ctttcggagg
     3781 tggaccgatt gcccttgtcc gatgacagtt tgaggacaaa ccttgagata gtcggggacc
     3841 atcttcacct ccttgtggag agctctcccg agggaatggt catctgcgat gacagcaacc
     3901 ggatcatctt cgccaacccc cagttctgca acatgttcgg ctgggagagg gacgaagtga
     3961 cgggcaagga tatcgacgag gtggtggcag gagcggaaga ggtagcagcc gaggcatccc
     4021 ttgtttcgtc aaccgtcttc tcgaccccgc acatgatccc ccaggcggtc agagtgagaa
     4081 aggacggcaa aaggataaac gtatccatcc ttgccgggcc tgttctcgac aatggacgga
     4141 cagtcggggc ctacgggatc taccgtgaca tctcctttaa aaagaaggcc gaggatgaca
     4201 taaggagaga gaaggccttc ttcgagaacc ttttccagaa ggccccggag gccatcctcc
     4261 tctgcggaag agaagcaagg gggatcaagg tcaacagggc ctttacgaac cttttcggct
     4321 acacccagga agacatcgag ggaaaggaca tcaaccccct catagccaaa gaacccgagc
     4381 tgctcagaga ggcccgtggc ctggacgctc tcagctggag ctccgggaag cctttcagcc
     4441 tggacacctg gaggaccagg aaggacggaa caaggatccc tgtggggatc ctccagttcc
     4501 ccttcaaggt tgacgatgag aacgaggtgg agtacaccat ctaccgggac aggacctccg
     4561 aactcagggc caaggctgcc ctgaggaggg agaaggcctt cttcgagaac ctcttcgagg
     4621 cctgccccga cgcgataatc cttagcaaca gggagggaga ggtcctccgg gccaacaggg
     4681 ctttctatga actattcggc tactcggaag aagaccgggt tatcggcggt gatattttca
     4741 gcatcgtcgc caggaacccc aggagcaaga aggaggcccg ggaactggag gaacgattct
     4801 ggggcggcga ggagatcgac cgcgaggtgg ttcgccagag aaaggacggc tccgatattc
     4861 acctccacct tacccaggtc cccttccgcc ttgaggaggg aaacgtagtt gactacatca
     4921 tctacaggga tatcgaacac aggaaaaagg cagaaaggga aaccctccgg cacctggcct
     4981 tcgaaaggct tatctcttca atatcttccc tgttcctttc acccggggaa ataaccgaac
     5041 aggcccggga ggccgtctcg gagatcgcgg tccacatagg ggcgacccac gccttcgtta
     5101 acctcttctc cgacggggag gaagtaacgc ctcttgcccg atgggcctcc cctgatatgg
     5161 agccggtaaa cagccctcac attaaccagc cgtggacctg gctagcggaa aggatcagaa
     5221 aggaacccgt cgtctacgtc cccaatacgg aagcattgcc cccggaggca tctggagagt
     5281 attccttcgc cctctccctg ggatggaagt ccttcgtggc ctatccagta tttgccgggg
     5341 atcgctgcag gggtttcgct ctcttcggcg attccctcag ggaggatccc tggagagggc
     5401 ccgacgcccc ggccgggaag atcttctgca acatcatcgg ccaggccatc ctgaggcacg
     5461 acctggagga ggcccgccgt gaatcagaac gtttcctgaa tgacatactc cagttccaga
     5521 ccgattttgt ggtcaggatc aagcccgact tatcggtcat attcgccaac agggctttct
     5581 cggaattcct tggcttgcct gagggccttc cgaggggctc atccctgaag ggtcttatga
     5641 tagaagagga cctggaacag ggactcagga tggtggaacc cctttcacgg gagaaccccg
     5701 tatcggttag cgaagaggag atgacagggc acgacgggtc gaaaagatgg atccagtgga
     5761 tagacagggg catcttcgac ggaagcggca accttgttga gatccaggga gttggaaggg
     5821 acataagccg attgagaagg acccaggaag ccctcaagga gagcgaacag aagttcctga
     5881 acatcgtgga ggaccaggac gagttcataa tccgcttcaa acccgacggg accctcacct
     5941 tcgcgaactc ctcctactgc aggcgcttcg gcaaaaaact ggaggagatc ctggggcagt
     6001 gcatcttcga ccatgtggac caggatgagg catcacggct taaacgggag cacttcgacc
     6061 ttctcacccc cgaaaacccc accaacacag acgagtcgtg gtcgttgctc cccgacggac
     6121 agacgcggct cgagcagtgg cataacaggg gcattttcga tcaagaaggc aacctcgtaa
     6181 ccattcagtg cgtgggcagg gatatcacgc cccagagaga ggcggagcaa gctctccgcg
     6241 agagtgagag gcgctacgcc gccattgtcg aaagccaggt cgagatggtg tgccgcttca
     6301 atcccgaagg aacgatcacc ttcgtgaacg acgccttctg cctcttcttc gggctcgaga
     6361 gggaacacat cctttaccgc cccctttcgg aggtcatcga ggatgccgga gcttcgtcac
     6421 ccataacccg ccacgatgat ctctccccat cggaccccgc ttccttcttc gaacacagtc
     6481 tgacccttcc cacggggaag aagcgctggc tgagatggtc aagaaggggc atcttcgacg
     6541 aaaagggaga cctgagggag atacaggcag tagccagaga tatgaccgac atgaaaagga
     6601 ccgaggagga gttgagacat ctaaactcga tcctccgttc catcaggggg gtcaaccggc
     6661 tgatcagcag gcaccaggaa agcgagaggc tcgtccagga gatatgtgcc caccttgttg
     6721 agacaagggg ttaccggaac gtgtggatag gtatccttgg aaggggcggc agcctggcat
     6781 ctgtatctga agcgggaata ggctctgacg gaaggcccat gtcggagagg atcagacatg
     6841 ggatgctcaa cgaatggggg atgtccgtcc ttaaggagcc ggggatccac atgaagcgcg
     6901 gcatcatgac catgaccgac tgccctctcc tcgacaagta tgaagaagcc ggaataatga
     6961 ccacccggct tgaattcgcc ggaaagacct acggtctgat ctcggtatcc attaccgggg
     7021 aagctcccat cgaccccgag gaacaggctc tcttcctgga gctcgctgag gatatctcct
     7081 ttgctctcca cgttatcgag accgagatgg accgccggga gaaggccacc acccttgagg
     7141 agaaggcccg gcaactcgag ctgctcgtct ctgagcaccc ctccgggctt tgggaatggg
     7201 acgttagcga ggggagggtc gtcatgaact ccgcctacga aaccatgctg ggctactccc
     7261 ccggcgagat ggggcagtcc atcgaaaact ggaagggaca gatccacccc gatgacctgc
     7321 cagggatgat tgaccttttg aacagggcca tctccggcgg gaatggggag aacatctacg
     7381 aggccgacta tagaatgctc aagaaggaag ggacctggct gacgctccgg agccgggggt
     7441 ttgtggtccg cagggaccgc aacggaaggg ccgccaggat ggttgggatc cacaccccga
     7501 tctgtcagga ccatcaggca gggaaagagg aggagagacg atgaggcgaa aggacaagga
     7561 gataaccgaa gctgcacaga tcagggggat cctggagagg ggcaaggtct gcaggatcgc
     7621 attctttgac gatgagttcc cctatatggt tcccgtgtgt ttcggcttcg acgggagatc
     7681 catctttttc cacggcttca acggcggcaa aaagatggaa tgcctcagga aagacccccg
     7741 ggtctgcttc gaggtcgaag aggacatcga ggtagtctca gatcccgacc cgtgtgaatg
     7801 gaccctcaag tacaggagcg tagtgggttt cggtaaggcc accgtactgg aaggctccga
     7861 agaaaaaagg gcagggcttg acgtggtcgt ccgacagttc gcgggacggg agctgtcatt
     7921 tcccgaccag gccatggcca aagtcgccgt agtcaggata gatatcgagt caatgacggg
     7981 acggcgcaac ggctatcctc cccgggaaaa ctgaaaatgg aagcttttat cacggcggcg
     8041 atctcctgga tagtgtccac catcggcagg atgggctaca ccggagtggt cgccctgatg
     8101 ttcctggagt cgtccttctt ccccttcccc agcgaggtgg tggtgccccc cgccgggtac
     8161 ctggcctccc aggggcagat gaaccttttc ctcgtcattg ccgcaggcat tgcaggaagc
     8221 atactgggtg cactcttcaa ctactggata tccctcacct tcggccgccc gttcttccgc
     8281 aggttcggca agtacgtctt cgtaggcgaa aaggccctcg acagggcaga tgaattcttt
     8341 gcgaagcacg gccacataag cacctttatc ggcagactcc ttcccgggat ccgtcagctg
     8401 atctcgctgc ctgcaggcct ggcaaggatg aacatacccc ttttcctgtt cttcactacc
     8461 ctcgggtcgg ggatctgggt ggtcatcctt gcctacgtgg gctactgggt cggcaacaat
     8521 caggacctgg tccaccagta catgcatacg gcgacctata tctccatcgc cgcgtgcgcg
     8581 ttactggcca ttggttacgt gatccattac aaaaggaaga acaaaaaacg taacgcgtga
     8641 ctagtgaatc tttaatagtg aagcagaaaa ccgaaaggcg tgaacggtga acgggaaggg
     8701 caagttgctt gtcattctga gcgtaggaag aatctgggtt agctgccggt tctcacaacg
     8761 aaaaggtttt ctttggggtc caccctaccc tacgctggcg gcgcgccagc gtagggcgcc
     8821 ttgtctgtgg aaaaaggttt gtttttgccc ttgcaatggc ttttccgagt cacgaatcac
     8881 ggggttttgg gtttcccagt tacgagtcac aaggtgcccc aagcgcagcg acagggcgcg
     8941 tccctcagct tgtctgaggg gtacgagtta cgccttttgc ccttaggctt ttcctgcctc
     9001 gtgcagcctg tctactgtcg ccttgtttca ggtttccgag tcacgattta cggctcctca
     9061 atttgttccc ctaaagcata caatttgacg attcgtgaca ttcagtgtat actccttagg
     9121 ctgaaaaaag cgcctgtgat ctcaacgaaa ctcatgaaat tctccaggta acaccgcaca
     9181 aagtatctcc gccggttatt cagggtttca ggcgtttccc cttaaccgcc catccagggg
     9241 cgggaagttt cctgttgccc gggggataag ggcatttcag tctgaatata tcgaggtggt
     9301 agggtgagta gtccaaacgg cgcaagacgc gttgtaaaca cgcttgaatc cctgggtgtt
     9361 ttcgtaattt ttggcatccc ggggatacat aatctggata tttacaccgc tctcacggag
     9421 agcgagatcc gccacgttac ggcccgtcac gaacagggag cgggttttat ggccgatgga
     9481 tatgccagat ccaccggaaa gacgggggtg gccctggtga tctccggccc cggtcttacg
     9541 aacattctta cccctatggc gcaggccctt cacgattcca tccccatgct cgtgatatcc
     9601 agccagatac caaccccctt cataggcagg cgctgcggct ttctacacga acttcccgac
     9661 agcgcctctc tcgcacaagg agtctccaag aagagcttct gtgccaggac accggaagag
     9721 gtccctgctg tgatccggtc ggcatgggat cttgcctctg acggtcgccc gggccctgtc
     9781 cacgtggaga tccccatgga tgttttgggg cagaccagcg gcggtaaggt ctcttttcaa
     9841 gactcacctc ccccctaccc ggattttctt atccccgaag gtgctatgac ccggactgct
     9901 gaccttctgg acaactcctc caacccagtg atcttcgttg gcggcggctc ctgtggagca
     9961 agtgaagctc tctccaaact cgcggaaata tccggtgcca tggtcatcga gacctgtgcc
    10021 ggcaagggca ctgtcgatga aagaaacccc ctctgcctgg gagcaaggtc tcatttcccc
    10081 tgcgtcaggg aggctctttc agaagcagac ctcattctcg ccttcgggac ggaactctcg
    10141 ccgacggacc tctgggaaaa acccctcccc aaaaacgtta ccctggtcca ggtcgatctc
    10201 gaccccgcca atttcagccg gaacggtatg gccgatatcg ggataaccgc cgacgcgaac
    10261 gagatagctt cggctttgct gcagatggtc tctcccaacc cttccagatc agcggggaca
    10321 aaagagcagg tcagcagact tatagaaaag agcagcgaag aactggccac cgtgacgggc
    10381 atgggagacg aactagactc catgaatgca cttgtctcag ctatccgcaa agcgatcccg
    10441 gaggaaggga tcctcttcgc cgacatgacc ggcccagcct atgtagccat aagcgagtac
    10501 ccggcatatt ctcccaggac tttcctgcac cccgtaggat tcgggaccct cgggctggcg
    10561 cttcctggcg ctatcggggc cgccatcgca agaccttcaa gacccgtctg cgtcatggcc
    10621 ggtgacgggg gttttcagtt caccctccct gaactcgctg ttgcggtcca ggaacgtctc
    10681 ccgatccctg tggttatatg gaacgaccat ggcttcgggg agataaggag aaacgagaat
    10741 gcccggcatc ccggtatgtt catagccgtc gacaacaccc caccggacct tgccctcctt
    10801 gcttcggcat acggcgcaaa gtactccagg ccggagactc ccggggacat ctccgcttct
    10861 atcaggcagg ccttctctgc cggagtcccc acgataatcg agtgcaccac gggaaatgga
    10921 ggggtccact gatgaccgtc aagggactgc gccctgaagt cctttccatg gagacctatg
    10981 tcccgggaaa gaccatagag gaggtccgaa gggaactcgg gcttgaaagg gtggtcaagc
    11041 tcggttccaa cgagaatccc tacggaccct tcccaaaagc tctggaggca atgatgaagg
    11101 agctggccga agccaatcgc taccccgaca tcgccttcga ggagataaag ggactccttg
    11161 caaagctcca cggggtcacc cagaaaaaca tagcaatatc ccacggtgcc gaggggatgc
    11221 tacaggccat gggtaagtgt ttcatctccc ccggggacga ggtcctgata ccgtcggcga
    11281 cctacggcct ctacagggag atctcaaggc ttatgggggc aaaggtcgtg aacatcccca
    11341 tgaacgggta ctcaacggac atgaagggca tcgccaatgc cgtgactgac cggaccaagc
    11401 ttgtctggat ctgcaacccc aacaacccca caggcacggt catcgatccc gagagcctgg
    11461 aaaagttttt catggccctt ccagaggacg cctgggtcgt tctcgacgag gcctacgccg
    11521 agttcgcgtc gcccgaagag ctcccggaca gggccaggct catatctgaa gggaagaacc
    11581 tggtctcggt aaggaccttc tccaaggcct acggattggc aggggcccgg ctgggctacg
    11641 gcatggcccg ggagaaagtg gtgacggcaa tagacaccgt aagcgaaccc ttcaacgcga
    11701 acaggctggg gatcgccgcc ggggttgccg tactgacccg ggatcgtgaa gcctgcgaaa
    11761 gggcaagggc tcttatcctg accgaaaggg aaagggtatc gcaacacctt caggccatgg
    11821 ggatgagcgt ggtcccatct aggacgaact tcatcttctt cgacaccccc ttccacgccg
    11881 aaaacctggc tgaagagctt ctgtatcggg gagtgatcgt tcggccctgc ggtgggtggg
    11941 gttaccaggg tgccatccgg gtcaccgtcg ggacagaaga ggagaacgac atcttcctgg
    12001 cggccctgaa ggagactgtc gaaacgatga gagaaaaaga tagcggaacc ggttcccaca
    12061 gggaggtaga agatggtcct gttcgatagc gttacgaaag tttatgaaga cggcacccgg
    12121 gcagttgacg ggctcgacct ggagatcggg cgcggagaga tcgccgttct gataggcccc
    12181 agcggatgtg ggaagaccac aaccctgaag atggtgaacc gcctggaaga actttcctct
    12241 gggaggatcc tcgtcgacgg aaaggacatc aactccctgg atccggtcca gcttcgaagg
    12301 tccatcggct acgtggtcca ggagatagcc ctcatgcccc acatgtcggt ggccgagaac
    12361 atagccatcg tccccaggct gctggggtgg gacaagaaac gcataaagaa gcgggttgac
    12421 gaactgctgg agcttgcgag gctcgaacct tcaaagtacc gctaccgcct ccccgaccag
    12481 ctcagcggag gacagaagca gcgaataggt gtcctgagag ccctggccgc cgaccccgag
    12541 gtaatactca tggacgaacc tttcggagcc cttgacccaa taagccgtga ggggctccag
    12601 aacgaacttc ttgaactcca gaatgccgtg aagaagacca tcgtcttcgt gacccacgac
    12661 atggaggagg ctctgagaat ggccgaccag gtggtcatca tgcgtaaggg gcgcatagag
    12721 cagatgggta cgcccgagga gatccaggac cgccccgcca cggagttcat caggaacttt
    12781 atcggcgagg acaggctatc caacatctct cccgaatccc cggtggagat cctcgtccag
    12841 gacccggtga tcaaagcccg ttctgattcc atggcctctg aactgctgga ccttatggaa
    12901 gaagaggggt gcgagaccgc ccaggtggtg gacaagaaag gcaagtggag agggatggcg
    12961 ctgctctggc tgatcaagcg tgcggccagg agcggcctcc cggcttcgga cggcatcacc
    13021 cgggacagga aactgcaggc aggcgattcc acactgcgcg acgcggctga gatgcttgcc
    13081 gaccaggatc tgccgatccc tgttatcagc gataccggag accttttagg ggtcgttacc
    13141 caggccggta tagcccggct gaccatcggc aggcttacga ggaaaaggcg ggaggtgcga
    13201 gaataatgtt tcagcagaga cctttctggg aactcttctc ggcctatgtg atccgtttct
    13261 ggcctaggat actccagctc acatggcagc atatcgtcat ctcctccatt gctctcgcta
    13321 taaccctggt ggtatgcatc ccccttggga tctacctgac gaagaacgaa aaactggccc
    13381 cctatgccat aggggtggcg aacatcttcc agaccatccc cagcctggcc ctcctgggct
    13441 ttctgatctt catcttcggc atcggcaacg acaacgccat ctgtgccctc ttcctttacg
    13501 ccatgctgcc ggtgatacag aacacctaca cggggctcaa gaatgtaccc agacacctga
    13561 tcctcgcggc caggggcatg ggtatgaccg agtaccagat actgacccgg gtccagctgc
    13621 ctattggtct tccagtgata cttgccgggg taagggtagc cacggtatgg atcatcggga
    13681 cagccacgct ggcctcggcc attggcggag gtgggcttgg aaggctcatc ttctccggcc
    13741 tggcctctat aaggaatgag gtagtactgg ctggcgcact tccggccact cttcttgccc
    13801 tcggggccga ccagcttctc aagttgttcc aggcctactc ggacccgaag aagagggcta
    13861 tgaggatctc caaaaggtcg ggaccatccg gggacaggcc gtagaaagat cctgtcaaca
    13921 atatcagaaa ggggacatgc gttatgaaag gaaagaccat gcttttgatg gtcgtggcgg
    13981 ccttcgtaat tgtcgtaggc cttgtggtga tgaacaacgg gaaacaggaa gtcccgcccc
    14041 cgcccgaaga ggcgatagtc gccgagacgg accccgcaga ctatgagggc accgtcagga
    14101 taggcgggca gaccgttaac gagtccattg tactggcatg gatgggcaag ctcctcatcg
    14161 agaaccacac ggggctgaag gccgatataa acacggagtt cgctgcatct tcggtccttc
    14221 accaggccat gtcaggcggg gaactggaca tctacccgac ctggaccggc acccagctga
    14281 caggggtcct gcgatacgaa ggacccaacc tcccttctga tgagaccttc cagatggtca
    14341 aggaaggatt cgaggagaac ttcaacatga cctggacctc ccccatagga ttcaacaata
    14401 cctacgtcat ggccgttcga agggagaccg ccgagaagct ggagctcgag aaggcctcgg
    14461 acctcgctga ccacgcctcg ggaatgagac tggccggcga tgagaacttt gacacccggc
    14521 ccgacgccta cccgggctgg tccgaggcct acgggataga gttcggcgaa gtcctgccga
    14581 tgcagtactc catcatgtac agggccatcg ccaacgaaga ggtcgatgtg atcgcggcat
    14641 actccacaga ctcccgcatc aagaaactgg atctggccct tctggaggac gacatgtcct
    14701 tcttcccgga ttacagcgcc gctttcgtgg tcagaatgga gatcctcgaa gagtacccgg
    14761 agcttcttga tgtactggag aagctcagcg ggaccatcga cgaggtcaca atgtcggagc
    14821 ttaacggcag gttcgacgac ggcgaggaac ccgaggatat cgccaaagac ttcctctccg
    14881 gggccgggct tatctagaac aaaaaaccca gacacggggc cgtctccttg gggggcggcc
    14941 cttttgcttt tgcctgattc cccttaacat ggtacattgt gccccgaaga gagtagggac
    15001 tattctaaaa aatgtgaggc gatacgccat gtccatgaga gatattttcc agggttctcc
    15061 ctttggaggc gggggagggg aaccaaggag acagatacgc cccctcaggt tcaggaagag
    15121 cgtgtggatc cttttcggga tcgtcctgtt gctggcggtg gtcattcccg ccacggcaac
    15181 cttttacacc gacatgctct ggttcaggga gagggggctc tcccaggtat tctggaccag
    15241 gcttataccc cagtggatcc tctttgccgt agccgcagtc gcagccttcg ccatcttctc
    15301 cctgaactgg ctcaaggcgc gcaggtctgc aataaaggat ctggcctcct ccctccccga
    15361 agaggcggga gatatgcccc ttagggcttc ggcagtggtg gtcgcgatca tcgccggggc
    15421 gctggccgtg atgaacgggt tggggatacg ctcggagtgg atgacggtcc ttcagttctt
    15481 taacaggact ccctttggga aaaacgaccc gctttttggg aaggatattg ccttctatgt
    15541 tttcgagatc ccattcctgt ctatgctcca gggctggctt ctcaacacgc tgatcatggc
    15601 cctgatggga gttgccctta tagtcttcct ggcagccttc ccgaaaatgc gcgaggagaa
    15661 ccggatatac ctcccatccc atgccaggag ccacctctcc atcctgggcg ccgtaaccgt
    15721 ccttgtctgg ggggccggga tgtggctaga gcgtttcaac cttcttctct ccccggaagg
    15781 agttgtcttc ggggcgggtt acaccgatgt tcatgtccgg ctgttcgcga tcaacgtgat
    15841 gatagccctc tcggtcatcg ttgccgcgct cctggtggcg aacctttaca aaaggacctg
    15901 gaggctggca attgcggggg gtatactcct ggtcggcgca agctttcttc tcaggggagt
    15961 ggtcccgggc ataatccaga agtacgtggt ggaacccaat gaattctcca aggaacgccc
    16021 ctatatcgag ttcaacataa acagcaccct ggaggcctac gggctggact cccttgagat
    16081 catcgacttt agtccggaga acgccataac cagggagaac atagacaacg aaccagacac
    16141 cataaggaac atcaggctct gggactaccg accccttctg agggccttca aacagctgca
    16201 ggagataagg acctactacg acttccccga cgttgatatc gcacgataca ctttcgacgg
    16261 ctcctacaga caggtgatgt tggctgccag ggaactcgac cacggacagc tccagaaccc
    16321 cacctgggtc aaccggcacc ttgagttcac ccatggctac gggatagtca tgaatttcgt
    16381 gaacgaggtg gacagcgcag ggaaaccggt gctgatagtc aaggacctcc ccccgcagat
    16441 gtcggtcccc ctcaggatcg agaagccgca gatctactat ggggaaaaaa ctaaccccta
    16501 ttcactggtc cgcaccagca tcctggaatt cgactacccc atgggagatg ccaacgtaag
    16561 gacgacctac gaggggaccg gaggcgttcc tatcgggaac cttttccgaa ggatcatgtt
    16621 cgccctccgg taccgtgatt cggagatcct cttcaccaac gtcataacct ccgaaagcag
    16681 ggtcctgatg taccgaagca tccgggaacg cctggacaag gtagcgcctt tccttctcta
    16741 tgacagtgac ccctacctgg ccatcatcga cgggaggctg gtgtggatcc aggacgccta
    16801 caccgccacc gaccgctacc catactccaa ccccgtgagc gtaacaggga ccctgcagag
    16861 gattaattac atccggaaca gcatcaaggc tgttgtagac gcttatgacg ggagcatcct
    16921 cctttatatt tccgacggtg aagatcctat gatcaggaca tgggcgggta ttttccccgc
    16981 gcttttcaca cctatcgagg atgcccaccc cgaactttgc acccagatcc gctatcccat
    17041 ggctcaattc cttatacaga gcgagatctt ccgcacatat cacatgagcg accccaacac
    17101 cttctacaac aaggaggacg tctggcggct ggacaatcag acgactacag gaagcctcaa
    17161 cgcctactac ctgataatga ggc
//
LOCUS       DAOPKQ010000006        16875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_3606_length_16875_cov_4.730083, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000006 DAOPKQ010000000
VERSION     DAOPKQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 16875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16875
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3606_length_16875_cov_4.730083"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK146_00465"
     CDS             complement(<1..205)
                     /locus_tag="PK146_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HPE90739.1"
                     /translation="MTGGTDMGDKGRLVIVSNRLPVRIEGHPGRWKVTHSSGGLVSAL
                     TPVLRDRGGIWIGWTGISEMLDMK"
     gene            complement(202..1464)
                     /locus_tag="PK146_00470"
     CDS             complement(202..1464)
                     /locus_tag="PK146_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE90740.1"
                     /translation="MGLPYIARLEHYEPYIGKEAVERILLKAEMMSDRRIVHINSTYY
                     GGGVAELLGSMTLLANLAGIQMGWRVIQGSPDFFSVTKKMHNALQGGEINLSWKKFRI
                     YEHVVFENVLRNHLDHDIVVIHDPQPLPMVRHYHKKGPWIWVCHVDLSNPNRKLWPYI
                     RNYVNRYDAAVFSIPEYRQDTRTPLVFIKPAIDPFSIKNRDFSEEEIKERLDRYDIPT
                     DKPLVVQVSRFDKWKDPAGVIEAFKIARKEVDCNLVLLGNIATDDPEGVEIFNELKGK
                     QEEGILMLTVEDTALVNALQRKAAVVLQKSIREGFGLTVTEAMWKGKPVIGGNVGGIR
                     HQINDGENGFLVDTVEEAAERIVMLLKDPDLCRSIGEKARRTVQEKFLMSRYLEDYLD
                     LFKSFEASYTLREPLWNKRSRPGKRNRR"
     gene            1722..2177
                     /locus_tag="PK146_00475"
     CDS             1722..2177
                     /locus_tag="PK146_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPE90741.1"
                     /translation="MVLVGFGCVILSALFFGSLGPAERVAFGYGVTPAGVSLVRAVVG
                     AVVIGSYALFREPSALVPGVRDSWKFALAGLFGVVFVYYLSNIAFVTIPVGLTVILFY
                     TNPIWTALGAAAMGKEPFTRARAAALVAGLAGVWIAVGGTGVQKSGNWT"
     gene            2174..2671
                     /locus_tag="PK146_00480"
     CDS             2174..2671
                     /locus_tag="PK146_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE90742.1"
                     /translation="MTGVALAVVSGVGFSFYILNSRYGTGRERPFKTFVQMFLWGALM
                     MLAIVLYSREMPDFRDLPLPALLSLVHLALFPTLVSYGLISFALKYIPGTVASITSMS
                     EIIFAGVMAWVFLDEVPSPDQIRGGLLIVTAVLLLLMEGRISRSGSEGDKKRGPLQRG
                     PRDAA"
     gene            complement(2678..4468)
                     /locus_tag="PK146_00485"
     CDS             complement(2678..4468)
                     /locus_tag="PK146_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90743.1"
                     /translation="MKPTTARMLFTAAFLALTVLVSGTAWGAMGEVTSFAFRTAQDDV
                     LGLERSLELDGKPDAHFVLSLKGIGAISDVSLRTVDGKREWSPAASGDRWTMVVRDAS
                     GKTVSSESGMPITPFLGFATLNLYVSDDGTAFSETREYEVSVKFIDGSTTSAKIEVKG
                     MPDVFGSPEPLQPESGVMTATVYGIGNRDVAGKRETLGKDGTNDAHFRVRFTTISVVD
                     QITIRNVDGTNAMWDTVSGNGAWAIAVFQEGKLRNKADGSVRFAVDGDTTLDLWVADN
                     GAVSGGKTRFEVIIKFNDGTTFREIASPGTPEVEASVEDILSAVLYAPATTDIANRGE
                     TPGVNGTPDWRITLSVAGEGTIISFIVRGIEGSVAEWDTLPGNKKPIAVVTDSAGKIL
                     NSSNGSVSIPLSGRKDLSIWLDDAGALSDGANKFRVIAVLSDGRTFERLIERAATQTS
                     VPPGSENVRESDSVRAFYMGKGPRNLIGKGEPADITKGLDANPDAHIRLRLVSLNGTI
                     DSLTVESLNGNGGDWDTIPGNGRWHIAVTETAIGPVLNKNDGSFSKKVSGNSEFHLWL
                     ADNGKLSANPSSYRVIVRFSDGRALSQNLF"
     gene            complement(4555..5067)
                     /locus_tag="PK146_00490"
     CDS             complement(4555..5067)
                     /locus_tag="PK146_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HPE90744.1"
                     /translation="MSERIAVLVENDFHDIEFWYPYYRLQESGHEPIIVAPTAPKVYR
                     GKYGTTVEAAYDAGSIAGKSLAGVIIPGGWAPDRLRMSAEIVGLVKRTYESGGFVAGI
                     CHAGSLLVSCGVLDGKTVTSYPSLRDDMVLAGAEWMDLEVVVSDRIITSRRPSDLPAF
                     MAEVMRILKG"
     gene            5268..5606
                     /locus_tag="PK146_00495"
     CDS             5268..5606
                     /locus_tag="PK146_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE90745.1"
                     /translation="MKITSFKESAMITTPHGVKVCKLYDTSDAQAIHITLEPGESLRK
                     HITPVDVFFYVLEGTGVVEIGEEKSEVSRDALIESPRDIPHCWYNESDATFRVLVVKA
                     PRPVASTKLL"
     gene            5652..6833
                     /locus_tag="PK146_00500"
     CDS             5652..6833
                     /locus_tag="PK146_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPE90746.1"
                     /translation="MVLEIKKNVFWVGVKDWALSHFHGHELSTHRGSTYNAFLINDKK
                     IAVVDTVWDPFTDKFIENVRSLVDPSKIDYVVANHAETDHSGALGALMELAPKAEVVV
                     SPRGMESVPGHFHRNWKFRQVKTGDSIPLGETEMIFVEAPMLHWPDSMFTYLSGRDLL
                     MPNDAFGQHYASAFPYNDQVDQKELFEECLKYYANILTPFSPMVLKKIDEVLALGLKV
                     DMIAPSHGVIWRKDPLQIVKKYQEWARQEPERSAVILYDSMWNATRDMADAISEGLAE
                     KGVSSKVFHMALTDRNDVITQIFRSRAVIVGSSCINGGVLPTITPILEDLRGLKFRNK
                     TGAAFGSYGWSGENVARIETHLEEAKIPLAVGGLNLKWRPDAEGLEKCREMGREIAGK
                     M"
     gene            7018..9465
                     /gene="gyrA"
                     /locus_tag="PK146_00505"
     CDS             7018..9465
                     /gene="gyrA"
                     /locus_tag="PK146_00505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPE90747.1"
                     /translation="MEKKPGELSFGKVIPLPIEEEIKHSYLDYAMSVIVGRALPDARD
                     GLKPVQRRVLYSMLELGLRHNQPYKKSARVVGETMGKYHPHGDSAIYDTMARMAQDFS
                     MRQTLVDGQGNFGSIDGDPPAAMRYTEARLSSAGEQMLADIDEETVDWGPNFDDSLKE
                     PLTLPTRVPNLLVNGSTGIAVGMATNMPPHNLGEVIDALCLLLDNPDAGVEEILSVLP
                     GPDFPTGGVVLGRQGIHDAFRTGRGKLTIRGKAHVEEGRKGKNSIVITEIPYMVNKTN
                     LLEAIAKGVQAKTIDGITDLRDESDRRGIRVVMELARDANDEVIMRQLFRRTQLQTTF
                     GVINLALVDGRPLELSMMAMLGLFLDYRREVVRRRTVFRLRKAEERAHIVEGLIRAID
                     IIDDVIALIRASKDPQEARAGLVGTLDFSEAQAQAILDMRLQRLTNLEKNKLEEEFAQ
                     LLADIEKYRSILGNPATLDMVVREELAEIRSGFADDRRTTIVDAVDEVSLEDLIPEEE
                     IVVVLSRDGYLRRMPLEQYRLQGRGGKGVKGATPKVGDEAAIISVSSTHRDIYLFTTK
                     GRVFAVKGHAIPEPRTGKGRLASQLISLENGESVVGMKDSHLHGAKFVFFITARGVAK
                     RLPAEELENLTRAGRRVLTVDEGDEIARVRFSSGNDDIFIVTAKGQALRVSESEFRPM
                     GRQARGVRGIRLMGGDLVVGCDIVKPGHQVLLVSERGVGKRTCYDEFMPHHRASGGCR
                     AMKISPKTGKIVGSWGLMPDDQIIVMTNRGRVIRLDVDEISILGRTATGYRVIKVAEG
                     DEVADISVIRTSEEEGE"
     gene            9469..10101
                     /locus_tag="PK146_00510"
     CDS             9469..10101
                     /locus_tag="PK146_00510"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPE90748.1"
                     /translation="MRLAPEGRGPVLISGGFGVISFLLFPWWVAVLPVVLAAFLAWFF
                     RDPERFPPEDVDGWVSPADGRVVEIYASEHPFTGRCMVVGVFMSPLDVHVNRMPYDGE
                     VAFLEYLPGKKLMAFNPKASQENERFFMGFETGSGKGMTVQIAGFLARRIVCSRKKGE
                     VISRGERFGMIKLGSKVDLYLPEGMIPAVKKGEKVRAGETVMGVRKKDEA"
     gene            10091..10831
                     /gene="pssA"
                     /locus_tag="PK146_00515"
     CDS             10091..10831
                     /gene="pssA"
                     /locus_tag="PK146_00515"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPE90749.1"
                     /translation="MRRKNRGKPVPMKTIIPNMITSGNMLCGMLSLVLAFHGHLVPAS
                     WLIFIAVIFDFMDGKMARKLGGSSSFGMELDSLADVVSFGVAPAMVMYSVYLKGFFGV
                     TGAIVAAFFALCGALRLARFNVHHVDGSFQGLPIPAAGHFVVSFVVAGIYLPPAVMAV
                     IVAVTGFLMISNVSFGNLKALKPGNMDRRKATFLWAFAFMLIAVLRNKAPLAGILIYI
                     VSGFAGVDWGKWLSLEAVDEENLAERDA"
     gene            10871..11116
                     /locus_tag="PK146_00520"
     CDS             10871..11116
                     /locus_tag="PK146_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPE90750.1"
                     /translation="MNHLIGYLVVGAIAGWLAGKVWQGRGFGFFGNIGVGVIGAFIGG
                     VIFSSIGIYPMGFVGSLVMAFVGAIVLLFLVGVLKRK"
     gene            complement(11166..11669)
                     /gene="thiT"
                     /locus_tag="PK146_00525"
     CDS             complement(11166..11669)
                     /gene="thiT"
                     /locus_tag="PK146_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HPE90751.1"
                     /translation="MNNRKVRILVEGAMAAALALALSYVRIWRMPQGGSITLENVPLF
                     LFTLRWGLKAGLGAGSVAGLLQLILGGYVVHPVQALLDYPMAFSVLGLASLCRRPLWA
                     GLALGALARFLCHVLSGVVFFAAYAPESTNVWIYSAVYNGSFMLPSLVLSVVAIYMIW
                     PRLEKIS"
     regulatory      complement(11771..11879)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12032..13195
                     /locus_tag="PK146_00530"
     CDS             12032..13195
                     /locus_tag="PK146_00530"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPE90752.1"
                     /translation="MIFLITFFCPRCGSIFDGTVRPVGCSCGAPLKSSIDWTDLDIDQ
                     SAPGMARYASALPPLDPAVSMGEGWTPIVRSTTGGPFYKLEYLSPSGSFKDRGSVLVM
                     TEAKALGCGSVIQDSSGNAGASIAAYAARCGIKATVIVPRDITEAKKRQIELYGAVLE
                     VVEGDRKTAASLALSLSDSTYYASHVWNPLFLEGTKTVAFEIWEQLHFRAPDAVVVPA
                     GNGTMLLGVFKGFKELLQRVRIKRLPRIYAVQGSHCAPLHAQWNETGEKVFGGTLAEG
                     IAVTDPPRMKEMLDALRVSRGDVLVVEDETILLEQERLALEEGLLVEPSAAVAPAAER
                     MLRGAGMISSGETVVVPLTGSGLKKIPSRRPKKQASPDEGRPLFDNLGRKIRG"
     gene            complement(13185..14162)
                     /locus_tag="PK146_00535"
     CDS             complement(13185..14162)
                     /locus_tag="PK146_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HPE90753.1"
                     /translation="MDMLYLNGEQIESMRIPIREVIEVIEEGFQLKGEGLVQLPSSQG
                     VYPREDCFLDAMPAYFAGSADTVGIKWVSGYPSNLEKGLPNIMGLIVINDAETGAPLA
                     VMDAKWITAYRSGAATAVTAKYLAPEKASTLSIIGLGVQGRTNLLGLRETMKGLRRVT
                     VYDKSEANARKYLEDMQSKVHGAEIELCPDARSAVKDADVIVTCTPVHRKPNRFIKAD
                     WLKKDVLAVSANNDSAFEEEVMTGASFFVTDDRGQYLQTQRAGTYFRGYPEEVPADMG
                     EICAGIVEVPRTPGRKAAVLMGIGSHDVATARVIYEKAQRIGLGIRLNL"
     gene            14325..15248
                     /locus_tag="PK146_00540"
     CDS             14325..15248
                     /locus_tag="PK146_00540"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPE90754.1"
                     /translation="MRKAREIDVSLVSEAVKGLFIKANVSIGQDVYNALVQARDRIEG
                     SPIGRSLDPVSGLDQGIDRIEGSPIGRSVLDQIIRNHDLARTEQMPICQDTGMAVVFA
                     DIGQDVLLTGGDLSAAVNLGVEEAYREGFFRKSVVEDPLFDRRNTGTNTPAVIHTRII
                     PGDRVKISVTPKGFGSENKSALRVFTPAQGPGEVRDFIRDTVAAAGPDPCPPVLIGVG
                     IGGTVESAAIMAKRATLYPVGRRNPERRYAALEMETLEAVNALGIGPAGLGGTVTCLD
                     IHMDFAPGHIAGTPVVVNICCHAARHAEVSI"
     gene            15259..15822
                     /locus_tag="PK146_00545"
     CDS             15259..15822
                     /locus_tag="PK146_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPE90755.1"
                     /translation="MMRYASRIVAPLNDEMVLSLRSGDKVLISGVIFTGRDAAHRRMA
                     ENIAENRPLPFDVRGQIIYYVGPSPTPPGRVCGSAGPTTSGRMDPYTPLLLDLGLKGM
                     IGKGKRSPDVIESMKKNRALYFGAVGGAAVLISKCINRMETVAWPELGPEAVFRMRVE
                     DLPVVVLVDSFGNDLYSDGPAGFRRSE"
     gene            15788..16564
                     /gene="pxpB"
                     /locus_tag="PK146_00550"
     CDS             15788..16564
                     /gene="pxpB"
                     /locus_tag="PK146_00550"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HPE90756.1"
                     /translation="MTVLPGSVAANSHCFNNPRKGGSDLEGGTGYPRFSWCGDACLVA
                     EFGDKIDPLINSMVRDLDLRIRGLKLPWLVETVPTYRSLAVYVDPWDSPREEVELAVT
                     SLAREVKAKTMPSVAVVEIPVCYGGDFGPDLERVSMYTALGPDDIIRLHSSKLYRVYM
                     MGFTPGFPYLGGMDPRLETPRLEDPRTLIPAGSVGIAGKQTGIYPIASPGGWNIIGRT
                     PLVLFDPAGASPFLLEAGMEIRFVPVTGERFEEILSGNGA"
     gene            16569..>16875
                     /locus_tag="PK146_00555"
     CDS             16569..>16875
                     /locus_tag="PK146_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KipI antagonist"
                     /protein_id="HPE90757.1"
                     /translation="MSVEIISPGVLSTVQDLGRYGFQAFGMPVAGALDRYSLMVGNLV
                     VGNDLRSAGLEITISGPELLFRSERLVCITGGDLSPKINDRDVPVWQGLMLHEGDVLS
                     "
BASE COUNT         3481 a         4674 c         5021 g         3699 t
ORIGIN      
        1 tcttcatgtc aagcatctca gagattcctg tccagccgat ccatatgcct ccacggtcac
       61 gaaggaccgg ggtcaaagcc gataccagac ctcctgatga gtgggttacc ttccaccggc
      121 caggatggcc ctctatcctg acaggaagcc tgtttgagac aatgaccagc cttcccttgt
      181 ctcccatgtc cgttccaccc gtcatcgacg gttccttttg cctggtctgg atcttttgtt
      241 ccagagcggt tccctgaggg tgtagcttgc ctcgaaggat ttgaagagat cgaggtagtc
      301 ctcgaggtaa cgggacatga ggaacttctc ctggaccgtc cttcgggcct tctccccaat
      361 actgcggcag aggtccgggt ccttgagcag catcactatc ctctcggcgg cctcctcaac
      421 ggtatccacc aggaaaccgt tctccccgtc gtttatctgg tgccttatcc ctccgacgtt
      481 ccccccgatc accggtttgc ccttccacat ggcttccgtc acggtcagac cgaaaccctc
      541 ccggatcgat ttctgaagga ccacggcggc cttgcgctgc agtgcgttca ccagcgcggt
      601 gtcctccacg gtgagcatca aaatgccctc ttcctgcttt cccttcagtt cgttgaagat
      661 ctccactccc tcggggtcgt cggtggcgat gttgcccaga aggaccaggt tgcagtccac
      721 ctccttgcgg gcgatcttga aggcctcgat cacccctgcg gggtccttcc acttgtcgaa
      781 acgcgatacc tggaccacca gtggcttgtc ggtggggatg tcgtacctgt cgagcctttc
      841 cttgatctcc tcttccgaga agtccctgtt cttgatgctg aatgggtcta tggcgggctt
      901 gatgaagacc agcggtgtcc gggtgtcctg cctgtattcg gggatgctga aaaccgctgc
      961 atcgtagcgg ttcacgtagt tcctgatata gggccagagt ttcctgttgg ggttggagag
     1021 gtccacgtga cagacccaga tccaggggcc cttcttgtgg tagtgacgga ccatcggaag
     1081 gggctgaggg tcgtggatca ccacaatatc atggtcgagg tggttccgaa gaacattctc
     1141 gaagaccacg tgctcatata tcctgaactt cttccagctg aggttgatct cccctccctg
     1201 aagggcgttg tgcatcttct tcgtgacgct gaagaagtca ggggagccct ggatgaccct
     1261 ccatcccatc tgaattccgg ccaggttggc cagcagggtc atggagccca gcagttcggc
     1321 gacccctccc ccgtagtatg tcgagttgat gtggactatc cttcggtccg acatcatttc
     1381 agccttcaaa aggatcctct cgacagcctc tttgcctatg tagggttcat aatgctctag
     1441 cctggcgatg tatggaagtc ccatgcggta tctctccttt caaagaacgg tgcctgatgt
     1501 ccggcgaacg tgtccgggtt ttcaagagga tccagaggat cttcaatagg gtgatctgaa
     1561 aagggctggg gatcggcgcc ggtgtcacag gggaaccgct gtaacgtatt tgtgtcatat
     1621 tataagacaa ttccgtgaat ttacgaaatc acgctctcct gttgggttac cggcccgtat
     1681 atgcaagaat gtctggagtt ggtcgtaaag gagttgtcgt cttggtactc gtcggattcg
     1741 gctgtgtgat actctcggcc cttttcttcg gctccctcgg acctgctgaa agggtggcct
     1801 tcgggtatgg cgtgaccccg gccggggtct ccctggtcag ggccgttgtc ggtgctgtgg
     1861 ttataggttc ctatgccctc ttcagggagc cctcggccct tgtaccaggg gtgagggatt
     1921 catggaagtt cgctcttgca gggctttttg gtgttgtctt cgtatactac ctgtcgaaca
     1981 tagcctttgt gaccatacct gtgggactta ctgtcatcct tttctatacc aatccgatat
     2041 ggactgccct gggagccgcc gccatgggca aggagccctt caccagggcg cgtgcggcgg
     2101 ccctggtcgc cggtctcgcc ggagtgtgga tcgccgtcgg aggtacgggg gtgcaaaagt
     2161 cggggaactg gacatgaccg gtgtagcgct ggccgtcgtc tcaggggtgg ggttctcctt
     2221 ctacatcctc aacagccgct acggcacagg aagggaaagg cccttcaaga cctttgtaca
     2281 gatgttcctg tggggggctc ttatgatgct ggccattgtg ctttacagca gagagatgcc
     2341 ggacttccgg gaccttcccc ttccagccct tctttctctt gtccatctgg ccctcttccc
     2401 gacactggta tcctacgggc tcatctcctt tgccctcaag tacatccccg gaacggtggc
     2461 ctccataacc tccatgtcgg agataatctt cgccggggtg atggcctggg tcttcctgga
     2521 tgaagtaccc tcgccggacc agatcagggg cggactgctg atagtgacag cggtgctgtt
     2581 gcttctcatg gaagggagga tctcccggtc tggatctgaa ggtgataaaa aaaggggacc
     2641 cctccagagg ggtccccggg atgctgcctg atctctccta gaaaagattt tgtgagagtg
     2701 cccgtccgtc ggagaagcgc actatgaccc tgtaactcga cgggttggcg ctaagtttgc
     2761 cgttatcggc gagccagagg tggaactcag agttgcccga gaccttctta gagaaggagc
     2821 cgtcgttttt gttcaggacc ggccctatgg ctgtctccgt tacggcgatg tgccatcttc
     2881 cgttacccgg gatggtgtcc cagtctcccc cgttgccgtt tagggattcc accgtcagtg
     2941 aatctatggt cccgttcagt gataccagcc tcaaccttat gtgggcgtcg ggattcgcat
     3001 cgaggccctt ggtgatatcc gcaggttctc cctttcctat caggttcctc ggccctttgc
     3061 ccatatagaa ggcccttacg ctgtccgatt cccggacatt ctcgctcccc ggcgggacag
     3121 aagtctgggt ggcagcacgt tctatgagcc tctcaaaggt cctcccgtca ctgaggacgg
     3181 ctatcaccct gaacttgttc gccccgtccg aaagggcgcc cgcgtcgtca agccagatag
     3241 agagatcctt ccttccgctc agcgggatcg agaccgaacc gttggacgag tttaatatct
     3301 tgccggctga atcggtgacc acggcaatgg gtttcttgtt gcccggaaga gtgtcccact
     3361 ccgcaacgga gccctcgatc ccccgaacta tgaaactgat gatggtcccc tctccggcaa
     3421 cggataaagt gatcctccag tcaggggtgc cgttgactcc cggggtttcg ccacggttgg
     3481 cgatgtcggt ggtcgcaggg gcgtagagta ctgccgagag gatatcctcc acggaggcct
     3541 ccacctccgg ggtcccaggc gaggcgatct cgcggaaggt cgtaccgtcg ttgaacttga
     3601 tgatcacctc gaaacgggtc tttccgccgg aaacggcgcc gttgtctgca acccagaggt
     3661 ccagtgtcgt atcaccgtca acggcgaatc gcacgctgcc gtctgccttg ttcctgagct
     3721 tgccttcctg gaacacggca atcgcccacg ctccatttcc ggagacggtg tcccacatgg
     3781 cgttggttcc gtccacgttg cggatcgtta tctgatccac tactgaaatg gtggtgaagc
     3841 ggacccggaa gtgggcgtcg ttggtcccgt ccttgccaag ggtctccctc ttcccggcga
     3901 catcccggtt tccgatcccg taaaccgttg cggtcatcac accgctctca ggctgaagcg
     3961 gttcgggaga gccgaaaaca tcaggcatcc ccttgacctc aattttcgcc gaagtggtgc
     4021 tgccgtcgat gaacttgacc gatacctcgt attcccgtgt ctcggaaaaa gctgtgccgt
     4081 cgtcggagac gtaaaggttg agcgtggcaa accccaggaa gggggttata ggcatccccg
     4141 actcagaaga gaccgtcttc cccgaagcgt cccttaccac catggtccaa cggtcaccgg
     4201 aagcggcggg tgaccattct ctcttgccgt ccactgtcct caaagagaca tcggagatag
     4261 ctccgatccc ctttagagag agcacgaagt gggcatcggg tttcccgtcc agttcaaggg
     4321 atctctccag accgagaaca tcgtcctggg ctgtcctgaa ggcaaaggag gtcacctctc
     4381 ccatggctcc ccaggccgtt ccagacacaa ggaccgtcaa agcaagaaag gcggccgtga
     4441 aaagcatcct tgcagttgtt ggcttcattt cctgtccctc cccggacctt ctttttttct
     4501 tcccaacttg aaacgattat aacattaacg ggttcaaagc ctctggatgg agcgtcaacc
     4561 cttgaggatc ctcatcacct cagccatgaa ggcaggaaga tcagaaggcc tcctgctggt
     4621 tatgatccgg tctgatacga ccacctccag gtccatccac tccgctccag ccaggaccat
     4681 gtcgtccctg aggctgggat agctcgtcac ggtcttgccg tcgagtactc cgcaggatac
     4741 cagcagactg cccgcatggc atatccccgc aacgaatccg cccgactcgt aggttctttt
     4801 aacgaggccc actatctctg cggacatcct gaggcggtcc ggtgcccagc ctcccggaat
     4861 gatcacaccg gcaagggatt tcccggctat gctgccggcg tcataggctg cttcgacggt
     4921 tgtcccgtac ttgcccctgt agacttttgg agccgtgggg gcgacgatga tcggttcatg
     4981 ccccgactcc tgcagcctgt agtaggggta ccagaactct atgtcgtgga aatcgttctc
     5041 caccagtact gctatcctct ccgacattgg cagtctcccc tctcgtgaag gatcggcatt
     5101 cctgtctctt gtcaacaaag attatacagc cttggacaag ttaggtgacc gggggtcttt
     5161 taggttatca ttttacagta gtgtcgcaat gtccctgagg gatccgtgcc tcttttgaaa
     5221 gacgcaggga cttcaggaga aatgccagga gaggagagtg gatcttcttg aagatcacca
     5281 gtttcaagga atcagcgatg ataaccaccc cccatggggt gaaggtgtgc aagctctacg
     5341 acacttccga cgcgcaggcc atccatatca cccttgaacc cggagagtca ctcaggaaac
     5401 atattacccc cgtggacgtc ttcttttatg tcctggaagg cacaggggta gtggagatag
     5461 gggaggagaa gagtgaagta tcgagggacg ctctcatcga gagccccaga gatatcccac
     5521 actgctggta caacgagagc gatgcaactt tcagggtcct ggtggtcaag gcccccaggc
     5581 cggtagcatc gacgaaactg ctttagcctg aggtatgaaa agggaccatt tcgaaaaagg
     5641 aggggaagca attggttctc gagatcaaaa agaatgtttt ctgggtggga gtcaaagatt
     5701 gggcactcag ccatttccac ggtcatgaac tctcgactca caggggcagc acctacaatg
     5761 ccttcctcat aaatgataaa aaaatcgcgg tggtcgatac agtctgggat ccctttacgg
     5821 acaagtttat cgagaatgtg agatccctgg tggatccttc gaagatcgac tacgtggtgg
     5881 caaaccacgc ggagaccgac cactccggag ccctgggagc acttatggaa ctggcaccca
     5941 aggcagaggt ggttgtctct ccgcgcggta tggagagcgt accgggccac ttccacagga
     6001 actggaagtt caggcaggtg aagacaggcg actcgatccc acttggagag acggagatga
     6061 tcttcgtcga ggctccaatg cttcactggc ccgacagcat gttcacctac ctttcgggga
     6121 gggacctgct gatgcccaac gacgccttcg gtcagcacta cgcttctgcc ttcccctaca
     6181 acgaccaggt cgaccagaaa gagctcttcg aggagtgcct gaaatactat gccaacatcc
     6241 tgaccccctt cagccccatg gtgctgaaga agatcgacga agttctggcc cttgggctca
     6301 aggtcgacat gatagccccg agccacggcg tcatctggcg gaaggacccc ctccagatcg
     6361 tcaagaagta tcaggagtgg gccaggcagg aacctgaaag gagcgctgtc attctgtacg
     6421 actccatgtg gaatgccaca agggacatgg ccgatgccat aagcgaagga ctggctgaaa
     6481 agggggtctc ctccaaggtc ttccacatgg ccctcaccga ccgtaacgac gtgataaccc
     6541 agatcttccg ctccagggct gtcatcgtcg ggtcctcctg catcaacggc ggagtgctcc
     6601 ctacaattac tcccatcctg gaggacctga gggggctcaa gttcaggaac aagacaggag
     6661 ctgctttcgg atcctacggt tggagcgggg agaacgtggc aaggatagag acccacctcg
     6721 aggaggcaaa gatacccctt gcggtcgggg gacttaacct taaatggcgc cccgatgccg
     6781 agggtcttga gaagtgcagg gagatgggaa gagagatcgc aggcaaaatg tagtatcctt
     6841 tctgccgggg ttccggggtg gcgccccggc agtttcgttg aagagtgtcc tttggtgtaa
     6901 aatatacctg cgggggcctt cggagctttc cgggggcccg aggcgcattc gccctgcggc
     6961 ggcgttcttg cccgccgctt tttgttgtta ccggcgctga aggagttggt cgaacagatg
     7021 gaaaagaaac ccggtgagct ctctttcggg aaggtgatcc ccctcccgat agaagaggag
     7081 atcaagcaca gttatctgga ttatgccatg agcgttattg tcgggagagc gcttcccgat
     7141 gcaagggatg gtctcaaacc ggttcagcga agggtccttt actcaatgct ggaacttggg
     7201 ctaaggcaca accagcccta caagaaatcc gcaagggtcg tcggggagac catgggcaag
     7261 taccaccccc acggcgactc ggcgatctac gacaccatgg ccaggatggc ccaggatttc
     7321 tctatgaggc agaccctggt tgacggccag ggtaacttcg gctccatcga cggcgatcca
     7381 cctgcagcga tgcgatatac tgaagcgaga ctatcgtctg ccggcgaaca gatgctggcc
     7441 gacatcgacg aggagaccgt cgactgggga cccaattttg acgactcgct gaaggagccc
     7501 cttacgcttc ccaccagggt tcccaacctc ctggtgaacg gcagcaccgg cattgccgtg
     7561 gggatggcaa ccaacatgcc gcctcacaac cttggagagg tcatcgacgc actatgcctg
     7621 cttctcgaca accccgacgc gggggtcgag gagatccttt cagtcctgcc gggccctgat
     7681 ttcccgacgg gcggtgttgt cctcggaagg cagggtatcc acgatgcatt ccgcactggc
     7741 aggggaaaac tgaccataag gggcaaggct cacgtcgaag agggccgaaa gggcaagaac
     7801 tcgatagtga tcaccgagat cccctacatg gtcaacaaga ccaacctcct tgaggccatc
     7861 gccaagggtg tccaggctaa gaccatcgac ggcataactg accttcgtga cgagtccgac
     7921 cggcggggta taagggttgt aatggaactt gccagggacg ctaacgacga agtcatcatg
     7981 aggcagcttt tcaggaggac ccagctccag accaccttcg gtgtcataaa ccttgccctg
     8041 gtcgacggcc ggccactgga gctctccatg atggccatgc tgggtctatt cctggactac
     8101 cgcagggagg tcgtccgtcg aaggaccgtc ttccgtctcc gcaaggcaga agagagggcc
     8161 catatagtgg aggggctcat cagggccata gacattatcg acgacgttat cgccctgatc
     8221 agagcctcga aagaccccca ggaagccagg gctggacttg tggggacact tgacttcagc
     8281 gaagcccagg cgcaggccat tctggacatg aggctgcagc gtctcacaaa ccttgaaaag
     8341 aacaagctgg aagaggagtt cgcccagctt ctggccgaca tcgaaaagta caggagcatc
     8401 ctgggtaacc cggctacact ggacatggtc gtaagggagg agctggcaga gatccgttcc
     8461 gggttcgctg acgacagaag gacgaccatt gtagatgcag ttgacgaagt ttccctggag
     8521 gacctgatcc cggaagagga gatcgtggtc gtgctctcca gggacggcta tctaagaagg
     8581 atgccccttg agcagtaccg tctccagggc cggggcggca agggggtcaa gggggctact
     8641 ccgaaggtgg gagatgaggc tgccatcatt tccgtatcct caacgcacag ggatatatac
     8701 ctctttacaa caaagggacg ggtctttgcg gtcaaggggc acgccatccc cgaacccaga
     8761 acggggaagg ggaggcttgc cagtcagctg atctcccttg agaatggcga gtccgtggtg
     8821 gggatgaagg acagccacct tcacggagca aagttcgtgt tcttcatcac cgccagggga
     8881 gtggccaaga ggctcccggc cgaggagctg gagaacctga cccgggcggg cagaagggtg
     8941 ctcaccgtgg atgaagggga cgagatagcg agggtccgtt tctccagcgg caatgacgac
     9001 atattcatcg ttaccgccaa ggggcaggct ctcagggtct ccgagagcga attccgcccc
     9061 atgggccgtc aagccagggg ggtacgggga atacgcctta tggggggtga tcttgtcgtt
     9121 ggatgtgata tcgtcaagcc cggtcaccag gtacttctgg tgagtgaacg tggtgtgggg
     9181 aagaggacct gttacgacga attcatgcct caccacaggg caagcggtgg atgcagggcc
     9241 atgaagatat cacccaagac aggcaagatc gtgggatcct gggggctgat gcccgatgac
     9301 cagatcattg tcatgaccaa caggggccgg gtgataaggc ttgacgtcga tgagatctcc
     9361 attctgggcc ggaccgcaac gggatacagg gtcatcaagg tggcagaggg ggatgaagtt
     9421 gcggatatca gcgtcatcag gacaagcgaa gaagaagggg aataggggtt gagacttgca
     9481 ccggagggac gcggtccggt tctcatatcc ggaggttttg gggtcatttc gtttcttctc
     9541 ttcccctggt gggtggctgt cctgcctgta gttctggcgg ctttccttgc ctggttcttc
     9601 agggacccgg aaaggtttcc gcctgaggac gtggacggat gggtcagccc agccgacggg
     9661 agggtcgttg agatctacgc ttctgaacac ccctttacgg gcagatgcat ggtggtaggc
     9721 gtcttcatgt cacccctgga cgtgcacgtg aacaggatgc cctacgacgg cgaagtggcc
     9781 ttcctggagt accttccggg gaagaagctg atggcattca accccaaggc gtcgcaggag
     9841 aacgagaggt tcttcatggg tttcgagacc ggatccggaa agggcatgac tgtccagata
     9901 gcgggcttcc tggcccggag gatagtctgc tcaaggaaga agggggaagt aatctctcgc
     9961 ggagagcgct tcggcatgat aaaattgggc tcaaaagttg acctgtacct cccggaaggg
    10021 atgatccccg ctgtaaaaaa gggggaaaag gtcagggcag gcgagacggt gatgggagtg
    10081 aggaaaaaag atgaggcgta agaacagagg gaagccggtt ccgatgaaga cgataatccc
    10141 caacatgata accagcggga atatgctctg cggcatgctc tctctggtcc tggcctttca
    10201 cgggcatttg gttccggcct catggctgat tttcatagcc gtgatcttcg atttcatgga
    10261 cggcaagatg gccaggaagc ttggaggtag cagcagcttc ggcatggagc tcgacagcct
    10321 tgccgacgtt gtcagttttg gtgttgcccc ggccatggtc atgtactctg tgtatctcaa
    10381 gggtttcttc ggagtaacgg gtgcgatcgt cgcggccttt ttcgcgctct gcggagcgct
    10441 caggctggcc aggttcaacg tacaccacgt ggacgggtcc ttccagggac tccccatacc
    10501 ggctgcagga cactttgtgg tctcctttgt cgttgcgggg atctatctcc ccccggccgt
    10561 tatggccgta atagttgctg tcacgggttt tctgatgata tccaacgtgt ccttcggcaa
    10621 cctcaaggca ctgaagcccg gtaacatgga ccgccggaag gcgaccttcc tttgggcttt
    10681 cgccttcatg ctcattgccg ttctcaggaa caaagccccg ctagcgggta tactaatata
    10741 cattgtcagt ggttttgcag gtgtggactg ggggaagtgg ctctcactgg aggctgtgga
    10801 cgaggaaaac ctggctgaga gagacgccta gggtcattac tgatcgcagg ggatggaggt
    10861 gccttatcga atgaatcatc tcattggcta cctggttgta ggggcgatcg cggggtggct
    10921 tgccggtaag gtctggcagg gaagggggtt cgggttcttc ggcaacatcg gggtcggagt
    10981 gatcggagcc ttcatcgggg gggtcatctt cagctccata gggatctacc ccatgggatt
    11041 tgtgggttct ctcgttatgg cctttgtggg tgccatagta cttcttttcc ttgtaggtgt
    11101 actgaaaaga aagtgatacc ccgaagacgg acgaacagag ggggccgcgg gagcggcccc
    11161 ctttttcatg atattttctc aagccttggc cagatcatgt atatggccac taccgacagg
    11221 actaggcttg ggagcatgaa cgagccgttg tagacagcag agtagatcca cacgttggta
    11281 ctttcaggcg cgtaggccgc aaagaagacc acgcctgaaa ggacatggca caggaaccgt
    11341 gccaaagccc cgagggccag cccggcccag agaggtctgc ggcagagtga tgccagcccg
    11401 agtacggaaa aggccatggg gtagtccagc agtgcctgta cagggtggac gacgtacccc
    11461 cccaggatga gctggagaag gcctgccacc gatcccgctc ccagaccagc cttcagaccc
    11521 cagcgtaaag tgaacaggaa aagggggacg ttctccaggg ttatggaccc accctggggc
    11581 atcctccaga tcctcacata cgacagggcc agcgccagcg ccgctgccat ggcgccctca
    11641 accaggatcc ttactttcct gttattcatt gcttccttct gtctcccttc ccttggtgat
    11701 cgaagagaac ctgtcctggt ccgaagaaaa acgccccccg gggttccggg gggcgtacgg
    11761 ggcaacttcc tgctactgtc ccgcaatatc ccttcgccgg cattacccgg atcaggttcc
    11821 aggggtcgag acctgcgtct cctctcagcc ggatcgttcc gactccccca ttgcagttct
    11881 ctttatacgg cggggtggca ggtttgtcaa acaggatcgg catgagaaat ggctattgtg
    11941 gtatcgtcat ctcctccgaa aggcttcttt cttcccgttg caggtagtgt tatattgaaa
    12001 aaaaaaggaa tggacctgta tccatcaaga ggtgatcttc ctgataactt tcttctgtcc
    12061 caggtgcggc agcattttcg acggaacggt aaggcccgtc ggctgcagtt gcggtgctcc
    12121 cctgaagagc tccatcgact ggaccgacct ggacatcgac cagtccgctc cgggtatggc
    12181 ccgttacgcc tctgccctgc ctccccttga ccccgctgtt tccatgggtg agggatggac
    12241 acctattgtg cgctcgacca ccggaggccc tttttataag ctggaatatc tttccccttc
    12301 gggttctttc aaggacaggg gctcggtcct ggtcatgacc gaggcaaagg cccttggatg
    12361 cggatcggtc atacaggact cctccggtaa tgcaggggca tcgatcgccg cctatgctgc
    12421 caggtgcgga ataaaagcga cagtaatagt gcccagggat atcaccgaag ccaagaagag
    12481 gcagatagag ctctacgggg ccgtccttga ggtcgtggag ggagacagga agactgcagc
    12541 ctcgctggcg ctctcgctat cggacagcac ctattacgca agtcacgtct ggaaccctct
    12601 cttcctggag ggaacaaaga ctgtggcctt cgagatatgg gagcagctcc atttcagggc
    12661 acccgatgcg gtggtagtcc cagccggaaa cggcacgatg ctgttggggg ttttcaaggg
    12721 ttttaaagag cttcttcagc gcgtcaggat caaaaggttg ccaaggatct atgccgtgca
    12781 gggcagccat tgcgcccctc ttcatgccca atggaacgag acgggagaga aggtcttcgg
    12841 gggcaccctc gccgaaggga tcgccgtgac ggatcccccg cgaatgaagg agatgctcga
    12901 tgcccttcgt gtgagcaggg gggatgtcct tgttgtcgag gacgagacga tccttttgga
    12961 acaggagcgt cttgccctgg aggagggcct gctggtggag ccgtcggccg cggtggctcc
    13021 tgccgcagag aggatgctcc ggggggcggg catgatatct tccggggaga ccgttgtggt
    13081 gcctctcaca gggagcgggc tgaaaaagat cccttccagg cgtcccaaaa aacaggcatc
    13141 ccctgatgag ggacgccccc tgttcgacaa cttgggtagg aagatcagag gttaagcctg
    13201 atccccaggc cgatccgctg ggccttctcg tagatgaccc tggcggtggc aacgtcgtgg
    13261 ctgccaatgc ccatgagtac cgccgccttt ctccccggtg tccttgggac ctcaacgatc
    13321 ccggcacaga tctcacccat gtcggcaggg acctcttcgg ggtaccccct gaaatatgtg
    13381 ccggccctct gggtctggag atactgcccc cggtcgtcgg tgacgaagaa agaggcgccc
    13441 gtcattacct cctcctcgaa agcggagtcg ttgttcgccg atacggcaag gacatccttt
    13501 ttcagccaat ctgccttgat gaacctgttg ggtttcctgt ggacgggggt acaggtaaca
    13561 atgacgtcag catccttgac ggcgctcctt gcatcggggc agagctcgat ctcggccccg
    13621 tggaccttgc tctgcatgtc ttccaggtac ttcctggcgt tggcctcgct tttgtcgtag
    13681 actgttaccc ttcggagtcc cttcatggtc tccctgagcc cgaggaggtt ggtccgcccc
    13741 tggactccca ggccgattat cgaaagagtg gaagcctttt caggggccag gtatttggca
    13801 gtgaccgcag tagcggcccc gctcctgtag gcagtgatcc actttgcgtc cataacggcc
    13861 aaaggggcac ctgtttcggc atcgttgatg acgataaggc ccattatgtt cggaagaccc
    13921 ttttctaggt tggaggggta acctgatacc catttaatgc ccaccgtgtc ggcggagcct
    13981 gcgaaatagg ccggcatcgc gtctaggaaa cagtcctccc tggggtagac gccctgtgag
    14041 ctgggaagct ggacaagccc ctcacccttg agctggaacc cttcctcaat aacttctatt
    14101 acctctctta tagggatcct catagattcg atctgttctc cgttgaggta gagcatgtcc
    14161 attgagatcg cctccctgaa aagtgcagta ttgagaattg tttgacctta ttatatctac
    14221 tttttccctg agacgagacc tggaatggta tggaaatgtc gggttgggta agtttatact
    14281 ggacgggctg ggacaaaaac agtatccagg ggagaggaag accgatgaga aaggccaggg
    14341 agatcgatgt gtcccttgtt tccgaggccg tcaagggtct cttcatcaag gccaacgtat
    14401 caatagggca ggatgtttac aatgccctgg tccaggccag agacaggatc gagggatccc
    14461 ccatcgggag atccctcgat cctgtctctg gcctggacca gggcatagac aggatcgagg
    14521 gatcccccat cgggagatcg gttttggacc agatcatccg gaaccacgat cttgcaagga
    14581 ccgaacagat gcccatctgt caggataccg gcatggccgt cgtcttcgct gacatagggc
    14641 aggatgtcct ccttacgggg ggagacctgt cagcggcggt caatttgggc gtcgaggagg
    14701 cataccggga aggttttttc cggaagtccg tcgttgagga ccctcttttc gaccggagga
    14761 acacgggcac gaacaccccg gccgtgatcc atacgaggat catcccgggg gacagggtca
    14821 agatatcagt gactccgaag ggtttcggca gcgagaacaa gagcgcactc agggtgttca
    14881 ctcccgccca gggaccgggg gaggtccggg attttatcag ggataccgta gcagctgccg
    14941 gtcctgatcc ctgcccgcca gtcctgattg gggtcggaat aggtggaacg gttgagtcgg
    15001 ccgcgataat ggccaaaagg gcaacactct atccggtagg acgcaggaac cctgaaagaa
    15061 gatacgcagc cctggaaatg gagaccctgg aggcagtgaa cgcccttgga ataggaccgg
    15121 ccggacttgg tggtaccgtc acctgcctgg acatccatat ggattttgct cccgggcata
    15181 ttgcgggtac ccccgtagtg gtcaacattt gctgccatgc ggcccggcat gcggaagtct
    15241 cgatctaggg aggtcccaat gatgagatat gcttcaagga tagtggcacc tctcaatgat
    15301 gaaatggtcc tctccctgcg ttccggagac aaggtcctta tcagcggggt catcttcacc
    15361 ggcagggatg cagctcacag acggatggct gaaaacatcg cggagaacag acctctaccc
    15421 ttcgatgtaa gggggcagat catctactac gtgggcccct cgccgacgcc cccgggaagg
    15481 gtctgcggct ctgctggtcc cacgacgagc ggcaggatgg acccctatac accactgctc
    15541 ctcgacctgg gcctgaaggg gatgataggc aagggcaagc gatcccctga cgtaattgaa
    15601 tcgatgaaga agaacagggc cctatacttt ggtgctgtcg gaggggctgc agtcctcatc
    15661 tccaaatgca tcaacaggat ggagacggtg gcgtggccgg aactgggacc tgaagccgtc
    15721 ttcaggatga gggtcgagga cctccccgtc gtggtactgg tcgactcttt cggaaacgat
    15781 ctttacagtg acggtcctgc cgggttccgt cgcagcgaat agtcactgtt tcaataaccc
    15841 ccggaaggga gggtccgatc tggaaggtgg aacagggtac ccccgcttct catggtgtgg
    15901 tgatgcctgc ctggtggccg agttcggcga taagatcgac cccctgatca actccatggt
    15961 cagggacctg gacctgagga taaggggtct taagcttcca tggctggtgg agactgtccc
    16021 cacttacagg tctctcgctg tctacgttga tccctgggat agcccccgtg aagaggttga
    16081 gcttgccgta acctctttgg cgcgggaggt gaaagcgaag accatgcctt ccgttgctgt
    16141 cgtcgagatc cccgtctgtt acggcggcga ctttggtccc gacctggaaa gggtctccat
    16201 gtacacggct cttggacctg atgatatcat ccggcttcac tccagcaaac tttaccgcgt
    16261 ctacatgatg gggtttaccc cgggcttccc ctacctgggt ggcatggacc cgcgacttga
    16321 aacgcccaga ctcgaggacc cgagaacact tattccggcg ggaagtgtcg ggatcgcggg
    16381 gaagcagacg gggatctacc ctatcgccag ccccggcggg tggaacatca tcggccggac
    16441 gccgctggtt ctctttgatc cggcgggagc cagtcctttc ctgcttgagg cggggatgga
    16501 gataaggttc gttcctgtga ccggggagag gttcgaggag atcctttcgg gaaatggagc
    16561 gtgacggctt gagcgtcgag attatctctc cgggagtgct gtctaccgtt caggatctag
    16621 gtcgttacgg gttccaggct tttggcatgc ctgtcgccgg cgcccttgac cgctactccc
    16681 tgatggtggg caaccttgtc gtaggcaatg acctccggtc agcagggctg gagataacca
    16741 tctccgggcc ggaactgctc ttccggtcgg aaaggctcgt ctgcataacc gggggagacc
    16801 tttctccgaa gatcaacgac cgtgacgtgc cggtctggca gggcctgatg ctgcacgaag
    16861 gagatgtgct gtcct
//
LOCUS       DAOPKQ010000007        15900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_3880_length_15900_cov_4.769580, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000007 DAOPKQ010000000
VERSION     DAOPKQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3880_length_15900_cov_4.769580"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PK146_00560"
     CDS             <1..423
                     /locus_tag="PK146_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPE90758.1"
                     /translation="VIDENNVITGESGILLVQGIDATADEIVDRARAFGGISILAHID
                     RPVYSYVAVLGMIPKDLEVDAVELSANLSEKEALQWLNETGGRPVIRSSDAHRIPDIS
                     IERTTPVLLKKPSFDELGLALKGIHGRRVLWPWGQDQT"
     gene            590..1060
                     /gene="nuoE"
                     /locus_tag="PK146_00565"
     CDS             590..1060
                     /gene="nuoE"
                     /locus_tag="PK146_00565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPE90759.1"
                     /translation="MTVQTTDVEGKVKAIIEPWKGKKGGLIPILQGVQHEFGFLQEDA
                     MEIISRELRIPTAEVYGVATFYAQFHLKPRGRHIIRICRGTACHVRGSLKILERVKEI
                     LKIEENGTTEDLRFTLEPVACLGACGLAPVVMINDVTYGRLTPDGIKPILDKHE"
     gene            1071..1655
                     /locus_tag="PK146_00570"
     CDS             1071..1655
                     /locus_tag="PK146_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE90760.1"
                     /translation="MLQDLSQYILDIAENSLKARASSVEIEVNEDTRENVLRLRVSDN
                     GVGLDSEQLSMVENPFFTTRTERRVGLGIPFLRQAADTCDGSFGIRSAKGRGTVIEAS
                     FRRDCIDCPPLGDIPATIMTLMVGWPERSFLFRFRFNDDIFETGTEELLQILEDRELL
                     ASAEVALWISRYMEQGIYNLRTGGNEGFEEDYQP"
     gene            1633..2004
                     /locus_tag="PK146_00575"
     CDS             1633..2004
                     /locus_tag="PK146_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPE90761.1"
                     /translation="MKKITSLEDLRRIKEQSKDLTSARSGGKTRIIVGMGTCGIAAGA
                     REVMTVILQELDRRGLKDVAVETTGCIGMCQKEPLVDVIRPGELRTTYGDVGPADVPR
                     IIVEHIVNGNIVEDKVVGQTD"
     gene            2017..3813
                     /locus_tag="PK146_00580"
     CDS             2017..3813
                     /locus_tag="PK146_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPE90762.1"
                     /translation="MALYRAHVLVCKGTGCTASGSGSVYDALKEELERRGLDKEVMLV
                     ETGCHGMCEMGPIVVVYPEGSFYCRVTPEDVPELVEEHLYKGRVLQRLLYTTPDEEKH
                     RIPHYRDIPFYGKQRRIVLRNCGYINPDNIEEYIARDGYQALARALLEFSPEETLDIV
                     KRSGLRGRGGAGFPTGKKWEFARMAPGDKKYIVCNADEGDPGAFMDRSVLEGDPHSVV
                     EGMLIGAYVIGSDEGYIYCRAEYPLAIKRLVTAIQQAEEMGLIGDNIMGTDFSFHLHI
                     KEGAGAFVCGEETALMASIEGKRGMPNPRPPFPANSGLWGKPTNINNVETWANIPQII
                     LNGAEWFSSVGTETSKGTKIFALTGKVNNTGLVEAPMGMSLREVIFDIGGGIPNGKKF
                     KAVQLGGPSGGCLPESMLDTPVDYESINATGAIMGSGGMVVTDEDTCIVDFAKFFLTF
                     VQNESCGKCPFCRIGTRRMLEILERICEGKGKMEDLDLLEDLAQKVKDGSLCALGGTA
                     PNPVLTTLKYFRDEYVAHIVDKKCPAKVCPALIQYTIDPAKCIGCTKCARQCPVNAIS
                     GEIKKPHLIDQDTCIKCGQCMAVCPVKAISVE"
     gene            3830..7933
                     /gene="fdhF"
                     /locus_tag="PK146_00585"
     CDS             3830..7933
                     /gene="fdhF"
                     /locus_tag="PK146_00585"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:6299..6301,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPE90763.1"
                     /translation="MDRDVKVILNGKEVFGYRGQKILELCAENGIEIPTLCYDPHLTL
                     HGGCSLCLVEVKGARNLVRACAMPINNGMEIQTMTDRVYGARQLALQLLLSDHVGDCR
                     PPCQLACPARGDVKGYVNYAAEGEYRASLEALHDNITLPASIGRICPAPCEEKCRRNF
                     VDEEPVSIREIKRFIGDWGIASGDLGNIPEIKENGKKVAIVGGGPAGVSAAYYLRRMG
                     YAVTLFDKEPALGGMMRYGIPDYRLPQPILQKEIDWILAHGIETRLDTALGRDVTLDE
                     LRKSYDSVLLAMGCWKSMPMRVPGEDLKGVLGGINFLYEVNNGRKVDLGSKVAVIGGG
                     NTAMDAARCALRYGAGKVYVVYRRTREEMPAEKMEIDEAMEEGVEFIFLAAPVAVEGN
                     GKVERIVCEKMELGEPDASGRRRPVPTGEQFTLEVDTVIAAIGQGADLTPLPGEMHDG
                     RKVSSDKHFATPMEGVFVCGDLNTGPDIAVAAIGEGHWAAESINHFIQEGVPHRPFEC
                     DVVRDDLGPEDFLDEEKQPQEHPVHCPGDVRLSRPFEEYNLGLSEEQVLRDGSRCMKC
                     GCADVHECKLRKFGTDYEADPETFPKGEYSRPQRTVEANKYYLRNMDKCISCGVCVRA
                     CSQQAIYSAIDFQHRGMKTLIAPALDRGIEDSTCVFCGQCVQLCPTGALTENSVHGIS
                     RPSRSRVVKTICSYCGVGCELNIHVDELTGKIWNVTTDYDSATSMNNGRCCVKGRFAW
                     SFVHSEDRLRKPLIKEKGVFREASWDEALGLVAKKFTGFRDSSGPDSLAFFTSARCTN
                     EENYLLQRFAREVMGTNNVDHCAHLUHSPTVAGLGETLGSGAMTNDYESIRHADVALV
                     IGSNTTENHPVIGSWLKEQQKNGKTSLIVCDPRRIELDDFADIKVRHRSGSDVALLNG
                     LMNIIISEGLYESEFVEKNVEKFDELKNLVAEYTPGKVSAITGVPEQTLKEAARTYAK
                     GPNSAIFYTMGITQHKYGTNNVKTVANLALLCGMLGRPGTGVNPLRGQNNVQGACDMG
                     CLPALLPGYLNIAKSTEQAVEKVKKLWNAKLPTAPGRSIVKIIEGAASGEIKGLFIMG
                     ENPMVSDPDTNHVRHALENTEFLVVQDIFLTETAQLADVVFPATCWAEKDGTFTNTCR
                     AVQRIRKAVNAPGEARPDWEIILDLAKACGAKWNYSSPADVMEEISRFVPQYGGVSYD
                     RLDQGPLMWPCPDKDHPGTPNLYTTGFPRGKATFVPCEWNEPHEWPDAEYPFLATTGR
                     SLYHYHTGTMTRRTPSAEFIKSLYIEVNPEDAAAMGLGEGDDVKVTSRRGSVVGEARI
                     TDRVPPKMVFVPFHFGEQPANALTASVWDISTETPAYKINAVKVEKA"
     gene            complement(8020..10101)
                     /gene="fusA"
                     /locus_tag="PK146_00590"
     CDS             complement(8020..10101)
                     /gene="fusA"
                     /locus_tag="PK146_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPE90764.1"
                     /translation="MATRDPENIRTVALVGHGGSGKTALAEAIAFNTGLTTRMGKVED
                     GNTVSDFGNEEQKRQISINSSLVSIEHAGRNVYIIDCPGYADFIGELRSSMRVVDSAL
                     VVISGVHGVEVQTEKAWEFGEDMQIPMAFYISKMERENADFERTLSEIQDYLSDKAVP
                     LFLPVGKEAAFKGVVDVLRQKAYMYKGDGSSEFNETDVPSDLADYVTSAREVLVERIV
                     EADDELMMRYLEGEQLSMEELVPALRKSVLSRSLFPVLPGASTSNVGVCQLLDAIAEV
                     LPGPLDMPPRKALKGDSEVMVEPDPAGKFSAFCFKIMVDPYVGKLSFIRVFSGSASSE
                     NPLYNLSRGEDERVSAFKVMRGKESVDTRDVVVGDIVAIPKLHSTVVGDTLGVKGMDF
                     IYPEIQFAKPVFSVAVRPRSRADEDKLANALNKMLEEDQTLHFEKNPETNDSVLSGMG
                     DLHIDVMLSKIKERYGVELDTETPKVPYRETIKKPSKAQGKYKKQTGGRGQYGDVHIE
                     FVPLSRGDGFLFEDKIVGGAVPKSYIPAVEKGLREALQKGVLAGYPSVDFKASLFFGS
                     YHDVDSSEMAFKIAASMAFKKGMVEASPVLLEPIMNVEVVVPEDYLGDVMGDFNGRRG
                     KIMGIDSRGRLQVVKAQVPLAEMFRYAIILRSMTSGRGNFTMEFSHYEEVPHDIAKKI
                     IAEAEVQEDED"
     gene            complement(10252..11736)
                     /gene="gatB"
                     /locus_tag="PK146_00595"
     CDS             complement(10252..11736)
                     /gene="gatB"
                     /locus_tag="PK146_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPE90765.1"
                     /translation="MTPSFETVIGLEVHVQLATATKLFCSCPTDYIGSEPNTLVCPVC
                     LGLPGTLPSLNGRSVELGVKTALALGCSISRTSRFHRKNYFYPDLPKAFQISQYDLPV
                     ATGGSMPVFVGGEKKFIGITRLHLEEDAGKLVHGTADGRLSGRSFSNVDYNRAGIPLA
                     EIVSEPDMRSPEEAREYVLTLRRLVRYLGVSDGDMESGSLRVDANISQKCSDGRWGGR
                     VEIKNVNSVRAMERALDFEITRQRGIMLSGGTVVQETRHWDDSAGVTRSSRGKEEAHD
                     YRYFPEPDLPPLIVNDALVRAMGESLPELPWVKRERFETEWGLSPEDAAVLSERLDLA
                     EFLESTVASGAGRDIAANWIKTEILRILNETGLDAGDLPFTPRVLGELLGEVVKGSLS
                     NTAARDVLEVMYRKGLGIQDAMKETGVVPGGVTGDLLAKAVRRVLDANREETGLVLSG
                     ADPSGKKRKYLQGLVMREIRGQAPVGDVSAVIDEALRQMRGLDE"
     gene            complement(11736..13205)
                     /gene="gatA"
                     /locus_tag="PK146_00600"
     CDS             complement(11736..13205)
                     /gene="gatA"
                     /locus_tag="PK146_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPE90766.1"
                     /translation="MKHEQMDASSIASAVTSGEVSAVEVVSASLRRIRSLEPRLNALI
                     TVIEEYALERAAHVDGQVSSGCDPGPLAGVPVVVKDNMCTRGVRTTCGSRILDDWEPP
                     YDAAVVELFERAGAVIVGKSNMDEFAMGSSTEHSCFGPASNPRDISRVPGGSSGGSAA
                     SVAAGYVPIALGSDTGGSIRQPAAFCGVHGLKPTYGMVSRWGLVAFASSLDQIGPFGR
                     SVGDIALAMDVISPQDFRDSTCSRRERPSFRDGASNPAVKGMRIGIVREFLDGPLDPS
                     LKRSIEEAASVLVSSGCTMVEVSLNESASFGLPCYYIIAPAEASSNLARFDGVRYGFR
                     EEAPDLVSQFTRTRSYGFGEEVKRRILTGTFVLSSGYYDAYYLTALKARKMICQEFDR
                     AFRSVDVILMPAVPSLPFRKGELLDDPISMYMSDVFTLPVNLAGLPGMTVFTGLEPSG
                     LPTSVQLVAPRWQDRRLVECASVIEERFGCPPIAEGGAL"
     gene            complement(13195..13503)
                     /gene="gatC"
                     /locus_tag="PK146_00605"
     CDS             complement(13195..13503)
                     /gene="gatC"
                     /locus_tag="PK146_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPE90767.1"
                     /translation="MEIDRKIVERAATLSRIEMVPDNVETMVRFFQDIIGHFRILEKC
                     DTTGVDPFSTLEDEPCPLRGDGPVRWDIGDEALDSAPLREGRFFRVPAIGSEEGPDEA
                     "
     gene            complement(13505..15562)
                     /gene="ligA"
                     /locus_tag="PK146_00610"
     CDS             complement(13505..15562)
                     /gene="ligA"
                     /locus_tag="PK146_00610"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPE90768.1"
                     /translation="MGDQEIRERIGRLREEIKRHNYLYYVLDSPEISDDRYDLLLREL
                     KELEALTGEQPPPDSPTATVGGAPRNEFVKVVHDSPMLSLENAFSREDVEDFLRKVSK
                     GAAYPSLCCELKIDGLAVSLHYNEGKFILGATRGDGRTGEVVTRNLLTISDLPRDLAG
                     KVPGRLEVRGEVLIKSEDFMALNASREEQGLPLFANPRNAAAGSLRQLDPSVTASRRL
                     SLFVYQVVSPGDHGLSSQFRSLSWLRKMGFPVQGTECLCRGAEEAFSYIERWRNERFS
                     LPYVTDGVVIKVDDLGAWSGLGTTAKVPRWAVAYKYPPEEKLTRLVRIEVSVGRTGTL
                     TPIAILEPVALSGTVVQRASLHNADEVARKDIREGDMVWVRKAGEIIPEVLRPDLASR
                     KESSVPFRMPSRCPACGSDAISLPGEVALRCVNRSCPAQILEGLRHFASRSGMDIGGL
                     GEKLLEKLVEEGVVSDIADIYSLSMGDLAPILLTGEKSSRVLGEKAASSVLDSISSSK
                     ERPLSALIAALGIRYVGSRVAEILAEAFGSLEDLGSCDEDSLASIEGVGPRIASSVAA
                     FFRDPRNRETIARLKAAGLNVSSSGSPQRGGVGPLKGLSFVFTGELEGMTRSEAENRV
                     KELGGSTPSSVSRKTSFVVSGSAPGSKLAKAVTLGIRVIDEKTFLDLIERATGGERPE
                     VER"
     gene            complement(15588..>15900)
                     /locus_tag="PK146_00615"
     CDS             complement(15588..>15900)
                     /locus_tag="PK146_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPE90769.1"
                     /translation="KNPEDPDFTNRFELYIYGSEVANAFSELNDPLDQRERFMDQLKK
                     KEAGDDEAHVFDEDYINALEYGLPPTGGLGIGIDRLAMFLTDSRSIRDVILFPTMRPK
                     V"
BASE COUNT         3541 a         4663 c         4509 g         3187 t
ORIGIN      
        1 gtcattgacg agaacaacgt cattaccgga gagtccggca ttctcctggt tcagggcatt
       61 gatgccaccg ccgacgagat cgttgacagg gcaagagcct tcggaggaat aagcatactg
      121 gcccacattg accgcccggt ctactcctac gtggcggtcc tggggatgat ccctaaggat
      181 cttgaggttg atgcagtaga gctctcggcg aacctgtccg agaaggaagc cctccaatgg
      241 ctcaatgaga ccggggggag gccagtgata aggtcctccg acgcccaccg catcccggac
      301 atcagcattg aaaggacgac gccggtgttg ctcaaaaaac cgtccttcga cgagttgggt
      361 cttgccctca agggcatcca cgggaggagg gtcctctggc cctgggggca ggatcaaacc
      421 tgacatcttc catacataca caatggacga agggagaacg tgcccatggg cctttaggat
      481 tctgttccat tctgttcagt aaagtttgca ataaacccag aaagtgatat tattcacctg
      541 cctttcagga acagcaagac acgaaaaatc tattcaggtg gtggtctttg tgacggtcca
      601 gacaacagat gttgaaggaa aggtcaaggc gataatcgaa ccctggaaag gcaaaaaggg
      661 aggcctgatc ccgatcctcc agggtgtcca gcacgagttc gggtttctcc aggaggatgc
      721 catggagatt atctccaggg agctcaggat accgacagcc gaggtctatg gcgtcgctac
      781 cttttacgcc cagttccatc tcaagcccag ggggagacac attatcagga tatgccgggg
      841 tacggcctgc cacgtaagag gaagcctgaa gatcctcgag agggtcaagg agatactcaa
      901 gatcgaagag aacgggacca ccgaagatct tcgcttcacc ctcgaacctg tcgcgtgtct
      961 aggcgcatgt ggcctggctc ccgttgtaat gataaatgac gtgacctacg gaaggcttac
     1021 ccccgacggg atcaagccta tcctcgacaa gcacgaatag acacccgacc atgcttcagg
     1081 acctctccca gtacattctc gatattgcag agaattccct gaaggcccgt gcatcaagcg
     1141 tagagatcga ggtcaacgag gataccaggg agaacgttct tagactcagg gttagcgaca
     1201 acggggtggg gctggacagc gaacagctct ccatggttga gaatcccttc tttaccaccc
     1261 gtaccgaaag aagagtcggg ttaggaatac ccttcctcag acaggctgca gatacctgcg
     1321 acggaagttt cgggatacgt tcagcaaagg ggaggggcac cgtcatcgaa gccagtttca
     1381 gaagagattg catcgattgt cctcctctcg gggatattcc ggctacgatc atgaccctca
     1441 tggtcgggtg gcccgagagg tcatttttgt tccgcttcag gttcaacgat gacatcttcg
     1501 aaacaggcac agaagagctg ttgcagatcc tggaagacag ggaattattg gcttctgcgg
     1561 aagtcgcact atggatctcc cgctacatgg aacagggtat ctacaatctc cgaacagggg
     1621 ggaatgaagg ttttgaagaa gattaccagc cttgaggatc tgagaaggat caaggaacag
     1681 tcgaaggacc ttacttcggc ccgttccggc ggcaagacaa ggatcatagt tggaatggga
     1741 acgtgtggca tagcggcagg cgcaagggag gtcatgacgg tcatccttca ggagctggac
     1801 aggagagggc tcaaggatgt ggctgtagag accaccggtt gcataggcat gtgccagaag
     1861 gaaccgctgg tggacgttat ccgtccgggc gagctccgca ccacctatgg cgacgtcgga
     1921 cccgccgatg ttcccaggat catagtcgaa cacattgtca acggcaacat tgtcgaggac
     1981 aaggttgtcg gacagactga ttagggggct ctgaagatgg ctttgtatcg cgcccacgtt
     2041 ctggtttgta agggtacagg ttgcacggca agcggctcag gctccgttta cgacgccttg
     2101 aaggaggaac tggagcgtcg tggactggac aaggaggtca tgctggtcga gacagggtgt
     2161 cacgggatgt gcgaaatggg acccatcgtc gttgtctacc ctgagggctc cttctactgc
     2221 cgggtgaccc cggaggatgt tccggagctt gtcgaggagc atctttacaa gggcagggtc
     2281 cttcagaggc ttctttacac aacccctgac gaagagaaac acaggatccc ccactacaga
     2341 gatattcctt tctacggaaa gcagcggagg atagttcttc gaaattgcgg ttacataaac
     2401 ccagacaaca ttgaggagta tatcgccagg gacggatacc aggcgctggc cagggccctt
     2461 ctcgagtttt ccccggagga gaccctggac atcgtcaaga gatccggtct gagggggcgc
     2521 gggggagccg gattccccac gggcaagaag tgggagttcg ccaggatggc cccgggggac
     2581 aaaaagtata tcgtatgcaa cgccgacgag ggcgaccctg gtgctttcat ggaccgttcg
     2641 gtccttgagg gtgatcctca cagcgtggtc gaagggatgc ttatcggagc ctatgtcatt
     2701 ggttccgacg aaggttacat atactgcagg gcagaatacc cactggccat caagcgcctg
     2761 gtgaccgcca tccagcaagc cgaggagatg ggcctcatag gtgacaacat catggggacg
     2821 gacttctcct tccatctcca catcaaggag ggcgcaggcg cattcgtctg cggggaggag
     2881 acagctctca tggcctccat cgagggcaag cgcggcatgc ccaacccgcg tcccccattc
     2941 ccggccaact cgggactttg ggggaagccg accaatatta acaacgtgga aacatgggcc
     3001 aacataccgc agataatcct gaacggcgcc gaatggttca gcagcgtcgg gacggagaca
     3061 tcgaagggga ccaagatatt cgctctcacc ggcaaggtca acaacaccgg cctcgtcgag
     3121 gcacccatgg ggatgtctct cagagaggtc atctttgata tcggcggcgg cataccgaac
     3181 ggaaagaagt tcaaggcagt acagcttggc ggtccttccg gagggtgcct gccggaatcg
     3241 atgctcgaca cgcctgtcga ttatgaatcg atcaatgcta ccggcgccat tatggggtcg
     3301 ggaggaatgg tcgtaaccga cgaagacacc tgtatcgtgg acttcgccaa gttcttcctg
     3361 accttcgtcc agaacgaatc ctgcggcaag tgccccttct gcaggatcgg gaccaggagg
     3421 atgctggaga tcctggaacg catctgcgaa ggcaagggga agatggaaga ccttgacctt
     3481 ctggaggatc ttgctcagaa agtcaaggac ggctccctct gtgccctggg ggggacggcg
     3541 cccaacccgg tgctgaccac tctcaagtac ttccgtgatg agtacgtcgc ccacatcgtc
     3601 gacaagaaat gcccggccaa ggtctgtcct gccctgatac agtacaccat cgacccggcc
     3661 aagtgcatcg ggtgcaccaa gtgcgcacgc cagtgccctg tcaacgcgat cagcggcgag
     3721 atcaagaagc ctcatctcat tgaccaggac acctgcatca agtgcggaca gtgcatggcg
     3781 gtatgcccgg tcaaggcaat aagcgttgaa taaggaggat ctttgaacga tggatcgaga
     3841 cgtaaaagtt atccttaacg ggaaagaggt cttcggctat cgaggccaga agatcctgga
     3901 gctatgcgct gagaacggca tagagatacc cacgctctgt tacgacccgc acctgaccct
     3961 tcacgggggt tgttcccttt gcctggtcga ggtgaaggga gcgaggaacc tggtcagggc
     4021 atgcgccatg cctattaaca acggcatgga gatccagacc atgaccgacc gggtctacgg
     4081 agccagacag cttgccctgc aacttctact ctcagaccac gtgggcgact gtcgcccccc
     4141 gtgccagctt gcctgccctg ccagagggga tgtcaagggc tacgtcaact acgcagctga
     4201 aggcgagtac cgcgcatctc tcgaagcgct ccacgataac atcaccctcc cggcctctat
     4261 cggccgaatc tgccctgcgc cttgcgagga gaagtgccgc aggaacttcg tcgacgaaga
     4321 acccgtctct attcgtgaga tcaagaggtt catcggggac tggggcatcg cctccggcga
     4381 cctgggaaac atccccgaga tcaaggagaa cggcaagaag gtggccatag tcgggggggg
     4441 gcctgctggc gtatctgccg cctattacct gagaagaatg ggctacgctg taaccctttt
     4501 cgacaaggaa ccggccctgg gtggaatgat gaggtacgga atacccgact accggctccc
     4561 acaacctatc ctgcagaagg agatcgactg gatcctcgcc cacggcatcg agaccagact
     4621 tgacactgcc ctcggcaggg acgtgaccct ggatgagtta aggaagagct acgattcagt
     4681 cctgcttgcc atgggatgct ggaagtcaat gcccatgaga gtccccggag aggacctcaa
     4741 gggggtcctg ggcggtatta acttccttta cgaggtcaac aacggacgca aggtcgatct
     4801 tgggagcaag gtggctgtca tcggcggcgg gaacaccgcc atggacgcag ccaggtgcgc
     4861 cctccgctac ggcgccggaa aggtttacgt ggtctaccgc aggacccgcg aggagatgcc
     4921 cgcggagaaa atggagatcg acgaagcgat ggaagaggga gtggagttca tcttccttgc
     4981 tgccccggtg gcggtggaag gtaacggtaa ggtcgaaagg atagtctgcg agaagatgga
     5041 actcggcgaa cccgatgctt caggccgcag aagacctgtc cccaccggtg agcagttcac
     5101 cctcgaagtg gacacggtga ttgcagccat tggacaggga gccgacctta cccctctccc
     5161 cggtgagatg catgacggca ggaaggtcag ctccgacaag cactttgcga caccaatgga
     5221 gggggtcttt gtctgcggcg acctcaacac cggccccgat atcgccgttg ccgcaatagg
     5281 cgaaggccac tgggccgccg aatcgatcaa ccacttcatc caggaaggcg taccacacag
     5341 acccttcgaa tgcgacgtcg tacgggacga tctcgggcca gaggacttcc tcgacgagga
     5401 gaaacagcca caggagcacc ccgtccactg tccgggagat gttcgcctgt caaggccctt
     5461 cgaagagtac aacctggggc tttccgagga gcaggtgctc agggatggaa gccgctgcat
     5521 gaagtgcggc tgcgcagatg tccacgaatg caagctgagg aagttcggga cagattacga
     5581 ggcagatccg gagaccttcc cgaagggtga gtactctaga ccccagagga cggtcgaagc
     5641 caacaagtat tacctcagga atatggacaa gtgcatctcc tgcggggtct gcgtaagggc
     5701 atgcagccag caggccatct acagcgccat cgatttccag cacaggggga tgaagaccct
     5761 gatcgcgccg gccctggacc gggggataga ggattcgacc tgcgtcttct gcggccagtg
     5821 cgtacagctc tgccccacag gagcgctgac ggagaactct gtccacggca taagccgacc
     5881 gtcaagatca agggtcgtca agaccatctg ctcctactgc ggggtcggct gcgagctgaa
     5941 catccatgtt gacgagctca ccggaaagat atggaacgtt accaccgact acgacagcgc
     6001 cacctctatg aacaacggga gatgctgcgt gaagggccgg ttcgcctgga gtttcgtcca
     6061 cagcgaagac aggctcagaa aacctctgat aaaggagaag ggcgtcttcc gggaagcatc
     6121 ctgggacgag gctctgggtc tggtggcgaa gaagttcacc gggttcaggg attccagcgg
     6181 ccccgattcg ttggcgttct tcacctctgc gcgctgtacc aacgaggaga actacctgct
     6241 ccagcgcttc gcaagggagg tgatgggcac taacaacgtc gaccactgtg cccatctctg
     6301 acacagtccc acagttgcgg gtctcggcga gaccctcgga agcggagcaa tgaccaacga
     6361 ttacgagtcc atcaggcacg ccgatgtcgc cctggtgata ggatccaaca cgacggagaa
     6421 ccaccccgtg atagggtcct ggctcaagga acagcaaaag aacggcaaga ccagcctgat
     6481 cgtatgcgac ccgagaagga tagagctcga cgactttgcc gacatcaagg tgaggcatcg
     6541 ttcagggagc gacgtggccc ttctcaacgg gctgatgaac attatcatca gcgaaggtct
     6601 ctatgaaagt gagttcgtcg agaagaacgt cgagaagttc gatgaactca agaacctcgt
     6661 tgccgagtac actcccggca aggtaagcgc catcaccggc gttcccgaac agaccctgaa
     6721 agaagctgca aggacctatg ccaagggacc gaactcggcg atcttctaca ccatggggat
     6781 cacccagcac aagtacggaa cgaacaatgt caagaccgtg gccaaccttg ccctgctctg
     6841 cggaatgctc ggaagacccg gaaccggcgt caacccgctg aggggacaga acaatgtcca
     6901 gggcgcctgt gacatgggct gtctgcccgc gctgctcccc ggctatctca acatagccaa
     6961 gagcaccgaa caggccgtcg agaaggttaa aaagctctgg aacgccaagc tgccaacggc
     7021 gcctggcaga tccatagtca agatcataga gggagccgcc agtggcgaga tcaagggact
     7081 cttcataatg ggagagaacc ccatggtcag cgacccggac acgaatcacg tcaggcatgc
     7141 ccttgagaac accgagttcc tcgtggtcca ggacattttc ctgaccgaga cggcccagct
     7201 ggcggacgtg gtattccctg cgacctgctg ggccgaaaag gacgggacct tcaccaacac
     7261 ctgcagggcc gtccagagga tccgcaaggc cgtaaacgca cccggagagg cccggccgga
     7321 ctgggagatc atcctggacc tggcaaaggc ctgcggggcg aagtggaact acagctcacc
     7381 tgcagatgta atggaggaga tcagtcggtt cgtaccccag tacgggggcg tttcctacga
     7441 caggctggac cagggtccgt taatgtggcc ctgcccggac aaggaccatc ccgggacacc
     7501 aaacctttac accaccggct tcccccgggg caaggccacc tttgtaccat gcgagtggaa
     7561 tgaaccccac gagtggcccg acgccgagta ccccttcctg gccaccactg gaaggtcgct
     7621 ctaccactac cacactggca ccatgacgag aaggacccca tccgcggagt tcatcaagtc
     7681 gctgtacatt gaggtcaacc ctgaggacgc ggcggcaatg gggttgggcg agggagacga
     7741 tgtgaaggtc acatcgaggc gtggcagcgt agtaggcgaa gcaaggatca cagacagggt
     7801 cccgccgaag atggttttcg tgccgttcca cttcggagag cagcccgcta atgcccttac
     7861 ggcctcggtc tgggacattt ccacagagac cccggcgtac aagatcaacg ccgtgaaggt
     7921 cgagaaagcc tagaagggat ctgcatctac aatgaaacgg ggcccgggat catcccgggc
     7981 cccgtttttt tggactgggt cttcccaagg cattgcagac taatcttcgt cctcctggac
     8041 ctctgcctcc gcaatgatct tcttggctat gtcgtgggga acttcctcgt agtgggagaa
     8101 ctccatggta aagtttccgc gccctgaggt cattgatctc aggattatgg cgtaccggaa
     8161 catctcggca agagggacct gggcctttac gacctggagc ctccccctgc tgtctatccc
     8221 catgatcttg ccccttcggc cgttaaagtc tcccatgacg tcccccaggt aatcctcggg
     8281 aacgaccacc tcaacgttca ttatcggctc cagcagtaca ggagaagctt cgaccattcc
     8341 cttcttgaag gccatggaag cggctatctt gaaggccatc tccgacgagt ccacatcgtg
     8401 ataggaaccg aagaagagac tggccttgaa gtccaccgat gggtagcctg caaggacccc
     8461 cttctgcagg gcctctcgaa gccccttctc cacggcaggg atgtaggact tgggcactgc
     8521 cccgccgacg atcttgtcct cgaacaggaa gccatcaccc cgggagagtg gaacgaactc
     8581 aatgtggaca tcgccatact gtccccttcc cccggtctgt tttttgtact ttccctgggc
     8641 cttggagggt ttctttatgg tctccctgta gggaaccttc ggagtctccg tgtccagttc
     8701 aaccccgtac cgttccttga tctttgagag catgacgtct atgtgcaggt cacccatgcc
     8761 tgaaaggaca ctgtcgttgg tctccggatt cttttcgaag tgaagggtct ggtcctcctc
     8821 gagcatcttg ttcagggcgt tagcgagctt gtcctcgtcg gcccggctcc tgggtctgac
     8881 cgccacgctg aacacgggtt tggcgaactg gatctcgggg tagatgaagt ccatgccctt
     8941 gacccccaga gtgtcgccca caacggtgct gtgcagcttc ggtatggcca caatgtcccc
     9001 aacaaccaca tccctggtat ccaccgattc cttgcccctc atcaccttga aggcgctgac
     9061 ccgctcgtcc tcaccccttg ataagttgta gagggggttc tcgctggagg ccgaaccgga
     9121 gaataccctg atgaaggaca gcttccctac gtagggatcg accatgatct tgaaacagaa
     9181 ggctgagaac tttccggcgg gatccggttc taccattact tccgagtcac ccttgagggc
     9241 tttcctggga ggcatatcca gcgggccggg aagcacttct gcaatggcgt cgaggagctg
     9301 gcagacgccc acgttggaag tactggcccc tgggaggacc gggaagagcg acctggaaag
     9361 aaccgacttt ctcagggccg ggacaagctc ctccatggag agctgttcgc cttcaagata
     9421 tctcatcatc agttcgtcgt cggcctcgac tatcctctcg accaggacct cccttgcaga
     9481 ggtgacgtag tcggccaggt cggagggaac gtcagtctcg ttgaactcac tgcttccgtc
     9541 gcctttgtac atgtacgcct tctgtctcag aacatccacg acgcctttga aggccgcctc
     9601 cttgcctact ggaagaaaca atggcacagc cttgtcggag agatagtcct ggatctcact
     9661 gagggtcctc tcgaaatcgg cgttctctct ctccatcttg ctgatgtaga aggccatggg
     9721 gatctgcatg tcctcaccga actcccaggc cttctcggtc tgtacctcca cgccgtggac
     9781 accgctgatc acaaccaggg cagaatcgac caccctcatt gacgacctca gttcgccaat
     9841 aaagtcagcg tagccggggc aatcaatgat gtagacattc ctgccggcat gctctatcga
     9901 aaccagcgac gagttgatcg atatctgtct cttctgctct tcgtttccga agtcgcttac
     9961 ggtgttgcca tcctccacct tccccatcct ggtggtaaga cccgtattga aggctatggc
    10021 ttcggccagg gcggtcttgc cggaaccacc atggcctaca agggccacag tccttatgtt
    10081 ctcaggatcg cgagttgcca tctactgata cctccctcga gtgattaata cttttattcc
    10141 agccattctc tttacgctgc atgaaacaaa acagccttcg agatcaacgc acgcaggaat
    10201 aatcttaagt ggcagggagt gggccgtcaa tgagcacgac aatggccggt ctcattcatc
    10261 caggcccctc atctgtctca gcgcttcatc aattacagcg gacacatccc ctacaggggc
    10321 ctgaccccgt atctccctca tcaccagtcc ctggaggtac tttctcttct tgcctgaggg
    10381 atcggcaccg gaaagcacca gacctgtttc ttccctgttg gcatcaagga ccctcctgac
    10441 agctttcgca agaagatccc ctgttacccc gccggggacc actcctgttt ccttcatcgc
    10501 atcctggatc ccaagaccct tgcgatacat aacctcaagg acatcccggg cggccgtatt
    10561 tgacaaagat cccttgacga cctcacccag gagctctccc agaacccggg gtgtgaaggg
    10621 aagatcccct gcgtccaaac ctgtttcgtt aaggatcctc agtatttcgg tctttatcca
    10681 gttggcagct atatccctcc cggctccgga ggcaacggtg ctctcaagga actcggcaag
    10741 gtcaagacgt tctgagagga ccgctgcgtc ctcggggctc aaaccccact ccgtctcgaa
    10801 acgctctctc ttgacccagg ggagctccgg gaggctctcc cccatcgcac ggacaagggc
    10861 atcattgaca atcaaaggtg gaagatcggg ctccggaaag tagcgatagt cgtgagcctc
    10921 ttccttcccc ctggaggacc tggtgacccc agccgagtca tcccagtgtc gggtctcctg
    10981 gaccaccgtg cctccggaaa gcatgatacc cctctgtcgg gttatctcga aatccagggc
    11041 tctctccatg gccctgacgg agttgacgtt cttgatctcc acccttccgc cccacctccc
    11101 atctgaacat ttctgggaga tgttggcgtc caccctgaga gagcctgatt ccatgtcccc
    11161 atcggagact cccaggtacc tcacgagcct gcggagcgtc agcacgtact ccctggcctc
    11221 ctcgggagag cgcatatccg gctcggagac gatctccgcc agggggatcc ctgcacgatt
    11281 gtaatccacg ttggagaagg accttccgga aagcctgccg tcggcggtcc cgtggacaag
    11341 tttgcccgcg tcctcctcga ggtggagcct cgtgataccg atgaactttt tctccccccc
    11401 gacaaagaca ggcatcgaac ctccggttgc gacaggaagg tcgtactgac ttatctgaaa
    11461 tgccttgggg aggtctgggt agaagtagtt cttcctgtga aacctggagg tcctcgaaat
    11521 ggaacacccc agggcaaggg cagtcttgac acccagttcg acggatctgc cgtttaaaga
    11581 gggaagggtt ccgggaagac cgaggcagac ggggcagaca agggtgttgg gttcagaccc
    11641 gatatagtct gtcgggcatg aacagaagag cttcgtcgcc gtggcgagct gcacatggac
    11701 ctccagtcct atgacggtct caaaggacgg tgtcatcaca gggcacctcc ttccgcgatc
    11761 gggggacatc cgaaccgttc ttcgatcacg gaagcgcact ctacaagtct gcggtcctgc
    11821 catcggggag ccacaagctg tacgctggtc ggcaggccag aaggttctag tccggtgaag
    11881 actgtcatcc ctggaagccc cgcgagattg accggtaagg tgaagacatc gctcatgtac
    11941 atacttatgg ggtcgtcaag tagctcccct ttcctgaagg gaagggacgg aacggcaggc
    12001 attaggatca catcgacgga cctgaaggcc cggtcaaact cttgacatat catcttcctt
    12061 gccttcaggg ctgtgagata gtaggcatca tagtaacccg aactcagcac aaaggttccg
    12121 gtcagtatcc ttcttttgac ctcctcgccg aacccgtaac ttctggtccg ggtgaactgc
    12181 gagaccaggt ccggagcttc ctccctgaaa ccgtatctca cgccgtcaaa ccttgcaaga
    12241 tttgaactcg cctcggcggg agcgatgatg tagtagcatg gcagtccgaa ggaagccgac
    12301 tcattgaggg agacctcgac catggtgcaa cccgaggata ccaacaccga agcggcctcc
    12361 tctattgatc ttttaaggga ggggtcgagg gggccatcga ggaactcccg gacgatccct
    12421 atccgcatcc ccttcactgc agggttggaa gcaccgtcac ggaaactggg gcgttctctc
    12481 cggctgcagg tagagtccct aaagtcctgg ggcgatatta catccatggc aagggcgata
    12541 tcacctacgc ttcttccgaa ggggcctatc tggtcgaggg aggaggcaaa ggccacaagc
    12601 ccccaccggc ttaccatccc gtaggtgggc ttgaggccgt ggactccgca gaaggcagcc
    12661 ggctgcctga tggagccgcc agtgtcacta cccagggcaa tgggcacgta gccggcagcc
    12721 acggaagcgg cgcttcctcc ggagcttccc cctggaaccc tggagatgtc cctggggttc
    12781 gacgcagggc cgaaacagga gtgctccgtg gaactgccca tggcgaattc gtccatgttc
    12841 gatttgccca caatcactgc cccggccctc tcgaaaagtt cgactacggc ggcatcatag
    12901 ggaggttccc agtcatccag tatcctgctg ccgcaggttg tcctgacacc ccgggtgcac
    12961 atgttgtctt tcacgacgac aggaacaccg gcaagaggtc ctggatcgca ccccgaggag
    13021 acctggccat caacatgggc ggccctctca agggcatact cctcgatcac ggttatcaga
    13081 gcattcagcc ggggttcaag actgcggatc ctgcgcagtg aggcagacac cacttctact
    13141 gcggagacct caccactggt cacggccgag gcgatactgg aagcatccat ctgttcatgc
    13201 ttcatcgggg ccctcctcgc tacctatcgc cggtacccgg aagaaccttc cctctcttag
    13261 cggtgcggaa tcgagggcct catccccgat atcccaacgg accggtccat ctcccctgag
    13321 ggggcatggt tcatcttcaa gggttgagaa gggatccact ccggtagtgt cgcacttctc
    13381 aaggatcctg aagtgcccga tgatgtcctg gaagaatctg accatagtct ccacattatc
    13441 tggtaccatc tctattcttg agagggttgc ggctcgttcc acgatcttcc tgtcgatctc
    13501 catgctatct ctcaacctcc ggtctttctc ccccggtagc cctttcgatc aggtcgagga
    13561 aggtcttctc atcgataacc ctgatcccca gagtgacggc cttcgccagt ttgctcccgg
    13621 gggcggaacc gctgaccacg aaagaggtct tccggctgac cgaggatggc gtcgatcctc
    13681 caagctcctt gaccctgttc tcagcttcgg aacgggtcat cccttccagt tccccggtaa
    13741 agacaaaact cagcccctta aggggaccca caccacctct ctgtggactc ccagaggagg
    13801 atacgttcag ccccgctgcc ttaagccttg caatggtttc ccggttccgg gggtccctga
    13861 agaaagcagc aaccgaagag gctatcctcg gcccgacacc ctcgatagaa gcgaggctgt
    13921 cttcgtcgca ggacccgagg tcctccaggc tgccaaaagc ctctgccaaa atctcggcca
    13981 ccctggaccc gacgtacctg atccccagag ctgctatcag agccgacagg ggtctttcct
    14041 ttgaagagct gatggagtcc aggacggaag atgcagcctt ctcaccaaga accctggatg
    14101 acttttcccc cgtgaggagt atcggggcca ggtcacccat cgaaagagaa tagatgtcag
    14161 ctatatcact aacgacccct tcttccacaa gcttctccag gagtttctcc ccaagccctc
    14221 cgatgtccat ccccgacctg gaggcaaagt gcctgagacc ctccaggatc tgggccgggc
    14281 aggacctgtt gacgcatctg agggccacct cccccggaag cgagatagcg tctgatccgc
    14341 aggcagggca acgggatggc atcctgaacg gaacagagct ttctttccgg gaagccaggt
    14401 cgggtcttag cacttcgggt attatctctc cagccttgcg cacccagacc atgtctccct
    14461 cacggatatc cttcctggcc acctcgtcgg cgttgtgaag gctggccctt tgaactaccg
    14521 tccctgaaag ggcgacgggc tcaaggatgg caatgggtgt cagtgtcccg gtccttccca
    14581 cggacacctc tatccttacc agcctggtca gcttttcctc ggggggatac ttgtacgcga
    14641 cggcccacct ggggaccttg gcggtggtcc cgagaccgga ccacgccccc aggtcgtcga
    14701 cctttatcac cactccgtct gtaacgtagg gaagcgagaa cctttcgttc ctccaacgtt
    14761 cgatgtagga aaaagcctcc tcggcccccc ggcagaggca ctccgtcccc tggaccggga
    14821 aacccatctt ccgaagccag gaaaggcttc tgaactggct ggaaaggccg tgatcgccgg
    14881 gggagaccac ctggtagacg aagaggctga gcctccttga agcagtcaca gagggatcca
    14941 gctgtctgag acttcctgcg gcggcgttgc gtggatttgc gaagaggggc agcccctgct
    15001 cctctcttga ggcgttcagg gccatgaagt cctcgctctt gataagaacc tcccctctga
    15061 cctccagtct tcccgggacc ttcccagcca gatcgcgagg gagatcggag atggtaagca
    15121 gattcctcgt gacgacctct ccggtccggc catcacccct ggtggcaccg aggatgaact
    15181 tcccttcgtt gtaatggagc gaaacggcaa gcccgtcgat cttgagctcg cagcacaggg
    15241 agggataggc tgcccctttt gaaaccttcc tcaggaagtc ctccacatct tccctgctga
    15301 aagcattttc caggctgagc atgggagagt cgtggacaac ctttacgaac tcgttcctgg
    15361 gcgctcctcc aaccgtcgct gtcggagagt ccggtggagg ctgctcaccg gttaaagcct
    15421 ccagctcctt cagctcacgg agcagaagat cataacggtc atcggagatc tctggagagt
    15481 caaggacgta gtagaggtag ttgtgacgtt ttatctcctc tctgagacgg ccgatccttt
    15541 cccttatttc ctgatctccc aaggcttctc cccccgtccc cgttccccta caccttcggg
    15601 cgcatggtcg ggaagaggat tacgtccctg atggaccggg aatcggtgag gaacatggca
    15661 agacggtcaa tacctatacc gagaccccct gtgggtggaa gaccgtactc aagggcgttg
    15721 atatagtcct cgtcgaagac gtgggcctcg tcgtcgccgg cctccttttt cttcaactgg
    15781 tccatgaagc gctccctctg gtcaagggga tcgttcaact cactgaaggc attggccacc
    15841 tcagagccat agatatagag ctcgaaacgg ttcgtgaaat ccggatcctc ggggtttttc
//
LOCUS       DAOPKQ010000008        15450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_4017_length_15450_cov_4.881065, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000008 DAOPKQ010000000
VERSION     DAOPKQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 15450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15450
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4017_length_15450_cov_4.881065"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..618)
                     /locus_tag="PK146_00620"
     CDS             complement(112..618)
                     /locus_tag="PK146_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPE90770.1"
                     /translation="MKERTPLPEQIAVDPVEKVRAFLEEAGYEGEIHFSEETIRTVDD
                     ASRSVGAPPEEILKTIVLLADGRPVIALMSGPNRIDLKKVKALLDARKVSMARPEWVL
                     EYSGFEVGGVPPVGFPSRPCAVIDRDLFRFEVVWAAAGTDHAFFPVNPRKLAGYTGGV
                     EADIKKEG"
     gene            complement(615..2612)
                     /locus_tag="PK146_00625"
     CDS             complement(615..2612)
                     /locus_tag="PK146_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPE90771.1"
                     /translation="MRKGLTLLLIITLLLPLLALTPRAEASEIEDLFWSRQWSTLESR
                     ILSPGSKVSPKERVIYANSLWLQGRWAPALEIFLEERDSLPEDLQPFTDMLILLGLER
                     TGSPKKARDVALSLWNKEDWVLAPYVAYALGRFYLNDGQAAEAGRWFRAMAERARDAS
                     LRRQALEALLKLPDPDPGFALALLEISPFHRGALSMIRQDPGPEDTSTAFPLGYAAFF
                     SGDFQGALAHLERVPLEDPRSERAAFFRARTLSNLGKGDEALQLWKELALKASRYSQT
                     SIESISRMAQEKGGKATEVLMEIAGTGTGDPAASALAELANLFARIGEEDKASRFEEI
                     LLEKHASSRFTAAALWEKGWSAWKRGDIRAADAAWTRALDAGNDRRQESRLLYWTAKA
                     NERLGDSRTATYLLERLRKSYPTDYYTFLAFPSGPGRVQNIVPDQLAGGGGKLAEWGF
                     VIYARMQLSSSDDPASRFTSARLALWLGDARSALLDSIPLVPLLARYDNIPLPLLETL
                     YPRAREREVMAASLRFGIDPVDIWAIMRRESAFDEEAVSSAGAMGLMQLMPPTARENA
                     MMLGEKEGDYFDPAKNILLGAYHFSRLMKMFDRIEFAVAAYNAGQGAVGRWLPPKRSV
                     DEWVEDIPYGETREFVRQVMANRYIYRITYPEMGQKGDQQE"
     gene            complement(2597..3097)
                     /locus_tag="PK146_00630"
     CDS             complement(2597..3097)
                     /locus_tag="PK146_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPE90772.1"
                     /translation="MSNLSARSEDYLKAIFKLQEEKKVVRVKDLAQCLGVRSPTVVGV
                     ISGLKESGLVDQEHYGYISLTPEGDTLARSLIQNEDLLRSFFEEVLSLDTEEAGLNAC
                     NIEHYITPTCRERLVSFIKFLEICGRGSPKWLDHFRHFVLTGEPPPCDRCGEILPGDG
                     KRCEKD"
     gene            3174..4052
                     /locus_tag="PK146_00635"
     CDS             3174..4052
                     /locus_tag="PK146_00635"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HPE90773.1"
                     /translation="MSLHAALGVIAVSATLWEVFIAPKPGLVDRFGPGSHRDMDFVTF
                     LLSASALAPFWQKQALEGLRGVPPEEALNVLRRTGIEMDRAMFEATSGVNTHKGLIFA
                     LSLLLYGAGRCSFLSEGLRPEKVAAAAAAAVSGCCERELGSLESEPPARPLTSGERIY
                     LEHGMTGIRGEAERGFPTVLEHGIPPFRRALARGVTRHDSALYALFSLMKHGEDTNIV
                     ARKGYPFWKADYLPLVENLLAHEIPYDTGALGSISEMDEFFSLHRISPGGAADLLTCT
                     LFLHDCDTLFPDVVNI"
     gene            4099..4455
                     /locus_tag="PK146_00640"
     CDS             4099..4455
                     /locus_tag="PK146_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPE90774.1"
                     /translation="MSLGLRIKTLRRSKGLTQKKLSEIVDVSRIYIQSLESNRRLPSM
                     KLLKRLAEALDVDVVDLVQDPYRDSTGRLMLEEVLNGREPVEIWYRSRKLSRGEVQLV
                     QKLIEAALSDWDREDG"
     gene            4448..4876
                     /locus_tag="PK146_00645"
     CDS             4448..4876
                     /locus_tag="PK146_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HPE90775.1"
                     /translation="MAETALGDTCTDGFEWPSPPERIPDWAFEEASHWKGLDDLDLLA
                     KAEEACGRSVEIVTLPLPISIWGLHVARGQRARIYLNSLLPEIWKRFAAFHEIYHLVH
                     HTRGEAFWSGTATPMTSFEYQADMFAWAAVFDEWTEGVSW"
     gene            4873..5814
                     /locus_tag="PK146_00650"
     CDS             4873..5814
                     /locus_tag="PK146_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE90776.1"
                     /translation="MRRIPFFLPMKDCPNRCVYCDQRTITGHGVQPAPGEIRRALQGA
                     VEPLETCFFGGSFTCQPMDRQEEYLEVAAQAPASSRIRISTHPLCVDPGILDFLSRFP
                     VRFLELGISSLEDKVLNLCNRGYTGATAVERVSLVTDSTWLVPGVQLMTGLPGQDPSS
                     SLEDIRVLAEIKGDRPMQLRIYPCLVLRGTPLEELFLSGSYTPPGIDESARWAGRMIR
                     KARESGFELLRVGLQETASLGEGVVAGPHHPALGELARSYALALSLVHRARSGPWSVP
                     LSYRSLLSGHGNWGFRELASLSEIAIDEVKRLVTWTR"
     gene            5896..7281
                     /locus_tag="PK146_00655"
     CDS             5896..7281
                     /locus_tag="PK146_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPE90777.1"
                     /translation="MAILKRNLDMGAGSVAGTLWQLALPSMFSMLFHTMFHLVDTVFV
                     SWLGESSLAAMSLSFPLVFVVFALVNGMAVGATTRMSNSLGKGKQGEARDYADAALVL
                     VLVLSTLVMPLLFRRSSNAFFSLLGGSGDVLVESHRYAFWMVLATPLMSFSLVADSIF
                     RSQGDTVTPMKSMILGNGINVLLDPLLIFTFGWGIAGASLATFIGRLASCLYLWSRLK
                     ERSAIIPAVTWRPDHPGRWISIGRIGLPVAISQGSMAVGGAMLNRILAGYGPAAIGAF
                     MLGNRVEMFVFLPVFGFNSALIPFIGYNIGKGDYPRVRGALRVVLLSSAVMIGVIGAM
                     VYMWPHLVLALFRPSPQVMGMAVSSIRASATAYLFAAADISFWGVFQGSGYPVYGMIA
                     QVMRTLAIRIPAAAILSRAFGLSGVWWCQPISAFVSFLLSTYFIFKVMDKIKVGIELS
                     SENGEDQKRKQ"
     gene            7635..8504
                     /locus_tag="PK146_00660"
     CDS             7635..8504
                     /locus_tag="PK146_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPE90778.1"
                     /translation="MEAGQLQELLREKMQGMPRKARRVVEYLLMNMREAAFRSIGDVA
                     DELGVSKAQLVRVSRMLGFDGYSQLKDALKSAVLGQVNPAAMLARVLDNRQDLPVTIH
                     RLEHANLDDTWTQIKPDDVSLFCSKIQEAETVYCAGWGISSLVAESLFIRLRTMGLRA
                     ILMTRSGMTLQEQARAVKRGDLVVVCELPSFVIEVTEAVEIAQRNGAGIVSITDSPAA
                     PICRFSDLSFFVSATSPMFGSSIIGPLFLVHILTSVLAVNMGDPARMALEEQAQFLHD
                     ERIFHPVFGLKYS"
     gene            complement(8511..9194)
                     /locus_tag="PK146_00665"
     CDS             complement(8511..9194)
                     /locus_tag="PK146_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPE90779.1"
                     /translation="MARLTFLGHAAFHLEGRALDALIDPFLTGNDMTHYSPRDFPDVN
                     YIFVTHGHGDHLGDTPEIAKKTGATVVATVEMCSELAKDKIKSHPLQIGGAFTFPFGR
                     VKMTPAWHGSGIQCEKGMLYGGVAGGFLIEVDGHKIYHAGDTGLTVEMGLLADDGVDV
                     ALLPIGGNYTMDIDDAVRAVRMIRPRIAVPMHFDTFPVIKASPEEFSSKAAGLAEIRV
                     LKVGESLEI"
     gene            complement(9227..9541)
                     /locus_tag="PK146_00670"
     CDS             complement(9227..9541)
                     /locus_tag="PK146_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90780.1"
                     /translation="MRIRGRGVRIRKKTMAWHFHLDEEGGSLKGELQVDGWEGSGEMN
                     QWFEKNHREEVEMVLKGMGRVRLTPRGIRIHESGHHNESIVKVEGFLLETLKEDEDPR
                     LI"
     gene            complement(9517..11373)
                     /gene="aspS"
                     /locus_tag="PK146_00675"
     CDS             complement(9517..11373)
                     /gene="aspS"
                     /locus_tag="PK146_00675"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPE90781.1"
                     /translation="MAEMRQEEGTPMKSQFFDRSWKRTSYCGYVPVPGEKGAEVLLNG
                     WLRRRRDLGGIIFIELWDHTGTVQVVFDPAMGSLYEKAKGLRNEFVLAVRGRVRKRPE
                     GTENPSMPTGDREVVVEDFIVLSPSRPLPFEISEADRVDENLRLRSRYLDLRRPFMQN
                     NLRLRSKAAEFTRSYLTGKGFVEIETPMLTKSTPEGARDYLVPARVNPGRFFALPQSP
                     QIFKQILMVGGIDRYYQVVKCFRDEDLRADRQPEFTQIDIEMSFITEEDIMFLMEGYM
                     KGLFQSVLGETLEDTFPVLPWHEAIDRFGSDKPDMRIESEILDLDNSFRECSFEGFRR
                     VLGEGGVVRGLMIPGGAAMSRKEISDLEARARELGASGLASFVIRDSALKGPLVKFLS
                     DGAQQDLVKRTGLTEKDALVVAAGPWRETCEILGHLRLETVTSSSQVSEGTWRFLWVT
                     DFPLLEWDEEGSRWSAVHHPFTSPNLEDLDLLEKDPGRVRSRAYDLVLNGTEIGGGSI
                     RIHDPSVQQRVFEVMDFTPERARERFGFLIDALASGTPPHGGIAIGFDRLVMFLCGVH
                     SIRDVIAFPKTQKAQCLMSGAPSDVDQAQLEELHISLDLAGEGGECGSGGGE"
     gene            complement(11361..12626)
                     /gene="hisS"
                     /locus_tag="PK146_00680"
     CDS             complement(11361..12626)
                     /gene="hisS"
                     /locus_tag="PK146_00680"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPE90782.1"
                     /translation="MSSIKAPRGVRDILPDESWKWEYVFAVTRKAASDFGYREVHLPV
                     FEHTELFSRGVGETTDVVEKEMYTFEDRAGRSLTLRPELSASMVRSYLENRMDRQHQP
                     AKLWSAGPMFRYERPQKGRYRQFWQIDFEAIGSQDPFVDVESVELSLEIFRRLGLSNL
                     EVVVNSVGCPVCRPVHKKALQNFLRPRLNDLCETCQARFDRNPLRILDCKNPSCRALT
                     DDATGTISCLCEECSEHFEMVKKGLEMIGASFRIDNRLVRGLDYYTKTAYEVLSGDLG
                     AQNAVCGGGRYDNLAESIGGPHTPGVGFATGVERIVLTMEQQGCSFGEAPNIDVFVIS
                     ADGSSRKEAVTALYDIRRAGISADIDYTGRSFKAQFKAAGSSGAVFACILGPEEVASG
                     QVTLKDLRTAEQKSVRRAELTDFLKTWLR"
     gene            complement(12816..13076)
                     /locus_tag="PK146_00685"
     CDS             complement(12816..13076)
                     /locus_tag="PK146_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HPE90783.1"
                     /translation="MEVLKVSANSQPKSVAGAIAAVVRESGAVEIQAVGAGAVNQTVK
                     SIAIARGYVSPNGIDLVCIPSFAKIDIDGEERTAMKFQLENR"
     gene            complement(13238..15079)
                     /locus_tag="PK146_00690"
     CDS             complement(13238..15079)
                     /locus_tag="PK146_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPE90784.1"
                     /translation="MNGYQGRVAMIELGERRYRDIFLDEGLVRKYIGGSGLGTYFLLK
                     FCTPGTKPLSPENPLIFTTGPFQGTGIPTSGRHQVVTRSPLTGIFLESDAGGSFGFHL
                     KRAGYDGLIITGRSERPVYLKVLDGQIGFEDGQDLWGLDTMETDARLKSVFGERIGVS
                     CIGPAGEKQIPLAGIFHDGTDARAAGRGGAGAVMGSKNLKAIVASGSSGTEVHDRKAL
                     TVLISEKNAILKEKGAGMTLYGTVGGMGMAEELGDLPIRNWKQGSWKEEVREITGQRM
                     AETILTGNYGCVACPVRCGRVVNIGGGNIGGPEYETMGMLGSNCLVNDLDAIARANEQ
                     CNRLGIDTISTGSVIAFAMECYEHGILTGEQAGMPLHWGSGEAVNYLVTKIGNGEGIG
                     GLLGRGTRAAAKELGRGAERFAIHSKGLEFPAHDPRCFKSLAAGYATSVRGACHLNGY
                     SYAWERSATFPEFGYPEKQDRTTDKGKGIMNARFQNLSSLVDSLKICKFILLMGVNLS
                     DISRWLKCVTGWEYSIKELEETGERIYNMKRIFNAGLGLDRSDDTLPQRILAEPRGSG
                     GSANTLPDLGMQLDEYYSYRGWSNEGIPLEEKLEGSGLREFMNWIPS"
     gene            complement(15101..15370)
                     /locus_tag="PK146_00695"
     CDS             complement(15101..15370)
                     /locus_tag="PK146_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90785.1"
                     /translation="MSRKGFTLKFYGPWQRAAGVEELFLPQESVTTVWCLIKKLEARF
                     GEEFSSHREGLVCLYDSVKGPRALDLNEDLLPGSTILFLGIVESG"
BASE COUNT         3119 a         4666 c         4109 g         3556 t
ORIGIN      
        1 tctgaaggtc gttaccttcc acaacctgga ggtcttcata cgcccactga ggtcgatccg
       61 cctcaaccgg gaattcgacg gctggttgga agcgatagta cccgagaagc gttagccctc
      121 cttcttaatg tccgcctcga caccacctgt gtagcctgcc agcttccggg gattgacagg
      181 gaagaaggcg tgatcggttc ctgccgccgc ccatactacc tcgaaccgga aaaggtccct
      241 gtcgatcacg gcacagggcc tggagggaaa accaaccggg ggcaccccgc ccacttcgaa
      301 cccggaatac tccaggaccc actccggcct tgccatggag accttcctgg catccaggag
      361 ggctttgacc tttttcaggt cgatcctgtt aggccccgac atcagtgcaa tgacaggtct
      421 tccgtcggcc aaaaggacaa tggtcttcag gatctcctcc gggggagctc ccacgcttct
      481 ggaggcatcg tcaacggtcc ttatcgtctc ctcgctgaaa tggatctccc cttcatagcc
      541 tgcttcttcg aggaaagccc tgaccttttc tacgggatcc acggcgatct gctccgggag
      601 tggcgtccgt tctttcattc ctgctgatct cccttctgcc ccatctccgg gtaggtgatc
      661 ctgtagatat atctgttcgc catgacctgt cgaacgaact cccttgtctc gccgtaaggt
      721 atatcctcga cccactcatc gacagacctc tttgggggga gccatcttcc aacagcaccc
      781 tggcccgcgt tgtaagctgc cacggcgaat tctatccggt caaacatctt catcagcctc
      841 gagaagtgat atgcccccaa caggatgttc tttgcgggat cgaagtaatc gccctccttc
      901 tccccgagca tcattgcgtt ctctctcgcc gtggggggca tcagttgcat cagccccatg
      961 gcaccagcgc tgctgacagc ctcttcgtcg aaggcgctct cacggcgcat gatagcccaa
     1021 atgtccaccg gatcaatgcc gaacctcaag gaggccgcca tcacctctct ctccctggcc
     1081 cgtggataga gagtctctag aagggggagg ggtatgttat cataccgggc cagcagcggg
     1141 accaggggga tggaatccaa aagcgcgctc ctggcgtcac ccagccaaag tgcaagcctg
     1201 gcagaggtga acctggatgc ggggtcatcc gatgaagaga gctgcattct cgcatagatg
     1261 acgaaccccc attcggccag tttgccgcct ccgccggcca gttggtcggg gacgatattc
     1321 tgcactcttc cagggccgga gggaaaggca aggaacgtgt agtaatccgt cgggtaactc
     1381 ttccggagcc tctcaaggag atatgttgcg gtccttgaat cacccagcct ctcgttcgcc
     1441 tttgcagtcc agtacaaaag acgggattcc tgccttcggt cgtttcctgc atcaagggcc
     1501 ctggtccagg ctgcatcggc agccctgata tcccccctct tccaggcaga ccaccccttc
     1561 tcccacaggg ctgccgcggt gaagcggctc gaggcatgct tctccaggag tatctcctcg
     1621 aatcgcgaag ccttgtcctc ttcccctatc ctggcgaaga ggtttgctag ttcggccagt
     1681 gccgaggcgg caggatcgcc agttcctgta ccggcgatct ccatcagcac ttcagtagct
     1741 tttcctccct tttcctgggc catcctggaa atggactcga tcgaggtctg cgagtacctt
     1801 gaagccttca gggccagttc cttccagagc tgcagggcct cgtcgccctt tccgaggttc
     1861 gaaagggttc ttgccctgaa gaaggcagcc ctctctgatc ttggatcttc gagggggact
     1921 cgttcgaggt gggccagggc tccctggaag tcgccggaga aaaaagcagc ataccccagg
     1981 gggaaagcgg tacttgtgtc ttccggtccc ggatcctgtc tgatcattga gagggctccc
     2041 ctgtgaaagg gggagatctc gagaagagcc aacgcaaacc ccggatcggg gtcgggaagc
     2101 ttgagaaggg cttccagggc ctgtctgcgc agtgatgcat ccctggccct ctctgccatc
     2161 gccctgaacc atctccctgc ctctgccgcc tggccatcat tcaggtagaa ccttcccagg
     2221 gcatatgcta cataaggtgc aagtacccag tcctccttgt tccagaggga aagggccaca
     2281 tccctcgctt tctttggaga tcctgtcctt tcgagaccca gaagaatgag catgtccgtg
     2341 aagggttgaa gatcttccgg aagggagtct ctctcctcga ggaagatctc cagggcaggg
     2401 gcccatctcc cctggagcca gagagagttg gcatagatca ctcgttcttt tggagatacc
     2461 ttgcttccag gtgaaaggat cctgctttca agggtagacc actgccttga ccagaaaaga
     2521 tcctctattt ccgatgcctc tgcccttggg gtcaaggcaa gaaggggcag caaaagggta
     2581 ataataagta gaagggtcag tccttttcgc atctctttcc gtcccctggc agtatttcgc
     2641 cgcatctgtc gcaggggggt ggctctcctg tcaggacaaa gtgcctgaaa tggtcgagcc
     2701 acttcgggga tcccctcccg catatctcca ggaattttat gaaggatacc agtcgctccc
     2761 tgcaagtcgg ggtaatataa tgctctatgt tgcaggcgtt aagtcctgcc tcctcggtgt
     2821 ccaggctgag aacctcctcg aagaaggaac gaaggagatc ctcgttctgg ataagggatc
     2881 ttgcaagggt gtccccctca ggggtcaggg agatgtaccc gtagtgctcc tggtcgacca
     2941 gcccgctttc cttgaggccg gaaatgactc ccacgacggt gggcgatctc actccgaggc
     3001 actgggccag gtccttgacc cggaccactt tcttctcctc ctggagcttg aatatggcct
     3061 taagataatc ttcgcttctt gcactgaggt tagacattgt tagcctcgcc tatttttgtt
     3121 aatatccagt tcagtatccg cttttttcaa aacccagtca agggagagag aaaatgtccc
     3181 ttcacgccgc tcttggtgtc attgccgtct ctgcgaccct gtgggaagtt ttcatagccc
     3241 caaaacctgg tcttgtcgac cgttttggtc caggttcgca tcgggacatg gattttgtca
     3301 ctttcctcct gagcgcttca gccctggcac ccttctggca aaaacaggcc ctggagggtc
     3361 tgagaggagt tcccccggag gaggcactca acgtcctgcg cagaaccggg atagagatgg
     3421 acagggccat gttcgaggcc acttctggtg tcaacactca caagggcctg atcttcgccc
     3481 tgtcgctcct cctctacgga gcgggccgct gttcctttct cagtgaaggt ctccggccag
     3541 aaaaggtggc cgccgcagcc gccgcggctg tcagcgggtg ctgcgaaagg gaactgggat
     3601 cgcttgagag cgaaccccct gcgagaccac tgaccagcgg tgagagaata tatctggaac
     3661 acggtatgac aggtatcagg ggtgaggccg agagaggttt ccctaccgtc ctggaacacg
     3721 gcatccctcc cttcaggagg gccctggcca ggggagtaac caggcatgac agcgctcttt
     3781 acgcactctt cagcctcatg aagcacgggg aggacaccaa catcgtagcc aggaaaggtt
     3841 atcctttctg gaaagccgac tacctccccc tggtggagaa ccttctggcc catgagatcc
     3901 cctacgacac cggagccctg ggatcgataa gtgagatgga cgagttcttc agcctacaca
     3961 ggatcagccc gggaggggcg gctgaccttc tgacatgcac cctcttcctt cacgactgtg
     4021 atactctctt ccccgacgtg gtaaacattt aatactacag gtataatata tgtaacataa
     4081 tgaacggagg tatgggccat gagcctggga ctaaggatca agacccttcg cagatcgaag
     4141 ggactaaccc agaagaaact ttccgaaata gtggatgtga gccgcatata catccagtcc
     4201 ctggagagca acaggagact gccttcaatg aagcttctga aacgcctggc cgaggccctc
     4261 gacgtggacg tggtcgacct ggtacaggat ccctaccggg acagcacggg aaggctcatg
     4321 ctggaagagg tgcttaacgg ccgggaaccg gtggagatct ggtaccggag caggaagctt
     4381 tccaggggtg aggtgcagct tgtccagaag ctcattgagg cggctctctc cgactgggac
     4441 agggaagatg gctgaaactg ccctgggaga cacatgcact gacgggttcg agtggccatc
     4501 cccacctgag aggatcccgg attgggcctt cgaagaggcc tctcactgga agggacttga
     4561 cgatctcgac cttctggcta aagcggaaga ggcatgcgga aggtctgtcg agatcgtaac
     4621 tctgccgttg ccgatatcta tctggggtct gcatgtggcc agaggtcaaa gggcacggat
     4681 ctatcttaac agcctcctcc ctgagatctg gaagcgcttc gccgccttcc acgagatata
     4741 ccatctggtt catcatacga gaggcgaagc cttctggtcg gggacggcca cccctatgac
     4801 cagtttcgaa tatcaggcgg acatgttcgc ctgggccgct gtgttcgacg agtggaccga
     4861 aggggtctcc tggtgaggcg cattcccttc tttcttccca tgaaggactg ccctaacagg
     4921 tgcgtatact gcgaccagag gaccatcacc ggccatggcg ttcaacccgc tccgggtgag
     4981 atcagaaggg cccttcaggg ggccgtcgaa cccctggaga cgtgcttttt cggcggcagc
     5041 ttcacatgtc agcccatgga ccgtcaggag gaatacctgg aagttgcggc acaggctccc
     5101 gcttccagcc ggataaggat ctccacccac cctctttgcg tcgatccggg gatcctcgac
     5161 tttctttcac ggttcccggt gagatttctg gagctgggaa tctcctccct cgaggacaag
     5221 gtgctgaacc tgtgcaacag gggttacacc ggagccacgg ccgtggagag ggtctccctg
     5281 gtaaccgaca gtacctggct cgttcctgga gtgcagctca tgacaggcct tccgggtcag
     5341 gacccgtcat catcgctgga ggacataagg gttctcgctg aaataaaggg agacagaccc
     5401 atgcagttga ggatataccc ttgcctggta ctcaggggca ccccgctgga ggagctcttc
     5461 ctctccggaa gttacacacc tcccgggata gatgaaagtg cccgctgggc cggaagaatg
     5521 atccgcaaag cccgggagtc aggtttcgaa ctcctccgtg tcggcctcca ggagacggcc
     5581 tcccttggcg aaggcgtcgt ggccggacct caccaccctg ccctgggtga gttggcacgc
     5641 tcttacgccc tggccctttc gcttgttcat cgggcgcggt cgggtccatg gtctgtccct
     5701 ttaagttacc ggtcccttct ctcgggacat gggaattggg gtttccggga acttgcgtcc
     5761 ctttcagaga tcgccatcga tgaggtaaaa cgcctggtca catggacccg atgaaacttc
     5821 cacgaacacg aaaacatttg cccccggcgc cttttttaca tacaatgctc tgagtcttcc
     5881 actgcggagg gatcaatggc catcctcaag aggaacctgg acatgggcgc cgggagcgtc
     5941 gccggaacac tctggcagct tgccttgcct tcgatgttct ccatgctctt ccataccatg
     6001 ttccacctgg tcgatacggt cttcgtatca tggctggggg agtcctctct ggccgccatg
     6061 tcactttcct ttccccttgt cttcgttgtc ttcgccctgg tgaacggcat ggccgtgggg
     6121 gccaccacca ggatgagcaa ctccctgggc aaggggaagc aaggtgaagc ccgggactat
     6181 gccgacgcag ctcttgtcct tgtcctggtc ctttcgacac tggtgatgcc cctgcttttc
     6241 cgtcgatctt caaacgcctt tttctccctc cttggcggtt ccggagacgt cctcgtcgaa
     6301 agccaccgtt atgctttctg gatggtcctt gcaaccccgc tgatgtcctt ctccctggtt
     6361 gccgactcca tcttcaggag ccagggggat acggtgaccc ccatgaagag catgatcctc
     6421 ggcaacggga tcaacgtcct gcttgacccc cttctgatat tcaccttcgg atgggggata
     6481 gccggtgcgt cgctagccac cttcatcggc cggctggcct catgcctgta cctgtggtcg
     6541 aggctcaaag aacggagtgc gatcattccg gccgtaacct ggagacccga ccaccctggc
     6601 agatggatca gcataggcag gatcggtctt ccagtggcaa tatcccaggg cagcatggcc
     6661 gtcggaggtg caatgctgaa caggatcctc gcaggctatg gccctgcagc cataggtgcc
     6721 ttcatgctgg gcaacagggt agagatgttc gtatttctcc ccgtattcgg cttcaactct
     6781 gctcttattc ccttcatcgg ctacaacatc gggaaaggcg attatcccag ggtccgcgga
     6841 gcgttgagag tggtccttct ctcctcggcg gtcatgatcg gcgttatcgg ggccatggtc
     6901 tacatgtggc cccatcttgt gcttgccctc ttcagacctt cgcctcaggt tatgggaatg
     6961 gccgtctcat ccatcagggc ttcggcgacg gcctatcttt ttgcagcagc ggacatctct
     7021 ttctggggtg tcttccaggg aagcggttac ccagtttacg gaatgatcgc ccaggtaatg
     7081 aggaccctgg cgatccgaat tccggcggca gctattctct ccagggcctt cggtctttcc
     7141 ggggtttggt ggtgccagcc gatctctgcc ttcgtctcct tcctcctgtc gacctacttc
     7201 atattcaagg tgatggacaa gatcaaagtt gggatagaac tgtcatcgga gaatggagag
     7261 gatcaaaagc gtaaacagtg aacggtaggg acaagcgaaa gcccagatcc ttcacttcgc
     7321 tcacaaaata ttatggtcat atctgcgaca ataccctacg aagacagttc gccatcacgg
     7381 gcagagcgtc tgcggtgcaa aggatttggc atttacccct tctgttcacc gttgaccgtt
     7441 gaccgttcac cgttcacgcc tttgaatttg gttctcccgc ctcaagggtt ttctgccttt
     7501 cctgctgcct gtcgcctgac gccttgcttc aggtttcctg cctccgttga cttccaccag
     7561 gacccaaagt atgctaaagt caactttttg cctgaatggt tcatttcaaa agggctttcg
     7621 ggaggtcatt ccaggtggaa gctggacagt tgcaggagct tctaagggaa aagatgcagg
     7681 gcatgccaag aaaggccagg cgggtcgtcg aatatcttct tatgaacatg agggaagcgg
     7741 ccttccggtc cataggagat gtggccgacg aactgggagt atccaaggcc cagctggtaa
     7801 gggtctcaag gatgctgggt tttgacggct actctcagct gaaggacgca ctcaaatccg
     7861 ctgtgctggg acaggtcaac ccggcggcga tgcttgcgag ggttttggac aaccgccagg
     7921 acctcccggt gaccatccac aggctggagc acgcgaacct ggacgataca tggacccaga
     7981 tcaaacccga tgatgtctca ctcttctgct caaagatcca ggaagcggaa acagtctact
     8041 gtgccgggtg gggcatctca tccctggtgg ctgaatcgct tttcatcagg ctaaggacca
     8101 tggggttgcg ggccatcctc atgaccaggt ccggaatgac cctccaggaa caggccagag
     8161 cagtcaaacg gggcgacctg gtggtggtct gcgaactccc cagtttcgtg atcgaggtca
     8221 ctgaagcggt ggagatagcc caaaggaacg gagccgggat cgtctccatt accgacagcc
     8281 ccgcggcccc gatctgcagg ttctcggacc tctccttctt tgtaagcgcc accagcccga
     8341 tgttcgggag cagtattatc ggccccctct tcctggtcca tatcctgacc tcggtccttg
     8401 ccgtaaatat gggtgacccg gcaaggatgg cactggagga acaggcccag ttccttcacg
     8461 acgaaaggat cttccacccc gttttcgggt taaagtactc ctgatatggt tcagatctcc
     8521 aggctctccc ccaccttcag gacccttatc tctgcgagcc cggcggcctt ggaggaaaac
     8581 tcctcggggg aggcctttat caccgggaaa gtatcgaagt gcatgggtac agcgatgcgg
     8641 ggtctgatca ttctgacggc ccgaactgca tcgtcgatat ccatggtgta gttcccaccg
     8701 atagggagaa gtgcgacatc gacaccatcg tcggccagca gccccatctc gacggtgagc
     8761 cccgtgtcgc ctgcatggta gatcttatgt ccgtcgacct cgatcaggaa cccgccagcc
     8821 acacccccgt atagcatgcc cttctcacac tggatcccag aaccgtgcca ggcaggcgtc
     8881 atcttcaccc tcccgaaggg gaaggtaaag gctcctccta tctgcagggg atggctttta
     8941 atcttgtcct tcgccagttc gctgcacatc tcgacagtcg cgaccaccgt cgccccggtc
     9001 ttctttgcta tctcgggggt atctccgaga tggtctccgt gtccgtgggt tacgaaaata
     9061 tagttgacat ccgggaaatc ccggggagag taatgtgtca tatcattccc cgtgaggaag
     9121 ggatctatca aggcatcgag ggctcgccct tccagatgaa aagccgcatg tccaagaaat
     9181 gtcaacctgg ccattttcct gcctccccat tgtctgattt cctccattat atcagccggg
     9241 gatcctcatc ctccttcagt gtctcgagaa ggaaaccctc aacctttacg atgctctcgt
     9301 tatgatgacc tgattcatgg atacggatcc ccctgggggt cagccggact ctccccatgc
     9361 ctttgagcac catctcgacc tcttcccggt ggttcttttc gaaccactgg ttcatctccc
     9421 ccgatccttc ccaaccgtcc acctggagtt cccctttcag ggaaccgcct tcctcatcca
     9481 gatgaaagtg ccaggccatg gtcttctttc ttatccttac tccccgcccc ctgatccgca
     9541 ctctccaccc tccccggcaa gatccagcga gatgtgaagt tcctccagct gcgcctggtc
     9601 aacgtctgaa ggtgctcccg acatcaggca ctgggccttc tgggtcttcg ggaaagcgat
     9661 cacatcccgt atcgaatgaa ccccgcacag gaacatgacc aacctgtcga aaccgatggc
     9721 aatgccaccg tgaggtggag ttcccgaggc aagggcgtcg atcaggaaac cgaacctctc
     9781 cctggcccgt tcaggggtga agtccattac ctcgaacacc ctctgctgga ccgaagggtc
     9841 gtggatcctt atggagcctc caccgatctc ggtaccgttc aacaccaggt cataagccct
     9901 ggatcttacc ctgcccggat ccttctcgag gagatcgagg tcctcgagat tgggagaggt
     9961 aaaggggtgg tggacggctg accagcgtga tccctcctcg tcccactcaa ggagtgggaa
    10021 atccgtaacc cagaggaatc tccaggtccc ctccgatacc tgggaggaac tggtcactgt
    10081 ctcaagacgc agatgaccca ggatctcgca ggtctccctc cagggtcctg ctgcaacaac
    10141 cagcgcgtct ttctcggtaa ggcctgtcct cttcaccagg tcttgctgag caccatcgga
    10201 gaggaacttg accagaggcc ccttgagtgc gctatcccgg atcacgaatg aggcaaggcc
    10261 agatgccccc agttcccttg ccctggcttc gagatcggag atctcctttc gcgacatggc
    10321 cgcgcctccg ggtatcataa gcccccttac gactccgccc tctcccagga ccctccgaaa
    10381 gccctcgaag gagcactctc tgaaactgtt gtccaggtcg aggatctccg attctattct
    10441 catgtccggt ttgtcgctgc cgaaacggtc gatggcctca tgccagggga gaaccgggaa
    10501 ggtatcttca agggtctcgc caaggacgct ctggaaaagc cctttcatgt acccttccat
    10561 gaggaacatt atgtcctcct cggtaatgaa gctcatctct atgtctatct gggtgaactc
    10621 gggctgtcgg tcggcccgga gatcctcatc cctgaagcac ttgactacct gatagtagcg
    10681 gtcgatgccc cctaccatga ggatctgttt gaatatctgc ggtgactggg gaagggcaaa
    10741 gaaacggccg gggttcaccc ttgccggtac aagataatcc ctggccccct ccggggtgga
    10801 tttggtcagc atgggcgtct cgatctcgac gaaccccttg cctgtgaggt aggacctggt
    10861 gaactccgca gccttgctcc tgagcctcag gttgttctgc atgaagggac gcctgagatc
    10921 aaggtatctg gatctcaggc ggaggttctc gtcaaccctg tcggcttcgg atatctcgaa
    10981 gggaagaggc cgtgatggag aaaggacaat gaaatcttca acaacgacct ccctgtcacc
    11041 ggtcggcatg gaagggttct ccgtaccttc aggtctcttc ctgaccctcc ccctgactgc
    11101 caggacgaac tcattcctca ggccctttgc tttttcatag aggctcccca tggctgggtc
    11161 aaagaccacc tggaccgtgc cggtgtggtc ccacagttcg atgaagatga ttccgcccag
    11221 gtcccttctc cggcggagcc atccgttgag gagcacttcg gctcccttct ctcccggcac
    11281 aggcacgtaa ccgcaatagg aagtcctctt ccagcttctg tcgaagaact ggcttttcat
    11341 gggggtccct tcttcctgtc tcatctcagc catgtcttca ggaagtccgt cagttcggct
    11401 cttctgactg acttctgttc agcggttcgc aggtccttca gggtgacttg tcccgacgct
    11461 acctcctcag gcccgagaat gcaggcaaaa acagcaccgc tactaccggc agccttgaac
    11521 tgtgccttga agctcctgcc cgtgtagtcg atatcggcgg atatcccggc ccttcgtatg
    11581 tcgtagagag ccgtaacggc ttccttcctg gaggatccat cggcagagat cacgaaaaca
    11641 tcaatgttcg gggcttcccc gaaagaacac ccctgctgtt ccatggtgag gactatcctc
    11701 tccactcccg tcgcaaatcc cactccagga gtatggggac cccctatgga ctcggccagg
    11761 ttatcgtagc gccctccgcc gcagactgcg ttctgggcac cgagatctcc tgaaaggacc
    11821 tcgtaggcag tcttggtgta gtagtccagt ccacgaacaa gcctgttgtc tattctgaaa
    11881 gaggccccga tcatctcgag cccctttttt accatctcaa agtgttctga acactcttcg
    11941 cagaggcagg atatggtccc cgtagcgtca tcggtcaggg cccgacagga cgggttcttg
    12001 cagtccagga tcctcagggg attacggtcg aaccttgcct ggcaggtctc gcagagatcg
    12061 ttcagtctcg gtctcaggaa attctggagg gcctttttat ggactggcct gcagacggga
    12121 cagcccactg aattgaccac cacctccaga ttggaaagcc cgagtctcct gaatatctcc
    12181 agggaaagct ccactgactc cacgtccaca aagggatcct ggcttccgat ggcctcgaag
    12241 tctatctgcc agaactggcg gtatcgcccc ttctggggtc gctcgtagcg gaacatcggc
    12301 cctgcgctcc agagttttgc cggctgatgc tgcctgtcca tcctgttctc caggtaggat
    12361 cgtaccatcg acgccgagag ctccgggcgg agagtaaggc ttctcccggc acggtcctcg
    12421 aaagtgtaca tctccttctc gaccacatcg gtggtctctc cgacacccct tgagaaaagc
    12481 tcggtatgtt cgaacacggg aaggtggacc tccctgtagc cgaaatcact tgcagccttt
    12541 ctcgttacgg caaagacata ctcccacttc cacgattcgt cgggaagtat atcccggact
    12601 ccccgcgggg ccttgatgct ggacatggaa aaccctcctt gctgcagttt gcctgatcgg
    12661 aatcgttaga ttgtagcact agttgcagtg gtcatgaaac tggtgatcaa aaagggttgt
    12721 tactccgaag gagagaggcc agacgcaagt ggtcaaaaaa cccgcgggcc cttacggccc
    12781 gcgggttcta ggatctggat cggactgaca acggttcagc ggttttcgag ctggaacttc
    12841 atggccgttc tttcctcgcc gtcgatgtct atcttggcga aggaagggat acagacgaga
    12901 tcgatcccgt tgggcgagac ataccccctt gcgatagcta tagacttgac cgtctggtta
    12961 acagcacccg ctcctaccgc ctggatctcg accgcaccgc tctctcttac cacagccgcg
    13021 atcgcgccgg cgacagattt tggctgagag tttgcagaga ccttgagtac ttccatggaa
    13081 tatcgccctc ccctggattc cacgatactg ctccgctgtc accttcgatc cactctttac
    13141 cgcattacgc atctgtaacg ataagtatac ctttattttc ccttgtcaac aataccccta
    13201 cccaaaaaag atttcaataa aatgaaatcc gtgcctctca ggaggggatc cagttcatga
    13261 actctctcaa gcccgatcct tcaagctttt cctcaagagg gatcccctcg ttgctccatc
    13321 ccctgtagct gtaatactca tcgagctgca tccctaggtc tggaagcgta ttggcagaac
    13381 ctccgctacc cctgggttct gcaaggatcc tttgagggag tgtgtcgtcg ctcctgtcaa
    13441 gtcccaagcc ggcattaaag atccgtttca tattgtagat gcgttctccc gtctcctcca
    13501 gctccttgat cgaatactcc caacccgtca cacacttcag ccaccgcgag atatcagaaa
    13561 ggtttacccc cataagaagg atgaatttgc atatcttgag cgaatcgacg aggctggaaa
    13621 gattctggaa cctggcgttc attatcccct tgcctttgtc ggtggtcctg tcctgcttct
    13681 cggggtaccc gaactccggg aaagtggcgg acctctccca ggcataggaa tatccattca
    13741 gatgacaggc ccctctcacc gacgttgcgt agccggcagc aaggcttttg aaacacctcg
    13801 ggtcatgggc agggaattcc agccctttgg agtggatggc gaacctttcc gcccctcttc
    13861 ccagctcttt tgcggccgct cgcgttcccc tcccgaggag gcctccgatc ccttcaccat
    13921 tgcctatctt ggtcaccagg tagttcacgg cctcaccact tccccagtga aggggcatcc
    13981 ctgcctgttc accggtgagg atcccgtgtt cgtaacactc catcgcgaag gcgataaccg
    14041 aaccggtgga tatggtgtct atcccgagac ggttgcactg ttcgttggcc ctggctatgg
    14101 catccaggtc gttgaccagg cagttgcttc ccagcatgcc catggtctcg tattccggac
    14161 caccgatatt tcccccgccg atattgacga ccctcccgca tctgacagga catgcaacac
    14221 atccgtaatt gcctgtgagt atcgtctcgg ccatcctctg gcctgttatc tccctgacct
    14281 cttccttcca ggaaccctgt ttccagttcc tgattggaag gtcccccagc tcctccgcca
    14341 tccccattcc gccgacggtt ccgtagaggg tcattcccgc tcccttttct ttgaggatgg
    14401 cgttcttttc tgatatcagg accgtgaggg cctttctgtc atgaacttcc gtcccggagc
    14461 tcccgctggc aacaatagcc ttgaggttct tgctgcccat tacggctcct gcccctcctc
    14521 ttcctgcggc tctggcgtca gtaccgtcgt ggaagatccc tgcaaggggt atctgttttt
    14581 cacctgcggg gcctatgcag gagactccga tcctctcacc gaagaccgat ttgagcctcg
    14641 catcggtctc catggtgtcc agtccccaaa ggtcctggcc atcctcaaag cctatctggc
    14701 cgtccagcac cttcagataa accggtcttt cagaccttcc ggtaatgata aggccgtcgt
    14761 agcctgccct cttgagatgg aacccgaagc tgcctccagc atcggattcc aggaatatcc
    14821 ctgtaagagg tgacctggtt accacctggt gcctaccgga agttggtatg cctgtcccct
    14881 ggaaggggcc tgtagtgaag atcaaagggt tctccggcga tagaggtttg gtcccggggg
    14941 tgcagaactt taacaggaag taggtcccca gccccgatcc ccctatgtac tttctgacaa
    15001 gaccctcgtc gaggaagata tccctgtacc ttctctcccc caactcgatc attgcaactc
    15061 ttccctggta cccattcatt cctttccctc cctgccttgt ttaaccgctc tccacgatcc
    15121 ccaggaagag gatggtggag cccggaagaa gatcttcgtt cagatcaagg gccctgggac
    15181 cctttacact gtcataaaga cagaccaacc cctccctgtg ggaggagaac tcctctccga
    15241 accgggcttc cagcttcttg ataagacacc aaacggtcgt gacactctcc tggggcagga
    15301 agagctcttc cacaccggcc gcccgctgcc atgggccata gaatttcagc gtaaaaccct
    15361 tgcgtgacat cagtccgaag gttcctctct ggagtgaaga atgtagtcaa ggagatcggc
    15421 cctgctgatg accccggaaa agatgccatc
//
LOCUS       DAOPKQ010000009        15068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_4146_length_15068_cov_5.298275, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000009 DAOPKQ010000000
VERSION     DAOPKQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 15068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15068
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4146_length_15068_cov_5.298275"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PK146_00700"
     CDS             <1..495
                     /locus_tag="PK146_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90786.1"
                     /translation="LSSLRQARIRYPRIDWLVLSSGDDPDVVRGCICLGVFDYLIKPF
                     STERLERALSAYYQYYQGLTQRTNPWRQKELDLITGLKGRFFSGLDEYPKGIQVKLLE
                     RLRACMEERDKAMSASTAGSYVGISRSTARRYLEYLVERRQATVEYDISSVGRPVKLY
                     RLIL"
     gene            complement(502..1524)
                     /locus_tag="PK146_00705"
     CDS             complement(502..1524)
                     /locus_tag="PK146_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPE90787.1"
                     /translation="MDLRSIPVEEALGHALAHDLTKIDTEKGIKGARFRRGHILQPED
                     LPVLREMGRRNLSIIALEPDEVHEDEAALSLAKALAGEGFEISGPEEGRCRLVSTGRG
                     IVNFDPEAVRRINRDREWSFACTHINSVVGPGSVVAAFRILPLCLKRDSLERAVEEAS
                     PFSLIPFIHRRVGLVSTGQEILEGLVKDSFREKLERKLWELGGSFSGHEICGDRPADI
                     VNAIDELLGMGSDIVVCTGGMSIDADDRTPQAIETASDEVLFRGVPMIPGSNLMLAMK
                     GLSWIIGAPACVAHDERTALDRLLIALFAGLGGEINVSLWGEGGLCRRCRTCIFPECE
                     FARIPS"
     gene            complement(1528..2391)
                     /locus_tag="PK146_00710"
     CDS             complement(1528..2391)
                     /locus_tag="PK146_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HPE90788.1"
                     /translation="MDRALLKSVHEELESGGAGALCTVVGSEGSTPRDLGASMWVRLD
                     GSIAGTVGGGSLEYAVIVKALELLRSASGPVLHKAVLRTGDSGVGAVCGGETVILIEP
                     LGQEAEVVIFGAGHVGKAVARAAYSAGFRVVVWDEREEFANPESVPWGKTLACPLEKA
                     FDEGVRLHGSSYVVVVTRGHSLDGEVVRILEGKPMAYIGMIGSRKKISYVRESLIKQG
                     VSKDFLDRIFQPVGLPVRAETPEEIAVSILAEIIAVRRGADIASLRSSYSREAGPLFP
                     YLPIVIPGNEE"
     gene            complement(2360..2791)
                     /locus_tag="PK146_00715"
     CDS             complement(2360..2791)
                     /locus_tag="PK146_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPE90789.1"
                     /translation="MGWGNVFQGDVRFCNEGFPSFHWASGDLALKDVSGPGPAIAFVR
                     PDRVRFLDPRMPVDSELSRNTFSGKVEEVLLDGGVLRLHVRTPRGHWQIEQRETGSHS
                     EVFRQGDEVHFAIPPGAIELIFLEEMKGEENLGSSIVEVRA"
     gene            complement(2996..3493)
                     /locus_tag="PK146_00720"
                     /pseudo
     CDS             complement(2996..3493)
                     /locus_tag="PK146_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3477..4121)
                     /gene="modB"
                     /locus_tag="PK146_00725"
     CDS             complement(3477..4121)
                     /gene="modB"
                     /locus_tag="PK146_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HPE90790.1"
                     /translation="MERALIISLKVLAIDIPLLALLGGGIGWLLARRDFRGRELASLL
                     VQLPIILPPSVLGLYLLFALGQINFFREAGFLFAFPAAVLGALLPSLPIMIQASRAAF
                     AGVDRELEEAALTLGRRPWEVFLRVTLPLARRTLLVGLALSSARALGDFGVTLMVAGN
                     IPGRTQTLPLYIYGQVESLNFARAHMAALLLVGVGIASLCLVHRLESGAHERLA"
     gene            complement(4133..4882)
                     /gene="modA"
                     /locus_tag="PK146_00730"
     CDS             complement(4133..4882)
                     /gene="modA"
                     /locus_tag="PK146_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE90791.1"
                     /translation="MKRIIPEHFRVWAGLFMLLIFCPVTPGSAHAEVFAAAASLQPVM
                     EKISQEFEREKGIRMDAVFAASGQLAKQIEMGAPYDLFLSADEKWARYLEKKGKLEKV
                     RPVAECPLVLWWESAEDPSLDLVKDKRIRVAIADPEVAPYGALAKEYLEKIGEYENLN
                     NEERLIIAGDVLKAGLAAKSGGAGAAYIPLSIAKKLEGSWTNVPMPPQRLFGGIVKDR
                     DALAALVFYDYLLSPPAAELFREAGFEPVQD"
     gene            complement(4879..5319)
                     /locus_tag="PK146_00735"
     CDS             complement(4879..5319)
                     /locus_tag="PK146_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPE90792.1"
                     /translation="MDAREILQNTSIRLTPVREAILGVLLNERCPMSYREVAARLPGG
                     QDRVTLYRTLSLFLKEGIVHRVMDMKGTWRFCAHDPGHPGCPGNHPHFACRVCGRMEC
                     LMEHSLPHLEVGPETVVEGKHMVLYGICRGCAVKKTSKKEAGSS"
     gene            complement(5409..5840)
                     /locus_tag="PK146_00740"
     CDS             complement(5409..5840)
                     /locus_tag="PK146_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE90793.1"
                     /translation="MEKVYTGKIEDLELFDASSLAPEVTKRVVFGPGRFWDDYTMRHF
                     SLPAGTIIPDHVHDWPHYMIGLSGTGFVIVEGKPLEIPPMGWAHVPGGIVHGYENPGP
                     EPFVFLCIVPTEGDPHMKLKRFRMERSKSKVSKGIEETPTS"
     gene            complement(5891..8710)
                     /locus_tag="PK146_00745"
     CDS             complement(5891..8710)
                     /locus_tag="PK146_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE90794.1"
                     /translation="MTRKYLVKTLVINGITHRVVADPEEKLVNVIRKQLHLTGTKLGC
                     GQGQCGACNVILDGKVTRSCITPWSKVPENADILTIEGIGTPDHLHAIQWAFIANGAI
                     QCGFCTPGFIVAAKGLLDGNLNPTREEIRDWFQAHWTACRCTGYKQITDAVMDAAAVL
                     RGEKQMKDFAKSLKPGDSVWGTRYPRPSAVFKATGTFDFGDDAGLKLPTETLHAVPVC
                     PETSHAKVLSIDFSEAEKMPGVFKVVTARDIKANKGTNRIRGQAGSGTASTDGWERRI
                     LVEEGDKIRQWGEVVAIVCADSEEQARAAAAAVKVDLEELPAVMDVKAAMDPGAPKVY
                     EEIPDIDGIPNCFNKRPVIKGDDPAPMIDRAPYVVEAEFWSSRQPHMTIETDCGFGYW
                     DEEGRLTLQTKSICVYRHAMMIARGLGVPPSKIRVIQNNMGATFGYKVAPTNEPLLGA
                     CVIATGRPVYMRLNMKEHIVRTPKRSPFLMKVRVGADEKGKLVGLEHHWYVDHGPYSE
                     SSQDLTNKGGQFICAPYRLDNIRGAGYTIFTNQKWCGAFRAYGGPQTYFAGEQVIDML
                     AEKVGMDPLDFREINLIRPGDTLPSGQKPEVYPLQAMLTRIRPLYEEAKKRAELNSTD
                     KVKRAVGISIGIYNANDDGADEAASNIELTKDGVILYNTWEDHGQGADMGCVGTAHEA
                     LKPLKLHPDQIKLVCNDMAKAPNSGAAAASRCQVMVGNAIVDSCRKLLDAMRKPDGTY
                     RTYEEMFAEGIPTFYEGYWQATVRNVDGEAITCTGMDDVTGQGYPFANHMFGIFMAEV
                     AVDIKTGKTSVEKFTLVSDVGKINNLDVVEGQHFGGIAQGIGLALTEDFGDPAKYTNL
                     VTCGFPYIKDIPDDIQLFHMETPREFGPFGASGTGEMPLSAPHPAILNGIARACGARI
                     FQIPALPEKVLEGMKESGCSGKCGH"
     regulatory      complement(8785..8902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            9230..9700
                     /gene="nuoE"
                     /locus_tag="PK146_00750"
     CDS             9230..9700
                     /gene="nuoE"
                     /locus_tag="PK146_00750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPE90795.1"
                     /translation="MSPKKPETEERTKQIVAPWKGTHGGTIPVLQEVQKEFGYLPEDA
                     LMVISREMKIPPSELFGVATFYAQFHLNPRGRHIVRVCRGTACHVRGSLAILDKIKKV
                     LQIEENGTTEDLRYTLEPVACLGACGLAPVMMVDEETHGRLTPDKVMEILGSYE"
     gene            9724..10092
                     /locus_tag="PK146_00755"
     CDS             9724..10092
                     /locus_tag="PK146_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPE90796.1"
                     /translation="MKVKSLEDLRKIKENAKDLTSARSGGETRVIVGMGTCGIAAGAR
                     EVMTAILEELEKRGLRHVAVETTGCIGMCQREPLVDIIRPGEPRITYGDVSPGDVPGI
                     IEEHLVNGRVVEDKVVGQTD"
     gene            10107..11900
                     /gene="nuoF"
                     /locus_tag="PK146_00760"
     CDS             10107..11900
                     /gene="nuoF"
                     /locus_tag="PK146_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPE90797.1"
                     /translation="MAFYRAHVLVCKGTGCNASGSSSVFDALGEEIRKRGLDQEVALV
                     ETGCHGMCEMGPIVVIYPEGSLYCRVSVDDVPEIVEEHLYKGRLVQRLLYTTPDRKVK
                     VPRYRDIPFYSKQHRIALRNCGYINPDNIEEYIARDGYAALAKALTSMKPEEVLSEVK
                     TSGLRGRGGGGFPTGLKWEFCRKAQGDKKYVICNADEGDPGAFMDRSILEGDPHAIIE
                     GMTLGAYAMGADEGYIYCRAEYPLAIKRLVGAITHAEEFGLIGDNVMDTDFSFHLHIK
                     EGAGAFVCGEETALLASIEGERGMPRPRPPFPANKGLWGKPSNINNVETWANVPNIIR
                     NGGSWYASIGTEKSKGTKVFALTGKVNNTGLVEVPMGTTIRDIVFDMGGGILKDRKFK
                     AVQIGGPSGGCLTEEHLDLPVDYESLTAAGAIMGSGGLVVMDEDTCMVDVAKFFLEFT
                     QRESCGKCVPCREGTKQMLLILEKITSGKGRMEDLKILEDLAIMVKEMSLCGLGQTAP
                     NPVLTTLRYFRDEYETHIRDKKCPAKACSALISYLIDPEKCKKCGLCARNCPVKCISG
                     DRNTPYEIDQEKCIKCGTCLEVCPFQAVFKE"
     gene            11920..12270
                     /locus_tag="PK146_00765"
     CDS             11920..12270
                     /locus_tag="PK146_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPE90798.1"
                     /translation="MKDITLTIDGKACKGAEGNTILEVARRNDVYIPTLCFLEGLTPI
                     GSCRMCVVEVEKNPKMLTACTTPAQDGMVVHTQTEKLREYRRQMLELIFAGRNHFCMY
                     CSQSGDCELQDLAI"
     gene            12277..13974
                     /locus_tag="PK146_00770"
     CDS             12277..13974
                     /locus_tag="PK146_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPE90799.1"
                     /translation="MDSVRYPFLYAAFDNDATHRDIQVDHNRCILCLRCIRVCAEKVG
                     THTLDLQKRGWNATIVADLGRRLGESSTCTTCGACAQVCPTGTITLREFAYRGRRKDC
                     DDAVESVCPLCSVGCRIKAYVRTGSIVRVEGTSVTEPDGGQLCKKGRWTLPRSAERER
                     ISVPMIREGSHYREASWEEALELVAENFGKAHQADSSGALVSSLCTDEELALFVSLFK
                     DGLGMERLDTFDGDVLRGFVKGFEPFREQGIRPFTAAHNILNSDCIVNLSADPDEEAP
                     VIASYMRVGTLKNGATLLNLSCRTSPFADITDIDAVCPTPGQSAALVEGLAELVAGLK
                     ASGGKALEEGKISVGELAGKAGIPEETVEGILEKLAGSQRPVFVLGGCLSRDPVAVTS
                     AVNLAIASRAFFEDGMGVVPLVVSGNSLGTINTVLAREPWLGREKLDFLYVYSTGLVP
                     EDEESLSAISGTKFVVVHCPFWAQPLVNLADVLLPAPAWFERSGHYCTIEGERRRLNV
                     VVPPKGEIRGLSSILKDLSAGLGVEPGKPKESPCGNLFASEKPPADARMVSLEEVRN"
     gene            13974..14513
                     /locus_tag="PK146_00775"
     CDS             13974..14513
                     /locus_tag="PK146_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HPE90800.1"
                     /translation="MSKVKVATVWLEACAGCHMSFLDIDERIVELLEKVEIIYSPIVD
                     SKEIPKATVGVIDGALGNDEELHLARKMRKQCDIIVAWGDCAVFGGINCMRNFSDRQC
                     ALKEAYLDSPSTVNPEGIIPHDDIPQLLPQAIPVDHEVKVDVYVPGCPPDADTILWVF
                     QELLEGRIPKVPSEKIRYD"
     gene            14527..>15068
                     /locus_tag="PK146_00780"
     CDS             14527..>15068
                     /locus_tag="PK146_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPE90801.1"
                     /translation="MATVKKIEINPITRIEGHGKITVHLDENGNVNEARFHVTQFRGF
                     EHFCKGRDFREMPVITPRICGICPVSHHLASAKACDALLGVELTLTAHKLRELMHMGQ
                     FVQSHALSFFHLSSPDLLFGFDADPAIRNVAGVIEKYPRLAVKGVLLRKFGQEIIQVL
                     GGKKVHPWMISCPNFLSRTP"
BASE COUNT         3300 a         4429 c         4125 g         3214 t
ORIGIN      
        1 ctttcctctc tcagacaagc aagaataagg tacccccgca tagattggct tgtcctctcc
       61 agcggtgacg atccggatgt cgtccgcgga tgtatatgcc tgggtgtctt cgattacctg
      121 ataaaaccct tctcgacgga acggctggag cgggctcttt cggcgtacta tcaatactac
      181 cagggtctga cgcaaaggac caatccatgg cgtcagaaag aacttgatct gatcaccggt
      241 ctgaagggaa ggttcttcag cggcctcgat gaatacccca aaggaatcca ggtgaaactt
      301 ctggaacgtc ttcgtgcctg tatggaagag cgggacaagg ccatgtcagc ttcaacggct
      361 ggatcctatg tggggatatc ccgttcgaca gcaagacgct atctggagta cctggttgaa
      421 aggaggcagg ctactgtcga atacgacata agcagcgtcg gccgtcccgt caaactttac
      481 aggctcatcc tttgaagccc ctcaggaagg tatcctggca aactcgcatt cggggaagat
      541 gcaggttctg catcttctgc aaaggccccc ttctccccag agggagacgt ttatctcccc
      601 tccaagtccg gcaaaaagtg ctatcagcag tcgatccagt gcagtccgct catcgtgggc
      661 cacacaggcc ggggcgccta ttatccacga aagacctttc atggccagca tcaggttcga
      721 tccagggatc atcggaacac cccgaaagag aacctcatca gaggcagtct cgatagcctg
      781 gggtgtgcga tcatcggcat cgatgctcat tcccccggta cagaccacaa tgtccgaccc
      841 catcccgagc agttcatcaa tagcgtttac aatatcagcc ggtctgtcgc cgcagatctc
      901 atgaccggag aaacttcccc caagttccca aagcttcctc tccagttttt cacggaagga
      961 atctttaacc aggccctcca ggatctcctg acctgttgaa acaaggccga ccctcctgtg
     1021 gatgaagggg atcagggaaa agggcgaggc ctcctcgaca gctcgctcaa gagaatccct
     1081 tttcaggcaa agtggaagga tcctgaaggc ggcaaccacg cttccgggcc ccacaacaga
     1141 gtttatgtga gtgcaggcaa aggaccattc gcggtctctg ttgatccttc tcacagcctc
     1201 agggtcaaaa ttgacaatcc cccttccagt ggagaccagt ctgcatctcc cctcttcggg
     1261 tcctgagatc tcaaaccctt cgccggcaag ggctttcgca aggctcaagg ctgcctcgtc
     1321 ctcgtgcacc tcgtcgggtt ctagtgcaat aatcgaaaga tttctccgcc ccatctccct
     1381 gagaacggga agatcctcgg gctgcagaat atgccctctc ctgaatctcg cccccttgat
     1441 ccccttttcg gtatcgatct ttgtaaggtc atgggcaagg gcatgtccaa gagcctcttc
     1501 aacaggtatc gatcttagat ccacggccta ctcctcattt ccgggaatta cgatcggaag
     1561 ataagggaaa agaggccctg cttccctcga ataggaactt ctgagactgg caatatccgc
     1621 tccccttctg accgctatta tctcggcaag aattgacacg gctatctctt caggagtctc
     1681 ggccctgaca ggaagcccca ccggctggaa gatcctgtcg agaaaatctt tggagacccc
     1741 ctgcttgatc aggctctccc tgacatagga tatcttcttc cttgacccga tcattccaat
     1801 ataggccatc ggcttccctt caagtatgcg gaccacttcg ccatccagcg agtgtcccct
     1861 ggtcactacg acgacgtatg aagatccatg tagtctgacg ccctcgtcaa aagctttttc
     1921 cagaggacag gctagcgttt tcccccaggg gactgactcc ggatttgcga actcctctct
     1981 ttcgtcccag acgacgacac ggaacccggc actatatgca gccctggcaa ccgctttgcc
     2041 gacatgcccg gccccgaaga taaccacctc cgcctcctgg ccgagaggct ctatcaggat
     2101 cacggtctct cctccacata ctgcgccgac gccactgtca ccggttcgaa ggactgcctt
     2161 gtgaagaaca ggtcccgaag ctgatcgaag gagttccaat gccttcacta tgacagcgta
     2221 ttcaagtgag cctccaccca ccgttcctgc gatggatccg tcaagccgga cccacatgga
     2281 ggccccaaga tcacgaggtg tggatccttc agaacctact acagtacaaa gcgctccggc
     2341 tcccccggat tccagttctt catgcacgga cttcaacaat gctcgatcca agattctctt
     2401 ctcccttcat ctcctcgaga aagatcaatt ctatcgcccc gggtggaatg gcaaaatgca
     2461 cctcgtcacc ctggcggaaa acctctgaat gggaccccgt ttccctctgt tctatctgcc
     2521 agtgtccgcg aggggtccgg acatgcaacc tgaggacccc tccgtcaagc agcacctcct
     2581 cgacctttcc ggaaaaggtg ttcctggaaa gttcgctatc cacgggcata cgggggtcaa
     2641 gaaacctgac ccggtcaggc ctcacgaaag cgatagccgg acctgggcct gatacgtctt
     2701 taagtgccag gtcaccggaa gcccaatgga atgaggggaa cccttcattg cagaaacgga
     2761 cgtcgccctg aaacacattt ccccagccca tcatcttcca gagatcgacc ctgcctctgc
     2821 ctctgatcag atcgctgata cggtttgaac ctgtcaattt acctttctcg ataaagaaaa
     2881 ccttgtctcc aagagcacag atatcctcca cctggtgggt cacatagaga atggggaccc
     2941 ctgtctcggt gtggagcttt cgcaactccc ttctaaggtt ccgcctcaga ggtccgtcaa
     3001 gggcactgaa gggttcgtcc agcataagca tttcaggttc gttggcaaga gctctcgcca
     3061 gggctacgcg ttgtctctga ccccccgata gctgggatgg gtaacgattc tccagggatt
     3121 ccagacggac ccgggcaagc cactcccggg ctatcgattt cgctcttctt tttccggtgc
     3181 tttcacaacc gaagagaaca ttttcaaggg cggtcaagtg ggggaaaagc gccaggttct
     3241 ggaacagaag ccccattttc cgccggtggg ggggctcgca tatgcctgcg tccgaatcca
     3301 gcaggacacg tccggacaat gcaattagtc cgaaccggac gtttctcaaa ccggcaagac
     3361 accgcaaggt gatactcttg cccgacccgc tgggaccgaa aagcactccg atctccctgc
     3421 caagccttaa agatacatca aggtggaaat ctccaatcaa ttcattgata tgagcatcaa
     3481 gcaaggcgtt catgagctcc ggactccagc ctgtgaacca ggcagaggct ggcaatacca
     3541 actcccacca gcagaagggc cgccatgtgc gcgcgggcga aattcagcga ttccacctgt
     3601 ccgtagatat acaggggcaa agtctgggtt ctaccaggaa tgttccctgc caccatcagc
     3661 gtgacgccga aatcgcccag ggctctggct gaggagaggg ctaatcctac caacagggtt
     3721 cttctggcca ggggaagggt cacacgcagg aaaacttccc atggtcttct cccaagggtc
     3781 agggccgcct cctccagttc cctgtccaca ccggcaaagg ctgcccgtga agcctgtatc
     3841 attatcggca gcgacggaag gagcgccccc aggacagctg caggaaaggc gaacaggaat
     3901 cctgcctccc ggaaaaaatt gatctgtccc agggcaaaaa ggaggtagag cccgagaacc
     3961 gaggggggca ggattattgg aagctggaca agaagtgacg ccagctcccg tcccctgaag
     4021 tccctccttg ccaggagcca cccgataccc ccacccagaa gggcaagcag gggtatatca
     4081 atagccagta ctttcagact gatgatcagg gcccgttcca ttattgctcc ggtcagtcct
     4141 gaacaggttc gaaccctgct tcccggaaca gttctgcagc cggcggtgaa aggaggtaat
     4201 catagaaaac cagtgccgcc aatgcgtccc ggtctttaac gatccccccg aaaagacgct
     4261 ggggcggcat cgggacgttc gtccaggaac cttcaagttt tttcgcgatg ctgaggggaa
     4321 tataggctgc cccggcacct ccgctctttg cggcaagacc cgccttgagg acatcgccgg
     4381 ctattatcag gcgttcctca ttgttcagat tctcatactc tccgatcttt tcaagatact
     4441 ccttcgccag ggctccatag ggcgcgacct cggggtcggc gatcgccacc cttatcctct
     4501 tgtcctttac caggtccagg gaagggtcct ccgccgattc ccaccagagg accagtggac
     4561 actctgccac cggtctgacc ttctccagtt ttcccttctt ctcgagatac cgtgcccatt
     4621 tttcgtcggc gctcaggaac aggtcgtagg gtgcccccat ctctatctgc tttgccagtt
     4681 gtcctgatgc cgcaaaaacc gcatccatgc ggatgccctt ctccctttcg aactcctggg
     4741 agatcttctc cataacaggc tgaaggctcg ccgcagccgc aaagacctcc gcatgggcgc
     4801 tgcccggggt caccggacag aatatcaaca acataaataa ccctgcccac accctgaaat
     4861 gttccggtat gatcctcttc atgaagatcc agcctccttc ttgctggttt tctttacagc
     4921 acagcccctg cagatcccgt ataagaccat atgctttcct tctacaacgg tttccggccc
     4981 cacttccagg tgcggcaagg agtgttccat caggcactcc atccgtccgc agacccggca
     5041 ggcaaaatga gggtggttcc ccggacaccc ggggtgaccg ggatcgtggg cacagaaacg
     5101 ccatgtcccc ttcatgtcca tgaccctgtg gacgatcccc tctttcagga acagcgaaag
     5161 cgtcctgtac agggtcacac gatcctgccc cccggggagt cgagctgcca cttccctgta
     5221 tgacataggg catctctcgt tcagcaggac tccaagaatt gcttcccgta ccggagtcag
     5281 ccgaatggac gtgttttgca gaatttcccg tgcgtccata tttttctcct ttctgcaaca
     5341 ctgttgcatt tatctttcat agaaaagccg cccgtttttc cacacggacg gctttcctcc
     5401 gttaatatct agcttgtcgg agtttcttct attcccttcg aaaccttact tttggagcgc
     5461 tccatgcgaa aacgtttcag tttcatatgc ggatcaccct cggtgggaac gatgcacagg
     5521 aaaacgaagg gttccggtcc ggggttctcg tagccgtgga caatcccgcc cggaacatga
     5581 gcccaaccca tcggagggat ctccagaggt ttaccttcga cgatcacgaa accggtaccg
     5641 ctcagaccta tcatgtaatg cggccagtcg tgaacgtgat caggaataat ggtgccggca
     5701 ggcagggaaa aatgcctcat cgtatagtcg tcccagaatc tgccggggcc gaagacaacc
     5761 cgcttggtca cttccggagc cagggaggac gcatcgaaga gttccagatc ttcgatcttc
     5821 ccggtataga ctttctccat tattcagcac ctcctcctgt ctgagctcac aactgcaagg
     5881 gacaggacct ctaatgaccg cattttccag aacagccgct ctccttcata ccctcaagga
     5941 ccttttctgg gagagccggt atctggaaga tcctcgcccc gcatgccctg gctatgccgt
     6001 tcaatattgc cgggtggggc gccgaaaggg gcatctctcc cgtccccgat gcaccgaagg
     6061 gaccgaactc cctgggtgtc tccatgtgga agagctggat gtcgtccggg atatccttga
     6121 tgtaggggaa tccgcaggtg accaggttcg tatattttgc cggatctccg aaatcctcag
     6181 tcagggcaag gcctattccc tgggctattc ccccgaagtg ctgcccttcc acaacatcga
     6241 ggttgttgat cttgcccacg tcgctgacaa gggtgaactt ttccaccgat gtctttccag
     6301 tcttgatgtc cacggctacc tccgccatga agatgccgaa catgtggttt gcaaaggggt
     6361 acccctggcc tgtaacgtca tccatcccgg tgcaggtgat ggcctcaccg tcaacgttac
     6421 ggaccgtggc ctgccagtaa ccctcgtaga atgtcgggat cccttcggca aacatttcct
     6481 cgtaggtcct gtacgttccg tccggtttcc tcatggcatc caggagtttg cggcagctgt
     6541 cgactatcgc gttgccaacc ataacctggc atctgctcgc cgcggcggcc ccgctgttgg
     6601 gggctttcgc catatcgttg cataccagtt ttatctggtc gggatgcagt ttcagaggct
     6661 tcagggcctc gtgtgccgtt cccacgcaac ccatatccgc accctgtcca tgatcctccc
     6721 aggtgttgta gagtatcact ccgtcttttg ttagctcgat attgcttgcg gcttcatcgg
     6781 ccccgtcgtc gttggcgtta tagatcccga tagagatacc gacggctctt ttgactttgt
     6841 cagtggagtt cagttcggcc cgctttttgg cctcctcgta tagggggcga atacgtgtga
     6901 gcattgcctg caaggggtac acttccggtt tctggccgct gggaagtgtg tccccggggc
     6961 ggatcaggtt gatctcgcgg aagtccagag ggtccatccc caccttttcc gcaagcatgt
     7021 cgatgacctg ttcgcctgca aagtatgtct ggggtccgcc gtaggcccgg aaggcgccgc
     7081 accatttctg gttggtgaaa atggtgtagc cggctcccct gatgttgtcc aggcgatagg
     7141 gggcgcagat gaactggcca cccttgttcg tgagatcctg gctcgactcc gagtagggcc
     7201 catggtctac ataccagtga tgctccagac ctacaagttt tcccttttca tcggcaccga
     7261 ccctgacctt catgaggaag ggcgaccttt tgggggtcct gacgatgtgc tccttcatgt
     7321 tgagcctcat gtagaccggt cggccggtgg ctattacgca ggccccgagg aggggttcgt
     7381 tggtgggtgc caccttgtag ccgaaagtcg cccccatgtt gttctgtata acccttatct
     7441 tcgaaggagg aacacccagc cccctggcta tcatcattgc gtgcctgtat acgcagatgc
     7501 tcttcgtctg cagggtaaga cgcccctctt cgtcccagta accgaagcca cagtcagtct
     7561 cgatggtcat gtgaggctgc cgcgaactcc agaactcggc ctcgaccacg taaggggccc
     7621 tgtcgatcat gggtgccggg tcatcaccct tgatcacggg gcgtttgttg aagcagttcg
     7681 gtatcccgtc gatatcgggg atctcctcgt aaaccttggg ggctcccgga tccatcgctg
     7741 ccttgacgtc cattactgcg ggcagttcct caagatccac cttgacagcg gccgctgccg
     7801 ccctggcctg ctcttccgag tccgcgcaga ctatggccac gacctcaccc cactggcgga
     7861 tcttgtcgcc ctcctccacc aggatccttc tctcccatcc atcggtgctc gcggtgcccg
     7921 aacctgcctg tccgcggatg cggttggtcc ccttgttggc cttgatgtcc ctggcagtta
     7981 cgaccttgaa cactcccggc atcttctcgg cttcagaaaa gtctatcgac agcaccttcg
     8041 catggctagt ctcgggacat acaggaactg cgtgaagggt ctccgtcggc agcttcagcc
     8101 ctgcgtcgtc gccgaagtcg aaggtgccgg tggccttgaa gacggcgctt gggcggggat
     8161 accgggtacc ccacacggag tctccaggtt taagggattt cgcgaagtcc ttcatttgct
     8221 tctctcccct gagaacagcc gccgcatcca tgaccgcatc ggttatctgc ttgtacccgg
     8281 tgcatcggca ggctgtccag tgggcctgga accagtcacg gatctcttcg cgggtcggat
     8341 tgaggtttcc gtccagcaac cccttggcgg cgacaatgaa cccgggggtg cagaaaccgc
     8401 attgaatggc tccgttggcg ataaaggccc actggatagc gtggagatga tcaggggttc
     8461 ctattccttc tatcgtcagg atgtccgcat tctcgggaac tttgctccag ggtgttatgc
     8521 aggatcgggt taccttaccg tccaggatga cgttgcaggc tccgcattgt ccctgtccgc
     8581 accccagttt tgtgcctgtc agatgaagct gtttccttat cacgttgacc agtttctctt
     8641 cagggtcggc taccaccctg tgggtgatcc cattgatcac caaggtttta acaaggtatt
     8701 ttctggtcat gggttttccc tccatttttt ataaaatgag gaccattcag ggaagacatc
     8761 tgaagactgc gatctgtgcg atcgagcgag aatcggacac cccctttccg atcatgggag
     8821 gcgtccctgt ttccagggac gcctatcggt ctttgaccat ggctggcgct ttaggcactc
     8881 aggctcggag atccctgcaa aagcaattat tggttacaat atctaatgta tcagttcgac
     8941 tatattgcgc tctgacccgg taatcaattt ctgcttgaaa ttgaactttt agttcgatat
     9001 tgttcacgaa aaacccggga gaaataagga tttttgaccc tttcccggtt gcgcccttcc
     9061 ctttcataag tttcaattat tactggttgt ttttcagaaa cgccttacgg agacgtcggg
     9121 gaccatgatc taacgcgtga ggcaactttc accgcctgga acctcagccg gtggtgaccg
     9181 aggacagtaa gttgacaagg tattcttcag accagggagg aggatagggg tgagtccgaa
     9241 aaaacctgaa acggaagaaa gaacaaagca gatagtggcc ccgtggaaag gaacgcacgg
     9301 aggcacgata ccagtcctgc aggaggtcca gaaggagttc ggctatcttc ccgaagacgc
     9361 attgatggtg atctcccggg agatgaagat ccccccttca gagctcttcg gggtggccac
     9421 cttctatgcc cagttccatc tcaaccccag gggacggcac attgtccgtg tttgcagggg
     9481 tacggcatgc catgtcagag gcagcctggc catcctcgac aagatcaaga aggtgctcca
     9541 gatcgaggag aacggcacca ccgaagatct caggtacacc cttgagcccg tcgcttgcct
     9601 cggggcatgc gggctggcac cggtgatgat ggtcgacgaa gagacccacg gacgcctcac
     9661 gcccgacaag gtgatggaga tactcggttc ctacgaatag gcacaaactg gaggtgatca
     9721 ccggtgaaag tcaagagcct ggaagatcta aggaagatca aggaaaacgc caaggacctc
     9781 acgtcggccc gatccggggg cgagaccagg gtgatcgttg gtatgggcac ctgcgggatc
     9841 gcggcagggg ccagggaggt aatgacggcc atcctggagg aactggaaaa acgtggtctc
     9901 aggcatgtgg ccgtcgagac cacgggctgt atcggcatgt gccagagaga gccccttgtg
     9961 gacattatca ggcccggaga acctcgcata acctatgggg acgtctcacc tggcgatgtg
    10021 cccgggataa tagaggaaca ccttgtcaac gggagagtgg tcgaggacaa ggttgtcgga
    10081 caaactgact agggggcttg aagggaatgg ctttctatcg tgcccacgta ctggtctgca
    10141 aagggaccgg ctgtaacgcc agcggatcct cctctgtttt tgacgctctc ggggaggaga
    10201 taaggaaaag aggcctggac caggaagtag ccctggtcga gacggggtgt cacgggatgt
    10261 gcgagatggg ccccatagtg gtcatctacc cggagggttc tctctactgc agggtgtccg
    10321 tggacgatgt ccctgagatc gtagaggaac acctctacaa ggggcgcctg gtccagcgtc
    10381 ttctttacac cacccccgac aggaaggtca aggtcccccg ctacagggac atccccttct
    10441 acagcaaaca gcatcgtatc gcgctgcgaa actgcggcta catcaatcct gacaacatcg
    10501 aagagtacat tgccagggac ggttacgcag ctctcgccaa ggccctgacc tccatgaaac
    10561 cggaagaggt gctctccgaa gtcaagacat ccggactcag gggaagaggc ggcggcggtt
    10621 tccccacggg attgaaatgg gagttctgcc gaaaggcaca gggtgacaag aaatacgtaa
    10681 tctgcaacgc cgacgagggc gacccgggtg ccttcatgga caggtccatc cttgagggcg
    10741 acccccatgc aatcatcgag ggaatgacgc tgggcgctta cgccatgggg gctgacgagg
    10801 gctacatcta ctgccgtgcc gagtaccccc tggccatcaa gcgtcttgtc ggtgctatca
    10861 cccatgcaga ggagttcggc ctcatcggcg acaatgtcat ggacacggat ttctccttcc
    10921 atctgcacat aaaggaaggt gcaggggcct ttgtctgtgg cgaggagacg gcactcctgg
    10981 catccatcga gggcgaaaga gggatgccac gtcccagacc tcccttcccg gccaacaagg
    11041 gcctttgggg taaaccttcc aacatcaaca acgttgagac ctgggccaat gttccgaaca
    11101 taatccgcaa cgggggctcc tggtatgcct ccatcgggac ggaaaagtcg aaggggacaa
    11161 aggtcttcgc ccttaccggc aaggtcaaca acaccggcct ggtggaagtg cccatgggaa
    11221 cgaccatcag ggatatcgtc tttgatatgg gaggcgggat cctgaaggac agaaagttca
    11281 aggcggtcca gataggcgga ccttcgggag ggtgtcttac ggaagagcac ctggaccttc
    11341 cggtggatta cgagtccctg accgccgcgg gagccatcat gggttcgggc ggcctggtcg
    11401 tcatggacga agacacctgc atggtggatg tggccaagtt cttcctggag ttcacccaga
    11461 gggaatcctg cggaaaatgc gtcccttgtc gggagggcac caagcagatg ctgctgatcc
    11521 ttgaaaagat cacctcagga aagggcagga tggaggacct caaaatactg gaggacctgg
    11581 cgataatggt caaggagatg tccctttgcg gcctcggcca gaccgcgccc aaccctgtcc
    11641 ttactacact gaggtatttc cgggacgagt acgagaccca catcagggac aagaagtgtc
    11701 ccgcaaaggc ctgcagcgct ctcatctcct acctcatcga ccccgagaag tgcaaaaagt
    11761 gcggtctatg cgcaaggaat tgccctgtta aatgcatctc cggcgacagg aataccccct
    11821 atgagatcga ccaggaaaag tgcataaagt gcggaacgtg tcttgaggtc tgccccttcc
    11881 aggcagtctt caaagaataa gaggcggggg gatataagga tgaaggacat aacccttacc
    11941 atagacggta aagcatgcaa gggcgcagag gggaacacca tactcgaggt tgcccgcagg
    12001 aacgacgtgt acatacctac tctctgcttc ctggagggcc tgacccccat aggatcctgc
    12061 cgcatgtgcg ttgtcgaggt ggagaaaaac cccaagatgc ttacagcctg caccacccct
    12121 gcccaggacg gcatggtcgt gcacacacag acggagaagc tcagggaata ccggagacag
    12181 atgcttgaac tgatcttcgc cggtcggaac catttctgca tgtactgctc ccagagcggc
    12241 gactgcgaac tgcaggacct ggccatctaa cacggtatgg acagcgtccg ctaccccttt
    12301 ctctacgccg ctttcgataa cgatgccacc caccgggata tacaggttga ccacaaccgg
    12361 tgcatactct gcctgcgctg catcagggtt tgcgccgaga aggtggggac ccacacgctg
    12421 gaccttcaga aaaggggctg gaacgccacc atcgtcgccg acctgggacg aaggctcggg
    12481 gagagcagca cctgcaccac ttgtggagcc tgcgcgcagg tatgccccac cggtacgatc
    12541 actctcaggg agttcgccta cagggggcgc aggaaggact gcgatgacgc ggtcgagagc
    12601 gtatgccccc tctgttccgt cggatgccga ataaaggcct acgtgcggac cgggagcatt
    12661 gtcagggtcg aagggacctc cgttactgaa cccgacggcg gacagctctg caaaaagggg
    12721 cggtggaccc ttccaagatc tgccgagcga gagaggatct ccgtacccat gataagagag
    12781 ggctcccact acagggaagc cagttgggaa gaggcccttg aactggtggc ggagaacttc
    12841 gggaaggctc accaggccga cagcagtggg gcccttgtct ccagcctctg caccgacgag
    12901 gaactggccc ttttcgtatc cctgttcaag gacggcctgg gcatggagcg cctggacacc
    12961 tttgacggag acgttctaag gggcttcgtc aagggtttcg aacctttccg cgaacagggt
    13021 atacgccctt tcactgccgc tcataacatc ctgaacagcg actgcatagt caacctgtcg
    13081 gccgacccgg atgaagaagc ccctgtcata gccagctaca tgagggtggg caccctcaaa
    13141 aacggcgcaa ccctcttgaa cctgtcctgc aggacaagcc cctttgctga cataaccgac
    13201 atcgacgctg tatgcccgac acccggacag agcgcggccc tcgtcgaggg tctggcagag
    13261 ttggtcgccg gactgaaggc atcggggggc aaggcacttg aggaaggcaa gatatctgtc
    13321 ggagaactgg ccggaaaggc gggcatccct gaagagaccg ttgaagggat cctcgaaaag
    13381 cttgcgggct cccaaagacc cgtcttcgta cttggaggct gcctgtccag ggatccagtg
    13441 gcggtgacat cggcggtcaa ccttgccatt gcatccaggg ccttcttcga agacggcatg
    13501 ggagtggtcc cgctggtggt aagcggcaac agcctgggca ccattaatac cgttctcgcc
    13561 agggaaccct ggctcggaag ggaaaagctc gatttccttt acgtttactc caccggcctc
    13621 gtcccggagg acgaggagag tctttccgcc atatcgggga caaaattcgt ggtcgtgcac
    13681 tgccccttct gggcccagcc gctggtcaac ctcgccgatg tcctgctccc ggccccggcc
    13741 tggttcgagc gcagcggcca ctactgcacc atcgagggcg agaggcggcg gctgaacgtg
    13801 gtcgtgccgc ccaaggggga gatcaggggt ctctcttcaa tcctgaaaga cctctcggca
    13861 ggtctggggg tcgaaccggg aaaaccgaaa gaatctccat gcgggaacct gttcgccagt
    13921 gaaaagcctc cggcggacgc ccggatggtt tcgctcgagg aggtgcggaa ctgatgtcga
    13981 aggtcaaggt cgccacagtt tggctcgagg cctgcgctgg ttgtcatatg tcgttccttg
    14041 atatcgacga gcgcatagta gagctgcttg aaaaggtcga aataatatac tcacccatcg
    14101 tcgacagcaa ggagataccc aaagcgactg tgggagtgat cgacggagct ctcggcaacg
    14161 acgaggagct ccatcttgcc agaaagatgc gcaaacagtg cgacataatc gtggcatggg
    14221 gcgactgcgc cgtcttcggc ggaattaact gcatgaggaa cttctccgac agacagtgtg
    14281 cgctgaagga ggcctacctg gattcgccca gcaccgtcaa ccctgaggga ataatacccc
    14341 atgacgatat cccccagctt ctcccccagg ccatcccggt ggaccacgag gtgaaagtcg
    14401 acgtttacgt tccggggtgt ccgcccgatg cggacaccat tctctgggtt ttccaggaac
    14461 ttctcgaggg gcggatcccc aaggtaccct cggaaaagat tcgctacgat tagaggggga
    14521 aggaacatgg ctaccgtcaa gaagatagag atcaacccca taacccgcat cgaaggccac
    14581 gggaagataa ccgtccatct cgacgagaac ggtaacgtta acgaggcccg cttccacgtg
    14641 acccagttcc ggggtttcga gcacttctgc aagggacgag actttcgcga gatgcctgtc
    14701 attaccccga ggatatgcgg gatatgcccc gtgagccacc accttgcctc ggccaaggcc
    14761 tgtgatgccc ttctcggtgt ggaacttaca ctgacagcgc acaaactccg cgaacttatg
    14821 cacatgggac agttcgtgca gtcccatgct ctcagctttt tccacctttc cagcccggac
    14881 cttctcttcg ggttcgacgc cgacccggcg atcaggaacg ttgccggtgt gatagaaaag
    14941 tacccccggc tggctgtcaa gggagttctg ctgaggaagt tcgggcagga gatcatccag
    15001 gtactcggtg gcaagaaggt ccacccctgg atgatctcct gcccgaactt cctcagcaga
    15061 actccctt
//
LOCUS       DAOPKQ010000010        14177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_4489_length_14177_cov_4.675754, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000010 DAOPKQ010000000
VERSION     DAOPKQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4489_length_14177_cov_4.675754"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PK146_00785"
     CDS             <1..501
                     /locus_tag="PK146_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPE90802.1"
                     /translation="AGALAMVLFRIVPLRSVYTAVDWPVVILLAAMLPVAGAMASTGV
                     ADLIARSLLVTVARGKAVAALAVVLASTMVLTDFMNNAATAAVMAPISMSVALQLGVN
                     PDTFLMAVAIGASCAFLTPIGHQNNTLILGPGGFSFSDYWKMGLPTDLLVILAAVPML
                     TLVWPL"
     gene            complement(513..815)
                     /locus_tag="PK146_00790"
     CDS             complement(513..815)
                     /locus_tag="PK146_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90803.1"
                     /translation="MKMLWIICNESIGEDLMERVLDRDGVEGYTVWRDVLGKDNVGNK
                     THWGDAVFPGRNWVLMIFGSEGTIDGVIERYKTFREEPYVRKAGIKAFIHSADEVG"
     gene            complement(812..3925)
                     /locus_tag="PK146_00795"
     CDS             complement(812..3925)
                     /locus_tag="PK146_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPE90804.1"
                     /translation="MKLPEISVKRPIMTLMVFLAVLVIGSISFLNLQLDLLPKIEPPV
                     MTVITPWPGASASDVEQRITKVMENSLSMLEGVDDIISKSIDNISVVSVKFKWGVDLN
                     VRAGDVRDAVNFAKRELPSDAEESVVLRITSGTIPVVEVTMTAERSYQGLSHFVDKNV
                     VEELSRVPGVGQVLVFGGVGREIQVLLDVDRLEAFRLSPDTIAGVLERENLNIPAGSL
                     KEGQTEYFIRVPGRFSSVEEIRNTIVGIHKGNPVKIGDIAEVRDGYRELTMNGWQGDK
                     AAVVMIIMKNSDANTIEVSRMVLDRLASLREESFPSDVEYEVVMNTADFIMNAISNLG
                     RSLMAGIALVFLVTWAFLKRFRASLIVAGAIPFSLIITFIAMGRLDYTINIFTLSALA
                     MASGMVVDNAIVATDQIIFHIEQGSRRRVAAVLGASEIGSALFASTLTTIVVLLPLAF
                     LSGLVGVFFSALTVVMVLAVSASLFVSLTFIPMMASKFFGREPGKLGIHQVTERFFSA
                     MEDIYSSMLVWGLENRKKVIASALLLLLLTFLGFRTIGTELTPDPDTGDISITFSLPE
                     GTRLEVTDELVREVVSYCQETIPEAQLVFGMDGMEEGGFSVAVGQQAGPNIGTIGIKL
                     VDKNERDRSAFEIANDIRNWIRTKPGIEDMTVLVSSPIKAMFMGSKPLDIEIYGDDLK
                     QVTGVAEMIASGLAEIPGTADISVSRRQDRPELWVEPDREKAAHLGVNTAAISRTARI
                     YFAGYETSESFWEGENDFPIRVRLRGEQRNNREIFGRLTVPSGSGAPVRLSSVAMMKD
                     EVGPPQIERKNRQRYVTVGANVHGRSLGEVTVDARRMVEAMEIPEGVRVSFGGQIREQ
                     QDAFQQMGLLVLLGIMLVYMVMAGQYEAYLDPFVILFSIPFALTGVVFAFLVTGLYIS
                     MQALLGIIMLVGIVVNIAIVLVDYINLVRARGARLRDAIAEAGRRRLRPVFMTTLTTF
                     FGMLPMALSRGQGAEIWRPLAVSVMGGLLVSMLVTMLLVPVVYSIVEEKIRKQPRFVE
                     AREAARS"
     gene            complement(3922..4968)
                     /locus_tag="PK146_00800"
     CDS             complement(3922..4968)
                     /locus_tag="PK146_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE90805.1"
                     /translation="MRKTLVLILIVAIFAGLIVFRGYQQKQVKSALPEEEIARVETVS
                     LREMDLAEVVEFTASIMPEEQSAVVPKVPGRTVLEVFVSEGDRVRKGQPLASLDTSLI
                     RQQLDEARTFFETAAADHERYQSLYEDEVVSRQAADQARARYMQARSAYEQARIMSSY
                     HTITAPTDGIVARRSIDPGDTAAQGPAFLIFRQDRVKAVGAVPERQFPDIGIGDRVIL
                     SVDAIPEREFEAVVSNISPVIDPATRTGKVEVTLPSEGTILPGMFARARITSGGRKAM
                     VLPREAVSRLAGTGETACYVAEEGKAVLRIIVTGLEQEGFVEVTEGLEPGEEVITTRS
                     RNIRDGTRIEVYRR"
     gene            complement(4974..6323)
                     /locus_tag="PK146_00805"
     CDS             complement(4974..6323)
                     /locus_tag="PK146_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE90806.1"
                     /translation="MTRQRRYLAIIFVMLSLLVIALGRRAWADTGLLLDEALVVSLAM
                     ENNPQITIADKRVEQARARARGASSGKAPVLSVSALYQETYSDPQYPLVTNDPFLEGY
                     ALGGFRKTWRTALSLSWLIYSSGAVENSIRAAELSVDAVRAESQRTAQLVAHASRNAF
                     FELQRTRARASVAEEAYELAQEHRRRVEALYRNGIVAKNELLRVEVAVSEAELNLIRA
                     ESGIEVAFSALERSVGTTIRGRYRLPEPRSEPGSIEMPLDPVTAALANRPEFRALESS
                     VRSARSMAAAAVGEKGPSVYFQGDALSVGQTFYPDAMDDWKISIVAEWKLYDGGKSKA
                     RKDEALALADELLQRVEDMKHQVDLEVSVASLDLRSALQRLEVARSQVTLAEEDHRMA
                     VARYSAQVGTSIDVLDARVALSGSRTQLVDAVYDALKAWSDMKLALGEEQKTLGDKR"
     gene            complement(6326..6931)
                     /locus_tag="PK146_00810"
     CDS             complement(6326..6931)
                     /locus_tag="PK146_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPE90807.1"
                     /translation="MSDPLETREKIMQVARSHFARKGFHGTSMESIVKDASLSKGALY
                     WHFRSKGELFAAVLKKEVESIIQNYVDGPSEAEKDVNAFVTLRGERIIDMLWGDPEMR
                     LLFFTLLVERLRGGEEGRELSDIAGDLLNSIQEKLWPVAREAIPAFRNSETRNLYDKF
                     LMTLRFLMHGIIMEMGFRLDLDSAKECWRFAISRFDFKSEV"
     gene            complement(7183..8538)
                     /gene="mgtE"
                     /locus_tag="PK146_00815"
     CDS             complement(7183..8538)
                     /gene="mgtE"
                     /locus_tag="PK146_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPE90808.1"
                     /translation="MKERLDRLYSQIEDLMSEHRFPETKEALSTMEPADIGEMLVEAP
                     RAKRVLIFRLLPKDLAIEVFEHMEGAEREELLDSFTEKEAAEIIEEMSDDDRTALLDE
                     LPAKTVKKLMQHLSPEERKLANRLLNYPEDSAGRIMTAEYIGLKASMTVTEALQHIRR
                     EARSKETIYSCFVMDGERHLRGVVDLEDIILAQPETPISDLMDDDPVFVTTQTDQEKL
                     AQVMSRYDLHVIPVVDLENRLVGIVTFDDVLDIVEEEATEDFERMAGIQPIEERYLDT
                     GVFTLARKRLLWLVICIMAQAISSSVLKRYSVTIESVVALTFFIPLLIGTGGNAGMQS
                     ATLMIRGITLGDIDWKNLGHIFLREILTGLLLGIPLGLLAIGRAYMLGTGPSVALVVA
                     FALISVVMLGNLAGVALPVIARAVRIDPAVMSGPFITTTVDVVGLLVYFEIARRVLGV
                     G"
     gene            complement(8948..9343)
                     /gene="rpsI"
                     /locus_tag="PK146_00820"
     CDS             complement(8948..9343)
                     /gene="rpsI"
                     /locus_tag="PK146_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPE90809.1"
                     /translation="MKRTEYYWGTGRRKASLARVRVRSGNGTLKVNERPADEYFPRGV
                     WQTQAFEALRASGLEGKVDVFVNASGGGLTGQSGAVRLGLARALLKMNPELKPTLRKF
                     GLLTRDPRMVERKKFGQKGARGKRQYSKR"
     gene            complement(9374..9805)
                     /gene="rplM"
                     /locus_tag="PK146_00825"
     CDS             complement(9374..9805)
                     /gene="rplM"
                     /locus_tag="PK146_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPE90810.1"
                     /translation="MAVNSTYMAKKEQVERKWFVVDATDKVLGRLAAQVAMILQGKNK
                     PTYTPHVDTGDFVIVINADKIKITGRKLERKYVYRHSGYKGGLKATSYGTFMEKYPDR
                     LFERVVKGMLPKNRLHFEKKLKVYAGPEHPHTAQKPEPIEI"
     gene            complement(9953..10993)
                     /locus_tag="PK146_00830"
     CDS             complement(9953..10993)
                     /locus_tag="PK146_00830"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPE90811.1"
                     /translation="MDSPRLNRLSDVAAFEPGTARLLSATHEEILSGKTTDVYFVKTR
                     DILDACGRLDVPVVAEIFARHGGVFAGISEVVRLLEGSGVEILSLKEGDTFSPREVVT
                     RISGPYSAFGMYETVILGMLASSTGWATAARECVDAAGGRPVLSFGARHVHPAVSSVM
                     ERAAVVAGGCVGASCILGAKLAGIEPRGTIPHAAILLAGDTLQIAKVYDDFLPDGEPR
                     IILVDTFHDEAEESLRIASELGKRLSGVRLDTPSERGGVTPELVREVRHRLDMAGASH
                     VDIIVSGGINPERIKVLSEAGAASFGVGSYIAHAAPRDMTMDIKVVDGKPLAKRGRIP
                     GITENPFLERIL"
     gene            complement(11010..11237)
                     /gene="secG"
                     /locus_tag="PK146_00835"
     CDS             complement(11010..11237)
                     /gene="secG"
                     /locus_tag="PK146_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPE90812.1"
                     /translation="MKVFLTVVHVIVSISVMAVILMQQRKQGGFTGVFGGGTQADAAG
                     GQWQRFSVLTKATIVLMAVFLVTSLILVLMS"
     gene            complement(11279..11878)
                     /locus_tag="PK146_00840"
     CDS             complement(11279..11878)
                     /locus_tag="PK146_00840"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPE90813.1"
                     /translation="MPGIVFASWNRNKHLEMSSLLAPAGVDLLFGPDLAGAGEVPEVD
                     EDRDTYAGNAVIKALAWSLALGRPALADDSGLEVSSLNWGPGVMSARVAPDDAARIQW
                     LLGKMEGISERRARFVAALALFFPERGIWLLAGGSCYGSIAVEPSGIYGFGYDPLFVP
                     QGYNETLAVLGPTVKSRISHRSVAARALSRMLTGGCVVE"
     gene            complement(11841..12650)
                     /gene="rph"
                     /locus_tag="PK146_00845"
     CDS             complement(11841..12650)
                     /gene="rph"
                     /locus_tag="PK146_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPE90814.1"
                     /translation="MTRATEEGRGSRIDGRAPGDLREISILRGCSRFAEGSALVDFGA
                     TRVLCTASVEEKVPLFLRGSGKGWVTAEYSMLPRSTEIRTPRDSVRGGISGRSHEIQR
                     LIGRSLRAGTALELLGERTVWIDCDVLQADGGTRTAAITGGFVALVDALRWLFARRCV
                     DTIPLKCFVAALSVGKVDGEIVADLCYGEDSRAEVDFNIVMNEFGDFIEVQGTAEGEV
                     FSRKELVEMLDLAGSGISSLIDIQKSALGFTGEEERELAVARDRIRQLEQK"
     gene            complement(12647..13345)
                     /locus_tag="PK146_00850"
     CDS             complement(12647..13345)
                     /locus_tag="PK146_00850"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPE90815.1"
                     /translation="MPDFRDWEKRAPRPLFEDDEVDDLYDEDEDDDDGDKAPLVLRIV
                     AWISVLVLLFAGGYWGTSLTLKYLDKKQIIGQHNVVSDPDEARRVAEDTSDPTGLAVR
                     RSGFEVFVPRGDVLEGETVSLVPGILEDDVKTVISGLLETMRAENTISEQVRVLHVFR
                     NGDLLYLDLNDPFVRAVQNLPGEKGALIMTSLVRTIVQNFSPVTRVRILINGKDPELK
                     TPVDLTMPWQLKTS"
     gene            complement(13395..>14177)
                     /locus_tag="PK146_00855"
     CDS             complement(13395..>14177)
                     /locus_tag="PK146_00855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPE90816.1"
                     /translation="PPEGTIVEEEPPEEVFQPLPQPVQPSGRSPGGAKSVVIDAGHGG
                     KDPGAVANGIREKDINLRVARLMSDILKKEGLRVVLTRSTDVYLQLSQRTNIAVKEDA
                     DVFVSLHCNALPKGRTAEGVEIYLMALPSDKHAMELALIENRELVEGGLESSEAADKR
                     TRTLLKILGDMQQNVKIEESTSFAEILFRKGKSRKLPMRRVAQAPFYVLRGAAMPSVL
                     VEMGFLTNRAEAAKLKNPSYQKQIAEALSSGIVEFLKTSEYN"
BASE COUNT         2818 a         4470 c         3745 g         3144 t
ORIGIN      
        1 gctggcgccc tggcaatggt ccttttcagg atcgtacctc tccgctccgt ctacaccgcc
       61 gtggactggc cggtggtgat cctcctggcg gccatgctcc ccgtggcagg agccatggca
      121 tccaccggtg tggcggacct gatcgcccgg tccctgctgg ttaccgtagc aaggggtaag
      181 gccgtagccg cccttgccgt tgtcctggcc tcgacaatgg tcctgacgga cttcatgaac
      241 aacgccgcca ccgcagctgt tatggcgccc atatcgatga gcgtcgccct ccagctgggt
      301 gtgaaccccg acactttcct gatggccgtg gccataggcg cctcatgcgc cttccttaca
      361 cccatcgggc accagaacaa caccctgatc ctgggaccgg gggggttctc cttcagcgac
      421 tactggaaaa tgggactccc gacagacctg ctggtgatac tggccgctgt accgatgctc
      481 acgctggtct ggccgcttta gccagaccgg acctagccga cctcatcggc gctatggatg
      541 aaggccttga taccggcctt tctgacgtag ggctcctccc tgaaggtttt gtagcgctcg
      601 ataaccccgt cgatagtccc ttcggagccg aagatcatta agacccagtt ccgtccgggg
      661 aaaacggcat caccccagtg ggtcttgttc ccaacgttat cctttcccag cacatcccgc
      721 cagacggtgt acccctccac accgtccctg tcgagcaccc tttccatgag gtcttctcct
      781 atgctttcat tgcaaatgat ccatagcatc ttcatgatcg agcagcctcc ctggcctcca
      841 cgaacctggg ctgttttctg atcttctctt cgacaatgct gtagacaaca gggaccagga
      901 gcatggtcac aagcatggag acaaggagac cacccatgac cgagaccgcc agggggcgcc
      961 aaatctccgc tccctgccca cggcttaaag ccatgggcag cattccgaag aaggttgtca
     1021 gggtggtcat gaagaccggc ctgagacgcc ttcgtcccgc ctcggctatg gcgtccctga
     1081 gacgggcacc cctggccctg accaggttga tgtaatccac cagcactata gctatattga
     1141 ccacgatacc cacgagcatg atgatcccca gcagggcctg catggatatg tagagccccg
     1201 tcacgaggaa ggcgaagacc actcccgtta gagcaaaggg aatgctgaag aggatgacga
     1261 agggatccag gtaggcctcg tactgacccg ccatgaccat gtagacaagc attatgccga
     1321 gcaagaccag aagtcccatc tgctggaagg catcctgctg ttcccggatc tggcctccaa
     1381 aggataccct gacaccctcg gggatctcca tggcctctac catcctcctg gcatcgacgg
     1441 tcacctctcc cagagaacgc ccgtgaacgt tggcgccgac ggtgacgtac ctctgcctgt
     1501 tcttgcgctc gatctgcggc gggccaacct catccttcat catcgccaca gaagaaagcc
     1561 ggacaggagc ccccgatcct gatggcacgg tcaacctgcc gaatatctcc ctgttgttcc
     1621 tctgttctcc tctgagcctc accctgatgg ggaagtcgtt ctccccctcc cagaaggatt
     1681 cgctggtctc gtaaccggcg aaatagatcc tcgccgtacg tgagatcgcg gcggtgttca
     1741 cgcccaggtg ggcagctttt tccctgtcgg gttccaccca tagttccggc cggtcctgcc
     1801 tgcggctgac agagatgtcc gccgtgccgg gtatctcggc aagccccgac gcgatcatct
     1861 ccgcaacccc agtcacctgt ttcaggtcgt cgccatagat ttctatatcc agcggctttg
     1921 agcccatgaa catcgccttg atcggagaac tgaccagcac ggtcatatcc tcgatgcccg
     1981 gcttggtacg gatccagttc cggatatcgt tggctatttc gaaggcggac ctgtcccttt
     2041 cgttcttgtc cacaagcttg atccctatgg tgccgatgtt gggtccggcc tgctgaccta
     2101 ctgcgacgga gaagcccccc tcctccattc cgtccattcc gaagaccagt tgagcctcgg
     2161 gaatggtctc ctggcagtag gagaccacct ccctgaccag ttcatcggtt acctccagcc
     2221 tggtaccctc gggaagggag aaggttatgg agatgtctcc cgtgtccgga tccggggtaa
     2281 gttcagtgcc gatggtgcgg aaccccagga aggtgagaag gagcaacaga agagccgatg
     2341 cgatgacctt ctttcggttc tcaagacccc agaccagcat ggatgaatag atatcctcca
     2401 tggcggagaa gaatctctca gtgacctggt gtatccccag ttttcccggc tcccgcccga
     2461 agaacttgct cgccatcatg ggaatgaagg taaggctcac gaagagcgat gccgatacgg
     2521 ccagcaccat gacaaccgtg agggccgaga agaaaacacc taccagtccg gataaaaagg
     2581 ccaggggcag gagaaccact atggtcgtca gcgtcgaagc gaacaatgca gagccgatct
     2641 cgctggctcc caggaccgcc gcgacccggc gtcggctgcc ctgctctatg tggaagatga
     2701 tctggtcggt ggcgacgata gcgttgtcca cgaccatacc gctcgccatg gcgagagccg
     2761 agagggtaaa tatgtttatc gtgtagtcaa gacgccccat ggcgatgaag gtgatgatca
     2821 gcgagaaggg tatggccccg gccacgatca gtgatgccct gaatctcttc aggaacgccc
     2881 aggtgacgag aaagaccagc gcgatccccg ccatcaggga ccttccgagg ttggatatgg
     2941 cgttcatgat aaagtcggcg gtgttcatga ccacctcgta ctcaacgtca gaggggaagc
     3001 tctcctccct gagagaggcc agcctgtcca gtaccatccg ggagacctcg atggtgttgg
     3061 cgtcactgtt cttcattatt atcattacga cggcggcctt gtcgccctgc cagccgttca
     3121 tggtcaactc cctgtatccg tcccttactt cggcgatgtc gccgatcttg acggggttgc
     3181 ccttatggat ccccacgatg gtgttcctta tctcctccac cgatgagaac ctgcccggca
     3241 ccctgatgaa atattccgtc tgtccttctt tcagggaacc ggcagggata tttaggttct
     3301 ccctctcgag aacgcctgct atggtatcgg gtgacaaacg gaaggcctcg agcctgtcga
     3361 catcgaggag gacctggatc tcccttccga cgcctccgaa gacgaggacc tggcctactc
     3421 ccggaaccct ggacagctcc tcgacgacgt tcttgtcaac gaagtgggag agcccctggt
     3481 aggacctttc ggcggtcatg gtgacctcca cgacggggat agtccccgac gttatcctga
     3541 ggacaaccga ctcctccgca tcggagggaa gttccctctt ggcaaagttg acggcatccc
     3601 tcacgtcccc ggcccggacg ttcagatcca caccccattt gaacttaacc gagaccacag
     3661 agatgttgtc gatggacttg gagattatat cgtccacccc ctccagcatg gagaggctgt
     3721 tctccataac cttcgttatc ctctgctcca catcgctcgc cgaggctccg ggccaggggg
     3781 ttatcactgt catgacgggc ggctcgatct tcggcagaag atcgagctgc aggttgagga
     3841 aagatatgga acctatgacc aggacggcga gaaagaccat caaggtcata atggggcgtt
     3901 tgacggagat ctcggggagc ttcaccttct gtacacctct atcctggtcc cgtccctgat
     3961 attcctggac ctggtggtga tcacttcttc gccgggctca agaccttcgg tgacctctac
     4021 gaaaccctcc tgttccagtc cggtgacaat gatgcgaaga acggccttgc cttcttctgc
     4081 aacgtagcac gcagtctcgc cggtaccagc caggcggctg acagcctcac gggggagcac
     4141 catggccttt ctcccgccgg aggttatcct ggccctggcg aacattccgg gcaggatggt
     4201 cccttcggag ggtaaggtca cctctacctt acccgtacgg gttgcgggat ctatgacggg
     4261 ggagatattg gagacaacgg cctcgaactc cctttccggg atggcatcca ccgagagaat
     4321 gaccctgtct ccgataccta tgtccgggaa ctgcctttcg gggacggcac ccacggcctt
     4381 tactctgtcc tgcctgaaga taaggaaggc cggcccctgc gcggccgtat cgccggggtc
     4441 gatggaacgc cttgccacaa tgccatccgt cggagctgtt atggtatgat agctgctcat
     4501 tatcctggcc tgctcgtagg cggatcttgc ctgcatgtaa cgagctctgg cctggtctgc
     4561 agcctgtctg cttaccactt catcctcgta tagggactgg tagcgttcgt ggtcggctgc
     4621 tgcggtctcg aaaaaggtcc tggcctcatc gagctgctgt ctgatcaggc tggtgtcgag
     4681 ggatgccagt ggctgcccct tcctgaccct gtccccttcg gagacgaata cctccagaac
     4741 ggtcctcccg ggaaccttgg gaacgacggc cgactgctcc tcgggcatga tgctggcggt
     4801 aaactccaca acctcggcaa ggtccatctc ccggagtgag acggtttcca cccttgcgat
     4861 ctcctcttcg gggagagcgc tcttgacctg tttctgctga taccccctga aaacgataag
     4921 tcctgcgaag atcgcaacaa ttaatatcag aaccagcgtc tttctcatgt ctatcacctt
     4981 ttatccccga gggtcttttg ttcctcacca agtgccagtt tcatgtcgga ccaggccttc
     5041 aacgcgtcgt agacggcgtc gacgagctgg gttctcgatc cggagagggc caccctggcg
     5101 tcgagtacgt cgatgctagt ccccacctgg gccgagtacc ttgccacagc cattcggtga
     5161 tcctcctccg cgagggtcac ctgtgacctg gcaacctcca ggcgctgcag ggccgatcgg
     5221 agatcgaggg aagctaccga tacttccagg tccacctggt gcttcatgtc ctccacccgc
     5281 tgcaaaagtt cgtcagccag agcaagggct tcatcctttc gggcctttga cttgcctcca
     5341 tcgtagagtt tccattccgc cacaatggag atcttccagt cgtccattgc gtcggggtag
     5401 aaggtctgcc ccaccgataa ggcgtctccc tggaaataaa ccgagggccc cttctctccc
     5461 acggccgctg cggccatgga ccttgccgat cgcaccgaag actcgagagc cctgaactcg
     5521 gggcggtttg ccagggctgc tgtaacaggg tccagaggca tttcgatgga gcccggctca
     5581 ctccgcggtt cggggagccg gtacctgcct ctgatggtcg tgccgacaga cctttcaaga
     5641 gcgctgaaag cgacctctat gccgctctcc gcccggataa ggttaagctc cgcttcggag
     5701 acggccactt caaccctcag gagctcgttc tttgccacta ttccgttcct gtagagggcc
     5761 tcgaccctcc tcctgtgctc ctgtgccagc tcataggcct cttcggccac ggaggccctg
     5821 gcccgagtcc tctggagttc gaagaaagcg ttccttgacg cgtgggcaac gagctgggcc
     5881 gtcctctgcg attcggccct taccgcatcg acggagagtt cggcggcacg gatgctgttc
     5941 tccacagcac cgctactgta gatgagccag gagagggaaa gggccgtcct ccaggtcttg
     6001 cggaatccac caagagcata accttcaagg aatggatcat tggtcaccaa agggtattga
     6061 ggatcgctgt aggtctcctg gtaaagagcg gagaccgaaa gtacgggagc cttccctgag
     6121 gatgcccccc ttgccctggc tcttgcctgt tcaactctct tgtcggcgat agttatctgg
     6181 gggttgttct ccatcgcaag tgataccacc agggcctcgt ccagcaggag ccccgtatcg
     6241 gcccatgccc ttcttcccag ggcgatcacc agaagggata acatcacaaa aataattgca
     6301 aggtatcttc tttgccgcgt catctttaaa cctcgctttt gaaatcgaaa cggcttatgg
     6361 cgaaccgcca gcactccttc gccgaatcaa gatccagacg gaaacccatc tccatgatta
     6421 ttccgtgcat aaggaaccgc agggtcataa ggaatttatc gtagaggttt ctggtttcgc
     6481 tgttgcggaa ggccggtatg gcctcccggg caacaggcca cagtttctcc tgaattgaat
     6541 tcaggaggtc ccccgcgata tcagaaagtt ccctgccctc ttctccacct ctgagcctct
     6601 ctactaaaag cgtgaaaaag agcaggcgca tctccggatc accccagagc atgtcgataa
     6661 ttcgctctcc tcgaagggtg acgaaggcat ttacgtcttt ctcggcttcc gaggggccat
     6721 caacgtagtt ctgaatgata ctttcaacct ctttcttgag gactgctgca aaaagctccc
     6781 ccttgctcct gaaatgccaa taaagggccc ccttgctcaa ggatgcgtcc ttgactatgg
     6841 actccatgct ggtcccgtgg aaaccctttc tcgcgaaatg gctacgggct acttgcatta
     6901 ttttctccct tgtctccaaa ggatcggaca tcccatctcc tccagaccga acaggctgca
     6961 gaacagcaag gaggctatca cagaccgacc ggtctgtcaa tccagaaccg taagcgtgag
     7021 tcgaaaaccg caacaaaaag gccggaggca ttttcgcttc cggcactgtg aattcatctt
     7081 gtggatccat gaaccatacc tttcggtttt tcccttcatc gtccactgtt tacacccttg
     7141 atcttaaggc ccacgtttta cgaatcacgc atcacggggt ctttacccta ctccgagcac
     7201 tcggcgggcg atctcgaagt agaccaggag tcccaccacg tcgacggtgg tcgttatgaa
     7261 ggggcctgac atgaccgccg ggtcgatcct tactgccctc gctattaccg gaagagccac
     7321 cccggcaagg ttgcccagca tgaccaccga gataagggca aaggccacca ccagcgccac
     7381 gctcggccct gttccgagca tgtaggccct cccaatggcc agaagcccca gagggatccc
     7441 caacagaagc cctgtcagta tctccctcag gaatatatgg cccaggttct tccagtctat
     7501 gtcccccagc gttattccgc gtatcataag agtggccgac tgcataccgg cgttcccccc
     7561 ggtacctatc agcagcggga tgaagaaggt cagggccacc acgctctcga tggtaaccga
     7621 atatctcttg agaaccgaag agcttatcgc ctgggccatg atgcatatga ccagccagag
     7681 aaggcgcttc ctcgcaagtg tgaagacccc cgtatcaagg tacctctcct ctatgggctg
     7741 tataccggcc atgcgttcga agtcctcggt ggcctcctcc tcgacaatgt caagaacgtc
     7801 atcaaaggtt actatcccca caagacggtt ctcaaggtcc accacaggga tcacgtgaag
     7861 gtcgtagcgc gacatcacct gggccagctt ctcctggtcc gtctgcgtcg taacgaacac
     7921 cgggtcatcg tccatcaggt ccgatatggg cgtctcgggc tgcgccagta ttatgtcctc
     7981 caggtccacg accccacgaa ggtggcgctc accgtccatc acaaaacagc tgtatatggt
     8041 ctccttgctc cgagcctccc tccgtatgtg ctgcagcgcc tccgtcaccg tcatcgaggc
     8101 cttcagccct atgtactctg ccgtcatgat cctgccggca gaatcctcgg ggtagttcag
     8161 aagccggttc gccagcttcc tctcctcggg agaaaggtgc tgcatcagct tcttaaccgt
     8221 cttcgccgga agttcgtcaa gaagggccgt acggtcatca tcggacatct cctcgatgat
     8281 ctcggccgcc tctttctcgg tgaagctgtc aagaagctcc tctctctccg ctccctccat
     8341 gtgctcgaac acctcgatgg caaggtcctt cggcaacaga cggaatatga ggaccctctt
     8401 ggctcggggc gcctccacca gcatctcgcc tatgtcggca ggctccatcg tagacagtgc
     8461 ctccttggtc tccgggaacc tgtgttccga catgaggtcc tctatctggc tgtaaagacg
     8521 gtccagtctc tctttcatgg ctgattcacc cccgttcggc ctgaccagga acaacaaccg
     8581 gcaccacccc aacctcccta agttaccggg ggtcgatacg cgggtacccc ttggtctctc
     8641 tggtatttgc cttttctctg ttctgagggt gatcagttcg gctggaagga tatcctgctg
     8701 ccgggaggag ttatgaggaa aaagtgcggc ttcctaaccg tgcccggaga tcaccctctc
     8761 ttctgcggta caatcgtctt gggaaccggt actatggcac tcttccattt tcatctcctt
     8821 cgttcctgtc gcatggacct gcaatttcat cctatactcg gcagacagcg cggtcaacag
     8881 gaaaaaaagg acgcccgcct taaggcgggc gtcctgatct atctgttgca ggcaaaaccg
     8941 cggtaaatta acgcttcgag tactgcctct ttccgcgggc gcctttctgg ccgaattttt
     9001 tcctctcaac catcctgggg tccctggtaa ggagcccgaa cttcctgagt gtcggtttaa
     9061 gctcggggtt catcttgagc agagcccttg cgaggcccag cctgacagca cctgactgcc
     9121 cggtcagacc gcctccgctg gcattaacga agacatcgac cttgccctca agtcccgacg
     9181 ccctcagcgc ctcgaaggcc tgtgtctgcc atacgccacg agggaagtat tcatcggcag
     9241 ggcgctcgtt gaccttcagg gtcccgtttc cggaacgcac cctcaccctt gcaagggagg
     9301 ccttgcgtct cccagtaccc cagtaatatt cggttcgctt catcttcttt tccctccctt
     9361 ccctgtggcg ttcctagatc tctatcggct ccggcttctg agctgtatgc gggtgttctg
     9421 gaccggcgta gaccttaagt ttcttctcaa agtgcaaacg gttcttggga agcattccct
     9481 tgacgaccct ctcaaaaagg cggtcggggt acttctccat gaaggtgccg taagaggttg
     9541 ccttcagccc gcccttgtat ccgctgtggc gatagacgta cttcctctca agtttcctcc
     9601 ccgttatctt tatcttgtcg gcgttgatga cgatcacgaa atcgccggta tccacgtggg
     9661 gagtataggt tggcttgttc ttgccctgga ggatcatggc aacctgggcg gccagccttc
     9721 cgagcacctt gtcggtggcg tcaacgacga accattttct ctctacctgt tccttctttg
     9781 ccatgtaggt tgaatttaca gccatttctt gtgttcctct ccttatcgcc acgccgccgg
     9841 aagcagcgga cacaataatt taccttccct gcagacaccg caaaaacgca gagcggtgct
     9901 tattctatct aagcgccccg tgggtgtcaa ggacgactga tgccggaacc tttcagagaa
     9961 tcctttcgag gaaggggttc tctgttatgc ctgggatcct ccccctcttg gccagaggtt
    10021 tgccgtctac caccttgata tccattgtca tatctctcgg tgccgcatgg gcaatataac
    10081 tcccgacacc gaaggatgcg gcgcccgcct cggaaaggac ctttatcctt tcggggttga
    10141 tcccgcccga tacgataata tccacatggg aggcaccggc catgtccagc ctatggcgaa
    10201 cctcacggac cagttccggc gtcactcctc cccgctccga gggggtgtcg agcctgaccc
    10261 ccgatagcct tttccccaat tccgacgcta tcctgagcga ttcctcggcc tcatcatgaa
    10321 aggtatccac aaggatgatc cggggctccc catcggggag aaagtcatcg tagaccttcg
    10381 ctatctggag tgtgtcgcct gcaagaagta ttgcggcgtg agggatggta cccctgggct
    10441 ctattcctgc aagcttcgct ccaagaatgc agctggcgcc cacacagcct ccggctacaa
    10501 cagcagccct ctccattacc gacgagacgg cggggtgaac atgccttgcg ccgaaggaga
    10561 gtacgggcct gcctcccgcg gcatccacgc attctctggc agcggtggcc caacccgtcg
    10621 aactggcaag cattcccagg atcaccgtct cgtacatgcc aaaggccgag tagggaccgc
    10681 tgatcctggt cactacctcc ctggggctga aggtgtctcc ttccttgagg gagaggatct
    10741 ccacgccgga accttccagg agcctgacca cctcggagat cccggcgaac actccgccgt
    10801 gcctggcgaa gatctccgcg accacaggta cgtccagcct tccacaggca tcgaggatat
    10861 ccctggtctt gacgaaatag acgtcggtgg tcttgccgga caggatctcc tcgtgggtcg
    10921 cactcaagag ccttgccgtc cctggctcaa aggcggccac gtcggagagc ctgttgagtc
    10981 tcggggagtc catcgccctc accccgggat caggacatga gaaccaggat caatgatgtt
    11041 accaggaaga cggccattag aacgattgtc gccttggtga ggacggagaa cctctgccac
    11101 tgtccccctg cggcatcggc ctgggtcccg cctccgaaaa caccggtgaa gccgccctgc
    11161 ttgcgctgct gcatgaggat aaccgccatc accgagatcg aaacgatcac gtgaacgacc
    11221 gtgaggaaaa ccttcacgcc ttgcacctcc cagatgattc ttggcctgaa acgaagattt
    11281 attctaccac acaaccgcct gtcagcatac gggaaagggc tcttgcagct acagacctgt
    11341 gggatattct cgattttacc gttggcccga gaactgcaag cgtttcgttg taaccttgag
    11401 gcacgaagag gggatcgtag ccgaaaccgt agatccccga gggctccacg gctatgctgc
    11461 cgtagcagga acctccggca aggagccata tacccctttc agggaagaaa agcgccaggg
    11521 ctgccacgaa tcgggctctc ctttcagaga tcccttccat cttgccaaga agccattgga
    11581 tcctcgctgc atcgtctggg gcgacccttg ccgacatcac tcccggcccc caattgaggg
    11641 agctgacctc tagcccgctg tcatcggcca gggcaggcct gccaagggcc aatgaccatg
    11701 ccagggcctt gatgacagcg ttgccggcgt aggtatccct gtcctcgtcg acttcaggaa
    11761 cttcccctgc accggcaagg tcggggccga agagaagatc gacacccgcg ggggccagga
    11821 gagagctcat ctcgaggtgc ttatttctgt tccagctggc gaatacgatc cctggcaact
    11881 gccagttccc tctcttcttc cccggtgaac cccagggcac ttttctggat atctatcagg
    11941 gatgaaatac cacttccagc cagatccagc atctcaacca gctcttttct ggagaagacc
    12001 tccccttcgg cggtcccctg cacctctatg aagtcgccga attcgttcat gacgatgttg
    12061 aagtccacct cggcccggct gtcctctccg taacagaggt ccgcgacaat ttcaccgtca
    12121 accttgccta cgctcagggc ggccacgaag cacttcaatg gtatcgtatc gacgcacctc
    12181 ctggcgaaaa gccacctgag ggcgtccacc agggccacga aacctccggt aatggcggca
    12241 gtcctggtcc caccgtcggc ctggagcaca tcgcagtcga tccagactgt gcgctctccc
    12301 agaagttcca gggctgtacc tgccctcagg gaccggccga taagcctctg gatctcgtgg
    12361 ctcctgccgc tgatgccccc cctgacgctg tcccggggag tcctgatctc ggtggacctg
    12421 ggcagcatcg agtattcggc tgtcacccag cctttgccgc tccccctcag gaacaggggc
    12481 accttctcct ccaccgaggc cgtacagagt acccttgtcg ctccgaaatc caccagggcc
    12541 gacccctcgg cgaacctgct gcaccctctc aagatggaga tctctctgag gtcgccgggg
    12601 gcacgcccgt cgatccttga tcccctaccc tcttcagtcg cccgggtcat gaggttttca
    12661 gctgccaggg cattgtaagg tccacggggg tcttgagctc cgggtccttg ccgtttatca
    12721 ggatgcggac cctggtgacg ggcgagaagt tctgtactat ggtccttacg agggacgtca
    12781 tgatcagcgc gcccttttca ccgggaaggt tctggaccgc cctgacaaag gggtcgttaa
    12841 ggtccaggta gaggagatcg ccgttacgga aaacgtgaag gacccggacc tgttcagaga
    12901 tggtgttctc agccctcatg gtctccagaa gaccggagat gacggtcttg acatcatcct
    12961 caaggatccc ggggacaagg gagaccgtct ccccttcaag aacatctccc ctggggacga
    13021 acacctcgaa acccgatctt ctaactgcaa gaccggtggg gtctgaagta tcctccgcta
    13081 cccttcttgc ctcgtcgggg tcgctcacca cgttgtgctg cccgattatc tgtttcttgt
    13141 ccaggtattt cagggtcagc gaagttcccc agtaaccgcc ggcgaaaagg agcaccagaa
    13201 cggagatcca ggcaacgata cgaaggacca gcggagcctt gtccccgtcg tcatcatcct
    13261 cgtcctcgtc atagaggtcg tccacttcgt catcttcaaa aagaggccga ggagcccttt
    13321 tctcccaatc ccggaaatcc ggcatagcat tcccctccga tcgcaatttg tcttttcagg
    13381 gcgctggaca gcgcctaatt gtattcgctc gttttaagga attccacaat ccccgaagag
    13441 agagcctcgg cgatctgctt ctggtagctg gggttcttga gcttcgcagc ttccgccctg
    13501 ttggtcagaa aacccatctc taccagcacc gatggcatcg ccgctcccct gagaacgtag
    13561 aacggagcct gcgcgaccct cctcatggga agcttcctcg attttccctt cctgaaaagg
    13621 atctccgcaa aggaggtgct ctcctctatc ttcacgttct gctgcatgtc tcccaggatc
    13681 tttagcagag tccgtgtcct cttgtccgcg gcctcgctgc tctccaggcc tccctcgacc
    13741 agctccctgt tctctatcag ggccagttcc atggcatgct tgtcgctggg caaagccatc
    13801 aggtatattt ctacaccctc ggctgtcctc cccttgggca gggcgttgca gtgaagactg
    13861 acgaagacgt cggcgtcctc ctttacagct atgttcgttc tctgagagag ctgcaggtat
    13921 acatcggtgc tcctcgtgag gaccaccctc agaccttcct ttttaaggat atccgacatg
    13981 agcctggcca cacggagatt aatatccttc tccctgatcc cgttggcaac ggcacccggg
    14041 tcctttccac cgtgccctgc atcgatcact accgacttcg ccccgccggg ggatcttcca
    14101 gagggctgta ctggctgggg aaggggctgg aacacctctt cggggggttc ttcctcgaca
    14161 atggtccctt caggggg
//
LOCUS       DAOPKQ010000011        14130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_4515_length_14130_cov_5.452718, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000011 DAOPKQ010000000
VERSION     DAOPKQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4515_length_14130_cov_5.452718"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..855)
                     /locus_tag="PK146_00860"
     CDS             complement(103..855)
                     /locus_tag="PK146_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE90817.1"
                     /translation="MSSLEKSLMILKRLGDSPYELNLSELASHVGMGKSGAFKLINVM
                     KKHNFVIQDPSSKKYHLGPIMLRLGNVYSRFKGIEQIAKPLLFRISRSFQETTYLTIW
                     EGDRAFPAYKNSVKDGIYEYNDFIGMSLPLNSGASAMVLCAYQDPGHIREILGSMKLE
                     KRTSKTMTDLDQLMDEYRIIRERGYALEDETFSLGILSIAVPVFDKDGRVWSCLALAA
                     PKAHATDGNVDEWIQALKEGAEELSYNLQFRH"
     gene            1054..2178
                     /locus_tag="PK146_00865"
     CDS             1054..2178
                     /locus_tag="PK146_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE90818.1"
                     /translation="MKIEPFGVEQWMNRWETLAVNNIAETCVDSLLLYELLEMSGRKE
                     EVMVDLLGTRMTYGDIKGSERLRGLIGELYEDRDPERVLIMNGGSAANFLALFTIVGP
                     GERVISVWPTYQQLTSIPKAFGAKVDILQLRPENFWLPDTDELEKMAESGVDLICLNN
                     PNNPTGSLMDRETLERIAVIARKHGAWVLCDEAYRGLVHEPGVSVSSMADIYEKGVST
                     GSMSKVFSLAGLRSGWMTGPREFIEEAFSRRDYTTISCGPLVDALATVALENRDKITE
                     RNLKIIRESVGLLGEWIDSEPLFHWVKPTAGTTAFVGYDLDIPSDEFCVDLLGTTGTF
                     LLPGSCFGIDKRLRFGYAYGTDALKRGLESLSGWVRERES"
     gene            complement(2482..3342)
                     /gene="dapF"
                     /locus_tag="PK146_00870"
     CDS             complement(2482..3342)
                     /gene="dapF"
                     /locus_tag="PK146_00870"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPE90819.1"
                     /translation="MNTSGEGILFTKMHGNGNDFLIIDNRLGEIPGHELPDLALRLCR
                     RRTSIGGDGLMAVERSDRGDIRMRLFNCDGSEGEMCGNGARCLARYAFEKGMAPARMT
                     IDTLAGPVVAEVEGVFATLDMGVLETASALRGQVLILSGERLVYSQLNVGVPHTVIFQ
                     DPGDERAEMDLARIARAFQSDRERFPQGTNVNFMKIRTTGEIDLRTYERGVNDFTMSC
                     GTGSTAGAVAAWLEGKVSTPVEVRNPGGVNTVLIDEEGPGRVRLFLKGQTTAVAEGVV
                     SGECLQIREP"
     gene            complement(3339..4361)
                     /locus_tag="PK146_00875"
     CDS             complement(3339..4361)
                     /locus_tag="PK146_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE90820.1"
                     /translation="MTSDWTRSQQSVSGFERAMALAGAATLKEALPICLEALIGSSEG
                     SLDCGWVYLVPEKAEIFRLRCSIGVSDPFVEANASWAPDSVHGRAIFRDTPWYGSFSE
                     TLNGQDPLLASEGLHAFAILPLFRGSSVAGCFCLASHDSDTVPQHVKRDLEALSRWFD
                     KTIARVQVSESRIRESWELLSLFDAMAEMVFAFDLDGEIVWINRTAREELGLDNNGRI
                     HLLDLHPDDTRPEAARIFRRMLSREQQTSNVPYAKRDGSVVHATTLGVPGRWKGEDVL
                     FGISRIHQTGGRTSVEAAAEVSSLERDIARMAMVLDERLSRIEEALMISPESSASRLW
                     TGSIKE"
     gene            complement(4522..5469)
                     /locus_tag="PK146_00880"
     CDS             complement(4522..5469)
                     /locus_tag="PK146_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90821.1"
                     /translation="MELDFIKGHMGGNTILLFNGRQVRRESHVLETALTVLDRKRLYA
                     DQAAFLYPSGKANTIRALVVDITNRNFIPACGGFTQVLGKALVDTDLVKWFKVKTAPQ
                     EFEVTLELESGPSTVTVHKTKGGKFRKAESDMTSFARMLAGDGVMPVDLDGIQAFKSG
                     YYLVQEAPKLKEHYPEADFEEMNTAAVKAISEAQAFFHQKGYSSTLHFSLFDRSPAHK
                     GHLRAVFPHGVLTGNIEPTCGTGSTALALALLLTGEGKDLGLVKGGRIKVRLETGGGP
                     ALGGPDITTVVVEKKGEEVTRAFFSHSLVEITAKGTVFV"
     gene            complement(5776..6969)
                     /locus_tag="PK146_00885"
     CDS             complement(5776..6969)
                     /locus_tag="PK146_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HPE90822.1"
                     /translation="MKRPVLILISAIIMTALGCGTALGAGFALYDWSARGNALGGAMT
                     GRADDPSAIAFNPAGITQIPDSSMMAGLAFIMPGGTLTEIGGDSVGMIDHTFIPPHFY
                     YTRQLSDRLWFGVGLMTRFGLGTDFPPDWYGRYNSYYANIQSVSINPNLAWKVNDRLS
                     LSVGVEALWFEYGARRFIGTDFRLLGDDIGYGWNVGLHYKLDEATRIGLHYRSQVKLT
                     ISGTADIQGVGSTDAWGDLTLPEMFLFGLSRQVTPKLNVEVGAVYTGWSSYDRLAIHF
                     ENDFGMLPNPSTSVKNWKNVWRYQLGLEYRYSPEWTWRFGYTYDQEPSPDGTVDFQAP
                     MSDRQLFSIGFGYTKNDRTWDFAYTYLLADDRTIAGRPGDRIPDSRTSNMDAHIFMIS
                     YSVRF"
     gene            complement(7024..7653)
                     /locus_tag="PK146_00890"
     CDS             complement(7024..7653)
                     /locus_tag="PK146_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPE90823.1"
                     /translation="MRDKEETRKKILEAVGEILSEEGFGGIGVNNVSRRAGVDKVLIY
                     RYFGDLPGLLREFASGGDYWPSMEDLVEKAEGDNRPPGASELAVALLKGYIRELRDST
                     SAQELLLGELVSRNELSEASARKRERQGADLIEALVRSAPEAERVDVAALGALLSAGL
                     TFLMLRARTSEAYLGIDISSEEGWGRIERAMEELVRGVMGDDKLEGFQY"
     gene            7812..9398
                     /locus_tag="PK146_00895"
     CDS             7812..9398
                     /locus_tag="PK146_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPE90824.1"
                     /translation="MAEQYGSFYPWQVKAWERIRNRDAVLAAPTGSGKTWVAYLWSGL
                     MDLDGHIWPPDTRRIIFTAPIKALSNERYLELRRMGLDVGIETGDFKKNENAPVICCT
                     QEIYTLKYAGSPGLRVVIDEFHYIFDDPDRARTYIEGIVSTNPDSPILVMSATFGGAR
                     SVGDYLGEITGRPFTVYEGQERVTKLVFTPKKPATLERLHDALVFCFSQKGAVDLAYK
                     IARRRPRKGKAHRQRLYELAEILDVRDVHMPLLRGVGVYHGGMLPKEKLLVESAFRER
                     ILDVVCGTNALALGVNLPAEYVIFAQLVRYHDREPISRNEFLQMAGRAGRKGLFETGY
                     VTWLNRSPQESKGFDTGKVFRQLTAAPPERASVTLRPDYGRLLKGRRTISDEAEYIAD
                     FSLPHGDERIVEGELRRGLKFIERSIREMVTPDRRERFREILADIWYGEMEIGENLEM
                     ACLFLENGKADALTAAQVINLFEKSYLKSLLKVKRFSNHLPPGYEITGMRYLDRTVEE
                     IDGTIYGFEEKIQEIEDSSL"
     gene            complement(9494..10261)
                     /locus_tag="PK146_00900"
     CDS             complement(9494..10261)
                     /locus_tag="PK146_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE90825.1"
                     /translation="MAEKVFRRVIQIDEDKCDGCGQCAEACHEGAIQIIEGKAKLVSD
                     SYCDGLGDCIGECPQEAISFIEREAEPYDEEAVKENMGSKKEQETLPCGCPGTMARSL
                     EGILETHCDASSKPQLRSEEAVSSELANWPVQLRLVPENAPYLKGADILLAADCSAAA
                     SPDFHGRFVRGRILLMGCPKLDDADYYREKLARIIRVNDSPSITVVRMEVPCCGGLAQ
                     LAEAAIDEARVDVELRTVILDSRGKLVEDETVKFRFQ"
     gene            complement(10317..10937)
                     /locus_tag="PK146_00905"
     CDS             complement(10317..10937)
                     /locus_tag="PK146_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HPE90826.1"
                     /translation="MTESLKGINYSLRVSARARRVTLRIAPPEGLVVVIPRNFSKKRI
                     PSLIDEKRQWIEKTLSKVAPQAVTASGTLPPKEYFFPSLKKSLGLEGPNAVSRLRSIA
                     DSHLLPRARVLSLKTGLRPASFTFRNQKRIWGSCNSKRKISLNQKLLFLEPPLVDYII
                     LHELCHLEYRDHSSSFWTFLSSFDPHFRDHRREIRSAQWTIPVWAY"
     gene            complement(10995..12257)
                     /locus_tag="PK146_00910"
     CDS             complement(10995..12257)
                     /locus_tag="PK146_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90827.1"
                     /translation="MVSEMFRIRKETLDEINCFLLDPGNPDVQAIHRVVAKYGTPTEI
                     NAKAKEARCLSNLMGRLERMGSPYLENLKWLVAERDRGAFIPVSQYRKKILGDEATGK
                     TFRESTSVTLEISALQYFPFLIAEAEQAIDGKEIMPGRYIRVRKMKEQESDQGDILAV
                     AAAMQIVGASYVETLDTKGTDGSNIHLGGPETITGYFGGIGQPNDHALAWVDELLHYY
                     TNYGINEVLNINPGTVLLGYLLYRLGVDVRFKISVFMGNDNPYAVLWTLMMAKLFARE
                     DGSTPLIGFNLSNSVDAEALMACADIRRRLGLEDKVRIEHHITETYRSIVRQPYNRRE
                     DLLKVARVYPNISAKHEGGDPEVEQTIAHPSDILDYFRDKEEVIASGDMDNLKNNYLK
                     KHDATNRTAEELTRAGLSFLAASRLHAR"
     gene            12336..12500
                     /locus_tag="PK146_00915"
     CDS             12336..12500
                     /locus_tag="PK146_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90828.1"
                     /translation="MKGRRNDLIIFLLIYSTFAYYNLLRSGGGYPGERGTGEGVRGDD
                     PNNGKEPFPS"
     gene            12475..12924
                     /locus_tag="PK146_00920"
     CDS             12475..12924
                     /locus_tag="PK146_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPE90829.1"
                     /translation="MARNLSLLDRDRASCCGVTLAQCHAIVEIGRAEVATPSFLATTL
                     RLDRSTVTRLTDSLVEQGFVVRSQDKVDRRSVTLSLTEKGLRFFEGTENSMNSFFLSL
                     IERIPENHRNVFLSGLGSVVEALERGECVCFDLERLLKGRTEGGCAF"
     gene            12921..13724
                     /gene="arsM"
                     /locus_tag="PK146_00925"
     CDS             12921..13724
                     /gene="arsM"
                     /locus_tag="PK146_00925"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPE90830.1"
                     /translation="MKDIRETVRKKYAEAVRKNRSCCGSGSSCCASGSDPITEGIYQG
                     TSKDRIPDAVLGQSLGCGSPVEMAGLKPGEAVLDLGSGAGLDAFLAGGEVGPSGKVFG
                     LDMTDEMILAAESNRVHWGLDNVEFLKGFIEEIPLPEGSVDAVVSNCVINLSPDKDAV
                     FREIFRVLKPGGRISVSDVVFLRPVPEEIRSSIDAWAGCIAGALGMEEFAGKLKAAGF
                     ENVRVEPTRVYEFTPEQLQDMFPDMQGVADIDAKDVAAGAWISAVKPLS"
     gene            complement(13748..>14130)
                     /locus_tag="PK146_00930"
     CDS             complement(13748..>14130)
                     /locus_tag="PK146_00930"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HPE90831.1"
                     /translation="PITVKSLSELLEGSLSRENEEEIMQAISEVPGVEGAHHLRTRKV
                     GSDVAVEVHIIVARDLGITEAHNIATEVESAIRALHGEGTFVSVHPEPESGDLQGQVP
                     FDDGHNGMEDGPGHHERVPDDLHE"
BASE COUNT         3088 a         3920 c         3922 g         3200 t
ORIGIN      
        1 gaactgcaaa agcaatattt cttttcatgg ctatctctcc tttttttctg gtttgtcctc
       61 ccattcagca atagtctcct ctcagtgaac cgcctccctt tactagtgtc tgaactgcag
      121 attgtacgac agctcttcag ccccttcctt caaggcttgt atccattcat caacattccc
      181 atcggtggca tgggcctttg gtgcggcaag ggcaaggcat gaccataccc tcccatcctt
      241 gtcaaaaaca ggaacggcaa tagacaggat gcccaggctg aaggtttcat cctcaagtgc
      301 gtaacccctt tcgcgtatga tcctgtattc gtccatcagc tggtcaagat cggtcattgt
      361 tttcgaagtt cttttctcca gcttcatgga tccaaggatc tcgcgtatat gcccggggtc
      421 ctggtatgcg cacaggacca tggctgaggc accggagttc aggggaagac tcatgccaat
      481 gaaatcgttg tactcgtaaa taccgtcttt tacgctgttc ttgtaggccg gaaaggccct
      541 gtcgccttcc cagatggtca agtaggtcgt ctcctggaat gacctggaaa tgcggaagag
      601 aaggggcttc gcgatctgtt ctattccctt gaagcggctg tatacgtttc ccagacgtaa
      661 cattatgggg cccaggtgat atttcttcga ggaaggatcc tgaattacga agttgtgttt
      721 cttcatgaca tttatcagct tgaaggcacc acttttaccc attccgacgt gagaggcaag
      781 ttctgaaagg ttgagctcgt atggcgaatc gccaaggcgt ttaaggatca tgagagactt
      841 ttcaagactg ctcatttccc tcctcctttt tcctaatagg aaaaaaggtt tccaaatttt
      901 gcataagtga ttataccagc cagacatgga actgtcaaat caaccccaag aacaatcaca
      961 tagataaagc agtgaattgg attctcctct gttttgaacc ctcctggcag gttagaataa
     1021 gataggtgat aagaccttac aggaggacga ccgatgaaga tagaaccctt tggcgtagag
     1081 cagtggatga atcgctggga gacacttgcc gtcaacaaca tcgcagagac ttgcgtggat
     1141 tcgctgctcc tctacgaact gcttgagatg tcgggccgca aggaggaggt catggtagat
     1201 ctcctgggca cccggatgac ctacggggac ataaagggga gcgagaggct ccggggcctg
     1261 ataggggagc tctacgagga cagggatccg gagagagttt tgataatgaa cggcgggtcg
     1321 gcagccaact tcctggcgct ctttaccatc gtgggtcccg gagaaagggt gatcagcgtc
     1381 tggcccacct accagcagct caccagcatc ccaaaggctt tcggggcaaa agtggatatc
     1441 cttcaactgc ggcccgaaaa tttctggctt ccagataccg atgaactaga gaagatggct
     1501 gaaagcggcg tagacctgat ctgcctgaac aaccccaaca accctacagg gtccctaatg
     1561 gacagggaga cgctggagag gatagcagtg atagcacgta aacacggggc ctgggtactc
     1621 tgcgacgaag cctaccgcgg cctggtccac gaacccggtg tttcggtctc gtcaatggct
     1681 gacatctacg aaaaaggcgt aagcacgggc agcatgtcca aggtcttctc tctggccggt
     1741 ctaaggagcg gatggatgac cggacccaga gagttcatcg aagaggcctt ttctaggagg
     1801 gactacacca ctatcagttg cggacccctt gtggacgccc tggcaaccgt tgcccttgag
     1861 aaccgggaca agatcacgga gaggaacctt aagatcatca gggagagcgt cggcctgctt
     1921 ggggaatgga tcgactcaga acctctcttc cactgggtta agccaacggc tggtacaact
     1981 gcctttgtgg gctacgacct ggatatacca tccgacgagt tctgcgtgga cctgcttggt
     2041 accacgggga catttcttct cccgggtagc tgtttcggga tcgacaagag gctgcgtttc
     2101 ggatacgcct atggaaccga tgcgctgaaa cgggggcttg agtcgctatc ggggtgggtg
     2161 agggagcgtg aatcgtgaga cgtaactcgg aaaacccttt aatcgtgaat cgtaaaccgt
     2221 gtcgcaagtt ctggagatat cgtcatcccg aatccgcctg ctgtcggacg aggcgggacg
     2281 tccgcctatc ggcggagaac tgatccaaca gccccttgtt cagattcctc gtcgttgcac
     2341 tactcgggat tacataaaaa ggttcctacc tcatcacttc gttcggggta cttcgtctgt
     2401 agccctaaac tacgctggag gtgcgcttag ggtacttagt atgtggtaca agatcttttt
     2461 tttcggttca cggttcacga ttcacggttc acggatctga aggcactccc cggaaacgac
     2521 cccctcggcg acggccgtgg tctgcccctt gaggaagaga cgaaccctgc cgggaccctc
     2581 ttcgtcgatg aggacagtat ttacccctcc ggggtttctg acctccaccg gggttgacac
     2641 cttgccctcc agccatgcag cgactgcgcc tgcggttgag ccggtaccac aggacatggt
     2701 gaaatcgttg acgccccgtt cgtaggtccg gaggtcgatc tccccggtgg tcctgatctt
     2761 catgaagttc acgttcgttc cctgggggaa acgctccctg tcggactgga aggccctggc
     2821 gatcctggca agatccattt cagccctttc atcccccggg tcctggaaga tcaccgtatg
     2881 gggaaccccg acattaagct gactgtagac taacctctcg ccggagagta tcagaacctg
     2941 tcccctgagt gccgatgctg tttcgaggac ccccatgtcg agggttgcaa agaccccctc
     3001 cacctctgcc actacgggcc ctgccagggt gtcgatggtc atcctggctg gagccatacc
     3061 cttctcgaag gcgtaacggg ccaggcacct ggcgccgttg ccgcacatct ccccttcgga
     3121 tccgtcgcag ttgaagaggc gcatacggat atcgccccta tcactccttt caacggccat
     3181 cagcccgtcc ccaccgatgg acgtcctcct tcggcagagg cgaagcgcaa gatccgggag
     3241 ttcatggcca ggtatctccc ccaacctgtt gtcgatgatc agaaagtcgt tgccgtttcc
     3301 gtgcatcttg gtgaagagga tcccctctcc cgacgtgttc attcctttat gctccctgtc
     3361 cacagcctgc ttgcagaact ctccggtgag atcataagcg cctcctcgat ccttgaaagc
     3421 ctttcgtcaa ggaccatcgc catcctggcg atatccctct ccaggctcga aacttctgcg
     3481 gcagcttcaa cgctggtacg cccccccgtc tggtggatcc tgcttatgcc gaagagaacg
     3541 tcctctccct tccatctccc gggaacgcca agggtcgttg catgaaccac acttccgtct
     3601 ctttttgcat agggcacatt ggaggtctgc tgctcccggg agagcatcct gcgaaagatc
     3661 ctggccgctt caggcctcgt atcatcggga tgaaggtcaa ggaggtggat cctcccattg
     3721 ttatcaagac cgagctcttc ccgagcggtt ctgttaatcc agactatctc tccgtcaagg
     3781 tcgaaggcaa agaccatctc ggccatggcg tcgaaaaggg atagcagctc ccaggactcc
     3841 ctgatcctgc tctcgctgac ctggaccctg gctatggtct tgtcaaacca ccgggagaga
     3901 gcctcaaggt cacgttttac gtgctgtgga actgtgtcgc tgtcgtgaga ggcgagacag
     3961 aaacaacctg ctactgagga gccccggaag agggggagaa ttgcgaaagc gtggagaccc
     4021 tcggaggcaa ggagcggatc ctggccgttc agggtctcgg aaaaggaccc gtaccatgga
     4081 gtatccctga agatcgccct cccgtgtaca gaatcgggag cccaggaagc gttggcctcc
     4141 acgaaaggat cactgacccc aattgagcaa cggagcctga atatttctgc cttctcgggg
     4201 accaggtaga cccatccaca gtcaagagag ccttccgaag atcctatcaa cgcctcgagg
     4261 cagataggca gagcttcttt cagggtggca gcaccggcca gtgccatggc ccgttcaaaa
     4321 ccgctaacac tttgttgaga tctggtccag tcgctagtca tttgaagtct cttcccttcg
     4381 cgataccaat gaacaaaaaa gcggccacgg tccaagccgc aaggatccga tcctgaccct
     4441 tcaatactcc gaggctagtc actgaaagat aataaacacc aaagacccgg gggatgcaag
     4501 ttcaggaaaa tacggcctga attacacgaa tacagtccct tttgccgtta tctctacaag
     4561 gctgtgggag aaaaaggccc tggttacctc ttcacctttt ttctccacca ccacggtggt
     4621 tatgtcgggg ccgccaagag cagggcctcc accggtctcc agtctgacct tgattcttcc
     4681 gcccttaacc aggccaagat cttttccctc gccggtcaac agaagggcca gggcaagggc
     4741 tgtactgccc gtcccgcagg tgggctcgat gttaccagtg agaaccccgt gggggaagac
     4801 cgccctcaga tgccccttgt gggcgggcga tctgtcaaag agtgaaaaat gaagggtgga
     4861 ggagtaaccc ttctggtgga agaaggcctg cgcctcggat atggccttca cggcggcggt
     4921 gttcatctct tcgaagtcgg cctcggggta gtgttccttc aatttcggag cttcctggac
     4981 aaggtagtag ccggacttga aggcctggat cccgtcgagg tccacaggca tgaccccgtc
     5041 gccggccaac atccgggcaa aggaggtcat gtcgctttcg gcctttctga acttgccgcc
     5101 ttttgtctta tggaccgtca ccgtggaagg acccgactcc agttcaaggg taacctcgaa
     5161 ttcctgtgga gcggtcttca ccttgaacca ctttaccagg tccgtatcca ccagggcctt
     5221 tcccaggacc tgagtgaaac ctccgcatgc agggatgaag ttacggttgg taatatccac
     5281 cacgagagcc cggatggtgt tggcctttcc cgaggggtag agaaaggcgg cctggtcggc
     5341 gtaaagtctc ttccggtcga gaacggtgag ggccgtctca agcacatgag attcccttct
     5401 gacctgcctt ccgttgaaga ggaggatggt gttgcctccc atgtgaccct tgatgaaatc
     5461 gagctccatc gttttcggct cctttcggtc atctcctgtt ccgtaatttt acaggaaaag
     5521 gcaaaacctt aaaggcgtga acggtaaaca gtgaacagtg aacggcaaaa aaggccgaag
     5581 atcgatcctc cggcctttga gcattggttt ctctttctgc ttcactgctt tcgtttaaga
     5641 ggtctcccag gaggagtgcc agggggtacc cctcgttaaa gaacctcccg agtttaatcc
     5701 cctcgggact ttgatccttc cgttcaccgt cgaccgttta ctgtttacgc tttttctccc
     5761 ttgagcgtca cgcccctaaa atctaacgct gtagctgatc atgaagatat gggcatccat
     5821 gttgctggtg cgtgaatcgg gtattctgtc tccgggccgg ccagctatgg tcctgtcgtc
     5881 ggccagcaaa taggtgtagg caaaatccca ggttcggtcg ttcttagtgt agccgaaccc
     5941 tatggagaaa agctgtcggt cgctcatggg agcctgaaag tcgactgtgc cgtccggcga
     6001 aggctcctgg tcgtaggtgt agccgaacct ccaggtccac tcggggctgt acctgtactc
     6061 aagccccagc tggtacctcc agacgttctt ccagttcttc actgaagtgg atgggttggg
     6121 gagcatcccg aaatcgttct cgaaatgtat cgccaacctg tcataggagc tccatccagt
     6181 gtagaccgct cccacctcaa cgttaagttt aggagttacc tgcctgctga ggccgaacag
     6241 gaacatctcg ggcagggtta gatctcccca ggcatccgta gagccgacac cctgtatgtc
     6301 ggcggttccc gagatggtca attttacctg gctcctgtag tgaagcccta tccgggtcgc
     6361 ctcgtccagt ttgtaatgga gtcccacgtt ccagccgtag cctatgtcgt ctccaaggag
     6421 cctgaagtct gttccaataa atcttctcgc cccgtactcg aaccacagcg cctcgacccc
     6481 gacggaaagg gacaaccggt cgttcacctt ccaggccagg ttggggttga tggatacgga
     6541 ctggatgttg gcgtagtagc tgttgtagcg cccgtaccaa tcgggcggga agtcggtgcc
     6601 gagaccgaag cgcgtcatga ggcccacgcc gaaccagagg cggtcactca actgtcgcgt
     6661 gtagtagaag tgaggaggga tgaaggtgtg gtcgatcatg ccaacactat caccgcctat
     6721 ctccgtcagg gtccctcccg gcatgatgaa agccagcccc gccatcatgg acgagtccgg
     6781 gatctgggtg atgcccgccg ggttaaaggc tatggcggag gggtcgtcgg cccgaccggt
     6841 catggcgccc ccgagggcgt tccctctggc actccagtcg taaagagcaa acccggcccc
     6901 cagggctgtt ccgcacccaa gggctgtcat gatgatcgcc gaaataagaa taagaacagg
     6961 tcttttcaag aagcactcct ccttttttat cctgtcaggt gtcgcatggt cacgggaaca
     7021 gccctaatat tggaagcctt ccagcttgtc gtcacccatt actcctctga caagctcctc
     7081 catagccctt tcgatgcgcc cccacccctc ttcggatgag atgtctatcc ccaggtaggc
     7141 ctccgatgtc cgtgccctga gcatcagaaa ggtgagaccc gccgagagaa gggcacccag
     7201 ggcggcaaca tccaccctct ccgcttcagg ggcggacctc accagggcct ctataaggtc
     7261 cgctccctgc ctctctctct tccgtgccga tgcctcggag agttcgttcc ttgataccag
     7321 ttccccaagg agcagttcct gggctgatgt cgaatccctt agctcccgaa tgtagccctt
     7381 gagaagggcc acggcgagtt cggaagctcc gggaggccgg ttatcccctt ccgccttttc
     7441 gaccagatcc tccatcgagg gccagtaatc gccccctgaa gcgaactccc tcagaagccc
     7501 tggaagatct ccgaagtagc ggtagatcag gaccttgtcg acgccggctc tccgggatac
     7561 gttgttgacc ccgatgcctc cgaacccctc ttctgaaagg atctcgccca ccgcttcaag
     7621 gatctttttc ctggtctcct ctttatcgcg catggcacct cctgtcacca accggtgaca
     7681 taaaccgatg atattatcgg atttctcctc ttgtcaagga agaggcaata ttgaattcgt
     7741 aaaagggacg ttcatagtaa tataagtagc gccgggacac ttgcgacaac acttcacgaa
     7801 atgaggcgga catggcggaa caatacggaa gtttctaccc ctggcaggta aaggcctggg
     7861 aaaggatcag gaacagggac gccgtcctgg ccgcgccaac cgggtcgggc aagacctggg
     7921 tggcctacct gtggtcgggc ctaatggacc ttgatggtca tatctggccg ccggacacga
     7981 ggcggatcat cttcaccgcc cccataaagg ccctgagcaa cgagcgctac cttgaactca
     8041 ggcggatggg gcttgatgtg ggcatagaga cgggcgactt caagaagaac gagaacgcac
     8101 cggtcatctg ctgcacccag gagatctaca ccctcaagta cgccggctcg ccgggattga
     8161 gggtcgtgat cgacgagttc cactacatct tcgacgaccc cgaccgtgcc cggacctata
     8221 tcgagggaat tgtctccaca aaccccgact cgcctatact ggtcatgtcg gccactttcg
     8281 ggggcgcccg ctctgtcggc gattacctcg gagagataac cggaagacca ttcacggtct
     8341 acgaggggca agaacgtgtg acaaaactgg tattcacacc gaagaagccc gccacccttg
     8401 aaagacttca tgacgccctg gtcttctgct tctcccagaa aggagctgtg gacctggcct
     8461 acaagatagc ccgcagacgc ccccggaagg gaaaggccca caggcagcgg ctctatgaac
     8521 tggccgagat ccttgatgta cgggatgtcc acatgcctct tctgaggggc gttggagtct
     8581 accacggagg gatgttgccg aaggagaagc tcctggtgga gtccgccttc agggaaagga
     8641 tcctggacgt ggtctgcggt acgaacgcac ttgccctggg ggtgaacctc cctgcagagt
     8701 acgtcatctt tgcccagctc gtcaggtacc acgacaggga gcccatatcc aggaacgagt
     8761 ttctgcagat ggcgggaagg gccggtcgta aaggcctctt cgaaacaggt tacgtgacct
     8821 ggctcaaccg ttccccccag gaatccaagg gctttgatac cgggaaggtc ttccggcagc
     8881 tgacagcggc ccctccggaa agagcctcgg taacactcag accggattat ggaagactgc
     8941 tgaagggacg gagaacgatc agtgacgagg ctgagtacat agccgacttc tcactgcccc
     9001 acggggatga acgtatcgtg gagggtgagc tcagaagggg cctaaagttc atagagagat
     9061 ctatccgcga gatggtgacc cccgaccgga gggaacgatt cagggagatc ctggccgaca
     9121 tctggtatgg agagatggag atcggcgaga acctcgagat ggcctgtctc tttctggaga
     9181 acggaaaggc cgatgccctt acggccgccc aggtgataaa tctcttcgag aagagctacc
     9241 tcaagtcgct actcaaggtc aagcgcttct ccaaccatct ccccccgggg tacgagatca
     9301 cggggatgcg ctacctggac cgtaccgtgg aagaaatcga cggaacgatc tacggcttcg
     9361 aggagaagat ccaggagata gaggacagtt cgctgtaagg cctgcagcca gtaaggcgaa
     9421 caccgggtaa tttccttgca gcttcccgta ggcctgctgg cctcaagtct atgggggttt
     9481 acaatacccc taattactgg aaccggaact tgactgtctc gtcttcaacc agtttcccac
     9541 gggaatcaag aatcacggtt ctcaattcaa catccaccct tgcctcgtca atggccgcct
     9601 cagcaagctg tgcaagacct ccgcagcagg ggacctccat cctgacgact gttatcgagg
     9661 gcgaatcgtt gaccctgatg atcctcgcaa gtttttccct gtagtagtct gcgtcatcaa
     9721 gtttgggaca ccccatgaga aggatgcggc ctctgacgaa acggccatga aaatccggag
     9781 aagcagctgc cgagcagtcc gctgccaaca gaatatccgc acccttcaga taaggggcgt
     9841 tctcaggtac aagtctcagc tggacgggcc agttagccag ttccgaggag acagcctctt
     9901 ccgatcggag ctgaggcttg cttgaagcat cgcaatgagt ttcaaggatc ccttccaggg
     9961 atctagccat ggtgccaggg catccgcagg gtagagtctc ctgttctttc ttcgaaccca
    10021 tgttctcctt gacggcctcc tcatcgtagg gctctgcctc acgctcaatg aaggatatgg
    10081 cttcctgggg gcactcccct atgcagtctc ccaacccgtc gcagtaggag tcgctaacaa
    10141 gctttgcctt gccttctatg atctggatgg ccccttcgtg acaggcttct gcacactggc
    10201 cgcagccatc gcatttgtct tcgtcgatct ggatgaccct tctgaaaacc ttctccgcca
    10261 tagtgaacac ctcacagttt attttaggta ttatggtacc aaaataagaa actgtgtcaa
    10321 taggcccaga cggggatcgt ccactgtgcg gaccttatct ctctccggtg atccctgaag
    10381 tgtggatcga aggatgaaag aaaggtccag aatgaggacg agtggtctct gtactcaagg
    10441 tggcagagct cgtggaggat gatgtagtcc accaggggag gttcaagaaa gagtagtttc
    10501 tggttcaggc tgatttttct cttcgaattg cagctccccc atatcctttt ctggttcctg
    10561 aaagtgaagg aagcgggcct aagtcctgtc ttaagcgaca aaacccttgc ccgagggagc
    10621 agatgggagt cagctataga gcgaaggcgg gaaacggcgt tcgggccctc gaggcccaaa
    10681 gacttcttca gggaggggaa aaagtactct ttcggaggca aagtcccgct tgcagtcact
    10741 gcctggggcg ccacctttga aagggtcttt tcgatccatt ggcgcttctc atcgatcagg
    10801 gagggtatcc tcttcttgct gaagttacgg ggtattacca caacaagtcc ttcaggggga
    10861 gcaatccgta aagtcacacg gcgtgccctg gcgcttaccc ttaaagaata atttatccct
    10921 ttgaggcttt cagtcatggt ccatccgata gaaaagttgc ccgggaagat cttcccgggc
    10981 aaccaggatc atgatcagcg tgcatggagg cgagaagcgg ctagaaacga gagccccgcc
    11041 ctcgtcaatt cttctgcggt tctgttcgta gcatcgtgct ttttgaggta gttgttcttc
    11101 aggttatcca tatcgccgga ggcgatgacc tcttccttgt cgcggaagta gtccagtatg
    11161 tcagaggggt gggcgatggt ctgttctacc tccgggtcgc ctccctcgtg ctttgcggaa
    11221 atgttcgggt agacccgggc gactttcaga agatcctcgc gcctgttgta gggctgacgg
    11281 acgatgctcc tgtaggtctc ggtaatgtgg tgctctatcc tgaccttgtc ctccaggcca
    11341 agtcttctgc ggatgtcggc acaggccata agagcctctg cgtccaccga gttggagagg
    11401 ttgaacccga tcaacggcgt cgacccgtcc tccctggcga acaatttggc catcatcagc
    11461 gtccagagga ccgcgtaggg gttgtcgttg cccatgaata cggatatctt gaagcggaca
    11521 tccaccccca gcctgtagag caggtaaccc aggagtaccg tcccggggtt gatgttcaga
    11581 acctcgttga tcccgtagtt cgtgtagtag tgaaggagct cgtcgaccca tgccagggca
    11641 tggtcgttgg gctgaccaat gcctccgaag tatcccgtga tggtctcggg gccacctaga
    11701 tgaatattgg agccgtcggt ccccttggtg tcgagggtct ccacgtagct ggctccgacg
    11761 atctgcatgg ccgccgccac ggcaaggata tcgccctggt cgctctcctg ctccttcatc
    11821 ttgcggaccc ttatgtatct ccctggcatg atctccttgc cgtcgatggc ctgttccgcc
    11881 tctgcgatca ggaaggggaa gtactgcagg gcactgatct cgagggtcac tgaagtcgac
    11941 tctctgaagg tctttcccgt ggcttcgtcg ccaaggatct tcttccggta ttgcgagaca
    12001 ggaatgaagg cacccctgtc cctctccgcc acaagccact tcaggttctc caggtaagga
    12061 gatcccattc gctccagccg tcccatgagg ttcgaaaggc accttgcctc cttagccttc
    12121 gcattgatct ccgtcggtgt accgtacttg gcaactaccc tatgaatggc ctgtacgtcg
    12181 ggattgcctg gatcgagcag gaaacagttg atctcgtcca gtgtctcctt cctgatacgg
    12241 aacatttcac tgaccacaac tatcccctcc ggttcatgac tttggaatgt tctttccaga
    12301 aaacaaaacc ttgatcttta aaaatcatac catgcatgaa gggaagaaga aacgatttaa
    12361 ttatattttt attgatttat tcaacttttg catattacaa tcttttgcgt tcaggaggtg
    12421 gataccctgg agaaagagga acaggcgaag gcgttagggg agatgatccg aacaatggca
    12481 aggaaccttt cccttcttga cagggacagg gcatcatgct gtggtgtaac ccttgcccag
    12541 tgccatgcta ttgtcgagat agggagagcg gaggtagcga ccccaagctt tctggcaaca
    12601 accctgagac ttgacaggag cacagtaaca agattgacgg acagccttgt ggagcaggga
    12661 tttgttgtca ggtcacagga caaggtagat cgaaggtccg taacactttc gttaacggag
    12721 aaagggctcc gtttttttga agggacggag aattcaatga acagtttctt cctttcattg
    12781 atcgaaagga tcccggagaa tcatcgcaat gtcttcctct cgggtctggg atccgttgtc
    12841 gaggccctgg agagaggcga gtgtgtctgt tttgatcttg aaaggctcct gaaggggcga
    12901 acggaagggg gatgcgcatt ttgaaggaca tacgcgagac tgtaagaaag aagtacgccg
    12961 aagccgtaag aaaaaacaga tcttgctgcg gttcggggtc atcttgctgc gcttcaggat
    13021 cggaccccat tactgaaggc atctatcagg gaacttcaaa ggaccggata cccgatgccg
    13081 tccttggtca gtccctgggt tgcgggagcc ccgttgagat ggccgggctc aagccggggg
    13141 aagcggtgct ggacctgggg agcggggcag gcctcgacgc attcctggcg gggggggagg
    13201 taggtccttc cggaaaggtc ttcgggctgg atatgaccga cgagatgatc cttgcggctg
    13261 aatcgaacag ggtccactgg ggccttgaca acgtggagtt cctgaagggc ttcatcgagg
    13321 agatccccct tccggaggga agcgtggatg cggtagtctc taattgcgtg ataaatttgt
    13381 cccccgacaa ggatgctgtt ttccgggaga tcttcagggt gctgaagccg ggcggcagga
    13441 tctccgtttc tgatgtggta ttcctgaggc cggtgcctga agagatcagg agcagcatag
    13501 acgcatgggc tggatgcatc gccggggcgc tgggaatgga agaatttgca ggaaaactga
    13561 aggcagcggg gttcgaaaat gtcagggtag agcctacaag ggtttacgaa ttcactccgg
    13621 agcagctcca ggacatgttc cccgatatgc agggggtagc tgacattgac gcaaaagacg
    13681 ttgctgcagg agcctggatc tcggctgtaa aacccctttc ctgaagtgga ggtcggtctg
    13741 agaggcatca ttcgtggaga tcatcgggaa ccctctcgtg gtgacccggg ccatcctcca
    13801 tgccattatg accgtcgtcg aaaggaacct gaccctggag gtctcccgac tcaggctcgg
    13861 ggtgcacgga tacaaaggtc ccttctccgt ggagcgccct gatggcggat tcgacctccg
    13921 tggctatgtt gtgggcctcg gtgatgccga ggtccctcgc cactatgatg tgaacctcca
    13981 cagccacgtc agacccgacc ttcctggtcc ggagatggtg tgcaccctcg acccccggga
    14041 cttccgatat tgcctgcatg atctcttcct cgttttccct ggagagggac ccttcaagca
    14101 gttcgctaag gctctttact gtgatgggga
//
LOCUS       DAOPKQ010000012        13107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_4986_length_13107_cov_4.802712, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000012 DAOPKQ010000000
VERSION     DAOPKQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13107
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4986_length_13107_cov_4.802712"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK146_00935"
     CDS             complement(<1..188)
                     /locus_tag="PK146_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90832.1"
                     /translation="MRGEVGVFKLDLFWIFILFFFVFPMFKQWNLERMRVAAIRKLEK
                     KRSSRVITLIHRQESVGFL"
     gene            complement(247..2772)
                     /locus_tag="PK146_00940"
     CDS             complement(247..2772)
                     /locus_tag="PK146_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPE90833.1"
                     /translation="MWQYFTERGKKVIQLAHREALRMGHDVIGTEHILMGLAAEGEGV
                     AAQILQSSGVSLDELRSTVEQFVGRGEQKSKPVDLPLSPRAKKVLDLAMREARGMSVN
                     YVGTEHILLGLISEGEGIAAQILGSIGMDLEKIRTEVIAAISAGEGSTAEAAESPGED
                     HPQKSAGRSRTPTLDQLGIALTEMAQRNELDPVIGRAKEIQRLIQILSRRTKNNPVLI
                     GDPGVGKTAIVEGLAQKIIAGDIPEVLRGKRVVQLNVGNLVAGTKYRGEFEERMRKLV
                     KELRDCRDVILFIDEIHTIVGAGGAEGAVDAANILKPSLSRGEFQVVGATTVDEYRKN
                     IEKDAALERRFQPVMVEEPGVEDSIRILEGLRDRYEAHHRAKITDRALDAAARLSARY
                     ITERHLPDKAIDLIDEAAARARLKTMEAPEDIKEKERLLDALRKEKEAAVASQEFEKA
                     ASLRDDERKLGDEIEVCRKEWQKVRNQEEPVVDAEQIAGIVSEWTGIPVVQLTEEEAA
                     RLMRMEEEIHRRMVDQTQAVNAVSRAIRRSRSGLKDPKRPVGSFLFLGPTGVGKTELA
                     RSLAEFLFGSEEAMVRFDMSEYMERHEVAKLIGAPPGYVGFEEGGKLTEALRRRPYSV
                     VLFDEIEKAHPDIFNILLQILEDGRLTDGQGHTVDFRNSVVIMTSNIGAKDAMKGQGL
                     GFTAPGQGEMPDWGRVRTGIVDSVKKTFRPEFINRIDEMIVFEPLGREDLLKVLDLML
                     REVSERLSEKGIHIEIPVEARTLLLDKGYDPKYGARPLRRAVQRLIEDRLADLVLEGR
                     VADESLISVSVEDGELVLVPDAMPLEGGDDVREGMEAEEKADG"
     gene            complement(2823..3890)
                     /locus_tag="PK146_00945"
     CDS             complement(2823..3890)
                     /locus_tag="PK146_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HPE90834.1"
                     /translation="MKAGAREIADSPLEWVGGKGEHSGMILSSRVRLARNLKGHVFPW
                     RADKKELSVCSERVRSSIRSEDSLKPFSIFEVDALDPLSRGVLIEKHLISPALAMGGP
                     GRGAAVDGRGIVSIMINEEDHVRIQAILGGMSIHEAWRIARCMDELLEKLDYAFHSRY
                     GFLTACPTNVGTGLRASVMIHLPALTHLRRMGKLAVDCGKIGLTVRGMFGEGSDSPGS
                     LYQISNQVTLGQTEEELIEKVSGAALGLVRQEEGARDRILSERGPGLEDKVWRAWGLL
                     RFSRVIEAREALELLSLVRMGSEMGILPQVRVATLNALLLDIQKAHIQARSATELSPE
                     EIGRKRSEVLRKALATTGGES"
     gene            complement(3874..4410)
                     /locus_tag="PK146_00950"
     CDS             complement(3874..4410)
                     /locus_tag="PK146_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPE90835.1"
                     /translation="MKCERCGAREAEVHIRQQKGKEIAEFHLCRQCAEHMAKEGLIPD
                     ISFELPIDSFLWGLLPGHGPSHPGPDMAPSTTGDMVCGSCGLDRSSLRRSGRYGCPDC
                     FKAFHEDLGPLLRKIHGSEIHRGARPGNACGNDTSPEEIESLRTELKDAVEKEEYERA
                     AVLRDRIREIGDLHEGRS"
     gene            complement(4422..4901)
                     /locus_tag="PK146_00955"
     CDS             complement(4422..4901)
                     /locus_tag="PK146_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HPE90836.1"
                     /translation="MASLTRIIEKHIEELLERASEESVFLSRKDLAERFGCVPSQINY
                     VIRSRFTPERGYIVESQRGGHGYIRVIRLCLDMPEARANHIEELVGDTVNEQDIRRIL
                     ANLQHRQLITARERLLIEVAARFQEEIGRDLLDLSPYKRDSLHAELLKRLLRSLALG"
     gene            complement(4991..5578)
                     /gene="plsY"
                     /locus_tag="PK146_00960"
     CDS             complement(4991..5578)
                     /gene="plsY"
                     /locus_tag="PK146_00960"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPE90837.1"
                     /translation="MTAFIWVLAGYFFGSIPAGYLIIKILLGKDIRNLGSGNIGATNV
                     SRFVGKGWGVGVALFDMAKGGLVVLAARLAGIQDPWILALSGFAGVCGHNFPLWLRFK
                     GGKGVATSFGVVFFWNPVPAAAGGIVWYLAMRLSRYVSVGSMVSLATVPVWFLLLRES
                     SAYVWTSAAMALLSVVRHRSNIINLSRGEEGRTGS"
     gene            complement(5575..7689)
                     /locus_tag="PK146_00965"
     CDS             complement(5575..7689)
                     /locus_tag="PK146_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPE90838.1"
                     /translation="MACPAARSGDLSKLLSPRSVAVVGASGNLESISGRPVKLLKRFG
                     FKGRVFPVNPNRKVVAGLECFPDVRSLPETPDVVLVGVKAVLVPEVIGQCSERGVPYS
                     IIFSSGFAESNDPAAQDDILEKARSGGMRILGPNCQGLVNFADSVPLSFSASLDSDRR
                     PLGSVAYLSQSGAFGFASFAMAAESGVGFRYVVTTGNQADLDLVDFGMVFAADPEVRL
                     LVLYMEGLSDGARFIEMLRFAGERNVPVAVLKVGKSPTGQAAVKSHTAVLTGEDEAWR
                     TIFRQYGVIEIEDSDDIVDLGKIFGSGKRPKGKRLAVVTTSGGAGIIMSDRAFDLKLQ
                     VDPLPPRTRGLLEQFVPPFGSTRNPVDLTAQVINDPGNFRSSMETVLGCPDVDMAAVV
                     LSMITGDSGSAVARDLIEVSRKAVKPLACCWLINSEQGGKFLEQIRDEKIPLYGSPKR
                     CAWALARLAEWTTRPALPARAPSDLGEPFMPKMPADLTEYDAKRLLARWGIPVTREIL
                     CASLEEALSAAGEIGYPVALKVVSPDIIHKTEAGAVALRLKDEEELRNAYGRLLERSI
                     KARPGARIRGVLVQEMVTGGVECMIGVKRDPVFGPLVAVGLGGIYVEVLKDLSLRHAP
                     VDRAEARGMISSLRGFPLLSGARGRTPKDIDTLAAMVSDISLMALAERSLAELDVNPV
                     FVLDRGEGAVAVDAVVIRGSGS"
     gene            complement(7691..8461)
                     /locus_tag="PK146_00970"
     CDS             complement(7691..8461)
                     /locus_tag="PK146_00970"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPE90839.1"
                     /translation="MRIVRFAINEGNDVHWGSLEDERVFVTSSPGGEKTGGSAPLASV
                     RLLAPALPTKIVCVGRNYHGHIREMGNDDLPLPEEPGLFLKGPNSLAAPFSEIPYPHF
                     TNDFHYEGELAVVIGKRILPGTEHPIDHVLGYTAGIDLTARDCQRRDLQWVRGKSADA
                     FCPLGPWIETGINPDRTVVRTFVNGVLRQEGRTEDMIFPISVILKYISEFMTLEAGDV
                     ILTGTPEGVGEIFSGDRVEVSIDGLGSPLVMTVSGEGA"
     gene            complement(8508..8966)
                     /locus_tag="PK146_00975"
     CDS             complement(8508..8966)
                     /locus_tag="PK146_00975"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE90840.1"
                     /translation="MEEMPHQGGGMSLGSLMAEMAFATAGTAAVHALAYPLGGEFNIP
                     HGEANTVMLKSVMEFDRDRCAKKTSRLARAVSMERLNMSDEEASEYLTEWMTGTARRI
                     GVTIRIRDLGVPEDAIPTMADAASREARLLSNNPKTINREDIDKIYRRAW"
     gene            complement(9390..9710)
                     /locus_tag="PK146_00980"
     CDS             complement(9390..9710)
                     /locus_tag="PK146_00980"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE90841.1"
                     /translation="MGVTEACTGDRFSFRFPGRVSFGDGTVSDLPALLSEMKAEHSLI
                     LSGRTISEGPLMERVREILDLSGIRYGVFNGVEPETLVEIVKAAAEAARGAGYDIMTG
                     QVTI"
     gene            complement(9833..11041)
                     /locus_tag="PK146_00985"
     CDS             complement(9833..11041)
                     /locus_tag="PK146_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE90842.1"
                     /translation="MSQEVWEGLFSEVGINLTPSPIRELVKYMKRPGMISLAGGNPDP
                     EIFPVKEFQEAAGVISERGKEILQYGGTGGYEPLKEFISGWMAPRMGRATAPEEMCIT
                     TGSQQVMDLFAAVMLDPGDVVIVEEPTYPGATHALKNRKARFVTVPCDSEGMKVDMLP
                     EILEQARKGRDKVKFIYSIVNFQNPSGYTLSASRRKKLLEIAETFGVPVLEDDPYGHL
                     RFDGEHEPTIFSMDRSGLVIYACSFSKILAPGTRVAWVVGPEEVIGKMIMVKQGSDLC
                     TSVLAQGLVYEYCRLGHMDLFLPRIQEHYKKKRDAMDAAFREYLPPDTTYGTPQGGFF
                     YWLTLPGVNTRDLFFKAIERNVSFVTGDAFFPTGGGEEHLRACFSYANADDFPEAAKR
                     LALAINDLRL"
     gene            complement(11116..11751)
                     /locus_tag="PK146_00990"
     CDS             complement(11116..11751)
                     /locus_tag="PK146_00990"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPE90843.1"
                     /translation="MFSTRRRKWSLPGAFVVVMILSALGLGGYLLLERLSPARTRAIQ
                     AAEDQRIAPAEQDQHPAPDADRIEVFVAENGLEESFWILIGKGCFELSTWNGTEREAV
                     FPIAVGENPGNKERVGDRRTPEGVFRVERTHDSRTWVHDFGDGKGPVEGAYGPFFIRL
                     ETGWKGIGIHGTHDPSSLGKMVTEGCIRMSNEAVMEIVERSVPGTVVIIEP"
     gene            complement(11819..12139)
                     /locus_tag="PK146_00995"
     CDS             complement(11819..12139)
                     /locus_tag="PK146_00995"
                     /inference="COORDINATES: protein motif:HMM:NF040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HPE90844.1"
                     /translation="MGDIRKNPSEDDKKQVTERLAEVIERSRVMANLEILLDRRKLIW
                     NNFLAGLAKGVGFGVGLTVLAAVVIYFLVIVLKSILALNIPVIGNYIADLVQLVQDQI
                     QNQR"
     gene            complement(12148..>13107)
                     /locus_tag="PK146_01000"
     CDS             complement(12148..>13107)
                     /locus_tag="PK146_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPE90845.1"
                     /translation="HIHTDTPDRLFFTIKKHGALTQQKADDMRRQVDVCRKRMHSVAL
                     VTDSTCDLPQEFLDRNQIHVVPLRLAFGESVFIDRVTISSEQFYTLLEESGERPVSSQ
                     PSISDFERTYRFLLEHYNSVIAVHISSKLSGTWNASRAAAEKVGGMITVIDSRTASAP
                     LGLLVMRAAQALNEGKGHEETVSLIEAGIPGAKIFVSLKTLKYMVRGGRVSPAKGLLA
                     TLLNLKPIITVNEEGFARSLGQTRGWEANVNKIREIIDQECRRARVWNYCVVHAHSPA
                     SAEAAASGMREIVGSDPAYVMDISPVLGAHSGIGSVAVGILVE"
BASE COUNT         2657 a         4124 c         3347 g         2979 t
ORIGIN      
        1 aggaagccca cgctctcctg tctgtggatc agagtgatca ccctgctgct ccgcttcttt
       61 tcgagtttcc tgatcgcagc caccctcatc cgctccaggt tccactgttt gaacatgggg
      121 aagacgaaga agaacagaat gaagatccag aaaagatcca atttgaacac ccccacctct
      181 cctctcatta tgaaagaacg gggtcccgga caagggaccc ctgttcccgt aattcattcc
      241 cttgtatcat ccatcggcct tctcttccgc ttccattcct tccctgacat catctccacc
      301 ttcgaggggc atcgcatccg ggaccagtac cagttcccca tcctccaccg acaccgaaat
      361 gaggctttca tcggcgacac gaccttcaag cacaaggtct gccagccggt cctctatcag
      421 gcgctggaca gccctcctca gtggtctggc cccatatttg gggtcgtacc ccttgtcaag
      481 gagaagcgtc ctcgcttcga cagggatctc tatatggata cccttttcgg agagcctttc
      541 agaaacctcc ctgagcatga ggtccaggac tttcaggaga tcttcccttc ccaggggttc
      601 gaagacgatc atctcgtcga tgcggttgat gaactcgggt ctgaaggtct ttttgaccga
      661 atcaacgatc ccagtacgga ccctgcccca gtcaggcatc tctccctgtc cgggagcggt
      721 aaagccgaga ccctgaccct tcatggcatc cttggcaccg atgttgctgg tcatgatgac
      781 cacgctgttc ctgaaatcga cggtatgtcc ctgaccgtcg gtaagacgtc cgtcctcaag
      841 tatctggagg aggatgttga aaatatcggg gtgggccttc tcgatctcgt cgaagagcac
      901 caccgaatag ggcctcctcc ggagagcctc tgtaagcttc ccgccctcct cgaatcctac
      961 atagcctggc ggggcgccga tcagtttggc cacctcgtgg cgctccatgt actcgctcat
     1021 gtcgaaccga accatggcct cttcgcttcc gaagaggaac tccgcaaggg aacgggcaag
     1081 ctcagtcttt cccaccccgg tggggcccag gaagaggaag cttccaacgg gccttttggg
     1141 gtccttgaga ccgctcctgg atcttcttat ggcccgggat acggcattta cggcctgggt
     1201 ctggtcaacc atgcgccgat ggatctcctc ctccatgcgc atgagccggg cagcctcctc
     1261 ctcggtcagc tgaacgactg ggatccctgt ccactcggag actatcccgg ctatctgttc
     1321 agcatctacc accggttcct cctggttccg gaccttctgc cactctttcc ggcagacctc
     1381 gatctcgtca ccgagctttc tctcatcatc cctgagggag gcggccttct cgaactcctg
     1441 ggacgccaca gcggcttcct tctccttgcg gagagcatca aggagtctct ccttctcctt
     1501 gatgtcttcc ggggcctcca tggtcttcag ccttgcccgg gctgcagctt cgtcgataag
     1561 gtcgatggcc ttgtccggca gatggcgttc agtaatatac ctggcagaga gccttgcggc
     1621 agcatcaagg gccctgtcgg tgatcttggc cctgtgatgg gcctcatacc tgtccctgag
     1681 gccttccagg atccttatgg agtcctccac accgggctct tccaccatta ccggctggaa
     1741 gcgtctctcc agtgctgcgt ccttctctat gttcttcctg tattcatcga ccgtcgtcgc
     1801 cccgaccacc tggaactcgc ctcttgacag actgggcttc aggatattcg ccgcatcgac
     1861 agccccttcc gctcctccgg ccccgacgat ggtgtggatc tcgtcgataa agaggatcac
     1921 gtcccggcaa tccctgagct ctttgaccag tttgcgcatc ctctcctcga actcgcccct
     1981 gtacttggtt ccggcaacca ggttgcccac gttgagctgc acgaccctct tgcctctcag
     2041 tacctcgggg atgtcaccgg caattatctt ctgggccagg ccctccacga tggcagtctt
     2101 gccgacaccg ggatcaccta taagcacggg gttgttcttg gtacggcggg agaggatctg
     2161 tatcagtctc tggatctcct tggcccggcc tataaccgga tcgagctcat tgcgctgggc
     2221 catctcggtc agtgctatac cgagctggtc cagcgtcggg gtcctggacc ggccggcaga
     2281 tttctgagga tgatcctcgc cagggctttc agcagcctcc gccgtgcttc cttctcctgc
     2341 cgaaatagct gctatgacct cagtacggat cttctcgagg tccataccaa tggaacccag
     2401 tatctgggct gctataccct caccctctga gatcagtccc agaagaatgt gctctgtccc
     2461 gacgtagttg acggacattc cccttgcttc ccgcattgcc aggtcaagga ccttcttcgc
     2521 ccttgggctc agtgggaggt ccacaggctt gctcttctgc tctccccgtc cgacgaactg
     2581 ctccacagtg ctccgcagtt catccaggga cacaccggaa gactgcagga tctgagcggc
     2641 aacgccctct ccctcggcag ccagccccat aaggatatgt tctgtaccta taacgtcatg
     2701 gcccatcctg agagcttccc tgtgggccag ctggatcact ttctttcctc tttcggtgaa
     2761 gtactgccac atgttctgaa cctccatggt ttattgaagg aatgacctga acgaccccgc
     2821 gatcagcttt cgcctccagt agttgccaga gccttcctga gtacttccga tctcttcctg
     2881 ccgatctcct ccggcgatag ttcagtggct gaccttgcct gaatatgagc tttctggata
     2941 tccaggagca gtgcgttcag ggttgccacc ctgacttggg gaagaatgcc catctcggaa
     3001 cccatcctta ccagggagag aagctccagg gcttccctgg cctcgatcac ccttgaaaat
     3061 ctcagaagac cccaggctct ccagaccttg tcctccaggc ccgggcccct ttcggagagg
     3121 atccggtccc tggctccctc ctcctgacgt accagtccga gggcagcccc ggagaccttc
     3181 tcgatgagtt cttcttcggt ctgaccaagg gtgacctggt tggagatctg gtagagcgac
     3241 cccggtgaat cactcccctc accgaacatc cccctgacgg taaggcctat cttgccgcag
     3301 tctacggcca gcttgcccat tctccggaga tgggtcaggg caggaaggtg gatcattacc
     3361 gaggccctga gccccgtccc cacgttggtc ggacaggccg tcaggaagcc gtatctgctg
     3421 tggaaggcat aatcgagttt ctccaggagc tcgtccatgc acctggcgat cctccaggcc
     3481 tcatggatgc tcataccccc gaggatggcc tggatacgga cgtgatcctc ctcgttgatc
     3541 atgatggaga cgatcccccg tccgtccacg gcagcccctc tgccgggacc tcccattgca
     3601 agggcagggc tgatcaggtg tttctctatg agaacacccc tggaaagcgg gtcaagggca
     3661 tcgacctcga atatgctgaa aggcttgagg gagtcctctg aacgaatgga agatcgcacc
     3721 ctctcggaac acaccgatag ttccttttta tcagccctcc atgggaatac gtgccccttt
     3781 aggttcctgg caagcctgac gcggctggac aggatcatgc ccgagtgctc gcccttcccc
     3841 cctacccact ccaggggact gtcggcgatc tccctagctc ctgccttcat gaagatctcc
     3901 tatctcccgg atcctgtccc ggagcactgc ggccctttca tactcctctt tctccaccgc
     3961 atctttgagt tctgtcctga gagactcgat ctcttcggga gaagtatcat tcccacatgc
     4021 gttcccgggt cttgctcctc tatggatctc cgatccgtgg atctttctga gaaggggacc
     4081 gagatcttca tggaacgcct tgaaacagtc ggggcatccg tacctgccgg atctcctcag
     4141 ggatgagcgg tccagtccgc aactgccgca aaccatatca ccagtcgttg atggggccat
     4201 atcgggtcca gggtggcttg gcccatggcc ggggagaagc ccccagagga agctgtctat
     4261 gggcaactcg aaggatatat ccgggatcag accctccttt gccatatgct ctgcgcactg
     4321 gcggcaaagg tggaattccg ctatctcctt gcccttttgt tgcctgatgt ggacttcagc
     4381 ttccctggct ccgcaccttt cgcatttcat ggcggctcga cctatcccag agccaggctg
     4441 cgcaacaacc gcttgagaag ctccgcgtgg agagaatccc tcttgtaggg tgaaagatcc
     4501 agcagatccc tgccgatctc ttcctgaaac cttgctgcaa cctctatcag gagcctttcc
     4561 ctggcagtga tcagttgcct gtgctgaagg ttggcaagga tcctcctgat atcctgttca
     4621 ttaacggtgt cgcccacaag ttcctcaatg tggttcgctc ttgcctctgg catgtccagg
     4681 caaagcctga taacccggat atacccatgc cctccccgct gactttcaac aatgtatccc
     4741 cgttcagggg tgaagcggct tctgatgacg tagttgatct ggctcggcac acaaccgaaa
     4801 cgttccgcaa gatccttgcg gctgagaaag acagattcct cgctggcccg ttcaaggagt
     4861 tcctcgatat gtttttcgat tatccttgta agacttgcca agacacttcc ccctttgacc
     4921 ttctttgaca agtcaaatta taaagttaag gtcaatattc gtcaataata aggtccaggg
     4981 caaggtcctg tcaggatcct gttcgccctt cctctcctct tgaaaggttg atgatgttcg
     5041 aacggtgacg taccacggaa agcaaggcca tagccgccga ggtccagacg taggccgagg
     5101 actccctgag cagaaggaac cataccggga ccgttgccag ggataccatg gagcctactg
     5161 aaacatacct ggaaagcctc atggccaggt accacacgat gccacctgct gctgcaggga
     5221 ccgggttcca gaaaaaaacc actccgaaag atgtggcgac ccccttgccg cctttgaacc
     5281 tgagccagag agggaaattg tgaccgcaaa ctcccgcgaa acctgaaaga gcaagtatcc
     5341 agggatcctg gatccctgcc aggcgggcag ccaggaccac aagcccaccc ttcgccatgt
     5401 cgaaaagcgc gacccccaca ccccatccct tgcccacaaa acgggagacg ttggtcgccc
     5461 cgatgttgcc cgaacccagg ttccggatat cctttccgag aagtatcttg atgatcaggt
     5521 agccggccgg gatggaaccg aagaaatagc cggcaagtac ccaaataaaa gccgtcatga
     5581 tcctgaacct cttatgacca ctgcgtccac cgcgacagcc ccttcccctc tgtccagcac
     5641 gaagacaggg ttcacatcca gttcagcaag ggacctttca gcaagggcca ttaacgaaat
     5701 atcggagacc atcgccgcaa gggtatcaat atccttgggg gttctccccc tggcaccaga
     5761 aaggaggggg aaacctctca gggaagagat cattcctcga gcctccgccc tgtccacagg
     5821 ggcgtgcctc agagagagat ccttcaggac ctcaacatag atcccgccaa gacccactgc
     5881 gaccaaagga ccgaagacgg gatccctttt cacgccgatc atacactcca ctcctccggt
     5941 aaccatctcc tgcaccagca cccccctgat cctggccccc ggtctggcct tgatcgatct
     6001 ttcaaggagc ctgccatagg cgttcctcag ctcctcttca tccttcagtc tcagcgcgac
     6061 cgctccagcc tcggtcttgt gtatgatgtc gggcgaaacg accttaaggg ctaccgggta
     6121 gccgatctcc ccagcagccg agagagcttc ctccagggaa gcgcagagga tctcccttgt
     6181 caccgggatc ccccaccggg ccaaaagcct ttttgcgtcg tactcggtga ggtccgccgg
     6241 catcttcggc atgaaaggct ctcccaggtc cgagggagcc cttgcgggga gggccggtct
     6301 ggtcgtccat tcagcaagcc tggcaagggc ccaggcacac cttttgggac ttccgtagag
     6361 cggtatcttc tcgtccctta tctgctccag gaacttcccg ccctgttcgc tgttgataag
     6421 ccagcagcag gccaaaggct tcactgcctt cctcgagacc tcgataaggt cacgggccac
     6481 tgccgatccg gagtcccctg ttatcatgga aaggacaact gctgccatgt ctacatccgg
     6541 gcatcccagg accgtctcca tggatgagcg aaaattgcca gggtcattga ttacctgtgc
     6601 tgtgaggtcc accgggttcc gtgtcgatcc gaaggggggt acgaactgct ccagcaatcc
     6661 acgagtccgg ggaggaaggg gatcgacctg caacttgagg tcgaatgccc tgtcgctcat
     6721 tatgatcccc gctcctcccg atgtagtgac gacagccaac ctcttcccct ttggtctttt
     6781 cccgctgccg aagatcttcc ccaggtccac tatgtcatcg gaatcctcga tctcgataac
     6841 tccgtactgc ctgaagatag tcctccatgc ttcgtcctcg ccggtaagga cagcggtgtg
     6901 gcttttcact gccgcctgcc ccgtggggct cttgcccacc ttcaggacgg cgacgggaac
     6961 attccgctct cccgcgaacc tcaacatctc tatgaacctt gccccatccg aaagcccttc
     7021 catgtagagt accagcagtc ggacctccgg atcggcggca aagaccatcc caaaatcaac
     7081 gagatccaga tccgcctggt tcccggtggt gactacatat ctgaagccga caccgctctc
     7141 tgcggccatg gcaaaggagg cgaatccgaa ggcgccgctc tgggaaaggt acgccacgct
     7201 tcccaggggg cgcctgtcac tgtcaaggga agctgaaaag ctcaacggga cggagtcggc
     7261 aaagttgacc agcccctgac agttgggacc cagtattctc atgccccctg acctggcttt
     7321 ttccaggata tcatcctggg cagcgggatc attgctttca gcgaaaccgg aggagaaaat
     7381 tattgaatag gggacgcccc gttcggaaca ttggccgatg acctcaggca caagaacggc
     7441 ttttacaccc accaggacca catctggggt ctcggggaga gatcgtacat cggggaagca
     7501 ttcaagtccc gctacgacct tacggttggg gttgacgggg aagaccctcc ccttaaagcc
     7561 gaagcgcttc agcagtttca ccgggcggcc ggatatgctc tccaggttcc ccgaagctcc
     7621 taccactgcc accgaacgcg gcgagagaag tttcgacagg tcgccggatc ttgctgcggg
     7681 acaagccatc tcaggcaccc tccccggata cggtcattac caggggtgat ccaagaccat
     7741 caatggatac ctccaccctg tcaccggaga atatctcccc tacgccctca ggtgtacccg
     7801 tcaggatcac gtcccctgct tccagggtca tgaattccga aatatatttc aggatcaccg
     7861 aaatgggaaa tatcatgtcc tctgtccgcc cctcctgtct caggacaccg ttgacgaaag
     7921 tcctgaccac ggtcctgtca gggtttatgc ccgtctctat ccatggaccc agtgggcaga
     7981 atgcatcggc cgattttcct cggacccact ggaggtcacg tctctggcag tcccgggcag
     8041 tgagatcgat cccggctgtg tagcccagaa catgatcgat ggggtgttcc gtcccgggaa
     8101 ggatcctttt ccctatcacc acggcgagct ctccctcgta gtggaaatcg ttcgtgaagt
     8161 ggggataggg tatctctgaa aagggagcag ccagagagtt agggcctttc aggaagagac
     8221 ccggctcttc aggtaaggga aggtcgtcgt tgcccatctc cctgatatgg ccgtggtagt
     8281 tgcgccccac gcagacgatc ttggtgggta aggctggcgc gaggagcctc acagaagcca
     8341 ggggtgcgga tccgccggtc ttttctccac caggtgagga tgtaacgaag accctttcgt
     8401 cttcgagcga tccccagtga acatcattgc cctcgttgat tgcaaacctg acgatcctca
     8461 tattgaaaga tcccgcccct ctcacattac aagcccggaa gatcacctta ccaggctctt
     8521 ctgtagatct tatcgatatc ctctctattg atcgttttgg ggttgttcga gagaagtctg
     8581 gcctcccttg aggccgcatc ggccatcgtc gggatggcgt cctccggcac ccccaggtcc
     8641 ctgatcctta tggtcacacc tatcctcctg gccgttcctg tcatccactc tgtcaggtat
     8701 tccgatgctt cctcatctga catgttcaga cgctccatcg agacagcccg ggccagacgg
     8761 gacgttttct ttgcgcatcg gtccctgtcg aactccatta cgctcttcag cataaccgta
     8821 tttgcctctc catgggggat attgaactct ccccccaggg gataggccag ggcatggaca
     8881 gccgcagtac cggccgtggc gaaggccatt tcggccataa ggcttcccag cgacatcccc
     8941 ccgccttgat gcggcatctc ttccatccat cacatcgtcc ttcagagagg aggctattct
     9001 tcgtacggtt tccagtgaaa ggctttcgct aaaggggttg gcgtttaccg acaggaagga
     9061 ctccgcggca tggatcagtg catccatccc ggtatcggcc gtcaccttcg gggggacgct
     9121 ccaggtcagc tccgggtcaa caatggctgc ataagggacc aggaaggagc tgaccacccc
     9181 cttccttacc ttctcctcgg caaaggtaaa ggtcgccaca ggagagacct ctggaccgct
     9241 cccggaagtt gtcggcagca taaccgcggg tagtcccctg cagggcaccc tgtcccatcc
     9301 gaccatggaa aggatatctg cccctgttgc ggccattacg gaagccacct tggtgaggtc
     9361 catggtactc cctccaccta gacctgtcat tatatcgtaa cctgacctgt cattatatcg
     9421 taacctgctc cccttgccgc ttcggccgcc gcttttacga tctcgaccag tgtttccggc
     9481 tcaacgccat tgaatacccc gtaacggata cccgacagat ccagtatctc cctgaccctt
     9541 tccattaagg ggccttctga aatggtcctt ccggacagta tcagggagtg ctcagccttc
     9601 atttcactca aaagggccgg caggtccgaa acagttccgt ccccgaaaga gacccttccc
     9661 gggaagcgga acgagaagcg gtcgcctgta caggcctctg tcactcccat cggatcaccc
     9721 cttgtctgtt atcccgggct tttcccctgc tcctgattag atgattttgc cataaagagg
     9781 gcccctttcc tacaggaagg ggccctctgc gttgaaaact cccgaaagac cttcaaagac
     9841 ggaggtcgtt aatggcgagg gcaagacgtt tcgccgcctc aggaaaatca tcggcattgg
     9901 cataggagaa acaggccctt agatgttcct ccccgcctcc agtggggaag aaggcgtccc
     9961 ccgtcacgaa ggagacgttc ctttctatgg ccttgaaaaa gagatctctg gtgttgaccc
    10021 cgggaagggt cagccaatag aagaaccctc cctgtggggt tccgtaggtc gtgtcaggag
    10081 ggagatactc ccggaaggct gcgtccatgg cgtcccgttt cttcttatag tgctcctgta
    10141 tccgggggag gaataaatcc atgtgtccca gccggcagta ctcgtataca agcccctgag
    10201 ccagaacact ggtgcagagg tctgagccct gcttgaccat tatcatcttg ccgatcactt
    10261 cctcggggcc gacaacccac gcgaccctgg ttccgggggc aagtatcttg gagaatgagc
    10321 aggcgtagat cacaagaccg cttctgtcca tcgagaagat ggttggctca tgttctccat
    10381 cgaaacggag atggccgtag gggtcatcct ccaaaacagg aaccccgaag gtttctgcaa
    10441 tctcgagaag tttttttctc cttgatgccg acagggtgta ccccgaggga ttctggaagt
    10501 ttactatcga atagatgaac ttgaccttgt ctctcccctt tctggcctgt tccaggatct
    10561 ccgggagcat gtcgaccttc attccctcgc tgtcgcatgg aacggttacg aaacgggcct
    10621 ttcggttctt aagggcgtgg gtggctcctg gataggtagg ctcctcgacg atcaccacat
    10681 cgccagggtc aagcatcacc gccgcgaaca ggtccatgac ctgctgggaa ccggtggtga
    10741 tgcacatctc ctctggagcg gtcgccctgc ccatccttgg tgccatccag ccggagatga
    10801 attcctttaa aggttcatat ccccccgtcc cgccgtactg aagaatctcc ttgccccgtt
    10861 cggaaataac ccccgccgct tcctgaaact ccttgacagg aaatatctcc gggtcggggt
    10921 ttcccccagc cagggatatc atgcccggtc tcttcatgta cttgaccagt tccctgatcg
    10981 gggacggcgt caggttgatc cctacttcag aaaaaagccc ctcccaaacc tcctggctca
    11041 ctgggaccac tctccttgat cgtttttttc ttcccgcctt ccgcggtacg gatcgggcct
    11101 gctttccaga tatcctcagg gttctattat cacgactgtt ccgggtacgg atctttcgac
    11161 gatctccatg accgcctcgt tgctcatcct tatgcaaccc tcggtaacca ttttacccag
    11221 tgaagaaggg tcatgagttc cgtgtatccc tatccccttc caccccgtct caagccttat
    11281 gaagaaaggc ccgtaagccc cttcgacagg tcccttcccg tctccgaaat catggaccca
    11341 ggtcctggaa tcgtgggtcc tttcgacgcg gaaaactcct tcaggggttc tcctgtcccc
    11401 caccctctcc ttgttgcccg ggttctctcc gacagcgatc ggaaaaactg cctccctttc
    11461 tgtaccgttc cacgtggaga gttcgaaaca gcccttccct atgaggatcc agaaactttc
    11521 ctcaagaccg ttctctgcta cgaaaacctc tatcctgtca gcatcagggg caggatgctg
    11581 gtcctgttcc gccggggcga tacgttgatc ctctgccgcc tgaatggccc gggttcttgc
    11641 tggcgacaac ctctccagca acagatagcc tcccagcccc agagctgaaa ggatcataac
    11701 aacgacaaag gcgcccggaa gagaccactt cctccgcctc gttgagaaca tggactgccc
    11761 ctcctccttt aacgacatga ggacccaccg ccccctgaca ggtcaccggc aaggcctttc
    11821 agcgctgatt ctggatctga tcctgaacca gctggaccag gtcggcgata tagttaccga
    11881 tcacagggat gttcaacgcg agaatggact tgagaacgat caccagaaag tatatgacca
    11941 ccgcagcaag gactgtcaac cccaccccga aaccaacacc ctttgccagt ccggcaagga
    12001 agttgttcca gatcagttta cgtctgtcca gcagtatctc gaggttggcc attacccggg
    12061 atctctcgat cacttccgca aggcgttccg taacctgttt cttgtcgtct tccgaagggt
    12121 tcttcctgat atctcccata tcgacctcta ttccaccagg atccctacgg ccactgaacc
    12181 tatccctgaa tgggccccaa ggacaggtga tatgtccatt acgtaagcgg gatcacttcc
    12241 aacgatctcc cgcattccgg aagcggccgc ctcagccgaa gccggagaat gggcatgaac
    12301 gacgcaatag ttccagaccc ttgctctccg gcactcctgg tctatgatct cacggatctt
    12361 gttgacgttg gcctcccagc ccctggtctg tccgagggat ctggcgaagc cctcttcgtt
    12421 tacggtgatt atcggcttca ggttaagaag ggtcgcaaga agtcccttgg ctgggcttac
    12481 tctccccccc ctgaccatat acttcagggt cttcaggctg acaaagatct tcgctccagg
    12541 gatcccggcc tcgataaggg agacggtctc ctcgtggcct ttcccttcgt tcagggcctg
    12601 ggcagccctc atgaccagca ggcccagagg agctgaagcc gtcctggaat cgatcacggt
    12661 gatcatcccc cccacctttt ctgcggcagc cctgctggcg ttccatgtcc cgctcagctt
    12721 agacgagata tgcacagcaa tgaccgagtt gtagtgctcg aggaggaacc ggtaggtcct
    12781 ttcgaagtcg gagatggagg gttgggaact tacaggcctc tctccggact cttccaggag
    12841 agtgtagaac tgttcggagg atatggtcac cctgtcgata aagacgctct ccccaaaagc
    12901 cagacggaga ggaaccacgt ggatctggtt cctgtcaagg aattcctggg gcagatcaca
    12961 ggtggagtcc gttacaaggg ccacggagtg catccttttc ctgcagacat caacctgtcg
    13021 cctcatgtca tcggctttct gctgggtgag ggcaccgtgt ttcttgatcg tgaagaacag
    13081 ccggtcaggg gtgtcagtat ggatatg
//
LOCUS       DAOPKQ010000013        12007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_5581_length_12007_cov_4.528196, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000013 DAOPKQ010000000
VERSION     DAOPKQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12007
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5581_length_12007_cov_4.528196"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PK146_01005"
     CDS             <1..724
                     /locus_tag="PK146_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HPE90846.1"
                     /translation="TEGLLLQRCREMNRGFLSRIERGRPWVTLKGAVTLDGNMAMASG
                     ESRWISNAFSRVRSHMLRDSNDAVVVGVGTVISDDPELTVRDTTGRDPVAVVLDPSLR
                     TPQWARIVRRGTIIFFSRSARKEKLEQLEQRGCTLFRVPADMEGRPQLELVLQRLAEV
                     GVNSVLVEGGPNILGSFMTLGLFDSVALFVSTRFSGRGQGIGGGLAIGRLADSIGIRT
                     VRVRNLEGDLWLEGVKPCSQVW"
     gene            706..1353
                     /locus_tag="PK146_01010"
     CDS             706..1353
                     /locus_tag="PK146_01010"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPE90847.1"
                     /translation="MFTGLVECTARIISAKRSGDMVELEIGDVPFAGELKRGDSVSVS
                     GACLSVIVSGRSSFTVEIMPETFDNTVLGKAGPGYRVNLERAMPATGRFEGHIVTGHV
                     DLISRVVALKSRGKSRELDFSLPSDAAGLVVRKGSVAVQGVSLTVIERGDSSFRVGLI
                     PETLRSTTLGVLKIGDPVNVEYDILSKYLKDLVEDRTSDDDGRLTVDRLAGMGWS"
     gene            1350..2615
                     /locus_tag="PK146_01015"
     CDS             1350..2615
                     /locus_tag="PK146_01015"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPE90848.1"
                     /translation="MNRTGEGAGTLAEEGATTTVEEAIEKIRQGGMVIVVDDESRENE
                     GDLVMAAEAADTESINFMVSRARGLVCVPMDDRIARRLDLEPMVRENTDLHSTAFTVS
                     VDAREGVTTGISAEERAITARLLASPEASPEMFRRPGHMFPLIARKGGVLKRAGHTEA
                     AVDLARLAGRSPVGVICEIMKDDGTMARFPDLVRFANTHGLRIITIEELIRYRNRKEK
                     LVEKVAVVNLPTEYGDFIAHAYMFVLEEMKDKVHLALVKGEISPDQDTLVRVHSECLT
                     GDALGSLRCDCGPQLRAAMAMIEAEGNGVLLYMRQEGRGIGLLEKLHAYELQEKGLDT
                     VEANEALGYRPDLRDYGIGAQILADLGLKRIRLITNNPRKIIGLEGYGLEVVDRVPLV
                     ISPNTFNEKYLKTKESKLGHLLHLKDIIG"
     gene            2662..3132
                     /gene="ribH"
                     /locus_tag="PK146_01020"
     CDS             2662..3132
                     /gene="ribH"
                     /locus_tag="PK146_01020"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPE90849.1"
                     /translation="MRIIQGKLVGTGLRFLLAVSRFNELISSRLLEGAKDFLLRHDVQ
                     AQDIEVAWVPGAWELPLVARESALTGNFDAIIALGAIIRGDTPHFDYVASEVSKGLAL
                     VEMEQRVPVSFGVLTCDTLEQALLRAGSKSGNKGADAAAAAIEMADLLRQMRKS"
     gene            3151..4437
                     /gene="serS"
                     /locus_tag="PK146_01025"
     CDS             3151..4437
                     /gene="serS"
                     /locus_tag="PK146_01025"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPE90850.1"
                     /translation="MLDIRLFREKPEELKIALETRNGFFPVEDIIGLDSRRREILTRS
                     DELRSERNQGSREIAELRKKGEDATELMEQMRITGEEIRENDEQLAELEGRIQSLLLE
                     IPNLPHDSVPVGADESFNKVVRSWGEPPVFDFEPLPHWDIGESLGIMDFERGVRLAES
                     RFTVLKGAGARLERALINFMLDIHTREHSFVEVQPPFLVNSDTMKGTGQLPKFAEDLY
                     SCSSDDLWMIPTAEVPLTNLHRGEIIPEEELPLYLTAYTPCFRREAGSHGRDVRGMLR
                     QHQFDKVELVKICSPESSYGELESLTTSAEEILKRLELPFRTVLLSTGDMGFAASKTY
                     DIEVWLPSQEKYREISSCSNCEDFQARRMNTRYRPKGGGKVQFVHTLNGSGIAVGRCL
                     IAILENYQNRDGTVSLPKAIVPYMDGVEVIGERPIP"
     gene            4540..6276
                     /locus_tag="PK146_01030"
     CDS             4540..6276
                     /locus_tag="PK146_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPE90851.1"
                     /translation="MAMDVHEIQGVVFLSLSVFVFFFAIQRISKTTLERDQYQFLKDI
                     CLMAAWGLCGIWSPDPSVRLVVAMGLVSAIIGVGQRVRPKFPWPLGFFIIGVVFASTG
                     LGISFIGFPGGEFLFLNRYSSIMATALWVGIFPLLLQEMDQIPGLAGFLSAVTWIVML
                     IVTGLSRQHIYGAFYMTLVGVVILAVFWSRHGHSYRRLGEPVAALWGTLIAGTSIMGV
                     SKGVTFSTLMLIPLGLFAIPMVEASFNIVSRAFSSRPQGTLFLYRRLVSRGMDHTPAV
                     RAVGSLCAVLGAAVASFQLRDESMALAALLSSTILLGGFLFVVSSGKRRSVPYSSRSF
                     LWGTPVDSVSDDYALGRIRSWVLQGGPGRYIVTLDALSVLRAKEDSSFRQIVGGADLV
                     LPDGRGLTGALGFLGTPVLQRIPGVDFVERMCRLASIEGWPVFFLGGAPGVAEKAAEK
                     LRSLYPGLVIGGIRHGFIAPVDLNGVLEDIRRSGSRFIFVGMGVPRQENWIGEHHSEL
                     EGTVSIGVGGSFDVISGNLKRAPLYLQKAGLEWLFRLVQEPWRWSRVRRLPLFVFHVI
                     LTKLGFSSLKGE"
     gene            6281..6748
                     /locus_tag="PK146_01035"
     CDS             6281..6748
                     /locus_tag="PK146_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPE90852.1"
                     /translation="MTINAGQLMHRDLSSVSEDDHVLDAVHLLFSQRLSGVPVVRDDW
                     VLAGYLSEKDILRKATPTYLEVLAQSSFLDDTEGNLIERLKQIGNMKVKDVMNPDPIF
                     VRPSESLMTVVDLMLRKGLKRLPVVEGRKLVGVIDRGGFCEFMMEGSEFHETR"
     gene            6735..7100
                     /locus_tag="PK146_01040"
     CDS             6735..7100
                     /locus_tag="PK146_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90853.1"
                     /translation="MRPDEPVPTLDDLDPEIESFRRALEVSLEEEKTRLREEQGKALE
                     RIRELSENSITLAESEWEKYEEMLRSSRESFIEELRGRMSYVLDPVLERLMNGDLVER
                     AASGILPAAYRERKEGCRS"
     gene            7097..9004
                     /locus_tag="PK146_01045"
     CDS             7097..9004
                     /locus_tag="PK146_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPE90854.1"
                     /translation="MIRDMVLLGLWGLVRDRSRIIEKLHDLGVLHLQEEPVEYVSTEA
                     AADLKLLRGKALGLLESLGWNEWSSIPRKALEEKRSVLSSPDEAIIDGIRDSLDSFST
                     RVTERIRLREALGSRINSIKGALRTVRHFDSFISENSGKGLEITLWWAQRADVADLAA
                     RIRKMIREHDPLGSDDKTPYHLIVSPGGERILAIAYPSEFRKGVSAILSSRECLPWIL
                     PGEYETMSFQDAVPLMETALEDLPRRIGEIERNLMKTRQEWGPKIGALYVLLDEKIEE
                     ILVETGSDTRGEIFRLSGWVPSDEKDRLETELRGEFRERILLRWRDPHPGEWSEVPTS
                     LSNPEYFRPFELFLRLMPVVSYKGLDPTILIGIFFPFFGGCMIGDMGYGLVIGALGLW
                     FRKKVGRPMLNDVGSILLFIAAWSILWGVAYGEAFGDLGHRFLHLEPLWLERSQVVIP
                     VMIFTISLGFAHVLLGFIMGMIQGLRTGHRHLWLERLGNILIFMALLSGMVLVKGWLP
                     GQFFTISVTLLVLGLALLLAGGGMGGLVEALGSVGNVLSYVRIAAIGLSSAILAMVAN
                     SFVDSMGVTFVGIFMALTIHLLNFVLAIAGSSLHSARLQYVEFLGKFYSGGGTRYKPF
                     SRRKPELWKKH"
     gene            8989..9207
                     /locus_tag="PK146_01050"
     CDS             8989..9207
                     /locus_tag="PK146_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPE90855.1"
                     /translation="MEKALIALAAALAVGLPAIATAYAQARIGSVGAGTIAEKPETGG
                     IIIILEALPETMVILGFVVAVMLILQFA"
     gene            9257..9883
                     /locus_tag="PK146_01055"
     CDS             9257..9883
                     /locus_tag="PK146_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPE90856.1"
                     /translation="MTGTGDFKRALEEEFALKSAEAKAVSDRFVSDRMFEARRETERK
                     VHSIRTRHRKRYELLLEREKRRGLLLVREEALEKISSFLEEISKRVCERISMIRRDRV
                     LYGRILAELVGEALDSLAEEAVVLVPPGEKDLISRDRRIIAVEESEEDTSWGGCLVTD
                     AGTRSVFVDNTIKTRWDLFQKELVRDLSESYCHVLEGSEGSSRELRLP"
     gene            9834..10886
                     /locus_tag="PK146_01060"
     CDS             9834..10886
                     /locus_tag="PK146_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPE90857.1"
                     /translation="MFLKDLKDLPENYGYLNARLRAHFSDFLVKEDYRRLAFRRLEEI
                     EVFLLETRYGKSFRKSLVEQPLSTHRRIEVALADTSADTLNHARKMAMGEPEDLMTVL
                     LARADLHNGRLLLRYYFTGALYEGHPQWHGYGTLPVTFFADLWKSHSITHVIDKCLAF
                     DHQLSRALGRACGQVAKGSSLPASERIFLSGVLTYLDEILDSHRTKDSALVRELLGRS
                     IDIWNMNIWSRVAAGVLPGEKAGELFIEGGYSLSADRLRNSSRPEDLLASTPWETILG
                     RFQGGPAEMVTGRAMREFWKWQIAQRRKDLLGIEVATAFIAHQLLEWQNLNALVVGVD
                     MGLEVDTIIDKLVLTG"
     gene            10892..11167
                     /locus_tag="PK146_01065"
     CDS             10892..11167
                     /locus_tag="PK146_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPE90858.1"
                     /translation="MEKQNYGKGYVLGRQLFIELWSLLGFEAIVCEGPEDFPDCFQKL
                     QSEEVAFVLVESDWAESIPEFYKRKAKTSDPVWVQMPSLKSSVKGWE"
     gene            11173..>12007
                     /locus_tag="PK146_01070"
     CDS             11173..>12007
                     /locus_tag="PK146_01070"
                     /inference="COORDINATES: protein motif:HMM:NF028196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPE90859.1"
                     /translation="MSNMGRIRSLAGTVIRADRDQPVSNFEVARVGNERLLGEVIRID
                     GTEVDIQVYEEIDGVHVGEPVEFTGELLGIDLGPGLLGSVLDGIGRPLLGSFSEDGVS
                     CFLKRGFFSRSLDTKKKWEFTPIRACGDAVLPGDVIGSVIEGEGFRHLILVPPGSGPS
                     RVKEIAPGDRVFSVDDTVCLLENGQEISLCQRWHVRTPRPCEDKLSFSEPLITGQRVL
                     DTLFPVALGGAAVVPGGFGTGKTVTQQSLAKWCNAEVVVYIGCGERGNEMTEVLEEFP
                     HI"
BASE COUNT         2656 a         3009 c         3711 g         2631 t
ORIGIN      
        1 cacggaagga ctcctgctgc agcggtgcag agagatgaac agggggttcc tttcacggat
       61 agagaggggg cgcccctggg tgacactgaa gggtgctgtt accctggacg ggaacatggc
      121 catggcatcc ggcgagagcc gctggataag caatgccttt tcccgtgtca ggtcccatat
      181 gttgagagac agcaacgatg ccgtcgttgt gggggttggc actgtcataa gcgacgaccc
      241 ggaactgacg gtcagggaca cgacggggag ggatcctgtt gcggtcgtac ttgacccgtc
      301 gctccgcaca ccgcagtggg ccaggattgt aaggagaggc accatcattt tcttttccag
      361 gagcgcccgg aaggagaagt tggaacaact cgaacagaga ggatgcaccc tcttcagggt
      421 ccctgctgac atggagggaa gaccccagct ggagctggtc cttcagaggc ttgccgaggt
      481 gggggtcaat agcgtcctgg tggaaggtgg cccaaatatc cttggctctt tcatgaccct
      541 ggggcttttc gacagcgtcg ctctcttcgt ctccaccaga ttttccggaa gaggacaggg
      601 gatcggaggg ggtctcgcga tcggaaggct tgcagattcc atcgggataa gaacagtgag
      661 ggtcagaaac cttgaaggtg acctatggct ggagggggtc aaaccatgtt cacaggtctg
      721 gtagaatgca ctgcaaggat catcagcgca aaaagatcag gggatatggt cgaacttgag
      781 atcggtgatg tcccctttgc cggtgaattg aagaggggtg attcggtctc cgtttcaggt
      841 gcctgccttt cggtgattgt ctctggaaga agcagtttca ccgttgagat catgcccgaa
      901 accttcgata acactgttct cggaaaggcc ggtccaggat accgggtcaa ccttgaaagg
      961 gcgatgcctg ccacgggcag gttcgaagga catatcgtca cgggtcatgt tgacctcatc
     1021 tcgagggttg tagctctaaa aagccgcggg aagagccggg agcttgactt cagccttcca
     1081 tccgatgcgg caggacttgt cgttcgaaag gggtcagtcg ccgtacaggg ggtcagcctg
     1141 acggtcattg aaaggggcga ttcatctttc agggtcggcc ttatcccgga gacactgaga
     1201 tccacgaccc tgggggtact caagatcggt gatccggtca acgtagagta cgatatacta
     1261 tcaaaatacc tgaaggatct ggtggaagac aggacttcgg atgatgatgg cagattgacc
     1321 gtggaccgtc tggctgggat gggctggtca tgaaccggac aggggagggt gctgggacct
     1381 tggcagagga aggggccacc acaacggtgg aggaggcaat agagaagatc cggcagggcg
     1441 gaatggtgat cgtcgtcgac gatgaatcca gggagaacga aggggacctg gtaatggccg
     1501 cagaggcggc cgatacggag agcatcaact tcatggtctc acgggcaagg ggtcttgtct
     1561 gtgtcccaat ggacgacagg atcgccagga ggcttgacct ggaacccatg gtcagggaga
     1621 acacggacct tcattcgacg gccttcacgg tgagcgtcga tgcccgggaa ggtgtcacaa
     1681 cggggatatc ggccgaagag agggcaatta cagcaaggct tctggcctcg ccggaagcgt
     1741 ctccggagat gttcagaagg cctgggcata tgttccccct catcgcaaga aaggggggtg
     1801 tcctcaagag agcaggccat accgaagctg cagtggacct tgctcgactt gcgggcagat
     1861 cgccagtggg ggtcatctgc gagatcatga aggatgacgg aaccatggcc aggttcccgg
     1921 acctggtcag gttcgccaac actcatggac tcaggataat caccattgag gaactgatca
     1981 ggtaccggaa caggaaggag aaactggttg aaaaggtcgc cgtcgtgaac ctcccgacgg
     2041 agtacggcga cttcatagcc cacgcctaca tgttcgtgct ggaggagatg aaggacaagg
     2101 tacatcttgc tctggtaaag ggggagatat cccccgatca ggatactctg gtcagggtcc
     2161 attcggagtg tttgaccggc gatgcactgg gttcgctgag gtgtgactgc gggcctcagt
     2221 tgagggctgc catggcgatg atcgaggcgg aggggaacgg ggtgcttttg tacatgaggc
     2281 aggagggccg cgggatcgga ctgctcgaga aacttcatgc ctacgaactg caggagaagg
     2341 ggctggatac ggtggaggcc aacgaggctc tgggttacag gcccgatctc agggactacg
     2401 gtatcggtgc ccagatactt gccgacctgg ggttgaagag gattaggctc atcacgaaca
     2461 accccagaaa gattatcggc cttgaaggtt acgggctcga ggtcgtggac agggtacccc
     2521 tcgtcatctc gccaaatacc ttcaacgaga agtatctgaa gactaaggag agcaagctgg
     2581 ggcacctgct gcatctgaag gatattatcg ggtagagagt gtgtcccata atattataat
     2641 tcacggggaa gtgatgatgg gatgaggatc atccagggca agcttgttgg tacgggccta
     2701 cgttttcttc ttgcggtctc aaggtttaac gagctcatct cctcgaggct gctcgagggg
     2761 gcaaaggact ttcttctgag gcatgatgtg caagcccagg atatagaagt tgcatgggtc
     2821 ccgggagcct gggaactgcc cctggtcgcc agggagagcg cgctcacggg taattttgat
     2881 gccattattg ctcttggagc catcatcaga ggcgatacgc cccactttga ttacgtcgcc
     2941 tccgaggtat ccaagggact cgcgctggtg gagatggagc agagggtccc tgtttcattc
     3001 ggagtactga cctgcgatac cctggagcag gcgctgctga gggccggcag caagtcaggg
     3061 aacaaggggg cagacgctgc ggctgctgcc atcgagatgg ccgacctttt gagacagatg
     3121 agaaagtcct agaagaggag gaaccccttt atgcttgaca tcaggctctt ccgggaaaaa
     3181 cccgaggaat tgaaaatagc cctggagaca aggaacggct ttttccccgt cgaggatatc
     3241 atcggactgg actcccgtcg gagggagatt cttacacggt cagacgaact cagatccgaa
     3301 aggaaccagg gttcaaggga gatagctgaa ctgaggaaga aaggcgaaga tgcaacggaa
     3361 ctgatggagc aaatgcggat caccggagag gagatacggg aaaacgacga acagctagcg
     3421 gagctcgagg gccgtatcca gtctctcctt ctggaaattc ccaatctgcc ccatgattcc
     3481 gttcctgtag gggcggatga gagtttcaac aaggttgtcc gctcctgggg cgaaccgcct
     3541 gtttttgact tcgagccgct cccccattgg gatatcggag agtccctggg aataatggat
     3601 ttcgagaggg gggtccgcct tgcagagagt cgtttcacgg tactgaaggg tgccggggcc
     3661 agactggaaa gggccctgat caacttcatg ctcgatatcc ataccaggga gcactccttc
     3721 gtggaggtac aacctccctt ccttgtgaac agcgatacca tgaagggtac cggtcagctg
     3781 cccaagtttg ctgaggacct gtactcctgt tcatcggacg atctctggat gatccccacc
     3841 gctgaagtac cgctgaccaa cctccaccgg ggggagataa tccccgaaga ggaactgcct
     3901 ctctacttga cggcctatac cccctgtttc cggagggagg cggggagcca cggcagagac
     3961 gtgcgtggga tgctcaggca gcaccagttc gacaaggttg aactggttaa gatctgctcc
     4021 cccgagagca gttacgggga gctggagtcc cttaccacgt cggcggagga gatcctgaaa
     4081 aggcttgaac tgcccttccg gacggtcctg ctctccacgg gggatatggg ttttgccgcc
     4141 agcaagacct acgacatcga ggtatggcta ccgtcgcagg agaagtacag ggagattagc
     4201 tcctgcagca actgcgagga ttttcaggca cgccgcatga acaccagata tcgtcccaag
     4261 ggtggaggca aggtgcagtt cgttcatacc ctgaacggat cggggatcgc tgtcggaaga
     4321 tgcctcatag ccatactgga gaactaccag aaccgggacg ggactgtctc cctccccaag
     4381 gcgatcgttc cttacatgga cggtgtcgag gtgatcgggg agcggcccat tccctagacc
     4441 caacgcacct atttcatgtt aaaatcataa gggaatgcag aatgaccgga tgaagtgttt
     4501 ttgatccggc tggagatgat cttccgggga ggacgcctta tggcgatgga tgtccacgag
     4561 atccagggtg ttgttttcct gtcgctctcg gttttcgttt tctttttcgc gatacagagg
     4621 atatcgaaga cgaccctgga gagggaccag taccagttcc taaaggacat atgccttatg
     4681 gccgcatggg gtctttgcgg gatctggtcc cccgacccat cagtgcgact ggtggtggcc
     4741 atggggctcg tttcggccat catcggggtt ggacagaggg tcagaccgaa gttcccgtgg
     4801 ccactggggt ttttcattat cggtgttgtt tttgcgtcca cggggttagg aatatccttc
     4861 atcggattcc ccggaggaga gttcctcttt cttaacaggt actcatcgat catggccacg
     4921 gccctttggg tcggtatctt ccctctcctg ctccaggaga tggaccagat cccgggcctc
     4981 gcgggtttcc tgtcggccgt gacatggata gttatgctta tcgttacagg cctttcgagg
     5041 cagcatatct acggcgcatt ttacatgacc ctcgtggggg tcgttatcct cgctgtgttc
     5101 tggagccggc acggtcactc ataccgtcgc ctcggagagc cggtggccgc cctttgggga
     5161 accctgattg ccggcacttc cattatgggt gtcagcaagg gagtcacctt ttccacactg
     5221 atgctgatcc ctctcgggct ctttgccatt ccgatggtag aggcctcttt caacatagtc
     5281 agccgtgcct tctcctcgcg cccccagggc acccttttcc tttacaggag gcttgtaagc
     5341 cgaggcatgg accacactcc ggccgtccgt gccgtggggt cactctgcgc cgtattagga
     5401 gccgcggtgg cctccttcca gctcagggac gagagtatgg ctctggccgc gctcctgtcg
     5461 tcaacgatcc tccttggagg tttcctcttc gtcgtttcgt ctggaaagag gaggtccgtt
     5521 ccatactcct cacgatcctt cctgtgggga acccctgtcg acagcgtctc agacgattat
     5581 gcactgggga ggatccggtc ctgggtcctg cagggtggac ccggccgtta catcgtcacc
     5641 cttgatgccc tgtctgtcct ccgggcaaaa gaggacagct ccttcagaca gatcgtcggc
     5701 ggtgccgacc ttgtccttcc agacggcaga gggctcacgg gagccctggg cttcctcggg
     5761 acacccgtcc tccagaggat ccccggagtc gacttcgttg agcggatgtg cagacttgct
     5821 tccattgagg ggtggcccgt tttctttctc ggcggagccc ccggcgtggc ggaaaaggcc
     5881 gccgagaagc tcaggtccct ttatcccggg ctggtgatcg gcggcatccg gcacggtttc
     5941 attgcacccg tcgaccttaa cggggtccta gaggatatca ggaggtccgg ttccaggttc
     6001 atcttcgtgg ggatgggagt acccaggcag gagaactgga taggagagca tcattcagaa
     6061 ctggaaggaa cagtgtcgat aggagtaggg ggaagttttg acgttatttc aggcaacctg
     6121 aaaagggcgc ccctttatct tcagaaagct ggcctcgaat ggctcttcag gcttgtacag
     6181 gagccgtgga gatggagccg ggtacggcgc ctccctcttt tcgtgttcca cgttatcctc
     6241 acgaagcttg gtttttcttc tctgaaaggg gaatgaagca ttgacgatca atgccgggca
     6301 gctaatgcac agggacctgt catccgtgtc cgaggatgac catgtccttg acgcggtcca
     6361 tctgcttttc agccagcgcc tttcaggggt tcccgtagtg agggatgact gggtacttgc
     6421 gggttacctg tcggaaaagg acatccttcg aaaagcaacg ccgacctacc tggaagtcct
     6481 tgcccagagt tcattccttg atgataccga gggaaacctt atcgaaaggt tgaagcagat
     6541 cgggaacatg aaggtaaagg atgtgatgaa tcccgatccg atctttgtca ggccatcgga
     6601 gagcctgatg accgtagtgg acctgatgct ccgcaaaggg ttgaagaggc ttcccgttgt
     6661 ggaaggcagg aagcttgtgg gggttatcga cagaggcgga ttctgcgagt tcatgatgga
     6721 gggcagtgag ttccatgaga cccgatgaac cagtcccgac cttagatgac cttgatcctg
     6781 agatcgaatc cttcaggagg gccctggagg tcagccttga ggaggagaag acacgcctga
     6841 gggaggagca gggcaaggcc ctggagagga tcagggaact gtcggagaat tcgattaccc
     6901 tggctgagag cgagtgggag aagtacgaag agatgctccg atcctccagg gagtccttta
     6961 ttgaagagct gagaggcaga atgagttatg ttcttgatcc tgtactggag aggttgatga
     7021 acggggatct cgtggaaagg gctgccagcg ggatactccc cgctgcttat cgggagcgaa
     7081 aggaaggctg ccgctcttga taagggatat ggtccttctc ggtctctggg gccttgtaag
     7141 ggacaggtcc cggatcatcg agaagctgca tgatcttggc gttcttcatc ttcaggaaga
     7201 acccgtcgaa tatgtttcaa cggaggctgc ggcagatctg aagcttctta gaggcaaggc
     7261 cctgggtctc ctggagagcc ttggctggaa cgaatggagt tctatccccc gaaaagccct
     7321 cgaagagaag agatccgttc tttcctcccc tgatgaagcg ataatcgatg gaataagaga
     7381 cagtctggac tccttcagca caagggttac cgagaggatc cgcctcaggg aggcactggg
     7441 atcgaggatc aattcgataa agggagccct gagaaccgtc aggcatttcg actccttcat
     7501 ttcagagaac tccgggaaag gccttgaaat cactctctgg tgggcgcaaa gggcagatgt
     7561 cgctgacctt gctgccagga tcagaaagat gataagggaa catgaccccc tgggcagcga
     7621 cgacaagacc ccctatcacc tgattgtcag tccaggcgga gagaggatcc tcgccattgc
     7681 ctatccttcc gagttcagaa aaggggtctc ggctatcctg agctcccgcg aatgccttcc
     7741 atggatcctc ccgggcgagt atgagaccat gtccttccag gatgccgttc ccctgatgga
     7801 gaccgccctt gaagaccttc cccggaggat aggggagatc gaaaggaacc ttatgaagac
     7861 aaggcaggaa tgggggccca agatcggagc cctctatgtt ctgttggacg aaaagataga
     7921 ggagatcctt gtcgagactg gaagcgacac ccggggggag atattccgtc tttcgggatg
     7981 ggtcccttcc gacgaaaagg acaggcttga gactgaattg agaggagagt tccgggaaag
     8041 gatccttctt cgctggcggg atcctcaccc cggagagtgg tccgaagttc ccacgtccct
     8101 cagtaatcca gagtatttca ggcccttcga gctgttcctg aggctgatgc ctgtcgtttc
     8161 atacaagggt cttgacccca ctatacttat cggtattttc ttccccttct tcgggggatg
     8221 catgataggc gacatgggat acggactggt gataggagcc ctcggtctct ggttcaggaa
     8281 gaaggtgggt cgccccatgc ttaacgacgt cgggtccatt cttctcttca tagcagcgtg
     8341 gtcgatctta tggggcgtgg cctacggaga ggcgttcggg gatctgggac accggttcct
     8401 tcacctggag ccgttatggc tcgagagatc ccaggtggtc attcccgtaa tgatattcac
     8461 cataagtctc ggatttgccc acgttcttct gggttttatc atggggatga tccaggggct
     8521 gaggacaggt cacaggcacc tgtggctgga aaggctggga aacatcctga tctttatggc
     8581 cctcctttct gggatggtcc tggtcaaggg atggctgccc ggacagttct ttaccatctc
     8641 cgttaccctg ctggtgctgg gacttgccct tctgcttgca ggaggaggaa tggggggtct
     8701 tgtcgaagct ctggggtctg tcggtaatgt gctcagttac gttcgtatag ccgccatagg
     8761 tctctcttcc gccattctcg ccatggtggc caacagcttc gttgattcca tgggggttac
     8821 ctttgtaggt atctttatgg ccctgacgat ccaccttctg aacttcgtac ttgccattgc
     8881 aggttcgagt ctgcattctg cccggcttca atacgtggag ttcctcggaa agttctattc
     8941 cggaggggga acccgataca aacccttttc aaggaggaaa ccagaattat ggaaaaagca
     9001 ctgatagctc tggctgcagc tctggccgtg ggattgcccg ccatagccac agcatacgcc
     9061 caggccagaa taggcagcgt aggcgcgggg actattgcag agaaacctga aacgggagga
     9121 attatcatca ttcttgaggc ccttccggag acaatggtga tcctgggttt tgtcgtagcc
     9181 gtcatgctga tcctgcagtt cgcatgaacc ggtcctgaaa aggccagaat aaggcgcccg
     9241 agggtcacct gctgaaatga caggaacagg agatttcaag agagctctgg aggaagagtt
     9301 tgcgctgaaa tctgcggagg cgaaggcagt atcagaccgg ttcgtctcgg acaggatgtt
     9361 cgaggcgaga agagagacgg aaagaaaggt ccactccata aggaccagac atagaaagag
     9421 gtatgaactt cttctggaga gagaaaagag gcgaggcctt ctcctggtca gggaggaggc
     9481 ccttgagaag atctcctcct tccttgaaga gatctcgaag agggtatgtg aaaggatatc
     9541 catgatccgg agggacaggg tcctctacgg aaggatcctt gccgaactgg tcggggaggc
     9601 ccttgactct ctggccgagg aggctgtcgt tctggtccct ccaggtgaaa aagacttgat
     9661 ctccagggac cggagaataa ttgccgtgga ggagagcgaa gaagatacat cgtggggtgg
     9721 ttgccttgtc actgatgccg gcactcggtc cgtctttgtt gataacacga taaagaccag
     9781 gtgggacctg ttccagaaag aacttgttcg agacctctcg gagagctact gtcatgttct
     9841 tgaaggatct gaaggatctt cccgagaact acggctacct taacgccagg ctccgggctc
     9901 acttctcgga cttcctggtg aaagaggact atcggcgtct ggcattccga cggcttgagg
     9961 agatagaggt ctttctgctt gagaccaggt acggaaaaag tttcaggaaa agtctggtcg
    10021 aacaaccgct ctcgacccat cggaggatag aggtggcact cgcggacacc tcggcagaca
    10081 ccctcaacca tgccaggaag atggccatgg gggagcccga ggaccttatg acggtgcttc
    10141 ttgccagggc cgaccttcat aacggaaggc tccttctcag gtactacttc accggtgccc
    10201 tctacgaagg tcacccccag tggcacggtt acggaaccct tccggtgact tttttcgcag
    10261 atctctggaa gagccactcc attacccatg tgatcgacaa gtgtcttgcc tttgatcacc
    10321 aactttcgag ggctctgggc cgggcttgcg gccaggtagc caaaggatcc agccttcctg
    10381 caagcgaaag gatctttctg tcgggggtat tgacatacct tgacgagatc ctggacagtc
    10441 acaggaccaa ggattctgct cttgtcaggg agctgctggg gcggtctatc gacatatgga
    10501 acatgaatat atggtcaagg gtcgccgccg gcgtccttcc cggggaaaag gcgggagaac
    10561 ttttcattga aggaggatat tccctctccg cagaccggct caggaattcc tcccggccgg
    10621 aggatctcct cgcctcgaca ccctgggaga ccattttggg acgcttccag ggaggccccg
    10681 cggaaatggt gacgggcagg gcaatgaggg agttctggaa atggcagata gcacagagaa
    10741 gaaaggacct cctcggcata gaagttgcaa cggcgttcat agctcaccag cttctggagt
    10801 ggcagaacct taacgctctc gtggtaggcg tggacatggg tcttgaagtg gatactatca
    10861 tcgacaagct ggtccttacg gggtgacttt tttggagaaa cagaactacg gcaaggggta
    10921 cgtccttgga agacagcttt tcattgagtt atggtccctc ctggggttcg aagctatcgt
    10981 ctgcgaaggt cccgaggact ttccggactg tttccagaag cttcagagcg aagaggtcgc
    11041 ctttgtgctg gtcgaatcgg actgggcaga gagtatcccg gagttctaca agaggaaggc
    11101 gaagacctct gaccctgtct gggtgcagat gccctccctc aaaagcagcg taaagggttg
    11161 ggagtgagcg atatgtccaa catgggaagg atcaggagcc ttgcagggac agtcatacgg
    11221 gccgatcggg accagccggt ctcgaacttc gaggtcgcaa gagtgggtaa tgaacgtctc
    11281 ctcggagagg ttatcagaat agatggaaca gaagtcgaca tacaggtcta cgaagagatc
    11341 gacggtgtcc atgtgggaga gccggtcgaa tttaccgggg aacttctggg tatcgatctt
    11401 ggtcccgggc ttctgggcag cgtcctcgac ggaataggaa gacccctgct cgggagtttt
    11461 tcggaagatg gcgtctcatg ctttctgaaa agaggtttct tctccagaag tcttgatacc
    11521 aaaaaaaaat gggagtttac ccccataagg gcctgcggag acgctgttct tccaggcgat
    11581 gtcataggat ccgtcattga gggagagggt ttcaggcacc tgatcctggt gcctcccgga
    11641 agcggcccgt cgagggtaaa ggagatagcc ccgggagaca gggtgttcag tgtcgatgat
    11701 acagtatgcc tcctcgagaa cggacaggag atctccctct gccagcgctg gcacgtaagg
    11761 acaccacgtc cctgcgagga taagctctct ttctcggaac ccctgatcac tgggcagaga
    11821 gtgcttgaca ctctttttcc cgtcgcgctg ggcggggcgg ctgtggtccc cggtggtttc
    11881 ggcacgggca agaccgtcac ccaacagtca ctggccaagt ggtgcaatgc tgaggtggtc
    11941 gtgtatattg gatgtggaga gaggggcaat gaaatgacgg aggtccttga agagttcccc
    12001 cacatca
//
LOCUS       DAOPKQ010000014        11992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_5591_length_11992_cov_4.736450, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000014 DAOPKQ010000000
VERSION     DAOPKQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5591_length_11992_cov_4.736450"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /gene="dnaB"
                     /locus_tag="PK146_01075"
     CDS             <1..1239
                     /gene="dnaB"
                     /locus_tag="PK146_01075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPE90860.1"
                     /translation="LLPEDFYDGSHRQAFQVMREMAEKGKPVDPLTFLDELSRRGLSA
                     TLGGQAFIAGFVDSVPTTANAEYHARIVRDKSIHRRLIQVGAEVARLGYNEDREIEDV
                     MDQAERAVFEITQRGTQTPFRRVGDVLGPTFRDIEEKFHSPDSSVTGVPSGFDDFERL
                     TGGFQPGSLNIIAARPSMGKTALALDIAMHASVDRGIPVLMFSLEMGAEQLVQRMLGS
                     RARVNVHDLRTGMFHESAWEKLASAAGDLSRAPFFIDDSSVLTTLELRARSRRFRSQV
                     MKATNGLIVVDYLQLMQLTKRTESKQQEVAEISRSLKAIARELEVPVIALSQLSRAVE
                     SRTEKRPQLSDLRDSGAIEQDADLVILLYRSGYYDAPSEESDNTADVIIAKHRNGPTG
                     TVRLTFFKEHARFASQAWIP"
     gene            1266..3104
                     /locus_tag="PK146_01080"
     CDS             1266..3104
                     /locus_tag="PK146_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPE90861.1"
                     /translation="MSKIAGSKVPRKDICHFRPESGCIVPDPVATVLILFVLSVPNLV
                     FSGAFWFETLHLMKWAVVFVPAGVLVAVAGARIAFIKDVRNSFVLDPFGAVWLFLVLF
                     LVLQPLWAPVTSMPTFTREWFFFASLWGFYIVTRNGFDSRRLNLLLWLAALAGAVNVI
                     FAELQIREIHHLFPFIYPTPGKYIGNTGQQNMFGLWVAIAMLGSSWLFMRVGTDEEGG
                     EKKWATVAPSLLLFSVNAWGVWNSTSRSAVISYALGLFVLSLLLVRSMGARKVLVRLA
                     VIIAILTVTFSGTLLFGRGNAFVMKTFQMVEHFQTVGKRDSIWLTTAEMFRMHPVSGV
                     GLGHYKWNYLEAQRRMLEKHPGMKWQYTYWAHSEVFQWFCETGIFGGLILAALGSWWL
                     KCLFSMLKREGPLSPEGVWASSFLFLIWFNALWTRPFHRIEDALWMALAFGLANREVL
                     PSGFSWTEIRKDWILRSLGLTMCAVAVAGLLFFARGMGGDRLLRRAHMAADAAEQRQL
                     IEKAEGRLMVRDIAQRELAYHNIRVAREQKKGELLAEGLMMLHQHFRQQPHSRELARL
                     MAWYSDVREPYLLESVARYLRPGSYSIRDGQLLLSSEDFRFFFGSK"
     gene            complement(3188..5299)
                     /locus_tag="PK146_01085"
     CDS             complement(3188..5299)
                     /locus_tag="PK146_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPE90862.1"
                     /translation="MKEKPSEVFGINVFNDRAMRERLPKQIYQKLQEAVSGGDKLDFN
                     VAEIVATAMKEWAISRGASHYTHWFHPRTDLTAEKHNAFLTVDEFGLPMDSFGGGDLI
                     QSEPDASSLPSGGMRSTFEARGYTAWDPTSPAFVVSGDHGGTLCIPSVFISNDGTPLD
                     MKTPLLKSLSRIETVTLRILKLFGNRNVRWTRVTMGAEQEFFLVDERKASERPDLQYC
                     GRTIMGAPTPKGQQMEDHYFGSIPPRVLAFMQEVDRHMSTLGIVLKTRHNEVAPCQFE
                     FAPQFTEANLAVDQNQILMEQMRQIASRHGFRLLLHEKPFALINGSGKHLNFSMMDSE
                     GRNLLKPSSSQRKNIQFLTLVSGLLLGISRYGGLLRASIANPGNMQRLGGNEAPPAIM
                     SVFLGDVLTGIFDRIEEGLPENLPSKAFINLGLNRLPDVLMDNTDRNRTAPIAFTGNK
                     FEFRAPGASQSPAGPMTMILSLWTWGLEQMAQMIESRTGETDVADAALDAIRNAVLQS
                     SNIRFEGNCYSQEWHVEASRRGLPIAHSTDEALALFLVPENRELLSGLGIFSDREIQC
                     YYETRLEQYVKTLEIEMNVMEDMILTGVLPAITKQIGSETSAFEKIADSFRKGRPPHW
                     EKQIDELVTIKEGLLEGINSMTSLRDSIGRLDLEKRAEILSEKGVPVLEGVRGMCDIA
                     ERTVSREFWPYPRYSEILLSF"
     gene            complement(5430..6089)
                     /locus_tag="PK146_01090"
     CDS             complement(5430..6089)
                     /locus_tag="PK146_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE90863.1"
                     /translation="MGPNGAGRDTTATEQVYRELRRRIFLKELKPGQRLPEAALASTL
                     SVSRTPVREALRILANEGLVDLIPNSGARLASPSLDEVRESYEVREHLEVIAAAKAAR
                     NITPVQLCLLEEAIDEEEGIFVSRDLERYLEVNIRFHRIIAEASRNRTLSEYVANILA
                     RTYVYMVFFESFFDFDTNPSLDEHREIVKALRQRDESLTVDLMRKHVHLSMEGLKKPP
                     E"
     gene            6203..6943
                     /locus_tag="PK146_01095"
     CDS             6203..6943
                     /locus_tag="PK146_01095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPE90864.1"
                     /translation="MYPDLAGKRVVVTGGASGIGFATALRFVQEGSRVAIFDLDRVAL
                     ERVRLELPAVELLVGTDVSDPLSVASAFDEVDSSLGGIDILISNAGISVRNSFADITF
                     EQWEKVLRVNLAGMFLCAREASGRMRSGGVILMTASTNGMEGHPYYADYNASKAGVIL
                     LAKTMALEFAPLVRVNSVSPGYVLTPMQKAEYTPEMLDKVNEKIPIRRHADPSEVAAL
                     FAFLASGQASYITGANIPIDGGETAGLQ"
     gene            6973..7740
                     /locus_tag="PK146_01100"
     CDS             6973..7740
                     /locus_tag="PK146_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPE90865.1"
                     /translation="MIDEKIFRLDGKVAVVTGGSSGIGLGCARFLSEMGSSVVLADID
                     EAGGRSAEAAIGRGSLFVRCDVSSDEDCRNMASAVIARFGQVDILVNCAGMIRRKDVV
                     QLTEKEWDLSIDVTLKGIYLVSRHLIPFMARGGGGSIVNIASGWGIKGGPKAVSYCAA
                     KGGVVNMTRAMAIDHGPQNIRVNSVSPGDTDTPLLRDEARQLGIDETKWLRESAERPL
                     KRLGTPQDIAMAVYFLASDLSPWITGANLVVDGGGTA"
     gene            7767..9158
                     /gene="gcvPA"
                     /locus_tag="PK146_01105"
     CDS             7767..9158
                     /gene="gcvPA"
                     /locus_tag="PK146_01105"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPE90866.1"
                     /translation="MAEKKRVYPYIPNSEPRIREAMLRETGVDSVDELLTDIPAELRM
                     KGRMNLPVPFSAEADLKRHVEGILSRNITSGEYLSFLGAGCYDHHIPAVVDEIIGRSE
                     FLTAYAGEPYEDHGRFQALFEYQSMMAELLDFDVCNVPTYDGAQAASTSLRMASRITG
                     RKKALLAGNMNPDRLRVVQTYLDPVMSVEIIGWERDSGLLDLEDLGSRLATDVAAVYF
                     ENPSYLGAIEDQGEKIARMAREAGALTVVFVDPSSLGVIQPPSRYGADIACGDIQPLG
                     IHMNYGGGVGGFICTRSDRKYVEEYPSRLFGIAPTSGKEWGFGDVAWERTSFANRETA
                     KEFVGTHAALWGIAAAVYLASMGPKGMADLGKVIIQRSLYARKVIGEIPGVSTSTPSA
                     FCFKEFVVDLTRTGRKVGEINEKLLSRGIFGGKDLSEDFPELGQSALFCITEKITMRD
                     IDRLVEVLGDILC"
     gene            9191..10756
                     /gene="gcvPB"
                     /locus_tag="PK146_01110"
     CDS             9191..10756
                     /gene="gcvPB"
                     /locus_tag="PK146_01110"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPE90867.1"
                     /translation="MTSRIRPFHHMRWNEPIIYQLSVEGQRGILVPEAEKEIEQIVGD
                     GLSSIPGNMRRKDLPNLPEMAQPQVVRHFNHLSQENLGADSNIDIGQGTCTMKHSPKI
                     NEVFARSHKITDLHPYQDCSTVQGILEIFYRTEELFREISGLDRCSVQPGGGTHGVVA
                     MVSMVRAWHRDRGEDQQRDEIITTLFSHPADAAVPKAKGYKVVILPPDEEGLPDLEAY
                     KNALSEKTAAIFFTNPEDTGIFNSRILEFTELAHQKGALCCYDQANANGLLGIARARE
                     AGFDMSFFNLHKTFSSPHGCGGPATGLLMVDRKLGVYLPVPVVRRNERTGRYFLDFNL
                     PKSCGKVKAFFGVAPVVLRAYAWIMSLGADGLEEASRIAVLNNNYFMKKMLKVRGCSI
                     SFPRHPGRVEQVRYSWEKLKEETGFGTGDVQRRIADFGTHYWASHEPWVIPEPFTIEP
                     SESYSRDDIDEYLEIMEQISRECYEEPEVIRHAPHNSTVHHIDHEYLDDPGKWAITWR
                     EYSKKYTGYFQER"
     gene            10753..11892
                     /locus_tag="PK146_01115"
     CDS             10753..11892
                     /locus_tag="PK146_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HPE90868.1"
                     /translation="MTEGRSRDRKVLGLIVNPVAGMGGAVGLKGTDGRAVLERALSMG
                     ALPRSPLRVREALEALKRIADDIRILTCSGDMGALESERAGLPTEVLPIGAGPFTSAV
                     DTMEAARKMEASGVDMILFAGGDGTARDIAGVVGTRVPSLGIPSGVKIHSPVFGRNPA
                     MAGELAGLFMSGAKVALEEREVMDIDEEAFRKGTLRASLFGYLLVPSERRFLQGGKAG
                     GGKSVAVELDGIAQEIISRMMPGDLYLVGPGTTTAAVMRRMGCEYSLLGVDAVRDRKT
                     LGTDLPGKYILDLAEPGRTWLILSVIGGQGFILGRGNLQVTPEVIRVVGKERILVAGT
                     PEKLKGLFGKALLVDTGDPLLDLELCGYTRVVTGLGEETLFRVER"
BASE COUNT         2609 a         3236 c         3582 g         2565 t
ORIGIN      
        1 cttctgcccg aggacttcta tgacgggagc caccgtcagg ccttccaggt aatgagagag
       61 atggccgaga agggtaaacc cgtggaccct ctgaccttcc tcgacgaact gtcgagaagg
      121 ggactttcgg ctaccctggg agggcaggca ttcatcgcgg ggttcgtgga ttcagtcccc
      181 acgactgcca atgccgaata tcacgcaaga atagtacggg acaagtccat tcaccgaagg
      241 ctgatccagg tcggggctga ggtggccagg ctgggctaca atgaggacag ggagatcgag
      301 gacgtgatgg accaggccga gagggctgtc ttcgagataa cccagagagg tacccagaca
      361 cccttccgga gggtagggga tgttctcggt cccaccttcc gtgacataga ggagaaattc
      421 cactctcccg acagttccgt caccggggtg cccagcggat tcgacgattt cgagaggctc
      481 acaggaggat tccagcctgg cagccttaac ataattgccg ccagaccctc catggggaag
      541 accgccctgg cgctcgacat tgccatgcac gcctccgtag acagggggat ccctgtgctg
      601 atgttcagcc ttgagatggg cgcagaacag cttgtccaga ggatgctggg atcaagggcc
      661 agggtcaacg tacacgatct ccgaacgggg atgttccatg aaagcgcctg ggagaagctg
      721 gcctcggccg cgggtgatct ttcaagggcg ccctttttta tcgacgacag ctctgtgctc
      781 acaaccctgg aacttcgggc caggtccagg cggttcaggt cccaggtcat gaaagcaacc
      841 aatggcctta ttgtggtcga ttacctccag ctgatgcagc taaccaagag gacagagagc
      901 aaacagcagg aggtcgccga gatctcccgg tccctcaagg cgatcgccag ggagctcgag
      961 gtccccgtga tcgccctctc ccagctctcg agggcagtcg aatccaggac cgaaaaaagg
     1021 ccgcagctgt cggacctgcg tgacagcggc gccatagagc aggacgccga cctggtcatc
     1081 ctgctctatc gttccgggta ctacgatgcc ccctcggagg agagcgacaa caccgccgat
     1141 gtcatcatcg cgaagcacag gaacggcccc accggtactg tcaggctgac cttcttcaag
     1201 gaacatgccc gttttgcaag tcaggcctgg ataccctagg gtcttatggt cacggggtga
     1261 tatgattgag caagatcgcc ggcagcaagg taccccggaa agacatctgt cacttccggc
     1321 ctgaaagcgg ctgcattgtt ccagaccctg tggccacggt ccttatcctg ttcgtgctat
     1381 ctgtccccaa ccttgtattc tcaggtgcct tctggttcga gaccctgcac ctcatgaagt
     1441 gggctgttgt atttgtgcct gcaggtgtgc tggttgccgt ggccggcgcc aggatagctt
     1501 tcataaagga tgttcgtaac agttttgtac tggacccctt cggtgcggtc tggctcttcc
     1561 ttgtgctgtt ccttgtccta cagcccctct gggcccctgt aacgtccatg ccgaccttta
     1621 caagggagtg gttcttcttc gcttccctat ggggtttcta tattgtaacc cgtaacgggt
     1681 tcgacagcag gaggctgaat ctccttctct ggcttgcagc gttggcgggt gccgtaaatg
     1741 ttatctttgc cgagctgcag atccgtgaaa ttcaccatct cttccccttt atctatccga
     1801 cgccaggaaa atacatcggg aacacgggac agcagaacat gttcgggctc tgggtcgcca
     1861 tagcaatgct gggatcctcc tggctattca tgagagttgg taccgacgaa gagggaggag
     1921 agaaaaaatg ggccaccgtg gcccccagcc ttctcctgtt ttccgtaaac gcctggggtg
     1981 tctggaattc caccagcagg tcggcagtta tctcctatgc cctcgggctt ttcgtcctct
     2041 cactcctcct ggtcaggtca atgggtgcaa ggaaggtcct ggtgcgcctg gcggttatta
     2101 ttgccattct cactgtgacc ttttccggta ccctcctgtt cggccggggt aacgccttcg
     2161 tcatgaagac cttccagatg gtggagcact tccagaccgt gggaaaacgt gacagcatct
     2221 ggctcactac cgcggagatg ttcaggatgc accccgtcag cggggttggg ctggggcatt
     2281 acaagtggaa ctacctggag gcccagagga ggatgctgga gaaacatccg ggaatgaagt
     2341 ggcagtacac ctactgggcc cacagcgagg tcttccagtg gttctgcgag acagggatct
     2401 tcggcgggct tatcctcgct gccctgggtt cgtggtggct caaatgtctc ttttcgatgc
     2461 tgaagaggga gggtccccta tcccccgaag gggtctgggc ttcatctttt ctgttcctga
     2521 tatggttcaa cgccctctgg acccgccctt tccacaggat cgaggatgct ctgtggatgg
     2581 cgctggcctt tggtctggcc aacagggagg tccttccctc cgggttctcc tggactgaga
     2641 tcagaaagga ctggatcctg agatccctgg ggttgaccat gtgcgccgtt gctgttgccg
     2701 ggctgctgtt cttcgccagg ggaatgggag gcgacaggct tttgaggagg gcacacatgg
     2761 cggccgacgc tgcagagcag aggcagctca tagagaaggc cgaaggccgc cttatggtga
     2821 gggatatcgc acagagggaa ctggcctatc acaatatcag ggtcgcaagg gagcagaaga
     2881 agggagagtt gctggccgaa gggctcatga tgctccatca gcatttccgt cagcagcccc
     2941 actcgaggga actggcccgt ctgatggcct ggtactcgga tgtcagggaa ccatatctgc
     3001 tggagagcgt agccaggtac ctgaggcctg gcagttacag catcagggat ggacagctgc
     3061 tgctttcctc tgaggatttc cggttcttct tcggctctaa atgagatctt cccacatcga
     3121 acaatcgaat acgggggcgc ctgagcaggt cgcccccgtc gagttaagga ccatttccgg
     3181 ctgctgctca gaaagagaga aggatctcgc tgtaacgggg gtagggccag aactcccggg
     3241 aaactgtcct ctctgcgata tcacacatgc ctctgacacc ctccaggacc ggaaccccct
     3301 tttcggaaag tatttctgct ctcttctcaa gatcgagccg gccaatgctg tccctcaggg
     3361 atgtcatact gttgatcccc tccaggagtc cctccttgat ggtgacaagt tcatcgatct
     3421 gtttctccca gtgggggggg cggcctttcc tgaaagagtc tgcgatcttt tcgaaggccg
     3481 aggtctccga tcctatctgc tttgtgatag ctggcaggac gcctgtaagg atcatgtcct
     3541 ccatgacgtt catctctatc tccagggtct tcacgtactg ctccagcctt gtctcgtagt
     3601 agcactggat ctcccgatca gagaagatgc ccaggccgga gaggagctcc ctgttctcgg
     3661 ggaccaggaa gagggccagg gcctcgtcgg tgctgtgtgc tatcgggagg cctcttctgg
     3721 aggcctccac gtgccactcc tgggagtagc agttgccctc gaacctgatg ttgctgctct
     3781 gcagcacggc attccggata gcatcgaggg cggcatcggc cacgtccgtt tccccggtcc
     3841 gggattctat catctgcgcc atctgctcca gcccccatgt ccagagggac aggatcatgg
     3901 tcatgggacc ggccggtgat tgcgatgcac cgggtgcacg aaactcgaac ttgtttcccg
     3961 tgaaggctat gggggcagta cggttcctgt cggtattgtc cataagaaca tcagggagcc
     4021 ggttgagccc caggttgata aaggccttcg agggaaggtt ctctggaagc ccttcttcga
     4081 tgcggtcgaa aatgcctgta agcacatccc ccaggaaaac gctcatgatg gcggggggag
     4141 cctcgttccc tcctaacctc tgcatgttcc cggggttggc gatggaggcc ctcagaagtc
     4201 ctccataacg ggagatcccc aggagcaggc ccgaaaccag ggtgaggaac tggatgttct
     4261 tcctctggct tgaagatggc ttcaggaggt tgcgaccctc gctgtccatc atcgagaaat
     4321 tgaggtgctt gccgctcccg ttgatcaggg cgaagggttt ctcgtggagg aggaggcgga
     4381 atccgtggcg ggaagctatc tgacgcatct gctccataag tatctggttc tggtccactg
     4441 cgaggtttgc ctcggtgaac tggggtgcga actcgaactg gcagggagcc acctcgttgt
     4501 gccgggtctt gagaacgatc cccagggtgc tcatatggcg gtcaacttcc tgcatgaaag
     4561 caaggaccct agggggtatt gagccgaagt agtggtcttc catctgctgt cccttaggtg
     4621 tgggcgctcc cattatcgtc cttccacagt actgcaggtc aggcctctct gaggcctttc
     4681 tctcgtccac caggaagaac tcctgctcgg cgcccatagt cacacgagtc caccggacgt
     4741 tccggttccc gaagagctta aggatccgaa gggtaaccgt ttctatccgg gaaagggatt
     4801 taaggagagg ggttttcata tcaagggggg ttccgtcgtt gctgatgaaa accgaaggga
     4861 tacaaagcgt acctccatga tcgccgctaa cgacgaaggc tggactcgtc gggtcccagg
     4921 ccgtgtagcc ccgggcttcg aaggtggacc tcatgcctcc tgaaggcagg gatgacgcgt
     4981 cgggctcgct ctggatcagg tcgccgcctc cgaaggagtc catgggaagt ccgaattcgt
     5041 ccactgtcag aaaggcgttg tgtttctcag cggtgagatc cgtcctgggg tggaaccagt
     5101 gagtgtaatg gctggcaccc ctggagatgg cccactcctt catggctgtg gcaacgatct
     5161 cggcaacgtt gaagtcaagc ttgtcaccgc ccgagacagc ctcctgcagc ttctggtaga
     5221 tctgtttcgg gagcctctcc ctcatagccc ggtcattgaa aacgttgata ccgaagacct
     5281 cagaaggctt ctctttcaat gaaaacaccc ctttcagata atgataactg tatatatcag
     5341 aaagagggtg ttcttgcaat agaaaaagcg aatttgatgc aatttaatga tatattgact
     5401 tctaaaaata gataaaagtg gtgtatacgc tattcaggag gcttttttaa tccctccatg
     5461 gaaagatgga cgtgctttct catcagatcg acggtcagac tttcgtccct ttgcctcaaa
     5521 gccttaacga tctcgcggtg ttcatcaagg ctggggttgg tgtcaaaatc aaaaaatgat
     5581 tcaaagaaga ccatgtagac gtaggtcctt gccagaatat ttgctacgta ctcggagagg
     5641 gtgcggttcc tggaggcctc ggcaattatc ctgtggaacc ggatattgac ctcgaggtac
     5701 ctttcaaggt ctcttgaaac gaagatccct tcctcctcgt caatggcctc ttcaaggaga
     5761 cagagctgga cgggggtgat gttcctggcg gcctttgctg cggcgatgac ctccaggtgc
     5821 tccctcactt cgtagctctc cctgacctca tcgagagagg gtgaagcgag tctcgccccc
     5881 gaatttggta tcaaatccac caggccttca ttagccagta tcctgagggc ctccctgacg
     5941 ggggttctgc tcacggaaag ggttgaggca agagccgctt ccggaagtct ctgtccgggt
     6001 ttcagctcct tgagaaagat ccgtctcctc aactcccggt agacctgttc agtcgcagtt
     6061 gtgtcacgtc cggctccgtt aggtcccatt gtcatccctc cgtgggagat cttgtacgct
     6121 gttgactaaa aacctcaaaa gaggtacaaa agaatatagt atacctagta taccacgtat
     6181 actacgaaag gagggccttt gcatgtatcc ggatcttgcg gggaaaaggg ttgtagtcac
     6241 cggaggcgcg agcggcatag gttttgccac cgccttaagg tttgtccagg agggttccag
     6301 ggttgccata tttgacctgg acagggttgc tctcgagcgg gtccgacttg aactgccggc
     6361 tgtcgagctg ctggtgggaa cggatgtctc tgacccctta tcggtcgcct cggccttcga
     6421 cgaggtggac tccagtctcg gtgggatcga tatcctcatt tccaatgccg ggatcagcgt
     6481 caggaacagt ttcgccgata ttacctttga acagtgggag aaggtcctca gggtcaacct
     6541 ggccgggatg ttcctctgcg caagagaggc ttcagggcgc atgagatcag ggggagtgat
     6601 cctgatgaca gcctctacca acgggatgga gggacacccc tactatgccg attacaacgc
     6661 ttccaaggcc ggcgttatcc ttctggccaa gaccatggcg ctggagttcg cccccctcgt
     6721 cagggtcaac tcggtcagtc cgggctacgt tcttacgccc atgcagaaag ccgaatatac
     6781 ccctgagatg cttgacaagg tgaacgaaaa gatccccatc agaagacatg cggatccctc
     6841 agaggtagct gccctcttcg cattcctcgc atccggccag gcatcctata tcactggtgc
     6901 caacataccc attgatggcg gcgagaccgc gggactccaa taggatctcc aatggaaaag
     6961 gaggaagaac agttgatcga tgaaaagatc ttcaggctag acggaaaggt tgccgttgtc
     7021 acaggaggaa gttcgggcat aggactgggg tgtgcccggt tcctttccga gatgggatca
     7081 tccgtggtcc ttgccgatat cgacgaggct ggcgggcgtt ccgccgaggc cgctatcggc
     7141 aggggaagcc tttttgtaag gtgcgacgtc tccagtgacg aagactgcag gaatatggcc
     7201 tccgcggtga tagcccgctt cggccaggtg gacattctgg tcaactgtgc cggaatgatc
     7261 aggagaaagg acgtggtgca gcttacagaa aaagagtggg acctttccat agacgtcact
     7321 ctcaagggta tttatctggt ctcccggcat cttatcccct tcatggcaag ggggggagga
     7381 gggagcatag tcaacatagc ctccggatgg gggatcaagg gggggccgaa ggccgtctcc
     7441 tactgcgctg ccaagggcgg tgtggtaaat atgacaaggg ccatggccat cgatcacggc
     7501 ccccagaata ttcgtgtcaa ttccgtctct cccggtgata ccgatactcc tcttctccgg
     7561 gacgaggcca ggcagctggg aatagacgag acgaagtggc tcagggagag cgctgaaagg
     7621 ccgctgaaaa gactgggtac accccaggac atcgccatgg cggtctattt ccttgcaagc
     7681 gacctctctc catggatcac cggcgccaac ctcgtcgtcg acggcggcgg gacagcctaa
     7741 acggccaggt caggaggtca ttggatatgg ctgaaaagaa aagggtctat ccctatatcc
     7801 ctaattcgga accgcggatt cgggaggcga tgctgagaga aacaggtgtg gatagtgtcg
     7861 acgaactgct gacagacatc cctgccgagc tcaggatgaa gggcagaatg aaccttcccg
     7921 tgccgttctc cgccgaggcg gacctcaaga ggcacgtgga agggatcctg tcaaggaaca
     7981 ttacctcagg ggaatatctc agtttcctcg gagccggatg ctatgaccac cacattcctg
     8041 cagtggtcga tgagataatc ggaaggtcgg agttcctcac agcctatgcg ggggaaccct
     8101 acgaagatca cgggcgtttc caggccctct tcgagtacca gagtatgatg gctgagcttc
     8161 tcgactttga tgtctgcaac gtccctacct atgacggcgc acaggcagcc agcacatccc
     8221 tcaggatggc atccagaata acgggacgca aaaaggcgtt gctggcgggc aacatgaatc
     8281 ccgacaggct ccgggttgtc cagacctacc tcgaccccgt tatgtcagtc gagatcatcg
     8341 gctgggaaag ggactcaggg ctcctggacc ttgaggacct gggatccaga cttgcaaccg
     8401 acgttgcagc tgtctatttc gagaatccct cctacctggg agcgatagag gaccagggcg
     8461 aaaagatagc gcggatggcc cgtgaagcag gggccctgac ggtcgtcttc gttgacccgt
     8521 cctctcttgg tgtgatacag cccccctcaa ggtacggcgc agatatagcc tgtggggaca
     8581 tccagcccct tgggatacat atgaactatg gcggaggggt cggagggttc atatgcacca
     8641 gaagcgacag gaaatacgtt gaagagtacc cctcccgtct cttcgggata gcccctacga
     8701 gcggaaaaga gtggggtttc ggggatgttg cctgggagag gacatccttc gccaacagag
     8761 agaccgccaa ggagttcgtg ggtacccacg cggccctctg gggaatagcc gcggccgtct
     8821 atctagcctc catgggtcct aagggaatgg ctgacctggg gaaggtcatc atacagcgct
     8881 ccctttacgc aaggaaggta attggtgaga ttcccggcgt gtccaccagc actccgtcgg
     8941 cgttctgctt caaagagttc gtggtcgatc ttaccaggac cggcaggaag gttggggaga
     9001 ttaatgaaaa actcctctca agaggcatat tcggggggaa ggacctttcg gaggacttcc
     9061 cggagcttgg gcaaagcgcc ctcttctgca tcaccgagaa gataaccatg agagacatcg
     9121 acaggcttgt cgaggtcctc ggggatatcc tctgctgagc gctgggtccg aagaaaggag
     9181 tgaacatcat atgaccagta gaataagacc tttccatcac atgagatgga acgaacccat
     9241 aatctatcag ttgagcgtcg aggggcaacg ggggatcctg gtgcctgaag ccgaaaagga
     9301 gatagagcag atcgttggtg acggcctctc gtccattccg ggcaacatga gacggaaaga
     9361 tctgccgaac ctgcccgaga tggcccagcc ccaggtggtg aggcacttca accacctgtc
     9421 ccaggagaac cttggcgccg actccaacat agacataggg cagggtacct gtacgatgaa
     9481 gcacagtcca aagataaacg aagtctttgc cagatcccac aagatcaccg accttcaccc
     9541 ctaccaggac tgctccactg tacaggggat cctggagatc ttctaccgca ccgaggagct
     9601 gttcagggag atctccggac ttgaccgctg cagcgtccag cccggcgggg ggacccacgg
     9661 ggtggtagcc atggtatcaa tggtcagggc atggcacaga gacaggggcg aggaccagca
     9721 gcgcgacgag atcataacca ctctcttctc gcaccccgcc gatgcggccg tccccaaggc
     9781 aaaggggtac aaggtggtga tccttccgcc cgacgaggag gggcttcctg atctggaagc
     9841 ttacaaaaac gccctttccg aaaagacggc cgccatattc ttcaccaacc ccgaggacac
     9901 gggtatcttc aactccagga tcctggagtt cactgaactc gcacaccaga agggggcact
     9961 ctgttgctac gatcaggcca acgcaaatgg acttctgggg atcgcaagag cccgggaggc
    10021 aggtttcgac atgtccttct tcaatctgca caagaccttc tcctctcctc acggatgcgg
    10081 aggtccagcc acaggccttc tcatggtgga caggaagctg ggggtttacc tccctgtacc
    10141 ggtggtcagg aggaacgaga ggaccggaag gtacttcctg gatttcaacc tacccaaaag
    10201 ctgcggaaag gtgaaggcct tcttcggggt ggctccggta gttctcaggg cttacgcctg
    10261 gataatgtcc ctcggtgctg atgggctgga agaggcctcg aggatagccg tactcaacaa
    10321 caactacttt atgaaaaaga tgctgaaggt aaggggctgc tccatcagct tccccaggca
    10381 cccgggcagg gtagagcagg tccgctacag ctgggagaaa ctgaaggagg agaccggttt
    10441 cggaaccggt gacgtgcaga ggaggattgc cgatttcggg acccattact gggcaagcca
    10501 tgaaccctgg gttatccccg aaccctttac catagaaccc agcgaatcct attcaaggga
    10561 cgacatcgac gagtaccttg agataatgga gcagatctcc cgggagtgct acgaggaacc
    10621 cgaggtaata aggcatgccc ctcataacag cacggtccat catatcgatc acgaatacct
    10681 tgatgatcca gggaaatggg caattacctg gcgggagtac agtaaaaaat acaccggtta
    10741 tttccaggag agatgacaga aggaagaagc agggaccgca aggtactcgg cctcatcgtc
    10801 aaccccgtcg ctggtatggg aggggctgtg ggcctcaagg ggaccgacgg aagagcggtc
    10861 ctggagaggg cgctgtccat gggagctctc cccaggtcac ctttgagggt gcgtgaggct
    10921 ctcgaggctc tgaagcggat cgcggatgat atccggatac tgacatgcag cggcgatatg
    10981 ggcgctttgg agtcggagag ggccggcctt cctacggaag tcctaccgat aggggcaggt
    11041 ccgttcacct cggccgtgga caccatggag gcagcgagaa aaatggaggc atccggtgtg
    11101 gacatgatcc tctttgccgg gggtgacgga acggccaggg atattgcagg agtggtaggg
    11161 acgagggtgc cctcacttgg tatcccgtcg ggggtcaaga tccattcgcc cgttttcggg
    11221 cgaaaccccg ccatggccgg ggaactggcc gggctgttca tgtccggagc gaaggtggcc
    11281 ctggaggaac gggaagttat ggatattgat gaagaggcgt tcagaaaagg gactctcagg
    11341 gcaagcctgt tcggctacct cctggtccct tccgagagga ggttcctcca gggagggaaa
    11401 gcggggggtg gaaagagcgt tgccgttgaa ctggacggca tagctcagga gatcatctcc
    11461 aggatgatgc ccggagacct gtaccttgtc ggccccggaa caacgaccgc agcggtcatg
    11521 aggcgaatgg ggtgcgaata ctcgcttctg ggagttgatg ccgtcaggga tagaaaaact
    11581 ctggggaccg accttccggg aaagtatatc ctggacctgg cagaaccggg aaggacctgg
    11641 cttatcctgt cagtcatagg aggccagggt ttcatccttg gaagggggaa tcttcaggta
    11701 acccccgagg tgataagggt cgtcggcaag gagaggatcc tcgtcgctgg gactcctgaa
    11761 aagctgaagg gtcttttcgg caaggccctg cttgtcgata ccggtgaccc tttactggac
    11821 cttgaactat gtggctatac aagggtagtg accggcctcg gtgaggagac gcttttccgg
    11881 gtggagaggt agaagagggg gcttgtccgc cagggcaagc cccctcttct acccttaaag
    11941 ggaccttcct gtctttactg ccatctccag ccacttaccc ctgtttacgt cg
//
LOCUS       DAOPKQ010000015        11661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_5787_length_11661_cov_4.522661, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000015 DAOPKQ010000000
VERSION     DAOPKQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11661
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5787_length_11661_cov_4.522661"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1001)
                     /locus_tag="PK146_01120"
                     /pseudo
     CDS             complement(1..1001)
                     /locus_tag="PK146_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582976.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
     gene            complement(1104..1922)
                     /gene="mazG"
                     /locus_tag="PK146_01125"
     CDS             complement(1104..1922)
                     /gene="mazG"
                     /locus_tag="PK146_01125"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPE90869.1"
                     /translation="MDKGPGVHEQMDRLLSIMKRLREPGGCPWDREQTLKSLRSFILE
                     EAYELVDAIEREEPGSICEECGDLLLQVVFVARICMEEGLFDLADSIKALNDKLKRRH
                     PHVFGEERVENSSAVRKNWDLIKQGERRIGKKDSSLFAGIPPSLPALVKSRQIQDRAA
                     KVGFDWEEGDLKPLMDKVQEEIKELHEAVASCDSDNIEEEIGDTFFALVNLSRHLRVE
                     AEFSLQRANRKFEDRFRFIEDSVERSGRPWSDYSLEELEILWDNAKREKAAGTA"
     gene            complement(1915..5055)
                     /locus_tag="PK146_01130"
     CDS             complement(1915..5055)
                     /locus_tag="PK146_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPE90870.1"
                     /translation="MIAAEDEQPIKAGTIGAILQQRSSATVHLPAGTSSWCWTLRDVQ
                     GPHLAVVPDQQRLSEFVSDWKVLFPERELHVLSEIPLTQEHISNEANRIERGSSTEQW
                     RSAAGCIVATPPAVMAPLALPSAGIDIQAGFPLDRDRFLEWAHVSGYRRSDLVWQEGQ
                     FVSRGSIVDIFDPSYRSPLRIEFFDDRIESIHLFDPESQRSRSPVGPTRVLSLAGGIG
                     KRFDEMLPKSASVVLFEPRGLARSAEAYSWIWDRMAADEVLDPLPQWEEVLERFSGHP
                     NCSIARSLEGAVLRIPVMEVQSFRGRLEAAESIIRGWKEDGFEIRLYSSNDLLARWAS
                     DLGIRTEKGTLSKGFVDRFERRVLLSDSDLVGVSPVTREGRIRRIPPREWEERIGELD
                     HVIHEDYGIARSLGIERVTSGGMDRDCLVLLFAENRRLLLPLVQLYKITPYPALSGEL
                     PQLDSLRGTAWKKASNRAREKARETAKQILALYSARETSRKEPLPPDGELVGKFESAF
                     PFEETSDQISSVLAIKKDMEDPLPMDRLLVGDVGFGKTEVAFRAAVKAAEGGRQTAFL
                     VPTTLLAVQHFESFTARITGLPVRAEVLSRFVPRKRQEEIRKDLERGLVDVVFGTHRL
                     LQGDLRFRRLGLIVIDEEHRFGVLHKERLKKLDPSIDVLSISATPIPRTLHMALGGIS
                     NISIISSPPYRRQPVMTYVGPWRDDLFREAVMRERARGGQVFFVHNRIESIESVAARI
                     RMGFPDLRVDIVHGRMEEKKLEEAMLKFSRRETDLLVCTTIIESGLDLPGANTLIVDN
                     AQDLGLAQMYQLRGRVGRREEQAFAFFFYPADRAIPYESLERLEAIAGLSDIGGGFAL
                     AKRDMEIRGGGQLIGTRQHGHIDRIGFNTYFRMLEEEVTSESGRDPDQRVRMDLRLPV
                     VLPSSYVPQSSVRIAIFRRLLRVDSLPEVLDLEKEIRERFGPVPQSVSFMIASAKIRS
                     MGHRNGLQYVRCTLKETQAGGDPERLEVLASGRPGWVLSSGGVLTGPGGYRGLLGLAD
                     MVCARMGILTGEEEDG"
     gene            complement(5068..5679)
                     /gene="pth"
                     /locus_tag="PK146_01135"
     CDS             complement(5068..5679)
                     /gene="pth"
                     /locus_tag="PK146_01135"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPE90871.1"
                     /translation="MKLVVGLGNPGVRYAWTRHNAGWHVLDLLRQRIGAGKHSLSMDS
                     QVWGPRLVDGEQVLLMKPMTYMNLSGNAVRKVFWSLDLGLEDLLVVYDDVALPFGRIR
                     LRGRGSAGGHKGMVSVIANLGSIDFPRLRIGIGSPGVQRELVDFVTDPFSQEELTELP
                     SICEKAGSAVMTWISRGLDEAMREANQPLDISGTSINENGEKG"
     gene            complement(5686..6348)
                     /locus_tag="PK146_01140"
     CDS             complement(5686..6348)
                     /locus_tag="PK146_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPE90872.1"
                     /translation="MDKGTRIRLEMREGTGKQHCKKIRGEGKVPGVLYGPGYPGSMPF
                     CVNTQTIVPIANSGRWETARLDVETPGGKKELCLMRDLQRDPLTGDILHVDFLQLQKG
                     HRVSVNVPVEILGREKCAGIRQGGVFEQLIHEVEMEVLPREIPDSIVIDITALPIEGT
                     VRIADVKLPESAVLTLSPEEVIVTVVHARVEAEVEEGAEEEEETAEVEVVSKGKAKEE
                     EV"
     gene            complement(6394..7362)
                     /locus_tag="PK146_01145"
     CDS             complement(6394..7362)
                     /locus_tag="PK146_01145"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPE90873.1"
                     /translation="MASKLRELKIFSGSAHPEFTRSICENLGVGIATANLFRFSDGEI
                     GVSVEESVRGADVYVVQPTCEPVNENLMELLIMIDVMRRASAYRVNVVTPYFGYARQD
                     RKTKAREPITAKLVANLIEKAGADRVLAADLHAGQIQGFFDIPVDHLTGIPLLAAYFR
                     DTLKRELEQDRLVVISPDIGGVVRARRFAVHLNADLAIVDKRRSHEVANFSEVMEIIG
                     DVSGKTAVLVDDIIDTAGTMVQAAEALLARGAKKVYACATHGVLSGPAIDRISRSPIH
                     QVVLTNTIPLSEEKKIDKITVLSIAPLFAEAISRIHSDHSVSILFR"
     gene            complement(7365..8759)
                     /gene="glmU"
                     /locus_tag="PK146_01150"
     CDS             complement(7365..8759)
                     /gene="glmU"
                     /locus_tag="PK146_01150"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPE90874.1"
                     /translation="MSIPGTGVLVLAAGKGMRMKSETPKVLLRLLDEPLLYYPMRSTD
                     APDFSGRAVVVGHGSETVSQYLQNAWPGTEVVIQQEQLGTGHAVFTARKWISRFERVM
                     IIPGDVPMITGETLARLNSRYLEADEDCTFLVFEPSDPTGYGRVLDTEKGFRIVEEKD
                     AKEHEKKCGRVNSGIYIFRTGSLLEQLENIGRENSQGEFYLTDVIHNLCEEDKRVGMM
                     VCPDPDEMEGVNDPLQLDRVSRIMRRRILDRHMKNGLKCVDSETVWIGPGVSIGDDVT
                     VEPFVQVWGRSDIGSRSRLGSYSIVRNSILGENVHLSGHVVICDSRAGDNSVLGPFTY
                     VRDGSELGEGAFAGKFVEIKKSMIGEGVKVPHLSYIGDATVGKGSNIGAGTITCNYDG
                     ERKNPTTIGEGCFVGSDTMFVAPVSLGDRSCTAAGSVITKDVPAESLAIGRARQKNIE
                     GWARRKRKPDGGGE"
     gene            complement(8874..8948)
                     /locus_tag="PK146_01155"
     tRNA            complement(8874..8948)
                     /locus_tag="PK146_01155"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8913..8915),aa:Gln,seq:ttg)
     gene            9094..9552
                     /locus_tag="PK146_01160"
     CDS             9094..9552
                     /locus_tag="PK146_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPE90875.1"
                     /translation="MWTIEDGIERIRNRGLKITSQRIAILKLLKGRKDHPSAEKIFRE
                     MKKTYPTISFATIYSTAQILQDAGLLQILTIDEHRVFFDPNPHPHAHFRCEKCGALED
                     IPLDEGLLERIRNSSSHDIDTVQVYCYGTCRSCGEQGPTTTRPRKKPRVH"
     gene            9556..9840
                     /locus_tag="PK146_01165"
     CDS             9556..9840
                     /locus_tag="PK146_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90876.1"
                     /translation="MFRIPLALTIVFFALSMVLGVMISRAKSRLPLSAFWGSLLLFLL
                     FSSFSLYSYAPLWIAIAPLIPAAMVTLLFSVSRKNERNRSTGAEEDDEKS"
     gene            9827..10969
                     /locus_tag="PK146_01170"
     CDS             9827..10969
                     /locus_tag="PK146_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90877.1"
                     /translation="MRKASVPGSLTRTMGRPLTLLLFSGALLLVFLTPLFPACAGVLD
                     EEFTITVELTPGGKISVSRADGPDIILGRVKDLPVNSRWPSFTASAWGQEQTVVASAV
                     NAIHILLSVEKDKGRTVSLLPWETVAPAAGPGAAVVTDIKAGRAIFGAWSPPVGSRVI
                     FERKDGEPYRAEGPPRRGDIMKILAVPSQCPFFVELENRPGGRVTAWYSGGPNILGRV
                     IRPLGGTGRFDGTIFQDTGRIRANHPGVIDVCTSPEGLVGGFQIIPLEHAFSREMLGA
                     WKMTQWMIVGPAEIGGPNLKGSGPLFSGGLLPGPSRGETLWDLWSTYGRKPLVLVRLD
                     GGPWSKMPAFTGRQDHALEDVTHVRIYFPFTAEPQGAGQARSPAAR"
     gene            complement(10977..>11661)
                     /locus_tag="PK146_01175"
     CDS             complement(10977..>11661)
                     /locus_tag="PK146_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPE90878.1"
                     /translation="REVHSVMLLKAGPEIGVAATKPFTGQMAAPFLVALQLGRLRGTL
                     PSDEEERLISELIHIPYKVELLLERENEVMELAKRYASKSNFLFIGRGISFPIALEGA
                     LKLKEISYIHAEAYAAGEMKHGPIALLEPDLPVLAVIPRDGLYEKTLSNIQEAKARNA
                     PLIAVCTEGDTIIPQHVESVIEIPWTDEEFTPFLSVVPLQFLAFHIALLKGREIDQPR
                     NLAKSVTVE"
BASE COUNT         2339 a         3581 c         3085 g         2656 t
ORIGIN      
        1 catctccctg cgaacaaggg ggacctccct gagcacgtcc tggaggcggt caagagagtt
       61 ctgctccttc agctggctca ccaggttgga gtacatgccg cctggtatct ggtagatcag
      121 gacgttgata tcgacccctt caagacccat tatcagcttg ctgtacttct ccctgagctc
      181 cttgaaatac cctgataccg ggaccagcct gtcaaggctt atgcccgtct catactcctc
      241 ccccgtcagc gcagccacca tcgtctccgt ggctggctga ctcgtgccca tagcgaaggg
      301 tgaaatggcg cagtccacca cgtcagctcc agcctggacc gaggcgtagt aggccatggc
      361 ggccattccg ctggtgtatg ggagtggacc tggacaggca gggatgtctt cttctttatg
      421 gtcctcacca gttcgtaggc atccacgggg cccagaaggc ctgccatgtc ctttatgcag
      481 atcgaatcgg ctcccatctt cttcatcttc agggacaggt ccgagaaggc ctccagggtg
      541 tgcatcgggg agatcgtgaa ggatatgcac atctggaggt gggagccctc cttcttgacc
      601 tggtcagcgg cgatctccat gttccggagg tcgttcaggg cgtcgaagac cctgacaatg
      661 tctagcccgc ttccgacggc cctcttcacg aactccctta ccacgtcgtc agcgtaatgc
      721 ctgtagccga caatattctg acccctcaaa agcatctgga gcctggtctt gcgaaagtac
      781 ttcctcaatg tgcggagacg ttcccatggg tcttcgtcca ggaagcgcat gcaggtgtcg
      841 aaagttgcgc ccccccagac ctcgagggag tggtagccaa cttcgtccat cctttccgcc
      901 acggggatca tgtcctcggt gcgaagtctc gttgccatga tggactgatg tgcgtccctg
      961 agggctgtct cggtgatgcc tatcttcttt tttctgggca tttccctggg tgtctccttg
     1021 tcaatatccc ccgtttcggg agttgtaatt ttcttctctt ttgttcgcgc gctcatttcg
     1081 tcacctccgg agtatctact cgttcaagca gtgccagcgg ctttctctct cttggcattg
     1141 tcccagagaa tctccagctc ttcaagggaa tagtccgacc atggccttcc gctccgctcg
     1201 acgctgtctt ctatgaagcg gaacctatcc tcgaattttc tgttggctct ctgaaggctg
     1261 aactcggctt caactctcag atgcctggag aggttgacca gggcaaagaa agtatcgccg
     1321 atctcctctt cgatgttatc gctgtcgcag gaggcaactg cttcgtgcag ctctttgatc
     1381 tcctcctgga ccttgtccat aaggggcttc aggtcaccct cctcccagtc gaaaccgacc
     1441 ttggcggccc tgtcctggat ctgcctggac ttgaccagag cgggaagact ggggggtatc
     1501 cccgcaaaga gcgacgagtc cttcttcccg atccttcttt cgccctgctt gatcaggtcc
     1561 cagttcttcc tgacggcaga actgttttcc actctctctt ccccgaaaac atggggatgt
     1621 ctccttttca gcttgtcgtt gagggccttg atggagtctg caagatcgaa cagaccctcc
     1681 tccatgcaga tccgggccac aaatacgacc tggaggagca ggtccccaca ctcttcgcag
     1741 atggaccccg gctcctccct ttcaatggca tcaaccagtt cataggcctc ctccagaatg
     1801 aaggatctca ggcttttcag ggtctgctcc ctgtcccagg gacatccccc tggctctctg
     1861 agccttttca ttatcgaaag aagtctgtcc atctgttcat ggacccctgg acccttatcc
     1921 atcttcttcc tcccctgtca atatacccat gcgggcacag accatatctg caagcccgag
     1981 caagcccctg tagcccccgg ggcccgtcag tacgcctcct gaggacagca cccatcccgg
     2041 ccttcccgag gcgagtactt ccagcctctc aggatcacct ccggcctggg tctccttaag
     2101 ggtacaacga acgtattgta acccattgcg gtgccccatg gacctaatct tcgcagaggc
     2161 tatcatgaag gataccgact gaggtacagg gccaaagcgt tcccggatct ccttctcaag
     2221 gtcaaggacc tctggaaggc tgtccaccct caggagcctg cggaagatgg cgatccttac
     2281 gctggactgg ggaacgtagg aactggggag gaccacggga aggcgtaaat ccatcctgac
     2341 cctctggtcc ggatccctcc ccgactcgga ggtgacttcc tcctcgagca tcctgaagta
     2401 ggtattgaac cctatcctgt caatatggcc atgctgacgg gtgccgatca gctgtcctcc
     2461 gccccttatc tccatatccc tcttcgccag cgcaaaaccg cccccgatat cgctcagccc
     2521 cgcaatggcc tcgaggcgct cgagggactc gtagggtatt gccctgtccg cagggtagaa
     2581 gaaaaaggcg aaggcctgtt cttcccgtct gcccaccctg cctctcagct ggtacatctg
     2641 ggcaagaccc agatcctggg cgttgtctac gataagcgtg ttggccccgg ggagatcgag
     2701 tccgctctca ataatggtgg tacagacgag aaggtccgtc tccctcctgg agaacttcaa
     2761 catcgcctct tccagtttct tctcttccat gcggccgtga acgatatcca ccctcagatc
     2821 aggaaagccc atccttatcc gggccgcaac ggattcgatg gactcgatcc tgttatggac
     2881 gaagaagacc tgcccccccc gcgccctctc cctcatgaca gcctccctga aaagatcgtc
     2941 cctccaggga cccacgtagg tcattacggg ctgacggcgg tagggaggag aggatattat
     3001 cgaaatgttg cttatccctc caagggccat gtgaagggtt ctggggatcg gtgtggcgga
     3061 gatgctcaga acgtcgatcg agggatcaag cttcttcagg cgttccttgt gcaggacacc
     3121 gaaacgatgc tcttcgtcta tgacgatcag acccaacctg cggaacctca gatcaccctg
     3181 gaggagccgg tgggttccga agaccacgtc taccagacct ctttcgaggt ctttccttat
     3241 ctcttcctgt ctctttctcg gcacgaaacg ggagaggacc tcggccctca ccggaagacc
     3301 ggttatcctt gcggtgaatg actcgaagtg ttgaacggcc agaagagttg tgggaaccag
     3361 gaaggccgtc tgtcttccgc cttctgctgc ctttacggcc gcccggaagg ccacctcggt
     3421 cttcccgaag cccacgtcgc cgacaaggag cctgtccatg ggaagggggt cctccatgtc
     3481 cttcttgatg gcaagtacgg acgagatctg gtcactggtc tcttcgaagg ggaaggcgct
     3541 ctcgaacttg cccaccagtt caccgtcggg gggaaggggc tctttcctgc tcgtctctct
     3601 cgcagagtat agggcaagga tctgttttgc ggtctctctt gccttctccc tggccctgtt
     3661 cgatgccttc ttccaggcag tacccctgag actgtccagt tgcggcaact cacctgagag
     3721 ggccgggtag ggagtgatct tgtaaagctg gacgagtggc aggaggagtc ttctgttctc
     3781 tgcgaagagc aggaccagac agtccctgtc catgccgccg gaggtcactc tctcgatacc
     3841 caggctcctg gcaatgccgt aatcttcgtg gataacgtga tcgagctccc cgattctctc
     3901 ctcccattct cttggaggga tacgcctgat cctcccctcc ctggtaacgg gggacacccc
     3961 caccaggtcg ctgtcagaga gcagtactct cctttcaaaa cggtcgacga aacccttcga
     4021 caaggtgccc ttctcggtcc tgatcccaag atccgatgcc cagcgagcca gaagatcgtt
     4081 ggaggaatag agccttattt caaatccgtc ttccttccag cctctgatta tggattcagc
     4141 tgcttcaagt cttcccctga aggactggac ctccattaca gggatccgca gcaccgctcc
     4201 ctccagggac ctggctatcg aacagttcgg atgaccggag aacctctcca gcacttcctc
     4261 ccactgtgga agtgggtcca gcacctcgtc agcggccatc ctgtcccata tccagctgta
     4321 tgcctctgca gacctggcca acccccgggg ttcgaagagg acgacgctgg cgctctttgg
     4381 cagcatctcg tcaaatcttt ttccgatgcc ccccgccagc gaaagaaccc gtgtgggtcc
     4441 gacgggagac ctgcttctct ggctttcagg gtcgaagagg tggatgcttt cgatgcggtc
     4501 gtcgaagaat tctattctga gaggtgaacg ataggaaggg tcaaagatgt cgacgatgct
     4561 tcccctgctt acgaactgtc cctcctgcca gacaaggtca gacctcctgt aacctgaaac
     4621 gtgggcccac tcaaggaacc tgtccctatc gaggggaaag cctgcctgaa tatctattcc
     4681 cgcagaagga agcgcaagag gtgccattac cgcaggaggt gtcgcaacga tacagcctgc
     4741 agcggatctc cactgctcgg tggagctgcc tctttcgatc ctgttagcct cattcgaaat
     4801 atgttcctgg gtcaggggga tctcggaaag aacgtggagt tctctctccg ggaagaggac
     4861 cttccagtcc gagacgaact cggacagtct ctgctggtcg gggactacag caagatgggg
     4921 accctgaacg tccctgaggg tccagcacca cgaggacgtc cccgcgggca ggtggaccgt
     4981 ggctgaggac cgctgttgca gtatcgctcc tattgttcct gccttaatag gttgttcatc
     5041 ttccgcagcg atcatggcga aagaaggcta ccccttctct ccgttctcgt tgatcgaagt
     5101 cccggaaatg tccaggggtt gattggcctc ccgcattgcc tcgtccaacc ccctggagat
     5161 ccacgtcatg actgcgctgc cggccttttc acagatagat gggagctcgg tcagttcttc
     5221 ctgggaaaag ggatccgtaa cgaagtccac cagctctctt tgcactccag gagagcctat
     5281 cccgatgcgc agcctcggga agtcaatgga cccgaggttc gcaatgaccg acaccatccc
     5341 cttgtgcccg ccagcagacc ccctgcccct tagacggatc cgcccgaagg ggagggcaac
     5401 atcatcataa accacaagaa ggtcttccag tcccaggtcg agggaccaga agaccttcct
     5461 gactgcgttg ccactaaggt tcatgtaggt catcggcttc atgagaagca cttgctctcc
     5521 gtcaacaaga cgggggcccc agacctggga gtccatagac agggaatgct tcccggcgcc
     5581 gatccgttgg cggagaaggt cgagaacatg ccagccggcg ttgtgccttg tccaggcata
     5641 acgcaccccc gggttgccca gccccacgac cagtttcaaa aaaagctaga cctcctcttc
     5701 cttcgccttt ccctttgata ctacctctac ctcggcggtc tcttcctcct cttccgctcc
     5761 ctcttcgacc tcggcctcca cgcgggcgtg gactacggtc acgatcactt cttcgggaga
     5821 aagggtgagg accgcgctct ccggaagctt gacgtcggca atgcgcacag tcccttcgat
     5881 gggcaatgcc gttatgtcta tgacaatgga gtcaggtatc tccctgggga gaacctccat
     5941 ctccacttca tggatcagct gttcgaagac gcccccctgc ctgatacccg cgcacttttc
     6001 ccttccgagt atctcgacgg gaacattgac cgaaaccctg tgccctttct ggagctggag
     6061 gaaatccacg tgaaggatat caccggtcag cgggtctctc tggagatctc tcataaggca
     6121 gagctccttt ttccctccgg gggtctcaac gtccagcctg gcggtctccc aacgacctga
     6181 gttggctatg ggaacaatgg tctgggtgtt cacacagaag ggcatagatc cggggtatcc
     6241 gggtccgtag agcaccccgg gcaccttgcc ttcgcctctg atcttcttgc agtgctgctt
     6301 ccctgttcct tctctcattt cgagcctgat cctggtaccc ttgtccataa aattccctcc
     6361 tcgttgtgta taagaaccag aggtcgcagt gatctacctg aaaaggatgc tcaccgaatg
     6421 atcggagtgg atcctgctga tggcctctgc aaaaagcggt gctatcgaaa gaacagttat
     6481 cttgtcgatc ttcttttcct ccgacagggg tatggtattg gtcagtacca cctggtgaat
     6541 gggcgacctg ctgatcctgt ctatggctgg acccgaaagg acgccatggg tcgcacaggc
     6601 gtataccttt ttggcacccc gtgccagaag ggcttcagcg gcctgaacca tcgtgcctgc
     6661 cgtatctatg atgtcgtcaa cgagcacagc tgtcttgccg gaaacgtcac cgattatctc
     6721 cataacctcg ctgaagttgg ccacctcgtg ggagcggcgc ttgtcaacta ttgccaggtc
     6781 ggcgttcagg tgtacagcaa atcgccttgc cctgaccaca cccccgatgt caggcgatat
     6841 taccacaagc ctgtcctgtt ccagttccct cttgagggta tccctgaaat aggccgccag
     6901 gagcggaatg cctgtaagat ggtccacagg gatgtcgaaa aaaccctgga tctgaccggc
     6961 gtgcagatcg gcggccagga cacggtcagc accggccttc tcgatcaggt tggccaccag
     7021 tttcgccgtg atcggctccc gggccttggt cttgcgatcc tgcctggcgt atccaaaata
     7081 gggggtaact acgttaaccc tgtaggcgga tgccctgcgc atgacatcga tcattatcag
     7141 cagttccata agattctcgt taacaggttc acaggtgggt tggacaacgt agacgtcagc
     7201 tcccctgacg ctctcctcca cggaaacacc tatctcgccg tcagagaacc ggaagaggtt
     7261 cgctgtggct atccctacac cgaggttctc gcagatggac ctggtgaact cggggtgggc
     7321 gctccctgaa aaaatcttaa gttcccttag ttttgaggcc accttcattc ccctcccccg
     7381 tcgggtttcc tcttcctgcg agcccaccct tcgatattct tctgccttgc ccttccgata
     7441 gccagggatt cggcggggac atccttcgtg atgaccgaac cggctgcagt acaggacctg
     7501 tcgcccagcg agacgggcgc cacgaacatc gtatcgctgc cgacgaaaca accttcccct
     7561 atggtggtag ggttcttcct ttcgccgtca tagttgcagg ttatagtgcc ggctcctatg
     7621 ttgctgccct ttcccaccgt ggcatcccct atgtaggaga ggtggggaac cttgacccct
     7681 tcgccgatca tgcttttctt gatctcgacg aacttccctg cgaaggcccc ttcgcccagc
     7741 tccgatccgt ccctgacata ggtgaacggg ccaaggaccg aattgtcccc tgccctgctg
     7801 tcacatatca cgacatgacc tgacagatgg acattctcgc ctaaaatgct gttcctgaca
     7861 atagaataac ttccaagcct ggaacggctg ccgatatccg atctgcccca aacctgcaca
     7921 aagggctcta ccgtcacgtc atcaccgatg gacacacccg gtccgatcca gaccgtctcg
     7981 gaatctacgc atttgagccc gttcttcata tgacggtcca aaatacgtct cctcatgatc
     8041 ctcgagaccc tgtcaagctg aagggggtcg ttaacgccct ccatctcgtc cgggtcgggg
     8101 cagaccatca ttcccactct cttgtcctct tcacagaggt tatggatgac atctgtaaga
     8161 tagaactctc cctgggagtt ttcccttccg atattttcaa gctgttccag aagtgacccg
     8221 gtcctgaaga tgtagatccc gctgttcacc ctgccgcact ttttttcgtg ctcttttgca
     8281 tccttctcct ccacgatccg gaatcccttc tctgtatcga ggacccgtcc gtaaccggtg
     8341 ggatcagaag gttcaaagac aaggaaagtg cagtcctcat cagcttcaag gtaccgtgag
     8401 ttcaacctgg caagagtctc ccctgtgatc atgggaacgt ctcccgggat tatcatcacc
     8461 cgttcgaaac gggatatcca ttttcgggca gtgaagaccg catggcccgt tcccagctgc
     8521 tcctgctgga taaccacctc tgtgcctggc caggcgttct gcaggtattg ggaaacggtc
     8581 tcgctaccgt gccccaccac caccgccctc cctgagaagt cgggggcatc cgtcgatctc
     8641 atggggtagt aaaggagagg ttcgtccagg agtctgagaa ggaccttggg ggtttcgctc
     8701 ttcatccgca tacccttgcc cgcggccagt accagtaccc ctgtgccggg tattgacatg
     8761 gcgatccctc cctgaagtaa attcactcta gattttaaca cgcccggagc accttccgtc
     8821 cgggaacaca caacgatcca ggggaagcca agacttcccc tggtcggatc tgctggctgg
     8881 ggtggctgga ttcgaaccag cgatcacgga tccaaagtcc gccgccttgc cagcttggct
     8941 acaccccaac agttaagccc cgcaattata gcctcaccag ggggaatggt gtcaaggcag
     9001 gcggttttcc ggtgtattcc atgttgtgat atagtacact tcatgtaagg acagattcgc
     9061 aaaaaccaac atgggaacgg gaggtcctgg agtatgtgga ccatagagga cggaatcgag
     9121 cgcataagaa accgggggct gaagataaca tcccagagga tagcgatcct gaagctcctg
     9181 aaggggcgga aggaccaccc ctcggcggag aagatcttcc gcgagatgaa gaagacctac
     9241 cccacaatct ctttcgccac catctacagc actgcccaga tcctccagga tgcaggcctt
     9301 ctccagatac tcaccataga cgaacacagg gtattctttg acccgaaccc ccacccccat
     9361 gcccacttcc gctgcgagaa gtgcggagcc ctggaggata ttccgctgga tgaaggtctc
     9421 ctcgagagga taaggaattc ctcatcccat gatatcgaca ccgtccaggt ctactgctac
     9481 ggtacatgcc gcagctgcgg tgaacagggc ccgaccacca caaggccaag gaagaagccc
     9541 cgcgtccact gagtcatgtt ccggatccct ctggcgctga cgatcgtctt ctttgccttg
     9601 tccatggtcc ttggagtcat gatctccagg gccaagagtc gtctgcccct ttcggctttc
     9661 tggggttccc ttctgctttt tttgctattt tcatcttttt ccctgtactc ttatgcccca
     9721 ctctggatcg ctatcgcacc cctgatacct gcagctatgg tcacccttct gttcagtgtt
     9781 tccaggaaga acgaaaggaa caggtctacg ggagcggagg aggacgatga gaaaagctag
     9841 tgtcccagga tcactaacga ggaccatggg aagacctctc acccttctgc ttttctcagg
     9901 ggcattgctt ttggtgttcc tgacccctct gtttcccgcc tgtgccgggg tgcttgacga
     9961 ggaattcacc attaccgtag aactcacacc cggcgggaag atctccgtca gccgcgctga
    10021 cggacctgat atcatcctgg gcagggtgaa ggaccttccc gtcaacagca ggtggcccag
    10081 ctttaccgcc tctgcctggg ggcaggaaca gaccgtcgtt gccagcgctg ttaacgccat
    10141 ccacatcctc ctctccgttg aaaaggacaa gggcaggacc gtaagcctcc tgccctggga
    10201 gaccgtagcc ccggcagccg gcccaggtgc ggcggtcgtg accgatataa aggcaggccg
    10261 ggccattttc ggcgcctggt ccccgccggt gggcagcagg gtgatattcg agcgcaagga
    10321 cggagaaccc taccgtgcag aaggcccccc gcgtcgtgga gatatcatga agatccttgc
    10381 agtaccctcc caatgcccct tcttcgttga gttggagaac cgccctggcg ggcgagtgac
    10441 cgcatggtac agcggcgggc cgaatatcct gggcagggtg atcaggcccc ttggagggac
    10501 cgggagattc gacgggacga tctttcagga tacgggcaga ataagggcga accaccccgg
    10561 ggtcatcgat gtctgtacct ctccggaagg tctggtgggc ggtttccaga tcattcctct
    10621 ggaacacgcc ttctcccgcg agatgctggg agcctggaaa atgacccagt ggatgatcgt
    10681 gggtcctgct gaaataggag ggcccaacct taagggatca ggtcctctct tttccggagg
    10741 ccttctcccc ggcccgtcca ggggcgagac actatgggac ctatggtcaa cttacggccg
    10801 caaaccacta gtcctggtca ggcttgatgg aggtccctgg tccaaaatgc ctgccttcac
    10861 aggcaggcag gatcacgccc tcgaggacgt cactcacgtc aggatctact tccccttcac
    10921 cgcggagccg cagggggcag gtcaagcccg ctcccccgcg gcgagatgaa acccctctac
    10981 tcgacggtaa cgctcttggc gaggttgcgg ggctgatcaa tctctctccc cttcaacagg
    11041 gcaatgtgga aagccagaaa ctgcaagggg accaccgaaa ggaaaggggt gaactcctcg
    11101 tctgtccagg gtatctctat caccgattcc acgtgttggg gaatgatcgt gtccccctct
    11161 gtacagacgg caataagggg agcattcctg gccttggcct cctgaatatt ggacagggtc
    11221 ttctcgtaaa gaccatccct tgggatcacc gccaggacgg gcagatcagg ctccagcagt
    11281 gcaatggggc cgtgcttcat ctcccctgcg gcgtaggcct cggcgtgaat gtaggatatc
    11341 tcttttaact tgagggcccc ctccagggct atggggaagg atatccccct gccgatgaag
    11401 aggaagttgc tcttggatgc atacctttta gccagttcca ttacctcgtt ctccctttca
    11461 aggagcagtt ccaccttgta gggaatatgg atcagctcgc taataagtct ctcctcctcg
    11521 tcggagggta gggttcctct cagcctgccg agctggaggg ccaccaggaa gggcgccgcc
    11581 atctgccccg taaagggctt ggtggcagcc acgcctatct ccggaccggc cttcaggagc
    11641 attacgctgt ggacctccct g
//
LOCUS       DAOPKQ010000016        11452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_5921_length_11452_cov_6.242871, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000016 DAOPKQ010000000
VERSION     DAOPKQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5921_length_11452_cov_6.242871"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1195)
                     /locus_tag="PK146_01180"
     CDS             complement(2..1195)
                     /locus_tag="PK146_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPE90879.1"
                     /translation="MLFSKTFIPTARDAQKNITDPSVARLSRAGYIAADQETRTLRLL
                     PLGFLLWERVISDALDLAREEGIQVAGFEDVPEDAMTVSKKLVKSYRQLPLTFFSGDM
                     KRWIASGLAPEAGSAGDMRDRFLEMAGTLCRHAGLDPRSGETIEDGRKYISLASSKGA
                     WFAKEGFVCSSCSWCGDDASPAGPVERPVGEALPIKEVETPGADTIAELCSQLGVEPS
                     ATMKTMFYAAEQGGTKEVVAVLARGDHQINDRKLSHVLGGSVVRFADPLEIREAVGDL
                     AGYLGPVGLPGGIKVLADRYVEGSQGLVAGANKPGFHLLNVCWGRDFSPSVVADLVSI
                     QEDLPCPACGSRIVPSHLARVAVAGRESGPSELSFQGEGGTACNAYRWEGTLCITPLV
                     LALAG"
     gene            1387..1941
                     /locus_tag="PK146_01185"
     CDS             1387..1941
                     /locus_tag="PK146_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90880.1"
                     /translation="MIRKGMMALAAYSLEPEIQKGSHPEDSFRTGFLNEVLEILSRLQ
                     QEEKIDEFFLLPDFGFDLGVFIGKEEQTRSIFFNLKMYMGAKPRVVEIGDQNGSGPEI
                     ELLQLNTARSALAAGSFRWILVDITKPRGNRRYSIFTTDQAKEGLMGGLNKKKQNSIK
                     LASVMTFPMTWDELSGKLASFLAE"
     gene            1946..2455
                     /locus_tag="PK146_01190"
     CDS             1946..2455
                     /locus_tag="PK146_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90881.1"
                     /translation="MDPLLFLGSFFRRKKLPLTHEDIIKRASSLDDYFKRLQGKRILV
                     FDPPFWGFHDLFIDGKGRVLLVCLKAEGESFAFAGDERGASVMQKFGPGPELNAEEPL
                     EPGILEWILYDDYIVYRGPFFPISRHPYYLGKVAATFPYDGTIDKSTIPGKISELQEW
                     YKAEKEKRP"
     gene            complement(2487..3482)
                     /locus_tag="PK146_01195"
     CDS             complement(2487..3482)
                     /locus_tag="PK146_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPE90882.1"
                     /translation="MSGRPNIAVICPPDEETDHLHLYEKILGDIADIHFLSGAQPQER
                     EEVLAKSDVVVSASCAPSEISPSEIALLKPGGLLQLLYSGADNVPFDLVPENVMIASN
                     AGVFAVPIAEHVLAMVLCLLKRIVPRYEELKKGVFDRKQQNRILRGKTCGILGFGGNG
                     RAIARIMRSAGVKVIAINRSGKTGEPVDEIWDNSRLDDLLRLSDVLVLTTPLTESTRG
                     LIGDRELSLMPSDAILVNVARGAVVDQGALYSHLVSHPGFCAAIDTWWSEPDAHGQFR
                     LEFPFFGLPNFLGSPHVADNVPGTMSLAVTSALENVRGYLEGGKLKGLLDRTDYQ"
     gene            complement(3656..4471)
                     /locus_tag="PK146_01200"
     CDS             complement(3656..4471)
                     /locus_tag="PK146_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HPE90883.1"
                     /translation="MKFCYFDESGMGDEPVLVVAGIDVDASRMRSTRKDWADFLESLS
                     GRTGRPISEFHTRNFYRGNRLSRTIDGTERAEVISAILDWLSSRRHRITFSAVLKSRF
                     EKMRPDNRLKELGTPWSAAAFHCVLTLQKAHQGLKGSRRQTVLVFDREVAEEDRFSVL
                     IKEPPEWSGACYGLSPGSSPLDTLLEAPLFADSRLVLLIQVADMVAYLLRFHAELEEG
                     LSGESYPGEREQISEWAVRIFGMSLPGKFRYPDRCPDGDCSLFWELAPETLRR"
     gene            4626..5159
                     /locus_tag="PK146_01205"
     CDS             4626..5159
                     /locus_tag="PK146_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE90884.1"
                     /translation="MEKKNDKSFERLTIRTGEPRDVPVILRFIKELAEYEGIGNRVTA
                     TERGLMKTLFGDAPAAETAVAMVDGEPVGFAVFYHTFSTILGKRGLHLDDLYISPQWR
                     GKGLGTAMLRWLARLAVERDCGRFEWWCMKDNAPALEFYERIGAMKHDEVFILRMQGE
                     TIASFAEGRKVTLSGEN"
     gene            complement(5137..5613)
                     /locus_tag="PK146_01210"
     CDS             complement(5137..5613)
                     /locus_tag="PK146_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90885.1"
                     /translation="MDWYVRSQTGWGLRWILLGAFGVLFYRARLRAGSWGSIPDSPEF
                     WPLFLAALVLCLAIVVFTSMTVTVRDMEIEVSMGMDLFRKKIPISGISSVEEVNIPWH
                     SVGIKRISGGWLFSVAISGGVDLVMKNGGRFIIGCHDPVGLKAAIVDRMVSSPRKE"
     gene            complement(5633..6067)
                     /locus_tag="PK146_01215"
     CDS             complement(5633..6067)
                     /locus_tag="PK146_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HPE90886.1"
                     /translation="MAGKKSRRVPVDGSAEKVANTVLGDLLREHGLAGAEAGSCENAA
                     SPGTDKNVVKREISPDLAGVGKIVLRVQRKGRGGKTVTVLSTPGGGLVPASLEPLAKD
                     IRKALGCGARVEDGTVVIQGENSERAEKWLRQRGARQVVRGS"
     gene            complement(6094..6276)
                     /locus_tag="PK146_01220"
     CDS             complement(6094..6276)
                     /locus_tag="PK146_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90887.1"
                     /translation="MLAATVRGGAPGSTSNYRLVEGDGVLLWVDRSLLFRGEGIDLDV
                     GFAGERKVVYATNHTP"
     gene            complement(6283..6459)
                     /locus_tag="PK146_01225"
     CDS             complement(6283..6459)
                     /locus_tag="PK146_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90888.1"
                     /translation="MGKKILIVAMLLAVVAFTGYRVVASPSGEGLRMSPEARAEIAEM
                     GDEATLAPEILSSG"
     gene            complement(6507..6878)
                     /locus_tag="PK146_01230"
     CDS             complement(6507..6878)
                     /locus_tag="PK146_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPE90889.1"
                     /translation="MLIIVRLIRFIFPLVVAFLLFRVLSPVFYRWMAQGRPFEESRRS
                     SSPPPPGGRSFRDPYEILGCSPRDSDEKIRKAYRKLVAKYHPDKFIGLELDKEFIDLA
                     ARRFQEIQEAYEQIRRSRGFA"
     gene            complement(6878..7144)
                     /locus_tag="PK146_01235"
     CDS             complement(6878..7144)
                     /locus_tag="PK146_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90890.1"
                     /translation="MSRAFVDEDSEARLNRERLERERKLRDWLAIQEKKLAFLESDPK
                     AEAMDQELREQWLRETCEDIERTRKMLEEFSREGEEQSQAWGHR"
     gene            complement(7202..9079)
                     /gene="htpG"
                     /locus_tag="PK146_01240"
     CDS             complement(7202..9079)
                     /gene="htpG"
                     /locus_tag="PK146_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPE90891.1"
                     /translation="MPDKETYQFQAEARQLLDLMIHSVYSNRDIFLRELVSNASDALD
                     KLRFEALTDENLAPLAEDLHIIIETDPGAGTLTISDNGIGMNRSELVDFIGTIARSGT
                     KEYVRVLRERGEGSFPEELIGQFGIGFYSSFMVAERVSLVTRRADEGTAWKWESPGDG
                     TYTLEEVSREGQGTTITLFLKKINGDETSRDYSDPSVVREIIRRYSDFVAWPIRMKSA
                     DGSLENLNSMKAIWTRPEGEVSEEEYEEFYRHISHDWNKPARRIFFKAEGGMEFRALL
                     FIPSKAPLGIFIRDVFRGIQLYIRRVFIMDDCRELIPEYLRFLKGLVDSEDLPLNISR
                     EMLQQSPQVETIRKSVARKVLDSLSGMLKEDRETYHSFWKEYGKVLKEGIFSDSRNRD
                     RILEISLFDTTGTDGPVTLDEYLARMPEGQDSIYYVTAPSLTVGRNAPHLESFTERGH
                     EVLVLSDPVDEIWSPVVLEYKGKKFSSVSKGAVQPPSKGGPEADVKVAPPEDLESLLK
                     DLGDVLSDDVKDVRVSERLRTSPSCLVGEAFDLTPQMEAFLRAAGQDVPKVKRSLEIN
                     PSHPLVEKLEKIHQEDPSDPRVSRFARVLYGLAALSEGGSLVDPAAFSREVAELLAEP
                     L"
     gene            complement(9221..9649)
                     /locus_tag="PK146_01245"
     CDS             complement(9221..9649)
                     /locus_tag="PK146_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPE90892.1"
                     /translation="MRQMVPFGRGTSIFDEMDDVFRGFSDAIRPMTRRYVPVDMYEEN
                     NEVVVNIDAPGFDPSQIEIKTFSDRVNVRSNVEESSEGENDGRTWYMRRGNRTMNLSL
                     TLPTEVDPESAQASFKNGVITVRLPKSRAVQGRVLELRQE"
     gene            complement(10055..10750)
                     /locus_tag="PK146_01250"
     CDS             complement(10055..10750)
                     /locus_tag="PK146_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPE90893.1"
                     /translation="MKTIGLIGGLTWESTIEYYRIINEEVARRLGGVHSARILMHSFD
                     FQDIDTPMLEGRWDEIGARVAEAATGLERQGADFLLICSNTIHKVANFASEAVSIPLL
                     HLVDVTASAVKVQGFSKVGLLGTIFTMEQDFYKGRLADLHGLEVLVPEKEDRDFVNRV
                     IDEELTFGNIKETSRERFVGIIDGLASRGVQGVVLGCTEIPLLVRQSDTHVPVFDTTR
                     LHSLAAVEESLKE"
     gene            complement(11233..>11452)
                     /locus_tag="PK146_01255"
     CDS             complement(11233..>11452)
                     /locus_tag="PK146_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE90894.1"
                     /translation="IMPGINGKHTLLYGVEVKFYSLKLALDRDLMTNIPGLFASGDGA
                     GVTRGVIQASASGIVAARGILKSVTRDS"
BASE COUNT         2420 a         3364 c         3059 g         2609 t
ORIGIN      
        1 cctatcctgc aagagccagc accaggggag ttatgcacag ggtaccctcc cagcggtagg
       61 cgttacatgc ggtcccgccc tccccctgaa aggacagctc agagggccct gactctctgc
      121 cagcgacggc gacacgggcc aggtgagagg gcacgatcct ggatccgcag gccgggcacg
      181 gtagatcttc ctgaatgctt acaagatcag caactacgga gggtgaaaaa tcacgccccc
      241 agcagacgtt cagaaggtga aacccgggtt tgttggcccc tgccacgagc ccctggcttc
      301 cctccacgta cctgtcggcg aggaccttga tccccccggg cagaccaacg ggaccgaggt
      361 agcccgcaag gtctcccaca gcctccctga tctccagggg gtcggcgaac ctgaccaccg
      421 atccgccgag gacgtgggaa agcttcctgt cgtttatctg gtggtcaccc cttgccagga
      481 ccgctaccac ttcctttgtg ccgccctgtt cggcggcgta gaacatggtc ttcattgtgg
      541 cggaaggctc tacccccagc tggctacaga gttctgcaat ggtgtccgcc ccgggcgtct
      601 cgacctcttt tatcggtaac gcctccccaa cgggtctctc gaccggacct gcaggagagg
      661 catcatcacc gcaccagctg caggaggaac atacgaaacc ttcttttgca aaccaggctc
      721 cttttgagct tgcaagagag atatacttcc tcccgtcctc gatcgtctcc cccgagcggg
      781 ggtcaagacc ggcatggcgg caaagggtgc ctgccatttc gaggaacctg tccctcatat
      841 cccctgccga acctgcctcg ggggctaagc ctgaggctat ccatcgtttc atatccccag
      901 agaaaaaagt aagggggagc tgcctgtagc ttttcacaag cttttttgaa acggtcatgg
      961 catcttccgg tacatcctcg aaacccgcga cctggattcc ttcctccctt gcaaggtcaa
     1021 gggcatctga tatgacgcgc tcccacagca gaaagccgag gggcagcagc ctcagcgttc
     1081 tcgtctcctg gtcagctgca atgtaacccg cccttgaaag acgagctacc gaagggtcag
     1141 tgatgttctt ctgtgcatcc ctggctgtgg gtatgaaggt ctttgaaaaa agcatgacaa
     1201 tgtacctcct gcggtcaaat gattttccag atccacgcat atccatctgt aacataagta
     1261 taggccatgc cgccaggaag gccagttttt tcattatcta acattgttgg gttataatcg
     1321 gatacgtatg gaccgcagtc gggtaaactc agctcccccg aatggatgaa ccgggggagt
     1381 acgcttttga taaggaaggg gatgatggct ttggcggcat acagccttga gcccgaaatt
     1441 cagaagggat cacaccccga agacagcttc cgtaccggat tcctgaacga ggttctggag
     1501 atactatcaa ggctgcagca ggaggagaag atagatgagt tcttccttct gcccgatttc
     1561 gggttcgacc tgggcgtttt cattggcaaa gaggagcaga ccaggtcgat cttctttaat
     1621 ctcaagatgt atatgggggc gaaaccgagg gtggttgaga taggcgacca gaacggatca
     1681 gggcccgaga tcgagctcct gcagttgaat accgccagga gtgcgctggc ggcgggatcc
     1741 ttccgttgga tactggtgga tataaccaaa ccccggggta accgcaggta cagcattttc
     1801 acaaccgacc aggccaagga aggcctgatg ggaggcctga acaagaagaa gcagaacagc
     1861 atcaagctgg cctcggtaat gaccttcccc atgacctggg atgaactgtc gggcaaactg
     1921 gcatcgttcc tggccgaatg acctcatgga cccgctcctt tttctgggca gttttttccg
     1981 gaggaaaaag cttccactga cccacgaaga cattatcaaa agggcatctt cccttgatga
     2041 ctatttcaag cgcctccagg gcaaaaggat cctggtcttc gatcctcctt tctggggctt
     2101 ccacgatctg ttcatcgatg gaaaagggag ggtcctcctg gtctgcctca aggccgaggg
     2161 ggaatcattt gctttcgccg gtgacgaacg cggggcgtcc gtgatgcaga agttcggccc
     2221 cggccccgaa ctgaatgccg aagaacccct tgaacccggt atcctggaat ggatccttta
     2281 tgacgactat atcgtctaca ggggaccgtt ctttcctatc agccggcacc cctactacct
     2341 tgggaaagtc gcggctactt tcccttacga cggtacgatc gacaaaagca ctattcccgg
     2401 gaagatctcc gaacttcagg aatggtacaa ggcagaaaag gagaaaaggc cgtgaacggg
     2461 gaacagatta agccggcctt gcgacactat tggtaatcgg tcctgtcaag cagccccttc
     2521 agttttccgc cttcgagata tcccctgaca ttctcgagtg ccgacgtgac agccagggac
     2581 atcgtccccg ggacattgtc agcaacgtga ggagaaccca gaaagttagg caatccaaag
     2641 aaagggaact caagcctgaa ttgaccgtgg gcatccggct ctgaccacca ggtatcaatg
     2701 gccgcgcaaa aacccggatg ggaaacaagg tgactgtaaa gtgccccctg atccaccact
     2761 gctccccttg ccacattcac caggatcgcg tccgaaggca tgagagacag ttccctatcc
     2821 cctatgagac ccctcgtaga ctctgtcaag ggggtcgtaa gcacaagaac atcggataaa
     2881 cgcaagaggt catctaatct gctgttatcc catatttcgt cgactggctc tccggtcttg
     2941 ccactcctgt ttattgcaat gacctttact cctgcagatc tcattattct cgctatcgcc
     3001 ctgccgttgc ctccgaatcc aaggatcccg caggtcttcc ccctgagaat cctgttctgt
     3061 tgctttcggt caaagactcc tttctttagt tcctcgtatc tcgggacgat ccttttcaac
     3121 agacaaagca ccatcgcaag aacgtgctcg gcaataggta cggcaaaaac ccctgcgttg
     3181 gaggcgatca ttacattctc cgggaccaga tcgaacggaa cgttgtcagc cccggaataa
     3241 agaagctgaa gcaatccccc gggcttcagc aaagctatct ccgatggtga gatctccgaa
     3301 ggcgcgcaac tggctgagac gacaacatcc gatttggcaa gcacttcttc gcgttcctgc
     3361 ggttgagcgc cgctcagaaa atggatgtca gcgatgtccc caagaatttt ttcatacagg
     3421 tgcagatgat ccgtctcctc gtcggggggg cagatcacgg caatattcgg acgtccactc
     3481 aaaacgaatt cctcctcctt aacaggtgtc accccgtaca cttttgacaa atcctgttta
     3541 ccctctctgt agattgctcg ggatttccca aagaagacag gataaaaacc ggaaagtcct
     3601 ggaaaaccct ttttgtaaaa agtgtaggtc tgacaccttc ttttttcacc ttcttttacc
     3661 ttctcaacgt ttccggggcc agttcccaga agagggagca gtccccgtcg gggcacctgt
     3721 ccgggtagcg gaatttcccg ggaagggaca taccgaagat cctgacggcc cattcgctga
     3781 tctgctccct ttcacccggg tagctctccc cggaaagacc ctcctccagt tcggcgtgga
     3841 aacgcaacag gtaggccacc atatccgcca cctggataag gagaacgagc cttgaatctg
     3901 cgaagagagg cgcctccaaa agggtgtcca gcggcgatga acccgggctg agaccgtaac
     3961 aggctcccga ccactcggga ggctccttga taagcacgct gaaacgatcc tcctcggcga
     4021 cctctctgtc gaagaccagt actgtctgcc tgcgggagcc cttgagaccc tggtgcgctt
     4081 tctgaagggt cagcacgcag tggaatgcgg cggcgctcca cggcgtgcca agttctttca
     4141 gcctgttatc gggcctcatc ttttcaaagc ggctcttgag aactgcgctg aaagtgatcc
     4201 tgtggcggcg ggatgagagc cagtccagga tagccgagat cacctctgcc cgctctgtgc
     4261 cgtcgatggt cctggaaagc ctgttgcccc tgtagaaatt cctggtgtgg aattcggaaa
     4321 tgggccgtcc cgtcctcccg gaaagagact ccaggaagtc ggcccagtcc ttccttgtgg
     4381 accgcatgcg gctggcatcc acgtcaattc ccgcaacgac caggaccggc tcgtccccca
     4441 ttccgctctc gtcgaagtag cagaacttca ttctttggac ctccaaaggt caggtttgat
     4501 caacggtttc aacccttttt attgctgatc cccggcaaaa ctttctcctc gcaactattg
     4561 tattatcata agcgaactga tgggcggatc ttatcacccc ccttcagtct gaggaggtat
     4621 ggtcgttgga gaaaaagaat gataaatctt ttgagcgttt gacgataaga acgggagagc
     4681 cccgagatgt gcctgtgatc ctgcgtttta taaaggagct ggcggagtat gaagggatag
     4741 ggaatcgggt gaccgccacg gagaggggat tgatgaagac cctctttggg gatgcccctg
     4801 cggcagagac cgccgtggcg atggtggatg gggaacccgt gggctttgcc gtcttctacc
     4861 acaccttctc gaccatactg ggcaagagag gtcttcacct ggacgacctt tacataagcc
     4921 cccagtggcg ggggaaaggt ctgggtacgg caatgctccg atggctggcc agactggccg
     4981 tagagaggga ctgcggaagg ttcgagtggt ggtgcatgaa agacaacgcc ccggcacttg
     5041 agttctacga aaggatagga gccatgaaac acgacgaggt cttcatcctg aggatgcagg
     5101 gggaaacgat agcatccttc gccgaaggtc gcaaggtcac tctttccggg gagaactgac
     5161 catcctgtcg actatggcgg ccttcagtcc gaccgggtcg tggcagccga tgatgaaccg
     5221 tccgccgttt ttcatgacga ggtccactcc gcctgagatg gccacgctga agagccagcc
     5281 accggatatc ctcttgatcc ccacggagtg ccagggaata ttcacctctt ccacggaaga
     5341 tatccccgag atcgggatct tcttgcggaa caggtccatc cccatggata cttcaatctc
     5401 catatccctt accgtcaccg tcatggaggt gaagacaacg atcgccaggc aaaggaccag
     5461 agccgccagg aagaggggcc agaactcagg ggaatcgggt atggaacccc aggatccggc
     5521 ccttagtctg gccctgtaga agaggacccc gaaggccccg agcagtatcc atcttagacc
     5581 ccagccggtc tgggaacgga cgtaccagtc catagacctg acctccagat gttcaggaac
     5641 ctctgacaac ctgcctggcc cccctttgtc ggagccactt ttcagcgcgc tcggagttct
     5701 ccccctggat gaccacagtg ccgtcctcta ctctggctcc gcatccgagg gccttgcgga
     5761 tatcctttgc cagcggctcc agagaggcgg gaacaagtcc tccgccgggg gtggagagga
     5821 cggtaacggt cttgccgccc cgacccttac gctggaccct gagaacgatc ttccccactc
     5881 cggcaaggtc gggggaaatc tctcgcttta cgacattctt gtccgtgcct ggtgaagcag
     5941 cattttcgca agacccggct tcggcccccg ccaggccatg ttctctcagg agatccccca
     6001 ggactgtatt ggccaccttc tcggcagaac cgtcgacggg gaccctcctg gacttcttcc
     6061 ctgccatccc atttccctcc cgggatcaga ccctcagggg gtgtggtttg ttgcgtatac
     6121 gaccttcctt tcgcctgcga aacccacatc cagatctatg ccttcacccc tgaaaaggag
     6181 ggagcgatcc acccagagga ggaccccatc cccttcgaca agcctgtagt tcgatgttga
     6241 cccaggggcg ccgccacgga ccgtggcggc aagcacgctt gctcagccgc ttgaaagaat
     6301 ctcaggggca agggtcgcct cgtcgcccat ctcggcgatc tcggcccgtg cctcgggaga
     6361 catccgcagg ccctctccag acggggaggc cactaccctg tagcccgtaa aggcaacgac
     6421 tgccagaagc atcgcaacta tcaatatctt ctttcccatt gtctcaactc tcctctcttg
     6481 aaggggaact gaagtgatac gacacctcag gcgaaacccc ttgatcgcct tatctgttca
     6541 taggcctcct ggatctcctg gaacctcctt gcagcaaggt cgatgaactc cttgtccagt
     6601 tcaagaccaa tgaacttgtc ggggtggtac tttgcgacca gtttccggta ggcctttctt
     6661 atcttttcgt cgctgtcccg gggtgaacac ccgaggatct cgtaaggatc cctgaaagat
     6721 cttcctcccg gggggggtgg agacgaactt cgacggctct cttcgaaggg ccttccctgg
     6781 gccatccagc ggtagaatac cggagaaagg accctgaaga gcagaaacgc cactaccagc
     6841 gggaagatga accttatcag gcggacgatg atgagcatca gcggtgcccc caggcctgag
     6901 actgctcctc gccttcccgg gagaactcct ctagcatctt acgggtacgt tcgatatctt
     6961 cacaggtctc ccgcagccac tgttccctca gctcctggtc catagcctcc gccttggggt
     7021 ccgactccag gaatgcgagc ttcttctcct ggatggccag ccagtcgcgg agctttctct
     7081 cacgttcaag tctctcccgg ttgagacggg cctcgctgtc ctcatccacg aacgccctgg
     7141 acacggagtc caccccccct gagatcatga attacatggc cgcaacgtca tggtcaatca
     7201 attatagcgg ctccgcaagc agttcggcga cctctctact gaatgcagcg gggtcgacca
     7261 ggcttcctcc ttcggagagg gcggcaagac cgtaaaggac cctggcgaac cttgaaactc
     7321 tcgggtcaga gggatcttcc tggtgtatct tttccagctt ttccaccagt gggtgagagg
     7381 ggttgatctc aaggctcctc ttcactttcg gaacatcctg acccgccgcc ctcaaaaaag
     7441 cttccatctg cggagtaagg tcaaaggcct ctcccactag acaggacgga gatgtcctga
     7501 gacgctccga taccctcacg tcctttacat catccgagag gacatcgcca aggtccttga
     7561 gcagggactc caggtcttcg ggcggagcaa ctttgacatc tgcttccggt ccccccttcg
     7621 aaggaggttg gaccgctccc ttcgaaacgg aggagaactt ctttcccttg tattccaaga
     7681 ccaccggcga ccagatctcg tccacggggt ccgaaagcac caggacctcg tgacctcttt
     7741 ctgtgaagga ttccaggtgc ggggcgttcc tccccaccgt caggctgggc gccgtgacgt
     7801 agtaaatgga gtcctgaccc tcgggcattc tggcaaggta ctcgtcgagg gtcacgggcc
     7861 cgtcggtgcc tgtggtgtcg aaaagtgata tctccaggat cctgtcccgg ttcctgctgt
     7921 cagagaagat cccttctttt agtaccttgc cgtactcttt ccagaaggaa tggtaggttt
     7981 cccggtcctc cttcagcatt cccgacaggc tatccaggac ctttcttgcc acgctcttcc
     8041 ttatggtctc tacctggggg ctctgctgaa gcatctccct tgatatgttc agggggaggt
     8101 cctcggaatc caccaacccc ttgaggaacc ttaggtattc gggaataagc tcccggcagt
     8161 cgtccattat gaagacccgg cggatgtaga gctggatccc cctgaagaca tcccttatga
     8221 agatacccag gggtgccttg gagggaatga aaagcagcgc ccggaactcc atcccgccct
     8281 cggccttgaa gaagatcctc ctggccggtt tgttccagtc gtgagaaatg tgtctgtaga
     8341 attcttcgta ctcctcctcg ctgacctccc cctccggcct ggtccagatg gctttcatgg
     8401 agttgaggtt ctccagggac ccgtccgcgg acttcatacg tatgggccag gccacaaagt
     8461 cggagtagcg acgaattatc tcccttacca ccgaaggatc tgagtagtcc cttgaggtct
     8521 catccccatt gatcttcttg aggaagaggg taatggtggt cccctggccc tcccgtgaaa
     8581 cctcctcgag tgtgtaggtc ccgtcgccgg gggactccca cttccaggcc gttccttcgt
     8641 cggcccggcg agtgaccagg ctgacccttt cggccaccat gaaagaggag tagaagccta
     8701 tcccgaactg accgatcagc tcctcgggga atgaaccttc acccctttcg cgaaggaccc
     8761 tgacatactc cttcgtgccg gaacgggcta tggtgccaat gaaatccacc agttccgacc
     8821 ggttcatccc gatcccgttg tcggagatgg tcagggtgcc ggctccgggg tcagtctcga
     8881 taataatgtg aaggtcttcg gcaagggggg cgaggttctc atcggtcagc gcttcgaagc
     8941 ggagtttgtc cagtgcgtcc gatgcgttgg agacaagttc cctgaggaag atgtcgcggt
     9001 tggagtagac tgagtggatc atcaggtcca gaagctgtct cgcctcggcc tggaattggt
     9061 aggtctcttt atcgggcatt gattggacct ccttgagatg agtttaaacc tttgtcggtg
     9121 cctgtgatct gcactcatta ataaaggggc ggcgggaaag ccgccgcccc tttatcttaa
     9181 cctatggaga tgtcaaggat cccgacaaaa agggttccag ttactcttgc ctgagttcca
     9241 gaacccgccc ctggacggcc ctgctcttgg gaagcctgac ggtgatcacg ccgtttttga
     9301 agctggcctg agcactttcg gggtcgacct cggtcggaag ggtcaagctg aggttcatgg
     9361 tcctgttgcc ccttctcatg taccaggtcc tgccgtcatt ctcaccctcg gagctctcct
     9421 cgacattgga acgaacgttc accctgtcgg agaaagtctt gatctcgatc tgtgaagggt
     9481 caaaaccggg agcatcgatg ttcaccacga cctcgttgtt ctcctcgtac atatcgacgg
     9541 gaacgtatct ccgggtcata ggcctgatgg catccgagaa acccctgaaa acatcgtcca
     9601 tctcatcgaa tatgcttgtg cctctaccga acgggaccat ctgtctcatg aaaaaccccc
     9661 tccttgtcag ttgttttcga aggaaggata gcaattttcg tcagtaaagg tcaaattatt
     9721 tggtcaggaa aggttagtgt tctttgccgg attctgggaa atatttctga taatagctgt
     9781 ttttctctct gtttttcgtt ttttaagctg gggatctacg aacagatccg gacagggagc
     9841 ggctcccgcc gccccctgtt gtttctggtc ttgttgtttc tggtcttgtt gtttctggtc
     9901 ttgttgtttc tggtcttgtt gtctctggtc taactgttgc ttttcccgtt caccgtttga
     9961 cgcctattgg ttttgaagcc tttccctctc acttctcaca cacttgatcc tgcgattcat
    10021 gatttacggg ttcttctgcc cttcctgccg cgggctattc tttcaaggac tcttccacgg
    10081 ctgccaggga gtgcaggcgg gtggtgtcga agaccgggac atgcgtatcg gactgcctga
    10141 ccagcagggg gatctcggtg cagccgagga caacaccctg gacaccccgg gatgcaagac
    10201 cgtcaattat ccccacgaaa cgttcccggg aggtttcctt gatgttccca aaggtcagtt
    10261 cttcgtctat caccctgttg acgaagtccc tgtcttcctt ctccggcacc aggacttcca
    10321 gaccgtgcag gtcggcaagg cggcccttgt agaaatcctg ctccatggtg aagatagtgc
    10381 ccagcaagcc cactttgctg aaaccctgga cctttacggc agaggccgtc acgtccacca
    10441 ggtgaagcag gggtatcgat accgcctccg aggcgaagtt cgcgaccttg tggatggtgt
    10501 tggaacatat aagaaggaaa tcggccccct ggcgctcaag acccgtggcc gcttcggcca
    10561 ccctggcccc gatctcatcc cagcgccctt ccagcatggg agtgtcgata tcctgaaagt
    10621 cgaagctgtg cataaggatc cgggctgaat gtactccacc cagcctccgg gcgacctcct
    10681 cgtttattat cctgtagtac tcgatggtcg actcccaggt caacccgccg atcaggccta
    10741 tggttttcat gggataaccc ccctttttgg acaggttttt tcgttaggcc agttgagaat
    10801 ttgggtgctg tgtctttggt cgcaaaaagt gattcgtaaa tcgtgaatcg aaaaagcgtg
    10861 agaggtgaga cgtaacacgt gagacgaaag ggtaaaatcc aaagggatca acgaccctca
    10921 gtcgcttgct ctccgggcag agcaaaggac gccctcctgg gctttctctg ctcatccgcg
    10981 acggctcagc cgctcaccgg ctcctttgtt ccggtaaaga tcccgccagt tcattccttt
    11041 tctttcacac ttcacccttc aaacttgcca tttgatctcg gatcaaacag tttcctcacc
    11101 ccgccggctg gcgggttcgg aatgagaaga ccttgtttcg cttttcgggt ttccggcttt
    11161 cctgaggcct gacgcctgcc tcctgtcgcc tggtttattg accttcccaa gtcaggcgac
    11221 acaagtcacg agttacgagt cacgagttac gctcttcaat atccccctgg cggcgacgat
    11281 gccgctggcc gaggcctgga tgacgcccct tgtgacccct gcgccgtcgc cggaggcgaa
    11341 gagtcccggg atgttcgtca tgaggtccct gtcgagagca agcttcaggc tgtagaactt
    11401 tacctcaacc ccgtagagca gggtgtgctt gccattgatg ccgggcatga tc
//
LOCUS       DAOPKQ010000017        11427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_5942_length_11427_cov_4.326064, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000017 DAOPKQ010000000
VERSION     DAOPKQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5942_length_11427_cov_4.326064"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PK146_01260"
     CDS             complement(<1..1269)
                     /locus_tag="PK146_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HPE90895.1"
                     /translation="MFALCDCNNFYASCERLFRPDLERRPVAVLSNNDGCVIARSSEV
                     KALGVPMGAPYFREEGRFRHFGVEVFSSNYPLYEDLSRRVTDSLRSLVPDVEVYSIDE
                     AFLYMDTLPEAAADPSEFCRRVRERVLHWTGIPVSIGLGETKTLAKAANRIAKKDPSR
                     EGIFTMPHGEEADRYLEGIDIGDVWGVGFRNAPKLRRLGVRTARALKHADGEWIRRRF
                     TICEARTSRELNGLVCFPFTEAPGPRKTIRVSRSFGDPVTELLSLREAISYFAFSAAE
                     TLRGEGSAAGKIHLFLMTSPFGSAPRYANAATARFDVPTSCTTDIVGQALKCLERIFV
                     EGYPYKKAGVALTDLADGAHLQGHLFREIKKPWEGSLMKAVDHLNMEFGRGTVYTASM
                     GIRDRRSWVMRRGHVSPRYTTCWEDLPSVLP"
     gene            complement(1354..1872)
                     /locus_tag="PK146_01265"
     CDS             complement(1354..1872)
                     /locus_tag="PK146_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90896.1"
                     /translation="MNTMNNTIKRCWTIVAVLVFLHTAAFGAVTLQEWNSGDVGLLMT
                     RQDVLNAIGAPDGSEGTTEWYDISGNEGLSRVTLDYLGEERVQAVHLAFRTGSVDLET
                     LSSIVRSAFPGIPEVHSDDRMAIFLGRSGDTGEPVYFLALVEDPQKGSGPELISMTES
                     ANLHYQGGQNQE"
     gene            2000..2203
                     /locus_tag="PK146_01270"
     CDS             2000..2203
                     /locus_tag="PK146_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90897.1"
                     /translation="MVRSQITCPYCGSSETVPVVYGYAPFDLLLRARRGELVLGGVCV
                     EPGQPQWKCLACDRLIPLEEVPN"
     gene            2200..2673
                     /locus_tag="PK146_01275"
     CDS             2200..2673
                     /locus_tag="PK146_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPE90898.1"
                     /translation="MKKYAPLALFVLLCLGAGALGSIATASSVSTWYPGLLKPSWKPP
                     SWLFGPVWTLLYVLMGSSAWRVWLTAGGFSDAREEMILFFVQLTLNVAWSWIFFGLRM
                     PGAALLELVLLWVAIAVTIRAFARMDRIAAWLMVPYIAWVTYAGALNASIWFLNR"
     gene            2832..3326
                     /locus_tag="PK146_01280"
     CDS             2832..3326
                     /locus_tag="PK146_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPE90899.1"
                     /translation="MVIFVLSVATYLLLAWSGGGIDPIEVGIAVALGGVMAGLARAWN
                     PGRSWTLAGLNPLRWIAFVIYLALPFLWGMIKANFDVAFRVVTGKIRPGIVRIDSGLK
                     GSLAQAILANSITLTPGTLTVDVDDSGETGVFYVHWINVTDEDPSEKKVYGSFGKWAR
                     RVAE"
     gene            3323..3583
                     /locus_tag="PK146_01285"
     CDS             3323..3583
                     /locus_tag="PK146_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPE90900.1"
                     /translation="MMITYFAWAAMITGICALAMVGRLVAGPTIPDRIVALDSINTMV
                     VALMILLSLVYDSVVMVDVAIVYAALSFVGTMFIARYIEGGV"
     gene            3586..3960
                     /gene="mnhG"
                     /locus_tag="PK146_01290"
     CDS             3586..3960
                     /gene="mnhG"
                     /locus_tag="PK146_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPE90901.1"
                     /translation="MEKAALILLSVGVVFNFLGTVALYRFPDVYTRLHGTTKCTTFGS
                     IFTSAAVVVFALDMFFRTGEGRFNTLTVHVIIAILALLISNATGAHAIARAAHRSGVL
                     PKYAVVDRLAEDHRKKKGGVSA"
     gene            3957..4220
                     /locus_tag="PK146_01295"
     CDS             3957..4220
                     /locus_tag="PK146_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPE90902.1"
                     /translation="MNGYAHMAVLTIIVISAFYAVWFRDLLSSVISLAVFSLLLSLEF
                     FILQAPDVAIAEAAIGAGLSTAIYIIALRACKTLRKPNGGEGR"
     gene            4217..4492
                     /locus_tag="PK146_01300"
     CDS             4217..4492
                     /locus_tag="PK146_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90903.1"
                     /translation="MKRRVLFLVAVLVVAGVFWGALSRIHPFGDTARAPMDDYYLENA
                     QQERSVNNVVTSIVFDYRGFDTLGEAAVLFTAVCSVLALFRKGSEGK"
     gene            4489..4986
                     /locus_tag="PK146_01305"
     CDS             4489..4986
                     /locus_tag="PK146_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPE90904.1"
                     /translation="MNPLSVVVRTICDIFAWFMGIFGAYIIIHGHLTPGGGFQGGAVV
                     ATMAAFLLVAYGGKKVLSFVRPGFYSGMETAGLLLFIAAGFLGMGTTFFYNFLAGRGG
                     LFGAVVPIGPSSGILNSAGTISVMNIAVGLEVIGGLSLILIYMFKGIRLFDDHAGRSE
                     SGHDR"
     gene            4976..5323
                     /locus_tag="PK146_01310"
     CDS             4976..5323
                     /locus_tag="PK146_01310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HPE90905.1"
                     /translation="MTGNAPFLVVGIIFFLGMYAILFERNLIKIAIGITLLEASANLL
                     LIVLGFRTGGTIPIFTLAGGTRTMVLPTPQALTLTAIVIGLATSALLLSLVILLYRHY
                     GTLDVREIRRLRG"
     gene            5320..6885
                     /locus_tag="PK146_01315"
     CDS             5320..6885
                     /locus_tag="PK146_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPE90906.1"
                     /translation="MNWQIHLPALALAVPLLGAFVSPVAGLGGRLTRNAWMVLVSMST
                     IAVCILLWQRVIAGGTVVYVMGAEAWNITLPSGLSWPIRIILEVDAFSAFMAVVGSIA
                     ALAGAFFSLRFMDKFTGLEKFSALFFLLVTGMLGMQLTGDLFNFFVFLEIASVASFGL
                     IAFWRDRPEAVEASFKYMVVSTISAMFVLIAVGFFYGRYNAVNIAAVARMLHMGYAEK
                     IALVFIVVSLAMKCGAVPMHMWTPDAYAEAPAGVTCLLVAVSQASLYGLFRVCFSLYG
                     VSLGSTVVPWTIIVMGVLSMFIGVTMAVIQKEMKRLMAYHSVSQIGYILMALGVGLLS
                     LKDPQAMADYGFTAMKGGIFHIINYTMYKGLLFLTAGAVFYSAGTRNLNELGGLARLM
                     PQTTFMFVIAAAAIAGLPPFNGFVSKLLIYQSTFAVYPLLAVAALVTSVLTLASFVKV
                     FQTAFLGPAKKTFEKVTEVPASMLLGMSVLTIVIVALTLFPGWTLSHLVEPAAKALVD
                     QAGYISAVMGGGL"
     gene            6885..7223
                     /locus_tag="PK146_01320"
     CDS             6885..7223
                     /locus_tag="PK146_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPE90907.1"
                     /translation="MWEKVYSGFGYWDVYLWLLFFVIAAGMILFIRALGRSDFKEGTE
                     QDEIFYGSNPVPEDGADMGVPAASAYWGFVEALRPYYDWLVELHTGIASDYVGYFVLT
                     VAAVAVLVLL"
     gene            7236..7697
                     /locus_tag="PK146_01325"
     CDS             7236..7697
                     /locus_tag="PK146_01325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPE90908.1"
                     /translation="MKLERYLDILTKSIWVFHCNSGSCNGCDIEIVATITPRYDIERF
                     GMKLVGTPRHADALLVTGPVTKYMTGRLRRIYAQMPDPKVVIAIGNCACSGDVFYKSY
                     NLDGPVDNIIPVDVYVHGCPPRPEAIIEGAAKAVLKLEKLKDSLKKAGAAK"
     gene            7694..8245
                     /locus_tag="PK146_01330"
     CDS             7694..8245
                     /locus_tag="PK146_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPE90909.1"
                     /translation="MKPRCAEYTEEYVSPDKVLSLLRRKFRKTADSMELRDYKGGAGN
                     ETAYQCLWVQVEKQRFLDLVDALREIDFLHFQITSGDDIGEAVQLNYHFSIFRCAGRG
                     KRLGVTVTVDVPKDDLVMPSLWSRIPGIEYSEREIREMFGVDFDGLPNKALVFLPEDW
                     NEDIKPWRRDETGPRPEDIRELS"
     gene            8257..9495
                     /locus_tag="PK146_01335"
     CDS             8257..9495
                     /locus_tag="PK146_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPE90910.1"
                     /translation="MAEGLNKTYKVPIGPVHVGLKEPITAWLDVDGERIKGATIRPGA
                     IHRGIEFMARDRNPIQIIYLAERICGICSFAHIVAFVRAVEDAARIEVPARAQYIRTI
                     VLELERIHSHILWAGVACYTFGYDSAFNLGMILREKVMDVLEALTGNRVNYGTGTVGG
                     VRRDITPAVGKVIREMIDFYKNEFGVFYDVVTDDPIAKARMRDVGVLSKEDAIAYCAL
                     GPTVRGSGLRVDLRWSSPYEAYCDMDIKPVVPQDYTGEVYGDVFDRFLVRVLEVYQSL
                     EIIENAMAGLPSGDLMWEPKLPKLLNYLKQADGTGLSSIEAPRGDDTHAIRLTGGQDN
                     ITWWKVRAPTYSNAVSWPIMFMDNELADSPLIINSIDPCISCMERMVVTDRSTGSGNI
                     VTKSELIERCRDKTRRMMGS"
     gene            9492..10484
                     /locus_tag="PK146_01340"
     CDS             9492..10484
                     /locus_tag="PK146_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPE90911.1"
                     /translation="MTLLHFKLIAGVALLFFASLLSLVFEGVDRIIHARMQLRLGPPL
                     FQPFFDILKLLGKENIVPRRAIPWAFNGAPWVSATAALMILLYIPMGSLPPILAGSGD
                     LILILYLLGLSAVAMAVGGFASGSTYANVGAQREMTLMMSYELPLAVVIVSLAWFAYR
                     TGMPGEPFSLETFVGMPLWSVVGWTGVFGLLCLLVALMAVVPAEVGKVPMDIAEAKTE
                     ILEGLICEYSGRNLAMFKIAFSLRTLVMCAVVVSLFFPWTIGRHFGLQGSLLFLADFI
                     FFWVKVFLMQIIGVTTVRTAFGRYKIWQASQFYWFHIAGLSLAGMVLLSLDVVL"
     gene            10497..11132
                     /locus_tag="PK146_01345"
     CDS             10497..11132
                     /locus_tag="PK146_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE90912.1"
                     /translation="MINRMMIQIFRQWCEDPATNPFPVAHMPDNLTEALKAAAEGKIK
                     LNDPVKTPEGFRGRIGYDKKTCIGCKMCIKVCPANAIESLDEEKKVQFHMDRCCFCAQ
                     CTEICPVSCIWMTDEFAFSSYERKKQIVKDSGKRPAAKKVKGAPEANATEATKYHIDP
                     DKCIGCTRCSKVCPVQAISGKIKEKHVIDEEKCIGCSACAEACPVKAISPR"
     gene            11195..>11427
                     /locus_tag="PK146_01350"
     CDS             11195..>11427
                     /locus_tag="PK146_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPE90913.1"
                     /translation="MGKVQVIFTGGTIASGFLGERKGPDPSASKALHESISSFFAEKD
                     MEVECIEPWGIPGLDNSNLDPGHWIEMTRAIDEA"
BASE COUNT         2163 a         3344 c         3411 g         2509 t
ORIGIN      
        1 gggcagcacg gaggggaggt cctcccagca ggtggtgtag cggggcgaga cgtgccccct
       61 gcgcatcacc caggaccttc tgtccctgat gcccatcgag gcggtgtaga ccgtccccct
      121 gccgaactcc atgttaagat ggtccactgc tttcattagc gatccttccc agggtttttt
      181 aatctccctg aagaggtgtc cctgcaggtg agccccgtcg gccaggtctg taagcgccac
      241 tccggccttt ttataggggt agccttccac gaagatcctc tccaggcact tgagtgcctg
      301 accgacgatg tcggtcgtac aggaggtggg aacgtcaaat cttgccgtgg ccgcgttggc
      361 gtaacgcggg gcgctgccga aggggctggt cataagaaaa aggtgtatct ttccggcggc
      421 cgaaccctcc cccctgaggg tctccgccgc tgaaaaggca aagtacgaga tggcctccct
      481 cagggagaga agttccgtca ccggatcgcc gaagcttctg gatactctta tggtcttgcg
      541 aggcccgggt gcttccgtga aggggaaaca gaccaggccg ttgagctccc tggaggttct
      601 tgcctcgcag atggtgaagc gtctcctgat ccactccccg tcagcgtgct tgagggccct
      661 ggcagtcctg acccccaggc ggcgcagctt tggggcgttg cggaaaccaa cgccccagac
      721 atcacctata tctatcccct ccaggtaccg gtcggcctcc tcgccgtggg gcatggtgaa
      781 gatcccctcc ctggatggat ctttcttggc gatcctgttg gccgccttgg caagggtctt
      841 cgtctccccg aggcctatgg aaacgggtat gcccgtccaa tgcaggaccc tctcgcggac
      901 ccggcggcag aattccgacg ggtccgcagc ggcctccggc agggtgtcca tgtagaggaa
      961 ggcctcgtcg atggaataga cctccacgtc gggtaccagg ctgcggaggg aatcggtgac
     1021 ccttctggac agatcctcgt agagggggta gttggaggag aagacctcca cgccgaaatg
     1081 cctgaacctg ccctcctccc tgaaataggg agcacccatg ggaacaccca gggccttgac
     1141 ctccgaggac cgggctatga cgcatccgtc gttgttggag aggaccgcca cggggcggcg
     1201 ttcaagatcg ggcctgaaga gcctctcgca ggaggcgtag aagttgttgc agtcgcacag
     1261 ggcgaacatg atcccacact cccggaagga atgctcccta aagattactg taaggataca
     1321 ttacccggga aataattaca agtatctatc tggttattcc tggttctgtc ctccctgata
     1381 gtgaaggttg gccgactccg tcatggagat cagttcgggg ccgcttccct tctggggatc
     1441 ttcgaccagg gccaggaagt agaccggttc accggtatcg ccggacctcc ccaggaagat
     1501 cgccatacgg tcgtcgctgt ggacttcggg tatgcccgga aaggcactcc tcacgatgga
     1561 ggaaagggtc tccaggtcaa cagagcccgt cctgaaggcc aggtgtacag cctgtaccct
     1621 ctcctccccg agatagtcca gggtgaccct tgagagtccc tcgttccctg agatgtcgta
     1681 ccattctgtg gttccctcgg aaccatcggg cgcccctatg gcgttcaata catcctgcct
     1741 ggtcatgagg agccctacat ccccggagtt ccactcctgg agggtgaccg ctccgaaggc
     1801 ggccgtatgg agaaagacaa gaacggcaac tatggtccag catcttttga tcgtattgtt
     1861 catggtgttc atttcctttc ctctggcaat agggttacaa taaattccag gtgttgccga
     1921 ccctgttgtt tttctgatca agactaacac gccatggcag atcccgaaag cgtaaaaatg
     1981 ccgggaggtt cttccataga tggtccggtc ccagatcacc tgcccctact gtggttcatc
     2041 cgaaacggtc ccggtagtat acggctatgc gcccttcgac ctcctcctcc gcgccaggcg
     2101 cggggagctg gtcctgggag gggtctgcgt ggagccgggg cagcctcagt ggaagtgcct
     2161 ggcctgcgac agactgatcc ctttggagga ggtgccgaat tgaagaagta tgcccctttg
     2221 gcccttttcg tgcttctgtg cctcggtgcc ggggccctgg gttcgatagc cacggcgtct
     2281 tccgtttcga catggtatcc cgggttgctc aaaccttcct ggaagccgcc gtcatggctc
     2341 ttcggtccag tctggacgct cctctacgtg cttatgggtt catccgcctg gagggtctgg
     2401 ctcacggcag gagggttcag tgacgcccgg gaggagatga tcctcttttt tgttcagctg
     2461 accctcaacg tggcctggtc ctggatcttc ttcggtctca ggatgcccgg ggctgccctg
     2521 ctggagctgg tccttctctg ggttgccata gctgtcacta tcagggcctt tgcccgaatg
     2581 gaccggatcg ctgcctggtt gatggtcccc tatatagcgt gggtcaccta cgcaggggct
     2641 ctgaacgctt caatatggtt tttgaacagg tagtccatac cggaatttat tatgtataat
     2701 gcataattgg atttcttcag cccccctcct gcaggtgcca ggagagggcg ccaaatgggt
     2761 atcaaggtcc gagcggactt tttttgttct gttccagtgg ttctgtacac aggaaatagg
     2821 aggtgaggcg tgtggttatt tttgtcctct ccgttgccac gtacctcctg ctggcgtgga
     2881 gtggaggagg aatcgacccc atcgaggtgg ggatcgcagt tgccctgggg ggcgttatgg
     2941 ccggactggc ccgggcctgg aacccgggga ggtcgtggac ccttgcggga ctgaacccgc
     3001 tgcgctggat cgcttttgtt atctatcttg cgttgccctt cctgtggggc atgatcaagg
     3061 ccaacttcga cgttgctttc agggtggtca ccgggaagat acggcccggg atcgtcagga
     3121 tcgactccgg acttaaggga agcctcgccc aggcgatcct tgccaactcc atcaccctga
     3181 cgccgggcac gctgacggtg gacgtggacg actccggaga gacgggggtt ttctacgtgc
     3241 actggatcaa cgtgaccgac gaggacccgt cagagaagaa agtctacggt tccttcggga
     3301 agtgggcaag gagggtcgcc gaatgatgat cacctatttc gcatgggcgg ccatgatcac
     3361 cgggatctgt gccctggcca tggtgggaag gcttgtcgcg ggcccaacca tccctgacag
     3421 gatcgtagcc ctcgactcca taaacaccat ggtggtggct cttatgatcc ttctttcgct
     3481 ggtgtacgac tcggtggtca tggtcgatgt ggccatcgtc tacgcagcgc tctccttcgt
     3541 gggcaccatg ttcatcgccc gctacataga ggggggtgtg tagtcttgga gaaagcagcc
     3601 cttatccttc tctcggtcgg tgtcgtgttc aacttcctcg gaacggttgc cctgtaccgc
     3661 ttccccgatg tctacacccg tcttcacggc acgacgaagt gcactacctt cgggtccata
     3721 ttcacctcgg cggctgtcgt tgtcttcgcc ctggacatgt tcttccggac cggtgaaggt
     3781 cgtttcaaca ccctgacggt ccacgtgatc attgccatcc tggcacttct gatatccaat
     3841 gccaccgggg cccacgccat cgcgcgggcg gcccacagga gcggcgtact tcccaagtat
     3901 gccgtagtcg acaggcttgc cgaagaccat cggaagaaga aaggcggtgt ttcggcatga
     3961 acggttacgc ccacatggcc gtgctgacga tcatagtcat ctcggccttc tacgccgtat
     4021 ggtttcgtga cctcctctca tcggtcatct cactggctgt cttcagtctc ctgctctccc
     4081 ttgagttctt cattctccag gctcccgacg tggccattgc cgaagcggcc atcggagccg
     4141 ggctgagcac ggccatttac attatcgccc tccgggcctg caagaccctg aggaaaccga
     4201 acgggggtga ggggcgatga aaaggcgcgt ccttttcctt gttgcggttc tggtagttgc
     4261 cggtgtcttc tggggcgccc tgagcaggat ccaccccttt ggtgacaccg ccagggctcc
     4321 catggacgac tactatctcg agaacgccca gcaggagcga tccgtcaaca acgtcgtaac
     4381 ttccatagtc tttgactaca ggggattcga caccctgggc gaggcggcgg tgctcttcac
     4441 cgcggtctgc tcggttctgg cccttttccg gaaggggagt gagggaaaat gaatcctctt
     4501 tccgttgtgg taaggaccat ctgtgacatc tttgcctggt tcatgggcat attcggtgcc
     4561 tacatcataa tccacgggca ccttaccccc ggcgggggct tccagggcgg ggcagttgtc
     4621 gctaccatgg cagccttcct tcttgtcgcc tacggaggga aaaaggtgct ctccttcgtg
     4681 aggccgggat tctactcggg gatggagacg gcggggctgt tgctcttcat agccgccggg
     4741 ttcctcggta tgggcacgac cttcttctac aacttcctgg ctggaagggg cggcctcttc
     4801 ggagctgtcg ttcccatcgg tcccagcagc ggcatcctca acagcgccgg caccatatcg
     4861 gtgatgaaca ttgccgtggg actcgaggtc ataggcgggc tctcattgat cctgatctac
     4921 atgttcaagg ggatccgtct cttcgacgat catgcgggaa ggagtgagag cggccatgac
     4981 aggtaacgcc cccttccttg tggttgggat catatttttc ctggggatgt acgccatcct
     5041 cttcgagaga aacctcatca agatagccat aggcataacg ctcctggaag cgtcggccaa
     5101 cttgctgctt attgttctgg ggttccgtac gggtggcaca atacctattt ttaccctggc
     5161 aggcgggacc aggaccatgg tcctccccac cccccaggcg ctcacgctga cggctatcgt
     5221 cataggtctg gcaacttcgg ccctgctcct ttcgctggtc attcttctgt acagacacta
     5281 cgggacgctt gacgtccgcg agatcaggag gttaagggga tgaactggca gatacaccta
     5341 ccggcgcttg ccctggccgt ccccctcctg ggagcctttg tctctccggt ggccggtctc
     5401 ggcggacgct tgacgcgcaa cgcatggatg gtgctcgtct ccatgtcgac catcgccgtc
     5461 tgcattcttc tatggcagag ggtaatagcg ggcgggaccg tcgtctacgt gatgggtgcc
     5521 gaggcgtgga acataacgct tccctcgggg ctcagctggc ccataaggat catcctcgag
     5581 gtcgacgcct tcagtgcctt catggcggtg gtgggatcca tagccgccct cgcgggggcc
     5641 ttcttctctc tacgcttcat ggacaagttc acaggcctcg agaagttctc ggccctcttc
     5701 ttcctgctcg tcacggggat gctgggcatg cagctcaccg gggacctctt caacttcttc
     5761 gtcttcctcg agatcgcctc ggtggcctcc ttcgggctta tcgccttctg gagggacaga
     5821 cctgaagcgg tggaagcctc cttcaagtac atggtggtct ccaccatctc ggcaatgttc
     5881 gtacttatcg cggtaggatt cttctacggg cgctacaacg ctgtcaacat agcggccgtc
     5941 gccaggatgc tccatatggg ttacgccgag aagatagccc tggtgttcat tgtggtctcc
     6001 ctggcgatga agtgcggagc agtccccatg cacatgtgga cccccgacgc ctacgccgag
     6061 gcgccagcag gggtgacctg cctcctggtt gccgtaagcc aggcgtcgct ctacggcctc
     6121 ttccgggtct gcttctccct ctacggggtc agcctgggga gcaccgttgt cccctggacg
     6181 ataatcgtca tgggtgtcct ttccatgttc atcggggtca caatggccgt catacagaaa
     6241 gagatgaaac ggctcatggc ctaccattcg gtctcccaga taggctacat actgatggcc
     6301 ctcggagtag gtctgctctc cctgaaggat ccccaggcca tggctgatta cggttttacg
     6361 gcaatgaagg gcggcatctt tcatatcatc aactacacca tgtacaaggg gctgctcttc
     6421 cttaccgccg gtgcggtgtt ctactcggct ggcacccgta acctcaacga gctcgggggt
     6481 ctggccaggt tgatgcccca gaccaccttc atgttcgtca tagcggcggc ggccatcgcg
     6541 ggcctgccgc ccttcaacgg tttcgtctcc aaactgctga tctaccagtc gaccttcgcc
     6601 gtctacccgc tgctagcggt ggcggccctg gtcacttcgg tgctgacgct ggcctccttc
     6661 gtaaaggtct ttcagacggc cttccttggt ccggcaaaga agactttcga gaaggtcacc
     6721 gaggtgcctg cgtcgatgct cctggggatg tcagtgctta caatagtcat tgtggccctg
     6781 acgctcttcc cgggatggac gctctctcac ctggtggaac ctgccgccaa ggccctggtt
     6841 gaccaggcag gctacatttc cgccgttatg ggaggtggtc tgtgatgtgg gaaaaggtct
     6901 actcgggatt cggctactgg gacgtatact tatggctgct cttcttcgtc atagcagcgg
     6961 gcatgatcct gttcataagg gctcttggcc gcagcgactt caaggagggc accgagcagg
     7021 acgaaatatt ctacggcagc aacccggtac ccgaggacgg cgccgatatg ggggttccag
     7081 ctgcctcggc ctactggggg ttcgtggagg ccctcagacc ctactatgac tggctggttg
     7141 aactccacac cggcattgcg agcgactacg tgggctactt cgttctcacc gtggcggctg
     7201 tcgcagtact ggtccttctg taagggggtg ggacgatgaa acttgaacgc tacctcgaca
     7261 ttctgaccaa gtccatctgg gtctttcact gtaacagcgg gtcttgcaac ggctgcgaca
     7321 tagagatcgt ggcgaccatt acccccaggt acgacataga gcgtttcgga atgaagcttg
     7381 tggggacccc gcgccacgct gacgcgctgc tcgtaacggg acccgtaacg aagtacatga
     7441 cgggcaggct ccgcaggatc tacgcccaga tgcccgaccc caaggtggtc atcgccatag
     7501 gcaactgcgc ctgctcgggc gacgtattct acaagtctta caacctggac ggcccggtgg
     7561 acaacataat ccccgtcgac gtctacgtcc acgggtgccc tcccaggccc gaggccatca
     7621 tagagggcgc cgccaaggcg gttctgaagc tggagaagct caaggactcc ctcaagaagg
     7681 caggtgccgc gaaatgaagc cgcgctgtgc agagtacacc gaagagtacg tgagccccga
     7741 taaggtgctc tcgcttctca gaagaaagtt ccgcaagact gccgactcca tggagctcag
     7801 ggactacaag ggaggtgccg gtaacgagac ggcctaccag tgcctctggg tgcaggtgga
     7861 gaagcagcgc ttcctggatc tggtggatgc gctgagggag atcgactttc tccactttca
     7921 gatcacctcc ggcgacgaca tcggtgaggc ggtccagctc aactatcact tctcgatctt
     7981 ccgctgcgcc gggcggggca aaaggctggg agtcacggtg accgtcgatg ttccgaagga
     8041 cgatctcgtg atgccatccc tctggagcag gatcccgggg atagagtaca gcgagaggga
     8101 gatccgcgag atgttcggcg tggatttcga cggattgcca aacaaggccc tggtttttct
     8161 ccccgaggac tggaacgagg atatcaagcc ctggcgcagg gacgaaaccg gtcccaggcc
     8221 ggaagacatc agggaactct cctaggaggt gccggtatgg cagaaggatt gaacaaaacg
     8281 tacaaagtgc cgatcggccc tgttcacgtg ggactcaagg aacccattac ggcctggctc
     8341 gatgtcgacg gggagcgcat aaagggtgct accatacgcc ccggggccat tcacaggggc
     8401 atagagttca tggcccgcga ccgcaacccc atacagatca tctaccttgc cgaacggatc
     8461 tgcgggatat gctccttcgc ccacatcgta gccttcgtcc gggccgtgga ggatgccgcc
     8521 aggatagagg tcccggcaag ggcccagtac atcaggacca tcgtgttgga gctggagagg
     8581 atccactctc acatcctatg ggcaggagtt gcctgctaca ccttcggcta cgactcggcc
     8641 ttcaacctcg ggatgatcct gagggagaag gtcatggatg tccttgaggc actaacgggg
     8701 aaccgggtca actacgggac cggaaccgtc ggtggtgtcc gcagggacat aactcctgcg
     8761 gtaggcaagg tcatcaggga aatgatagac ttctacaaga acgagttcgg cgtcttctac
     8821 gatgtcgtca ccgacgaccc catagccaag gccaggatgc gcgacgtggg cgttctctcg
     8881 aaggaggacg ccatagccta ctgcgccctt gggcctacgg tcagggggag cggcctcagg
     8941 gtcgacctgc gttggtcgtc cccctacgag gcctactgcg acatggatat caagcccgta
     9001 gtcccccagg actacaccgg ggaggtctac ggcgacgtct tcgaccgctt ccttgtcagg
     9061 gtactggagg tctaccagtc cctggagata atagagaacg ccatggcagg gctgccgtcg
     9121 ggggacctca tgtgggagcc gaaactaccc aagctcctca actacctcaa gcaggctgac
     9181 ggtacgggcc tgagctccat cgaggcgccc cgcggcgacg acacccatgc gataaggctt
     9241 accgggggcc aggataacat tacctggtgg aaagtccgcg ctccgacata ctccaacgcg
     9301 gtctcgtggc cgatcatgtt catggataac gagctggccg actccccgct gatcatcaac
     9361 agcatcgacc cctgcatttc ctgcatggag cgaatggtcg tcaccgaccg gtcgacagga
     9421 tccgggaata tcgtgacgaa gtcggagctc atcgagcgct gtcgtgacaa gacaaggagg
     9481 atgatgggat catgaccctt cttcatttca agttgatagc gggggtggcc ctcctgttct
     9541 ttgcttccct tctttccctt gtctttgaag gggtggacag gataatccac gcccggatgc
     9601 agctcaggct gggaccaccg ctctttcagc ccttcttcga catactgaag cttcttggca
     9661 aggagaacat tgttccccga agggccattc cgtgggcgtt caacggggct ccctgggtct
     9721 cggccactgc ggcccttatg atacttctct acatacccat gggttcgctg cccccaattc
     9781 tggccgggag cggcgacctt atcctcatcc tctacctgct ggggctctcc gcggtggcga
     9841 tggcagtggg aggttttgca agcggctcca cctacgccaa cgttggcgcc caacgcgaga
     9901 tgactctgat gatgagctac gaacttcccc ttgcggttgt tatcgtctcg ctggcctggt
     9961 tcgcttacag gaccgggatg cccggtgagc ccttcagtct cgagaccttc gtcgggatgc
    10021 cgctgtggag cgtagtgggg tggacgggcg tcttcggcct gctctgcctg ctcgtggccc
    10081 tcatggcggt ggtgcccgcc gaggtgggga aggtgcccat ggatattgcc gaggcgaaga
    10141 cggagatcct ggaagggctc atctgcgagt actccggccg taacctggcc atgttcaaga
    10201 tagccttctc cctgcgaacg ctggttatgt gcgccgtggt ggtatccctc ttcttcccat
    10261 ggaccatagg caggcatttc ggcctccagg gttcgcttct ctttctggcg gacttcatct
    10321 tcttctgggt gaaggtcttc ctgatgcaga taatcggcgt aacgacggta cgtactgcct
    10381 ttgggcggta caagatatgg caggcctcgc agttctactg gttccacatt gcggggctct
    10441 ccctggcggg gatggtcctg ctgtccctcg acgtggtcct ttaagggggg gttggaatga
    10501 taaacaggat gatgatacag atcttcaggc agtggtgcga ggatccggcc accaacccct
    10561 tccccgttgc ccacatgccc gacaacctca ccgaggctct caaggccgct gctgagggca
    10621 agatcaagct caacgacccg gtaaagaccc ccgaggggtt ccgcggcagg atcggctacg
    10681 acaaaaagac ctgcataggg tgcaaaatgt gcatcaaggt ctgcccggcc aacgccatcg
    10741 aatccctcga cgaggagaag aaggtccagt tccacatgga tcgctgttgc ttctgtgccc
    10801 agtgcaccga gatctgtcca gtatcgtgca tatggatgac cgacgagttc gccttctcgt
    10861 cctacgaacg gaagaaacag atagtgaagg actccggaaa gaggcctgcc gccaagaagg
    10921 tcaagggtgc tccggaggcc aatgcgaccg aagcgaccaa gtatcacatt gaccccgaca
    10981 agtgcatcgg ctgcaccagg tgctccaagg tatgccccgt tcaggctatt tctggtaaga
    11041 tcaaagagaa gcacgttatc gacgaggaga agtgtatagg ctgttcggcc tgtgcggagg
    11101 cgtgccctgt aaaggcgata agcccaaggt agggtgaact tgaggggcgg ggtcgcggaa
    11161 gttcgcggtc ccgccatttt ttcggaggtg acccatggga aaggttcaag tcatcttcac
    11221 cggaggcacc attgccagcg ggttcctggg agagaggaag ggacccgacc cttcggcctc
    11281 gaaggcactg cacgaaagca tatcgtcctt cttcgccgag aaggacatgg aagtggagtg
    11341 catagagcca tggggtatcc ccggccttga caactctaac ctcgaccccg gccactggat
    11401 agagatgacc cgggcaatag acgaggc
//
LOCUS       DAOPKQ010000018        11049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_6217_length_11049_cov_5.980444, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000018 DAOPKQ010000000
VERSION     DAOPKQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6217_length_11049_cov_5.980444"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..813)
                     /locus_tag="PK146_01355"
     CDS             complement(322..813)
                     /locus_tag="PK146_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPE90914.1"
                     /translation="MKITGKVWKYGDDVNTDVIFPGKYTYTVSDRAEMGKYALEDLDP
                     EFTRNAQAGDIIVGGKNWGCGSSREQAVTCLKVRGIGAIIAKGFARIYYRNALNEGLP
                     IVVSPEAVDALEKGQEVTIDFDRSEIVTPKGTFKFPPYPDFVKGLIEDGGLIPHVKKS
                     LGL"
     gene            complement(818..2074)
                     /locus_tag="PK146_01360"
     CDS             complement(818..2074)
                     /locus_tag="PK146_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPE90915.1"
                     /translation="MGKTFAEKVLGKAAGESVSAGQVVIVEPHFCMSHDNAAPIWGTF
                     KKIGVDKVWKPDHLVFILDHAIPAPTDKHAENHKQIREVVKEQGIRYFYDVTSKGGVC
                     HQIMCEEGFALPGLVMVGSDSHTCTYGAFGAFSTGIGRSEMAATWATGQIWFRVPESM
                     KITVKGKFNKGVSAKDLILKIAGDIKADGADYMSIEFHGPAIEEMSLAERMTLCNMGI
                     EIGAKNAVCPPDQKVLDFIKPIAKTDEWEAMWADEDAVYAQELNYDLGDIEPCVAKPH
                     TVDNYAPIGEVKGTPIHQAFLGSCTNARIEDLRLAADILKDKEVAVRTIVIPASWSVF
                     RQAMKEGLIDVFLDAGCVITNPGCGPCMGNHEGILAPGETCISTANRNFKGRMGNKES
                     NIYLASPMTVAASALKGEISDPREVL"
     gene            complement(2090..2617)
                     /gene="leuD"
                     /locus_tag="PK146_01365"
     CDS             complement(2090..2617)
                     /gene="leuD"
                     /locus_tag="PK146_01365"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301535.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPE90916.1"
                     /translation="MENKVLRGKAWVFGDDIDTDLIYHNKYLAETDPRKMPQYSFEYL
                     PGKENFAKEVKEGDFVVAGTNFGCGSSREHAVYCLKYAGVPVILAESFSRIYYRNAIN
                     NGYPVLFVKGLSQAIKDGKIKDGDDLEVDLEKGEIRDLTNGNSFHGDAVADLEMDIMK
                     AGGLIEYLKEKAASK"
     gene            complement(2632..3912)
                     /locus_tag="PK146_01370"
     CDS             complement(2632..3912)
                     /locus_tag="PK146_01370"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPE90917.1"
                     /translation="MGKTMIEKIIRRAAGKEVKVGERVWCNIDLSTARDFGGANCVLQ
                     FEEVTDKKGKVWDPDKVAFTFDLQAPSHSEKVSTNQKIIREFAKKQGIKKVFDVDWGI
                     GQHVLLENGLVKPGDVILGTDSHMNLLGAVGAFATGVGNTDIVASWFEGTNWFRVPET
                     MKITVNGKFAKGVCMRDLLTHIVGTLGADGMFFLSTEFYGDTIERSSLSDRITLCSMV
                     TEMSGKIGLIMPNGKVLDWLVERAGKEVVDRVKSLSADPDAVYCQELEFNVDNLEPMT
                     SCPDAPDNVKKVREVAGEHVDQVHIGSCSNGRFEDISAAFEVLKASKSKVAPGMRAII
                     TPATREVMLQCAEAGYIQKFLEAGVIFTNPTCALCTAEHYGALPSGDVGMSTTNRNFI
                     GKVGKGSHTYLASPMTAMATAIRGKITDPRDILG"
     gene            complement(3946..4500)
                     /locus_tag="PK146_01375"
     CDS             complement(3946..4500)
                     /locus_tag="PK146_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE90918.1"
                     /translation="MRDRIVKMIPEIDWIQDKELREKVVATIEDALKTGGWEPEDMDK
                     IPFTLLIPDCPASLNVHNRGVTRMAKVIYEEYNPLYKDNGGFELDHDTLIAGAILHDV
                     GKLVEYEKKDGKAVKSQMGKDLRHPFSGVGLAMRNGIPSRICHCIAVHAKEGDGGYRC
                     PEAVVINKCDFINFETIKSFLGLL"
     gene            complement(4547..5245)
                     /locus_tag="PK146_01380"
     CDS             complement(4547..5245)
                     /locus_tag="PK146_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPE90919.1"
                     /translation="MIEKRLRHTRIYEQVVNELKELIASGELLPGDPLPPERQLMSEM
                     GVSRSSLREAFRVMELLGLIDSVPGKGRFVRKPRGETADSRNMPLEDEAVLELMEARR
                     ALDPAIAGAAAMHAMPSDLMKLRKVLSRTEKNLEHIEKRSQDDFDFHLMLAEATHNFV
                     FINIVKMTFNLIMATHERIYSLLADKEAFFNEHQTIYEAIIDHDVARAQSLSAGHIER
                     IYRTLQEGLALEVK"
     gene            5632..6642
                     /locus_tag="PK146_01385"
     CDS             5632..6642
                     /locus_tag="PK146_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE90920.1"
                     /translation="MKAGRKTFLILALALVVGLTSIPAASAAGNVVMVDFSWSSVQMH
                     NRVAGFILEHGYGFSPDYMFAESVPGLTGLQQGDIDVAMEMWIDNVLEWYTDAVEENK
                     QVLDLGSVFPDAPQGWYVPTYMIKGDKERGIEPMAPNLKSVFDLPQYWELFKDRETPD
                     KGRFYNAIPGWVVHDINLKKLEAYNLTETFINFDPGSQTALMTAIVSAYEKGEPVLAY
                     YWEPEWIMGMLDMTLLEEPEYDEKLWNEENKFACAFPAARVHVGINAEFAKKNPEILA
                     FLEKYETTLAQNNQALAYMQQEEATVEEAAIWFLKNYKEVWKAWLPGDVAGKVEAALA
                     GK"
     gene            6649..7533
                     /locus_tag="PK146_01390"
     CDS             6649..7533
                     /locus_tag="PK146_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163687.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HPE90921.1"
                     /translation="MAENVIDVKELWKVYSKKDIDLNVEDEEMVESLDKSSDSVVAVR
                     DVTFSVKPGEVFIVMGLSGSGKSTLIRCLLRLVDISWGSVTINGQDVTAMNKKELTEF
                     RRNQAAMVFQHYGLLPHRTVIENVAFGLKLRGIPKKERRERARETLNIVGLEQWGDYY
                     PASLSGGMRQRVGIARALVADTPLLLMDEPFSGLDPLIRREMQDELVRIQEELHKTIF
                     FVTHDLDEAMRLGDRMAVMRNGEIVQIGRPPEVFANPADDYVARFVQDKRDEIRKTDE
                     QMAALAAQSEKAGTPDVS"
     gene            7523..8374
                     /locus_tag="PK146_01395"
     CDS             7523..8374
                     /locus_tag="PK146_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HPE90922.1"
                     /translation="MFPEAFKYSVAKSVNSAIKWLLNTHGSAFDSFSSGILSLLLWVQ
                     GILVSIPWWAYIAFIALMAWIATRKIIPVIVLAVLPIFIGMFGLWHLALETLALVITS
                     VVIALAIGIPMGVLMAEARYFAEFIKPVLDAMQTMPSFVYLIPAMMLFGLGKVPAVIA
                     TLIYALPPVMRLTCLGLQQVPRPVQEAALAYGATRWQLLKEVRIPLAMPSILAGVNQT
                     TMMALAMVVIASMIGARGLGQEVLMAINRIDVGRGFEAGISIVIMGIVIDRITQGLAK
                     RWDPNRK"
     gene            8719..9705
                     /locus_tag="PK146_01400"
     CDS             8719..9705
                     /locus_tag="PK146_01400"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HPE90923.1"
                     /translation="MILRKASLLALALLLCLVPAALATETAPSKFTLFVDAGWVKANA
                     SSVVLVDARASTLYAKGHIPGAANAEWTYFANVKGQPGNPGWGEIFPKDTLAKKIGAL
                     GINGKKPIVVYGDCGGWGQEGWVAWILRLSGIENVRVLDGGYNAWLAAGGKASTTATK
                     PRAVAFSIASFQGGYKVTTPWLASKLGEVKVVDARTPFEYEGGRIFQERRGGHIPGAI
                     NIPYDVVFKPDMTVREVEELDAIFKSYGLDPEDEIVVYDTAGVRAAHLVMVLRMAGYD
                     KARNYDSSFHEWAGTPELEVVQGKEPGGQAPAIEEIAEEEPPQPEAAEGQSS"
     gene            9709..11040
                     /locus_tag="PK146_01405"
     CDS             9709..11040
                     /locus_tag="PK146_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPE90924.1"
                     /translation="MSITVEIAGGGSYSLDPPVDGQDLLQKLGAGCDRKIVAWHVNRY
                     LRPLDWPLREDSLIDFVDTSSFEGMSVYRGTLVFALSLACRGALGDGISVRHSISDGY
                     YCELQSGPASPEQTGLVRDAMEYIIRRDLPVRREVLSIDSAERILLQQNEPDTADLLK
                     YAGVDPVNLYQCDGVYGFFYGPLAPSTGYLSSWELVPFREGMVLRFPTVAYPLELPPF
                     TPAEKLADVFNEYGDWLQILGVKTMENLHSQVIDNKARELVLVSEALHSQKLNSMSER
                     ITAGHKKRLICISGPSGSGKTTTAERLSIHLRAMGVKPLAVSLDDYFLDREKTPRDSE
                     GNYDFEAIEALDLELINGQLEDLLEGREVTLPRFNFLTGKREEGPRVRMGKDDILIIE
                     GIHGLNSRITSQIPADIKFSVYISPLTGISLDRHNRTSTTDNRLMPVRGEM"
BASE COUNT         2207 a         3236 c         3099 g         2507 t
ORIGIN      
        1 tactcgtgaa tcgtgaaccg ggaggggcaa aggcagggcc ccgtccaccg ttagcgccct
       61 tgatccagcc ccctgcctct cagattttga ctctcccaca aaaagacccg ggttttcctc
      121 tctcttgcac ccctctgcgg ccctctgtga cctctgtggt gagatggttt gattttggct
      181 ttggatcgtt ccctcccgtt caccgttaac cgtttacgca cttgatcctg gaagtcacga
      241 gtcatgcgtt acggtcttgc agccttgctt tccgttatcc gagttacgag ttacgcgtta
      301 cgagtcacgc gttacggtct tttaaagccc cagactcttc ttcacgtggg ggatcagccc
      361 cccgtcctcg atcagaccct tcacgaaatc cgggtagggc gggaacttga aggtcccttt
      421 gggtgtcacg atctcgctcc tgtcaaaatc gatggtgact tcctgaccct tctcaagcgc
      481 gtccaccgct tccgggctta ccacgatggg cagcccttcg ttgagggcgt tgcggtagta
      541 gatcctggcg aaaccctttg ctatgatcgc gccgatgccc cttaccttga ggcatgtgac
      601 ggcctgctca cgggaagagc cgcagcccca gttctttcca ccaacgatga tgtcccccgc
      661 ctgtgcgttc ctggtgaact cggggtcgag atcctcgagg gcgtacttgc ccatctcggc
      721 gcggtcagag acggtgtagg tgtacttgcc cgggaaaatg acgtcggtgt tgacgtcgtc
      781 cccgtatttc cagacctttc cggtgatctt catggtccta aagcacctcc ctgggatcgg
      841 agatctcgcc cttgagagcg gaggcagcga ctgtcatcgg gctggcaagg tagatgttgc
      901 tctccttgtt gcccatacgt cccttgaagt tcctgttggc tgtcgaaatg catgtctcgc
      961 ccggcgccag gatcccttcg tggttgccca tgcaggggcc gcagcccggg ttggtgatga
     1021 cgcacccggc gtccaggaag acatcgataa gcccttcctt catggcctgg cggaagaccg
     1081 accatgaagc gggaataacg atcgtcctta ccgcaacttc cttgtccttc aggatatccg
     1141 cggcaagccg caggtcctcg atgcgggcgt tggtgcagct tcccaggaac gcctggtgaa
     1201 tgggtgtgcc tttcacttcc ccgatggggg catagttgtc gacggtatgg ggtttggcaa
     1261 cacatggctc gatgtcgccc aggtcgtagt tgagctcctg tgcgtatacg gcgtcttcgt
     1321 cggcccacat ggcctcccat tcgtccgtct tggcgatggg tttgatgaaa tccaggacct
     1381 tctggtcagg ggggcatacg gcgttcttcg cgccgatctc gatccccatg ttgcagaggg
     1441 tcatcctctc cgccagggac atctcctcga tggccgggcc gtggaactcg atggacatgt
     1501 agtcagcgcc gtcggccttt atatcgccag cgatcttgag tatcaggtcc ttggcggata
     1561 ctcctttgtt gaacttgccc ttgacggtga tcttcatcga ctccgggacg cggaaccata
     1621 tctgacccgt agcccaggtg gctgccatct cgcttcggcc tatacccgtg gagaaagctc
     1681 cgaaggcgcc gtaggtgcag gtgtggctgt cgcttcccac catgaccaga ccgggcagtg
     1741 caaagccctc ttcgcacatg atctggtggc agacgccgcc cttgcttgtg acatcgtaga
     1801 agtacctgat gccctgttcc ttgaccactt cccggatctg cttgtggttc tccgcgtgct
     1861 tgtctgttgg tgccggaatg gcgtggtcaa ggatgaacac caggtgatca ggtttccata
     1921 ccttgtcaac gccgatcttc ttgaaggttc cccagatcgg tgccgcgttg tcgtggctca
     1981 tgcagaagtg aggttcgact atcacgacct gcccggccga aacggattcc ccagctgcct
     2041 tgccaagaac cttttcagcg aatgtctttc ccatgccgac ctctccctcc tacttgctgg
     2101 cggccttttc cttgaggtac tcgatgaggc cgccggcctt catgatgtcc atctcaaggt
     2161 ccgccacagc gtcgccgtga aaggagttgc cgttggtgag atccctgatc tcgcccttct
     2221 ccaggtcgac ctcgagatcg tcgccgtcct tgatcttccc atccttgatc gcctggctga
     2281 gccccttgac gaagaggacc gggtatccgt tgttgatggc gttgcggtag tagatccgcg
     2341 agaaggattc ggccaggatc accggcactc cggcgtattt aaggcagtag acggcgtgct
     2401 cgcgggatga accgcatccg aagtttgtcc cggcgacgac aaaatcgcct tcctttacct
     2461 cttttgcgaa attttccttg ccgggcaggt actcgaagga gtactgcggc attttcctgg
     2521 gatctgtctc ggccaggtac ttgttgtggt agatgaggtc ggtgtcgatg tcatcaccaa
     2581 agacccaggc ctttccgcga aggactttat tttccatcag tcgtcccctc cctatccgag
     2641 gatatccctg ggatcagtta tctttccgcg aatggccgtg gccattgcgg tcatcgggct
     2701 tgccaggtaa gtatggcttc ccttgccaac cttgccgata aagttcctgt tggtggtcga
     2761 catgcccacg tcgccgctcg ggagagcccc gtagtgctcg gcggtgcata gcgcgcaggt
     2821 cgggttggta aagatgaccc cggcctcgag gaacttctgg atgtagccgg cctcggcgca
     2881 ctgaagcatt acttcccggg tagccggggt aatgatggcg cgcattccgg gagccacttt
     2941 ggattttgaa gccttgagga cttcgaatgc tgccgagatg tcctcgaaac ggccgttgga
     3001 gcaggagccg atgtgcacct ggtcaacgtg ctcgccggcg acctcgcgga ccttcttgac
     3061 gttgtccggc gcgtctgggc aggatgtcat gggttcaagg ttgtccacgt tgaactccag
     3121 ctcctggcag tagacggcgt ccgggtctgc tgaaaggctc ttgaccctgt ccacgacctc
     3181 cttgccggcc ctctcaacca gccagtcgag gaccttgccg ttgggcatta tgagccctat
     3241 cttgccactc atttcggtga ccatggagca gagggttatc ctgtccgaaa gggacgacct
     3301 ctcgattgtg tcgccgtaga actcggtgga caggaagaac attccgtcgg cgccgagagt
     3361 ccccacgatg tgcgtcagca ggtcgcgcat gcagacgccc tttgcgaact tgccgttgac
     3421 ggtgatcttc atcgtctcgg gcacgcggaa ccagttggtc ccctcgaacc acgatgccac
     3481 gatgtcggta ttcccaacgc cggtggcgaa ggcgcccacc gccccgagga ggttcatgtg
     3541 gctgtctgtc ccgaggatga cgtcgccggg ttttacaagg ccgttctcaa gaagaacatg
     3601 ctgtccgata ccccagtcca cgtcgaagac cttcttgatg ccctgcttct ttgcgaactc
     3661 ccggatgatc ttctggtttg tcgagacctt ctccgaatgg gatggagcct gaaggtcgaa
     3721 ggtgaaggcc accttgtcgg gatcccatac cttgcccttc ttgtcggtga cttcttcgaa
     3781 ctgcagaacg cagttggcgc cgccgaagtc gcgggccgtc gagaggtcga tattgcacca
     3841 gaccctctct ccgaccttga cctccttgcc ggctgcccgc cggatgatct tctcgatcat
     3901 cgttttgccc atgggtcacg cacctcctgt tttggatcgt tcctgctaga gaagcccgag
     3961 gaacgacttg atggtctcga agttgatgaa atcacacttg ttgatgacca ctgcctccgg
     4021 gcagcgatac ccgccgtcgc cctccttggc gtgaacggct atgcagtgac agatcctcga
     4081 gggtatgccg ttacgcatgg ccaggccgac accggagaag gggtggcgca gatccttgcc
     4141 catctgggac ttcactgcct tgccgtcctt cttttcgtac tccaccagtt tgccaacgtc
     4201 atggaggatc gcgccggcga taagtgtgtc atggtcaagc tcgaacccgc cgttgtcctt
     4261 gtagaggggg ttgtactcct cgtagataac ctttgccatt ctcgtcacgc ccctgttgtg
     4321 gacgttgaga gaggctgggc agtcggggat gagcagtgtg aaggggatct tgtccatatc
     4381 ttccggctcc catccgccgg tcttgagcgc atcctcgatg gttgctacga ccttttccct
     4441 gagctccttg tcctggatcc agtcgatctc ggggatcatc ttcacgatcc tgtctctcat
     4501 gttccatccc tccaatggtt tttgagactc aagcgcctct atctatttac ttgacctcaa
     4561 gggccagccc ttcctgtaaa gtcctgtaga tcctctctat gtgccccgcc gaaagcgatt
     4621 gggcccttgc cacgtcatga tcgatgatag cctcgtaaat ggtctggtgt tcattgaaga
     4681 aggcctcctt gtctgcaagc agggagtaga tcctttcgtg ggtggccatg atcaggttga
     4741 aggtcatctt cactatattg atgaacacga aattgtgggt agcctctgcc agcatcaggt
     4801 ggaaatcgaa atcgtcctgg gatctctttt cgatgtgctc caggtttttc tcggtcctgg
     4861 agagaacctt gcgcagcttc atcaggtccg aaggcatggc atgcatcgcc gccgctccgg
     4921 caattgccgg gtccagggct ctccgggcct ccataagctc cagcacggcc tcgtcctcca
     4981 gcggcatgtt cctggaatcg gccgtctctc cccggggttt tcggacaaaa cgccccttgc
     5041 cagggacgct gtcgataagc cccagcagct ccatgaccct gaaggcctct ctcagggagc
     5101 ttctgctcac acccatctcc gacatgagct gccgctccgg ggggagcggg tctcccggca
     5161 gcagttcgcc cgatgcgatc agctccttta gctcgttgac cacctgttcg tatatccttg
     5221 tgtggcgaag tcttttttcg atcatatctt ccctcctcgg tggaccaccc agtacacttt
     5281 atataccagg tagacccttt gtgcaagtgt tcgcaataag actaaatgtt caggcaattt
     5341 gaaactttat tttgcataca aacgtttgac aacttccccg gattcaacta acataacctg
     5401 gtccgggggg tataggtacc ggctattcaa tgtgaaatgt tgtgtcggac aacatttcac
     5461 aactcttccc aagcatcatt ctgtcgtttt tcaagtggag ttcctgtctt ctgttctccg
     5521 cgagtcccgc ggaaacggta cctttgacga ccctcgtggg gtcggaacaa ttcgaggatt
     5581 tccccccatc ttctgtttct gcatgtgtaa atccaaagga gggattttgt catgaaagca
     5641 ggacgaaaaa cttttctgat ccttgccctt gcccttgtgg tggggctgac ctcgatcccc
     5701 gccgcatcag cagccgggaa tgtagtcatg gtcgatttca gctggagcag tgtccagatg
     5761 cataaccggg ttgccggttt catcctggaa catggttacg gcttctcgcc ggattacatg
     5821 ttcgctgaat ccgttccagg gctcacaggc cttcagcagg gcgacatcga tgtagccatg
     5881 gagatgtgga tagacaacgt tctcgagtgg tacaccgatg ccgttgagga aaacaaacag
     5941 gttcttgacc ttgggtcggt tttccctgat gcccctcagg gatggtatgt acccacctac
     6001 atgatcaagg gtgacaagga acgcggtatc gagcccatgg ccccgaacct gaagtcggtc
     6061 tttgaccttc cccagtactg ggagctcttc aaggatcgcg agaccccaga caaggggcgg
     6121 ttctacaacg ctatccccgg atgggtcgta cacgacatca acctgaagaa actggaggct
     6181 tacaacctca ccgaaacatt catcaacttc gatccgggtt cccagacagc gcttatgact
     6241 gccatagtct ctgcctatga gaagggcgaa cctgtccttg cctactactg ggagccggag
     6301 tggatcatgg gcatgctgga catgaccctg ctggaggaac ccgaatacga cgaaaagctc
     6361 tggaacgagg agaacaagtt cgcatgcgct ttccccgcag ccagggtcca tgttgggatc
     6421 aacgccgaat ttgccaaaaa gaacccagag atccttgctt tcctcgaaaa atacgagacc
     6481 acccttgccc agaacaacca agcccttgcc tacatgcagc aggaggaagc cactgtcgag
     6541 gaagcggcta tctggttcct caaaaactac aaggaagtct ggaaggcatg gctccccggt
     6601 gatgtggccg ggaaagtcga ggcagccctt gcaggcaagt agacgcccat ggcggagaac
     6661 gttattgatg taaaggaact ctggaaggtc tattcgaaaa aggatatcga tctcaacgtc
     6721 gaagacgaag agatggtcga gtcgctggac aagtccagcg actcggtcgt cgccgtaagg
     6781 gatgtaacct tctccgttaa acccggcgag gtctttattg tcatgggtct ctcgggcagc
     6841 ggaaaatcaa cgctgatacg ctgtcttctt aggcttgtcg acatttcctg gggcagcgtg
     6901 acgatcaacg gccaggacgt aacggccatg aacaagaagg aactgacgga attccgacgc
     6961 aaccaggcag ccatggtctt tcagcactac ggactcctcc ctcaccggac tgtcatcgag
     7021 aacgttgcct ttggcctgaa gcttcggggg attccaaaga aggagcgcag ggagagggct
     7081 cgcgagacgc tcaatatcgt ggggctggag cagtggggtg attactatcc tgcttccttg
     7141 agcggaggga tgcgccagag ggttgggata gcccgggccc tggtggcgga cacacccctt
     7201 ttgcttatgg atgagccctt cagcggtctc gatcccctga tccggcgcga gatgcaggat
     7261 gaactggtcc ggatccagga ggaactacac aagacgatct tcttcgttac ccatgacctg
     7321 gacgaggcca tgcgccttgg tgaccgcatg gcggtaatgc gaaacggcga gatagtccag
     7381 ataggcagac ctcctgaggt ctttgccaat ccggccgacg attatgtcgc gaggttcgtg
     7441 caggacaagc gtgacgagat ccggaagacc gacgaacaga tggccgcgct ggctgcgcag
     7501 tctgaaaagg cggggacccc tgatgtttcc tgaagccttc aaatactcag ttgcgaagag
     7561 cgttaatagc gccattaaat ggctgctgaa cacccacgga tcggccttcg acagtttctc
     7621 atccgggata ctgagtcttc tgctgtgggt gcagggcatt cttgtctcaa tcccctggtg
     7681 ggcctacata gccttcatcg ccctcatggc ctggatagcc acaaggaaga tcattcctgt
     7741 tattgttctc gcggtgctcc cgatctttat cggcatgttc ggactctggc acctggcgct
     7801 cgagacgctg gccctggtaa taacatcggt ggtgatagcc cttgccatcg ggatacccat
     7861 gggcgttctc atggctgagg cgagatactt cgcagaattc atcaagcccg tgctggacgc
     7921 catgcagacc atgcccagct tcgtatacct gatcccggcg atgatgctct tcggcctggg
     7981 gaaggtcccg gccgttatag ccacactgat ctatgccctt cctcctgtga tgaggctcac
     8041 atgtctgggg cttcagcagg tacccaggcc ggtgcaggag gcggcgctgg cctacggcgc
     8101 gacccggtgg cagcttctca aggaggtacg catacccctt gccatgcctt cgatcctggc
     8161 cggtgtcaac cagaccacca tgatggctct ggccatggtc gtaatagcgt ccatgatcgg
     8221 agcccgcggc ctgggccagg aagttctcat ggccatcaac cgtatagacg tcggaagagg
     8281 attcgaggcc ggcatctcca tcgttatcat gggtattgtc atcgaccgca tcacccaggg
     8341 cctcgccaag aggtgggacc cgaacaggaa gtaaacctgt aactcgtgac tcggaagatc
     8401 aaaaggcgtg aacggtaaac agtcaacagt aaacggtgtc acaaaaagct ttgaaaagtt
     8461 gtccttaccg ttcaccgttt accgtttacg cacttgactc ttcgtttaac gtctcacggg
     8521 tttttctcct tcctgctgcc tgcagcctga ctcctgtctc cccgggctta cgctcttccg
     8581 tctcacgtct cacgtcttac atctcacgca tttggttttt acgtactttc cccatcgacc
     8641 gggaaggcct gcaacggtta gaatatcatc tgaggacctg taaccggtcc caccttccaa
     8701 catccaaagg aaaggatgat gatcttgaga aaagcatctc tgcttgccct tgctctcctt
     8761 ctgtgcctcg ttcccgccgc gctggccacg gagacggctc cctcgaaatt taccctcttc
     8821 gtcgacgccg gatgggtaaa agctaacgct tccagtgttg tcctggtcga cgccagggct
     8881 tccacgctct acgccaaggg acatatcccc ggggcggcga acgctgaatg gacctacttc
     8941 gccaacgtga aggggcagcc gggcaatccc ggctggggag agatatttcc caaggatacc
     9001 cttgcgaaaa agatcggcgc tctcgggata aacggcaaga agcccatcgt ggtctacggc
     9061 gactgcggcg gctgggggca ggaaggctgg gtggcctgga tacttcgcct ctcggggata
     9121 gagaacgtcc gtgttcttga cggagggtac aatgcctggc tcgccgcggg cggtaaagcc
     9181 tcaacgacgg cgaccaagcc cagggccgtg gccttctcca tagcctcctt ccagggaggc
     9241 tacaaggtca ccactccctg gctggcatcc aagctcggcg aggtcaaggt tgtagacgca
     9301 aggaccccct tcgagtatga aggaggaagg atcttccagg agcgccgggg agggcatatc
     9361 cctggagcga tcaacatccc ctatgacgtc gtcttcaaac ccgacatgac cgtccgggaa
     9421 gtggaggaac tcgacgccat attcaaaagc tacggtctcg accccgagga cgagatagtg
     9481 gtctacgaca ccgccggcgt aagggcggcc cacctggtta tggttctgag gatggccggc
     9541 tacgacaagg cccggaacta cgactcctcc ttccatgagt gggccggaac tccagagctg
     9601 gaagtggtcc agggcaagga acccggcgga caggcacctg cgattgaaga gatagccgag
     9661 gaggagccgc cccagccgga agccgcggaa gggcagtcct cctagaatat gtccattacg
     9721 gtcgagatag ccggcggggg ctcctacagc ctggaccccc ccgtcgacgg ccaggacctt
     9781 ctgcagaagc tgggtgccgg atgcgacagg aagatcgtcg catggcacgt gaaccgctac
     9841 ctgagacctc tggattggcc tctcagggaa gactcactta tcgatttcgt cgacacctcc
     9901 tccttcgaag gaatgtcagt ctacaggggc acgctggtct ttgccctatc cctggcctgc
     9961 cgtggagccc tcggcgacgg aatatcggta cgccactcca tcagtgacgg atactactgc
    10021 gaactccagt caggaccggc ctcgccggaa cagacgggcc tggtccggga tgcgatggag
    10081 tatatcatcc gcagggacct tcccgtaagg agggaggtcc tcagcatcga ctccgccgag
    10141 aggatacttc tgcagcagaa cgagcccgac acggcggatc ttctcaagta cgcaggagtg
    10201 gatccggtga acctgtacca gtgcgacgga gtctacggct tcttctacgg ccccctggca
    10261 ccttcgaccg gctacctctc ctcctgggag ctggtccctt tccgggaagg gatggtcctg
    10321 aggttcccta ccgttgcata tcccctggaa cttcccccct ttacccccgc cgagaagctg
    10381 gccgacgtat ttaacgaata cggcgactgg ctacagatcc tcggggtaaa gaccatggag
    10441 aaccttcaca gccaggtgat cgacaacaag gccagagagc tcgtcctggt tagcgaggcc
    10501 cttcacagcc agaagctcaa cagtatgtcc gaacgcataa cggcagggca caagaagaga
    10561 ctgatctgca tctccggtcc ctcggggtcc ggcaagacca caactgcgga gaggctctct
    10621 atccatctcc gggccatggg agttaagccc ctggcggtat cactggacga ctacttcctg
    10681 gaccgtgaga agaccccccg cgactctgaa ggcaactacg acttcgaagc catcgaagcc
    10741 ctggacctgg agcttattaa cgggcagctt gaggatcttc tggagggcag ggaggttacc
    10801 ctcccgcgat tcaacttcct taccggcaaa cgtgaagagg gtcccagggt aaggatgggc
    10861 aaagacgaca tccttatcat cgaggggatc cacgggctca acagcaggat caccagccag
    10921 ataccggcgg acatcaagtt cagcgtctac atctcgcctc tcaccggcat tagcctggac
    10981 aggcacaaca ggaccagcac caccgacaac aggctaatgc cggtgagagg cgagatgtag
    11041 acgctgaac
//
LOCUS       DAOPKQ010000019        10678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_6484_length_10678_cov_4.654900, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000019 DAOPKQ010000000
VERSION     DAOPKQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6484_length_10678_cov_4.654900"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PK146_01410"
     CDS             complement(<1..462)
                     /locus_tag="PK146_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90925.1"
                     /translation="MIIAWVLAVLLFSLSWLTDTERSIEAARTAWSLFRRILPMFVIV
                     LVLTSLSLGLISEEVLLELLVGKGLLAGSAIGAAAGSVAAIPGFVAFPLSGILLGKGV
                     PYTVIAAFTTTLMMVGVVTFPLERQYLGVRAALVRNLAGLAMALIVSVAIGV"
     gene            649..1863
                     /locus_tag="PK146_01415"
     CDS             649..1863
                     /locus_tag="PK146_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90926.1"
                     /translation="MQEAILAALSLSSMLVSRGHEAAVVGGAVRDLLLSRRATEADLA
                     TSAPMEEILDIWPGSPVLGRPPAVTVIVLSRGCRIDVSTFQGKDLEEDLGRRDLTINA
                     MAMVPDGTIIDPWGGRSDLAGRLLRFTGDPVERLIADPLRAVRIARFVSELPGFRVDQ
                     GSLKTCREFASRVAVMPLPRIGREVLHALEGDLPAFLETLEQGGILDPILPCLKGLSP
                     FQRDQVFSRIRNACALTADPAVRAATLLADEGTTVTGTLSAWNWPNSLSREISALVEN
                     LTVALDGCDVRTWARLFRSRGADWIGKLSLLGFIFSLNSEPDTGVWVQRRVGSIPFIV
                     RLQSFGQRITGEEVMALTGLESGPEIGRIMDDLDEAVALGEVRNREDALKWVSSAGGG
                     HLRNSFSFPNPG"
     gene            1913..3025
                     /locus_tag="PK146_01420"
     CDS             1913..3025
                     /locus_tag="PK146_01420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE90927.1"
                     /translation="MDRSEMGKTAAEFQKQQRKRKIATCFVLSGWFATMYITDTLFGY
                     HPIVSIDGALALFFFICSFAVASVSSRRKRSFLGITAIAGLLFISSFPLSFTLLGIPV
                     TILLLRQVMTLLFCALVGPYFIKPKGFAFFLVLLYNALCFYYPILRVRELDETMAYVI
                     VTYSFTLLFSYFFLDILRMNLGHVSSLISAKKDAENMALRDDLTGVYNRRYCDNLLRV
                     LTENETEKALLFIDLDDYQNVCQAHGHLVGDAYLKRSAEAVLESVRSGDIVSRFGGNQ
                     FVVVLDSGDLSLAETVARRIRKAVGEVIPEFRSVSVGVTKIIPGITQQAVLVNADTAL
                     YRSKHSPLGNISIVDPDNPRIEPEHWPETDMRLQER"
     gene            3210..3725
                     /locus_tag="PK146_01425"
     CDS             3210..3725
                     /locus_tag="PK146_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE90928.1"
                     /translation="MQNNTLEVILSRRSIRRYTDRPVEDSSIETLLKAGMSAPSAHNQ
                     QPWEFIVVDDRKTLEEIPLFHPYSRMLLKAPAAIVVCGITRDIPAPQFWPQDCAAATE
                     NILLAAHALGIGSVWLGVFPHEELVSRLSELLAVPTDITPFSIISLGYPDEGKGPSER
                     YDAARVHRNRW"
     gene            3819..4970
                     /locus_tag="PK146_01430"
     CDS             3819..4970
                     /locus_tag="PK146_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE90929.1"
                     /translation="MTLISVLGVASVAPAFPEIIRALGVHQEDAAWLISAFTIPGLLF
                     TPVTGFLSDRYGRRKILIPCLFLFGIAGGACFLARDFRTLIFLRFLQGVGSAPLNALN
                     VALIGDFFKGNDRSAAMGYNTSVIGVGTAIYPALGGLLAMAGWNYPFLLALVALPVGF
                     WAYLGLNEGDRFPPGGQSAGGAPRPLLRELARKGVPVLLFASMMTFVIQYGGFLTFLP
                     FYLESGYGATPLTIGLVSAAMSISSAVTSYRAGWFSARFSSRSLMAIAFILYCSALLF
                     ILHVSGCSGMIVPAMLFGIGQGINIPNLLTTLTNNTPEENRAMVMSLNSMSLRLGQTL
                     GPPLFGMVFASRGIEAVFTTGAVMAGGMAVVIFGTMRKIGGTSEVGHDA"
     gene            5130..5480
                     /locus_tag="PK146_01435"
     CDS             5130..5480
                     /locus_tag="PK146_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE90930.1"
                     /translation="MFCSCKDMRAIRRENLQGGPGGGMFYPLIAPGEKPEGSHLKMVA
                     RVELDPGAAVGEHTHTGDEEVYHIISGRGIFIEDGVQVKVGPGDTAVTLDGHSHSIIN
                     TGDEPLVFLAVIAE"
     gene            5730..7400
                     /locus_tag="PK146_01440"
     CDS             5730..7400
                     /locus_tag="PK146_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE90931.1"
                     /translation="MILKRAVLLFLVLVSVILNATVSTAFDVSAVRGPHIRDLYMVIY
                     RDPDSQILALQKGELDVLGDVTRPSDIERLSSNQDVNLSISRGFHLFFMGFNLRKGPW
                     NDKTLRQAASMAIPSIQIVRDIFSGYSEPVNTYLPPVSPYHETDVTTWEHDPRGARVL
                     LEEEGWRWSPDGRLIPPGEESPLKGVKILSPTAQVAPTTAEISSRIADALKEIGIPVS
                     VEPLDFSTMIRRLDLHDFDLFVMAWSLTRDPDSLFAFFHSSMDVTGGYNIQGLHDPLL
                     DEVLEELRWAPDRQTAEGAARKAQQMLSEISPWIPVYSRYMVAAVRKGWDGVISTQAT
                     SADNIWTLLNMRSSDGSERPVFWCLSDEPRSLNPLSTGSAYDWQVLGLIYDGMIAVDP
                     ETLDDIPWLATKWEVETVSGEDGDHTRLTFHLRDRVKWHDGSPFTSKDVGKTLEFLRD
                     NEIPRYFDNVRDILKVETPDDLTVIVTMKNVSFWHLHNIGGSPVFPAHLLEGVDEWET
                     WQPAAEPHPSVKGLTSLVGTGPFVFREYRQGEYVHFVSNEDFWLIGGD"
     gene            7404..8381
                     /locus_tag="PK146_01445"
     CDS             7404..8381
                     /locus_tag="PK146_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE90932.1"
                     /translation="MWSYWSRRALGAVAVLLLVIAANFFLFRVMPGDAVSTIVDPRFS
                     PEAKERLRELFGLDLPLYRQFLIYVRRMLTFDLGISFLSQRPVWEELKSRIPNTVALL
                     GSALFLSSALGIWLGVKAARRRGGLTEKLVLVSGSVSFSFPSFFVQLVLLLFFAYTFP
                     VFPLRGSVSIPLPEGAFARALDYLWHLLLPVGSLVLLGFGSWAIYVRNLMVKTLGEDF
                     ILLARARGLPEGRILWNHAFRSALPPVLTIFLLALPGIVSGAVITETVFSLHGVGKYL
                     LDSIMGHDYPAAGAAFYILGLLTIVSNLAADAAYALADPRIRVGRRAQR"
     gene            8381..9571
                     /locus_tag="PK146_01450"
     CDS             8381..9571
                     /locus_tag="PK146_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE90933.1"
                     /translation="MKVPRWSFITFFLILAIAVIGPMSLSKGPQETVAKPFAPPVWSG
                     GDAPPPFSGNLSPNSKEMNIEWDWDAPENLTLRGQLTFDHDVVARLVWTTPEGSKYTL
                     SSFHGSGVWSIDLDARDMTFKTVLGIPPFASATSLLFPSRGLHALSLEGPSPSEGSME
                     LVLPGGRWGLLGTDQRGRDVLSLFIVGIRVSLLVGISATFIASLLGLSIGLASGYLGG
                     VADSLIMRIVDVLLSIPILPILMVLVGIWGKGLWQLVLILGLFSWMGTARTVRSLALS
                     LRESCYVENLRSIGAGSWYILYRHLLPEAMPIVLAAVSLGVPGAILAEAGLSFLGLSD
                     PRIISWGRMLHEAHGFGAFTAGAWWLIIPPGLGISIICLVFLDMGRSIEEMADPRLRG
                     GGQG"
     gene            9586..10350
                     /locus_tag="PK146_01455"
     CDS             9586..10350
                     /locus_tag="PK146_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE90934.1"
                     /translation="MSITYRTPARNVPAVRDVSLEIPAGRITAIVGGSGGGKSSLIYG
                     ILRLLPPRTELSGSIFFDGTDLVSTDEEWMREFRWKRAALITQGAMNSLTPVLTVGYQ
                     ISEVLQVRLGLSRREAEKRTLELLAMTNLTQIHARKFPHELSGGEKQRAVISMAVSCF
                     PGLLIADEPTSALDMITQAEVVTTLVGMVRDQGMGLLLVTHDLPLAISVCDDLAVMYE
                     GRIVETGTPSKILRSPSHEHTRKLLSAFSFKGGARP"
     gene            10347..>10678
                     /locus_tag="PK146_01460"
     CDS             10347..>10678
                     /locus_tag="PK146_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE90935.1"
                     /translation="MRELLLSAKGVSVSFLSPRGTTEALKGVDMDVPTGGCVAVVGES
                     GSGKTTLLRACLGLVPMFEGSVTLFDRDLSFCGSKELVTLRRRCGYVPQDPFGCVPPT
                     LNTLDAAA"
BASE COUNT         2204 a         3088 c         3052 g         2334 t
ORIGIN      
        1 gaccccgatg gcgacggaga cgataagggc cattgccagt ccggccaggt tcctcaccag
       61 agccgccctg acaccgagat actgtctttc cagggggaaa gtgaccactc cgaccatcat
      121 gagagtggta gtgaaagccg cgatcaccgt gtagggaacg ccttttccaa ggagaattcc
      181 cgacaacggg aaggccacga aacccggaat ggctgctacg gaaccggcag cagccccgat
      241 ggcgctcccc gcaaggagcc cttttccgac caggagttca aggaggacct cctcgctgat
      301 cagacccagg ctcagcgaag tgagcaccag gacaataacg aacatgggaa ggatcctccg
      361 gaagagtgac caggcagtcc tggcggcttc aatggatctc tctgtgtcgg taagccagga
      421 aagggagaag agcagaaccg caagcaccca cgcaatgatc attggtctcc atgaccccct
      481 ttgacgtcgg aaatattatc gcctttctat tctacagcct tccgggcctt gctttttcca
      541 tggaccggga acctcagaga aagattcttc agaccggata tgtatcctct ctgaaaagac
      601 ctcgccgctg cggtaaagtg atacaattca tgagaggtga tgagaccctt gcaggaagct
      661 atcctggcgg ccctctccct ttcgtcaatg cttgtctcac ggggtcacga ggcggccgtt
      721 gtcggaggcg cggtccggga ccttctcctt tcccggaggg cgacggaggc ggaccttgcc
      781 acgtcggccc ctatggaaga gatcctggac atctggcccg gatcgcccgt cctgggacga
      841 ccgcccgccg tgacggtgat cgttctgtcc agggggtgcc ggatagatgt ctccaccttc
      901 cagggcaagg acctggaaga ggacctgggg cggagagatc tcacgataaa tgccatggcg
      961 atggtccccg acgggaccat catcgatccc tgggggggga ggagcgatct tgccgggagg
     1021 ctgctcaggt tcacaggcga ccctgtagag agactgatcg ccgatcctct gagagcggta
     1081 aggatcgcca ggtttgtctc ggaactcccc gggttcaggg tcgatcaggg gtccctgaaa
     1141 acctgccgcg agttcgcaag ccgggtggct gtcatgcccc ttccccggat cggcagagag
     1201 gttctccacg cccttgaagg tgacctgccc gcctttctgg agactctgga acagggaggt
     1261 atccttgacc ctatcctgcc ctgcctgaag ggtctttcgc cgttccagag ggatcaggtt
     1321 ttctcaagga tccgcaatgc ctgcgccctg acggccgacc cggctgtccg ggcggccacc
     1381 ctgcttgccg atgaaggaac aacagtaaca ggaactctat cggcatggaa ctggccgaac
     1441 agtctgtcac gggagatatc agcgctggta gagaatctca cggtggccct tgatggctgt
     1501 gatgtccgca cctgggcacg gctgttcaga tcaagggggg cagattggat cgggaagctg
     1561 tcacttctgg ggttcatctt cagcctgaac agtgaacctg acaccggggt ctgggtccaa
     1621 aggagggtgg ggtcgatacc tttcattgta cgcctgcagt cattcggcca gaggatcacg
     1681 ggagaggagg taatggcgtt aaccggtctt gagagcggac cggagatagg caggatcatg
     1741 gacgacctgg acgaggcggt agctctcgga gaggtcagaa accgggaaga tgccctgaaa
     1801 tgggtctctt cggcaggagg aggacacttg cggaactcct tctcctttcc taatccgggt
     1861 taaaatagta tccttattgt cagggtcttt tttcgaaaac ggggggttac cgatggatcg
     1921 gtccgagatg gggaagactg ctgcagaatt ccagaaacag caaaggaaaa ggaagatagc
     1981 gacttgtttc gttctatcag gctggttcgc taccatgtat attaccgaca ccctctttgg
     2041 ctatcacccc atagtgagta tcgacggtgc cctggcgctg ttctttttca tatgttcatt
     2101 tgccgttgcc tccgtatcct cgaggaggaa gcggtctttt ctgggcataa ccgccattgc
     2161 cggcctgctc ttcatatcct ccttcccgct ctccttcacc ttgttgggga tcccggtgac
     2221 gatccttctt ctaaggcagg tcatgaccct tctcttttgc gcgctggtgg gtccctactt
     2281 catcaaaccc aagggattcg ccttcttcct tgtgcttctg tacaacgccc tctgtttcta
     2341 ctacccgatt cttcgggtaa gggaactgga cgagaccatg gcctacgtta tagtgaccta
     2401 ctcctttacc ctcctgttct cctacttctt cctggacatc ctgaggatga acctgggcca
     2461 tgtgtcctct cttatctccg ccaagaagga cgccgagaac atggctctca gggatgatct
     2521 gacgggcgtt tacaacagga ggtactgtga caaccttctg agggtcctca cagagaacga
     2581 gaccgaaaag gcccttctct tcatcgacct cgacgactac cagaacgttt gtcaggccca
     2641 cggtcacctt gttggcgacg catacctgaa gagatcggcc gaggctgtac tggaatcggt
     2701 caggtcaggc gacatcgtct cccgtttcgg gggcaaccag ttcgtagtgg tccttgacag
     2761 tggcgacctt tccctggccg agacggtggc caggaggatc aggaaggccg taggggaggt
     2821 tatccctgaa ttcaggtccg tctcggtggg tgtgaccaag attatccccg ggatcaccca
     2881 gcaggccgta ctggtgaacg cagacacagc cctctaccga tccaagcact cccccctggg
     2941 gaacatctcg atcgtggatc ctgacaaccc caggatcgag ccagagcact ggcctgaaac
     3001 tgacatgaga cttcaggaaa gataggagga agggagcccg ggaaaggccc cctcccgcac
     3061 ccttgaggga tcctcaacca gggatgaaca gaccccggat gagatcccat atccatgtga
     3121 tggccgaagc cacggccttg aagaggattc acatattatt cacccgcctt gtggccataa
     3181 taccacaggg ggtatccagg aggtagacca tgcagaataa taccttggaa gtgatccttt
     3241 ccagaagaag cataagaagg tataccgaca ggcctgttga agactcttcc atcgagaccc
     3301 tcctgaaagc cgggatgagc gcccccagcg cacacaacca gcagccctgg gagttcatcg
     3361 tcgtcgacga caggaagacc ctcgaagaaa ttcccctgtt ccacccctac tccagaatgc
     3421 ttctcaaggc tccggcagcg atagtggtat gcggtatcac cagggatatc cccgcccctc
     3481 aattctggcc ccaggactgt gcagcggcca ccgagaacat cctgctggcc gcccatgccc
     3541 tcgggatcgg atcagtgtgg ctaggggtat tcccccacga ggaacttgta tccaggctct
     3601 ctgaactgct ggctgtcccc actgacatca cccccttctc catcatctct cttggatatc
     3661 ctgacgaggg gaaggggcct tcggagagat acgacgcagc aagagtgcac agaaaccggt
     3721 ggtagcagca aagggggagg gaacccatct cgaaggaccg atcctcacag gacaaccagc
     3781 ctgttctctt tgaccccaac ctcaacatag tcttcggagt gaccctgatc tcggttctgg
     3841 gagtagccag cgttgccccg gccttcccgg agataatccg tgccctgggt gtccaccagg
     3901 aggacgcggc atggcttatc tccgcattca cgatcccggg acttctgttc actcctgtga
     3961 cgggctttct ctcagaccgt tacgggagga gaaagatcct gatcccctgc ctcttcctct
     4021 ttgggatagc aggaggggcg tgctttcttg cccgtgactt ccgcaccctg atctttctcc
     4081 ggttcctgca gggggtaggc tcggccccgc tcaacgccct gaacgtcgcc cttatagggg
     4141 actttttcaa gggcaacgac cgatcggccg ccatgggata caacacaagt gtaattggtg
     4201 taggtaccgc catctacccg gctcttggag gactgttggc catggcgggc tggaattacc
     4261 cttttctttt ggctctggtg gcccttccag ttggattctg ggcatacctt ggcctgaatg
     4321 agggagaccg gtttccacct ggcgggcaat cagcaggagg agctccccgg ccgctcctgc
     4381 gggaacttgc aagaaagggt gtcccagtcc tgctatttgc cagcatgatg accttcgtca
     4441 tccagtacgg ggggttcctc acctttcttc ccttctacct ggaatcaggc tacggcgcta
     4501 cacccctgac catagggctg gtatcggccg caatgtccat ctcgagcgct gtcacctctt
     4561 acagggcagg atggttctcc gcccgattca gtagccgctc cctgatggcg atagcattca
     4621 tactctactg ttcggcgctg cttttcatcc ttcacgtgtc gggctgttct gggatgatag
     4681 tccctgcgat gctcttcggc attggacagg ggatcaacat ccccaacctc cttacaacac
     4741 tcaccaacaa cacacctgaa gagaaccggg ccatggtgat gtctcttaac agcatgtccc
     4801 tgaggctggg gcagaccctg gggccgcccc tgttcggtat ggtcttcgca tccaggggaa
     4861 tagaggctgt attcactacc ggcgccgtca tggcaggagg tatggcggta gtgatcttcg
     4921 gaacgatgag gaagatcggt ggaacgtcag aggtgggaca tgacgcgtga cgcatgactc
     4981 gaaagatcaa aagcgtgaac ggtaaacggt caaccgtaaa cagaagtcgc agagagacct
     5041 gctttaatct tgcccttaac tgttcacgct tttttgttct tacgtctcac gtctcacgtt
     5101 tcacgatcta caaaaaagga ggtcatacca tgttctgcag ctgcaaggat atgagagcaa
     5161 ttcgaaggga gaatctccag ggaggacccg gcggtggaat gttctacccc ttgatagccc
     5221 cgggcgaaaa gcccgagggc agccacctca aaatggtggc cagagtagag ctcgatccgg
     5281 gtgcggccgt gggcgagcac acccatactg gcgacgaaga ggtctatcac atcatctccg
     5341 ggagggggat cttcatcgag gacggagtcc aggttaaggt aggcccaggg gatacggccg
     5401 ttacgcttga cgggcatagc cattccataa tcaacacagg cgacgaaccg ctggtcttcc
     5461 tggcggtgat cgctgagtaa caaaacccgt gaacagggga agcagtcact ggttactcgc
     5521 aggttaaaag gcgtaaacgc taaacagtca acggtgaacg gtcaacagta ggcatccgct
     5581 cctggccttg cggtttaccg ttcaccgggg attattttct tccccccgtt acgagttccg
     5641 agtaacaggt cctgttcttg cgtcacgtct cacgcttgat ctttttaccc tgtgctatga
     5701 tatccgaaac ctttctgggg gtgacggaaa tgatactcaa aagagccgtt cttctattcc
     5761 tcgtgctggt gtccgtcatc cttaacgcga ccgtttccac ggcttttgac gtctccgccg
     5821 tgaggggacc tcatatccgg gacctctaca tggttatcta ccgggatccc gactcccaga
     5881 tactggcgct tcaaaaggga gagcttgacg tactcggaga cgtaacccgc ccctcggata
     5941 tcgagcgact gtcgtccaac caggacgtta acctttcaat atcaagggga ttccatctct
     6001 ttttcatggg cttcaacctt cgcaagggac catggaatga caagacacta aggcaggcgg
     6061 cctcaatggc catcccgtcc attcagatcg tgagggacat cttttccgga tacagcgaac
     6121 ccgtaaacac ctatcttccc ccggtatcgc cctaccatga gaccgacgta acgacctggg
     6181 aacatgaccc caggggggca agagtgcttc tcgaggaaga aggttggagg tggtcacccg
     6241 acggaaggct gatccctccg ggggaggaat ctcccctgaa gggggtcaag atcctgtccc
     6301 ctacggccca ggtggccccc accacagcgg agatatccag caggatcgcc gacgctctaa
     6361 aggaaatagg gatccccgtc tcggtggaac ccctcgattt ctctaccatg atcagaaggc
     6421 tcgacctcca cgattttgat ctcttcgtca tggcctggag cctaaccagg gatcccgaca
     6481 gcctcttcgc ctttttccac tcctccatgg atgtgacggg agggtacaac atacagggtc
     6541 ttcacgaccc tctgctggac gaggtgctgg aagaactacg gtgggcccct gacaggcaga
     6601 ccgccgaggg agcggcccga aaggcccagc agatgctatc ggagatatcc ccgtggatac
     6661 cggtctactc acgctacatg gtggccgccg tcagaaaggg gtgggatggc gtgatcagca
     6721 cccaggccac ctcggcggac aacatctgga cccttctcaa catgaggtcc tccgatggca
     6781 gcgagagacc cgtgttctgg tgcctctccg atgaaccccg atcactaaac ccactgagta
     6841 ctggctcagc ctacgactgg caggtgctcg gtctgatcta cgatggcatg atcgccgtgg
     6901 acccggagac cctggatgat ataccctggc tggccaccaa gtgggaggtg gagacagtca
     6961 gcggagagga cggagaccat acgagactga ccttccattt gagggacagg gtcaaatggc
     7021 acgacggctc cccttttaca tcgaaggacg tgggaaagac cctggagttc ctcagggaca
     7081 acgaaatacc caggtacttc gacaacgtca gggacatcct gaaggtggag acccctgacg
     7141 acctgacggt cattgtaacg atgaagaatg tttccttctg gcatctccac aacatcggcg
     7201 gctcccctgt cttcccagcc cacctccttg aaggagttga cgaatgggag acatggcagc
     7261 ctgctgcaga accccaccct tctgtgaagg gactgacatc ccttgtgggg acagggccct
     7321 tcgtgttcag ggagtaccgc cagggagagt acgtccactt tgtcagtaac gaggacttct
     7381 ggctgatcgg aggtgactag ctcttgtggt cctactggtc ccggagggca ttgggcgcag
     7441 tagccgtact gctgctggtc atagccgcga acttcttcct tttcagggtc atgcccggcg
     7501 acgccgtatc tacgatcgtc gacccccgat tctccccgga ggccaaggaa cgcttgaggg
     7561 agctattcgg tctcgatctc cccctttacc gtcagttcct gatctatgtc cgaaggatgc
     7621 tgaccttcga tctcgggata tccttcctct cccaaagacc cgtctgggaa gaactgaaga
     7681 gccgtatccc caatacggtt gccctccttg gatcggccct gtttctttcg tcggctctgg
     7741 gaatatggct gggcgtcaag gcggccagaa gaagaggggg gttgacggaa aaacttgtcc
     7801 ttgtatcggg atcggtgtcc ttctcctttc cttcgttctt cgtccagctg gtccttctcc
     7861 tttttttcgc atacaccttc ccggtctttc ccctcagagg gagcgtatct atccccctcc
     7921 cggagggcgc tttcgcgaga gcactggatt acctgtggca ccttctttta ccggttgggt
     7981 cactggtact cctgggtttc ggctcctggg caatatacgt gaggaacctc atggtgaaga
     8041 ccctggggga agacttcatc ctcctggcca gggccagggg gcttcccgag ggcaggatac
     8101 tctggaacca tgccttcagg tcggctttgc cgccggtcct cacaatcttc ctgctggccc
     8161 ttcctggcat agtttcgggc gcagtgatca cggaaacggt attttccctc cacggcgtcg
     8221 ggaagtacct cctggattcg ataatggggc atgactaccc cgccgcaggg gcagccttct
     8281 acatccttgg ccttctgacc attgtctcca accttgctgc cgatgcagcc tacgcccttg
     8341 cggaccccag gatcagggtc gggaggaggg cccagaggtg atgaaggtcc cccgctggag
     8401 tttcataacg ttcttcctga tccttgccat agcggtcatc ggacccatga gcctttccaa
     8461 aggaccgcag gagaccgttg ccaaaccttt cgcgccaccg gtatggtccg gtggagatgc
     8521 tccgccaccc ttctccggta atctttcgcc gaactcgaaa gagatgaaca tcgaatggga
     8581 ctgggatgcc cccgaaaacc tcaccctcag gggacaactg acctttgacc atgacgttgt
     8641 ggcaagactg gtatggacga ccccggaggg gagcaagtac accctttcct ccttccatgg
     8701 aagcggggtg tggtccatcg accttgacgc gagggacatg accttcaaga cggttctggg
     8761 cattcccccc tttgccagcg cgacatccct gctctttccc tcaagggggc tccatgctct
     8821 ctcactggag ggtccttctc catcggaggg atccatggaa cttgtcctgc ccggaggaag
     8881 atgggggctt ttggggaccg accagagagg gcgagatgtc ctctcgcttt ttatcgtggg
     8941 tatcagggtg tcccttctgg tggggatctc ggccacgttc atcgcctcac tgctgggact
     9001 ctccataggg cttgcaagcg gctacctggg gggggttgcg gacagcctta tcatgaggat
     9061 cgtggatgtg cttctgtcga tccccattct accgatcctg atggtcctcg tgggaatttg
     9121 gggaaaggga ctctggcaac ttgtgctgat cctgggactt ttttcctgga tgggtacggc
     9181 aaggacggtc agatccctgg ccctctccct cagagagtcc tgttacgtag agaatctccg
     9241 ctccataggg gccgggtcct ggtacatcct ttaccgacac cttcttcctg aagctatgcc
     9301 gatagttctc gctgcggtgt ccctgggagt gccgggggca attttggccg aagcgggcct
     9361 ctcattcctc gggctttcag atcccaggat catctcatgg gggaggatgc tccatgaggc
     9421 ccacgggttc ggagccttca cggcaggggc ctggtggctt atcattccgc cggggcttgg
     9481 gatctcgatc atatgccttg tctttctcga catgggccgt tccatagaag agatggccga
     9541 tccccgactg agagggggtg gccagggctg atatccgtaa gaggtttgtc tatcacctat
     9601 cggacccccg cccgaaatgt cccggcggtc agggatgtat cgcttgagat cccggcggga
     9661 cgcataaccg ccatcgttgg gggatcgggg ggaggcaaga gcagcctgat ctatggaatc
     9721 cttcgtctcc tccctccccg gaccgaattg tcggggagca ttttcttcga cgggacagac
     9781 ctggtctcaa ccgatgagga gtggatgaga gagttcagat ggaagagagc ggccctgatc
     9841 acccagggcg cgatgaactc gcttacgccg gtcctgaccg taggctatca gatatccgag
     9901 gtactccagg tcaggctggg gctgtcgcgc agggaggccg agaaaaggac cctcgaactt
     9961 ctggcgatga cgaaccttac ccagatccat gcccgaaaat ttccccacga actctccggg
    10021 ggtgagaagc aacgggcagt catatccatg gccgtatcct gttttccggg ccttttgata
    10081 gccgacgaac cgacaagtgc actggacatg atcacccagg ccgaggtcgt cacgaccctc
    10141 gtgggaatgg tccgggatca ggggatggga ctccttctgg tcactcatga cctcccgttg
    10201 gcgatctccg tctgtgacga tctggcagtg atgtacgaag gcaggattgt ggagacggga
    10261 accccctcga agatcctgcg atccccctcc cacgaacata caaggaaact gctgtcggcc
    10321 ttcagcttca agggaggggc cagaccatga gagaactcct tctctctgcg aagggagtgt
    10381 cggtctcttt cctgtcgcca aggggcacca ccgaggccct gaagggagtg gatatggatg
    10441 ttcccaccgg cggttgcgtg gcagtggtcg gggagtcagg gagcggaaag acaacgctgc
    10501 tcagggcgtg cctgggcctg gtcccaatgt ttgaggggtc ggtgaccctt ttcgacaggg
    10561 acctgtcttt ctgtggtagc aaggaacttg taacgttacg aaggcgctgc ggctacgtcc
    10621 cccaggaccc cttcggatgc gtcccaccca cgctgaatac ccttgatgcc gctgcaga
//
LOCUS       DAOPKQ010000020        10660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_6508_length_10660_cov_4.649316, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000020 DAOPKQ010000000
VERSION     DAOPKQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6508_length_10660_cov_4.649316"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK146_01465"
     CDS             <1..389
                     /locus_tag="PK146_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90936.1"
                     /translation="GVCDGWIGGSSQINYLSFRDVIKYYIPYNCLFDQTGYIIGKDIW
                     NKISPEDQKVILDAVNEQADKSFADSNAEDVEYQEKLAEAGVEIIPISGSEMAELAEY
                     IRNTTWPKLESTYGKETLDKIKESLQ"
     gene            552..971
                     /locus_tag="PK146_01470"
     CDS             552..971
                     /locus_tag="PK146_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE90937.1"
                     /translation="MCAEVVLRYVFKSDLFGIEEIVVIAAFWLYFMGSSYGVFEKSHV
                     KADIIPQMLRPRARALLSVLVNFTVSALCILFTVWAVGMVRYSITWMPRTTGLRIPIY
                     ISQSSVLLGYALMSMYSIVYFIEDLFVFVSMGREREA"
     gene            1065..2369
                     /locus_tag="PK146_01475"
     CDS             1065..2369
                     /locus_tag="PK146_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE90938.1"
                     /translation="MAITVALLLIIITLLLGLPVPFCFMLSTTFMVVTHGYDPSFLLP
                     YGFSQMSSIVLLAIPLFIMVGGMMDRGGIGTSLVNFVEIFVGRIKGGLGVVAVVSCAV
                     FGSISGSCAATLSCIGSIMFPRLREKGYPMGHSAALVSCAAPLGLLIPPSSQMILYAW
                     VGQQSVLACFLATVGPGIVLMILLSLLNLIMLRKDPNIEVVPVMSRGEKLSMFRDRGK
                     RAAPALFMPFIVLGGIYGGIMTPTEAAAMAVLYSIPVGFWVYRGLNRGNFIEVIIETA
                     TTTGVVMLMMYCIMMLSRIYVQERLPDMIINTLTAVSTNRYVILFMINIFMIIIGMIM
                     DDVSAMLLCTPILLPVVTALGVHPIHFAAIIGTNLGMGNVTPPTAPTLYFGGRMAKTP
                     VSQMMKPALVFITFAWLPTLLAVTYLPELALWLPRLVLGGRI"
     gene            2381..3589
                     /locus_tag="PK146_01480"
     CDS             2381..3589
                     /locus_tag="PK146_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HPE90939.1"
                     /translation="MGKMDYLQEKPIAVLGGGATARGHAACAALAGREVRLYELPDFF
                     EGLGCIKENREIRLSGIQESLYGFKREGLAKIDVVTSDMEEAVKGAGIIVVSFPAVGY
                     KAFLEKLIPRLEDGMVVHFTTANFGSLIMRKMMRESGCSKRIVIGEWSSQPYGIRIKS
                     AGGQQMPEVSVNYWAISLRGSALPMIDQEKFFESKQYLPSLDSVVHPVRGDTVADIGF
                     SNVNPILHCPGTILGVGTMENWGVIYGGDKHDFSIYSHAYCPSISKVQLALYKEECAI
                     AEAMGVGIQQFSEESFFSRSNILGSEHMGGKFKVPFDEQYKLALGTGPFSIYNRYITE
                     DIPVGCHIFRELGKKFGVKVPVIESMITLASVMTGVDYWSEGVTLNDLGIEHMDREAL
                     NAYLREGTYL"
     gene            3673..4887
                     /locus_tag="PK146_01485"
     CDS             3673..4887
                     /locus_tag="PK146_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE90940.1"
                     /translation="MEWQGLFNGSATNRLVDSAIASIMKIASEENAMSLAAGEPFGAL
                     YPAEELRDCLMISFSKELPVWGYSNSRTGIAPLRQWIADWMARDGLLPQWANSDSLFI
                     TNGSQEGLYLLCECLLEPGDTVIVESPSYPEAFGIFRKFGAKCLSVPLSEEGPDIGVL
                     EGILKENRVKFFYTIPTYQNPTGFSTPDSKKQSVLDLALRHGFVIIEDDPYRNLSFEG
                     RPEGTYIRAAGDKKNVAYLGSFSKIVAPGLRCGWVVAPQPVIEKMLKIRIMGTLCLPE
                     ILHYGLWEFVNSIDLTEYLEGLSRTYRQHRDALVSSLMRHAGPHGLELAVPKGGFFLW
                     GRVPWIEDMKSFALYAIREEKIAVIPGKEFFLGGHVEEDTVRLSFSKVTPSMAEECAR
                     RLGTALKRFRDL"
     gene            4931..6586
                     /locus_tag="PK146_01490"
     CDS             4931..6586
                     /locus_tag="PK146_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE90941.1"
                     /translation="MGDHSKRELYGLLLNLVGIPSVSPSAESENRIARFIHDSFQDLS
                     YFQENRGDLRLLPLEGDPLGRHLVFAIVRARKRTADTVILMGHMDVVGTEACGFLAPL
                     AFSPEEYTERIASVAIPRDARKDLESGEWLFGRGVADMKSGVAAGMDLLMEAAENPGK
                     YDANVAVLFVPDEENNSGGMLSGSSCLARFQKEESLRYLACIDLEPTFATGDDARPTI
                     YLGSIGKINPFFFCAGKETHVGEYYEGFCAAPVISRINLMLDGNPEYADSLFGKSYPP
                     FGCMRQMDLRREYSATIMTKAFAFYSYLTSTRLPGQILSEMRGIAMEALEASIEKYRE
                     NTSSFAAMGGTTSKPRDWEPTVVSFDELSDMAEKVLGDGYARFMDETVGSAPEGSDER
                     VRAALLVESMVELCGIPGPMAVIGFLPPWYPHRSNLGETRGEKVVADAASATAGEMER
                     RFGLPLDIRPFFEGVSDLSYCGFQGDSSEMEVFARNMPGWGRPYRIPVEALAELDIPI
                     LNLGPVGKDAHKNTERIHLPFMLEVFPNILRFVVERIIEGYRS"
     gene            6703..7602
                     /locus_tag="PK146_01495"
     CDS             6703..7602
                     /locus_tag="PK146_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPE90942.1"
                     /translation="MTLWLKRILGRVPELRPSPGVLKEWGMIHLGCLLVAAGITWFLV
                     PNNIAAGGVSGLAMVLNRFLPGVPVGVLMMGMNVVLFVIGFIFIGFGFGAKTIYSSLF
                     LSGAVWLLERFYPVSGPLTEDIMVQLVFGTILGATGMAIVFNQNASTGGTDITAKILN
                     KYLNIDIGKGVLLCDFFIVLAAGWVFGIRTGMYAMLGVILNGFLVDSVIVGLRINKYV
                     VVISRQSEAIRRFIVEELESGATIYEGRGGWSGEPKEVITTIVNRRKFIRLRNFIKTV
                     DPRAFISVNNIHEVLGEGFERLA"
     gene            7651..8406
                     /locus_tag="PK146_01500"
     CDS             7651..8406
                     /locus_tag="PK146_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPE90943.1"
                     /translation="MRLEDRPVRLVLYNTRYGTGTGWAYHAPFPFAGTLKTTRERSSS
                     IARFIASLEPDIVGLVETDGGSFRHGGRSQPEYLAETLGHSWVFSSKYCIPRISGCVP
                     VFRHQGNAVVTSLPIRETSVRRLRRGIKNAVIETGFDDFDLKLVHLSLGSGARRQQIL
                     ELAAMARERKRPLLIAGDFNAFGGGPEIEPLIEAGLMNADTLGIRTYPSGRPRLGLDL
                     VLHSPEIDVRRIEVPDVRLSDHLPVVCDFALSL"
     gene            8429..9883
                     /gene="cls"
                     /locus_tag="PK146_01505"
     CDS             8429..9883
                     /gene="cls"
                     /locus_tag="PK146_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPE90944.1"
                     /translation="MDLSLLLILIHDLSSVSIRLATIALIPRWHSPSVAMAWLLVIFF
                     WPIPGLVLYLVFGSFKLPAQRAERHEKILKDLDRTCCAAWEAERPEEKDLPGDLLRLL
                     RLASLAEKLGDMPPTRGNTIEIIDSTDDMVRSLASDIDTARHHVNLLYYIFSKDTVTG
                     PIFDALERAASRGVSCRLLVDSLGSRQFLKRDADSLRAAGVHVVGALPLSMFRRNPLS
                     ARFDLRNHRKIAVIDGRTGYMGSHNMIEPSYGGKARGLLWKDLTMKVEGPESLQLQRV
                     FLEDWYVETMELLDDRAGDILFSHHGEHILQTVPSGPSYRTENYQRLVVSALHSASRE
                     VVITTPYLIPDESLMEGLEVAVLNGATIKLIIPRRSDQFFVGKAAKAYYDILLDKGVR
                     IFLYGDGVIHAKTMSVDGDLCFFGSSNFDIRSFALNFEINCVIYGKNSVARILQEQEK
                     YLEASTELFMEEWGERPFLFKAVEGVAKLMSPLL"
     gene            9899..>10660
                     /locus_tag="PK146_01510"
     CDS             9899..>10660
                     /locus_tag="PK146_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90945.1"
                     /translation="MSREKVLLLFDEERPFWTERLIGMAGEDPAVAVSLAREGLLKSL
                     GRGFCLSEEGQIVFCRWAAESYLESLPGGEPGDLELEELKLETALLFERGFKGFQGTK
                     RVIVSPRLEYFPGVPPAEIFSISEGSIQWRFFEHPLVAKLVSSFPRERSAEGESLQEL
                     ERRVNSMDANRVHWSPHILCINQCDYAYYWRSRLETDRWGLLNADRLFLMLVRDPENF
                     DLEKAASDMAAFSMFLLDNRHVYLPGCFDIDTQQQS"
BASE COUNT         2307 a         2828 c         3087 g         2438 t
ORIGIN      
        1 caggggtctg cgacggttgg ataggaggat cgtcacagat aaactatctc agcttccgtg
       61 acgttataaa gtattacatt ccctacaact gcctcttcga ccagacaggc tacataatcg
      121 ggaaggatat ttggaacaag atctctcccg aggaccagaa ggtcatcctt gacgccgtta
      181 atgaacaggc cgacaagagc tttgccgaca gtaacgctga ggacgtcgag taccaggaga
      241 aactcgctga agccggtgtg gagataatcc ccatctccgg aagtgaaatg gccgaacttg
      301 cggaatacat tcgcaatacc acctggccca agctggagag cacctacggc aaggaaaccc
      361 tggacaagat caaggaatcg cttcaataga ccatcccttt cgccgggcag gttcacaacc
      421 tgcccggcgt ttctctggag gggtcaaatg tgaatctcat tggatcgatc aaaaggtcgc
      481 ttttctggaa gtcgatcttg ggtctgcaac gtattctctt ggtactaacc agccttttcg
      541 ttgtgctgat aatgtgtgcc gaggtggtcc tgagatatgt gttcaaatca gatcttttcg
      601 ggatcgagga gatagtggta atagcagctt tctggctcta tttcatgggc agttcctacg
      661 gggtcttcga gaagagccat gtcaaggccg atatcatccc ccagatgctg agacccaggg
      721 caagagcttt gctcagcgta ctggttaatt tcaccgtgtc ggccctctgc attctgttca
      781 cagtatgggc tgtagggatg gtccggtaca gcattacctg gatgccaaga acaaccgggt
      841 tgaggatacc catctacatt tcccaatctt cagttttgtt ggggtatgcc cttatgtcca
      901 tgtattcgat cgtctacttc attgaagatc tctttgtatt cgtttccatg ggccgggaga
      961 gggaggcctg atatgagatc tgtcatctgt tcccgttccg ccaagcacga tcgtgacaat
     1021 gatcctgcag accgcgttag gacaggaaat aggagggaat gggcatggcg ataaccgttg
     1081 cccttttgct gattattatt acccttcttc tcgggctgcc cgttcccttc tgcttcatgc
     1141 tctccaccac cttcatggtg gtaacccacg gttacgaccc atctttcctc ttgccatacg
     1201 ggttcagcca gatgagctcc atcgtccttc tggctatccc gttgttcata atggtgggcg
     1261 gaatgatgga caggggggga atcgggactt cgctggtgaa ttttgtggaa atttttgtgg
     1321 gacgcatcaa gggcggtctg ggggttgttg ctgtcgtttc ctgtgccgtg ttcggttcga
     1381 tctccggaag ctgtgcggcg accctttcct gcattgggtc gatcatgttc ccgaggctca
     1441 gggaaaaggg ctaccccatg gggcacagtg cagcccttgt ctcatgcgct gccccgctgg
     1501 ggcttttaat tccacccagc tcccagatga tcctctacgc ctgggtcgga cagcagtcag
     1561 tgctagcctg tttcctcgcc acggtggggc ccggtattgt tctgatgatc ctgttgagcc
     1621 ttctgaatct gatcatgctt cggaaagacc cgaacataga ggtcgttcct gtcatgtcca
     1681 ggggagagaa gctttccatg ttcagagatc ggggcaaaag agccgcgccc gccctcttca
     1741 tgccctttat tgtgctcggt ggtatatacg gcgggataat gaccccgaca gaagcggcag
     1801 ccatggcagt cctctactcg atacctgttg gattctgggt ttacagaggt cttaaccgcg
     1861 gcaacttcat cgaggtcatc attgagacgg ccactacgac gggggtcgtc atgctgatga
     1921 tgtactgcat aatgatgttg agcaggatct acgttcagga gagactcccc gatatgatca
     1981 tcaataccct cacagccgtc tctacgaaca ggtacgtcat tctgttcatg atcaacatct
     2041 ttatgataat catcggtatg atcatggacg atgtgagtgc catgctactt tgcaccccta
     2101 ttctgcttcc ggtcgtcacc gcactgggag ttcacccgat ccatttcgcg gctatcatcg
     2161 gtaccaatct ggggatgggt aatgtaaccc ctcccaccgc tccgacactg tattttggtg
     2221 gccgtatggc gaaaaccccg gtctcgcaga tgatgaaacc tgccctggta tttatcacct
     2281 ttgcgtggct gccgacactg ctcgctgtaa cctacttgcc cgaactcgct ctctggcttc
     2341 cgcgactggt tctagggggc cgtatatagg aggttaaact atggggaaaa tggattatct
     2401 tcaagaaaaa ccgatcgctg tcctcggagg aggcgctact gcccgtggcc atgcggcatg
     2461 cgcggccctt gccgggcgtg aggtcaggct ttatgagctc ccggattttt tcgagggact
     2521 ggggtgcata aaggaaaacc gtgagatccg gctcagcggg atccaggaaa gcctgtacgg
     2581 gttcaaacgg gaaggcctgg caaagatcga cgttgtgaca tccgatatgg aagaggctgt
     2641 aaaaggggca gggataattg tcgtcagttt cccggcagtc ggctacaagg ccttcctgga
     2701 aaaactgatc ccccgcctgg aggacggaat ggtggtacac ttcaccaccg cgaatttcgg
     2761 gtcgcttata atgcgaaaaa tgatgaggga gtccgggtgc agtaaaagga tcgtaatcgg
     2821 cgagtggagc agccagccct acgggataag gatcaagagc gccgggggac agcagatgcc
     2881 tgaagtgagc gtaaactact gggccatttc cctgagaggt tctgcccttc ccatgataga
     2941 tcaggagaag ttcttcgagt caaagcaata tcttccttct cttgattcgg ttgttcatcc
     3001 cgtcaggggt gacaccgtcg cggacatagg cttcagcaac gtgaacccga tcctccactg
     3061 cccagggact atcctcggcg tcgggaccat ggagaactgg ggcgttattt acggagggga
     3121 caagcacgat ttttccatat actctcatgc atactgcccc tctatctcaa aggtgcagct
     3181 ggccctgtac aaggaggagt gcgccatcgc ggaagcaatg ggagtgggta tccagcaatt
     3241 ctcagaagag tcctttttct caaggtccaa cattctcggt tcggaacaca tggggggaaa
     3301 attcaaggtc cccttcgatg aacagtacaa actggctctg ggaaccggcc ctttttccat
     3361 ttacaatcgc tacatcactg aggacattcc tgttggctgt cacatattcc gggagttggg
     3421 gaaaaagttc ggagtgaagg tcccggtcat agaatcgatg ataacccttg cgtcggtaat
     3481 gaccggagtc gattactggt cggaaggggt taccctcaat gacctgggaa tagagcatat
     3541 ggacagggag gcacttaacg cctaccttcg tgaaggaaca tatctttaac ttcgaggggc
     3601 ttcttcgaag atgaaggagc ccctcggaat tttccgtggt ctgatctttc aagttaggag
     3661 agtaagaacg agatggaatg gcaaggactg ttcaatggat ctgctacgaa ccggcttgtg
     3721 gattccgcga tcgcttcgat catgaagatc gcctcggaag agaacgccat gtccctggct
     3781 gccggcgaac ctttcggggc tctctacccc gcggaggaat tgagagactg tttaatgata
     3841 tctttttcaa aggagctgcc tgtctgggga tacagcaact cacggacagg gatcgcccct
     3901 ttgcgccagt ggatcgcaga ctggatggcc agggacggtc ttcttccaca atgggcaaat
     3961 agcgacagtc ttttcattac caacgggtcg caggaagggt tgtacctgtt atgcgaatgt
     4021 ctcctggagc cgggggatac cgtgatagta gaatcgccat cctatccgga agcgtttggg
     4081 attttcagga aattcggggc aaaatgcctg agcgtacccc tttcagagga aggtcccgat
     4141 ataggggttc tggaagggat tttaaaggag aaccgggtca agttctttta caccatccct
     4201 acctaccaga atccaactgg tttttcgacc cctgacagca agaaacagag cgtactggac
     4261 cttgctctca ggcacgggtt cgtgataatt gaggacgacc cttaccgcaa cctttccttc
     4321 gaaggtcgac ccgaagggac ctatatcagg gccgcaggag ataaaaagaa cgtagcctat
     4381 ctcggtagct tttccaagat cgttgcaccc ggccttcgat gtgggtgggt ggtcgctccc
     4441 caaccggtaa tagagaaaat gctcaagatc aggatcatgg gaacgctctg tctcccggaa
     4501 attttgcatt atggcctctg ggagttcgta aatagcatcg atctgacaga atacctcgaa
     4561 ggtctttcaa ggacctatcg gcaacaccgg gatgccctgg tctcctccct gatgagacat
     4621 gccgggcccc atggacttga gttggccgta ccgaagggag gattcttcct ctggggtagg
     4681 gtgccctgga tcgaagacat gaagagtttc gcgctgtatg ctatcagaga agagaagata
     4741 gcggtgatcc ctggaaagga gtttttcctg ggaggacatg ttgaggagga tactgtcagg
     4801 ctctctttct cgaaggtcac cccttccatg gctgaagagt gcgctcgtcg cctcggcacg
     4861 gctttgaaga gattccggga tctttgatac ttgcctgaat gtcctttccc gaacgacagg
     4921 aggatgctgt atgggtgacc attcaaaaag agagttgtat ggcctgcttt taaacctggt
     4981 cgggattccg agcgtctctc cctccgcaga gagcgagaac aggatcgccc gtttcatcca
     5041 cgacagtttt caggatctga gctattttca ggagaaccgg ggcgatctac gtcttctgcc
     5101 gctggagggt gacccccttg gaagacacct ggtcttcgcc attgtaaggg cccggaagag
     5161 aacggccgat acggtgatcc ttatgggaca catggacgtg gtgggaaccg aggcgtgcgg
     5221 ctttctggcg cccttggcct tctcccccga ggagtacaca gaacggatag cctcggtggc
     5281 gatcccccgg gatgcccgca aggacctcga gtccggcgaa tggctcttcg ggaggggggt
     5341 ggctgacatg aagagcggtg tcgccgccgg gatggacctg ctcatggaag cagccgagaa
     5401 ccccggtaaa tatgacgcca acgtggctgt tctcttcgtt cccgacgagg agaacaactc
     5461 tggcggtatg ctttcgggtt catcctgcct ggcccgtttc cagaaagagg agagtctccg
     5521 gtacctggcc tgcatcgacc tggagcccac cttcgccacc ggggacgatg ccaggccgac
     5581 catatatctg gggagcatag gcaagataaa cccctttttc ttctgcgctg gcaaggaaac
     5641 ccacgtgggg gagtattacg agggtttctg cgccgcgccg gtcatttcga ggatcaacct
     5701 gatgctcgac gggaaccccg agtacgcaga ctccctcttc gggaagtcat acccaccctt
     5761 cggctgcatg aggcagatgg acctccggag ggagtactcg gcgactatca tgaccaaggc
     5821 tttcgccttc tacagctacc tgacgtcaac caggcttccg ggtcagatcc tgtcggaaat
     5881 gagggggatc gccatggagg ctctggaggc ctcgatcgaa aagtaccggg agaacaccag
     5941 ttcctttgcg gcgatgggtg gcaccacctc gaaaccccgc gactgggagc cgacggtggt
     6001 ctccttcgac gagctttcgg acatggcgga gaaggtcctg ggcgacgggt atgctcgctt
     6061 catggatgag acggtcggtt ccgcccctga gggttccgac gagagggtcc gtgccgcact
     6121 ccttgtcgag tccatggtgg aactctgcgg gataccggga ccgatggcgg tcataggttt
     6181 tctccctcca tggtaccccc acaggtccaa cctgggagag acccggggcg agaaggtggt
     6241 tgccgatgcc gcttccgcga cagccgggga gatggagagg aggttcggcc tcccccttga
     6301 tatcaggcct ttctttgagg gagtctcgga cctcagctac tgcggcttcc agggtgacag
     6361 cagtgagatg gaggtcttcg ccaggaacat gccagggtgg ggtagacctt acaggatccc
     6421 tgtcgaagca ctggctgaac tggacattcc gatactgaac ctgggacctg tgggcaagga
     6481 cgcacacaag aacaccgaac gcattcacct tccatttatg ttggaggttt tcccgaatat
     6541 cctgcgtttc gtggtggaaa ggatcatcga agggtaccgc tcctgatcgt cattgccgga
     6601 taaggaatgc gggggtcccc tgaaggggac ccccgtcctg tgttacaata cctgttcccc
     6661 tcaagagggt gagatccaga aaaggaaagg gtgatgcaaa ccatgaccct ttggctgaaa
     6721 aggatacttg gacgtgtccc ggaactccgt ccgtctcccg gggtcctgaa agagtggggg
     6781 atgatccacc tcggctgctt gcttgttgcg gcggggatca cctggttcct agtgccgaac
     6841 aatattgccg ccggaggcgt cagcggcctt gccatggtcc ttaaccgctt cctccccggg
     6901 gtcccggtgg gggtactcat gatggggatg aacgtggtcc tcttcgtcat agggttcata
     6961 ttcatcggat ttggattcgg ggcgaaaacg atctattcga gccttttcct ttcaggggct
     7021 gtctggttgc tggagaggtt ctacccggtg tctggaccac tgacggagga catcatggtc
     7081 cagcttgtct tcggaaccat cctgggggcc acgggaatgg ccattgtctt caaccagaac
     7141 gcctcgacgg ggggcacgga catcaccgcc aagatcctaa acaagtacct gaacatcgat
     7201 atcggcaagg gtgttctctt gtgcgacttc ttcatagtgc tggctgccgg ttgggtcttc
     7261 gggatccgga caggtatgta tgcgatgctg ggggtcattc tcaacggatt cctggtggac
     7321 agcgtgatcg taggactcag gatcaacaag tacgtggtgg tgataagccg tcagagcgag
     7381 gcgataaggc ggttcatagt cgaggagctc gagagcggtg ccaccatcta cgaggggcgg
     7441 ggaggatggt caggggaacc gaaggaggtc attaccacca tagtgaacag aagaaagttc
     7501 atcaggctcc gcaatttcat caaaaccgtc gatcccaggg ccttcatcag cgtcaacaac
     7561 atccacgagg ttctgggtga aggttttgaa aggctggcct gaagggtcca tcgataacaa
     7621 tattcaggtt acaatctccg aaggggggtg atgcggctgg aagaccgtcc cgtaagactt
     7681 gttctctaca atacccgata cggcactgga acggggtggg cctaccatgc ccctttcccc
     7741 tttgccggaa cgttgaagac caccagggag cgctcctcct cgatcgcaag gttcattgcc
     7801 tccctcgagc ctgacatcgt cggactggtt gagaccgatg gaggatcctt caggcacgga
     7861 ggaagaagtc agccggagta cctggcggag accctgggac acagctgggt cttctcatcc
     7921 aagtactgca ttcccaggat ctccggatgc gtccccgttt tccgccacca gggaaatgcg
     7981 gtggtgacgt cccttcctat ccgggagacc tcggtccgca gactgaggag gggcatcaag
     8041 aacgcagtga tagagaccgg tttcgacgac ttcgacctga agctggtcca cctgtcgctg
     8101 ggttccggcg ctaggcggca acagatcctc gagctcgcgg caatggccag ggagcgtaaa
     8161 agacctcttc tgatagcggg ggatttcaac gccttcggag gcggtcccga gatcgagccc
     8221 cttatcgaag ctggtcttat gaatgccgat acccttggca tacggaccta tcccagcggc
     8281 aggcccaggc tgggcctgga tctggtactg cattcgccgg agatagatgt caggaggatc
     8341 gaggtgccgg atgtgcgtct ctcggaccac cttccggtgg tctgtgattt cgccctgtcc
     8401 ctgtagtgct ttccggaggt gaccggagat ggacctgagc ctgcttctga tactgatcca
     8461 cgacctctca tcggtatcca taagactggc gaccattgcg ctgataccac ggtggcactc
     8521 gccctcggtg gccatggcgt ggctgctggt catcttcttc tggcccattc ctgggcttgt
     8581 gctctacctc gtcttcgggt ccttcaagct tcctgcccag agggccgaaa ggcacgagaa
     8641 gatcctgaag gatctggacc gcacatgctg tgccgcctgg gaggctgaaa ggcccgagga
     8701 gaaggacctc ccaggggatc ttctccgcct gttgcgcctg gcttccctgg ccgagaagct
     8761 gggagacatg cctcctacca ggggcaacac gatagagata atcgactcta ccgacgatat
     8821 ggtcaggtct cttgcctccg atatcgatac cgcccgtcat cacgtgaacc tgctctacta
     8881 catcttctcc aaagacacgg tcacaggtcc gatcttcgac gcacttgaaa gggcagcctc
     8941 aaggggcgta tcctgccgcc tgctggtaga ttccctcgga tcaaggcaat tcctcaaaag
     9001 ggacgctgac tctttgcgcg cagcgggggt ccatgtggtg ggcgccctgc ccctgagcat
     9061 gttcaggagg aaccccctct cggcccgttt cgacctcagg aatcaccgca agatcgctgt
     9121 tatagacgga aggacgggtt acatgggttc ccacaacatg atcgagccct cttacggggg
     9181 gaaggccagg gggcttctat ggaaggatct gacaatgaag gtcgaagggc ccgagtccct
     9241 gcagctgcag agggtctttc tggaagactg gtacgtggag accatggaac tccttgatga
     9301 tcgggccggc gacattcttt tcagccatca cggagagcat atcctccaga ccgtgcccag
     9361 cggtccatcc taccgcaccg agaactacca gcggctggtg gtctcggccc tgcactccgc
     9421 ctccagggag gtagtcataa ccacgcccta cctgataccc gacgagagcc ttatggaggg
     9481 tctggaggtg gcggtcctca acggggcgac catcaagctg atcatcccca ggaggtcgga
     9541 ccagttcttc gtgggcaagg cggccaaggc ctactatgac atcctcttgg ataagggagt
     9601 aagaatattt ctctacggcg atggcgtcat ccatgcaaag accatgagcg tcgacggcga
     9661 cctctgtttc ttcgggtcca gcaacttcga catcaggtcc tttgccctca acttcgagat
     9721 aaactgtgta atatacggga agaattccgt ggcaaggata ctccaggagc aggagaaata
     9781 cctcgaggca tcgacggagc tgttcatgga ggagtgggga gaacgcccct tcctcttcaa
     9841 ggctgtcgag ggtgtggcga agcttatgag ccctctcctt tagaaaggag tgaaggactt
     9901 gagcagagaa aaggtactcc tgctttttga tgaagaaaga ccattctgga cagaacggct
     9961 gataggaatg gctggcgagg accccgccgt agctgtcagt ctcgctaggg aagggcttct
    10021 taagagcctt ggaagaggct tctgcctgag tgaggaaggg cagatcgtgt tctgccgatg
    10081 ggccgccgaa tcctaccttg agtccctccc tggaggagag ccaggtgacc ttgagctgga
    10141 ggaacttaaa ctcgagacgg cgctgctctt cgaaaggggt ttcaagggct tccagggcac
    10201 caagagagtg atcgtatcgc caaggctgga atacttcccc ggcgtcccgc ccgctgagat
    10261 cttttccatt tcggaaggat ccattcaatg gcgcttcttt gaacaccccc tggtggcgaa
    10321 gctggtctcc tcctttccca gggagcgttc agcggaagga gaaagtcttc aggagctgga
    10381 gcggagggtt aattctatgg acgccaacag ggtccactgg tcaccccata tcctctgcat
    10441 caaccagtgc gattacgcct actactggag aagccgcctc gaaacggacc gctggggtct
    10501 tcttaatgcg gaccgcctct tcctgatgct ggtcagggac ccggagaact tcgaccttga
    10561 gaaggccgct tccgatatgg cagctttctc catgttcctc ctcgacaacc gccatgttta
    10621 cctccccggc tgtttcgata tagatactca gcagcagagc
//
LOCUS       DAOPKQ010000021        10356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_6771_length_10356_cov_5.447821, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000021 DAOPKQ010000000
VERSION     DAOPKQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6771_length_10356_cov_5.447821"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1025)
                     /locus_tag="PK146_01515"
     CDS             complement(99..1025)
                     /locus_tag="PK146_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPE90946.1"
                     /translation="MDSFLMVLPLALVMGLGVLFRRIGLLDGRAVHRLNSILYWAALP
                     ALLFRSILGVGPGILSEPNLFWAVHSTFLIVPAIALILARISTSDRRRLAVSVLASIR
                     SNNVFMGIPAVTIAMGQEGFQAVSLYLAVSLVGFNFISITWGQVILSGRLTLKSLHDT
                     LLQLLKNPLILASIMGMAGAFAGFNRLPFWLDSAVKMVGDTGSGIALIALGASISFEN
                     LRGAMKSAWRDSLFKLFVNPALVWCAFLAWPVDPVLRNAVVLTSAMPAAVNSFVIAHK
                     MGMDHEYAGKIVALSTALSVFSLPIWIWLLGI"
     gene            complement(1120..1467)
                     /gene="rplS"
                     /locus_tag="PK146_01520"
     CDS             complement(1120..1467)
                     /gene="rplS"
                     /locus_tag="PK146_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPE90947.1"
                     /translation="MDSRIALIESRYLRKETPEFRPGDTVKVHVKVTEGNRERIQVFE
                     GIVIARKHGGMRETFTVRKVSGGVGVERVFPLHCPSVDRIEVVRHGRVRRAKLYYLRK
                     LSGKAARIKERRF"
     gene            complement(1504..2667)
                     /gene="trmD"
                     /locus_tag="PK146_01525"
     CDS             complement(1504..2667)
                     /gene="trmD"
                     /locus_tag="PK146_01525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPE90948.1"
                     /translation="MIVTVITPFPELLGPFFSTGVIGKAVEKSLIDIRFINPRDHSDG
                     HYGQIDDYAYGGGGMVLMAEPLARAVDEAKAGGQAFVVHPGPQGVVLGQHLVESLASK
                     EHLVIVCGRYEGVDERFVDKYVDLEISIGDYVLTGGELPAMVIIDAVARLVPGVVGQE
                     AAVEEDSFFRGMLDTPHYTRPSSWKGSEVPRVLTGGDHLKTSSWRRRQTVARTLGRRP
                     DMIARANIGPYLDKGVYVILVPARREVTLEEAEAIADLGASFGCERAIFISTDEAFHK
                     ELQESLGGKGKVLRSPGRAIAWIKDREKEDPFVIICGSTDRGSIHWLEAKRLGLASGR
                     PIVFLAGEGAESFTPDPGQQVSLGPVRGGRDDRTLSCPRDTLAVILDRFFGRR"
     gene            complement(2664..3197)
                     /gene="rimM"
                     /locus_tag="PK146_01530"
     CDS             complement(2664..3197)
                     /gene="rimM"
                     /locus_tag="PK146_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200414.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPE90949.1"
                     /translation="MKGPTMVTIGRILGAHGVRGEIKVLPLTDFPERFLDMDRIDIFR
                     PEGKLIASLSIRTIRLHEGKGLFLVGSEDISDRDKAQALEGGLIMVDPSERVPLPEGS
                     YWIEDIVGLAVVLSGTGEELGKVEDVLRTGVHDVYSLRTPEGDVRMIPAVREVVLRVS
                     PEEGVIEISLPEGLWDR"
     gene            complement(3208..3453)
                     /locus_tag="PK146_01535"
     CDS             complement(3208..3453)
                     /locus_tag="PK146_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPE90950.1"
                     /translation="MPDYEELVGFIVRSIVSDPDGVTITSQRSVGGVVKVLVRVSRDD
                     MGRLIGKHGVTINAIRMVARAAAVKPGEKVEVDILED"
     gene            complement(3466..3735)
                     /gene="rpsP"
                     /locus_tag="PK146_01540"
     CDS             complement(3466..3735)
                     /gene="rpsP"
                     /locus_tag="PK146_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPE90951.1"
                     /translation="MAVRIRLARFGRKKQPFYRLVVADSRAPRDGAFIEQIGSYDPMK
                     EPAALKVQEDRALYWLGVGALPSDTARAILKKTGVWDKFTSGKKQ"
     gene            complement(3821..5158)
                     /gene="ffh"
                     /locus_tag="PK146_01545"
     CDS             complement(3821..5158)
                     /gene="ffh"
                     /locus_tag="PK146_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPE90952.1"
                     /translation="MFDSLRERLEGVFKGLRSRGKLTEEDISVALRDVRRALLEGDVN
                     FRVVKDLVDRIRERAAGRDVLDSITPAQQVIAIVYEEMAALMGSGRQSLSFSPKPPTK
                     YLLVGLQGSGKTTTCVKIAKKISDGHKPLVVGCDLRRPAAVDQLRILAGQAGVGFFGP
                     SGGEKDVLPLVRRAEEYAARRLYDVILFDTAGRLNIDDQLMEELASLGGILSPTETLL
                     VVDSMTGQEAVNVAAAFNDKIPLSGIVLTKLDGDSRGGAALSIRAATGVPVKLAGVGE
                     KVTDIEQFDPRRMAQRVLGMGDVQGLVERIQEAADDREIEKIAASVKKKRMTMDDMLL
                     QIRQVRKMGPLDKVLEMIPGAGRVKGLDNVQLDPGRLAHVEAVILSMTKKERENPQII
                     KGSRRRRIAQGSGTSVQMVNQVLKQFEQMSMLMKRFAPGKGAKLPRGFPPIPM"
     gene            complement(5167..5523)
                     /locus_tag="PK146_01550"
     CDS             complement(5167..5523)
                     /locus_tag="PK146_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPE90953.1"
                     /translation="MKDQIEHRVMLSRLFDFYEPVLTEKQREAFRLHVLEDWSLSEVA
                     ERLEVTRQGAHDLVQRGRDRLLEMEGLLGFSAKDAKWERHFRELGRWYQRHMEKISQE
                     AAAELAVLLAPEEREG"
     gene            complement(5834..6109)
                     /locus_tag="PK146_01555"
     CDS             complement(5834..6109)
                     /locus_tag="PK146_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90954.1"
                     /translation="MCRVITPDQSGGHAHVDDPLSIAELLRETLSQELDAMTELAARY
                     HIIEDEEIRHSLGYLIGSRRKDLAVLWDLLQKAEEKAFGGHHHGHEH"
     gene            6270..6932
                     /locus_tag="PK146_01560"
     CDS             6270..6932
                     /locus_tag="PK146_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPE90955.1"
                     /translation="MNPMQKKNLEVRRTYNEHLGKSVVKALEANGFKAVYAGSSEEAV
                     NKVMELVPEGSSVGVPGSSTIRELGLFEELQARNCRVFEHWDPSLAPEDRSRRWEEEN
                     TSDVFLSSSNGVTLDGMLVNIDGTGNRVAAMAWARNRIIFVVGINKVSRDIQSATQRI
                     RDVATPLNAISKGIDIPCANTGFCVNCKVPNRFCRATLVLERAPFGRDVHVIIVGEEL
                     GY"
     gene            complement(7118..8569)
                     /locus_tag="PK146_01565"
     CDS             complement(7118..8569)
                     /locus_tag="PK146_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPE90956.1"
                     /translation="MRLAVVAKVLSLLCLLVSLFMVFPMIWSLADGSNDLPALSGGMA
                     TGLLVSAVLFAVGFKAKARDLGVREAFAVVSLSWVFASAIGGVPYLLNGTVSSFTDAF
                     FEAMSGFTTTGASILTDIQSNPRGILFWRDLTHWLGGMGIIVLSLAILPFLGVGGMQL
                     YKAEVPGPVPEKITPRVRQTALLLWGVYVLLSAIQVGALMLGGMDLFESLTHTFGTMA
                     TGGFSPLNGSIGQYGNPYFEWVITLFMFLAGANFVLHYLALKGDLGAWWRDEEFRFYA
                     KITVASVLAATAVLFATGTYGTLSDSFRYGAFQVVSILTTTGYVTSDYELWPFFIQYL
                     LLFLMFVGGCAGSTGGGIKNMRIIVLMKHVGAELKRLLHPKAIFTVRIGGRVLEREVI
                     SSVTAFFVMYIGLFSLAAMVMAALGLDVISAVASVAATIGNIGPGLGSVGPSENYAHI
                     PVAGKWVLSFCMLLGRLEIYTVMMLFLPGTWRR"
     gene            complement(8571..9935)
                     /gene="trkA"
                     /locus_tag="PK146_01570"
     CDS             complement(8571..9935)
                     /gene="trkA"
                     /locus_tag="PK146_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPE90957.1"
                     /translation="MKIVIIGAGEVGFSVASSLSQEGHDITIVESNDERAAKAENELD
                     VMVVRGNGSRPTVLEQAGICPGCDTDLLIACSNHDEVNIMACWIARRAGVKRVISRAR
                     GMEYTDSPTWARQLGIDVMISPERSVAREIEEFLSISSAVHTAEFFGGKVGVYAFRVS
                     SGSALMGIPLRDLRRIHPKITALVVFIERQGKGFVPSGEDRLEAEDLCFTVTSKSQAQ
                     DLEELFTKKKTPELRRVIIIGGGKIGYQVARRLEIRYPKLDIRLIDQDREKCEKLSRE
                     LERSIIIHGDGMDEELLRYEGVDEADGFVTTTANDEHNLLIGVVGKALGASKSVAVVR
                     RELLMRLTDYIAVDSMVNPNQALATVIMRQVRYPFGTGALSIIDEIDAEVLELSIPHG
                     STACGKRIMDLELPKGILIALVSRNGEMFVPWGDNDLKENDEVLIFASEELMPRAVEI
                     LGVE"
     gene            10028..>10356
                     /locus_tag="PK146_01575"
     CDS             10028..>10356
                     /locus_tag="PK146_01575"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HPE90958.1"
                     /translation="MPHREKIAFVGTGGTISMTFSSKQNGYVPTLSAQDLVEILPTDL
                     GRDLQVIDWSHQPSSHYTIRMTTDLVELLRKLVKDGASGIVVTCGTDSLEEMAYLTDL
                     LWAYPQPV"
BASE COUNT         2297 a         3170 c         2652 g         2237 t
ORIGIN      
        1 aggacaaagg tctccatacg tgacccggcg atgccctgtc atccctgtgt ccggtcgatc
       61 cttacggtac cggcccatga acaccccctg tcgaagccct atatccccaa cagccaaatc
      121 catatcggga gggagaagac cgagagggcc gtactcaggg ccacgatctt gccggcgtat
      181 tcgtggtcca tccccatttt gtgggctatc acgaaactgt tgactgcggc aggcattgcc
      241 gaagtcagaa caacagcgtt ccgcagcacg ggatcaacag gccaggccag gaaggcacac
      301 cagacaagcg ccgggtttac aaaaagtttg aagaggctgt ctctccatgc cgatttcatg
      361 gccccgcgga ggttttcgaa actgatcgag gctcccaggg cgatcagggc tatgccgctt
      421 ccggtgtctc ccaccatttt aaccgcggaa tcaagccaga aggggagcct gttgaaccct
      481 gcgaatgcac cagccatccc cataatgcta gccaggataa gggggttctt caatagctgc
      541 agaagtgtat cgtgaaggga tttcagggtc agtcttccgg aaaggatcac ctgtccccag
      601 gtaatggaga taaaattgaa ccccaccaaa gatacggcaa ggtataaaga aacggcctga
      661 aatccttcct gtcccatggc gatcgtaacc gcggggatcc ccataaaaac gttgttcgat
      721 ctaatcgagg ccagcaccga tacggcaagc ctcctcctgt cactggttga gatccttgca
      781 aggatcaggg ctatagccgg gacgatcaga aatgtcgaat ggaccgccca gaagaggttt
      841 ggttcagaga gtatgcctgg accgacccca aggatcgacc ggaacagaag ggccggaaga
      901 gcggcccagt acagtatcga attaagacgg tggacagctc tcccatccag gagaccgatg
      961 cggcggaaaa ggaccccgag tcccatgacc agggccagtg gcaggaccat caggaaagaa
     1021 tccaaaacaa cctccccata tcagaaaaag aaagggcacg aaagagccgt cgcgccttgg
     1081 tcagggcgcg acggctaaca gggccggcaa cagggtgtgc tagaagcggc gctccttgat
     1141 ccttgcagct tttccgctga gcttcctgag gtagtagagt tttgccctgc ggacccttcc
     1201 atggcgtacc acttcgatgc ggtcgacgct tggacagtgg agcgggaaga ccctctccac
     1261 accgaccccg cccgatactt tccggacggt aaaggtctct cgcatgcccc cgtgcttcct
     1321 ggcgataacg atcccttcaa agacctggat cctctccctg ttcccctcgg tcaccttcac
     1381 gtgtaccttg acggtgtcgc ccggtcggaa ctcgggagtc tcctttctca ggtatctgct
     1441 ctctataaga gctatccttg agtccattgt cattccctcc ctgttgaata ctgcctgtag
     1501 atgctaacgc cggccgaaaa atctgtccag gatgacggcc aatgtatccc ggggacagga
     1561 aagggttctg tcatccctac cgcccctgac cggacccagg gacacctgtt gccccggatc
     1621 gggcgtaaaa ctctcggctc cctcgcctgc caggaatact atgggtctcc ccgatgcgag
     1681 cccgagcctc tttgcttcaa gccaatggat agatcccctg tcagtacttc cgcagatgat
     1741 aacgaaggga tcttctttct cacggtcctt gatccaggca atggctcttc ccggtgatcg
     1801 aaggactttc cctttccctc caagactctc ctggagttct ttgtgaaagg cctcatcggt
     1861 actgatgaat atggccctct cgcaaccgaa ggaggcacca aggtccgcaa tggcctccgc
     1921 ttcttccagg gttacctccc gtctggctgg gacaagtatg acataaacgc ccttgtcaag
     1981 gtagggtcca atattcgccc ttgcgatcat atcgggcctt cttccgagtg tcctcgccac
     2041 ggtctgccgg cgcctccagg aacttgtctt taggtggtca cctccggtga ggactctggg
     2101 gacctcgctc cccttccacg aagaaggcct cgtgtaatga ggtgtatcaa gcatacctct
     2161 gaagaaggaa tcctcctcga cggcggcttc ctgccctacc acacccggaa caagacgggc
     2221 tacggcgtcg atgatcacca tggcaggcag ttctcccccc gtaaggacat aatcccctat
     2281 ggagatctcc aggtccacgt acttgtcaac gaaccgttcg tccactcctt cgtaccggcc
     2341 gcatacgata acgaggtgct ctttcgacgc aagggattcc acaagatgct gtcccaggac
     2401 cacgccctgg gggcccggat gcaccacaaa ggcctgacct ccggcttttg cttcatcaac
     2461 cgctctggca aggggttcag ccatcaacac catccccccg ccgccgtagg cgtaatcatc
     2521 gatctggccg taatgtccat cagaatggtc gcgagggtta ataaaacgga tgtcgataag
     2581 actcttttca acagccttcc ctattacgcc ggtcgagaag aacggaccca gcaactccgg
     2641 gaaaggggtg atcaccgtta cgatcatcga tcccagagcc cctccggcag acttatctca
     2701 ataaccccct cttcgggtga gactctaagg accacttcgc ggactgccgg gatcatcctt
     2761 acgtcgcctt caggtgtcct tagggagtag acatcatgaa ctcccgtccg gagaacatcc
     2821 tccaccttgc ccagctcttc gcctgttcct gagaggacta ccgcaagacc cacaatatcc
     2881 tcgatccagt aactgccctc aggcaagggg accctttcgg aaggatccac cataatgaga
     2941 ccgccctcaa gggcctgtgc cttatcccga tcgctgatat cttcggaccc tacaagaaag
     3001 aggcccttcc cctcgtgaag tctgatggtt cggatcgaaa gcgaagctat cagcttcccc
     3061 tccggccgga agatgtcgat cctgtccata tcgaggaaac gctccggaaa atccgtgagc
     3121 ggaaggacct tgatctcgcc cctgaccccg tgggcgccaa ggatccgtcc tatagtcacc
     3181 attgtgggtc ctttcaaggc tgccacctca gtcttccagg atgtccactt ccactttctc
     3241 gccgggtttg acagcggcag ctctggcaac catcctgatg gcgtttatcg taacgccgtg
     3301 cttgcctatc agccgcccca tgtcgtccct tgaaaccctt accagtacct ttacaactcc
     3361 tccgacgctt ctctgtgacg tgatcgtcac accgtcagga tcggaaacaa tgctcctgac
     3421 gatgaacccc acgagttcct cgtaatccgg catgagcgga tcccgttact gcttcttccc
     3481 ggatgtgaac ttgtcccaga ctcccgtctt cttcaggatc gcccgggccg tatccgacgg
     3541 aagcgctcca acacccagcc agtaaagggc cctgtcctcc tggaccttca gcgctgccgg
     3601 ctccttcatg gggtcgtatg accctatctg ttcgatgaaa gcaccgtcac ggggcgccct
     3661 cgagtcggca actaccagtc ggtaaaaggg ctgcttctta cggccgaacc ttgctaggcg
     3721 aatacgaact gccatatgtc tgccacctcc tgtatttatt ctactgttcc atagatccaa
     3781 taaatgccgt ttccatgaac aaggggcgcg gcggtccttc ctacatggga atggggggga
     3841 atcccctggg cagtttcgcc cctttacctg gagcgaaccg cttcatcaac atgctcatct
     3901 gctcgaactg cttcaggacc tgatttacca tctgcaccga ggtacctgac ccctgggcga
     3961 tccttctgcg cctgctgccc ttgattatct gggggttctc cctctctttt tttgtcatgg
     4021 acaggatcac tgcctccaca tgggcaagtc gcccggggtc gagctgaaca ttgtccagcc
     4081 ccttgaccct gcccgcaccg ggtatcatct cgagaacctt gtccaggggc cccatcttcc
     4141 tgacctgcct gatctgaagg agcatgtcat ccatggtcat cctcttcttt ttgaccgaag
     4201 ctgctatctt ctcgatctcc ctgtcgtcgg cagcctcctg gatcctctcc accagaccct
     4261 ggacatcccc catacccaga acgcgttggg ccatcctccg gggatcgaac tgctcgatgt
     4321 cagtgacctt ttctccaaca ccagcgagtt tgaccggaac cccggtggct gcccttatgc
     4381 tcagggcagc ccctcccctt gagtcaccat caagcttcgt caggacgatc ccgctaaggg
     4441 ggatcttgtc gttgaaggca gctgcgacgt tgacagcttc ctgacctgtc atcgaatcga
     4501 ccaccaggag agtctctgtc ggggagagga tccctccaag agaggcaagc tcctccatga
     4561 gctggtcatc gatgttgaga cggcctgccg tgtcgaagag aatgacatcg taaagcctcc
     4621 gggccgcgta ctcctctgcc ctgcggacaa gagggaggac atccttctct cccccagagg
     4681 ggccaaagaa gcccactccg gcctgcccgg cgagtatcct gagctggtcc acggcggcag
     4741 gtctcctcag gtcgcatccg accactagag gcttgtggcc gtcggagatc ttcttggcta
     4801 tcttgacgca ggtcgtcgtc ttccccgaac cctggagccc cacaaggagg tatttcgtcg
     4861 gaggtttcgg ggagaaggac aaggattgac gccccgagcc catcagagcc gccatctcct
     4921 cgtagactat ggcgatcacc tgctgggcag gagtgatcga gtcgagcaca tcccttccgg
     4981 cagcacgttc acggatcctg tccaccaggt ccttgacaac ccggaagttg acgtcaccct
     5041 ccaggagcgc cctcctgaca tccctcaagg cgacggagat gtcctcttcc gtcagtttcc
     5101 ccctgctcct gagccctttg aacacacctt caaggcgttc cctgagcgaa tcgaacataa
     5161 ggcgtatcag ccctcccgct cttcaggggc aagcaagacc gcaagttcgg cggcagcttc
     5221 ttgggatatc ttctccatat ggcgctggta ccatctccct agttccctga agtgcctttc
     5281 ccatttggca tcctttgcag aaaaacccag cagtccctcc atctccagga ggcggtccct
     5341 gcccctctga acgagatcat gggctccctg cctggtcacc tcgagtcttt cggcgacctc
     5401 ggagagcgac cagtcctcca gcacgtggag acgaaaggcc tcccgctgtt tctctgtaag
     5461 gaccggctcg taaaagtcga agagtcttga aagcattacc ctgtgctcta tctggtcttt
     5521 caatgggaca cctccaaata tgcacagtgg acgattatag gagtccgctt caggcctgtc
     5581 aagtataatt acttgacagc aaaacggggg gtgccatatc tggcatcccc cgggtttgtc
     5641 atgttttttc acgaaaatcg ttcaagcatt tttggaggtc gcagttttct cccgcgtttc
     5701 aagggtttcg gccctccctg tctcctgctg cgtctcgcat gtctcccggg ttgcttcacg
     5761 atgcacgatt tacgattcac gatctactag tcatgcgtct cccgttacga tccaggcctt
     5821 acccgatccc aacctaatgc tcatgcccat ggtggtgacc accaaaggcc ttctcctcgg
     5881 ctttctggag aaggtcccac aggacagcca ggtcttttct gcgggatcct atcaggtacc
     5941 ccagggagtg gcggatctct tcatcttcta tgatgtggta tcttgctgcc agttcggtca
     6001 tggcatcgag ctcctgcgac agggtctctc tcagaagctc cgcaatcgaa agcggatcat
     6061 cgacatgggc gtgtcctcca ctctgatcgg gggttatcac tctgcacatg gttatccctc
     6121 cattctcttt agcataagag aataacactt tttggcaaaa ttgtcatact gatccatagc
     6181 ctcgggcctt ttcatatccc gtcggtgttc tgataaaatt gggacgctgg aaccaaggga
     6241 aaaaacatta agggaaaggg gaaagaccta tgaatccgat gcagaagaag aaccttgaag
     6301 tcagaaggac ttataacgag cacctcggca agagcgtggt gaaggccctg gaggccaacg
     6361 ggttcaaggc ggtctatgcc gggtccagcg aagaggccgt taacaaggtg atggaactgg
     6421 tcccggaggg ctcttccgta ggggtccccg gatcatcaac catccgggaa ctggggctct
     6481 tcgaagagct ccaggccagg aattgcaggg ttttcgagca ctgggacccg tcgctcgccc
     6541 ctgaggacag atcgaggaga tgggaagagg agaacaccag cgatgtcttc ctttccagct
     6601 ccaacggggt cactctggac gggatgctgg tgaacatcga cggaacgggc aaccgcgtgg
     6661 cagccatggc ctgggcaagg aaccggatca tctttgtcgt gggcatcaac aaggtcagtc
     6721 gcgatataca gtccgctacc cagaggataa gggacgtagc cacaccgctt aacgccatca
     6781 gcaaagggat agacatccct tgcgcaaaca ccggcttctg tgtcaactgc aaggtcccga
     6841 accggttctg ccgggccacc cttgtccttg agagagcccc cttcggacgc gacgtacatg
     6901 taattatcgt cggggaggag ctggggtact aggatccgtc agaggttaga agtaagacga
     6961 aaaagcaggg cataaaggcg cgtaaacagt agacggtgaa aaactgttta cgcgcctttg
     7021 ttttttgcgg cttgcgattc tcggcccgtt tgctgtttac cgttcaaacc ctttattctt
     7081 gggtttacgg atcaggtctc acgcctcctg aatcttccta tctcctccag gtcccgggga
     7141 ggaaaagcat cataaccgta tagatctcga gcctcccgag aagcatgcag aaagagagga
     7201 cccactttcc cgccacaggg atatgggcat agttctccga gggacctact gaacccagcc
     7261 ccggacctat attgcctata gtcgccgcaa cactggcaac ggccgaaatt acatccagac
     7321 ccagggcggc catcaccatg gccgcaaggg agaaaagccc tatgtacatc acgaaaaaag
     7381 ccgttaccga ggatatgacc tccctctcca ggaccctgcc cccgatcctt acggtaaata
     7441 tagccttggg gtgaaggagc ctcttgagtt cggcaccaac gtgtttcatt aggactatta
     7501 tcctcatgtt ctttatcccg cctccggtcg atcctgcgca tcctccgaca aacatcagga
     7561 acaacagaag gtactggata aagaagggcc aaagttcgta atcagaggta acataacccg
     7621 ttgttgtcag aatgctgacg acctggaaag ccccgtaacg gaaagaatcg gacaaggttc
     7681 cgtaggtccc ggtagcgaaa aggaccgcag tggcggcaag aacagatgct actgtgatct
     7741 ttgcatagaa acggaactcc tcgtcacgcc accatgcgcc aagatcaccc ttgagagcga
     7801 gataatgaag cacgaaattg gcccccgcca ggaacatgaa aagggttatc acccattcga
     7861 agtacgggtt cccgtattgg cctatgcttc cgttgagcgg ggagaaccct ccggtcgcca
     7921 tcgtaccgaa tgtgtgggtc agcgattcaa agaggtccat ccccccaagc atcagcgccc
     7981 cgacctgaat cgcagaaagc agaacataga ctccccagag caaaagtgcc gtttggcgga
     8041 cccgtggagt gatcttctcc ggcacgggtc cgggcacctc agccttgtag agctgcatcc
     8101 cgcctacccc caggaagggc aggatcgcta ggctgagaac tatgatcccc atgcccccaa
     8161 gccaatgggt gagatcgcgc cagaagagta tgccccttgg attggactgg atatccgtca
     8221 aaatggaagc accggtagtt gtgaaacctg acatggcctc gaaaaaggca tcggtgaagc
     8281 tggagaccgt cccgttcaga aggtagggta ctccaccaat agccgaggca aagacccacg
     8341 agagagagac cactgcgaag gcctcgcgta cacccaggtc ccttgctttt gccttgaagc
     8401 cgacagcaaa aagaaccgcc gaaactaaca gcccggtagc catgccgcca gacaaggccg
     8461 gaaggtcgtt tgaaccgtcg gccagtgacc atatcatagg gaacaccatg aatagcgaga
     8521 cgagaagaca gagaagagag aggaccttgg caacgacggc cagtctcaaa ctattccacc
     8581 cccagtatct cgactgccct gggcatcagt tcttccgagg cgaagataag gacctcgtca
     8641 ttctccttga gatcgttatc cccccacggc acgaacatct ctccgttcct cgagacaagg
     8701 gcaatcagta tgccctttgg gagctccagg tccattatcc tcttcccgca ggctgtgctg
     8761 ccgtgaggga tcgaaagttc cagaacttcg gcgtcgatct cgtcaatgat cgaaagtgcg
     8821 cccgttccga aggggtagcg aacctgcctc attattaccg tcgccagggc ctggttaggg
     8881 ttgaccatcg aatcgacggc aatgtaatcc gtaagcctca tgagcagctc ccgtctcacc
     8941 accgctacgc ttttactggc cccaagggcc tttccgacca caccgataag aaggttgtgc
     9001 tcgtcgtttg ccgttgtagt tacaaatccg tcagcctcat ctaccccctc atacctgaga
     9061 agttcctcgt ccatgccgtc cccatgaatt attatcgacc tctcaagctc cctcgagagt
     9121 ttctcgcact tctcgcggtc ctggtcaatg agcctgatat cgagcttggg gtatcttatc
     9181 tcgagcctcc tggccacctg gtacccgatc ttccctcctc cgatgatgat aactcttctc
     9241 agctccgggg tcttcttctt cgtgaacagc tcctccaggt cctgggcctg ggactttgag
     9301 gtaaccgtga aacagagatc ctcagcctcg agcctgtcct ctcccgatgg cacgaaaccc
     9361 tttccctgcc tttcgataaa gacgaccagg gcggtgatct tcgggtggat acgacggaga
     9421 tccctcaggg gtatccccat gagggccgaa ccggagctga ccctgaacgc ataaaccccc
     9481 accttgcccc cgaagaactc tgcggtgtgg acagcagagc ttatggagag gaactcctct
     9541 atctccctgg caacggacct ctccggcgag atcatcacgt ctatccccaa ctgtctcgcc
     9601 caggtggggc tgtcggtata ctccatccct cgtgccctgg agatcactct cttcaccccg
     9661 gcacgacggg cgatccagca ggccattata ttgacctcgt catggttgga gcaggctatc
     9721 aggaggtccg tgtcacagcc gggacagatg cccgcctgtt caagcaccgt gggtctggaa
     9781 ccgttccccc ttacaaccat tacgtcgagc tcgttctcgg ccttggcggc cctctcgtcg
     9841 ttgctctcca caatggtgat atcgtgcccc tcctgggaaa ggctggaagc tacactgaac
     9901 cctacctctc cagctccgat tatcacgatc ttcatcttct gcttcacccc ccgggaggaa
     9961 taaggcaacc ctgactctgc ctgcaacaga aaagtggtaa acttatccgg acaagggagg
    10021 tggaccaatg ccgcaccgtg aaaagattgc ctttgttggg accggcggca ccatcagcat
    10081 gaccttcagc tctaaacaga acggctatgt ccctactctc tccgcccagg acctggtaga
    10141 gattctcccg accgacctgg gaagagatct ccaggtgatc gactggagcc accagcccag
    10201 cagtcactat accatacgta tgaccactga tctcgtagaa ttgctgcgca aactcgtcaa
    10261 ggatggtgct tcgggaatag tagtcacctg cgggaccgat tccctcgagg agatggccta
    10321 cctgaccgac ctcctgtggg cgtaccccca gccagt
//
LOCUS       DAOPKQ010000022        10299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_6819_length_10299_cov_4.561597, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000022 DAOPKQ010000000
VERSION     DAOPKQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6819_length_10299_cov_4.561597"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PK146_01580"
     CDS             <1..1085
                     /locus_tag="PK146_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPE90959.1"
                     /translation="PFYNELTRKERGGPRFYIADNADPEGTSAILDMIDPTRTAYVVA
                     SKSGSTAETMANFLVFLDSLAGSGVKSPGGNVLVVTDKEKGSLRSFAEETGCRSFQVP
                     STVGGRYSVLSPVGLLSAGAQGVDINSLLLGASAMKSSLSKKKGISGDPAMVMAASAI
                     HHFRKGRNIITFMPYSGNLDSFSEWFCQLWAESLGKNGWGSTPLKAIGATDQHSQLQL
                     FAEGPDDKLFLFIVPTSRRDLLMPAPSWSSLDDLSYLEGKGLGSMLDLEALSTAAALV
                     KKCRPVLWLEIPVLDAFHLGAMVFFFEYVTAVTGLALDADPFDQPGVEQGKRYTYGLM
                     GRSGFESDAAEAREHFSSILGISACL"
     gene            1107..1955
                     /gene="panC"
                     /locus_tag="PK146_01585"
     CDS             1107..1955
                     /gene="panC"
                     /locus_tag="PK146_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPE90960.1"
                     /translation="MPHVARKVSEARKYLEGKRSSDSRVGLVPTMGFLHEGHLSLVRR
                     ARRENDTCVVSIFVNPLQFGPTEDLEAYPRDMDRDLDLLSSEGVDLVFAPDPDEMYSP
                     DRSVTVTERDISRSLCGSARPGHFDGVCTVVLKLFNILSPDRAYFGEKDYQQLQVVRR
                     MARDLDVDVEIRGCPIVREKDGLAMSSRNIYLGEAERRDALALSKALVEARSAFEAGE
                     RDCRKILVTVRRVLEAHPLVNIDYAEIRDAETLLPVERVEGRAVVALAARVGKARLID
                     NTFLEP"
     gene            complement(1961..2689)
                     /locus_tag="PK146_01590"
     CDS             complement(1961..2689)
                     /locus_tag="PK146_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE90961.1"
                     /translation="MTPYPNVSGRIFSKIGEAIGRFGMIAQDDRITVGLSGGKDSVLL
                     LLGLSGLRKRSPVRFSLRASMVDITNGKCDTGPIEDLCGSLGIPFNRITSPVLEVIRE
                     REEKSPCSLCSKMRAGVLFTEALDNGSTTVAMGHNLDDAVETALLNLFYAGRFRSFHP
                     RSWRSRTGLWLIRPLVFLSSSEIADEVERLDIELVPPLCPFSKDSKRERIKELVRSLE
                     IEIPDVRSQILHALMEPNSGDIWS"
     gene            2896..3549
                     /locus_tag="PK146_01595"
     CDS             2896..3549
                     /locus_tag="PK146_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE90962.1"
                     /translation="MEKTRVLLVAPQRLVREAIGRAIDQEEGLSLAGATDDSLEAFRL
                     AREARPDVVVFDLDSGRMDWPHFVRELSLSCRGVRFLALSYDGSPERISALCEAGIHG
                     CVCLSSGFDELVRGLHSVGEGELYFDQEISSELPEVLSKVNEGVILLRELTAREREVV
                     CLLSQGFSNQQIAREMVLSEKTVKNHISHILRKLELKDRTQVAILAWKTGLAVNGPS"
     gene            3590..4645
                     /locus_tag="PK146_01600"
     CDS             3590..4645
                     /locus_tag="PK146_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HPE90963.1"
                     /translation="MSVRRGLAAFLALSMGVSAIVLLSSFDRSTFEMIVHAHKVRLVC
                     VLLLVILAWAFDALKLFFVVKAAGEGISCKLAVLLNWLRYFGCAITPMQSGGGPFQVY
                     FLFKSGIPIGKGVAITLTSTLMTLFQLGFIVPVALFFQPELLQGRKLLQGVFSYVLVF
                     VLVSWSIVVISLLRPRIIKKWAGGITLLLKRWGFLKPVKVLRVVRRINREIDTYNRNF
                     RIFFSSGMPLFVLGFLFSCLQMVAMFSILPCLIWSVGLEVRFIEAFLAQALFLFILYF
                     VPTPGASGVAEGGGAAIFSLLVPWNIAGVMAITWRFFTEYLSIAMGAIVAVRMLGWGM
                     AEELLKSGEEGTGENPV"
     gene            4642..5211
                     /locus_tag="PK146_01605"
     CDS             4642..5211
                     /locus_tag="PK146_01605"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPE90964.1"
                     /translation="MNLDRMGSLAFRFRGGIWTLFFLLILFLSRPGTAGPLYGLIPVA
                     LGQGIRFWAAGTIRQYRGEEVGAEGLVTWGPYSIARNPLYLGNALIGVGWCVLSGSFA
                     AFIIFSAVFVFLYLMLVIPYEESFLRKNFPVDFARYSERAGRFFPLKAPSLAELRGPF
                     DRVVLWRSERHSLWVTLAGTLLILSRRWW"
     gene            5211..5729
                     /locus_tag="PK146_01610"
     CDS             5211..5729
                     /locus_tag="PK146_01610"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPE90965.1"
                     /translation="MLAWHRRGINEEVSSSVLDSPCGMWLVSWVPGGLCGITSCRFSG
                     KGKVTQEVLPIWLERSWEFFWRGERPLVTYCYEHEIPEFSRRVLEVVEAIPFGEVLTY
                     GQVAERAGNPKAARAVGSVMRYNPWALFIPCHRVIGSDGSLRGYGGPRGVSLKKRLLA
                     YEKKVKAEISRR"
     gene            complement(5791..7035)
                     /locus_tag="PK146_01615"
     CDS             complement(5791..7035)
                     /locus_tag="PK146_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPE90966.1"
                     /translation="MPTDRTRALELHRKARGKIKIYPTINIHNEEDMALAYIPGSVPP
                     ALAIQEDEGMSFEYTGRGNRIAVISDGSAVLGLGNVGPCAALPVIEGKCLLFKLFGDV
                     NAIPVCLDTQDTEDIIHFCTLLAPTLGGINIEDIASPKTFTIVQELRSRINIPVLCDD
                     QHGTAVIVLAGIHNALEVQDRKLRDMKIVINGAGSAGIATAELLLKAGAENIVVLNSS
                     GILNEDNPRMNHVQKELAEKTNPEKRSGGLEKAIEGANIFIGLSKGGVFPAELIKLMS
                     GDPVILAMALPDPEILPEEAIAAGAAIVATGGPGFQNAMPNTLSSPGIMRGLLDVRAT
                     VLNHNMLLEAARALADVVDRRRLGPGKIMPDIFCDEAAPRVAEAVGQAAIAEGFATRE
                     VPKGEIYNNLWQSLYGEQIMRF"
     gene            complement(7070..7627)
                     /locus_tag="PK146_01620"
     CDS             complement(7070..7627)
                     /locus_tag="PK146_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPE90967.1"
                     /translation="MRPFRIDPEKVQLLVIDLQEKMLPAVHGWEKVDRNTGVLIEACK
                     VTAVPVKYTEHYPKGLGKTVPSVMEKLPVQAGRMEKIHFSCCMEEGFEDFLRSGGRDQ
                     VIVAGVESHICVLGTVIDLMAIGMRVVLAADACSSRDPEHHRLACDAMVSAGALVVPV
                     ETVVYQLLGRAGTEQFKSMLPFFKA"
     gene            7765..8424
                     /locus_tag="PK146_01625"
     CDS             7765..8424
                     /locus_tag="PK146_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90968.1"
                     /translation="MGANDPKRSNEWEGFDPSEEWTVGDILKAMSGRDCPSYIERLAS
                     QIQEWMVSALAGEGEISAEDFLFGFGDRKQGDPEALISQLEWVDKFADILPATTEPIG
                     HEDAVVLCLGPVDFDEGMRMAIDYAALFGRDHCQRVWMLSDSWVIGEVVRYVQHFSAL
                     ASHGVELRFIMVTPWGWTEIPIAVETGDNGRLRWENRTARQEHNNNPLRPNKHDDPNQ
                     K"
     gene            complement(8494..8691)
                     /gene="thiS"
                     /locus_tag="PK146_01630"
     CDS             complement(8494..8691)
                     /gene="thiS"
                     /locus_tag="PK146_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPE90969.1"
                     /translation="MIVVNGEPMEWKEGMTVRDVINEKRYVFPLLAVWIDNSSVPRDH
                     FATTPVPDGANVQIIHMISGG"
     gene            complement(8696..>10299)
                     /locus_tag="PK146_01635"
     CDS             complement(8696..>10299)
                     /locus_tag="PK146_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPE90970.1"
                     /translation="AAENTFWGCLNSSFWDSHRKTARLKQAGRGGGGTILRDKKIAAL
                     VVKTHDLSGTANNPADVDTLTRLGAKLHKEIITLDAKQCRMRTAGTSHLNEIMNEYHL
                     LPTMNYKFGQHPDADKIHSKIYEKMFTQGISDGCWYGCTMACAKAVDGFELKTGPWKG
                     RKITVDGPEYETAASFGPNVGVYDPAWTTETNFYADHYGFDTISAGTGIAFVCECYEL
                     GLINKENTNGLELNFGNKDDIMELIHRMASGKDEFARMVGQGVHRMKDLFAEKFGADR
                     NVMERIGMEGQGIEVSEYVPKESVAQWGGFFLTLKGPQHDEAWLIFMDRVNKQLPTFE
                     DKAEALHYFPNFRTWFSLVGLCKLPWNDIEPADNATKYPPQEAARVPEHVQNYVDIFN
                     AVTGQNITKEDLIHQAERVYNFQRVFQLRLGKGTRKFHNIPDRALGPVWADEWEARAE
                     YYDNELKAGGVDPAGLSSQEKCDRLQKIRREQWEKLVDTVYRRRGWDKNGIPTVKKLK
                     ELGMDIPEVVAVVEKHAKPEDQWKD"
BASE COUNT         2037 a         2834 c         2921 g         2507 t
ORIGIN      
        1 accccttcta caacgaactg accaggaaag agagaggggg tccgcgcttc tacatagcag
       61 acaacgccga tcccgaaggg acgtcggcca tcctggacat gatagatccg accaggacag
      121 cttatgtcgt tgccagcaaa tccggatcaa cagccgagac aatggccaat tttcttgtct
      181 tcctggactc cctggccgga tcgggggtca aaagccccgg agggaacgta cttgtcgtga
      241 ccgataagga aaaggggtca cttcgttcct ttgcagaaga gacggggtgt cggtcattcc
      301 aggtcccctc cacggtcgga ggaaggtatt ctgtgctctc acccgtggga cttctctcag
      361 ccggtgctca gggcgtcgat atcaattccc ttctcctggg agcctcggcc atgaagtcaa
      421 gcctttccaa aaagaagggg atctccgggg atcctgcaat ggtgatggcc gcttcggcga
      481 tccaccactt ccggaaaggg aggaatatta tcactttcat gccctattcc ggcaatctgg
      541 attcattctc ggagtggttc tgccagctct gggcggagag cctgggcaag aacggttggg
      601 gaagcacccc cctgaaggct attggggcga ccgatcagca ctcacagctg caacttttcg
      661 ccgaaggacc tgacgacaag ctcttcctgt tcattgtgcc cacatccagg cgggacctgc
      721 tcatgccggc tccttcatgg tcgagcctgg atgacctctc ctacctcgaa gggaagggtt
      781 tgggctccat gcttgacctg gaggccctct caactgctgc cgcgctggta aagaagtgcc
      841 gtcctgttct gtggctggag ataccggtgt tggacgcctt ccacctgggg gcgatggtgt
      901 tctttttcga atacgtcacc gccgtcaccg gtcttgccct ggatgccgac cccttcgatc
      961 agcccggagt ggaacagggc aagaggtaca cctatggtct tatgggaaga tccggctttg
     1021 aaagcgatgc cgccgaggcc agggagcact tctcatcgat cctgggcatt tcggcctgtc
     1081 tttgaagggt ggatcgttca tcaatgatgc cacatgttgc aagaaaagtt tccgaagcca
     1141 gaaagtacct tgaagggaag agatcttcag acagccgtgt tggtctggtc ccgacgatgg
     1201 gttttctcca tgaaggtcat ctttcccttg tcagacgagc gagaagagag aacgatacct
     1261 gcgtggtctc catcttcgtg aaccccctgc agttcggccc cactgaggat ctcgaagcct
     1321 atccaaggga catggacagg gacctcgacc tgctctcctc cgagggcgtg gaccttgttt
     1381 ttgctccaga ccctgatgag atgtactccc ccgaccgttc cgtgactgta acagagagag
     1441 atatcagccg ctctctctgc ggttcggccc gacccggcca ctttgacggg gtctgtacgg
     1501 tggtgctgaa gctattcaat atcctatccc ctgacagggc ctacttcggt gaaaaggact
     1561 accagcagct gcaggtggtc aggcggatgg caagggacct ggacgtcgac gtggagatca
     1621 ggggctgtcc cattgtccgg gaaaaggacg ggcttgcgat gagcagccgg aatatctacc
     1681 ttggagaggc tgaaagaagg gatgccctgg ctctttcgaa ggctctggtt gaggccaggt
     1741 cggcctttga ggcaggggag agggattgcc ggaagatcct ggtaactgtc agaagggtcc
     1801 tggaggcaca ccctctggtc aacattgact acgcggagat acgggacgcc gagaccctct
     1861 tgccagtgga gagggtggaa ggaagagccg tcgttgccct cgctgcgaga gtggggaaag
     1921 cgaggctgat cgacaatact ttccttgaac cttagccaga tcagctccat atatccccgg
     1981 aattaggctc catcagggca tggagtatct gcgaccgcac atcgggtatc tctatttcaa
     2041 gagacctgac aagttccttg atccgttcac gcttgctgtc tttggaaaag ggacataaag
     2101 gcgggaccag ttcaatatcc agtcgttcga cctcgtccgc tatctctgaa ctggagagga
     2161 aaaccagggg tctgataagc cagagcccgg tcctgcttct ccacgatctg ggatgaaagg
     2221 atctgaacct tccggcatag aagaggttga ggagagcggt ctctacggcg tcatccagat
     2281 tgtgccccat ggcgaccgtt gtggatccgt tatcgagagc ctcggtgaag agaaccccgg
     2341 ctctcatctt cgagcaaagg ctgcaggggg acttctcctc tcgttccctg attacctcga
     2401 gaacgggaga ggttatcctg ttgaagggaa ttccaaggga tccgcagaga tcctctatcg
     2461 gccctgtgtc gcactttcca ttggtgatgt ccaccatgga cgcccgcaaa gagaacctga
     2521 cggggcttct cttcctcagg ccggaaagtc cgagaagaag caggacgctg tccttgcctc
     2581 ccgaaagccc gacggttatc ctgtcgtcct gggcgatcat tccgaaacgg ccaatggctt
     2641 ccccgatctt tgagaagatc cggccggaga cattcgggta cggggtcatc ttaaccttca
     2701 gcacctcccg ctggagatgg atttgccgca agcacaggag ccgatgatag gttataatgc
     2761 cctcgaatag aggaccggct cagccttccc tgatctttta ttctgccatt ttacaccccc
     2821 ctgggaagga aaaggggctg tcgtggtgct gcgcaaggtc catcccgcca aggcttctcc
     2881 caggaggtgg tttttttgga aaagacacgg gttcttctgg tagctccgca aaggttggtc
     2941 cgtgaagcca tcggaagggc gatcgaccag gaggagggcc tctcccttgc aggtgccact
     3001 gatgacagcc tggaggcctt tcgtctggcc agggaggccc gtccggatgt tgttgtcttc
     3061 gatctggatt caggcaggat ggattggccc catttcgtca gggagctctc cctctcatgc
     3121 agaggggttc gcttccttgc cctctcttat gacgggagtc ccgagcgcat ctccgcactt
     3181 tgcgaagcgg gcattcacgg ctgcgtctgt ctatcgtcag ggttcgatga actggtaagg
     3241 ggtcttcaca gtgttggcga aggcgaactc tacttcgatc aggagatatc ctccgaactt
     3301 cccgaagtcc tgagcaaggt caacgaaggg gtgatcctgc tcagggagct aactgccagg
     3361 gagagggagg tggtctgtct cctttcccag ggtttcagca accagcagat cgcgcgtgag
     3421 atggtcctgt cggagaagac cgtaaagaac catatcagcc atattctcag gaaactcgag
     3481 ctcaaggaca ggacccaggt agccattctc gcatggaaga cagggctggc agtcaacggt
     3541 ccatcatgag aggaaatggc ttgaagggtc ttgcacggga ggagtaagtt tgtcggttag
     3601 aaggggtctg gcggcgttct tggccctttc catgggcgtg agcgccatag tgcttctctc
     3661 cagcttcgac aggtcgacct ttgagatgat agttcatgct cacaaggtcc gtctggtctg
     3721 cgtcctgtta cttgtgatcc ttgcctgggc ctttgatgcc ctcaaacttt tttttgtggt
     3781 caaggcggca ggtgagggga tcagctgtaa actggcggtc ctgctcaact ggctacgcta
     3841 tttcggctgt gccataaccc ccatgcagag cgggggaggt cctttccagg tctacttcct
     3901 gttcaagagc ggtatcccca ttggcaaggg agtagccata accctcacca gtacccttat
     3961 gaccctcttc cagctgggtt ttattgttcc cgtagctctc ttttttcagc ctgaactcct
     4021 tcaggggaga aaattgcttc agggcgtctt ctcctacgtc ctcgtctttg ttctggtctc
     4081 atggtcaata gtagtgataa gccttctgag gccccggatc atcaagaagt gggccggcgg
     4141 cattacactg ctgctcaagc gatggggttt tctgaagcct gtcaaggtcc tcagggtcgt
     4201 aaggcggatc aaccgggaga tcgacaccta taacaggaac ttccgcatct tcttctcgtc
     4261 gggcatgcct ctctttgtcc tcgggttcct gttctcctgc ctccagatgg ttgccatgtt
     4321 ctccattctc ccctgcctga tatggtccgt aggtctggaa gtcaggttca tagaggcctt
     4381 ccttgcccag gctctctttc ttttcattct ttacttcgtg ccgacccccg gagcgagcgg
     4441 ggtagcggag ggcgggggcg ccgctatctt cagccttctc gttccctgga acattgcagg
     4501 tgtcatggcg atcacctggc gtttcttcac ggagtatctc tccattgcga tgggggccat
     4561 cgtagcggtc aggatgcttg gttgggggat ggccgaagaa cttctgaagt ccggtgagga
     4621 agggacggga gagaatccgg tatgaacctg gatagaatgg gctccctcgc attccgtttc
     4681 agaggcggta tctggactct cttcttcctg ttgatcctct tcctctcccg ccctggaacg
     4741 gcaggtcctc tctatggact gatccccgtc gccctgggcc agggtatccg gttctgggcg
     4801 gcgggcacca tcaggcaata ccggggtgag gaggtcggcg cggaaggtct tgtcacctgg
     4861 gggccatact ccattgccag gaaccccctc tacctgggca acgcccttat cggggtcggt
     4921 tggtgcgtac tttcaggaag ttttgcggcc tttatcattt tctcggcagt ttttgtattt
     4981 ctgtacctga tgctggtcat accctacgag gaatctttcc tcaggaagaa cttccctgtg
     5041 gattttgcca ggtactccga aagggcagga aggttcttcc ctctgaaagc cccttccctg
     5101 gcagaactac gaggaccgtt tgacagggtt gtcctctgga gaagcgagag gcactctctc
     5161 tgggtcaccc tggcaggaac cttgcttatc ctttcccgga ggtggtggtg atgctcgcct
     5221 ggcacagaag agggatcaac gaagaggtat catcatctgt gcttgattct ccctgcggca
     5281 tgtggttggt tagttgggta cccggaggac tttgcggcat tacatcctgt aggttttccg
     5341 gcaaggggaa ggtgacgcag gaggtactgc ctatctggct ggaaagatcc tgggagttct
     5401 tctggagggg cgaaagacct cttgtgacct actgttatga acatgaaatc cccgaattct
     5461 ccaggagggt cttggaagtg gtcgaagcca tccccttcgg ggaggtgctt acctatggac
     5521 aggtggcgga aagggcaggc aaccccaagg cagcaagggc ggtcggatcg gtaatgcgtt
     5581 acaacccatg ggctctcttc attccctgtc acagggtcat cgggagtgac ggcagccttc
     5641 gcggatacgg aggtccgagg ggggtctcac ttaagaaaag gctcctggcc tatgaaaaaa
     5701 aggtaaaggc ggagatcagc agaagatgac ccccgcctca tttcccctgg tccgtggatc
     5761 ctcaattgac gatccgtcgt accagtgtcc tcagaatctc atgatctgct cgccgtagag
     5821 gctctgccag aggttgttgt atatctcgcc tttggggacc tcccttgtcg caaagccttc
     5881 agctatggcg gcctgaccta ccgcctctgc cactctgggc gcagcctcgt cgcagaagat
     5941 atccggcatg atcttgccgg gccccagcct gcgcctgtcc acaacgtcgg cgagcgctct
     6001 ggccgcttcc agcagcatgt tatggttgag caccgttgcc ctgacatcga gaaggcccct
     6061 cattatcccg ggagaggaga gggtgttggg catggcgttc tgaaacccgg gacctccggt
     6121 ggctactatt gctgcacctg ccgcgatcgc ctcttcgggt agtatctcgg gatccggaag
     6181 ggccatggca agtattaccg ggtccccgga cattagttta attaactccg ccgggaatac
     6241 ccctcccttg gaaaggccga tgaaaatgtt ggccccttcg atggctttct caagtcctcc
     6301 ggacctcttc tcgggatttg tcttctccgc aagctctttc tgcacatggt tcatcctggg
     6361 gttgtcctcg ttgagtatcc ccgagctgtt cagtaccaca atattctccg caccggcctt
     6421 gagaaggagt tcagcagtgg ctatccccgc tgatcctgcg ccgttgatca cgatcttcat
     6481 gtcccggagc ttccggtcct ggacctccag cgcattatgg atgcccgcaa ggactatgac
     6541 agcagttccg tgctgatcgt cgcagagtac cggtatattg atcctggatc tcagctcctg
     6601 gacgatggtg aatgtcttgg ggcttgctat gtcctcgatg ttgatccccc caagggtagg
     6661 ggcgagaagg gtgcagaaat gtatgatgtc ctccgtatcc tgggtgtcga ggcataccgg
     6721 gatcgcgttc acgtcgccga agagcttgaa gaggaggcat ttcccctcta tgaccggaag
     6781 agccgcacag ggtcctacgt tcccaagccc aaggaccgcg ctcccgtcgg agatgaccgc
     6841 gatcctgttg cccctcccgg tgtactcaaa gctcatcccc tcatcttcct gaatggcgag
     6901 agctggcggg acgctcccag ggatataggc cagggccata tcctcctcgt tgtggatgtt
     6961 gatggtgggg tagatcttga tcttccccct tgccttcctg tggagttcca gggctcttgt
     7021 cctgtctgtc ggcataggtt ttcctccctg ttataagtat cccgggtctt caggccttga
     7081 aaaacgggag catcgacttg aactgctccg ttcctgccct tccaagcaac tggtacacta
     7141 ccgtttcaac tggtaccacg agagccccgg ctgagaccat cgcatcacag gcaaggcggt
     7201 gatgttccgg atccctgctg gaacatgcat cggcagccag cactacacgc atgccaatag
     7261 ccataaggtc gatcacagtc ccaaggacac agatatggct ctccaccccg gcaacaatga
     7321 cctgatccct tcctccagac ctgaggaaat cctcaaatcc ttcctccatg cagcaggaaa
     7381 agtggatctt ctccattctt ccggcctgaa caggaagttt ttccataact gaaggaactg
     7441 ttttcccaag ccctttaggg tagtgctcag tgtactttac gggaactgcc gttactttgc
     7501 aggcttcgat caataccccg gtgttcctgt ccactttttc ccaaccatga actgcaggaa
     7561 gcatcttttc ctgaaggtcg ataacgagca gctgcacctt ttcgggatcg atcctgaaag
     7621 gtcgcatcaa aacacacccc gtttccttca ttattctcga ttatagggtt attataggcc
     7681 aaagacggcc tctgttgccc cgtgtctatt tcattgaaat atgctatgct ctgaaaactt
     7741 gaaagcccgg gggaggtgtg tttcatgggc gccaatgatc caaagaggag caacgaatgg
     7801 gaaggtttcg atccgagcga ggaatggacg gtgggtgaca tcctgaaagc aatgtctgga
     7861 agggactgcc cctcttatat tgagagacta gcttcccaga tacaggagtg gatggtgtcg
     7921 gctcttgccg gcgaaggaga gatctccgcc gaggacttcc tgttcggttt cggggacagg
     7981 aaacaggggg accctgaagc actgatctcc cagcttgaat gggtggataa gttcgccgat
     8041 attcttcctg ccacaacaga gcctatcggg cacgaggatg ccgtagtgct ctgtctcggt
     8101 cctgtggatt tcgacgaggg gatgcggatg gcaatagatt atgccgccct tttcggcagg
     8161 gatcattgcc agcgggtatg gatgctgagc gacagctggg tgatagggga agtcgtgcgt
     8221 tatgttcagc acttcagcgc cctggcctct cacggagtgg agctcaggtt catcatggtg
     8281 acaccctggg gctggacaga gatcccaatt gccgtcgaga ctggtgataa tggaaggcta
     8341 cgctgggaga acagaacggc aaggcaggaa cataacaata accccctgag gcctaacaaa
     8401 cacgacgatc ccaatcagaa atagccgacg ggacctcggc aaatggaaag ggggcaggcc
     8461 ttctggcctg cccccttttt gcatcgaact gttctaacca ccgctgatca tatggatgat
     8521 ctgaacattg gcgccgtcgg ggaccggagt ggtagcgaaa tgatccctgg ggacagaaga
     8581 attatcgatc cagacagcga gcagcgggaa gacgtatctc ttctcgttga taacgtccct
     8641 tacggtcatt ccttccttcc attccatggg ttccccgttc accacgatca tggagctaat
     8701 ccttccactg gtcctccggt tttgcgtgtt tttctacgac tgcgacaact tcgggtatgt
     8761 ccatcccgag ttccttaagt ttcttgacag tggggatacc gttcttgtcc cagccacggc
     8821 gtctgtagac ggtgtctacc agtttctccc actgttctct gcggatcttc tgcagtctgt
     8881 cgcacttctc ctgggaggag aggcctgccg ggtcgacccc gcctgctttc agttcgttgt
     8941 cgtagtactc cgcccgtgcc tcccactcat ctgcccagac cggcccgagg gccctgtcgg
     9001 gtatgttgtg gaacttcctg gtgcccttcc caaggcgcag ctggaagacg cgttggaagt
     9061 tatagacccg ctcggcctgg tgtatcaggt cttccttggt gatattctgc cctgtaacag
     9121 cgttgaatat gtccacgtag ttctggacgt gctccgggac cctcgcggct tcctggggcg
     9181 gatactttgt cgcattgtcg gcaggttcga tatcgttcca ggggagtttg cagagtccga
     9241 ccagggagaa ccaggtcctg aagttgggga agtagtgcaa agcctcggcc ttgtcctcaa
     9301 aggtggggag ctgcttgttg accctgtcca tgaagatcag ccatgcctcg tcatgctggg
     9361 ggcccttcag tgtaaggaag aagccgcccc actgggcgac cgactctttg ggcacgtact
     9421 cggatacctc gataccctgc ccttccattc ctatcctctc catgacattt ctgtcggcac
     9481 caaatttttc agcgaagagg tccttcatcc ggtggactcc ctggcccacc atcctggcga
     9541 actcgtcctt gcctgatgcc attcggtgga tcagttccat aatgtcatcc ttgttgccga
     9601 aattcagttc caggccgttg gtgttctcct tgttgataag gcccagttcg tagcattcgc
     9661 agacgaaggc tatgccggtc cccgcggaga tcgtgtcgaa tccgtaatgg tcggcgtaga
     9721 agttggtctc ggtagtccat gccgggtcat aaactcctac attggggccg aaacttgcgg
     9781 ctgtctcgta ttccggaccg tctacggtga tctttctgcc cttccagggg ccggtcttga
     9841 gttcaaagcc gtccacagcc tttgcgcagg ccattgtgca gccgtaccag catccgtcgg
     9901 agatgccctg ggtgaacatc ttttcataga tcttcgagtg gatcttgtcg gcatcgggat
     9961 gttgtccgaa cttgtagttc atcgtgggaa gaaggtggta ctcgttcatg atctcgttca
    10021 ggtgggatgt tcccgcagtt ctcattctgc attgtttggc gtcgagagtg attatctcct
    10081 tgtggagttt ggctcccagc ctggtgaggg tatcgacatc tgccggattg ttcgccgttc
    10141 ccgaaaggtc atgggtcttg accaccagtg ccgctatctt cttgtctctg agaatagtcc
    10201 ccccgccgcc tcgtcctgct tgtttcagtc tcgccgtctt tctgtggctg tcccagaagc
    10261 tgctgttgag gcaaccccag aatgtgttct ccgcggcct
//
LOCUS       DAOPKQ010000023        10239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_6873_length_10239_cov_4.339847, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000023 DAOPKQ010000000
VERSION     DAOPKQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6873_length_10239_cov_4.339847"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK146_01640"
     CDS             complement(<1..515)
                     /locus_tag="PK146_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90971.1"
                     /translation="MNRAGSFVVLNCALAILVALFSLSVIKGGAAEVLEGPLTISTSY
                     KSLLSTPDQKGMLDLLVKEAFSRIGLETEIVFNPTGRSLSDVNEGLADGELNRIEGME
                     KNFPNLVRVPEPNMVMDFVAFSKKEYIINGWESISDLRIGIVKGWKILEENTAGFPDV
                     TLVPTETELFN"
     gene            692..817
                     /locus_tag="PK146_01645"
     CDS             692..817
                     /locus_tag="PK146_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90972.1"
                     /translation="MWAQGELPDTPCCYNSAVADAVRDHLLLLIRVCTHIQVSTA"
     gene            complement(820..945)
                     /locus_tag="PK146_01650"
     CDS             complement(820..945)
                     /locus_tag="PK146_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90973.1"
                     /translation="MKAKAEDAISAVRRSILDDRVPPLLKEKTVVPLFFGDARSR"
     gene            complement(1579..2571)
                     /locus_tag="PK146_01655"
     CDS             complement(1579..2571)
                     /locus_tag="PK146_01655"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPE90974.1"
                     /translation="MKYIENRKTDPRFNLALEEVLFQSLDEKEEGFFLLWQNEPTVVV
                     GRFQNTLEEINVPVVREKGIHVVRRLSGGGAVYHDLGNLNFTFILRTENPQNLDFRKL
                     TEPVISALWSLGVDGGLVGRNDLVIQGKKFSGNAQYMREGRLLHHGTILYDSDMDALE
                     EVLQADPDKFKSKGVKSARSRVTNIRSHLKGEPDIEVFRRALLFTVKERWPGLEISPP
                     DPSVQEKAAALAESKYGTWDWNYGASPEFDLRKERRFGFGKVDVRLNVRKGRITSCRI
                     FGDFFGNGDIAGLENGLAGTQFSEKGLSGMLKRVDVSHYISGMDSRSFMELVLE"
     gene            complement(2604..4385)
                     /gene="lpdA"
                     /locus_tag="PK146_01660"
     CDS             complement(2604..4385)
                     /gene="lpdA"
                     /locus_tag="PK146_01660"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPE90975.1"
                     /translation="MERELMATMITMPKLGLTMTEGSVLEWKKNEGDTLKKGEVVLVV
                     ATEKLTYEVEAPDDGLLLRIAVKAGETVPVGAILGAIGEKGEEPDLGVAVAEEVPTSK
                     DEAASEREQERPTGSLKGTEPAPAKGDTGKRVVVIGGGPGGYVAAIRAAQLGGNVTLV
                     EKERLGGTCVNVGCIPTKALLHSAEVNRTVEDAAEAGLKVGSLEVQWDVVQERKDQVV
                     KQLSQGIKALLSAHGVEIITGEASFMDDRNILVRSGEGDRKIESDAVIVATGSEASLP
                     PISGVELGGVVTSTGALSFDKIPSRMVIIGGGVIGVEFASIYADFGCSVTIIEMLPQI
                     LPGADGEAVSALRKVLEARGITIHTGAKVGSIEAAGSSLVISADLSGREEKIGADKVL
                     VAVGRKPFTEGLNLEAAGIRIERDRISVDKSMRTSVPHIFAVGDCCSPIMLAHVAMME
                     GEVAAGNIFGLDEKMDYSTTPGCVYTSPELAWAGLTEEEAKKRGHKVRKGVFPMIGNA
                     KSLISGETRGMVKIVADEKYGEILGVHIVGARATDLISEASLAIRLEATLDEVAETIH
                     GHPTFCEATREAVLAGLERAIHSIPRV"
     gene            complement(4370..5872)
                     /locus_tag="PK146_01665"
     CDS             complement(4370..5872)
                     /locus_tag="PK146_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPE90976.1"
                     /translation="MEQVTMPKLGLTMTEGTVSKWKVSPGDRIKKEEVLLVVATDKLT
                     YDVTSPIDGVVLEILVKEGESVPVGKVLAVLGEAGEKVSAADRQGERPEPGPEEAVER
                     PSTSTPLSRGVGTNEDVPGPESKGQTAAASPLARKWARSFGLDIGAIEGSGPGGRVVK
                     DDVMKASTRSRATPLARKIAREAGISVQEISGTGPMGRTTAGDVKAYLASPPKERIRV
                     SPVAARLAEDLEVPLGTIKVSGRIMKGDVLEAACAAKESKTVNRRVPLTPMRKVIAER
                     MTFSASTIPSVFFNIETDFTEFIGFRKALNEELEGSGVKVSFNDILMKICARALVEMP
                     MANASYDPGRNEYILHDDINIGIAVAVEGGLLVPNVKKVQEKPLPVIAMESLELVNKA
                     RKSRLLPEEMQGGTFTITNLGMFGIHDFIPIINPPEACILAVNVVTERPVCRDGQVVV
                     RPMSMIGLTADHRILDGADAARFLARVRELVEKPWMTLVQEWQTDKWSVN"
     gene            complement(5907..6878)
                     /locus_tag="PK146_01670"
     CDS             complement(5907..6878)
                     /locus_tag="PK146_01670"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPE90977.1"
                     /translation="MREITFSQATLEAMDEEMTRDERVFVMGEDIARQGGIFGQFKGL
                     PDKFGKDRVKDTPISETAIVGAAIGAALTGMRPVADMHFADFMGVCMDEIFNQMAKIH
                     YMFGGQRSVPMVLRAPDGLINQAAAQHSQSIEAWFMHIPGLKVVIPSNPADAKGLLKS
                     AIRDDSPVIYFEHKALFNTRGAVPDGEVLSPIGKAKVAREGSDVTLVSYSHCMGLSLK
                     AADILAGEDVSVECIDLRTISPIDKETILASVAKTTRLVIAHEAVKQGGVGAEISAIV
                     AEEGIDFLDAPIVRVGAPFTPVPFARPLEQAYRVTPEKIAEAIRGLF"
     gene            complement(6900..7898)
                     /locus_tag="PK146_01675"
     CDS             complement(6900..7898)
                     /locus_tag="PK146_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HPE90978.1"
                     /translation="MSAKKVKEKKGLNKYPRTLLEQFYRTMLTIRSFEKKVEEKFLAG
                     EIPGFVHLYIGEEAIAAGVISNLSREDYIQSTHRGHGHTIAKGADLKRMMAEIFGKKE
                     GYCKGKGGSMHIADLSIGMLGANGVVGGGFNIATGAALATKMQGKNNVSVVFFGDGAS
                     NRGTFHEAANMAAVWDLPVLFVCENNQYASTTPYMTTTSVENIADRAAGYGMPGVIVD
                     GNDIFAVYEESQKLIDRARRGEGPSLLECKTYRVKGHYVGDPERYRTKEEVNKVCESN
                     DPIDRFECTVLDGKLFSREELDAIKASAGKEVEKALEFARNAEEPEISELFAGVYA"
     gene            complement(7975..9261)
                     /locus_tag="PK146_01680"
     CDS             complement(7975..9261)
                     /locus_tag="PK146_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPE90979.1"
                     /translation="MSIDPGVFLLGLFFIFLAFKVPIAVALGMAGMAVVYWYNLGAQM
                     FAPVFFANISKFSLLAIPFFILAGVIMGRAKISDKIIRLIMLMVGPVSGGMAIVTIIT
                     ALFWGAVSGSGPATVAALGTILIPGMVKAGYDRGFAAALTSSASGLAIVIPPSIGFIV
                     YGVITGASISELFLAGIIPGILVGLFLIVAAFIISVARGYKGDRFGTAKEVLTAFKDA
                     FWALLTPVIILGGIYGGIFTPTEAAAVGVAYGLFVGFVVYRTLTLKDLYELLVDACAS
                     SAVVMLVVAFAGIFGWAMMVLGVVERVSEGVIAMSSNPFVVIALINVILIISGMLLDA
                     ISIYYLLMPIFMPVIAHFGWDPVWFGVVMTINLAIGQVTPPVAVNLYVGCNVAGVSMD
                     EISRAVVPFLLASIAALAVIVYLPGITLWLPNLLMR"
     gene            complement(9258..9761)
                     /locus_tag="PK146_01685"
     CDS             complement(9258..9761)
                     /locus_tag="PK146_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPE90980.1"
                     /translation="MFKLLDRIEEWTCVTMLFFMAMLAFVNVIVRYLTVFSFSFSEEL
                     LVNLFVWITMLGASIATRKGSLIGVSYLFDRMPPSLKKLSTAVFVLCGVTLFGLLTMH
                     GIDMVKSEFRSGMTTYSMALPMWIFGMAVPVCAFILILRTIQRGVHEWNDLSGPQTGS
                     CPEVDQR"
     gene            complement(9864..>10239)
                     /locus_tag="PK146_01690"
     CDS             complement(9864..>10239)
                     /locus_tag="PK146_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE90981.1"
                     /translation="PIVGIIIPNKIYEVQKHLTNWHYSYDALILAVNKNVWESFDPDI
                     KEQIKKAAVDAMHWQVHDVSRVGLGDGIEFLKSQGMTVTDPTPEQLAAFREMTKAVYE
                     KWSGNVGADIVKAFEEAVSSFK"
BASE COUNT         2159 a         3113 c         2602 g         2365 t
ORIGIN      
        1 atattgaaga gctccgtctc ggtgggcacc agggttacat cgggaaagcc ggcggtgttc
       61 tcctccagga tcttccaacc ctttactatc cctatcctca ggtcgctgat gctttcccac
      121 ccattgataa tgtactcttt cttcgaaaaa gccacgaaat ccatcaccat gttgggctcg
      181 ggaaccctta caagattggg aaagttcttc tccatcccct ctatcctgtt caactctccg
      241 tcggcaagac cttcgttgac atccgagagg gaccttcctg tagggttgaa gactatctcg
      301 gtctcaagac cgatcctgct gaatgcctcc tttaccagga ggtcaagcat tcctttctga
      361 tcaggggtcg aaagaaggct cttgtaggag gtgcttatcg tcaatggacc ttccaaaacc
      421 tctgcggcgc ctcctttgat gaccgaaaga gagaataacg ccaccagtat tgccagggcg
      481 caattcaaaa cgacgaagga cccggctctg ttcaaagtgc atcacccttt ctttcgacat
      541 aggaggtgac ggggacagtt gcgatgtctt tgtttcccaa aaccaggaac aaggcgatta
      601 tatacctctc gtctttgaaa tgaagacaga tcttcactgg attccttcta agttttattt
      661 tattactatt atacctgtat tgtcacttcc cttgtgggct caaggggaac tccccgatac
      721 tccatgctgt tataattcag ccgttgccga cgcagtgagg gatcaccttc tgctgctgat
      781 cagggtctgc acccatatcc aggtgtcaac agcgtagccc tagcggctgc gggcatcccc
      841 gaagaagagt gggacgacgg tcttctcctt gagaagaggg ggaacccggt catcaaggat
      901 cgatcgcctg acggcactga tggcatcctc tgccttggcc ttcacatcgt agacaacaag
      961 ttccagtgga cgaccgagtg ctcctccggc cgcgtcgatc tactccaatg ctatgatcgc
     1021 gccattcttc tcgtggactc cccgcgtcgc ccgtcagatt cgcaacataa cctttcttga
     1081 taggctctgc ggcaaatgct acgggggaca gaacaaataa gaccaatggc gcagtaataa
     1141 cccttttctt caccaacgat acactccctt tatggatttt gagtataggg tcggtttttg
     1201 agtgtcaata tagcaattta actctacaaa tggcatgcaa caaaatgttt gacatggtat
     1261 tgcccgtcgc aaaagtagaa agaaaaagca agatcacgac ctccaactaa ggtctgacct
     1321 cagataacct ctcaatttct tgacctatgg tgactgggat ttccggggtt atggagagag
     1381 caacgggtcg tgctcctgag tccagttgct tttccggcgt gtatgacttc tgctataccg
     1441 gaggaaggtt gtggatcccc gctctcgtcg ccggaccgca ggggggcaac ttccacgccg
     1501 ccgaagaatt tgaggagaca gacgacatta cctgcagatc attgatccat gcaaaacgtt
     1561 acggaactgg tcctggagct actccaggac cagttccatg aatgaacggc tgtccatccc
     1621 tgaaatgtaa tggctcacat ctacccgttt cagcatcccc gaaaggccct tctcggaaaa
     1681 ctgcgttccc gcaagaccat tctcaaggcc ggcgatatcc ccgttgccga aaaagtcccc
     1741 gaagatcctg caggaggtta tccttccctt tcggacgttg agcctgacat ccaccttccc
     1801 gaagccgaaa cgtctttcct tccgaaggtc gaactccggt gaggccccgt agttccagtc
     1861 ccaggtcccg tacttgctct ccgcgagggc cgcggccttt tcctgcaccg atgggtccgg
     1921 cggggagatc tcaagaccgg gccaccgctc ctttacagtg aaaaggagcg ccctcctgaa
     1981 aacttcaata tcaggttctc ccttgaggtg ggaccttatg ttggtcacac ggcttcttgc
     2041 cgacttgacc cccttggatt tgaacttgtc cgggtctgcc tggagcacct cttcgagagc
     2101 gtccatatcg ctgtcgtaaa ggatggtgcc gtggtgaagg agtctcccct cacgcatgta
     2161 ctgggcattg cccgagaact tcttgccctg gatcaccagg tcattcctgc cgaccaggcc
     2221 tccgtccacg cccaggctcc agagggccga tattaccggt tcagtcaatt tcctgaagtc
     2281 caggttctgg gggttttcgg tcctgaggat aaaggtaaag ttcagatttc ccaggtcgtg
     2341 gtagacggca cctccaccgg acagacgccg taccacgtgg atgcccttct ccctgaccac
     2401 ggggacgttg atctcttcaa gggtgttctg gaacctccca accactacgg taggctcatt
     2461 ctgccagaga aggaagaagc cctcctcttt ctcatctaag gactgaaaga gcacctcttc
     2521 aagggcaagg ttgaacctgg ggtcggtctt ccggttctcg atatatttca ttggcccctc
     2581 cccattccag gtaccgacac tgctcagacc cggggtatgc tgtgaattgc cctttcaaga
     2641 ccggcaagga cagcttctct cgtagcttca cagaacgtgg gatgaccatg gatcgtttcg
     2701 gccacttcgt ccagggtggc ttcgagcctg atggcgaggg aagcctcgga tatgaggtcc
     2761 gtggctctcg cgccgactat gtgtacaccc aggatctctc cgtacttctc gtcggcgact
     2821 atctttacca ttccccgggt ctccccggag atcaggctct tggcgttccc tatcattggg
     2881 aagacaccct ttcttacctt atgacctctc ttcttcgcct cttcctcggt aaggcctgcc
     2941 caggccagtt ccggtgaggt gtagacacaa cctggggtag tgctgtagtc catcttctca
     3001 tcgaggccga aaatgttgcc cgcagcgacc tctccttcca tcattgccac gtgagccagc
     3061 atgatcgggc tgcagcagtc acccacggcg aagatatggg gcacacttgt gcgcatggac
     3121 ttgtcgacgc tgatcctgtc cctctcgatc cttattcctg ccgcctccag gttcaatcct
     3181 tccgtgaaag gcttgcgccc caccgccacg aggaccttgt cggctccgat cttctcttcc
     3241 ctgccggaaa gatcggcaga aatgaccagc gatgacccgg ctgcctctat ggatccaacc
     3301 tttgccccgg tgtgaattgt aatacccctt gcctcgagaa ccttcctgag ggcggaaaca
     3361 gcttcaccgt ctgcacccgg cagtatctgg ggcagcattt cgatgatcgt cacgctgcat
     3421 ccgaaatcgg catatatgga ggcaaattct acacctatca caccgccacc gatgatgacc
     3481 atcctcgatg ggatcttatc gaaagagagg gctccagtgc ttgttactac accaccgagc
     3541 tccacgccgg agatgggggg aagggatgcc tccgaaccag tggcaacgat cacggcatca
     3601 ctctcgatct tcctgtcacc ttccccggac cggaccagga tgttcctgtc atccatgaac
     3661 gaggcttccc cagtgatgat ctcaacgccg tgagccgata gaagcgcctt aatgccctgt
     3721 gagagctgct tcacgacctg gtccttgcgc tcctggacga cgtcccattg gacttccaaa
     3781 gaacccacct tcaaacctgc ttcggcggca tcttcaaccg tacgattcac ctccgccgag
     3841 tgaagaaggg ccttggtggg tatgcagccc acgtttacgc aggtacctcc cagcctctct
     3901 ttctccacca gggtgacatt gccgcccagc tgggctgctc taatggcagc tacatatccc
     3961 ccggggccgc cgccaatgac caccacccgt ttacctgtgt ctcccttcgc cggagcgggt
     4021 tcggtaccct tcaatgaacc agtgggccgt tcttgctctc tttccgaagc ggcctcatct
     4081 ttcgaggttg ggacctcttc tgctacggcg actccgagat ctggctcttc ccccttctct
     4141 ccgatagcac ccaggatcgc cccgaccgga acggtctcac cggccttgac ggctatcctg
     4201 aggagcaacc cgtcatcggg ggcctcaact tcgtaggtaa gcttctccgt cgctaccaca
     4261 agaacgacct cacccttctt cagggtatcc ccctcattct tcttccattc caggacggaa
     4321 ccttccgtca tcgtaagccc cagcttgggc atggtgatca tagtcgccat cagttcacgc
     4381 tccatttatc tgtctgccac tcctggacaa gggtcatcca gggtttttcc acaagctccc
     4441 tgacccgggc aaggaacctg gctgcatcgg ctccatcgag gatcctgtga tcggccgtaa
     4501 ggcctatcat tgacatgggt cttactacca cctgcccgtc cctgcagacc gggcgttcgg
     4561 tgaccacgtt cactgccagg atgcaggcct cgggtgggtt gatgatgggg atgaaatcat
     4621 ggatcccgaa catccccagg ttggtgatcg taaaggtccc cccctgcatc tcttccggga
     4681 gaagcctgct cttcctcgcc ttgttgacca gctccagcga ttccatggcg atcacgggca
     4741 agggtttttc ctgtactttc ttgacgttcg ggaccagcaa tccaccttct acggcaacag
     4801 ctatcccgat gttgatatcg tcgtgcagga tgtactcgtt ccgcccggga tcgtatgagg
     4861 cgttggccat gggcatctcc accagggccc tggcgcagat cttcataagg atatcgttga
     4921 aggagacctt gaccccgctc ccttcaagct cctcgttcaa ggccttcctg aaaccgatga
     4981 actcggtgaa atcggtctcg atgttgaaga agaccgacgg gatggtcgaa gcgctgaagg
     5041 tcatcctctc ggcgatgacc ttccgcatcg gggtgagagg aaccctccta ttcacagttt
     5101 tactctcttt cgctgcacag gctgcctcga gcacgtcgcc cttcatgatc ctgccggaca
     5161 ccttgatcgt ccccagaggt acttccaggt cttccgccag cctagcggcc acaggggaga
     5221 ccctgatcct ctccttggga ggtgaagcaa ggtatgcctt cacatctccg gcggtggttc
     5281 gacccatcgg gcctgtccct gatatctcct gaacgctgat accagcctcc cttgcgatct
     5341 tccgcgccaa cggggtagca cgggaccttg tcgaggcctt cataacatcg tccttcacta
     5401 cccggccacc gggaccgcta ccttcgatag cgccgatgtc gagcccaaaa gaccttgccc
     5461 acttccttgc gaggggtgac gccgcagctg tctgcccctt gctttctggc ccgggaacat
     5521 cctcattcgt tccaactcct ctggacagag gtgtcgaggt tgagggtcgc tccactgcct
     5581 cttctggccc aggttcgggc ctctcccctt gccgatccgc ggcagatacc ttttctcccg
     5641 cctctcccag gaccgccagg actttgccaa cgggaacact ctcgccctct ttgaccagta
     5701 tctcaagtac gacaccatcg ataggtgacg tcacatcgta ggtcagcttg tcggtggcaa
     5761 cgaccaggag cacttcctcc ttttttatcc tgtcaccagg ggagaccttc cacttcgaaa
     5821 cggtaccctc cgtcatggtc aggccgagct tgggcatggt cacctgttcc atcggttatc
     5881 ctcacatgtc attaccggga gtcctctcag aagagtcccc ggatcgcttc ggcgatcttc
     5941 tcgggggtga ccctgtaggc ctgttctaga ggccgggcaa agggcaccgg agtaaagggg
     6001 gctcctaccc taacaatggg ggcgtcgagg aagtcgatcc cctcttcggc tactatggcc
     6061 gatatctcgg ctcccacacc tccctgttta accgcttcgt gggcaataac aagcctggtg
     6121 gtcttggcca ccgaggcaag aatggtttcc ttgtcgatag gagagatggt cctgaggtct
     6181 atgcactcca ccgatacatc ttcgccggca aggatatccg ccgccttcag ggaaagaccc
     6241 atgcagtgag agtaggatac aagggtcaca tcgctaccct cccttgccac cttggccttg
     6301 cctatagggg acaacacctc gccgtcaggg acagctccgc gggtgttgaa aagggccttg
     6361 tgctcgaagt agatcactgg actatcgtcc cttatggcag atttcagaag ccccttcgca
     6421 tcggcagggt tggagggtat gaccaccttc agccccggga tgtgcatgaa ccaggcctct
     6481 atgctctggg agtgttgtgc cgctgcctgg ttgataagtc cgtcaggagc cctcagcacc
     6541 atagggacag acctctggcc tccgaacata tagtgtatct tcgccatctg gttgaatatc
     6601 tcgtccatgc agacgcccat gaaatcggca aagtgcatgt ccgctacagg tctcatgccc
     6661 gtcagggcag ctcctatggc cgctccgacg atggccgtct cacttatggg agtgtccttc
     6721 accctgtcct tcccgaactt gtcgggaagc cccttgaact gtccgaagat cccaccctgc
     6781 cttgcgatat cctcacccat gacgaaaacc ctttcatcac gggtcatctc ctcgtccata
     6841 gcttccagcg tggcctgtga gaacgtgatc tctctcatga tcctttcacc aacccatcct
     6901 tacgcgtaga cgcccgcgaa gagttccgaa atttccggct cctcggcatt cctggcgaac
     6961 tcgagagcct tttcgacttc cttaccggcc gaggctttta tcgcatcaag ctcctccctg
     7021 ctgaagagct tgccgtccag gacggtgcac tcgaacctgt cgatggggtc attgctctcg
     7081 cagaccttgt taacttcttc ctttgtcctg tatcgctccg gatctcccac atagtgtccc
     7141 tttacccggt aggtcttgca ctccagaagc gacgggcctt caccccttct cgcccggtcg
     7201 atcagctttt gcgactcttc gtatactgca aatatgtcgt tcccgtccac tattaccccg
     7261 ggcatcccgt agcccgctgc cctgtcggca atgttctcga cggaggtagt ggtcatgtag
     7321 ggagtggtag aggcatactg gttgttctca caaacgaaca gtacgggcag gtcccagact
     7381 gcggccatgt tcgcggcctc atggaaggta ccgcgattgg aggccccgtc accgaaaaag
     7441 accaccgata cgttgttctt gccctgcatc tttgtggcca gagctgcgcc ggtggcgata
     7501 ttgaaaccgc ctccgacaac gccgttggct cccagcatcc cgatgctcag atcggcaata
     7561 tgcatggagc ctcccttccc cttgcagtat ccttccttct ttccgaagat ctcggccatc
     7621 attctcttca gatcggcacc tttggctatg gtgtgtccgt gaccgcggtg agtgctctgg
     7681 atataatcct ccctggagag gttcgagatc acccctgctg cgatggcctc ctcgccgatg
     7741 taaagatgaa cgaaacccgg tatctcgccg gccaggaact tttcctccac ctttttttcg
     7801 aaagacctta ttgtaagcat ggtcctgtag aactgctcga gaagcgtcct ggggtacttg
     7861 ttcaggccct tcttctcctt aactttcttt gcgctcaagg atcccccacc cctcttcctg
     7921 aaaatgatca tccggtcctg ttgccgtgta tgatcttgtt gttcgggaca ttcactaacg
     7981 catcagcagg ttcggcagcc aaagggtaat gcccggcagg tagacgatca ccgccagggc
     8041 cgcgatagaa gccagaagga agggaaccac cgccctggat atttcgtcca tggagacccc
     8101 cgccacattg cagccaacgt acaggttgac cgccacgggg ggcgttacct ggcctatggc
     8161 aaggttgatg gtcataacca ccccgaacca gaccgggtcc caaccgaaat gggcgatgac
     8221 gggcatgaaa atcggcatca gaaggtaata gatagatatg gcgtccagta gcatccccga
     8281 tatgatcagt attacgttga tcagggctat caccacgaag gggttggaac tcatggctat
     8341 gactccctcg gagaccctct caacaacccc gaggaccatc atggcccatc cgaagatccc
     8401 ggcgaaggcg actaccagca tcaccaccgc tgaagaagcg caggcgtcca caaggagttc
     8461 gtaaagatcc ttgagggtga gcgtacggta gacgacaaag cctacgaaaa ggccgtaggc
     8521 cactccaaca gcagcggctt ccgtgggagt gaagatcccc ccatatatcc cccccaggat
     8581 tatcactgga gtgagcagag cccagaaggc atccttgaag gccgttagga cctcctttgc
     8641 cgttccgaac ctgtcgccct tgtagcctcg cgctacggag atgataaagg cagcgactat
     8701 aaggaagaga cccaccagga tcccgggaat tatccctgca aggaacagtt cgctgatcga
     8761 ggctcccgtg ataacaccgt agacgataaa gccgatgctc ggagggatca ctatggccaa
     8821 tccgcttgcc gatgaggtca gggcggcagc gaaacccctg tcgtaaccgg ccttgaccat
     8881 gcccgggatc agaatggtgc caagggcggc gacggtagcc ggccccgaac ctgaaacggc
     8941 tccccagaag agggccgtga ttatcgtcac aattgccatt cctccgctca cgggtcccac
     9001 cattaacatg atcagcctga tgatcttgtc tgagatcttc gccctcccca tgatgacccc
     9061 ggcgaggatg aagaatggaa tggccagtag cgagaatttg gagatgttgg caaagaacac
     9121 gggagcgaac atctgtgctc ccaggttgta ccagtagacg acggccatcc ccgccatgcc
     9181 gagagcgacg gctatgggta ccttgaaggc aaggaagata aagaaaaggc ctaacagaaa
     9241 aacacccgga tctatgctca tctttggtcc acctccgggc aggaccccgt ctgtggacca
     9301 gacaggtcat tccattcatg tacgcccctc tggatcgttc tcaggatcag gataaaggca
     9361 cagacaggca cggccattcc gaagatccac ataggcagcg ccatggagta cgtggtcatt
     9421 ccggacctga actcgctctt gaccatatcg atcccatgca tggtgagaag gccgaaaagg
     9481 gtgactccgc agaggacgaa taccgcggtc gataacttct tcagcgacgg gggcatccgg
     9541 tcaaagagat aactgacgcc gatcagtgac cccttccggg tcgcgatcga agctccgagc
     9601 atcgttatcc agacgaagag gttcacaagc agctcctccg agaaggagaa agagaaaaca
     9661 gtcaggtacc ttacgatcac attgacaaag gccagcatgg ccatgaagaa aagcattgtg
     9721 acacaggtcc actcttcgat cctgtcaagt aacttgaaca tccttagcac cgttcccttc
     9781 ctatcggatg atccccgaca gatgcctctc ccgggacggg aacaggggag ccgctcctgc
     9841 ggctccccct agagcatggt atgttattta aagctggaga cagcctcctc gaaggctttt
     9901 acgatatcgg caccgacatt tccggaccac ttctcataga ccgccttggt catttcgcgg
     9961 aacgcggcaa gctgctccgg agtaggatcg gtcactgtca tcccctggga tttgaggaac
    10021 tcgatgccat ctcccaggcc tacacggctc acgtcgtgca cctgccagtg catggcatcg
    10081 acggcggcct tcttgatctg ctccttgata tctggatcga agctctccca aacgttcttg
    10141 ttaacggcca ggataagggc gtcgtaggaa tagtgccagt tggtgaggtg cttctggact
    10201 tcgtatatct tgttcgggat tatgatcccg acaatgggg
//
LOCUS       DAOPKQ010000024        10186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_6920_length_10186_cov_4.288915, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000024 DAOPKQ010000000
VERSION     DAOPKQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10186
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6920_length_10186_cov_4.288915"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..727
                     /locus_tag="PK146_01695"
     CDS             47..727
                     /locus_tag="PK146_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HPE90982.1"
                     /translation="MLPVLDEEIARHRIARRVAMEFQDGDVVNLGIGIPTLVSDYIPE
                     NIRVIFQTENGVVGAGPKPENEDLRFIGAGGRCLKPIPGGALVASDMSFGLIRGGHLD
                     ATVLGALEVDQEGNLANWWIPGKMVPGMGGAMDLVTGAKHVIVATTHCTKRGDPKILP
                     KCTLPLTGAKVVSLVVTEYGVLRMDSGEMALEEIAPGVNLGEFKKVTPANYRVSDDLC
                     QMKGCELE"
     gene            830..2011
                     /locus_tag="PK146_01700"
     CDS             830..2011
                     /locus_tag="PK146_01700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPE90983.1"
                     /translation="MKRPVILAACRTGGGKFGGVLSGFEATDLGGFAIRAALDRSAVP
                     EEEIGEVIMGNGWQAGVGANPARIAMFKAGMPRSVPAFTVNKRCGSSLRTAMLIADRI
                     RLNDIRVGVAGGMESASRTPYILPEARWGHRMGDRKVLDALHHDGFMCPLAGMLMGAT
                     AEILAEEYSITREEQDSYALESHRKAFSAMKKGLFADETFPVSIRQKKEVVEWASEEI
                     PREDTSLEKLARLPAIFKDKGTITAGSSSALCDAGSAAIIADPDWAKANGFNPLAEIA
                     GYGSGALEADHMGLGPVKAVPRAMEMSGMTLGDMDLIELNEAFAAQVLAVHRAMPFDM
                     ERCNVNGGAIALGHPIGATGTKILATLIYALRKRDKEFGLATACIGGGQGVAMVIRRL
                     S"
     gene            2346..3461
                     /locus_tag="PK146_01705"
     CDS             2346..3461
                     /locus_tag="PK146_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE90984.1"
                     /translation="MKIEEFRLERTLARFQNEVEYDLSASGIYPMFIREILSPGEMEE
                     VYNDLHLKYVHTAGTEHLRKAVASFYEGMDPSRVFMTNGSAEALFLMAWGLIEPGDEV
                     AFMIPNFMLLSHVVESNGAVVRDFHLDPDRGWSLDLDSLKKAVTPKTKLICVCNPNNP
                     TGTVLSRDQMKQVVEIASEADAYLLSDEVYRGAEQTDEMTPSFWGMYDKVIVISGLSK
                     SFGLPGLRVGWVAGPSEIADMSWFYHDFLSTTITTFSDYLATKALEPGMREKIFSRNR
                     GIVRQNLATFTDWVNSHGKALSFTPPQAGCFAFVRYDLPVSSWDLVMDMVRTGSVFVI
                     PGSCFGIENHLRMNFGVDERRLTEGLERIDRTLSKYI"
     gene            3487..3738
                     /locus_tag="PK146_01710"
     CDS             3487..3738
                     /locus_tag="PK146_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPE90985.1"
                     /translation="MRCSDGTYYCGIAMDPQKRLRQHNDGKGARYTASRRPVDLVYAT
                     SCRYSRSQAQAIERKAKSRPRDLKLSFLEKADAEVGTET"
     gene            3771..4679
                     /locus_tag="PK146_01715"
     CDS             3771..4679
                     /locus_tag="PK146_01715"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPE90986.1"
                     /translation="MSKSREIRKVIRGRPVVEGAGVHLRRVFGFGETKDFDPFLLLDD
                     FRSDDPRKFLPGFPWHPHRGIETVTYMIEGTVEHGDSLGNSGVIGPGEVQWMTAGSGI
                     IHQEMPKGDEGGRMGGFQLWANLPASHKMMDPRYRDLGRDRIPVIRKNGAKIRLICGE
                     IEGIRGPVTDVMTDPGYLDLEVPAGVSLSVHVPGGHTAFAYVIAGRAFFGEEREALSF
                     ELEGENYFDMNARPFISDGELALFDDGEKVDIRAGEFPARLLLFYGKPLGESVAWRGP
                     IVMNTREELQEAFEDLQRGTFIRNSG"
     gene            4750..5451
                     /locus_tag="PK146_01720"
     CDS             4750..5451
                     /locus_tag="PK146_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HPE90987.1"
                     /translation="MKRSVYKRVVSSLVTLSLLLLLAAPVLAATANERITSSVHVVRD
                     MSKEDDVGAMADLVKSAKGVVIIPNYVKAALGIGGAHGEGLVLRRDPATNTWFGPSFL
                     SVTGASIGFQIGVQSTALLLVVTNQKGMDHFYGDKFKLGADVDIAAGPVGRSAGAATD
                     VNLQASIYSYSISKGLFAGLSLSGAMMTTDHDTNSSYWGTSINSRDILQKKASAASIQ
                     PLLSALNDLIRRSGQ"
     gene            5637..6389
                     /locus_tag="PK146_01725"
     CDS             5637..6389
                     /locus_tag="PK146_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPE90988.1"
                     /translation="MNSHEKLIWILKRLGEPPYEMGVTELSREIGVSKSGIHKILKSL
                     AEENLLVQNPSTRKYSLGPLIFRLGNVYSDSKGIWEVSHTIMDSLAKETGTAVLIGIR
                     EGDDPILAYKMGTDEKFIYQGQIGTRFPFNAGALGKLLTAFLPEDRIRSILQTRSIER
                     KTPYTETDPDRLMEEYRRIRRQEYAVSIEENRVGAFGMAAPIRDRHGKVWACLSLVGP
                     RETFTPDVVDNWISLLRTGADEISCRLGFRGA"
     gene            6419..7576
                     /locus_tag="PK146_01730"
     CDS             6419..7576
                     /locus_tag="PK146_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HPE90989.1"
                     /translation="MVIQNYISSLLSNPIFLLSVAVFTGLVLGSVKIKGVGLGTSGAL
                     FTGLLMGWLGVKVPSVFFMWNLLIFVTAVGLLSSKDIVGVIRKYGYRFIILSVAVTGA
                     GALATYFMALVFSGSVEPLLVAGTYTGALTSSPGLGAALEISGGNPLITIGYTIAYPF
                     GVISVVLFVQLLPVLFKVDLVRERELLIRAIHDTPPGSAGGVSSVPFTLASFVLCMIG
                     GVLFGTISIPIPLVGDFSLGSTGGALIFALFAGALGSIGPFPMRLDTSVLSALRTFSL
                     AYFLAVMGIQAGPDIPRILAEHGVALVAIGAVSAVVSLIVGWIVGRGFFKLNWVVFLG
                     AIPGAMTSTPGLGAAIESTGSDECSAGYGATYPAAIFCMVLFTKLIIHALR"
     gene            7677..8741
                     /gene="ord"
                     /locus_tag="PK146_01735"
     CDS             7677..8741
                     /gene="ord"
                     /locus_tag="PK146_01735"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HPE90990.1"
                     /translation="MLTSKGRMDNAVPDRKRVVVAGLGAMGSGIARLLLDKRALVQVV
                     GAGAARPDKHGRDLGEVLGTGEMTGVAVTSIGELADIEADIVIQATTSFTREAFPDIM
                     QFVRSGKNVISIAEEMSYPHVTEPLLAKDMEEAARSNGVTILGTGVNPGFILDTLILT
                     LTGSFGNVLSIRASRINDLSPFGHGVMKTQGVGATPEEFRKGLDEGSITGHIGFLQSV
                     HMIADALGWDLDEVIETREPIISRVHRKTPHVTVEPGMVAGCNHSVKGMSKGEPVIVL
                     EHPQQVHPQLEGVETGDYIKIQGDSSSVDMAIRPEIPGGIGTISMAVNMIPQVLEAPA
                     GLVTMLDLPFPRAFMKIKVR"
     gene            8850..>10186
                     /locus_tag="PK146_01740"
     CDS             8850..>10186
                     /locus_tag="PK146_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPE90991.1"
                     /translation="MSAANNDTVKKFKVPHTYVILFIIIVLATIGTYFIPAGVYERVK
                     DAATGRTIVDVASYHNVEQTPVSFFGMFKAIPQGMIRAASIVFFVFIMGGSFTMMQAT
                     GAIDAGIAKVITRFKDRDKLVIPVIMFLFSILGFTIGAAEEVIPFVPIAIALSRGFGY
                     DDIVGVAMVSTGAAIGFSGGMLNPFTVGVAQGIAELPLYSALAFRSVVYVILYVVAVW
                     YVLRYAAKIKADPTQSVLYGVERDAIATSDHDEMPVMTGRHKMALLAMVVGIGFMIYG
                     VMKQGWYINEISALFILLGIVVAFIGGLGANDMAKSFVQGCRAIAFGALVVGIARVIL
                     VVMEQGQIMDAIIHGLASAVGMLPTQITAVGMFVVQSIINFFIPSGSGQAATVMPIMS
                     PLADVLGITRQTAVLAFQYGDAFSNQIIPTSGALLGILGLANVPYDRWLKYNW"
BASE COUNT         2245 a         2693 c         3020 g         2228 t
ORIGIN      
        1 cccggcattc tcgtggatat cctggttctg aagggggatt cctactatgc ttcccgtact
       61 tgatgaagag atcgcacgcc atcgtatagc gcggagggtg gcgatggagt tccaggacgg
      121 agacgtggta aatctcggga tcggcatccc tacactggta tcggattaca tacccgagaa
      181 catccgtgtc atatttcaga ccgagaacgg tgtagtaggg gccggaccaa agccggagaa
      241 cgaggacctg cgtttcatcg gagccggagg gagatgtctc aaaccgatcc ctgggggggc
      301 cctcgttgca agcgacatga gcttcggcct gatcagggga gggcaccttg atgccactgt
      361 ccttggtgcc ctggaggtgg atcaggaagg caatctggcc aactggtgga taccgggcaa
      421 gatggtcccc gggatgggtg gggccatgga tctcgtaaca ggggccaaac acgtgatagt
      481 tgccacgact cactgtacaa agaggggaga tcccaagatc cttcccaaat gcaccttgcc
      541 actgacggga gcgaaagtgg tgagccttgt ggtgactgag tacggtgttc tcagaatgga
      601 ctcgggggaa atggcgctgg aagagatagc cccgggcgtg aacctcgggg agttcaagaa
      661 ggtaacccct gcaaattaca gggtctcgga cgacctgtgc cagatgaagg gctgtgagct
      721 ggagtagaag acaggttccg gggtggtcgg aatttcccgg tcaacagggt ttaatggtcc
      781 cgaaagaatc tttggggccg atcgaagata tatccaggag gcgttttgaa tgaaaagacc
      841 tgttattctc gccgcatgca ggaccggcgg gggcaaattc ggcggagttt tgtcagggtt
      901 cgaggcaaca gaccttgggg gctttgctat cagggcagcc ctggacagat ccgctgtgcc
      961 ggaagaggag ataggcgaag tgataatggg gaacgggtgg caggcaggcg taggagccaa
     1021 cccggccagg atcgcaatgt tcaaggcggg gatgccacga tcggtaccgg ctttcactgt
     1081 caacaagagg tgtggttcca gccttcggac ggccatgctg atagccgaca ggatccgcct
     1141 caatgacatc agggtcggtg ttgcgggagg gatggagagt gcctccagga ccccctacat
     1201 actccccgaa gccaggtggg gtcacaggat gggcgacagg aaggtccttg acgcgttgca
     1261 tcacgacggt ttcatgtgcc ccctggccgg aatgctcatg ggggctaccg ccgagatact
     1321 tgcagaagag tacagtatta caagagaaga gcaggattcc tatgcgctgg aaagccacag
     1381 gaaagccttc tccgcaatga agaagggtct tttcgctgac gagacctttc cggtctctat
     1441 caggcagaaa aaagaggtcg ttgaatgggc ttcagaggag atcccgagag aagatacaag
     1501 tctggaaaag cttgcccgtc tgccggccat attcaaggac aagggcacca ttacagcagg
     1561 aagcagctcg gctctctgcg acgcgggaag cgctgcgatc attgcagatc ccgactgggc
     1621 caaagccaac ggattcaacc ccctggcgga gatagcaggc tacggcagcg gcgcccttga
     1681 ggcagatcac atggggctcg gacctgttaa agccgttccc agggcaatgg aaatgtcggg
     1741 tatgaccctg ggggatatgg accttattga actcaacgaa gccttcgcag cccaggtcct
     1801 ggcagttcat cgtgccatgc cctttgacat ggagaggtgc aacgttaacg ggggagcaat
     1861 agctctcggt catcccatcg gagcgacagg caccaagatc ctggccacac tgatatatgc
     1921 actcaggaaa agggacaagg agtttggcct tgctactgcc tgtattggag ggggccaggg
     1981 cgtggccatg gtaatcagaa ggctctcgta agcgccggct tgaaaaagat cagggccgcc
     2041 tccgggcggc ccgttttttc atggagttac tccttatcct tgccgcactc accaccgcaa
     2101 caggggttgt tcttttcctt cggatcttcc ttcttttcac agcagttatc ctgtttctgt
     2161 tgttcttttg tctcctttgg ctttctgcag caacccacag ttactccccc ttttcttgtt
     2221 cttcaatgca attataccct tggtggtatt tatttcaaaa tctttttata ttattgtttc
     2281 cctccggggg aaggaggtag aatgcgggta tagatccaac attccagtca gcaggaggcc
     2341 tttagatgaa gatagaggag ttcaggctcg aaaggacact ggccaggttc cagaacgagg
     2401 tggagtacga cctcagcgcc tcggggatat atcccatgtt catacgggag atcctctccc
     2461 ccggtgagat ggaagaagtc tacaacgatc ttcatctcaa atatgttcac accgccggga
     2521 ccgagcatct caggaaggca gtagcctcat tctacgaagg catggaccct tccagagtat
     2581 tcatgaccaa cggatctgcc gaagcacttt tcctgatggc ctgggggctt attgaacccg
     2641 gtgacgaggt ggctttcatg atccccaact tcatgctgct ctcccacgtg gttgagtcca
     2701 acggggccgt ggtccgcgac tttcacctgg atcctgatcg aggctggtcc ctggacctgg
     2761 acagccttaa aaaagccgtt acaccgaaga cgaaactgat atgcgtctgc aaccccaaca
     2821 accccaccgg gacggtcctt tccagggatc agatgaaaca ggtcgtggag atcgcttcag
     2881 aagcggatgc ctatctgctc tccgacgagg tgtacagggg agcggagcag accgatgaaa
     2941 tgacgcccag tttctgggga atgtacgaca aggtgatagt tataagcggc ctctcgaaat
     3001 ctttcggact acccgggctt cgggtaggct gggtggcagg tcccagtgag atcgcggata
     3061 tgtcctggtt ctaccatgac ttcctctcca cgacgatcac cacctttagc gactatctcg
     3121 caacgaaagc ccttgagccg gggatgcgtg aaaagatatt ctctcgcaac aggggtatcg
     3181 taagacagaa cctggccact ttcaccgact gggtcaacag ccatggcaag gcgctttcct
     3241 tcacccctcc ccaggcgggt tgtttcgctt tcgtaaggta tgacctccct gtatcctcct
     3301 gggatctggt gatggacatg gtaaggacag gcagcgtatt tgtcattccg ggaagctgtt
     3361 tcggcataga gaaccatctg aggatgaact tcggcgtcga tgaacggcgc ctgaccgaag
     3421 ggttggagag gattgacagg accctttcaa aatatatctg aacgaggtgc tttctgcgtt
     3481 taccttttga gatgctccga cgggacctac tactgcggaa ttgccatgga cccgcagaag
     3541 cgcctccggc agcataacga cgggaaaggg gcgaggtata ccgcttcgcg gaggcccgtg
     3601 gacctggtgt atgccacctc ctgtcggtat tcacggtccc aggcccaggc gatcgagaga
     3661 aaggccaaaa gcaggccaag agatctcaag ctctcctttc tggagaaggc cgatgccgaa
     3721 gtcgggaccg agacctgatg caagacaggt gacggaaggg ggacccggtg atgtccaaaa
     3781 gcagggagat caggaaagtg atacgcggaa ggccggtggt cgagggggca ggggttcacc
     3841 tgaggcgggt tttcgggttc ggcgaaacga aggattttga tcctttcctg cttctggacg
     3901 attttcgttc cgacgatccc cgaaaattcc tgccgggatt cccctggcac ccacacaggg
     3961 gtatcgagac cgtcacgtac atgatcgaag gcaccgttga gcacggtgac agccttggca
     4021 actccggggt catcggtccc ggtgaggtcc agtggatgac tgcagggagc ggtataatcc
     4081 accaggagat gcccaagggc gacgaagggg ggaggatggg ggggttccag ttatgggcga
     4141 accttcctgc ctcccacaag atgatggacc ctcgatacag ggacctgggg agagaccgga
     4201 tccccgtcat caggaagaac ggggccaaaa tcaggcttat atgtggagag atcgagggca
     4261 taaggggtcc tgtgacggat gttatgaccg accccgggta cctcgacctt gaggtccccg
     4321 ccggggtctc cctgtccgtt catgtccccg gaggacatac cgcctttgcc tatgtcatcg
     4381 ccggaagggc ctttttcgga gaggaaagag aggcgctttc attcgaactg gagggggaga
     4441 actacttcga catgaatgcc cgtcccttta tctccgacgg agagctggcc cttttcgatg
     4501 acggagagaa ggtagatatc agagcagggg agttcccggc gaggctgctt ctcttttatg
     4561 gaaaacccct cggggaatcc gtggcatggc ggggccccat agtcatgaac accagggaag
     4621 aactccagga ggccttcgaa gacctccagc ggggtacttt cataaggaac tcgggatgac
     4681 ccccttgcca tatgtattaa tatatcagag tataatccgg acagaaaaca ggcaaaggag
     4741 gtggcggtca tgaaaagatc cgtctacaag cgagttgttt cctcgctggt tacgctgtca
     4801 ctgctgctcc ttttggcagc ccccgttctg gcggcgacgg cgaacgagag gatcacctcg
     4861 tcggtccatg tggttcgcga catgtcgaag gaagacgacg tcggggccat ggcagacctg
     4921 gttaagtccg ccaagggggt agttatcatc cccaactacg tgaaagcggc cctgggcata
     4981 ggaggagccc acggagaagg gcttgtcctg agacgtgacc ccgcgaccaa cacatggttc
     5041 ggacccagtt tcctcagcgt tacaggtgct tccatcgggt ttcagatcgg ggtgcagtca
     5101 acggcccttc ttttagtagt gaccaaccag aagggcatgg accactttta cggcgacaag
     5161 ttcaagctcg gcgccgatgt cgacattgcc gcaggtccgg tgggacgaag tgccggcgct
     5221 gcaacggacg tcaaccttca agcctcgatc tacagctatt cgataagcaa ggggctcttt
     5281 gccgggcttt cgctgagcgg ggccatgatg accaccgacc atgacaccaa ctcctcctac
     5341 tgggggacat ctatcaactc cagggacatc cttcagaaaa aggcttcggc agccagcatc
     5401 cagcccctgc tgtcagccct gaacgacctc atcagaagat caggccagta agtcgatttt
     5461 cggttcgaaa agtgcggtaa cccccaggcg ggaccttggt cccgcctttt tcctcccttg
     5521 cgaccgaaag gtcattttac atacaacccc ttgacaaaag tgtccctgtc cgaaagaata
     5581 attattaagt tctgtaggag agaaacaggt tctctgtcag agaacggagg cgcgggatga
     5641 acagccacga gaagcttatc tggatcctga agaggcttgg agaacctccc tacgagatgg
     5701 gggttacgga gctctccagg gagatcggtg tctccaagag cggtatccac aagatcctca
     5761 agtcacttgc cgaagagaac cttcttgttc agaacccctc caccaggaag tactcactgg
     5821 gcccactgat cttccgtctc ggtaatgtct atagcgattc caagggtata tgggaggtat
     5881 cccacaccat catggattcc ctcgccaaag agacaggtac agccgtactt atcggcatac
     5941 gtgaaggcga tgacccgata ctggcctaca agatgggcac cgacgaaaag ttcatctacc
     6001 agggtcagat cgggacccgg ttccccttca atgcgggggc cctggggaaa cttctgaccg
     6061 cctttttgcc cgaggatcgc atacgcagca tactccagac cagaagtatc gaaagaaaga
     6121 ccccctatac agaaacggat cccgacaggt tgatggagga gtaccgaagg ataagaagac
     6181 aggagtacgc ggtcagcata gaggagaacc gcgtcggagc cttcggtatg gccgccccga
     6241 tcagggatcg gcacggcaaa gtatgggcct gcctgagcct tgtcggaccc cgggagacat
     6301 ttaccccgga cgtggttgac aattggatct ccctgctcag gaccggggcc gatgagatct
     6361 cctgtcgcct gggtttccgg ggtgcctagg gggaatcatc cttgaaagga caggcgtgat
     6421 ggtcattcag aactatattt cgtcgctgct atcaaacccg atattccttc tcagtgtagc
     6481 cgtcttcacg gggcttgttc tgggcagtgt gaagataaag ggggtaggcc ttgggacttc
     6541 aggcgctctc tttacagggc ttttaatggg ttggctcggg gtgaaggtgc ccagtgtctt
     6601 tttcatgtgg aacctcctga tattcgtcac cgccgtgggg cttctctcat caaaggacat
     6661 cgtgggagtc atcaggaaat acggttacag gttcattatc ctttccgttg ccgtgacagg
     6721 cgccggggcc ctggctacct atttcatggc cctggtcttt tcaggttccg tggagcccct
     6781 gcttgtggcc gggacctata ccggggccct tacgagctcc cctggccttg gagccgccct
     6841 tgagatatcg gggggcaatc cgctgatcac tatcggctac acgatcgcct atccctttgg
     6901 ggtcatctcg gtggtgctct ttgtccagct tctccctgtc cttttcaagg ttgaccttgt
     6961 tcgggaacgg gagcttctga tccgggcgat ccatgacaca ccccctggtt ctgcaggcgg
     7021 agtgagttca gtccctttta cgctggcttc attcgttctc tgtatgatcg gtggtgtcct
     7081 cttcgggacc atctccatcc ccatccccct ggtgggggac ttttccctgg gaagcacggg
     7141 aggtgccctg atctttgccc tctttgcagg agcccttggg agtatcggtc cctttccgat
     7201 gcgccttgat acatctgtcc tttcggcgct tcgcaccttt tcacttgcct attttctggc
     7261 cgtaatgggc atccaggccg ggccggacat accgaggatc ctggccgagc acggtgtcgc
     7321 cctcgtggcc ataggtgcgg tatcggcggt ggtcagcctt atcgtcggat ggatcgttgg
     7381 aaggggattc ttcaagctta actgggtggt atttctagga gccattcccg gggcgatgac
     7441 cagcacccca ggcctgggcg ccgcgattga atccacggga tccgacgagt gctccgccgg
     7501 ttacggggcg acctacccgg cggctatctt ctgcatggtg ctttttacaa agctgataat
     7561 ccatgccttg cggtgatccc ggacaaaggg atctgtctcc tgggaatgca tcagacggct
     7621 agaggatcaa taaagatttt tatgccgcag actaactgag agaaagatgg tggaagatgt
     7681 tgacgagcaa gggaagaatg gacaatgcgg tgccggaccg gaaaagagtc gtcgttgctg
     7741 gtcttggggc catgggaagc ggaatagcca gacttctcct ggacaagagg gctctcgtcc
     7801 aggtggtagg cgcaggggcg gcaaggcccg acaagcatgg gagggatctt ggcgaggtcc
     7861 tcggaacggg tgagatgaca ggtgtggctg tgacatcaat aggggaactt gcggacatcg
     7921 aagcagatat tgtaatacag gcaacgacgt ccttcacgag agaggctttc ccggacatca
     7981 tgcaattcgt gcgatcgggc aagaacgtta tctccatcgc agaggagatg tcctaccctc
     8041 atgtgaccga accccttctt gccaaggata tggaggaagc ggcaagaagc aacggtgtga
     8101 cgattctcgg gacaggggtg aaccccggtt ttatactcga cacgctgatc ctcaccctta
     8161 ccggatcttt cggcaatgtc ctctccatca gggcctcccg tataaatgac ctgtccccct
     8221 tcgggcatgg cgtcatgaag acccaggggg tcggcgccac tccagaggag ttcaggaagg
     8281 gcctcgatga agggagcatc accgggcata taggttttct tcagtccgtt cacatgatag
     8341 ccgatgccct tggctgggat ctcgacgaag ttatcgaaac ccgggagccc atcatctcca
     8401 gggtccacag gaagaccccc cacgtcaccg tcgagcccgg aatggtggct ggatgcaacc
     8461 attcagtaaa gggcatgagc aagggcgaac cggtgatagt ccttgaacac cctcagcagg
     8521 tgcaccccca gctcgagggc gtcgagacgg gagactacat aaagatccag ggggactcgt
     8581 cttcggtgga tatggctatc aggcctgaga tacccggagg catagggacc atatccatgg
     8641 ccgttaacat gatcccccag gttctggagg cgcccgctgg gctggtcacc atgctggatc
     8701 ttccgttccc ccgggctttc atgaagatca aggttcgttg atcccatgga tgatcaagga
     8761 aaggggtgat ggacaaaggt ctttcgctgc atatctcccc agcgggggcg aaatttgaaa
     8821 gggcgatctg aataaggagg ataaggataa tgagcgctgc gaataatgac accgtcaaga
     8881 agttcaaggt acctcatacc tacgtgatac tgttcatcat catcgtattg gctacgatag
     8941 gcacctactt tatccccgca ggggtctacg agcgagtgaa ggatgccgcc acgggcagga
     9001 ccattgtcga cgtggcctcc tatcacaacg tggaacagac tccggtcagc ttcttcggaa
     9061 tgttcaaggc gatcccccag gggatgatca gggccgcgag catcgttttc ttcgtcttta
     9121 ttatgggtgg ctctttcacc atgatgcagg cgacgggggc cattgatgca gggatagcga
     9181 aggtaataac aagattcaag gacagggaca agctggtaat accggtcatc atgttcctgt
     9241 tctccatact cggtttcacc atcggagcag cagaggaggt catccccttt gttccaatcg
     9301 ccatagccct ttcgagaggg ttcggctacg acgacattgt cggggtggcc atggtctcta
     9361 ccggtgcggc catcggtttt tcgggcggca tgcttaaccc cttcacagta ggggtcgccc
     9421 agggcatagc ggagcttcct ctctactccg cgcttgcttt ccggtcggtc gtttacgtta
     9481 ttctctacgt ggtcgccgta tggtacgtgc ttcgttatgc ggcaaagatc aaggccgacc
     9541 cgacccagag cgtcctgtac ggggtcgaga gggacgctat cgcaacttca gaccatgacg
     9601 aaatgcccgt aatgaccggc cgtcacaaga tggccctgct ggccatggtc gtagggatcg
     9661 gtttcatgat ctacggggta atgaaacagg gctggtatat caacgagatc tcggcactct
     9721 tcatcctgct ggggatcgtt gtggctttta tcggcggact cggtgccaac gatatggcca
     9781 agtcctttgt tcaggggtgc agggccattg cctttggcgc cctcgttgtg ggtatcgcca
     9841 gggttatcct ggtcgtaatg gagcaggggc agataatgga tgccatcatc catggtctcg
     9901 cgagcgccgt gggaatgctg cccacccaga taacagccgt agggatgttc gttgtgcagt
     9961 ccataatcaa cttcttcatt ccttccggaa gcggacaggc cgccacagtt atgccgatca
    10021 tgtcgcctct ggccgatgtg cttgggatca cccggcagac ggctgttctt gccttccagt
    10081 acggcgacgc cttctcgaac cagataatcc cgacctccgg cgcgctgctg ggcatcctgg
    10141 ggctcgctaa cgttccctat gaccgctggc tcaagtacaa ctggaa
//
LOCUS       DAOPKQ010000025        10016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_7065_length_10016_cov_4.807851, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000025 DAOPKQ010000000
VERSION     DAOPKQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7065_length_10016_cov_4.807851"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PK146_01745"
     CDS             <1..650
                     /locus_tag="PK146_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPE90992.1"
                     /translation="YINIEDSEEILRAIRMTSSDTPIDLIVHTPGGLLLAASQIAAAL
                     NKHEAKVTIFVPHYAMSGGTLIALAADEIVMDRHAVLGPVDPQIGQYPAVSILKTVED
                     KPVAELDDETLILADISRKAIEQTRRFVASLLVDNIPREEAERLAGLLTEGRWTHDYP
                     ISSEEAAELGLFISTDIPEEICQVMRLYPQSGQRRPSVQYVPMPYHGTTHNTGIK"
     gene            692..1573
                     /locus_tag="PK146_01750"
     CDS             692..1573
                     /locus_tag="PK146_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPE90993.1"
                     /translation="MKSLSRKVLLLAVAVTFAIPALAVAADRGPVITAGEETVSEQEM
                     IRLIMDQSGADKMMAPFVLAQLSLEDRESFAEQVSMALLLSEAALRKGLQLDPSVSVQ
                     LRWNAINILAQAYVASVSSKWDLGRPALEAWFSSHEEHYKEPESVRVRHILVASEKEA
                     VDVLLKVYAKEADFGRVAAENSIDPGSAGSGGDLGWVYRGQTVPEFDSLAFSLEPGRT
                     GGPVETKFGWHVLQVLEKRPARSFTLDEVLPRVREDIQQHYLELEVQRLVDSLGSEID
                     RDILSTLGGFPAYRKDQ"
     gene            complement(1624..2472)
                     /locus_tag="PK146_01755"
     CDS             complement(1624..2472)
                     /locus_tag="PK146_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPE90994.1"
                     /translation="MKGSGSEMIIPVFQVDAFTDEPFRGNPAAVCLVDKFPDEVTMKR
                     IAMEMNLSETAFVVPSKEPEGKGCAAFSIRWFTPSVEVPLCGHATLAASWVIFNERAS
                     SFPRITFESASGKLAAALGEDGITLDFPLNGTEPAEIPDGLLDAIGISSVEDTRFSKS
                     AEMLLLRMTDEAAVRGLQPDFKAMLGIDRGGLGFQGVIITSRSSDFDFLSRYFGPWEG
                     IEEDPVTGSAHTVLAPYWSRITGRSRFRAFQASKRGGELTVAITGDRVKITGRATTVL
                     RGELLL"
     gene            2555..3244
                     /locus_tag="PK146_01760"
     CDS             2555..3244
                     /locus_tag="PK146_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPE90995.1"
                     /translation="MSGVRPMMLESARYEIVLYGRKLLESGLVTGTGGNLSIRSGRFA
                     ALSPSCVEYGLMKPEDVVVVDMDGRVQDGRLKPTSEMFLHLALYLSRPDITAVVHTHS
                     PYACVMACLGREIPVFHYLVALAGRRIPVAPYACFGTPEFARSAAGSMGEGKAVLLAN
                     HGMVSVGRSLSEAFRIAENVEFAAMVYSRALALGEPRVLSEAEVDRVMDALKDYGSSV
                     IMPEPLKEEDQ"
     gene            3241..3948
                     /locus_tag="PK146_01765"
     CDS             3241..3948
                     /locus_tag="PK146_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPE90996.1"
                     /translation="MNFDLTLQLGEKIMGEGGSGYGVSDLTDIKDFPFPGLSYGVTIM
                     LALSPEIVSGIGDGPTRTYHDEYERVNGELARTGSETVRFLEGAGFRAVLLGPTSEQF
                     DESTLSVPFPHKTAAIRAGVGWIGKSDLLVSEEFGSAFRMCTVLTDAPLTPGTPSTES
                     LCGPCRACVEACPAKAPTGREWSAGTHRDELVDIFACYRQTRDFTKEMGWSHSICGIC
                     IQACPWTQKYISSRSCP"
     gene            complement(3926..5377)
                     /locus_tag="PK146_01770"
     CDS             complement(3926..5377)
                     /locus_tag="PK146_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPE90997.1"
                     /translation="MSPPMIIAGIVVWFTAGSLLSWHARKNLGEGMIEYFLADRKVGG
                     FISAMTYSATTYSAFMMVGLVGLTYSSGIGSLGFEMTYLAATVILMVIFAPRYWAAGR
                     IFRLVTPSELLARRYGSPMAGAVSAVLCLVMLVPYASVQLMGIGYLLEVLSGGAIPFT
                     VGMLIAAGVSFVFSWWAGLRSVALTDALQAVVMLVASLLLLGFITLALFPGGLPEALG
                     SRQDLLKVSWTSPMFLGLTLPWAFFAVTNPQVVQRLYIPSSVKNLRKMILGFSAFGFV
                     YTVLCVVFGLAAAVKIPGLEKADNAMAMLLSSVPSPLALVVSLSITAAAVSTMNSIIL
                     TLSSMFGRDVVRTFLPDISERTELGICRLLIPVITVVCFFFARMQLGLIAVLSSMASG
                     GLLMQLPTILGAFFWKKATAAGAVSSMIAGGLAVSFMYVQGLKPLGHWPPVWGILVSG
                     TVFIAVSLATRPPIDTEDFFSSLEGYIRDSFGS"
     gene            complement(5374..5580)
                     /locus_tag="PK146_01775"
     CDS             complement(5374..5580)
                     /locus_tag="PK146_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE90998.1"
                     /translation="MTQREKTWVLFSTLYGIVNVVVPFTLLRNVGTLAGAFLFWNLIT
                     AAVVLAGVWITSGWKDRGGEETLR"
     gene            complement(5606..6112)
                     /locus_tag="PK146_01780"
     CDS             complement(5606..6112)
                     /locus_tag="PK146_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HPE90999.1"
                     /translation="MDREKRLEKLSEILAGSEGPVSGASLADLFGISRQAVVQDIAIL
                     RDRGHRVLATARGYVLREGSRRRVFPVRHDAQEIAEELNTIIEAGGRVIDVVVDHPVY
                     GEIRGNLDLASREDVARFVNVLKSTGQSPLLSLSRGFHVHTVEAPDERTLDAIERALA
                     DKGFLEME"
     gene            6271..7053
                     /locus_tag="PK146_01785"
     CDS             6271..7053
                     /locus_tag="PK146_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HPE91000.1"
                     /translation="MSDMTPREVRQAIREGKWTGPTAGMAPEFVQANLVIVPSDWAFD
                     LLLFSQRNPRPCPLLEVTEPGDPEPRELAAGSDIRTDLPRYRVYRDGVLAEEPTDIRD
                     LWRDDLVSFLIGCSFTFEGALMDAGVPVRHVEMGVNVPMYITSVPCRPAGRLKGQVVM
                     SMRPIPAVMIARAVSTTALFPAVHGAPLHVGEPGMIGIEDLSRPDFGDPVEIREGEIP
                     VFWGCGVTPQAALMASKPPFAITHSPGHMFLTDRKDREYSVF"
     gene            complement(7078..7488)
                     /locus_tag="PK146_01790"
     CDS             complement(7078..7488)
                     /locus_tag="PK146_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91001.1"
                     /translation="MDDLERRVVILSLVARKGDNPDLWGYARLRKSILLLQIKEDVPL
                     GFVFGLERERPVSPELQLFLERMLYDNNLVSTETPSGFRRDLGSEGIRLLKEHPDIAE
                     RYSEQVDRVFERVRDMSLTDLRVAARNAYLSHPS"
     gene            complement(7582..8745)
                     /locus_tag="PK146_01795"
     CDS             complement(7582..8745)
                     /locus_tag="PK146_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPE91002.1"
                     /translation="MKKKVIVLGGGLVGSVMALDLAFDEDYAVTVADANRNVLDRLVA
                     RSGDRMTPLDNVDFSSPASITRAVKDHDIVVGAVPGSMGFAMMGAVIRAGKSMSDISF
                     MGEDYFRWDEEAKKAGVTLMEDVGVSPGFSNMLIGSSVSEMDSVESVEIYVTGLPTEP
                     KEPFNYRFVFSPDDCIEEYVRPVRLKRSGKIIEMPALSENMIYRFKIPGVELPEMEGF
                     LTDGLRSLLKTIPASNICEKTLRYPGTAERLAFLREIGLFSTGPVKVKGQKVVPRDVF
                     AALAYPMMKLGEKEKEFTFLQVDINGYLKGKPILHRYRMYDERDEKTGYTSMARTTGF
                     TCVIMARLMAEGLVDMPGVHAPEAIGDKPEVVARFIAEMEKRGVKVTKEVSGS"
     gene            9189..>10016
                     /locus_tag="PK146_01800"
     CDS             9189..>10016
                     /locus_tag="PK146_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPE91003.1"
                     /translation="MKAVQIGGGLVGQLIVADMMKDFEVTVVDLDRNTLEEIEKKHPG
                     VRTAVASATDASRLAPILEDADIVTAGVPGRFGYEMMKTVIGLGKNLVDISFMAEDFE
                     ELDGLAREKGVTVIPDMGVAPGMSNFLMGRGSALLDEVEGAYIYVGGIPTKAVPPFNY
                     QVTWSPKDCIEEFTRPVTIVRDFRKEVVEATSGLHLREFPGVGTLEGFYTDGLRSLAK
                     NIKARNLGEMTLRWPGHVEQMRLLRSMGLFEETPRNLGGREVVPLDVAADLLFPMWKM
                     "
BASE COUNT         2180 a         2800 c         2913 g         2123 t
ORIGIN      
        1 actacataaa cattgaggat tcggaggaga tcttgagggc catccggatg acatcatcgg
       61 acacccccat cgatctgatc gtccatactc ctggaggtct tttgctggct gcctcccaga
      121 tagcagcggc cctgaacaaa cacgaggcga aggttaccat ttttgttccg cactatgcca
      181 tgtcaggcgg tacccttatt gctcttgccg ctgacgagat cgtgatggac cgtcacgcgg
      241 tccttggccc ggtggatccc cagatcgggc agtatccggc cgtctctatc ctgaagaccg
      301 ttgaggataa gcctgtcgct gagctagacg atgagacact cattctggcc gacatatcac
      361 gaaaggcgat agaacagacc cgtaggttcg tggcaagcct tctcgttgac aacatacccc
      421 gtgaagaggc ggagaggttg gcgggacttc tcaccgaggg acgctggacg cacgattacc
      481 ccataagcag cgaggaggca gcagaactgg gtcttttcat atccacagat atccccgagg
      541 agatatgcca ggtcatgagg ttataccccc agtcaggcca gcgccgtccc tcggtccagt
      601 acgtccccat gccctaccat gggaccaccc ataacacggg tatcaaatga cctccggccc
      661 ggtccggata tatcgtttaa ggagtgatca tatgaaaagc ctttccagga aagtcctatt
      721 gctcgcagtt gctgtgacct ttgccattcc tgctctcgcc gtcgccgccg acaggggtcc
      781 cgtcatcacg gcgggggaag agaccgtcag cgagcaggag atgatccgcc tgataatgga
      841 ccagtccggg gcagacaaaa tgatggctcc cttcgtcctg gcgcagcttt ccctggagga
      901 cagggagagc tttgccgaac aggtgtccat ggccctgctt ctatcggagg ccgcgttgcg
      961 aaagggactc cagctggacc cttccgtctc ggtccagctg aggtggaacg caattaacat
     1021 ccttgcccag gcctatgtgg cctctgtctc ttcgaagtgg gacctgggaa gaccagccct
     1081 tgaagcctgg ttctcaagtc atgaagagca ttacaaggaa cccgaatcgg tgcgggtcag
     1141 gcacatcctg gtggcctccg aaaaagaggc ggtggacgtt ctgctgaagg tctacgccaa
     1201 ggaagctgac ttcggaaggg tcgccgccga gaacagcatc gatcccggga gtgctgggtc
     1261 cggtggggac cttggatggg tctacagggg acagaccgtc ccagagttcg atagtctcgc
     1321 cttctccctc gagccgggaa ggacaggagg tccggtggag acgaagttcg gctggcacgt
     1381 gcttcaggtt cttgagaaaa ggccggcaag gtcgttcacc cttgatgagg tgcttccacg
     1441 ggtaagagag gacatacagc agcactacct cgaactcgag gtccagcgcc tggtcgattc
     1501 actgggttcg gagattgacc gtgacatact ctccactctt ggaggattcc ctgcctaccg
     1561 gaaagaccag taagggcatt tcttcctgcc ggcggagcgg atattccgcc ggcaggaaga
     1621 tccctagagc agaagctctc ctctcaagac ggtcgttgcc cttccggtga tcttcactcg
     1681 atctcctgtt atggctacgg tcagttcgcc ccccctcttc gatgcctgga aagctcggaa
     1741 ccgggatctt cccgtgatcc gcgaccagta gggagcaagc acggtgtggg ccgatcctgt
     1801 cactggatct tcttctattc cctcccaggg accgaagtac cgtgacagga agtcaaaatc
     1861 tgaagaccgg gaggttatga tcactccctg aaaacccagc ccgcccctgt caatgcctag
     1921 catggccttg aagtcgggct gaagtccacg gacagcagcc tcatcagtca tccttaaaag
     1981 cagcatctca gcgctcttgg aaaaccttgt atcctcgacg gaactgatcc cgatggcatc
     2041 cagcagtcca tcagggatct cagccggttc agttccgttc agggggaaat caagggtgat
     2101 cccgtcctct cccagggcag ccgccagttt cccgctggcg ctctcgaagg tgatcctcgg
     2161 aaaagaagaa gctctttcat tgaagatcac ccatgaggct gcaagggtag cgtgaccgca
     2221 gaggggaacc tccacggagg gtgtgaacca cctgatcgag aaggcggcgc agcctttccc
     2281 ctccggctct tttgaaggca cgacgaaggc agtttcggag agattcatct ccatggctat
     2341 tcttttcatt gtcacctcgt cagggaactt gtccacaagg cagaccgcag cggggttccc
     2401 cctgaagggc tcgtccgtga acgcatccac ctggaacaca ggaatgatca tttcgctgcc
     2461 tgatcccttc atattcgaaa cctcccgtcc gtacgtaatg gtgccaccag tatacctgag
     2521 ctgtggaaac aggtaagcta gttaggttat gatcgtgagc ggggtgaggc cgatgatgct
     2581 ggagagtgcc cgttacgaga tagttcttta cggcagaaag ctgctggaga gcggactggt
     2641 cacaggaacg ggaggcaacc tgagcataag gtccggcaga tttgcggccc tgtcgccaag
     2701 ctgcgttgag tacggtctga tgaaacctga ggacgtggtc gttgttgaca tggatggaag
     2761 agtgcaggat gggcgtctga aaccgaccag cgagatgttc ctccatcttg ccctctacct
     2821 ttcaagaccg gatattactg cggtggttca tacccactcc ccctacgcct gcgttatggc
     2881 ctgcctgggt cgggagatcc cggtttttca ctatctcgtg gcccttgcgg ggaggaggat
     2941 ccccgtggcc ccctacgcct gtttcggcac accggaattt gcccggtccg cagcgggatc
     3001 gatgggggaa gggaaagcgg tcctccttgc gaaccatggg atggtctctg ttggaagaag
     3061 tctatctgaa gccttcagga tcgcggagaa cgtcgagttc gctgccatgg tctactccag
     3121 ggcactggca cttggagagc ctagggtgtt gagcgaggcg gaggtcgacc gggtcatgga
     3181 tgccctgaaa gattacggat cttcagtgat aatgccggag cccttaaagg aggaagatca
     3241 atgaacttcg acctgacgct gcagctgggt gagaagatca tgggggaggg cgggtcgggg
     3301 tatggggtct cggacctcac tgacatcaag gactttccct ttccagggct ttcctatggg
     3361 gtgaccatca tgcttgccct ttccccggag atagtatcgg ggataggaga tggccccact
     3421 cgcacctacc acgatgagta cgaaagggtt aacggggagc tggccaggac agggtctgag
     3481 acggtcaggt tccttgaagg cgccggtttc cgggcggtcc ttctgggacc gacgagtgag
     3541 cagttcgacg aaagcacact gagcgttccc ttcccccaca agacagctgc cattcgcgca
     3601 ggggtgggct ggataggcaa atctgatctg ctggtctctg aggagttcgg ctccgctttc
     3661 aggatgtgca ctgtactgac cgatgcccct ctgacccccg gaaccccctc tacggagtca
     3721 ctgtgcggcc cctgcagggc ctgcgttgag gcatgcccgg cgaaagcccc tacgggcagg
     3781 gagtggagtg ccggtacgca cagagacgag ctggtggaca tttttgcgtg ttaccgacag
     3841 acgagggatt tcacaaagga gatggggtgg tcccactcca tctgcgggat atgtatccag
     3901 gcctgtccct ggacccaaaa atatatcagc tcccgaagct gtccctgata tagccctcca
     3961 gggaagaaaa gaaatcttcg gtgtcgatcg ggggcctggt cgctagactg acagctatga
     4021 acactgtccc ggagacgagg atcccccaga ccggaggcca gtgacccagg ggttttagcc
     4081 cctggacgta catgaagctg accgcaaggc ctcctgctat catgcttgat acggctccgg
     4141 cggcggtcgc ctttttccag aaaaatgctc ccaggatcgt cggaagctgc atgagcagtc
     4201 ctcccgaggc catggaggag aggactgcga tcaggcccag ctgcatacgc gcgaaaaaga
     4261 aacaaaccac tgtgatgaca ggtatgagaa gcctgcatat ccccagttca gtcctctcgg
     4321 agatatcggg aaggaatgtc ctgacaacgt ccctcccgaa catggagctg agggtgagga
     4381 ttatcgagtt catggtagat actgcggcgg ccgttatgct gagggatacc accagggcca
     4441 ggggggaagg aactgaagac aaaagcatgg ccatggcgtt gtcggccttc tccagcccgg
     4501 gaattttgac ggcggctgcc agcccgaaga caacgcagag gacggtgtag acgaaaccga
     4561 aggctgaaaa accaaggatc atcttccgca ggttttttac gcttgagggg atgtaaagac
     4621 gctggaccac ctgtggattg gtcaccgcaa agaacgccca cggaagggtc agcccaagga
     4681 acatggggga ggtccacgag accttcagaa ggtcctgtct tgatccgagg gcttccggga
     4741 gaccccctgg gaagagcgcc aaggtgatga aacccaggag gaggagactg gcgacaagca
     4801 tcaccaccgc ctgtagagcg tctgtcagtg ccaccgatct cagaccggcc caccaggaga
     4861 agacgaagga tacaccggcc gcaatgagca tccccaccgt gaaggggatg gcgcctcccg
     4921 aaaggacctc cagcaggtat cctatcccca tgagctggac cgatgcgtaa gggacgagca
     4981 tgacaagaca gagaacggcc gagacggctc cggccattgg gcttccgtaa cgtcttgcaa
     5041 ggagctctga gggggtgacg agccggaaga tccttcccgc cgcccagtac ctgggcgcaa
     5101 agataaccat aaggatcacc gtcgcggcaa ggtaggtcat ctcgaaacca aggctgccga
     5161 tcccggagga gtaggtcaac cccaccagcc cgaccatcat gaaggcgctg taggtggtgg
     5221 cgctgtaggt catggctgaa atgaacccac cgaccttgcg gtcggccagg aagtattcga
     5281 tcatcccctc acccaggttc ttcctggcat gccacgatag cagggagcct gcggtgaacc
     5341 acaccacaat cccggcaatg atcatgggag ggctcatcgc agggtctcct cccctccccg
     5401 gtctttccac ccggaggtga tccatactcc ggcaaggact acggctgcgg taataaggtt
     5461 ccagaaaagg aaggccccgg caagggtgcc cacgttcctg agaagagtga agggaacgac
     5521 aacgtttacg atcccgtaga gtgttgagaa caggacccag gtcttctctc tttgcgtcaa
     5581 gttcgatcgc cccccgggaa gagtgtcatt ccatctcgag gaatcccttg tccgccagag
     5641 ctctctcaat agcgtccagg gtcctttcgt cgggggcttc cactgtgtgt acgtggaaac
     5701 cccttgaaag cgagagaagt ggggactgtc cggtgctctt gagcacgttg acgaaacgtg
     5761 ccacatcctc ccgggaggcg aggtccaggt tgccccttat ctcgccgtaa acggggtggt
     5821 ccacgaccac atcgatgacc ctcccaccgg cttcgattat ggtgttgagc tcttcggcta
     5881 tctcctgggc gtcatgacga acagggaaca ctcttctacg gctcccctcc ctcaggacat
     5941 aaccccttgc ggtggccaga acccggtgac cacggtccct caggatggcg atgtcctgga
     6001 ccactgcctg gcgactgatg ccgaaaaggt cagccagact cgccccgctt accggaccct
     6061 cactcccggc caggatctct gacagtttct ccagcctttt ctccctatcc ataggtcagt
     6121 ttgtttcctc ctaacttgct gctacttgca gatattagca tatatttcag gttcttccaa
     6181 taaccgtggc gtttcgatta ttattgaccc gtagaagagt actgcctgaa aagaagtcca
     6241 gtgggggtgg tcaggatggt ttcccaggat atgagcgata tgacgccacg agaggtccgg
     6301 caggctatca gggagggaaa atggacaggc cccaccgcag gtatggcccc ggagtttgtc
     6361 caggcgaacc tggtgatcgt tcctagcgac tgggcctttg atcttctcct tttctcccag
     6421 aggaatcccc gaccctgccc cctcctggag gttaccgagc cgggggatcc ggagcccagg
     6481 gagcttgctg caggatctga catcaggacc gatctcccaa ggtacagggt ctacagggac
     6541 ggggtccttg ccgaagaacc gacagatatc agggatcttt ggagggatga cctggtctcc
     6601 ttcctgatag gctgctcctt tacctttgag ggtgctctca tggatgctgg ggttccggtg
     6661 aggcacgtag agatgggggt caatgttccc atgtacataa cctctgtccc ctgtcggcca
     6721 gcgggacgtc tcaaagggca ggtggtgatg agcatgagac ccattcctgc agtgatgatc
     6781 gcacgggccg tttcgacgac cgccctgttc ccggcagtac acggtgcgcc gctccacgtc
     6841 ggtgagccgg gtatgatagg gatagaggac ctttcaaggc ccgattttgg tgatcccgtt
     6901 gagataaggg aaggcgagat acctgtcttc tggggttgcg gggtgacccc ccaggcggcc
     6961 cttatggctt ctaaacctcc cttcgcaata acccactctc ccgggcacat gttcctgacc
     7021 gacaggaaag accgggagta ctccgtcttc tagggttttg gcagagacca gggatggtca
     7081 ggaggggtgc gagaggtagg cgttcctggc tgctacgcga aggtcagtca gagacatgtc
     7141 cctgaccctt tcaaagaccc tgtcgacctg ttcactgtac ctttctgcga tatcggggtg
     7201 ttccttcaga agcctgatgc cctcgcttcc gaggtccctc ctgaagccgg acggtgtctc
     7261 tgttgagacc aggttgttgt cgtagagcat cctttccagg aagagctgca gttccgggga
     7321 aacgggtctc tccctctcca aaccgaagac aaaaccgagg gggacatcct ccttgatctg
     7381 gaggaggagg atagatttcc gaagccttgc gtaaccccac aggtcggggt tatcgccctt
     7441 tctggcaacg agactgagga tgactacccg tctctccaga tcatccatga gggaccccct
     7501 ccaagggaaa tggtctatgt tactttacca gagtggagag aaaaagcaag ggcggtaccg
     7561 gccagggacc gccctggtat ttcaagatcc agagacctct tttgttacct tgaccccgcg
     7621 cttttccatc tcggcaataa atctggctac tacctcgggt ttgtcgccga tggcttccgg
     7681 tgcgtgaact ccgggcatgt caacaaggcc ctcggccatc agccgggcca ttatgacgca
     7741 ggtgaatccc gtggtcctgg ccatggaagt gtagcccgtc ttttcgtccc tttcatcgta
     7801 catcctgtag cgatgaagaa tgggtttgcc tttcagatat ccgttgatat cgacctgcag
     7861 gaaggtgaac tccttttcct tctcaccaag cttcatcatc gggtaggcga gagcggcaaa
     7921 gacatccctg gggaccactt tctgcccttt aaccttcacc ggtccggtgg agaagaggcc
     7981 gatctccctg aggaaggcaa gcctttctgc cgtaccgggg tagcggaggg tcttttcaca
     8041 gatattggaa gcaggtatgg tcttcagaag ggatctcaga ccatcggtaa ggaatccctc
     8101 catctccggg agttctaccc ccgggatctt gaacctgtag atcatgttct ccgaaagggc
     8161 gggcatttcg attatcttcc cgctcctctt gagcctgacg gggcggacat actcctcgat
     8221 gcagtcgtcg ggagagaata cgaacctgta gttgaagggt tccttcggtt cagtgggcag
     8281 tcctgtgaca tagatctcca cgctttcgac gctgtccatc tcactgactg aacttccgat
     8341 aagcatgttc gagaatccgg gacttacccc cacatcttcc atgagggtca ctcctgcctt
     8401 tttggcctcc tcgtcccagc ggaaatagtc ctcgcccatg aagctgatgt ccgacatgct
     8461 ctttccagct cgaatgacgg cccccatcat ggcgaatccc attgaacccg ggacggcacc
     8521 cacaactata tcgtggtctt tcactgccct tgttatcgat gccgggctgg aaaagtcgac
     8581 gttgtccagc ggggtcatcc tatcccccga cctggcgacc agccgatcga ggacattcct
     8641 gttggcatct gcgacggtga ccgcataatc ctcatcgaag gcaaggtcca gggccattac
     8701 ggatccgacc aggccgccgc cgagcactat cacttttttc ttcataggca aacctcctct
     8761 gggatcggtg ctgaagcaaa tatgtatgca ataattatct cacctggcaa caggccggtc
     8821 aaatggtcaa gggaaaaagg aaggggcaag ggcgtgagat gttgacggga gacggaaagg
     8881 gcaggtgtgc tcacctgtct ggttttacca atgtagagtt ctcctctctc acttttctgc
     8941 gtcacccgtg gcgagaagat tccagggcca gatcctcaca tcgctccgct cctcaggatg
     9001 acaacaacag atcaagaacg cggacagttt atgcactcga tggtttcgac ctacgattca
     9061 catattacga tcaacgctgc agtacaacgc tacaaggaaa tttgacttcc cggatatcat
     9121 tatttatact attgattgca tacaaaaggg tttagcccca ttcaatacaa acagaaggag
     9181 ggatctggat gaaggctgtt cagatcggag gagggcttgt cggccagctg atagtggctg
     9241 acatgatgaa ggatttcgag gtgaccgtgg tcgatttgga ccggaacact ctcgaggaga
     9301 tcgagaaaaa gcacccaggg gtcaggaccg ctgtagcttc tgctaccgat gcctcccggc
     9361 tggcccccat actggaggac gcagacatag tcacggcggg ggtaccggga cgtttcggct
     9421 acgaaatgat gaagaccgtc atcgggctgg gcaagaacct ggtggacatc tccttcatgg
     9481 ccgaggattt cgaggagctc gacggtcttg ccagggagaa gggggtaacg gtgatcccgg
     9541 acatgggagt tgccccgggt atgtccaact tcctcatggg cagaggttcg gcgctcctcg
     9601 acgaggtgga gggcgcatat atctacgttg gaggaattcc caccaaggct gttcccccct
     9661 tcaactacca ggtaacctgg tcccccaagg actgtatcga ggagttcacc agacctgtca
     9721 ctatcgtcag ggacttccgg aaggaagtgg tcgaagccac ttccgggctt catctgcgcg
     9781 aattcccggg agtggggacc ctggaggggt tctacactga tggtctgcga agccttgcga
     9841 agaacatcaa ggcaaggaac ctgggggaga tgaccctcag gtggccaggt cacgttgagc
     9901 agatgaggct cctccggtcc atggggctct tcgaagagac acccaggaac cttggcggca
     9961 gggaggtcgt gcctcttgat gtggctgcgg accttctctt ccctatgtgg aagatg
//
LOCUS       DAOPKQ010000026         9975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_7105_length_9975_cov_4.906956, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000026 DAOPKQ010000000
VERSION     DAOPKQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7105_length_9975_cov_4.906956"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PK146_01805"
     CDS             <1..1360
                     /locus_tag="PK146_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE91004.1"
                     /translation="YLRARARNQFRTGARRYRWVVWGTMALAYIIVFFHRLAAGVVRE
                     DLAAAFGISGTAFGNLASMYFYAYMVMQIPVGMLADSLGARITVSAGIFLSGAGSLIF
                     GLAPSIGWAYMGRFIVGIGASTVFVCMLKMLSEWYSEKEFATMSGVTTLIGNFGGLIA
                     QTPLALMVGIFTWRMTFAGIGVLSFVITGVCLFLIRNRPTEMGFEPVSSGGPEKSVHA
                     SDLMPALRAALRSKGIWPATALGALFSGAQLTFTGAWGVPWLVDVYGLSRNEASAIVS
                     LVVIGAMTGGVMIGKFSDMVGARRWPLILVGSLNVAAWSVIVFWGSGMPPLVILKPTL
                     LLMGIFSMAMVLCIAVTKETNNPRYTGVALSVLNMGFFIGIAAYPPAMGAMIDLLSSY
                     SPAIQYRGALSLCLAGAVVGLALAFRVPETHCRNITISQGGACSHDQTPTTGPGNGHS
                     EQ"
     gene            complement(1345..2127)
                     /locus_tag="PK146_01810"
     CDS             complement(1345..2127)
                     /locus_tag="PK146_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HPE91005.1"
                     /translation="MRNPRIILAALLVILLMAGTAFAGRDHQFRGKVDGHTRGALLFK
                     AFAEGLNPESMDMILDEEPDETGRVRHLFLDLYGCELGGVRIERLEIEAFDTDFTDPC
                     SWESSGPDVEEMLFVNARATILEKDVNRAISDAVFGDEDGNWHNLFLDFRKNGVYASG
                     YYTVRLLFKLDILIELEGRFGIVGGKQVWLEDYTMKVNRVDLPEVLTDRAISQIQPII
                     DLNEFVFPLTLKTVIQEEDRVILESRNEPERFNGIRYLYCSE"
     gene            2255..3193
                     /locus_tag="PK146_01815"
     CDS             2255..3193
                     /locus_tag="PK146_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE91006.1"
                     /translation="MDERKRVLLADGAMFTIAIFWGSGFGVTSWLLGFFSPMWLLTAR
                     FMLSTAILLVLFRKRLRFLGRRDILLGCFLGFLLFLTFVAHIIGLVFTTAGKQSFIAS
                     SNVVMVPFLFALFYRRWPSKIATVGAFLTTAGLLVMAFTPGMTFNFGDFLSLVLAFGI
                     ALHVLAVGNLSRRMDPIALTVIQLASATVFLLASAIFFEPFPDVSAIAPAALLGLVYV
                     AVFVTVIPFLVQTVAQRFSPEVHAAILLSLEGPFGYVIAVMIGQEVLNSQVVLGGVII
                     LLGVILAECDTFLRRALHLPGADVHLPGSAEGGPDA"
     gene            3186..4343
                     /locus_tag="PK146_01820"
     CDS             3186..4343
                     /locus_tag="PK146_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE91007.1"
                     /translation="MHRVRKLLPRMAGSNFAIMSFSYVYFLLPLYLDHAGLKSQATIG
                     WILGAYYAAATLPRPFMALLVERFPFRVSLSGAAICCALGSTGLALSGVSLPLLILSR
                     ILMGFGFGLFLVSLTTYQTMVIPQEVRGGAFALITIGAMAPFLVVVPFLDWLLRHDGY
                     GIYVWIGPVVALACAMVGATFSRVDRDRAGKKEVWGTYPELFRLPGARVLFLAVFIYA
                     MTDAAIISIPTLAMSKGLLSSFFMVAFAVGAILIRVVGFRLMDIFPKDVIYTAMTALT
                     SLSLFGTSFAGSSASFAFWGFVYGIGVGVGFPLSLAMIGDVAPPELRPKATALVWFIL
                     DGCFFITPVILGYASSFLGVAGAFRAIPGMLFFAAPAVYFLLWRPIGKKGF"
     gene            4385..5515
                     /locus_tag="PK146_01825"
     CDS             4385..5515
                     /locus_tag="PK146_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPE91008.1"
                     /translation="MTNPLLGSISGLPMVSRPAAVEYSTLKNEIVAKVDSIMESRDDI
                     FSLIGGNQVSTMRVNHANHAGTMAVVFLYNRFDLLALAVPWVYRTYISHGFSTEYFPL
                     LFQTFITSIERTVSPGAAGEIMPVYLWLLENHETILRTRFDADEIGLTSSPEREAERV
                     AFRDALLTGDHGFCLRAAEKDLAERGGIRDLYVHILQPSLYDIGRMWERGEVSVAEEH
                     LASAVAGRVMSALYARIASSERTKRTCVVTAVPNEHHEIGARMVADLLEEDGWSVYYL
                     GSNIELSSAVSFIKERNPFLVAVSVALPFTLQGAAELISEVKSDPFLADVRFMAGGLA
                     FSGAGDLWKSIGADGYARDASEAVELARQWFSEGDRRRSGQI"
     gene            5502..6473
                     /locus_tag="PK146_01830"
     CDS             5502..6473
                     /locus_tag="PK146_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPE91009.1"
                     /translation="MDRSDPSVEKVIKKFFGSESRTILLTLDPQGLIVSSNGGMERIF
                     GLPGGFSMGRNIFDFILPENRDRLKGVFETLTKKSPPESPQCPFRKETLNFRHSSSAV
                     YTMACFFSRCGGTVVLIGETRRMSDSEIIIRMTDLNNELASLSRELTRKNVELERANA
                     TINRLLNTDHLTGIANRRALLEFLAEKIEKEDELPLSLVMADIDHFKRVNDSLGHSTG
                     DEVLSAFGKILSGQVRERDLCGRYGGEEFLIVLPRTEIHEAESVAERVRTTVEEAETG
                     DPPVRVTASFGVAVSIAGGSSDDLIGRADKAMYRAKALGRNRVVTQD"
     gene            complement(6460..6801)
                     /locus_tag="PK146_01835"
     CDS             complement(6460..6801)
                     /locus_tag="PK146_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91010.1"
                     /translation="MLKLEVEHYIQLGEKVRERAAELCRMYLKVYFDCEDCEVLVTNV
                     EVGHSGRFRIMFTESEMEDILYLPPEYFWLTDDEVALRMKIDREIREGQLEERRRLRT
                     CQEAKPPVNPA"
     gene            complement(7255..8439)
                     /locus_tag="PK146_01840"
     CDS             complement(7255..8439)
                     /locus_tag="PK146_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE91011.1"
                     /translation="MKMRLERPSFNIKLLILALTHFLNDIHGTFLPTFIPVIVSRLGI
                     SYAQAGLLKSLSGIIHMIVQPMAGYVSDLFSRPYAIIVGPLMTALGAALLPLGPTYGI
                     TFIIVGLWAFGSAVYHPLGHGSVGYVGNPGKLAFYLAVFSVGGILGSTLSPLYAIFLA
                     KVFGFGPLMPVAAMVPVSIGALLVIRYIPTLKAEGSEGTPSPMGFLRSFSKTFRVIFP
                     VWIVCVCRDTAVEGIRFFLPLFIASRGGSIVNIGTILFLINTVGTFSPMLGGPIADRF
                     GRRKVIEITMALSPFFLVPAALTSGFLSISLYMIGNALLQGILPVTGAAAQEMAPGAR
                     STAASMVTGLSFGLAGLLLAPIGALADRTSLTAVLIIIGALPLLPMPVFWMAWKQESR
                     NT"
     gene            8569..>9975
                     /locus_tag="PK146_01845"
     CDS             8569..>9975
                     /locus_tag="PK146_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE91012.1"
                     /translation="MENARQDILDLLMELVGIPSVSCTPAEAEAGRFIHSRLGELPYF
                     RDHPGNLQLLPVPGDPFGREVVMAFVEAVPTVKGTVVLTGHYDVVGAKDFGSLEDLAF
                     SPLEYTSALKERGLDGEAGEDLKSGDYLFGRGVMDMKCGLAVEMALLSEAAQEQSSLG
                     VNLLFLAVPDEEVGSAGMRAAVPALLKIKEDRGLDYLGAILTEPGNAGAPDAEGEVVF
                     LGAVGKVMPFFYCAGIGSHVGQYFEGLSASLLASHISLLVEGNPLMADRAGDETAPPP
                     ACLSIRDIRNSYSVTIPDRAAIFFNYMTLAKTPAEIMKEMKQVAEDACERTVGQIRES
                     ASRLGLSADVPRPRVVTFEEFASRTDLVRGGGAKAGVRELVRSMDPALDDRQRSLAVV
                     TEMLGWAPPEGPLVIVGFLPPYYPHRRNDGQNPGDLRVRRVAERVIELARRDHGIAMS
                     SREFFAGICDLSYMGFQGS"
BASE COUNT         2012 a         2743 c         2943 g         2277 t
ORIGIN      
        1 gtaccttcgc gcgcgggccc ggaaccagtt caggaccggc gcgcgaaggt accggtgggt
       61 cgtctggggc acaatggccc ttgcatacat aatagttttc ttccaccgcc tggccgccgg
      121 agtggtgcgg gaggatcttg ccgcagcctt cgggatatcg ggaacggcct tcggcaacct
      181 ggcctccatg tacttttacg cctacatggt catgcagatc ccggtcggta tgctggccga
      241 ctcactgggg gcaaggatca ccgtctcggc agggatcttt ctttccggtg ccgggtcatt
      301 gatcttcggt cttgcccctt ctatcgggtg ggcttacatg ggaaggttca tcgtgggcat
      361 aggtgcttct acggtctttg tctgcatgct gaagatgcta tccgaatggt acagcgagaa
      421 ggaatttgcc actatgtcag gggtgaccac cctgataggg aatttcggcg ggctcatagc
      481 tcagacaccc ctggctctga tggttggtat ttttacctgg aggatgacct tcgctgggat
      541 aggagtactg tccttcgtca taacgggagt atgcctgttt ttgataagga acaggccgac
      601 agagatgggc tttgagcccg tctcatccgg ggggcccgag aagtcggttc acgcttcaga
      661 tctcatgccg gccctgaggg ctgcgctgag atcgaagggg atatggcctg ccactgccct
      721 tggcgcactc ttttcaggcg cccagctcac ctttaccggt gcctgggggg ttccatggct
      781 ggttgatgtc tacggcctgt cgcggaatga agcctccgcg atcgtctcac tggtggttat
      841 cggggcgatg accggagggg tgatgatcgg gaagttctca gacatggtgg gggccaggag
      901 atggccactg atactggtgg gctctttaaa cgttgcagcg tggagcgtaa tagtcttctg
      961 gggttcagga atgcccccac tggtcatact gaagcccacc ctcctgttga tgggtatctt
     1021 ctctatggcg atggtccttt gtatagccgt tacaaaggaa acgaacaacc cccgctacac
     1081 gggggtcgca ctttcggtcc tcaatatggg ttttttcata ggcattgcgg cctacccgcc
     1141 agccatgggc gcgatgatcg acctcctgtc ctcatattcg ccagcgatac aatacagggg
     1201 cgctctttcc ctctgcctgg ccggggccgt tgtgggtctt gcccttgcct tcagggtccc
     1261 cgagacccac tgcaggaaca tcacaatttc acagggcggg gcctgtagcc acgatcaaac
     1321 ccctacgacc ggtcctggca atggtcactc cgagcagtag aggtaacgta taccgttaaa
     1381 tctttccggc tcgtttctgc tctcgaggat gaccctgtcc tcctcctgga tgacagtctt
     1441 cagggtaagg gggaacacga actcgttgag gtcgattatc ggctgtatct ggctgatggc
     1501 cctgtcggtg agtacttcgg gcagatcaac cctgttgacc ttcatggtgt agtcctcgag
     1561 ccagacctgc ttcccgccca caataccgaa acggccctcg agctcgatga ggatgtccag
     1621 cttgaaaagc agcctgacag tgtagtaccc actggcgtag actccgttct tgcggaagtc
     1681 gaggaagagg ttgtgccagt tgccgtcctc gtcgccgaag acggcgtcgg aaatggccct
     1741 gtttacgtct ttttcaagaa tggtggcccg ggcattgacg aagagcatct cctctacgtc
     1801 cggtccggag gactcccagc tgcatgggtc ggtgaagtcg gtgtcgaaag cctcgatctc
     1861 gagtcgttca attcttacgc cgcccagctc gcatccgtaa aggtcgagga acaggtggcg
     1921 tacgcgcccc gtctcgtcgg gctcctcgtc aaggatcata tccatcgact cggggttcag
     1981 accttctgca aaggccttga agagcaaagc gccccttgtg tggccgtcga cttttcccct
     2041 gaattgatgg tcccggccgg caaaggcagt tccagccatc agaaggataa caagcagggc
     2101 agcaagtatg attctcgggt tgcgcatcgt gagacctccg atcctgttcg ggggatgacc
     2161 acgctagaga atgatatcat accaaccttt gtacacaacc ggatttctgg tggtatgctg
     2221 tatacgtact attactgaag ggtcggtgct cgaattggac gagagaaaga gggtccttct
     2281 tgccgatgga gcaatgttca ccatcgccat attctggggg tcgggctttg gcgtgaccag
     2341 ctggctcctg ggtttcttct cgcccatgtg gcttcttaca gcccggttca tgctgagtac
     2401 ggccatactg ctggtactgt tccgtaagcg cctcaggttc ctggggagaa gggacatcct
     2461 tctcggctgc ttcctcggct tcctcctctt cctgactttc gtggcccata taatcggcct
     2521 ggttttcacg accgccggga agcagtcctt catagcttcc tcaaatgtgg tcatggttcc
     2581 cttcctcttt gccctcttct acaggagatg gccctcaaaa atagccacag tcggggcttt
     2641 ccttacgacg gcaggactgc tggtcatggc cttcactccg gggatgacct tcaacttcgg
     2701 tgatttcctg tcccttgtcc tggccttcgg tattgccctg cacgtccttg ccgtgggcaa
     2761 cctgagccgg aggatggatc ccattgccct gacggtcatc cagctggcct cggcaacggt
     2821 ctttctcctt gccagcgcaa ttttcttcga gcccttcccc gatgtcagtg ccatcgctcc
     2881 cgcggcgttg ctggggcttg tctatgtagc cgtattcgtt acggtcatcc ccttcctggt
     2941 ccagaccgtt gcccagaggt tcagccccga ggtccatgcc gccattcttc tctcccttga
     3001 aggccccttc ggctatgtta ttgccgtcat gatcggccag gaggtcctga actcccaggt
     3061 agtcctcggg ggggtgatca tccttctcgg ggttattctt gctgagtgcg ataccttcct
     3121 caggagagcc cttcacctcc ccggggccga tgttcatctg ccgggctctg cggagggcgg
     3181 acccgatgca tagggtcagg aaactgcttc caaggatggc aggaagcaac ttcgcaatca
     3241 tgagcttcag ctacgtctac tttctgctgc ccctatacct ggatcacgcc gggctgaagt
     3301 cccaggcaac gataggctgg atcctggggg cctactacgc cgctgcgacc ctccctcgtc
     3361 ctttcatggc tctgctggtg gaacgcttcc ccttcagggt cagcctgtcg ggggccgcta
     3421 tctgttgcgc ccttgggagc accggactgg ctctctccgg ggtctccctt ccgcttctga
     3481 tcctctcaag gatactcatg ggttttggtt tcgggctatt cctcgtgtcc ctgaccacct
     3541 accagaccat ggtgatcccc caggaagtcc ggggaggggc cttcgccctt ataaccattg
     3601 gcgccatggc gcctttcctg gtggtggtgc ctttcctgga ctggctgctt cgtcatgacg
     3661 gctacggtat ctatgtctgg ataggtcctg tggttgccct ggcatgcgcc atggtgggag
     3721 cgaccttctc cagggtggac agggacaggg ccgggaaaaa ggaggtgtgg ggtacctacc
     3781 cggaactttt ccgtctccct ggagcgagag tgctctttct tgccgttttt atctatgcca
     3841 tgaccgatgc tgcaataatc tcgatcccga cgctggccat gagcaagggg ctcctctcat
     3901 cgttcttcat ggtggctttc gcagtggggg ccattctgat aagggtcgtc ggtttcaggc
     3961 tcatggacat atttcccaag gatgtcatct ataccgcaat gactgcgttg acctcccttt
     4021 cgctcttcgg cacgtccttt gcgggttctt cagcgagttt cgctttctgg ggtttcgtct
     4081 acggcatcgg ggtgggtgtt ggattccccc tctcgctggc catgataggg gacgttgctc
     4141 ctcctgaact gcgcccgaag gccacagccc ttgtctggtt catcctcgac ggctgtttct
     4201 tcataacccc cgttatcctg gggtatgctt cttccttcct gggggtagcc ggggcgttcc
     4261 gtgcgatacc cggtatgctc ttcttcgcgg ctccggctgt ctactttctt ctctggaggc
     4321 ccatcggtaa aaaagggttt taatgatatc attttaaaac attaccgaaa ggagaggacg
     4381 gcagttgacc aatccccttc tgggttccat ttcaggactg cccatggtct cccggccggc
     4441 ggcggtggaa tactcgaccc tgaagaacga gatagtggca aaggtcgatt ccatcatgga
     4501 atcgagggat gatatattct ccctgatagg cgggaaccag gtgagcacca tgcgggtcaa
     4561 tcatgcgaac cacgccggga caatggccgt tgttttcctg tacaacaggt tcgatcttct
     4621 cgctcttgcc gtgccctggg tatacaggac ctacatttcc cacggcttct ccacggagta
     4681 ctttcccttg ctcttccaga cgttcatcac atccatagag aggacggtgt ccccgggggc
     4741 ggccggcgag ataatgccgg tctacctttg gctgctggaa aaccatgaaa ctatcctcag
     4801 gacaaggttc gatgctgacg agatcgggtt aacatcaagc ccggagagag aggccgagag
     4861 agtggccttc agggatgccc tccttaccgg cgatcacggt ttttgcctca gggcggcaga
     4921 gaaggatctc gctgaacgag gggggatccg tgatctctat gtccatatcc tccagccatc
     4981 tctatacgat ataggaagga tgtgggagag aggcgaggta tcagttgccg aggagcacct
     5041 tgcctcggca gtggcgggaa gggtcatgtc cgcgctctat gccaggatag cctcctcgga
     5101 gaggaccaag aggacctgcg tggtcactgc cgtgccgaat gaacatcacg agataggcgc
     5161 aaggatggtg gcagacctcc tggaggagga cggctggagc gtgtactacc ttgggagcaa
     5221 catagaactg tcaagtgccg tgtctttcat aaaggaacgg aatcccttcc tggtcgctgt
     5281 atctgttgcc ttgcccttta ccctgcaggg tgcggcggaa ctgatctcgg aggtaaagag
     5341 cgatcccttt ctggctgatg tgaggttcat ggcaggaggc cttgccttct ccggggccgg
     5401 tgatctctgg aagtccatcg gggccgatgg atatgcaagg gatgcctcgg aggcagttga
     5461 gttggcgcgt cagtggttca gcgaggggga caggaggaga agtggacaga tctgaccctt
     5521 cagtcgaaaa ggtgatcaag aagtttttcg gatccgagag caggaccata ctgttgaccc
     5581 tcgacccgca ggggctgatc gtcagctcca acggggggat ggagaggatc ttcgggcttc
     5641 ccggaggctt ctccatggga aggaacatat tcgacttcat cctgcctgag aacagggacc
     5701 gtttgaaagg agttttcgaa actctcacaa aaaaatcgcc ccctgaaagt cctcaatgcc
     5761 cgttccggaa ggagaccctc aacttccgcc actcctcttc ggcagtctac accatggcct
     5821 gtttcttttc ccgctgtgga gggaccgttg tcctgatcgg agagaccagg agaatgtccg
     5881 acagcgagat cattatcagg atgacggacc tcaacaacga gctagcctcc ctttcgaggg
     5941 agctcaccag gaagaacgtg gagcttgaga gggccaacgc gacaattaac cggcttctta
     6001 acaccgacca cttgacagga atcgccaaca ggagggccct gcttgagttt ctcgcggaaa
     6061 agatcgagaa ggaggacgag ttgccccttt ccctggtaat ggctgatata gatcacttca
     6121 agagggtcaa cgacagtctg ggacacagta ctggcgacga ggtcctctca gctttcggaa
     6181 agatcctctc ggggcaggtc agggagaggg atctctgtgg aaggtacggg ggggaggagt
     6241 tccttatcgt ccttccccgc acggaaatcc atgaagccga atccgtcgcg gagagggtgc
     6301 gaacgaccgt ggaagaggcc gagacaggcg atcctccagt cagggtgaca gcaagcttcg
     6361 gggtggccgt ttccattgca gggggaagtt cagacgacct catcggaagg gccgacaagg
     6421 ccatgtacag ggccaaggct ttgggacgga acagggtggt cacgcaggat tgacgggcgg
     6481 cttcgcctcc tggcaagtcc gtaaccttct tcgttcttcc agctgacctt cccggatctc
     6541 ccggtcgatc ttcatcctga gggctacctc gtcgtcggtg agccagaagt attcaggggg
     6601 gaggtagagg atatcctcca tctccgattc ggtgaacatg atacggaacc ttccgctgtg
     6661 ccctacctcg acgttggtta caagcacctc gcaatcctcg cagtcaaagt agaccttcag
     6721 gtacattctg cacagttcgg cagcccgctc cctcaccttt tctccgagct ggatatagtg
     6781 ttcgacctca agcttcagca ccttccccgc cccccttgat cgctctcagg agggagtata
     6841 acatactatc ctagtaaacc gctaccttgg ggttggagat atgataaaaa aaggaggcag
     6901 acatcaggaa gggcaaaagc ttaactcgta acttgcagga tcaagagtga aacagtgaac
     6961 ggaaaatgca agggtttgtc attccgagga gggaaacgac gaggaaccac gacctggttc
     7021 ctttagatcc cagatcttca ccacagacaa ggtcgtatgc cgtaacggct gcatatctat
     7081 tcccctcgtt aaagcacctt cctcgttaat tcacctcggg accttgtcgg ggctatgccg
     7141 gggccgggtc cttcaggcaa gctgaaaggt aggcttcgct ctgaccaggt tcgagcactg
     7201 ccaacgcagg ttaggacgca gataaaaaat tgattttaat tttgtccttt cgagtcacgt
     7261 gttacgactt tcctgcttcc aagccatcca gaatactggc atagggagaa gggggagggc
     7321 cccgataatt attaggactg cggtgagact ggtgcggtcc gccaacgctc caatgggggc
     7381 gagaagaaga ccagccagtc cgaaggagag tcccgtgacc atggatgcgg cagtactcct
     7441 agcccccggg gccatctcct gggcggcggc cccggtgacg ggaaggatcc cctggagaag
     7501 ggcattccct atcatgtaga gggagatcga caggaacccg ctggtaaggg ctgctgggac
     7561 caggaagaaa ggcgagaggg ccatggtgat ctcaatgact tttctgcgcc cgaacctgtc
     7621 ggcgatgggg cctccaagca tgggtgaaaa agtcccgacg gtgttgatca gaaaaaggat
     7681 agtgccgata ttgacaatgc ttcccccccg ggaggctatg aagaggggga gaaagaaccg
     7741 gatcccctcg actgcggtat ccctgcagac gcaaacgatc cagacaggga atatcaccct
     7801 gaaggtcttg ctgaaggatc ttaaaaatcc catgggggag ggtgtgccct cggaaccctc
     7861 agccttgagc gtagggatgt aacggatcac cagcaaggcc cctatggaga cagggaccat
     7921 agctgcgact ggcatcagtg gtccgaaccc gaaaaccttt gccaaaaaaa tcgcgtaaag
     7981 tgggctgagg gtcgatccga ggatccctcc cacggagaat acggcaaggt aaaatgccag
     8041 cttcccagga ttaccgacgt aacccacgct cccgtgtccc agggggtggt atacggcgct
     8101 cccgaaggcc cagagtccca ctatgatgaa agtgatcccg taggtcggtc ccaggggcaa
     8161 gagggcagct ccaagggcgg tcataagtgg tcctacgatt atcgcatagg ggcgtgaaaa
     8221 gaggtccgaa acataacctg ccatgggctg aacgatcatg tggatgatcc ctgacaggga
     8281 tttcagaagc cctgcctggg cataggagat ccccaggcgg ctgactatca ctggtatgaa
     8341 ggtaggtaga aaggtcccat ggatatcgtt aagaaaatgg gtcaacgcca gtataaggag
     8401 cttgatattg aaagagggtc gttccaggcg cattttcatg atcgggactt cctttcagga
     8461 gcatgactga cggctcctat tgtatcactg catacatgca tatcaccttc cttcagggtg
     8521 gtattattga gctgactttt tcctcttcga tgggagcgag gttcggcaat ggagaacgca
     8581 aggcaggaca ttcttgatct tctcatggaa ctggtcggga tacccagtgt ctcctgtaca
     8641 cccgccgagg cagaggcggg cagattcatt cactccaggc tcggagaact tccctacttc
     8701 cgtgaccatc ccgggaacct tcagcttctt cccgtcccgg gagatccctt tggcagggag
     8761 gtggtcatgg cctttgtgga ggcagtcccg accgtcaagg gtacggtagt cctcacgggg
     8821 cactacgacg tggtaggtgc caaagacttc ggatctctgg aggaccttgc cttctccccc
     8881 ctggaataca cttccgctct gaaggagaga ggtcttgacg gggaggccgg agaggatctc
     8941 aagtcgggcg actatctctt cggccgaggg gtcatggata tgaagtgcgg tcttgccgtt
     9001 gaaatggcgc tgctctccga agcggcgcag gagcagtcca gccttggggt caaccttctc
     9061 ttcctggccg tccccgacga agaagtgggt tcagccggga tgagggcggc tgttcccgcc
     9121 ctgttgaaga tcaaagagga taggggtctc gactatctcg gggccatact tacagagcct
     9181 ggcaacgcgg gcgcacccga tgccgaagga gaagtggtct tcctgggtgc ggtgggaaag
     9241 gtaatgcctt tcttctactg tgccggtata gggtctcacg tagggcagta ctttgaaggc
     9301 ctgagcgcct ccctccttgc gtcccatatc tcccttcttg tcgagggcaa ccccctgatg
     9361 gcagacaggg ccggggatga gacggcacct cccccggcat gcctctcgat cagggatatc
     9421 cgtaacagct attccgtaac gatccccgac agggcggcga tcttcttcaa ctacatgacc
     9481 ctggcaaaga cgccggcgga gatcatgaag gagatgaaac aggtcgccga agacgcctgt
     9541 gaaagaacgg tgggacagat cagggaaagc gcttccaggc tgggtctttc cgctgatgtt
     9601 ccccgccccc gggtggtgac cttcgaggag tttgcatcca ggactgacct ggttcgggga
     9661 ggcggagcaa aggccggggt aagggaactg gtcagatcca tggacccggc cctggacgat
     9721 cgtcagcgtt cactggcggt cgtcacggag atgctggggt gggctccccc ggaggggccg
     9781 ttggtgattg tgggcttcct ccccccctat taccctcacc gacggaacga cggacaaaac
     9841 ccgggcgacc tgagggtgcg gagagtggct gaacgcgtca tagagttggc acgcagggat
     9901 cacggcatcg ccatgtcatc cagagagttc tttgccggta tatgcgacct cagctacatg
     9961 ggcttccagg ggagt
//
LOCUS       DAOPKQ010000027         9897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_7171_length_9897_cov_4.234810, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000027 DAOPKQ010000000
VERSION     DAOPKQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7171_length_9897_cov_4.234810"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="PK146_01850"
     CDS             complement(<1..1227)
                     /locus_tag="PK146_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPE91013.1"
                     /translation="MSGFVTRTLSRNEAIMDLSRRMGFPWPVAVREKVPLWDALGLAL
                     GEDVTATDPSPPYDRSLRDGFAVRSFDLVGAGKTSPVFLTVCGEVPMGSMPDLKVGQE
                     QAVLVHTGGAIPAGADSVVMLEDTSLVGDLLEVRRSVQKSENLLLQGEEYASGRRLIR
                     KGDILDLRNIAAVASCGAVTVPVCDLRVAIMSTGDEIVPADSRHILPGMIRDTNSSII
                     MANMTRAGIRGVSLGIVGDDPDKLRAAFNAALESFDILVMSGGSSVSTRDHSVMLLRE
                     LGGGEVPVRGLNISPGKPTIVSGDPSRRKMAVCLPGHPHSCAVISSTFLVPLISSLIM
                     GEPLDRFRKVFLPAGEDVIGRSGVEEFVPVRIGHGGEAIPLWARSRYLLAMGGSDGLI
                     RLPENMETLRRGASVEV"
     gene            complement(1266..1868)
                     /locus_tag="PK146_01855"
     CDS             complement(1266..1868)
                     /locus_tag="PK146_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPE91014.1"
                     /translation="MSFRKNRKFKEKPAVFAFGHIMLPLVAVLALGLLVLGVRLLFVS
                     PESQVSYPETPEYPGAETETPPVVAKTTGETQVVAVPVPDGDGPSSEPKANPATPPAE
                     DNGPAAKPAEETRPAEPTVASRGSWIVQIGAFTLKDSAETLAREARNKNYNAFVVQAV
                     VGGKTYYRVRIPAGDQKVEADSLARELASKGYPTLVTRQE"
     gene            complement(1908..3434)
                     /locus_tag="PK146_01860"
     CDS             complement(1908..3434)
                     /locus_tag="PK146_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE91015.1"
                     /translation="MKDLSVKARARDWRYLREQEIELDALPSGGHPLWAMLFPAGYSV
                     GMASLGYQVIIAEMRALGVGVERFFPGPFPGLSVESGRPISDFPLISASVAYEPDIRT
                     ILSVFREWGLSSSRKVRFDLDQPVFGIGGALTYINPLIFSEMADYVVLGDGERVLDHL
                     VREARLFMKNGDRRRFLEGLAEHRSIYVPSLHDPMILKGHKVHREKNIIGDLDSVRGK
                     SQWIAPSSVFGKTALVELQRGCRRKCRYCTIPASFGRIRTRSAKRILEDLSPLVSVEG
                     VQAGLVSPETGDYPELSELLSGVASLGIPVSFASLRVDNINEEVVSALTRSGRHSLTV
                     APESGNDRLRRECGKYFSNDDIIERLFMASSMGVRQVKLYFMTGIPGETASDVDDIAG
                     LCDRVRSEARLRVTASVGVFIPKPMTYWEREEMIGERDASSRLKRLKKLFSEGPAKGC
                     RINIQQPKEAALEFSIAWRGLSAGDDPLLPAERRSGAPGPSPEQRSLLLEQLGILGYN
                     "
     gene            complement(3459..4628)
                     /gene="ftsZ"
                     /locus_tag="PK146_01865"
     CDS             complement(3459..4628)
                     /gene="ftsZ"
                     /locus_tag="PK146_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPE91016.1"
                     /translation="MAELFEIEGSQKQFHEVIKVIGVGGGGGNALNHIVRSGVTGVEF
                     IAANTDIAHLSLSEADLKIVLGRELTKGLGAGADPEIGMRSAEESVEEVREVLSGADM
                     IFLTAGMGGGTGTGASPVIAQIARENGALVVAVATTPFLFEGQRRRTQALEGIENLRT
                     NVDALIVIPNDRLLEITQKGTTLSDAFKLADDVLRQAVQGVTDLILRPGLVNVDFADV
                     RTIMKDAGTAIMGIGEAKGDDRASKAAHGAINSPLMDGPITGARGVLFNVTGGPNLGI
                     HEIQEAAQVVTEAADPQATILWGHVLDPEMDERVQITVIATGFNSGQCPRRTIPTARR
                     RGLSFTETEALAGKDALRQAAARHDPSGEVPENMEDLEDLDTPPVVRRRKFGHKP"
     gene            complement(4654..6006)
                     /gene="ftsA"
                     /locus_tag="PK146_01870"
     CDS             complement(4654..6006)
                     /gene="ftsA"
                     /locus_tag="PK146_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPE91017.1"
                     /translation="MRRGKEEPIIRSGLDIGTRKISLIVTETDPLSSEMQVIAVGSAR
                     ARGMSRGDIQDPDLVVESIRRAVDEAETAFDLNIRDTFLAVGPSSASVFRLEHRFPFR
                     EEGTPERPVTGKDMKDAVKGAVRAARERGEELLVHAIPLGYSVDSGSPVRDPRGLKGS
                     ELSVEVLFIGLPEDEVKRSITCAEKAGLKVLGVVHKSIASAYGSLSEEEICHGAVVVD
                     IGAGTSTVTCVRDGAVRDVTVSPVGGDLVTQDVAKLLMIPPSKAEYLKREISLFEDPC
                     DLMDELEFELDGEPFVATVQDVLDIVFPRMEEILFDFLSPRIEEFGQEKGLRSIVFSG
                     GVAGAMGFLDLINDNYEIPARIGGPVNAMSLPPPARGCEFASTVGIVNYLLEREAHPE
                     NLLEPCLVELTGAPDEEQARDILILEPRRTGPGRNIKRGKGPMGGFIDALKNAFRELF
                     "
     gene            complement(6031..6438)
                     /locus_tag="PK146_01875"
     CDS             complement(6031..6438)
                     /locus_tag="PK146_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91018.1"
                     /translation="MNNIISQQSAREGPVVVWGAGLPDPIPSEIDIESSVKDSVVPIH
                     ELESWKENLESSGFYRRVSSITISRREGRRIIEILERTGEGSVRVLLGEPPGDWPPLL
                     EAVDDILSQTGTKDRHLLIDTTYSGKILVRVIS"
     gene            complement(6454..6813)
                     /locus_tag="PK146_01880"
     CDS             complement(6454..6813)
                     /locus_tag="PK146_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91019.1"
                     /translation="MRKSLVRFLALLLFMSGVLYCTEERSQFLRLRSVSFDSLALVPD
                     DHFWRRIDPECHRYWPLLSLRARSIERSIMMEAPVEIRITHAGFGSFAVESTPLEPWF
                     MVFWAGGEWFLTREGRM"
     gene            complement(6794..7723)
                     /gene="murB"
                     /locus_tag="PK146_01885"
     CDS             complement(6794..7723)
                     /gene="murB"
                     /locus_tag="PK146_01885"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPE91020.1"
                     /translation="MRWRSRLDCENLPFCSSGTRLKDLTTLGVGGPAECLARPVTRRD
                     LTRILDLCREEEAPTWFIGGGSNVLIPDEGLPGVTIQTGGLNGVLWEEQDDSVVLETE
                     SGVLLASLLALSMNKGWEGLEFSVGIPGTVGGALHGNAGVEGRSLGDLVESVLLVDDK
                     GSLAWKRRQDIRFSYRFTDLLAGNRAIVACKLVMKKGDPAKISSSVRGFINRRKGQPR
                     GIRTAGCIFKNPCGESAGKLLDETGCKGLSVGDAVVSSIHANFIENKGSASSKDIFRL
                     ISNCREKVFQRTGILLEMEICLLGEIAHAEVAG"
     gene            complement(7701..9146)
                     /gene="murC"
                     /locus_tag="PK146_01890"
     CDS             complement(7701..9146)
                     /gene="murC"
                     /locus_tag="PK146_01890"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPE91021.1"
                     /translation="MNDFDGRFENLERVHLMGIGGAGMSGLALLLRQIGMKISGCDVS
                     HTYYINKISPNGIEFALGHDKEHLDRFKPDAIVFSSAIPSETEELVEAARRGIRIFKR
                     AEVLSWLFNLRKGIGVAGTHGKTTTASMIGLILENAGLDPSVAIGGELCDIGGNAKLG
                     QGAHMVAELDESDGSFELFRSHVAIVTNADWDHVDYYPSFSSVLKAYERFLSNREPGG
                     AAIICGEDKGLSALLQNRINGRHLTYGWGARWDWGAQDVSHNRGGGVTYTLSRNGTPA
                     CEIFLQVSGEHNVLNSLAACAAADTIGIPLEVAAQALRTFKGAKRRLQHMGSIVSLEV
                     DIFDDYGHHPREIAATLGTLKKMFPERRVMAVFQPHRFTRTAAMYRDFAEVLSMADGV
                     FLLPVFPADEMPIEGVSSTLIGDILKQKGHKGYDFCSDMDEVVTRICSRVREGDVIAT
                     IGAGDVSIVGEKIQQRLERKGVTLDAVAIKA"
     gene            complement(9143..>9897)
                     /locus_tag="PK146_01895"
     CDS             complement(9143..>9897)
                     /locus_tag="PK146_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPE91022.1"
                     /translation="FVSKMKGVPILLHEPNARAGRVTCIAAKARIPVCTGWDVCEPLP
                     SGGFTPVGVPVRNLRRLPPEEAWKLLGYDLDLPPSPRVLVLGGSLGSDPMTVLFSKVS
                     AERLFRSRNFLLVGSSERAVRTSRNLWLLPREWEIDLLYSIADGVVSRAGASTLGELV
                     VFGLPSVIIPWRGAADDHQAHNARVFSEKGFGSIWEIDNGIHENLAFMIDYELKRARL
                     GQAFQFPGRGTGEACEKIWELLQQIAEGRGRN"
BASE COUNT         2116 a         3007 c         2547 g         2227 t
ORIGIN      
        1 tacctctaca gaagctcctc tccttagtgt ttccatgttc tcgggaagcc ttatcagccc
       61 gtctgatccg cccatggcca gaaggtaccg cgacctggcc cacaacggaa tagcttcgcc
      121 tccgtgtcct atccttaccg ggacgaactc ctccacaccg ctcctcccga taacgtcctc
      181 tccggcgggc aggaagacct ttctgaaccg gtccagaggt tcacccatta taagggatga
      241 tatcagcggc accaggaagg tcgaggaaat aaccgcacat gaatgggggt ggcctggcag
      301 gcagacggcc atttttctcc ttgagggatc gcctgagaca atggtaggct tgcccggtga
      361 aatattgagc cctcttacag ggacttcccc gcctccaagt tcacggagga gcatcacgga
      421 atggtccctg gtgcttaccg aagaaccgcc gctcattacc aggatgtcaa aactctccag
      481 agcagcattg aaagcagctc tgagcttatc cggatcatcc cccacaattc ccagggaaac
      541 ccccctgatc ccggcccttg tcatgttcgc cataatgatg gaagagttgg tgtcccgtat
      601 catccccggc aagatgtgcc ggctgtcagc agggactatc tcgtctccgg tgctcatgat
      661 cgccaccctg agatcgcaga ccggaaccgt taccgctccg caggatgcga cggctgcaat
      721 gttccggagg tccagtatat ctcccttgcg gatcagcctg cgcccactgg cgtactcctc
      781 tccctgaaga aggagattct cgctcttctg gaccgacctg cggacctcaa gcagatcccc
      841 aacaagagag gtgtcctcca gcataaccac cgaatcagct ccagccggga tggcacctcc
      901 tgtatgcacc agaacggcct gttcctgtcc gaccttcaga tcaggcatgc tccccatggg
      961 tacttccccg caaaccgtca gaaagaccgg ggaggtcttt cccgccccga caaggtcaaa
     1021 tgatcttacg gcgaacccgt cacggaggct tctgtcatag ggcgggctcg ggtccgtggc
     1081 agtcacatcc tcaccaaggg caagaccgag ggcatcccag agaggcacct tttcccttac
     1141 ggccacgggc caggggaagc ccattctccg ggagaggtcc attatcgcct cgttccttga
     1201 gagcgttctt gtaacgaacc ctgacattct atctcctcgg gaggggaaca ggcataatcg
     1261 gctccctact cctgccgtgt caccagcgtt ggataccctt ttgaggccag ctctctcgca
     1321 aggctgtccg cctcgacctt ctggtcgcct gccgggatcc ttaccctgta ataggtcttt
     1381 ccgccaacta ccgcctggac gacaaaggcg ttgtagttct tgttcctggc ctctcttgct
     1441 agggtctcgg cagagtcctt gagggtaaaa gccccgatct gaacgatcca gcttcccctg
     1501 gaagcgactg tcggctctgc gggtctcgtc tcttctgcag gtttggcggc gggcccgtta
     1561 tcctcggcgg ggggtgttgc tgggttggct ttcggttctg aggagggacc atcaccgtcg
     1621 ggaacgggga ccgccacgac ctgggtctct cccgtggtct tagcgacaac gggaggggtt
     1681 tctgtttcag ccccggggta ttccggggtt tccggataac tcacctggct ttcgggggat
     1741 acgaaaagaa gcctgacccc gaggacaaga agtcccaggg ccaggacagc aacaagaggc
     1801 agcattatat ggccgaaggc aaagacagcc ggtttctcct tgaacttcct gttcttcctg
     1861 aagctcactg gaaatcacct cacaaagaga gtgttcttcg atggccttta attgtaacca
     1921 agaataccaa gttgttccag cagaagggat ctctgttcgg gagacggtcc aggagcgccg
     1981 gacctccgct ctgccggcag caggggatca tcccccgcgg acagccccct ccaggcgatc
     2041 gagaactcaa gggctgcttc tttcggttgc tggatattta tcctgcaacc ctttgctggt
     2101 ccttcgctga agagtttctt caatctcttc aacctggagg aggcatccct ttcacctatc
     2161 atttcctctc tttcccaata ggtcatcggc ttcggtatga aaacccctac ggaagctgtg
     2221 accctgagcc tcgcttcgga gcggacccgg tcgcagagac cggcaatatc atcgacatcg
     2281 gatgctgtct cacccgggat acccgtcatg aaatagagtt tgacctgtct gacccccatg
     2341 ctggatgcca tgaaaagacg ctctatgatg tcgtcgttgg agaaatactt accgcactcc
     2401 cttctcagcc ggtcgttccc tgactcggga gccactgtta gagaatgtct cccgctcctt
     2461 gtcagggcac ttacaacctc ctcgttgata ttatctaccc tgagggaagc aaatgagacc
     2521 gggatcccga gggatgccac accggaaaga agctcggaaa gctccgggta gtcccctgtc
     2581 tcgggtgaca ccaggccagc ctgaactcct tccacggaga ccagggggga aaggtcctca
     2641 aggatccttt tggccgaacg cgtcctgatc cttccgaaag aggccgggat ggtacagtat
     2701 ctgcacttcc tcctgcaccc tctctgcaac tcgaccagtg ctgtcttccc gaaaaccgag
     2761 gagggcgcta tccattggct cttgcccctt acgctgtcaa ggtccccgat aatgttcttt
     2821 tccctgtgga ccttgtgccc cttaagtatc attgggtcat ggagggaggg cacatatatc
     2881 gaccggtgtt cggcaagacc ctccaggaat cttctgcggt caccattttt catgaaaagc
     2941 cgtgcttccc ttaccaggtg gtcaaggacc ctttccccgt cacccaggac cacgtaatcg
     3001 gccatctcgg agaatatcag ggggttgata taggtcaggg cgcctccgat gccgaagaca
     3061 ggctgatcaa ggtcgaacct cactttcctg gatgacgata gcccccattc ccggaagacc
     3121 gaaaggatgg tccggatatc gggctcgtaa gcaacagaag cggaaataag agggaaatcg
     3181 gaaatgggtc ttccggactc caccgacagg ccgggaaagg gcccgggaaa aaagcgctcc
     3241 accccgacac caagggcccg catctctgct atgatgacct ggtatccgag gcttgccatc
     3301 ccgacgctgt accctgccgg aaagagcatg gcccacaaag ggtgtccacc tgaaggaagg
     3361 gcgtccagtt cgatctcctg ttcccggagg tatctccagt cccttgcccg ggctttcact
     3421 gaaaggtcct tcaacggcgc aggtcccgac cgtaccgatt atggtttatg accgaatttc
     3481 ctccttctga cgacgggagg ggtatccagg tcttcgaggt cttccatgtt ctccggaact
     3541 tctccggatg gatcatgtct tgccgcggcc tgcctgagag cgtccttccc cgcaagggct
     3601 tccgtctcgg tgaacgagag acctcttctt cttgcggtcg ggatcgtcct tctggggcac
     3661 tgccccgaat tgaagcccgt tgctataact gttatctgca ccctctcgtc catctccgga
     3721 tcaagaacat gaccccacag tatggtggcc tggggatcgg ctgcttcagt gactacctgg
     3781 gcggcctcct ggatctcatg gatacccaga ttcggcccgc cggtgacgtt gaagagcaca
     3841 ccccgggctc cggtgatagg tccgtccatt aatgggctgt ttatagcccc atgggcagcc
     3901 ttggaagccc tgtcgtcccc cttggcttct cctatgccca ttattgcggt gccagcatcc
     3961 ttcattatgg tccggacatc ggcaaaatca acgttcacca gccccggacg aaggataagg
     4021 tctgtcacgc cctggacggc ctgcctcagg acatcgtcgg cgagcttgaa ggcgtccgag
     4081 agagttgttc ctttctgggt gatctcaagt agtcggtcgt taggaatgac tatcagggcg
     4141 tcaacgttgg tgcgaaggtt ctcaatcccc tcaagggcct gggtcctcct tctctggccc
     4201 tcgaaaagga atggtgttgt cgcaacggcg accaccaggg ctccgttctc cctggcgatc
     4261 tgggctatga cgggagaggc ccctgtcccg gtcccccctc ccattcctgc ggtcaggaag
     4321 atcatatcgg ccccgctcag gacctcccgg acctcttcaa cggactcctc ggctgacctc
     4381 attccgatct cgggatcggc tcccgccccc aggcctttgg taagttctct ccccagaacg
     4441 atcttcagat cggcctcgga gagactcaga tgagcaatat cggtatttgc ggcgatgaac
     4501 tctacgccgg tcactccgct ccttacaata tggttaaggg cgttaccccc tccaccaccg
     4561 acaccaataa ccttgataac ttcgtgaaac tgtttctgcg atccttcgat ctcaaagagc
     4621 tcagccatga gagcaacctc cacgaaaaac ggattagaac agttccctga acgcgttctt
     4681 cagggcatca ataaatccgc ccattggtcc cttgcctctt ttaatatttc ttcccggacc
     4741 tgttcttctt ggttcaagga tcaggatatc ccttgcctgc tcctcatcag gagcaccggt
     4801 caattcgaca aggcacggct ccaggagatt ttcagggtgg gcctcccttt ccaggagata
     4861 gttcacgatc cccacagtgg aagcaaattc acaacctctt gccggtgggg ggaggctcat
     4921 tgcgttaaca ggtccgccta tcctggcggg tatctcgtaa ttgtcgttga tgagatccaa
     4981 aaaccccatg gcgcccgcaa ccccgcctga gaagacgata ctgcggagcc ccttctcctg
     5041 gccgaactct tcgatccttg gggacagaaa atcgaaaagg atctcctcca tcctcggaaa
     5101 gacaatgtcc agaacgtcct ggacggttgc cacaaatggt tctccgtcca gctcgaattc
     5161 gagctcgtcc atgaggtcac agggatcttc aaagagggat atttcgcgtt tcaggtactc
     5221 cgcctttgaa ggaggtatca tgagcaattt cgcaacatcc tgtgtgacga gatcccctcc
     5281 cacgggcgag accgtaacat ccctgaccgc cccatccctg acacaggtca cggtcgaagt
     5341 accggcacct atgtcaacta ccactgcccc atggcagatc tcctcttcag atagggaccc
     5401 gtaggctgaa gcgatagatt tgtggacgac accaaggacc ttcagtccgg ccttttcagc
     5461 gcaggtgatc gatcttttca cttcatcttc cggaagtcct ataaagagga cctctacaga
     5521 cagctcggag cccttcagac ccctggggtc cctgacagga gagcccgaat cgacggaata
     5581 gccaagggga atggcgtgga cgagcagctc ctcgcccctt tcccttgcag cccttacggc
     5641 gcccttgacg gcgtccttca tgtctttgcc tgttaccggc ctttcgggag tgccttcctc
     5701 ccggaagggg aacctgtgtt ccagcctgaa gaccgaagcc gaggaggggc caacggcaag
     5761 gaatgtatcc ctgatgttaa ggtcgaaggc agtttccgcc tcatccactg ctcttctgat
     5821 ggattcgacc accaggtcgg ggtcctggat atcacccctg gacattcctc tcgctcttgc
     5881 ggaaccgact gcaattacct gcatttccga agagaggggg tccgtctcgg tcactatcag
     5941 cgagatcttc ctggtgccga tatcaagccc cgatctgatg atgggctctt ccttccctct
     6001 tctcatgggc aatcctccag ggtattggga tcaggaaatg acccttacca ggatctttcc
     6061 cgaataggtc gtatcgatca gcaagtgccg gtcctttgta cccgtttgcg aaagtatatc
     6121 atccactgct tccaggaggg ggggccaatc accggggggt tctcccaaaa ggacccttac
     6181 acttccctct ccggtccttt ccaggatctc gatgatcctt cttccctctc tcctgctaat
     6241 ggtaatggaa gagacccttc gataaaagcc tgaactttca agattttcct tccaggattc
     6301 cagttcgtga atgggtacca cagaatcctt gacagagctc tctatgtcaa tttccgaagg
     6361 gatagggtca ggaagacccg ctccccagac gaccaccggt ccctccctgg cggactgctg
     6421 ggatattatg ttgttcattg agtgatggac cgactacatc ctgccctccc tggtaaggaa
     6481 ccactcgcct cctgcccaga agaccatgaa ccatggttca agtggagtcg attcgactgc
     6541 gaaggatccg aaacccgcat gagtgatccg gatctcaacg ggtgcctcca tcatgatcga
     6601 ccgttcgatg ctccgtgctc ttaatgaaag gagaggccag taacgatggc actccgggtc
     6661 gatccttctc cagaaatgat catcggggac aagggcgagg gaatcgaacg agaccgacct
     6721 caggcgcagg aactgggatc gctcctccgt acagtatagc acccctgaca tgaagagaag
     6781 caaggccagg aacctaacca gcgacttccg catgggcgat ctcacccaaa aggcatatct
     6841 ccatctccag aaggatcccg gttctctgaa aaaccttttc cctgcagttg gagatcagcc
     6901 tgaaaatatc cttcgaactg gctgaaccct tgttctcaat gaaattggca tgaattgaag
     6961 agacaacagc atctcccaca ctgagccctt tgcatccggt ctcatccaga agtttcccgg
     7021 cgctttcgcc gcaggggttc ttgaaaatgc aaccagctgt cctgatcccc cgaggctgcc
     7081 cctttctcct gttaatgaac cccctgacgg aagaagagat ctttgccgga tcacccttct
     7141 tcatgacaag tttgcaggcg acaatggccc tgttgcctgc aagaaggtct gtgaacctgt
     7201 atgagaaacg gatatcctgc ctcctcttcc atgccaggga gcctttatcg tcgaccagca
     7261 ggaccgattc cacaagatcg ccgagagatc tcccctctac cccggcatta ccgtgaagag
     7321 ctcctccgac cgttccgggg atcccgacgg agaattccag gccttcccaa cccttgttca
     7381 tcgacagagc gagcagtgag gcaagaagaa cacccgactc cgtctccagc accactgaat
     7441 catcctgctc ttcccaaagg acgccgttta gaccgcctgt ctgtatagtc actccaggaa
     7501 ggccttcatc ggggatcagt acattgctgc ccccaccgat gaaccatgta ggggcctctt
     7561 cctccctgca aaggtcaagg atccttgtaa gatcacgacg cgtgaccggc cttgcaagac
     7621 attctgccgg cccgcccaca ccaagggtcg tgaggtcttt cagacgtgtg cctgatgaac
     7681 agaacggaag gttctcacag tcaagccttg atcgccaccg catcgagggt aacgcctttc
     7741 ctttcaaggc gctgctggat cttttcgccc acaattgaca catcccccgc accgatggtg
     7801 gcgatcacgt cgccctccct gaccctggaa catattctgg tcaccacctc gtccatatcg
     7861 ctgcagaagt cgtaaccctt gtgacctttc tgcttgagga tgtcgccaat aagggtggag
     7921 gatacaccct ctatgggcat ttcgtcggca gggaagaccg gcagaaggaa tactccgtca
     7981 gccatggaga gcacctccgc aaagtccctg tacatggcag cggtccttgt gaacctgtgg
     8041 ggctggaata ccgccatgac ccttctttcg gggaacatct tcttcagggt ccccagggtt
     8101 gcggcgatct ccctcgggtg atggccgtaa tcgtcgaata tgtcaacttc aagcgaaacg
     8161 atggagccca tgtgctgcag gcgcctcttg gcccccttga aggtcctcag ggcctgggcg
     8221 gccacctcga ggggaattcc gatagtatcg gcagccgcac aggccgcaag agagttaaga
     8281 acgttgtgct ctcccgagac ctggaggaag atctcacagg ctggtgttcc gttccgggag
     8341 agggtgtagg tgacccctcc acccctgttg tggctgacat cctgcgcgcc ccagtcccat
     8401 ctcgcacccc agccatatgt gagatgtctg ccgttgatcc tgttctggag aagcgccgaa
     8461 aggcccttgt cctctccgca tatgatagcg gcacctccag gttccctgtt ggagaggaac
     8521 ctttcgtagg ccttcaggac agagctgaaa cttgggtagt aatctacatg gtcccaatct
     8581 gcgttggtaa cgatggcaac atgggagcga aagagttcga aggagccgtc gctttcgtca
     8641 agctcggcta ccatatgggc accctgtccc agtttggcgt tccctccgat atcacagagc
     8701 tctcctccga tggcgacaga tgggtcaagg ccggcattct ccaggatgag cccgatcatg
     8761 gaggccgtgg tggtctttcc atgggttccg gcaacaccga taccctttcg caagttgaag
     8821 agccagctta acacttccgc tcttttgaag atccggatcc ctctcctggc tgcctcgacc
     8881 agctcttctg tctctgaggg tatggcactg ctgaaaacta ttgcatccgg tttgaacctg
     8941 tccaggtgct ccttgtcatg gccgagggcg aattcgatcc cgttgggaga gatcttgttt
     9001 atgtaatagg tatgtgaaac atcacatccg cttatcttca ttccgatctg acgtaaaaga
     9061 agagccagac cgctcatccc ggctcctcca attcccatca gatgtaccct ctcgaggttt
     9121 tcgaatcggc catcgaagtc gttcaattgc gacctctccc ttcggcgatt tgttgtaaaa
     9181 gctcccagat cttctcacag gcttcaccgg ttcccctacc aggaaactga aaggcttgac
     9241 cgagccgagc cctctttagt tcatagtcta tcatgaacgc aaggttttca tgaatcccgt
     9301 tatcgatctc ccatatggac ccgaaccctt tttcagaaaa aaccctggca ttatgcgcct
     9361 ggtgatcgtc tgccgcgccc ctccagggga ttatgacaga agggagacca aatacaacca
     9421 gttcccccag ggtggaagca cccgctcttg acactacccc atcggcgatg gaatagagaa
     9481 ggtcgatctc ccactcccgt ggaagaagcc agaggttcct cgatgtccgg acggctcttt
     9541 cgctggaacc aaccaatagg aaatttctgc tgcggaaaag cctctcagcg gaaaccttgc
     9601 tgaacagaac ggtcatggga tcgctcccga gagacccccc aaggacaagt acccgggggc
     9661 tggggggaag atccagatca taccccagaa gcttccaggc ttcttcgggt ggcaatcgtc
     9721 tcaggtttct aaccggaacg cccacagggg tgaaccctcc ggaaggaagg ggttcgcaaa
     9781 catcccagcc cgtacagacg ggaatcctcg ctttagccgc aatacaggtt acccttccgg
     9841 ccctggcatt gggttcgtga agaaggatag gaacaccctt cattttagag acgaaca
//
LOCUS       DAOPKQ010000028         9887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_7179_length_9887_cov_4.712266, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000028 DAOPKQ010000000
VERSION     DAOPKQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7179_length_9887_cov_4.712266"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PK146_01900"
     CDS             complement(<1..994)
                     /locus_tag="PK146_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91023.1"
                     /translation="MISLWETSRISFRALTANKMRSALTMLGIIIGVMAVIMMFAIGS
                     GANREISDRFSSMGSNMLTVRPGASQGPGGIRSGTVNTLTVDDARAISEECMAVAYAA
                     PVYGGSAQVVYGNQNWSTSVTGTTPEYLFVQDWTVGSGIVFTEQDVRSATKVSVIGKT
                     IVDNLFGGEDPIGKTIRIRNVPVVVVGVLGEIGTNPMGRDQDDIILVPVTTAQRRLFS
                     SSRSGSVSTIMVKARSSELLNAAQDQVESLLRMRHRVRKGEEDDFSVRNMAQMVENAK
                     AATRTMTMLLTAVAGVSLLVGGIGIMNIMLVSVTERTREIGIRMALGAKTIDIRL"
     gene            complement(991..1686)
                     /locus_tag="PK146_01905"
     CDS             complement(991..1686)
                     /locus_tag="PK146_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91024.1"
                     /translation="MNGIVRVESITKIYSLGDVKVEALRKVSFSVEAGEFLSIMGASG
                     SGKSTMMNILGCLDTPTGGQYFLEGHNVSRLSGDELADIRKHKIGFVFQGFNLLPRMT
                     ALENVKLPMLYNGVHPSMRDRKAMDALEMVGLGDRANHQPQQLSGGQQQRVAIARALV
                     NDAPIILADEPTGNLDSRTSKDIMDLFTDLNREYGRTIIQVTHEAEMASYGKRVITFR
                     DGMVLKDERTERP"
     gene            complement(1707..2876)
                     /locus_tag="PK146_01910"
     CDS             complement(1707..2876)
                     /locus_tag="PK146_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE91025.1"
                     /translation="MKKNRKFLAAFLVFVAAAGIWYYRSRSSDVPVVQTTTVVRGGIV
                     KTVSATGSLNAVNTVNVGAQVSGTIRKIHVNYNSLVQKGDLLAEIDPSLLEANIRQSE
                     ASVLSALASLQEARANLAEAKNNLERNRELFSRGYIAAMELDQSQTAYETALSRTRSS
                     EASLSHARADLDYRKINLGHTRIVSPIDGVIINREVDEGQTVNASQSAPTLFTIAEDL
                     SRMQVEANIDEADIGLIRQGQPVQFTVDAFPDESFVGSVKEVRLAAATTDNVVTYTVI
                     ISVENEKKILMPGMTANVSVIVEKKDNILKVASSALRFRPSADSGGSQFRGTALWTLE
                     KGELVPHLVKTGIYDGMFTEVEGEGLVEGMEVVTYISSASEGRSSSAFGFGGRPR"
     gene            complement(2873..4171)
                     /locus_tag="PK146_01915"
     CDS             complement(2873..4171)
                     /locus_tag="PK146_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE91026.1"
                     /translation="MLTMKRFLQSICFLILFSLGAAAYASGAPPGSIVLTLEDCINTA
                     ISGHPDLAAGRSDLEASEARVGQAQSSRYPSLGFSTGFSERSSGGTGPSEGSWSNGIT
                     LSQLVTDWGRTHATVRRSLLDVDSRTLALEAKQSDVVFEVTRAYFQLLRSVKDLEVAE
                     ETLALNEVRLEQAEAFFKVGKVSKYDVTAAQVSKSNADLALIRARTSRKEAMTALKAA
                     MGQAGAPDFRVVDVEGHETDVRDDDVPSLDEAIGIALENRPDLLAYQVALRSAETSVT
                     LARLDNVPQLHLSGNYGWGNSGFWGGDSWRAGLTLSFSLYDGGLQREKTREAMAGLDG
                     AEARLEAYRQIVVSDVTQAVLAASDASEAVSAASEGLRMATENLEIATGRYRVGVGSP
                     LEVSDATRNYTEAKAAWYAALYDRLTARAALKKAMGVTAR"
     gene            complement(4276..6441)
                     /locus_tag="PK146_01920"
     CDS             complement(4276..6441)
                     /locus_tag="PK146_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPE91027.1"
                     /translation="MADSSHASIIAAELGISEEKVLDTGALLQEGCSVPFIARYRKEA
                     TGSLDEVAVLAIRDRFAQLEELDRRKAAVIRSLRERDLLTEELERAVAVAPNMTALED
                     IYLPFRPKRRTRASIALGKGLKGLADALVDAAAGERTDLDPLSEAARYIDGGKGVKSA
                     DDALAGARDIIAERASEDRRVRVALRAVFARNGRLSSKVVRGREEEGLKYRDYFDLEE
                     PARSTPSHRILAVFRGEREGFLTVSARPPSELAFLEMERVFIKAEGRAADEVRKAIRD
                     GYVRLAGPSMETELKNALKVRADREAIGVFSSNAREVLMAPPLGAFPVMAIDPGIRTG
                     CKLVILDARGRLVLDDVIFPFSGRKAEEEARRKVCRILEKHPVKAVAVGNGTAGRETM
                     AFLQGMELPGNPRAILVNESGASIYSASKTAREEFPDRDVTVRGSVSIGRRLQDPLAE
                     LVKIDPKSIGVGQYQHDVDQKKLTQSLEDVVVSCVNAVGVELNTASPHLLSYVSGLGP
                     GLAANIVAWRGSMGPFRSRAQLREVSRLGPRAFEQSAGFLRIRGSVNPLDASAVHPDH
                     YVIVERMASDLGCSVEDLLENESMRERINVTHYVCEKAGIPTLRDIMSELAKPGRDPR
                     KNFESVEFSPSVHGIEDLNPGMELTGVVSNVTAFGVFVDVGVHQDGLVHVSQMSDRFV
                     KKPSDLVKAGQKVRVSVLEVDLQRRRISLAMKGVRQDPS"
     gene            complement(6451..6993)
                     /locus_tag="PK146_01925"
     CDS             complement(6451..6993)
                     /locus_tag="PK146_01925"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HPE91028.1"
                     /translation="MKISPFPEVDSILRQAGLCDCLWVAPSSMTVSQWVRMKCMFGCP
                     DYGKNASCPPHVPSVQECRELLGEYSHAVLIHLVCDVHSEERGSWCASYQKLLVEAER
                     QVFLAGHEKAFLLSFDNCNLCGECTADPRDCRLPYMRRPTMEAFAIDVYSSVRRLGYS
                     ISVKKDREEKPDRFALLLVE"
     gene            complement(6990..7496)
                     /locus_tag="PK146_01930"
     CDS             complement(6990..7496)
                     /locus_tag="PK146_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPE91029.1"
                     /translation="MIRLKTSEGDILLEMFEEKAPKTVENFLNYVKTGHYDGTIFHRV
                     IDGFMIQGGGFDETMSQKATEEPVENEASNGLRNEAYTIAMARTQDPHSATTQFFINV
                     KDNHFLNFTSPDVRGWGYTVFGKVIEGHDVVDRIRSVETCSRAGLDDVPVEPVTIIKA
                     IVEKEEER"
     gene            7653..8126
                     /locus_tag="PK146_01935"
     CDS             7653..8126
                     /locus_tag="PK146_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPE91030.1"
                     /translation="MTRGEKGFTLTELLITILIIGILSGSLFAVYGDARSKAVAARIV
                     ADLMTLRSASAVFFSERGRWPSEIDYGDLLNYLGRDPVGEGLNERAIAYGIISPDWSP
                     SVFISARVGNGNGVAPGTRKILEKEASSLGLLDEEGSPYSSGSNIVMVILKKNEP"
     gene            complement(8219..8536)
                     /locus_tag="PK146_01940"
     CDS             complement(8219..8536)
                     /locus_tag="PK146_01940"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HPE91031.1"
                     /translation="MRRSAVAIVLLSLVIFMVFCDAGAGEAPEDTLFTYDFSGLVFEV
                     RFIEGAVILYLPDGPLTLPQGISASGARYTDGSTTFWIKGDGAHLEINGSLVGKKTDV
                     PQQ"
     gene            8628..9056
                     /locus_tag="PK146_01945"
     CDS             8628..9056
                     /locus_tag="PK146_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPE91032.1"
                     /translation="MPKVIAVCAGKDRQKPKTEMEAAHFVKGKGINGDGHFGMGERQV
                     SLLRSEDISEAENSAGFPFPPGSLAENLVIAGLPEDLSPGQTMKIGESVILIVVEKGK
                     KPGEPHSYDYRGWCLLPEKGYFLRVEKGGKVQPGDPVILA"
     gene            9233..9823
                     /locus_tag="PK146_01950"
     CDS             9233..9823
                     /locus_tag="PK146_01950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPE91033.1"
                     /translation="MTDNSSKEKFEQRDFEKPGFLFRFCLSVIHPLISGPRLYSPFVE
                     GLELEGSERVLEFGCGNGVLTVYLAKALDKGGTVVGVDTSSYMTERAMTRLKGHSNAR
                     ILKGDIRSLGLEPESFDLVTFIHVLHDIAPGKRQSTMDTLSCLLAPGGRLCLMEPVSS
                     FHGIPEEKIRSIANDAGLKVTETVPMGRRVKISCVK"
BASE COUNT         2096 a         2923 c         2584 g         2284 t
ORIGIN      
        1 gaagccttat atctattgtc ttcgccccga gagccattct gatgcctatc tcccgggtcc
       61 tctcggtgac ggataccagc atgatgttca ttatccctat cccgcccacg agaagggaga
      121 ctcccgccac tgccgtgaga agcatggtca tcgttcttgt tgcagccttg gcgttctcca
      181 ccatctgtgc catgttcctt acggagaaat cgtcctcttc ccctttacgg acgcggtgtc
      241 tcattctcag gagcgactct acctggtcct gagccgcatt gagcagttca gagcttcggg
      301 cctttaccat aatggttgag accgacccgg acctggaaga actgaaaagc cttctctggg
      361 ctgtcgtcac aggcacaagg attatatcgt cctgatccct gcccatgggg ttggtcccta
      421 tctctcccag gaccccaacc acaaccaccg ggacgttcct tatcctgatg gtcttcccta
      481 tggggtcctc ccccccgaag aggttatcca ctatcgtctt tccgataacg ctgaccttgg
      541 tcgcgctcct gacgtcctgc tcggtgaata cgatacccga tccaacggtc cagtcctgga
      601 cgaagaggta ttctggcgtg gtacctgtga ctgatgtgga ccaattctgg ttgccgtaga
      661 cgacctgggc gctgcccccg tagacggggg cagcgtatgc cacggccatg cactcctccg
      721 atatggccct ggcatcatcc accgtaaggg tgttcaccgt cccgctacgg atccctccgg
      781 ggccctggct tgccccgggt ctcactgtaa gcatgttgct ccccatgctg gaaaacctgt
      841 cggagatctc cctgtttgcg ccggagccga tggcgaacat catgatgacc gccataacac
      901 cgattattat cccgagcatg gtcagtgcag agcgcatctt gttggccgtc agggccctga
      961 aggatatcct tgacgtttcc caaagcgaga tcatggacgt tccgtccttt cgtccttcag
     1021 caccattccg tcccggaaag tgatgaccct cttgccgtag gaggccattt ctgcctcgtg
     1081 tgtgacctgt attatggtcc ttccgtactc cctgttaaga tccgtgaaaa gatccatgat
     1141 gtccttgctg gtcctgctgt ccaggttccc cgtcggctca tcggccagga tgatcggggc
     1201 gtcgttaaca agggcacgtg ctatggccac tctctgctgc tgcccccccg agagctgctg
     1261 gggttggtgg ttagccctgt cacccaatcc caccatttca agggcatcca tggccttcct
     1321 gtccctcatg gaggggtgta ctccgttgta gagcatggga agctttacat tctccagggc
     1381 cgtcatccgg gggagaaggt tgaaaccctg gaagacaaaa ccgatcttgt gcttgcgaat
     1441 gtccgcaagt tcgtcccccg acaggcgact gacgttatgc ccttcaagga aatactgtcc
     1501 cccagtcggc gtatcaaggc accccaggat gttcatcatt gtcgactttc ctgaacctga
     1561 agctcccatg atggagagga attctccggc ctctaccgag aaactgacct tccgcaaggc
     1621 ttcaaccttg acatcgccca ggctgtatat cttggtgatg ctttcaaccc tgactattcc
     1681 gttcatcgtc atcttccgtc accccgtcac ctgggccggc ctccgaaccc gaaagcgctg
     1741 gatgaccttc cttccgaggc gctggatata taggtgacaa cctccatgcc ctcgaccagc
     1801 ccctccccct caacttcggt gaacattccg tcgtagatcc ccgtcttgac aagatgggga
     1861 accagttcac ccttctcaag ggtccagagg gccgtccccc tgaactgtga tccgccggaa
     1921 tccgctgaag gccgaaatct cagggcggaa ctggcgactt tcagtatgtt atccttcttc
     1981 tccactatta ctgatacgtt ggcggtcatg cccggcatca ggatcttctt ctcgttctcc
     2041 acagaaatga tgaccgtgta ggtcaccacg ttgtcggttg ttgcggccgc aagtcggacc
     2101 tccttgacgg aacccacgaa agactcatca gggaaagcgt caacggtgaa ctgtacaggt
     2161 tgaccctgcc tgatcaggcc aatatcggcc tcgtcaatgt tggcttcgac ctgcatcctg
     2221 gacagatcct cggcgattgt gaagagcgtc ggggccgatt ggcttgcgtt gaccgtctgc
     2281 ccttcatcga cctcccggtt aatgatcacc ccgtcgatgg gggatactat cctggtgtgc
     2341 cccaggttga tcttcctgta atcgaggtct gccctggcat gggaaaggga tgcttcagag
     2401 cttctggttc gggaaagtgc tgtctcgtag gctgtctgtg actgatccag ctccatcgcc
     2461 gctatataac ccctggagaa aagctctctg ttccgttcca ggttattttt tgcctcggcc
     2521 aggtttgccc ttgcctcctg gagggaggcc agggcggaga gtacgctggc ctctgactgc
     2581 ctgatgttgg cctccagaag agaggggtct atctctgcaa gaagatcccc tttctggacc
     2641 agggagttgt aattcacgtg tatcttcctg atcgttcccg atacctgggc tccaacgttc
     2701 acagtgttga ccgcattcag ggatcccgtc gcagaaacgg tcttgacgat acctccccga
     2761 acgacggtgg tggtctggac aacgggcacg tcagaagaac gtgaccggta gtaccagata
     2821 ccggccgctg ccacaaaaac aagaaaagct gccaggaact tcctgttctt cttcatcttg
     2881 ctgtcactcc catggccttc ttcagggccg cccgggccgt aagtcgatcg tataaagccg
     2941 cataccaggc agctttggcc tcggtgtaat ttctggtggc atcagagacc tccagtggtg
     3001 atcctacgcc taccctgtac ctcccggttg cgatctcaag gttctctgtt gccatccgca
     3061 gcccctctga ggctgccgag acggcctcag aagcgtctga ggctgccaga acagcctggg
     3121 taacgtcgct gaccacgatc tgcctgtaag cctccagtct ggcctcggcc ccatccaggc
     3181 cggccatggc ttctcttgtc ttctctctct gcagtcctcc atcatagagg gaaaaagaaa
     3241 gggtcagccc cgccctccag ctgtcaccgc cccagaaacc ggaattcccc cacccgtagt
     3301 tccctgaaag atgaagctgg gggacgttat ccagtctggc aagggtaacg gaggtctcgg
     3361 cggaccgaag ggctacctgg taagccagga ggtcgggcct gttctcaagg gcgatgccta
     3421 tcgcctcatc aagggaggga acatcgtcgt ccctgacgtc tgtttcgtga ccttccacat
     3481 cgacgaccct gaagtcaggg gctcccgcct ggcccatagc tgccttcaga gcggtcatgg
     3541 cttccttcct ggaggtgcgt gcacgtatca gcgcgaggtc tgcattgctt ttggaaacct
     3601 gggcggcagt cacatcgtac tttgaaacct tcccgacctt gaaaaaggct tctgcctgct
     3661 ccagcctgac ctcgttcaac gccagggtct cctcggcgac ctccaggtcc ttcacggatc
     3721 taagaagctg gaaataggct ctcgtgacct cgaacaccac gtcagactgc ttcgcctcaa
     3781 gggccagggt tctgctgtca acatccagca ggctccttct gaccgtcgcg tgcgtcctgc
     3841 cccagtcggt gaccagctgt gacagcgtga taccgttcga ccaggaacct tccgacggtc
     3901 cagtcccacc ggaggacctt tcagaaaaac ccgtggaaaa tcccagggaa gggtaacgac
     3961 tcgactgggc ctgcccaact ctggcctcgg aggcttcgag atccgacctc cctgctgcca
     4021 ggtcgggatg acctgagatc gccgtattga tgcaatcctc cagggtgagg acgatcgaac
     4081 ccggcggtgc acctgaagcg tatgcggcag cgccgagaga gaacaggatc agaaagcaga
     4141 ttgactggag gaaccttttc atcgtcagca ttggacttca cccgatccat tcatgatttc
     4201 cagaagttta ctatcgggag actgaaggtg aggttgagaa atgttaagga acattaagcc
     4261 actttttaaa gaacgtcagg aagggtcctg gcggacgcct ttcatggcca gggatatcct
     4321 tctacgctgc aggtctacct ccagtacaga gacccggact ttctgccctg cctttaccag
     4381 gtccgacggt tttttcacga aacgatcaga catctgactg acatgtacca gcccgtcctg
     4441 gtggacaccc acatccacga agacgccaaa ggcggtcacg ttggagacca ctcctgtaag
     4501 ctccatcccc gggttcaggt cctctatccc atgtacggaa ggagagaact ccacgctttc
     4561 aaaattcttc ctggggtccc ggccaggctt ggccaattcg gacatgatat ctctcagggt
     4621 aggtatgccc gccttttcac agacataatg agtaacattt atcctctctc tcatgctctc
     4681 gttctcaagg agatcctcga cggaacaccc caggtcggag gccatccgct cgacaataac
     4741 gtaatgatca gggtgtacag cgcttgcgtc gagagggttc acgctccctc tgatccttag
     4801 gaaccccgcg gactgttcaa aggctctggg gccgagacgc gacacctcgc gcagctgtgc
     4861 tctcgatctg aaaggcccca tggatcctct ccatgcgaca atgttagccg ccaggccggg
     4921 tccaagacct gatacgtagg acaggaggtg agggctcgcg gtgttcagtt ccacccctac
     4981 ggcgttcaca caggagacaa ccacatcctc cagtgactgc gtcagcttct tctggtccac
     5041 atcgtgctgg tactgaccga cgccgatgga tttgggatcg atctttacca gctcggccag
     5101 aggatcctgg agacgcctcc ctatcgatac ggatcccctg actgttacgt ccctgtcagg
     5161 gaactcctcc cgagcggttt tcgaagccga gtagatcgag gcaccgcttt cgttcacaag
     5221 gatcgccctg ggattgcccg gaagttccat cccctgcagg aaggccatgg tctcccttcc
     5281 cgcagttcca ttacccaccg cgactgcttt taccgggtgt ttttcgagaa tgcggcatac
     5341 ctttctcctg gcctcctcct cggccttcct gcccgaaaag gggaatatca catcatcaag
     5401 aactagccgc cccctggcat cgaggatcac cagcttgcac ccggtccgta tacccgggtc
     5461 gatggccatc acaggaaagg ctccgagcgg gggagccatc aggacctccc ttgcgttgga
     5521 ggagaagacc cctatggcct cccggtctgc ccttaccttc agggcattct ttagctcagt
     5581 ctccattgag ggtcccgcga gccgcacgta gccatccctg atggcctttc tgacttcatc
     5641 ggctgcccga ccttcggcct tgatgaacac cctctccatc tccaggaagg caagttcaga
     5701 gggcggcctt gccgatacgg tgagaaagcc ctctctctcc cctcggaaga cggccaggat
     5761 cctgtgtgag ggagtggatc tggctggctc ttcgagatcg aagtagtctc tgtacttcag
     5821 accttcctct tccctgcccc tgacaacctt cgacgatagc cttccgttgc gagcaaagac
     5881 tgccctgagt gcgaccctga cccttctgtc ctcgctggcc ctctctgcaa ttatatccct
     5941 tgctcccgca agggcatcat ctgcggactt cacgcccttg cctccgtcaa tatacctggc
     6001 tgcctctgag aggggatcaa ggtcagttct ctctcccgcg gcggcatcca cgagggcgtc
     6061 tgccagaccc ttcagcccct ttcccagcgc gatggacgcc ctggtccttc tcttcggacg
     6121 gaaggggaga tagatatcct ccagggctgt catgttcgga gctactgcaa cggctctctc
     6181 gagttcctct gtgagaaggt ccctctccct cagggatctg atcacggcag cctttctcct
     6241 gtccagctcc tccagctgtg caaatctgtc ccttatggcg agtaccgcca cctcgtccag
     6301 tgagccggta gcctccttgc gataacgggc aatgaagggc accgaacacc cttcctgcaa
     6361 aagggcgccc gtatcaagga ccttctcctc gcttatcccg agttctgccg caatgatcga
     6421 tgcatgactg gagtcagcca tcagataacc ctattccacc agcagaaggg cgaaacggtc
     6481 gggcttctcc tccctgtcct tcttgaccga tatggagtac cccagccttc tcacactgga
     6541 gtagacgtct attgcgaagg cctccatggt cggtctcctc atgtagggga gcctgcagtc
     6601 cctgggatcg gctgtgcact ctccgcagag gttgcagttg tcgaaggaga gaaggaaagc
     6661 cttttcatgt cccgccagga agacctgcct ctccgcctcc acaagcaact tctggtaact
     6721 tgcgcaccac gatcctctct cttcagagtg tacatcacag accaggtgga tcagaacagc
     6781 atgggaatac tcacccagca gctccctgca ttcctgcacg gaggggacat ggggagggca
     6841 ggaggcgttc tttccgtaat ccggacaccc gaacatgcac ttcatcctca cccactggga
     6901 aactgtcatg gacgatgggg ccacccatag acaatcgcat agaccggcct gcctgaggat
     6961 cgagtccact tccgggaagg ggctgatctt catctttctt cttccttctc cacaatagcc
     7021 ttgattattg ttacgggttc aacggggacg tcgtcgagac cggcacgact gcaggtctcg
     7081 acagacctga tcctgtcgac cacatcatgt ccttcgatca ctttcccgaa cacggtataa
     7141 ccccaccccc tgacatcagg tgatgtgaag ttcagaaagt ggttgtcctt cacattgatg
     7201 aagaactggg tcgtagcaga gtgcgggtcc tgggtcctgg ccatggcaat agtgtaggct
     7261 tcgttcctta gcccgttaga ggcctcgttc tcaacaggct cttctgttgc tttctgactc
     7321 atcgtctcgt caaaaccgcc cccctggatc ataaatccgt cgatcacccg atgaaagatg
     7381 gtcccgtcat agtgtccggt cttgacatag ttaaggaaat tctctaccgt cttcggggcc
     7441 ttttcctcga acatctctag caggatgtcg ccctcgcttg tcttcagcct gatcatccct
     7501 acacctccat tattcactgg aagatctgaa ggtccggtct cccccaaggc aaataattat
     7561 agcagagcag agtccccggg atgacaaaac cctcccggca cgttaatatc ctcccagggt
     7621 gcaaaaattc aacaattcca ggggatcctt ccatgacaag aggggaaaag ggcttcacac
     7681 ttactgaact tctgataacg atcctgatca taggcatcct ttcaggcagc ctgttcgctg
     7741 tctatggcga cgccagaagc aaggctgtag ccgcaaggat agtggccgac ctgatgaccc
     7801 tcaggagtgc ttccgcggta ttcttctccg agagagggag atggcctagc gagatcgatt
     7861 acggggacct gcttaactat ctggggagag atcccgtcgg cgagggtttg aatgagaggg
     7921 ccatcgctta cgggatcatt agccctgact ggtccccctc ggtattcata tccgccaggg
     7981 tcgggaatgg taacggagtg gcacctggaa cgagaaagat cctggaaaag gaggcttcct
     8041 ccctggggct cctggatgaa gaaggctccc cctattcttc cgggagcaat atcgtaatgg
     8101 tgatcttgaa gaaaaacgag ccctgaggaa aatgacacgg aaagccaaag acccggtatc
     8161 tcaggcctgt ctgcctcgtc cggagagcat ccgatggcca acaggtaaaa gtgacgattt
     8221 actgctgcgg tacatctgtt ttcttcccga ccaaagatcc gttgatctcc aggtgggcgc
     8281 cgtcaccctt gatccagaag gtggtcgacc cgtcagtgta tcttgcccct gacgcagata
     8341 tcccctgggg tagcgtcaga gggccatcgg gaagatagag gatcactgca ccctctatga
     8401 accgtacttc gaaaaccaga ccggaaaagt cataggtgaa aagagtgtct tccggcgctt
     8461 cgccagctcc tgcatcacaa aagaccatga atatcacgag tgacaacaat acaatagcta
     8521 cagcggacct tctcatcagc agaccctccc cgctagatcc tatctcccgg gagtattatt
     8581 gcctatgatc gtgttctgga acaacagatc ctggaaggag aatcttcatg cctaaagtga
     8641 tcgccgtctg cgcaggcaag gaccggcaga agccgaaaac agaaatggaa gctgctcact
     8701 tcgtcaaggg gaagggaata aatggtgacg ggcatttcgg gatgggggaa agacaggtaa
     8761 gccttctgag gtcagaggat atttccgagg ctgaaaacag tgccggtttc ccctttcccc
     8821 cgggatccct ggcagaaaat cttgttatag ccggacttcc ggaagacctt tctccaggac
     8881 agaccatgaa aataggagag agtgtaattc tgatcgtcgt ggaaaaaggg aaaaagcctg
     8941 gagagcctca ctcctacgat tacaggggct ggtgcctcct gcctgaaaag gggtacttct
     9001 tgagggtgga aaagggagga aaggtccaac ccggtgatcc cgtgattttg gcctgaaaag
     9061 taaacttttc cccgatggca attcctgatc tagccatttg gagaggaaaa ggtcatcctg
     9121 catgaatgat ccccatgcag cgactgttct cggcggcctg ggtgttgacc agaatgataa
     9181 ttattatcat tattttggtt gagggtgatc aatgttatcc gggggtggga ccatgacaga
     9241 taattcatca aaagaaaaat tcgaacagcg tgacttcgaa aaaccggggt ttcttttccg
     9301 tttttgcctt tcagttatcc accctttaat ttcgggtcca aggctatact cccctttcgt
     9361 cgaagggctg gaacttgagg ggagcgaacg ggtcctggag ttcgggtgcg gtaacggagt
     9421 actcacagtt tacctggcaa aagccctgga caaagggggg acagtggtcg gcgtggatac
     9481 ttcctcctac atgactgaga gggccatgac aaggctaaag ggtcattcga acgccaggat
     9541 cctgaaaggt gacatccgtt cattggggct ggagccggag agtttcgacc tggtaacgtt
     9601 cattcacgtc cttcacgaca tagcgcccgg aaaacggcag agcacaatgg ataccctttc
     9661 ttgtctcctt gcaccgggag ggcggctctg cctgatggag cccgtttctt cattccacgg
     9721 tatcccggag gagaagatac gctccatcgc caatgacgcg gggctcaagg tcactgagac
     9781 cgtgccgatg ggacggagag taaaaatttc atgcgttaaa tgacggaacc gcagtttcct
     9841 ttagataacg atttcagtgc tgagagatat ttccggctat cctttcg
//
LOCUS       DAOPKQ010000029         9745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_7307_length_9745_cov_5.792363, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000029 DAOPKQ010000000
VERSION     DAOPKQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7307_length_9745_cov_5.792363"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PK146_01955"
     CDS             complement(<1..909)
                     /locus_tag="PK146_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HPE91034.1"
                     /translation="MTRYVCIHGHFYQPPRENPWTGRVDRQRSALPYHDWNERVTIQC
                     YAPNAAARLLDGEGKVRRTLNNYSRISFNFGPTLLSWMEKASPETYAAILAADRESRE
                     RFSGHGGAIAQAYSHMIMPLASSRDKRTQVIWGVRDFMHRFGRKPEGMWLPETAVDLE
                     TLDIMTEEGIRFTVLAPHQALRVREGGTWRDVRGGKVDTTVPYSCELPSGRKMTLFFY
                     NGPIAHDIAFGELLKDGGSFARRMIDGASGEGLRLCHVAVDGETFGHHHRFGEMALAY
                     CLEKLEESRKAELTVYGEFLEKHTPKA"
     gene            complement(1380..3338)
                     /gene="glgB"
                     /locus_tag="PK146_01960"
     CDS             complement(1380..3338)
                     /gene="glgB"
                     /locus_tag="PK146_01960"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPE91035.1"
                     /translation="MRYDTSLLTEQDIYLFREGRHCRLHERLGAHFLDLPGGEGTQFA
                     VWAPAADHVSVIGDFNGWDSESFPLKPRWDSSGIWEGFVPGVGPGALYKYKISSGGSV
                     FEKGDPFAFLWETAPKTASVVWKTSYDWRDDGWMAGRGGKNSLESPISVYEVHPGSWR
                     RGGNGDFLSWREMAPLLARYAREAGFTHVELLPVMEHPFYGSWGYQTLGYFAPTSRFG
                     RPEDLMFMIDTLHQEGLGVILDWVPSHFPDDPHGLARFDGTCLFEHSDPRKGFHPDWK
                     SCIFNYGRHEVRSFLLSSASFWVDRFHADGIRVDAVASMLYLDYSRKEGEWLPNVHGG
                     NENLEAIQFLRDLNTALYRDFPGIQTFAEESTAWPMVTRPVHTGGLGFGFKWNMGWMH
                     DSLKYMSKDPVFRKYEHDSLTFSAWYAFSENFVLPLSHDEVVHGKGSLSGKMPGDEWQ
                     RFANLRLLFGYMFAHPGKKLLFMGGEFAQGREWDHDNSLDWHLAGEEPHAGVKRWVDD
                     LNRVYVEERALHELDFSREGFRWIDARDSDNSVISFLRFGKDSSEAILAVCNFTPIPR
                     YGYVLGVPFGGRWREILNSDAGLYGGSGTGNLGGVEAEGELSHGFPWRVTLTLPPLGV
                     VLLKGGEHRRQEAGKAETLEAGEPNSKA"
     gene            complement(3364..3747)
                     /locus_tag="PK146_01965"
     CDS             complement(3364..3747)
                     /locus_tag="PK146_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91036.1"
                     /translation="MTARRLKWAMALTAVLLLVLAPLAMAQVSLTGTYRGTFIGDDYG
                     WFVVEVETGGSITGVVHSNVSLMDMEVQGRWELDGAFEFMTVQEEGVPLYFLGQIDFM
                     NRFLGKWSFDDHSGRGSFYGIIQRD"
     gene            complement(3812..4342)
                     /locus_tag="PK146_01970"
     CDS             complement(3812..4342)
                     /locus_tag="PK146_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPE91037.1"
                     /translation="MQNDRINESIDHYMGLLKARGFRLTNQRRIIVETLLRNLGTHLN
                     ARELLELAQEEDSSIGIATIYRTIELLNSLGMLNMVNLEEGFLRFEVPDEQMHFHVFC
                     RSCGKTVHLDDEDKKMAEVKEWAKSEGFDLLPQTFEMAGICDACREEGVLDEPFDVVP
                     MGGRRRCRKRCGRIMR"
     gene            complement(4374..5876)
                     /locus_tag="PK146_01975"
     CDS             complement(4374..5876)
                     /locus_tag="PK146_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HPE91038.1"
                     /translation="MKRSNLLAAAAALVMAVVFCGAAFASQTAARVDVYPRGALMQMT
                     LEAVPDMQIELPSTFDPASVAVTGEGNVKVTGFDVREVVRTGWVPPSLSLLADEVEKA
                     QAQVDMLASKAASLAQAIRHLEEAVPTGLKSTELDAYVETALKKREALELRASETRVL
                     LERARKEHAALKAEYDSRFPGQPDRILLVSAATEGKGEVTLTAWTNSAYWRPLYRMGL
                     DSSTGTIEGVYGVEVNQKSGIDWDGEIAFHTVRPRGGIQIPDVPPLIADIFEPARNGK
                     VLRLESMAMAPMEDRAAGGEFLEEGLTDVTIRTSSVVYGFDEDVTIDAGTFTEQSEVS
                     LVCLPEFSPEAWTVATVKSLKRALLPGEVRLSVDGKETGTTTIRALSAGQEMEIPFGT
                     TPLVTAKREEMIPTTGTSWIIKGKHQRGYIITVSNGLAEAVTLKVKDRIPVPANESIK
                     VQDVALSPEPAERDEKGFLTWEVKLEKGQSRQISVKYTVTYPSDKELIFR"
     gene            6121..7212
                     /gene="ychF"
                     /locus_tag="PK146_01980"
     CDS             6121..7212
                     /gene="ychF"
                     /locus_tag="PK146_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPE91039.1"
                     /translation="MLSCGIVGLPLSGKSTVFNVITRAGAEVKPYAGGKTDPNRAVVS
                     VPDPRFDRLVEIWQPKKATPAPVEFVDLAGLSRDASKGAGLGNSFLSFVANSDALVHV
                     VRCFDNPDVPHPEETVDPLRDWNIVETELILRDLSVIENRLSKLADKKKPSPEEQAEK
                     LLLERCRDHLFEERPLREMTFTPEEERSLRGFTFLTLKPELVVLNLDESQAGGGMLWL
                     EDMKGLASERGLGLVSLFGRMEMEISDLPTEEQAEFMADLGITEPGRERLIREAFSLL
                     GLICFFTIGKDEVKSWTLPDGSNAVEAAGAIHSDLARGFIRAQVVHYDDFEANGFSNA
                     QCREKGLLRLEGKEYPVRDGDVIEIRFNV"
     gene            7227..8234
                     /locus_tag="PK146_01985"
     CDS             7227..8234
                     /locus_tag="PK146_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPE91040.1"
                     /translation="MTLLFEPLEIRGIRIKNRIAAPPMASLSSTKTGLPTADTLDNYI
                     PLSESGAGIAIIEHHAVHAEGRTRLRQLLLDRDEVIPYQKDLAGLFSGKGVPALAQIS
                     HAGSFIVDEDILENGWLPKAPSPIRHPLSNLFLMPVKMSESDISQIPGLFSEAARRAL
                     RAGYSGVEIHAAHGYLLGQFLSPMTNRRSDAYGGPVKNRARLLFEVYEAVRYTLGDEP
                     VVAVRLGMADTLPEAEPSGQTVDDARWVASEFSSMKLDLLDLSGNMCGYNGEGEAWFA
                     PYCRTVKDAAGKVPTICTGGIKKAETALGLLERRECDLVGVGRALKSDPDLIRKWKAL
                     E"
     gene            8231..9112
                     /locus_tag="PK146_01990"
     CDS             8231..9112
                     /locus_tag="PK146_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPE91041.1"
                     /translation="MKAIGIDLGGHFIKGALVEEGSVTTREKTPTPESRCPDEVVRTV
                     SAMISRLDPGGTARGVGVGFPGMLDRGREKVLQAPNFPLIENYPFRSRVSRACGREVY
                     LENDANCAALGEGADGAAKGLTDFAMFTLGTGIGGGIVSGGRLLTGAYGKAAELGHLA
                     VGDGTECGCGAPGHAEAVFGADALKRSFAKARIKGNLPDLWKRRDEPEVALVWGRALD
                     VLARLVASVVHVLDPQAVILGGGFSRNQGFVETLEPLVINYTAPPFRKTLDIRLSALG
                     DDAALIGATTLWGQTLK"
BASE COUNT         1955 a         3114 c         2679 g         1997 t
ORIGIN      
        1 tgccttcggg gtgtgctttt ccaggaactc accgtagacc gtcagttccg ccttccggct
       61 ctcctccagc ttttcaagac agtaggccag ggccatctcc ccgaaacggt gatggtgacc
      121 gaaggtctcc ccgtctacgg caacgtgaca gaggcgcagg ccctcccccg aggccccgtc
      181 gatcatcctc cttgcgaagg atccgccgtc ctttagcagt tctccgaagg caatgtcgtg
      241 ggctattggc ccattgtaga aaaagagggt catcttcctt ccagagggca gttcacagct
      301 gtaggggacc gtagtgtcca cctttccgcc ccggacatcg cgccatgttc ccccttccct
      361 caccctgagg gcctggtgcg gagccagtac agtgaagcgg atcccctcct cggtcatgat
      421 atcaagggtc tccaggtcca cagccgtctc cgggagccac ataccctcag gcttcctgcc
      481 gaagcggtgc atgaagtccc gaacgcccca gatcacctgg gttctcttgt ctctggagga
      541 agccagcggc atgatcatgt ggctgtaggc ctgtgcgatg gctcccccgt ggccggaaaa
      601 tctctccctg ctttccctgt cggcggccag aatggccgcg taggtctcgg ggctggcctt
      661 ctccatccag gacagcaggg ttggtccgaa gttgaaactg atgcgggaat aattgttcag
      721 ggtgcggcgc acctttcctt cgccgtccag gagccttgcc gccgcgttgg gggcgtagca
      781 ctggatggtc accctctcat tccagtcatg gtagggcagt gcgctcctct gacggtccac
      841 cctccccgtc caggggttct ccctcggggg ctggtaaaaa tgcccgtgaa tgcagacgta
      901 ccttgtcatc ttgaagcccg tgatacataa gaagtcagaa gagacaagaa ggaacacaac
      961 acccgactat gctctttcct ctcacaaaat gacccaacgc gaggaagatc cttggaagat
     1021 atcatcaaaa acgcactccc ctgaaatgat ctgataggca tttaagaact gttaagagtt
     1081 cacgaccttg ccccttgctg tcatcccgag cgaagcgaag cgggacgtcc gcctgccggc
     1141 ggagatctgg tttaaagaat tattccgatc cagattcctc acacgctttc ggcggttcgg
     1201 aatgacacag ccttgctttt ccctcttgtc ctaccctacc ctacgctggc ggtacgccag
     1261 ctagggcaga gtgaagcctc ccgaggtgat ccaacgaggg gctgcgtctg cgtcccctgc
     1321 ggtgaaaaag atttggcctt gggttcggtt tttcccgttc accgttgacc gttcaccgtt
     1381 cacgcctttg aatttggttc tccagcctca agggtttctg cctttcctgc ctcctgcctc
     1441 ctatgttctc cgcccttaag cagcaccacc ccaagggggg ggagcgtaag cgtgaccctc
     1501 cagggaaacc cgtgagagag ctcgccctcg gcttcgaccc cgccgaggtt gcccgtcccg
     1561 ctaccgccgt aaagtccggc atcgctgtta aggatctccc tccagcgccc cccgaagggt
     1621 actcccagta cataaccgta cctcgggatg ggggtgaagt tgcagaccgc cagtattgct
     1681 tcagagctgt ccttcccgaa gcgcaggaag gatatcacgc tgttgtcgct gtccctggcg
     1741 tcgatccacc tgaacccctc gcgtgagaag tccagttcgt ggagagccct ctcttccacg
     1801 tagacccggt taagatcatc cacccagcgc ttcaccccgg cgtgaggctc ttctccagcc
     1861 aggtgccagt ccagggagtt gtcatggtcc cactctctcc cctgagcgaa ctctcccccc
     1921 atgaagagga gcttcttccc ggggtgtgcg aacatgtacc caaaaaggag cctcagattg
     1981 gcgaacctct gccactcgtc gccgggcatc tttcccgaca gggacccctt cccgtggact
     2041 acctcgtcgt gggacagggg aaggacgaag ttctccgaga aggcgtacca ggctgaaaaa
     2101 gtgagcgagt cgtgctcgta cttcctgaac accgggtcct tggacatgta tttgagggag
     2161 tcgtgcatcc accccatgtt ccacttgaac ccgaacccca gcccccccgt gtggacgggc
     2221 ctggtcacca ttggccaggc cgtggactcc tcggcaaagg tctggatccc ggggaaatcc
     2281 ctgtagaggg ccgtgttaag gtccctcaaa aactggatgg cctcaaggtt ctcattgccg
     2341 ccgtggacgt tgggaagcca ctccccttcc ttccgggagt agtccaggta gagcatggag
     2401 gccacggcgt ccacccttat cccgtcagcg tgaaaccggt cgacccagaa ggaggcactg
     2461 ctgaggagga aggacctcac ctcatggcga ccgtagttga agatgcagct cttccagtcg
     2521 ggatggaaac ccttcctggg gtcggagtgc tcaaagagac aagtcccgtc gaaccgggca
     2581 aggccgtggg ggtcgtcggg gaagtgggaa ggtacccagt cgaggatcac ccccaaccct
     2641 tcctggtgca gggtgtcgat catgaacatc aggtcttcgg gtcttccaaa gcggctggtc
     2701 ggagcgaagt accccagcgt ctggtacccc caggacccgt agaatggatg ctccatgacg
     2761 ggcagaagct ccacgtgagt gaaacctgcc tccctggcgt aacgggcaag aagcggggcc
     2821 atctctctcc aggagaggaa gtctccgtta cctccccgcc tccacgatcc cgggtggacc
     2881 tcgtagaccg atatagggct ctccagggag ttctttcctc cccttccggc catccagccg
     2941 tcgtctctcc agtcgtagga cgtcttccag accaccgacg ctgtctttgg cgccgtctcc
     3001 cagaggaaag cgaaggggtc tcccttttcg aatacagacc ctccagagct gattttgtac
     3061 ttgtacaaag ctcccgggcc cacccccggc acgaagccct cccagatccc ggaggagtcc
     3121 cagcggggct tcaaggggaa gctctcgctg tcccagccgt tgaaatcccc gatgacagac
     3181 acatgatcgg ccgccggggc ccagacagca aattgggttc cctcccctcc cgggaggtca
     3241 agaaagtggg cgccgagcct ctcgtggagc cggcagtggc gcccttctct gaaaagatat
     3301 atgtcctgct ccgtcaggag cgaggtatcg tagcgcatgg tcttaccgcg gacagccagt
     3361 tcctcagtcc ctctggatta tcccgtagaa ggaaccccgt ccggagtggt cgtcgaagga
     3421 ccacttgccc aggaacctgt tcatgaaatc gatctgcccc aggaagtaga ggggtacacc
     3481 ctcctcctga acggtcatga actcgaaggc cccgtccagc tcccacctac cctgaacttc
     3541 catgtccata agcgacacat tcgaatggac cactccggtt atgctcccgc cggtctcgac
     3601 ttcaacgacg aaccacccat agtcgtcgcc gatgaaggtc cccctgtatg ttcccgtaag
     3661 cgaaacctgg gccatcgcca ggggcgccaa caccagaagc aggacagccg tcagcgccat
     3721 cgcccatttc agtctcctag cggtcattcc ttgtcacttc ctttcaaaag gttgtccggg
     3781 tttgaagatt atatcactac accgtttcat ttcagcgcat aattcttccg cagcgcttcc
     3841 tgcaccttcg ccttccaccc atggggacca cgtcgaaggg ttcgtccagg actccctcct
     3901 cgcggcacgc atcgcagatc cccgccatct cgaaggtctg gggcagcagg tcaaagccct
     3961 cgctcttcgc ccactccttc acctcagcca tctttttatc ctcgtcgtca aggtggaccg
     4021 tctttccgca ggatcggcag aagacgtgga agtgcatctg ctcgtcgggg acctcgaacc
     4081 ggaggaagcc ctcttcgaga ttcaccatgt tcagcattcc caggctgttc agcagttcta
     4141 tggtccggta gatggtggca atgccaatgg aagagtcctc ctcctgagca agctccagca
     4201 gctccctggc gttcaggtga gtgcccaggt tgcgaaggag ggtctccacg attatccgcc
     4261 tctggttcgt cagtcggaat cccctggcct tcagaagccc catgtaatga tctatgcttt
     4321 cgtttatccg gtcattctgc atccaatcgg ctcctctcct tgatggtctg cgatcagcgg
     4381 aagataagct ccttgtccga cgggtaggta actgtgtact tcacgcttat ctgtctgctc
     4441 tgcccctttt cgagcttgac ctcccaggtc aggaagccct tttcgtccct ttccgccggc
     4501 tccggcgaca gggcaacatc ctgaaccttg atggactcgt tggcgggaac tggtatccgg
     4561 tcctttacct tcagggtgac cgcctcggca agtccgttgc tcacagttat tatatacccc
     4621 cgctgatgct tgcccttgat gatccagctc gtgccagtgg tagggatcat ctcctccctc
     4681 tttgccgtga ccagaggagt ggtcccgaag ggtatctcca tctcctggcc ggcgctgaga
     4741 gctctgatcg tggtggtgcc cgtctccttg ccgtcgacgg acaacctcac ctcgcctggg
     4801 agcagggccc tcttgaggga cttcaccgtc gccaccgtcc acgcctcggg ggagaactcg
     4861 ggaaggcata caagggagac ctcggactgc tcggtgaatg tcccggcatc aatggtcaca
     4921 tcctcatcga agccgtagac caccgaactg gtcctgatcg tcacgtccgt gagcccctcc
     4981 tcaaggaact cacctccggc ggccctatcc tccatggggg ccatcgccat cgactccagc
     5041 ctcaggacct tgccgttcct tgcgggctcg aagatatcgg cgatcagcgg cggcacgtcg
     5101 gggatttgga tcccgcccct gggtctgacg gtatggaagg ctatctcacc gtcccagtcg
     5161 atgccgctct tctggttcac ctccaccccg tagacgccct cgatggtccc tgtggagctg
     5221 tcaaggccca tccggtagag gggcctccag taggcgctgt tcgtccaggc cgtaagggta
     5281 acctcaccct tgccctcggt agcggccgaa accagcagta tcctgtcggg ctgaccgggg
     5341 aagcggctgt cgtattcagc cttcagtgca gcgtgctcct tccgggccct ctcgaggagg
     5401 accctggtct ccgaggccct caactcaagg gcctctcgtt tcttcagggc cgtctcaaca
     5461 taggcatcaa gctctgtgct cttcagcccc gtaggcaccg cctcctcgag atgcctgatc
     5521 gcctgcgcca aagaggcggc cttggaggca agcatatcca cctgggcctg ggccttctcc
     5581 acctcatccg caaggaggga aagagatggc ggcacccacc ccgtccttac gacctcccgc
     5641 acatcaaagc cggtcacctt cacatttccc tcgccggtca ctgcaacaga tgcagggtca
     5701 aaggtggagg gcagctcaat ctgcatatca ggtacagcct ccagggtcat ctgcatcaac
     5761 gcgccccggg ggtacacatc cacacgcgcg gccgtctgtg aagcgaaagc cgcgccacaa
     5821 aacaccaccg ccataaccag agccgccgcc gcggccaaaa ggttcgaacg tttcatgccc
     5881 ctacctccct taaaagggtt tttggtccct catctctcat ttattcgtct tacttctcac
     5941 atctcacttc ccacggattt taaggcttcc ctgccgcctg gttttctatc tttacctttc
     6001 ccgtttacct tttaccgttc accgttgacg catttgactc tgcgtctctc attacaggtt
     6061 ctatcgatat catacacttc caccccttga gtgataaact ctttatgcga ggtgaaggca
     6121 atgctcagct gcggtatcgt aggattgccg ctcagcggca agagcacagt attcaacgtc
     6181 ataaccaggg ccggggccga agtgaaaccc tacgccggag gcaagaccga ccccaacaga
     6241 gccgtcgtct ccgtcccgga cccccgcttc gacaggcttg tcgagatctg gcagcccaaa
     6301 aaggccaccc ctgcgccggt ggagttcgta gacctggcag gcctatcccg ggacgccagc
     6361 aagggcgccg gtcttggcaa ctccttcctc tccttcgtgg cgaactccga cgccctggtc
     6421 cacgtggtaa ggtgcttcga taaccctgac gtaccgcacc ccgaggagac cgtggacccc
     6481 ttaagggact ggaacatcgt cgagaccgaa cttatcctcc gggacctttc ggtaatagag
     6541 aacagacttt caaaactggc cgacaagaaa aagccgtcac cagaggaaca ggcggaaaaa
     6601 ttgctgcttg aacgctgccg tgaccacctc ttcgaggaac gccccctgag agagatgacc
     6661 ttcacccccg aagaggaaag atccttgcgc ggcttcacct tcctcactct caagcccgaa
     6721 ctggtggtcc tcaacctcga cgaatcccag gcagggggcg gaatgctctg gctggaggac
     6781 atgaaaggac ttgcctccga aaggggactc ggccttgtca gtctcttcgg gcgaatggag
     6841 atggagatct ccgacctgcc gacggaggaa caggccgaat tcatggccga cctggggata
     6901 acagaaccgg gaagagagcg cctgatccgc gaggccttct cccttttggg gcttatatgc
     6961 ttcttcacca taggcaaaga cgaagtcaag tcctggactc tgcccgacgg gagcaacgcc
     7021 gtggaagctg ccggggccat ccactcggac ctggcccggg ggttcataag ggcccaggtg
     7081 gtacactacg acgacttcga ggccaacggc ttctccaacg cccagtgcag ggagaaaggt
     7141 ctcctcaggc tcgagggcaa ggagtacccc gtacgtgacg gggacgtaat tgagataaga
     7201 ttcaacgtct gacgggaggg aaccccatga cgcttctgtt cgaacccctg gagatccggg
     7261 gcatccgcat caaaaacagg atcgccgcgc cgcctatggc gtcgctgtcg tcaaccaaga
     7321 caggactccc caccgccgac accctcgaca actacatccc cctctccgag agcggcgcag
     7381 gaattgccat catcgagcac catgccgtac acgccgaggg gcggacacgt ctcagacagc
     7441 tactgctgga ccgcgacgag gtgatcccct accaaaagga cctggcgggg ctcttctccg
     7501 gcaagggagt cccggcgctg gcccagatca gccacgccgg ctccttcata gtagacgagg
     7561 acatcctgga gaacggatgg ctccccaagg caccctcgcc cataaggcac cctctctcca
     7621 accttttcct catgcctgtc aaaatgagcg aaagcgacat ttcacagata ccgggcctgt
     7681 tctccgaggc tgccaggaga gccctgagag caggatattc cggagtggag atccacgcag
     7741 cccatggtta cctcctggga cagtttctga gccccatgac gaaccgccgc tcagatgcct
     7801 atggaggacc tgtaaagaac agggcaaggc tcctcttcga ggtctacgaa gcggtgaggt
     7861 acaccctcgg cgatgagccc gtcgtagcag tgaggctcgg catggccgac accctgccgg
     7921 aagcggaacc ctccgggcag accgtagacg acgcccgctg ggtggcatcg gagttctcct
     7981 ccatgaaact ggacctcctg gacctgtcgg ggaacatgtg cggctacaac ggggagggag
     8041 aggcatggtt cgctccctac tgccgcactg tcaaggacgc tgcaggcaag gtgcccacga
     8101 tctgcaccgg agggataaaa aaggccgaga ccgccctggg acttctcgaa aggcgcgaat
     8161 gcgacctggt cggcgttggg agggccctca agagcgatcc cgacctgatc cgcaaatgga
     8221 aggcccttga atgaaggcga tcggcatcga tctgggggga cacttcatta agggggctct
     8281 cgtcgaagaa ggttcggtga cgacgaggga gaaaactccc acccccgaaa gccgctgccc
     8341 cgatgaggtt gtcaggaccg tttcggcaat gatctcccgc ctcgacccgg gcgggaccgc
     8401 caggggggta ggagtaggct tcccgggaat gctggaccgg gggcgcgaaa aggtcctcca
     8461 ggcccccaac ttccccctga tcgagaacta tccctttcgc tcccgcgtca gccgggcctg
     8521 cgggagagag gtttacctgg agaacgatgc caactgcgcc gccctggggg aaggggctga
     8581 cggtgccgca aagggcctga cggacttcgc tatgttcacc ctgggcacgg gcatcggcgg
     8641 ggggatagtc tccggtggac gacttctgac aggggcttac ggcaaggctg ctgaactggg
     8701 gcaccttgcc gttggtgacg gaacagagtg cggctgcgga gccccgggac acgccgaggc
     8761 cgtcttcggt gccgacgccc ttaaaaggtc cttcgcaaag gcaaggatca agggaaacct
     8821 ccccgacctc tggaaacgac gggacgaacc cgaagtcgca cttgtctggg gccgagccct
     8881 cgacgtcctg gcccgactag tggcatcagt ggtgcacgtc cttgacccgc aggccgtcat
     8941 ccttggcggc ggtttcagcc gcaaccaggg cttcgtagaa accctcgaac ccctggtgat
     9001 caattacacg gcccccccgt tccgcaaaac cctggacatc cggctctccg ccctcggcga
     9061 cgacgccgcc cttatcggcg ccaccaccct ttggggtcag accttgaaat aacgcatttg
     9121 tatacgctaa tgcgcaagaa gtaagaagaa aaacaaagat ggaaatgcgt aaaacggtta
     9181 acagtcaacg atgaacagga ggggcaaaag caaaacaaaa ttctttcacc atagacgcag
     9241 cccctcgttg aatcaccctc ctcgttaaat cgcctccctc gttacagcac ctccctcgct
     9301 acacatcctc cctcgttaga gcacctcggg acttcatcgg gaccttgtcg ggacaaggtc
     9361 cctcattgaa tcgcttcggg aggcttcgcg ctgcaccgag cgcatagtcc cgggttgatt
     9421 ctcgaggggt agcgaccagg ataggcttcg cgctgcccag agtgaaacga agaagtggaa
     9481 actggaaagt ggaattattg ggaaatccta tttaaacagg caggggggcg gctcccgccg
     9541 cccccctgta ataattggtc ctgtttctgt ttttcgtcca acgtcttacg tttgtttagt
     9601 tccaggtttt cctgttgcct gctccctgct tcccgctttt ctgtcttggg ctttccccgt
     9661 tcaccgttga ccgttcactg tttacgcctt tgaatttggt tttttcgatt cacgattcac
     9721 ggatcttgcc cttcctgcct cctgc
//
LOCUS       DAOPKQ010000030         9133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_8007_length_9133_cov_4.962877, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000030 DAOPKQ010000000
VERSION     DAOPKQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8007_length_9133_cov_4.962877"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1594
                     /locus_tag="PK146_01995"
     CDS             98..1594
                     /locus_tag="PK146_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91042.1"
                     /translation="MRKGLFFWFSILVAAGIIAGTVFFFGKVPERVVDTAMAIPAIDM
                     DSPRFFFEAGPGQLGPELPEKMVEEAEESVRSLVSGLSDLLPLATMAERSSVLGAWVA
                     NEPVFYGSFLLQPKHLKDIQEGRIPGPWLESSSGLTLGPSEREGILRLTAGKGRVVLF
                     LRGDREFLLASHSFEGLDRMAKALEGLQERFKADLSVEPLWPAHLALFDGKMIAQAAS
                     LRGLKAPDVPIALELAWNRRPDSGGIAWKAEGLKEWLPEQIREKITPMAWGGPVHFPE
                     PLIAALGVSVPEGLGGLIEKDLSVPDWMEEAGFDRSSLADLIEGPLVASVGGQSRVLL
                     FSLPGILLQLPSRGAEGIRWIEGLWSNKWARFAFSPQPVRGFNSGGRLSIPFTMIAAA
                     NEDLALAGVINDSTLGRPVPVDQVVPVGKKDAVLWFFADLPKAADALESLSRITDLAD
                     RFGVDETPDPEDLASLIGELRSMGQVTLVVHDLGSGMGSWKGAEVPAQ"
     gene            1644..2921
                     /gene="tyrS"
                     /locus_tag="PK146_02000"
     CDS             1644..2921
                     /gene="tyrS"
                     /locus_tag="PK146_02000"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPE91043.1"
                     /translation="MQGNPLAVMMERGCVEWCSHPEELSSLFDSEMVTGYIGFDPTAD
                     SLHVGHLIPMMGLAWMQSFGHRPIAIAGMGTGLIGDPSGKSKERNLLTLEQVEKNLVT
                     VKEQLGQFLDFDCGANSALIINNYDWLGKQTLLEFLRDTGKHFSVNYMIGREYVKSRL
                     DDPEKSISYTEFSYMLLQAFDFCHLYESHGCKLQMGGNDQQGNIIAGIDLIRKKAGGQ
                     AYGITYPLLLSASGQKFGKTEGGTVWLSPERTSPYRFYQFWINTDDRDVEKLLKLFTF
                     LPLDEIRDIMETHETRPEKRKAQKRLAREVTERLHGEGALRAAERASAILFGEGFEPS
                     ELTPDMLRILMSEVPTGAFPQELLPSPVEVLSSSGACKSKSEARRLIQGGGVYLNGRR
                     MGPSDEIEEGNLIEGRHLFFRLGKKRFFAASKG"
     gene            2937..3791
                     /locus_tag="PK146_02005"
     CDS             2937..3791
                     /locus_tag="PK146_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPE91044.1"
                     /translation="MSLQAHALEAVRKWIRPGWRAALASGVLYGLLRVVAPRRKVALG
                     NMEIAFPKSSPSWRRENLSRVYRHFAASLAEYLVVQNDPSLVDKWFIDSSGLEWLREQ
                     ASRGRGAVILMAHFGSWELMAAWLARRGFPIYGIIKDPDDRDLAELIEKYRRNIGGRP
                     ISKQSALKEPVRRLREGGMVVIAGDQNWGGKGALRIPFFGKDCQTAGGPAAFAIMAEV
                     PLIPVAATRLGSFRYRIDIGPPIDEPGDGTREEKMLIMTREASLWTERMIRKAPEQWL
                     WMHRRWRD"
     gene            complement(3805..3996)
                     /locus_tag="PK146_02010"
     CDS             complement(3805..3996)
                     /locus_tag="PK146_02010"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HPE91045.1"
                     /translation="MPIVQVHLLEGRSTEQKKRLVSEMTASICLALGVRPEQVRIILS
                     EMSRGDYAVGGELFSEKKI"
     gene            complement(4031..5395)
                     /gene="mnmE"
                     /locus_tag="PK146_02015"
     CDS             complement(4031..5395)
                     /gene="mnmE"
                     /locus_tag="PK146_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPE91046.1"
                     /translation="MKDIITAISTAWGESGIAIVRLSGGGSRDLVNSIFRGGEGLSLS
                     PPRFMKHGFILDNEGDPVDEVLAVWFRRPGSYTGEEAAEIHCHGGTVAARKCLELCLA
                     GGARMARPGEFTRRAFMNGRIDLAEAESVLGVIRSRSDQALRAAVKCLRGHFSARVKS
                     VNDDMADLSAFAEAGLDHPDEDIPELTPDDYLHRLYEVAQRIRDLLSSARSGRFLREG
                     IRVAISGRPNVGKSSLMNAFLEEARSIVTSMPGTTRDLIEEVVTHKGVPLRLIDTAGI
                     RDPGDLAEEEGVRRAMEAIRDADIRVWVIDGSEPITDDDRRIAMSLRGSCCIVALNKS
                     DLPVALEIDDVKDLLPGHEIRVTSAATGHGLEELKDLIVLSVSGSTSVDEDFNTTERQ
                     VEELRKALELAEGAMDAVSSGAGLDASLEMLSRSRKCLSRILGTSADEELLERIFSGF
                     CIGK"
     gene            complement(5433..6638)
                     /locus_tag="PK146_02020"
     CDS             complement(5433..6638)
                     /locus_tag="PK146_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE91047.1"
                     /translation="MGKVWEPNFSGKAQNVRPSPIREMLSVIRQPGMISFAGGMPAPD
                     VFPVKEFHEGAEVLLRDGQNLLQYGTTEGYTPLKEFLAHWTAPRMGRKVSSEEMLLTS
                     GSQQVLDLMGWAMIDPGDYIITEDPSYLAALTAFHNHGSRFIGIPTDAEGMVVDILPE
                     KIEKARSEGKKIKFIYTIVNFQNPAGCTLTLERRKKLVEISHRYGIPIFEDDPYGYVR
                     FDGEHLPSIFSLDPEGGVIYAGSFSKILAPGTRIGWCTGSMDIIRKLTVFKQALDLCS
                     SPISQALIYEYCRKGYLDRHLPNIIADYRTKRDAMEESFRKYLPLDEVTWVKPEGGFF
                     YWIHMPRIDAGELFTKAIEKKVAFVTGSPFFANGGGEHNARINYTYSRPDVIEEGVKR
                     LGEAIREML"
     gene            complement(6692..7900)
                     /locus_tag="PK146_02025"
     CDS             complement(6692..7900)
                     /locus_tag="PK146_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE91048.1"
                     /translation="MEDILADLYSLAAKSIRPSPIREMLHLVRKPGMISFAGGMPDPD
                     IFPVEQFQQAASIISREGKDILQYGTTEGYPPLKEFISEWTAPRMGRKLLPEELLITT
                     GSSQVIDLLCWALLDRGDWVICEEPTFLGATGTMYNHGARFLTVPCDSEGMKVELLPD
                     LIEKALASGNRIKFIYTIVNFHNPLGCDLSLERRKRLLEIARTYGIMILEDDPYGWVR
                     FDGEEIPSLFSMDEQGLVVFASSFSKILAPGTRVAWCAGNSEIIRKMAVFKQGTDTCT
                     SVVVQAIVWEYCRLGHLDSFLPRIVSHYRNKRDGMEAALQKNMPVGRISWQKPRGGFF
                     YWITAPGIPAVKLLERAVEKKVAFVPGNAFYPTGTGGRDNFRMCFTFASPEMTDLGVK
                     KLCEAISEFE"
     gene            complement(7979..8617)
                     /locus_tag="PK146_02030"
     CDS             complement(7979..8617)
                     /locus_tag="PK146_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPE91049.1"
                     /translation="MIRKITFPLLLLMFLAAVAVPCDAALAPETVDRIWRDLTSTASL
                     DDAGPVNIEEKEEPNAWVSFSMNKYSVHATTGLLQTLRSPDEVAGVLAHEIGHIKLGH
                     YDDTIKRNLLWMLLYKALGERRLGGIDVVGTGMVLAEAGFSREQEIAADDYGVALAAK
                     AGYDPWGLFDAFQSMVGAGFKTTPSGFNSHPPTERRMAHIRETTERISGEKR"
     gene            8782..>9133
                     /locus_tag="PK146_02035"
     CDS             8782..>9133
                     /locus_tag="PK146_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807611.1"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HPE91050.1"
                     /translation="MERLDILKNLAVENGTKIVLLVMDGLGGLPGPGGETELEAAYTP
                     NLDLLASRSDTGLLEMVDTGITPGSGPGHLGLFGYDPLKHQIGRGILEAIGVGAEVNP
                     GEICARGNLCTWGER"
BASE COUNT         1943 a         2529 c         2646 g         2015 t
ORIGIN      
        1 ggtctcctag ctggcgggac catggccatc ttcattatgc ctcccgtcta caaggtcatt
       61 tgggatatca agaaccgatt ccagaggagg gatgaaaatg cgtaaggggt tgttcttctg
      121 gttctccatc ctcgtcgcgg ctggcattat cgccggaacg gtcttcttct tcggcaaagt
      181 tccggaaagg gtcgttgata ccgcaatggc cattccggca atagatatgg attcaccaag
      241 gttctttttc gaggcaggac ccggacagct gggaccggag ctgccagaga agatggtcga
      301 ggaggcagag gagagcgtcc ggtctctggt ctcaggcctc tcggaccttc tcccgctggc
      361 caccatggca gaaagatcat cggtcctggg agcctgggtc gccaacgagc ccgtctttta
      421 tggttccttc cttttacagc ccaagcacct taaggatatt caggaaggaa ggatacccgg
      481 cccctggctt gaaagttcct cgggcctgac cctgggccca tctgaaagag aagggatcct
      541 gcgcctgacc gccggtaaag ggagagtggt cctctttctt cgcggcgaca gggaattcct
      601 tctcgcctcc cattccttcg aaggtctcga tagaatggcg aaagcccttg aaggcttgca
      661 ggaaaggttc aaggcagatc tcagcgtcga gcccttatgg cctgctcacc ttgctttatt
      721 tgacgggaaa atgatcgccc aggccgcctc cctgagaggg ttaaaagccc ccgatgtccc
      781 cattgcgctg gagctggcat ggaaccgtcg accggatagc ggcgggatag catggaaggc
      841 agagggtcta aaggaatggt tgcctgagca gatccgcgaa aagatcaccc ccatggcatg
      901 gggcggcccg gtacatttcc cggagcccct tatagctgcc ttgggagtct ccgtaccgga
      961 gggtctggga ggactgatcg agaaagacct ttcggttcct gactggatgg aggaggccgg
     1021 atttgaccgc agttcactgg ccgacctgat cgagggtcca ttggtggcct ccgtcggagg
     1081 tcagagcagg gtccttcttt tcagtctccc cggcattctt ctgcagctcc cttcaagggg
     1141 tgccgaaggg atcagatgga tcgaaggtct ctggagcaac aagtgggcca ggtttgcctt
     1201 ctctccccag cccgtccggg gtttcaattc cggagggaga ctttccattc catttacgat
     1261 gatagctgca gctaatgaag atttggctct ggcgggtgtg ataaacgatt cgacactggg
     1321 gagaccggta cctgtggacc aggttgtccc tgtcggcaag aaagatgctg tactctggtt
     1381 tttcgccgat ctgccgaaag ccgctgatgc tctcgaaagc ctttcgagga taactgatct
     1441 tgcagatcgt ttcggggttg acgagacacc cgatcccgag gatcttgcca gtctgatcgg
     1501 agagttgcgg tccatggggc aggtaaccct ggtggtccat gatctggggt cagggatggg
     1561 cagctggaag ggagctgaag ttccggccca gtaaggtccg gaacggaagg accatagaag
     1621 gttcaggaca aggaggaatc gcaatgcagg gaaaccccct ggccgtaatg atggaacgag
     1681 gctgcgttga gtggtgcagt catcccgagg aactgtcatc cctgttcgac agcgagatgg
     1741 taaccggtta catcgggttc gatccgaccg cggacagcct tcacgtagga caccttatcc
     1801 cgatgatggg gctggcctgg atgcagagct tcggtcatag gcctattgcc atagccggta
     1861 tgggcacggg gcttatcggt gacccttccg gaaagagcaa ggagaggaac cttcttaccc
     1921 tggagcaggt cgaaaagaac ctggtgaccg taaaggagca gcttggacag ttccttgact
     1981 tcgactgcgg tgccaactcg gctctgataa tcaacaacta cgactggctg ggcaaacaga
     2041 ccctgcttga gttcctgagg gacacaggca agcacttcag cgtgaattac atgataggaa
     2101 gggagtacgt caaaagtcgc cttgatgacc ctgagaagtc catctcctac accgagttct
     2161 cctatatgct tctacaggcc tttgatttct gccatcttta cgaaagccac ggctgcaagc
     2221 tccagatggg tgggaacgac cagcagggca atataatcgc cgggatcgac ctgataagga
     2281 agaaggccgg aggtcaggca tacgggataa cctacccgct cctgctgagc gcctcgggcc
     2341 agaagttcgg gaagacagaa ggcgggacgg tgtggctctc ccctgaaagg acatcaccat
     2401 acaggttcta ccagttctgg atcaacactg acgaccgtga tgttgagaag ctgctgaagc
     2461 tcttcacctt tcttcccctc gacgagatca gggacataat ggaaacccac gagaccaggc
     2521 ctgagaagag gaaggcccag aagagactgg caagggaggt aacggagaga cttcacgggg
     2581 agggtgcctt aagggccgca gagagggcca gtgccattct tttcggtgag ggtttcgagc
     2641 cctcggagct cacacctgat atgctccgga tccttatgtc tgaagtaccg acgggagctt
     2701 tcccccagga gctcctgcct tctccggtgg aggttctctc atcctccggg gcctgcaaaa
     2761 gcaagagcga agccaggagg ctcatccagg gtgggggggt ctacctcaac ggaagacgga
     2821 tgggtccatc cgacgagatc gaggaaggga acctgatcga aggaaggcat ctatttttcc
     2881 gattggggaa gaagcgcttc tttgcggcca gcaagggcta gagtgggtgg agaagcatgt
     2941 cccttcaggc ccatgccctg gaagcagtca ggaaatggat ccggcccggg tggagagccg
     3001 ccctggcctc gggagtgctc tatggacttc tgagagtggt cgcaccacga aggaaagtag
     3061 ccctagggaa catggagatc gcctttccca agtcctcccc gtcctggagg agagagaacc
     3121 tttcaagggt ttacaggcac ttcgctgcct cactggctga atatctggtg gtccagaacg
     3181 acccctccct ggtggataaa tggttcatcg attccagcgg tctcgagtgg ctccgggaac
     3241 aggcatccag aggcaggggc gccgttatcc tcatggccca ctttggaagc tgggagctaa
     3301 tggcggcctg gctggcccgg agaggtttcc ctatctacgg gatcatcaag gaccccgatg
     3361 acagggacct ggccgaactg atcgagaagt accgaagaaa tatagggggg agacccatct
     3421 ccaagcaatc cgctctcaag gagcctgtca ggagactccg ggagggcgga atggtcgtga
     3481 tagccggaga ccagaactgg ggcggaaagg gagcgctaag gatacccttt ttcgggaaag
     3541 attgccagac cgccggaggc ccggccgcgt tcgcgataat ggccgaagtt ccgcttatcc
     3601 ccgtcgcagc caccaggctg ggttctttcc ggtacaggat agatatcggt ccccccatag
     3661 acgaacccgg cgatgggacc agggaagaga agatgctcat aatgacacgg gaagcaagcc
     3721 tctggacaga aagaatgata aggaaagcgc cggagcagtg gctgtggatg caccggcgct
     3781 ggcgggactg agacagcctg gaaactaaat cttcttttcc gagaagagtt cccctccgac
     3841 agcatagtca ccgcgagaca tttcggagag gattatcctg acctgctcag gtctgacccc
     3901 aagtgcaaga cagatggaag cggtcatttc gctgacgagc ctcttcttct gctccgtgga
     3961 cctgccctcc agcaggtgta cctggactat cggcatattt tcacctcctc ggttcaccgg
     4021 atcgatctgt tcactttccg atacaaaaac cggaaaagat cctttcaaga agttcctcat
     4081 cggctgatgt cccaaggatc cttgagaggc acttccttga acgggagagc atctcaaggg
     4141 aagcgtccag accggccccg gaggagactg cgtccatagc cccctctgcc agttcgaggg
     4201 cttttctcaa ctcctcgacc tgccgttccg tggtgttgaa atcttcgtca acggatgtac
     4261 tccccgatac ggagaggacg atcaggtcct tcagctcttc cagcccatgc cctgtcgcag
     4321 ctgatgtaac cctgatctcg tgccctggca gaaggtcctt gacgtcatcg atctccaacg
     4381 ccactggaag gtccgatttg ttcagcgcta caatacagca gcttccacgg agggacatgg
     4441 cgatcctcct gtcgtcatcg gtgatgggct cgcttccgtc gatcacccat accctgatgt
     4501 cggcgtcgcg gatggcctcc atggcacgtc gtactccttc ctcttcggca aggtctcccg
     4561 ggtcccttat cccggcggta tctatcagcc tgagaggaac ccccttgtgg gttaccactt
     4621 cctcgataag gtcccttgtg gtaccgggca ttgaagtgac gatggagcgt gcttcttcga
     4681 ggaaggcgtt catcagggag gattttccta cgttcggacg gccggagatc gcgaccctga
     4741 tcccttcacg aagaaaccga cccgaacggg ccgacgaaag gaggtccctt atacgctgtg
     4801 ccacctcgta gagacggtga aggtagtcat caggcgtcaa ttccgggatg tcctcgtccg
     4861 ggtggtccag gccggcttct gcaaaggcag agagatcggc catgtcatcg ttgacgcttt
     4921 tcactctggc ggagaaatgg ccccggaggc atttgactgc cgccctgagt gcttggtcgg
     4981 aacgggatct gatcacccca agtaccgatt ccgcctctgc caggtcgatc ctgccgttca
     5041 taaaggcccg cctcgtgaac tctcccggtc gggccatccg ggctcctccg gcaaggcaga
     5101 gctccaggca tttccttgct gcaacggtgc ctccatgaca gtggatctct gcagcttcct
     5161 caccggtgta gctgccgggc ctcctgaacc agaccgccag gacctcgtct acgggatcgc
     5221 cttcattatc cagaataaag ccgtgtttca taaaacgggg cggagagagg ctcaggccct
     5281 ctccgcccct gaagatggag ttcaccaggt ccctggaccc gcccccggag agacggacga
     5341 tggctatgcc cgattcgccc caggctgtgg aaatggctgt aataatatcc ttcatggaag
     5401 gtacgggtcc tcctgttctt caaagatctc tatcagagca tctcccttat ggcttcgccg
     5461 agacgtttga ccccttcctc gataacatcc ggtctggagt aggtgtagtt gatccttgca
     5521 ttatgctcac cgcctccgtt ggcgaagaag ggggatccgg tcacaaaggc caccttcttc
     5581 tcgatggcct ttgtgaaaag ttcaccggcg tcgatcctgg gcatatggat ccagtagaag
     5641 aaacctcctt cgggtttaac ccaggtgact tcatccaggg ggaggtactt cctgaagctt
     5701 tcctccattg catccctttt ggtccggtag tcggcgatga tattggggag gtgtctgtca
     5761 aggtagcctt ttctgcagta ctcgtagatc agcgcctggg atatggggct tgaacagagg
     5821 tccaatgcct gtttgaagac cgtcagtttc ctgatgatat ccatgctgcc tgtacaccac
     5881 ccgatcctcg ttcccggggc aaggatcttc gagaaggaac ctgcatagat cactccaccc
     5941 tcagggtcaa gagaaaagat ggagggaagg tgctcgccgt caaagcggac gtacccgtat
     6001 gggtcgtcct cgaatatggg gatcccgtac ctgtgagaga tttctaccag tttcttcctt
     6061 ctttccaggg tcaaggtgca cccggcgggg ttctggaagt tgactatggt gtagatgaac
     6121 ttgatcttct tgccttctga acgggccttc tcgatcttct cgggaagtat gtcaacgacc
     6181 atgccttccg cgtcggtggg gatcccgatg aaccgggagc cgtggttgtg aaaggcggtg
     6241 agggccgcca ggtaggaggg gtcctcggtg attatatagt cgccgggatc gatcattgcc
     6301 caacccataa ggtccaggac ctgctgcgag cccgaagtaa ggagcatttc ctccgaagat
     6361 accttcctgc ccatccttgg cgccgtccaa tgggccagaa attccttcag gggtgtgtaa
     6421 ccctctgtgg tcccgtactg caggaggttc tgaccatccc ggagtaggac ctctgcccct
     6481 tcgtggaact cctttaccgg gaagacatcc ggggccggca ttcctcccgc aaaggatatc
     6541 attcccggct ggcggatgac actcagcatc tcccgtatcg gtgaaggtcg gacattctgt
     6601 gcctttccgc tgaagttcgg ttcccaaacc ttgcccaatt caatctcctc cctaaggatc
     6661 ggatgcctct gctcttcttg gggacaggct ctcactcgaa ctcgctgatt gcttcacaga
     6721 gctttttcac gcccagatct gtcatttcgg gagatgcgaa ggtaaagcac atcctgaaat
     6781 tatcacggcc tcccgtaccg gtcggataga aggcgttccc ggggacgaag gcgacctttt
     6841 tctcgacagc tctttccagc agcttcacgg cggggatccc cggggccgtg atccagtaga
     6901 agaatccccc ccttggtttt tgccaggaga tccttccgac gggcatgttt ttttgcaggg
     6961 cagcctccat tccgtccctc ttgttcctgt agtggctaac aatacggggg agaaaggagt
     7021 cgaggtgtcc aagcctgcag tactcccata ctattgcctg caccacaacg ctggtgcagg
     7081 tgtcagtgcc ttgcttgaaa acggccattt tccttatgat ctcactgttc cccgcacacc
     7141 aggcaacacg ggtaccgggg gcaagtatct tcgagaagct tgacgcgaag accaccagtc
     7201 cctgttcgtc catggagaaa agagacggga tctcctcccc atcgaacctt acccatccgt
     7261 aggggtcatc ctcgaggatc attatcccgt aggtcctggc gatctccagg agcctcttcc
     7321 ttctctccag agacaggtcg cagccaaggg ggttgtggaa gttgacaatg gtgtaaatga
     7381 acttgatacg gttcccggag gccagggctt tttcgataag atccgggagc agttccacct
     7441 tcatgccttc gctgtcgcag gggaccgtca ggaacctggc cccgtggttg tacatggtcc
     7501 ccgtcgctcc caggaaggtc ggctcctcgc agatgaccca gtcccccctg tcgaggaggg
     7561 cccagcagag aagatctata acctgagaag atcccgtggt gatcaacagt tcttccggaa
     7621 gaagcttccg ccccatcctg ggggcggtcc attccgagat aaattccttg agggggggat
     7681 aaccctcggt cgttccgtac tggagaatat ccttcccctc ccttgagatt atcgaggcgg
     7741 cctgctggaa ctgctccaca gggaagatgt ccggatcggg catccccccg gcgaaggata
     7801 tcattcccgg ctttctgaca agatgcaaca tctcccggat gggagagggt ctgatgctct
     7861 ttgccgcaag actgtaaaga tcggcaagta tgtcctccac tcccagcacc tcccgtctag
     7921 gtaaggggga gggccatgga ccctatcccc atcgatattg tatatcatta tccgcttcct
     7981 accttttctc tccgcttatc ctttcagtgg tctccctgat atgggccatg cgacgctccg
     8041 tcggggggtg ggagttaaaa ccgcttggtg tggtcttgaa tcccgcccca accatgctct
     8101 gaaaggcatc gaaaagtccc caggggtcgt aacccgcttt tgctgccaaa gccactccgt
     8161 agtcgtcagc ggctatctcc tgctcacggc tgaaaccggc ttcggctagc accatccccg
     8221 tgcctaccac atcgattccg cccagcctcc tttctcccag ggccttgtag agtaacatcc
     8281 agagaaggtt tctcttgatg gtgtcgtcgt agtgtcccag tttgatatgt cctatctcat
     8341 gggccagtac tccggccact tcatcggggg atctgagggt ctgaaggaga ccggtcgtgg
     8401 catgaacgga gtatttgttc atgctgaaag agacccacgc gttgggctct tccttttcct
     8461 ctatgttcac ggggccggcg tcatccagtg aagccgtcga ggtaaggtcg cgccatattc
     8521 tgtccactgt ctccggggca agggccgcat cacaggggac agcgactgct gccaggaaca
     8581 tgagaagaag caatgggaaa gtgatctttc tgatcatggg gcatacctcc cttcggctag
     8641 cctccgacca ttctgcagct ggacagctgc agaaagaaag ggctaaatgg cttattatat
     8701 tattcagcaa gagagggaaa aaaggcaagg gaggcttcgc cttccatggg gaggtccctt
     8761 cgcgagggag gaacaacgct gatggaaaga ctggatatcc tgaaaaatct ggccgtggag
     8821 aacggtacga aaattgtgct tctcgtcatg gatggactag gaggacttcc ggggcccgga
     8881 ggcgagacgg agctggaggc agcgtacact cctaatcttg accttctggc ctcccgttcg
     8941 gacacgggtc ttctcgagat ggttgatacg gggatcacac ccgggagtgg acccgggcac
     9001 ctcggtctgt ttggctacga tcctttgaaa caccagatag gcagagggat cctggaggcg
     9061 ataggtgtag gcgccgaggt caatccaggg gagatatgcg caagggggaa tctttgtacc
     9121 tggggcgaaa ggg
//
LOCUS       DAOPKQ010000031         8935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_8261_length_8935_cov_5.106194, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000031 DAOPKQ010000000
VERSION     DAOPKQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8261_length_8935_cov_5.106194"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PK146_02040"
     CDS             complement(<1..175)
                     /locus_tag="PK146_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPE91051.1"
                     /translation="MCLAVPHRIIALRATNRALALAGAVETEIRTDLVPGLGEGDLVL
                     VHAGFAIERLLPEE"
     gene            296..1090
                     /locus_tag="PK146_02045"
     CDS             296..1090
                     /locus_tag="PK146_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPE91052.1"
                     /translation="MDNPRVGILQLEVIRDDAAKNARYVLGQIRDAARAGCDVLVLPE
                     LWNVAFPLLGSQVLISEDNPLVGSIANAAFRNETWIIAGSMAISTEAGPRNRCLVFSP
                     DGEVSFRYDKVHLYPGLDEPNILSPGVSPGRFDLGGVPCGILICFDVEFPELARRLSS
                     GGARILFVPGAWKTEFIRFWRALLLSRAIENQVFVVGVNRCDRGRNSSFGGQSLVVDP
                     FGDVILHMDDAPRFQRVVLDLSRVDSARREHRVVSSWRPEVYRRWN"
     gene            1373..2701
                     /locus_tag="PK146_02050"
     CDS             1373..2701
                     /locus_tag="PK146_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPE91053.1"
                     /translation="MSNGTQREQWGTKLGFILACAGSAVGLGNIWRFPYITGQYGGAA
                     FVLVYVALVFVIGFSVMLAEMAIGRNAQLNAVGSFRKIKGGMWPIVGWMGVIAGFIIL
                     SYYGVIAGWTMAYVIKSFTGLMQADAGAEFGAFVTNLPQVVAWQALFMAITIYIVYKG
                     IGGGIEKYCRVLMPALFVLLLILIVRSVTLPGAGEGINFYLKPDFSKLTGGALGAALG
                     QAFFSLSLGMGCIITYGSYLGKKETLPGAAMQVCFLDTMVAFLAGLVIFPAVFAFGVE
                     PGAGPGLTFITLPNVFGQMPGGAIWSAMFFVLLFVAALTSAISLLEVVSAYFIDEMKW
                     SRPKASWIMGIVIFLLGIPSAMGIAGIIPKLAGRDFLDSADFLASNVLLPLGGFFIAL
                     FVGWFWTEGAKQEVENNGTAPFGLYTAWLWVCRVVAPLAIAYIFITGLKW"
     regulatory      2816..2925
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3076..3417
                     /locus_tag="PK146_02055"
     CDS             3076..3417
                     /locus_tag="PK146_02055"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HPE91054.1"
                     /translation="MSSKATFVEGGPVCVLTLARDRLHAGWQLLTHPLYGNVQPSQQP
                     FRSILVGLSEGSNSVDHDSLSILERAIGLYRSTGGSWDKTGEKHLLLEDFADLDVHLM
                     RDSLEKYGLLR"
     gene            3495..3812
                     /locus_tag="PK146_02060"
     CDS             3495..3812
                     /locus_tag="PK146_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPE91055.1"
                     /translation="MIELNKDNFEDEVLKSDLPVLVDLWGPKCGPCLALMPQVEEISH
                     EFEGKVKFCKLNVAENRKLVISLRVMGVPTFLFYKGGEIVDRVSGAEAVPDAIRAKVE
                     NLA"
     gene            3847..5133
                     /locus_tag="PK146_02065"
     CDS             3847..5133
                     /locus_tag="PK146_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HPE91056.1"
                     /translation="MKLKIQRVTVKALSEGDKTSFDAGTLKVNRQEIIDMLMKDQRFS
                     SVRVELARPGESARIIPVKDVVEPRCKVEGSGEVFPGFIGDVETVGQGTTLVLGGAAV
                     VTCGKIVGFQEGIIDMSGPGADYTPFSKTFNVVLVVEPVEGLEKHDYESACRLAGLKV
                     AHFLASAAKETSEGEVATYELPDLARAMTSFPGLPKVAYLYMLQSQGLLHDTYVYGVD
                     AKRIIPTLIHPNEVMDGAIVSGNCVSACDKNSTYSHQNNPIIHHMYRKHGKDFNFVGC
                     IITNENTTLADKKRSSSYAVKLAKMLGVEGVIISEEGFGNPDTDLIMNCRKAEQSGIK
                     TVLVTDEYAGRDGASQSLADACPEADAVVTAANANETIVLPEMERVIGLPDTANVIAG
                     GFEGSLREDGSIEVEIQAITGATSELGLSRIGAYTI"
     gene            5183..5659
                     /gene="grdA"
                     /locus_tag="PK146_02070"
     CDS             5183..5659
                     /gene="grdA"
                     /locus_tag="PK146_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084460285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5312..5314,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HPE91057.1"
                     /translation="MGKLAGKKVLLLGERDGVAAPAMEESLKGCGADIVFSVTECFVU
                     TAAGAMDLQNQQRVKDAAEKYGAENLVVILGSSDAEGAEIYAETVTNGDPTFAGPLAG
                     VPLGLAVYHVFEQDIRDEADPSVWEEQIGMMEMVLDTEGLANAVKGVREQYGKYTL"
     gene            5694..7001
                     /gene="grdB"
                     /locus_tag="PK146_02075"
     CDS             5694..7001
                     /gene="grdB"
                     /locus_tag="PK146_02075"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:6738..6740,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HPE91058.1"
                     /translation="MAYKVVHYINQFFAGIGGEEMAGHKPEARKGFVGPGQALNAVLG
                     PEAEIVGTVICGDTYFADNLEKAEKEVLDLIASFAPDAVIAGPAFNAGRYGTACGAVC
                     EAVGKNLEIPTVSGMYPENPGVDMYRKSTYIARTADSARGMKDAIPLMAGLLLKQLKG
                     EKMGVPDEEGYIERGVRVNCFMDKIGAERAVDMFVRKLKGEPFTTEYPMPSFDRVPPN
                     PPVADMRNARIALVTSGGIVPKGNPDHIEASSASKYGEYSLAGVLKIDSSTHETAHGG
                     YDPTYANSDPNRVLPVDAMRELEKEGVFRKFHETFYATVGNGTSVGNARRFGAEIAQK
                     LRNDNVDAVIVTSTUGTCTRCGATIVKEIERAGLPVVHVCTIVPISLTVGANRIVPAV
                     AIPHPLGDPSRTSEEEKKIRRALVEKALKALETPVEDQTVFSD"
     gene            7070..8605
                     /gene="grdC"
                     /locus_tag="PK146_02080"
     CDS             7070..8605
                     /gene="grdC"
                     /locus_tag="PK146_02080"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HPE91059.1"
                     /translation="MARVAIRGYSYCLSHTPELALHYGNTPYMERVSHGETEYLKKLA
                     RSMQSFDEACSYAPNQAFIGGIDLDTLAGQPAPMYQNRLEGASRFGRFGEIMPENEFL
                     GLIDICDVFDIIWLEGSFAADVSRELEKHPLMNGKLMSRLEKGHDSSEIEKETEEHGA
                     LPLLCQGRVVGCCRKGHDFDESLSAHVLLENIASKAGSVLALLHLLKTTGLSPTDIDY
                     VIECSEEAAGDMNQRGGGNFAKAVAEIAGCANASGFDVRSFCAGPVNAVIAGATQVAS
                     GARPNVVVLAGGAIPKLFMNSRDHVRKGIPPLEDCLGSFAVLLDADDGTCPVMRLDAL
                     GKHSVGAGASPQAITSALVYEPLQKIGLSFGDVDKFAAELQIPEITVPAGAGDVPAAN
                     FKMIAALAVMKGELKKEEMASFVESKGIPGFAHTQGHIPSGVPFIGHACEAIKAARYK
                     RVMIIGKGSLFLARLTNLSDGASFLIEPSSGRSGEARGVTPEEVRGILLEALEDLSKE
                     LTG"
     gene            8611..>8935
                     /locus_tag="PK146_02085"
     CDS             8611..>8935
                     /locus_tag="PK146_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HPE91060.1"
                     /translation="MTGTETKRIIGEALRDIVQSAKSGGPLIRVGLMATGSELGPEEL
                     ARGAVAAMEARPGLEVVMIGPRIGGYGQLQWIETPDCEEDTSRAMESALKEGDISAAV
                     ALHYPF"
BASE COUNT         1944 a         2420 c         2691 g         1880 t
ORIGIN      
        1 cctcttcagg gaggagcctc tcgatcgcga aaccggcatg gacaagcact agatcaccct
       61 ccccgagacc ggggacaagg tcggtcctga tctcggtctc caccgccccc gcaagggcca
      121 aggccctgtt cgtggctctc aatgcgatta tcctgtgcgg aaccgccaga cacattgtca
      181 tcccactcct ttgaagacag ccatgaacgg atccctgtag ctgaaccttt caagtttatc
      241 attgaacttg cccgttcgtg cctcaagatg tagtctaaca gaatggaggt gggggatgga
      301 taatcccagg gttggcatcc ttcagcttga agtgatccgc gatgatgcgg ccaaaaacgc
      361 cagatatgtc ctcggccaga tccgcgacgc tgccagggcc gggtgcgatg ttctggtcct
      421 tccggagctc tggaacgttg cgttccccct tctaggttcc caggtcctta tttcggaaga
      481 taaccctctt gtaggatcga tcgcaaatgc tgccttcagg aacgagacat ggatcatagc
      541 cggcagcatg gccatatcga ccgaggcggg gccaaggaac aggtgcctcg tcttttctcc
      601 cgacggagag gtttcctttc gatacgacaa ggtccacctc tacccaggtc tggatgaacc
      661 caatattctc tccccaggcg tctccccggg ccgtttcgat ctgggagggg tcccctgcgg
      721 gatcctgatc tgcttcgacg tggagtttcc cgaactggca aggcggcttt cttcgggagg
      781 ggcgaggatc ctcttcgtgc ctggcgcatg gaagacagaa tttatcaggt tctggagagc
      841 tctcctgctc tccagggcca tcgagaacca ggtcttcgtg gttggggtca acaggtgcga
      901 caggggcagg aactcatcct tcggaggcca gagcctggtg gtggacccct ttggagacgt
      961 cattctccac atggacgacg cgccccgctt ccagagggta gtccttgatc tttccagggt
     1021 tgattcagcc agaagggaac acagggtggt ctcgtcatgg agacccgagg tctacaggcg
     1081 gtggaattaa gctgactctt gtttaacatg ggaggaattt tctgaattac cttgcggatt
     1141 gactgttttt ctatttgtaa tataatgcgc aaaatttacc tggcttcccg gcccttcctc
     1201 tgcatcccga aggactcaag ggccgattgc caaaaaaatt ggacggcttt ataattacat
     1261 caagattatg aagcaaagtg aagggggtgt taacgccgat aagggaagtt ccgtttagca
     1321 tcaagggtcc atttgctggc agtcaaagcc cagtatcaag aggagggtta gcatgagcaa
     1381 cggaacgcaa cgcgaacagt ggggtaccaa gttgggattc atcctggcct gtgccggatc
     1441 tgccgtcggg ctggggaaca tctggcgttt tccttacatc acgggtcagt acgggggagc
     1501 tgcttttgtc ctggtctatg tagctcttgt ctttgttatc ggattctccg taatgcttgc
     1561 tgaaatggcc atcggccgta acgcgcagct caacgccgtc gggtccttca gaaagatcaa
     1621 gggcggcatg tggcccatcg tgggctggat gggagttatc gccggcttta tcattctctc
     1681 ctactacggt gtcatcgccg gctggacaat ggcctacgtg atcaaatcct tcacaggcct
     1741 catgcaggcg gacgctgggg ctgaattcgg ggctttcgtt accaacctgc cccaggttgt
     1801 agcctggcag gcgctgttca tggcgatcac catctatatc gtctacaagg gtatcggcgg
     1861 aggcattgag aagtactgca gggtactcat gccggcgctc ttcgtgctgc ttttgatcct
     1921 tatcgtccgt tcggtaacgc tccccggtgc cggagagggc atcaacttct atctgaagcc
     1981 ggacttcagc aagctcaccg gcggggctct cggcgccgcc ctcggccagg cattcttctc
     2041 actctccctc gggatgggat gcataatcac ctacggaagc tacctgggca agaaggagac
     2101 ccttcccggc gctgcaatgc aggtctgctt cctcgacacg atggtcgcct tcctggcggg
     2161 tcttgtcata tttcccgccg tcttcgcctt cggtgtcgag ccgggtgccg gccctgggtt
     2221 gaccttcatc acccttccca acgtcttcgg gcagatgccc ggaggggcca tatggtcggc
     2281 catgttcttc gttcttctct tcgtggcggc cctgacgtcg gccatttccc ttctcgaggt
     2341 cgtctcagcc tacttcatcg acgagatgaa gtggagccgc ccgaaggcct cgtggatcat
     2401 gggaatagtc atcttcctcc tggggatccc ctcggcgatg ggtatcgctg gcattatccc
     2461 caaactggcc ggaagagact tcctggattc agccgacttc ctcgcttcca acgtgctcct
     2521 gcctcttggc ggtttcttca tagccctctt cgtgggctgg ttctggaccg agggcgcgaa
     2581 acaggaagtg gagaacaacg gcacagcacc cttcggccta tacacagcct ggctctgggt
     2641 atgccgtgtt gtggctcccc ttgccatagc atacatcttc atcaccgggc tcaagtggta
     2701 gaggttggaa aatttttgtt cctaacctgc gggggctttt gcccccgctt tttttgtcat
     2761 cccttctcgt tgacctttgt ctcccttcat gagaaaatca ccctggagtt attggatgaa
     2821 ggtcgcaaga gataccctta tccccttcaa ggggactggg gagccgaagg ggcaagtgca
     2881 gacctgttgc atgaaactct caggcgaaag gattgcggct ggacaagtct ctggacaggc
     2941 gcggggaagc gtcgtcagat gaggggtttt cccgcagggg cattctggac agagaaccca
     3001 cctattcagt gaataggggg tttttcagtg ttccaggaga tcgagtgcct tactaacaac
     3061 gcccggcttt gaaacatctc ctcgaaagcc accttcgttg agggtggtcc tgtttgtgtt
     3121 ctaacccttg cgagagatcg cctccatgcc ggatggcagc ttctgaccca ccccctctac
     3181 gggaacgttc agccctctca acagcccttc aggagcatcc ttgtgggtct ttcagaaggg
     3241 agcaactctg tagaccatga ctctctctcc atactggaga gggccatcgg gctgtaccgc
     3301 tcgacaggtg gttcatggga caagaccggc gagaaacacc ttctcctgga ggactttgca
     3361 gatcttgacg ttcatctgat gcgtgacagc ctggagaaat acgggttgtt gaggtgagat
     3421 cgggtaagag tgtttccggg gaccagagag cagcgtcccc ttgggacgga gaataccgaa
     3481 gggaggtgaa aagagtgatc gagctgaaca aggacaactt tgaagatgag gtcctgaaga
     3541 gcgacctgcc ggtgctggtc gacctttggg gtccgaagtg cggtccctgc ctggccttga
     3601 tgccgcaggt ggaggagatc tcccacgagt tcgagggcaa ggtcaagttc tgcaagctta
     3661 acgtcgctga gaaccgcaaa cttgtcattt ccctcagagt aatgggagta ccgaccttcc
     3721 tgttctacaa ggggggggag atcgtcgaca gggtaagcgg ggcagaagca gtccctgatg
     3781 cgatcagggc aaaggtagag aatctggcct gagcgcgatc ggtatttcga aaggggggaa
     3841 acctaattga aacttaagat ccagagagtg actgtgaagg ccctttcgga aggcgacaag
     3901 acttccttcg atgccggaac tctcaaggtc aacaggcagg aaataatcga catgctgatg
     3961 aaggaccaga gattctcgag tgtcagggtt gagcttgccc ggccgggaga gagcgctagg
     4021 atcatccccg tcaaggatgt tgttgaacca aggtgcaagg tggagggctc cggagaggta
     4081 ttcccgggct ttatcggtga cgtggagacg gtggggcagg gaacgacgct ggtcctgggc
     4141 ggcgcggcgg tggtcacctg cgggaagatc gtgggttttc aggagggcat cattgatatg
     4201 tccggtcctg gagcggatta tacgcctttc tcgaagacct tcaacgttgt tctggtagtt
     4261 gagcctgtcg agggtctgga aaagcacgac tacgagtctg cctgccggct tgccggcctc
     4321 aaggtggccc acttcctggc gtcagccgcg aaggagacat ccgagggcga agtggcgacc
     4381 tacgaactcc cggacctggc cagggccatg acctctttcc cgggactccc gaaggtggcc
     4441 tacctataca tgctccagtc acagggtcta ctccacgata cttacgtcta cggtgtcgat
     4501 gccaagagga tcatcccgac actgatccat cccaacgagg ttatggacgg ggcgatcgta
     4561 tcaggaaact gcgtctcggc ctgcgacaag aacagcacct attcgcacca gaacaatccc
     4621 atcattcacc acatgtacag gaagcacggc aaggacttca actttgtcgg atgcatcatc
     4681 accaacgaga acacaaccct ggccgacaag aagcgcagct cctcctatgc cgtgaagctg
     4741 gcaaagatgc tgggagtcga aggtgtgata atcagtgagg aaggcttcgg caatcctgac
     4801 acggacctga tcatgaactg ccgcaaggcc gaacagtccg gcatcaagac cgtccttgtc
     4861 actgacgagt acgcaggaag ggacggcgca agccagtccc tggcagacgc gtgcccggaa
     4921 gccgacgctg tcgttacggc agccaacgcg aacgagacca tcgttctccc agagatggag
     4981 agggtaattg gtcttcctga tactgcgaac gttatcgccg gagggttcga gggttctcta
     5041 agggaggacg gttccataga ggtggagatc caggctataa ccggggctac cagcgagctc
     5101 ggcctcagcc ggatcggggc ctacactatc tagctgtacc tttgcttttg aaataattct
     5161 gatctcaaag gaggtgccgg atttgggcaa actcgcagga aagaaggtcc tccttctcgg
     5221 cgagagggat ggggtagcag ctcccgctat ggaggagagc ctcaagggtt gcggtgcgga
     5281 catagtattc tccgtgacgg agtgcttcgt ctgaaccgcc gcgggagcca tggacctgca
     5341 gaaccagcag cgcgtcaagg acgccgctga gaagtacggg gcggagaacc tggtagtgat
     5401 actggggtcc tccgacgccg agggagccga gatatacgca gagacggtaa ccaatggcga
     5461 tcccaccttc gcaggtcccc tggcaggagt cccgttggga ctcgcggtct accacgtttt
     5521 cgagcaggat ataagggacg aggcagatcc ttcggtctgg gaagagcaga tcgggatgat
     5581 ggagatggtt ctcgataccg agggccttgc aaacgctgtc aagggagttc gggaacaata
     5641 cgggaaatac accctttaga gggttgcccc gaagagaggg gggaaatgtt tatatggcgt
     5701 acaaggtagt ccactatatc aaccagttct ttgcaggcat cggcggcgag gagatggccg
     5761 gtcacaagcc tgaagcgagg aagggtttcg taggccctgg acaggccctg aatgcggtcc
     5821 tgggtcccga agcggagatc gtggggaccg taatatgcgg ggatacctac tttgccgaca
     5881 acctcgagaa ggccgagaaa gaagtcctcg acctgatcgc gtcctttgct cccgatgccg
     5941 tcatcgccgg tcctgctttc aacgcagggc gttacggtac tgcctgtggg gcggtatgcg
     6001 aggcggtagg aaagaacctg gagatcccga ccgtatcggg tatgtacccg gagaaccccg
     6061 gggtagacat gtaccgcaag tcgacctata tcgccagaac ggccgacagt gccaggggca
     6121 tgaaggatgc cattccttta atggccggtc ttctcctcaa gcagctcaag ggagagaaga
     6181 tgggagtgcc cgacgaggag ggctacatcg agagaggcgt gagggtgaac tgcttcatgg
     6241 acaagatagg ggccgaaagg gccgtcgata tgttcgtgag gaaattgaag ggagagccct
     6301 ttaccacaga gtaccccatg ccaagctttg acagggtccc accgaatcct ccggtggcgg
     6361 acatgaggaa tgccaggatc gccctggtga cgtccggcgg aattgtccca aagggcaatc
     6421 ctgaccatat cgaggcttcg agcgcttcaa agtacggaga gtacagcctt gccggcgttc
     6481 tgaagatcga ctcttcgacc catgagacag ctcatggtgg ctacgaccca acctatgcca
     6541 acagcgaccc caacagggtc ctccccgtgg acgcaatgcg cgagcttgaa aaggagggtg
     6601 tcttccgcaa gttccacgag accttctacg cgactgtcgg taacggtact tccgtgggca
     6661 acgcaaggcg tttcggtgcc gagatcgccc agaagctcag gaacgataac gttgatgccg
     6721 tgatcgttac ctcaacctga gggacctgca ctcgttgcgg cgcaacgatc gtcaaggaga
     6781 tagaaagggc ggggcttccc gttgtccatg tctgcactat agtccccatt tcgctaacag
     6841 tcggagcgaa caggatagtt ccggccgtgg cgattcctca tcctctggga gatccctcga
     6901 ggacctccga ggaggagaag aagatcaggc gggcccttgt cgagaaggcc ctgaaggcac
     6961 ttgagacacc cgtagaggac cagactgttt tcagcgattg agtttgccgg ggtggcccgg
     7021 gcgataagcc cgggccaccc atccacagaa tcggagggag gagccgccca tggctcgtgt
     7081 tgcgatcagg ggttattcct attgccttag ccataccccg gaactggcac ttcactacgg
     7141 gaacactcca tacatggaga gggtctccca cggagagacg gagtacctca aaaaactggc
     7201 ccggagcatg cagtccttcg atgaagcatg ttcctacgct cccaaccagg cattcatcgg
     7261 cgggatcgac ctggatactc tggcagggca gcccgcccca atgtaccaga acagactgga
     7321 gggcgcttca cgtttcggca ggttcggaga gatcatgccc gagaacgaat tcctcggcct
     7381 tatcgatatc tgtgatgttt tcgacatcat ctggctggag gggtcttttg cagccgatgt
     7441 ttcccgggaa cttgagaagc atcccctcat gaacggaaag ctgatgtcca ggctcgagaa
     7501 aggtcatgac tcctcggaga tagagaaaga gacggaagag catggagccc ttcctctcct
     7561 ctgccagggc cgggtggtag gctgctgcag gaaggggcac gatttcgacg aatcgctctc
     7621 cgctcacgtc cttcttgaaa atattgccag caaggcgggg tccgtgctgg ctcttcttca
     7681 cctgctaaag acgacgggtc tttccccgac agatatcgac tacgtcatag agtgttcaga
     7741 ggaagccgcc ggcgacatga accagcgggg aggagggaac ttcgccaagg cagtggctga
     7801 aatagcgggc tgtgcgaacg cctcggggtt tgatgtcagg agcttctgcg ccggtcctgt
     7861 caacgctgtc attgccggag ccacacaggt agcgagcgga gcgaggccca acgtggtagt
     7921 tctggcaggc ggcgccatac ccaaactctt catgaacagc cgtgaccatg tccgaaaggg
     7981 gatccccccc ctggaggact gcctgggaag cttcgccgtt ctcctggacg ctgacgacgg
     8041 gacatgcccc gtcatgagac ttgacgccct gggcaaacac tctgtcggtg cgggggcatc
     8101 cccacaggct attacttcgg ccctggtcta tgaacctctc cagaagattg gtctctcctt
     8161 cggagatgtt gacaagttcg ccgccgaact ccagatcccg gagatcacgg ttcctgccgg
     8221 ggcaggtgac gttcccgcgg ccaacttcaa gatgatcgct gctctcgctg tcatgaaggg
     8281 cgagttgaag aaggaggaga tggcatcctt cgttgagagc aagggaatcc ccggtttcgc
     8341 ccacacccag gggcacatac cctcaggggt gccctttatc ggtcatgcct gcgaggccat
     8401 aaaggcggcc agatacaaac gggtcatgat aatagggaaa ggaagcctct tccttgcaag
     8461 gcttacgaac ctctccgacg gtgcctcgtt cctcatcgaa ccctcttcgg gaaggtccgg
     8521 cgaagccagg ggagttaccc cggaggaagt acgcgggata ctccttgaag ccctggagga
     8581 tctctcgaaa gaactgacgg ggtgaaggcc atgactggaa cagagacaaa aaggatcatc
     8641 ggggaagcac tgcgcgacat cgtccagtcg gcgaaaagcg gaggaccgct cataagagtg
     8701 ggccttatgg ccaccggcag cgaacttggc cctgaagagc ttgccagggg agcagtggcg
     8761 gccatggagg caagacccgg tcttgaagtg gtgatgatcg ggccaaggat cggcggctac
     8821 ggtcagctcc aatggataga gacccctgac tgtgaagagg atacctcaag ggcaatggaa
     8881 tcggcgctga aggaagggga tatcagcgcg gcagtggccc ttcactaccc cttcc
//
LOCUS       DAOPKQ010000032         8810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_8416_length_8810_cov_4.244889, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000032 DAOPKQ010000000
VERSION     DAOPKQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8416_length_8810_cov_4.244889"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PK146_02090"
     CDS             complement(<1..898)
                     /locus_tag="PK146_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPE91061.1"
                     /translation="MNNAGSKREDRMNRMDRITVIGAGVSGRALAILASKLGYSVFVS
                     ELASVDTETRELFHRYGIVHESDGHSERILECDAMVLSSGVSPRARPVTRALEEGVAI
                     IGEVDFVAPFLKGAMIGVTGSNGKTTTTLLTGHLLKDSGFRVGVAGNVGSPLADHAFV
                     ENDVVVAELSSFQLFWAQELSLDVAVVTNLEPDHIDWHGSVEDYYQAKRKITSMLKEG
                     GSLVCQERDLPVLYLEGPPSGIVFPLRWQKESDRRHREGLLMKDDHAVMISGASEKNL
                     FRYTDVPLLGRHNLENAAMSSAT"
     gene            complement(911..1870)
                     /locus_tag="PK146_02095"
     CDS             complement(911..1870)
                     /locus_tag="PK146_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPE91062.1"
                     /translation="MWSAGVLVSVFLAEIALQGPWTVLLGKRRVIQNLKRYGPQNHLR
                     DKQNTPTMGGVVFLLITLLAGLSLFLLGRWTLRDAVLLLGFPLTGGAIGLADDLLKFF
                     RSSSEGLRSLQKFVLQLFAALVWSYVYIPDGLLVLAPGITLEGISGRSILIFFLVGAL
                     NAANISDGLDGLLSGMAAMSFAVLLILTPEGEADQLAAILGLGLSAGFLAHNFHPAKV
                     FMGDCGSHFLGGLLVSVCAARGMLLLILPLGFMMGLEVVSVIIQIIAIRGFNKKVFLM
                     SPLHHHFEMSGWSEKSVVYSFWALHAAGMVLLLKLLGDILICP"
     gene            complement(1870..3282)
                     /gene="murF"
                     /locus_tag="PK146_02100"
     CDS             complement(1870..3282)
                     /gene="murF"
                     /locus_tag="PK146_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPE91063.1"
                     /translation="MVKVSDQDVQRWTLGEAARAIGAVHTGEDVTMPAIIRIDSREVL
                     QGDLFIALTGERVDGHDFIEEALDRGASGLVCRPPARGSKLDHLLKKGIPAILSEDTD
                     GALREMSSVWLEKVNPGSVIGVTGTAGKTTTREMIRAVLAGSRRVHSPRKSYNTWIGC
                     ALTVLETPPGTDILLLELGTNRPGEIRSLAKTYHPGFGVITEVGPGHLEGLKSIRGVL
                     EAKMELLESPSLRSLSYNFDNELLSEAIRTSSGGLELLPVGYSSPMYRIVEARTDLHS
                     GSPDLVVMIDTPGGRREIRAGLFGEHYAYGMAFANVLGDHLKIQPAEQLEALSSFQAL
                     PGRGRCYLTDNGYLVIDDSYNANPVSMSAALRSLSSVRIRGKKCAVLGGMGELGENSL
                     QLHRDVSRLFESLDLVLLIGELWNSVFHRDTPPNCRIVDFGDLQGIFTASLSPGDLVL
                     FKGSRSFGVERALGILEGLQ"
     gene            complement(3270..4808)
                     /locus_tag="PK146_02105"
     CDS             complement(3270..4808)
                     /locus_tag="PK146_02105"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPE91064.1"
                     /translation="MRISDIEGILAERELLERVECPVTGKGERFAGENTEVKGIFLDS
                     RKVVAGGVFVCLKGKRFDGHDHALEASRNGAVAVISERVTPAPVMHMIVEEPRISMGF
                     VASALFGNPADSLQMIAVTGTNGKTTTAFMLRSIINVSGGRSGILGTVIYDTGAGYEV
                     EAQRTTPESPDIQAMLREMLDNGCSHCVMETSSHGLFQGRLSGCRFEGAAFTNLTPEH
                     LDFHGTMEEYFNSKLLLFRKYMRASGWIAVSNVSDPYGMRIKDLFPEQVVSFSLDRKD
                     RPDYFAEIGSMGLSGIRMDIISRKKGRLFGLSLPLTGRFNALNALGAAALSLSMGIAP
                     EAVRRGLETMPQVPGRLQCFRFSNGMTAIIDYAHTPDALQNVLLSVREVCKNKVWVVF
                     GSGGDRFKGNRPLMGKVATALADHVVITMDNPRSEDPSLIARDILAGVNAEIGSKSYP
                     EVILDRREAIYSVLDRASDGDALIIAGKGPERNIVFSDRIIPYSDEETVGEWSLKRGI
                     EWSR"
     gene            complement(4830..6473)
                     /locus_tag="PK146_02110"
     CDS             complement(4830..6473)
                     /locus_tag="PK146_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPE91065.1"
                     /translation="MKGLRKSPWLLLAAFILALLFRLYSLQVDPDPRVRSQAGSQYWA
                     RLRVTAPRGFIRDKNGIALAISTPTLSFFIDPTLWDPGNAPLLQDLIPRPRLERISGS
                     IGGRYYPIARKVDPPISDRIINLRLPGLFWVKETKRVYPQGNTLSHVLGFCDIDDRGL
                     AGAELFWDSVLYAPPEGRIIAKDARGDFVDVAIPGHTMTADNDSLVQLTIDSRLQHIL
                     ERRLREGFLQHKAEWAAAVLLEPSTGRILGMASLPDFNPNDRNTFADKESLRNNALSR
                     VFEPGSTLKPIIMANLIDNGEVSQSTTVNCRGKIRVADVTIHDIKAHGLMTPTSIISD
                     SCNVGMVTSVRNSDPFRTYSFLRRCGFGDLTGVEMPGEESGLMIPPSQWRGSVPSTIS
                     IGHGIGVTPLQLAVATSSTANGGKIMKPFIVSEIRSRGGALEYQGSPVVVSELMSART
                     AQWMREALRLAVEKGTGRSADVPGAKVAGKTGTAQVPGAGGYLKGDYTPSFVGFWPFN
                     DPKFLLLIVIGNPRAGDFLGGKVAAPVFRSIVQDIEQIS"
     gene            complement(6509..6937)
                     /locus_tag="PK146_02115"
     CDS             complement(6509..6937)
                     /locus_tag="PK146_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91066.1"
                     /translation="MNSHTLELPCANDIRTGGRGTTWILLCGAIVVLLMGLVALRLYS
                     LNLEYQLADIQRQIVLREEEQSRLEKDLAGLLNPTRVYSYARSELGMKASANMGLVKV
                     AAIRKDSPVDHEENRAGPGVIRKALVWLADSVSRKASARQ"
     gene            complement(6934..7860)
                     /gene="rsmH"
                     /locus_tag="PK146_02120"
     CDS             complement(6934..7860)
                     /gene="rsmH"
                     /locus_tag="PK146_02120"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPE91067.1"
                     /translation="MKTHIPVMLPEVCRILEEVKNLRFVVDATLGLGGYTEGVLLKWR
                     KTAVLGIDQDPQAIEVARRKLMDFGDRFRAVHGNFRDLSSILAKAGMGKPDAVLFDLG
                     VSSMQIETPDRGFSFQENGPLDMRMNILDEHQEMAGDIVNTYGMRELTTIFRKFGEDP
                     YAAQIARGIVKFRETSGPIKSTSQLVDIIRNTLPAPVQRKMGRNPARKIFQALRIAVN
                     DELEALNEALGQCEALVTGSMTVIVVSYHSLEDRIVKNRFRKWSSDGRGRLPFKRPMV
                     PGERELEENWRSRSAKLRAFHFASTAAEVTGR"
     gene            complement(7857..8282)
                     /gene="mraZ"
                     /locus_tag="PK146_02125"
     CDS             complement(7857..8282)
                     /gene="mraZ"
                     /locus_tag="PK146_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPE91068.1"
                     /translation="MLVGTYEHKFDNKGRIVLPSKFREELGESAVATVGIDQCVSIYS
                     SGRWQDLLNRFQQMSFSKGRTRDFLRVLLATAHEVQFDSSGRILVPSNLRQHAKLEQE
                     VSVIGVGDHIELWDTGSWNTYLENIRKDLPDIAEEVEGL"
     gene            complement(8466..>8810)
                     /gene="rnpB"
                     /locus_tag="PK146_02130"
     ncRNA           complement(8466..>8810)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK146_02130"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1945 a         2576 c         2179 g         2110 t
ORIGIN      
        1 aagtagcaga ggacatggca gcattctcaa ggttgtgtcg ccccagcagt ggaacatcgg
       61 tgtatctgaa aaggtttttc tcggaggcgc cggatatcat gacagcatga tcgtccttca
      121 tcagcaggcc ttccctgtgc ctcctgtcgg actccttttg ccatctcagg gggaacacga
      181 tgcccgaagg agggccctcc aggtacagga caggaaggtc ccgctcctgg cagacaaggg
      241 agcctccttc cttgagcatt gatgttatct tcctcttagc ctggtaataa tcttccacgg
      301 aaccgtgcca gtcaatgtgg tccggttcaa ggttggtcac aacggcaaca tcgaggctca
      361 gctcctgggc ccagaagagc tggaagctgc tcagctcggc cactactaca tcattttcaa
      421 cgaaagcatg atctgccagg gggcttccta cattacccgc cacccctacc ctgaagccgg
      481 aatccttcaa aagatgcccc gtcagaagag tggtagtggt cttgccgttg cttccggtca
      541 ctcctatcat tgccccctta aggaaaggag ccacgaagtc gacctctcct atgatagcca
      601 ctccttcttc cagggccctg gtaaccggtc gggccctggg ggaaactccg gagctcagga
      661 ccatggcgtc acactccagg atcctttccg aatgcccgtc cgattcatgg acgatcccgt
      721 atcgatggaa gagttcacgg gtctctgtat cgacgcttgc gagttcggag acaaatacag
      781 aataacccag ctttgaggca aggatggcga gggcccttcc gctcactccg gcgcctatta
      841 ccgtgatccg gtccatcctg ttcatccggt cctctctttt gcttcccgca ttgttcatgt
      901 ccgtaccccc ttatgggcag ataaggatat ctcccagtaa tttcaggagc aggaccattc
      961 cggcagcatg cagggcccag aaactgtata cgacggactt ctcgctccag ccggacatct
     1021 cgaaatgatg atgaaggggg ctcatcagga ataccttctt gttaaatcct cttatggcaa
     1081 tgatctgaat gataactgaa acaacctcca atcccatcat gaatcccagg ggaaggatca
     1141 gaaggagcat ccctcttgcg gcacatacag atacgagtag gcctcctagg aagtgagaac
     1201 cgcaatcccc catgaacact tttgcggggt ggaagttgtg tgccaggaaa ccggcggata
     1261 ggcccaaacc aaggatggct gcaagttggt ccgcctcacc ctcaggggtc aggatcagaa
     1321 gaactgcaaa ggacatggcg gccataccgc tcagaagtcc atcgagaccg tcggaaatat
     1381 tggcggcatt gagggccccg accaggaaga aaatcagtat cgatcttcct gatatgcctt
     1441 ccagtgtgat tcccggtgca agtaccagga gcccgtcggg aatatagaca taggaccata
     1501 ccagcgcggc gaagagttgc aggacaaact tctggagact cctcaatcct tcgctggagg
     1561 acctgaagaa cttgagaagg tcatcggcaa gtccgatagc cccccctgtc agggggaatc
     1621 cgaggagcag gactgcatcc cgcagggtcc accttccgag aagaaaaagg gaaagccccg
     1681 caaggagcgt gatcagaagg aataccaccc cgcccatggt cggggtgttc tgcttgtctc
     1741 tgagatgatt ctgaggaccg tatctcttca ggttctggat aactcttctt tttccaagaa
     1801 gtactgtcca gggtccctga agagctatct ccgccagaaa gacagatacg agtacccctg
     1861 cactccacat tactgaagac cctccaggat acccagagcc ctttcaaccc cgaaggaacg
     1921 ggaacctttg aacagcacca ggtcaccggg agagagactg gccgtgaaga tcccctgaag
     1981 atccccgaaa tcgactatcc tgcagtttgg tggagtatcc ctgtgaaaga cgctgttcca
     2041 cagttcgccg atcagaagga ccaggtccag gctctcaaag agcctggaaa cgtccctgtg
     2101 cagctgaagt gagttctccc ccagttcacc catcccgccc agaacggcac acttcttccc
     2161 ccggatacgg acagaagaaa gagaacggag ggccgcgctc atagagacgg gattggcgtt
     2221 gtagctgtcg tcgataacca ggtaaccgtt atctgtcaga taacacctgc ccctccccgg
     2281 cagtgcctga aaagaagaga gagcctctag ctgctctgcg ggctgaatct tcaggtgatc
     2341 ccccaggacg ttagcgaagg ccatcccata ggcgtaatgt tccccgaaaa gcccggccct
     2401 gatctccctt cttcctccag gggtatcgat cataacaacc aggtccgggg atccggaatg
     2461 aagatcggtc cttgcttcga ctatccggta cattgggctg ctgtatccga cgggcaagag
     2521 ctccagacct cccgaggagg tcctgatggc ttctgataac agttcgttat cgaaattata
     2581 ggacagcgac cttaacgaag gcgattcaag cagttccatc tttgcctcaa gaacccctct
     2641 gatgctcttg agaccctcaa gatgtcccgg gccgacctct gttatcacac cgaacccggg
     2701 gtgataggtc ttcgcaaggc tccttatctc cccggggcgg ttggttccaa gctcaaggag
     2761 aaggatatca gtccccggcg gggtctcaag gaccgtcaag gcacatccga tccatgtatt
     2821 gtagcttttc cttggcgaat ggactcttct tgaacctgca agaacggccc tgatcatctc
     2881 tctggtggta gtctttcctg ctgttcctgt taccccgatc accgatccag gattgacctt
     2941 ttcaagccag accgaggaca tttcgcgaag agcgccatcg gtgtcttcgg acaaaatggc
     3001 aggtatacct tttttcaaaa gatgatccag cttgcttccc cgggccgggg gccggcacac
     3061 caggccggaa gctcctctgt ccagggcctc ctcgatgaaa tcatggccgt ccactctctc
     3121 tcccgtgagg gcgatgaaca ggtctccctg aagtacctcc ctgctgtcga tacggatgat
     3181 ggccggcatt gtaacatctt ctcctgtatg aacggcccct atggccctcg ccgcttcgcc
     3241 aagggtccat ctttgcacat cctgatcact taccttgacc attctattcc cctcttcagg
     3301 gaccactccc ctactgtctc ctcatcgctg tatggaatga tcctgtctga aaaaactata
     3361 ttcctctctg ggcctttgcc cgcgataatt aacgcatctc cgtctgatgc ccggtcaagg
     3421 accgaatata ttgcttctct cctgtcaagg atcacctcag gataactctt tgaccctatt
     3481 tcagcgttaa cgccggcaag tatatcccga gcaataagag aagggtcttc gcttctcggg
     3541 ttatccatag ttatgaccac atggtcggca agtgctgtgg cgacctttcc catcagcggc
     3601 cgattgccct tgaaacggtc tcctccagat ccaaaaacta cccagacctt attcttgcat
     3661 acctccctta cagagagcag aacattctgg agggcatcgg gggtgtgggc gtaatcaatt
     3721 atggccgtca tcccgttcga aaacctgaaa cactgaaggc gccccggtac ctggggcatt
     3781 gtctccagac ctctcctgac ggcctctgga gcaataccca tggaaagtga gagcgccgcc
     3841 gccccgaggg cgttgagagc gttgaaacgg ccagttaacg gaagagagag cccaaaaaga
     3901 cggcctttct ttctggagat aatatccatt cttatgcccg aaaggcccat gctccctatc
     3961 tcggcgaaat aatcggggcg atctttcctg tccagggaga atgataccac ctgttccggg
     4021 aacagatcct tgatcctcat gccgtaagga tcagatacgt tggaaactgc aatccacccc
     4081 gaagctctca tgtattttct gaaaaggaga agctttgagt tgaagtattc ctccatggtc
     4141 ccatggaaat caaggtgttc aggggtcagg ttcgtaaatg cagctccttc gaaacggcag
     4201 cctgaaagcc tgccctggaa aagaccatga gatgatgtct ccattacgca gtggctgcat
     4261 ccattatcca gcatctccct gagcatggcc tggatgtctg gtgattcagg ggtcgttctc
     4321 tgtgcttcga cctcatatcc cgcaccggta tcgtagatta cggtccccaa tatccccgat
     4381 cttccacccg aaacattgat gatcgacctc aacatgaagg ctgttgtcgt cttgccgttg
     4441 gtaccggtaa cagcgatcat ttgcagcgaa tctgccggat tcccgaaaag ggccgatgcc
     4501 acgaatccca tggagatcct cggttcctcc actatcatgt gcattaccgg ggctggagtg
     4561 accctttccg atattacggc caccgcgcca tttcgagaag cctcaagggc atgatcatgg
     4621 ccgtcaaatc ttttcccctt taggcatacg aagacacctc ctgcaacgac ctttcttgaa
     4681 tcaaggaata tccctttcac ctcggtgttc tccccggcaa acctctcccc ttttccagta
     4741 acagggcatt ccactctttc cagaagttcc cgctcggcca gaataccttc aatatccgag
     4801 atcctcatct tcggcctctt tacctccgtt caggatatct gttctatgtc ctgtacaatg
     4861 ctcctgaaga ccggagcagc tactttgcct ccaaggaaat caccagctct tggattccct
     4921 atcacgatca ggagcaggaa tttcgggtcg ttgaagggcc agaaacctac gaatgaagga
     4981 gtatagtcgc ctttaaggta tccgccggct cccggcacct gggccgttcc tgtctttcct
     5041 gccacttttg cacctgggac atctgcagat cttccggtac ctttctctac ggccagtctc
     5101 aaggcctctc tcatccactg ggcagttcgt gccgacatga gttcactgac aacaacagga
     5161 cttccctggt attccagagc tccgcccctg ctccgtatct cggatacgat aaatggcttc
     5221 atgatctttc ccccgttcgc ggtggaagat gtggctaccg ccagctgaag aggagtaaca
     5281 cctattccgt gacctatgga tatcgtggat ggaacagaac ctctccactg cgaagggggg
     5341 atcatcaggc ctgattcttc tcctggcatc tcaacccctg tgagatcccc gaaaccacac
     5401 ctcctcaaaa acgagtaggt acggaacggg tcggagttcc tgacgctggt caccataccg
     5461 acgttgcagg aatcggaaat gatagaggtt ggggtcatca ggccgtgtgc ctttatgtcg
     5521 tggatcgtaa cgtcggcgac cctgatcttt ccccggcagt tcactgtcgt actttgtgac
     5581 acttctccgt tgtctatcag gttggccatg ataatgggct tcagtgtgga accgggttca
     5641 aatacccttg aaagtgcgtt gttcctgagg gattccttgt ctgcaaaggt gttcctgtcg
     5701 tttggattga aatcgggaag gcttgccatc cccaggatcc tgcccgtcga cggctcaaga
     5761 agaacggcag cagcccactc cgccttatgc tgaaggaacc cttcccgaag cctgcgttca
     5821 agaatgtgct gaaggcggga atcaatggtc aactggacca gtgaatcatt atcggcggtc
     5881 attgtatggc ctggaattgc tacatccaca aaatcgcccc ttgcatcctt ggcgatgatc
     5941 ctcccttcag gaggagcata aagaacggag tcccagaaga gttccgctcc tgccaggccc
     6001 ctgtcatcga tatcgcagaa tcccaggacg tggctcaggg tattgccctg ggggtatact
     6061 cttttggtct ccttgaccca gaacagacct ggaagccgca ggttgataat cctgtcggag
     6121 atgggggggt caacctttcg ggcgatcggg tagtatcgcc ccccgatgct tcccgagatc
     6181 ctttcgagtc ggggccgggg gatcaggtcc tggagaaggg gagcatttcc aggatcccag
     6241 agagtggggt cgataaagaa actaagtgtc ggagtggaga tcgccagagc gatcccgttt
     6301 ttgtccctga tgaaacctct cggcgctgta actctcaacc tggcccagta ttggcttccg
     6361 gcctgtgatc tgaccctggg gtccggatca acctggaggg aataaagcct gaagagcagc
     6421 gccaggatga atgcagcaag caggagccat gggctcttcc tcaggccttt cattcgatac
     6481 tgaccgtcct ttatccgtca atttgcggtc actgccgggc ggaagccttc ctagatacgg
     6541 aatctgccag ccacacaagg gctttccgga tcacacctgg tcctgccctg ttctcctcat
     6601 ggtccacagg cgaatccttc ctgatcgctg ctaccttgac cagtcccatg ttggcggagg
     6661 ccttcatgcc caattccgac cttgcataac tatacactct cgtaggattc agtaaacccg
     6721 caaggtcctt ttccagcctc gactgctctt cctctctcag gactatctgt ctctggatat
     6781 cagccagctg gtattcgaga ttgagggaat agagcctcag tgctaccagt cccatcagga
     6841 gaaccactat cgcaccgcag agaaggatcc aggtggtccc acgtcccccg gtccttatat
     6901 cgttcgcgca aggcagttcc agggtgtgag agttcatcgg ccggtcacct ccgcagcggt
     6961 cgaagcgaaa tgaaatgccc tgagtttggc acttctcgat ctccagttct cctccagctc
     7021 tctttcacca ggcaccatgg gcctcttgaa gggaagtctt cctcttccat ctgaagacca
     7081 cttccggaac ctgttcttga cgatccggtc ctcaagggag tggtaactca cgacgatcac
     7141 cgtcatggat cccgtaacca gcgcttcaca ctggccaagg gcttcattca gcgcctccag
     7201 ttcgtcgttg acggctattc tgagagcctg aaagatcttc ctggcgggat tccttcccat
     7261 cttcctctgg acaggcgccg gaagcgtatt cctgatgata tctaccagct gggatgtgct
     7321 ctttatgggt cccgatgttt ccctgaactt gactattcct cttgctatct gtgccgcata
     7381 gggatcttcg ccgaacttgc ggaatatggt cgtcagttct ctcatgccgt aggtgttgac
     7441 tatgtctccg gccatttcct gatgctcgtc aagaatgttc attcgcatat ccagtggacc
     7501 gttctcctgg aatgaaaaac ctcgatcggg ggtctctatc tgcatacttg atacccccag
     7561 atcgaaaaga accgcatcgg gtttccccat tccggcttta gccaggatcg acgagaggtc
     7621 ccggaagttg ccgtgtaccg cccggaatcg atccccgaaa tccataagtt ttcttcttgc
     7681 cacttcaatg gcctgaggat cctggtcgat acccagaact gccgtttttc tccatttcag
     7741 gagaaccccc tccgtataac cccccagacc cagggtggca tccacaacga acctcaggtt
     7801 cttgacctct tcaagtatcc tgcagacctc cggtagcatc acgggaatat gggtcttcat
     7861 aacccttcca cctcttccgc gatgtcggga agatccttgc gaatattctc aagataggta
     7921 ttccaggagc ccgtatccca cagttcgatg tgatccccga cacctattac gctcacttcc
     7981 tgttccagtt ttgcatgctg tctcaggttc gaggggacca ggatccttcc ggaagagtcg
     8041 aactggacct catgggcggt ggcgagcagc accctcagaa aatccctggt ccttcccttg
     8101 gagaaggaca tctgctggaa cctgttcagg aggtcctgcc atctccccga tgagtagatc
     8161 gatacgcact gatctatgcc tacagtcgcg acagcgctct cgcccaactc ttccctgaac
     8221 tttgacggaa ggacgatcct gcccttatta tcgaatttat gctcataggt tcccactagc
     8281 atgggatttt cctcccatat tatgccactt tatgccactt tgttccactt tatcaccttt
     8341 tgactgtttt caatagccct ttagtcccgt ttcattgttt tcttataatt ctctagcacc
     8401 atctttccct aaacgcaaca aaaaaggggt gcatcgctga acgatgcacc ccagatcttg
     8461 cagaccgcgg gaagcaaccc ctaagccggg ttctgtgcga ggcggtcatt tatctaggag
     8521 ccggctttcg ccggccctca agcgaccgta cccgagggtc agcgggcagc ctcatcccct
     8581 cctattcggt cttgctccgg gcggggtttg cctagcctgt cggtcacccg acagctggtg
     8641 ggctcttacc ccaccttttc acccttaccc ggcactcatt acagggtttg gaggcctgtt
     8701 gggaaataac gctttcaatg aagctggtca tggtttgctt tgcgccaaac tctataatga
     8761 gtgccgggcg gtatgtttct gtggcacttt ccttgggctc acgcccactg
//
LOCUS       DAOPKQ010000033         8570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_8733_length_8570_cov_5.041574, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000033 DAOPKQ010000000
VERSION     DAOPKQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8733_length_8570_cov_5.041574"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK146_02135"
     CDS             <1..213
                     /locus_tag="PK146_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91069.1"
                     /translation="AHAGLRVEKDLHDPEKGISAGTLLLSRYIKAYGSTERALGRYYG
                     GPVSRYWPKVARNMDRVISYGLEPGI"
     gene            complement(268..1284)
                     /gene="corA"
                     /locus_tag="PK146_02140"
     CDS             complement(268..1284)
                     /gene="corA"
                     /locus_tag="PK146_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPE91070.1"
                     /translation="MEAPPEEGPGPGPGPEINLIHFSEQSFTEKWLSSLEDLPEHSDE
                     GQTAWIDITGLNDNALLRETAERFGIHPLVLEDICNTGQRAKSEDYGDYQYTVLKMVS
                     WDEDSMQVEVEQVSMVLFPKVLITFQERPGDLFEPVRQRLRQGKGRLRKEGSDYLAYS
                     LLDAIVDGYFLLLEKIGDQVEVLEEELLSAPRTGTSARMHELRREMIFLRKAAWPLRE
                     AVASLERSGPLSGGTTAVYMRDLYDHVIQVIDGIETIRDILSGMLDIYLSTVSNRMNE
                     IMKVLTVIATIFIPLTFIAGVYGMNFALMPELQWKWGYPAVLAVMAGTALWMLAFFRR
                     KGWL"
     gene            complement(1369..2154)
                     /gene="xth"
                     /locus_tag="PK146_02145"
     CDS             complement(1369..2154)
                     /gene="xth"
                     /locus_tag="PK146_02145"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPE91071.1"
                     /translation="MGNFRIATFNVNSVRSRLPVLERWLKNNRVDCLCLQETKVEDGS
                     FPTPFFEDLGYHVVFSGEKSYNGVAVASTVPPDEYGFGLDDGEEPSGRTRIAWCRFGD
                     LRVANAYVPQGKSIDHPDYPMKLRFFKRMEAFLRSLGVPKERLVLVGDLNVAPTDIDV
                     THPENKRDHVCFHEEVREAFRSLCSTGLVDVFRKHRPGEGEFSFWDYRVKGALERNIG
                     WRIDHILATALLADVSIDSYSDREPRSWEKPSDHTAVVAEFKI"
     gene            complement(2592..3344)
                     /locus_tag="PK146_02150"
     CDS             complement(2592..3344)
                     /locus_tag="PK146_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPE91072.1"
                     /translation="MAENGKRSTNAGLVVLGIALAAGMVLSAWLVSGTVKSVKLANQT
                     IMVKGFAQKDVRSDIAVWTGRFTARDTELVRAYSRLESDLEKVRDFMSRSGIPKEEVD
                     VSAVTTMIQYRRTPQGYDTNEIEQYVLEQTVTVRSKEVDLVASLSRESTTLIREGVEF
                     FSFSPEYYYSGIEDMKIHLLGEATLNARQRAEQLAVNSGGKVGPLRAASQGVFQITPL
                     YSTDVEDWGRYDTTTIEKAVKAVVTIQYSIDS"
     gene            complement(3350..3871)
                     /locus_tag="PK146_02155"
     CDS             complement(3350..3871)
                     /locus_tag="PK146_02155"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPE91073.1"
                     /translation="MAVRRRKRSNQGVSRVGSLLMSSLSREQSMMLLFADLCSRWGEV
                     VGPKLAGRSSPGSIQDGTLTVVVSEPILSQEITMRGGDIASRISRGWGLDVSGVSVKV
                     GRTRRRSPEKRSPKREALKIGPEEVKSCLEKVRPVIPRDDVASDLARLMALYKKRFGS
                     TGGGQIRKTGKER"
     gene            complement(3843..5777)
                     /gene="mnmG"
                     /locus_tag="PK146_02160"
     CDS             complement(3843..5777)
                     /gene="mnmG"
                     /locus_tag="PK146_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPE91074.1"
                     /translation="MKGRTVQGGHLSDDRSFDVIVVGGGHAGCEAALASARMGARTLL
                     VNLLLDNAAMMPCNPSIGGPAKGHLTREIDALGGEQAAAADNSTIHIRMLNTSKGAAV
                     RTLRAQCDLRDYHLWYRRACDGQPKLEVMQDQVTDLWVERGRARGVRTRIGSVYEARA
                     VILATGTFLGGKVHIGLTSFSSGPLGQMPAEGLGASLREAGLKVGRLKTGTTPRIHTA
                     SVDWDALERQDSAAEPYCFSHWGTPRVYTGYACHATRTNEETHRAIRDSLDRSPLFTG
                     AIEGKGPRYCPSIEDRVVRFPDKESHLVFLEPVGRDSVEVYMQNFTTSLPLDAQIRMV
                     RSLPGCGRARIMRPGYAIEYDFIPPTQLEPWLEAKAIGGLFCAGQINGTSGYEEAAAQ
                     GILAGINAVLSTRREEPLVIERSRGYMGVLVDDLVTRGTEEPYRMLTSRCEYRLLLRH
                     DNADRRLAPLARKLGLLDDERWRVLQKRWDSLDRELQRLKNTRIRDRQLLEKVLEGPE
                     TGCAEPGVTAADLLKRPGVTWEMVAEAAPPVERPDPEIIERTGIEIKYEGYVARQLSQ
                     VERMKRMDLVRIPGELDYSSVTGLLNESRQKLQAIRPRTLGQAGRISGVTPADIMLLE
                     VFIERLRRGVDHGRKEKEEE"
     gene            complement(5767..6822)
                     /gene="recF"
                     /locus_tag="PK146_02165"
     CDS             complement(5767..6822)
                     /gene="recF"
                     /locus_tag="PK146_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPE91075.1"
                     /translation="MKISSTQWEGFRNLKPRRLEWAEGLHLLLGPNGSGKTNILEAFH
                     VLTGWGSLRGERTADLRSWTQPGRGTFLSAAFDGEDSLEVKVGIGARIVAKKGGSSLR
                     SSQLRPLVPSLIFLPNDLSLVEGSPATRRRLIDRLCALLFPVYALRLHEYGRALRQRN
                     ASIRMGRNLAATARVMAPLAAWIWACRLTAVDLIRNGLEEGEGLLPPGVTLELKRGGR
                     GLEDLHLDFWESLEASQKQERATGVCPVGPHRDDLVIRFQERDVARCFSRGYRRRVAI
                     ALLLSSGWAVERKMRRKPVLFLDEVMAELDHEGRERLIAVLLRTGWQVFATTAEACTP
                     DWPGRVWSVNGGRVDEG"
     gene            complement(6819..7961)
                     /gene="dnaN"
                     /locus_tag="PK146_02170"
     CDS             complement(6819..7961)
                     /gene="dnaN"
                     /locus_tag="PK146_02170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPE91076.1"
                     /translation="MKLLVDKAKFLKIWSMAERSAGHRSTISVLSGVKCEASAEGVSL
                     KATDLKTSINCLADGVEVLSEGSAIFPVKNIGEFFKKAPGDTFTIEVDKGKATVISGS
                     SVYSFSTYPVSEFPKLPSSDKAEPFFKAVCMDLRRLLEEGTFSGSTSEEFPQYLSAGL
                     VQTDEERVKVVSTDGRRLSLSQGTVTVDGEPDQVLLPLAGLRELQRSLSTVEGDEEIR
                     VLKDDSQVYFRTGTMEFSVRRVESRFPPYEKVLSSDRTSWLTIDRELFIEALERVEVV
                     VRDFSRMVVFMLSPGGTLKMQAKAPEVGEAFEEIPGDCDGEPLKIAFNVRYLLEGLKA
                     LHGTVAHLSFNGPNGQMTLSRPREDSFMYVLMPITLPEEENSEETA"
     gene            complement(8185..>8570)
                     /locus_tag="PK146_02175"
     CDS             complement(8185..>8570)
                     /locus_tag="PK146_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPE91077.1"
                     /translation="LNSVMFFADLNNESVTVDNASRWLKDLIRRSSRGQITIELIQQV
                     TAESFGIPVSSLSGNKRTSEIALARQVAMYLSRDMTGTTLQQIGYAFNRKDHTTVIHA
                     CKKIEESLRKDSRIKSIVENLREKL"
BASE COUNT         1631 a         2716 c         2334 g         1889 t
ORIGIN      
        1 gcccacgcgg gactcagggt ggaaaaggac ctccacgacc ccgagaaggg gatctcggca
       61 gggacgctgc tcctctcaag gtacatcaag gcttacggca gcaccgagcg ggcccttggc
      121 agatactacg gaggcccggt cagccgttac tggccaaagg tggccaggaa catggaccgt
      181 gtgatcagct acggtctcga acctggcatc tgaccggttt tgtccacaaa tttgttcgca
      241 acctgtggat gtctgccccg acagacccta cagccacccc ttcctcctga agaaggccag
      301 catccataac gccgtccctg ccatcacggc caggacggcg gggtaacccc acttccactg
      361 cagttcaggc atcagggcaa aattcatccc gtagaccccg gcaatgaacg tcagggggat
      421 gaagatcgtg gctatcaccg tcagtacctt catgatctcg ttcatcctgt tgctcaccgt
      481 tgaaaggtag atatcgagca tccccgaaag gatgtccctg atggtctcga tgccgtcgat
      541 gacctggatg acgtgatcgt aaaggtcgcg catgtacacc gccgtggtcc ctcccgacag
      601 aggtccgctc ctctctaggc tcgccaccgc ttccctcaac ggccaggcgg ctttcctgag
      661 aaatatcatc tcgcgcctca gttcgtgcat ccgggccgat gtccctgtcc tgggggcaga
      721 aaggagctct tcttctagga cctccacctg gtcgccgatc ttctcgagaa ggaggaagta
      781 gccatccact atggcatcga gaagcgagta ggccaggtaa tcggacccct ccttcctcaa
      841 tcgccccttg ccctgcctca gcctctggcg gacaggctcg aagaggtcac cgggcctctc
      901 ctggaaggtg atcagcacct tggggaagag caccatgctg acctgctcca cctccacctg
      961 catcgaatcc tcgtcccagg agaccatctt cagcaccgtg tactggtagt cgccgtagtc
     1021 ctcggatttg gccctctgtc cggtgttgca gatatcctcc aggaccaggg ggtgtatccc
     1081 gaacctctct gccgtctccc tcagaagggc gttgtcgttc agtccggtga tgtcgatcca
     1141 ggccgtctgg ccctcatcag agtgctccgg aaggtcttca agggatgaca gccacttctc
     1201 ggtgaaggac tgctccgaga agtggatcag gttgatctcg ggtcctggcc ctggtcccgg
     1261 tccctcctcc ggaggagctt ccatcggagg agggttcatc ctgaggtgcc ggctgatcct
     1321 ttttttacgg ttccttggca cggcaaacca cctgcctttt gaaaaccgct atattttgaa
     1381 ttctgccacc actgccgtat ggtccgaagg cttctcccat gatctgggct cccggtcgct
     1441 gtagctgtcg atggatacgt ccgccagcaa agccgtggcg aggatgtggt cgatcctcca
     1501 tccgatgttc ctctcgaggg cacccttgac cctgtaatcc cagaaggaga actccccctc
     1561 ccccgggcga tgctttctga agacgtccac caggcctgtc gagcacaggc tccgaaaggc
     1621 ctctctgacc tcctcatgga agcagacatg gtcccgtttg ttctccgggt gggtcacatc
     1681 gatatcggtg ggcgcaacgt tgaggtcgcc caccaggaca aggcgctcct tcggaacccc
     1741 cagggaccgg agaaaggcct ccatcctctt gaagaacctg agcttcatgg ggtagtcggg
     1801 gtggtcgatc gatttgccct gggggacata ggcgttcgcc accctgaggt ccccgaagcg
     1861 gcaccaggct atccgcgtcc tccctgaagg ctcctcgccg tcatcaaggc cgaagccgta
     1921 ttcatcgggg gggaccgtag aagccacggc cacgccgttg taggatttct cgccgctgaa
     1981 cacaacgtgg taacccaggt cctcgaagaa cggggtcgga aatgagccat cctccacctt
     2041 tgtctcctgg aggcagagac agtcgaccct gttgttcttc agccatcttt caaggacggg
     2101 caaccggctc ctgacggaat tcacgttgaa cgtggcgatc ctgaagtttc ccattgcaat
     2161 taccccctgt atcgttgatc gaagatcgtg agaagttaga agtcagacgt gagacgaaag
     2221 accaaagtca agcgcgtacc ctccagacaa gctgaaggac ctggtcccga ggcgtattaa
     2281 cgagggacgg gccctgtcgc tgcgctcggg gcacgttgta actcgtgact cgtaactcgt
     2341 gactcgaaaa ttcaaaacca gatccctcat ccgtcggctg gcggattcag gatgacaggt
     2401 caactgcgta aacagtgaac gcgaagtccc gcgatctctg cgggggtgaa cggagaaggc
     2461 aagaacagat cataaatccc gcacctgccc tttttaaacc ataaagaggt tcaggtcttc
     2521 tctctctttt ccctctgcgt cctctgcggt gcaaaacttt tgcttttgcc ctggcatttc
     2581 cgcgtcacgc gtcacgaatc aatcgaatac tgtatcgtta ccaccgcttt cacggccttc
     2641 tcgatggtgg tcgtgtcgta cctgccccag tcctcgacat ctgtcgagta aagaggcgtg
     2701 atctggaaga ccccctggct cgccgccctc aggggaccga ccttcccccc gctgttgacg
     2761 gccagctgtt cggctctctg ccgggcgttc agggtggcct cgcccaggag atggatcttc
     2821 atatcctcga tgccagagta gtagtactcc ggtgagaagg agaagaactc caccccctcc
     2881 ctgatcaggg tggtggactc cctggagagg gaagccacca ggtcgacctc cttcgatctg
     2941 accgtgacgg tctgctccag cacgtactgt tctatctcgt tggtgtcata accctggggg
     3001 gtcctgcggt actggatcat tgtggtgacc gccgatacat cgacctcttc cttcgggatc
     3061 ccgctccggg acatgaaatc cctgaccttt tcaaggtccg attcaagcct ggagtaggcc
     3121 ctgaccagct cggtgtctct ggcagtgaaa cgccccgtcc ataccgctat gtccgatctt
     3181 acgtccttct gggcgaaacc cttcaccatt atggtctggt tggccagttt gacgctcttg
     3241 acggtcccag aaacgagcca cgcagagagg accattccag ccgcaagggc aattccgaga
     3301 actaccagcc ccgcattggt cgacctcttt ccattctctg ccatggagat cacctttcct
     3361 ttccggtttt ccggatctgt cccccgccgg ttgagccgaa cctctttttg tataacgcca
     3421 tcagtctcgc aagatcggag gcgacgtcat ccctggggat cacggggcgg accttctcca
     3481 ggcaggactt cacttcctcg gggcctatct tcagagcttc ccttttggga gatcttttct
     3541 ccggggacct tcttctcgtc cttcccacct tcacgcttac acctgacaca tcaaggcccc
     3601 agcccctgct gatcctggag gctatgtcgc cccctctcat ggttatctcc tgggagagga
     3661 tcggttccga tacgacaacg gtcagggtcc cgtcctgtat ggaccccgga gagctccttc
     3721 cggccagttt tggacccacc acctcgcccc agcgcgagca gaggtcggca aaaagaagca
     3781 tcatcgactg ttcccttgag agcgatgaca tcaggagcga cccgaccctt gaaaccccct
     3841 ggttactcct cttccttctc cttacggcca tggtccaccc cccttcggag gcgttcaatg
     3901 aatacctcca gcaacattat atccgctggg gtcacccccg aaatcctccc ggcctgcccc
     3961 agcgtcctgg gacggatcgc ctggagcttc tgcctgctct cgttgaggag gcccgtcacc
     4021 gaggagtagt ccagctcccc gggtatgcga acgaggtcca tcctcttcat cctctccacc
     4081 tgggagagct gtcgggccac gtacccttcg tacttgatct ctatcccggt ccgttcgatt
     4141 atctccgggt caggccgctc gaccggaggt gcagcctctg ccaccatctc ccaggtgacg
     4201 cccggtcttt tcagaaggtc cgcggcggtg acccccggtt cggcacaacc agtttcagga
     4261 ccctcaagga ccttttccaa cagctgccgg tcccggatcc tggtattttt tagcctctgg
     4321 agctccctgt cgagggagtc ccacctcttt tggaggaccc tccaccgttc gtcgtccagg
     4381 agtccgagct tcctggccaa aggcgccaga cgcctgtcgg cgttgtcgtg tcttagaagg
     4441 agcctgtact cacagcggct ggtcagcatc cggtagggtt cctctgtccc cctggtgacc
     4501 agatcgtcga ccagcacccc catgtagccc cgggacctct cgatcaccag gggctcctcc
     4561 ctccttgttg acagaaccgc gttgatgccc gccaggatac cctgggcggc ggcctcttcg
     4621 tagcctgagg ttccgttgat ctgacctgcg cagaagagcc ctccgatggc ctttgcctcc
     4681 agccatggct caagctgggt gggcgggata aagtcatatt cgatggcgta accgggcctc
     4741 attatcctcg ctctcccgca cccggggagg gaccgaacca tcctgatctg ggcatccagg
     4801 gggaggctgg tggtgaagtt ctgcatatag acctccacgc tgtcccttcc gacgggctcg
     4861 agaaagacga ggtggctctc cttgtcgggg aaccttacaa ccctgtcctc gatggagggg
     4921 cagtacctgg gccccttccc ctcgatagcc ccggtgaaca ggggggaccg gtccagcgaa
     4981 tcccttatgg cgcggtgggt ctcttcattg gtcctggtgg cgtggcaggc gtagccggta
     5041 tagacccttg gggtccccca gtggctgaag cagtagggtt cggcggcgct gtcctgcctc
     5101 tcgagagcgt cccagtccac gctggcggtg tggatccttg gggtggtacc ggtcttgagc
     5161 cttccgacct taagtccggc ctcgcgcagg ctcgccccca gcccctcggc gggcatctgt
     5221 cccagcgggc ccgatgaaaa agaggtcaat cctatgtgga ccttccctcc caggaaggtc
     5281 cccgtggcca ggatcaccgc ccgggcctcg tagaccgatc ctattcttgt cctgactcct
     5341 cttgcccttc ctctctccac ccagaggtcc gtgacctggt cctgcatcac ctcaagtttc
     5401 ggttgcccgt cacaggctct ccggtaccag aggtggtagt ccctcaggtc gcactgggct
     5461 ctcagggtcc tgacggccgc ccccttggag gtgttcagca tccggatatg tatggtcgag
     5521 ttgtcggcag cggctgcctg ctcccccccc agggcgtcga tctccctggt caggtgcccc
     5581 ttggctggtc cccctatgga ggggttgcag ggcatcatgg cagcattgtc caaaagcagg
     5641 ttgacaagga gcgttcttgc ccccattctg gccgaggcca gggcagcctc gcacccggcg
     5701 tgccccccac cgactacgat gacgtcgaac gaccggtcgt ccgacagatg ccccccctga
     5761 acagttctac ccttcatcga cacggcctcc attgacggac cagaccctcc ccggccaatc
     5821 gggggtacag gcctccgccg tggtggcgaa gacctgccac cccgtacgga gcaggacggc
     5881 aataagcctc tcccttcctt cgtggtccag ttcagccatg acctcatcga ggaaaaggac
     5941 cggcttcctc ctcatcttcc tctccacggc ccaccccgac gacagcagga gggctatggc
     6001 tacccttctc ctgtagcctc ggctgaagca ccttgccacg tccctctcct ggaaacggat
     6061 cacaaggtcg tccctgtggg gtccgacagg gcatacgcca gtagccctct cctgtttctg
     6121 actggcctcg agggactccc agaaatccag gtggaggtcc tcaagccctc tgccacccct
     6181 ttttagttcc agggtcacac caggggggag caaaccctcc ccctcctcca ggccgttcct
     6241 gatgaggtcc acggcggtga gcctgcaagc ccagatccat gccgccaggg gagccatcac
     6301 cctggccgtg gctgccaggt tcctgcccat gcgtatggag gcgttcctct gcctgagagc
     6361 cctgccatat tcgtgaagcc tcagggcata gacggggaac aggagggcac agagacgatc
     6421 gataagccgc ctcctcgtgg ccggtgaccc ctccaccaaa gagaggtcgt tggggagaaa
     6481 gatcagcgat ggcaccaggg gtctcagctg ggaagatcgc agggaggacc cacctttttt
     6541 cgcgacgatc cgggccccga tgcccacctt cacctccagg gaatcctccc cgtcgaaagc
     6601 tgccgagaga aaggtccctc taccgggctg ggtccagctc cggaggtccg cggtcctctc
     6661 gcccctgaga gagccccagc cggtcagcac gtgaaaagct tccagaatat tggtctttcc
     6721 ggagccgttg ggccccagaa gcagatgaag cccctccgcc cattccaggc gccggggctt
     6781 caggttcctg aatccttccc attgggtaga ggatattttc atgccgtctc ttctgaattc
     6841 tcctcctctg gaagggttat gggcatcaga acgtacatga agctgtcctc cctgggccgg
     6901 gaaagggtca tctggccatt gggcccgttg aaggagagat gggccaccgt tccgtggagg
     6961 gctttcaggc cttccagcag gtatcttacg ttgaaggcta tcttgagggg ttctccgtcg
     7021 cagtcgccgg ggatctcctc aaaggcctct cccacctcag gggccttggc ctgcatcttc
     7081 agggtgccac caggggacag catgaagacc accatccggc taaaatccct taccactacc
     7141 tcgacccttt caagggcctc tatgaaaagc tcacggtcaa tggtgagcca ggatgtcctg
     7201 tcagaagaaa ggaccttctc gtagggaggg aacctggatt ctacccttct tacggagaac
     7261 tccatggttc ccgtcctgaa gtagacctgg gaatcatcct tcagaaccct gatctcttca
     7321 tctccctcta ctgtggagag agatctctga agctccctga gcccggcaag ggggagaagt
     7381 acctggtcgg gttcaccatc caccgttacc gtaccctgcg aaagggagag tctccttcca
     7441 tcggtggata ctaccttaac cctctcttcg tcggtctgga ccaggccggc gctcagatac
     7501 tgggggaact cctcactggt ggatccggag aaggtccctt cctcaaggag acgcctcagg
     7561 tccatgcaga ctgctttgaa aaagggctcg gccttgtcag atgagggcaa cttggggaac
     7621 tcggagaccg gataggtgga aaaggagtag acactgctgc cggagatgac cgtcgccttg
     7681 cctttatcca cctcgatggt gaaagtgtca ccaggagcct tcttgaagaa ctctccaatg
     7741 ttcttcaccg ggaatatggc gcttccctcg gaaaggacct caactccgtc ggcaagacag
     7801 tttatggagg tcttcaggtc cgtagccttg agggataccc cctctgccga agcctcgcac
     7861 ttgacaccgg agaggaccga tatggtgctc ctgtgtcctg cgcttctctc tgccatactc
     7921 cagatcttca ggaattttgc cttgtccaca aggagcttca agatggtatc actcccttct
     7981 taatatctct tatataggaa ataaagaagt aggtctagta gtaagggtgt gattcatgtg
     8041 gagaacctga aaaactccat tggctcctgt cttttccctg tgcattcttt accctagatc
     8101 tgtggaggca cctccacaag agccacccct gatacgacag gaagggaaga tctgtggaaa
     8161 gaaatcacca ggaacacatc aggatcacaa cttttccctg aggttttcca caatgctctt
     8221 tatccttgaa tcttttctca gcgactcctc tatcttcttg caggcatgga tcacagttgt
     8281 gtgatccttc ctgttaaagg cgtagcctat ctgctgaagg gtggtccccg tcatgtcacg
     8341 gctgaggtac atagccacct gtcttgcaag ggctatctcc gatgtcctct tgttgccgga
     8401 gagtgaggag accgggatgc cgaaggattc tgcggtgacc tgctggatca gctctatggt
     8461 tatctgcccc ctcgatgatc tcctgatcag gtccttgagc catcgtgagg cgttatcgac
     8521 agtaacggac tcgttgttca ggtcggcaaa gaacattacg ctgttcaggg
//
LOCUS       DAOPKQ010000034         8514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_8813_length_8514_cov_4.803759, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000034 DAOPKQ010000000
VERSION     DAOPKQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8813_length_8514_cov_4.803759"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PK146_02180"
     CDS             <1..568
                     /locus_tag="PK146_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPE91078.1"
                     /translation="LEVIVPVQSEEDPGMDVLSDLKADVIVSAFTRVNRSLDIPTASN
                     YASHVLEAAGEFGIHPFMIASIIIRESTVRQNARSRYAYGLMQINWKAHRKGLTKAFS
                     TIRTLDDLLQPRNNIFAGTYIFSWYLRSSDGDMDKALSKYLGRTGKKYVSRVMSGYRD
                     MIKDLEERKDKYGSETVRSSSSVAFAVE"
     gene            615..1691
                     /locus_tag="PK146_02185"
     CDS             615..1691
                     /locus_tag="PK146_02185"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HPE91079.1"
                     /translation="MANSLPVLRIGKYSPRYPLIQGGMGVRISGPSLAGAVASAGGIG
                     TIASVGLAVASDVYDGRNYFEANQIVLKKDVEEARKSSGGGIIAVNAMVALTDYDLHV
                     RAACEGGADMIISGAGLPLRLPELTREFPHVALVPIVSSTKAASLIIRRWEKLYGRLP
                     DAFVVETPLYAGGHLGVTKMEHVMDEALSLESVIPGLVRYLDEEVGEDIPVIGAGGIW
                     DRGDMDKVFSLGARGVQMGTRFASTLEGDADIRFKQALIDAREEDVVIIKSPVGIPGR
                     ALRSPFVEKYLEGTVESKPCFANCLSHCSYRTDRQAFCIAQALVDAFRGNWEEGLFFC
                     GSNVARITRMETVEEIFRDLFGQE"
     gene            complement(1790..3085)
                     /locus_tag="PK146_02190"
     CDS             complement(1790..3085)
                     /locus_tag="PK146_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE91080.1"
                     /translation="MHYFRTRLVEFGVQTGRGFRQGLRALGSRNYRLFFMGQSISLIG
                     LWIQRVALSWLVYRITLSPLSLGFVDFIGQIPIFFLTLFTGVWLERWELRKLLLITQW
                     MAVAQALVLAALTLSGTILYWHIVILSTFLGVVNSFDMPARQAFVIQLVDRKDDLGNA
                     IALNSSLFNSARLIGPSIAGIAIAAVGEGICFLVNGLSFLATIAALRAIRVPPREPSI
                     RPKPGLSGVIDGLEYAFNFPPIKALLILLTLLSLFGLPYLVMLPVFAKEILGGGPKTL
                     GYLLASTGIGALAGTIFLALRKSPIGLGKVAAYATALFGVALSLFSMSRVQILSISLM
                     PLVGFGMVLSISSCNTLIQALVEEHMRGRVMSLYVMSIIGTGPVGSLLAGASAKLFGA
                     PLTLFIGGLVCVGASLIFFRNLPGLKALALPLLKEKGYS"
     gene            complement(3107..4942)
                     /gene="pepF"
                     /locus_tag="PK146_02195"
     CDS             complement(3107..4942)
                     /gene="pepF"
                     /locus_tag="PK146_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPE91081.1"
                     /translation="MKDPDHDSDHAGRGTIPERSDLEKTFKWALEDLFGDTTEWENAL
                     KEAGDLMDSLEAFRTKLSSSPSALLECLRLCDRIGELTSRVYAYAHMKSHEDTRDPAG
                     QAFAARATSLAVAASRSEAFVTPEILQIPEETLMEFLKREGDLEVYRFFLEEILRKRE
                     HVLSSREEELLAASGELAHAPENIFSMLTNADMTFPPVRDENGREVPFSEERFGTFLQ
                     SRDRRVREDAYNNLFGTYDRYRNTLTASLNSALRGSLFYSRARGFGSDLESALHGDDI
                     PLSVYSSLVDGVRESLDLLHRYVKLRGKVLDVSPVRMFDLYVPLVEEYRQDIPYRDAL
                     EMVRDGLKSLGGAYLAALDEGFSGGWIDVYENRGKRGGAYSWGAYPVHPYVLLNYNDR
                     IRDVFTLAHEMGHALHRFFTDREQPFVYSGHSIFIAEVASTTNEALLLDHLVRSASSR
                     EERMYLLNYRLEQIRTTVFRQTMFAEFEREVHGMTERGEAPTAETFCTLWRDLNEAYY
                     GPAVELDREIEIEWARIPHFYSPFYVYQYATGYSAATALSRSILEEGRTAADRFLRFL
                     ERGRSAPAIEVLRDAGVDMTSPEPVRTTLDLFGATLEELEMLLHI"
     gene            5178..5903
                     /locus_tag="PK146_02200"
     CDS             5178..5903
                     /locus_tag="PK146_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPE91082.1"
                     /translation="MLQGSFGWIALFAFLAATVNALGILVVFRYREQAEKLMPYLMCF
                     AAGVLISTPLMIALPAALERDPRSGFMALGGFLFMFFSNKVIRHYTHEEALAFGITAA
                     QGIAVHSFVDGIVYAVTFEVSVLVGVLSAGGLVAHEFAEGVITFLILVRGGVNRRAAM
                     VSAFLIASLTTPLGAFVAYPFIRPLSGGTLGLLLGFVSGVLIYVSASHLLPEARGHEE
                     RHSLLAFMAGVVLASFIVMTHGH"
     gene            5954..6310
                     /locus_tag="PK146_02205"
     CDS             5954..6310
                     /locus_tag="PK146_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91083.1"
                     /translation="MRLEKRSTIAVFLAVMVLFNLACQSFVMAANAWRVDVSEEAIGG
                     FGRFMEFARGLFRSAEEDGASEGSGSEKAFSTNPFVYIAAFWTEIVNAPEERSFWERI
                     IAFLEAVKVKGRGMIM"
     gene            6605..6925
                     /locus_tag="PK146_02210"
     CDS             6605..6925
                     /locus_tag="PK146_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPE91084.1"
                     /translation="MKEKFENVTLVSKANVYLDGKVTSRTFFLPSGERKTAGIFLPGE
                     YEFGTNEAEIMDVVQGSAEVLLPGETEWKKFGPGECFRIPSKSSFKLKIGEVLDYICS
                     YIKE"
     gene            complement(7443..8471)
                     /locus_tag="PK146_02215"
     CDS             complement(7443..8471)
                     /locus_tag="PK146_02215"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE91085.1"
                     /translation="MGAVSTGFLFTVGYGDWMRALALYALGSLGFSLSMIFYDSLLPH
                     IAPHGRIDEISSRGYAWGYLGGGALLAANIAAVVLLPGGLGARLSFLSVGLWWIAFTI
                     PLMVLVPEPVVEVNGAVEGVHVLTRGFGQLAETLRNIRRYRELLVFLVAFWLYNDGVG
                     TIMKMAAIYGAEKGIGMLHLVGALLATQFVGAPFSILFGKLSLRVGGKAAITIGLIWY
                     GVISFGAFFLERAWHFWVLAIAVGMVQGGVQAISRSTYGVMVPRARTGEFFALYDIFS
                     KFSGVAGPALFAVLTQLTGSTRMGIVSLILFFAGGIFLLRKVDVDKGMNEAGNDDGSP
                     KRSDRVRA"
BASE COUNT         1945 a         2267 c         2451 g         1851 t
ORIGIN      
        1 gcttgaggtg atcgttcctg ttcagtccga agaggatccc ggaatggatg tcctgtcgga
       61 tctaaaggcg gatgtcatcg tgtcggcttt tacaagggtc aacaggagtc tggacattcc
      121 tacggcatct aattacgcct cccatgttct tgaggctgcc ggggagttcg gtatccaccc
      181 cttcatgata gcctcgataa taatcaggga gtccacggtc agacagaatg cccgatcacg
      241 ctatgcctac gggctgatgc agatcaactg gaaggctcat cgcaagggtt tgacaaaagc
      301 tttcagcacc atcaggaccc tagatgatct gctgcagcct cgtaacaata tttttgcggg
      361 aacgtatatc ttctcctggt atctcagatc gagcgatggg gacatggaca aggccctctc
      421 gaaatacctc gggaggaccg ggaagaagta tgtctccagg gtcatgtcgg gttacaggga
      481 tatgataaag gatctggagg aacgcaagga caagtacggg agcgaaaccg taagaagttc
      541 ctctagtgtg gcctttgctg tagaatagac cggtcgttct ggtccagaat ccatcttaag
      601 agggagcggt gtaattggca aactctttac cggttctcag gataggaaag tattccccca
      661 ggtaccccct tatccaggga ggaatggggg tgcgcatctc cggcccgagc ctggccgggg
      721 ctgtcgccag tgcgggcggg atcggtacca ttgccagcgt aggacttgcc gttgcctccg
      781 atgtttacga cggcaggaac tacttcgaag ccaaccagat cgttctgaaa aaggacgttg
      841 aggaagccag aaagagctcc ggcgggggga tcatcgctgt taacgccatg gtcgccctca
      901 ccgactatga ccttcacgta agggctgcct gtgaaggcgg cgcagatatg atcatatccg
      961 gggcggggtt gcccctgaga ctcccggagc tgaccaggga attccctcac gtagcacttg
     1021 tccccatagt cagttccacc aaggcggcaa gcctgataat cagacgatgg gaaaagctct
     1081 acgggaggct tccggacgcc ttcgtggtcg agacgcctct ttacgcgggg gggcaccttg
     1141 gcgtgaccaa gatggaacat gtcatggacg aggcgctctc actggagtct gtcattccgg
     1201 ggctggtcag gtatctcgat gaagaagtgg gtgaggatat ccccgttata ggtgcggggg
     1261 ggatatggga ccgtggtgac atggacaagg tcttttccct gggcgctaga ggggttcaga
     1321 tgggcacccg gttcgcgtct acccttgaag gtgatgccga catcaggttc aaacaggccc
     1381 ttatcgacgc ccgtgaagag gacgtggtga tcattaagag ccccgtgggt atacccggcc
     1441 gggcgctcag gtctccattc gtcgagaaat acctggaagg taccgttgag agcaaaccct
     1501 gttttgccaa ctgcctctcc cactgttctt accggactga cagacaggcc ttctgtatag
     1561 cccaggccct ggtggacgcc ttccgcggca actgggagga ggggcttttc ttctgtggtt
     1621 ccaacgtagc caggataacc aggatggaga ccgttgagga gatcttcagg gatctcttcg
     1681 gtcaagagta gcgaccggag accttccacc ggatattgaa gggatcaggg ccgggtccgt
     1741 tctgtgaccc ggccctgatc cttggtcgac cttgaacgtc ggtcttctct cagctgtatc
     1801 ccttctcttt aaggaggggt aacgccaatg ccttcagacc cggaaggttt ctgaagaaaa
     1861 ttagcgatgc cccgacgcat accaggcctc cgatgaaaag ggttagtgga gccccgaaaa
     1921 gtttcgccga cgcacccgca agaagactcc ccacagggcc cgtacctata atggacatga
     1981 cgtagaggct catcacccgc cccctcatgt gttcctcaac tagagcctgt atcagggtgt
     2041 tgcatgaaga gatggagagg accattccga atcccaccag tggcataagt gaaatggaca
     2101 ggatctggac ccttgacatg gagaacagag acagcgctac cccgaaaaga gccgtagcgt
     2161 aagctgccac cttccccaga cctatggggc tcttgcgcag ggccagaaag atcgtccccg
     2221 ccagagcccc gatccctgtg gatgcaagga gataccccag ggtcttcggc ccgccaccga
     2281 gtatctcctt agcgaatacg gggagcatca ccaggtaagg cagcccgaag aggctcaaga
     2341 gcgtcaaaag gatcaacaag gccttgatgg gagggaagtt gaaggcgtac tcaaggccgt
     2401 caataactcc cgacagcccc ggcttgggcc ggatcgaggg ttccctcggc ggcaccctga
     2461 tcgccctgag tgcagcgata gtcgcaagga aggagaggcc gttgacaaga aagcatatcc
     2521 cctcaccgac ggccgctatg gctattcctg ctatggaggg tccaataagc ctggcggagt
     2581 tgaatagtga tgagttgagg gcgatggcgt tcccgagatc atccttcctg tcaacgagct
     2641 ggatcacgaa ggcctggcgg gccggcatat cgaaagagtt aaccactccc aggaaggtgc
     2701 tcaggattac aatatgccag tagagaatgg tgcccgagag ggtgagtgcg gcaaggacca
     2761 gggcctgggc gacggccatc cactgggtga tcaggaggag ttttctcagt tcccaccttt
     2821 caagccagac cccggtaaaa agagtgagga aaaatatggg gatctgtccg ataaaatcga
     2881 caaagcccag ggagaggggc gaaagggtga tacggtagac cagccagctc agggctaccc
     2941 tctggatcca gagccctatg agggatatgc tctggcccat gaagaagagc ctgtagttgc
     3001 gtgaaccaag ggctctcagt ccctgcctga aaccccttcc ggtctgaact ccgaattcca
     3061 caagacgggt cctgaagtaa tgcacggctc ttcctgatac ctcccgttat atatgaagga
     3121 gcatttcaag ctcttccagg gtggctccaa aaagatccag ggtagtcctg acaggctcag
     3181 ggctggtcat gtcgaccccg gcatcccgaa ggacctcgat ggcgggggct gaccttcctc
     3241 tttccaggaa acgaaggaac cggtcagcgg ctgttcttcc ctcttcgagg atacttcttg
     3301 aaagcgctgt cgccgccgag taacccgttg cgtactggta gacgtagaag ggtgagtaga
     3361 agtggggtat gcgggcccac tcgatctcta tctccctgtc gagttctacc gccgggccgt
     3421 agtaggcttc gttgagatcc ctccacaggg tgcagaatgt ctccgccgta ggggcctcgc
     3481 ccctttctgt cattccgtga acctcccgtt cgaattcggc gaacatggtc tggcggaaga
     3541 cagtcgtcct gatctgttca aggcgatagt tgaggaggta cattctttcc tcgcgactgg
     3601 aggctgaccg gacaaggtgg tccaggagaa gggcctcgtt ggtagtcgag gcaacctcgg
     3661 caatgaatat ggagtgaccg gagtatacaa agggttgttc cctgtcggta aagaacctgt
     3721 ggagggcatg ccccatctcg tgtgccaggg tgaagacatc ccttatccgg tcgttgtagt
     3781 tgaggaggac ataggggtga acagggtacg ctccccatga ataggctcca ccccttttcc
     3841 ccctgttctc gtaaacgtcg atccatcctc ctgaaaaacc ctcgtccagg gcggccaggt
     3901 aggctccacc cagggacttt agcccatctc tgaccatctc cagggcatca cggtaaggta
     3961 tatcctggcg gtactcttcc accaggggta cgtaaaggtc gaacatcctt accggggaaa
     4021 catccagcac cttcccccgg agctttacat agcgatggag cagatcgagg ctttcacgga
     4081 ccccgtccac aagggaggag tagaccgaaa gtggaatatc gtccccgtgg agggcggact
     4141 cgagatccga gccgaatccc cttgcccttg aatagaatag cgaaccccgc agggcagagt
     4201 taagggatgc cgtgagggta ttcctgtacc ggtcgtaagt tccgaagagg ttgttgtagg
     4261 cgtcctctct gaccctacgg tcacggctct gcaggaaggt tccgaaacgt tcctccgaga
     4321 aagggacctc cctcccattc tcatcgcgta caggggggaa ggtcatgtcc gcattggtga
     4381 gcatggagaa aatgttctcc ggagcgtgag caagctctcc tgacgcggcc agcagttcct
     4441 cctccctcga agacaacaca tgttcccgct ttcgaagtat ctcttccagg aagaaacgat
     4501 agacctcaag atccccctct cgtttcagga attccatgag ggtttcttcg gggatctgga
     4561 ggatctccgg tgtgacgaag gcttcagaac gcgatgccgc taccgccagg gaggttgctc
     4621 ttgctgcgaa ggcctggcct gccggatctc tggtatcttc atggctcttc atgtgggcgt
     4681 aggcgtacac cctggacgtc agctcgccga tcctgtcaca gagcctgagg cattccagca
     4741 gtgccgaggg cgaggaagag agcttcgtcc tgaaggcctc aagggaatcc atgagatccc
     4801 ctgcctcttt cagggcgttc tcccactcgg ttgtgtctcc aaaaaggtct tcaagggccc
     4861 atttgaaggt tttctccaga tctgaccttt cgggtattgt acctctgcct gcgtggtcgc
     4921 tgtcatgatc agggtctttc atcgtcaggt ctccttctga tcctgccctg aagggtgagg
     4981 gggatccatg atgggtgatc ttccagataa cctagatgct agcataagaa agaacaaact
     5041 gcctgacctg tgatggaagt gagaagaaag agcctcccgg gataactccg ctgtcagaaa
     5101 gagagaatta cctgtggggg ggtaataggg tctacaatag atcatgacca tgccgcgaca
     5161 atcaaggggg aaggtcggtg ctgcagggat ctttcggatg gatcgctctt tttgcttttc
     5221 tggccgctac tgttaacgcc ctgggcatcc tggtggtctt caggtacagg gaacaggccg
     5281 aaaagctgat gccatacctg atgtgtttcg cagcgggggt ccttatatcc acacccctga
     5341 tgatagccct ccccgcagct ctggagaggg atccccgctc aggattcatg gctcttggcg
     5401 ggtttttgtt catgttcttc tccaacaagg ttatccggca ttacactcac gaggaggcac
     5461 tggccttcgg gataactgcc gctcagggga tagcggtcca ctcgttcgtg gacggcatcg
     5521 tctatgccgt caccttcgag gtgagcgttc tggtgggggt cctctctgca ggaggactcg
     5581 tggcacacga gttcgcggaa ggagttatca ccttcctgat cctcgtaagg gggggagtga
     5641 acagaagagc agccatggtc tccgctttcc tgatagcatc ccttaccaca cctctcgggg
     5701 cctttgtggc ctaccccttt ataagacccc tgagtggtgg caccctgggg ttgctgctcg
     5761 gattcgtgtc tggcgtgctc atatatgtct ctgcctcgca cctgctcccg gaggcaaggg
     5821 ggcatgaaga acgccactca cttttggctt tcatggcagg agtggtcctt gcatccttta
     5881 ttgtaatgac ccatggacat tgaacggcag ggcagggtcc cttcctggat gttaacttta
     5941 tggggggaga tggatgaggc tggagaagcg ctcaacgata gctgtttttc tggcagtcat
     6001 ggtccttttc aatcttgcct gccagagttt cgttatggcg gcaaatgcct ggagggtgga
     6061 tgtctctgaa gaggccatcg ggggcttcgg aaggtttatg gagttcgcca gaggtctctt
     6121 ccgatctgcc gaagaggatg gggcttcgga agggtcggga agcgaaaagg ccttttcgac
     6181 gaaccccttc gtctatatcg cggccttctg gacagagatt gtgaacgcgc cagaagaaag
     6241 gtctttttgg gaaaggatca tagcctttct tgaggcggta aaggtgaagg gacggggaat
     6301 gatcatgtaa ctcgtgactc gtgacgggtg aaatccgtaa accgtgaatc gtgaaccgaa
     6361 aaacagatct tgcaccacag agaaaacaag gtcactattg ggcagagtct ggatcgttga
     6421 tcttttcttg ccttttccgt tcaccgttca ctgtttgccg ttcacgcagt ttacccttcg
     6481 agcattgacc ttggttcaaa cattacgatt aacgactcac ggttcacgat tgacggatca
     6541 agatttccga ttcacagatc acggtcttgt aaaaacggtc cactaaacga aaggaggtct
     6601 cgcaatgaag gaaaagttcg agaacgttac tcttgtaagc aaggccaatg tctatcttga
     6661 cggcaaggtg accagcagga ccttcttcct cccctcggga gagaggaaga cggcgggcat
     6721 cttcctgccc ggagagtacg agttcggaac taacgaggct gaaataatgg atgtcgttca
     6781 gggtagtgcg gaggtactgc tccccggaga gacggaatgg aaaaagttcg gtcccggtga
     6841 gtgcttcagg attccttcaa agagctcctt taaactaaag atcggggaag ttctcgatta
     6901 tatctgctca tacatcaagg agtgacaaac ccttcgttgc tcctggggga taagcccgtc
     6961 acccccagga gtctatcggt tggagaagta gatgaagaac atgaccccca tgaaaaaacc
     7021 taccgacaag gctttcacga tccttgaagt atcccttctt tccggtcttt tcgggtcgaa
     7081 atatccggac ggggatcctg ttctttcttt tttacgtctc ttccctgtaa atttgtccgc
     7141 ataggaataa gtcaggaaac ccgcacccag gcccataaaa acggcgcctg ccagtggctg
     7201 tagcaaagaa ccgtccatcg gtctacccct tcccgggagg agtctttctc cttccaatat
     7261 tgcagaggct atcctttcag ggaagaccgt gtcaagggcg acctgtcggg atgccttatt
     7321 cgggaggatc ccctgggcga agatgtgatg gaaggtgaga agaaaaaccc ttgaatcata
     7381 aatcgtgatt cgtggatcgt gaaccgaaaa ggctggaaaa gaaagcggca ggaaggacag
     7441 gatcaagcgc gtaccctgtc gctgcgcttg gggctcccgt catcattccc tgcctcgttc
     7501 atccccttgt cgacatccac cttgcggagc aggaagatcc ctcctgcgaa gaaaaggatc
     7561 agggagacta tccccatacg ggtggagccg gtgagttgcg tcagaaccgc aaaaagggca
     7621 ggtccggcca ctcccgagaa tttgctgaat atgtcgtaaa gggcgaagaa ctcccccgtc
     7681 cttgcccggg ggaccattac gccgtaagtg ctccggctga tggcctggac acctccctgg
     7741 accattccta cagcaatggc caggacccag aagtgccatg ccctttccag gaagaaggcg
     7801 ccgaaagaaa tgacaccgta ccatatcagc cctatggtta ttgccgcctt gccccccacc
     7861 cttagggaga gtttcccgaa gaggatggag aagggggctc cgacgaactg ggtggcgaga
     7921 agggccccaa cgaggtgcag catccctata cctttctcgg caccgtagat ggcggccatc
     7981 ttcattatcg tgccgacgcc gtcgttataa agccagaaag cgaccaggaa aaccagtaat
     8041 tcccggtagc gcctgatgtt cctcagggtc tccgccagtt gaccgaaccc ccgggtaagt
     8101 acgtgcaccc cttcaactgc accgttgacc tcgacaaccg gctccgggac aaggaccatc
     8161 aagggtatcg tgaaggcgat ccaccacagc ccgacggaca ggaaagacaa tcttgctcca
     8221 aggcctcccg ggagaagaac caccgctgca atgttggcgg caagtagtgc gcctccccca
     8281 agatatcccc atgcgtaccc tctggacgat atctcgtcga tcctgccatg gggggcgatg
     8341 tgggggagaa gtgagtcgta gaagatcatg gacagggaaa accccaggct tccaagggcg
     8401 taaagagcca gtgccctcat ccagtcgccg tatccgacgg tgaaaaggaa acccgtagaa
     8461 actgcgccca tgcgagtcct ccgaaacaaa ggttagcaca ggttcgtaca tacg
//
LOCUS       DAOPKQ010000035         8470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_8887_length_8470_cov_4.694949, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000035 DAOPKQ010000000
VERSION     DAOPKQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8887_length_8470_cov_4.694949"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PK146_02220"
     CDS             complement(<1..518)
                     /locus_tag="PK146_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPE91086.1"
                     /translation="MPKMNYGNDIGHSLRFMMEQDPAFRTVAYFSMEVGLKASIPTYS
                     GGLGVLAGDILKSAADLGVPIVGVTLLYRKGYFRQSFEDCLQKALPVEWDPEKQLALL
                     PHEVTVMIEDRVVKVRAWCLELQGRTGFTVPIYFLDTDVEGNSPADRELTWYLYGGDE
                     RYRLCEEIILGSG"
     gene            complement(587..2083)
                     /gene="malQ"
                     /locus_tag="PK146_02225"
     CDS             complement(587..2083)
                     /gene="malQ"
                     /locus_tag="PK146_02225"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPE91087.1"
                     /translation="MGRSCGVLLHISSLPSRWGIGDLGRGADLFLDFLCSSRQSLWQI
                     LPLSPTDPGQGNSPYSSFSAFGGNPLFISPELLVKDGLLELSDLDSHEPLSMKACDHA
                     GSARIKRSLLQRAYKRFASSEDSEYYLFCEKNGHWLDDLALFLSLKRHLGGVPWCEWP
                     APLRDREEKALAEAREQLADKIGYARFLQYVFERQWGRLKRVCREKGISVIGDVPVYV
                     SHDSADVWAHRELFDLDPTGRAKKVAGVPPDYFSESGQRWGNPVYRWDMLEKEGFWWW
                     IRRLSNALSLFDMVRIDHFRGLVKFWSIPSSERSAIDGEWVEASPDAFFLRVKENFPA
                     MPFIAEDLGYITPDVKDYIRRLGIPKTLVLQFAFGDDLEKSPYAPANHIENACVYTGT
                     HDNNTTRGWFESEATPLMRSQLNEIAGHKVTPETVSMDMISIAMASKASMTVYPMQDV
                     LGLGQEARLNTPGKPSGNWLWRLEERQLEDAPVEKLAEMTARFGREAR"
     gene            2201..2914
                     /locus_tag="PK146_02230"
     CDS             2201..2914
                     /locus_tag="PK146_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91088.1"
                     /translation="MQEKGPTWRLDRVFKTYDMGEVKVEALRKSSLSIPEGELVVILG
                     PSGSGKSTLLNLLGGMDVPSGGDVWFRDENLSAASDRQLTLYRRNVVGFVFQFFNLIP
                     DLTARENVDIAAELVDSPFPTEDVLDGVGMSDRIDHFPSQMSGGEQQRVAIARALVKN
                     PLLLLCDEPTGSLDASTGRLILAQLEEINRKFGTTVVIVTHNASIADMARRVVRMSSG
                     RIVEETYNEEPLPPERISW"
     gene            2914..5292
                     /locus_tag="PK146_02235"
     CDS             2914..5292
                     /locus_tag="PK146_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91089.1"
                     /translation="MPVLARKLLRTIGSTLGQFIASAVVIATGVMAFVTMTAMSDNLI
                     RSRDSFYRETDFADHFFHVVRAPEGVLSRVEAVPGVLRASVRIQKDVPVINNEDERTT
                     IRLLGYPLPDRQELNRVKVLSGRMAEKYPEGGAVEILVNRQFAEAHGFRQGDTIDIAA
                     EGRRKTLTITGTAAGPDFVYAVKDAASMLEDPSSFGVAVLPQNQLQQILGEKGSVNQV
                     LVRFIPGADRVGVAREIRNILEPYGSLADYEQKDQISEAILRGELNQLKAFARFLPTI
                     FLGIAVLMQFVFIGRMVQVQRGQIGIMKAVGYGNVSLLALYGGYALSAASAGAVAGTG
                     LGYFFAGLMTKLYMMFFNLPRLEEALHFRAAGLVFGTTLVVGTLAGVWAARGVLAVSP
                     AEAMRPATPRSFRPSMIENIPGLMEHLGLSWRMSLRSMFRNRFRSTVTILGLLLAVGM
                     IVISIFARDSMFDLMERHFEKEWRFDFLVRVVNPVKEKDLLSISNLPGVREAEPILEL
                     PVRIHYKGRYREDVLFAMHTGSGLGRVIARDGHELDFPEEGLFLDWYTGRILGAGTGD
                     EVEVETILGLGPPRKSMLRVAGISRKAFGSASSVSLDTANRLLNEQGIASGIVFNTQD
                     EFKESIEYALGDMINVISVTSREKEVRFLEKNMKYLYYSVGIMIFFSVLLGFAIVFNA
                     SSLSFSERRRELASLRVLGFSAREVSSLLWKENLLLLAVGAVLGLPFGRLMAEAYAIG
                     VSTDLFTFEAVVYPTTYAISVLGGGLFILVSWGLAARSVGRLQPVETLKAND"
     gene            5306..6472
                     /locus_tag="PK146_02240"
     CDS             5306..6472
                     /locus_tag="PK146_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE91090.1"
                     /translation="MKKRRLKILATSGIIILLAVTLFFLLRPEKMAAVRVVRGEISSG
                     VEEDGYLQPLEDRNFYATQTARITELPVEAGDEVSQGQLLIGMTNPDLEAILAETRAY
                     WRQAEKSEIGYAAAAASLRLLLADENRNMERYRKLYEAGALSMTEMEQVSLRVERYTK
                     DLAEAEARLGSASALSRGLRQQVLELEKKQAELTLKSPIDGYVLDLPVKKEQVVMAGE
                     LLVALGIEDEMEIRSDVLSDSLGGVKVGSRVRITAPILGSLTLEGHVKKIYPLAEEKV
                     SPLGVAQRRVPVIIAFPMSEVLKPGYEVRVFIETSRREDVLLLPVESVRTEDGIRKVF
                     QLQGGKIHATEVRIGITDRRMVEILEGLEEGDVVVRDASLDLSDGQRARAAEDI"
     gene            complement(6478..6894)
                     /locus_tag="PK146_02245"
     CDS             complement(6478..6894)
                     /locus_tag="PK146_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91091.1"
                     /translation="MIKGCLAVFLSASLFFMTVSAGFACSEYDPLVGGLEREGVIEAL
                     QGELLESFGIEAEFVFNWFGIEDVWAWVESEPRSPDGLNRYEPFLALLKKEGENWAVT
                     EVPPLEDGSPPVDDAYFRSLMERYPGLPEGIFPWER"
     gene            complement(6964..7842)
                     /locus_tag="PK146_02250"
     CDS             complement(6964..7842)
                     /locus_tag="PK146_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE91092.1"
                     /translation="MQFRGELDSARIVTVLDDYAGYETSFLAQHGIAILLEASSGGVC
                     KRVLLDAGQSALPILHNLEIMDIDPGSIDAIVVSHCHYDHTEGLVEVLRAVGREGIPV
                     IAHPTMFRPNYIFKPFIRNIGVTGKNGKVAIEEAGGLLVLVDEPFEIMPGVIVTGEVP
                     RRRAFEKQGIGTYNIEGGKVVPDEIRDDLSLTVKVKGKGLAVVSGCSHAGIVNIIDWC
                     REIAAEPRVDLVIGGFHLIEASGERIRETAQALKQLNVGRIVTGHCTGLEALCEFSRI
                     LGDRFEQLHVGTTIQL"
     gene            complement(7864..>8470)
                     /locus_tag="PK146_02255"
     CDS             complement(7864..>8470)
                     /locus_tag="PK146_02255"
                     /inference="COORDINATES: protein motif:HMM:NF017347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HPE91093.1"
                     /translation="GPAVALGLLVTSTGITYLGATASTIFPDAGIGVYTVMIAEGVLG
                     AVGKAVFALLLAFACFTTSVGLTSTAGDVFQEMSGGKLQYKTIVAVSSLVGFGIGTVG
                     LGKIVGYTVPWLMLVYPAIIVLLVGSLFDFSKYKPALQGAIITAVILSIGDFLGGMGM
                     ADNPFSAMTAKLPLGGQGLGWLVPALVVGALAMLLPGRSKK"
BASE COUNT         1894 a         2410 c         2415 g         1751 t
ORIGIN      
        1 cctgagccca ggatgatctc ctcgcagagc ctgtaccttt cgtccccccc gtagagatac
       61 catgtgagct ccctgtccgc gggagagttg ccctccacat cggtatcaag gaaatatatt
      121 gggacggtga accctgtcct gccctggagt tcaagacacc atgccctgac ctttactacg
      181 cggtcctcta tcattacggt cacttcgtgg ggcaggaggg cgagttgctt ttccggatcc
      241 cactccaccg gcaatgcctt ctggagacag tcctcgaagg actgcctgaa gtatcccttt
      301 cggtaaagta gtgttactcc gacaatggga acacccaggt cggcagcact cttcagaatg
      361 tccccggcaa gtacacccag gccaccggag taggttggta tggatgcctt caggcccacc
      421 tccatcgaga agtacgccac tgttcggaaa gccgggtcct gttccatcat gaagcggagc
      481 gaatgtccaa tgtcgttgcc gtagttcatc ttcggcatta ctacactacc tccctgcctt
      541 ttctctttcc cctgttattt attatctcat ctggcaagaa aatgccctaa cgggcctctc
      601 tcccaaatcg ggccgtcatt tcggccagtt tttctacagg agcgtcctcc agctgcctct
      661 cttccagcct ccacagccag ttccccgaag gctttcccgg ggtatttagc cgagcctcct
      721 ggccaagacc aagaacatcc tgcatcgggt ataccgtcat ggaagccttc gaagccatag
      781 ctatggatat catatccatc gagaccgttt cgggagttac cttgtgcccg gcgatctcat
      841 tcagttggct tctcatcaac ggtgtagctt cggactcgaa ccaaccccgg gtcgtattgt
      901 tgtcgtgggt gccggtatag acacaggcgt tctcaatgtg attcgccgga gcgtaaggac
      961 tcttttcgag atcatcaccg aaggcaaatt gcaggaccag agtctttggg atccctaatc
     1021 ggcgaatgta atccttgaca tcaggtgtaa tgtatcccag atcctcggca ataaagggca
     1081 tggctgggaa attctccttc accctcagaa aaaaagcatc cggtgaagct tctacccatt
     1141 ctccatctat agcagacctt tcagaggaag ggatggacca gaacttgacc aggccccgga
     1201 agtgatcgat cctgaccatg tcaaacaggg aaagggcgtt ggaaagcctc cggatccacc
     1261 accaaaagcc ctctttttcc agcatatccc accggtagac tgggttgccc caccgctgac
     1321 cactttccga gaagtagtcc ggagggaccc ctgctacttt ttttgctctg ccggtggggt
     1381 caagatcgaa cagttcgcgg tgggcccaaa cgtctgcact gtcgtggctg acgtagaccg
     1441 ggacgtcccc tatgacagaa atgcctttct cccggcagac tcttttaagc cgtccccact
     1501 gcctttcaaa aacatactga aggaaacggg cataccctat cttgtcggcc agttgctccc
     1561 tggcctcggc gagggccttc tcctccctgt ccctcagggg tgcgggccat tcgcaccatg
     1621 gcactccgcc aaggtgtctt ttaagggata agaaaagggc aagatcatcg agccagtgac
     1681 cgttcttctc acagaacagg tagtattcgc tgtcctcaga ggatgcgaac cttttatagg
     1741 ccctctgtaa gaggctcctc tttatccggg cagagccggc atgatcgcag gccttcatgg
     1801 acaaaggttc atgtgaatcg agatctgaca gctcgagcaa tccatccttg actaggagtt
     1861 ccgggctgat gaaaagcgga ttccccccaa aggcagagaa actgctgtag ggggagttgc
     1921 cctggccagg atccgtaggg gaaaggggga gtatctgcca cagggactgc cgtgaagagc
     1981 agaggaagtc caggaacaga tcggcccctc tgcccaaatc acctatgccc caccgggagg
     2041 gcagggagga tatgtggagg agaacaccgc agcttcttcc catgctaccc attgaagttt
     2101 gtctgacgat cattgtctga ttttacagta aaaccgacgc ccggtttgac ctttttagac
     2161 gacagaacgt atacttcaca gcatccgggg ggtatttttc atgcaggaaa aggggccgac
     2221 ctggaggctc gacagggttt tcaagaccta tgacatgggt gaagtaaaag tggaggctct
     2281 caggaaatca tcgctctcga tccccgaggg ggaactggtt gtcatcctgg gccccagcgg
     2341 atccggcaag agcacactcc tcaacctgct gggcgggatg gacgtcccct ctggcgggga
     2401 cgtctggttc agggatgaga acctctccgc cgccagtgac aggcagctta ccctctaccg
     2461 acgtaacgtg gtgggtttcg tattccagtt cttcaacctg atccccgatc tgacagccag
     2521 ggagaacgtc gacatcgccg cggaactcgt tgactcccct ttccccaccg aagacgtgct
     2581 tgacggtgta ggcatgtccg acaggattga ccacttcccc tcccagatga gcggcggcga
     2641 acagcagcgc gtcgctatag ccagggccct tgtgaagaac ccgctgctat tgctctgcga
     2701 cgagccgaca ggctctctcg acgcgtccac aggaaggctg atccttgcac agctggaaga
     2761 gataaacagg aagttcggga ccaccgtggt gatagttacc cacaacgctt ccatagctga
     2821 tatggcccgc cgggttgtca ggatgagcag cggcaggatc gttgaggaaa catacaacga
     2881 ggaaccactt ccgccggaga ggatctcctg gtgatgccgg tcctggccag aaaacttctc
     2941 agaaccatag gctcgaccct gggtcagttc atcgcatcgg cagtggtcat cgccacggga
     3001 gtaatggctt tcgtgacaat gaccgccatg tctgacaacc tgataaggtc cagggattcc
     3061 ttctaccggg agacggactt tgcagatcac ttcttccacg tcgtcagggc tcccgagggg
     3121 gtgctttcgc gcgtggaggc agtcccaggg gtcctccggg cctctgtgag gatccagaag
     3181 gacgtgccgg taataaataa cgaggatgaa aggaccacga tccgactcct tggttatccg
     3241 ctgcccgacc gccaggaact taacagggtc aaagtcctct cgggcaggat ggccgagaag
     3301 tatcccgagg gcggggcggt ggagatcctg gtgaacaggc agttcgccga ggcccatggt
     3361 ttcaggcagg gggatactat agacatagca gccgagggga gaagaaagac ccttaccata
     3421 acaggaacgg cggcgggtcc cgatttcgtc tacgcagtga aggacgccgc gtccatgctt
     3481 gaagacccat cctccttcgg tgtggcagtc ctgccccaga accagttaca gcagatattg
     3541 ggcgagaaag gttctgtcaa ccaggtactg gtccgtttca tccccggagc ggacagagtt
     3601 ggagtagcaa gggagatcag gaacatcctt gaaccctatg gcagcctggc cgactacgaa
     3661 cagaaggacc agatcagcga ggctattctc agaggggagc tcaaccagct taaggctttc
     3721 gcaaggttcc tccccacgat attcctgggt atagcggttc tgatgcagtt cgtctttatc
     3781 gggaggatgg tacaggtcca gaggggccag ataggaataa tgaaggcagt ggggtacggc
     3841 aacgtttcac ttctggccct ttacggaggg tacgcccttt cggccgcctc tgcaggtgcg
     3901 gtggccggaa ccggtcttgg ctatttcttt gcaggtctga tgaccaagct atacatgatg
     3961 ttcttcaacc tgccacgcct ggaggaagct ctccacttca gggcggcggg actggttttc
     4021 gggacaaccc tcgtcgtagg gaccctggcc ggtgtctggg ccgcgagggg agtgctggcg
     4081 gtaagccccg cagaggccat gagaccggcc actccacgat ccttccgccc gagtatgatc
     4141 gagaatatcc ctggactaat ggaacacctg ggactgagct ggcgtatgag cctgcgctcc
     4201 atgttcagga acagattccg gtcgaccgtc acgatcctgg ggctcctcct tgccgtaggg
     4261 atgatagtta tctcaatttt tgccagggat tccatgttcg acctcatgga gagacacttc
     4321 gaaaaggagt ggcgattcga cttcctggtc agagtggtca accccgtgaa agagaaggat
     4381 ctgctgtcca tatcgaacct tccgggggta agggaggccg aacccatatt agagctcccg
     4441 gtaaggatcc actacaaggg gcggtacagg gaggatgtcc tcttcgccat gcacacggga
     4501 tccggccttg gcagggttat cgcccgggat gggcatgaac tggattttcc cgaggagggc
     4561 ctgtttttgg actggtacac cgggagaatt ctgggcgcgg ggacgggtga cgaagtggag
     4621 gttgagacca tccttgggct gggcccaccc agaaagagca tgctgagggt ggcgggcatc
     4681 agccgcaagg ccttcgggag cgcatccagc gtaagccttg atactgccaa ccgtctgctg
     4741 aacgaacagg ggatcgcctc gggcattgtc ttcaacaccc aggacgagtt caaagaaagc
     4801 atcgaatatg cactgggaga catgatcaac gtaatctccg tgaccagcag ggagaaagag
     4861 gtacggttcc tcgaaaagaa catgaaatac ctttactatt ctgtcgggat aatgatcttc
     4921 ttctccgtgc tgctgggctt tgccatagtc ttcaacgcgt cttccctctc cttctccgaa
     4981 cggcggaggg aactggcaag cctgagagtg ctgggattct cggcgagaga ggtctcatcg
     5041 ctgctatgga aggagaacct tctccttctt gccgtggggg ccgtgctggg actccccttt
     5101 ggcaggctca tggccgaggc ttacgcgatc ggagtcagca ccgacctctt caccttcgag
     5161 gcggtggtct accccactac ctacgccatc tccgtcctgg gcggagggct gttcatcctc
     5221 gtctcctggg gtcttgcggc aagaagcgtc ggcagactcc agccggtgga gactctgaag
     5281 gctaacgact gaggaggtat ggaccatgaa aaagagaaga ctgaagatct tagcgacttc
     5341 cgggatcatt atcctgctgg ctgtcacact cttcttcctc ctcaggcccg agaagatggc
     5401 tgcggtcagg gtagtcagag gggagatatc aagcggggtc gaggaggacg gctaccttca
     5461 gcctttggag gacaggaact tctacgcaac ccagaccgct aggataactg aactccccgt
     5521 agaggcggga gacgaggtct cgcagggcca gttgctcata ggcatgacaa accctgatct
     5581 ggaggcgatc ctcgccgaga cgagggcata ctggagacag gccgagaaga gcgagatcgg
     5641 ctatgcagct gcggcagcga gccttcgcct tcttctggcg gatgagaatc gaaacatgga
     5701 gcgctacaga aaactctacg aggcaggagc cctttcaatg accgaaatgg agcaggtcag
     5761 cctgagagta gagcgataca ccaaggacct tgccgaggcc gaggccaggc tggggtcggc
     5821 ctcggcactg agcagaggac ttcgacaaca ggtcctggaa ctggagaaga aacaggccga
     5881 attgacactg aaaagcccga tcgacggcta tgtcctcgac ctcccggtca agaaggagca
     5941 ggtggtcatg gcaggagagc ttctggtcgc tctcgggatt gaggacgaaa tggagatacg
     6001 ctcagacgtt ctcagcgact cccttggagg agtgaaggtc gggagcaggg tgaggataac
     6061 agcccccatt ctgggcagcc ttacactgga gggtcatgtc aagaagatct atcccctggc
     6121 ggaggagaag gtctcccctc tcggggtggc ccagagaagg gtgccggtca ttatcgcttt
     6181 ccccatgtcg gaggttttga aacccggcta cgaggtcagg gtcttcatag agacgtctcg
     6241 cagggaggac gtgctgctgc tgccggtgga gtctgttcgt accgaagacg ggatcaggaa
     6301 ggtcttccaa ctccagggcg ggaagatcca tgcaaccgag gtcaggattg gcatcaccga
     6361 ccgcaggatg gtggagatac tcgaggggct ggaagagggc gacgtggtcg ttcgggacgc
     6421 aagccttgac ctcagcgatg gtcagagggc cagagccgcc gaagacattt aggccctcta
     6481 tctctcccag gggaagatcc cttccggcag gcctggatat ctctccatca ggctcctgaa
     6541 atatgcatca tcaacgggag gggagccatc ctccagaggg gggacctcag tgacggccca
     6601 gttttcgccc tccttcttca agagggcaag gaagggctcg tacctgttca gtccgtccgg
     6661 agaccgtggt tcgctctcca cccacgccca aacatcctcg ataccgaacc agttgaagac
     6721 aaattcggcc tctatgccga aactttccag aagttccccc tgaagggcct caatgacccc
     6781 ttcccgttcc aggcccccca ccagggggtc gtactcactg caggcaaacc ccgctgaaac
     6841 agtcatgaaa aaaaggcttg cagacaaaaa gacagccagg catcctttga tcatctcaga
     6901 ctcttccttt ccggaagaag ggcggtaccc cttaagggcg ccgccctgga aaatttcctg
     6961 gatctacagc tggatcgtgg tccccacgtg cagctgttcg aagcgatccc cgaggatcct
     7021 ggagaattcg cagagggcct ccagacctgt gcagtggccc gtcacgatcc ttccgacgtt
     7081 cagctgcttc aaagcctgcg cagtctccct gatcctctcc ccagaggcct cgataaggtg
     7141 gaagccacct atcaccaggt ccactctggg ctcggcggcg atctccctgc accagtcgat
     7201 tatgttgact attccggcgt ggctgcagcc ggagacaacg gccaacccct tccccttgac
     7261 cttgacggtc agggagagat cgtctcgtat ctcgtcggga acgaccttgc ccccctcgat
     7321 gttgtaggtc cctattccct gcttctcaaa ggcccttctc cgggggacct cccctgtaac
     7381 aatcactccc ggcattatct cgaagggttc gtcgacgagg accagaagcc ccccggcctc
     7441 ctcgatggcg accttaccgt tctttccggt gacgccaata ttccggataa agggtttaaa
     7501 gatgtagttg gggcggaaca tcgtcgggtg agcaatgacg ggaatgccct cccggcctac
     7561 ggccctcagg acctcaacaa ggccctcagt gtggtcgtaa tgacagtggg agacgacaat
     7621 ggcatcgatc gagccgggat cgatgtccat tatctcaagg ttatgcagta tcggcagcgc
     7681 cgactgcccc gcatccagaa gtaccctttt gcacacccct ccggaagaag cctccaggag
     7741 tatggctata ccgtgctggg ccagaaagga tgtctcgtac ccggcgtagt cgtcaaggac
     7801 ggtcactatc cgggctgaat caagctctcc cctgaactgc atattatcag acctcctagg
     7861 gtgttacttt tttgaccttc ctggcaggag cattgccaaa gcaccgacca caagcgccgg
     7921 cacaagccaa cccaacccct ggccgccaag gggaagcttc gccgtcatgg cactgaaagg
     7981 attgtcagcc atgcccatgc caccgagaaa atcaccgatg ctcaagatga ccgctgtaat
     8041 aatggcaccc tggagggccg gcttgtactt gctgaagtca aaaaggcttc ccaccagcag
     8101 aacaataata gccggataga ccagcataag ccagggtacg gtgtaaccta cgatcttgcc
     8161 gagccctacc gtcccgatgc caaagccgac gaggctcgag acagcaacaa tggtcttgta
     8221 ctgcagtttg cccccggaca tctcctggaa gacatcacct gccgtggaag tcagaccgac
     8281 ggaggtggtg aagcaggcga atgcaagaag cagcgcgaaa acggcctttc ctacagcccc
     8341 cagtactccc tctgcgatca tgacggtgta gacccctatt cccgcatcgg ggaaaatggt
     8401 gctggccgtg gcgccgaggt aggtgatgcc cgtggacgtg accaaaagtc ccagtgcaac
     8461 tgcggggcca
//
LOCUS       DAOPKQ010000036         8467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_8891_length_8467_cov_4.292083, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000036 DAOPKQ010000000
VERSION     DAOPKQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8891_length_8467_cov_4.292083"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PK146_02260"
     CDS             complement(<1..436)
                     /locus_tag="PK146_02260"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPE91094.1"
                     /translation="MIRHVRLQVTGVVQGIGFRPFCSRLSGEIGLGGSVRNTTAGVVI
                     DLYGDPEGIDSYIERLQTDCPTLGHIQSITIVLDERIDDIRKRPFEILDSHGSGGEYA
                     LIPPDIATCSDCLAELRDPGNRRFRYPFINCTNCGPRFTIITD"
     gene            554..3106
                     /locus_tag="PK146_02265"
     CDS             554..3106
                     /locus_tag="PK146_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPE91095.1"
                     /translation="MDLTKAHALDAKEILAALDVDPEKGLDDLRVEELIERYGPNQIN
                     PEEPEPWWKSLLRQFMTPMIYLLTGASLISLVMKETLDAFAILVVILINGGIGFVTEF
                     RAEKALMALKAMISPTAVALRGGVIRTVSAASLVPGDIILLEAGDVVPADARVFEQYN
                     LGVDEAALTGESLPVDKTGEILPVGTELADRKNCLFSGTAVVRGSGRAVVFGTGMGTE
                     IGRISSMLSTVSKSATPLEERLARLSGLLIRMVIGIAILVTGLGVLQGRGIVRMIETG
                     IALAVAAVPEGLPFVATMTLAIGVHRMAKRNALVRNLASVETLGSTTVICTDKTGTLT
                     MNDMAVRSMVPAHPECEDLLLRVSVLCNKAHLEEDSQVGDPMEVALLRSALSRGIDIG
                     ATRRATPIIGEEPFDSQTMRMITWHRDGVAAKGAPERILQMCRKVWSKNGLVTFSEEY
                     RREWMERIEAMAAQGMRTLAFAWGDDEGDLSFLGVAGIFDPPRPEVRDSVRSCIDAGI
                     HVIMVTGDHLVTARSIAAEVGILGQGGSKALSGSELAEMEEGAIASQAREIAVVARVA
                     PEHKLRIVRSLQASGEVVAMTGDGVNDAVALKQADVGIAMGIQGTEVSKEASDIILQD
                     DRFSTIVTAIGEGRKIFDNIRKSVLFLLCCNLSEVITVLGGIALGLPSILLPLQILWI
                     NLVTDVAPALALALDPAEPDVMSRPPKKRQEDILTRWHMANIVFYGLVISLGVFAAYA
                     FSVRLHPGDPARATEICFHTLVFAQLFFVFNVRNRSLLGDPGQIFANPWLLAGVGFSA
                     LLQFNISHVPIMQEVLDIVPLSVTEWGIVIASALLPTLVAQARKVLRNGMVG"
     gene            3114..4172
                     /gene="mnmA"
                     /locus_tag="PK146_02270"
     CDS             3114..4172
                     /gene="mnmA"
                     /locus_tag="PK146_02270"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPE91096.1"
                     /translation="MKAVVGISGGMDSTAAAAIMLDRGFEVIGVHLRFFCSGEAEKRI
                     ERIASELGISILSLDVESLFNCKVRRPFFDDLRKGFTPNPCVICNEQVKFVSLVSVAR
                     DLGAELVATGHYARISSGLDEGAALLRGVDPEKDQSYMLYRIPVTILEKTCFPLGGIT
                     KREVRAFCEERFPGLFEGVGESKDICFIPGRKLEETVELNAGPFIPGRIVTTSGEELG
                     YHRGLYRYTIGQRKGLSLSGGPWFVVEKRMSLNELVVGRQGDLVVEEVFCSDPVWHVE
                     PVAGERLNGCQRYRCRPYPCILEQVDDRSFSCRFTEGVSGVAPGQSLVLYYNDRVYGG
                     GIIERAEKGGPPGHEDHR"
     gene            4156..6732
                     /locus_tag="PK146_02275"
     CDS             4156..6732
                     /locus_tag="PK146_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE91097.1"
                     /translation="MKTIDNLLPAEHLRKRTDPGSLGFETTLDVPCLEDLIGQERAVQ
                     AMTFGMGIKNRGYNIFVVGDHGSGRTTYSLERLQDQADSEPAPRDYIYAFNFSNPEEP
                     LAIDLPTGQAKELASHLEELVEELKSTLSKAFENSQYEDAKAQLVKDFQEQVNRIMEE
                     LRSWAKEKGFVIKRTPQGFVNIPMILKGQEKSDEGGKVKESEESVAQEQETEPDPSSS
                     EAPSNGREEMKEMMQEDFESLSEDQKKALQEISEEISQRTLEVLRKIRDLEKSLKERI
                     KELESEISRAAIAPYLQETREKFGREGKIGEWVDALSEDIISNFNMFIAAARDEGADV
                     DFSRYLVNIFVTNDPEGGAPVVWETNPTYYNLCGKVEYESRQGMLTTDFRKIISGVIH
                     RANGGYLVLQAEEILRNFMSWDALKRVLRTGELVIENLGEQLGVIPVSSLRPEAIPVD
                     LKVVMIGTRYLYHLLLIYDPEFQKLFKIKADFDTDMPRDPETEKQMACFVAGYAKKED
                     LLPFSSDAVAELIEWSARLSGDSERMSTQFNRIAELVVESSAWASQAKLDVAGAGQVR
                     RALKEKIFRTNLVEEKIRRAFEQDSIRILTEGEVVGQINGLTVVDMRDHMFGHPVRIT
                     ANVYMGAEGIVNIEREVKMTGPIHNKGLMILGSYLGRMFARDFPLSLTARITFEQTYG
                     GIEGDSASSTELYCILSALADLPLKQGVAVTGSVDQHGNIQPIGGVNEKIEGFFEYCR
                     LRGLTGDQGVIIPKQNVRNLMLVHDVSDAVKAGKFNIWAISSIDEGLLILTGTEPGKM
                     AEKGGYTPGSIYSRVYTKLKGWYEKASKQKNNSKAGKRKPEEDHDDQSEDDDEHGGGT
                     GE"
     gene            6729..7421
                     /gene="nth"
                     /locus_tag="PK146_02280"
     CDS             6729..7421
                     /gene="nth"
                     /locus_tag="PK146_02280"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPE91098.1"
                     /translation="MRVFARPTGTIVRSCLDDLEMTWRQGERVHPPCSDEPLDGLVLT
                     ILSQNTNDRNRDRAFASLKARFPSWGEVAAAPLQDIADTIRSGGIANIKAQRILQVLE
                     RVRSSFGTYSLQAMHEWETGRIREFLAGTPGVGAKTIACVLLFDLGRPAFPVDTHVAR
                     VSRRLGWADASMVPEVIQDLMEEIVPPRRYLEAHLNMITHGRNLCTARKALCNECPLS
                     DRCPFPEGRSAI"
     gene            7421..>8467
                     /locus_tag="PK146_02285"
     CDS             7421..>8467
                     /locus_tag="PK146_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPE91099.1"
                     /translation="MGPPFVVSCVPCSSYDREVVETAVREALRLCGSFPPLVAPVIVK
                     ANLLSPSDPSRAVTTHPEILRALSMELILAGCPDVMIGDNPGYIFRHQEAELFRKTGV
                     EEICREIPARFGLLSRGGFAEVSPPNPGKLETLRFSAELLKASCVIGVSKLKTHVETE
                     ITGAIKNMFGAADTDTRKRAHGARSRNFLPEAIVDIFSVRVPDMFVLDAVEGMEGYGP
                     SHGRARRVGWVAASLNGLALDFVQAVIMGFSDPLSIPLLSVASGRIDGPQKRDDIELR
                     GARWEELPCRGFKRAPSAVRMIPSFLRGAAHQLVSLKPVLESGACIRCGICMKVCPVE
                     AIEPLYGGWPRIDRK"
BASE COUNT         1964 a         2170 c         2533 g         1800 t
ORIGIN      
        1 tgtcggtgat gatggtgaaa cgcgggccgc agtttgtgca gttgatgaag ggataacgga
       61 atctcctgtt cccagggtcc cgcaactccg caagacagtc gctgcaggtt gcaatgtccg
      121 gaggtatgag ggcatattcc cctcccgaac cgtgactgtc gaggatctca aagggccttt
      181 tccggatgtc gtcgatcctt tcgtcgagaa cgatcgtgat tgactgaatg tggcccaggg
      241 tgggacaatc cgtctgcagg cgttctatgt aggaatcgat cccttcgggg tctccataca
      301 ggtcaattac gacaccggct gttgtgttcc ttacggaacc cccaagaccg atctcgcctg
      361 aaagcctgct gcagaagggg cggaacccga tcccctggac cactccggtt acttgaagtc
      421 tgacatgtct tatcaattct tacctccggt ctccgggaat tgtaatgccc agccgacaga
      481 ggctcaagcc cctgttccgg tgaaggtgcc ggaaaggcta tccgcaacga acaggaaagg
      541 agcattgaac cctatggacc tgaccaaagc ccatgcactg gatgccaagg agatccttgc
      601 tgctcttgat gtcgacccgg aaaaaggtct tgacgacctg agagtggaag agcttataga
      661 gcggtacggt cctaaccaga tcaaccctga agaacctgaa ccatggtgga agagcctgct
      721 tcggcagttc atgaccccca tgatctatct attgacagga gcttccctga taagccttgt
      781 catgaaggag accctcgatg cttttgctat ccttgtcgtc attctgatca acggaggtat
      841 cggtttcgta accgagttca gggcggaaaa ggctcttatg gctctaaagg cgatgatctc
      901 ccccacagca gtcgctcttc ggggaggtgt gatcaggacc gtaagtgccg caagtcttgt
      961 tccgggtgac atcattcttc ttgaggcggg ggatgtagtg ccagcagatg cccgtgtttt
     1021 tgaacagtat aacctggggg tcgatgaagc cgccctgacg ggagaatctc tccccgtaga
     1081 caagacagga gagatcttgc ctgtgggaac ggaacttgcc gacaggaaga actgtctctt
     1141 ctcgggaacc gcagtggtga gaggttcggg gagggcggtg gtcttcggga ccggaatggg
     1201 gaccgagata ggcaggatca gttccatgct ctctacggta agcaagtcgg ccactcctct
     1261 ggaagaacgc ctggcaagac ttagtggcct cctgataaga atggtcatag gcatagcgat
     1321 cctggtgacc ggcctcgggg tcctccaggg cagggggatc gtacgtatga tcgagacggg
     1381 aatagccctg gcggtcgcgg ccgtgccgga ggggctccct ttcgtagcca caatgaccct
     1441 cgccatcggt gtgcacagga tggcgaagag aaacgccctc gtcaggaacc tggcatcggt
     1501 ggagactctg ggttcaacta ctgtgatctg caccgacaag accgggaccc tcacgatgaa
     1561 cgacatggcc gtgaggtcca tggtccctgc ccacccggag tgtgaggatc tgctcctgag
     1621 ggtttccgtc ctttgcaaca aggcccacct cgaagaggat tcccaggttg gagatcccat
     1681 ggaggtggct ctgctcagat ccgctctctc tagaggtatt gacataggcg ctacgaggag
     1741 agcgacaccc attatcgggg aggagccctt tgattcccag accatgagaa tgatcacatg
     1801 gcacagggat ggggtcgcgg ccaagggagc cccggaaagg atactccaga tgtgcaggaa
     1861 ggtctggtca aaaaacgggc ttgtcacctt cagtgaagag tacaggagag agtggatgga
     1921 aaggatcgag gctatggcgg cacagggaat gaggaccctg gctttcgcct ggggggatga
     1981 tgagggggat ctttccttcc tgggagttgc cgggatcttc gatccgccca gacctgaggt
     2041 tagagattcg gtaaggtcct gtatcgatgc aggcatccat gtaatcatgg taacggggga
     2101 ccatctcgtc actgccaggt ccatcgctgc tgaagtcgga atacttggtc aaggggggag
     2161 caaggccctt tccggttctg aactggctga gatggaggag ggagcgatcg cttcacaggc
     2221 aagagagatc gccgtcgttg caagggtggc cccggagcac aagctcagga tagttagatc
     2281 gctccaggcc tccggtgagg tagtcgcaat gacgggtgac ggcgtcaacg acgcggtggc
     2341 tctcaaacag gccgatgtcg ggatagccat gggtatccag gggaccgagg tcagcaagga
     2401 ggcctccgat ataatcctgc aggatgaccg tttctcaact atagtgacgg ccatcggaga
     2461 gggaaggaag atcttcgaca acataaggaa atcagtgctc ttccttctgt gttgcaatct
     2521 gagcgaggtg atcaccgtcc tgggtggtat tgcactgggc ctcccgtcta tccttctccc
     2581 tctccagata ctatggatca accttgttac agacgtggcc ccggctctcg cccttgccct
     2641 ggatcctgca gaaccggacg taatgagcag acctcccaag aaaagacagg aggatatcct
     2701 cacaaggtgg catatggcta acatcgtctt ttacggtctt gtaatatctc tgggggtctt
     2761 tgcggcctac gctttctccg ttcgccttca tcccggtgat cctgcccggg ctacggaaat
     2821 atgcttccat accctggtct tcgcgcagct cttctttgtc ttcaatgtaa ggaaccgatc
     2881 actgctgggc gatccggggc agatttttgc aaatccctgg cttctggcgg gtgtcggttt
     2941 ttccgctctc cttcagttca atatcagtca cgttcccata atgcaggaag tgctggatat
     3001 cgttcctctt tcggtcacag agtggggtat cgttattgcc agcgcacttc tccctaccct
     3061 ggtcgcacag gcgagaaaag ttctccggaa cggaatggtg ggatgaactg gcaatgaagg
     3121 ctgtggtggg gatcagcgga ggaatggaca gtacagcagc tgcggcgata atgctggaca
     3181 ggggtttcga agttatcggg gtccatcttc gctttttctg ttcaggagaa gcagaaaaga
     3241 gaatagagag gatcgcttcg gaactgggga taagtatcct ctcgcttgat gtggagagcc
     3301 tcttcaactg taaggtccgg cgcccttttt tcgatgatct cagaaagggg ttcaccccca
     3361 acccctgcgt gatctgcaac gagcaggtga agttcgtctc acttgtgtcc gtagcccggg
     3421 accttggagc ggaattggtc gcaacagggc actatgcaag gatatcgtca ggcctcgacg
     3481 aaggcgctgc cctgttaagg ggtgtcgatc ccgagaagga ccagagctac atgctttaca
     3541 ggatacctgt gaccattctg gaaaagactt gtttccctct tggcgggatc accaagaggg
     3601 aagtacgggc gttttgcgaa gaacgcttcc cgggactgtt cgaaggcgta ggcgagagca
     3661 aggatatatg tttcattcca ggaagaaagc tagaagagac cgtcgaactt aacgcaggtc
     3721 cattcatccc gggcaggatc gtgacaactt ccggagagga actgggatac catagaggct
     3781 tgtacaggta caccattgga cagagaaagg ggctatccct ctccggcggt ccctggttcg
     3841 tggtggaaaa gaggatgtct ctcaatgaac ttgtggtagg aagacagggg gatctcgtgg
     3901 ttgaagaggt tttttgcagc gaccccgtat ggcacgtgga acccgttgcg ggagaaaggc
     3961 tcaatggatg tcaacgttac cggtgcagac catatccctg tattctggag caggtagacg
     4021 accgatcctt ttcctgtcgt tttactgaag gagtgagcgg ggtagcccca gggcagtccc
     4081 tcgtgcttta ttataatgac agggtatacg gtggtggtat cattgagaga gcggagaaag
     4141 gggggccacc aggccatgaa gaccatcgat aatctcttgc cggcggagca tcttagaaag
     4201 aggaccgatc cgggatcact gggttttgag acgacccttg atgtcccctg cctggaagac
     4261 ctgatcggcc aggagagagc agtgcaggca atgaccttcg gaatggggat caagaacaga
     4321 ggttataaca ttttcgtcgt cggagaccat ggtagcggcc gaacaaccta ttccctggag
     4381 aggttacagg accaggccga ttccgagccg gcccccaggg actacatcta tgccttcaac
     4441 ttcagtaacc ccgaagagcc acttgccata gatcttccta ctggccaggc caaagaactg
     4501 gcctcacacc tggaagaact ggttgaagag ctcaagagca ctctcagcaa ggccttcgag
     4561 aacagccagt atgaagatgc aaaggctcag ctggtgaagg atttccagga gcaggtgaac
     4621 cgcatcatgg aggagctgag atcctgggcg aaagagaagg ggttcgttat caagaggact
     4681 ccccagggtt tcgtgaatat ccccatgatc ctgaagggac aggagaaatc cgatgaaggt
     4741 ggcaaggtca aagagtctga ggagagcgta gcccaggagc aggagactga gcccgatccc
     4801 tcttcttccg aagctccgtc gaacggcagg gaagagatga aggagatgat gcaggaggac
     4861 ttcgagtctc tttcggagga ccagaaaaag gccttgcagg agatctccga ggaaatatcc
     4921 caaaggaccc ttgaagttct caggaagatc agggatcttg agaaatcctt gaaggagagg
     4981 atcaaggaac tcgaatcgga gatttctcga gccgccattg ccccctacct tcaggagacc
     5041 agagaaaagt tcggcagaga aggcaagata ggggagtggg ttgacgccct ttccgaggac
     5101 attatctcca atttcaacat gttcatagcc gctgcaaggg atgaaggcgc cgatgtggat
     5161 ttttccaggt acctggtcaa cattttcgtg accaacgatc ccgaaggagg ggctccagta
     5221 gtctgggaga ccaaccccac ctactacaat ctttgcggca aggtcgagta cgagagccgg
     5281 cagggcatgc tgaccaccga cttccgaaag atcatctccg gggtcatcca cagggccaac
     5341 ggtggctatc ttgtcctgca ggccgaagag atcctgcgga acttcatgtc ctgggatgcc
     5401 ctcaagagag tcctcagaac aggagaactg gtgatagaga acctggggga acagctggga
     5461 gtgatacccg tctcctccct gagaccggaa gccatacctg tcgatctcaa ggttgtcatg
     5521 atagggacga gatacctcta tcatcttctc ctgatatacg accccgagtt ccagaagctt
     5581 ttcaagatca aggcagattt cgacaccgat atgccgaggg atcctgagac cgaaaaacag
     5641 atggcctgtt tcgtcgcggg ctacgccaag aaggaagatc tccttccctt ttcttccgat
     5701 gcggtggcgg agttgattga gtggtccgcc cggctttcgg gggacagcga aaggatgtcc
     5761 acccagttca acaggatcgc agagcttgtc gtcgagtctt ccgcatgggc ttcccaggca
     5821 aagctggatg tagcaggagc cggacaggtc cggagggccc tgaaggagaa gatcttcagg
     5881 acgaacctcg tggaggagaa gatcaggagg gcctttgagc aggattcgat caggatactg
     5941 accgagggag aagtggtagg acagatcaac ggcctcaccg ttgtggacat gagagaccac
     6001 atgttcggac atcctgtgag gatcactgcc aacgtctaca tgggtgccga gggtatagtc
     6061 aacatcgaaa gggaggtcaa gatgacaggc ccgatccaca acaagggcct tatgatcctt
     6121 ggcagctacc tgggaaggat gtttgccagg gatttccccc tctcccttac ggccaggatc
     6181 acctttgagc aaacctacgg gggaatagag ggggatagtg cctcttcgac ggaactatac
     6241 tgcatccttt cagcccttgc ggatcttccc cttaagcagg gtgtggccgt caccggttcg
     6301 gtcgaccagc atgggaacat acagcccatt ggaggcgtca acgagaagat agaaggtttc
     6361 tttgaatact gtcgtttgag gggacttacg ggggaccagg gggtcattat cccgaaacag
     6421 aacgtaagga acctgatgct cgttcacgat gtatccgatg ctgtaaaggc ggggaagttc
     6481 aacatatggg ccatttcttc cattgatgag ggtcttctga tccttacggg gacagagccc
     6541 ggaaagatgg cagagaaggg tggatacacc ccgggaagca tctattccag ggtctacacc
     6601 aaacttaagg gttggtacga gaaagcctcg aaacagaaga acaactcgaa ggccgggaag
     6661 agaaagcccg aagaggatca cgatgatcag agcgaagatg acgatgaaca cggcggcggg
     6721 accggggaat gagggttttc gccaggccta cagggaccat tgtcaggtcc tgccttgacg
     6781 acctggagat gacctggaga cagggggaga gagttcaccc cccctgctcg gacgaacccc
     6841 tcgacggact tgtcctcacc atactctcac agaacaccaa tgaccggaac cgggacaggg
     6901 ccttcgcttc cctgaaggcc cgattcccct cgtgggggga ggtcgccgct gctccgttgc
     6961 aggatattgc cgacacgatc aggtccggag gaatagccaa catcaaggcc cagaggatcc
     7021 ttcaggtact ggaaagggtc cgttccagct tcggtaccta ttccctgcag gccatgcatg
     7081 aatgggagac gggaaggatc cgtgaattcc ttgcgggtac tcccggggtg ggcgcaaaga
     7141 ccatcgcctg cgtcctgctc ttcgatctgg gccggccggc cttcccggtt gacactcatg
     7201 tggcaagggt atcaagaagg ctgggttggg ccgatgcctc catggtcccc gaagtgatac
     7261 aggatctcat ggaggagatt gttcccccaa gacgttacct tgaggcccac ctgaacatga
     7321 tcacccacgg aagaaatcta tgtaccgcga ggaaggccct ctgcaacgaa tgccccctga
     7381 gtgaccgttg tcccttcccg gaaggaaggt cggcgatctg atgggtcctc ccttcgttgt
     7441 ctcctgcgtc ccatgctcct cttatgacag ggaggtcgtg gagactgcgg taagagaagc
     7501 ccttcggctc tgcggttcat tccctcccct tgtcgctccc gtcattgtca aggccaacct
     7561 tctctctccc agcgacccct ccagggctgt aacgacccac cctgagatcc tgagagccct
     7621 ctcaatggag ctgatcctgg cgggatgtcc cgacgtaatg atcggagaca accccggtta
     7681 tattttccgt caccaggagg cagaactgtt ccggaagact ggagtggagg agatctgccg
     7741 tgagatcccc gccaggttcg gacttctgtc caggggcggt tttgctgagg tcagcccccc
     7801 aaaccccgga aagctggaaa cgctgcgatt ttcggctgaa cttctcaagg cttcctgtgt
     7861 gatcggtgtt tcgaagctca agacccatgt ggagacagag atcacaggag ccatcaagaa
     7921 catgttcgga gctgccgata ccgataccag gaagagggcc cacggggcaa ggtctcggaa
     7981 cttccttccc gaggctatcg tggacatctt ttcggtcagg gtccccgaca tgtttgtcct
     8041 ggatgcagtg gaaggaatgg agggttatgg accctcccac ggaagggcaa gaagggtagg
     8101 ctgggtcgca gcctccctca acggtcttgc ccttgatttt gtccaggccg ttatcatggg
     8161 tttcagcgat cccctgtcga tccctcttct gtcagtagct tcgggaagga tcgatggtcc
     8221 ccagaagagg gatgatatcg aactgagggg tgcccgttgg gaggaactgc cctgcagggg
     8281 tttcaagaga gcgccttcag cggtgagaat gataccatcc tttctccggg gagcggccca
     8341 tcagttggta agcctgaaac cggtactgga aagtggtgcc tgtatccggt gcgggatctg
     8401 catgaaggtc tgcccagttg aagccataga gcccctctac ggaggatggc cccgtatcga
     8461 ccggaag
//
LOCUS       DAOPKQ010000037         8268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_9182_length_8268_cov_4.192378, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000037 DAOPKQ010000000
VERSION     DAOPKQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9182_length_8268_cov_4.192378"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1239)
                     /locus_tag="PK146_02290"
     CDS             complement(61..1239)
                     /locus_tag="PK146_02290"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HPE91100.1"
                     /translation="MLRRFAQEICKTTHDIIGHHVLVTDGNAVIIGSSDPSRLFTVHT
                     PSVECMRSRAETYTDREEAGRMEGVKPGVTLPVCLAGEVVGSIAIAGDHREVARYGYL
                     VQKQAELFIREQVIQESAGARDRAIQELTAHIASFAPGNDDPAILATRIRELGFEPDT
                     PRICLILEPGSDSPAMLDKLNDPVGGVSVRSGIREMIRYVFPEPASFCSSLGQTRFAV
                     FASTGASPGDPLEGSPGEGCRRCRENLEQMGLSLTVGAGSLARTVEDLHYSYGDAWKA
                     LTIGIKKNEPGKIHRIGDYLMEDLLTTVNVKKGRRFARNALSGLRKTPDGGEMLHTFR
                     RWCLHPCSPGIVAKELNIHRNTLHYRIEKIRKATGYDIRSFRDAFELFMAVTLEMLHE
                     "
     gene            1423..2709
                     /locus_tag="PK146_02295"
     CDS             1423..2709
                     /locus_tag="PK146_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HPE91101.1"
                     /translation="MRLELHKVSVRNVLWGDETKVERGTLFVNREEMLSVAMKDNRFA
                     RAELEFARPGESVRIIPVKDVIEPRCKLSGPGEVFPGFVGGMQTVGEGTTLVLDGAAV
                     VTCGPIVAFQEGLIDMSGPAADYSPFSQTNNVVLVVEPVEGLDQHSYEAALREAGFRL
                     GWYLADKCRDSKADEVKVYEMGGPVEESAKYPHLPKIAYFCMSITQGLLHDTFLYGAD
                     MKHLVPTLIHPNEKMDGAMVSGNCVSSCDKNTTWHHLNDPIVEELYSRHGKELVFLGV
                     IPTLEDTRLAGKERTSWFNANLARMLGADGVVVSEEGYGNPDTDLCMNAAKCEKLGIR
                     TVIISDEAAGTDGKSQGLADAAPEITAFVSAGNVNEMVELPVMDRILGYDSSISNLSG
                     GAEESLRKDGSMFVELQAIIGSTNELGFSRIGARWI"
     gene            2727..4037
                     /locus_tag="PK146_02300"
     CDS             2727..4037
                     /locus_tag="PK146_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3771..3773,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HPE91102.1"
                     /translation="MTRRVVCYINQFFAGMGGEEQAHVPPSARPGTVGPAMQVQSLLG
                     EEAVVVGTVICGDSYFGEHLEEARAECVRLIGEFKPDLLVAGPAFNAGRYGVACGDIC
                     SAVTEKLGIPSITGMYRENPGVELYSPKTFIVKTSDSARGMKDALGAISALAVKILKG
                     TPLGAAEDEGYFPRGIRKNFFHETNGAKRAVDMLLRKLAGKPFSTEYEMPVFKRIPPA
                     EPVKDLKNATIALVSSGGIVPRGNPDHIRVSSAESYGVYDISSLDDLTPENFESIHGG
                     YDRVWANEDPDTVLPLDIMREMEREGVFGKLYDNVFTTTGTGTAVAHAERFGQEIGER
                     LKKDGVDAVILTSTUGTCTRCGASMVREIENSAGIPVVQIATIVPIMLTVGANRIVPG
                     VAIPHPVGDPSRGAEGDRAVRRELLERALRAIQTPVEEQTIFTS"
     gene            4170..4529
                     /locus_tag="PK146_02305"
     CDS             4170..4529
                     /locus_tag="PK146_02305"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HPE91103.1"
                     /translation="MKKILVVTNNPLAFEKYENSRKVEGSPVEVVEEASRMMLEGYSL
                     LGSPLPPNGRLMKNPYRSIALVEEKGQLKNGRDLLLLESARQRLRETPFLSSIGGRGK
                     DLAFMDLELLETSLGHR"
     gene            4608..6113
                     /locus_tag="PK146_02310"
     CDS             4608..6113
                     /locus_tag="PK146_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPE91104.1"
                     /translation="MPEFERLERIITGRLEENASKPAVWWKGSWMNGAEFLGLIANTE
                     KILEESGFGPGSRIAIFMANSPLLWAIAVAAWRLGGAIAPLNARSGINASLKIVEHID
                     PVGAVLGDGMDSLAKTLEGAGIPAVIAPPVGPLPGFKGRKDTREDESTAVIFATSGTT
                     GAPKAVQVSHGNLVDNSLKVHENIEGFDTGRILMNVLPNFHSLGFTICGVLPLCYGLP
                     MVIVSSFIPVKETLDALMAAEVSILIAVPTMLPFLLGAVLKEGISFPHLKYILTGGGK
                     LDPTLEGRIRKDMGVICFEGYGLTECSPVVSCNPSEKKRKLGTVGPPLPGYEIQIRGL
                     EGEILPGSEEGVLWVKGPSVTSGYFRAPELTAERFHDGWLDTGDIVKIDEDGFISILD
                     RATDLIIVGGFNVYPQEVETVLNAHPAVKMSAAIGISSSMSGEVVKAFVVPFEGTEVT
                     QHDLVQYAKEHLAHYKVPRKIDIVDELPLSSVGKVLRRKLREDEKAAGGEN"
     gene            6132..>8268
                     /locus_tag="PK146_02315"
     CDS             6132..>8268
                     /locus_tag="PK146_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HPE91105.1"
                     /translation="MGRTWLEDLALKSFPAEKRMIPGTGPDVMLRLKVPSALKRTIGT
                     AGLLAVLIISALSLSAASATSAVSFRAGLQPEGARLVIDLDRPVKYSLSAEGSKVIID
                     IRAESAEERSGNFPNNRVASSFSARPTAGGTSLEINLRSPVVTVKHYTLPSPERIVVD
                     ISPSPGATGQDGQNPRVFGIHGLVRAPLVTGPGKGYTDLRLSPEEMVLEEGFTARTFP
                     FEVPFTRRIVEGSSFIPVLVFDADEGEAQARVSFILNGVPVGDTLLQVRKGSATGTRI
                     ELPPGLIRPGVNRMTLAVSAYPESGRGPTGGTLRILDGSFLRLRYLSASDVRLKDIGA
                     FIDGSLSPEGEDLAVVLPERPEPEEIQAALEMVMEWNGGRPSGQIRARILTPGLVDSE
                     TARYFHTVYLGRSPLLPLPVLQAFRSETPPGDKGFLSAFVDSYGKFRLLVTSDTAGGV
                     LSGTLSLMDLSLRDRFDTRRAFITPGKTLSARETEEADGTFTLKELTGGDIVLSAAGT
                     RERRMILTPPPVGKIGGPGEVSLTFRSSHLLDGERSTLGIELNGAPADTVKLGRSSAS
                     EKDRLTAKIPEKLLGQGPLEIGLRAVLEPAGGKERELPGDVFWVVVDGSSQVRTPLST
                     RRLPSLLEDLPYQLTGGEISLFVGPEPMDGMLSAVAGILADWQRTLGKALEVKVKPLS
                     QLEWENLKGDAIVASDLEKILSLGIPIAAV"
BASE COUNT         1787 a         2276 c         2524 g         1681 t
ORIGIN      
        1 actggagccc ctgtctttgc gggagtcaat atcggctctc tgcgcaatgg tccatgaggg
       61 tcattcgtga agcatctcca gggtcaccgc catgaagagc tcgaaggcat ccctgaaact
      121 cctgatgtcg tagccggtag ccttcctgat cttttcgatc ctgtagtgca gcgtgttcct
      181 gtgaatatta agttccttcg caacgatacc ggggctgcag gggtgcaggc accatctccg
      241 aaaggtatga agcatctccc ctccgtcagg ggtcttcctg agccccgaaa gggcgttgcg
      301 tgcgaaacgc cttccctttt tgacattgac cgtcgtgagc agatcttcca tcaggtaatc
      361 gcctatccgg tggatcttcc cgggttcgtt cttcttgatc cctatggtca gggccttcca
      421 ggcatcgccg tatgaatagt ggaggtcttc taccgtcctg gccagagatc ccgctccgac
      481 tgtcaaggac agccccatct gctcaaggtt ctccctgcac cttctgcaac cctctccagg
      541 ggagccttcc aggggatccc ctggagaggc cccggtggaa gcaaagacag cgaaccttgt
      601 ctgtcccagg ctcgagcaga agcttgcggg ttcggggaag acgtacctga tcatctcccg
      661 tataccgctc cggaccgaca ctccacctac agggtcgtta agtttatcca gcattgcggg
      721 gctgtcggac ccgggctcca gtatcaggca tatcctgggg gtgtcgggct cgaagcccag
      781 ttcccttatc ctggtggcca gaatagcggg gtcgtcgtta cccggtgcga aggaggcgat
      841 atgtgctgtc agttcctgga tcgccctgtc cctggcgcct gccgactcct ggatgacctg
      901 ctccctgatg aagagttcgg cctgtttctg tacaaggtag ccgtaacggg ctacttccct
      961 gtgatcccct gcgatggcta tggaacccac aacctctcct gcaaggcaga ccggcagtgt
     1021 gacgccgggt ttgacgccct ccatgcgtcc ggcctcttcc cggtctgtat aggtctcggc
     1081 ccgggacctc atgcattcga ctgaaggagt atgtaccgtg aaaagccgcg aaggatcgct
     1141 gcttcctatg atcacggcat tcccgtcagt gaccagtacg tggtggccga taatgtcgtg
     1201 agtggttttg cagatctcct gtgcgaagcg cctgagcatt ggcaacgcct ccctttcctt
     1261 gcaaccgatt atcgtccata accccttttt gtgcaaaagc acaaaaaagt gccttttaaa
     1321 agggttcaag tccttgaagg tgaacagtat caacccttgg tcagcaggcc tataatctgg
     1381 gggaaagaag caatgacagc aagcctggag gggggatggc ccatgaggtt ggaattgcac
     1441 aaggtttcgg tcaggaatgt cttatggggc gacgagacga aggtcgagag ggggaccctg
     1501 ttcgtcaacc gtgaggagat gctctccgta gccatgaagg ataacaggtt cgccagggcg
     1561 gagctggagt ttgcgcgccc tggcgagagc gtaaggatca taccggttaa ggacgttatc
     1621 gagccccgct gcaaactttc cggcccgggg gaagttttcc cgggtttcgt gggagggatg
     1681 cagaccgtag gtgagggaac cacactggtc cttgacggtg cggccgtcgt tacctgcggt
     1741 cccattgtgg ccttccagga agggctcata gacatgtccg gccccgctgc ggactactct
     1801 ccattttccc agaccaataa cgtggtcctg gtagtggagc ccgtcgaagg cctcgaccag
     1861 cactcctacg aggcggctct ccgcgaggca ggtttcaggc tggggtggta cctggccgac
     1921 aagtgcaggg actcaaaggc agatgaagta aaagtctatg aaatgggagg gcccgtagaa
     1981 gagtccgcca aataccctca ccttccaaag attgcctatt tctgcatgtc cataacccag
     2041 gggcttctcc acgatacttt cctctacggt gcagatatga agcacctggt accgaccctt
     2101 attcacccca acgagaagat ggatggtgcc atggtatcgg ggaactgcgt atcatcctgc
     2161 gacaagaaca ctacgtggca ccacctgaac gacccgatag tggaggagct atacagcagg
     2221 catgggaagg aactggtctt cctgggcgtc atcccgaccc tggaagatac aaggcttgcg
     2281 ggcaaggagc ggacctcgtg gttcaacgcc aacctcgcca ggatgctcgg ggctgatggg
     2341 gtggtggtat ccgaagaggg ttacgggaac cctgatactg acctctgcat gaacgccgcc
     2401 aagtgcgaga agctggggat caggacggtg ataatttccg atgaggctgc cggtaccgac
     2461 ggaaagagcc aggggttggc cgacgccgcc ccggagataa cggccttcgt ctccgccggg
     2521 aacgttaacg agatggtaga acttccggtc atggacagga tactgggtta cgacagctcc
     2581 atatccaacc tctcaggcgg tgccgaggag agtctccgga aggacggttc catgttcgtt
     2641 gagctgcagg cgatcatcgg ttccactaac gagctcggct tcagcaggat cggggcccgg
     2701 tggatatagg agagggggcg gtgaaaatga ccaggagagt agtttgctat atcaaccagt
     2761 tcttcgccgg catgggggga gaggaacagg cccatgtgcc gccttcggcc cgccccggga
     2821 ccgtgggccc ggccatgcag gtacagagcc tcctcggaga agaagccgta gtggtcggga
     2881 cggtcatctg cggggacagc tatttcggcg agcatcttga agaggctagg gctgagtgcg
     2941 tcaggttgat aggggagttc aagcctgatc ttctcgttgc ggggcccgcg ttcaacgccg
     3001 gccgctacgg agtggcctgc ggagatatct gttcggccgt gaccgaaaag ctcggcatcc
     3061 cttccatcac gggcatgtac agggagaacc cgggcgttga gctgtacagc cccaagacct
     3121 tcatcgtgaa gacctctgac agcgcccgag gaatgaagga tgctctcggt gcgatatcgg
     3181 cccttgccgt gaagatcctg aaggggaccc ctctcggtgc cgcagaggat gaagggtatt
     3241 tcccgagagg gatcaggaag aacttcttcc acgagaccaa cggggcgaag agggctgttg
     3301 acatgctgct caggaaactt gcaggaaagc ccttttccac cgagtacgag atgcccgtat
     3361 tcaaacgcat accgccggcg gaaccggtga aggacctgaa aaatgccacc atagcactgg
     3421 taagctccgg aggcatagtc ccgcggggga accccgacca tataagggtc tcatcggcag
     3481 aatcctacgg cgtttacgac atctccagcc ttgacgacct gaccccggag aacttcgagt
     3541 ccatccacgg tggctatgac cgggtgtggg ccaacgagga tcccgatacg gtcctccctc
     3601 tcgacataat gagggagatg gagagagagg gagtgttcgg caagctctac gataacgttt
     3661 tcaccaccac agggacaggt accgccgtgg cccatgccga gaggttcggc caggagatcg
     3721 gcgaaaggct caagaaggac ggcgtagatg cggtcatcct tacctccacc tgaggtacct
     3781 gtacccggtg cggtgcatcg atggtaaggg agatcgagaa ctcggcaggg ataccggtgg
     3841 tccagatagc caccatagtt cccataatgc tgacggtggg agcgaacagg atcgtgcccg
     3901 gtgttgccat tccccaccct gtgggcgacc cctcgagagg cgccgagggc gacagggcag
     3961 tgaggcggga acttctcgag agagccttga gggcgatcca gacacctgta gaggagcaga
     4021 ccatcttcac ctcctagaaa ggctgggggg atctgttcgt tcgatgcggg gcgggaggct
     4081 tgggccttcc gccccttgtt tctggaaaag gaaggcccgt ggaggttata attgatcatc
     4141 cgcagactcc ggggagtgga tctgtcccta tgaagaaaat tcttgtcgtt accaataacc
     4201 ctcttgcttt tgagaaatac gaaaattcgc gtaaagttga ggggtcaccc gtcgaagttg
     4261 tcgaagaggc ctcgaggatg atgctggagg gttattccct gctgggatcc cctctgcctc
     4321 cgaatggaag gctgatgaaa aacccttacc gttcgatagc tctcgtggag gagaaaggcc
     4381 agttaaagaa tggcagggac cttctcctcc ttgaaagtgc caggcagcgc ctaagggaaa
     4441 cccccttcct gtccagtatc ggggggcggg gcaaagatct ggcctttatg gacctggagc
     4501 ttctcgagac tagcctgggt caccggtgaa ggggagaacc cttcgtggat aatgataaaa
     4561 gggatggtat cctccgaggg ccatccagag ggggagaggg aaaaacaatg ccagaattcg
     4621 agaggctcga aaggatcata accggacgtc ttgaagagaa tgcttccaaa cctgcggtat
     4681 ggtggaaggg gtcctggatg aacggggccg aattcctcgg gctcatcgcg aacacagaga
     4741 agatcctgga agagagcggg ttcggtcctg gatcaaggat cgcgatcttc atggccaatt
     4801 cacccctcct ctgggcaatt gccgtggccg catggaggct tggaggggct atagcccccc
     4861 tcaacgcaag gagcggcatc aacgccagtc tgaagatagt ggaacacatc gatcccgtcg
     4921 gggcagtgct cggcgacgga atggacagcc tggccaagac ccttgaaggg gccggtatcc
     4981 cggccgttat cgctccccct gtaggcccac tgccgggctt caaagggcgg aaggatacca
     5041 gggaagatga aagcacagcc gttatcttcg ctacgtccgg tactactgga gcccccaagg
     5101 ctgtgcaggt gagccacggg aaccttgttg acaactccct caaggtccac gagaacatcg
     5161 agggctttga cactggcagg atcctgatga acgtcctccc caacttccac tcactgggtt
     5221 tcaccatctg cggggtattg cccctttgtt acggtctccc catggtcatc gtctcatcct
     5281 tcattccggt gaaggagacc cttgatgccc tcatggcggc tgaagtgagt atcctgatag
     5341 cagtaccgac catgctcccg ttcctgctgg gtgctgtcct gaaggagggt atttccttcc
     5401 cgcacctgaa atacatcctc accgggggag gaaaactcga cccgacgctt gagggcagga
     5461 taaggaagga tatgggggtc atctgcttcg aaggctacgg gttgacggaa tgctctccag
     5521 tagtctcatg caacccctcc gagaaaaaaa ggaaactggg aactgtaggg ccgcctctcc
     5581 cggggtacga gatacagata aggggccttg aaggcgaaat ccttcccggg agtgaagaag
     5641 gagttctgtg ggtgaagggt ccctcggtga cctcgggata cttccgggcg cctgaactga
     5701 cggccgagcg gttccatgac ggatggctgg acacgggaga tatagtgaag atagacgagg
     5761 atggcttcat atccatcctg gacagggcca cggacctgat catcgtcgga gggttcaacg
     5821 tctaccccca ggaggtggag acggtgctga acgcgcaccc ggcggtcaag atgagcgccg
     5881 ccatagggat ctcttcctcc atgagcgggg aagtggtcaa ggccttcgtt gtgccctttg
     5941 aaggaacaga ggtgacccag catgacctgg tacagtacgc aaaggagcac ctcgcccact
     6001 acaaggtacc caggaagata gacatagtgg acgagctgcc cctttcgagc gtaggaaaag
     6061 tactcaggag gaagctgaga gaggacgaga aagccgccgg aggggagaat tagtgaagca
     6121 gggaagaacc gatgggaagg acatggctcg aggatttggc cttgaagtcc tttcctgcgg
     6181 agaagagaat gatcccgggt accggccctg acgttatgct gagactgaag gtcccctcgg
     6241 ccttgaagag aaccatcggg acggcaggac ttcttgccgt cctgatcatc tccgccctct
     6301 ctctctcagc ggcgagtgct acttctgccg tctcattcag ggccggtctt cagcctgaag
     6361 gggcaaggct ggtgatcgat cttgacagac ccgtcaaata ctcgttgtca gcggagggtt
     6421 caaaggtcat tatcgacatc agggccgagt ccgctgaaga gcgatcgggg aacttcccga
     6481 acaacagggt cgcatcgtcc ttttccgcca gacccactgc cgggggtact tcccttgaga
     6541 taaatctgag gtctccggtg gtcacggtga agcactacac tctccccagc cccgaaagga
     6601 tcgtcgtgga catctcccct tcccccggag caaccgggca ggatggccaa aacccccggg
     6661 tattcggcat ccacggcctt gttcgggcac ctcttgtcac aggcccggga aaggggtaca
     6721 ccgatctcag actctccccg gaggagatgg tcctggaaga gggtttcacc gcaaggacct
     6781 tccccttcga agtccccttt acgagaagga tagttgaggg cagttctttc atccccgttc
     6841 ttgttttcga tgcagacgaa ggagaggctc aggcaagggt gtccttcatc cttaatggcg
     6901 tccccgtcgg cgataccctc cttcaggtca ggaaaggatc tgccaccgga accaggatag
     6961 aactgccccc cggtctgatc cggccggggg tgaacaggat gaccctggct gtgagcgctt
     7021 accctgagag cgggagaggc ccgaccgggg gaaccctcag gatccttgac gggagtttcc
     7081 ttcgcctgcg gtacctctct gcttctgacg tccgcctgaa ggacataggc gcattcattg
     7141 atggaagcct ttcccctgag ggcgaagacc tggctgtggt attgcccgaa agaccggagc
     7201 cggaagagat ccaggcggcc cttgagatgg tcatggagtg gaatggaggc agaccctccg
     7261 gccagatccg ggccaggata ctcacgccgg gactggttga tagcgaaact gccaggtact
     7321 tccacacggt ctacctgggc aggagtcccc ttttgccgct gccggtcctt caggctttcc
     7381 gttcagagac cccgcccgga gacaagggct tcctttcggc cttcgtggat tcctatggca
     7441 agttcaggct cctggtgact tccgataccg ccggaggtgt actttcggga accctgtccc
     7501 tgatggatct atcacttcgg gaccggttcg atacgaggag agccttcatt actccgggga
     7561 agacactttc cgccagagag acggaagaag cggacggaac ctttacctta aaggagctga
     7621 ccggcggcga cattgttctc agcgctgccg ggacacggga acggagaatg atcctcacgc
     7681 ctcctcctgt cggaaagatc ggagggccag gggaagtttc tttgacattc cgttcttcgc
     7741 accttctaga cggtgaacgg tccacccttg gcatcgaact taacggcgca cccgccgata
     7801 ctgttaaact gggcaggtcg tccgcctcgg agaaggatcg gctgacggcg aagatcccgg
     7861 aaaaacttct cggccagggc cctctggaga tagggttgag agccgttctt gaaccggctg
     7921 gagggaagga aagagaattg ccaggggatg tcttctgggt cgtagttgat ggctcgtcac
     7981 aggtgcggac ccccctgtcg acaaggcgac tgccctcctt gctggaggac cttccctacc
     8041 agctgacagg cggtgagatc tctcttttcg tgggtcccga accgatggat ggaatgcttt
     8101 cagccgttgc tggcatcctc gctgactggc agaggactct ggggaaagcc ctggaggtca
     8161 aggtcaagcc actgtcacaa ctggagtggg agaacctgaa aggcgacgcg atagtcgcgt
     8221 cagacctgga gaagatcctc tccctgggga tccccattgc agccgttc
//
LOCUS       DAOPKQ010000038         8002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_9582_length_8002_cov_3.957217, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000038 DAOPKQ010000000
VERSION     DAOPKQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_8002_cov_3.957217"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="PK146_02320"
     CDS             <1..1980
                     /locus_tag="PK146_02320"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPE91106.1"
                     /translation="VHPRDEKNRALVGRKVRVPVAGRVVPVIEDMMVDPGFGTGLVKI
                     TPAHDPNDFLVGQRHGLEAIQVIDEHGAMNREAGPLFEGMDRIEARKKMLFLLEEEGL
                     LEKAEDHEHAIGHCYRCSAVIEPYLSEQWFVKAAPLAAPGIEAVREGRIRIIPDQWVG
                     TYNNWMENIRDWCISRQLWWGHRIPAWTCEDCGHVTVSEEDPDRCESCGSTRILQDED
                     VLDTWFSSALWPFSTLGWPDDTQDLRTFYPTSLLVTGFDILFFWVARMIMMGLKFMQE
                     EPFSDVYIHALVRDEQGQKMSKSKGNVIDPLEIIDEFGADSLRMTLASLTIQGRDIFL
                     SPERIESSRLFMNKLWNASKLALGNLEGQEVANLPGSASLRQHDRWIIRRVNEVNEQV
                     LDHLKGYFYGEAARTLYQFVWNELCDWYLEMAKPALRGEEGGKRQTDTRIVLARVFDS
                     TIRMLHPFIPFLTEELWETFGFGKGSLIEEQGWPELLSPETRTAMEVFEPMDVFQELV
                     RSIRNLRAEARLHPQQEVPEVIVVLSDSAVNEVGEVIDSNSDLVGLLCRVGEIRFMQK
                     GSPRPSGSLMSVTGFGEVFLVVGHILDIKAEMARLSTELEKARRDLARSEKKLADPSF
                     LSRAPGEIVEKERERLTQTEEKIKVIERNMESLAG"
     gene            1991..3319
                     /locus_tag="PK146_02325"
     CDS             1991..3319
                     /locus_tag="PK146_02325"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPE91107.1"
                     /translation="MNRTIFGTSDPVEILLHEMSTPYIRPGLSRIAALLGKMGHPERS
                     FPVVHVVGTNGKGSVSAMIEAIFLDAGYSTCLYTSPHLSDITERLRFSGTPIDRELLI
                     ASLKRIGSLQGEGMDPGSRSTYFEVLTAAAFDAIATYSPDVAVIEAGMGGRLDATNMA
                     RNVVLTVITTIGSDHAHFLGGTLEEIAMEKFRVLRPDGISVFSGRPFCLEGAYLDLCR
                     RIGNDGETLRSVKISDPTIALEGNTFDISIRGTDPFSVRTSLGGTFQVENAALALLAT
                     KRLSSLYPRIDKKAMIGGLGKTRWSGRLERIDHHGREIVLDGAHNPEGIQALVETFNI
                     MGTAQDHAVVFAVMRDKDLGGMVSRICNAFPMVVFTSIPGMERAACPDFLQEISRSFP
                     GNCESLSVNDPIDALQVAFRRYRKVVVCGSLYLSGYLKRFLFPDRQGGMG"
     gene            3316..4137
                     /locus_tag="PK146_02330"
     CDS             3316..4137
                     /locus_tag="PK146_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPE91108.1"
                     /translation="MKSISPWGWRKTSVEGQQVINYLMAPPLDAIMAVTLFLRGPVMD
                     QADGCPLRASRLLFGEERPPLLIAPLQEHGTRMIEALPSVSLPARPEGDALLLRRNDV
                     LGSLRFGDCIPVVAVAEEQDPWVLLVHSGFLGTARGVVREALDKAMRSTGLRCLDSVH
                     FWVGPGIGSCCYSRRLDDPRTREGLEFFPRQCIRIEGDLVFFDLPGAILITLYDINVP
                     PSNVVLIDRCTCCSPESFYSYRRGDRTGRSLLLAGFREGFHKSDLWWENRFEGVE"
     gene            4170..5141
                     /locus_tag="PK146_02335"
     CDS             4170..5141
                     /locus_tag="PK146_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPE91109.1"
                     /translation="MDPVRVGVIGVGHLGFHHARVYTELLNTDVVGIVDNNPERAAAV
                     GELLRVPFYSDIEKFYRHAMPDAVSVVVPTVHHYETAKRALSSGVNVLIEKPVTSTVE
                     EAEELLQLAATQNLVLQVGHIERFNSAVQHVRNIIKDPLFLQSRRIGPFSSRISDVGV
                     VLDLMIHDIDIILSLVHSEILSISAMGRSVRSNLEDIATAQIAFQNGALAQILVSRVS
                     ERRLRQLEIMEPERFVTINYETQDVSIHRCVQEKECGLVEVIEHPVFPKREPLKLELQ
                     HFVTCVREGKQPLVGIMDGKRALEVAIAILKQIQTPGRAEEIPAEMA"
     gene            5277..5489
                     /locus_tag="PK146_02340"
     CDS             5277..5489
                     /locus_tag="PK146_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HPE91110.1"
                     /translation="MSSVNVQALVALFLFVLCLVLSRMIVNISSGKWPGGALWVTYLR
                     VLLGFLFAAAITLGMYSFAGIDFLNR"
     gene            5538..6476
                     /gene="meaB"
                     /locus_tag="PK146_02345"
     CDS             5538..6476
                     /gene="meaB"
                     /locus_tag="PK146_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPE91111.1"
                     /translation="MDRIIERALKGDPRSIARLITAVESENDRAKEIMQKIYPHTGKA
                     HMIGVTGSPGAGKSTFLDKLISSLMVSGLTVGVIAVDPSSPFTGGAVLGDRLRMQQHS
                     LDPGVFIRSMGTRGSLGGLSRGTYEAALILDACGKDIVLIETVGVGQSEIDIVKIADT
                     VALLLVPGMGDDIQIMKAGIMEIADVFIVNKADRDGSERVVREVNIMLDLQTEKDWRP
                     PVIPTVAESGEGVALAAGALSEHREFLASSDEGRRRKYRRLRLEVEEILKREIAKIAE
                     EAWDSRIGGDVLEDLASRRKDPYTVADELLSMFISR"
     gene            6567..7667
                     /gene="upp"
                     /locus_tag="PK146_02350"
     CDS             6567..7667
                     /gene="upp"
                     /locus_tag="PK146_02350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPE91112.1"
                     /translation="MRIALGSDHAGFQLRNCIREFLQDSSYFTVDFGAEGSDRPCDYP
                     DAAFEVAVRVARSEYDLGILICGTGIGMSIAANKIPGIRAALAYDEETGRLAREHNNA
                     NILALGGRKTDCETAIRIVKAWLSADFEASRHLLRIDKIKSFEEEAAMIRDRLNSPPR
                     LVTFDHPLIQHKIGIIRDRNTSVKAFRELVQEIAGLMVYETTRNLQLEPVTVETPITT
                     TTAYSLSGKKIAIVPILRAGLGMVDGILQLIPNAKVGHIGLYRDPETLEPVEYYCKLP
                     GDIGERDIIIVDPMLATGGSAVAAIDLVKKKGGKKVSLLSLIAAPEGVARVHERHPDV
                     DIFTAALDSHLNDHGYIVPGLGDAGDRLFGTR"
     gene            7702..>8002
                     /locus_tag="PK146_02355"
     CDS             7702..>8002
                     /locus_tag="PK146_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HPE91113.1"
                     /translation="MERTVFFLVFLVWSLTVTPLSIYLSRQYRIIDEPGGRKKHSMVT
                     PRGAGVVIWAGYLLYAVLFPDPVNGVRIIATGATAIFLFGYMDDMHPLPPVLRLFF"
BASE COUNT         1850 a         2098 c         2328 g         1726 t
ORIGIN      
        1 gtccaccccc gggatgaaaa gaaccgtgcg ctggtgggca ggaaggtacg ggtacctgtc
       61 gccggaaggg tagtacccgt gatcgaggac atgatggttg accccggttt cgggacgggt
      121 ctcgtcaaga tcacccctgc ccatgatccc aacgactttc tcgtaggaca gagacacgga
      181 ctggaagcga tccaggtcat agacgagcac ggtgccatga acagggaagc aggtcccctc
      241 ttcgaaggca tggaccggat cgaggccagg aaaaagatgc tcttccttct ggaggaggag
      301 gggcttctcg agaaagccga ggatcacgaa catgctatag gtcactgcta ccggtgtagc
      361 gccgtgatcg agccctacct gtcagagcag tggttcgtca aggcggcccc tcttgccgcc
      421 cccgggatag aagctgttcg agaaggaagg atcaggataa tccccgatca atgggtcgga
      481 acctacaaca actggatgga gaacatcagg gactggtgca tatcccgaca gctctggtgg
      541 ggtcacagga tacccgcctg gacttgtgag gactgcggtc acgtcaccgt ttccgaagaa
      601 gatccggaca ggtgcgagtc ctgcggcagc accaggatct tacaggacga ggatgtcctc
      661 gatacctggt tcagcagtgc cctctggccc ttctcgaccc ttggctggcc cgatgacacc
      721 caggacctga ggacctttta cccaacaagc ctccttgtga ccggtttcga tatcctcttc
      781 ttctgggtgg caaggatgat aatgatgggg ctcaagttta tgcaagagga gcctttcagc
      841 gacgtctata tccatgccct ggtcagggat gaacaggggc agaaaatgag caaatcaaag
      901 ggcaatgtca tcgatcctct ggagataatc gatgaattcg gggccgattc tctcaggatg
      961 accctggcaa gcctcaccat ccaggggagg gatattttcc ttagccctga aaggatagaa
     1021 agcagcaggc tcttcatgaa caagttatgg aatgccagca agctggccct tgggaacctc
     1081 gaggggcagg aagtggcgaa ccttccgggg tcggcttcgc taagacaaca tgacaggtgg
     1141 atcataagga gggttaacga ggtcaacgaa caggtcctgg atcacctgaa gggatatttt
     1201 tacggggaag cagccaggac cctctaccag ttcgtatgga acgaactttg cgactggtac
     1261 cttgaaatgg ccaaaccggc cctgaggggg gaagagggag gcaaaaggca gacagacacc
     1321 aggatcgttc tggcgcgggt ctttgacagc actataagga tgcttcaccc cttcatcccg
     1381 ttcctgactg aggaactctg ggaaaccttc gggtttggaa aggggtcact gatagaggaa
     1441 cagggctggc ccgaacttct ttcaccggaa acccgtaccg caatggaggt tttcgaacct
     1501 atggatgtat tccaggagct ggtgaggagc atccggaacc tgagggctga agccagactt
     1561 catccgcagc aggaagtccc cgaggttatc gtggtccttt ccgacagtgc agtcaacgaa
     1621 gtgggagagg tgatagactc caactccgat cttgttggcc ttctctgccg tgtaggggag
     1681 atcaggttca tgcagaaggg ttcaccgagg ccgtcggggt ccctgatgtc cgtcacgggc
     1741 ttcggggagg tatttcttgt ggttggccat atcctcgata tcaaggcaga aatggcccgg
     1801 ctctcgacgg aactggagaa agctcgaaga gaccttgcca ggtcggagaa gaagctcgcc
     1861 gatccctctt tcctatcgcg ggctcccggt gagatcgtag aaaaggagag ggagagactc
     1921 acccagacag aggaaaagat caaggttatt gaaagaaaca tggagagcct tgcaggatag
     1981 ctgatctgcc atgaacagga cgatcttcgg gacatcggac cctgtcgaga tccttctgca
     2041 tgagatgtcg accccctaca tcaggccggg actgtcgagg atcgccgcat tactgggcaa
     2101 aatgggccat cccgagaggt cattccccgt tgtccacgtt gttgggacca acgggaaggg
     2161 ttccgtttcc gctatgatag aggccatttt ccttgatgca ggctattcga cctgtctcta
     2221 cacaagcccg cacctttcgg atataacgga acggctgagg ttctcgggta ccccgatcga
     2281 ccgggagctc cttatcgcct ctctaaaaag gatcggttct ctgcagggag aaggaatgga
     2341 ccctgggtcc aggtccacct acttcgaagt cctgacggcg gccgcctttg acgcgatcgc
     2401 tacttactcc ccggacgttg cagtcatcga ggcgggaatg ggggggaggc tggacgccac
     2461 gaacatggcc aggaacgttg tccttacggt catcaccacc atagggtcag accacgccca
     2521 tttccttggc ggaacccttg aagagatagc catggaaaaa ttcagggtcc ttcgtcctga
     2581 cggtatcagt gtcttctccg gaaggccttt ctgtcttgaa ggggcatatc tggatctttg
     2641 ccgaaggata ggcaacgacg gagagaccct gcgttcagtg aagatcagcg accccacgat
     2701 tgccctggag ggtaacactt tcgatatatc catcaggggt acggaccctt tttccgtcag
     2761 gacatccctc ggggggacct tccaggtgga gaatgcagcc ctggctcttc tggcgaccaa
     2821 gaggctttcc tccctgtacc ctcgtatcga taaaaaagca atgatcggtg gtctgggaaa
     2881 gacccgctgg agcggcaggc ttgaaaggat cgatcaccat ggtcgtgaga tagtccttga
     2941 cggggcccat aaccctgagg ggatacaggc cctggtggag accttcaata tcatgggaac
     3001 cgcccaagat catgccgttg tctttgcggt catgagagac aaggacctgg gcggaatggt
     3061 ctcccggatc tgcaatgcct tccccatggt ggtctttact tccataccgg gtatggagag
     3121 agcagcttgc cctgatttcc tgcaggagat atcaaggtcc tttcccggaa attgtgagag
     3181 cctttccgtt aacgatccca tagacgcact ccaggtggcc ttccgacgct acaggaaggt
     3241 cgtcgtctgc gggagtctct atctttcggg atacctgaaa aggttcctgt tccccgaccg
     3301 ccaagggggc atgggatgaa gtctatatca ccctgggggt ggaggaagac atccgttgaa
     3361 ggtcagcagg tcatcaatta cttaatggct ccaccgctag atgccattat ggcagtaacc
     3421 ctctttctca ggggacctgt aatggaccag gctgacggct gccctttacg ggcctcccgg
     3481 ctacttttcg gtgaagaaag accccccttg cttatagccc cgctccagga acacggaacc
     3541 agaatgatag aagccctccc ctctgtatcg ttgcctgcca gacctgaagg agacgccctt
     3601 cttctgagaa ggaacgatgt actcggaagc ctccggttcg gtgactgtat tcccgtcgtg
     3661 gccgttgcgg aggagcagga cccctgggtc ctccttgtac actccggttt ccttggaact
     3721 gccagggggg ttgttcggga agccctggat aaagccatga ggtccaccgg cctgagatgc
     3781 ctggatagtg tccatttctg ggtaggtccc ggaattggta gctgttgcta ttcaagaagg
     3841 ctggacgatc cccggacccg ggaaggtctg gagtttttcc caaggcagtg catcaggata
     3901 gaaggggacc tggtcttctt tgatctcccc ggtgccattc ttataacttt atatgacata
     3961 aatgttcccc cttccaacgt ggtccttatc gaccgctgca cctgttgttc accagagagc
     4021 ttctactcct atcgaagagg tgacaggaca ggaaggtcac ttcttcttgc gggtttccgc
     4081 gaaggattcc acaaatccga tctttggtgg gagaataggt ttgaaggggt agagtagttt
     4141 taagagcata cccagtggaa ggtgatcgaa tggacccggt aagagtagga gtgatcggtg
     4201 tagggcatct tgggttccac catgccaggg tctatacgga actcctgaat accgatgttg
     4261 tgggaatagt cgacaacaat ccggagaggg cagctgccgt gggcgaactg ctgagggtcc
     4321 ccttttattc cgatatcgag aagttctaca ggcatgccat gcccgatgct gtcagcgtgg
     4381 tggtcccgac ggtccatcac tacgagacag caaagagggc tctcagttca ggcgtgaatg
     4441 tccttatcga gaaacctgtc acttccaccg ttgaagaagc tgaagagctt ctgcagctgg
     4501 ccgccaccca gaaccttgtt ctccaggtag gacatattga acgcttcaat agcgcggtcc
     4561 agcatgtaag gaacataatc aaggatcctc tcttcctcca gtcaaggagg attgggccct
     4621 tcagctcccg gataagtgac gtgggcgtag tgcttgacct catgatacac gacattgata
     4681 tcattctctc cctggtacat tcggaaatat tgtccatttc ggccatggga aggtcggtaa
     4741 ggtccaatct cgaggatatc gccaccgccc agatagcctt ccagaacggt gcccttgcgc
     4801 agatacttgt cagccgagtc tccgaaagaa ggctgaggca gctggagatt atggaaccgg
     4861 agagattcgt caccatcaac tacgaaacgc aggacgtatc gatccatcgc tgtgttcagg
     4921 agaaggagtg tgggctcgtg gaggttatcg agcaccccgt cttcccgaag agggagcctc
     4981 ttaaactgga gcttcagcac tttgtcacct gcgtcaggga gggcaaacag ccactggttg
     5041 gcattatgga cgggaagagg gccctggagg tggcaatagc cattctcaag cagatccaga
     5101 cccctggcag ggcggaagag atcccggctg agatggccta ggttcgggaa ctaacttgat
     5161 actagagccg ccgaaaggcg gcttttttga tgcacggacc acgggaggcg ctaggcatct
     5221 tttgctgtta caatacaaag ccggagagtg gtccatacga tagatggggg taaaaaatgt
     5281 cctcggttaa tgttcaggcg ctggtggctc tgtttctttt cgtcctttgc ctcgtccttt
     5341 ccaggatgat agtgaacata tcctccggga agtggcccgg cggagccctt tgggtcacct
     5401 atctgagggt ccttctgggc ttcctcttcg ccgccgccat caccctgggg atgtactcct
     5461 tcgccgggat agatttcctg aacaggtaga acttcccttg cgggtccata tcacctttcg
     5521 aaaggattgt tgaaaaattg gacaggatca ttgaaagagc cctgaaggga gatccccgct
     5581 cgatcgccag acttatcacc gcggtcgaaa gcgagaacga tcgcgctaaa gagatcatgc
     5641 aaaagatcta ccctcacaca gggaaggccc acatgatcgg tgtcacgggt agccccggcg
     5701 cagggaaaag cacctttctt gacaaactta tctcctcctt gatggtttca ggtctgaccg
     5761 taggggtcat tgcggtcgat ccatcaagcc ccttcacagg tggggcggtc ctcggcgaca
     5821 ggctgaggat gcagcagcac agtctcgatc cgggagtttt catccgcagc atgggtacaa
     5881 gggggtccct gggggggctc agtcgtggaa cctacgaagc ggccctcatc cttgacgcct
     5941 gcggcaagga catagtcctt atcgagacag tgggtgtagg acaatcggag atagacatcg
     6001 tcaagatcgc cgacaccgta gcactgctcc tcgtccctgg catgggcgac gacattcaga
     6061 tcatgaaggc gggcataatg gagatcgccg atgtcttcat tgtgaacaag gcagacaggg
     6121 acggttcgga gagagtggta cgggaggtca atatcatgct tgacctccag acggaaaaag
     6181 actggagacc cccggtgatc cctactgtcg ctgagagcgg agaaggggtg gcccttgccg
     6241 ccggagcgct ctcggaacac cgggaattcc tggcctcttc cgacgagggc aggcgccgaa
     6301 agtacaggcg tcttcgcctg gaagtggagg agatactaaa aagggagata gccaagatag
     6361 ccgaagaagc atgggatagc cgcatcggtg gtgatgtcct tgaagatctt gcttcgcgaa
     6421 gaaaggatcc ctacaccgtg gccgacgaac tcctgtcgat gttcatctcc cgctgactgt
     6481 ccatcaagga cctacggtta tcaaggcggc agtcgggcaa gtgatatcat cataaacgaa
     6541 gcgatcaaaa gacaggaggc ttggggatgc gtatcgcact tggctcggat cacgcaggtt
     6601 tccaactcag gaattgcata agggaattcc ttcaggactc atcatacttt accgtggatt
     6661 ttggtgccga aggatcggac aggccctgcg actaccccga tgcggccttt gaggtagcgg
     6721 tcagggttgc cagaagcgag tacgacctcg ggatccttat ctgtggtacc ggtatcggta
     6781 tgtcgattgc agccaacaag atcccgggta tccgtgccgc tcttgcctac gacgaagaga
     6841 cgggaaggct ggcccgggaa cataacaatg cgaacatcct tgccctcggg gggagaaaga
     6901 cggactgtga aacagcgatc cggatagtga aagcctggct ctcggcagat ttcgaggcct
     6961 cccgtcacct ccttcgtatc gacaagatca agtcctttga agaagaagca gcgatgataa
     7021 gggatcgcct gaacagccct cccaggctcg taaccttcga tcacccactt atccagcaca
     7081 agatagggat catccgggac aggaacacgt cggtaaaagc cttccgtgaa ctggtccagg
     7141 agatagcagg cctcatggtc tacgagacca ccaggaacct gcagctggaa ccggttaccg
     7201 ttgagacccc gataacgacc actacggcct actccctatc cggaaagaag atcgcgatcg
     7261 ttccaatact gagggcggga ctgggcatgg tcgacggaat actgcagctc attcccaatg
     7321 ccaaggtagg tcacatcggg ctctaccggg accccgaaac tctggagccg gtggagtact
     7381 actgcaaact cccaggagat atcggcgaaa gggacatcat aatagtggac cctatgctgg
     7441 ctaccggagg ttctgccgtg gcggccatcg acctggtcaa aaaaaagggc gggaagaagg
     7501 tatcccttct cagcctgatc gctgcaccgg agggtgttgc cagggtccac gaaagacatc
     7561 ctgacgtgga catctttacg gcggccctgg acagccatct caacgatcat ggctacatcg
     7621 tacccgggtt gggtgatgcc ggcgaccggc ttttcgggac cagataacag accccaactg
     7681 ttaagggggt gatcagggtt ggtggaaaga acggtttttt tcctggtgtt cctggtctgg
     7741 tccctgaccg tgacgccttt atccatctac ctttcacggc agtacaggat catcgatgaa
     7801 ccgggcggaa gaaagaagca ctctatggtg accccaaggg gagccggggt tgtcatctgg
     7861 gctggctatc tgctttacgc tgtcctcttt cccgatcctg ttaatggtgt caggatcatt
     7921 gccactggag ctacagcgat cttcctgttc ggctacatgg atgacatgca ccccttgccg
     7981 ccggtattga ggctcttctt cc
//
LOCUS       DAOPKQ010000039         7878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_9794_length_7878_cov_4.389620, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000039 DAOPKQ010000000
VERSION     DAOPKQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9794_length_7878_cov_4.389620"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..155)
                     /locus_tag="PK146_02360"
     tRNA            complement(81..155)
                     /locus_tag="PK146_02360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Gln,seq:ctg)
     gene            317..1585
                     /locus_tag="PK146_02365"
     CDS             317..1585
                     /locus_tag="PK146_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPE91114.1"
                     /translation="MDQDRRSFRELLEKRPLNVQSVFGSEPVALVSGRDIAAAARSVG
                     SIVLASNVRNPLTIKGVLKAARELEAFVMLELAKSEATYCGCTFENVPQLAARYSSEL
                     GGGVLFGLHVDHYAIRSREDLLASIPHLRKLVELGWTSVAVDASHNPDWENLCFTRDV
                     AMHIPAYLGLEVEVGEIKGPGELTTVEEALFFIGGLNSWRIFPDFLAISNGSKHGTYD
                     STAGEVEGIDLERTREIAEAVSPFGVTIAQHGISGTPLDKVSRFSEFGINKGNVGTLW
                     QNIVFGLEMDPVTGNAVVKDGSYVKDPARGITLELWEEIVKWADGQGLSRKSGDYKKA
                     NRPFDEAIMALPEGIHGRIVEETAEWAKRFIRAFHAEGSSEALMEIVSERPDHNAAPE
                     QAVIASRKDHYREIAPDRGAAKDKGKDFSD"
     gene            1705..3417
                     /locus_tag="PK146_02370"
     CDS             1705..3417
                     /locus_tag="PK146_02370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPE91115.1"
                     /translation="MSTKDLRRRPGKKGPAGNELRFIPLGGLGQIGKNMFVLECGEDI
                     IVVDCGLMFPDEEMLGIDFVIPDASYLFENRERIRGLILTHGHEDHIGSLPFILPRID
                     VPIYGTKLTLAMVEAKFRDSCPDYTPVSVEVGSGERFSLGCFSVTFLSVCHSIPDGVG
                     LAMETPAGTVIHTGDFKLDPTPVDGRITDYSAFAEYGRKGVLLMLSDSTNVERPGFTQ
                     SERIIGRTLERLLRLHRNRRVVIAAFSTNLHRAQQILQVASRFNRKVALMGRSMVQNV
                     NLAIDLGYIDVDKETLVPMEEANRVPDNKLIVLTTGSQGEPFSGLVRMSKGEHRFINL
                     GPGDLVAVFASPIPGNERLVSRTINRLFECGCEVVYDRDQEVHVSGHAAREELKMMLS
                     IVRPQYFVPLHGEYRHLVRHAKLAQEMDIPQKNTFVLQNGDVLSLNSSKAQVRRGAVP
                     SGGILVDGMALGEMQNSLLKERQEISEEGIVIISLALDSEFRPVARPLIESVGFLHMD
                     DAEELRGDLVLEIERALEKLPGRTGRDEDKIAQKIRSRARNVLRRYSRTPPVIRVQVM
                     VVER"
     gene            3480..5099
                     /locus_tag="PK146_02375"
     CDS             3480..5099
                     /locus_tag="PK146_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPE91116.1"
                     /translation="MTTGLFFNLLGGVGLFLFGIKLMGESLQDLAGDRMRRLISALTS
                     SPVKGVIVGALITALIQSSSATTVMTASFVHAGLMTLKQSVGVIMGAAIGTTITAQLI
                     AFKIKEFALPLIGFGMLFAVFGRSKRQKFIGNCIVGFGLLFVGMQTMENAMSFLRDRQ
                     DIFLAFQHHPLLGVLAGTVLTMLVQSSSATVGLTIVMASQGLLTIDSAIPILFGDNIG
                     TTITAVLASVGLNRSARQSALAHVLFKVIGVLLFLAFLGPFRQLVLLTSTDIARQLAN
                     AHTIFNVLNTLLFLPFVEPFVRLVRAIIPDVGGAILMGPRFLDRNLISASPIAAVEAV
                     RKELVRMGLLALDMIRDVRTAFLENDRRMIEQVLQTERVVNELTREITAFSTDLMQVS
                     LPPVLSSSLSFYVNGVGDIERIGDHAENLIELYEYKIEHNLPFSDLAMEEFEIMIGLS
                     EKAVRLSVEALDTAEDSKVQQVLKLEDEIDAMERDLRKRHIDRLNQGVCNPGSGVIFI
                     DILSNLERVGDHANNIALIVKDINVVRGKEA"
     gene            5104..5910
                     /locus_tag="PK146_02380"
     CDS             5104..5910
                     /locus_tag="PK146_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPE91117.1"
                     /translation="MRLQCALLGLVQGITEFLPVSSSGHLVIVQELLGWSDPALSFDV
                     MLHLSTMTATLLFFRKEVVRIARDWFSGLFSIRDRDLPGWRYGWSVILGTLVTVFIAL
                     LSRPLAEVLFSSPLFAGFALIITGFVLRYGSRIVPRDLPVSLSTGIKVGLAQGLSILP
                     GISRSGMTIVTGLRCGLSPREAFSFSFLLSLPAILGATVLEVVKAGGISEVVTSLPTG
                     WWIGMATAFASGLFALGILRRVVIRGRLGFFAVYCIAAGLFVVFLNLIGG"
     gene            5915..6157
                     /locus_tag="PK146_02385"
     CDS             5915..6157
                     /locus_tag="PK146_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91118.1"
                     /translation="MARTADSRWWILLLCVILGGFIGVYLQRFPLTERFFRDIIDIGF
                     DLKSLDLIVVDFGLRLYVRMNLGTFLGGVTGVLMIR"
     gene            6154..6768
                     /locus_tag="PK146_02390"
     CDS             6154..6768
                     /locus_tag="PK146_02390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPE91119.1"
                     /translation="MIRKGFLSSCDVVLASASPRREVLLRELGWSFRVVPPVLEERIL
                     EGLAPSGQVRRLAEEKAGSVFSSHANSLVIAADTMVVLGSVILGKPGSRDDAFDMLSL
                     LSGKTHSVITGVAVRWRDRSVSASEETLVSFRELSEAQIRAYLSGGESGDKAGAYAIQ
                     GWGSLLVSSIRGCYFNVVGLPLFRLSRLLESVGIPLEEQWGERE"
     gene            6765..>7878
                     /locus_tag="PK146_02395"
     CDS             6765..>7878
                     /locus_tag="PK146_02395"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HPE91120.1"
                     /translation="MRIRQALFFAAALGAVAVLAAVGLIPRIRAEGEGSVAALVTDVR
                     DVVSLARESDLPVPEALDELLDRGLTAVAVGELTGQELVAGMLAMEYGSVEDLLPEGI
                     PQGLFPDSAAMMIRRESCCSDEIRSFVSLKFPGARTISLEKGDLYVLPLSLAETLEAG
                     MLPDYPMLNMLKGKDIPLVFRPGSTPGIPGENVALAVASVLDAYPGIRAVVPAGLFVA
                     GYPELGPLSEVLKERHIPVSKVEFSQQIGIGFLERGLYPDILSLHSVRREEILSRRLS
                     REDLIDRFVRASRERSVKILYVRPSDLHSGSRLERFGEECKRISGRLDTLGIRTGWPR
                     TLDPWSASFFAALALALVLVVLTLRLLSRFLSFEEGS"
BASE COUNT         1620 a         2112 c         2379 g         1767 t
ORIGIN      
        1 ccaacgggat tcgaacccgt gttacaggac tgagaaccct gggtcctaga ccactggacg
       61 atggggccat tccggttatt tggctggggg acagggactc gaaccccgac ttcttgatcc
      121 agagtcaagc gtcctgccaa ttagacgatc ccccaaggat cgctccctta gcgcgccgca
      181 tatagtatca cgggtcaagc cgggccgtca atgacaaaac gttgatcgat cgttcagaaa
      241 ggatggatcg tgaaccggtc caggtccctc gcttaagtgt acaatgaacg cggacatttc
      301 cgggaggtgt tttgaaatgg atcaagacag aagatctttc cgtgagttgc ttgaaaaacg
      361 gccccttaac gtccagtccg ttttcggcag cgaacccgtg gccctggtca gcggaaggga
      421 tattgccgcg gcggcgaggt cagtgggttc aatagtactg gcatcgaacg ttcggaaccc
      481 gctgacgatc aagggcgtcc tgaaggccgc ccgggaactt gaagccttcg taatgcttga
      541 actggccaag tcagaggcta catactgcgg gtgcaccttc gagaacgttc cccaattggc
      601 tgccagatat tccagtgaac tgggaggggg ggtgttgttc ggactacatg tggaccatta
      661 cgccataaga tccagggagg accttctggc ttccatccct cacctgagga agcttgtgga
      721 gcttggatgg acctctgtgg cggtcgatgc atcacacaat cctgactggg agaacctctg
      781 cttcaccagg gacgtggcca tgcatatccc agcctacctc gggcttgagg tagaggtcgg
      841 ggagatcaag ggacccgggg aactgaccac tgtcgaagag gccctgttct ttatcggagg
      901 gcttaactcc tggaggatct ttccggactt tctggccatc tccaacggga gcaaacacgg
      961 tacatacgac agtaccgcag gagaggtcga ggggatcgat ctcgaacgga ccagggagat
     1021 agcggaagcc gtctcgccct tcggcgtcac catagcccag cacggcatct cggggacacc
     1081 cctcgacaag gtgtcccgat tctccgaatt cgggatcaac aagggtaatg ttggcaccct
     1141 ttggcagaac atagtctttg ggctggagat ggaccctgtc accgggaacg ctgttgtgaa
     1201 ggacggatcc tacgtcaagg acccagcgag agggatcacc cttgagctct gggaagagat
     1261 tgttaaatgg gccgacggtc agggcctttc ccgcaagtcc ggagattaca agaaagccaa
     1321 cagacccttc gacgaagcaa tcatggccct gcctgagggg atccatggga gaatagtgga
     1381 agagaccgct gaatgggcga agaggttcat aagggccttc cacgccgagg gtagttcgga
     1441 ggcgctgatg gagatagtgt cagaacgccc cgatcataat gccgctccgg aacaggccgt
     1501 gatcgcttcc agaaaggatc attaccgtga gatcgccccc gacaggggag cggcgaagga
     1561 caagggaaag gatttcagcg attgatccgg agcgggccca aagtccctga tgctttcaag
     1621 ggctcccgcc atgcccgggg catttctttc ctgtcgtggt attggccgca ggtcgtggta
     1681 ggatagtcaa ccggaggtgg gccgatgtca acgaaagatc ttcgcagaag acctgggaag
     1741 aagggccctg cagggaatga actgcggttc atacctctgg ggggactggg acagatagga
     1801 aagaatatgt tcgtactcga atgtggcgag gacattatcg ttgttgactg cggacttatg
     1861 ttccccgacg aggagatgct gggcattgat tttgtcatcc ccgacgcctc gtacctcttc
     1921 gagaaccgtg agaggatccg gggtctcatt ctgacccatg gtcacgagga ccatatcggg
     1981 agtctcccct tcatcctccc ccggatagac gttcccatat acggtacgaa acttaccctt
     2041 gccatggttg aagccaagtt ccgggactcg tgtcccgact atacacctgt ttcggtcgag
     2101 gtcggatccg gcgagaggtt cagcctgggc tgcttttctg taaccttcct ctcggtctgc
     2161 cactcgatcc ctgacggtgt cggtctggct atggagacac cggcaggtac cgtgatccat
     2221 acaggggact tcaagctgga cccgaccccc gtggatggaa ggatcaccga ttattcagcc
     2281 ttcgcggaat acgggagaaa gggagtcctt ctcatgctct ccgattcgac caacgtggaa
     2341 cggccaggat tcacccagtc ggagaggata atcgggagga cccttgagag gcttttgagg
     2401 ctccacagga acaggagagt ggtgatagca gccttctcca ccaatcttca ccgggcccag
     2461 cagatactcc aggtggcatc aaggttcaac cgaaaagttg cacttatggg gcggagcatg
     2521 gtgcagaacg tgaaccttgc cattgacctg ggctatatcg atgtcgacaa ggagaccctt
     2581 gttccgatgg aggaggccaa ccgtgtaccg gacaacaagc tgatagtcct gaccaccgga
     2641 agccagggtg agcccttttc aggtcttgtg aggatgagca agggggaaca ccgcttcatt
     2701 aaccttggtc ccggtgacct cgttgcggtc ttcgcctcgc ccattcccgg gaacgaaagg
     2761 ctggtcagca gaaccatcaa ccgccttttc gagtgcggtt gcgaggtggt ctacgaccgc
     2821 gaccaggagg tgcatgtttc cggccacgca gcgagggagg agctcaagat gatgctcagc
     2881 atagtgaggc cacaatactt tgtccctctc cacggagagt accggcacct tgtcaggcat
     2941 gccaagctgg cccaggagat ggatatcccc cagaaaaaca cctttgtgct tcaaaacggc
     3001 gatgtcctca gtctcaattc cagcaaggcc caggtccgcc ggggtgcggt gccctcgggc
     3061 gggatccttg ttgacggaat ggccctgggc gagatgcaga acagccttct caaggagagg
     3121 caggagatct ccgaggaagg tatcgttata atatcccttg ccctggatag cgagtttcga
     3181 cctgtagccc ggccccttat cgagagcgtc gggtttctcc acatggatga tgcagaggag
     3241 ctgagaggcg atcttgttct tgagatagaa cgagccttgg aaaagcttcc tggtcgcaca
     3301 gggagagacg aggacaagat agcccagaag atcagaagca gggcccggaa cgttctgaga
     3361 aggtacagca ggacaccgcc ggtgataaga gtgcaggtca tggtcgttga gcggtagtcc
     3421 cttttctatc attgcagggt cgtatctatg ggatccggcc tgacggggga gatgaaggtt
     3481 tgactacggg actctttttc aacctcctgg ggggtgtagg tctcttcctc ttcggaatca
     3541 aactgatggg ggagtcgctg caggacctcg caggtgacag gatgaggcgc ctcatctcgg
     3601 cgctaaccag ttcacccgta aagggcgtta tcgtgggggc tctgataacg gcgctgatcc
     3661 agagcagcag tgcgacgact gtcatgaccg ccagttttgt ccatgcggga ctgatgaccc
     3721 tgaagcagtc tgtcggcgtg atcatgggag cggcaatagg gaccactatc acggcacaac
     3781 tgatagcctt caagatcaag gagttcgctc ttcccctcat cggcttcggg atgctcttcg
     3841 cagtcttcgg gaggagcaag cggcagaagt tcatagggaa ctgtatagta ggtttcgggc
     3901 ttctcttcgt aggtatgcag acaatggaga acgccatgtc cttcctgaga gaccgccagg
     3961 acattttcct tgccttccag catcaccccc tgctgggcgt gctggcaggg acggtcctta
     4021 caatgctggt ccagtccagc tcggccacgg taggtctcac catcgtcatg gcttcccagg
     4081 ggcttctgac cattgactcc gccataccca tcctcttcgg tgacaatatc ggtaccacta
     4141 tcacggcagt attggcctcg gtggggctga accgctcggc gaggcagtca gccctggccc
     4201 atgtcctgtt caaggtgata ggcgtgcttc tcttcctggc cttccttggc cccttcaggc
     4261 agctcgtgct gctgacctcc acagacattg cccgacagct ggccaatgcc cataccatct
     4321 tcaacgttct caacactttg ctgtttttgc ctttcgtgga accctttgtt cgcctcgtga
     4381 gggctattat ccccgatgtc gggggagcga tacttatggg tccccgtttt ctggacagga
     4441 acctgatatc ggcctcgcca atagccgccg tggaagctgt acgcaaggaa cttgtgcgga
     4501 tgggccttct tgctcttgac atgatcaggg atgtacggac cgccttcctc gagaacgacc
     4561 gaaggatgat cgagcaggtt ctccagaccg agagagtggt caacgaactt acaagggaga
     4621 tcacggcctt ttcgacggac ctgatgcagg tcagcctccc tccggtcctc tcctcgtctc
     4681 tctctttcta cgtcaacgga gtgggtgata tcgaaaggat cggggaccac gccgagaacc
     4741 tgatcgagct ctacgaatac aagatcgagc acaacctccc cttctccgat cttgccatgg
     4801 aggagttcga gatcatgata ggcctttcgg agaaggccgt aaggctctct gtagaagccc
     4861 tcgacacggc cgaagacagc aaggtccagc aggtcctcaa gctggaggat gaaattgacg
     4921 cgatggagag ggatctgaga aagagacata tcgacaggtt gaaccagggg gtctgcaatc
     4981 ccggttcagg agttattttc attgatattc tcagcaacct tgaaagggtt ggagaccacg
     5041 ccaacaacat cgcgctcata gtcaaggaca taaacgtggt ccgcggcaag gaggcatgat
     5101 ccgttgcgcc ttcagtgcgc cctgttgggc cttgtccagg gtattactga attcctcccc
     5161 gtaagcagct ccggccacct ggtgatagtg caggaacttc tcggttggtc ggaccctgcc
     5221 ctctcttttg acgtaatgct tcatctgtcc accatgaccg cgacgcttct cttctttcgg
     5281 aaagaagtgg tcaggatcgc gcgggattgg ttctcgggac tcttttcgat aagagaccgc
     5341 gatcttcccg gctggaggta cggatggagc gtaatactgg gtactctggt gaccgtcttt
     5401 attgcccttc tctccaggcc cctggcggag gtcctctttt catcgcccct gttcgcaggt
     5461 tttgccctca ttattacggg atttgtcctt cgctacggca gcaggatcgt tcctcgcgac
     5521 cttcccgtct cgttgtcgac ggggatcaag gtaggacttg cccagggcct gtctatactg
     5581 ccagggatat caagatcggg aatgacgatc gttacagggc tccgctgcgg tctttccccc
     5641 cgggaggcat tctccttttc cttcctgctg tcactcccgg cgatccttgg cgccaccgtc
     5701 ctggaggtgg tcaaggcggg ggggatttcg gaagtggtca catcactgcc gacagggtgg
     5761 tggattggca tggcaacagc atttgcttcg ggccttttcg ccctggggat ccttcgaaga
     5821 gtggtcatca ggggccgtct tggcttcttt gccgtctact gcatcgcggc gggtcttttc
     5881 gtagtgttcc tcaatctgat agggggatga taaaatggcg agaactgcgg attccagatg
     5941 gtggatactt ctcctctgcg tgatccttgg cggttttata ggtgtctacc tgcagaggtt
     6001 ccccctcacg gaaaggttct tccgtgacat tatcgacatc gggtttgacc tgaagagcct
     6061 cgatctcata gtagtggact tcgggctcag gttgtatgtg cgaatgaacc tgggaacctt
     6121 cctcggaggg gtgacaggag tactgatgat cagatgatcc gaaagggttt cctgtcttcc
     6181 tgtgatgttg ttctggcttc ggccagccct agaagggaag tcctcctcag ggaactgggg
     6241 tggagtttca gggtggtgcc tcctgttctg gaggagcgga tcctggaagg ccttgctccc
     6301 tccggtcagg tccggagact ggcggaggag aaggccggct cggtcttttc ctcacatgca
     6361 aattctcttg tgatcgcagc ggacaccatg gtcgttctcg gatcggtcat actcgggaaa
     6421 ccgggaagcc gggatgacgc tttcgatatg ctcagccttc tatcagggaa gacccactcg
     6481 gtgatcactg gcgtcgccgt cagatggagg gacaggtccg tatcggcgag cgaagagacc
     6541 ctggtctctt tcagggagct ttcagaagca cagatcaggg cctacctgtc agggggagag
     6601 agcggggata aggccggggc ctatgctatc caggggtggg gctcgctgct tgtgagttct
     6661 atcaggggct gctatttcaa tgttgtgggg cttcctcttt tcaggttgag caggcttctg
     6721 gagagtgtag ggatccctct cgaggaacaa tggggtgaga gagagtgagg ataaggcagg
     6781 cattgttctt tgcggcggca cttggtgccg ttgcggttct ggcggcagta gggctcatcc
     6841 cccggatcag ggctgaaggc gaggggagtg tggctgccct ggtgaccgac gtcagagatg
     6901 tggtcagcct cgccagggag tcggaccttc cagtgccgga ggctctcgat gaactgttgg
     6961 accgggggct aacggcagtt gcggtgggcg agctgacagg ccaggagctg gtggcaggaa
     7021 tgctggcaat ggagtacgga tcagtggagg accttctccc cgagggcatt ccccagggac
     7081 tattccccga ctcggcagcc atgatgatca gacgggagtc ctgctgctcc gatgagataa
     7141 ggtccttcgt ctccctgaag ttcccgggag ccaggaccat cagcctggaa aagggagacc
     7201 tttacgttct ccccctctct ctggccgaaa ccctagaggc cggcatgctc cccgactatc
     7261 ccatgctgaa catgctcaag ggaaaagaca tcccccttgt cttccggccc ggatctacgc
     7321 cggggatccc tggggagaac gttgctcttg ctgtggcatc tgtcctggac gcctaccccg
     7381 ggatcagggc ggtggtcccg gcggggcttt ttgttgcggg ttacccggag ctggggcccc
     7441 tctcggaggt actgaaggag cgccacatcc ctgtctccaa ggtggagttc tcacagcaga
     7501 taggcatcgg cttccttgag aggggccttt atcctgacat tctttccctg cacagcgtca
     7561 ggagagagga gatcctgagc agaaggctct ccagagaaga ccttatcgac aggtttgtca
     7621 gggcttccag ggaacgttct gtcaagatcc tttacgtccg gccctcggat cttcattccg
     7681 gttccaggtt ggagaggttc ggcgaggagt gtaaaaggat atcggggcgg ctggataccc
     7741 tgggcatccg tacggggtgg cccagaaccc tggatccctg gagcgccagc ttcttcgctg
     7801 ccctggcgtt ggcccttgtc cttgtggtcc tgaccctgag actgttatca aggttcctct
     7861 cctttgagga aggcagca
//
LOCUS       DAOPKQ010000040         7700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10132_length_7700_cov_4.344016, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000040 DAOPKQ010000000
VERSION     DAOPKQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10132_length_7700_cov_4.344016"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PK146_02400"
     CDS             <1..300
                     /locus_tag="PK146_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPE91121.1"
                     /translation="LTVMEIDVHGFKGADEVTYSWYLLDYFDVKTWNTSMSRCTGCSC
                     AIFARAVAKGYVKQKGVLPAEKLAADDDLYNYVIEEQQKRRIKYTASVKRVMNVR"
     gene            645..2153
                     /gene="nhaC"
                     /locus_tag="PK146_02405"
     CDS             645..2153
                     /gene="nhaC"
                     /locus_tag="PK146_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPE91122.1"
                     /translation="MSEKQQVKPSLGLSIGVFVAATVIISFGVLKLGVDAHIPIVFSA
                     VLVCIVGLTVLKMPWSQIEEGGLNAIAVALQAVVILMIIGMVIGIWIQSGVVPSLIYY
                     GLSILSPSIFLLATLLITSIVSISTGSSWTTAGTVGIALMGIAHGLGIPAPLTAGIVV
                     SGAYFGDKMSPLSDTTNLAPAVAGANLFDHIRAMVWSTGPTYLIVAAICVFLGMRYAG
                     GALDAEKISAMQQMMKAEFNIGLLGFLPPLLVIGLAVRKAPAIPGLFAGVLVAAIMSA
                     VQGNGIGDIIDVIHYGYGASVSGEFAGAEGEALLNMLSQFNLDIAPEMATEVGGMLSE
                     LVSRGGLDSMMWTISLIFCALFFGGVMEACGFLETIVGSITRQVKTVGGMMGSVIGTC
                     FLANLFLGDQYLALVIPGRMFKTAFEEKGLAPRMLSRATEDAGTLTSALIPWNTCGAF
                     QSSVLGVATLAYAPYAFLNYLNPIVSIIMSYMKIGIYWRKEDGTDIVAKEKP"
     gene            2327..3595
                     /gene="ablA"
                     /locus_tag="PK146_02410"
     CDS             2327..3595
                     /gene="ablA"
                     /locus_tag="PK146_02410"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPE91123.1"
                     /translation="MRMRDRKEIPLWKGVSEQEWNDWKWQVANRITTVEKLRQVIDIT
                     DDEAASIGRSLGKLRMAITPYYASLMDPDDPKCPVRIQAVPTEHETKLAKADLHDPLH
                     EDVDSPTPGLTHRYPDRGLLLITDQCSMYCRHCTRRRHAGETDRAYGEEQIKKCIEYV
                     RKTPTFRDVLLSGGDPLTVDDDRLEWVISELRKIPHVDFVRLGTRMPVVCPQRITDKL
                     CTMLRKYHPIWLNTQFNHPKEVTSETAEACAKLADAGIPLGNQTVLLKGINDCPYIIR
                     ELNQRLLKIRVNPYYIYQCDLSEGIEHFRTSVGKGIQIIEYLRGHTTGMAQPLFIVDA
                     PGGGGKIPVGPNYVVSQSDRKVILRNYEGVLTTYTEPDDNQSHPEELYHWEEYTARQR
                     QLSREGLIKLFEGELLSLEPAHLDRRERNK"
     gene            3635..4051
                     /locus_tag="PK146_02415"
     CDS             3635..4051
                     /locus_tag="PK146_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91124.1"
                     /translation="MEELLVSRSVTPFPKWMKWMVLLVGILLIGDGMRSFMFHKILVG
                     AVLAYISGYDKRIVLSPGGIVRQTRTWITTHSTTLPWDEVQYVNFAYRGSKMMCFFEK
                     DVTGLKVLFDRNDEPEVRRILELYIPDVETGVVGGS"
     gene            4243..5751
                     /gene="nhaC"
                     /locus_tag="PK146_02420"
     CDS             4243..5751
                     /gene="nhaC"
                     /locus_tag="PK146_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPE91125.1"
                     /translation="MVERTQVKPGLGLSLAVFAAAAVMISYGVLKLGVDAHVPIVFSA
                     VLVCIVGLTVLKMPWSQIEEGALNAIAVALQAVVILMIIGMVIGIWLQSGVVPSLIYY
                     GLSILSPSIFLLATLLITSIVSLSTGSSWTTAGTVGIALMGIAHGLGIPAPLTAGVVI
                     SGAYFGDKMSPLSDTTNLAPAVAGSNLFDHIRAMVWSTGPTYLIVAVICIVIGMKYAG
                     GTLDAEKISAMQQMMKAEFNIGLLGFLPPLLVIGLAVMKAPAIPGLFAGVLVAAVMSA
                     VQGNGLGDIINAIHYGYEATFASEVAGAEGEALLNMLSQADLSIAPEMATEVGGLLTD
                     LLTRGGLDSMMWSISLVFCALFFGGAMEACGFLETIVGSITRQVKTVGGMMGAVIGSC
                     FVANLFLGDQYLSLVIPGRMFKSSFEEKGLAPRMLSRALEDSGTLTSALIPWNTCGAF
                     QSSVLGVATLAYAPYAFLNYLNPIVSLVMSYLKIGIYWRQEDGTDVVAKKKP"
     gene            complement(5841..6149)
                     /locus_tag="PK146_02425"
     CDS             complement(5841..6149)
                     /locus_tag="PK146_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HPE91126.1"
                     /translation="MGKVVAEVVIVPHGTGSTSLSGYVAEVEKTLRQYGLETMLTPMG
                     TILEGDLDVVLRAVRAAHEIPFDQGALRVGTTLRIDERRDKDISMKSKVRSVEEKLGD
                     "
     gene            6257..7147
                     /locus_tag="PK146_02430"
     CDS             6257..7147
                     /locus_tag="PK146_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPE91127.1"
                     /translation="MEWEFAQRNLGKLVMPILVILGWYIADRIVVDLTRKLFEKGISR
                     VENGQTGHLEGHERASRIKRLETLKGLVTDLLRWGLGAVAFLTLLSTINVNIMPILTG
                     LGIAGLAISLAAQNIIRDFLNGIFVVVEDHYAVGDVVKIGEYFGVVEYFSLRTTHLSD
                     LDGNYIIIPNSRITELVNATKYWSQAQVVVGVSYDTDIRKALGVMEIVGRDIRQDFPD
                     KVRDDPQVQGILSFDASSISLRALIRTFPGEHWFIGREYRLRLKDAFDAEGITIPFPQ
                     MDVWIRSPEGFRITGQKTSA"
     gene            complement(7129..7443)
                     /locus_tag="PK146_02435"
     CDS             complement(7129..7443)
                     /locus_tag="PK146_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPE91128.1"
                     /translation="MLIKKGTRVFDPEGFSPRTLSVPHPRSFVGSDGEKVWFVVIDGR
                     DPWHSNGATIVETAAVAERLGLVDALNLDGGGSSSIWWMGKIVTSPPGGIVRPVPYAL
                     VF"
     gene            complement(7504..>7700)
                     /locus_tag="PK146_02440"
     CDS             complement(7504..>7700)
                     /locus_tag="PK146_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91129.1"
                     /translation="PDVWSYVTAAPVDGWEVTVKEGVVSGVRHSSTSNHFVTREGFLV
                     IARGVAGEQLRNTPPGAPVS"
BASE COUNT         1649 a         2111 c         2180 g         1760 t
ORIGIN      
        1 ctaaccgtca tggagataga cgttcacggt ttcaagggtg ccgacgaggt gacctattcg
       61 tggtacctgc tggactactt tgacgtgaag acctggaata catcgatgag ccgctgcaca
      121 gggtgctcct gcgccatctt cgccagggcg gtggcaaagg ggtatgtgaa gcagaagggc
      181 gtcttaccgg ccgaaaaact tgccgctgac gacgatcttt acaactatgt cattgaagag
      241 cagcaaaaga ggaggatcaa gtacaccgcg tcggtcaaga gagtgatgaa cgtaagatag
      301 ctttcttctt cactaagatc ctttttctcc tgcagggagg gggaggagcc ttcttcctcc
      361 tcccctcctt ccagaaggtc cctcgaggac aaaaacatct cccaagtctg attaaaatat
      421 gaaaatcatg tataatgacc ttgagcattc cggcagcccg tatcacttgt cttgagcgga
      481 agactgccgg ccgaccgggt gggtattcac aggtcgtaca aagggatgaa agggttttat
      541 ctgaagatcc cttcaagaag gaggtggggc ctgggcgtga tctcccgggt ttctttgtgt
      601 caccatgaac ttagactcaa atccttggag aaggaggttc tttcatgtcg gagaaacagc
      661 aagtcaagcc aagtctgggt ctctccatcg gcgtgttcgt ggcagcgacc gtcattatca
      721 gtttcggtgt tcttaaactg ggcgtagatg cccatatccc catcgttttt tccgctgtcc
      781 ttgtttgtat tgtgggcctt acagtcctca agatgccgtg gtcccagata gaggaaggcg
      841 gcctcaacgc aatagcagtt gcccttcagg cggtcgtcat acttatgata attggaatgg
      901 tcattggcat ctggatccag agcggagtcg tacccagcct tatctactac ggactttcca
      961 tcctgtcgcc ttcgatcttc ctgctggcca ctcttctgat cacgtccata gtctctatct
     1021 cgaccggctc ttcatggacc actgccggga ccgtaggtat agccctcatg ggcatagctc
     1081 acggtcttgg gatccccgcc ccgcttacgg cgggcatagt ggtctccgga gcctatttcg
     1141 gcgacaagat gtcgcctctt tcggatacga caaaccttgc gccggccgtt gcaggggcaa
     1201 acctttttga ccatatccgg gcaatggtct ggtccactgg gccgacctat cttatcgtag
     1261 cggcgatctg cgttttcctg ggcatgagat atgcgggcgg ggcgcttgac gcagagaaga
     1321 tctccgccat gcagcagatg atgaaggccg agttcaacat cgggcttctg gggttccttc
     1381 ccccccttct tgtaatcggg cttgcggtga ggaaggcccc ggccatcccc ggtcttttcg
     1441 ccggtgttct ggtggcggcg atcatgtccg ctgtccaggg taacggcatc ggcgacatca
     1501 tcgatgtgat ccactacggc tatggtgcct ccgtctccgg cgagttcgct ggtgccgagg
     1561 gcgaggctct gctcaacatg ctcagccagt tcaaccttga catcgctccg gaaatggcga
     1621 cagaggtcgg gggcatgctc agtgagctgg tctcaagggg cggcctcgat tcgatgatgt
     1681 ggaccatctc gctgatcttc tgcgccctct tctttggtgg tgtcatggag gcctgcggtt
     1741 tcctggagac catagtcggt tccatcacca gacaggtcaa gaccgtcggc ggaatgatgg
     1801 gttccgtcat cggtacctgc ttcctggcca acctcttcct cggggaccag tatcttgctc
     1861 tggtcatccc cggcaggatg ttcaagactg ccttcgagga gaagggcctt gcgcccagaa
     1921 tgctctcccg tgctacagag gatgcgggaa ctctcacttc ggcgctgata ccatggaaca
     1981 cctgcggagc tttccagagc agtgtcctgg gggtcgcaac gctggcctac gccccctatg
     2041 ccttccttaa ctacctcaac ccgatcgtct ccattatcat gtcctacatg aagataggca
     2101 tctactggag aaaagaggac ggaactgaca ttgtggcaaa ggagaagccg tagggaaagt
     2161 tttctttttg aaatagcggc tgtttttccg gcggcggggg aacccgccgc cggccctttt
     2221 gtgccctgac ccccttcatg gtttgacacc ccggaatccg aaggtgtagg ataaatatca
     2281 aaatgcatac aagtcaatac attatttcct tacaggaggg gagaatatgc gtatgcggga
     2341 caggaaagag atacctctct ggaagggtgt ctcggaacag gagtggaacg actggaagtg
     2401 gcaggttgcc aaccgcataa cgacagtgga aaagcttcgg caggtcatcg acatcaccga
     2461 tgatgaggca gccagtatcg gaaggagcct tggcaagctc aggatggcaa ttacgcctta
     2521 ctacgcatct ttgatggatc ccgatgaccc gaaatgccca gtgaggatac aggcggttcc
     2581 gactgagcac gagacgaagc tggccaaggc ggatcttcac gaccctcttc atgaggacgt
     2641 ggactcgccg acaccggggc tgacccaccg ttatccggac cggggacttc ttctgataac
     2701 tgatcagtgt agcatgtatt gccgtcactg caccaggaga cgtcacgccg gggagacgga
     2761 cagggcttac ggtgaagagc agatcaaaaa gtgcatcgaa tacgtcagga agacccctac
     2821 gttcagggac gttcttcttt caggcgggga cccactgacg gtggatgacg acaggctgga
     2881 gtgggtcatt tcggaactga ggaagatacc ccacgtggac ttcgtgaggc tcgggacgag
     2941 gatgcccgtt gtctgccccc agagaattac cgacaagctc tgcaccatgc tccggaagta
     3001 tcaccccata tggctcaata cccagttcaa ccatcccaag gaagtgacca gtgagaccgc
     3061 tgaggcatgt gccaagctag cggacgccgg tatccccctg ggtaaccaga cggttcttct
     3121 gaaagggatc aatgactgcc cctacatcat cagggagctg aaccagaggc ttctgaagat
     3181 ccgggtcaac ccgtattaca tataccagtg tgacctttcc gagggaatag agcacttccg
     3241 gacgagcgtg ggtaagggta tccagatcat agagtacctg cggggtcaca caacggggat
     3301 ggcccagccg ctgttcattg ttgatgcccc cggaggaggc ggtaaaatac cagtggggcc
     3361 gaactacgtt gtaagccagt ccgacaggaa ggttatccta aggaactacg aaggcgttct
     3421 gacgacttat acggagccag acgacaacca gagccacccc gaagagctct accactggga
     3481 ggagtacact gcccggcaga ggcagctctc gagggagggc cttatcaagc ttttcgaagg
     3541 ggaacttctc tctctcgaac ctgcccatct cgaccgccgg gagaggaaca aataatcgga
     3601 aacaaagaaa ggcctattgc aggaggtgcg ttccttggag gagctgctgg tatcccgttc
     3661 cgtcacaccc ttccccaagt ggatgaagtg gatggtcctt cttgtgggga tccttctcat
     3721 aggggacgga atgcggagct tcatgttcca caagatactc gtaggagctg ttctggccta
     3781 tatttcaggg tatgacaaga ggatcgtgct ttctccggga gggatagtcc ggcagacaag
     3841 gacctggatc actacccact cgaccaccct tccctgggac gaggtgcagt acgtcaactt
     3901 tgcctacagg ggttcgaaga tgatgtgttt ctttgaaaag gacgttacag gcctcaaggt
     3961 gctctttgac aggaacgatg agcctgaggt caggaggatc ctggaacttt acattcccga
     4021 tgtggagact ggagtggtgg gggggagtta gacagttagg cgaaaagatg atgttgttac
     4081 tccttcaaaa aggtgtattc ttcacgaaga agaggcatca aacgttttcc catcataagt
     4141 gaccttgaga gggggagtgg aagggctctc cgggaaaggc cctgttttgg ggagtgtcag
     4201 gttgctgagg aagatttcgc tttttgaagg aggttctggt ctatggttga gagaacgcag
     4261 gtcaaaccgg gtcttggtct ttctttggca gtgttcgctg cagcagccgt aatgataagt
     4321 tatggtgttc tgaaacttgg cgtcgacgcg catgttccca tagtattctc agctgtactg
     4381 gtctgcatcg tggggctcac ggtactgaaa atgccttggt cccagataga ggagggtgct
     4441 ctcaacgcca tagctgtagc cctccaggca gttgtgatcc ttatgatcat aggcatggtg
     4501 atcgggatat ggctccagag cggtgtggtc cccagcctga tctactacgg tctgtccatt
     4561 ctttcgcctt caatcttttt gcttgccact ctcctgataa cgtcgatcgt ctccctgtca
     4621 accgggtctt catggacgac cgccggcacc gtgggcattg cgcttatggg catagctcac
     4681 ggcctgggga tcccggcccc gctgacggcc ggggtggtta tttcgggggc ctacttcggc
     4741 gacaagatgt cccctctttc ggataccacc aaccttgcac cggcggtagc aggttccaac
     4801 cttttcgatc acatcagggc catggtatgg tccacgggac ctacctacct gatcgtcgca
     4861 gttatctgca tagtgatcgg aatgaagtat gcaggcggca cccttgatgc cgagaagatc
     4921 tccgcaatgc agcagatgat gaaggccgag ttcaacatcg ggcttctggg gttcctcccc
     4981 cccctccttg tcatagggct cgcggtgatg aaggcccctg ccatcccggg actattcgcc
     5041 ggggttctgg tagccgccgt catgtctgca gtacagggca acggtctggg ggacatcatc
     5101 aatgcgatcc actacggcta cgaggccacc tttgcctctg aggtggccgg agcagagggt
     5161 gaagccctgc tgaacatgct cagccaggcc gatctgagta ttgcccccga gatggccacc
     5221 gaggtcgggg gacttctcac agaccttcta acccgcggtg gtctcgactc catgatgtgg
     5281 tccatctcac tggtcttctg cgctcttttc tttggaggag ccatggaagc ctgcggattc
     5341 ctggagacca tagttggctc tatcaccagg caggtgaaga ccgtcggtgg gatgatgggg
     5401 gctgtaatag ggtcgtgctt cgtggccaac ctcttcctgg gagaccagta cctctccctg
     5461 gtcatccccg gaaggatgtt caagtcctca ttcgaggaga agggtcttgc gccgagaatg
     5521 ctatcccgtg cccttgagga ttcgggtacc ctgacctcgg cgcttattcc ctggaacaca
     5581 tgcggggcct tccagagcag cgtcctgggt gtcgctactc ttgcctacgc cccatatgcg
     5641 ttcctgaact atctgaaccc catcgtatca ctggtgatgt cctacctgaa gataggcatc
     5701 tactggaggc aggaagacgg taccgacgta gttgccaaga aaaagccgta gtttgcatcg
     5761 atcagcaagg tctgcattat ctgaccagat ccaggcgaag gggaaaacct catcagcttt
     5821 cccccttcgc ctttttcctt tcagtccccg agcttctcct ccacagatct gaccttgctc
     5881 ttcatggaga tatccttgtc ccgtctttcg tctatcctca gggtggtgcc cacacggaga
     5941 gccccctggt cgaaggggat ctcgtgggcc gctcttaccg cccgcagtac cacgtcaagg
     6001 tcaccttcca gaatggtccc catcggtgtc agcatagttt cgagcccgta ctgcctgagg
     6061 gtcttctcga cctcggcgac atagccgcta aggcttgtgg agcctgttcc atgtggaacg
     6121 atgaccactt cggcaaccac ctttcccatt gcggatccct cctcttacgt ggccgttttc
     6181 ccggcctgaa ctcttcaagt ataatcaatc cagtcaggaa gctgcagcaa aacatctcaa
     6241 cgagggggac ccggtaatgg agtgggagtt cgcccagaga aaccttggaa aacttgtcat
     6301 gcccatactg gtcatcctgg ggtggtacat tgccgacagg atcgtggtcg atctgacgcg
     6361 aaagctcttc gagaagggga tatccagggt cgagaacggc cagaccgggc accttgaggg
     6421 ccatgaaagg gcgtccagga tcaaacggct tgagacacta aagggtctgg tgacggatct
     6481 tttgcgttgg gggctcggtg ccgttgcttt cctgaccctt ctcagcacta tcaatgtgaa
     6541 tataatgccc attcttaccg gactcggtat tgccggactt gccatatccc tggcagccca
     6601 gaacattatc agggacttcc tgaacggtat attcgtggta gtggaggacc actacgccgt
     6661 cggcgatgtg gtgaagatag gagaatattt cggtgttgtg gagtatttct ctctcaggac
     6721 cacccacctc tccgaccttg acgggaacta tataataatt cccaacagca ggatcacaga
     6781 gcttgtgaac gcgaccaagt actggtccca ggcacaggtc gtggtaggtg tctcatacga
     6841 cacggatatc cggaaggctc tgggcgtcat ggagatagtg ggaagggaca tcaggcaaga
     6901 cttccccgac aaggtgaggg atgaccccca ggtccagggt atcctctcct tcgacgcaag
     6961 ctccatatcc ctgagggctc tcatccggac cttccccggg gaacactggt tcatcggaag
     7021 agagtatcgc cttcgcctca aggatgcctt tgacgccgag ggaataacga tcccgttccc
     7081 ccagatggat gtctggatcc gttcccccga gggcttcagg ataacaggtc agaagacgag
     7141 cgcgtagggg acaggcctga cgatacctcc gggcggcgag gtcacgatct ttcccatcca
     7201 ccagatagac gacgaacccc ctccgtccag gttaagggcg tccaccaggc cgagcctctc
     7261 agccactgcc gcagtctcga caatggtcgc cccgttgctg tgccagggat ccctcccgtc
     7321 gatgaccacg aaccagacct tctctccgtc gctccctacg aaggacctcg gatgggggac
     7381 agaaagggtc ctggggctaa acccctcggg gtcgaagaca cgggtccctt tttttatcag
     7441 catcggcccc gcctgaagaa catgatcgag ccccctgaaa ccctggtcta cccattgggt
     7501 cctctaagac acaggggccc ccgggggcgt gttccttagt tgttcgccgg cgactccacg
     7561 ggcaattacg aggaagccct ctctggtcac gaagtggttc gatgtcgaag agtgtcttac
     7621 cccggagacg accccttctt ttacggtcac ctcccagccg tcgacagggg ctgccgtgac
     7681 atagctccat acatcgggtg
//
LOCUS       DAOPKQ010000041         7683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10163_length_7683_cov_4.588490, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000041 DAOPKQ010000000
VERSION     DAOPKQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10163_length_7683_cov_4.588490"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK146_02445"
     CDS             complement(<1..297)
                     /locus_tag="PK146_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPE91130.1"
                     /translation="MSSSGSRMVRHSLRMMAGTFLSRILGLAREVLTAALFGATRQLD
                     AFLVAYTLANLSRRLLAEGALSAAFVPEFSRSLEKDGPEAARSLARQTLAVLLAA"
     gene            324..824
                     /locus_tag="PK146_02450"
     CDS             324..824
                     /locus_tag="PK146_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPE91131.1"
                     /translation="MRLEVREQMKVPTFVWGVGNEIMGDDAAGICAARMVMERDLPWI
                     RVFLCGVLPENHMTPLREAPPERLLIMDAADFGGEPGDIRLLETRDVAGAVLSSHGIP
                     IGVLLAPFEEKIFIRIIAIQPLYTGLREGLSPPVEKAVRKAAAAVCSGTWDRFPPLHS
                     ENLSPR"
     gene            complement(797..1525)
                     /gene="lptB"
                     /locus_tag="PK146_02455"
     CDS             complement(797..1525)
                     /gene="lptB"
                     /locus_tag="PK146_02455"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPE91132.1"
                     /translation="MSNRFLWARSLSKSYKGRAVVSSVSLKVKQGEIVGLLGPNGAGK
                     TTTFYMVLGIVRPDSGRILIDNEDLTGQPMYIRARRGIGYLPQEASVFRSLSARENIE
                     LVLEERGKERKNISRMASDLIEEFGLGDVEDLPGFALSGGERRRVEIARSLAMEPSFI
                     LLDEPFSGIDPIAVYDIQQIILGLRTRGYGILLTDHSVRETLAITDRTYLIHRGQILV
                     EGTPSEVADSDAARKFYLGERFSL"
     gene            complement(1522..2184)
                     /locus_tag="PK146_02460"
     CDS             complement(1522..2184)
                     /locus_tag="PK146_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HPE91133.1"
                     /translation="MNMRTAFAVSIAALLAAATVVTGAEQMRLTSDTLQYDPSRGAVV
                     ASGNVKVVGRGAEITSDEGEFDSEGMRSWFRKDVLALWPEGDLSMNCDELIVEDSSRG
                     QKITARRVTRFHDPVRNVTIRADVVEGTVAGGVFSDFVARGNVVADAVASGGEPTRVS
                     GQISQYSRARGTLEFSGEAVAVQKNRRITADTFIIYLQSGKIEAVGNPRMVVDLPPQE
                     AQ"
     gene            complement(2282..3292)
                     /locus_tag="PK146_02465"
     CDS             complement(2282..3292)
                     /locus_tag="PK146_02465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPE91134.1"
                     /translation="MRVAVLGATGAVGQEMLATLESRKFPVSDLVLLASRRSAGARVN
                     YGGCEIVVREVCPGAFEGVDIALFSAGGEASRIWAPKAIERGATVIDNSSAWRMEPDV
                     PLVVPEVNPGALAGHRGLIANPNCATIQAVMVLNPLHKVAGLEGFTAVTFQSVSGTGK
                     EAIRELASNSEALLSGEKGMSSVYPHDIAFNVLPHIGGFDEDGISEEEWKMIRESRKI
                     MGLPGLKVNCTATRVPVFRGHSEALCAVFRNPLSPDEARAILEAAPGVCLMDDPGSYR
                     YPTARNSKGSGSVFVGRIRKDTFWEMGLMMWVVSDNLLKGAALNAVQIAEMIQGEGIC
                     VS"
     gene            complement(3289..4506)
                     /locus_tag="PK146_02470"
     CDS             complement(3289..4506)
                     /locus_tag="PK146_02470"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPE91135.1"
                     /translation="MKIEVLKFGGSSLFGEGRIEAVAGRAAERFRNGVAVAVVVSAVG
                     HTTDSLMETANGLTTRLNRREMDQLISTGEQQSVALLTMALGKLGIPARSFSGWQNGI
                     TACGVFGDGRISSVNPRIVAGSLERGEVAVVAGFQGVTEEGDVITLGRGGSDLSAVAL
                     AAALEADSCRIYTDVEGIFSADPRVVPKAFRLERISCGECMEMAAAGARVLQARSVEL
                     AWRYEVPVFVGSSFERERGTWIMREDVSEGFVVRSITHDTGVAKVAVLGVPDTPGIAA
                     RVFSPLSEAGVGAEMIIQSVMRGQVNDIAFLVRKEFLGEAIDICRGTARDIGAQGVVF
                     DTEIGKISLVGAGLSNHPEAPARMFSILAAESVNIDMISSTSMSITCVVSSADVEKAA
                     VALHRVFIEGVPA"
     gene            complement(4503..5447)
                     /gene="thrB"
                     /locus_tag="PK146_02475"
     CDS             complement(4503..5447)
                     /gene="thrB"
                     /locus_tag="PK146_02475"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPE91136.1"
                     /translation="MNGQVFRIRVPATTANLGSGFDTIGLALSLYNVYDVFDLDEPGA
                     YRMEVIGEGSAELSDPESNMIIQSYEKACDMWKMTCPGFTLRCLNAIPLCRGLGSSSG
                     AVAGGVMIANSFRKDPLGMEDLLPLMVEIEGHPDNIVPACLGGLVVSGVGDGRLRYVK
                     LPPPPGDIFAVVAVPDVRVRTEDARNALPKEVSMGDAVFTLNRAAILAASWAAGKWEN
                     LAWAMDDRLHQKFRAKLFPGGEVILEKVKNVPGCLGAAISGSGPSVLAFARGAPRGVA
                     EAMCRVFREHGVRSRFFVLDVDEEGATLKKLARESVCQ"
     gene            complement(5444..6484)
                     /gene="thrC"
                     /locus_tag="PK146_02480"
     CDS             complement(5444..6484)
                     /gene="thrC"
                     /locus_tag="PK146_02480"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPE91137.1"
                     /translation="MRGILIRYRHLLPLTSKTPLVSLEEGSTPLIPLDSTGKDLGIRL
                     FAKFEGANPTGSFKDRGMVLAVAKAIEAGARGLVCASTGNTSASAAAYAARTGIPCTV
                     LLPGGKVAMGKLAQAMVYGARVISLKGNFDRALELALEVSRELGLAVVNSVNRYRLWG
                     QRSVAWEICDELGHPPDVVVLPVGNAGNITASWAGFVQYSRIGKIGRIPAMFGVQAEG
                     AAPLALGKPCPEPETVATAIRIGRPVNGKKARWAVRMSGGRFLSVSDGEILDAQKRLA
                     ACEGVFAEPASCAGIAGLYRLASQNGLSTGKEYVAVLTGNGLKDTDAATSGKRPPVEV
                     DAELKAIREVLL"
     gene            7276..>7683
                     /gene="greA"
                     /locus_tag="PK146_02485"
     CDS             7276..>7683
                     /gene="greA"
                     /locus_tag="PK146_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPE91138.1"
                     /translation="MADVFVSDHIVMTREGYEKLKAELVALRSEGRQEIARMLEDARG
                     FGDLSENAEYHSAKEAQGKLETRIQWLEYQLSKAKVVEPGDIDPTRVGLGTTVTLLDL
                     DGDNKLFYTIVGSEESDPAANKISASSPVGQAVI"
BASE COUNT         1621 a         2428 c         2004 g         1630 t
ORIGIN      
        1 ggccgcaagt agcacagcaa gggtctgtct cgccagtgat cgggccgcct cgggaccgtc
       61 cttttctagg gacctggaaa actcgggtac aaatgctgcc gacagggcac cctcggcaag
      121 aagcctcctg gagaggttgg ccagggtgta ggcaacgagg aaggcgtcca gctgccgggt
      181 ggccccgaag agagccgccg tcaggacctc gcgcgcgagg ccgaggatcc tgctcaggaa
      241 ggtccctgcc atcatcctga gggagtgacg gaccatcctg gatccgcttg aactcacgtc
      301 aatgcaccag cctttgtcga tagttgcgac tggaggtgag ggagcagatg aaggtcccga
      361 ctttcgtttg gggggtaggc aacgagatca tgggggatga tgccgctggc atctgcgccg
      421 ccaggatggt catggagaga gatctcccct ggatccgtgt cttcctctgc ggcgttctcc
      481 cagagaacca catgacccct ctcagggagg cccccccgga gaggcttctg ataatggacg
      541 cggccgactt cgggggggaa cccggtgata tacgccttct ggagacaagg gacgtagctg
      601 gagcggtcct ttcctcccac gggatcccca tcggggtcct tctcgccccc ttcgaagaga
      661 agatcttcat caggatcatc gccatacagc ccctgtacac ggggctccgg gaaggtctct
      721 cgccgcctgt cgaaaaagcg gtgagaaagg cggcagcggc cgtctgctcc ggaacatggg
      781 accggttccc tccccttcac agcgaaaacc tttcccccag gtagaatttc ctggcggcat
      841 cgctgtcagc cacctctgac ggggtcccct cgacgagtat ctgccccctg tggataaggt
      901 aggtcctgtc agttatggca agggtctccc tgacgctgtg atctgtaaga agtataccgt
      961 atcccctcgt tctcaggccg agtatgatct gctgaatgtc gtagacagct atcgggtcga
     1021 tgccgctgaa gggttcgtca agcagtatga atgagggctc catggcaagg ctgcgggcta
     1081 tctccaccct cctgcgctcc cctccgctca gagcgaagcc gggaaggtcc tccacgtcac
     1141 cgagaccgaa ctcctctatc agatccgagg ccatccggga gatattcttc ctctccttgc
     1201 ccctctcctc caggacaagt tctatgttct ctcgggcgga caggctcctg aagactgagg
     1261 cctcctgggg gaggtaacct atccccctcc gggcccttat gtacatgggc tgccccgtaa
     1321 ggtcctcgtt gtcgataagg atcctgcccg agtcaggcct tactatcccg agcaccatgt
     1381 agaaggtggt ggtctttccg gcaccgttgg ggccgagaag gcctactatc tccccctgct
     1441 ttaccttcag gcttaccgag gatacaacgg cgcgcccctt gtagctcttg ctcagcgacc
     1501 gggcccagag gaagcggttg ctcattgggc ctcctggggg gggaggtcta ccaccatacg
     1561 ggggttgccc acggcctcga tcttcccgct ctgcaggtaa atgatgaagg tgtctgccgt
     1621 gatcctgcgg ttcttctgta cagccaccgc ttcgccggag aactccagag tcccccgggc
     1681 cctggagtac tgcgaaatct gtccggagac cctcgtgggc tcacccccgg aggctacagc
     1741 atcggcaacc acgttgcccc ttgccacgaa gtcggagaag accccgcctg ccactgtacc
     1801 ttccaccacg tcggcgcgga tcgtcacgtt cctcaccggg tcgtggaacc ttgtcacccg
     1861 cctggcggtg atcttctgac cacgggaact atcctccacg atcagttcgt cgcagttcat
     1921 ggagagatca ccctcgggcc acagggccag gacgtctttg cggaaccagg agcgcatccc
     1981 ctcggaatcg aactccccct cgtcggaggt tatctccgct cccctcccga cgaccttgac
     2041 gttccccgag gccacaacgg cacctctgga gggatcgtac tgaagagtat cggaggtcag
     2101 ccgcatctgt tccgcaccgg tcactaccgt cgcggccgcg agaagggctg cgatcgaaac
     2161 tgcgaaggca gttctcatat tcatgatctc aggcaccatc ctaggaaaag aaatggcggc
     2221 acctcaaagg cgccgccgtt attctacctt aaactctgcc cgcttcgata gaaggaccct
     2281 gtcaggaaac acaaattccc tctccctgga tcatttctgc tatctgaaca gcgttcagag
     2341 ccgccccttt aagcaggttg tcggaaacca cccacatcat aagacccatt tcccaaaaag
     2401 tatccttccg gatcctcccc acaaaaactg atccacttcc tttcgagttc ctcgcagtag
     2461 ggtaccggta actccccggg tcgtccatca ggcagacccc cggggccgct tcaaggatcg
     2521 ccctcgcttc atcgggagag aggggattcc ggaaaactgc gcacagggcc tcggaatgcc
     2581 ccctgaaaac cggaactctt gtggcagtgc aattaacctt aagaccggga agccccatga
     2641 tctttctgga ctccctgatc attttccact cttcttcact gataccgtcc tcgtcaaacc
     2701 cgcctatatg gggcagaaca ttgaaagcta tgtcgtgggg atataccgaa gacatgcctt
     2761 tctccccgga tagtaaggct tcggaatttg atgccagttc cctgatggcc tctttccctg
     2821 ttccggaaac ggactggaag gttaccgctg tgaagccttc cagtccggca accttatgca
     2881 aaggattcag aaccataaca gcctggatgg tggcacagtt ggggttggcg atcaatcccc
     2941 tgtgccccgc aagagcacca ggatttacct cgggtaccac gagagggacg tcaggttcca
     3001 tccgccaggc tgaactgttg tcgatcactg tcgcgcccct ctctatggcc tttggtgccc
     3061 atattctgga tgcttcgccg cctgccgaaa aaagggctat atctactccc tcgaaagcac
     3121 cggggcaaac ctccctgaca acaatttcac accccccata atttaccctt gctcctgccg
     3181 agcgtcggga ggccagtaac accaggtctg agacggggaa cttcctgctc tcgagggttg
     3241 caagcatttc ctgccctact gccccggtag cgcccagcac cgccaccctc atgcaggaac
     3301 cccctcaatg aataccctgt gcagggccac agctgccttc tccacatctg ccgaggaaac
     3361 cacgcaagtt attgacatcg aagttgaaga tatcatgtcg atattaacgg actcagcggc
     3421 aaggatcgag aacatccttg ccggtgcttc agggtggttc gaaagacctg cacccaccag
     3481 ggatatcttc cctatctccg tatcgaagac cactccctgg gcaccaatat cccgggcagt
     3541 tcccctgcat atatcaatag cctccccgag gaattccttc ctgaccagga aagctatgtc
     3601 gttaacctgg cccctcataa cgctctggat tatcatctcg gcgcctacac cggcttcgct
     3661 caagggcgaa aataccctgg ccgctatgcc tggggtatcg ggtaccccca gtactgccac
     3721 ttttgcgacc cccgtatcat gggttatcga tctcacaacg aacccctctg atacatcttc
     3781 cctcatgatc caggtccccc tctccctttc gaaacttgat cccacgaaaa ccggtacttc
     3841 gtacctccat gcaagctcca cgcttctggc ctgcaaaacc ctggctcccg ctgccgccat
     3901 ctccatgcac tccccgcagc tgatcctttc gaggcggaag gcttttggaa cgacccttgg
     3961 atcggcagag aatattcctt cgacatcggt atagattctg cagctatcag cctcgagcgc
     4021 ggcggcaagg gctacggctg agagatccga cccgcctctg ccaagggtaa taacatcacc
     4081 ctcttcggtc actccctgga accctgccac gacggctact tcgccccttt caagggaacc
     4141 ggcaacgatc cgcgggttca ccgaactgat gcgcccgtcg ccaaaaaccc cgcaagccgt
     4201 tattccgttc tgccagcctg agaaagagcg cgcggggatg cccagtttgc ctagcgccat
     4261 ggtaagcagg gccacacttt gctgttcccc tgtcgatatc agctggtcca tctctctccg
     4321 gttcagcctg gtagtcagac cgttggctgt ttccatcaga ctatcggtgg tatgacccac
     4381 agcggaaacc actacagcta cagcaacacc attcctgaag cgttcggcgg ccctccctgc
     4441 cacagcttct atccgcccct cgccgaaaag tgatgacccc ccgaatttaa ggacctctat
     4501 cttcattggc agacactctc cctggcaagt ttcttgagag tggcaccctc ttcgtcaaca
     4561 tcaagaacaa aaaaacggga tctcacacca tgctccctga ataccctgca cattgcctcg
     4621 gcgacccccc ttggggcccc cctggcaaaa gcaaggacgc tgggtcccga cccgcttata
     4681 gcagcaccaa ggcacccggg aacattcttt accttctcaa gtatcacttc cccgccgggg
     4741 aagagtttag ccctgaactt ctggtgaagg cggtcgtcca tagcccaggc aagattctcc
     4801 cacttcccgg cagcccagga tgcggccaga atggcagccc tgttcaacgt gaagaccgca
     4861 tcccccatgc tcacctcttt gggcagggca tttcttgcgt cctcggtcct gacccggaca
     4921 tccgggactg ctactacggc aaagatatca cccgggggag gaggaagctt gacatacctc
     4981 agtcgtccat cccctacacc gctgacaaca aggcccccga ggcatgccgg aactatgttg
     5041 tcgggatggc cctcaatttc gaccatcagg gggagaagat cctccatccc cagggggtcc
     5101 ttccggaatg aattggctat cataactcct ccggcgacag ctccggagga acttccgagc
     5161 cctctgcaca ggggaatggc gttcaggcat cggagagtga aaccgggaca tgtcattttc
     5221 cacatatcgc atgccttttc gtaactctgg attatcatgt tcgactccgg atcggagagt
     5281 tctgcgctcc cctcgcctat tacttccatc ctgtaggccc ccggttcgtc cagatcgaaa
     5341 acgtcataaa cgttgtagag ggacagagcc agaccaatgg tgtcaaaacc cgagcccagg
     5401 ttcgccgtgg ttgccgggac ccggatacgg aagacctggc cgttcataag aggacctccc
     5461 tgatggcttt cagttcagca tcgacctcta ccggcggcct tttcccggaa gtggcggcat
     5521 cggtgtcctt cagcccgttg cccgtcaata cagccacgta ctcctttccc gtcgacagcc
     5581 cgttctgtga ggcaagccgg taaagacccg cgatgcccgc acatgatgcc ggctcggcaa
     5641 agacgccttc acacgccgcc aaacgtttct gggcatcgag gatctccccg tcgctgaccg
     5701 aaaggaacct gccgccactc atgcgaacag cccaccgggc ctttttgccg ttcacaggcc
     5761 tccctatacg tatggccgtt gcgactgtct ccggttcagg gcaaggttta ccgagggcaa
     5821 gcggcgcggc gccctcggcc tggacaccga acattgcggg gatacggccg atctttccga
     5881 tccgggagta ctgtacgaat cctgcccagc tcgctgttat gttccctgcg tttcctaccg
     5941 gcagcacgac aacatcaggg ggatgcccga gttcatcaca tatctcccac gcgacgctcc
     6001 tttgacccca gagcctgtag cggttcaccg agtttacgac ggcaagccca agttcccggg
     6061 aaacctcaag ggcaagttca agggcccggt cgaaattccc cttgagggat ataaccctgg
     6121 caccgtagac catggcctgg gcaagttttc ccatggccac ctttccgccg ggaagaagaa
     6181 cggtacaagg gatgcctgtt ctcgcagcgt aggctgccgc cgaggcagag gtgttccccg
     6241 ttgaagcgca gacaagcccc ctggcgcccg cctcaatggc cttggcgaca gccaggacca
     6301 tccccctgtc cttgaaggaa cctgtcgggt tcgccccctc gaacttcgcg aacagtctta
     6361 tccccagatc ctttcctgtc gaatcaagag ggatcagcgg cgtactcccc tcctcaagcg
     6421 ataccagcgg cgtctttgag gtcaatggaa gcaggtgcct gtaacggatc agaattcccc
     6481 gcatgggaaa ccctcctcaa attaactgca aataaaaagc ctcttttcat ctccatgtcc
     6541 aggagacgaa aagaggctct tttcgcggtt ccactccggt tccagccaaa catcagccgg
     6601 ctctcgtacc cgctgtaagg ggcggggccc cgtcacctta tccctttcag ggcttcggat
     6661 ccggctccag ggtggtcttc accgctccct tcgcgagggg atctttcagc cgcggatccc
     6721 cctctcttgc gcgccgtgga acggttactc tccctgtcat cgcctttgcg gtatatacct
     6781 ctccggtttc cgaagtttat agacctcagg aaaggatttg tcaaggacaa accttaatcg
     6841 aaaaaacctt aagggcgtta acagtgaacg gtcaacggtg aacgaaaagc caaagatctc
     6901 tgtcatttcg ataggctgcc tcctggggga gactaagttc ccgaactgag tgaagggtgg
     6961 gggtctgttt tgattaaaaa attactcgga cctgatccaa taacagatcc ctcgtcgtct
     7021 aacaaacctt ctcgggatga cagaaatccg gttttcacta cccaggggtt tctgtcttta
     7081 ccgttgagcg tgtacgcctt tgatctgagg ttcccagacc tggtttctat tggatttttc
     7141 cgtttactgt ttaccgttga ccgtttacgc cttttagtat cccaggtttc ccctcccata
     7201 ttgcccattt cttgacggtc tgttaaaata cgccatcagc ggaagtcata ttccccccga
     7261 agggagggta cgtacatggc agatgttttt gtttccgacc atatcgtcat gacccgggag
     7321 ggatacgaga agctgaaggc agagctggta gccctcagaa gcgaaggacg tcaggagatc
     7381 gcgaggatgc ttgaagacgc ccgcggcttc ggggacctca gcgagaacgc ggagtatcac
     7441 tcggcaaagg aagcacaggg caagctcgag acaaggatac agtggctgga gtaccagctg
     7501 agcaaggcaa aagtagtgga accgggagat atagatccca caagggtcgg cctgggtacg
     7561 acagtgaccc tcctggacct ggacggcgac aacaagctct tctatacgat cgttggctcg
     7621 gaggagtcag atccggcggc aaacaagata tccgcaagca gcccggtcgg acaggccgtt
     7681 atc
//
LOCUS       DAOPKQ010000042         7670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10190_length_7670_cov_5.041366, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000042 DAOPKQ010000000
VERSION     DAOPKQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10190_length_7670_cov_5.041366"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK146_02490"
     CDS             complement(<1..357)
                     /locus_tag="PK146_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HPE91139.1"
                     /translation="MKLSQNATWYILRGFGFIVNRASHCLSVKLGEWIGLIVWLFSPA
                     RVDRAEARCVKVLQVGVTTARSIVKESYRNLGRGLAEVLRLPTLGSGIMNYVEVHGEE
                     NLQEALSKGKGVIGLTG"
     gene            451..1248
                     /locus_tag="PK146_02495"
     CDS             451..1248
                     /locus_tag="PK146_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPE91140.1"
                     /translation="MFRRILFPLDLSGLSRKGLAWTAGNVADPSSEIVLVHVVEPVSG
                     IDTGPLIDKAEKALDAIEAELHGMGIMSRSFVKTGDPLEILPQVAHSERCSISAVFAE
                     RDDVVVPSLRLMAIPHLVYKSDEGAMPPPDPFRQIAVATDLSPFRTNPVFRELRKLLA
                     GRDVPMTLLHSVSMDDPSTSTELFQVAASALEEVRKTIEKWNPGTTAEILGGEPEVEI
                     LRTVKNIRPGMLVVGLSVHGHLWELIVGSTGEAIIEQAPCPVLVIPS"
     gene            complement(1259..2545)
                     /locus_tag="PK146_02500"
     CDS             complement(1259..2545)
                     /locus_tag="PK146_02500"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPE91141.1"
                     /translation="MRTFLSNEVKPALGCTEPGAVALAASWAAKHGGGQIRSIRLQLS
                     PNIFKNGLRAGIPGTEGGTGNLLAAALGALKGDPEKGLLALEGVTGQDVAAAMDLVDS
                     GAVTQTVMRDVPSVYVEMELIDDTGNITVIISGRHDRVVQVRKNGRIVKRLLEADTDS
                     GGRPPYIEELLDEDMEGLWRLAGDLKDGEAEFLLEGSRTNMKVAERGLEKPWGLGIGF
                     SLAALGGMENLALSVKAWSAAAADVRMAGGPWPVMSSAGSGNHGITAIVPPSVAAGAW
                     SRSDRELAEALALSHLVTGFIKAHTGRLTPVCGCAIAAGAGAAASMVRLGGGTPRQAE
                     ISVALLLSSLQGMVCDGAKGSCALKVGTAAAEASTSAMLVLKGLKDPGDQGMISSDFV
                     ATAKNVGDFSREGLMSADPAIIRILQGMDPASRDRS"
     gene            complement(2709..3833)
                     /locus_tag="PK146_02505"
     CDS             complement(2709..3833)
                     /locus_tag="PK146_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPE91142.1"
                     /translation="MGLYFRALAEIGEQSGFMGLTTGHIIMLVVALVLLYLAIAKGFE
                     PLLLVPIATGCLLVNLPLSGIMDEGGFLRYLFYGAEHEIYPVIIFMGIGALTDFSPLL
                     ANPVTFLLGAAAQLGVFVALFGAMMIGFSVPEAASIAIIGGADGPTSIYLTMKLAPQI
                     LGAVAVAAYTYMSLVPMVQPPIIKLFTTRKDRAIRMDALRPVSKRERILFPIVCTMAA
                     GLILPASVPLIGLLMFGNLLRECGVTERLSQAAQNEILNTVTILLGLSVGATMGAESF
                     LTTATIKIIILGLVAFIFSTAGGTLFGQLMKVLSGGKINPMIGAAGVSAVPMAARVVQ
                     RISARENPSNFLLMHAMGPNVAGVIGTAVAAGAMLTLLTN"
     gene            complement(3867..4265)
                     /locus_tag="PK146_02510"
     CDS             complement(3867..4265)
                     /locus_tag="PK146_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPE91143.1"
                     /translation="MSKKYKITVNGTAYEVEVEDIGSSASAPAPAPAAAPAPAPAAAP
                     APAPASAPAAAPAPAAGGGTTVTAPMPGKVLRVVTSVGASVNAGDLLLVLEAMKMENE
                     IQAPSAGTVKEIKVSDGSPVNTGDVLVVIG"
     gene            complement(4465..4857)
                     /locus_tag="PK146_02515"
     CDS             complement(4465..4857)
                     /locus_tag="PK146_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPE91144.1"
                     /translation="MELSHIADKFSHGIGGAIALAMIAFSVVFLVLIALTLIIMGTRY
                     LADLSERKPMKPAGPEQKAVPAPVAPSAPAAGEGGDKNVIAAVIAAAIAASGGGRATS
                     VKPLVVTGRGKPSAWKTAGRMDLLEGFE"
     gene            complement(4870..6429)
                     /locus_tag="PK146_02520"
     CDS             complement(4870..6429)
                     /locus_tag="PK146_02520"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPE91145.1"
                     /translation="MSERTIGELCEALVRLRDEAEAGGGEKAIAKQHEKGKLTARERI
                     DKLLDDGSFVELDAFVEHRCIFFGMDRKAYPGDGVVTGYGTVEGRLVYVYSQDFTVMG
                     GSLGEMHAKKICKVMDLAMQNGAPVLGLNDSGGARIQEAVDSLSGYGNIFYRNTIASG
                     VVPQLSLILGPTAGGAVYSPALTDFIFMVDKIGILHITGPAVIKAVTGEDVTSEQIGG
                     AKAHNQISGNAHFFATSEDECFSQVRKLLSYLPSNNMEEPPFYDSGDDPYRCDDSLRD
                     IVPTNPNKGYRVQDVLSRVADGGEFFEVQPMWAKNMVTGFMRVGGYVAGIIANQAAIM
                     AGCLDIDASDKASRFIRYCDAFNIPVVTFVDVPGYLPGKDQELGGIIRHGAKLLYAYS
                     EATVPLITVVLRKAYGGAYLGMCSKDLGADVVLAWPQAEIAVMGAEGAANIIFKKEIE
                     SAQDPQATRTQKIEEYREAFATPYQAAKRGFVDRVILPEETRGEIYRALVQCESKRQL
                     RPRRKHGVMPH"
     gene            complement(6478..6882)
                     /gene="mce"
                     /locus_tag="PK146_02525"
     CDS             complement(6478..6882)
                     /gene="mce"
                     /locus_tag="PK146_02525"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPE91146.1"
                     /translation="MKPTAIDHIGIAVTSIDEALKFWEQSLGIKCTGVEEVAEQKVKT
                     AFLPLGDTEVELLEATDPESPVAKFIEKKGEGIHHLAIRVQNLEEALEKMNALGLRMI
                     DEKPRYGAGGARIAFVHPRTAGGVLLELSERP"
     gene            complement(7012..7428)
                     /locus_tag="PK146_02530"
     CDS             complement(7012..7428)
                     /locus_tag="PK146_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPE91147.1"
                     /translation="MSERRIRVIVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     AESIVETAIQEDADAIGISILSGAHEHYFSEIIKLLKEKSAEDIVVFGGGVIPEGDIP
                     GLKEMGAGAIFGPGTSTSTCIEWLEEKVREKRSKES"
     gene            complement(7457..>7670)
                     /locus_tag="PK146_02535"
     CDS             complement(7457..>7670)
                     /locus_tag="PK146_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPE91148.1"
                     /translation="NQEKALKAMKQGRDNVLVGNRLDEVKAAARDENLNLMPAMIDAV
                     KVYATEGEICGVLREEFGEYRENIVL"
BASE COUNT         1530 a         2354 c         2078 g         1708 t
ORIGIN      
        1 gccggtcagg ccgatgacgc ccttgccttt cgataaggcc tcctggaggt tctcctctcc
       61 gtggacctct acgtagttca ttatccccga acccagggtg ggcagacgaa ggacctcggc
      121 caaacctctc cccaggttcc tgtaggactc tttgactatg gatctcgctg tcgttacacc
      181 tacctggagc actttcacac accttgcttc ggccctgtcc accctggctg ggctgaaaag
      241 ccagacaatg agtccgatcc actctccgag cttaacagag agacaatggg aggccctgtt
      301 cactataaat ccgaatcctc ttagtatgta ccaggtggcg ttctgggaaa gtttcaacag
      361 ctgtcctccc tgtgctgacc tgtccttgac tgcaaaacaa ggtctggtaa actgatttag
      421 tctagcacaa aattaaaggg ggcatccgtc atgttcagaa ggatactctt cccgttggat
      481 ctctccggcc tttcccggaa gggccttgcc tggaccgccg gcaacgttgc cgacccttcg
      541 tcggagatag ttctggtcca cgttgtcgaa cccgttagcg gaattgatac gggacccttg
      601 atagacaagg cagaaaaggc tcttgacgcc atcgaagcag agttgcacgg aatggggatc
      661 atgagcagat cctttgtcaa gaccggtgac cctttggaga tactgcccca ggtggctcac
      721 tcggagagat gctccatatc cgccgtcttt gccgaaagag atgacgtggt ggtcccttct
      781 ctccggctca tggccatacc tcaccttgtc tacaagtcgg acgaaggcgc aatgcctcct
      841 cccgatccat tccgtcagat cgccgtcgct acggacctct cccccttcag gaccaacccc
      901 gtcttcaggg agctcagaaa actccttgca ggcagggatg tcccgatgac ccttctccac
      961 tccgtgtcca tggacgatcc ttccacatca acggagctgt tccaggttgc cgcctcagca
     1021 ctggaggagg tccgcaaaac catcgaaaag tggaaccccg gaactaccgc cgagatcctg
     1081 ggcggggaac cggaggtgga gatactccgc acagtcaaaa atatcaggcc cggcatgctc
     1141 gtggtggggc tctcggtcca cggacaccta tgggagctta tagtggggag caccggcgag
     1201 gcaatcattg aacaggcccc ctgcccagtc cttgtaatac cttcctgacc cttccctgtc
     1261 aggaacggtc ccttgaagca gggtccatgc cctgtagtat gcgtattatc gcaggatcgg
     1321 ccgacatgag cccttccctg ctgaaatcgc ccacgttctt cgctgttgca acaaaatcgg
     1381 aggagatcat cccctggtct ccaggatctt tcaggccctt caggaccagc atcgctgagg
     1441 tactggcctc ggctgcggcg gttccgacct tgagggcaca ggaacccttg gcgccatcgc
     1501 agaccatgcc ctgaagggac gagaggagca gggcaacgga tatttccgcc tgccttggag
     1561 ttcctccgcc gagtcgcacc attgaggccg cagccccagc cccggcggcg atggcacagc
     1621 cacagacagg agttaggcgc ccggtatggg ccttgatgaa tccagtaaca agatggctca
     1681 gggcaagggc ctccgcaagt tcgcgatcgg atctgctcca ggccccggca gctacagagg
     1741 ggggaactat tgcggtaatg ccgtggttgc cgctaccagc gctgctcatc acgggccagg
     1801 gaccgcctgc catccttacg tccgctgcgg ccgcagacca ggccttcaca gagagtgcaa
     1861 ggttttccat cccgcccagg gcggcaaggc tgaagcctat acccagaccc cagggtttct
     1921 ccaggccccg ttcggccact ttcatgttcg tcctggaccc ttcgagaagg aactccgcct
     1981 ccccgtcttt caggtcaccg gcgagcctcc agagcccctc catgtcctcg tccagtaatt
     2041 cctcgatata gggtgggcgt cctccactgt cggtatctgc ctcgagaagc cttttgacga
     2101 tccggccgtt ctttcttacc tggaccaccc tgtcgtgcct tcctgaaatg atcacggtga
     2161 tgttacccgt gtcatcgatg agctccattt cgacatagac ggagggaaca tccctcatca
     2221 ccgtctgcgt cacagcgcct gaatcgacca ggtccatggc ggcagctacg tcctggcctg
     2281 tcactccttc aagggctaaa agtcccttct ccgggtcgcc cttgagggca ccgagagccg
     2341 cagcgaggag attccccgtt ccgccttccg ttccagggat cccggcacga agaccgttct
     2401 tgaagatatt gggcgagagc tgaagtctaa tggacctgat ctggcctccc ccgtgctttg
     2461 cggcccagga ggcggccaag gctacagctc cgggttccgt acaacccagg gcaggtttga
     2521 cctcgttaga aaggaacgtt ctcatatcca atggagatac ctcccggaaa gtttttcccg
     2581 acgctacatt tttacctcaa aggccaacct gcaacaaggg cgataacacc tttgaaggga
     2641 taaaaaaggc gcgagaccca ggagatcccg cgccgacatg tctttccggg agcggacaga
     2701 tgatgggact agttggtcag aagcgtgagc atcgcgcctg ccgcaactgc cgtcccgatg
     2761 acccctgcca cgttgggtcc catggcgtgc atgagaagga agttcgacgg gttctccctg
     2821 gcgcttatcc tctggacgac cctcgctgcc attgggacag cggagacccc tgccgcacct
     2881 atcatggggt tgatctttcc gccagatagg accttcatca gctgtccgaa gagggtacct
     2941 ccagcagttg agaatatgaa tgccaccagt ccaaggataa tgatcttgat cgtggcggtg
     3001 gtaaggaaac tttcggcccc catggtggca ccgacggaaa ggcccagcag gatcgttacg
     3061 gtgttgagta tttcgttctg ggcggcctga ctgagccttt ctgtaactcc gcactcccgg
     3121 agaagattgc cgaacatcaa aagaccgata agtgggaccg atgctggcag gatcaacccg
     3181 gccgccatgg tgcagactat gggaaagagg atcctctccc tcttgctgac gggacggaga
     3241 gcgtccatcc tgatagccct gtctttcctg gtggtgaata acttgatgat cggcggctgg
     3301 accatgggca caagggacat gtaggtatac gccgccacgg caacagctcc aagtatctgc
     3361 ggggccagtt tcatcgtcag gtagatcgag gttggtccgt cagcacctcc aatgatggcg
     3421 atggaggccg cctcggggac cgaaaagcct atcatcatgg cgccgaagag ggccacgaat
     3481 actccaagct gagcagcggc acccagcaag aacgtcacgg gattcgccag aagaggcgaa
     3541 aagtcggtga gtgcaccgat acccatgaag atgatcaccg ggtagatctc gtgctccgcc
     3601 ccgtagaaaa ggtagcgcag gaagccccct tcgtccatga tccccgagag cggcaggttg
     3661 accaaaagac atcccgtcgc gataggtacc aggagaaggg gctcaaatcc cttggctatg
     3721 gccaggtaga gcaggaccaa agccactacc agcattatta tgtgtccggt agtgagtccc
     3781 ataaaaccgg actgttcacc gatctctgcc agggccctga aataaagtcc catttgaaaa
     3841 cctcccctgc aaggactttt gcagtcctag cctattacta caagaacatc gcccgtattc
     3901 accggggatc cgtccgatac cttgatctcc ttgaccgtcc cggctgaagg ggcctggatc
     3961 tcgttttcca tcttcatcgc ttccagaacg agcagaaggt caccggcgtt gacggatgct
     4021 cccacacttg tgacgaccct gaggaccttc cccggcatag gggcggtcac ggtggtgcct
     4081 ccgcccgccg ccggagcagg tgcagcggca ggagccgatg ctggtgccgg ggcaggagcc
     4141 gcagcaggag ccggcgcagg ggctgctgcc ggagcagggg ccggtgcgga cgccgaggat
     4201 ccgatgtcct ccacttcaac ttcgtaggct gttccattaa cggtgatctt gtattttttg
     4261 gacatggttt tgttttttcc tccttattta ttctgcaaaa aattttgatt cgaaattttt
     4321 tgaaattcat tcacctttcc aatatgaaag gcgtaaaccg tgaacggtaa acagtgaacg
     4381 gtaaaacctt gaacatagat cgaaataacc gtttaccgtt gacccagtgc ccggagcgga
     4441 agcgactggg tgccggttac ctttttattc aaacccctcc agcaggtcca tcctcccagc
     4501 ggtcttccag gccgatggtt tgcctctgcc tgttaccaca agaggtttga cagatgtggc
     4561 cctgcctccg ccggaggcag ctatggcagc tgctataacc gctgcgataa cgtttttgtc
     4621 gccgccttcc cctgctgcgg gagcggaggg cgcgactggc gccgggactg ccttctgttc
     4681 ggggccggcc ggcttcatcg gtttgcgctc ggagagatcc gcaaggtagc gggtccccat
     4741 tatgatcagg gtaagagcga tcagaacgag gaagaccact gaaaaggcga tcatggccag
     4801 cgctatggcc cctcctatac cgtgggagaa cttgtcggca atgtgggaaa gttccatcct
     4861 gatacctccc tagtgcggca tcacgccgtg cttgcggcgg ggacggagct gccgcttgct
     4921 ctcgcactgg acaagggccc ggtatatctc gccgcgggtt tcctccggga gaatgaccct
     4981 gtccacgaat cccctcttgg cggcctgata gggtgtggca aaagcctccc ggtactcctc
     5041 tatcttctgg gttctcgtgg cctgggggtc ctgggcgctc tctatttctt tcttgaaaat
     5101 gatgtttgca gcaccctcgg cgcccatgac ggctatctca gcctgtggcc aggcgaggac
     5161 cacatctgca cccaggtcct tgctgcacat gcccaggtat gcaccaccgt atgcctttcg
     5221 aaggaccaca gtgataaggg gcaccgtggc ttcgctgtat gcatagagca gcttcgcccc
     5281 gtgcctgatt atgcctccaa gctcctggtc cttccccggg aggtagccgg gaacgtcgac
     5341 aaaggtgacc accggaatgt tgaaagcgtc gcagtagcga atgaacctgc tagccttgtc
     5401 ggatgcgtcg atatccaggc agccggccat aatggcggcc tggtttgcga tgatccctgc
     5461 gacatacccg ccgacgcgca tgaaaccggt gaccatgttc tttgcccaca tgggctggac
     5521 ctcgaagaat tctccaccgt cagccaccct ggagagaaca tcctggaccc tgtagccctt
     5581 gttggggttg gtcggaacga tgtccctgag gctgtcgtca caacggtagg ggtcgtcgcc
     5641 actgtcgtag aagggaggct cctccatgtt attggaaggc agataggaga gcagcttcct
     5701 tacctgtgag aagcactcat cttcggaggt ggcgaagaag tgggcattgc cggagatctg
     5761 gttgtgagcc ttggcccctc cgatctgctc gctggtaacg tcctcaccgg ttacggcctt
     5821 aatgacggca ggaccggtta tatgcaggat gcctatcttg tcgaccatga atatgaagtc
     5881 ggtcaaggcc gggctgtaga ctgcaccccc agcggtggga cccaggatca gtgaaagctg
     5941 gggtaccaca ccactggcaa tggtgttgcg atagaagata ttgccgtatc cgctcaggct
     6001 gtcgacagcc tcctggatcc tggcgccgcc gctgtcgttc agccccagca caggggcccc
     6061 gttctgcatt gcaaggtcca tgaccttgca gatctttttg gcgtgcatct caccgaggga
     6121 tccacccatt accgtgaagt cctggctgta gacgtagacc aggcgtcctt ccacggtacc
     6181 gtaaccggtc accacgccgt caccggggta ggctttcctg tccatcccga agaagatgca
     6241 cctgtgctcg acgaacgcat ccaactcgac aaaacttcca tcatcgagaa gtttatcgat
     6301 cctctccctt gctgtcagtt tgcccttctc gtgctgcttc gcgatcgcct tctcgccgcc
     6361 gccagcctcg gcttcgtccc gtagcctgac caaagcctcg caaagctctc caatcgtccg
     6421 ctcagacatc tcttttcctc cctttgggca tattcccggc tccttgccgt gatacgacta
     6481 cggacgttcg ctgagttcca gcagaacgcc ccctgcagtc ctggggtgga caaatgctat
     6541 cctggcaccc cctgctccgt acctgggctt ctcgtcgatc atcctcaggc cgagagcgtt
     6601 catcttttcg agggcctctt ccaggttctg gacccggata gcaaggtggt ggataccctc
     6661 ccccttcttc tcgatgaatt tggccacggg gctttccgga tcagtcgctt cgagaagctc
     6721 cacctcggta tcaccgaggg gcaggaaggc cgtctttacc ttttgttccg ccacctcttc
     6781 gactccggtg catttgatcc ccagcgactg ctcccagaac ttgagagctt cgtcgatgct
     6841 tgtaacggct atcccgatat gatctatcgc tgtaggcttc atgctgtccc tccaataggc
     6901 gcgggggcac tgccccctga tattctgaac tcgctctctg gtcctttgca atggtaaccg
     6961 ataccaggct ggaacagggt tgcagatcat tcactgcctg ttcagttctt ttcaggactc
     7021 ctttgacctc ttctcccgta ctttctcttc aagccactct atgcaggtgc tcgtcgaagt
     7081 accggggccg aagatggctc cggcgcccat ctccttcaat ccggggatat ccccttcggg
     7141 gatcactccc ccgccgaata ctactatgtc ctcggcactc ttttccttca ggagcttgat
     7201 tatctcactg aaatagtgct catgggcacc gctcaggatg cttatcccga tcgcgtcggc
     7261 atcttcctgg atggcggtct cgacaatgct ctcagctgtc tgcctgagtc ccgtgtagat
     7321 cacctccatg cctgcgtccc tcagagccct tgccacaacc ttggcacccc tgtcgtgccc
     7381 gtcaaggccg ggcttggcca cgataacacg gatcctgcgt tcgctcatct tccgtccctc
     7441 ctgatccccg acgtccctaa aggacgatat tctccctgta ttcaccgaat tcttcgcgaa
     7501 gaacgccgca gatctcgccc tcagtggcgt acacttttac ggcgtcgatc atggccggca
     7561 tcaggttgag gttctcgtcc ctggcagcgg ccttcacctc gtcgagccgg ttcccgacga
     7621 gtacgttgtc acgcccctgc ttcatggcct tcaaggcctt ctcctgattc
//
LOCUS       DAOPKQ010000043         7632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10255_length_7632_cov_4.496899, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000043 DAOPKQ010000000
VERSION     DAOPKQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10255_length_7632_cov_4.496899"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PK146_02540"
     CDS             <1..554
                     /locus_tag="PK146_02540"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPE91149.1"
                     /translation="WGLKGEIAPDPVLAMVPGFQRMGDGDLLLVNIRPWHGGLPETTA
                     LASVSVQERCRLEMIAVALSDEDESLMSDLRSRGMFSPSRIVRIGGIEEARSLWRRAK
                     VAVGMRLHFCLLSALAGIPCLAVPYDPKVRFFAEDWGLPVWEGKLPSSFPGIGLNRSR
                     IEEASERLQEVFSRVLGSLLAGE"
     gene            559..1413
                     /locus_tag="PK146_02545"
     CDS             559..1413
                     /locus_tag="PK146_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPE91150.1"
                     /translation="MKASVRRELENVALEVRKDIVRMTGLSRSGHLRSCLSVVDINVY
                     LYWKVLRIRPEEPHWPERDRLVFSKNHACPALYAVLARRGFFEREELWNYRRLGAMLQ
                     GRPEHFRTPGVDAPGGSGGMGIGLANGLLMSARMGRLDRRVFCVMGDGELQVGAVWES
                     AMQAANLGSGALTTFVDMNGIQTGGSVESIKRIEPVRKKFEAFGWRVMEVDGHDFRQL
                     EKAVAWSLSGPGESPSAVLAKTVPGKGVTIAEKGLLGPPACPLDRDRMEQALRELESS
                     SGGRGGEV"
     gene            1416..2354
                     /locus_tag="PK146_02550"
     CDS             1416..2354
                     /locus_tag="PK146_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPE91151.1"
                     /translation="MRSTLSGFGEGLLGTGRKRNDVVLLHADMVDAEVITPFSLEFPE
                     RTINCGVSEQDMVMTAAGLSLGGKIPVAVSYSAFLTARSFDQIRTCLAIPGLQAVLVG
                     THSGLSAGEEGATHQMNQDVALMRSLPDMSVLVPSDYASSSAMAGIACGHPGPVYLRL
                     GRDEVPDIGILDESEMRIGGGRALVDGDGVTICACGIMVHEAMKAAAILSQQGINAEV
                     IDCFSLKPLPSQLILASLRRTGCCVVAEEHNIIGGLGEAIAALSASENPVPVRFVAVS
                     DRYGQSGTPSELQEYYGLTFREIVGESAQVWSLRRR"
     gene            2358..3053
                     /locus_tag="PK146_02555"
     CDS             2358..3053
                     /locus_tag="PK146_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE91152.1"
                     /translation="MEIRFAGVTKQFLPDIVALQDIFLTIEKGEFVYLVGPTGSGKTT
                     LLRLVTRELVPTKGRVTVGDVNIRKIASHNLAYLRRNIGVVFQDFRLLPDLTVYENIA
                     FVQEVIGVSSHDVQKRTNDVLDRLGLWRRRSLFPEQLSGGEQQRVAIGRAIANSPSIL
                     LADEPTGNLDIDTADEIMQLLLSINASGTTLIVATHNQYIVDSYRQRVIELRKGRLVR
                     DEKKGRYRVDGDF"
     gene            3040..3927
                     /locus_tag="PK146_02560"
     CDS             3040..3927
                     /locus_tag="PK146_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPE91153.1"
                     /translation="MGTFRYVMRDTWRLLSRHSGLSLLTIITSVAVFFLVGASILFVL
                     NTRYLVEAVEGDLTVQAYVRDSQEALEVVARKASSYDSVSSVRVVTSGEALERLKARL
                     GKLAEAVTLLDENPLPPSVEIQVKKASFATVIARELIAMPEVEEVVYAGTVAERLQKI
                     SHFVARLSIVVLVVSLASAALVLFNTIRIGVFARKEEISKMLLVGASRTFVVFPYVLQ
                     GVILGTAGAALSVLLLWFSYGMAITALERSLPFLRLLTDRIIVLRVGAVLVLAGVSVG
                     WICSWLAASRFVRQASRSI"
     gene            3951..5144
                     /locus_tag="PK146_02565"
     CDS             3951..5144
                     /locus_tag="PK146_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPE91154.1"
                     /translation="MKVKGNSSTVRSLLLVFSLLVLFPSRVSCAPDIDSRIKDEQGRL
                     ESIERQISYHQKQISEARRKEQGLLDELSKIDQSVTLLRQKVALLDLQREKTEMRIKE
                     LTSEIETTEAQLSRTKGYLARRFEAIYKYGGIAEFNLLLSAGSTHEALNNAYLLARIA
                     REDERRIVTMSENKKKLESAMKEMQDQRALLEKQKDNLRKEQSALKDSESQQKKSLDA
                     LTKQKALHQKAARELEESQKELQNKIRELMTQKRKLQESRAGITPTYVPSGGKLSWPV
                     KGEITSNFGTRVHPVFKTKIMHTGLDIKASAGTPVKAAEAGEVLFAGWLRGYGQIVIL
                     DHGGDLTTVYAHLSQIRVEEGKVVKGGDVIGNVGSTGVTSGPHLHFEVRVNGEARNPI
                     TFLGR"
     gene            5171..6373
                     /locus_tag="PK146_02570"
     CDS             5171..6373
                     /locus_tag="PK146_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPE91155.1"
                     /translation="MFRRARDIFIGAVIGVILVGGVMSAVAFNDVDLFKAAPFSFQDL
                     WIMKQARVILETYHVDGEEGISEENLLHGAIRGMVSALDDPYTRFVDPDELKEEEIEL
                     QGEYGGLGIYIGQRDGKTLVVSPIEDTPADRAGLKPKDQIVKIGDEVILGWSQNEVVN
                     TLRGDPGTKVVIWIRREEEEDLLKFEIERESIRIQTVKSELLEDSVGLVRITQFNQRT
                     LFDFRKAVYDLLGEGAGSFLIDLRNNPGGLLSSCVDIADLLLDGGVIVSTRGRFDRAN
                     EVIYARHGTITDLPVAVLINEGSASASEILAGALKDQGRAVILGKQSFGKGSVQTLFN
                     LSDSSGIFVTIAKYYTPAGTVIDKVGLSPDIEVEGEFERDREKDVQLKRALSELLGRE
                     DATEAKDS"
     gene            6348..7127
                     /locus_tag="PK146_02575"
     CDS             6348..7127
                     /locus_tag="PK146_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPE91156.1"
                     /translation="MRQRLKTASRVILVVLWVLAFPRLSECSGGFIRPPVVAVIVDDL
                     GFSMDAARRLSSVPLPLTWAIIPYQRFSRTTAEMAGEMGIPFIVHLPMEAKGFPGDPH
                     ALVRAGMAPEEVRASVRNALWSLPGATGLNNHQGSRATEDPRAMKGVMLELKAEGLLF
                     VDSRTSGGSVAYPVALDSGLPATLNRVFLDHYDEEIFLQEQFRKTFALARKNGGAVAI
                     CHARPGTMDFLPKLYEMAPEDIEFVTVPEYLAHKRIGGWEE"
     gene            7132..>7632
                     /locus_tag="PK146_02580"
     CDS             7132..>7632
                     /locus_tag="PK146_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPE91157.1"
                     /translation="MKGRVEIIVGAQWGDEGKGRVVDAIANNMDVVVRFQGGANAGHT
                     VIVEGEKYIFHLLPSGMLYSGKTCVVGNGVVVDPQQLLEELGDLQERGKDRARLVISG
                     AAHVVMPYHKVLDGMEERLRGSGKKIGTTRRGIGPCYMDKVNRCGIRVEDLLCEDALR
                     EKLSFNL"
BASE COUNT         1673 a         1954 c         2420 g         1585 t
ORIGIN      
        1 aatggggcct gaagggagag atcgcccctg atccggtcct ggcgatggta ccgggttttc
       61 aaagaatggg agacggcgac ctccttctgg tcaacatcag gccatggcac ggagggcttc
      121 ccgaaacgac agcccttgcc tcggtatcgg tccaggagag gtgccgtctg gaaatgatcg
      181 ctgtggccct ctccgacgag gacgagtccc ttatgagcga tctcaggtca cgtgggatgt
      241 tcagcccttc ccgtatcgtt cggatcggcg ggatagagga agcaaggtcc ctgtggcgga
      301 gggcgaaggt ggcagttgga atgaggcttc acttctgcct gctctcggca cttgcaggaa
      361 taccgtgtct ggccgtaccc tatgatccca aggtcaggtt cttcgccgag gattgggggc
      421 tgcctgtctg ggagggcaag ttgccctctt cctttcctgg gatcggtctg aacaggtcaa
      481 ggatagagga agcctcggaa cggctccagg aagtgttcag ccgggtcctg ggaagcctgc
      541 ttgcagggga gtgatccaat gaaagcctcc gtaagacgtg aactggaaaa tgtggctctg
      601 gaggtcagga aggatatcgt caggatgacc gggctgtcaa ggtccggcca ccttcgttcc
      661 tgcctctccg tagttgacat taatgtctat ctatactgga aggttctcag gataagacca
      721 gaagagccac actggccgga gagggacaga ttggtcttca gcaagaacca cgcatgtcct
      781 gccctttacg ctgttctcgc caggcgcggt ttcttcgaaa gggaagagct ttggaattac
      841 aggcgtcttg gcgcaatgct tcaggggagg cccgagcact tccggactcc gggggttgat
      901 gccccaggcg gttcaggggg gatgggtatc ggccttgcca acggtctttt gatgtcggcc
      961 agaatgggcc gtcttgaccg gagggtcttc tgtgtgatgg gtgatggaga gctccaggtg
     1021 ggggcggtgt gggaatcggc aatgcaggct gccaacctgg gatccggtgc gttgacgacc
     1081 tttgtcgata tgaacgggat acagacagga ggatcggtcg aaagcataaa gaggatcgag
     1141 cctgtcagga agaagttcga agcctttgga tggcgtgtga tggaagtgga cggtcatgac
     1201 ttccgtcaac tggaaaaggc tgtcgcctgg tccctttcgg gtcccgggga gagcccctct
     1261 gccgttctag cgaaaacggt cccgggcaag ggtgtcacta tcgccgaaaa ggggctgctg
     1321 ggtccaccgg cctgcccgtt ggaccgggac aggatggaac aggccctcag ggagctggag
     1381 tcctcttcag gaggaagggg aggggaggtt tagccatgag aagtaccctt tcaggtttcg
     1441 gagaagggct gctgggaaca ggccgcaaga ggaacgacgt tgtgcttctc catgcggata
     1501 tggtcgatgc cgaggtcata accccctttt cccttgagtt cccggagagg accatcaact
     1561 gcggggtctc tgaacaggac atggtgatga ccgccgcggg cctatccctc ggcgggaaga
     1621 tccctgtagc cgtttcctac tctgccttcc ttaccgcgag gtcttttgac cagatcagga
     1681 cctgtctggc cattcccggt cttcaggcgg tcctggtcgg tacgcactcc ggtctttcag
     1741 caggagagga gggtgccacg caccagatga accaggatgt tgctctgatg aggagcttgc
     1801 ctgacatgtc tgtgctggtt ccatcggact acgcctcatc ttcggccatg gccgggatcg
     1861 catgcgggca cccgggaccc gtctacctcc gactcggacg ggacgaggtc cctgacattg
     1921 ggatcctgga tgagagcgag atgcgcatcg gaggcggaag ggcccttgtc gacggtgacg
     1981 gcgtcaccat atgtgcctgt ggtatcatgg tccacgaagc gatgaaggcc gcggccatcc
     2041 tttcccagca gggcatcaac gccgaggtga tcgactgctt cagcctgaag cccctgccgt
     2101 cgcagctcat cctcgcttcg ttgagaagga cgggttgctg cgttgtcgcc gaggagcaca
     2161 acattatagg agggctgggc gaggccattg cggccctgtc ggcctctgag aaccccgttc
     2221 ctgtaaggtt tgtcgccgta tctgaccggt acggacagag cggcacccct tcagagcttc
     2281 aggagtacta cgggttgacc ttcagggaga tagtggggga gtcggcccag gtatggtccc
     2341 tgcgcaggag gtagcagatg gagatacggt tcgcgggtgt gaccaagcag ttcctccccg
     2401 atatcgtcgc ccttcaggac attttcctta ccatcgagaa gggggagttc gtctatcttg
     2461 tcggaccgac ggggtcgggc aagacgaccc ttctgcggct tgtcacccgt gaactggtgc
     2521 cgacgaaggg tcgtgtgacc gtaggcgacg tcaatattcg caagatagct tcccacaacc
     2581 tggcctacct ccgacgtaac ataggtgtgg tcttccagga ttttcggctg ctcccggacc
     2641 ttaccgtata cgagaatatc gccttcgtcc aggaggtgat aggcgtatca agccatgatg
     2701 ttcagaaaag gaccaacgat gtgctggacc gtctcggtct ctggaggagg aggagcctct
     2761 tccctgaaca gctctcgggg ggggaacagc agagagtggc catagggagg gctattgcga
     2821 actcgccttc gatacttcta gccgatgaac ctacgggcaa ccttgatata gatacggccg
     2881 acgagatcat gcaacttctg ctgtccatca acgcttcggg gaccaccctg atagtggcga
     2941 cccacaacca gtacattgtc gattcatacc gccagagggt catcgagctc cgcaaggggc
     3001 gtctggtaag ggacgagaag aagggcaggt acagggtgga tggggacttt taggtacgtg
     3061 atgagggata cctggagact actctccagg cattcaggtt tgagcctcct gacgatcata
     3121 acctccgtgg cggtcttctt tctcgtgggg gcgagcattc tctttgtcct taacacccga
     3181 tacctcgtcg aggctgtaga gggcgacctt actgtccagg cctacgtcag ggattcccaa
     3241 gaagccctgg aggtggttgc acgaaaggct tcctcctacg attcggtctc ctcggtcagg
     3301 gtggtgacct ccggggaggc ccttgaacgc ctcaaagcca ggctcggcaa actggccgaa
     3361 gcagtgaccc tccttgacga gaaccctcta ccccccagcg tggagataca ggtcaagaaa
     3421 gcgtccttcg ccacggtcat agcccgagag ctgatagcca tgcctgaggt ggaggaagtg
     3481 gtctatgctg ggactgttgc cgagaggctg cagaagatat cccatttcgt ggccaggctg
     3541 tcaatagtcg ttctggtggt ctccctcgct tcggcggcac tggtcctctt caacacgatc
     3601 cgtattggcg tgttcgcacg gaaagaagag ataagcaaga tgctcctcgt cggcgcttcc
     3661 cggacctttg tggtcttccc ctatgttctc cagggggtta tcctggggac agcgggagct
     3721 gcgctctcgg tcctgctgtt gtggttctcc tacgggatgg ccatcaccgc cctggagaga
     3781 tcccttccct tcctcagact tctgaccgac aggatcatcg ttctccgggt cggtgcggtc
     3841 ctggtgcttg ccggcgtatc ggtcggatgg atctgcagct ggcttgcggc atcccgtttt
     3901 gtcaggcaag cttcaaggtc gatctagaac gagtctggag tgttgagatc atgaaggtaa
     3961 aagggaactc ttccaccgta cggtcactcc tcctggtttt ttcactgctg gtccttttcc
     4021 catctcgggt ctcatgtgcc cctgatatcg attcgaggat caaggatgaa cagggacggc
     4081 tggaatcgat agagaggcag atatcctacc accagaaaca gatcagcgag gcccggagaa
     4141 aggagcaggg actgcttgac gagctttcga agatagacca gagcgtaacc ctcctgaggc
     4201 agaaggttgc cctcctcgat ctccagcgcg agaagacgga gatgaggata aaagagctga
     4261 cctcggagat agagaccacc gaagcacaac tttcaaggac caagggttac cttgccagga
     4321 ggttcgaggc tatctacaag tacggtggga tcgcagagtt caacctcctc ctttcggcgg
     4381 gcagcaccca cgaggctctt aacaacgcct atcttctggc caggatagcc cgtgaggatg
     4441 aacgaaggat agtgaccatg agcgagaaca aaaaaaagct cgaatctgcc atgaaggaga
     4501 tgcaggacca gagggccctg cttgagaagc agaaggataa cctgagaaag gaacagtcgg
     4561 cgctgaagga ctccgagtcc cagcaaaaga agtcccttga tgccttgacg aagcagaagg
     4621 ctcttcacca gaaggcagca agagaactgg aggaatccca gaaggagctt cagaacaaga
     4681 tacgggaatt gatgacccag aagagaaagc tccaggagtc gagggcaggg ataacaccca
     4741 cctatgttcc ctcggggggg aagctgtcct ggcctgtaaa gggagagata accagcaact
     4801 tcggtacaag ggtccacccc gtgttcaaga ccaagatcat gcataccggg cttgatatta
     4861 aggcttcggc cgggacaccg gtcaaggccg cggaggcggg agaggttctt tttgcaggtt
     4921 ggctgagagg ctacggccag atagttatcc tagaccacgg gggggatctt accaccgtat
     4981 atgcgcatct ttcccagatc agggtggagg agggaaaggt cgtcaagggc ggggatgtta
     5041 ttggaaatgt ggggagtact ggagtgacct ccgggcccca tctccatttc gaggtaaggg
     5101 tcaacggaga agcaaggaac ccgatcacct tcctcggaag gtagggtaga gggaagggtg
     5161 gaatcctgaa atgtttcggc gggcaaggga tattttcata ggtgctgtaa taggggtgat
     5221 cctggtaggc ggcgtaatgt ccgctgttgc cttcaatgat gtggatctct tcaaggcagc
     5281 ccctttcagc tttcaggacc tgtggatcat gaagcaggcc cgcgtcatac ttgagaccta
     5341 tcatgtcgac ggggaggagg ggatctccga ggagaacctt cttcacggag ccatccgggg
     5401 catggtctct gcgctggatg acccctacac ccgtttcgtt gaccctgatg aactgaagga
     5461 ggaagagata gaacttcagg gcgagtacgg aggtctcgga atttacatag gtcagcgaga
     5521 cgggaagacc ctggtggtga gtcccataga ggatactccc gcagacaggg ctggtctcaa
     5581 gccaaaggac cagatagtga agatcggaga cgaagtgatc ctcggatggt cccagaacga
     5641 agtggtcaac accctcaggg gggatcctgg cacaaaggtg gtcatttgga tccgtcgcga
     5701 ggaagaggag gacctgctaa agtttgagat agagagagag agcataagga tccagactgt
     5761 caaatcggaa cttctggagg acagcgtcgg gcttgtcagg ataacccagt tcaaccagag
     5821 aacccttttc gatttccgga aggcagtcta tgatctgctt ggagaagggg ctggaagttt
     5881 cctgatagac ctgaggaaca accccggggg actactcagc agctgtgtgg atatagcgga
     5941 cctgcttctc gatggaggag tcatcgtaag taccagggga cgtttcgaca gggccaacga
     6001 ggtcatatat gcaagacatg ggaccatcac ggacctcccc gtggcagttc tgatcaatga
     6061 aggcagtgcc agtgcctcgg agatccttgc cggagccctg aaggaccagg gaagggccgt
     6121 catcctgggc aagcaatcct tcggaaaagg atctgtccag acccttttca acctgtccga
     6181 ctcgtcgggc atctttgtca ccatagcgaa atactacact cctgcaggaa ctgtcatcga
     6241 caaggtgggt ctttctcccg acatcgaagt ggagggtgag ttcgaaaggg acagggagaa
     6301 ggacgtccag ctcaagagag cgttgtcgga gcttttgggc agagaagatg cgacagaggc
     6361 taaagacagc tagcagggtc attctggtcg ttctgtgggt cctggccttt ccgcggcttt
     6421 cggaatgctc gggggggttc atccgccccc cagtagttgc cgttatcgtg gatgacctgg
     6481 gtttctccat ggacgcggcc aggcgacttt cctcggttcc cctgcccctt acgtgggcga
     6541 tcatacccta ccagcgcttc agtcggacaa cggctgagat ggccggtgaa atgggtatcc
     6601 cgttcatcgt tcacctgccc atggaggcca agggtttccc cggtgacccg cacgctctgg
     6661 tacgggcggg aatggccccg gaggaagttc gggcctcggt gaggaacgca ctgtggtcgc
     6721 tgccgggggc cacaggcctg aacaaccacc agggatcccg ggcaacggag gatccccggg
     6781 cgatgaaggg ggtaatgctg gaactgaagg cggaggggct tcttttcgtt gacagcagga
     6841 cctccggagg ttccgtggcc tatccggtag ctttggattc cggtcttccc gctaccctga
     6901 acagggtctt cctcgatcat tacgacgaag agatttttct tcaagagcaa ttccgcaaaa
     6961 cctttgcgct ggcaaggaag aacggagggg ccgttgcaat atgccacgcc aggcccggga
     7021 ccatggattt tcttccgaag ctgtacgaaa tggcccctga ggatatagaa tttgtgactg
     7081 tgcccgagta cctggcgcat aaaaggatcg gcggctggga ggaatgaccg catgaagggt
     7141 cgggttgaga taatagtcgg cgcccagtgg ggtgacgagg gaaaaggaag agttgtcgat
     7201 gcaatagcca acaacatgga cgtggtcgtc aggttccagg gcggagccaa tgctgggcac
     7261 acggtaattg tggaaggcga aaagtatatt tttcacctcc tgccctcggg gatgctctat
     7321 tcggggaaga cctgtgtcgt cggcaacggg gtggtcgtag atccccaaca gctcctggaa
     7381 gagctcggag acctgcagga gaggggcaag gacagggcgc gtctggtgat cagcggagcg
     7441 gctcacgttg tcatgcccta ccacaaggta ctggatggaa tggaagagcg tcttagaggg
     7501 agcggaaaga agatcgggac aacgcgcagg gggataggcc catgttacat ggacaaggtc
     7561 aaccgctgcg gcatcagggt cgaggatctc ctttgcgagg acgcgctgag ggaaaagctc
     7621 tccttcaacc tg
//
LOCUS       DAOPKQ010000044         7597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10326_length_7597_cov_5.431583, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000044 DAOPKQ010000000
VERSION     DAOPKQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10326_length_7597_cov_5.431583"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK146_02585"
     CDS             complement(<1..534)
                     /locus_tag="PK146_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE91158.1"
                     /translation="MKFDMSPDYVILFERLFDNSPEGVVIQGLDGVVYKANRAFCEMF
                     GYSLEEVIGKDLDSLVAADKNLRGEASRRTKSATMGEQFMFESIRQKKDGTKFHVLAF
                     GVPITSEGQLVAIYGMYRDISDRKMAEEALQRSEERYRAIVQQQQEVIVRYSPDWSVT
                     FANDAYCRYYQTTPEEIL"
     gene            737..910
                     /locus_tag="PK146_02590"
     CDS             737..910
                     /locus_tag="PK146_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91159.1"
                     /translation="MSKEKVIITNLACTSDLCSMVEFDAQLLRDLIDDLESGWVETRT
                     QDPKCDKDCKYCR"
     gene            complement(992..1393)
                     /locus_tag="PK146_02595"
     CDS             complement(992..1393)
                     /locus_tag="PK146_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91160.1"
                     /translation="MLYFQFLSLVFGTVMVSLAPAIVIRGERWVDLFSELFFPEKQPV
                     WLWVAGAASAFLVLITWYVELTSSVRLSWVMTLFITLSLVKSYFLIFRYEPSRRTIMD
                     MMGKGRSFTIGLAGIMYLAGFCILCLGIFAF"
     gene            complement(1412..1819)
                     /locus_tag="PK146_02600"
     CDS             complement(1412..1819)
                     /locus_tag="PK146_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91161.1"
                     /translation="MKKKTDRKSTVKKAILLSLLFLAAIVTTYVIFVTRIDPKIEAIE
                     LVKREYLPKERLQEFVSDINRQGKFKLEGWKIEETGEKGVFLVSYSIRWLDDGGFRTG
                     DPVGFWFRVSPERGFCDPIQPGSTVTPQMTEGQ"
     gene            1989..5267
                     /locus_tag="PK146_02605"
     CDS             1989..5267
                     /locus_tag="PK146_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91162.1"
                     /translation="MSLVAVFPAGAQEDLCWLEPGEPVPEQFAFSGEPPALEEVNEAF
                     LKAFGGEPSLWPEELRNIHEVQVAFNKRALASSKFKGQFSQEALAKMRAQEKDPVGYW
                     SRCLHEGWGFEEWFNHDHLTVQRVPRALLDRFMTEVFPLTWRDIRSIVHVQEYMLELS
                     DEQFIPYNDYDSRVLDLRKRDVQEYNSLVEDAAEGYIKGNDFLMLLLMPERIDTNWVG
                     DEAFEASLVKQAKRLSEKVQDVVSDMDRRFRSAFYRAFPKLTYSWNAEPWSEDHVVFN
                     GKPVQRGPSIDLLGTSTRIEHPYIGEKITIQEQKVTSGPTLKLQWGTVPSHSSEFDKG
                     AVVEKVSPRYMVARKQYGESREFILSLYLDHLSGFGHEVSFGPAGASPFPEEIGAEIM
                     EAITEAFLEAAEVLYSGPVVMTAKDLSSAEGLILQPGEQKGEANKARKVTLDPPTVND
                     MHVDHDLPEGWSFSVVVWEDGRPVPQARVAIRKPEAGVLSSRTAEGGDEKWLLLRTGP
                     LGEAVINYTPPSLTELKMMGPSQWQVNIVAEDQESGAKASVTFRITRPLGLDVAVEHP
                     VLPADPGFQNTLRLRFDGRNPQGDRDEKFRVKITVKSGNGALSQDPEGYGGKSAFAME
                     VESDVDHVLYYRWFGPRDLDGPVTETVLFQVPELELEGTASFLVGVAPSIHSTQQEQL
                     GVEQPGLFVPLKVYVQDEYHPDLDMAEFFRSFQLKPVLDISQVGFKPIDPDDSLSRQI
                     LEALLGHLKGAELPRDAVPLEPETWSLARDAGSRWFLVGGVTPAGSAPRNAFPGIILW
                     DYGDYTFRISMNLEDAHGLPVEPLKRTMTKILHVRPFPTGDKRAELILPMILAYSAMF
                     PGEEARQFSVRSRNLLQRGDLATASVTIGEYFSKRLSSVSPDSDIDLHDMERLEYLVH
                     KAHGVSGAPLSETILEGSLSQAKLNFLCAAAGEYAEIFLSQGYDLSKLVDPPSLDRSS
                     PELEILEMIKGFLEGYGGYGILALARENIKSLEIYSESGEELSEFHGQVFGGGGGPRR
                     VFFGKNSVVVPFLLGENLLINLRGKGKPVDAIKILPNGINVQRYGFHPGSETINVYGD
                     VVRP"
     gene            complement(5410..6420)
                     /locus_tag="PK146_02610"
     CDS             complement(5410..6420)
                     /locus_tag="PK146_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HPE91163.1"
                     /translation="MEVIRATSVVDTHTGGEPTRIIIGGGPYLPGRTISEKWAYMKEH
                     MTDFRNFVMHEPRGHSDMFGAFLTPPEKESSHYGVIFMDSGDGVSMCGHGSIGTAHTL
                     VELGMVPREEPHTEVVLDTPAGQIRLKVAVKDGKVGDVTLANVPAFVFRQDVELPVPS
                     LGGKKVHMDIAFGGNFFAIIHAEELGLTLEREEVPRLLSLGLEILREANRTVKVQHPT
                     EKHLNYIGLTEFALERPGKPTKNCVIFGESNVDRSPCGTGTCAKMALLAARGLLKPGE
                     VFDHESVVGSVFHGNYEPGPKIGEFETILPFIRGRASITGFNFLLQQKNDEMGSGFLL
                     PK"
     gene            6623..>7597
                     /locus_tag="PK146_02615"
     CDS             6623..>7597
                     /locus_tag="PK146_02615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPE91164.1"
                     /translation="MGRNTADVAIIGGGVHGCSIAYHLAKRGVKTVLFEKDYISSGSS
                     GRSAAGLRYQFGTEVNCRLAKYNMEKIPHLQEELEYKADLEYDPAGYLWIAYSETQLE
                     QLRKNVALQNSLGVDSVILDPVGIKEVAPGLNVEGMLGASFNQKDGHVNPHTMNFAYA
                     DAARRFGAEIHTKTTVTGLLTDNKKVWGVKTTKGDWEVGQVVCAANAWAAEVGKWCGL
                     EIPIEPERHSLLITEPVERMKHPMTLCLDDGSYWKQCPNGTFMLGWGNKDEKKDTDYY
                     SSWQFLEEVTQRVLAKMPCLAGVRCVRQWTGPYGITPDEQAIMGETPVE"
BASE COUNT         1722 a         2092 c         2132 g         1651 t
ORIGIN      
        1 gagtatctct tcgggcgttg tctggtagta tctgcagtag gcgtcgttgg cgaaggttac
       61 ggaccagtcg ggagagtagc gcacgataac ttcctgctgt tgctggacaa tggcgcggta
      121 gcgctcctcg cttctttgaa gcgcttcctc tgccatcttg cggtcggaga tatcccggta
      181 cataccgtat atagccacca gttggccttc ggatgtaatg ggcacgccga aggccaggac
      241 gtggaacttg gtgccgtcct ttttctgcct tatcgattcg aacatgaact gttcgcccat
      301 ggtggcgctc ttggtccgcc tggaggcctc gcccctcagg ttcttgtcgg cggccaccag
      361 gctgtcgagg tctttcccga taacttcttc aagggagtat ccgaacatct cgcagaaagc
      421 cctgttagcc ttgtaaacaa caccatcaag cccctggatc accactcctt ccggggaatt
      481 atcgaaaagt cgctcgaaaa gtatgacgta gtcggggctc atgtcaaact tcattgaact
      541 gttcctcctc ggaaagaggg gggtatattc acacgggaaa cagtataaca tgaagtacac
      601 ttttaaccca atatcccctt gaaggtcttt tctccggaag ggctgtaccg ggtcttgtta
      661 cggtctggtc tggggtgtta tcatcttacc ggcagatgga caaggaaacg acccttaatg
      721 cccgggagga tccaaaatga gcaaagagaa ggtcattata acaaacctcg cctgtacctc
      781 agatctgtgc tccatggtag aattcgacgc ccagctccta agggatctta tcgacgatct
      841 tgagtcgggg tgggtcgaga cgcggaccca ggaccccaag tgcgacaagg actgcaaata
      901 ctgcaggtga aaggctgctc tacctggata aaaatgggga agccggcccc gttgggggtc
      961 ggcttttttg tgccgttccg ggacgttatt gtcagaaggc gaatatgcca aggcagagaa
     1021 tgcagaaccc tgcaaggtac ataatccccg ccaaacctat cgtaaaggat cggcccttcc
     1081 ccatcatgtc catgatcgtt ctcctggacg gttcataccg gaagataagg aagtatgatt
     1141 tcaccagcga aagggtaatg aagagggtca tcacccatga cagtctcact gatgaagtga
     1201 gctccacgta ccaggttatc agaaccagga atgccgaagc cgccccggcg acccatagcc
     1261 agacgggctg tttttcgggg aagaataact cgctgaacag gtctacccat ctctcacccc
     1321 ttatgactat tgctggtgcc agggaaacca tcactgtccc gaaaacaagt gaaaggaact
     1381 ggaaatagag catgctctct cctctccggg gttactgccc ctccgtcatt tgcggggtta
     1441 cggtggatcc gggctggata gggtcacaga accccctctc cggggatact ctgaaccaga
     1501 aacctacggg atctccagtc cggaaacctc cgtcgtcaag ccagcgaatg gagtagctga
     1561 caaggaagac ccccttctca ccggtctcct cgatcttcca accctccagt ttgaacttgc
     1621 cctgtcgatt gatatcagag acgaactcct gcagacgttc cttcggcagg tactctcttt
     1681 tgacgagttc aatggcctct atctttggat cgatccttgt gacgaagatg acataagtgg
     1741 taactattgc ggccaaaaaa agcagggaaa gcagtatcgc tttcttcaca gtgctctttc
     1801 tgtctgtttt tttcttcatt cctgcaggcc acctctctag ataacattat acataagacg
     1861 ccccattttt aacggggata cccccctgac cttcctcctg ccggtaggtt agaatatgtt
     1921 ccggggagtt ggtatttttg aaccctaaaa taaaggaact gtgcctttcc cttttcttgc
     1981 ttttttccgt gtccctcgta gccgtattcc cggccggcgc gcaggaagat ctatgctggc
     2041 tggagcctgg ggagcctgtc ccggagcagt tcgccttcag cggagagccc cctgctctcg
     2101 aagaggtcaa tgaagctttc ctgaaagcct tcgggggaga accgtccctc tggccggagg
     2161 agttaaggaa cattcacgag gtccaggtgg ccttcaacaa gagggctctt gccagctcaa
     2221 aattcaaggg acagttcagc caggaggccc ttgcgaagat gagggcccag gaaaaggacc
     2281 ccgtcgggta ctggtccaga tgcctgcacg aaggctgggg tttcgaggag tggttcaatc
     2341 acgaccacct gacggtgcag cgggtcccca gggccctgct tgaccgcttc atgaccgagg
     2401 tcttccccct gacttggagg gatatcagga gtattgtcca cgtccaggag tatatgctgg
     2461 agctatccga cgaacagttc atcccctata acgactacga cagcagggtg cttgacctga
     2521 gaaagcgaga cgtccaggag tacaactccc tggtggagga tgcggccgaa gggtatatca
     2581 agggcaacga cttccttatg ctgctcctga tgcccgagag gatcgatacc aactgggtgg
     2641 gtgacgaagc cttcgaagcc tccctcgtga aacaggcaaa aagactctcc gaaaaagtcc
     2701 aggacgtggt cagcgacatg gacaggaggt tccgttcggc tttctacagg gccttcccta
     2761 aacttaccta ttcctggaac gccgagccct ggtcggagga tcacgtagta ttcaacggga
     2821 aacctgtcca gaggggacct agcatcgacc ttctgggaac gagcaccagg atagagcacc
     2881 cctatattgg cgagaagatc accatccagg agcaaaaggt gacctccggc ccgacactga
     2941 agcttcagtg ggggacggtc ccgtcccact cgtcagagtt cgacaagggg gccgtagtgg
     3001 agaaggtctc tccaaggtac atggtggcca ggaagcagta cggggagtcc agagagttca
     3061 tcctttcact gtacctggac catctctccg gtttcggcca tgaggtatcc ttcggtcctg
     3121 ccggggcatc ccccttcccc gaagagatcg gcgcggagat catggaagct attaccgagg
     3181 cctttcttga ggcagcggag gtgctataca gcggtccggt ggtcatgacc gccaaggatc
     3241 tttcttccgc cgaggggttg atcctccagc ccggtgagca gaaaggtgag gcaaataaag
     3301 caaggaaagt aacactcgac ccgcccacgg tgaatgacat gcacgtcgat cacgatctcc
     3361 ccgaagggtg gtccttttca gtggttgtat gggaggacgg tcgtccggtc ccccaggcca
     3421 gggtggccat aagaaagccc gaggcagggg tactctcttc caggaccgca gaaggtgggg
     3481 atgagaagtg gctcctcctg agaacggggc ccctgggaga ggcagtgatc aactacactc
     3541 caccctccct gacggaactc aagatgatgg ggccaagcca gtggcaggtc aacatcgtgg
     3601 ccgaagacca ggagagcggc gccaaggctt cggtgacgtt caggatcacc aggcccctgg
     3661 ggctggatgt tgctgtggaa caccctgtcc tcccggcaga tccgggattc cagaatactc
     3721 tccgcctacg ctttgacgga cggaatcctc aaggtgacag ggacgagaag ttcagggtca
     3781 agataacagt aaagagcggg aacggagctc tctcccagga ccccgagggg tacggcggca
     3841 agagtgcttt tgccatggaa gtggagtccg acgtggatca cgtgctctat taccggtggt
     3901 ttggcccaag ggatctcgac ggtcccgtga cggagaccgt cctcttccag gtccccgaat
     3961 tggagctgga ggggacagcc tcatttctgg tgggggtggc ccccagtatc cattcaactc
     4021 aacaggagca actgggcgtg gaacagcccg gactgtttgt tccactgaag gtctacgtcc
     4081 aggatgagta ccaccccgat ctggacatgg ccgaattctt ccgctccttc cagctcaagc
     4141 cggtcctgga catcagccag gtgggcttca agcccatcga tcctgacgac tccctctcaa
     4201 gacagatact ggaggcactt ctgggtcacc tgaagggagc cgaattgccc cgcgatgctg
     4261 tccccctgga accggagaca tggtcccttg ccagggatgc cggttcccga tggtttctcg
     4321 tcgggggcgt aacacccgct ggtagcgcac cgaggaacgc cttcccgggg atcattctct
     4381 gggactacgg tgactacact ttcagaataa gcatgaacct cgaggatgcc catgggctcc
     4441 ccgttgaacc tcttaaacgc accatgacca agatcctcca tgttcgtccc ttcccgactg
     4501 gagacaagag agccgaactt atcctcccca tgatccttgc ctattcggcc atgttcccag
     4561 gtgaggaggc gagacagttc tccgtacgca gcaggaatct ccttcagaga ggcgacctgg
     4621 cgaccgcgtc ggtgaccata ggggagtact tttcaaaacg tctttcgtcg gtctcccctg
     4681 acagtgacat tgaccttcat gatatggagc gacttgaata cctggtccac aaggcacacg
     4741 gagtgtcggg cgcccctctt tccgaaacga tcctggaggg atccctctcc caggccaagc
     4801 tgaactttct ctgcgccgca gccggggagt acgctgaaat attcctctct cagggttacg
     4861 acctttcaaa gctggtggat cccccttctc tggaccgaag cagccccgaa ctcgagatcc
     4921 tggagatgat aaaagggttc ctcgaggggt atggcggata cgggatcctt gctcttgcca
     4981 gggagaacat taagtccctc gaaatataca gtgaatcggg ggaagagctg tccgagttcc
     5041 acggccaggt ctttggcgga ggggggggtc ccagaagggt tttcttcgga aagaactccg
     5101 tcgtggtgcc cttccttctc ggcgagaacc tgctgatcaa cctgagaggc aaggggaagc
     5161 ctgtggatgc aataaagata ctgcccaacg ggatcaacgt acagcgctac ggcttccacc
     5221 ccgggagcga gactatcaat gtctacgggg atgtggtgag gccctgaaaa accgatatag
     5281 aaatagcgta aacggtaaac agtaaacgga atggcaaaaa atcaaaagac cctgttctgc
     5341 tcttgacttt accgttcacc gttgaccgtt cacgcctttt gatcttttga gtcacgactt
     5401 acgttccttc tacttcggca acaaaaaccc cgatcccatt tcgtcgttct tctgctggag
     5461 aaggaagttg aaccccgtga tgctcgccct cccccttatg aatggaagga tcgtttcgaa
     5521 ctctccgatc tttggacccg gttcatagtt accgtggaag acgcttccca cgacgctctc
     5581 gtggtcgaaa acctccccgg gcttgaggag ccccctggcc gccaggaggg ccatcttggc
     5641 gcaggtcccg gttccgcagg gggagcgatc cacgttgctc tcgccgaaga tcacgcagtt
     5701 cttcgttggc ttgccgggcc gttccagggc gaactccgtc agaccaatgt agttcaggtg
     5761 cttctcagtg gggtgctgca cctttacagt gcggttggct tcacggagta tctccagtcc
     5821 aagggatagc agccgcggga cctcctccct ttccagggtc agccccagtt cttcggcatg
     5881 gattatggca aagaaattcc cgccaaaggc gatatccatg tggaccttct tgccgcccag
     5941 agatgggaca ggcagctcca catcctgcct gaagacgaag gccgggacgt ttgccagcgt
     6001 cacgtcgccg accttcccgt cctttaccgc caccttcagc cttatctgcc ctgcaggggt
     6061 atcgagtacc acttcggtgt ggggttcttc cctcgggacc atgcccagct ccaccagagt
     6121 gtgggccgtc cctatggagc cgtgaccgca catggagaca ccatcgccgg agtccatgaa
     6181 gatgaccccg tagtgagagc tctccttttc ggggggtgtg agaaaggctc cgaacatgtc
     6241 actgtgtccg cggggttcgt gcattacgaa gttgcggaaa tcagtcatat gttccttcat
     6301 gtaggcccat ttttcgctga ttgtccttcc tggcagatag ggcccccctc cgataatgat
     6361 ccttgtcggt tcgcccccgg tatgggtgtc aacaactgaa gttgccctga tcacttccat
     6421 cgggtacatc tcctttcggt cactccggtt atgtgaaaag gagtttcctg tccttacagc
     6481 atcgaaccaa aagtgcaata aagcgtttga cgcaaatggg ctaagaaaat aaaatctagt
     6541 atcgggttat taagattata tgcatgggtt cagaagcagg caaattttgg tcttcattct
     6601 cccatggaag gggtcgggga aattgggtcg caatactgct gatgtggcga taattggagg
     6661 cggggtgcac ggctgttcca tagcctatca tctggccaag agaggagtaa agacggtcct
     6721 gttcgagaag gactacatct cctccggttc gagcggcaga agcgccgcgg gtctcaggta
     6781 ccagttcggg acggaggtca actgcaggct ggccaagtac aacatggaga agatccctca
     6841 tctgcaggag gaacttgaat acaaggccga cctcgaatac gacccggccg gctacctctg
     6901 gatcgcctac agcgagaccc agctcgagca actcaggaag aacgttgccc tgcagaactc
     6961 gctgggcgtc gattctgtta tcctcgaccc tgtggggata aaggaagtcg ctccggggct
     7021 gaacgtggag ggtatgctcg gggcaagctt caaccagaag gacggacacg tgaaccctca
     7081 cacaatgaac ttcgcatacg ccgacgcggc aaggcgtttt ggagcggaga tccatacgaa
     7141 aactaccgtc accggtctgc tcacggacaa caagaaggtc tggggcgtga agacgaccaa
     7201 aggcgactgg gaagtcggcc aggttgtctg tgctgccaac gcatgggcag ccgaagtggg
     7261 caagtggtgc ggtctcgaaa taccgatcga accggaacgg cattcacttc tcatcactga
     7321 gccggtcgaa aggatgaaac acccgatgac cctctgcctc gatgacggca gctattggaa
     7381 gcagtgtccc aacggtacat tcatgcttgg atggggcaac aaggacgaga agaaggatac
     7441 agactactac tcttcatggc agttcctgga agaagtaacc cagagggtgc ttgcaaagat
     7501 gccctgtctt gctggtgtaa ggtgtgtacg ccagtggacg ggcccctatg gaattactcc
     7561 tgacgaacag gccatcatgg gtgaaacgcc ggtggag
//
LOCUS       DAOPKQ010000045         7564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10385_length_7564_cov_5.501531, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000045 DAOPKQ010000000
VERSION     DAOPKQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10385_length_7564_cov_5.501531"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK146_02620"
     CDS             <1..573
                     /locus_tag="PK146_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HPE91165.1"
                     /translation="WDNWILSNTLTYRSTDHDTFRAQVGGDARASFDSWAITNDIRLG
                     YVMKEIGEDSNWQIIPEVGLNVGHINRGGYTEANGFTYGDYSNTVVESVVGIRFKAEY
                     LRDDGSTFVPQLRLAWIHILSGEDITIDQTWGSDTKWFTETLDRDYFVADIGMSLYKF
                     NNMDISLNYSGRFSDNSTSHGGWIRLQWEF"
     gene            complement(787..1113)
                     /locus_tag="PK146_02625"
     CDS             complement(787..1113)
                     /locus_tag="PK146_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91166.1"
                     /translation="MATVLPSILFFTRFPSFFKAGRVWIFRAFYNGGPASVNRPSVIK
                     PDYMRQASGIRKSENPGSGKIKGVNGTGKNQKQGNYPQMCADESKARLWCKIVLGTCS
                     FVSPIV"
     gene            1656..>1865
                     /locus_tag="PK146_02630"
     CDS             1656..>1865
                     /locus_tag="PK146_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91167.1"
                     /translation="MPVRIAGYYRVGEIEAELAQLGRERDPSPLFLVPSPGDRELLRD
                     ILVESVSFGSSEPPVLRWEDLYREAS"
     assembly_gap    1866..1875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1876..4592
                     /locus_tag="PK146_02635"
     CDS             <1876..4592
                     /locus_tag="PK146_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPE91168.1"
                     /translation="MELPREKRKRQIDPPDHWLIVRRVLKCFMARVDEQSIPPGVRHG
                     GFVWTLGEDLRELLREEVDPEALALSLGCISCTEGPGCTRLPAPGALLCRLYRDYLSY
                     LEHHSLTDSAQSASITRNILSGNPARAASWVAGRAFVFAGFMSFTRSQTGLIRQLSSL
                     GARVIVFMPESGLDIHDARAQLTTNLQSANSRTIPEPVPLLPVSAGDHRMELETLARN
                     LSLWSAGGGEFHMRLEASFPGWGNIGLYVDPSRLGCAEEILARYRIPYTVNGGPRVSE
                     TPLWKTASSAIEAGSLGYPAEETAHILSQPWISPAGFPLSRCLNEGPRGEDAWRSFLG
                     EHGDSGISAFFERMASFVSFIARGGTPSELLRSLRSLSGMGDPPDTGLSISRFIIDHP
                     SLDETARRLNAAVRELDQKLERMAEMETGIGPAGVDTLKGGDAVAFLSAWSERSTVWQ
                     PPGKRNSLHLYAGSPPVLARHRIFIATGLTADAWPGRLRESPLLNDSHKEAIHNNPDA
                     GLSPSHLPLLREKRVQREALLMRTIATADELCIASHPSQDASGRPLQPSRFLDSAISR
                     TPAWAVHAGGEPPFSRSMRDVLPRDDETVIEKVEARSSDPPRVPARIKMLPPATPWPS
                     GMVKKVPVSGIDTWKACPFKFYASRVLKIEKGRPYGFDPLEAGNLVHAVWEKVWHERM
                     DTGGNLTGLLEDCWDQAVEERYPSLKEFPRHMSRLKKNTWRMASLQQEMEDAGLAEAR
                     VSQEREQMIETDLNGVLFRGKFDRIDLLKDGSAIIFDYKTGRGNGLAGSMQLPAYAVL
                     LEKARNIKTSGYAFLSQADCSVTGRLEGPAGVILARWKKRSRSGLADMVSLASETLGA
                     MSVSVTESRFPPDYTNTEACRYCEFQEICRRKESPVTPPAEEEKGDAD"
     gene            4582..>7564
                     /locus_tag="PK146_02640"
     CDS             4582..>7564
                     /locus_tag="PK146_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPE91169.1"
                     /translation="MPTDIFRRYLEETACLEPRQIKAVMADDPLVVVSAGAGTGKTLT
                     LAWRFVRLVAANQIPVDRILTITFTEKAALEMRERIRRLMEEVRDGIPELSHLMDDPL
                     SRLEDAYISTIHAFSMRVLTECGLSADVDPGIRVITPPEENAFWQYLERSIDRETTQI
                     LAETLGGEWRERADEIFSAEGTADLVNAFGAGAICDAASSAIPLFESRGLDPEALWNW
                     ADELPERDSELSTGLLEVYTPEWQNTWETWMKMVLPEAGGPDIFRKDSTKFSARASDF
                     MESWENRPEKSELPRFVMALLDKEGLLGNLAGATGKCMKAVDAASLSVTGESLKDFRN
                     SRIPWVPAAQWIMKGFTGAETRLRSHLLKIIALCWRLFVSAKAARGTLSFDDMIRGAR
                     DAVSAEPSFAGKFLHVIVDEFQDTNSIQDELLSALTPPGGTLFLVGDLQQSIYRFRHA
                     EPAIFWRRIREALTQNPDSLISLDVTFRCRQSVMDRVNSLFGHTWREGIARSIKMAFS
                     PLAPPGSRDWWTKRQETTVSPFELVIPDTRDLEEELNTAGIRVAALRILSDRILEAID
                     KGATVWDSDGAGGFDARPVSFRDFAVLVPTRAFYDQIEQVFIEERGIPTYFEGNRNYF
                     GRGEVRDAVNLLEAAADPDDTLAMSSFLASPLSGLSLEVSAELISRSVSESVSLRKLF
                     SEECPDDAARFEDLRRTGLAAGPSLALAKLLDNISVILSCPAWKRPRLAANLRRAVDM
                     AREYEASMGMSLSGCASYLRYMTVKGIDSKEADTLGDQDDMVRVMTVHSAKGLEFPVV
                     AVAGLEQGLRTRSQGIRLIPSPHLGIAATVFPDGWSPDRDEKTLAGTIHDILETLEAR
                     EEKQRLLYVATTRARDSLILLGACPIEGGIPRARKHSWLETVRDWMGIAGGLSLSPVA
                     GSGVSVPIASSIPATTYRGGTVRLPERTGETLERISATAYALIRYCPNAFRMRHRQGL
                     GISWELNSEGAGGADLGSLAHW"
BASE COUNT         1731 a         2179 c         2229 g         1415 t
ORIGIN      
        1 tgggataact ggatcctctc caacaccctg acctaccgct ccaccgacca cgacaccttc
       61 agggcacagg tcggcgggga tgccagggcc agcttcgaca gctgggcgat caccaacgac
      121 atccgccttg gctacgtcat gaaggaaata ggagaagact ccaactggca gatcatcccc
      181 gaagttggcc tgaacgtggg gcacatcaac aggggaggtt acacagaggc caacggcttc
      241 acctatggag actacagcaa caccgtggtg gagagcgtgg tcggtatccg cttcaaggcc
      301 gagtacctga gggacgacgg ctccaccttc gtacctcagc tgcgcctggc ctggatccac
      361 atcctctctg gcgaggacat caccatcgac cagacctggg gaagcgacac caaatggttc
      421 accgagaccc tggacaggga ctacttcgtg gccgacatcg ggatgtcgct gtacaagttc
      481 aacaacatgg acatctcgct caactacagc ggccgcttca gcgacaacag cacatcccac
      541 ggcggctgga taaggctgca gtgggagttc taggaaatag caaggcggca ggaaacaggc
      601 tgcaggcaac aggaaaacct ggaacaaaac gagcgtgaga cacaagacgt tagacgtgag
      661 acgaaacaac ggaagcagga ccagcaatta ccgggggggc ggcgaatgcc gccccttttc
      721 cttcaaaacc aaaccttggg gtcagatctt gaattgcagc aattgcatac gctaatgggc
      781 gagaagtcag acgataggtg agacgaaaga gcaggtccca agaacaatct tgcaccacag
      841 acgggctttg ctttcatcag cgcacatctg cggataattg ccctgttttt ggttttttcc
      901 tgtcccgttt acacctttga tctttccgct tcccgggttc tcgctcttcc tgatgcctga
      961 agcttgtctc atgtagtctg gttttattac actcggcctg ttgacagatg caggcccgcc
     1021 gttatagaat gctcgaaata tccagacacg gccagctttg aagaaggagg gaaaccgcgt
     1081 gaagaacagg atgctcggca gcacagtagc catcggcgtc atcgtaataa ttattctcgt
     1141 tcttacgtgg gggtcccctc ctggggcggc cgtcgcgcta taggcgcgca accgcaaaca
     1201 ggaaccgggg cccccaggta caagggggcc ccggtttttt tatgtgagcg ggaccggatc
     1261 tggcaaaatc aggaagggga gagacaagat gcagaagagc agagacgttt tcacaaacca
     1321 gaccacagga aagggaaaca ctatgaccca gggaacaaca agtcatccga caggcaactg
     1381 gcataagcag ggtaaccagt cccaggaact ttacagcccc aggtggagag gacccttcga
     1441 ggggtataga taaatcaagg catcaggcta caggaaacag gcgtcaaaaa agaacaggac
     1501 ctggaaagcg taaacggtca acggtaaagg taaggcgaaa agataactgc tccaggtctt
     1561 gcagtgcacc gttcacgatt tacctttaac gtctgacgtt tcacgtttca cgcaaagcca
     1621 tccctcctgc taatataagg gcgggaggga cgccgatgcc tgtcaggatt gccggttatt
     1681 acagggtggg ggagatagag gccgaactgg cccaactggg aagagagcgt gacccgtcgc
     1741 ctctcttcct ggtaccgtcc ccgggcgacc gcgaacttct acgggacatc ctggttgaaa
     1801 gtgtttcctt cggatcatcg gaaccgcccg ttttgaggtg ggaggacctc taccgggaag
     1861 cctccnnnnn nnnnnagatg gaactacccc gggaaaagag aaagagacag atagaccccc
     1921 cggatcactg gcttatcgta agacgcgtcc tcaaatgctt catggcccgc gtggacgaac
     1981 agtccatacc tcccggtgtc aggcacggcg gatttgtctg gactctcggg gaggacctca
     2041 gggaactcct cagggaggaa gttgaccctg aagcgctggc tctttccctg ggctgcattt
     2101 catgcacgga aggtcccgga tgcaccagac tccccgctcc gggagcactt ctctgccgcc
     2161 tctacagaga ctatctttcc tacctggagc accactccct caccgacagc gcccaatccg
     2221 catcaattac acggaatatc ctttccggca accccgcccg tgccgctagc tgggtggcgg
     2281 gacgggcctt cgtatttgcc ggtttcatga gctttacccg ttcccagaca ggccttatcc
     2341 ggcaactctc aagccttggg gccagggtga tcgttttcat gcccgagagc gggttggata
     2401 tacatgatgc cagggcacaa ctgacaacca atctccagag cgccaattcc aggacgatcc
     2461 cggaaccggt accccttctc ccggtctcgg caggggacca tcgcatggaa ctggagaccc
     2521 ttgcaaggaa cctgtcactc tggagcgccg ggggtggaga gttccacatg cggcttgagg
     2581 catcttttcc tggctggggc aacatagggc tctacgtcga tccttcgcgc cttggatgcg
     2641 cagaggagat acttgccagg tacaggatcc cctataccgt gaatgggggt cccagggttt
     2701 cggaaacacc cctctggaag acggcctctt cggcgataga ggctggatcc cttggttacc
     2761 ccgcggagga gacggcacat atcctctcac aaccctggat ctcgcccgcc gggttcccgc
     2821 tgtcaaggtg cctcaatgaa ggccccaggg gggaggatgc ctggagatcc tttcttgggg
     2881 aacacgggga ctccgggata tcggcctttt tcgagcgtat ggcctccttc gtatcattca
     2941 tagccagggg cggcacacca tcggaactac tgaggtctct caggtcgctt tcagggatgg
     3001 gagatccacc ggatacaggt ctttccatat cacgtttcat tatcgaccac ccctcactgg
     3061 atgaaacggc caggcgcctc aacgcagctg tgagggaact ggaccagaaa ctcgagagaa
     3121 tggccgaaat ggagactggc atagggccag ccggcgttga cacccttaag gggggcgacg
     3181 ccgtggcctt tctctccgcc tggagcgaga ggtcgacggt ctggcagccg ccgggaaagc
     3241 gaaacagcct ccacctgtac gccggaagcc ccccggtgct ggccaggcac aggatattca
     3301 tagccacggg tctgactgct gacgcctggc cggggcggct tagggaatcc cccctgctga
     3361 acgacagtca caaggaggcc atccacaaca acccggatgc ggggctgtct ccgtcccacc
     3421 tcccccttct cagggaaaag agggttcagc gcgaggccct gttgatgaga acgatagcca
     3481 cggcagacga gctttgcatc gcttcccacc cctcacagga cgcttccgga agaccgctgc
     3541 agccctcccg tttcctggat tcggccatct caaggactcc tgcgtgggcg gtacatgccg
     3601 gcggcgagcc tccgttctcc aggtcgatga gggacgtgtt gccgagggac gacgaaacag
     3661 tcatcgaaaa ggtggaagca agaagttccg acccgccgcg ggtaccggca aggataaaaa
     3721 tgcttccccc tgcgacaccc tggccctccg ggatggtgaa gaaggttccg gtaagcggga
     3781 tcgacacctg gaaagcttgc cctttcaaat tctacgcttc gagggtcctg aagatagaaa
     3841 agggccgccc ttacggattc gacccccttg aagcgggcaa cctcgtccat gccgtctggg
     3901 agaaggtatg gcacgagagg atggatacag gggggaacct caccggtctt ttggaagact
     3961 gctgggatca ggcggtcgaa gagcgttacc cttccctgaa ggaattcccc aggcacatgt
     4021 ccaggcttaa gaagaatacc tggaggatgg catcactcca gcaggagatg gaagatgccg
     4081 ggcttgcgga ggccagggtc tcccaggagc gggagcagat gatagagacc gatctgaatg
     4141 gagttctttt ccgtggcaag ttcgaccgga tagaccttct gaaggatggc tcggcgatca
     4201 tcttcgacta caagacaggg cggggtaacg gccttgcagg ttcgatgcag cttccggcct
     4261 acgcagttct cctggagaag gcaaggaata taaagacatc ggggtatgcc ttcctctcgc
     4321 aggccgactg ttccgtcact ggccgcctgg aaggccccgc aggggtgatc cttgcccggt
     4381 ggaagaaaag gagccgaagc ggcctggctg acatggtctc cctggcttcg gagaccctcg
     4441 gcgccatgtc tgtgtcggtt acggaaagca gattcccccc tgactacacc aacaccgagg
     4501 cctgccggta ttgcgaattc caggagatct gccggagaaa agaatctccc gtaacccccc
     4561 ctgcagagga ggaaaaaggc gatgccgact gatatcttca gaaggtacct tgaagagacg
     4621 gcatgcctgg agcccaggca gatcaaggcc gtcatggccg acgaccccct ggtggtagtc
     4681 agcgccgggg cagggacggg aaagaccctg accctggcgt ggaggttcgt gaggctcgtg
     4741 gcggcgaacc agatccccgt cgacaggatc ctcaccatca cttttaccga gaaggccgcc
     4801 ctggaaatga gagagaggat acggcggctt atggaggaag tccgggacgg catccccgaa
     4861 ttatctcatc taatggacga tcccctttca cgactggagg atgcctacat atcaaccatt
     4921 catgccttct ccatgagggt ccttaccgaa tgcggcctca gcgccgatgt cgaccccggt
     4981 atcagggtga taaccccgcc tgaagagaac gccttctggc aatacctgga aaggtccatc
     5041 gaccgggaga caacgcagat ccttgcagaa accctcgggg gggaatggcg cgaaagggcc
     5101 gatgagatct tctctgccga aggaacagcc gacctggtga atgccttcgg ggcaggtgcc
     5161 atctgtgatg ctgcatcctc ggccatcccg cttttcgaaa gccgcggcct cgaccccgag
     5221 gcactatgga attgggccga cgaacttcca gaaagggact ctgaactttc aaccgggcta
     5281 ctggaggtct acacccctga atggcagaac acctgggaaa cctggatgaa aatggttctc
     5341 cccgaagccg ggggaccgga cattttcagg aaagacagca caaaattctc tgcaagggcg
     5401 tcggacttca tggagtcctg ggagaacagg ccagagaaga gtgaactccc ccgcttcgtg
     5461 atggccctcc tggacaagga aggcctgttg gggaacctcg ccggagccac gggcaagtgt
     5521 atgaaggccg tcgacgcggc ctccctctcg gtaaccgggg agagcctcaa ggattttcgt
     5581 aattcaagga taccctgggt cccggcggca cagtggatca tgaagggttt cactggcgcc
     5641 gagaccagac tccgcagtca cctcctgaag atcatcgcct tgtgctggag gcttttcgta
     5701 tccgcgaaag cggcaagggg caccttgtca ttcgacgaca tgatacgggg agcccgggac
     5761 gcggtatcag ccgaaccctc attcgccggg aaattccttc atgtgatcgt ggacgagttc
     5821 caggacacca attcaattca ggacgaactg ctaagcgccc tgaccccccc ggggggcacc
     5881 cttttcctgg tgggcgacct gcagcagtct atctaccgct tccggcatgc cgagccggct
     5941 atattctgga gaaggatcag ggaagccctg acccaaaacc ccgactcact gatctccctc
     6001 gacgtgacct ttcgctgcag acaaagcgtc atggacaggg tgaacagcct atttggccac
     6061 acctggagag aggggatcgc aagatcgata aaaatggcat tctcccccct cgcccccccc
     6121 ggatctaggg attggtggac caagcgccag gaaaccactg taagcccctt cgaactggtg
     6181 atacccgaca caagagacct tgaagaggag ctgaacaccg ccgggatccg ggtcgccgct
     6241 ctgaggatac tttcggacag gatcctggag gccatcgata aaggcgccac ggtctgggac
     6301 tccgacggag ccggggggtt cgatgccagg cctgtttcat tccgcgactt cgcggtgctt
     6361 gtaccgacgc gggcattcta cgaccagatc gaacaggtat tcatcgagga aaggggtata
     6421 cctacctatt tcgagggtaa ccgcaactac ttcggcaggg gcgaggtacg cgacgctgtc
     6481 aatcttctcg aggcggcggc agaccccgat gataccctcg ccatgtcgtc attcctggca
     6541 tcaccgttga gcgggctgtc gctggaggtt tctgcagaac tgatatcccg gtcggtgagt
     6601 gaaagtgtct cattaagaaa attattttcc gaggaatgcc ctgacgatgc cgcaagattc
     6661 gaagacctcc gccggaccgg gctggcggca ggaccctccc ttgccctggc aaaactgctg
     6721 gataacattt cggtcatcct ttcatgtccc gcctggaaac ggccccggtt ggccgcgaac
     6781 ctccgaaggg cggtggacat ggcaagggag tacgaagcat ccatggggat gagcctctcg
     6841 gggtgcgctt catatctccg ctatatgacc gtcaagggaa ttgactccaa agaggcagac
     6901 accctgggag accaggacga catggtcagg gtaatgacgg tccactcggc aaaggggctt
     6961 gagtttcccg ttgttgccgt ggcaggtctt gagcaggggc tcaggaccag gagccagggc
     7021 atcaggctta tcccttcgcc gcacctgggg atagccgcca cggtcttccc cgacggctgg
     7081 agccccgata gggatgaaaa aacgctggca ggaacgatcc acgacatact tgagaccctt
     7141 gaggcccggg aggagaaaca gcgtctcctc tacgtggcaa ccaccagggc aagggattcc
     7201 ctgatcctcc tgggggcatg ccccatcgag ggaggcatcc ccagggcaag aaaacattcc
     7261 tggctggaga ccgtcaggga ctggatgggg atcgcgggag gcctcagcct ctccccggtt
     7321 gccgggagcg gggtttcagt tcccatcgcc tcgtccattc ccgcaacgac gtacaggggt
     7381 ggcaccgtaa ggctgccgga aagaacgggt gaaacccttg aaaggatcag cgccacggcc
     7441 tacgctctta taaggtactg tcccaacgcc ttcagaatga gacaccggca aggtctgggc
     7501 atttcatggg aactcaattc cgaaggtgca ggaggggctg acctcggaag ccttgcccac
     7561 tgga
//
LOCUS       DAOPKQ010000046         7546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10419_length_7546_cov_4.425577, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000046 DAOPKQ010000000
VERSION     DAOPKQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10419_length_7546_cov_4.425577"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK146_02645"
     CDS             complement(<1..428)
                     /locus_tag="PK146_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE91170.1"
                     /translation="MTENCLVRFSGVSYTYPEAAAPAIADIDLKIASGEWIAVLGCNG
                     SGKSTLAKHLNALLIPTQGDCFVNEFNTRGEDGKRSARRAVSMVFQNPENQIIAAVVE
                     EDVAFGPENLGLSPEEIRARVSWALDVSGLGKMARKPVYAL"
     gene            complement(425..784)
                     /gene="rplQ"
                     /locus_tag="PK146_02650"
     CDS             complement(425..784)
                     /gene="rplQ"
                     /locus_tag="PK146_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPE91171.1"
                     /translation="MRHRVDRRRLGRYGSHRLAMLSNLTASLFLEERVETTVTRAKEV
                     RRFAERMITKAKIGDLHNRRIVASRMGNVEAVKKLFNDIAGRFSERQGGYTRIIKTGY
                     RKGDAAPMAVIELVERS"
     gene            complement(781..1773)
                     /locus_tag="PK146_02655"
     CDS             complement(781..1773)
                     /locus_tag="PK146_02655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPE91172.1"
                     /translation="MEHIRPEIRIEESTPLSGRIVVGPLEKGYGVTLGNAIRRVLLSS
                     ISGAAITAVRIEGILHEFSTIPGMREDVIELLLNIKNVALKSFSGGTKVLRLEAKGPC
                     EVTPENIIPDSDIEFVDPKAYICTLEDGASIEMDLFVEQGTSYVTSERERPSDLPLDA
                     LVVDAVFSPVRRARYEVQDARVGQRTDYERLVLEVWTNGSIAPEKAVIDAASILQRSF
                     GMVVQGLSKDESEGTDDTHSDGSAGDKRDADGVLMRSIKDLELSIRSENCLLRGGVQT
                     IGDLMSRSRSELLKIRNLGKISLREIEEKLEPFGLDISGDDHSSEEKMKEDEAR"
     gene            complement(1795..2424)
                     /gene="rpsD"
                     /locus_tag="PK146_02660"
     CDS             complement(1795..2424)
                     /gene="rpsD"
                     /locus_tag="PK146_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016801712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPE91173.1"
                     /translation="MSRYTGAVCRLCRTEGTKLFLKGDRCYTEKCAINKRNFKPGQHG
                     QNRRTKVSEYGIRLREKQKLRRFYGINETQFRRFYAAASKMAGQTGHNFLQLLERRLD
                     SVVYRFGIGVSRNQARQFVRHGHITVNGRKVDIPSFLVKTGDVITVHEGSRDKVLIKE
                     NIEVAASRTIPEWLQFDAEKHEGRILALPNREQIEVPVKEQLVVEFYAR"
     gene            complement(2451..2843)
                     /gene="rpsK"
                     /locus_tag="PK146_02665"
     CDS             complement(2451..2843)
                     /gene="rpsK"
                     /locus_tag="PK146_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPE91174.1"
                     /translation="MAKRTQRRGKKKERKNISYGVAHIYSTFNNTIISVTDRQGNLLS
                     WASGGNVGFKGARKSTPFAAQMAAQQVAKQVQDHGVQEIDVVVKGPGPGRESAIRSLQ
                     AAGLQVNAIKDETPIPHNGCRPPKRRRV"
     gene            complement(2890..3273)
                     /gene="rpsM"
                     /locus_tag="PK146_02670"
     CDS             complement(2890..3273)
                     /gene="rpsM"
                     /locus_tag="PK146_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPE91175.1"
                     /translation="MARIAGVDLPREKRIEVALTYIYGIGLPSSRKILEATSIDPDTR
                     VKDLTEEEAQKLRAEIEEAYRVEGDLRREVTMNIKRLIEIGCYRGMRHKFGLPVRGQK
                     TKTNARTRKGPRRAVAGKKKPAAKK"
     gene            complement(3281..3406)
                     /gene="rpmJ"
                     /locus_tag="PK146_02675"
     CDS             complement(3281..3406)
                     /gene="rpmJ"
                     /locus_tag="PK146_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPE91176.1"
                     /translation="MKVKPSVKPICEYCRVIKRNGIVRIICSKNPRHKQRQGARR"
     gene            complement(3482..3700)
                     /gene="infA"
                     /locus_tag="PK146_02680"
     CDS             complement(3482..3700)
                     /gene="infA"
                     /locus_tag="PK146_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPE91177.1"
                     /translation="MPKDDMIEVKGKVIEPLPNAMFRIELDNGHRILGHVSGKMRMHF
                     IRILPGDKVLVQISPYDLTRGRIVYRYK"
     gene            complement(3864..4634)
                     /gene="map"
                     /locus_tag="PK146_02685"
     CDS             complement(3864..4634)
                     /gene="map"
                     /locus_tag="PK146_02685"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPE91178.1"
                     /translation="MVSLKNEKDIECLRKAGKIVVDALNLVRDNIRPGVDTLTLDEKA
                     EKLITDSGARPAFKGYTVPGVPKPFPGTLCISINNEVVHGIPSGDRVLEEGDIVSIDV
                     GVRIDGFYGDAAYTFPVGRISPEREALLKVTRECLERAISAANEGATLGDIGHAVESW
                     ATPRGYGIVRNYAGHGIGRNLHEAPQIPNFGVPGRGITLKKGMSLAIEPMIMTGKEAV
                     RTLKDKWTVVTADGSDAAHFEKTIVITGKDPEVITPWE"
     gene            complement(4638..5282)
                     /locus_tag="PK146_02690"
     CDS             complement(4638..5282)
                     /locus_tag="PK146_02690"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPE91179.1"
                     /translation="MKVIFLGSPGAGKGTQAAILRERYGLAHISTGDILRDNVRNGTT
                     LGRTAREYMDSGRLVPDEVIVAMMEERLQQPDCQKGFILDGFPRTVPQAEALEALLDR
                     MGISLDGVVLFEVPGNLVVDRLTGRRVCGKCGEIYNVHFKKTRVPGVCDLCGGELVQR
                     DDDREEVVIKRLEVYEEQTAPLIRYYEEKGQLFRVDAGQEGELVVTAIERAVGQ"
     gene            complement(5298..6593)
                     /gene="secY"
                     /locus_tag="PK146_02695"
     CDS             complement(5298..6593)
                     /gene="secY"
                     /locus_tag="PK146_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPE91180.1"
                     /translation="MLDAFRDAFRLPDLKRKILFTMGVLFLFRLGTHIPTPGIDAKAM
                     ASLFEQGGVLGFLDLFAGGALRRFSVFALGVAPYINASIVMQLLVVVFPALEKLQKEG
                     EQGRKKIVQYTRLGTVFFAAVQAIGMTFWLRNLGVFSGGWMGFTAAVFTVTAGSLIVM
                     WLGEEISDHGIGNGISLLIFAGIVARLPEAIIRTWTLLRMGEMNILVVTLAIVLMLVV
                     IAGCVLLQEGQRRLPVQYAKRVVGNKVYGGQSTFIPLKVNQAGVIPIIFASSVLLFPY
                     TIVRFFTGNVSMMIQRFLSPDSLVYMTLYVLLIIFFSYFYTAVVFDPNDTANNMKKYG
                     GFVLGIRPGKPTAEYIEKIMSRITLGGAVFLAGVALLPTLMTRLMGITTFYFGGTAVL
                     IVVGVALDTVHQIEAQLLMRHYEGILKRSKGKTAGLLKF"
     gene            complement(6594..7040)
                     /gene="rplO"
                     /locus_tag="PK146_02700"
     CDS             complement(6594..7040)
                     /gene="rplO"
                     /locus_tag="PK146_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPE91181.1"
                     /translation="MGIHELRPAPGSKKKPKRLGQGVGTGKGKTAGKGNKGQKARSGG
                     GVRPGFEGGQMPLSRRTPKRGFSNARFKKTFQLVNVERLEELFENGSKVGIPEMIMAG
                     LVRSGKKPVKVLGDGEVSKALTVKANAFSAQAVAKIEAAGGKTEVI"
     gene            complement(7027..7239)
                     /gene="rpmD"
                     /locus_tag="PK146_02705"
     CDS             complement(7027..7239)
                     /gene="rpmD"
                     /locus_tag="PK146_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPE91182.1"
                     /translation="MAKLRITLKKSTIGRPPRQGQTVRALGLRKIRQSVVHEDNPQIR
                     GMVAAIEHLVDWEIVEEQGGEARGNS"
     gene            complement(7254..>7546)
                     /gene="rpsE"
                     /locus_tag="PK146_02710"
     CDS             complement(7254..>7546)
                     /gene="rpsE"
                     /locus_tag="PK146_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162817.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPE91183.1"
                     /translation="ELKRVGSSIPHDIVGHFGAAEVLLKPAAPGTGVIAGGAVRAIME
                     LGGVSDVLTKVIGRTSNPINVAYATMKALKGLRTPAEVMRLRGKEAPQAQEA"
BASE COUNT         1369 a         2367 c         1997 g         1813 t
ORIGIN      
        1 agggcgtaga cgggtttccg ggccatcttt cccagacccg aaacatccag ggcccagctg
       61 accctcgcgc ggatctcctc cggggagagg cccaggttct cgggaccgaa ggctacgtcc
      121 tcctctacca cagcggctat gatctggttc tcggggttct ggaagaccat ggagacggcc
      181 cgccttgcgg accgtttgcc atcttctcct cgtgtattga attcattgac aaaacaatcg
      241 ccctgcgttg gtatcagcag ggcgttgagg tgtttggcca gggtcgattt ccctgatccg
      301 ttgcaaccca gtacggctat ccattctccg gaggctatct tgaggtcgat atccgcgata
      361 gctggggccg cagcctccgg gtaggtgtag gaaaccccgg aaaaccgtac cagacagttt
      421 tccgtcatga cctctctaca agttctatta cggccatagg ggcggcgtct cccttgcggt
      481 accctgtctt gattatgcgg gtataccccc cctgcctctc gctgaaacgt ccggctatgt
      541 cgttgaagag tttcttgacg gcttcgacgt tacccatcct tgaggcgacg atcctccggt
      601 tgtggaggtc gcctatcttg gccttggtta tcattctttc ggcgaaacgg cgcacctctt
      661 tggccctggt aacggtggtc tccacgcgct cctccaggaa caggcttgcc gtgaggttgg
      721 aaagcatggc cagccgatgg cttccgtagc ggcccagcct gcgtctgtca acgcggtgcc
      781 tcatcgggct tcatcctcct tcatcttttc ctctgacgag tggtcatccc cggagatatc
      841 cagcccgaag ggttccagct tctcttctat ctctctgagg gatatcttgc ccaggttcct
      901 tatcttcagg agttccgacc ggctccggct cataaggtcc cctatggtct ggaccccgcc
      961 gcgaagcagg cagttctcgc tcctgatgga aagttccagg tcctttatgg acctcatgag
     1021 gaccccgtcg gcatcccttt tgtcacctgc ggatccatcg ctgtgggtat cgtccgtccc
     1081 ttccgattca tccttgctga gaccctgcac caccatgccg aaggatctct ggaggatgga
     1141 ggcagcgtct ataacggcct tttcgggcgc gatagaaccg ttggtccaga cctcaaggac
     1201 aagcctctcg tagtcggtcc tctgtccgac ccttgcgtcc tggacctcgt acctggcacg
     1261 gcggaccggc gagaacacag catccaccac aagggcatcc agcggaagat cggagggcct
     1321 ctccctttcg gaggtaacgt agcttgtacc ctgttccaca aagaggtcca tctcgatgga
     1381 ggctccgtct tccagggtgc atatgtaggc cttcgggtcc acgaactcga tgtcgctgtc
     1441 cgggattatg ttctcgggtg taacctcgca gggtcccttc gcttcgagcc tcagcacctt
     1501 cgtccccccg ctgaagctct tgagggcaac gttcttgatg ttgaggagga gctcgatgac
     1561 gtcctctctc ataccgggta tggtgctgaa ctcgtgaagg atcccctcta ttctcacagc
     1621 cgtgatggcc gccccgctta tcgaggacag caggacccgc ctgatcgcgt tcccgagggt
     1681 aacgccataa cccttttcaa gagggccaac gacgatcctt ccgctaaggg gggtgctttc
     1741 ctctattcgg atctcgggtc tgatatgttc catcattctc cccccagtca ttccttatcg
     1801 ggcatagaac tctacgacca gctgttcctt gaccgggacc tcgatctgtt cacggttcgg
     1861 gagggccagt atgcgccctt cgtgcttctc ggcatcgaac tggagccatt ccgggatggt
     1921 cctggaagcc gccacttcga tgttctcctt gatgaggacc ttgtcccggc taccctcatg
     1981 gacggtgatc acatcgccgg tcttcacgag gaagctgggt atatcgacct ttcggccgtt
     2041 caccgtgatg tgaccgtgcc tgacgaactg cctggcctgg ttccggctga cgccaatgcc
     2101 gaagcggtag accacgctgt cgagacgccg ctcgagaagc tgtaggaagt tgtgaccggt
     2161 ctggccggcc atcttcgagg ccgcagcata gaaacgcctg aactgggtct cgttgatacc
     2221 gtagaagcgt ctcagcttct gcttctcccg gagacggata ccgtactcgc tgacctttgt
     2281 cctgcggttc tgtccgtgct ggcccggttt gaagttcctc ttgttgatag cgcacttttc
     2341 cgtataacac ctgtcgccct tgaggaagag tttagtcccc tcggtcctgc acagacgaca
     2401 gacggcacca gtgtatctgc tcatttccat tgtcctcctt gatcagcccg ctagactctc
     2461 cggcgcttgg gaggccggca accgttatgg gggatcgggg tctcgtcctt gatggcgttc
     2521 acctgcagcc ccgcagcctg gagcgagcgg atggccgatt cgcggccggg accggggccc
     2581 ttgacgacca catcgatctc ctggacaccg tgatcctgca cttgcttggc aacctgctgg
     2641 gcagccatct gtgctgcgaa gggagtcgat ttcctcgccc ccttgaaacc tacattgcct
     2701 ccggaagccc atgagagaag gttcccctgt ctgtcggtga cactgattat ggtgttgttg
     2761 aaggtagagt agatatgggc aacaccatag ctgatattct ttctttcctt tttcttgcct
     2821 ctgcgctgtg ttctcttcgc cacgcgaata ttccctccct tgccttatcg ggacaccctg
     2881 ttaagggggt tatttctttg ccgcgggctt cttcttgccc gcaactgccc tgcggggtcc
     2941 cttgcgggtc ctcgcgttgg tcttcgtctt ctggcccctc acgggaaggc cgaacttgtg
     3001 gcgcattccc ctgtagcagc ctatctcgat gagacgcttg atgttcatgg tgacctctcg
     3061 cctgaggtcg ccttcgaccc tgtatgcttc ctctatctcc gccctgagct tctgggcctc
     3121 ctcctcggtg agatccttaa ccctggtgtc ggggtctatg gaggtcgcct caaggatctt
     3181 cctggaggac ggcagaccga tcccgtagat ataggtcaga gcgacctcga tacgcttttc
     3241 ccggggaaga tcgactcctg cgattcgtgc catgggatta ctacctcctt gctccctgtc
     3301 tctgtttgtg tctcggattc ttgctgcaaa ttatcctgac gatcccgttc ctcttgataa
     3361 ccctgcaata ttcgcagatg ggttttaccg aaggtttcac tttcatttga ttccacgctc
     3421 ctcaaaacag ctgaatagct caccgattat tgtatacttc tgtgactttt cagagtccaa
     3481 actatttata tctatacacg atccttcctc gtgtcaagtc gtagggtgat atctggacca
     3541 ggaccttgtc gcccggaagg atccttataa aatgcatccg catcttcccc gacacgtgcc
     3601 ccaggatcct gtgaccgtta tccagttcga tccggaacat ggcgtttggc agaggttcta
     3661 tgacttttcc ctttacttcg atcatgtcat cttttggcat cggctccttc accctccctg
     3721 tcgcacctgg cgagtcgtct tgatccggtc agtgccgggt cccggagcct cagccgccct
     3781 gggtcccgcg gatcccgcag tcgccggacg ggcgtctgca tcaaaaggcg ctccagcttc
     3841 aaggaagctg ccgtccaggc ttcttactcc caaggcgtga tcacctccgg gtctttcccg
     3901 gtaatgacga tggtcttctc gaaatgggcg gcatcggagc catcagccgt taccacggtc
     3961 catttatcct tcagtgtcct gaccgcctct ttgccggtca tgatcatagg ctctatggcc
     4021 agggacatcc ccttcttaag ggttatgccc ctgcctggga ccccgaagtt cgggatctgc
     4081 ggtgcttcgt gaaggttccg tccgatcccg tgacctgcat agttcctcac gatcccgtaa
     4141 cctcttgggg tcgcccagga ttcgacggca tggccgatat caccaagggt cgccccctcg
     4201 ttggccgccg aaatggccct ctccaggcac tccctggtaa ccttgagaag ggcctccctc
     4261 tccggcgata tcctgcccac cgggaaggta taggctgcat ccccgtagaa cccgtcaata
     4321 cggaccccaa cgtcaatgct gacaatgtca ccctcctcaa ggaccctatc cccgctgggt
     4381 atcccgtgga caacctcatt gttgattgat atgcaaagcg tcccggggaa gggcttggga
     4441 acgccgggga cagtgtaccc cttgaaggcc ggacgggcgc ccgaatcagt gatcagcttc
     4501 tcggctttct cgtccagcgt cagggtgtcc actcccggac gtatattgtc tctgacaagg
     4561 ttcagggcat cgacgactat ctttccggcc ttcctgagac attcgatgtc cttttcgttc
     4621 ttgagggata ccatgatcta ctgccctacg gccctttcaa tggccgtgac gacaagttcc
     4681 ccttcctggc ctgcgtcaac ccggaaaagc tgccccttct cctcgtagta gcgtatcagg
     4741 ggagccgtct gctcttcgta gacctccagc ctcttgatga caacctcttc cctgtcgtca
     4801 tccctctgga ccagttctcc gccgcacaga tcacagaccc cgggaaccct ggtcttcttg
     4861 aaatggacat tgtagatctc gccgcatttc ccgcagaccc ttctgccggt caggcggtct
     4921 accaccaggt tgcccggtac ctcgaagagc acgaccccgt caagggagat ccccatcctg
     4981 tcaagaaggg cttccagggc ttcagcctgg gggaccgtcc tcgggaatcc gtcgaggatg
     5041 aaccccttct ggcagtccgg ttgctggaga cgctcctcca tcatggcgac tatcacttcg
     5101 tcggggacca gcctgcccga gtccatgtac tccctggcgg tcctgcccag agtggtcccg
     5161 ttcctgacgt tatccctgag aatgtctccc gtggatatat gggcgagacc gtaacgctcc
     5221 ctgaggatcg ccgcctgtgt ccccttgcct gcgccgggtg atccgaggaa tataactttc
     5281 atggccctgt catcccccta gaacttgagg agcccggcgg tctttccctt ggaccgcttc
     5341 aggatgccct catagtgtct catcaggagc tgtgcctcta tctggtgaac cgtgtcgagg
     5401 gcgacgccga ctacgatcag gactgctgtc cctccaaagt agaaggtggt tatccccatg
     5461 agccgggtca tcagcgtcgg caaaagtgcc acgccggcaa ggaagaccgc gccaccgagg
     5521 gtgatcctcg acatgatctt ctcgatgtac tccgcagtcg gtttgcccgg cctgataccg
     5581 agaacgaacc ctccatactt cttcatgttg tttgccgtgt cgttgggatc gaaaacaacg
     5641 gccgtataga agtaggagaa gaagataatg agcagcacgt agagcgtcat gtagacaagg
     5701 ctgtcaggcg aaagaaagcg ctgtatcatc atcgatacgt tccccgtgaa gaaacggacg
     5761 atcgtatagg ggaacagcag caccgacgac gcgaatatga tcggtatgac ccctgcctgg
     5821 ttgaccttga ggggtatgaa ggtgctctgg cctccgtaga ccttgttccc gacgaccctc
     5881 ttggcgtact ggacggggag ccttctctgc ccctcctgga ggaggacaca cccggcgatg
     5941 acaacgagca tcagcacaat ggccaaagtc accaccagga tgttcatttc acccattctc
     6001 agaagcgtcc aggtcctgat aatggcctca ggcaacctgg cgacgatccc cgcgaatata
     6061 agaagagaga tgccgttgcc gataccatgg tctgagatct cctcaccaag ccacatcacg
     6121 atcagggaac cagccgtgac ggtgaagacc gcggcggtga agcccatcca tcctcccgag
     6181 aatacaccca ggttcctcag ccagaaggtc atcccaatgg cctggacggc ggcgaagaac
     6241 accgtgccga gccgggtgta ctggactatc ttcttcctgc cctgttcccc ttccttctgg
     6301 agcttctcaa gggcagggaa caccactacc agcagctgca tgacgatgct ggcgttgatg
     6361 tagggagcta ctcccagagc gaagacgctg aatcttctga gggccccccc ggcgaagagg
     6421 tcgaggaaac cgagaactcc gccctgttcg aacaggctgg ccatggcctt ggcgtcaata
     6481 cccggcgtcg ggatatgagt accaagacgg aagaggaaga ggacccccat ggtgaagagg
     6541 atcttcctct tgagatcggg caatctgaaa gcatccctga atgcatccag cacctagatc
     6601 acctcggtct tcccgcctgc agcctctatc ttggccactg cctgggcgct gaaggcgttg
     6661 gccttcacgg tcagtgcctt ggaaacttca ccgtccccga ggaccttcac aggcttcttg
     6721 ccactccgga caaggcccgc cattatcatc tcgggaatac ctaccttgct gccgttctca
     6781 aaaagttcct ccagcctctc tacgttcacc agctgaaagg tcttcttgaa ccttgcgttg
     6841 ctgaaacctc tcttgggggt cctcctcgaa aggggcatct gcccgccttc gaaaccggga
     6901 cggacccctc cgccgcttct ggccttctgg cccttgttcc cctttccggc agtcttgccc
     6961 ttgccggtcc cgacaccctg ccccaatctt ttaggttttt tcttcgaccc aggagcaggc
     7021 cggagttcat gaattcccac gagcctctcc cccctgttct tctactatct cccagtccac
     7081 caggtgctct attgcggcga ccatccctcg gatctggggg ttgtcttcat gtacgacaga
     7141 ctgcctgatc tttctcagtc ccaatgcacg gaccgtctgc ccctggcggg gaggccggcc
     7201 tattgtgctc ttcttcaggg ttatcctaag cttggccatt gtcgttcagc cccctacgct
     7261 tcctgggcct gaggcgcttc cttgccccgg agtctcatta cctcggccgg agtgcggagt
     7321 cccttcagcg ccttcatggt ggcgtatgcc acgttgatag ggtttgaggt cctgccgata
     7381 accttggtca gtacgtcact gacgccacca agctccatga tggcccggac agcaccaccg
     7441 gcgataactc ccgttccggg ggcagccggc ttgaggagga cttcggcagc tccgaaatga
     7501 cccactatgt catgggggat gctgctgccg accctcttca gttcga
//
LOCUS       DAOPKQ010000047         7494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10504_length_7494_cov_4.489313, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000047 DAOPKQ010000000
VERSION     DAOPKQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10504_length_7494_cov_4.489313"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /gene="obgE"
                     /locus_tag="PK146_02715"
     CDS             complement(<1..1325)
                     /gene="obgE"
                     /locus_tag="PK146_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPE91184.1"
                     /translation="MRFLDSAEISVRAGKGGNGALSFRREKYVPRGGPDGGDGGRGGH
                     VLIVADDHIQTLADYEYRRRFIAGNGGNGRGRNQTGPSGEDVLVKVPCGTIVNERGTG
                     RFLADLTVAGDTLLAARGGRGGRGNARFTSSKRRSPRFSEMGGEGEARDLVLDLKVLA
                     DVGLVGLPNAGKSTLLSAISGSRPQIAPYPFTTLSPNLGMMDFDNRRMVVADVPGLIE
                     GAHMNRGLGHAFLKHVERTRLLVYVIDLSEEAPVFPIDQWNTLRSEFQAFNPELLSLP
                     SVVAGNKTDLPYRTTQTRDLQKVLKAEGVPFFPISALSREGVDGLRDHIASRVSEQAA
                     SRRESHFIVIEDEEDRTGHRAEKPRIEKFLEKGESIFIVRHYEVERLLRKFNFDQEEA
                     LGRFSAILRYYRVEDLLLESGAREGDTVRIGETEFEFYPEVSSTLKDRT"
     gene            complement(1402..1683)
                     /gene="rpmA"
                     /locus_tag="PK146_02720"
     CDS             complement(1402..1683)
                     /gene="rpmA"
                     /locus_tag="PK146_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPE91185.1"
                     /translation="MRTINLQLFAHKKGQGSSTNGRDSIGKRLGIKRNDGQVVTAGSI
                     IVRQRGTKYHPGENVGRGRDDTLFALSEGTVRFHTRAGRKYVVIEPVTA"
     gene            complement(1649..2008)
                     /locus_tag="PK146_02725"
     CDS             complement(1649..2008)
                     /locus_tag="PK146_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HPE91186.1"
                     /translation="MTEIRSFRRKGNIFGLDVSGHTGYAEHGADIVCSAISTLVQTLE
                     TGLTEIIRIEGVKSSIDEDRGSMSLFWHGSEDSRVRTLVDTVIEVLKSVEKSYPSYVK
                     FMEVEVDENDQSSTVRS"
     gene            complement(2017..2331)
                     /gene="rplU"
                     /locus_tag="PK146_02730"
     CDS             complement(2017..2331)
                     /gene="rplU"
                     /locus_tag="PK146_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPE91187.1"
                     /translation="MYAVIETGGKQYRVSPGDTIRIEKVSAAEGEEVEFSKVLLVATD
                     SGVSLGEPTISGALVKGHVLEHGRSKKITVFKYKNKSRQSRRTIGHRQAFSSVRIDSI
                     IC"
     gene            complement(2497..4158)
                     /locus_tag="PK146_02735"
     CDS             complement(2497..4158)
                     /locus_tag="PK146_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE91188.1"
                     /translation="MLSELMVTNVGGVREASLHFRGRFIVITGESGAGKSSLVRALEL
                     VSGKRAQSSIIRAGVEEAEVRAALEMDAPLSSLPEEFQPQEGFILVKRVVSSTGRAKS
                     YLQEKPVPLNTLAGALSKVVAIQSQFAQLELLEPEQQVELLDHSGGEILREIRSNLSM
                     AVSSAIENERKLLEVSRKRREIEARYQDGEAFLEKYRRMEIRPGCESEWETDLNRVSG
                     LLKRQAGLRLLHDRMTGGSSGEGLSAAIEQFGQDIRQSVDGAPELEVNIERLLTASQE
                     IEKALKAFVSIEREQSLQEEFEKIEKKIGTLRKLMRYAKVDSAEDLVDFADRLDLDLQ
                     WLRESREKLALMQENSACLKKEVSNLALSLREARKNAARRLEESVNASLGSLAMEGLR
                     FGITLKPLEKVRQSGADDISFFLSDGKHLQGPVSRIASGGELSRILLALQIAASDDML
                     PRTLVFDEVEAGLGGRSAFLAGQALRELSRRCQVVLITHEASIASLADQHFRVEREGD
                     NSQVLEVDGKQRVRELARMLAGDPDDPEATDLARSLLEGNRRETG"
     gene            complement(4163..5041)
                     /locus_tag="PK146_02740"
     CDS             complement(4163..5041)
                     /locus_tag="PK146_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPE91189.1"
                     /translation="MIETLGLLVNTQKRSALALAEKILRWGRERSVKFVLPPHEAHVL
                     GEQETSDHDWKEKVDAAVVLGGDGTFLRAARYVIGHDIPLYGINVGRLGFLSCGKIEN
                     AEKDLEKIVTGKLTVNHRPLLHGIVSRGKRHKHEVYALNDLVVTKGPFARLIALNIAV
                     SGRPLSLLHADGIIIATPTGSTAYSLSAGGPIVPPHMSCMILTPICAHTLYSRPIVLS
                     ESDTVTITPEGQHREIMLTQDGQLGYELLPGDTISISLDKDRNIGIITLPENEFFSLL
                     QEKFQWGHRTPNGSGA"
     gene            complement(5041..5862)
                     /locus_tag="PK146_02745"
     CDS             complement(5041..5862)
                     /locus_tag="PK146_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPE91190.1"
                     /translation="MVVRGITESRSRAQSLILAGSVRVDGEKVLKPGKKVLRDSVIEA
                     DKGPGWASRGAFKLLKALDSFCVEPSGKVCIDIGASTGGFTDVLLERGAVKVYSVDVG
                     YGQLAWRLRNDERVVVMERTNARDLRSRDLEDAPGVAVMDVSFISVTRILPALEGLLA
                     PGGDCIALIKPQFEAGKGCVGKKGVVRESSVHLGVLNTLGAFISSMEEMTLMGADFSP
                     ILGPSGNMEFLFHIRKGGIPRVVMDKGSFGDLVERAHRELLGVFLEKTDRTEGAL"
     gene            complement(5981..7494)
                     /gene="dxs"
                     /locus_tag="PK146_02750"
                     /pseudo
     CDS             complement(5981..7494)
                     /gene="dxs"
                     /locus_tag="PK146_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
BASE COUNT         1502 a         2295 c         1880 g         1817 t
ORIGIN      
        1 tttgtcctgt ctttcagtgt cgacgacacc tccggataga actcgaattc ggtctcccct
       61 atccttacag tatcaccttc acgagccccg ctctcaagga gcaggtcttc tacacggtag
      121 tatctcagga tcgcggagaa ccttcccagg gcctcttcct ggtcgaagtt gaacttccgg
      181 agaagccttt cgacctcgta atgtcggaca ataaagatgc tctcgccctt ttcgaggaat
      241 ttctctatcc tgggcttctc cgccctgtgc ccggtcctgt cctcctcatc ctctatcaca
      301 atgaagtggc tctctctcct tgatgcggcc tgttcagaga ccctcgatgc tatgtggtcc
      361 ctcaatccgt cgactccctc ccgggacagg gcacttatgg ggaaaaacgg cactccctct
      421 gccttcagca ccttctgaag gtcccgggtc tgcgtggtcc ggtaagggag atctgtcttg
      481 ttgccggcaa cgacggaagg aaggctgagc agttccgggt tgaaggcctg gaactctgat
      541 cttagcgtat tccactggtc aattggaaag acaggagctt cctctgaaag atcgatgacg
      601 tagacaagga gccgggtcct ctccacatgc ttgaggaagg catgacccag ccccctgttc
      661 atgtgggccc cctcgatcag tcccgggaca tcggccacga ccattctcct gttatcaaag
      721 tccatcatgc ccagattagg ggagagtgtt gtgaaggggt aaggggctat ctggggcctt
      781 gagcctgaaa tggccgaaag gagcgtcgat tttccggcgt tcggaagacc gacgagtccc
      841 acgtcggcca gcaccttcag gtcaaggacc aggtccctgg cttctccctc ccctcccatt
      901 tccgagaaac gggggcttct tcttttcgaa gaggtgaacc ttgcgttccc tctgcctccc
      961 ctgccgcctc ttgcggcaag aagtgtatca ccggcaacgg taaggtcggc caaaaacctg
     1021 cctgtccccc tttcgttcac gatggtaccg caggggacct tcaccagtac atcctcaccg
     1081 gagggacctg tctggttacg tccccttccg ttgcctccgt tcccggcaat aaaacgtctt
     1141 ctgtattcgt agtcggcgag ggtctggata tgatcatcgg ccacgatcag tacgtgtcct
     1201 ccccgccctc catcaccgcc gtcgggtcct cccctgggga cgtacttctc cctccggaag
     1261 ctcagggcac cattcccgcc cttgcctgcc ctgacggaga tctctgctga atcaaggaat
     1321 ctcaatacct gcacccctca aatacggaaa aagggcccaa gatcaactca ggccctgcgg
     1381 cgttcctatc ggacagaagc atcaggcggt cactggttct atgacaacgt attttctccc
     1441 tgccctggta tggaagcgca ccgtgccttc tgaaagggcg aaaagggtat cgtccctccc
     1501 tctccctaca ttttctcctg ggtggtactt ggtccccctc tggcggacga taatgctccc
     1561 ggcagtgact acctgcccgt cattcctctt gatcccgagc cttttgccga tgctgtccct
     1621 tccgttggta ctgctgccct gtcctttttt atgagcgaac agttgaagat tgatcgttct
     1681 catcgacttc cacctccata aacttaacgt aagaaggata actcttttct acagatttta
     1741 gaacctctat taccgtgtcg accagggttc tgacccttga atcttccgat ccatgccaaa
     1801 agaggctcat ggacccccgg tcctcgtcaa tgcttgactt gactccctct attctgatga
     1861 tctccgtaag gcccgtttcg agggtctgca ccagggtaga tatggccgaa cagactatgt
     1921 cggcaccatg ttcggcatat cccgtatgac ctgaaacatc aagcccgaag atgttcccct
     1981 ttcttcggaa tgaccttatc tcggtcatct ttcgctctag cagatgatcg aatcgatcct
     2041 gacagaagaa aaggcctgcc tgtgtccgat cgtccttctt gactgccttg acttgttctt
     2101 gtacttgaag acggtgatct ttttcgatct cccatgttcc agcacgtggc cctttacaag
     2161 agctcctgaa atggtcggtt cgccaagaga gaccccgctg tcggtggcta cgagcaggac
     2221 cttggagaat tctacctctt caccttctgc ggcactgacc ttctcgatcc tgattgtgtc
     2281 tccaggggac accctgtact gctttccccc tgtttcaata actgcgtaca ttgacatttt
     2341 ccctcctaac gccggcgatc caggtggccc aaaggctctt gttgttaccc gtcccgagcg
     2401 aagctcaaaa gggaacggcc ggaagcctcc gccgtaaccc ctttcgtcga ccaggtcgac
     2461 cgaatggaga ttatagcata aaaatccctt gatcggtcaa ccggtctccc tccggttccc
     2521 ttcaagcagc gatctggcca ggtctgttgc ctccgggtca tcgggatcgc ctgccagcat
     2581 ccttgcgagc tcccttacac gctgttttcc gtcgacctca aggacctggc tattgtcacc
     2641 ctctctttcg acccggaagt gctggtcggc aagggaggcg atagatgctt catgggttat
     2701 aagtaccacc tggcaacgcc tggaaagttc cctcagtgcc tgacccgcaa ggaaagccga
     2761 acggcctcca agaccggcct ccacctcgtc gaagaccaga gtccggggga gcatgtcgtc
     2821 cgaggctgct atctggaggg ccagaaggat ccggctgagc tcgccccctg aagcgatccg
     2881 tgacaccgga ccctggaggt gcttgccatc ggaaagaaag aatgatatgt cgtcggctcc
     2941 gctctggcgg accttctcca gaggctttaa tgttatcccg aagcggagcc cctccattgc
     3001 aagggaaccg agggatgcat tgacggactc ctcgagcctc ctggcggcat tctttcgggc
     3061 ctctctgagg gaaagggcaa ggtttgatac ctctttcttg agacatgcag agttctcctg
     3121 catcagggca agcttctcgc ggctctctct gagccactga agatccagat ccagtctgtc
     3181 cgcaaaatcc acgagatctt ccgcactgtc gacctttgcg tacctcatga gttttctgag
     3241 ggtcccgatc ttcttctcga tcttttcgaa ttcttcctgg agggactgtt ctctctcgat
     3301 ggagacgaaa gctttcaggg ccttctcgat ctcctgggaa gcggttaaaa gtctttcgat
     3361 gttcacttca agttcagggg ctccgtccac gctctgccgg atatcctggc cgaactgttc
     3421 gatcgcggcg gaaagaccct ctccggagct accacctgtc atcctgtcat gaagaagcct
     3481 taatccggcc tgtcttttca ggaggccaga gacacggtta agatcggtct cccattcaga
     3541 ttcacaaccc ggccttatct ccatcctcct gtacttttct agaaaagctt ctccgtcctg
     3601 gtaccttgct tctatttccc ttctcttcct ggacacctcc agaagcttcc tttcgttctc
     3661 aatggcagac gaaacagcca tggaaaggtt cgacctgatc tccctgagga tctctccacc
     3721 actgtgatcg agcagttcga cctgttgttc cggctcaaga agctcaagtt gagcgaactg
     3781 gctctggatc gcgaccacct tggagagagc tcccgccagt gtattgaggg gtacgggttt
     3841 ctcctggagg tagctcttgg ccctccctgt cgatgagacc actcttttga caaggataaa
     3901 gccctcctgg ggctggaatt cttccgggag ggacgaaagg ggggcatcca tctccagggc
     3961 cgccctgacc tcggcttcct ccacacctgc cctgatgatc gaagactgcg ccctcttccc
     4021 gcttaccagt tccagcgccc tgaccaggct actctttcct gcaccgctct cccctgttat
     4081 tacgatgaac cttcccctga agtggaggga ggcttctcta accccgccta cattggtcac
     4141 catcagttca gaaagcatgt gatcacgctc cggaaccgtt cggggtcctg tgaccccact
     4201 ggaatttctc ctgtagaagg gagaagaatt cgttctctgg cagggtgatg atcccgatat
     4261 tacggtcctt gtcaagggat atggagatag tgtcgccggg gagaagctcg taccctaact
     4321 gtccgtcctg ggtaagcatg atctctctat gctgtccctc gggcgttatg gtcaccgtat
     4381 cggattcact cagcacgatg ggtctggagt atagagtgtg ggcacagatc ggtgtaagga
     4441 tcatgcagga catgtgagga ggcactatgg ggccccctgc cgagagagag taggccgtcg
     4501 atcccgttgg agtagctatg attatcccgt cggcatgcag aaggctcagt gggcgtccgc
     4561 tgacggctat gtttagagct atcagtctcg caaaaggccc cttggtcacg acaaggtcgt
     4621 tcagggcgta gacctcgtgc ttgtgcctct tccccctgct gacgataccg tgaagcaggg
     4681 gtctgtggtt tacagttaac ttccccgtca cgatcttctc aaggtccttc tcggcattct
     4741 ctatctttcc gcaggagagg aaacccagtc ttccgacgtt gatgccgtaa agagggatgt
     4801 catgccctat cacgtaacgc gcggccctca ggaaggttcc gtcccctcca aggacaacag
     4861 cggcgtcgac cttctccttc cagtcatggt ccgacgtctc ctgttccccg agaacatggg
     4921 cttcgtgggg ggggagtacg aacttgacac tacgttccct cccccacctg agaattttct
     4981 cggccagtgc cagggccgaa cgtttctggg tgttgaccag cagtcccagg gtttcgatca
     5041 tcaaagggcc ccctctgttc tatcagtctt ttcaagaaaa actcccagga gttccctgtg
     5101 agctctttca acgagatccc cgaaggagcc tttgtccatg actacccgag ggatccctcc
     5161 ctttctgata tggaacagga attccatatt accgcttggt cccagtatgg gagaaaagtc
     5221 agcccccatc aaggtcattt cctccatcga ggagataaat gccccgaggg tattgaggac
     5281 acccaggtga acggaacttt cccttacgac acctttcttg ccaacgcatc ctttgcccgc
     5341 ctcgaactgc ggcttgatca gggctataca atccccgccg ggggcaagca acccctcaag
     5401 ggcgggcagg atcctggtga cggatatgaa ggatacatcc ataacggcta ctcccggagc
     5461 atcctccaga tcccgggacc tcaggtctct ggcattggtc ctctccatga ccactaccct
     5521 ttcatcgttc ctcagccgcc aggcaagttg tccgtagccg acgtcgacgg agtagacctt
     5581 caccgctccg cgctccagaa gcacatcggt gaatcctccc gtggaagcac ctatgtctat
     5641 gcaaactttc ccggaaggtt cgacacaaaa ggaatccagc gccttcaaaa gcttgaatgc
     5701 ccctctgctc gcccatcctg gtcccttatc ggcttctatc actgaatctc tcaggacctt
     5761 cttccctggc ttcagaacct tttccccgtc cacccgtacg gatcctgcca gtatcaacga
     5821 ctgggcccgg ctcctgctct ctgtgatgcc ccttacgacc atttcctgat ccagtctttc
     5881 ctcttttcct gaagatctca tagagtccta tcacctcgcc gggaacaagt ccgcaatact
     5941 cccactgttc ccttaccgtt ccgtgaggga cgaaccggtc cgggactccc agggattcca
     6001 ccctgactgt cagtcctttc ctgtttgcaa gggaagagat cttctccccg ataccccctt
     6061 ctacgttact gtcctctatg accaccgcaa agaggtgtga agagagggcg gacattattg
     6121 tatcggtgtc aaggggtttc agaaaacgca ggtcatagac cgagggaggc tcaacgccgg
     6181 ttctccacgt ctcttcgtag gtccggaccg caagggggac cgtcgctcca taggaaaaaa
     6241 tacaccatcc cgtcccgtca aggatctttt cggatctccc ccactcccgg ggaaaatcct
     6301 gaccctcacg gcacatagat accgggacct gtccccgagg aagccttata gccgacgggg
     6361 accttgtttt cagcgcttcc tgatacatcc acctcaggtc atccctgtca cggggtgcca
     6421 ttactgcaag gttggggatt gtcctcgtcc agggaaggtc caggaccccg tgatgggtct
     6481 ccccgtcttc tcctaccaga ccagcccggt ctatggccag gatcacgtga aggtcctgca
     6541 tgcagatatc atgtaccaac tggtccatag ccctttgcag gaaggtggag tatacggaga
     6601 ccaccggaac catccccgcc gctgcgatcc ctgcggcaaa tgtcagaaga tgcccctccg
     6661 ctatcccgac atcgaagaat ctctcgggat acttctcgca gaagcggttc agtctgctcc
     6721 cttccttcat tgcggcagtc atcaggacca ctctcgggtc cctgtcggcc agctcttcta
     6781 cgcacgccgc agaagcttcg ctccagctct cttcgggtgt ggatgagggc atcatgtcga
     6841 gcatgcctgg cgacacaccg tggaacctct caggatcttc ctctgcgggc ctgaagccct
     6901 tgcccttgac cgtaagaagg tggatcagga gcggggactc gtagctcttg gccatatcga
     6961 atatcttctc catctcctgg atattgtggc cgtcaaaagg tccccagtag cttatgccaa
     7021 gatcctcgaa catgttgggg gggagaagaa ggcccttgac ggtctgcttg gacctctcaa
     7081 ggaaggattc aagccttctt ccgtcgggga ctttccggca aaagaacttg agaagcttct
     7141 tgaactgcct gtacatgggg ttcacgctca gtgcggcaag atggttggcc aggcccccca
     7201 ctcttctgtt aattgacatc tggttgtcat taagaatgaa caggacggga gttttcagtt
     7261 catgggcatg gttgagcgcc tccagggcaa gaccgttaat gagagatccg tcaccgataa
     7321 ctgccgccac agtgtgtctt cgccccaaga gatccctcgc tttggccagg cccagcgcgg
     7381 ccgacaggga tttgctgctg tgccctgcat tgaagtgatc aaaggggctc tccctcgggt
     7441 ccggaaagcc cgatacgccc ccccatctcc tgagtgtatg gaactggtcc tttc
//
LOCUS       DAOPKQ010000048         7411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10653_length_7411_cov_4.541735, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000048 DAOPKQ010000000
VERSION     DAOPKQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10653_length_7411_cov_4.541735"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PK146_02755"
     CDS             <1..581
                     /locus_tag="PK146_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPE91191.1"
                     /translation="GTFVSVSQCRDCNGSGKKIDSRCANCGGSGRVRTRHTVEVRIPP
                     GVDSGTRLRVAGEGMEGSQGGPPGDLFISIKVESHPLFERDRDDLHARLDLKYPQVVL
                     GASVEVPTPEGTDRIEIPPGTQGGKTFRVKGKGMPRLRGSGRGDLYAHVFIEVPRDLS
                     EKERTLVEALAQEMNVPIQSTGLMDRIRQLFS"
     gene            679..1566
                     /locus_tag="PK146_02760"
     CDS             679..1566
                     /locus_tag="PK146_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPE91192.1"
                     /translation="MSVEGFWWYITLEGQDGNEEDLSALADLSGSIGSEIKELPGRIA
                     LKAYFRSTRDLGFWIGRVQDVISPWPGVRIVDMGRIENRQWHTAWKDAFPPLGVGSRF
                     VVMAPWHKGKEPEGRTPLYIYPGSAFGTGYHESTQIALELLEHTVKPGMKVIDVGTGS
                     GILAVASLRMGASGVIATDNDPAIIPEMRENLRLNDIPDEAVDIRTGDLLEGIQGKVD
                     LVCANIVFDPLVSMIPVIPALLTEGGLAVFSGLTLKERDRFLEVLPSGGLAPTREIRK
                     GEWWGVASSCKAGVIGTGE"
     gene            1520..2245
                     /locus_tag="PK146_02765"
     CDS             1520..2245
                     /locus_tag="PK146_02765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPE91193.1"
                     /translation="MSLPRVRLESSVRESDTTWILDPREGHHLLSVRRCRDGDLFEGL
                     LPGRKILMRLDLSSGEARGVVISESPEGSGREIWLLAGLLKGDPFDRLVAQSVETGVS
                     VLVPLLCSRSVVSIPPGKITSRMERWRRIAMEATKQCGRADPPEILEPVPLSSIPYLQ
                     LPSHRFVTVTGPAQAILASRPHSRAAVAVGPEGDWTEEELVILEREGFCRVSLGPGVM
                     RSHTAGIVAVAVLSMMLDGDVHV"
     gene            2232..3551
                     /locus_tag="PK146_02770"
     CDS             2232..3551
                     /locus_tag="PK146_02770"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPE91194.1"
                     /translation="MSMYDRLRGVRVRILPMGCRTNLFESEALGCSFTEAGALLTDEE
                     VFDAAVVVTCSVTSEADRKCRQNLRRLRRENPDGIIIAAGCWAQRISPEEARDLGVDC
                     LVGNRLKFRIPGIVTEMIEHGRPTEGPRIERVDVQNLKEWDPLYLSARRTRTRAFLKI
                     QDGCDHRCAYCIIPDLRGMPVERHPGEILQEVRMLALSGCREVVFTGINLGMFGRTSG
                     VSLGDIVRRTGQVEGIERVRFGSLEPFCLTEDLLEALADTPQFCPHLHIPLQSGDPVI
                     LEKMGRGYTPGEFEALVGRARYFLGEDLHLSTDIIVGFPGETDKAFLSTLEMLERAGA
                     GKVHVFPFSLREGTPASLIDGRLPPRVVRSRMDQALSMASELLDRYAGRWVGEEVEIL
                     VEKSQGGVVQGLSSHFLRVRVPGEALQGEITTVVPGDTKKGVLLGMV"
     gene            3667..4008
                     /locus_tag="PK146_02775"
     CDS             3667..4008
                     /locus_tag="PK146_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPE91195.1"
                     /translation="MSRDCLFCRIAEGSERADIVYSDPDVIVIRDIHPRAPVHLLVIP
                     RQHVGSADEVEDPSVWSSLMNAAVRVARDMGLCAGGYRLVVNCGAGAGQTVFHLHVHL
                     LAGRVFQWPPG"
     gene            4040..4219
                     /gene="rpsU"
                     /locus_tag="PK146_02780"
     CDS             4040..4219
                     /gene="rpsU"
                     /locus_tag="PK146_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPE91196.1"
                     /translation="MTQVVRRDNESLEDALKRFKREVSKGGILREARRRKHYEKPSEA
                     KKRKREDASRKRRGK"
     gene            4227..4688
                     /locus_tag="PK146_02785"
     CDS             4227..4688
                     /locus_tag="PK146_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPE91197.1"
                     /translation="MTGLEERIHLEMVKAMKARDQSSLVPLRMLKAALDNTRVEKGRS
                     GGSLSDEDILSVVRRLVKQRKDAAEQFSAVGALGRAEEERQEADLLSSFLPPSMDDGT
                     ILDVVRSVAAETGAKGPSDLGRVMGRTMAQLKGKAEGERIRGIAARFLDTL"
     gene            4814..5284
                     /gene="nrdR"
                     /locus_tag="PK146_02790"
     CDS             4814..5284
                     /gene="nrdR"
                     /locus_tag="PK146_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPE91198.1"
                     /translation="MRCPRCGLMETRVLETRTASEGRVIRRRRECPGCMNRFTTYERL
                     EDRPVLWIVKKSGQREAFDREKLLKGFAKACEKLPVPLEQIEEAVSRIEETLRETGQG
                     EVSSMTVGSLAMMELRDIHKVAYVRFASVYREFTDVSSFVEEISRLRDERNGQR"
     gene            5350..>7411
                     /gene="nrdD"
                     /locus_tag="PK146_02795"
     CDS             5350..>7411
                     /gene="nrdD"
                     /locus_tag="PK146_02795"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPE91199.1"
                     /translation="MLIFSGRGETTDLALMVEALAQEDRSPWNAYRIRDALIREAGVD
                     PATAEEIALEVEADILRHGKERVTTSTIRELVNVKLFFRGLDAKLADHSRLGLPVYDL
                     ESMMLSPNRENSNTSHNPESINLSIAEMILKEYALKKVFPGEVARAHLAGDIHLHDLG
                     MVNRPYCSGQSMAYVIRYGLDLPSITSVSAPARHPDVLLAHILKMTSVLQNNFAGAIG
                     WDAVNVFFAPFLTGLPYEKVRQLAQMLIFEFNQLAGGRGGQVAFTDINLYYEIPDHFR
                     EVQALGPGGLPTGRTYGQYSEESKLFLRALFEVYLEGDSRGQPFFFPKPLLHITDRFF
                     QEPGWEEFLRFACLVASEKGSTYFVFDRGGVAKLSECCRLSFELSEDDLREAATPWKM
                     RYCALQNVTINLPRIAYRAGGDRDSLFEILDRMMEAAALAHVRKREFLSEMLALGSKG
                     PLAVLCVDHDGETYIRLKKSSYLIGLIGLEEMVHCVTGKRMHEDREAMDLGLAVTRYM
                     DLKCRELTERLGLKIVLEQTPAESTAYRLARLDLKFFPDQASMFVKGDPDRGEVYYTN
                     STHFAYDADLDPATRVFEEGRFHPMINAGAITHVWMGERRPDPGALASFVKKVFEGTE
                     NAQIAFSPEFTICNDCSRVDRGLLDACPVCGSSDVDGITRITGYFTRTSSWNPGKRGE
                     LRDRK"
BASE COUNT         1623 a         1965 c         2364 g         1459 t
ORIGIN      
        1 tcgggacctt cgtaagtgtc tcccagtgcc gggattgcaa cggctccggg aagaagatcg
       61 attcccgttg cgccaactgc ggcgggtccg gcagggtcag gaccaggcac accgttgagg
      121 tcaggatacc gcctggagtg gattctggga ccagactccg tgtagcggga gagggcatgg
      181 agggatccca ggggggtcct ccgggagatc ttttcatctc aataaaggtc gaaagtcacc
      241 ccctattcga gagagacagg gatgacctgc atgccaggct tgatctgaag tatcctcagg
      301 tcgtgctcgg agcttccgtc gaggtcccga ctcctgaagg gacagacagg atcgagatcc
      361 caccggggac ccagggaggc aagaccttca gggtaaaggg taagggcatg ccccgcctga
      421 ggggttcagg aaggggagac ctttacgccc atgtttttat cgaagtcccc agggacctca
      481 gcgagaagga gaggaccctt gtagaagccc tggcccagga gatgaatgtt ccgatccagt
      541 cgacaggcct catggatcgg atccggcagc tcttctcgtg atgttataaa agggatgaca
      601 tgctagacta tcaatggacg ggaggcgggc gagcctccct tttctattag agtcgaaaaa
      661 ccggaggtgt tcaaagtcat gagcgtcgag gggttttggt ggtatataac gctcgaagga
      721 caggacggta acgaagagga tctgtccgcc ctggccgatc tctcggggag tatcggctcg
      781 gagataaagg aactgccggg caggattgcg cttaaagcct atttccgtag caccagggac
      841 ctggggttct ggatcggaag ggtccaggac gtaatttcac cctggccggg tgtgaggatc
      901 gttgatatgg ggcggataga gaaccgccaa tggcacacgg catggaaaga tgctttcccc
      961 cccctgggtg tgggcagcag gttcgtagtg atggcaccat ggcataaggg gaaggagcct
     1021 gaagggagaa cacctcttta catatatcca ggaagtgctt tcgggacagg ctaccacgag
     1081 agtacccaga tcgcccttga gctgctggag catacggtca aaccgggcat gaaggtcatc
     1141 gatgttggta ccggatcggg gatcctggcc gtggcctccc tcaggatggg ggcatccggc
     1201 gtcattgcca ctgacaacga tccggccatt atccccgaga tgagggagaa cctgcgcctt
     1261 aacgacatac ctgacgaggc ggtggatatc cgtacgggag acctccttga agggatccag
     1321 gggaaggtgg accttgtctg cgcgaatatc gttttcgatc ccctggtgtc gatgataccg
     1381 gttataccgg cacttctgac agagggaggc ctggctgtct tctccggtct gacgctgaag
     1441 gaaagggaca gattcctgga ggtgttgccc tcgggcggtc tcgcaccgac aagggagatc
     1501 aggaaggggg agtggtgggg tgtcgcttcc tcgtgtaagg ctggagtcat cggtacggga
     1561 gagtgacacg acgtggatcc tcgatccccg agagggtcat cacctcctct cggtaaggcg
     1621 ttgccgagac ggggacctgt tcgaggggct tcttcccgga aggaagatcc tgatgagact
     1681 ggacctttcc tcgggagagg caagaggagt agttatctcg gaaagccctg agggctcggg
     1741 aagagagatc tggttactgg cggggcttct gaaaggcgat ccctttgaca ggctcgtggc
     1801 acagtccgtc gaaaccggtg tctcggtcct ggtacccctt ttgtgttcaa gatcagtggt
     1861 gagcatccct ccgggaaaga tcacgtccag gatggagcga tggagaagga tagccatgga
     1921 ggccaccaaa cagtgtggtc gcgcggatcc gccggagatc ctggagccgg tcccgctatc
     1981 atccatcccc tatcttcaac ttccttccca caggtttgtc accgtcacag gtccagccca
     2041 ggccatactg gcgtcccgcc cccattccag ggcggctgta gccgtgggcc ctgaagggga
     2101 ctggaccgaa gaagagctgg taatactgga gagagagggt ttctgcaggg tcagccttgg
     2161 ccccggggtg atgaggtccc acaccgccgg gatcgttgca gtggctgtac tctccatgat
     2221 gttggacgga gatgtccatg tatgaccgtc tcaggggggt gagggtacgg atccttccca
     2281 tgggctgtcg caccaacctg ttcgaatctg aagccctggg ctgctccttc acggaggctg
     2341 gtgccctcct cacggatgag gaagtgttcg atgcagctgt tgtcgttacc tgttccgtta
     2401 cctccgaggc ggacaggaag tgcaggcaga acctcaggag gctcagacgg gagaaccccg
     2461 acgggatcat cattgccgcc gggtgctggg cccagaggat ctctccggag gaggcgcggg
     2521 acctgggggt cgactgcctg gtagggaaca ggctcaagtt caggatccca gggatagtga
     2581 cagagatgat agaacatggg agacccacgg agggaccacg catcgaaagg gtcgatgtgc
     2641 agaatctcaa ggagtgggac cctctttatc tttctgcacg caggaccagg accagggcat
     2701 tcctaaagat ccaggacggt tgcgatcatc gatgtgccta ctgcatcatc ccggacctga
     2761 gggggatgcc cgttgagaga caccccgggg agatcctcca ggaggtgagg atgcttgcat
     2821 tgtcggggtg cagggaagtc gtcttcactg ggatcaatct gggtatgttc ggcaggacct
     2881 caggagtctc cctgggagat atcgtcaggc gcaccgggca agtggaaggg atcgagagag
     2941 tccggttcgg gtccctcgag cctttctgcc tgaccgagga tcttcttgag gctctggctg
     3001 atactcctca attctgtccc caccttcaca ttccgctcca gagcggcgat ccggtcatcc
     3061 tggaaaaaat gggccgcggc tacacccccg gagagttcga ggctcttgtt ggaagggcga
     3121 ggtatttcct tggagaagac cttcacctga gcaccgatat aatcgtgggt ttcccggggg
     3181 agaccgacaa ggctttcctc tccactctgg agatgctcga gagagcaggt gccggaaagg
     3241 tccatgtatt tcccttttcc ctgcgggagg gaacaccagc ctcacttatc gatggtcgtc
     3301 tccccccccg ggtggtacgc tcaaggatgg atcaggcact ttcaatggct tcagaactac
     3361 tggaccgtta tgcaggccga tgggtggggg aggaagtgga gatcctggtg gagaagagcc
     3421 aggggggagt ggtccagggt ctctccagtc actttctgcg tgtcagggtc ccaggagagg
     3481 ctctccaggg agagatcacc acggttgtcc ccggagatac gaaaaaaggg gtccttctcg
     3541 ggatggtgtg atataattcc ctaccagata ttgccgtgtc cctttcgcaa agggggtcgg
     3601 aagccccctg ggcccggccg tccagcatcg aggtggcggg tgttctttta gcggggagct
     3661 gatcatgtga gccgcgactg tcttttctgc aggatcgccg aaggaagcga aagggcggat
     3721 atcgtttaca gcgatcccga tgttatagta atacgcgata tccaccccag agcaccggta
     3781 catcttctgg tgatacccag gcagcatgtg ggatccgccg atgaggtcga agatccttcg
     3841 gtatggtcct cgttaatgaa tgcggcggtc agggtcgccc gcgacatggg cctttgcgca
     3901 ggcgggtatc gtctcgtggt caactgtggg gcaggagccg gacagaccgt gttccatctt
     3961 catgtccacc tgctcgccgg acgggtgttc cagtggccgc cgggataacc ctgagtgaga
     4021 gagaaggggg ggaacgcaaa tgacacaggt cgttcgacgc gataacgaat ccctcgaaga
     4081 tgctctcaag cgcttcaagc gagaggtatc caagggaggc atcctgcgcg aggcgcgcag
     4141 gcgcaagcat tacgagaaac ccagcgaggc gaagaaaagg aagagagagg acgcttcgag
     4201 aaaacgcagg gggaagtagc aggcatatga ccgggctgga ggaaaggatc cacctcgaga
     4261 tggtgaaggc catgaaagcg agggaccaga gctccctcgt accactgaga atgctcaagg
     4321 cggccctgga taacacgagg gtcgagaagg gccggagcgg gggatccctc tcagacgagg
     4381 acatcctgtc tgttgtccgg agactcgtga aacagcgcaa ggacgccgcc gagcagttct
     4441 cggccgtcgg ggcacttgga cgtgcagaag aagagaggca ggaggcggat cttctctcct
     4501 ctttcctccc accctccatg gacgacggga ctatcctcga cgtggtccga tccgttgcgg
     4561 ccgagacggg ggctaaagga ccctcggacc tgggaagggt catgggcaga acgatggccc
     4621 aactcaaggg caaggccgag ggtgaaagga tacgcggtat tgccgccaga ttcctggata
     4681 ccctctgaac acatggatat cttttagaga atagaaaggg ccccgcctaa ggcgaggccc
     4741 ttttttacgg gttgacactc aaaatatagt ggtataatgt tgaaaacatc ctagatatag
     4801 ggagtccgat gccatgcgat gtcccaggtg cggcctgatg gagaccaggg tcctcgagac
     4861 aaggaccgcc agcgagggaa gggtgatccg caggcgcaga gagtgcccag gatgcatgaa
     4921 ccgcttcacc acctacgaaa ggctcgagga tcgtcccgtg ctgtggatcg tcaagaaaag
     4981 cggacagaga gaggcttttg acagggagaa gcttctcaag ggttttgcca aggcctgcga
     5041 aaagcttccc gtcccgctgg agcagataga ggaggctgta tcccggatcg aggagaccct
     5101 tagagagaca ggccagggcg aggtctcgag catgaccgta ggttcccttg ccatgatgga
     5161 acttcgggat atccacaagg tcgcctacgt gaggttcgca tcggtctaca gggaattcac
     5221 ggacgtatcc agtttcgtag aggagatatc aagactccgc gatgaaagga atggtcaaag
     5281 gtgaagactc ccgaaagcgt tgcccatgtc gaaggtaaca gccaggggga attgtcagcg
     5341 ggaaggcaga tgctcatctt ttccggcagg ggagagacca ctgacctggc cctcatggtc
     5401 gaggctcttg cccaggagga ccgttcaccg tggaacgctt accgcatacg cgatgccctg
     5461 atcagggagg caggggtcga cccggccacc gcggaggaga tcgcacttga ggttgaggca
     5521 gatatcctcc ggcacggtaa ggagagggtc acgacctcaa ctatacggga actggtcaac
     5581 gtcaagcttt tcttccgcgg tctcgatgct aaactcgcgg accattcgcg gctcggtctg
     5641 cctgtctatg acctggaatc gatgatgctg agccctaacc gggagaacag caacacatcc
     5701 cacaacccgg agtcgatcaa cctctccatc gcggagatga tcctcaagga gtacgccctc
     5761 aagaaggtct tcccgggcga ggtggcccgc gcccaccttg cgggggatat acatcttcac
     5821 gatctcggga tggtgaacag accctactgt tcaggacaga gcatggccta cgtcatcagg
     5881 tacggacttg acctgccctc catcacaagt gtctctgccc ctgcaaggca ccccgatgtt
     5941 cttctcgccc atattctcaa gatgacatcg gtgctgcaga acaactttgc aggagccatc
     6001 ggctgggatg cggtgaacgt attctttgcc ccctttctga cggggcttcc ctacgaaaag
     6061 gtcaggcagc ttgcccagat gctgatcttc gagttcaacc agctcgccgg gggaaggggg
     6121 gggcaggttg cctttaccga tatcaacctc tactacgaga tacccgatca tttcagggaa
     6181 gtacaggcgc tgggacccgg cgggttgccg acgggcagga cctacggcca gtactccgag
     6241 gaatcgaaac ttttcctgag agcccttttc gaggtctacc tggagggtga cagccgcgga
     6301 cagccctttt tcttccccaa gccccttctc cacataactg accgcttctt ccaggagcca
     6361 gggtgggaag aattccttcg ttttgcctgc ctggtagcat cggagaaggg gagtacctat
     6421 ttcgttttcg accggggggg tgtggcaaag ctgagcgagt gctgcaggct cagcttcgaa
     6481 ctctccgagg atgacctgag agaggctgcc acgccctgga agatgcgtta ctgtgccctg
     6541 cagaacgtca cgataaacct tccaaggatc gcctatcggg ccggtggtga cagggatagc
     6601 ctcttcgaga tcctggacag gatgatggag gccgccgccc ttgcccatgt caggaagagg
     6661 gagttcctct cggagatgct tgccttgggt tccaaagggc ccctggcggt cctctgtgtg
     6721 gatcacgacg gcgagaccta tatcaggctg aaaaagagca gctatctaat agggctcatc
     6781 gggctggaag agatggtcca ctgcgttaca gggaagagaa tgcacgaaga ccgcgaggcc
     6841 atggatctcg gacttgccgt gaccaggtac atggacctga agtgccggga gctgacggaa
     6901 cgactgggcc tcaagatcgt tctggagcag accccggcgg agagtactgc ataccggctt
     6961 gcccggctgg acctcaagtt cttcccggac caggcctcaa tgttcgtcaa gggagacccc
     7021 gaccgggggg aggtctacta taccaacagc acccacttcg cctatgatgc cgatctcgac
     7081 cccgcaacca gggtcttcga ggaggggcgt ttccacccca tgataaacgc cggggccata
     7141 acccatgtct ggatgggaga gcgcaggccc gatcccgggg cgcttgcctc tttcgtcaaa
     7201 aaggtcttcg agggaaccga gaatgcccag atcgccttca gtccggaatt caccatatgc
     7261 aacgactgca gccgggtgga caggggactt ctcgatgcct gtccggtttg cggcagtagt
     7321 gatgtggacg ggatcacccg tattaccggg tatttcacca ggacatcatc ctggaacccc
     7381 ggaaagaggg gcgagcttcg ggacaggaag a
//
LOCUS       DAOPKQ010000049         7321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10819_length_7321_cov_5.441096, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000049 DAOPKQ010000000
VERSION     DAOPKQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10819_length_7321_cov_5.441096"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PK146_02800"
     CDS             complement(<1..979)
                     /locus_tag="PK146_02800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPE91200.1"
                     /translation="MEQTATMEITEQERKTFGGFGLRKELLKALDDRGFIEPTPVQEA
                     VLGQDNLNADLIVRARTGSGKTLAFLLPLMSDDNLDPSRPGVVVISPTRELALQISRE
                     AAWVSRGINCPCTSLVGGMDMSSQIRDLRRGVALVIGTPGRILDHVRRGTLCMDSVHT
                     VVLDEGDHMLDMGFRDELEGILDGASGRKRTWLFSATMPPAVRALSRKYLSDPRMISL
                     VDEGAQHEDIIHTVYTTPKNRQMDGLVNVLLWENSSKGLVFCHTRAETIEVANRLTQE
                     KFSSCCLHGDMSQRERNHALSMFVSGRVSHLVATNVAARGLDVPDVEHVF"
     gene            complement(1166..2617)
                     /locus_tag="PK146_02805"
     CDS             complement(1166..2617)
                     /locus_tag="PK146_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE91201.1"
                     /translation="MSRNKNDERLAGFLQRIFDRIPVGLYRTTPEGRIIHANLALARM
                     LGYESVEDLKSRDLEIEGYEPSYPRKAFKERIEREGAIHGLDARWCRTDGTFIDVMEY
                     AEAVRDGSGKVIFYDGAVEDVTEFKNAEKTIMEREREYRSLSGMFRLLADNMQDMLWA
                     KDLQGRYLFANRAFCERLLNASDTSEPIGKTDMYFAERERRAHPEDPEWHTFGEICVG
                     SDAETIRVKGPCQFEEFGNVQGQSLFLDVRKAPLVDEGGDIIGVVGSARVVTREKELE
                     SEQRRSQKELEEACQRLRSTQESTLRVMAKLVETRDPYTSGHQGRVALLAVAIAREMG
                     LDEETMEGLRVASLVHDIGKLRVPIETLSKPGYLATMEMELIKEHSRAGAEIISEIRF
                     FRPIDEIILQHHERLDGSGYPRGLSGEQILIEARILAVADVVEAMSADRPYRPALGMD
                     VALEEIRSCSGRLFDPEVVRVCCQLFESGRFGF"
     gene            complement(2788..3669)
                     /locus_tag="PK146_02810"
     CDS             complement(2788..3669)
                     /locus_tag="PK146_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE91202.1"
                     /translation="MDIVNAAGDSWYIPGPTNLGVCVRDGRALLIDSGNDESAGRKVL
                     QALKEKGWIPEAIINTHSNADHIGGNAFIQKRTGCRIASTRAEAALIEDPWLEPALLW
                     GASAFGDISGKFLRAQPSKVTDVIPSSGTVLDTDLEAVPLPGHFLDMVGIRTPEGVFF
                     IADSLFSASILDKYGMVVCFDVAAQMRTLEYLGEVEAALFIPSHAEPCTKIGTLAKRN
                     RDALELVRDRILEVCKAPASREDILADLAGLFNINLNPSQYILTHVTVSAHLSCLADQ
                     GVLRPLFEKGRMLWTLQ"
     gene            3751..4632
                     /locus_tag="PK146_02815"
     CDS             3751..4632
                     /locus_tag="PK146_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HPE91203.1"
                     /translation="MTDLILVDGHALAYRAYFGVKNPMRSPENVPVNGVFGFGRMLMG
                     IMEGSRAKEGAVVFDSPGPNFRKDLYPPYKANRPIPPEDFIVQLPLMMDLTRSLGIKV
                     LSDSAFEADDMIAAAAVREAERGKGVVIVTPDKDLFQTLSEGDITIMRPAGRDTFTRM
                     DASLFRDSFGFEPCHMAEYQALMGDRTDNIPGVPGIGPVTARTLVKCFGDLESLYGSI
                     SSVKETLRGRLERDRDSAFLSRELARLRLDAPVPEDLFLKEPDREELVCFCERNGLEV
                     LTGTCSSFCTSVATGWR"
     gene            4636..6474
                     /locus_tag="PK146_02820"
     CDS             4636..6474
                     /locus_tag="PK146_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPE91204.1"
                     /translation="MTRDSGGFLPVNRTDMERRGWSEVDFVFISGDAYVDHPSFANSV
                     IPRVLEGRGYRVGVIAQPDWRRLDDLLVLGRPRLGVLVSAGNLDSMLSHFTAAKKPRS
                     DDPYSPGGRAGTRPRRATVVYCNRAREAWKDIPLIIGGVEASLRRFAHYDYWSDSVRR
                     SILVDSKADLLVFGMGEKPILEITSALSQGVPVNQIRGVKGTCFRSKGGDLPADAIIL
                     PSFEETVRDRTAFAEAFRLQYLEQDPFRGRPVAQDQGAWYVVQNPPSRPLSTEEMDWI
                     YDLPYIRAPHPMYDERGGVPAIEEVRFSITAHRGCFGSCSFCSISSHQGRIIQARSDG
                     SILREARILTGLPGFKGYIHDVGGPTANFHTPSCAAQLSRGACSDRECLWPGPCPGLE
                     TDQSGYFSLLKKIRSLSGVKKVFVRSGIRYDHLLTARDPGLVRELCEHHVSGQLKVAP
                     EHVSPRVLRLMRKPGKEVFLEFKRQFDMANRDLGKEQYLVPYLMSSHPGSTIADAVEL
                     AQFLKEIRYHPEQVQDFIPTPGTLSTCMFYTGIDPLSGEEVYVPRSAHERKLQRALLQ
                     YRSPANWKLVQEALRAAGRQDLIGTRPGCLVPPASQGRARGKGRQN"
     gene            complement(6490..6936)
                     /locus_tag="PK146_02825"
     CDS             complement(6490..6936)
                     /locus_tag="PK146_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPE91205.1"
                     /translation="MRKALVAIDGSDITKPLVRFAFERARKEKLEGLDFIYVFQPYPA
                     ALFPGMPVPVTLEEGHAEATRKALEALVQKERKETGAEKVEVSMIFPYGSPSGEIIRQ
                     AEEFPYRSIFIGHRGMGSLERFFIGSVAARVVEHAPCTVIVFRPRD"
     gene            complement(6971..>7321)
                     /locus_tag="PK146_02830"
     CDS             complement(6971..>7321)
                     /locus_tag="PK146_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPE91206.1"
                     /translation="VEFALIRTHPEIGYEIMKNIELPWNLADMILQHHERMDGSGYPA
                     GLKGEEILLESRILAVADVVEAMSSHRPYRASLGIGSALKEIEENRKKKYDADVVDVC
                     LSLFREKGFKFPGA"
BASE COUNT         1479 a         2231 c         2048 g         1563 t
ORIGIN      
        1 ggaagacgtg ctccacgtcg ggcacatcaa gaccccttgc cgccacattg gtcgccacaa
       61 ggtgggacac cctgcccgag acgaacatcg agagggcatg atttctctcc ctctggctca
      121 tatccccgtg gaggcagcag gagctgaatt tctcctgggt aagccggttg gccacctcga
      181 tcgtctcggc cctggtgtgg cagaatacaa gaccttttga ggaattctcc catagcagta
      241 cgttcacgag cccgtccatc tgccggttct tcggggtcgt atagaccgta tggatgatgt
      301 cctcgtgctg ggcgccctcg tccacaagcg agatcattct tgggtccgaa aggtatttgc
      361 gggaaagggc ccggactgct ggaggcattg tggccgaaaa gagccacgtc ctcttccttc
      421 cggaagcgcc gtcaagtatg ccttcaagct cgtcgcggaa acccatgtca agcatgtggt
      481 cgccctcgtc aaggactacg gtgtgaaccg agtccataca gagagtgccc cttcggacat
      541 ggtccaggat ccttcccgga gtccctatga caagggcgac gcctctcctg agatccctga
      601 tctggctgga catgtccatg ccacccacaa gggatgtaca gggacagttg atccccctgg
      661 atacccatgc ggcttcacgg gagatctgca gcgccagctc ccgggtggga gatatcacaa
      721 ctacgccagg tcttgatggg tccaggttgt catcgctcat aagcgggaga aggaaggcaa
      781 gggtctttcc agagccagtc cttgccctca cgataaggtc ggcattgagg ttatcctggc
      841 ccagaacagc ttcctggacc ggggtcggtt cgatgaatcc cctgtcatca agggccttga
      901 gcaattcctt ccggaggccg aaacctccaa aggtctttct ttcctgttcc gttatttcca
      961 ttgttgcggt ctgttccata aaagcaaatt cctcctgtat ttgggaaagg acgcaaaaaa
     1021 agcggcgccc accatggggc gccacctcgt gtaatccctt cacggcaatg cgactattat
     1081 cgttatcggc tgtcaaatgt caagcaggaa ccaaagcatg cactgcgtga acagtcaact
     1141 gttcacctgt caccgtttac cggtttcaaa atccaaatct tccgctttcg aagagctgac
     1201 aacataccct gaccacctcc gggtcgaaga ggcggccgct gcaggacctg atctcctcca
     1261 gcgcgacatc catgcccaaa gctgggcggt acggcctgtc tgcggacatg gcctccacga
     1321 catcggccac ggcaaggatc ctggcctcaa tgaggatctg ttcacctgaa aggcccctgg
     1381 ggtaacccga accgtcaagg cgttcgtggt gttgaaggat gatctcgtcg atgggacgga
     1441 agaacctgat ctccgaaatg atctccgccc cagccctgga gtgttccttg atgagctcca
     1501 tctccatcgt ggccaggtag cctggtttgc tcagggtctc gatgggaact ctcagcttgc
     1561 cgatatcgtg gacgagagag gccactcgga ggccctccat ggtctcttcg tcgagcccca
     1621 tctcccttgc gatcgctacg gccaggagcg ccactctccc ctggtgtccg gaggtgtagg
     1681 ggtctcgggt ctccaccagc ttggccatca ccctgagggt actctcctgg gtcgacctga
     1741 gcctttggca ggcctcttcc agctccttct gggaacgtct ctgttctgat tcgagctcct
     1801 tctcccgtgt cactaccctt gcgcttccca caacgccaat gatgtccccg ccttcgtcaa
     1861 ccagaggcgc ctttcgcaca tcgaggaaga gcgactgtcc ctggacattg ccgaactctt
     1921 cgaactggca cggccctttg acccttatcg tctcggcatc ggaccctacg cagatctcgc
     1981 cgaaggtgtg ccactcgggg tcctcggggt gtgcccttct ttccctctct gcgaagtaca
     2041 tgtccgtctt tcctataggc tcgctggtgt cggaagcgtt caggagcctc tcgcagaaag
     2101 cccggttggc gaacaggtac cggccctgga gatccttggc ccagagcatg tcctgcatgt
     2161 tatcagccag gagccgaaac attcccgaga gggagcgata ctccctctcc ctctccatga
     2221 tggttttctc ggcatttttg aactccgtca cgtcctcgac ggcaccgtcg tagaagatca
     2281 cctttccgga cccgtccctg actgcctctg cgtactccat cacatcaata aaggtcccgt
     2341 cggtcctgca ccatctggcg tcaagcccgt gaatggcccc ctctctctca atgcgctcct
     2401 tgaaggcctt cctggggtag gagggttcgt agccctcgat ctcgagatcc ctgctcttga
     2461 gatcctccac gctctcatag ccaagcatcc ttgccagtgc aaggttggcg tggatgatcc
     2521 tcccctccgg ggtcgtcctg tagagcccca cgggtatgcg gtcgaagatc ctctgtaaaa
     2581 acccggccag tctttcatcg ttcttatttc ggctcaaatt ggccataaat gactcctgat
     2641 aaagttatcc gcaggcaacc gttggcggtc tcacccggtt tttgtgagga acggcaccag
     2701 tcggcttttt gcggtttgag accatgtgac tccgcgtaca atctatcata aaagaagact
     2761 tctccaaaac agtcccttcc gcaaatccta ctggagggtc cagagcattc tccccttttc
     2821 gaagagaggc ctgagcaccc cctggtctgc gagacaagag aggtgggccg ataccgttac
     2881 atgggtaagt atgtactggg aagggttcag gttaatgttg aaaaggccgg ccaggtcggc
     2941 aagaatgtcc tcccttgatg caggggcctt acagacttcc aggatcctgt cgcgtacgag
     3001 ttcaagggca tctctgttcc tcttcgcaag agtgcctatc tttgtgcagg gttctgcgtg
     3061 gctgggaatg aaaagggccg cctcgacctc tcccaggtac tccagggtcc tcatctgcgc
     3121 cgccacatcg aaacacacga ccattccgta cttgtccagg atagaggccg agaaaaggct
     3181 gtctgctatg aagaaaaccc cttcgggtgt cctaatgcca accatgtcaa ggaaatgacc
     3241 cgggagcgga acggcttcaa gatccgtgtc aagaaccgtc cctgaagaag gtatcacgtc
     3301 ggtgaccttc gaaggctggg ccctgagaaa cttcccgctg atgtcaccga aggcagatgc
     3361 cccccagagc agggccggtt cgagccaggg gtcctctata agggccgcct cggcccgtgt
     3421 agatgctatc ctgcagcctg tcctcttctg gatgaaggcg ttcccgccta tgtgatcggc
     3481 gttggagtgg gtgttgatta ttgcctcagg gatccacccc ttttccttta gggcctgaag
     3541 taccttcctc cccgccgatt cgtcgttgcc gctgtctata aggagcgccc taccatccct
     3601 gacgcagacc cccaggttgg tgggaccagg gatgtaccag ctgtctcccg cggcattcac
     3661 tatatccatt tgttgtaccc cctcccttgc tgataccata cgactgcaag gagaggatga
     3721 caaccgcctc cttgccggaa ggagaaccca ttgaccgacc taatcctcgt agacggccac
     3781 gccctggcct acagggccta tttcggcgtc aagaacccca tgcgatcccc tgaaaatgtt
     3841 ccggtgaacg gggtgttcgg cttcggcaga atgctcatgg ggatcatgga aggctcccgg
     3901 gcaaaagagg gagcagtggt cttcgactca ccggggccga atttccggaa ggatctctac
     3961 ccgccctaca aggcaaaccg gccaatcccg ccggaggact tcatagtcca gctaccgcta
     4021 atgatggacc tcacccgctc tctggggatc aaggtcctct ccgacagtgc ctttgaagcc
     4081 gatgatatga tagccgcagc tgccgtaagg gaggccgagc gaggcaaggg tgtggtcatc
     4141 gtgacccccg acaaggacct gttccagacg ctgagcgaag gcgacataac aatcatgcgg
     4201 ccggcgggca gggatacctt cacccggatg gacgcctccc tcttcaggga ctccttcggc
     4261 tttgaaccct gccatatggc agagtaccag gccctgatgg gagacaggac ggacaacatt
     4321 cccggggttc cagggatcgg cccggtcacc gcaagaaccc tggtgaaatg tttcggagac
     4381 ctggaatctc tttacgggag tatcagttcc gtgaaggaga ccctccgggg acgactggaa
     4441 agggatcggg actcggcctt cctgagcagg gaactggctc gtctccgact ggacgcgccc
     4501 gttccggagg accttttcct gaaagagccc gacagggaag agcttgtatg cttctgtgag
     4561 agaaacggcc ttgaagtcct gacgggtact tgcagctcct tctgcacatc tgtggcaaca
     4621 gggtggcgat agccaatgac aagggactct gggggttttc tccctgttaa caggacggat
     4681 atggagcgcc gcggctggag cgaggtagac ttcgtcttta tctcaggaga tgcctacgtg
     4741 gaccatccaa gttttgcgaa ctccgtcata ccgagggtcc tcgaaggacg gggttaccgt
     4801 gtcggtgtga tcgcccagcc cgactggagg agactggatg atcttcttgt cctggggcgc
     4861 ccccggttgg gtgttttggt ctcggcgggc aaccttgact caatgctctc tcacttcacg
     4921 gcggcgaaaa aaccccgtag cgatgacccc tactccccgg gtggcagggc cgggaccagg
     4981 ccccggagag ccacggtggt ttactgcaac cgggcaaggg aggcctggaa ggacatcccg
     5041 ctgatcatcg gtggcgttga ggcgagcctg cgaaggttcg cacactacga ctactggtcc
     5101 gacagcgtca ggaggtccat cctggtggac agcaaggccg acctcctcgt cttcggcatg
     5161 ggggagaagc cgatccttga gatcacgtct gctctctccc aaggggtgcc ggtgaatcag
     5221 atcagggggg taaagggtac atgcttccgg tcgaaaggag gagaccttcc cgccgacgca
     5281 ataatcctac cctctttcga ggagaccgtc cgcgacagga cggccttcgc cgaggctttc
     5341 aggctccagt acctggaaca ggaccccttc agaggaagac ccgtagccca ggaccaggga
     5401 gcctggtacg tggttcagaa ccctccctca aggcctcttt cgacggagga gatggactgg
     5461 atatacgatc tgccatacat aagggcgccc caccccatgt acgacgagag agggggcgtt
     5521 cccgccatcg aggaggtccg gttcagcata acggcccaca gaggctgctt cggaagctgc
     5581 tccttctgtt ccatatcctc ccaccagggg aggatcatcc aggccaggag cgacgggtcc
     5641 atcctcaggg aggcaaggat cctaaccggc ctgcccggtt tcaaggggta catccatgac
     5701 gtaggcggac cgacggcgaa cttccacacc ccctcctgcg cggctcagct ctccagggga
     5761 gcctgctccg acagggaatg tctctggccg gggccgtgtc cgggactgga gactgaccag
     5821 tcggggtact tctcactcct taaaaagatc cgttcccttt ccggtgtcaa gaaagtcttc
     5881 gtccgctcgg gtatccgtta cgatcatctc cttacagccc gtgaccccgg gcttgtcagg
     5941 gagctctgcg aacaccatgt cagcggccag ctcaaggtgg cccctgaaca cgtatctccc
     6001 cgggttctgc gtttgatgag aaaaccgggg aaggaggtct ttctggagtt caaaaggcag
     6061 ttcgacatgg ctaacagaga tctgggaaag gagcagtacc tcgttcccta tttgatgtcc
     6121 agccacccgg gctccaccat cgccgatgcc gtggagcttg cgcagttcct gaaagagata
     6181 cggtaccacc cggagcaggt ccaggacttc atccccacac cggggaccct ttcaacctgc
     6241 atgttctaca ccggaatcga tcccctttcc ggtgaagagg tttacgttcc aaggtccgcc
     6301 cacgagagaa agctccagag agcgcttctc cagtaccggt ccccggcaaa ctggaaactc
     6361 gtccaggagg ccctcagggc agctggcaga caggacctga taggcacccg gcccggctgc
     6421 ctggtcccgc ctgcttcgca aggaagggcc aggggcaagg gacgccagaa ttaaagggaa
     6481 cttgctggtt cagtccctgg ggcggaaaac gatgaccgtg cagggggcgt gctccaccac
     6541 cctggcggcc acactgccaa taaagaaccg ctccaggctc cccattcccc tgtgaccgat
     6601 gaagatagac ctgtagggaa actcctcggc ctgacgaatt atctcccccg aggggctacc
     6661 gtaggggaag atcattgaca cctccacctt ctcggcacct gtctccttgc gttccttttg
     6721 gaccagggcc tccagggcct tcctggtcgc ttcggcgtgt ccctcctcaa gggtcaccgg
     6781 gactggcata ccggggaaga gcgccgcggg gtatggctgg aacacgtaga taaagtcaag
     6841 cccctccagc ttctccttcc tggcccgctc gaaggcgaac ctgaccagcg gtttcgtaat
     6901 gtcgcttcca tcgatcgcca caagtgcctt ccgcatcctt tatcacctcc atgatcctgg
     6961 tgatccctga tcaggcacca gggaacttga atcccttctc cctgaaaaga ctgaggcaga
     7021 cgtcgactac gtcggcgtca tactttttct tcctgttctc ctcgatctcc ttgagggcgg
     7081 acccgatccc cagcgacgcc ctgtaggggc ggtgagagga catggcctcc accacgtcgg
     7141 ccacggccag gatccttgac tcgaggagta tctcctcccc cttcagaccg gcggggtaac
     7201 ctgaaccgtc cattctttcg tggtgctgga ggatcatgtc tgccaggttc catggcagtt
     7261 cgatgttctt cattatctcg tagccgatct cgggatgggt cctgataaga gcgaactcga
     7321 c
//
LOCUS       DAOPKQ010000050         7320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10823_length_7320_cov_4.463042, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000050 DAOPKQ010000000
VERSION     DAOPKQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10823_length_7320_cov_4.463042"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PK146_02835"
     CDS             <1..411
                     /locus_tag="PK146_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE91207.1"
                     /translation="LNRGADDYLVKPFDFRELVARINAVTRRRAELKRPILNCGPLVL
                     DPIARECRAEGETVPLRRREFDILELLMRYENQVFTRERIIAHVWQKEYDGTSNVVDV
                     HVKYLRDKLRPYNLDTVVVTVRGVGYKVTCPDYE"
     gene            456..1934
                     /locus_tag="PK146_02840"
     CDS             456..1934
                     /locus_tag="PK146_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPE91208.1"
                     /translation="MARLSDGRWPLRLRSFRFRLVFFSALTIMVTIAIFGMVVFTLFT
                     SLEINRIDRTLSVCLEEVISFRQKAGRFPIQVWEGRSRGVVLQVYDQDMSLLFHLPPG
                     LSAPMIPGDLLEAASGRKHPFTFDPAKSRYLPRLWWVLPWNYLSHRNLWRTCTTVALL
                     GEKEVFLVSMVPMVWLLESRQLLFWMIVIAGIAGMIVSLAVGRLLAARAIRPLKDINR
                     ALSEVSISNMSIDPTPLGTDREIDEIVNHINTMLKKLDMSMKNLQQFTSDASHELRTP
                     LAVMRGAVDVALLRERDPAYYIGKLQELMYNIDDMQNLVGALLELARLDDFKGLDSRE
                     PVDLLLTAEDAIANMEAITQRRGQTIEKDLHPAPTQGRDAMVLRLAYNLLENASKHSP
                     PGSKIGVSTYIDQERDHAVMEIRDDGPGLDPEEINQCFDRFWRADFSRTTPGFGLGLP
                     LVKRIAQIHSAFIEVESSKGGGSLFRVNFPLDSEALKDYDFE"
     gene            complement(1984..3396)
                     /locus_tag="PK146_02845"
     CDS             complement(1984..3396)
                     /locus_tag="PK146_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HPE91209.1"
                     /translation="MKRSKGSRFFLAALVFLLSAAVVLPLSSGTAAAQDVFTGNPIAK
                     IVKEASPAVVNIDTEAMVQRSFSPFPDDPFFREFFGDRFDRFSREVPMRGKGSGFIVT
                     GDGYILTNNHVVDGPDNMTIEVTLSDGRSFPARVVGKDPSYDLAVIKIDSSDLPTVPM
                     GDSDRIEVGEWVIAIGNPYGFESSVTVGVVSTKNRSVRARNLNFDGFIQTDAAINPGN
                     SGGPLLGLDGQVIGINTAIVPYAQGIGFAVPVNMAKQIMDDLIQYGKVNRGWLGVYLQ
                     PLTREFASAYGIKSGEGALVSDVVKGAPAEKAGILRGDVVTAIDGRKVKDHGDLIVAI
                     RQHLAGEKVELTVIRKGVQKKINVTLSAVPTEEVSEAPVKDEPVSSSKLGISIASGTP
                     ELREKYGIEETGGAIITGVLPGSLGHRMGLREGDVILEINGETVKDHAQLEEILSRDL
                     KSLAMLVLREGRTFYVSANL"
     gene            complement(3437..4255)
                     /locus_tag="PK146_02850"
     CDS             complement(3437..4255)
                     /locus_tag="PK146_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPE91210.1"
                     /translation="MTGEFRCVTFLSDFGLSDETVACCKGLLLRRGVTLPVIDISHEV
                     PPFDVISGGWMLAGAVLYMPVGVHLAIVDPGVGTDRKILVIRSVRGDLFVGPDNGLLV
                     PAAERTGGISSSWSIERPPAGAEQVSPTFHARDIMAPVVADLVGGVPPEKCGIEIPRG
                     GLNRFPLPAPQSSPGKISGHVTLVDAFGTIRTDIPWNLLGKLGDSEIKVSCGWLFSIP
                     VGRTFSDVSSGDMVALEDSWGFVSIAVNRGRASDMLGFKPGDPISVELTIPGRP"
     gene            complement(4368..5015)
                     /gene="jag"
                     /locus_tag="PK146_02855"
     CDS             complement(4368..5015)
                     /gene="jag"
                     /locus_tag="PK146_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPE91211.1"
                     /translation="MEELESFVVEVNSLEEARHIGSLRWSVEPSDVEIEVVDEGKRLF
                     GLLGKKMRVSISRKLGRPEASPPQGSIPFLEELLGKMDLSIEPVMTDGDNIDLAGEDT
                     GIVIGRNGETLKAVEFLTNLVLREKDPGRRIRIDSGGYKARRQDSIEKIALAAAREAQ
                     RKGRPIRLEPMTSWERRIVHMSLKERDDVSTGSVGSEPMRRVVVSPARKGSQGKA"
     gene            complement(5046..5834)
                     /locus_tag="PK146_02860"
     CDS             complement(5046..5834)
                     /locus_tag="PK146_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPE91212.1"
                     /translation="MSALWKAAGDFLLFVLNSLYTFTHSYGIAIIILTILIRILLHPL
                     SHKQLVSMQKMQKLQPRIKILQEKYANDKDALNKEIMKLYKENNVNPAAGCLPLLVQL
                     PILILLFRVLMNLDLGGATFLGISLEGSVLSTLGEAVGLVAETHGIGQVMSAIAANPA
                     GLANVGVYAANLVLLLVVGYLTWFQQKLSGSSNPQMQFMNWFMPVFLTFICLSLPGGV
                     LLYWGVSSLIGVGQQWHIKRKTEVEMQEKPVLLKEKPQKKEGRQ"
     gene            complement(5840..6124)
                     /gene="yidD"
                     /locus_tag="PK146_02865"
     CDS             complement(5840..6124)
                     /gene="yidD"
                     /locus_tag="PK146_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPE91213.1"
                     /translation="MKGLPARTAVKVIHFYQRFISPLLGDRCRFYPSCSSYAIQGIER
                     FGLARGTALAFLRIIKCGPWHSGGYDPVPDEFPSLFDRVLHQREYSPKGR"
     gene            complement(6121..6525)
                     /locus_tag="PK146_02870"
     CDS             complement(6121..6525)
                     /locus_tag="PK146_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPE91214.1"
                     /translation="MRASFVFPSQDRLKKGWQFDQVFRTGRRESGALVRLLFMASPDG
                     TTRYGVAVGKQQGCSPLRSRGKRLLRESLRRLRPWVKEGFWIVCTIKTPAIGKNARDV
                     YLDMTKVFQRAGLLGPEWPGPDWDIDRRRNQG"
     gene            complement(6526..6660)
                     /gene="rpmH"
                     /locus_tag="PK146_02875"
     CDS             complement(6526..6660)
                     /gene="rpmH"
                     /locus_tag="PK146_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPE91215.1"
                     /translation="MKQTFQPHNRKRKRKLGFLQRSSSPSGRRILQNRRNKGRKRLAV
                     "
     gene            complement(6966..>7320)
                     /locus_tag="PK146_02880"
     CDS             complement(6966..>7320)
                     /locus_tag="PK146_02880"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPE91216.1"
                     /translation="EGIRWVITMLSDRTVMGTCGFHNWKKEHFRAETGYELGKYHWGK
                     GYMKEAMSAALEYGFDGMGLNRIEAFVTEGNGRSLGLLKDLGFNTEGLLRNYEWARGK
                     FQDQWVCSILKSERA"
BASE COUNT         1549 a         2223 c         1973 g         1575 t
ORIGIN      
        1 ctcaaccggg gagccgacga ctacctggtc aaacccttcg atttccgtga actggtcgcc
       61 aggatcaacg cggtcacacg caggagagcg gagctgaaga ggcccattct caactgcgga
      121 ccgctggttc tggaccccat tgccagggag tgcagggccg aaggcgagac agtgcctctc
      181 aggagaaggg agttcgacat cctggagctt cttatgcgct acgagaacca ggtcttcacc
      241 agggagagga tcattgcaca cgtatggcag aaggagtacg acgggacaag caacgtggtc
      301 gacgttcatg taaaatacct cagggacaag ctgcgtccct acaacctcga cacggtggtc
      361 gttaccgtca gaggggtcgg atacaaggtg acctgccccg actacgagta gccctccggc
      421 accttgagac cacgacctgg gagatgttga aatagatggc aaggttatcc gacgggcggt
      481 ggccgttacg gctccgctcg tttcgtttca ggctggtttt cttttccgcc ctgaccatca
      541 tggtgaccat cgcgatcttc gggatggtcg tctttactct tttcacctct cttgagatca
      601 acaggatcga ccggaccctg tcggtttgcc ttgaggaggt gatctccttc cggcagaagg
      661 cgggaagatt ccctatccag gtatgggagg gcaggtcccg gggtgtggta ttacaggtct
      721 acgaccaaga catgtcactt cttttccacc tccctccagg cctttcggcc ccgatgatcc
      781 cgggggatct tctggaggcg gcatccggac gtaagcaccc cttcaccttc gacccggcaa
      841 aaagcagata tcttcccagg ctctggtggg tcctcccatg gaactatctt tctcaccgaa
      901 acctctggag gacctgcaca accgttgcgc ttcttggtga gaaagaggtc ttccttgtat
      961 cgatggtccc gatggtatgg ctcctcgaat cgaggcagct gctcttctgg atgatcgtca
     1021 tcgccgggat cgcaggcatg atagtctccc ttgctgtggg aaggctgctg gcggcccggg
     1081 ccatcaggcc gctcaaggac ataaacaggg ccctttcgga agtctccatc agcaacatgt
     1141 cgatcgaccc taccccattg ggcacagaca gggagatcga cgagatcgtc aaccacatca
     1201 acaccatgct taaaaaactg gacatgagca tgaagaacct gcagcagttc acctccgatg
     1261 cgagtcacga actgaggaca cccctggcag tgatgagggg cgccgtcgac gtagcactcc
     1321 tcagggagag ggacccggcc tactacatcg gcaaactcca ggagctcatg tacaacatcg
     1381 acgacatgca gaacctggtg ggagccctcc tggaactggc caggctggac gacttcaagg
     1441 ggctggattc gagagaaccg gtggatcttc tcctgaccgc cgaggatgcc atcgccaaca
     1501 tggaggccat aactcagagg agaggtcaga ccatcgagaa ggacctccac cctgccccga
     1561 cccagggacg ggacgccatg gtcctgaggc tcgcctacaa cctccttgag aatgccagca
     1621 agcactcccc tccgggcagc aaaataggtg tatcaaccta tatcgaccag gagagggacc
     1681 acgcggtgat ggagatccgc gacgacggac ccggtctcga ccctgaggag ataaaccagt
     1741 gcttcgacag gttctggagg gccgacttct cgaggactac ccccggcttt ggcctggggc
     1801 ttcccctggt aaagaggatc gcccagatac acagtgcctt tatagaggtc gaatccagca
     1861 agggaggggg atccctcttc agggtcaact ttcccctgga ctcggaggcc ctcaaggact
     1921 acgacttcga atagagaaaa aagggggccc ttcaagggcc ccctccggtg cttcttgatg
     1981 acgctacagg tttgccgata cgtagaaggt ccggccctcc cgaaggacaa gcatggcaag
     2041 gctcttcagg tcccttgaga gtatctcctc taactgggca tgatccttga cggtctcacc
     2101 gttgatctcg aggatcacgt cgccctcccg aagacccatc cggtgcccta gcgaccccgg
     2161 aaggacaccg gtgatgatag ctccccctgt ctcctcgatg ccgtacttct ctctcagttc
     2221 aggggtaccg gaagcgatcg agatccccag cttcgacgag ctaacgggct cgtccttgac
     2281 gggggcctcc gacacctcct ccgtcggtac cgccgacaga gtaacgttga tcttcttctg
     2341 gacccccttg cggatgaccg taagctctac cttttctccg gcaaggtgtt gcctgatggc
     2401 tacgataagg tcgccgtgat cttttacctt gcggccgtcg atggcggtga ccacgtcacc
     2461 tctcaggatc ccggccttct ccgccggcgc ccccttcaca acgtcgctga caagggctcc
     2521 ctcacccgac tttatcccgt aggcgctggc gaactccctg gtcagaggct ggaggtatac
     2581 cccgagccaa cccctgttta ccttgccgta ctggataagg tcgtccatga tctgctttgc
     2641 catgttcact ggcaccgcaa agcctatacc ctgggcatag gggacgatcg ccgtattgat
     2701 cccgatcacc tgtccgtcaa ggcctagcag tggtccgccg ctgttgccgg ggttaatggc
     2761 tgcgtcggtc tgtatgaacc cgtcgaagtt gaggttcctg gccctgacgg acctgttctt
     2821 cgtggagacg acgccaacgg ttacgctgct ctcgaaaccg taggggtttc ctatggctat
     2881 gacccactct cctacctcta tcctgtcgga gtcacccatg ggtaccgtcg gcaggtcaga
     2941 agaatcgatc ttaatgaccg ccagatcgta ggacgggtcc tttccgacca ccctggccgg
     3001 gaaggacctg ccatcggaga gagtcacctc tatggtcatg ttgtcgggac cgtccaccac
     3061 atggttgttg gtcaggatat aaccgtcacc agtgacgatg aaacccgaac ccttgcccct
     3121 catgggcact tcccgtgaaa aacggtcaaa ccggtcaccg aagaactctc tgaagaaggg
     3181 atcgtcaggg aagggcgaaa aggacctctg gaccatcgct tcggtatcta tgttgaccac
     3241 tgccggcgaa gcttccttga ctatctttgc gatggggttg cctgtgaaaa cgtcctgtgc
     3301 cgcggctgtt cctgatgaaa ggggtaagac gacggctgcg gaaaggagga acaccagcgc
     3361 cgccaaaaag aaccttgatc ctttggatct tttcatccga gccacctcct gtatgcttta
     3421 cggcaattat cctccctcag ggtcttcctg gaatcgtcaa ttctacggaa atggggtctc
     3481 cgggtttaaa gccgagcatg tccgaggccc tgcccctgtt gaccgcgatg gagacgaaac
     3541 cccaactgtc ctcaagggcg accatatccc cggaacttac gtccgagaag gtgcgtccca
     3601 cagggatgct gaaaagccag ccacagctga cctttatctc cgaatctccc agtttcccca
     3661 ggaggttcca ggggatgtcg gtccgaattg tgccaaaggc gtcaactagg gttacgtggc
     3721 cagagatctt gcccggcgag gactgcggtg ccgggagcgg gaaacggttc agcccgcccc
     3781 tggggatctc gatgccgcac ttttcggggg gaacccctcc gacgaggtcg gccactacgg
     3841 gagccatgat gtcccttgcg tggaatgttg gtgagacctg ctctgcacct gccggcggac
     3901 gctcgatgga ccagctgctg ctgatgccgc cggtcctctc ggccgcgggg acgaggaggc
     3961 cgttgtcggg gccgacgaag aggtctcccc ggacggaacg gatcaccagt atctttctgt
     4021 cggtcccaac accggggtcc actatggcca ggtggacgcc cacaggcatg tacaggaccg
     4081 caccggcgag catccatcca cccgaaatta cgtcgaaggg gggcacctcg tggctgatgt
     4141 caataaccgg cagggtcacc ccccttcgaa gaaggagccc cttgcagcag gccactgtct
     4201 catcggacag cccgaagtca gagagaaagg tgacgcatct gaattcccct gtcatttccg
     4261 aacacccccg ataaatatac catcagcatc tccgggcagg gtaagacaac gcgcccggcg
     4321 gggccggatc acgcaggggt acctgcacaa aaggcaggta atcctcttca ggccttcccc
     4381 tgggagccct tccgggctgg agacactacg actcttctca tgggctcgga accgactgag
     4441 ccggttgaca cgtcgtcccg ttccttgagg gacatgtgga cgatccttct ctcccagctg
     4501 gtcattggtt caagccgtat gggacgcccc tttctctggg cttccctggc ggcggcaagg
     4561 gctatctttt cgatgctatc ctgcctgcgc gccttgtacc cgccgctgtc gatccttatc
     4621 ctccttcccg ggtccttctc ccggaggacc aggttggtga ggaattcgac ggccttgagg
     4681 gtctctccgt tcctccctat tacaattcct gtgtcctccc cggcaaggtc aatattatcc
     4741 ccgtcggtca tcactggctc aatggagagg tccatcttgc caagaagctc ctccaggaag
     4801 gggatagacc cttgcggagg agaagcttca ggccggccca acttcctgga gatggagaca
     4861 cgcatcttct tacccaggag gccgaaaagc cttttgccct cgtcaacgac ctcgatctca
     4921 acatccgaag gttccacgga ccagcggagg gaccctatat gccgggcctc ctccagggaa
     4981 ttcacttcaa caacaaaaga ctcaagctct tccacggcat tgaccccttt cattccagta
     5041 gggaattact gtctgccttc cttcttctgg ggcttttcct ttagaagaac gggcttctcc
     5101 tgcatctcca cctcggtctt tctcttgatg tgccactgct gccccacgcc gatgagcgat
     5161 gagacacccc agtaaaggag cacaccgccg ggcaggctga ggcagataaa tgtgaggaag
     5221 accggcatga accagttcat gaactgcatc tgcggattgg aacttccgct gagtttctgc
     5281 tggaaccagg tgaggtaacc cacgacgagc aggaggacca ggttggccgc gtaaactccc
     5341 acattggcca gaccggccgg gttggcggca atagccgaca ttacctgtcc aatgccgtgg
     5401 gtctctgcca cgaggccaac ggcctctcca agggtggaga ggaccgatcc ctcaagactg
     5461 atcccaagga aggtcgcccc gccaaggtcc aggttcatca acaccctgaa gaggaggata
     5521 agtatgggga gctgcaccag gagtgggaga caacccgccg ccgggttgac gttgttctcc
     5581 ttgtaaagct tcattatctc cttgttcagg gcgtctttgt cgttggcata cttttcctgc
     5641 aggatcttga tgcggggttg aagcttctgc atcttctgca tgcttaccag ctgcttgtgc
     5701 gagagggggt gtagcaggat cctgatcagg atagtcagaa taattatggc tatcccgtac
     5761 gaatgtgtga acgtgtaaag gctgttcagc acgaaaagca ggaaatcgcc cgccgctttc
     5821 cagagtgcgc tcaaaggatt caccttccct tgggagaata ttctctttga tggagaaccc
     5881 tgtcaaataa cgacgggaat tcgtccggaa caggatcgta gccgccgctg tgccagggac
     5941 cgcattttat tatacgcaaa aaagcaagtg ctgttcccct ggccagtcca aacctctcta
     6001 tcccctggat cgcataactc gagcacgagg ggtagaacct gcaccggtca ccaagcaggg
     6061 gggaaatgaa cctctggtag aagtgaatga cctttacggc cgttcttgca ggaagaccct
     6121 tcatccctgg ttgcgcctcc tgtcgatatc ccagtccggc cctggccatt ccggccccag
     6181 gagaccggct ctctggaaca cctttgtcat gtccaagtat acatctcttg cgttctttcc
     6241 tatggctggt gtctttatag tgcagactat ccagaaacct tcctttaccc atggacggag
     6301 cctcctcagg gactcccgca gcagcctttt cccgcgggaa cgcaagggcg aacacccctg
     6361 ctgcttgccc acggcaactc cgtaacgggt cgtcccgtcg ggggaagcca taaacagtaa
     6421 ccgcaccaac gcgccacttt cccggcggcc ggtgcggaaa acctgatcga actgccagcc
     6481 ctttttaagc ctgtcctggg atgggaaaac aaaagaagcc cgcaactaga ccgccaggcg
     6541 cttcctgccc ttgttcctcc tgttctggag gatgcgcctg ccgctcgggc tgctcgagcg
     6601 ctgtaggaag ccgagctttc tctttctctt tctattatgc ggctggaagg tctgtttcaa
     6661 ttgctacacc tcctctggct atcgcttggg aacaccgggg aaaaaccagc ccggatacta
     6721 tatcatacag ggtaacccac aacaagtatt gaagagcaag ctgtggcggg agcaagcaaa
     6781 ggtgtcagcc cgtacacttt tgatatttcc acgttatttt acacggaagt ctttgctcaa
     6841 gctgttgacc cttctccacc atcgcccaga tcgtcgccag gcctggatcc ccccgtcgag
     6901 gcagacatgt tgaccggtgg tggcaatccc tcgccctaac ggcatacctc agccaccaaa
     6961 cccccttatg ctctctcact tttcagaatg gaacagaccc attggtcctg gaacttcccc
     7021 cgggcccact cgtaattgcg caaaagtccc tcggtattga aaccgagatc tttcagcagc
     7081 cccaaagacc ttccattgcc ttcagttaca aaggcctcta tcctgttgag acccatgcca
     7141 tcgaaaccat actccagtgc tgccgacatc gcttccttca tgtacccctt cccccagtga
     7201 tacttgccca gttcatatcc tgtttcagca cggaaatgtt ccttcttcca gttgtggaat
     7261 ccgcaggtcc ccattaccgt tctgtccgaa agcattgtta tcacccacct gatcccttca
//
LOCUS       DAOPKQ010000051         7315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10834_length_7315_cov_3.732645, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000051 DAOPKQ010000000
VERSION     DAOPKQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10834_length_7315_cov_3.732645"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1417
                     /locus_tag="PK146_02885"
     CDS             221..1417
                     /locus_tag="PK146_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE91217.1"
                     /translation="MVMKGIDPSLVEQLRSDAEEAASETIAWRREFHQFPELAFEETV
                     TSSRIAAVLSEMPSVRVIRGFGAPTCVLGILREDIDAPALTLRADIDALAIEEESGLP
                     FASCFPGVMHACGHDAHIATLLGAARILSQRASLLERPVIFLFQPAEEGKGGAKRIVD
                     AGFFREFSIEAVLGLHFWPRLPFGQMVTRKGAITAFSDRVHVTVNGMTAHAASPHFGV
                     DPTVVAAHILLSLQDIISREKDPQSTAVISFGQIETGEAYNIIPGSTHMWGTVRVHDL
                     AVRDFIQKRIEEMVPLVASAHRAMANVEYMRNYPSLVNDGTLTEMVLEKAREFFSSAG
                     GGVAELERPLLAGEDFAFYSLAVPSCFMLIGTGGELSLHNPHYDVPEDLLPLAAAWEA
                     FLALSL"
     gene            1514..2989
                     /locus_tag="PK146_02890"
     CDS             1514..2989
                     /locus_tag="PK146_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPE91218.1"
                     /translation="MNVRLEEKIISQCDRSNGERAIWWRGAWWSRKVLADLALQYETR
                     LKESGFREGNRIAALMPNCPAFLALMISVWKAGGTLMPLNLQAGLASTMDILAHSDVS
                     AVFLPQGMEQLAQGLSSSGLPAIGMPLDGPLPSFQSRSCEKSDPEIAVLFYTSGTTGM
                     PKGVPITHSNLMDNVTKSITHFKDLVDDDIFINVLPNFHTLGFTTSGLLPLLGGYPQA
                     IMPTFMPPENAIEAIKAAEVSVMIAVPTMVALMLGALARGVEPPSSLRLLVTGGDKFP
                     ERLDARTRQLLGVSVLEGYGLTETSPVVAVNEGVARRKLGTVGTVLEGFDVEVRGPEG
                     DVLPAGSEGTLWLRGPSVFGGYFRAPEITREKIRDGWFNTGDVVRIDEEGYVTILDRV
                     TDIIIVGGFNVYPTEIEGVIQQMPGVKECAVVGVQHPVSGEIVKAYVIPSGEPKISAR
                     DVISFSREHLAHYKIPRIVEFIDEMPRSSIGKILKRELRKV"
     gene            3027..4088
                     /gene="citC"
                     /locus_tag="PK146_02895"
     CDS             3027..4088
                     /gene="citC"
                     /locus_tag="PK146_02895"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HPE91219.1"
                     /translation="MFGINIRTFEKLGPEERSLLEGFLQGFGLSWEGHSDLSVLVEDE
                     SGRLIGTGSLSGNVIKMMAVDPEWQEYGLSGTIVSRLVEWGRSRGKTHFFVFTKPGSA
                     DPFTRFGFREIARYDPFAVLLEMGIPGIDQFREYLASCRQKDVTAGKIGGAVVNCNPF
                     TRGHQFLIEKAASLSAHLYVIVVEADLSSFPFRDRIELVRCGTSHLPNVTVLRSGDYA
                     VSPATFPSYFLKDSSKAEIASIQARMDVTLFGNLFVPELGISVRFVGTEPYCPVTGAY
                     NEAMREVLPPLGVDLKVIPRLEIEEGSVVSASTVRSMLKSGNWEIVREMVPDCTWEYL
                     RSDRGRDAIERIRRSQGRH"
     gene            complement(4082..4903)
                     /locus_tag="PK146_02900"
     CDS             complement(4082..4903)
                     /locus_tag="PK146_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPE91220.1"
                     /translation="MKATGLVFDVDGVLIDTSCSFPAAVSGAVRFYLSHFLNFRSSEN
                     IFTGDHYRGAKLHPGFNDDYDIAWIFLSWVASSGEMLEAGTAPSLTDWDRVLALCGDD
                     PPKRAASLFGGVVDRAIVRKICEEIYLGGSISRDLSGQPPTFIDGVGAWKTERPMLCA
                     HWKELGYPVGVYTGRNDIEMGLALEILGWTDLPLQRCITADRGILKPSAEGLFELERI
                     LSVERILFFGDTESDRKAFEGYRRGRFIAVGDLLSGPQAFYSTLEEALSEVLFDQ"
     gene            complement(4900..5529)
                     /gene="hisIE"
                     /locus_tag="PK146_02905"
     CDS             complement(4900..5529)
                     /gene="hisIE"
                     /locus_tag="PK146_02905"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPE91221.1"
                     /translation="MGFDTGSIRFDDAGLVPVILQDISTGEVLMLAWANRQALEETAR
                     RGEMVFYSRSRKEIWHKGATSGNTMKVVDILKDCDNDSLLALVKPSGPACHTGSVSCF
                     NVDGPASSDGATFPGKLWRFLKDRSKASPSDSYTARLLSEGVPRVAQKVGEEGVETAL
                     AAASGDRKAFISEAADLFFHTMVAALAMDVELPEIWSELEERHRERMRE"
     gene            complement(5519..6301)
                     /gene="hisF"
                     /locus_tag="PK146_02910"
     CDS             complement(5519..6301)
                     /gene="hisF"
                     /locus_tag="PK146_02910"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPE91222.1"
                     /translation="MLTRRIIPCLDVKDGRVVKGIRFRELRDAGDPASMAAEYMSEGA
                     DELVFLDISASLSDRGTMLDWVGYVADRLFIPFTVGGGIVDDSQARRIIAHGADKVSL
                     NTAAVRDPDLIRRCASLLGSQAVVLAVDALRTAPGHWDVMVRAGTEPAGIDLLSWVRK
                     ACSLGCGEILLTSIDSDGTLEGYDRECIEAVTSSVQVPVIASGGAGRKEHFLEAFRSG
                     ADAALAASVFHFGTVRIGELKAELSSRGIPMRIEAGGAMNGI"
     gene            complement(6295..7014)
                     /locus_tag="PK146_02915"
     CDS             complement(6295..7014)
                     /locus_tag="PK146_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HPE91223.1"
                     /translation="MKIYPAIDLQDGKVCRLLKGDFKSSVTYPIEPMEAANSFARMGF
                     SHLHVVDLDGARQGAPVHLGLLPRLASTGLSIRFGGGLRTLEDIELALRSGASMAMVG
                     SLLFTSPDMPERIFRSFGDSIIPVVDSRDGFVAHSGWRSLSVISPAKAIEVLSRHGFR
                     DFLVTSTERDGTLLGPDLGLYRSILRSGTFCSITAAGGISSLADLIMLREIGCSGCVI
                     GKAFYEGTIDPVKALEVTGPC"
     gene            complement(7018..>7315)
                     /locus_tag="PK146_02920"
     CDS             complement(7018..>7315)
                     /locus_tag="PK146_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91224.1"
                     /translation="VDILSGTRKLPHVGWNSLEWVVPRPGGIATRPGLDCFYFVHSYG
                     LAPGRDTVATTTVDETCFSSVVKRDRTIGFQFHPERSGFRGLEILSRTLKELGV"
BASE COUNT         1575 a         2093 c         2108 g         1539 t
ORIGIN      
        1 tgccggaact gcgcttgttg tacatccagc acttgagctt gatctcttca ccctcatgtt
       61 tggacagatc gctgatatag acccagggaa ccgtcatgga aagacctcct tcatttcgtg
      121 gcatgtcctg aatgtatgac agtatgatac actaacacat attcgatttt cgggacttga
      181 tgcaaagaga cgatacccga aaagggggtg acggacctcg atggtgatga aaggcattga
      241 cccttctctt gttgaacagc tccgttcaga tgcagaagag gcagcaagcg agaccatagc
      301 ctggcgtagg gagttccacc agttccccga actggccttc gaggagaccg tcacttcttc
      361 ccggatcgcc gctgtcctgt ccgaaatgcc ttcggtgagg gtgataagag gtttcggagc
      421 acccacatgt gttctgggca tcctgaggga ggatatcgat gcccccgccc tcaccctcag
      481 ggccgatatc gatgctctgg ctatcgaaga ggagagcgga ctgccatttg cctcctgttt
      541 cccaggggtg atgcacgctt gcgggcatga cgcccatata gccacccttc tcggtgccgc
      601 caggatactt tctcaacggg cttcccttct cgagaggccc gttattttct tgttccagcc
      661 cgccgaagag ggtaagggtg gggcaaaaag gatcgtcgat gccggattct tccgcgaatt
      721 ttccattgaa gccgtcctgg gccttcactt ctggccaagg ctgcccttcg gccagatggt
      781 cacccgaaag ggggccatca cggccttctc cgacagggtc cacgttaccg tgaacggtat
      841 gacggcccat gcggcgtcgc cccatttcgg agtggacccg acggtggtgg cggcccacat
      901 cctgctctcc ctgcaggata ttatcagccg tgaaaaggat ccccagtcaa cggcagtgat
      961 ctccttcggc cagatagaga cgggggaggc ttacaatata atccccggca gcacccatat
     1021 gtggggcacc gtaagggtcc atgaccttgc tgtcagggat ttcatccaga agaggatcga
     1081 ggagatggtc ccccttgtgg ccagcgccca ccgggcgatg gccaacgtgg aatacatgag
     1141 gaattatcct tcgctagtca acgatggcac acttacggaa atggtcctgg aaaaagccag
     1201 ggagttcttc tcgtctgcag gaggtggagt tgccgaactg gaacgaccgc ttctggcagg
     1261 ggaggatttc gccttttact cgctggccgt cccgtcctgt ttcatgctta tcgggacggg
     1321 aggagagctc agtctccaca accctcatta cgacgtcccg gaggaccttc tccccctcgc
     1381 ggccgcgtgg gaggctttcc tggctctttc tctgtaacat tttcgtgttt caagggggta
     1441 caataggacc gttccaggaa gagaacggtc ccagtgtgga tctcatacat tcttttttcg
     1501 gagggacaat ggcgtgaacg tgaggctcga agaaaagatc atttcccaat gcgacaggtc
     1561 caacggagag cgtgccatat ggtggagagg ggcctggtgg tcccgaaagg tgctggccga
     1621 cctggccttg cagtacgaga ccaggctgaa ggagagcggc ttcagggaag ggaacaggat
     1681 agctgcgctg atgcccaact gtccggcatt tcttgcattg atgatctccg tctggaaggc
     1741 cggcgggacc ctgatgccac tgaacctgca ggccggactt gcctcgacga tggatatcct
     1801 ggcccactcc gacgtatccg ccgtttttct ccctcagggc atggagcagc tggcccaggg
     1861 actttcctct tcgggtctgc ccgccatcgg aatgcctctg gacggccctc ttccgtcctt
     1921 ccagtcaagg tcatgtgaga aatcggaccc cgagatcgcc gttcttttct acacctcagg
     1981 caccacagga atgccgaagg gtgttcccat aacccactcg aaccttatgg acaacgtgac
     2041 caaatccata acccacttca aggaccttgt tgacgacgat atcttcatca atgtgctccc
     2101 caactttcat acgctgggct ttaccactag cggactcctt ccgcttcttg gggggtaccc
     2161 ccaggccata atgcccacct tcatgccccc ggagaacgcc atcgaggcca tcaaagcagc
     2221 ggaagtctcg gtgatgatag cagtaccgac catggtcgca ttgatgttag gtgccttagc
     2281 caggggggtc gaaccgccgt cgtcgctcag gcttttggtc accggaggag acaagttccc
     2341 ggaaaggctt gacgccagga cgagacagtt gctcggggtg tcagtccttg agggttacgg
     2401 gcttaccgag acatcccccg ttgttgccgt gaacgagggg gtggccagga gaaagctcgg
     2461 aacggtcggg accgttcttg aagggttcga tgtcgaggta aggggccccg aaggcgatgt
     2521 cctccccgcc ggttcagagg gtacgctgtg gctcaggggg ccgtcggtct tcggcggtta
     2581 tttcagggca cctgagataa ccagggagaa gatccgggac ggatggttca acaccggcga
     2641 cgtggtcagg atagacgaag agggttatgt gaccatcctg gacagggtaa cggacatcat
     2701 aatcgtgggt gggttcaatg tttaccccac cgagatcgag ggcgtcatcc agcagatgcc
     2761 cggagtgaaa gagtgtgccg ttgtgggggt tcagcacccg gttagcggag agatagtgaa
     2821 agcttatgtc ataccctccg gagagccgaa gatctcggcc agggacgtca tatccttctc
     2881 aagggaacac ctggctcact acaagatacc caggatagtg gagttcatag acgagatgcc
     2941 cagatctagc atcggcaaga tcctgaagag agagctcagg aaggtttgaa gccggcggca
     3001 ctgcggcttc tggaggaaca ggaccattgt tcggcatcaa cataaggact tttgaaaagc
     3061 tgggtcctga agaaaggtcc cttctggaag gattcctaca gggttttgga ttgtcctggg
     3121 agggacattc cgatctttct gtccttgtgg aggatgaaag cggcaggctg atcggaacgg
     3181 ggagcctcag cgggaacgtg atcaagatga tggccgtaga cccggagtgg caggagtatg
     3241 gcctttcggg gaccattgtt tccaggctcg tggagtgggg gcgttcccgc ggcaagacgc
     3301 atttttttgt cttcaccaaa cccggatctg ccgacccctt cacccggttc ggcttcaggg
     3361 agatagcaag atacgacccc tttgccgttc ttctggaaat ggggataccg gggatcgacc
     3421 agttcaggga gtatctggcc tcctgccggc agaaagatgt cacggccggc aagatcgggg
     3481 gtgcagttgt taactgcaac ccatttacca ggggtcacca gttccttatc gaaaaagccg
     3541 catccctttc tgcgcacctc tacgtgatcg tagtcgaggc cgatctttca tcgttcccct
     3601 tcagggaccg tatcgaactg gtaagatgcg ggacttcgca ccttccgaac gtgacagtcc
     3661 tcaggagcgg tgactacgca gtttctcccg ccacttttcc ctcctatttt ctgaaggatt
     3721 cttccaaggc agagatagca tccatccagg caaggatgga cgtaacactc ttcggcaacc
     3781 tcttcgtccc cgagcttggc atctcggtga ggttcgtggg gaccgagccg tactgtccag
     3841 ttacaggtgc ctacaacgaa gctatgaggg aggtccttcc acctctcggg gtcgatctca
     3901 aggtgatccc ccggcttgag atcgaggagg gatcggtagt atcggcctcg acggtaaggt
     3961 ccatgctgaa gagcggcaac tgggagatcg tacgggaaat ggtccctgac tgcacctggg
     4021 agtatctccg ctccgaccgg gggagggatg ccattgaaag gatccgcagg tcccaggggc
     4081 gtcactgatc gaagagaacc tccgaaagag cttcctccag cgttgagtag aaagcctgcg
     4141 gccctgaaag aagatcgccc accgcgataa atctgcccct ccggtaacct tcgaaagcct
     4201 tcctgtcact ctcggtgtcc ccgaagaaga ggatcctctc caccgacagg atcctctcca
     4261 gttcaaagag cccctccgcg gagggtttca ggatcccccg atcggcggtt atgcagcgtt
     4321 gcagcggaag atcggtccat cccaggatct ccagggctag gcccatctcg atgtcgttcc
     4381 tgccggtata gaccccgaca gggtaaccga gttccttcca gtgtgcgcaa agcataggtc
     4441 tttcagtctt ccaggcgccg acaccatcta tgaaggtcgg gggctgccct gacaggtccc
     4501 gggagatcga tcctcccagg tagatctcct cgcatatctt cctgacgata gcgcggtcca
     4561 caacccctcc gaacagtgaa gctgcacgct ttggcggatc gtcgccgcag agagcaagga
     4621 ccctgtccca atcggtcaga gagggggcag taccggcttc gagcatctcc ccggaagatg
     4681 ccacccatga cagaaagatc catgcgatgt cgtaatcatc gttgaagccg gggtgcagtt
     4741 ttgcgccacg gtaatggtcc ccggtgaaaa tgttttcgct gctccgaaag ttcaggaaat
     4801 ggctcagata gaatcgaact gccccagaaa ccgccgccgg gaaggagcag gaggtgtcta
     4861 tgaggacgcc gtccacgtcg aagacgagtc cggtggcctt cactctctca tcctctccct
     4921 gtggcgttct tcgagctctg accagatctc cgggagttcc acgtccattg ccagggcagc
     4981 gaccatggtg tgaaagaaaa ggtccgctgc ctccgatatg aaggccttcc ggtcacccga
     5041 ggctgccgca agggcggtct cgacaccctc ctcgccgact ttctgggcca cccgcggaac
     5101 tccctctgag agaagtctgg ctgtgtagct atcacttggg gaggcttttg agcggtcctt
     5161 caggaacctc cacagctttc caggaaaggt ggctccgtcg gaagaagcgg gaccatctac
     5221 gttgaagcag ctcaccgatc ctgtgtgaca ggccggtcca gatggcttta ccagcgccag
     5281 aagagaatca ttgtcgcagt ccttcaggat gtccacgacc ttcatggtat tgccgctagt
     5341 ggcaccctta tgccagatct cctttcttga cctgctgtaa aagaccatct cccccctgcg
     5401 ggcggtctct tcaagagcct ggcggttggc ccaggccagc atcagtacct caccggtgga
     5461 gatatcctga aggatcaccg gcaccaggcc cgcatcatcg aaacggatcg atcctgtatc
     5521 aaatcccatt catcgcacct ccggcctcaa ttctcatggg tatgccccgg gacgaaagtt
     5581 cagccttgag ttcgccgatc cttacggtcc cgaagtggaa gaccgaagct gccagggcag
     5641 cgtcggcgcc tgagcggaac gcctcgagga aatgctcctt cctgccggct cctcctgaag
     5701 cgatgacagg aacctgaaca gaagaggtta ctgcctcgat gcattcccta tcgtaacctt
     5761 ccagggttcc gtccgaatca atcgaagtga ggaggatctc cccgcaccca aggctgcagg
     5821 ccttccgtac ccaggagagc agatcgatac cggcaggttc cgttcctgcc ctgaccatga
     5881 catcccaatg ccccggagcg gtccggaggg catcgacggc caggaccacg gcctgtgacc
     5941 ccaaaagaga tgcacacctc ctgataaggt caggatccct tacggcagcc gtgttcaggg
     6001 acaccttgtc cgcgccgtgg gcgatgatcc tcctggcctg ggaatcgtcg acgataccgc
     6061 ccccgacggt gaaggggatg aagagcctgt ccgcaacgta accgacccaa tccagcattg
     6121 tgcccctgtc gctaagggag gcactgatgt caaggaagac cagttcgtcg gccccctcgg
     6181 acatgtactc tgcggccatg gaggcagggt ctccggcatc cctcagctcc ctgaagcgga
     6241 tacctttgac aacccttcca tccttcacgt caagacatgg gatgatccgt cttgtcagca
     6301 aggtcccgtc acctccaggg cctttacggg gtcgatggta ccctcgtaaa aggctttgcc
     6361 tatcacgcaa ccgctgcatc cgatctctct gagcatgata aggtcggcca gggaggatat
     6421 cccgcccgcg gcggttattg agcagaaggt accgctcctt agaatggaac gataaagacc
     6481 gaggtccgga cccaggaggg taccgtccct ttctgtggag gttacgagaa aatcacgaaa
     6541 gccgtgcctt gagaggacct cgatggcctt cgcgggggag atcactgaaa gggatcgcca
     6601 gcctgaatgg gcgacaaacc cgtccctgga gtccactact ggaatgatgc tgtcaccgaa
     6661 agagcggaag atcctttctg gcatatccgg tgaggtaaag agaaggcttc cgaccattgc
     6721 catggaggca cccgatctta gggcaagttc gatatcttca agggtcctga ggcctccccc
     6781 aaaacgtatg gagaggcctg tcgaggcgag tcgggggaga agtccgaggt gaacgggggc
     6841 gccctgtctt gcgccgtcaa gatctacaac gtgaagatgg gagaatccca tccgggcaaa
     6901 ggagttggct gcctccatgg gctcaatggg gtaggtaacg ctacttttga aatcaccctt
     6961 aagaagcctg cacaccttcc cgtcctgaag gtcaatggcc ggatatatct tcattgccta
     7021 aacccccagt tccttcaatg tccgagagag aatctcaagc ccccggaagc cgctcctctc
     7081 cgggtggaac tgaaagccga tggtcctgtc cctttttacg acagaggaaa agcaggtctc
     7141 atcgacagtt gttgtcgcca cggtatctct ccccggagca aggccgtagg agtgtacgaa
     7201 atagaaacag tccagcccgg gcctggttgc tatgccgccg ggacggggga ctacccattc
     7261 aaggctgttc catccgacat gaggcagctt tcgggtcccg gagagaatat cgaca
//
LOCUS       DAOPKQ010000052         7261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_10916_length_7261_cov_4.573272, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000052 DAOPKQ010000000
VERSION     DAOPKQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10916_length_7261_cov_4.573272"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..952
                     /locus_tag="PK146_02925"
     CDS             212..952
                     /locus_tag="PK146_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPE91225.1"
                     /translation="MAFYKVRSRTRSWDGVSIGILILDACYPCVPGNVGNATTYDFPV
                     RYKEVQGASIDRLLNERDPSLLEPFIDAARELEAEGVRAITGACGFMALFQRQVAQSV
                     NIPVFISSLLQVPFIYRMLPPGRVVGIITANKSCLTQEHFESVGITPEIPLAVYGMED
                     QYEFRSSILEEKGTMDSDQIEKEVTGVAARMKEENPDLGAVLLECSDLPPYAAAVQEA
                     VDLPVFDFITMIRHVHSAVRRERYSGFM"
     gene            998..2836
                     /locus_tag="PK146_02930"
     CDS             998..2836
                     /locus_tag="PK146_02930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/carnitine O-acyltransferase"
                     /protein_id="HPE91226.1"
                     /translation="MKNLAQYSDMTFGNQRIIPRLPVPGLEETCGKFLEWVKPLLTSE
                     DLERTSEETAFFCSEEGPGPLLQEALVEFASRKDVANWLEPFWDRMYLEGRSPLPLYS
                     NIFYMMSLGTLAGEETQFNRAAGLVEAMIRFKELIESQSLRPDMQGDRPICMIQYKKV
                     FSSCRIPQKDRDILRTPISGENPASPDQRHIVVARNGRFFALDVIDNDGRPYSSHSIE
                     KALAEIVSFESPPEYPPGVLTAVSRDTWAEARAVLRALSPINAGSLDLIENSLFILCL
                     ESRQPKDMKDLSMTMLLGDGRSRWFDKSMQLIVCPGGEAAINMEHTGTDGSVMVRLAG
                     FLTGFESDLREKVPESSKGECSWREIQFHLNNDLREFISRADEECQRMSADTAARVLL
                     FDRFGRDSIKALSAAPDAFIQMALQLAHFDLRGFPANTYEAVMTRQFLHGRTEAVRSV
                     SEESVEFTRLMRGPEVSLEQKATAARKAMEEHVRRTREARDGRGVDRHLFGLWNIYRD
                     MGSDLGIVETPGFFETPGWFTLRRNLLSTSTSGAKGLFLAGFGTVEDEGFGVRYLAFP
                     GSIHFNVTSRTAMMEDMERFVDLLESALLEMEELLSHRSFDQPPSA"
     gene            2928..3131
                     /locus_tag="PK146_02935"
     CDS             2928..3131
                     /locus_tag="PK146_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPE91227.1"
                     /translation="MTTQGTVKWFNDAKGYGFITTDDGKDVFVHFSAIQGDGFKSLAE
                     GQAVSFEITEGTKGPQAANVQKI"
     gene            complement(3246..4196)
                     /locus_tag="PK146_02940"
     CDS             complement(3246..4196)
                     /locus_tag="PK146_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE91228.1"
                     /translation="MKKILALALVMVMALAAGASAQTFLSLATGGTGGTYYPIGGGIA
                     DAVSKALPDLQMTAETGNASQANLNLIGTHQIEMALVQNDVAAFAYDGRFMFQTPYRN
                     VRAVASLYPETVQAIAMKRSGVNSMRDLPGKRVSVGAPGSGVQVDVSLIFRTLGYKYS
                     DVNADFLDFNLTTERMKDGQLDVGFVVAGFPTASIMDLSTMHDITLIEFDDTLIEELH
                     AQYPFFVKDVVPAGTYRGIDKDIQTVAVQAMWVCDGDLPEELVYRVTKAFWENIADVQ
                     KVHAKGQLITIETALDGISVPLHPGAARYYTEIGLKVPTF"
     gene            4494..5060
                     /locus_tag="PK146_02945"
     CDS             4494..5060
                     /locus_tag="PK146_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HPE91229.1"
                     /translation="MRLRELLGTEKDTGPEQASPRERIPSDTPGDHPEADKAPGQKAM
                     ELSVVITLSDDSQIGRSALILASTTGSGEERVLKEQIADFGWKAVATEVGGLAGDLPQ
                     KITRAVVGAALNGEIVRKNSNEMHALMHAAFEAMNGFISVGMLEASIGVKIGIVRNRH
                     WVAVAVVGDTAYHAVAHHERCGLGVMHI"
     gene            5156..5410
                     /gene="rpmE"
                     /locus_tag="PK146_02950"
     CDS             5156..5410
                     /gene="rpmE"
                     /locus_tag="PK146_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPE91230.1"
                     /translation="MKKDIHPEYRKCKVSCACGNSFETGSTVEEIKVAVCSACHPVYT
                     GKRGTRVSEAGRVEKFQKKYAGTRFGKPEESQEAQEDKKD"
     gene            5437..6132
                     /gene="thyX"
                     /locus_tag="PK146_02955"
     CDS             5437..6132
                     /gene="thyX"
                     /locus_tag="PK146_02955"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPE91231.1"
                     /translation="MSISVLLIASTHDPDRTVAAAARLCYSDVSAGDIAEGMKGEDIS
                     RFIGHLRTSGHLSPFEHASFTFAVDGISRVCSHQLVRHRMASYSQQSQRYVDMKEPDM
                     VVPSSVSSDPEVMELFSRTAEEAHEAYRKMIGSGIPKEDARYILPHGWSTKIVVTMNA
                     RELHHFFTMRLCRRAQWEIQDLARKMLILARERSPLLFSIAGPDCVTRGRCFESRPCG
                     QPFSDMEEVLSGC"
     gene            6126..7076
                     /locus_tag="PK146_02960"
     CDS             6126..7076
                     /locus_tag="PK146_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPE91232.1"
                     /translation="MLRPTTFLSLILSLVHSSLLRAREIPVGGQAVIEGVLMRGPGSW
                     ALAVRDPEGGIWHKKWAVRPWNSSFPWDLPVFRGLATMAEMLVTGFRAISLSAQVALG
                     EEEKIGPLELAVTFAVAILGVVGLFVLLPVWISDLGVSYLGLSRSFGHILEGLTRAGV
                     FVAYIAVIGLWKDIARVFAYHGAEHKTINAWESEAPLDRESVSGFSRIHTRCGTSFLL
                     VVVAVSVIVFAAAGHGSLAWRVVSRLMLLPVVIGISYEIIRWCSRNRSLGRALMAPAL
                     WLQYLTTREPDLDQIEVAISALELALEEEGAKSASCGGEN"
     gene            7079..>7261
                     /locus_tag="PK146_02965"
     CDS             7079..>7261
                     /locus_tag="PK146_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPE91233.1"
                     /translation="MEILGKLDELERSLEDIERMMSEPSVASNPAELHRLGKKHSEIV
                     GVVETYREYRKTLSRLE"
BASE COUNT         1619 a         1951 c         2157 g         1534 t
ORIGIN      
        1 gcagggtccg atagccgcgg gttgcggcat cgagggccgt tcccagaccg gtggagaggt
       61 tcccctggtt tcccgggttt ccaccaggct ggggtataag acccggaaca tcggttcttg
      121 tcgagatgga ccagtagtcc cgtccgacgt cggtcaaatg ttccttcagg gagcagagca
      181 tacgaatcca cacagacaag gggagttcaa gatggctttc tacaaggtga gaagcaggac
      241 aaggtcatgg gacggtgtat cgataggaat tctgatcctc gatgcctgct acccctgcgt
      301 accgggcaac gtgggcaacg ctaccacgta tgattttcct gtaaggtaca aggaggttca
      361 gggagcttcc atagacaggc tcctcaatga gcgggaccca tccctcctgg agcccttcat
      421 cgatgccgca agggagctgg aggccgaggg agtaagggcg atcacaggcg cctgcggctt
      481 catggctctc ttccagaggc aagtggctca atcggtgaat atccctgtct ttatctccag
      541 ccttttacag gttcccttca tttacagaat gctgcccccc ggccgggtag taggcataat
      601 tactgccaac aagagttgtc tcacccagga acattttgaa tctgtcggga tcactcccga
      661 gatccccctg gcagtatacg ggatggagga tcagtacgag ttcagaagtt cgatcctcga
      721 ggaaaagggg acgatggact ctgaccagat agaaaaggaa gttacaggtg tagcggcaag
      781 gatgaaggag gagaaccccg acctgggggc cgtactactt gagtgcagcg atcttccacc
      841 ctatgcggct gctgtgcagg aggctgtgga tctcccggtc ttcgacttca ttaccatgat
      901 caggcacgtc cactcggcgg tccgaaggga aaggtacagc ggtttcatgt agaagggaag
      961 agtaacccac ttcgataact aagggaggtc tccaggaatg aaaaaccttg cccagtactc
     1021 tgacatgact ttcgggaacc agaggatcat cccgagactc ccggtacccg gacttgaaga
     1081 gacctgcgga aaattcctgg aatgggtcaa gccgcttttg acttccgaag accttgagag
     1141 aacatctgaa gagacagcgt ttttttgctc tgaggaaggc ccaggcccct tgctccagga
     1201 agctttggtg gagtttgcct ccaggaagga cgtggccaac tggctggaac ctttctggga
     1261 cagaatgtat cttgaaggac gttcccccct ccccctgtac agcaacatct tctacatgat
     1321 gtccctgggc actcttgccg gggaggagac ccagttcaac cgggctgcag ggcttgtcga
     1381 ggcgatgatc aggttcaagg agctcatcga aagccagagc ctgagacctg acatgcaggg
     1441 ggatcgcccc atatgcatga tccagtacaa gaaggtcttc tcctcgtgca ggatcccaca
     1501 gaaggaccgg gacatcctca ggacacccat ctccggagag aaccccgcct cgcctgacca
     1561 gaggcacata gtcgttgccc ggaacgggcg ttttttcgcc ctcgatgtca ttgataacga
     1621 tggcagaccc tacagcagcc actccataga gaaagcacta gctgagatag tctcttttga
     1681 aagccctccg gagtaccctc cgggagtcct cacggccgtt tcgcgggaca cctgggccga
     1741 ggccagggcc gtcctcaggg cactgtctcc catcaacgca ggatcacttg atcttattga
     1801 gaactcactg ttcattcttt gcctggagtc ccgtcagccg aaggacatga aggatctttc
     1861 catgacgatg ctcctggggg acgggcgttc acgctggttc gacaagagca tgcagctcat
     1921 cgtatgcccc ggtggagaag cggcaataaa catggagcat acaggcacgg acgggtcagt
     1981 aatggtaagg cttgccggct tcctgacggg tttcgagagt gacctccggg agaaagttcc
     2041 cgagtcttcc aaaggcgaat gttcatggag ggagatccag ttccatctga ataacgatct
     2101 cagggaattc atctccagag ctgatgagga gtgccagagg atgtccgccg ataccgccgc
     2161 aagggttctg ctgttcgacc gtttcggcag ggactccatc aaggccctct ctgccgcacc
     2221 cgatgccttc atacagatgg ctcttcagct ggcccacttc gatcttcggg gtttcccggc
     2281 caatacctac gaggcggtaa tgaccaggca attcctccac ggaaggacag aagctgtccg
     2341 gagcgtctcg gaggagtcgg tggaattcac caggcttatg aggggaccgg aagtgtcgct
     2401 ggagcaaaag gcgaccgctg caagaaaggc catggaagag cacgtccgaa ggaccaggga
     2461 agcaagggac ggaagaggcg ttgacaggca tcttttcgga ctttggaaca tctatcggga
     2521 tatggggagc gatctgggaa tagtagaaac gcccgggttc ttcgagaccc cggggtggtt
     2581 cacgctcaga agaaacctgc tgtcaacgag tacttctggg gcgaaggggt tgttccttgc
     2641 gggcttcggt actgttgagg acgagggctt cggggtcaga tacctggcat ttcccggaag
     2701 tatccacttc aacgtgacct caaggacagc catgatggaa gacatggaaa gattcgtcga
     2761 tctccttgag tcagccttgc ttgagatgga ggagctttta agtcaccggt ctttcgatca
     2821 acccccatcg gcttgacaaa tcctcccgga taccttattg ttcgctatcg gtgacggatg
     2881 tcaccgaaaa acactttctg gtaacagaag aaatgggagg caggattttg acaacacagg
     2941 gcacagtaaa atggttcaac gacgctaagg ggtatggatt catcaccacc gacgacggca
     3001 aggatgtttt cgtgcatttc agcgcgatcc agggcgacgg cttcaagtcc ctcgccgagg
     3061 gccaggcagt ttccttcgag ataaccgaag ggaccaaggg cccccaggcc gcgaacgtcc
     3121 agaagatcta acctaacctg aaagacaatc gttgaagaga aaagagcggc accctttcgg
     3181 gtgccgctct tcgtgtcttc cctcaggagt ccccttcagg gactgtcctg ttatggtaac
     3241 cgatcctaga acgttggaac cttcaggcct atctcggtgt agtacctggc agcgcctggg
     3301 tggaggggca ccgagatacc gtcaagggct gtctcgatag tgatcagctg gcccttggca
     3361 tggaccttct ggacatcggc tatgttctcc cagaaagctt tggtaaccct gtagacgagc
     3421 tcttcgggaa ggtcgccgtc gcagacccac atagcctgaa cggccacggt ctggatatcc
     3481 ttgtcgatcc ccctgtaagt gccggcaggg actacgtcct tgacgaagaa ggggtactgg
     3541 gcatggagtt cttcgatcag ggtatcgtcg aactcgatca gggtgatgtc atgcatggtt
     3601 gacagatcca tgatcgaagc agttgggaag cctgcaacga cgaacccgac atcgagctgt
     3661 ccgtccttca tccgctcggt ggtgagattg aagtcgagga agtcggcgtt gacgtcggag
     3721 tacttgtagc caagggttct gaatatgagg gagacgtcaa cctgtacgcc ggagccggga
     3781 gctcccacgg agactctctt gccggggagg tcgcgcatgg agttgacgcc agacctcttc
     3841 atggcaatgg cctggaccgt ctcgggatag agagatgcga cggcacggac attcctgtag
     3901 ggcgtctgga acatgaacct gccgtcgtag gcaaaggccg caacgtcgtt ctggacaagg
     3961 gccatctcga tctggtgggt gccgatcagg ttcaggttgg cctgggacgc atttccagtc
     4021 tcagctgtca tctgtaaatc gggaagggcc ttgcttaccg catcggcaat accgccgccg
     4081 atggggtagt aagtgccgcc tgtgccacct gtcgcgaggg acaggaaagt ctgggccgag
     4141 gcgcccgccg ccagggccat aaccataacc agagccagtg caaggatctt cttcatggaa
     4201 ctaccgcctc ctcttcttgt tttcttcagg tcttgatact cgaaaaaaac ccctgtcctg
     4261 caaggccgga tcctgaaacc acctccctgg aggacttcct gcaaccacaa tttcaatgca
     4321 acttcaattt tatacataaa tcacgattgg ttcaagttcc tgtaccgacc tgttcccatg
     4381 atgaagtgca tcacttctgg ggtcccgatt agtatatact ctaatacagg gtcccttgag
     4441 ggatccggcg gcttttcaca ggccatcaat gtgatccggg aggaacaaaa tctatgagat
     4501 tgcgagagct actgggtaca gagaaggata ccggaccgga acaggcaagc ccgagggaga
     4561 ggatcccttc ggatactccg ggagatcatc cggaggcgga caaggccccc gggcagaaag
     4621 caatggaact gtccgtggta ataacccttt cagacgacag ccagataggc agatctgccc
     4681 tgatcctcgc ttcgacgacg ggatccgggg aggagagggt cctcaaggag cagatcgctg
     4741 atttcggctg gaaggccgtg gcgacagagg tggggggcct tgcaggcgac ctgcctcaga
     4801 agatcaccag ggcggtagtg ggggcggcac tgaacgggga gatcgtacgg aagaactcca
     4861 acgagatgca cgcccttatg catgcggcct tcgaggccat gaacggtttc atttcggttg
     4921 gaatgctgga ggccagcata ggggtgaaga taggcatagt gaggaacagg cactgggtcg
     4981 ccgtggctgt cgttggtgat accgcctacc acgccgtcgc ccaccacgag aggtgcggtt
     5041 tgggggtaat gcatatctga agtgcagcgg cgggccttct tgtcttttcc ccgcccgtat
     5101 gttacatttc tcccttgtgg tatggaaaga atgagtatcc atggaggtgt aacagatgaa
     5161 aaaggatatc caccccgagt accgtaaatg caaggtttcc tgcgcctgcg ggaactcctt
     5221 cgagaccggg tccaccgtcg aggagatcaa ggttgccgta tgttcagcct gccatcccgt
     5281 ctacacgggc aagcgcggaa caagggtatc tgaggccggc cgggttgaga agttccagaa
     5341 gaagtacgcc ggtacccgct tcggaaaacc cgaggagtcc caggaagccc aggaagataa
     5401 gaaggactga cccccaggtc cccgagaggt gaacccatgt cgatcagtgt tctgctgata
     5461 gcgagtactc acgatcccga caggacggtg gccgctgcag caaggctctg ctacagtgat
     5521 gtatccgccg gggatatcgc cgagggtatg aagggggagg atatctcccg tttcataggc
     5581 cacctgagga cctcgggcca cctctccccc tttgagcatg catctttcac tttcgccgta
     5641 gacgggatca gcagggtatg ttcacatcag cttgtaaggc acaggatggc cagctactcc
     5701 cagcagagcc agcgttacgt cgacatgaag gaacctgata tggtcgtgcc ttcgtcggta
     5761 tcttccgatc cggaggtcat ggaacttttt tccaggaccg cagaagaggc gcacgaagcc
     5821 tacaggaaga tgatcggatc ggggatcccc aaggaggatg cccgctacat ccttccccac
     5881 ggatggtcaa cgaagattgt ggtgacaatg aatgcccgtg aacttcacca cttcttcacc
     5941 atgcgtctct gccggagggc ccagtgggag atccaggatc tggcccggaa gatgttgatc
     6001 cttgcaagag agaggtcgcc tctgctcttc tccatcgccg gtcccgattg cgtcaccagg
     6061 ggtcgctgct tcgagagcag gccctgcggc caaccctttt ccgacatgga agaggtcctt
     6121 tccggatgct gagacccacc acctttcttt ctctgatcct ttcccttgtc cactcatctc
     6181 ttcttcgggc aagggagata cctgtaggcg gccaggctgt catagaaggc gtcctcatga
     6241 gaggtcccgg gagctgggct cttgcagtca gggatcccga agggggtatc tggcacaaga
     6301 agtgggcggt aagaccgtgg aactcctcct ttccctggga tcttcccgtt ttcagaggcc
     6361 tggcgaccat ggctgagatg cttgtcaccg gtttccgcgc catctccctt tcagcccagg
     6421 tcgcactggg cgaggaagag aagatagggc ctcttgaact ggcagtgacc tttgccgttg
     6481 cgatactggg agtggtaggg ctcttcgtcc ttctgccagt ctggatctcc gacctggggg
     6541 tcagctacct gggcctgagc aggtcttttg ggcacatcct ggaaggcctg accagagccg
     6601 gtgtcttcgt tgcatatatt gccgttatcg gcctctggaa ggatattgcc cgtgttttcg
     6661 cctatcacgg cgccgagcac aagactatca acgcctggga gtccgaggcg ccgctggaca
     6721 gggaatccgt gtccggtttc tcccggatcc acacaagatg cgggacgtcc ttcctgctcg
     6781 ttgtggtcgc cgtgagcgtg atcgtatttg cagctgcagg gcacggctcc ctggcctgga
     6841 gggtcgtttc gaggctgatg ctcctgcccg tggtcatagg tatctcatac gagataatcc
     6901 ggtggtgctc caggaacagg tctctcggaa gagcgcttat ggcccctgct ctctggcttc
     6961 agtacctgac cacgagggag cccgacctgg accagatcga ggtggccata tcggcccttg
     7021 aacttgccct cgaagaagag ggggccaaga gtgcctcatg cggcggtgag aactgacaat
     7081 ggagatattg ggaaaactgg acgaacttga aaggtccctg gaagacatcg aacgcatgat
     7141 gtccgagccc tcggtggctt cgaacccggc agagctgcat cgtctgggca agaagcactc
     7201 ggaaatagtc ggggtggtgg agacctacag ggaatacagg aagaccctct ccaggctcga
     7261 a
//
LOCUS       DAOPKQ010000053         7205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_11027_length_7205_cov_4.816783, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000053 DAOPKQ010000000
VERSION     DAOPKQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11027_length_7205_cov_4.816783"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK146_02970"
     CDS             <1..611
                     /locus_tag="PK146_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPE91234.1"
                     /translation="MPHGTPEGIVPPVSPVLGIRIMSMNLFMEDTSKPVVWRGPLIGN
                     VIGQFWNDTDWVDSEILVIDLPPGTSDAPLTVMQTIALDGVVMVTTPQDISTRIVEKM
                     MHMTRMMNVPLVGMVENMSHAVCPHCGERWDLFGPSHMEEVRSRWDIPQLGSLPIDRQ
                     ITLLADSGKLEEYRSENQPEEMATRVLSAAASLVTYPPDGSR"
     gene            complement(583..1443)
                     /locus_tag="PK146_02975"
     CDS             complement(583..1443)
                     /locus_tag="PK146_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE91235.1"
                     /translation="MKVKEITVVSGKGGTGKTSLTAALAALAPGKLVLCDADVDAPDL
                     EILLHPDPRGEFPFMGMQTAGVDRDRCIGCGKCKEVCRFDAVVMADGRALIDKTFCEG
                     CSACTLVCPAACIAMEDTRQGTWYVGETARGPMVHARLNPGGENSGMLVQLVRKEARN
                     IAGDKGMETILTDGPPGIACPAISAVTGADMALIVTEPTLSGKHDMLRIADLCRRFGT
                     AAAVVLNKADLSSAGSKEIEEACRDNGLPLLASIPFILDVVHAVSQARIPSDELKDHL
                     GTIWSRLGGM"
     gene            complement(1440..2294)
                     /locus_tag="PK146_02980"
     CDS             complement(1440..2294)
                     /locus_tag="PK146_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE91236.1"
                     /translation="MERKALKVAVASGKGGTGKSCVAASLLVTIPGLLGADVDVEEPN
                     LALLLGAEAYASSLVETPWPVIDDGLCDRCGECVRACRFGALYMFGGASPSPNDLCHG
                     CGVCSMVCPKKAISETGRPVGEVRLCRKGDLEIFEGRLRVGMPNPVPVIGAAIEAALA
                     EGRDLVIDSPPGTSCPMVSAVRKADVVVLVTEPTPFGYADLDLALQVIRDLGLPAMTV
                     INRADLGEADIEGLSGRYGVPVAARLPFSRTVAESYAIGDPPALIDPDWREAMVGIWD
                     RARKVAGR"
     gene            complement(2294..2647)
                     /locus_tag="PK146_02985"
     CDS             complement(2294..2647)
                     /locus_tag="PK146_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPE91237.1"
                     /translation="MKIAVATENGNVCPHFGHAPTFRIFETDGKSIIGHQDHPNPGHA
                     PGVLPRWLSEMKVEVIIAGGMGPRAEDLFQAAGVRPVIGATGDAGEAAKAFLEGTLEC
                     GDSACGHVTGEGHSH"
     gene            complement(2755..3069)
                     /locus_tag="PK146_02990"
     CDS             complement(2755..3069)
                     /locus_tag="PK146_02990"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HPE91238.1"
                     /translation="MPGWDGRGPWFGGGPGTGWGTGPCGAGYRRGRGFRRGFGFGRGF
                     GFGRGWGGFGGYAPYPAPGAGAYPAMDPADEKQALQEELGALEAEMKAIRERLDSMEK
                     DV"
     gene            3704..4255
                     /locus_tag="PK146_02995"
     CDS             3704..4255
                     /locus_tag="PK146_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPE91239.1"
                     /translation="MTVLWILLAIAAVLGIYVWRTYNTMVRSRNLVDEGWSGIDVQLK
                     RRADLVLNLVETVKGYAKHEKELFEKVTQARAAAVSAGSVGERAQAENALTGTIRSLF
                     AVAENYPELKANTNFLDLQQNLGNLEAEIQMARRYYNGAVRAYNSLIQVFPNNMLAGP
                     FGFTKRDYFEAAEEDRAVPKVQF"
     gene            4281..6239
                     /locus_tag="PK146_03000"
     CDS             4281..6239
                     /locus_tag="PK146_03000"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPE91240.1"
                     /translation="MGIFRRVLAALLLLFALVPAAAGTERILSFDSSIRVERDGTLSV
                     REDITVRIEHNRIQRGIFRDFPTVYPSPGGGTVRVGFSVQTVLLDGRSVPWKTEGLSN
                     GVRVRIGDPNSYAPRGEHTYTIVYTTTEQIGFFDEHDELYWNVTGTGWEFPIDRASAL
                     VTLPENVPVEKVAWYTGPQGSRAQNAIGKTAGHTAWFQTTSPLGVREGLTIVVGFPKG
                     FIQPSENYLKRQARAAFAQRFGKFIPPLVALIVFIYYMLVWNRYGKDLRPGHIIPLFY
                     PPEGVTPAMASYVYHQKLRDDTFTATLIDLAVRGFLRIEEHDVPKGLFSRAKNRFTLH
                     PIDKDRNSLPVVENAFLISLFPGGASLDVDKANHKELSSAKGAIRDHIADRGKDYFLR
                     NWKWVVLGILITFVLVGFSGLFLGAYGGEIGLSFFATVWLSGWTLGLGAILFTAFTSL
                     KEGIKRKKASFFIRGLFLSAFSVPFLGAEAFFLTMMTDELSPYFTISLVIAFLLNALF
                     FKLMKNYTPEGRKLMDQLEGFRMYMSTAEKERIKAFARVDMPEDTPRHFESLLPYAIA
                     LGVEKEWASRFDQILKAAQYNPDWYAGTYPIYHMGAASFMTGLSGGLGTAVASSSMAP
                     GSKSGFGGGFGGGGFSGGGGGGGGGGGW"
     gene            complement(6282..7058)
                     /locus_tag="PK146_03005"
     CDS             complement(6282..7058)
                     /locus_tag="PK146_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPE91241.1"
                     /translation="MNDHDRRRFLKIYQDVVERQLWDTYAWCLMDNHFHLVIETPEPN
                     LSEGMRLLNGIYTQYFNTNKGRVGHLFQGRFQSIVVDKNTYLMELVRYVALNPVRSGH
                     VDTPEKWTWSSFRATAGLEPCPPWLNADQVINVVSEGIGSKEDRQKSYVDFIMAGIGK
                     KTGLMEKVQQQVYLGDEGFVERVQRKCSVSPDLADISREQKRKPLTRLEDYFKSYPRR
                     KEAMAMAFIEGRFSQAEISRYLGVHYSTVCRAVKEYKEKG"
     gene            6954..>7205
                     /locus_tag="PK146_03010"
     CDS             6954..>7205
                     /locus_tag="PK146_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91242.1"
                     /translation="MKVVIHQTPCIGIPQLAFHNILIDLEEPSPVVIVQKDILLVVPA
                     GHNVVKGSWIFEPKRSGHNKTIIAYKCCNSRSDPKATDLH"
BASE COUNT         1517 a         2224 c         1921 g         1543 t
ORIGIN      
        1 agatgcccca cggcaccccg gagggcatcg tcccccctgt atcaccggtg cttggcataa
       61 ggatcatgtc gatgaacctt ttcatggagg acacctcgaa acccgtggtc tggcgcggcc
      121 cattgatcgg caatgtgatc ggccagttct ggaacgacac cgactgggtc gattcggaga
      181 tacttgtcat agaccttccg ccggggacct cagacgctcc cctcaccgtc atgcagacca
      241 ttgcccttga cggggtggta atggtgacaa caccccagga catctccacc cgcatcgtcg
      301 agaagatgat gcacatgacc aggatgatga acgtcccgct ggtgggcatg gtcgagaaca
      361 tgagccacgc tgtctgtcct cactgcgggg agcgctggga cctcttcgga ccgagccaca
      421 tggaggaagt acgctcaagg tgggacatcc cgcaactggg cagtctccca atcgacaggc
      481 agataacact cctggccgat tcgggaaaac tcgaggagta ccgttcggag aaccagcctg
      541 aggagatggc caccagggtc ctttcagctg ctgcttccct ggtcacatac cccccagacg
      601 gctccagata gtccccaggt gatcctttag ctcatcggaa ggtatcctcg cctgtgaaac
      661 ggcgtggacc acgtcgagaa taaagggaat ggaagcgaga agaggcaggc cgttatcccg
      721 gcaggcctct tctatttctt tcgatcccgc tgaactgagg tccgccttgt tcagcaccac
      781 tgccgccgct gtcccgaagc gccggcagag atcagctatc ctgagcatgt cgtgttttcc
      841 ggacagtgtt ggttcggtga ctatcagtgc catatcggca ccggtcacgg cggaaatggc
      901 cgggcaagct atcccggggg ggccgtcggt gaggatggtc tccataccct tatccccggc
      961 aatgttcctg gcctctttcc tgaccagctg gacaagcatc ccggagttct ctccccccgg
     1021 gttcagcctg gcgtggacca tggggccccg ggctgtctct cccacgtacc aggtcccctg
     1081 cctggtgtcc tccatggcaa tgcaggcggc ggggcatacc aaagtacagg cagagcaccc
     1141 ctcgcagaag gtcttgtcta tcagggccct gccgtcagcc ataacaacgg catcgaagcg
     1201 gcagacctct ttgcacttcc cgcacccgat acacctgtcc ctgtcgaccc ctgctgtctg
     1261 catccccatg aaggggaact ctccccgagg atcggggtgg agcagtatct caaggtctgg
     1321 cgcatccacg tcggcatcgc agagcaccag tttcccgggt gcgagcgccg caagggcagc
     1381 cgtcagggac gtctttccag ttcccccctt gccgctgacg acggtaatct ctttaacttt
     1441 catcttccag ccaccttcct ggccctgtcc cagatcccga ccatggcctc ccgccagtca
     1501 ggatcgatca gggcaggcgg atcaccgatg gcgtaggatt cagccaccgt tctcgagaag
     1561 ggcagcctgg ccgcaacggg gacgccgtac ctcccggaga ggccctcgat atcagcttct
     1621 cccaggtcgg cgcggttgat caccgtcatg gccggcaagc ccaggtcacg gatcacctgc
     1681 agcgcaaggt caaggtcagc atagccgaag ggcgtcggct ccgttaccag aaccacaaca
     1741 tcggccttcc tcaccgcgga caccatcggg caggaagtcc ccggaggaga atctatgacg
     1801 agatcccgac cctctgccag cgccgcctcg atggcggcgc ctatcacggg tacggggttg
     1861 ggcattccga cgcggagccg cccttcaaag atctcgaggt cgccctttct gcatagcctg
     1921 acctctccca cgggtcttcc ggtctcggag atcgcctttt ttgggcagac catggagcag
     1981 accccgcagc cgtggcagag gtcgttggga gacggtgaag ccccgccgaa catatagagc
     2041 gccccgaagc ggcatgccct tacgcattct ccgcagcggt cgcacaggcc gtcatcgatc
     2101 accggccagg gggtctccac cagggaggag gcatatgcct cagcccccag aagcagggcc
     2161 agattgggtt cctcaacgtc gacatcggcg cccaggagtc cggggatggt gaccagtagc
     2221 gaggcggcca cgcagctctt gcccgtgccg ccctttccgc tggcaaccgc gaccttaagt
     2281 gcctttcgtt ccatcagtga gaatggccct cgccggtaac gtggccgcag gcgctgtccc
     2341 cgcattcaag ggtaccttcg agaaaggcct ttgcggcctc accggcatca cccgttgctc
     2401 ctatcactgg ccttacgccc gctgcctgga acagatcctc ggcccggggt cccatcccgc
     2461 cagcaataat cacctccacc ttcatctccg aaagccatcg gggcagcact cccggagcgt
     2521 gacccgggtt cgggtggtcc tggtggccaa ttatgctttt tccgtcagtc tcgaagatcc
     2581 tgaaagtagg ggcatggccg aagtgggggc atacattgcc gttctcggta gctactgcta
     2641 tcttcatttt ttcggtcctc ctgatgtaaa aaaggatttt tgatgcctgc ttacaacaga
     2701 tggacccggt ccatcaggac cgggtcccga agaaaaggag atggttgtag cggatcagac
     2761 atccttttcc atactgtcca gccgttccct tattgctttc atctctgcct ccagggcgcc
     2821 aagctcctcc tggagagcct gtttctcgtc cgcggggtcc atagccggat aggctcctgc
     2881 tcccggggcc gggtagggtg cataaccccc aaaaccaccc cagcctctac cgaacccgaa
     2941 acctctccca aatccgaaac cacggcggaa accacgcccg cggcggtatc cggctccaca
     3001 ggggcccgta ccccatcctg taccaggtcc gcctccgaac caggggcctc ttccatccca
     3061 accgggcatg accatcgcct ccttttattg atattggtta tcaatagaat atcacctttc
     3121 ccccccttgt caaccccttg gggtcagacc ttgaattgca gcaattgtgt acaaacatgg
     3181 attttcattc aagggccaag actcagttag acggcaagag caatacaaaa ctgataaaca
     3241 aggtgcgtta atggttgacg gccagggttt ttcggaggtc ttcctgatct gtccccaaat
     3301 attggtcctc aaagaattga caattttata aaatttgtta ttctttagct ggcgacagtg
     3361 aacgttatct gcatcacaag gataatgggc ctcaataaaa gaagataatg caaaggctac
     3421 agagagagca cgaagaaata atgtgaatct tcacggttgc aacttccacc ataatcggta
     3481 cgaccttcgg actaaactag cttttggcca actacctctg gcacctcctt tcaatagaag
     3541 caccaccata gataacggcc ccggagactc cggggccgtt cttatttgtc ccctcgttca
     3601 ccgttcacaa agtgccccag ggttttcctg atgcctgtcg cctgtcgcct tgctttaagg
     3661 tatattatcc ttaacgcacc gaccaattcc gggggtgatc tgcatgaccg tcctatggat
     3721 ccttctcgct atcgcagccg tactgggcat ttatgtctgg agaacataca acaccatggt
     3781 ccgttccagg aaccttgtcg acgaaggttg gagcggcatc gacgtccagt taaaacgtcg
     3841 tgccgacctg gtgctcaacc ttgtcgagac cgtcaagggc tacgccaagc acgaaaagga
     3901 actcttcgag aaagtaaccc aggccagggc tgcggcggta agtgcaggct cagtgggtga
     3961 gagggcccag gcggagaacg ccctcaccgg gaccatccgc tcccttttcg ccgttgccga
     4021 gaactacccc gagctcaagg cgaacaccaa cttccttgac ctgcagcaga acctcgggaa
     4081 cctggaggca gagatccaga tggcccgcag gtactacaac ggggctgtca gggcctacaa
     4141 ctccctgatc caggtcttcc cgaacaacat gcttgccggg cccttcggct tcaccaagcg
     4201 tgactacttc gaggcggccg aagaggaccg agccgtcccg aaggtgcagt tctagtcata
     4261 tcacggcggg ggcttcccca atggggatat tcaggcgtgt ccttgctgcc cttctgttgc
     4321 tttttgcgct ggtcccggcg gcggccggga ctgaaaggat actgtccttt gacagctcca
     4381 tccgggtcga aagggacggc accctgtccg ttcgcgagga tatcacagtc cgcatcgagc
     4441 ataaccgcat ccagaggggt atcttcaggg atttccccac tgtctatccc tcccccggag
     4501 gaggcaccgt ccgtgtcggc ttctcggtgc agaccgttct gcttgacggg cggtcagtcc
     4561 catggaaaac agaaggcctc agcaacggtg tcagggtccg gataggcgac cccaactcct
     4621 acgcaccccg aggggagcac acctacacta tcgtctacac tacaactgag cagataggat
     4681 tcttcgatga acacgacgaa ctctactgga acgtgacggg caccggctgg gagttcccca
     4741 tcgatcgggc ttcagccctt gtaacgcttc cggagaatgt tcccgttgag aaagtggcct
     4801 ggtacacagg tccccagggg tcgcgagccc agaacgccat cgggaagacg gcggggcaca
     4861 ccgcctggtt ccagaccacg tccccgctag gggtaaggga gggtctcacc atagtggtgg
     4921 gcttccccaa gggattcatc cagccctcgg agaactacct taaaaggcag gcccgggcgg
     4981 ccttcgccca gcgcttcggc aagttcatcc cccccctggt ggcccttatc gttttcattt
     5041 actacatgct ggtatggaac cgatacggca aggaccttcg acccggacac atcatccctc
     5101 tcttctatcc gcccgaaggg gtcactcccg ccatggcctc ctacgtctac caccagaagc
     5161 tccgagacga cacattcacc gccaccctca tcgaccttgc cgtaagaggc ttcctcagga
     5221 tcgaggagca cgacgtaccc aagggcctct tcagcagggc caagaaccgc ttcacccttc
     5281 accccataga caaggaccgt aacagccttc cggtcgtgga gaacgcattc ctgatctccc
     5341 tcttccccgg cggggcttcc ctggacgtgg acaaggccaa tcacaaggaa ctctcctcgg
     5401 ccaagggcgc catcagggac cacattgccg accgcggaaa agactacttc ctgaggaact
     5461 ggaagtgggt cgtcctgggc atcctcatca cctttgtcct ggtaggcttc agcggattgt
     5521 tcctgggggc ctacggaggg gagatagggc tatccttctt cgccactgtg tggctgagcg
     5581 gctggaccct gggtctgggc gccatactct ttacggcctt cacctcgctc aaggagggta
     5641 tcaagcggaa gaaagcaagc ttcttcatcc gggggctgtt cctttcggca ttctcggtgc
     5701 ctttcctggg agccgaggcc ttcttcctga cgatgatgac cgatgaactg tcgccctact
     5761 tcactatctc cctggtgatc gccttccttc tgaacgctct tttcttcaag ctaatgaaga
     5821 actacacccc cgagggcaga aaactgatgg accagctcga gggcttccga atgtacatgt
     5881 ccactgcaga aaaagagagg ataaaggcct ttgccagagt ggacatgccc gaggacacgc
     5941 ctcggcattt cgagagcctg cttccctacg cgatcgccct gggtgtggag aaggagtggg
     6001 cttccaggtt cgaccagatc ctcaaggcag cccagtacaa ccccgactgg tacgcaggaa
     6061 cctaccccat atatcatatg ggcgcggctt ccttcatgac gggactcagc ggaggtcttg
     6121 gaacggcagt tgcgtcatct tccatggctc cgggatccaa gtccggtttc ggcggcggtt
     6181 tcgggggggg aggtttctca ggtggcggtg gaggaggagg tggcggcgga ggctggtaga
     6241 ccctttgggg tcagaccttg aattgcagca atggtataca tttaaccctt ttctttgtac
     6301 tccttaactg ccctgcatac agtgctgtaa tgtactccca ggtaccgtga tatctcggcc
     6361 tgactgaacc ttccctcaat gaaggccatg gccatcgctt ccttccgccg gggataactt
     6421 ttgaagtaat cctccagtcg ggtaaggggc ttcctctttt gttccctgga aatatctgcg
     6481 agatcaggac ttaccgaaca cttcctctgc accctctcga caaatccttc atcgccaagg
     6541 tagacctgtt gctggacttt ctccatcagc ccagtctttt tgccaatgcc agccataata
     6601 aaatcaacgt aactcttctg gcggtcttcc ttgctcccga tcccctcgga aaccacattg
     6661 ataacctgat ctgcgttcag ccagggcggg cagggttcaa gtcctgctgt tgccctgaaa
     6721 ctgctccagg tccatttctc gggagtatca acatggccgg aacgcaccgg gttcaaagcc
     6781 acatatctca caagctccat gagatatgtg ttcttatcca cgactattga ttgaaacctt
     6841 ccctggaaga ggtgtccgac ccttcccttg ttggtgttga aatattgtgt gtatatcccg
     6901 ttcaggagcc tcatgccttc cgacaggttc ggctcaggtg tttcaattac aagatgaaag
     6961 tggttatcca tcaaacacca tgcataggta tcccacaact ggcgttccac aacatcctga
     7021 tagatcttga ggaaccttct ccggtcgtga tcgttcaaaa agatattctg cttgttgtcc
     7081 ccgcgggaca taacgtggta aagggctcct ggatattcga gcctaagagg tctggtcata
     7141 ataaaacaat aatagcatac aaatgctgca attcaaggtc tgaccccaaa gcaacggacc
     7201 ttcat
//
LOCUS       DAOPKQ010000054         6962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_11555_length_6962_cov_5.048936, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000054 DAOPKQ010000000
VERSION     DAOPKQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11555_length_6962_cov_5.048936"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PK146_03015"
     CDS             <1..945
                     /locus_tag="PK146_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPE91243.1"
                     /translation="GGKKVPPWHSIPGGVNRSLKSAEVDTIRVQIPEMKAAVKEAIEL
                     VKGYLEKNAGKAASFATLETAYMGLVQDGFLEIYDGDIRLKGPRGRIIDEFPDWAYLD
                     FIGEHVEPWSYLKFPFYRPMGFPHGSYRVGPLGRVNACDDISTPGASAELAEFRKGAE
                     DGMVHKTLYYHYARLIETLYGVERIEELIEDPDITGSDLRVTSTELYPEGIGVIEAPR
                     GTLIHHYQIDENGVLTGVNLIVATGHNNYAMSKGVDMVARENIKGGEPTEGILNSMEH
                     VIRCYDPCLSCSTHAVGRMPLHLEIFGADGNLKGEIKN"
     gene            969..1403
                     /locus_tag="PK146_03020"
     CDS             969..1403
                     /locus_tag="PK146_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPE91244.1"
                     /translation="MFGYGNPFRQDDGAGHHLATLLARDLEKAGKRTRLWLGHQLLPE
                     AALGIEPDDILLFVDASLEEYDHGFALERVRPDAGSNHGLNIHSFGPSWFLTLLGDLG
                     TGFGDAWVLSITGRSFDFGEGLSDECRRRSELARTAFREKFC"
     gene            1530..2213
                     /locus_tag="PK146_03025"
     CDS             1530..2213
                     /locus_tag="PK146_03025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPE91245.1"
                     /translation="MTGNDQHLSTDHVRHQEKPQGPSENSLVFVRHGEPDLTSSLTIF
                     LGRVGPGLSPRGKAQADKVSLWARSFSWRGCFCSPLVRAKETAEIICSSLDIEPVIKE
                     ELSEIDLGCWDGREKTDIPREYPRLWEQREKDLYSFKHPGGESFADLEARVVPLLRSF
                     AVKGGRWLIVSHAGVFRVMLRTLFEIPFPETFRYDPEYGGVLLLEKEGEAFLARGLQG
                     LRIAWREPA"
     gene            2210..3337
                     /locus_tag="PK146_03030"
     CDS             2210..3337
                     /locus_tag="PK146_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPE91246.1"
                     /translation="MTDVRLRTGGIVLAGGLSSRMGRCKALLEIAGSSALDQVLSSMR
                     QAHVEDLVVVSGFHRKEIEKEATGGGVRTVFNDRFEEGMLSSVKTGVRALSDDLEAFF
                     LLPVDIPLVRPATLREMKKSFSSNLGLEAVFPAFLGQRGHPPLIRGDLIPGILRWEGP
                     MGLRGFLEQHEASSLTLDTPDEGTVMEMDTFEEYQELRSLAIRRNVPTERECLAFWEI
                     LSTPQEVREHSRVVGEVAVRLANLVRDTDHVDPELLKAAALLHDMARTLGHHADVAAD
                     MLELWGFAETADLVRYHMELPEDEPLLSGRSLLYLADKLTSGNRVVDLRQRRKLIEKR
                     FSSDPDALAAALVRLQRARTIANSFEKASGTKLEATLAYRN"
     gene            3385..3543
                     /locus_tag="PK146_03035"
     CDS             3385..3543
                     /locus_tag="PK146_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91247.1"
                     /translation="MLPTLDLDRDLSGNTCDLPELLQHTLHYVPELPIVAMRKIIDCE
                     TAMKRDAS"
     gene            complement(3646..4158)
                     /gene="tsaA"
                     /locus_tag="PK146_03040"
     CDS             complement(3646..4158)
                     /gene="tsaA"
                     /locus_tag="PK146_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPE91248.1"
                     /translation="MEPIKYSAIGIIRTPHRSREGTPIQPSGARGVLGRIELYEDLRE
                     GLKDLEGFSHLILLYHFHLSEGYELAVKPFLDQTVHGLFSTRAPRRPNALGLSVVRLV
                     SIIDGVLVVDGVDMVDGTPLVDIKPFVPDFDVPGGEIRTGWLERNSERARAIRADTRF
                     TGDIKLPPES"
     gene            complement(4173..5279)
                     /locus_tag="PK146_03045"
     CDS             complement(4173..5279)
                     /locus_tag="PK146_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE91249.1"
                     /translation="MDLLSVRNLSVDLGNFSLKDISFGVPEGSYCCLLGASGAGKSVL
                     LETIIGAFRPKQGKVYLRGTDVTAWPPERRRLGIVYQDYMLFPHLDVRGNIAFGLKGQ
                     GLTTDKIDPRVMKISRQLDIEDLLERDIATLSGGEQQRVALARALVTEPEILLLDEAF
                     SALDVNTRERLRTMMARVVREIGITVLHVTHDREDVWALATHVAVINDGEMLQHGTPE
                     DLFRRPRPGHVASIMGAGNIISGEVVSETGADLGIVLANGIRLYSSENLTMGSDVTLS
                     IRPEDILVARDESVISARNRIPVTLSEIKRRGPLTWLTGRSGDLELIAMVTSSGYESL
                     ETSPGDMCFFIFKASSLCLIEENFQKIVLASNFS"
     gene            complement(5280..6065)
                     /locus_tag="PK146_03050"
     CDS             complement(5280..6065)
                     /locus_tag="PK146_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91250.1"
                     /translation="MSNRIFKAALGGVTVLFSGLLIWAILSLILMPEVSEFVESTLSE
                     EMLYSIRLSLFTSLISVALVMVLAVSIGFVLARFTFPGHNLLRTIVDLPMAFPELVLG
                     LCLLLLFGHAPFGKWLQSMGVNVVFSKYSIVVAQVFTAAPYAIRVVYGTFRGISPRYE
                     MVARSLGCGLLGSFLRVSVPMAKGGLFAAAVIAFARCMGCFGTVLILAGGTRMYTETL
                     PITLYLNISYGNLAMAMSSGIILIFISLGAIIVFEVLNQEVVL"
     gene            complement(6072..6854)
                     /gene="modA"
                     /locus_tag="PK146_03055"
     CDS             complement(6072..6854)
                     /gene="modA"
                     /locus_tag="PK146_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE91251.1"
                     /translation="MRKLVTAMLLLVLSLSVSFVPVAVATEEPLVVYCGAGLMKPMDE
                     LKEAFEQEAGIPVRMIYAGSGELFGMISTRQEGDVYIPGDIKYTNDALEKGISIKDGM
                     ETICYHVPVILVPAGNPGKVNSLEDLGKEGVRVALADEKAAAIGNVTVALLKKNNILD
                     QVTKNVVTKPSTTNQLLIYTATAQVDATIAWEDQALWGEGKGKIEVVRIPSEKNLIKT
                     IPASVVTYTRRPEDAKAFIRFITSEESRSVWDNWGFPIEKPQ"
BASE COUNT         1593 a         1987 c         1924 g         1458 t
ORIGIN      
        1 ggtggcaaga aggtcccccc ctggcacagc atccccggag gcgttaacag gtcgctcaag
       61 tctgcagagg ttgatacgat ccgtgtccag atccccgaga tgaaggccgc agtgaaggaa
      121 gctatcgaac tggtgaaggg ctacctcgaa aagaacgccg ggaaggccgc ctccttcgcg
      181 acccttgaga cagcatacat gggcctcgtg caggacggat tcctggaaat atacgacggt
      241 gacatccggt tgaagggacc ccgggggcga ataatcgacg agttccccga ctgggcctac
      301 ctggatttca taggggagca cgtagaaccc tggagctacc tcaagttccc cttctacaga
      361 cccatgggat tcccccacgg gagctaccgc gtggggcccc tggggagggt caacgcatgc
      421 gacgacatct ccacccctgg agcgtcggcc gaactggcag aattccgcaa gggagcggag
      481 gacggcatgg tccacaagac cctctactac cactacgcaa ggcttatcga gaccctctac
      541 ggcgtagaga ggatcgagga actcattgaa gatcccgaca taacaggcag cgatctgagg
      601 gtcacctcta cggagctcta ccccgagggg ataggggtca tcgaggcgcc aaggggaacg
      661 ctgatccacc actaccagat agatgagaac ggggtcctga caggagtgaa cctcatcgtc
      721 gccacggggc acaacaacta cgccatgagc aagggcgtag acatggtcgc cagggagaac
      781 atcaaaggtg gcgaacccac ggagggaatt cttaacagta tggagcacgt catccgctgc
      841 tacgacccct gcctctcctg ctcgacccat gctgtgggga gaatgcctct ccacctggag
      901 atattcggag cagacgggaa cctgaaggga gagataaaga actgacggac aggagagcat
      961 cgttcttcat cttcggatac ggtaaccctt tcagacagga tgacggtgca ggtcaccacc
     1021 tggccacgct tctggccagg gacctggaga aagcaggaaa gaggacccgt ctctggctcg
     1081 gacaccagct cctgcccgag gccgcccttg gtatagagcc ggacgatatc ctgctcttcg
     1141 tggatgcctc gctggaagag tacgaccatg gattcgccct tgaaagggtc cgcccggatg
     1201 cgggatcgaa ccatggcctg aacatccact ccttcgggcc ttcatggttc ctgacgctgc
     1261 ttggagacct gggcacaggg ttcggcgatg catgggtcct gtcgataacg ggcaggtcct
     1321 ttgatttcgg ggaaggactc tccgatgaat gcagaagaag gagcgaactg gcaagaacgg
     1381 ctttcaggga aaagttctgc tgatccgtga agctctcttg tattgactgc cggaccgacc
     1441 catgtgctgt ctgcagctga tgcggctaag atcggtccgg ctttctttca ggaactcgct
     1501 gtaattggaa ggcggtggat cccccttcaa tgaccgggaa cgatcagcac ttatcgacag
     1561 atcacgtccg ccaccaggaa aagcctcaag gaccatctga gaacagcctg gtattcgtca
     1621 ggcacggaga gcccgatctg acctcctcgt tgacaatctt tctgggtcgt gtcgggcccg
     1681 ggctctcccc gcgaggcaaa gcccaggcgg ataaggtgtc cctctgggca cgctctttct
     1741 catggagagg gtgcttctgc agtcctctcg tgagggcaaa ggaaacggca gagattattt
     1801 gcagctccct tgatatagaa ccggtgatca aagaggagct gtcagagatc gacctcgggt
     1861 gctgggatgg acgggaaaag acagacatcc ccagggagta tccccgatta tgggagcaaa
     1921 gagaaaaaga tctttactct ttcaagcacc ccggaggaga gtcatttgcc gacctggagg
     1981 ccagggttgt tccgctcctc cggtccttcg ccgtgaaagg aggaagatgg ctgatagtat
     2041 cccacgcagg cgtctttcgt gttatgcttc gaaccctttt tgagataccc ttccccgaaa
     2101 ccttccgcta tgaccccgaa tacggcggag tattgctcct cgaaaaagag ggggaagctt
     2161 ttctggcaag aggcctccag ggcttaagga tcgcctggag ggaaccggca tgaccgacgt
     2221 ccgactccgg acaggtggca ttgtgctggc tggagggctt tcgtcgagaa tgggaagatg
     2281 caaggccctt ctggagatcg ccggctcctc ggctcttgat caggttctat cctccatgcg
     2341 ccaggcccac gttgaagatc ttgtggtcgt ttcaggtttc catcgcaagg agatcgaaaa
     2401 ggaagcgacg ggcggcgggg ttcgaactgt gttcaacgac cgtttcgagg aggggatgct
     2461 ctcgtcggtt aagactggag taagggctct ttctgatgac ctcgaggcct ttttccttct
     2521 tcctgtcgac atccccctcg taaggcctgc caccctccgg gagatgaaga agtccttctc
     2581 gagcaacctt gggcttgagg cggtcttccc ggcttttctg ggacagaggg ggcatccccc
     2641 tctgatccgg ggcgatctga tcccaggcat cctgagatgg gaaggaccta tgggattgcg
     2701 aggattcctt gagcagcacg aagcaagctc cctgaccctg gacacgcccg acgagggaac
     2761 agtcatggag atggacactt ttgaagaata ccaggaactg agatccctcg cgatcagaag
     2821 aaatgtcccc actgaaagag agtgcctcgc cttctgggag atcttgtcta ctccgcagga
     2881 ggttcgtgaa cactcccggg tcgtcgggga ggtggcagtc agactggcga atcttgtaag
     2941 ggatactgat catgtggatc ctgaactcct gaaggctgct gcgctgctcc acgacatggc
     3001 ccggactctt ggccatcatg ccgatgtggc cgccgacatg ttggaactat ggggttttgc
     3061 cgagaccgcg gatcttgtca ggtaccacat ggaactccca gaagacgagc ctcttctttc
     3121 gggaagatcc ctgctttatc ttgctgacaa gctcacctct ggcaacaggg tggtagacct
     3181 caggcagaga agaaaactga tagagaaaag attctcgtca gacccggatg cccttgctgc
     3241 ggcgcttgtg aggctgcaaa gagcaagaac aattgctaat tctttcgaga aagcctcagg
     3301 gacaaagctg gaggcaacac tggcataccg gaattgagaa ccgggagtca gttatcccga
     3361 tccctttttg gcccctgtga aataatgctc cccaccctcg atcttgaccg tgatctgtcc
     3421 gggaatacct gtgatcttcc tgagcttctg cagcacactc tccactatgt ccccgaatta
     3481 ccgatagtgg caatgcggaa gatcattgat tgtgagaccg cgatgaaacg ggacgcctcg
     3541 taagccaggt ccctgggggg aaaagagaag cttcctgaag atgacagggg accccccctt
     3601 cagggatccc ctttgaacgg atcaccgtgt aaacttcgct tgaggtcaac tctctggagg
     3661 cagttttata tccccggtaa aacgcgtgtc cgctcttata gccctggccc tttctgaatt
     3721 ccgctccagc caccctgtac ggatctcccc tccggggaca tcgaaatcag ggacaaaagg
     3781 tttgatgtcg accaaagggg taccatccac catgtccact ccatcaacga caaggacccc
     3841 atcgatgatc gatacaagcc ttaccactga aaggcctaac gcatttggtc tcctgggtgc
     3901 cctggtcgaa aaaagaccgt gaaccgtctg gtccagaaaa ggttttacag caagttcgta
     3961 gccttccgaa aggtgaaaat gataaagaag gatcaggtga gagaatcctt ccaggtcttt
     4021 aagaccttcc ctgagatcct catacaactc tatgcggccc aatacacccc tggctcccga
     4081 cggttgtatg ggtgtcccct ccctggacct gtgaggggtc cggatgatcc cgatggctga
     4141 gtattttata ggttccataa ctatctcccc ggttacgaaa aattcgatgc caatactatt
     4201 ttctggaagt tctcctcgat caggcagagc gaactcgcct tgaagatgaa aaagcacatg
     4261 tccccggggg aggtttccag ggattcatag ccgctcgagg tgaccatcgc aataagttca
     4321 agatcccctg atcttcctgt cagccaggtc agcggccccc ggcgctttat ctctgaaagt
     4381 gtaacgggta tcctgttcct ggccgagatg accgactcat ccctggcaac caggatatcc
     4441 tctggcctta tcgacagggt aacatcgctc cccatggtca ggttctccga cgaatagagc
     4501 cggatcccgt tggcaaggac aatacccagg tcggccccgg tctcactgac cacctccccg
     4561 gaaataatgt tgcctgcgcc cataatgctg gccacgtgcc ccggcctggg acgcctgaaa
     4621 agatcttcgg gagtcccgtg ctggagcatt tccccatcgt tgatgacagc cacgtgggtg
     4681 gccagggccc atacatcttc ccggtcatgg gttacatgca gaacggtaat gccgatctca
     4741 cggacgaccc gggccatcat tgtgcgaagc ctctccctgg tattcacgtc aagagcgctg
     4801 aaagcctcat cgagcagaag gatctccggt tcggtcacga gggccctcgc cagggcgaca
     4861 cgctgctgct ctcctcccga gagagtggca atatcccgtt caagaagatc ttcgatgtca
     4921 agctgcctgg agatcttcat gaccctggga tcgatcttgt cagttgtcag cccctgacct
     4981 ttaagcccga aggcgatgtt gccccggaca tccagatggg ggaaaagcat atagtcctgg
     5041 tagacaatgc ccagcctcct cctttcaggc ggccatgctg tcacgtcggt ccccctgagg
     5101 tagaccttcc cctgtttcgg gcggaaagca cctatgatcg tctccaggag cacgctcttg
     5161 ccggcgccgg aagcgcccag caggcagcag tacgaccctt ccggcacccc gaaggatata
     5221 tccttgaggc tgaagttgcc caggtcgacg gagaggttcc ttacagaaag caggtccatc
     5281 tacaggacca cctcctggtt cagaacctcg aatacgatga tcgccccgag ggatatgaat
     5341 atcaggatga tccctgaaga catggccatg gccaggttgc cgtaggatat attcaggtaa
     5401 agggttatgg gaagggtctc ggtgtacatt cgggtgccgc ctgccagtat gagcaccgtc
     5461 ccgaaacatc ccatgcaccg ggcaaaggca atgaccgccg cagcgaaaag gccgcccttg
     5521 gccatcggga cggagaccct taaaaaactg cccagcaacc cgcagccgag gctccgggcg
     5581 accatctcgt acctcgggct gatccccctg aaggtaccgt agacgaccct gatggcgtac
     5641 ggagctgctg tgaagacctg ggcgactacg atggaatatt tgctgaaaac cacgttcact
     5701 cccattgact gaagccactt cccgaaaggt gcatgaccga aaaggagaag gaggcacagc
     5761 ccaaggacca gctcagggaa cgccatgggc aggtcgacaa tggttctcag aaggttatgc
     5821 cccgggaagg tgaatcgggc cagtacaaaa cctatcgaaa cggcgagcac catcaccagc
     5881 gccacggaga tcaggctggt gaaaagagac agccttatgc tgtagagcat ctcctcggaa
     5941 agggtgctct cgacgaactc cgatacctcg ggcataagga taagggaaag gatcgcccag
     6001 atcagcagcc ccgagaacag gactgtcact ccccccaggg ctgccttgaa tatcctgttg
     6061 ctcataactg tctattgagg cttctctatc gggaaacccc agttatccca gacagatctg
     6121 ctctcttcgg aagtaatgaa acggataaag gccttcgcgt cttccggtct cctggtatag
     6181 gtaactaccg atgcgggtat cgtcttgatc aggttctttt cagagggtat ccgtaccact
     6241 tctattttcc ccttgccctc gccccagagg gcctggtctt cccaggctat ggtcgcatcc
     6301 acctgggccg tcgcggtgta tatcaacagc tggttggtgg tgctcggttt tgttacaaca
     6361 ttttttgtga cctggtccag gatgttgttc ttcttcagaa gagctacggt cacattccct
     6421 atggctgccg ccttttcatc cgccaaagcc accctgacgc cttccttgcc gaggtcctcc
     6481 agggagttta cctttccagg gttccccgca ggtacaagga tgaccggcac gtgatagcat
     6541 atcgtctcca taccgtcctt gatggatatg cctttctcca gggcatcatt ggtgtacttg
     6601 atgtcaccgg gaatatagac gtcgccctcc tgcctggtgg atatcattcc gaaaagctcg
     6661 ccagaaccgg catagatcat ccttacgggg atccccgcct cctgctcgaa tgcttccttg
     6721 agttcatcca ttggcttcat cagccccgcc ccgcagtaga ccaccagggg ttcctccgtc
     6781 gcgactgcca caggaacaaa agacacagat aaggacaaga ccaggagcag cattgccgtt
     6841 acaagtttcc tcatattccg aacacctcca ttaaaaatgc cccctcctgg ctacaggaag
     6901 agggcacgca taatgacagc ggcccctcct tcccaaacac gggaggcgcc gccgtttcca
     6961 gc
//
LOCUS       DAOPKQ010000055         6917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_11645_length_6917_cov_4.885019, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000055 DAOPKQ010000000
VERSION     DAOPKQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11645_length_6917_cov_4.885019"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="rpsH"
                     /locus_tag="PK146_03060"
     CDS             complement(<1..305)
                     /gene="rpsH"
                     /locus_tag="PK146_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPE91252.1"
                     /translation="MYVNDPVGDMLTRIRNANMVFHETVDMPLSKVKLAIARILKEEG
                     YVKNFKVVNDPKKPYATLRLFLSYGPQKERVIQGLRRISKPGRRIYVQKDELPSVMKG
                     "
     gene            complement(335..520)
                     /locus_tag="PK146_03065"
     CDS             complement(335..520)
                     /locus_tag="PK146_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPE91253.1"
                     /translation="MARKAMRIKATREPKFKVRSYTRCPLCGRVHAYMRQFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            complement(538..1077)
                     /gene="rplE"
                     /locus_tag="PK146_03070"
     CDS             complement(538..1077)
                     /gene="rplE"
                     /locus_tag="PK146_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPE91254.1"
                     /translation="MPRLADLYKKEITGKLQKEFGYTNLMEVPRIQKVVVNIGVGEAK
                     VDYKFMEASIAELRTITGQQPMMKRARKSVAGFKLREGMPVGCSVTLRGARMWEFLDR
                     LISLALPRIKDFQGLSPRGFDGHGNYNLGLKEQLIFPEIDYDKVNRIRGMNISIVSTA
                     RSDEEAMSLLQALGMPFVR"
     gene            complement(1096..1422)
                     /gene="rplX"
                     /locus_tag="PK146_03075"
     CDS             complement(1096..1422)
                     /gene="rplX"
                     /locus_tag="PK146_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPE91255.1"
                     /translation="MSKIRIRKGDRVQVISGKDKGKEGKVLRCFPARDLVLVEGVNVV
                     TKHVKPSAKNPQSGIVKQEAPIYSSKVKLVCTACGAATRVGRAFLEDGRKVRVCKKCG
                     EIIDKV"
     gene            complement(1435..1803)
                     /gene="rplN"
                     /locus_tag="PK146_03080"
     CDS             complement(1435..1803)
                     /gene="rplN"
                     /locus_tag="PK146_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPE91256.1"
                     /translation="MIQLRTVMKVADNSGAKKIMCIQVLGGSRKRYASVGDIVVASVK
                     EAIPNSNAGKGDVVKAVVVRTKKEVRRKDGSYVRFDDNAAVLIDNNGDPRGTRIFGPV
                     ARELREKRFMRIVSLAPEVV"
     gene            complement(1804..2097)
                     /gene="rpsQ"
                     /locus_tag="PK146_03085"
     CDS             complement(1804..2097)
                     /gene="rpsQ"
                     /locus_tag="PK146_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPE91257.1"
                     /translation="MNERTISRKTRVGTVVSDRMEKTVVIKVDRMAKHSLYGKPVLRS
                     KKFMAHDEEETCRIGDKIRIEETRPLSRHKRWKVVEIIERAPVLGGSQDKEAQ"
     gene            complement(2111..2317)
                     /gene="rpmC"
                     /locus_tag="PK146_03090"
     CDS             complement(2111..2317)
                     /gene="rpmC"
                     /locus_tag="PK146_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPE91258.1"
                     /translation="MNARELRELSLDELNDKHRQFKEELFNLRFQHAIGQLGNTGRIG
                     EVKRSIARVLTVMREKEIGLKRPV"
     gene            complement(2320..2742)
                     /gene="rplP"
                     /locus_tag="PK146_03095"
     CDS             complement(2320..2742)
                     /gene="rplP"
                     /locus_tag="PK146_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPE91259.1"
                     /translation="MLMPKRVKYRKPFRRPLSGMAKGGKTVAFGEYGLQALECAWITA
                     RQIEAARVAISRKMKKGGKTWIRIFPDHPFTKKPLETRMGKGKGNPEFWVAPVRRGRI
                     MFEIAGVPREVAEKAFLTASHKLPIKVRLVTREGMGGE"
     gene            complement(2749..3417)
                     /gene="rpsC"
                     /locus_tag="PK146_03100"
     CDS             complement(2749..3417)
                     /gene="rpsC"
                     /locus_tag="PK146_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPE91260.1"
                     /translation="MGQKVHPVGYRLGIVRDWESRWYAGRKDYAEKLHEDLKLREWIV
                     KRWSHAGISKIEIERIGKVIRFTIWTARPGVVIGKGGTEIQKVREEIQELTGNKIMIN
                     VQEIKSPDKEAQVVAEGVASALERRVSFRRAMKQSIFRAMKSGADGIKVQCSGRLGGA
                     EIARTEWYLEGRLPLSTLRADIDYGFAEARTIYGVIGIKVWINKGQAVRPAPAGSQEP
                     VERG"
     gene            complement(3431..3763)
                     /gene="rplV"
                     /locus_tag="PK146_03105"
     CDS             complement(3431..3763)
                     /gene="rplV"
                     /locus_tag="PK146_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPE91261.1"
                     /translation="MEAKAIARQVRLSPTKSRQVLTLIRGKKAGQALDTLRFTPKKAA
                     RVVEKVLKSAIANAEHNYGMDMDRLVVVKAYADQGPSMKRWQPVSMGRVHAYKHRTSH
                     ITVVVAER"
     gene            complement(3776..4057)
                     /gene="rpsS"
                     /locus_tag="PK146_03110"
     CDS             complement(3776..4057)
                     /gene="rpsS"
                     /locus_tag="PK146_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPE91262.1"
                     /translation="MARSTKKGPFVEQKLLRKVEAMNEEGKKRVIKTWSRRSTIVPDM
                     IGHTIAVHNGRTHIPIFISENMVGHKLGEFAPTRKFGGHAGQERSTRVR"
     gene            complement(4076..4903)
                     /gene="rplB"
                     /locus_tag="PK146_03115"
     CDS             complement(4076..4903)
                     /gene="rplB"
                     /locus_tag="PK146_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPE91263.1"
                     /translation="MGIKKYKAYTPGRRTMTTSSFEEITRTEPERSLVESLKKTGGRN
                     NLGRITMRHRGGGSKRLYRIIDFRRNKIGIPGKVASIEYDPNRSARIALINYADGEKR
                     YILAPVGLGVGDTITAGPDSDIRPGNALKLRDIPVGTFIHNIELEPGRGGVLVRSAGT
                     SAQLMAKEGKKAFIRLPSGELRLALLECMATIGQIGNEDHENLTSGKAGKTRWLGRRP
                     HVRGMVMNPVDHPMGGGEGRSKSHKHPVSPWGTPAKGYRTRKRKPSDKFIVRRRYEK"
     gene            complement(4923..5219)
                     /gene="rplW"
                     /locus_tag="PK146_03120"
     CDS             complement(4923..5219)
                     /gene="rplW"
                     /locus_tag="PK146_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPE91264.1"
                     /translation="MKALPQDIILRPVITEKSSRLMEQNKYTFEVHPRANKIEVRDAV
                     SSLFKVKVVKVSTIYVRPKPKRMGAFLGKSRSWKKAIVTVAAGERIEFFEGANA"
     gene            complement(5216..5842)
                     /gene="rplD"
                     /locus_tag="PK146_03125"
     CDS             complement(5216..5842)
                     /gene="rplD"
                     /locus_tag="PK146_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPE91265.1"
                     /translation="MPTVKVINFKGEIIGEESLSDSVFAAPVHVPAMHQVVVAQQANA
                     RQGTHSTKTRGEVRGGGRKPWRQKHTGRARHGSRRSPIWVGGGITHGPHPRDYHQKVN
                     KKVRRLALKSALSLKVSEGSLVLLDSIDIEKPSTKVMASFLSEIDAGKKVLIMVGESS
                     PAIYKSASNIPGTMVLHVDSINVYDILNHNRMVLTMDAARKIEEVYGA"
     gene            complement(5858..6496)
                     /gene="rplC"
                     /locus_tag="PK146_03130"
     CDS             complement(5858..6496)
                     /gene="rplC"
                     /locus_tag="PK146_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPE91266.1"
                     /translation="MGMGILGRKLGMTQIFDEKGQSVPVTVVEAGPCSVVAVKSDAAD
                     GYTALSLSFGDIKPSKLNRPDRGVFEKKGLEPKRWIREFRVEDTDGYEVGSVVSVELF
                     ETGDVVDVTGRSKGKGFAGVIKRHHFGGGPASHGASKFHREPGSAGASSFPGHIFKGK
                     TMPGRMGGERVTVKNLSIAGVDPENNLLLIRGAVPGPRNGLVMIRRQVGAPR"
     gene            complement(6534..6839)
                     /gene="rpsJ"
                     /locus_tag="PK146_03135"
     CDS             complement(6534..6839)
                     /gene="rpsJ"
                     /locus_tag="PK146_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPE91267.1"
                     /translation="MAKKIRIKLKAFDHRALDSSAFQIAETAQRTGAKVSGPIPLPTE
                     VNKYTVLKSPHKDKDAREQFEMRTHKRLIDIIDPTQKTMESLMQLNLPSGVDIQIKL"
BASE COUNT         1199 a         2216 c         1783 g         1719 t
ORIGIN      
        1 cccttcatga cacttgggag ctcgtccttc tgcacataga tcctgcgtcc gggcttgctg
       61 atcctcctga gaccctggat aacgcgctcc ttctgcgggc cgtaggaaag gaaaagcctg
      121 agggtcgcgt aaggcttctt gggatcgttg acgaccttga agttcttcac atagccctct
      181 tccttgagga tcctggcaat agcaagcttg accttgctga ggggcatgtc gaccgtctca
      241 tggaagacca tgttggcgtt ccggatgcgc gtgagcatgt cccctacagg gtcattcaca
      301 tacattgcaa tagcctcctt gaccgcatcc cttgctacca gctggatttg accacaccgg
      361 ggatcttgcc ttcccgggcc agcttccgga agcaacaacg gcacatattg aattgcctca
      421 tgtaggcgtg gacacgacca cagagagggc acctggtgta ggacctgacc ttgaacttgg
      481 gttctctcgt ggccttgatt ctcatagctt ttcgggccat tcgctacatc tcctccctta
      541 ccggacaaaa ggcattccaa gagcctgaag caggctcatt gcctcttcgt ccgatctcgc
      601 cgtggaaacg atggatatgt tcatccccct gatcctgttt accttgtcgt aatcgatctc
      661 ggggaatatc agctgctcct tcagaccaag gttgtagtta ccgtggccgt cgaaacccct
      721 gggcgacagc ccctggaagt ccttgatcct gggcagtgca agggagatga gcctgtcaag
      781 gaactcccac atccttgccc cccggagcgt tacggaacac cccacaggca tcccttcacg
      841 gagcttgaag cccgcaacgg acttcctggc cctcttcatc atgggctgct gccccgtaat
      901 tgttcgaagt tcggcaatgg aggcctccat gaacttgtag tccaccttcg cctctcccac
      961 ccctatgttc actacaacct tctggatccg gggtacctcc atcaggttcg tatacccgaa
     1021 ctccttctgg agcttccccg tgatctcttt cttgtaaaga tctgccagac gcggcatcgt
     1081 caagtcgcct ccccactaca ccttatcgat tatctcgccg cacttcttgc aaacacggac
     1141 cttccgtccg tcctcaagaa aggcccttcc gaccctggtc gcggcgccgc aggccgtaca
     1201 gacgagcttg accttgctgg agtaaatggg agcctcctgc ttgactatcc cgctctgagg
     1261 gttcttggcc gaaggtttca cgtgcttggt gaccacgttg actccctcga ccagaacaag
     1321 atccctggcc gggaaacagc gaaggacctt cccctctttt cccttgtcct tgccggatat
     1381 gacctgtacc cggtctccct ttctgatcct gatcttgctc atcgccaatt cctcctaaac
     1441 aacttccgga gcaagggaga cgatcctcat gaaccgcttc tccctgagct cgcgggccac
     1501 agggccgaag atcctggtac ccctggggtc gccgttgttg tcgatgagca ccgccgcgtt
     1561 gtcatcgaac ctgacgtagg aaccgtcttt ccttcggacc tcttttttgg tcctgacgac
     1621 aacggccttg accacatcgc ccttgccggc gttgctgttg gggatggctt ccttgacgga
     1681 ggccaccact atgtcaccga cagaggcgta acgtttcctg ctccccccca ggacctggat
     1741 gcacattatc ttcttggcgc ctgagttgtc ggcgaccttc ataaccgtcc gcagctggat
     1801 catctactgc gcctccttat cctggctgcc gccgaggact ggtgccctct cgattatctc
     1861 gacaaccttc caccgcttgt gtcggctaag ggggcgagtc tcctcgatcc ttatcttgtc
     1921 cccgatgcgg caggtctctt cctcgtcatg ggccatgaac ttcttgctcc tcaggacggg
     1981 cttgccgtaa agggagtgct tggccatgcg gtcaaccttg atgaccaccg tcttttccat
     2041 acggtcgctg acgacggtac cgactcttgt ctttctggag atggttcttt cattcatccc
     2101 gagttccccc ctacaccggg cgtttgagcc cgatctcctt ctcccgcata acggtaagaa
     2161 ccctggcgat ggatctcttg acctctccga tgcgcccggt gttcccaagc tggcctatgg
     2221 catgctggaa ccggagattg aaaagttctt ccttgaactg tctgtgcttg tcgttcagtt
     2281 catctaagga caattcgcgc agttccctgg cattcatcct tattcaccac ccattccttc
     2341 ccgggttacc aggcggacct tgatcggcag cttgtgagat gccgtgagaa aagccttttc
     2401 tgccacttcc cttgggactc ccgctatttc gaacatgatc ctccctctcc gtacgggggc
     2461 tacccagaac tccggattgc cctttccctt gcccatacgt gtctcaaggg gcttcttggt
     2521 gaaggggtgg tccgggaata tcctgatcca ggtcttgcca cccttcttca tcttacggga
     2581 gatcgccaca cgggcggcct cgatctgccg ggcggttatc caggcacact caagggcctg
     2641 aaggccgtac tctccgaagg caactgtctt gccccccttg gccataccgc tcaaggggcg
     2701 tcggaaaggc ttgcggtatt tgaccctttt cggcataagc atgtccttct accccctctc
     2761 gacaggttcc tggcttccgg caggagcagg cctcacggcc tgacctttgt tgatccagac
     2821 cttgatacct atcacaccgt atattgtccg ggcttcggcg aaaccgtaat cgatatcggc
     2881 cctcagagtg gatagaggca gacgaccctc aaggtaccac tcggtcctgg cgatctcggc
     2941 gccgccaagc cttccgctgc actggacctt gatcccgtcg gcaccggatt tcatggcacg
     3001 gaagatggac tgcttcatgg cccggcggaa gctaacccgc ctctccaggg ccgaagcgac
     3061 cccttcagcc acgacctggg cttccttgtc ggggctcttg atctcctgga cgttgatcat
     3121 gatcttgttg cccgtcaact cctggatctc ctccctgacc ttctggatct ccgtgccgcc
     3181 cttccctatg actacaccgg gtcgcgcggt ccagatcgtg aaccggatga ccttgcctat
     3241 acgttcgatc tctatcttcg agatgcctgc gtgagaccac cttttgacga tccactccct
     3301 gagcttcagg tcctcatgca gtttctccgc gtaatccttg cggcccgcgt accatctcga
     3361 ttcccagtcg cgcactattc caaggcgata cccaacaggg tgtactttct ggcccaacgg
     3421 tcaaccctcc ttagcgctct gcaaccacta cggtgatatg gctggtacga tgcttgtagg
     3481 catgcaccct gcccatcgag accggctgcc agcgcttcat agatggaccc tggtcggcgt
     3541 aggcctttac aacaaccagt cgatccatat ccattccata gttatgctcg gcattggcga
     3601 tcgcgctctt cagaaccttc tcgacgaccc tggccgcctt cttcggggtg aaccggagcg
     3661 tatcaagggc ctgaccggcc ttcttccccc tgatcagcgt aaggacctgc ctcgattttg
     3721 tgggcgaaag tcgcacctgc cttgcaatgg ctttagcttc cataaaaagc ccctcctaac
     3781 ggacgcgggt ggagcgttcc tgcccggcgt gaccgccgaa cttgcgcgtg ggcgcgaact
     3841 ccccaagctt gtgaccaacc atgttctcgc ttataaagat cgggatatgg gtcctgccgt
     3901 tgtgcacggc gatggtgtgc ccgatcatat ccggaacgat ggtggaacgc ctggaccacg
     3961 tcttgatgac tcttttcttg ccttcttcgt tcatggcctc taccttgcgg agaagcttct
     4021 gctcgacaaa gggtcccttc ttcgtggaac gagccatttc cgtccctccc taccactact
     4081 tctcgtaacg gcgccgtacg atgaacttgt ccgacggctt gcgtttacgg gttctgtacc
     4141 ccttggcggg cgtcccccaa ggagataccg gatgcttgtg cgacttgctc cgcccctctc
     4201 caccgcccat ggggtggtct acggggttca tgaccatgcc tctgacgtga ggccgcctgc
     4261 ccagccagcg ggtctttcca gccttgccgc tagtgaggtt ctcatggtcc tcgttgccta
     4321 tctgtccgat agttgccatg cactcaagca gggccagacg gagctcccca ctgggaagcc
     4381 tgatgaaggc cttcttgccc tccttggcca tgagctgtgc cgaagttcct gccgatctga
     4441 ccagtacacc gccgcggccc ggctcgagtt cgatgttgtg gatgaaggtt cccacaggga
     4501 tatccctgag cttgagggca ttcccggggc gaatgtccga atccggtcct gccgtgatgg
     4561 tgtccccgac cccgaggcca acgggtgcga gaatgtatct cttctcgccg tcggcataat
     4621 tgatcagggc gatcctggca gagcggttgg gatcgtactc gatcgatgcg accttaccgg
     4681 ggatccctat cttgttgcgc ctgaagtcga tgatcctgta gagccgcttt gagccgcctc
     4741 cccggtgtct catggtgatc cttccgaggt tgttgcgacc gccggtcttc ttcagagact
     4801 ccacgaggga tctctctggt tcggtcctgg tgatctcctc gaaagaggag gtcgtcatgg
     4861 tcctgcgtcc cggcgtatag gccttgtatt ttttgatacc catcttcttg atacctcccc
     4921 gcctacgcgt tggcgccttc gaagaactcg atgcgctcac cggccgcgac ggtcacgatg
     4981 gctttcttcc aggacctgga cttgcccagg aaggcgccca tcctcttcgg ttttggcctt
     5041 acgtagatag tgctgacctt gaccaccttg accttgaaga gagaggacac ggcgtccctg
     5101 acctcgatct tgttggcccg ggggtggact tcgaaagtat acttgttctg ctccatcagc
     5161 cggctgctct tttcagtgat gaccggcctc agaatgatat cctgcggcag cgccttcatg
     5221 ccccgtacac ctcctcgatc ttcctggcag cgtccattgt caggaccatc ctgttgtggt
     5281 tcaggatgtc gtagacattg atgctgtcca cgtggaggac catcgtcccg gggatgttcg
     5341 aggccgactt gtagatagcc ggactgctct cgcccaccat gatcaggacc ttcttgcccg
     5401 catcgatctc cgacaggaag gatgccatta ccttggtgct gggcttctcg atgtcgatgc
     5461 tgtccagcag tacaaggctg ccctcgctga ctttcagtga aagtgcgctc ttcagggcaa
     5521 gtcttctgac ctttttgttg accttctggt gatagtccct ggggtgtggc ccatgggtta
     5581 tccctccgcc cacccatatg ggggaccttc tgctaccgtg cctggccctt ccggtatgtt
     5641 tctgtctcca tggcttcctg ccgcctccgc ggacctctcc gcgggtcttg gtggagtggg
     5701 tgccctgcct ggcgttggcc tgctgggcca ccaccacctg gtgcatggcc ggaacatgga
     5761 cgggagccgc gaagacggaa tcggaaaggc tctcctcgcc gatgatctct cccttgaaat
     5821 tgattacttt cacagtcggc atagctccgc tgcctcctta ccgtggtgcc ccaacctgcc
     5881 tgcggatcat cacgagcccg ttccttggtc ctggaacggc gcccctgatc agcagaaggt
     5941 tgttttcagg atcaacgccg gctatggaca ggttcttgac cgttacccgt tcgccgccca
     6001 tccttcccgg cattgtcttt cccttgaaga tatgacccgg gaagctgctt gccccggcag
     6061 aacctggctc ccggtgaaac tttgaagcgc cgtgacttgc cggcccgcct ccgaagtgat
     6121 gccgcttgat aacgccggca aatccctttc ccttgctccg tccggtaaca tccaccacgt
     6181 cgccggtctc gaagagttcg acggagacaa cactgcccac ttcgtagccg tcggtgtctt
     6241 ccaccctgaa ctcgcggatc cacctcttgg gctcgagacc cttcttctcg aagacacccc
     6301 tgtcaggcct gttgagcttt gacggcttga tgtccccaaa ggacaggctc agggcggtgt
     6361 aaccgtcggc agcatcactc ttgaccgcta cgacactgca cggacctgcc tcgacaactg
     6421 ttaccgggac tgactgcccc ttttcgtcga agatctgggt cattcccagc ttgcgaccta
     6481 gaattcccat acccatcagt accatctctc tcctttcagc tcattgctcc ccattaaagc
     6541 ttgatctgga tatcaactcc ggaggggaga ttcagctgca taagcgactc catggtcttc
     6601 tgcgtcgggt cgataatgtc gatgagccgc ttatgagtcc tcatttcaaa ctgctccctt
     6661 gcgtccttgt ccttgtgcgg cgacttgagg accgtgtact tgttcacttc cgtaggaagg
     6721 gggataggcc cggaaacctt ggcccccgta cgctgtgccg tctccgctat ctggaacgcg
     6781 gaggagtcca gtgccctgtg gtcgaacgcc ttgagcttga tccggatctt ctttgccact
     6841 gtgacttcct ccccctggca ctagttatcg agtatctctg tgacgacgcc ggcgccgacg
     6901 gtcctgccgc cctcgcg
//
LOCUS       DAOPKQ010000056         6846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_11798_length_6846_cov_4.413194, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000056 DAOPKQ010000000
VERSION     DAOPKQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11798_length_6846_cov_4.413194"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1042)
                     /gene="purM"
                     /locus_tag="PK146_03140"
     CDS             complement(50..1042)
                     /gene="purM"
                     /locus_tag="PK146_03140"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPE91268.1"
                     /translation="MEWTYERAGVSLRRSDAWIDLVRKIASRASSPNVLSGIGGFSGL
                     YRLGGGKVLAACTDGVGTKLEVARMTGSFQGLGQDLVAMNVNDLVTCGARPLFFLDYI
                     ACGHLDGDIFGPVMEGIAESCSSCGCALLGGETAEMPGVYPPGSFDLAGFAVGIVEEA
                     CMIDGGRTAKGDVLLGLASSGLHSNGFSLVRKCLLDGDGSLALDHIVPGDGKPLSKVL
                     MRPTRLYVKAALEAASSGAVTGMAHITGGGLEENISRILPGSLRPEIDFSSWERPPVF
                     GLIGNSGVDEKEMRRVFNLGIGFVLVVRPRDVQIVSSILGNFGETVYVIGEVAS"
     gene            complement(1026..2402)
                     /gene="purF"
                     /locus_tag="PK146_03145"
     CDS             complement(1026..2402)
                     /gene="purF"
                     /locus_tag="PK146_03145"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPE91269.1"
                     /translation="MCGVFGAFCKNGSSVLEDVYLGLYALQHRGQESAGIAWNSENGD
                     EVNSIKGMGLVHIALDQKQLASISASSAIGHVRYSTTGSSGLANAHPITANYSMGSVA
                     IAHNGNITNATGIRNMLESRGAIFQSTTDTETIIHLMAHQSHKPPLDALVDALRRLKG
                     AYSLAVLLGNRLVAARDPWGFRPLVLGRREDTTYVASESCALDIVGATLVRDVEPGEM
                     VVIDETGIKSLRIPVQASRKYSCAFEYVYFARPDSVIDGKSVYEVRKALGCRLAKSSP
                     CPSAEIVTGMPDSGTIAAMGFAECSKQSYETAIVRNRYIGRTFIQPTQRVRTLGVRIK
                     LNPIKTLIDGRELAVVDDSIVRGTTVKRVISMIRQEGAARVHIRIASPPVRFPCYYGI
                     DTPTCEELAAARLDIDTLRSEVGADSLNYLGIGDLLSAIGLPSSDICTACFSGEYLDG
                     GEDNGMDL"
     gene            complement(2402..4558)
                     /gene="purL"
                     /locus_tag="PK146_03150"
     CDS             complement(2402..4558)
                     /gene="purL"
                     /locus_tag="PK146_03150"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPE91270.1"
                     /translation="MRPDHRAAGLSDAEYGEIVRALGREPEHAELMILGVMWSEHCSY
                     KSTRRLLSTLPTNGEAVVQGPGENAGIVDIGEGWGIAFKVESHNHPSAVAPYQGAATG
                     VGGIIRDVLAMGARPVALLDGLCLGEKSCPEADALADGIVKGIAGYGNAVGVPTIGGK
                     TLYDRCYNGNPLVNAMCLGLVPIDGIVSSRTARPGQLAVLIGSRTGRDGIAGAAFASS
                     GLADDTRSSRPQVQIGDPFVEKLLVECCLEIHSKGLIVAMQDMGAAGITSSSSEIAAK
                     SGTGIRIDADLVPLREEMETWEIALSESQERMLLIAEEGALEEIGSIADRWQLDWAVI
                     GKVTDDGLFVMTKGGREVVSLPAEMVGGGCPPVQWPSCKPSAEAEGQERPSDPLDPGG
                     TERCLFTILENPNLAERSRIYRQYDYMVQTNTVVPPGNSVSIFRVRENRSLVAVTMEV
                     DPWKCSLDPFAGAAETFLKALRPLWVSGANHLGMTNCLNFPSPEDPGNHWILERSASG
                     LAAVARDLACPVVSGNVSLYNENPSGKILPSPLVAVVGLQDCNNPPLRDRESIEAGSV
                     FLVGDIKGTLGGSAFSRIITPGAEHKPGCYDPSKEKDFMKRALKTVRKEAASAGRAIA
                     GGGLMTSLAKMCITADIGIGLDSTVLDTTPECLFGEWGPRAVYLVPERSTELFLSCWE
                     GFPVRRIGEITPGGLTLEGHEMLPEETRAGKAGRKL"
     gene            complement(4555..5262)
                     /gene="purQ"
                     /locus_tag="PK146_03155"
     CDS             complement(4555..5262)
                     /gene="purQ"
                     /locus_tag="PK146_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPE91271.1"
                     /translation="MRTSVIVFPGSNCDRDVMHAISASTGTMTDLVWHEHRTLPRDTG
                     LVILPGGFSYGDYLRCGAMAAKSPIIDAVIKHASSGLPVLGICNGFQVLTEIGLLPGA
                     LLANTSLDFICDFCHVRVEREDTPFTGHYRKGEVLRIPIAHYEGRFYLPEEQLRALEE
                     EGLVVFRYCSPEGVTGEDFTPNGSPNGIAGIINRKGNVLGMMPHPERATESLLGSLDG
                     APLWGSVNSWIRGRLGE"
     gene            complement(5259..5516)
                     /gene="purS"
                     /locus_tag="PK146_03160"
     CDS             complement(5259..5516)
                     /gene="purS"
                     /locus_tag="PK146_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPE91272.1"
                     /translation="MTFLAKILVFPREGVLDTQGKAVGKSLNRIGFGSVKEVKVGKYI
                     QVLLEAVTEADAAAAAESMCGELLVNDLIEDHTVQVGPWKP"
     gene            complement(5513..6232)
                     /locus_tag="PK146_03165"
     CDS             complement(5513..6232)
                     /locus_tag="PK146_03165"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPE91273.1"
                     /translation="MQVTDMLYEGKAKKLFITEDPSMLLVEYKDSLTAFNARKKSSLT
                     GKGSLTNRISAELLEFVGTQGVPTHFVRLLDDTCQLVRRVKIIPLELVVRNITAGSIC
                     RRLGVEEGMELKDPLVEFYLKNDDLGDPLVTEDHILSFRWATPSDIYAMKKLALQVNR
                     ILKEFFAGLEIILVDLKLEFGRTPEGNIILADEISPDTCRLWDASTGEPLDKDRFRND
                     LGNVLGSYREIWRRITGREKK"
     gene            6481..6834
                     /locus_tag="PK146_03170"
     CDS             6481..6834
                     /locus_tag="PK146_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HPE91274.1"
                     /translation="MNGFKSPVELAKSACLAAKAKVAWSVPQMLVLGTMAGAYIAFGG
                     WLMTVVTHDLAANMGLGFSRFLGGAIFSVGLMLVVIAGAELFTGNCMMPLSVMAGCTP
                     MSGVIRNWFKAPLLR"
BASE COUNT         1390 a         2027 c         1860 g         1569 t
ORIGIN      
        1 gtggtcatgt tcgaacccct tccggaaatg aggatagcca ttgtgggttt catgatgcga
       61 cctctcctat gacgtatacc gtttccccga aatttccgag gatcgacgag actatctgca
      121 catcccgggg tctgacaacc agaacgaaac ctattccgag gttgaatacc ctcctcatct
      181 ccttctcgtc caccccgctg tttcctatca ggccgaaaac cgggggacgt tcccaggagg
      241 aaaagtcgat ctctggtctc aacgatccag gaagtattct ggagatgttc tcttcaagtc
      301 cgccgcctgt aatgtgtgcc atgcccgtca cggcccctga cgatgccgcc tccagagccg
      361 ccttgacgta caggcgggtt ggtctcatca aaaccttcga gaggggcttc ccgtctccgg
      421 ggacgatatg atccaacgca agggatccat ccccatcaag gaggcacttt ctcaccagcg
      481 agaagccgtt gctgtgcaag cctgagcttg ccagtcccag gagtacgtcc cccttcgcgg
      541 tccggccccc gtcgatcatg caggcttctt ctacgatgcc cacggcgaat ccggcgagat
      601 cgaaggaacc tggagggtag acacccggca tttctgcagt ctcacctccc agaagagcgc
      661 agccgcagga tgaacaggac tcggcgatgc cctccatcac gggtccgaag atatcgccgt
      721 ccaggtggcc acaggcgatg tagtccagga agaagagcgg gcgcgctccg caggtgacca
      781 ggtcgttcac attcatggcc accaggtctt gcccgaggcc ctggaatgat cccgtcattc
      841 gggccacttc cagcttggtc cctacgccat cggtgcaagc ggcaagcact ttgcccccgc
      901 ctaatctgta aagcccggag aagcctccta tccctgaaag gacattcggt gaggatgccc
      961 tggatgcgat ctttcgaaca aggtctatcc aggcgtcgct tctacggagg ctgacaccgg
     1021 ctctttcata agtccattcc attgtcctct cctccgtcaa ggtattctcc gctgaagcag
     1081 gcagtgcaaa tatccgatga aggaagccca atggcggata gaagatctcc gatacccagg
     1141 taattaagtg aatccgcccc aacctctgaa cggagagtat ctatgtcgag tcttgctgcc
     1201 gccagctctt cgcaggttgg agtgtcgatg ccgtagtagc aggggaagcg gacgggggga
     1261 gatgcgatcc ttatgtgcac tctggcagcc ccctcctgcc ttatcatgga aatgaccctt
     1321 ttgaccgtcg tacccctgac tatcgaatcg tccacgacag caagttccct tccgtcgata
     1381 agggtcttga tggggttgag cttgatcctc accccaaggg tacggactct ctgggtaggc
     1441 tggataaagg tccgtccgat gtacctgttc ctgacaatgg cagtttcgta gctctgtttc
     1501 gagcactcgg cgaagcccat tgccgctatg gtcccgctgt cgggcattcc ggtgacgatc
     1561 tctgccgagg ggcagggaga acttttcgca agcctgcacc caagagcctt tctaacttcg
     1621 tagacggatt ttccatcgat cacgctgtcg ggcctggcaa agtagacgta ttcaaaggca
     1681 caggaatact ttcttgaggc ctgaacgggg atcctcagac tcttgatgcc agtttcatcg
     1741 atcacgacca tctctccggg ctccacatcc ctgaccagag ttgctcctac aatgtccagg
     1801 gcgcagctct cggatgccac gtaagtggtg tcctctcgcc ttcccaggac cagggggcgg
     1861 aagccccagg ggtctcgggc cgcgacgagc cggttcccaa gaagtacggc gaggctgtag
     1921 gcgcctttga ggcgcctgag agcgtctacc agcgcatcaa gtggaggttt gtgtgactga
     1981 tgggccatca ggtggatgat ggtttcagtg tctgttgtgg actggaagat ggcgccacgg
     2041 gattcaagca tgtttcgtat tcccgtcgca ttggtaatat tcccgttgtg tgcaatggca
     2101 acagatccca tggagtagtt ggcggtgata gggtgggcgt tggccagtcc ggatgaacca
     2161 gtggtggagt atctgacatg gccgatcgcg gagctggcac tgatggaggc cagctgtttc
     2221 tggtcgaggg caatgtgtac aagccccatc ccctttattg agttgacctc gtccccattc
     2281 tctgaattcc aggctatccc ggccgattcc tggcccctgt gttgcagggc gtaaaggcca
     2341 aggtaaacat cctcaagcac ggagctaccg ttcttgcaga aagctccaaa aaccccgcac
     2401 attataattt cctccctgcc tttccggccc ttgtctcttc cggaagcatc tcatggccct
     2461 caagggtgag accgccgggg gttatttcgc cgatcctcct gacagggaaa ccctcccagc
     2521 atgacaggaa tagttctgtc gatctttcag ggaccaggta tacagcccgg ggtccccatt
     2581 ccccgaagag gcattcggga gtggtatcaa ggacggtact gtcaagtcct attcctatgt
     2641 ccgccgtgat gcacatcttc gccagggagg tcatcaaacc tccccccgct atcgcacggc
     2701 cggcggatgc tgcctccttc cgaacggtct tcagagccct tttcatgaaa tccttttcct
     2761 ttgatgggtc gtagcatcca ggcttatgtt ctgctccagg ggtaatgatc cttgagaagg
     2821 cgctcccccc caaagttccc ttgatatcgc ccaccaagaa caccgatccg gcttctatgg
     2881 actccctgtc gcggagaggt gggttgttac agtcctggag acccacgacg gcaacgagag
     2941 ggctgggaag gatctttccg gaggggtttt cattgtagag gcttacgttc cccgaaacga
     3001 ccgggcaggc cagatcgcgg gcaacggctg ccagaccact tgccgacctt tccaggatcc
     3061 agtgatttcc cgggtcttcc ggcgagggga agttcaggca gttggtcatt cccaggtggt
     3121 ttgccccgct aacccataga ggcctcaggg ccttgaggaa ggtttctgcg gctcctgcaa
     3181 atgggtccag gctgcatttc cagggatcga cctccattgt tactgccaca aggctccggt
     3241 tctccctgac cctgaatatt gaaacgctgt tcccgggtgg gacgaccgta ttggtctgga
     3301 ccatatagtc gtactgtcgg tagatccggg atcgttcggc aaggttgggg ttctcaagga
     3361 tcgtgaaaag acaacgttct gttccccctg gatcaagggg gtctgaaggc ctctcctgtc
     3421 cttctgcctc ggctgagggt ttacaggaag gccattgaac gggggggcag ccgcctccca
     3481 ccatttctgc gggaaggctc accacctccc ttcctccctt ggtcatgaca aagaggccgt
     3541 catcggtcac tttccctatg actgcccagt ccagctgcca tcggtccgcg atcgagccta
     3601 tctcctccag ggctccctcc tcggctatca ggagcatcct ctcctgggac tccgaaaggg
     3661 cgatctccca ggtttccatc tcctccctga gcggcaccag gtcggcgtcg atccgtatgc
     3721 cggtcccact ctttgcggct atctcactcg aggaggaagt gatacccgcc gcccccatgt
     3781 cctgcatggc cacgataagg cctttcgagt ggatctcgag acagcactct accagaagct
     3841 tttcaacaaa ggggtcgcct atctggacct ggggccggct tgacctggta tcgtcggcca
     3901 atccggaaga agcaaaggca gccccggcga tcccatccct tccggtcctc gagcctataa
     3961 gaactgccag ctgacccggt cttgctgtcc tggagctgac gatgccatcg atcggaacaa
     4021 ggcccaggca catcgcattg acgagaggat tgccgttgta gcatcggtcg taaagggttt
     4081 tcccgccaat tgtcggaacg cccactgcat taccgtaacc ggcgattccc ttcacgatcc
     4141 cgtcagcgag ggcatctgcc tcagggcagc ttttctctcc aaggcatagt ccgtccagaa
     4201 gagccaccgg cctggctccc atggccagca catccctgat aatacctccg acaccagttg
     4261 ctgcgccctg gtagggtgcc actgccgaag ggtggttgtg gctctccacc ttgaaggcta
     4321 tcccccagcc ttctccgata tccacgatcc cggcattctc accggggccc tgtacgacgg
     4381 cttctccgtt tgtgggtagc gtagacagca gccgtctcgt ggatttgtaa ctgcagtgtt
     4441 ccgaccacat cacaccaagg atcatgagtt cagcgtgttc aggttctcgc ccgagggccc
     4501 taactatctc tccgtattct gcatcactaa ggccggctgc cctgtgatcg ggtctcattc
     4561 tcccaacctc cccctgatcc agctgttgac cgatccccac aggggagcac cgtccaaaga
     4621 accgagcagg ctctccgtcg cccgttccgg gtgtggcatc atgcccagca cattaccctt
     4681 cctgttgata atgccggcta tcccattggg tgatccatta ggggtaaagt cctctcctgt
     4741 caccccttcg gggctgcagt acctgaacac aacgagtccc tcttcttcca gggccctgag
     4801 ctgctcctcg gggagataga acctcccctc gtagtgggcg atcgggatcc gaagtacctc
     4861 gcccttcctg taatgaccgg taaagggagt gtcttccctt tcaaccctca cgtggcagaa
     4921 gtcgcatatg aaatcaaggg aggtgttggc caggagagct ccggggagaa gccctatctc
     4981 ggtcagtacc tggaatccgt tgcatatgcc caggacgggg aggccagagg aagcatgttt
     5041 aattactgca tcaataatag gggattttgc agccatggca ccgcacctca ggtaatcccc
     5101 gtaggagaac ccgccgggaa ggatcaccag tcctgtatcc cgggggaggg tgcggtgctc
     5161 atgccagacc agatctgtca ttgtcccggt ggatgccgag atggcgtgca tcacatcacg
     5221 gtcgcagttg ctccccggga aaacgatcac cgatgttctc atggtttcca tggtcccacc
     5281 tggacggtat gatcctcgat caggtcgttc accagcagct ctccgcacat ggattcggca
     5341 gctgctgcgg catcggcctc tgtaacggcc tcaagaagca cctggatgta cttgccgacc
     5401 tttacctctt ttacggaccc gaaaccgatc ctgttcaggg actttccgac ggccttcccc
     5461 tgggtgtcga gaacaccctc ccgggggaat acaagtatct ttgcgagaaa agtcatttct
     5521 tctccctccc tgtaattctt ctccagatct cacgatagga tcccagcacg ttcccgaggt
     5581 cgttcctgaa cctgtccttg tccagcggtt caccggtcga ggcatcccat agcctgcaag
     5641 tgtcaggcga gatctcgtca gccagtatga tgttcccctc aggggtcctc ccgaactcga
     5701 gcttgagatc caccaggatg atctcaagac cggcgaagaa ctcctttaga atacggttta
     5761 cctggagggc cagtttcttc atggcgtata tatccgaagg agtggcccag cggaaggaca
     5821 ggatgtgatc ctcggtaaca agaggatcac cgagatcgtc gttcttgagg tagaactcga
     5881 ccagcgggtc cttcagttcc attccttcct ccactcccag ccttcgacag atagatccgg
     5941 cggttatgtt ccttaccacc agttccaggg ggatgatctt gactctgcgt accagctggc
     6001 aggtatcgtc cagcagtctt acgaagtggg tgggaacccc ctgggtgcca acgaactcaa
     6061 gaagctcggc gcttatcctg ttggtaaggc ttccctttcc ggtgagactg gattttttcc
     6121 gggcgttgaa agcagtcagg gagtccttgt attcaaccag aagcatcgaa gggtcttccg
     6181 ttatgaacag tttcttggcc tttccctcat agagcatatc cgttacctgc aaagaacctt
     6241 ccctcctccc catatatact tacattattt gtgttatagc tataaatgtc aatgttctat
     6301 tcgagggagc atgtgaaagg gctggtggaa agaaggggtc tattagtgac acaaataaca
     6361 ttccatgcta gaataccagg cgatcagggg cttagccccg gtctgccccc cggaatacac
     6421 gggaatatcc cagagtctgt aaccgggtgg gactattacc agtgaggagg aatcacctta
     6481 atgaacggtt tcaagtctcc tgtagaactg gccaagtccg catgccttgc ggcgaaggcg
     6541 aaggtggcct ggagtgttcc ccagatgctt gtactgggta ccatggcagg tgcgtacata
     6601 gccttcgggg ggtggctgat gaccgtggtg acccatgacc ttgccgcgaa catgggcctc
     6661 ggtttctccc gattcctcgg cggggcgata ttcagtgtgg gactgatgct cgtcgtcatc
     6721 gcaggagcgg agctttttac cgggaactgc atgatgcccc tcagcgtaat ggcaggctgt
     6781 acccccatga gcggggtgat aaggaactgg ttcaaagctc cgctcctgcg atgacgacga
     6841 gcatca
//
LOCUS       DAOPKQ010000057         6753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_11987_length_6753_cov_5.860107, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000057 DAOPKQ010000000
VERSION     DAOPKQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11987_length_6753_cov_5.860107"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PK146_03175"
     CDS             complement(<1..547)
                     /locus_tag="PK146_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPE91275.1"
                     /translation="MAQEFAEQEYFVLHLLSISEEPLGAGSIREELARSGMDLSEASV
                     GRLLRGLDGRGFTTRIGFQGRLLTPAGEERLSDLQSEREQIVSTGAFLEAVRSREKEM
                     LLEILVARRAIEREMAALAAEMRTKKDLVALRKILDRQNTLLARGEPMASTDPEFHEE
                     VARISGNKVLQSAIEIIRRSGE"
     gene            complement(1059..2762)
                     /locus_tag="PK146_03180"
     CDS             complement(1059..2762)
                     /locus_tag="PK146_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPE91276.1"
                     /translation="MNAARALLEMLQRYQVEYVFGLPGETTLGLYDEWKRFEGVTHVM
                     ARDERHAVFMADGYARVSGRPGVCEGPSVGATHMVPGLVEAMKSCIPLIVMTTDIPLG
                     MEKHNMLTGFDQTSLFTPFVKESLTAHRGDEVPFLVRRAFRVATSGCPGPVHLRLPID
                     VLAQEISPPLPLAQERFSICPGTRPLPGGGETAEAAKGLAYCKKGVMVCGQGVLTSRA
                     WDEIERVAERFGLATATTINGKGSISELHPLSVGVTGSRGSTDLSNSFIEEADLLFYV
                     GCSTDSVGTDGWKLPKETGMTRFIHLNISERELGNSFGDGLLLHGDVKSTLQAILEAA
                     NELELPFKEGREEIAGRRGTRPGPPSTSGDRLDPRLATEMITGAMPEGSVIVADPGMS
                     AVYPAAFYKLPKAGRYFVTNYAMGALGYGLPAAIGATYGVPENVTVLNFVGDGSFGFT
                     SGELETTSRLGRDLKVILFNNGSFGWIRATNLFSFGAPHFATDFSQVDYMALAKSYGM
                     PAFRASSESELNAALSGLFAHRGPALLELVVPPEDECVPPVPEWGIKSRQEGLECHYW
                     R"
     gene            complement(2800..3711)
                     /locus_tag="PK146_03185"
     CDS             complement(2800..3711)
                     /locus_tag="PK146_03185"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HPE91277.1"
                     /translation="MEKSPVRVAVVGYGHIAREAVKAINAAQDMEIAGIVIRDPGKVD
                     EISRQSGLPVFLGSEKLDGADVAILAIASRAVPEVAPSYLERGICTVDCFDIHGEALM
                     LLRERLHRSALKGNSAAMTGAGWDPGTDSIFRGVFEVIAPRGITYTSFGPGVSMGHTV
                     AVKQIQGVRDALSLTLPAGQGMHRRAVYVELDEGTSLENVRKAVLCDSYFCNDETTVE
                     EVGDVKSMVDLGHGVRMERKGVSACADNQRMAVEMTLTNPSATAQVMVSAARGIVLQP
                     PGCYTMLEIPVADFLPGDREANIRRLV"
     gene            complement(3986..4594)
                     /locus_tag="PK146_03190"
     CDS             complement(3986..4594)
                     /locus_tag="PK146_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE91278.1"
                     /translation="MTLETRREPAKTRQPVMELIARRWSPKAFGPEVPSGNELASMFE
                     AGRWAPSCFNNQPWRYLVTTRNDPGAFSRALACLTEKNASWVRNAPLLAFQVMRETFE
                     YNGKPNPWSGFDCGLAMAQLIIQAEFMGWSVHVMAGFSRDRVRETFGVPEGFKPLVAF
                     VIGREGDPHSLPPHFMEEETAPRERKELEETVFSDSWGKSLF"
     gene            complement(4599..5609)
                     /locus_tag="PK146_03195"
     CDS             complement(4599..5609)
                     /locus_tag="PK146_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPE91279.1"
                     /translation="MKAIVMNSTGTSEVLSFGEHPLPEPAPGEVLVRLEFAAVNHLDI
                     WVRIGNVPVKLPIIPGSEGAGVVEATGQGEEAFSPGQEVVVTPWIYPEEHEQLPLNAY
                     ARVLGVARNGCYARYASVPASCLRPAPPGLSLEEAAGVTLVGATAYHMTVTRGGLKPG
                     ERVFVMGATGGVGIAAIQIARASGAVVFAATRDPEKRERLREIGAHEVFDTAGDYPAK
                     VREATGGVGVDLVIETVGKATWDRSLSLLRPGGRLSFCGSTSGSDYTINLQRIYRNEI
                     SLLGCYGAPPEETGLLFEMSARGSVRPVIDSILPLKEAPRAQERMERSDHFGKILLRM
                     ES"
     gene            5741..>6753
                     /locus_tag="PK146_03200"
     CDS             5741..>6753
                     /locus_tag="PK146_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE91280.1"
                     /translation="MKYDVVIVGAGPSGLFAARELAAGGKRVAIIDKGKEIRDRSCPL
                     KEGRSATCVHCKPCNVICGWGGAGSYSDGKLTLTPEFGGNLEQYIGRRELMELIDSVD
                     ATFVSHGASREVFSPDPGFARDIAIRASTAGLRIIPAAIRHMGTDRSKEILQSMFLEI
                     SRSCDVMMGTVVESVVIKDGRAAGVRLADGTVIESVAVLLAPGREGSLWMEKTVSALG
                     LRIKSLPVDIGVRVEIPAGIAEAVTDQFYEVKAILDTPTFDDQVRTFCMCPHGEVTTE
                     YLEQHDILTVNGHSNRDGNGRTGNTNFAILVSTDFTEPFKDPNGYGSHIARLANMLGG
                     GVLV"
BASE COUNT         1312 a         2076 c         1953 g         1412 t
ORIGIN      
        1 cttcgccgct tctgcggatg atctctatgg ccgactgcag gaccttgttg cccgagatgc
       61 gggccacctc ctcgtggaac tcagggtccg tcgatgccat gggctcgcct ctggccagca
      121 gtgtgttctg cctgtccagg atcttcctta gcgcgaccag gtcctttttg gtcctcatct
      181 cggcggcgag ggcagccatc tctcgctcga tggcccttct ggccaccagg atctccagca
      241 gcatctcctt ctcacgggat ctcacggcct ccaggaaggc tcccgtggag acgatctgct
      301 ctctttccga ctggaggtcc gaaagacgtt cctccccggc gggtgtgaga agccgaccct
      361 ggaagccgat cctggtggtg aagccccggc cgtccaggcc gcggagcagg cgacccaccg
      421 aggcctcgct gaggtccatg ccgctccttg ccagctcttc cctgatggag cccgcaccta
      481 ggggctcctc cgatatggag agaaggtgga ggacgaagta ctcctgttct gcgaactcct
      541 gagccatgag aaccctcccg gggaggccgc tcatcagtga tgaccggcaa cgctcccatc
      601 agtttatcgc cagaattcct tttgtcaaat gcctgaataa ccgtgacttg aaactcgtgg
      661 cgcgaaaagc ggcaacaaaa agcaaaggca taaactgtca acggtagagg cggaaaaacg
      721 gaaaccgtgg atcgtgaacc gaaaaagcgt tagaagtgag acgcaaaacg tgagacgaaa
      781 gggttaaact caaagggatc aacgaccctc ggtcgcttgc tctccgggtt ttaacctcca
      841 aaaagatcca ggttttcatc tctctcactt ttctcccgca gaaactccgg gacctcatct
      901 gcgttctaac ctacgctggc ggtgccccag ctaggtcact gcgtcttcga cctctgtggt
      961 gaaagggttc aggtttggct ctggcttttc ccgttcactg ttgactgttt accgttcacg
     1021 cctttgatct tgcaccctgt ttcctgccgc cttgtttctt acctccagta atgacactcc
     1081 agcccttcct gccgggactt gatcccccac tcaggtacgg gggggacgca ctcgtcctcg
     1141 ggggggacca caagttccag cagcgcaggg ccccggtggg cgaaaagtcc ggaaagagcc
     1201 gcgttgagct cgctctccga tgaggcccgg aaggcaggca tgccgtagct tttcgcaagg
     1261 gccatgtaat cgacctggct gaagtcagtg gcgaagtggg gggcgccgaa actgaagagg
     1321 tttgtggccc ttatccatcc aaaactgccg ttgttgaaaa ggatgacctt gaggtccctt
     1381 ccgagcctgg aggtcgtctc cagctcgcct gaggtgaagc caaagctgcc gtctccgacg
     1441 aagttcagga ccgtcacatt ttcgggcacg ccgtaggtgg ctccgatggc ggccggtaga
     1501 ccgtacccca gcgctcccat ggcgtagttg gtgacaaagt agcgccccgc ttttggaagc
     1561 ttgtagaagg cagcaggata gactgcgctc atgccggggt cggccactat caccgaacct
     1621 tcaggcattg cccctgtgat catttcagta gccagcctgg gatcgaggcg gtctcccgag
     1681 gttgatggtg ggccaggcct ggttcctctc ctgccggcga tctcttccct gccttctttg
     1741 aagggcagtt ccagttcatt ggcggcctcc aggatggcct gcagggtgct cttcacatcc
     1801 ccgtggagta gcaggccgtc cccgaaagag ttgccaagct ctctctcgga gatgttcaaa
     1861 tggatgaaac gggtcatccc cgtctccttt gggagcttcc acccgtcggt cccaaccgag
     1921 tcagtgctgc agcctacata gaacaggagg tccgcctcct cgatgaagga gttggagaga
     1981 tccgtgctcc ccctggagcc ggtcactccc acggaaagag ggtggagctc agaaatgctc
     2041 cccttcccgt tgatggtcgt agccgtggcg aggccgaacc tctccgcgac cctctcgatc
     2101 tcatcccagg ccctggaggt tagcaccccc tggccgcaga ccatgacccc cttcttgcag
     2161 tatgcaaggc cttttgccgc ctcggcggtc tccccccccc caggcagggg tctcgtaccg
     2221 ggacagatac tgaaacgctc ctgcgccagc ggcagcggcg gggagatctc ctgggccagc
     2281 acatcaatgg gcagcctcag gtgcaccggg ccggggcacc ccgatgtggc caccctgaag
     2341 gctctccgga caaggaaggg tacctcgtcg cccctgtggg cggtgaggct ctcctttacg
     2401 aagggcgtga acaaagatgt ctggtcgaac ccggtcagca tgttgtgttt ctccataccg
     2461 agggggatat cggtggtcat gactatcagg ggaatgcagg atttcatggc ttccactaag
     2521 cccgggacca tgtgggtggc ccctacgctg ggtccctcgc agacgccggg gcgtccgctg
     2581 acacgggcgt atccgtcggc catgaaaacg gcgtggcgct cgtcgcgggc catgacgtgg
     2641 gtcacccctt cgaacctctt ccattcgtcg taaaggccca gggtggtctc acccgggagg
     2701 ccgaaaacgt attccacctg gtagcgttga agcatctcaa gaagagcgcg ggctgcgttc
     2761 attgggaaca tcctctcttt ctcgtttttc attccattct catacaaggc gcctgatgtt
     2821 ggcctccctg tcgccgggga gaaagtcggc gacgggtatc tccagcatgg tgtagcaccc
     2881 gggaggctgg agcactattc cccgggccgc cgataccata acctgggctg tggcgctggg
     2941 gttggtgagg gtcatctcca cggccatacg ctggttgtcg gcacaagcgg agaccccctt
     3001 gcgttccatt cttacgccgt gacccaggtc gaccatgctc ttgacgtccc cgacctcttc
     3061 cacagtcgtc tcgtcgttac agaagtagct gtcgcagagc actgccttgc ggacattctc
     3121 aagggaggtt ccttcgtcca gctccacgta gaccgctctc cggtgcattc cctgtccggc
     3181 aggcagcgta agggagaggg catccctgac cccctggatc tgttttacag caacagtgtg
     3241 gcccatgctg accccggggc cgaagcttgt gtaggtgatc ccccgagggg cgatgacctc
     3301 gaagacccca cggaagatgg aatccgtacc cgggtcccac ccggcccctg tcatcgccgc
     3361 agagttcccc ttaagcgccg atcggtgaag tctctctctg agaagcatga gggcctcgcc
     3421 gtgaatgtcg aaacagtcca cggtgcagat acctctctcc aggtaggagg gggccacctc
     3481 cgggacggcg cggctggcga tggccagaat ggccacgtct gctccgtcca gcttttccga
     3541 ccccagaaag acaggaaggc cgctctggcg agagatctcg tccacttttc cggggtcccg
     3601 gatcacgatt cccgcgatct ccatgtcctg ggctgcattg atcgccttga cagcctctct
     3661 tgcaatgtgt ccgtagccga cgacggccac ccttaccggg gatttctcca aatcgtccaa
     3721 ccccttcatt catcaaaaat gagcaatcaa aatatatacc agtccctcgg atccgtcaat
     3781 ccgaaagaga tccgtctaac tgcttaacgg taattttttc agtgaatagc tggtttcagc
     3841 agattgggag cagcggtgtc cagtggaagg atccttccgt tcctctacga aaagaaagag
     3901 aggatagcca cttttgagtg atcattaatg atgagtggct acccccgttc aagtgaaacc
     3961 ctctgtccca ggctctgaag aaaagtcaga agaggctctt cccccaagaa tcggagaaga
     4021 ccgtctcctc aagctccttt ctctcccttg gtgccgtttc ctcttccatg aagtggggcg
     4081 gcagggaatg gggatctccc tctcggccta tgacgaaggc aacaaggggt ttgaacccct
     4141 cgggtacccc gaaagtctcc cggaccctgt cgcggctgaa tcccgccatc acgtggaccg
     4201 accatcccat gaactcggcc tgaattatca gctgggccat ggccagaccg cagtcgaagc
     4261 ccgaccacgg gttgggcttc ccgttgtact cgaaggtctc cctcatgacc tggaaggcaa
     4321 gcagtggtgc gttccggacc catgatgcgt tcttctccgt caggcaggcc agggcccggg
     4381 aaaaggcccc cgggtcgttt ctggtggtca ccaggtacct ccagggctga ttgttgaagc
     4441 acgacggggc ccatctgcca gcctcgaaca tcgatgccag ttcgttcccg gaggggacct
     4501 cgggtccgaa ggccttgggg ctccaccttc tggctataag ctccatcacc ggttggcgtg
     4561 tttttgcggg ttctctcctg gtctccagtg tcattatctc agctctccat tctcagaagg
     4621 atcttgccga agtgatcgct gcgctccatc ctctcctggg cccggggtgc ctctttaagg
     4681 gggaggatgc tgtcgatgac agggcgaacg ctccctctgg ctgacatctc gaagaggagg
     4741 cccgtctcct ccggaggcgc tccgtagcaa ccaaggaggg atatctcgtt ccggtagatc
     4801 ctctgaaggt tgatggtata gtccgaaccc gaggtggagc cgcagaagga aagcctcccc
     4861 cccggtcgaa gcagcgacag gctcctgtcc caggttgctt ttccgacggt ctctatcacc
     4921 aggtcaaccc ccacgccccc cgtcgcctcc ctgactttgg caggatagtc acccgcagta
     4981 tcgaagacct cgtgggcgcc tatctccctg aggcgttccc ttttttcggg atcgcgagtc
     5041 gcggcgaaga caacggcccc tgaagccctg gcgatctgga tcgccgctat tcccacgcct
     5101 cccgtggccc ccatcacgaa gaccctttct ccgggcttca gccctcctcg cgttactgtc
     5161 atgtggtagg cagtggcccc cactagcgtt acacctgcgg cctcctcaag ggaaagaccc
     5221 ggcggggcag gtctgaggca cgaagcggga accgatgcgt accgggcgta acagccgttc
     5281 ctggcaaccc ccagcaccct tgcgtaagcg ttcaggggca gttgttcgtg ctcctctgga
     5341 tagatccagg gggtgacaac aacctcctga cccggcgaga aggcttcttc tccctgtcct
     5401 gtggcctcga ccaccccggc tccctcggag ccggggatga tggggagttt cacgggtacg
     5461 ttccctatcc tgacccagat gtcgaggtgg tttaccgcgg cgaattccag cctgaccagg
     5521 acctctcccg gtgccggttc gggcaatgga tgctctccga atgagagaac ctcagacgta
     5581 ccggtggagt tcatcactat tgccttcatg tctgtcactc ccttcaggag gatatctggt
     5641 ctttacctgt tcattttaac aaaacaggct ccctccggcc ctgcttaagg caggggttat
     5701 aatgatccag gaaatgcagt gttgaacaag aggtgagatc atgaaatacg acgtggtgat
     5761 cgtaggggca ggaccctcgg gtcttttcgc ggcccgtgaa ctggccgcag gcgggaaaag
     5821 agtcgccatc atagacaagg gcaaggagat cagagaccga agctgccccc tcaaggaggg
     5881 caggagcgct acctgtgtcc actgcaagcc ctgcaacgtc atctgcggct ggggaggcgc
     5941 gggttcctac agcgacggca aacttaccct tacacccgag ttcggcggaa acctggagca
     6001 gtacatcggg cgcagagagc tgatggagct gatagattcc gtggatgcaa ccttcgtgag
     6061 ccacggcgcc agcagggagg tgttctcccc cgaccccgga ttcgccaggg acatagccat
     6121 acgggccagc accgctggcc ttaggatcat accggccgca ataagacata tgggtacgga
     6181 tcggtccaag gagatcctgc agagcatgtt cctggagata tcccgatcct gtgatgtcat
     6241 gatgggaacc gtggttgagt cggtagtcat caaggacgga agggctgccg gggtaaggct
     6301 tgcggacggt accgtcatag agtccgtagc agtgctcctt gcaccgggtc gggagggttc
     6361 cttatggatg gagaagactg tcagtgccct gggccttagg ataaagtccc ttcctgtgga
     6421 tatcggggtg cgtgttgaga tacccgcagg aatagccgaa gccgtcacgg accagttcta
     6481 cgaggtcaag gctatcctcg ataccccgac cttcgacgac caggtcagga ccttttgcat
     6541 gtgcccccac ggtgaggtga ccaccgagta cctggagcag catgacatcc tcaccgtcaa
     6601 cgggcacagc aaccgcgacg gcaacggccg tacgggcaac accaacttcg ctatcctcgt
     6661 ctccactgac ttcaccgaac ccttcaagga ccccaacggt tacgggagcc atatcgcaag
     6721 gcttgccaac atgctcggcg gaggggtgct ggt
//
LOCUS       DAOPKQ010000058         6749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_11999_length_6749_cov_3.687929, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000058 DAOPKQ010000000
VERSION     DAOPKQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11999_length_6749_cov_3.687929"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK146_03205"
     CDS             <1..407
                     /locus_tag="PK146_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE91281.1"
                     /translation="LRVGYAIAPKTVVEAYDKLHPVFEVSRVGQYAAHAALLETDFIR
                     NIRLKTVSERTRLVRSLSETGIRTEPTRTNFILLKHRQSDEIYERLLHEGVIVRKASD
                     LGLKGYLRVTVGLPEENEFFVSSLKKVLVQLG"
     gene            400..1242
                     /locus_tag="PK146_03210"
     CDS             400..1242
                     /locus_tag="PK146_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91282.1"
                     /translation="MAEKKDSPLLDDVRVFAFVGPAGTGKSQRAQLVALELGVDLIID
                     DGLLIRKGQIVRGKSAKTEKNQVRAIRRALFEYHDHRRAVVEYLSLSQPCSVMLIATS
                     SGMVTRILANLGLPEPEKIIHIEEVSSPEEISRAKKERSFKGQHVIPVSHIQVRKHFA
                     GKLVGRLKVLFDNSDSMEGEKTIVRPPFKFFGEVSIDPEAINQMARFIAERTSQVSSV
                     VSVQTRPANGGIGIQMELGYRPGQRSMVLLGDSLRERTSLAVAGITGLDVHRVDISFV
                     GVEI"
     gene            1242..1853
                     /locus_tag="PK146_03215"
     CDS             1242..1853
                     /locus_tag="PK146_03215"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPE91283.1"
                     /translation="MTSKSGIPLGRRDEAWERLVLAAQRCGKCPLAPGRKNVVFGDGD
                     RSTRLLFIGEAPGAEEDEQGIPFVGKAGQLLTRILEAAGIDRKDIYITNVVKCRPPGN
                     RVPSIEEMHSCEDILKSQVALINPSIIVCLGATPTKWILKTGPGESISKIRGKWFDWK
                     GIRVMPMFHPSFLLRNQSKKKGSPRELTWIDIQEVRDRWLECK"
     gene            1844..2545
                     /gene="uppS"
                     /locus_tag="PK146_03220"
     CDS             1844..2545
                     /gene="uppS"
                     /locus_tag="PK146_03220"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPE91284.1"
                     /translation="MQIETGKKVPSHVAVIMDGNGRWALQRGLPRLAGHREGAKAVER
                     VIRAAAASGIEYLSLFAFSTENWRRPKAEIEGLMSLLGSYLRSKKEELVKNRIRMVFS
                     GRIEQLPQDLRQEIDLFERATAPGDLLTVAVCLNYGGRQEIVDAVNGLLKRGFNGEIT
                     EDLITANLYRPEIPDPDLLIRTSGELRISNFLLWGSAYAELYFTDTLWPDFGGEELQK
                     AIDQYSGRERRYGAV"
     gene            2532..3353
                     /locus_tag="PK146_03225"
     CDS             2532..3353
                     /locus_tag="PK146_03225"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPE91285.1"
                     /translation="MERSRPPARDLAVRSASGLALGLAILGGIYLGNPYWFALCLVIC
                     MVSLGEFYRMISRKGHVSRVVGFGAGIFSLFAAVYSNDPAVLLPLLAAAPILVLFVEV
                     ARKHILGESQGITSSGGTAAGILYIVLPWSFMVVLRDHMWGSYLLVALFACTWSCDVF
                     AYLVGSRWGNTLLCPAVSPRKTVEGFLGGLTGSLLCGGALAFYWGMAPLPIIMIALFC
                     GTFGQIGDLAESLLKREADIKDSGDLIPGHGGMLDRFDSILVNSVLVFFVFGVLW"
     gene            3356..4498
                     /gene="dxr"
                     /locus_tag="PK146_03230"
     CDS             3356..4498
                     /gene="dxr"
                     /locus_tag="PK146_03230"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPE91286.1"
                     /translation="MKTRNIAVIGATGSVGGSVLDVCSMFPGTFRVWALAAKRNVDDL
                     LELGKRFGSDLLVLADPDPSEGLHRVPGREFSFLVGEEALLAMIEDPRCEEVVFASSG
                     TGALPALVMAIQKGKRIYLANKEIIVAAGEWIMPLARNRIIPLDSEHNAIWQCLRGEE
                     PSAVRKILLTASGGPFLHTPEEELREVTFEMAGRHPVWPMGKKISIDSATLMNKGIEM
                     IEAHHLFGQPVGKIGAVIHPQALVHGMVEFVDGSVKMLYSRADMRLAVLAALGVEDRL
                     ENLDPNLSPPKLPGISLEFIEPDEKKFPCLALARQACLLGGPYPPLLVGADEGAVEMF
                     MEGRIPFTGIASKIESVLSSYSGSEPRSWEDALELVVWARERVLHG"
     gene            4541..5584
                     /locus_tag="PK146_03235"
     CDS             4541..5584
                     /locus_tag="PK146_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPE91287.1"
                     /translation="MGLSVLAFLLVIAICVVSHEFGHLLAARMSGVQVHEFSFGMGPV
                     LFSSRKDDMVWSIRTVPIGGFVRLAGMGEEQDEEKVLPGRSFQERSPWERLMILAGGS
                     VSNIALAILFTAILLWGHGVLDLGSTRIGEIMEGYPAEKIGLLPGDTIKEISGIRVEN
                     WGLLSSTIREIAPEGPIMLTFERNGVVHVLEVNIPLDPGQKVPLLGIRPSMTKYPFPR
                     AVAGSLGYIWDFSIEIVKGIWIWATGRGQVDITGPVGIASMAGEAARDGFWTFLAFLS
                     MINLHLGILNLLPFPALDGGRILLVTGEIVTGKKLPPRLENLVHMAGFILLILLLIFV
                     TWKDLARLLFPGR"
     gene            5610..6674
                     /gene="ispG"
                     /locus_tag="PK146_03240"
     CDS             5610..6674
                     /gene="ispG"
                     /locus_tag="PK146_03240"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HPE91288.1"
                     /translation="MQTRNVKVGQLGIGNGFPVRVESMLRTPLHDLQGCLEEVLSLAD
                     EGCELVRVAFPEMVLSGNLSRLVEGSPTEIMADIHFDHRLAIEAIRSGCRSVRINPGN
                     MGGPGRIRELSSAVKDSGVVVRIGANSGSLSRSRLEQAKGDAGRALFDAVQEQEDALH
                     CCGVEDIIVSAKSSSLDETLRANSLIASSSCHPIHIGLTEAGPGQRGIIKSSVAIGSL
                     LGSGIGDTVRISLTGTSLEEVVVAKRVLQSLGIRRFEAELVSCPACGRRRADVGSLVR
                     IIEPLMKNLRKDLKVAVMGCEVNGPGEAADSDIGIAGTPSGIVLFLGGKPIGHCSLEE
                     LSTQFLKVCRESGLCRGSQD"
BASE COUNT         1483 a         1749 c         2057 g         1460 t
ORIGIN      
        1 ctctgcgtgt agggtatgcg atcgcaccta agaccgtggt ggaagcctac gacaagctcc
       61 accctgtttt cgaggtgagc agggtggggc agtatgcagc ccatgccgcc cttcttgaga
      121 ccgatttcat caggaatata aggctgaaga cagtttcgga gaggaccagg cttgtaaggt
      181 ccctttcgga gacagggatc cggacagaac ccacccggac gaacttcatc ctcctgaaac
      241 atcggcagag cgacgagatc tatgaacgtc tccttcatga aggggtgata gtcagaaagg
      301 ccagcgacct cgggctgaag ggatacctca gggtcaccgt cggattgcct gaggagaacg
      361 aattcttcgt ctcatcactg aaaaaggtgc tggtgcagct tggctgagaa aaaggatagc
      421 ccccttctcg atgacgtcag ggtctttgcc tttgtcgggc ccgcaggaac aggaaagagc
      481 cagagagcgc agctggtggc gctggaactt ggcgtggatc tcattatcga cgacggactt
      541 ctcataagga agggacagat cgtcagggga aagagcgcaa agaccgaaaa gaaccaggtc
      601 agggcgataa ggagagccct tttcgagtat cacgatcacc gaagggccgt tgtggaatac
      661 ctctccttgt cgcagccctg ttcggtgatg ttgatcgcca cctcatcggg aatggtgacc
      721 cgtatcctgg caaacctggg ccttccggaa cccgagaaga taatccacat agaggaagtc
      781 tcctcgccag aggagatatc ccgggccaag aaggagaggt cctttaaagg tcagcacgtc
      841 atacctgtct ctcacatcca ggtcagaaag cactttgccg gaaaactggt cgggaggctg
      901 aaggtccttt tcgacaactc cgacagcatg gagggagaga agaccatcgt ccggcccccc
      961 ttcaagttct tcggagaggt cagcatcgat cctgaggcta taaaccagat ggcacgtttc
     1021 atcgccgaga ggacctccca ggtatcctcg gttgtttcgg tccagaccag acccgccaac
     1081 ggagggatag ggatacagat ggaactggga tacaggccag ggcagagatc gatggtcctc
     1141 ctcggggaca gcctgcgcga gaggacctcc ctggctgtgg cggggatcac cggactggac
     1201 gttcatcgtg tcgatatatc ttttgtgggg gttgagatct aatgacttcg aagagcggta
     1261 tccccctcgg tagaagggat gaagcttggg agagacttgt tcttgcagcg cagaggtgcg
     1321 ggaagtgtcc ccttgccccg ggacggaaga acgtggtctt cggcgacggg gaccggtcca
     1381 cgcgccttct cttcattggc gaggcgcccg gtgcagagga ggatgaacag ggtatcccct
     1441 ttgttggaaa ggccggtcag ctcctgacaa ggatcctcga ggcggcggga atcgacagga
     1501 aggatatcta catcaccaac gtggtaaaat gcagaccccc ggggaaccgg gtcccctcca
     1561 ttgaggagat gcactcctgc gaggatattc tgaagagcca ggtggcactt atcaacccgt
     1621 cgatcatcgt ctgtctcggg gcaacaccga cgaagtggat cctcaagacg gggccgggag
     1681 aaagcatctc caagatcagg ggaaagtggt tcgactggaa gggcatcagg gtaatgccca
     1741 tgttccatcc cagttttctt ctgagaaacc agtcgaagaa gaagggaagc cccagggagc
     1801 tgacatggat agatatccag gaggttcggg accgttggct ggaatgcaaa tagagaccgg
     1861 gaagaaggtg ccgtcccacg tggcggtaat aatggatggg aacggaagat gggcccttca
     1921 aagaggcctc ccccgcctgg caggacatcg tgagggtgcc aaggccgtgg agagggtgat
     1981 aagggctgcg gcagcctcgg gtatagaata cctttccctt ttcgctttct ccacggagaa
     2041 ctggaggcgt ccgaaggccg agatagaagg tctcatgtca ctgctcggct cctacctcag
     2101 atccaaaaag gaagaactcg tcaagaacag gataaggatg gtcttctcgg gaaggatcga
     2161 acagctcccc caagacctca gacaggagat cgaccttttc gagagagcca ctgccccggg
     2221 agacctgctg accgtagcgg tctgcctgaa ctacggcggc aggcaggaga tcgtcgacgc
     2281 cgtgaacggc ctgctcaaga gaggtttcaa cggggagatc acagaggacc tgataaccgc
     2341 caacctttac aggcccgaga tccccgatcc cgatctgcta ataaggacca gcggggagtt
     2401 gaggataagt aatttcctgc tatgggggtc ggcctacgcc gagctgtatt ttactgacac
     2461 tctctggccc gatttcggcg gtgaggagct gcagaaagcc atcgatcaat actccggcag
     2521 ggagagaaga tatggagcgg tctagacccc cagccaggga tcttgccgta cgttccgcaa
     2581 gtggcctcgc cctcgggctg gccatactgg gaggcatcta tcttgggaac ccctactggt
     2641 tcgccctgtg cctggtcatc tgcatggtct ctctgggtga gttctacaga atgataagca
     2701 gaaagggaca tgtttcgagg gtcgtcggct tcggtgccgg tatcttctct ctctttgccg
     2761 cggtctactc caatgatcct gcagtactgc tccccctcct ggctgcagcc cctatactgg
     2821 tcctctttgt agaggtggcc aggaagcaca ttcttggcga gagccaggga ataaccagct
     2881 ccgggggtac cgctgccggg atcctttata tcgttcttcc ctggtccttt atggtagtac
     2941 tccgggacca catgtggggg agctaccttc tggtagccct ttttgcatgt acctggtcgt
     3001 gcgatgtttt cgcctacctc gtgggctcca ggtggggtaa cacgctactc tgtccggccg
     3061 ttagtcccag gaagaccgtt gagggttttc tgggcggtct gacgggaagc cttctctgcg
     3121 gaggggcact ggctttttac tggggtatgg ctcctcttcc gatcatcatg atcgccctct
     3181 tttgcggaac cttcggccag ataggggacc tggccgaatc ccttctcaag agagaggcag
     3241 atatcaagga ttccggtgac cttatccctg gccacggggg aatgcttgac aggtttgaca
     3301 gcatcctcgt caattccgtt ctggtttttt tcgtcttcgg agtgctctgg tagccatgaa
     3361 gacaaggaat attgccgtta tcggtgccac cggaagcgtt ggaggatcgg tacttgatgt
     3421 ctgctccatg ttcccgggta ctttccgggt ctgggccctt gccgcgaaga gaaatgtgga
     3481 cgatctgctg gagctgggca agaggtttgg aagcgatctt cttgtacttg ccgaccccga
     3541 tccatcggaa gggcttcacc gggtaccggg aagggagttc tccttcctgg ttggggaaga
     3601 agcgcttctg gccatgatag aagacccgcg ctgtgaggag gtggtttttg cctcgtccgg
     3661 aaccggtgct cttccagctc ttgttatggc aatccagaag ggcaagagga tctacctggc
     3721 caacaaggag atcatagtag cggcagggga gtggataatg cctctggccc ggaaccggat
     3781 catcccactg gacagtgagc ataacgcgat atggcaatgc ctcagggggg aagaaccttc
     3841 agcagtacga aagatccttc ttacagcatc gggcgggcct ttcctccata ccccggaaga
     3901 agaactccgg gaagttactt tcgagatggc agggagacac cctgtatggc cgatggggaa
     3961 gaagatatcg atcgacagcg ccacccttat gaacaaggga attgagatga tagaggcaca
     4021 ccaccttttc ggacagcccg tcgggaagat cggagccgtg atccaccccc aggctcttgt
     4081 ccacggcatg gtagaattcg ttgacggttc tgtcaagatg ctctactcaa gggctgacat
     4141 gagacttgct gtcctggcgg ccctcggagt ggaggatcgc ctggaaaacc tcgatccgaa
     4201 cctctcgcct ccgaaactgc cgggtatttc ccttgagttc atcgaacccg atgaaaagaa
     4261 gttcccctgc cttgccctgg caagacaggc ctgtctgctt ggaggtcctt accccccgct
     4321 tcttgtgggg gctgacgagg gggcggtgga aatgtttatg gaaggccgta tcccctttac
     4381 aggcatagcc tcgaagatag aaagtgtgtt atcctcctat tccggaagcg aaccccgatc
     4441 ctgggaagac gcactggagc ttgtcgtatg ggcaagggaa agggttctcc acgggtaggc
     4501 gccgggtatt tcaggaccac ggactgaaag ggatgaagag atgggtctga gtgtgctggc
     4561 tttccttctg gttatagcga tctgcgtggt ttcccacgaa tttggacacc tgctggccgc
     4621 caggatgtcg ggcgtccagg tccacgagtt ctctttcgga atgggaccgg tcctcttcag
     4681 cagcaggaaa gacgatatgg tgtggtccat acggactgta ccgataggcg gtttcgtacg
     4741 tctggccggg atgggagagg agcaggacga ggagaaggtc cttcccggca ggtcctttca
     4801 ggagagatcc ccctgggaac gcctgatgat cctggccgga ggatcggtct ccaatatagc
     4861 actggcgatc ctcttcacag cgatcctcct gtgggggcat ggagttctgg accttggcag
     4921 tacccgtatc ggcgagatca tggagggata ccctgccgag aagatcggac ttctcccggg
     4981 ggataccatt aaagaaatat cagggatccg cgtagagaac tggggtttgc tttcatcgac
     5041 cataagggag atagctccgg aagggcccat catgctgact tttgaacgta acggagtggt
     5101 acatgttctc gaggtcaaca taccccttga tcccgggcaa aaggtcccac ttctcggtat
     5161 aagaccttcc atgacgaagt accccttccc aagagccgta gcggggtctc tgggctatat
     5221 atgggatttc agcatagaga tagtcaaagg tatctggatc tgggcgacgg gccgcggtca
     5281 ggtagatatc accgggccgg tcgggatagc ctcgatggca ggggaggctg caagggacgg
     5341 gttctggacc ttccttgcct ttctctctat gatcaaccta catttgggca tccttaacct
     5401 gcttcccttc ccggccctcg acggagggag gatcctgctt gtcacaggcg agatagtaac
     5461 aggaaagaaa ctcccgccca ggctggagaa tctggtccac atggctggtt tcatccttct
     5521 gatccttctg ctgatattcg tcacatggaa ggaccttgca aggctccttt tcccaggcag
     5581 gtagacccga tacggaggca ttgaaccaga tgcagaccag aaatgtaaag gtgggacagc
     5641 tcggtatcgg caacggtttt cctgtcaggg ttgaaagcat gctcaggacc cctctccatg
     5701 atctgcaggg gtgtcttgaa gaggttctct ccctggctga cgaaggttgc gaactggtca
     5761 gggttgcctt cccggaaatg gttctctccg gaaacctttc gaggctggtc gagggatcac
     5821 cgacagagat catggctgat atccatttcg accaccggct ggccatcgag gcgatcagat
     5881 ccggttgccg gtctgtcagg atcaaccccg gcaacatggg aggacccggg aggatcaggg
     5941 agttgagctc ggcggttaag gattcgggag tagtggtacg gataggcgcc aactccggat
     6001 ccctttccag gtcccggctg gaacaggcca aaggtgatgc aggaagggcg ctcttcgatg
     6061 ccgtccagga acaggaagat gcccttcatt gctgcggggt agaggacatc atcgtctctg
     6121 ccaaatcttc atcccttgat gagaccctga gagccaattc actgatcgct tccagctcct
     6181 gtcaccctat ccatatcgga ttgaccgagg caggacccgg ccagagaggg atcatcaaga
     6241 gctctgtcgc gatcggttcg ctgctgggga gcgggatagg cgatacggtg aggatcagtc
     6301 tcacgggcac ctcactggag gaggtagtag tggccaagag ggtccttcag tcccttggga
     6361 taaggcgttt cgaagcggag ctggtttcat gcccggcctg tggccggaga agagcggacg
     6421 ttggttccct ggtcaggatc atcgagccgc tgatgaaaaa cctcaggaag gacctgaagg
     6481 tcgcggtcat gggttgcgag gtgaatggtc caggagaggc tgcagattcc gatatcggta
     6541 ttgccggcac tccttctggt atagtccttt tcctgggagg caagcctata gggcactgca
     6601 gcctggagga gctttcaacg cagttcctga aggtctgcag ggagagcggt ctttgccgag
     6661 gatcccaaga ttgacacccc cccttctggc gatataatga tatgcgcttt ttcgggaatg
     6721 accacctgat aggaggcttg ccttgacat
//
LOCUS       DAOPKQ010000059         6657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_12255_length_6657_cov_5.323993, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000059 DAOPKQ010000000
VERSION     DAOPKQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12255_length_6657_cov_5.323993"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PK146_03245"
     CDS             complement(<1..819)
                     /locus_tag="PK146_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPE91289.1"
                     /translation="MNILSQEGLFRTISGKRWIVVLMAAWLLVKVCPALALETPGTAL
                     RFVGGDFGTPMVEGSVAREMETSFRERFFSPWNRDHLEVQPEDSLWAWEHWKEVEPWG
                     ENLRPHSREWMDFLLDNCSMESWGSVNRRALACVDTDLRTLPTARPFFRDPSLAGEGF
                     PFDYLQNSRVKAGEPLFVGHFSADGAWAFVETGYASGWVDTRDLAFADEDLVRQWQDM
                     PLAFITDDGVTISDNSGIYRFTARIGTVLPIIRSGNGGPVTVTHDEVTRYFMTIP"
     gene            853..1356
                     /locus_tag="PK146_03250"
     CDS             853..1356
                     /locus_tag="PK146_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPE91290.1"
                     /translation="MGLENQIIILGKVALAILLGGLIGLERETIGKPAGMRTHMLIAG
                     ASTLFAALGSVIINYFGEVTCGTDLVRSDPIRVIQAIALGISFIGAGTIMQHPERRKV
                     IYLTSAASILFTAGVGIAVAVDQFFLAVGCVAIVWIVNRGLRYIDKKVLKRRTPLSRS
                     RKSDLNE"
     gene            complement(1371..2381)
                     /locus_tag="PK146_03255"
     CDS             complement(1371..2381)
                     /locus_tag="PK146_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE91291.1"
                     /translation="MSQEMEGFSRMAGDLAKVLFESRTTGVCILRDGMILAANPLVGE
                     MTEKPDEEIKGKPWLELASREDLPFLGSGRGQGTSRETTPFPFRLASPRGETRWVVGT
                     LAPLPSPAGSDELLLLSDVSRYERIHDDASVREQDTLAMTEGTLRAIEKIVEMKDPFI
                     AGHQRRVARLSYAISRRLGLSESSSRVLRMAALVHDVGKVSIPIQVLAKPMHLSEDEY
                     SIVRMHPLVGYEILKDIDHLAFAAEIILQHHERVDGSGYPSGLTGDRMLPEARIISVA
                     DAMEAMISRRPHRPALGLEEALGERDRGRGLEYDPDVVDSCLDLFKEPGFTLESRSEG
                     DF"
     gene            2500..3054
                     /locus_tag="PK146_03260"
     CDS             2500..3054
                     /locus_tag="PK146_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPE91292.1"
                     /translation="MNDSEMAERFLEVIEDTILPMTKEAVREGHNIFGGAVLKSDDLS
                     LVVAGTNRRGIDPTLHGEMVTIHNFFMGPNHPDPAETLFLATHEPCSMCLSALAWSGF
                     RRIYFLFGYDETRDDFRMGDDLRILAEVFSTTVPTRSNSFFELVPIRSMLLDLQERGP
                     FTARISKIKKSYKALSDLVLASAG"
     gene            complement(3051..3560)
                     /locus_tag="PK146_03265"
     CDS             complement(3051..3560)
                     /locus_tag="PK146_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPE91293.1"
                     /translation="MNKAERVVDFAMKMELEARDFYLDSMERVSRADTRSLLATLAEW
                     EKGHYDFLKQHKDSLEGTGKWGEVAPPAESLEEAVEIVMEKKTGTAVEPPLGETSSDF
                     SVLRMAMAIETDFRNFYGNAARTMNDPEGRKILEMLSEWEGGHQEYINEQYRVLHREF
                     MNEMGFEPF"
     gene            complement(3697..>4653)
                     /gene="selD"
                     /locus_tag="PK146_03270"
     CDS             complement(3697..>4653)
                     /gene="selD"
                     /locus_tag="PK146_03270"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPE91294.1"
                     /translation="KDLPGQEDGFLLTSWAHGEDAAVWMVDDKRAGIFTLDFITPVVD
                     DPFVFGQIAAANALSDVFAMGGRPIVALNIVGFPVNCQPLEMLTQILKGGQERVTAAG
                     AFLAGGHSVEDEEPKYGLAVYGEVEAKNIWRVTGARPGDSLILTKPLGTGVVITAFQA
                     EMDDPGEIDAAVESMRSLNDLPLNLPQQVYREVHACTDVTGFSLAGHLLDMLSAGGLG
                     VRLFAEDLPILPGALEKAGMGLVPAGSYRNRELYSPRVDGLEGISTSLSDLVFDAQTS
                     GGLLLAVSEEIASRFLEVCRSSGFPHSAIIGSFTEGEGGISF"
     assembly_gap    4654..4753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4941..6038)
                     /gene="yedE"
                     /locus_tag="PK146_03275"
     CDS             complement(4941..6038)
                     /gene="yedE"
                     /locus_tag="PK146_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HPE91295.1"
                     /translation="MFSSLNRLLMSRMGPAVTGAVVGVLAPLLSFWGNPANMGICVAC
                     FSRDIAGALGMHRAAVVQYIRPELSGFILGSLAAALVYKEFKPRAGSSPIIRFFLGVF
                     AMIGALVFLGCPWRAYIRLSAGDFTAIAGIAGLTVGVIIGIYFLWTGFSLGRSYKAKT
                     PMGMIMPALAIVLLSLLIMAPTFGPEGTGPIFFSETGPGSQAAPIAIALGVGLIVGWL
                     AQRSRFCTVGAIRDLVMLRDGHLFKGVLAFIFAAFVMNLLLGQFRLGMEGQPVAHSNN
                     LWNFMGMVLSGLAFTLAGGCPGRQFIMSGEGDGDSGIFILGMIVGAGIAHTFSMASSP
                     AGPGPFGPGATIVGLIFCLAIGFLMREKASL"
BASE COUNT         1468 a         2004 c         1739 g         1346 t
ORIGIN      
        1 gggaatggtc atgaagtagc gggtgacttc gtcatgggtc accgtcaccg gacctccgtt
       61 accgctcctg atgatcggaa ggaccgtacc gatcctggcg gtgaacctgt agattccgct
      121 gttgtcactg atggtaacac cgtcgtctgt tatgaaggca aggggcatgt cctgccactg
      181 cctgaccagg tcttcatcag cgaaggccag atccctggta tccacccaac ccgaagcata
      241 gcccgtctct acgaaggccc aggctccgtc ggcagagaag tgccccacga agaggggctc
      301 tccggccttg accctggagt tctggaggta gtcgaagggg aaaccctcac ccgccagaga
      361 ggggtccctg aagaagggcc tcgccgttgg cagggtcctg agatcggtat cgacacaggc
      421 cagggccctc ctgttcaccg agccccagct ctccatggag cagttgtcca ggaggaagtc
      481 catccactct ctgctgtggg gtctaaggtt ctctccccag ggttcgacct ccttccaatg
      541 ctcccacgcc caaagggaat cttccggctg gacctcaaga tggtccctgt tccagggcga
      601 gaagaacctc tccctgaaac tggtctccat ctcccttgct acagatcctt cgaccatggg
      661 ggtcccgaag tctccgccca caaagcgaag ggccgtcccg ggcgtctcaa gggccagggc
      721 agggcagacc ttgacgagca gccacgccgc cataaggacc acgatccatc tttttcccga
      781 aatggtacga aagagtcctt cttgggataa tatgttcatc agcaacctct tccattacaa
      841 ggaggtaaaa ccatgggtct cgagaaccag atcatcatac tgggcaaggt cgccctcgcg
      901 atcctcctgg gaggcctgat aggccttgag agggagacga tagggaagcc tgcgggcatg
      961 aggacccata tgcttatcgc cggtgcctct accctctttg cagccctggg gagcgtgatc
     1021 ataaactatt tcggggaggt cacctgcggc actgaccttg tcaggagcga tcccatccgt
     1081 gtgatccagg ccatcgccct agggataagc ttcatcggtg ccgggacgat catgcagcac
     1141 cccgagaggc gaaaggtgat ctaccttacc tctgcggcct cgatcctctt caccgccggt
     1201 gtcggaattg ccgtggccgt cgaccagttc tttttggcgg tcgggtgtgt agccattgta
     1261 tggatcgtca accgcgggct ccgttatatc gacaagaagg tactgaagag aagaacgccc
     1321 ctttcccgct cccgcaaaag cgacctcaat gaataacggc ctttggaggg tcagaagtct
     1381 ccttcagacc tgctctcgag ggtgaaaccc ggctccttga agaggtccag gcaggaatcg
     1441 accacgtccg ggtcgtactc caggccgcgc cccctatctc tctcccccag ggcttcctcg
     1501 agtcccagcg ccggccggtg gggcctcctg gagatcatgg cctccatggc atcggccact
     1561 gagattatcc tcgcctccgg tagcatcctg tccccagtca gtcccgacgg gtaaccggag
     1621 ccgtcgaccc tctcgtggtg ctgaaggatg atctccgccg caaaggccag gtggtcaata
     1681 tccttgagga tctcgtaacc cacaagtggg tgcatcctga ctatgctgta ctcgtcctcg
     1741 gataggtgca tgggtttcgc caggacctgg atcgggatgg agacctttcc cacatcatgg
     1801 acaagggcgg ccatcctgag gaccctggag gaagactctg acaggccaag gcgccttgat
     1861 atggcatagg ataaccttgc cacccttctc tggtggcccg caatgaaagg gtctttcatc
     1921 tccacaatct tctctatggc ccggagggtt ccctcggtca tagccaaggt atcctgctcc
     1981 ctgacactgg catcgtcatg gatcctttcg taccttgata cgtcggacag caggagcagc
     2041 tcgtcggatc ctgccgggga aggaaggggc gccagggtcc cgaccaccca gcgggtctct
     2101 ccccggggag aggcgagcct gaaagggaag ggcgtggtct cacgggaggt cccctgtccg
     2161 cgaccggacc ctaaaaaagg cagatcctcc ctggaggcca gttcaagcca gggttttccc
     2221 ttgatctcct cgtccggctt ctccgtcatc tcgcccacca gagggttggc tgccaggatc
     2281 atgccatccc tcaggatgca gaccccggtg gtcctgcttt cgaaaaggac ctttgccaga
     2341 tccccggcca tccgggaaaa gccctccatc tcctggctca ctctcttcac ctccaaaacc
     2401 ctgtatacct ctttctgcgt tattctacca caccattaaa atgaacctca ggcttcacaa
     2461 aggaccgggt tctgatatct tatcagaggg ggtgatggct tgaacgactc cgaaatggcg
     2521 gaaaggttcc tggaggtcat tgaagatacc attctgccca tgaccaaaga agccgtaaga
     2581 gaaggccaca acatcttcgg gggtgccgtt ctgaagtctg atgacctctc cctggtggtg
     2641 gccggtacca acaggagggg catcgacccg accctccacg gcgaaatggt gaccatccac
     2701 aactttttca tggggccaaa ccaccctgat cctgccgaaa cgctcttcct ggcaacccac
     2761 gaaccctgtt ccatgtgcct gtccgctctg gcctggtcgg ggttccggag gatatacttc
     2821 ctcttcggct acgacgagac ccgggacgac ttcaggatgg gcgacgacct gaggatcctg
     2881 gccgaggtct tctcaactac agtacccacg aggagcaact cctttttcga acttgtcccg
     2941 atccggagca tgctccttga ccttcaggaa aggggaccct tcactgcaag gatctcaaag
     3001 ataaagaaga gctacaaggc cctttccgac ctggtccttg cctctgcagg ctagaagggc
     3061 tcgaagccca tctcgttcat gaactcccgg tgcagaaccc tgtactgttc atttatgtac
     3121 tcctggtgcc caccctccca ctcggagagc atctcgagga tcttgcggcc ctcagggtcg
     3181 ttcatggtcc gggcagcatt cccgtaaaaa ttcctgaaat ccgtctcgat agccatggcc
     3241 atccttagaa cactgaaatc ggacgaggtc tcacctagtg gcggttctac cgctgtcccg
     3301 gtttttttct ccattacgat ctccacggct tcttccaggc tctcggcagg gggagccact
     3361 tcgccccatt tccccgttcc ctcgagactg tccttgtgtt gtttcaggaa atcgtagtgc
     3421 cccttctccc actccgccag ggtagccagc aggctcctgg tgtcagccct gcttactctc
     3481 tccatggagt cgaggtagaa atccctggcc tcaagttcca ttttcatggc gaaatccact
     3541 actcgttccg ccttgttcat caaacacacc tccgaaaaat gtttttaagt catttttatt
     3601 ttatcaaaat agccaaaaaa cgaatcatca taatggctgg ataatggata caaaaaagcg
     3661 gcccccgaaa aagagggccg ctatatccaa cgccggtcag aacgatatcc cgccttcccc
     3721 ttcggtaaag cttcctatta tcgccgagtg ggggaaacct gagcttctgc agacctccag
     3781 aaatctgctg gcgatctctt cgctgacagc gagcagaaga ccaccggagg tctgggcgtc
     3841 aaaaaccaga tctgaaaggg atgtcgagat cccttccagc ccgtccaccc tcggtgaata
     3901 gagctccctg ttccggtaag agcccgcggg taccagaccc atacccgcct tttcgagagc
     3961 accgggcaat atcggcagat cttccgcaaa aagccttact cccagtccgc cggcggaaag
     4021 catatccagg agatgccccg caaggctgaa tccagtaacg tcggtacagg catgaacttc
     4081 gcggtatacc tgctggggca gattcagggg aaggtcgttg agagaccgca tggattccac
     4141 cgctgcgtct atctcgcccg ggtcgtccat ttcagcctgg aaggcggtga tcaccacccc
     4201 ggttcccagg ggtttcgtca gtatcagcga atccccgggg cgcgcaccgg taaccctcca
     4261 gatgtttttg gcctcgacct caccgtagac ggcaagacca tacttcggtt cctcgtcctc
     4321 gacgctgtgc ccacccgcca ggaaagcacc cgctgcggtc acccgctcct gccccccctt
     4381 gagtatctgc gtcagcattt ccaggggctg gcagtttaca ggaaaaccca cgatgttcaa
     4441 ggcaacaatg ggccgaccac ccatggcgaa gacgtcgctc agagcgttgg cagctgcaat
     4501 ctgtccgaaa acaaacgggt cgtccacgac gggagtgatg aagtccaggg tgaatatccc
     4561 cgcccgcttg tcgtccacca tccagacggc ggcgtcctcc ccatgtgccc agctggtcag
     4621 caggaaccca tcctcctggc cgggaaggtc tttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnngagacct cccaaccctg gttgcgggcc aggcgggata cgttctccct
     4801 tgaggtaact gtatccacca gaacctctat tttacccccg gatcgtctgc ccatggcttt
     4861 ttttgtctcg accaccggct ggggacatga gagcccgcgc gcatccaccg taattgttcc
     4921 gctcattgtc gaaaacctcc ctataagctt gctttctccc tcatcaggaa accgatggcg
     4981 agacagaaga tcaaccccac aattgtagcg ccgggaccga aaggtccggg acccgccggt
     5041 gaactggcca tgctgaaggt atgggcgata ccggcgccga cgatcattcc cagaataaag
     5101 atacctgagt cgccgtcacc ctcgccgctc atgatgaact gccttcccgg acacccaccg
     5161 gccaaggtaa aggccagccc cgagagtacc atgcccatga agttccacag gttattggaa
     5221 tgagccacgg gctgcccctc cattcccagc cggaactggc ccaggagaag attcatgacg
     5281 aatgctgcaa aaataaaagc caggaccccc ttgaagagat gaccgtcccg gagcatcacc
     5341 aggtcgcgta tagcccctac ggtgcagaag cggcttcgct gggcaagcca gcccacgata
     5401 aggccaaccc ccagggctat cgcaatggga gcagcctggg aacctggacc cgtttccgag
     5461 aagaatatcg ggccagtccc ctccggaccg aatgtcgggg ccatgatcag caaagacaat
     5521 aggactatcg caagtgcggg catgatcatc cccattggtg tctttgcctt gtagcttctc
     5581 ccaagactga aacctgtcca aaggaaatag atgcctatga tcacacccac cgtgagccct
     5641 gcaatacctg caatggccgt gaagtccccg gcgctgagac ggatgtaggc cctccatggg
     5701 caacccagga aaacgagggc tcctatcatg gcaaagaccc ccaggaagaa acggatgatc
     5761 ggcgaggatc cggcccttgg cttgaactcc ttgtagacaa gagcggccgc tagcgatccg
     5821 aggatgaaac ccgagagctc cggcctgatg tactgaacca cagccgccct gtgcatccca
     5881 agggccccgg cgatatccct gctgaaacag gcgacgcata tccccatgtt ggccggattc
     5941 ccccagaaac tgagaagggg cgccaagaca cccaccacag cccccgtaac tgcgggtccc
     6001 attcgtgaca tcaatagtct gttaagactt gaaaacattc tcttcaactc cacctttcca
     6061 atatgctgat ggttccatgc tccggttgat ctcgtcagga tcgaacttcc ggcagacgaa
     6121 gagcaaggca catggatgaa cgcgcacgct gaaaggcggc cacgctgccg aggctggtga
     6181 acagttgaaa ctttttcagc cggagaggaa aggatggaaa aaccttgaag gaaaaatcag
     6241 gaacggttcc ggcggccttc cggcattaaa gaacaaattc ggcagcaatc aatactcgca
     6301 tggggcgaga cgtgcgtcct ccatgaggtg aaaaagtgcg ggacccgcac ccggactgct
     6361 gctcatcgac tccggttggc catggcggaa cctcctttct tgctggggtt tttcgttttc
     6421 actgaaagga tagcagaaaa acaagacctt gaatagcgaa taacgaacag gtgaattcca
     6481 tggaatgacc gcttgcaggc tgtcccggca gtctacctgt tgcctcatat aagaatctaa
     6541 gaaaatagca ggaaaatagt agaggtgtca gacctagaaa atagtagggg tgtcagacct
     6601 tgacgggctg ttgcccaatt gagtcccgca ggtttaaaaa aggcagatca ggcaaga
//
LOCUS       DAOPKQ010000060         6647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_12280_length_6647_cov_4.270631, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000060 DAOPKQ010000000
VERSION     DAOPKQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12280_length_6647_cov_4.270631"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /gene="secD"
                     /locus_tag="PK146_03280"
     CDS             complement(<1..1328)
                     /gene="secD"
                     /locus_tag="PK146_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPE91296.1"
                     /translation="MLKKDRWRLVLVAVVVVAALLSVFPIGGRIRLGLDLKGGAHILL
                     QAQGTSENPLTDDSIERLLAVLRNRIDQYGVTEPVIQREGSDRVIVDLPGVADPEAAL
                     ELIGKTALLEFRHVHESTGTVPPGPQRPNYDTQEEFLEAQARWEAVREQFDRAAEAMR
                     LRADEDPDMIMSRSDEGEAYLLGRAYVSGNDLEDAKTDFDNLGRPVVTLKFSTQGARL
                     FDEATAANVGRQIAICLDGKVISAPVVQERISGGNAQISGRFSTAEAQRLSIMLRAGA
                     LPVAVEILENRSVGPTLGSDSIRSGLRAGLIGALLVLVFMLLYYRTLGIAADIALAVC
                     MLLVFAGLISLKATLTLPGIAGIILTLGMAVDGNILIYERMKEEVAGGKTPYAAVDSG
                     FRKAMTTILDANITTLIAAGVLFYFGTGPVRGFAVTLSIGIVASVFSAVIV"
     gene            complement(1498..1791)
                     /gene="yajC"
                     /locus_tag="PK146_03285"
     CDS             complement(1498..1791)
                     /gene="yajC"
                     /locus_tag="PK146_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPE91297.1"
                     /translation="MGMLLPLGVFVVIFYFLIIRPQKKRQRKHEEMLASIRRGDQIVT
                     AGGFFGVVREVKEDSFIVEIADGVKARILKASIQMKKHEGEAVEPSVSEDLAG"
     gene            complement(1920..2546)
                     /locus_tag="PK146_03290"
     CDS             complement(1920..2546)
                     /locus_tag="PK146_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPE91298.1"
                     /translation="MKIAEPTVERLVLYYRLLSQLHNEERRVVSSQEIGEMLGLKASQ
                     VRKDLSYFGEIGKRGVGYHVDRLYQHISNILSPNRVWKIALVGVGRLGEALLGHKALR
                     SDKFKLEALFDNDPSKVGQVICGIPCYDSEEMTEIIRRKEIEVLILTVPAGAAQISAD
                     RAVAAGTVKGVLTFATTAISVPEDVLVYRVDISVELEKLLFFLKGSVA"
     gene            complement(2644..2916)
                     /locus_tag="PK146_03295"
     CDS             complement(2644..2916)
                     /locus_tag="PK146_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPE91299.1"
                     /translation="MRIDRFLKLSRIVKRRSVAQEMITHGAVRVGGTEVRPSKDLRSG
                     DVVEVAFPRKIITLRVISVDESLLKRGGECFEVTDERNVPGDQRPW"
     gene            complement(3006..4136)
                     /gene="rpoD"
                     /locus_tag="PK146_03300"
     CDS             complement(3006..4136)
                     /gene="rpoD"
                     /locus_tag="PK146_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPE91300.1"
                     /translation="MESHDTGDETVASPREVARYIESFKELLHMGREKGFVTYDDIEK
                     HIPGEMMNEETLDSLYFNLNELGIDLVEMEKTRGDLRDEDSAPAAEYNEEMGVLEDLP
                     LSDPVRMYLREIGKIALLKPEEEVELAKLVEAGDPVGKSKLVEANLRLVVSIAKKYIG
                     RGMLFLDLIQEGNLGLIRAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPV
                     HMVETINKLIRVSRQLVQRLGREPTAEEIAAEMAIGSERVEEIQRIAQEPVSLETPIG
                     EEEDSQLSDFLEDKNLPSPEDAAAVQLLREQLEGMLEELTEREKEVLRLRFGLEDGHP
                     YTLEEVGKRFGVTRERIRQIEAKALRKLRHPSRSKKLRDFLD"
     gene            complement(4163..5917)
                     /gene="dnaG"
                     /locus_tag="PK146_03305"
     CDS             complement(4163..5917)
                     /gene="dnaG"
                     /locus_tag="PK146_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPE91301.1"
                     /translation="MDRDAVRNIKDRIDIVEFIGETVQLRRAGKSFKGLCPFHSEKTP
                     SFHVSSERQTYHCFGCGRGGDIFSFVMEKEGMTFPEALTMLADRAGVRLEPYGGRRES
                     TSGVTGAMEAALAFFRRSLTGPGGESARKYLERRGIGSSDSAAFEIGWAPPSWDLLRK
                     DLETRGIPTGEAMAAGLLAESSGRTYDRFRGRIMFPIRDVQGRLVAFGGRLVDGEGAK
                     YINSPESELYSKRRILYLLNSARTSIRERGRSILVEGYMDAIKLHMAGFTETVASLGT
                     SLTGEQANLLKRFGDQCLICYDADTAGQEATLRGMYVLQKSGLDIRVVVLPSGKDPDD
                     IVSGAEGPERFAGLTRRSEPLPLFHIRVKRADLADEARRTAARREIIEGLAGLPPLEA
                     NRHISQVAQGLGILLPEMIDLLRSARRSAAKVGEKGISAPENVYVSRENDRTREDRPP
                     DPWEAALCYLLWTDERKRSAVDPPYVLSLLSEDGAKTVALSILSGDSIEELRSRWAEA
                     DDPYPMRLISTGWAHCERNGESGEIWEIVVSALESKRSRDKFASLKEKHARGQATPEE
                     MKEYLELARKLKGGTGKR"
     gene            complement(6042..>6647)
                     /locus_tag="PK146_03310"
     CDS             complement(6042..>6647)
                     /locus_tag="PK146_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPE91302.1"
                     /translation="LTALALFAAYATAVGLTVIDINNPKVMVGLFIGGMLPFVFSAMT
                     IQAVGRAASQMIDEVRRQFREIPGIMEGTAEPEYERCVDISTGASLKEMVVPGFLAVI
                     SPVVVGLVLGAEALGGLLGGSIVTGVMMAIFMSNAGGAWDNAKKFIEEGNYGGKGSEP
                     HGAAVVGDTVGDPFKDTAGPSLNILIKLMSVVALVLAPLFI"
BASE COUNT         1245 a         2148 c         1776 g         1478 t
ORIGIN      
        1 actattacgg cactgaagac gctggcaacg atccctatcg acagggtcac ggcgaatcct
       61 cttacgggtc cggtgccgaa gtagaagagc acgcccgccg cgataagggt cgtaatattg
      121 gcgtcaagga tggtggtcat ggccttgcgg aatcctgagt ccacagccgc atagggtgtc
      181 ttcccccctg caacctcctc cttcattctt tcgtatataa ggatgttccc gtctacagcc
      241 atgcccaggg tcagaatgat cccggcgatg cccggaaggg tgagagtggc cttaagactg
      301 atgagccccg cgaatacaag cagcatgcag accgccaggg caatgtcggc ggctattcca
      361 agagtgcgat agtaaaggag catgaatacc agaacgagaa gagcaccaat aaggcccgcc
      421 cggagtccgg acctgatgga gtccgatccc agggtgggtc caacggagcg gttctcaagg
      481 atctccaccg ccaccgggag agcgccggcc ctgagcatta tggacagccg ctgcgcctcg
      541 gccgtcgaga agcgccccga gatctgggca ttgccgccac tgatccgttc ctggaccacc
      601 ggtgccgaga tgaccttccc gtcgaggcag atggctatct gtctgcccac attggccgcc
      661 gtcgcctcgt cgaaaagtct tgctccctgg gtgctgaact tgagcgtgac caccggtcgc
      721 ccaaggttgt cgaagtcggt cttggcgtcc tcgaggtcat tgccgctcac ataggcccgg
      781 ccgaggaggt aggcttcacc ctcatcgctc ctggacatta tcatatccgg gtcctcgtcc
      841 gccctgagtc gcatcgcctc ggcggccctg tcgaactgct cgcggaccgc ttcccatctt
      901 gcctgggcct ccaggaactc ctcctgggtg tcgtagtttg gcctctgcgg gcccggaggg
      961 accgttcccg tagattcatg gacgtgcctg aactcaagca acgccgtctt cccgatcagt
     1021 tcaagggccg cctctggatc ggctactccg ggaaggtcca ctattaccct gtcacttccc
     1081 tctctctgta taaccggttc ggtgaccccg tactggtcta tcctgttccg cagaaccgcc
     1141 agaagccgct cgatgctgtc atcggtcagc gggttctcgg aagtgccctg ggcctggagc
     1201 agaatgtggg cccctccctt gaggtccagc cccaggcgga tcctccctcc aatgggaaag
     1261 acggagagca gggcggccac taccactacc gcgacaagga caagccgcca acgatccttt
     1321 ttgagcattt gccttcctcc cctatctgaa accttctatc agtcgggtaa aggcgagtga
     1381 atgcctctac cctcagctga gcatttcata aaaaacggag cggatgcccg gcatccactc
     1441 cgaaggaagc cctgactgga cctctatttt ccaagaaaag ccctggccgg aagtggacta
     1501 gccggcaagg tcttcggata ccgaaggttc aacggcctct ccctcgtgct tcttcatctg
     1561 gatggaggcc ttgaggatcc ttgccttgac cccgtcggcg atctcaacaa tgaagctgtc
     1621 ctccttgacc tctctcacaa cgccgaagaa accgccggcg gtaacgatct ggtcgcccct
     1681 gcgtatcgag gccagcatct cctcgtgttt cctctggcgc ttcttctggg gcctgatgat
     1741 cagaaaatag aagataacga cgaaaactcc caacggcagg agcataccca taagtccacc
     1801 ctgtgcctgc aacggccacg cctccctctc tatgcttgtt ctttacgcaa tattttcgca
     1861 ctgaacattc tatcacgtcg tagttacaat cggaacactt gccccggcgg atctctccct
     1921 caggctacgg aacccttcag gaagaagagg agcttttcga gctcaacgga tatatccacc
     1981 cggtagacca gcacatcctc gggtactgat attgcagtgg tcgcaaaggt caggaccccc
     2041 tttaccgtcc ctgccgccac cgccctgtcg gcggagatct gggcggcccc tgccggcacg
     2101 gtcaggatca ggacctctat ctccttccgg cggatgatct ccgtcatctc ctctgagtca
     2161 tagcagggaa tgccacagat gacctgtccc accttcgaag ggtcgttgtc gaacagggcc
     2221 tccagtttga acttgtcgct cctgagggcc ttgtgaccca acaaagcctc acccagcctc
     2281 cccacgccca caagggcgat cttccagacc cggttgggcg agaggatgtt gctgatgtgc
     2341 tggtacagac ggtccacatg atagccgact ccccgtttgc cgatctcccc gaagtaggac
     2401 aagtccttcc tcacctggct tgccttcagg cccagcatct ctccgatctc ctgggaggag
     2461 accaccctgc gctcctcgtt gtgtagctgc gaaagaagtc tgtaataaag cacaaggcgt
     2521 tcgactgtcg gttcagctat cttcattatt cctgcctcct tgaaaatggc ctgtcccctg
     2581 aaggcacagc tgttcactga tctgcctcca gacattcttg aagatcactc atgaacgcta
     2641 ttttcaccac ggacgctgat cgcccgggac gttcctctca tcggtgacct cgaagcactc
     2701 tcctccccgc ttgaggaggg actcatcaac cgatatcacc ctgagggtga ttatcttcct
     2761 gggaaaagcg acctcgacca catcgccgga cctgagatcc ttcgaagggc ggacctcggt
     2821 gccacccacc cttaccgccc cgtgggttat catctcctgg gcaacgcttc ttcttttgac
     2881 gatccgggac aacttgagaa agcggtctat tcgcaagctt gtgcctccag gaacaatccc
     2941 ccgtgattat tataacaaat gatttgtatc attgcttcaa gaggaacaga tccgatcagg
     3001 gattttcaat cgagaaaatc ccttagcttc ttgctcctgc tagggtgcct tagcttccgg
     3061 agggctttcg cctcgatctg ccttatcctc tcgcgggtga ccccgaagcg tttccccacc
     3121 tcctcgaggg tatagggatg cccatcctcc aggccgaacc tcagccggag aacctccttc
     3181 tccctctcgg taagctcctc gagcatcccc tcgagctgtt cccgcaggag ctggacggcc
     3241 gcagcatcct ctgggctcgg aaggttcttg tcctccagga agtcgctcag ctggctgtcc
     3301 tcctcctcac cgatgggagt ctccagcgag accggttcct gggcgatcct ctgtatctcc
     3361 tcgacccttt cggagccgat ggccatctcg gcagcgatct cctcggcggt cggttcccgg
     3421 ccgagcctct ggactagctg cctggagacc ctgataagct tgtttatggt ctccaccatg
     3481 tggacgggga tgcgtatcgt cctggcctgg tccgcaatgg ccctggttat ggcctggcgg
     3541 atccaccagg tggcgtaggt gctgaacttg aaacccttgc ggtagtcgaa cttctcaacg
     3601 gccctgataa gtcccaggtt gccctcctgg ataaggtcca gaaaaagcat tcccctgcca
     3661 atgtacttct tggctatgct taccacgagc ctcaggttgg cctccacaag cttgctcttt
     3721 ccgacgggat cgccggcttc aacgagcttc gccagctcaa cctcctcctc gggtttaagc
     3781 agggcgatct ttcctatctc cctcaggtac atccttaccg ggtccgaaag gggcaggtcc
     3841 tccaggacgc ccatctcctc attgtactcc gctgcggggg ccgaatcctc gtcacggaga
     3901 tctcccctgg tcttctccat ctccaccagg tctatcccga gctcgttgag gttgaaatag
     3961 agactgtcca gggtctcttc gttcatcatc tccccgggga tatgtttctc gatgtcgtcg
     4021 taggtcacga aacccttctc gcgccccatg tggaggagct ccttgaagct ctcaatgtac
     4081 ctcgccacct ccctgggact ggctacggtc tcatcgccag tatcgtggct ctccatctct
     4141 tcagggctct tcccagcttc ggtcatctct taccggtccc tccttttagc tttcttgcaa
     4201 gttccaggta ttctttcatc tcttcagggg tcgcctggcc cctggcatgc ttctccttga
     4261 gagaggcaaa cttgtccctg gatctcttcg actcgagggc ggaaaccacg atctcccaga
     4321 tctcgccgct ctcgccgttc ctttcgcagt gtgcccagcc agtggagata agtctcatgg
     4381 ggtaggggtc atcggcctcg gcccatctcg acctaagctc ctcgatgctg tctcctgaga
     4441 gtattgacag agccacggtc ttggccccgt cctccgacag gagcgacaac acgtaaggcg
     4501 gatccaccgc tgaccttttc cgctcgtcgg tccagaggag gtagcagagt gccgcctccc
     4561 atgggtccgg aggcctgtcc tcccgggtcc tgtcgttctc cctacttaca tatacgttct
     4621 caggggctga aatgcctttt tccccaactt tggcggccga tcttcgtgcg cttctcagga
     4681 gatcgatcat ctccggcagc agtatgccca gcccctgggc cacctgggag atatgcctgt
     4741 tagcctccag cggagggagt ccggcaagtc cttcgatgat ctccctcctt gcggcggtcc
     4801 ttcgtgcctc gtctgccaga tctgctctct tgaccctgat atggaagagg gggagaggtt
     4861 cggatctcct tgtaagaccc gcaaagcgtt ccgggccctc agcgccgctg acgatatcgt
     4921 cgggatcctt ccccgaagga agcaccacga ccctgatatc aagtccgctc ttctggagga
     4981 cgtacatccc ccttaacgtg gcctcctgac cggccgtgtc ggcatcgtag cagatcaggc
     5041 actggtcgcc gaacctcttc agaagattcg cctgctcccc ggtgagggag gtccccaggg
     5101 aggctaccgt ctcagtgaat cctgccatat gaagcttgat ggcatccatg tagccctcga
     5161 caaggatcga gcggcccctc tcacggatgg acgtcctggc ggagttcagg agatagagga
     5221 tcctcctctt gctgtagagt tcgctctctg ggctgtttat atactttgcg ccctcgccgt
     5281 ccaccaggcg tccgccgaag gcgacaagtc taccctggac atccctgatg gggaacatga
     5341 tcctcccccg gaagcgatcg taggtccttc cgctgctctc ggcaaggagc cccgcggcca
     5401 ttgcttcccc ggtggggatc cccctggtct cgagatcctt tctgagcagg tcccaggaag
     5461 ggggcgccca gccgatctcg aaggctgccg aatcggatga gccgatgccc cttctctcga
     5521 ggtacttcct tgcgctctcg cctcctggcc cggtcaggga tctcctgaaa aaggccaggg
     5581 cagcctccat ggcccccgtt accccgctcg tgctctccct ccgccctccg tagggttcaa
     5641 gtcgaacacc cgccctatcg gccagcatgg tcagagcctc agggaaggtc attccctcct
     5701 tctccattac gaaggagaaa atatccccgc ccctgccgca gccgaagcag tgataggtct
     5761 ggcgttcgga agagacatgg aaggaagggg tcttctcgga atggaaggga cagagtccct
     5821 tgaagctttt accagccctt ctcagctgca ccgtctcgcc tatgaactcg acgatgtcga
     5881 tacggtcctt gatgttcctg acggcatcac ggtccaaaac ggcaccacct tcctgttcgt
     5941 ctctaaggac aggacggggg agggcctcag ggtgccctcc cccgccgata tcgagtccgt
     6001 tggatccttt gtctgttcct gcccgggcga aggggctgcc cttaaatgaa cagcggggcc
     6061 agcaccagcg ctacaacgga catgagcttg ataaggatgt tgaggctggg gccggcggta
     6121 tccttgaagg ggtccccgac ggtgtcgccc acgactgctg ccccgtgggg ctcggagccc
     6181 tttcctccgt agttcccctc ttctatgaac ttcttggcgt tgtcccaggc gcctccggcg
     6241 ttggacatga agatggccat catcacgccc gtaacgatgg atccgccaag gaggccgccc
     6301 agggcctcgg ccccgaggac caggcccaca acgacagggc tgatcacggc caggaagccg
     6361 ggtactacca tctccttaag ggaggcaccg gtggagatat cgacgcatcg ctcgtactcg
     6421 ggttcggcgg tcccttccat tatgcccggt atctcacgga actgcctccg cacttcgtcg
     6481 atcatctgtg aggcggcccg gcccacggcc tggatagtca tggcgctgaa cacgaagggg
     6541 agcatgcctc ctatgaaaag gcccaccatg accttcgggt tgttgatgtc tattaccgtc
     6601 agaccgactg ccgtggcgta ggccgcaaag agggccagag ctgtaag
//
LOCUS       DAOPKQ010000061         6583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_12439_length_6583_cov_4.590380, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000061 DAOPKQ010000000
VERSION     DAOPKQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12439_length_6583_cov_4.590380"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PK146_03315"
     CDS             complement(<1..1280)
                     /locus_tag="PK146_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPE91303.1"
                     /translation="MTAYSAVTREVVRDLERLLGAHNVTVNPEKLASYSRDEVGLQFW
                     DREYRAEVLVFPESTGHVSALLAYADSRRIPVTPRGAGTGLSGGAVPAFGGIVMAFDR
                     MNRILEIDLENLTITAEPGVVTAEIQAAAARHGLEYVGDPCSGDASFIGGNVAENAGG
                     NKVIKYGTTGANVLALEVVLPSGKVTWFGGKRRKDVTGLDLVHLIVGSEGILGVVTRV
                     VLKLVPRQEKVVDLLVPFRTVEEAIALVPRVMREAKLIPSSLEFMDRNSLSVVSRYLG
                     SRLPYQDAGAHLIIQLEGNDEEVLSDQYERVGDLCFENGALEVFVADNRNFREQIWKT
                     RKNIAEAIIVLYTKYAKEDLVVPTSKIPPLLERVSEACRRYSLEAVNYGHVGDGNIHV
                     NLLVDESCEDWHEAIEGARRDLYLATRELGGTLSG"
     gene            complement(1277..2293)
                     /locus_tag="PK146_03320"
     CDS             complement(1277..2293)
                     /locus_tag="PK146_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPE91304.1"
                     /translation="MRRDSHPGGILVLGEVRSGRLHQATLELTAKARILADDLGTEVS
                     TLLLFSGVDVNPETLIGYGADSVICVRDRRLSLFDQEVQSRILYRVIKDIGPDIVLAP
                     ATTSGRSIMPAVAAMLRTGLTADCTGLDIDPETKQLLQTRPAIGGNIMATIRTPEHRP
                     QMATVRPRTFAIPASDPDREGYVSELTLPDHCFESRVVPLGLERSGGDDVNIQDRDVI
                     VSGGKGVKKKENFALLRELAELLDGGVGASRAAVDNKWVPYSHQVGLSGKVVAPKVYF
                     AVGISGTVQHLAGMQTAGLIVAINKDPEAPIFRVADFGLCGDLFDIVPRLIDALRGRK
                     GVCT"
     gene            complement(2290..3096)
                     /locus_tag="PK146_03325"
     CDS             complement(2290..3096)
                     /locus_tag="PK146_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPE91305.1"
                     /translation="MKIAVLIKQVPDSDDVRMDEATGTMVREGVGTMINPLDLHALQE
                     ALKIRSSWGGKVTAISMGPPQAESALREALALGADGAVFLSDPLFAASDTWSTARTLA
                     AAIEKTGPYDLILAGEKATDGETGQVGPEVAVMLDVPFSTYVSGIEFESGNSLVVSRT
                     VEDGIERQRLSIPCLLTVLRDINEPSMPTLSGKRAARRADLPVWDLDRLGLSPEEIGL
                     KGSPTRVVRIFHPQITRSPRLFNGDDIERGIAELLIKLEDLGITGREGVS"
     gene            complement(3256..3332)
                     /locus_tag="PK146_03330"
     tRNA            complement(3256..3332)
                     /locus_tag="PK146_03330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3296..3298),aa:Arg,seq:ccg)
     gene            complement(3892..6243)
                     /locus_tag="PK146_03335"
     CDS             complement(3892..6243)
                     /locus_tag="PK146_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPE91306.1"
                     /translation="MRVEERTAELVEIPSVLERMSRRTRSETGKLLLQGMLPAISVKG
                     ARERQDLLRFFMRYRETRGEMTWDGRLKPLAPLFDSARETALLTGAELVQFRILISLS
                     KRIREDIRDARGEIPGIERLAEGIRDLEKELDRLQVISDDGQLYDSASEELTRVRNSL
                     RDITSRARTKCSELSSRANLAPMLQDRVVHFRKGRFALLVRVEDSGRFDGIAMDRSSS
                     GKGVYIEPKEVVPLNNRIEILRGEEREEERRILSSLTAMLLEKESHIAETELALARID
                     VLYAGAQFLDERSWILPELDGRSRFHFRDLWNPLIGRGCVPVSVHCGERFRQLVITGP
                     NTGGKTVVLKSVAIAIFLALSGLPVPAAEGTLVGGIDYLAADIGDEQGIEQSLSTFSS
                     HVRRIITMMEKAGKDSLLMLDELGAGTDPQEGAALGIAILEALKGRRSLVLATTHHNP
                     IKRYALGSRGVETAGMEFDPVTLAPTFRLIMGIPGKSNALFIAEKLGMPRDVIESARE
                     HLSGEARTTEQIMSELFDKQAELESAGMTMAEERKELASLRRELEEKIRRINDRQEKV
                     IEAADRRAREVMEEAERSAREMLSRLEGAAESAARREYGEVKKETQPLKERISRRENA
                     LAKKRVERDGIPQVGDTVRLGGTEARGVVEAVEGGKAVVVAGSMRVEAPLESLVLVKE
                     KASQAEEKKAPIIHAPAPRGVPSSIMVRGMTVDEALPEVEVYLDRAFRAGYGEVVIIH
                     GRGEGILRRAVHSLCRSLPYVTYFRLGSAGEGGYGVTIVSFRR"
     gene            complement(6240..>6583)
                     /locus_tag="PK146_03340"
     CDS             complement(6240..>6583)
                     /locus_tag="PK146_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPE91307.1"
                     /translation="ILILVAGVYAGRAVKAFLKFTHLSGIDRGMGIVAGAVKAFALLI
                     IVYAALMAFSPVLSPYWMNESVAMKLADRSWPSVQNALSSIRLLDPGSLPWHLTPPVE
                     GERPKDPEPSN"
BASE COUNT         1244 a         2111 c         1723 g         1505 t
ORIGIN      
        1 cccgaaaggg tcccccccag ctctcttgtt gccaggtaca ggtctctcct tgccccctcg
       61 atggcttcat gccaatcttc gcagctctca tcgacaagga ggttcacatg gatgttaccg
      121 tcgccgacgt gcccgtaatt gaccgcctca aggctgtacc tcctgcaggc ctcgcttacc
      181 ctttccagga gcggtgggat cttgctggtg ggtacaacca ggtcctcctt ggcgtacttc
      241 gtgtaaagga caattatcgc ctcggcgata ttcttgcggg tcttccagat ctgttcacgg
      301 aaattccggt tgtccgcaac gaagacctcc agggcgccgt tctcgaaaca gagatccccg
      361 accctctcgt actggtccga gaggacctcc tcgtcgttcc cctcaagctg gatgataagg
      421 tgtgccccgg catcctgata ggggagcctg cttcccaggt acctggagac aacgctgagg
      481 gaattccggt ccatgaactc cagtgaggag gggatcagct tggcctccct catgaccctt
      541 ggcaccaggg ctatcgcctc ctctactgtc ctgaagggca caaggagatc gactaccttc
      601 tcctgcctcg ggacaagctt gagcaccacc ctggtcacga cacccaggat accctcggag
      661 ccgacgataa ggtggaccag gtccagtcct gtaacatcct ttcgtctctt tcccccgaac
      721 caggtcacct ttcctgaggg gagtaccacc tcgagggcca gaacgtttgc ccccgtcgtt
      781 ccgtatttga tgactttgtt gccacccgcg ttctccgcca cgttgcctcc aatgaaggag
      841 gcatctccgc tgcagggatc cccgacgtac tcaagaccgt gccgggccgc cgcagcctgg
      901 atctcggcgg taacgacccc cggctctgcg gtgatagtaa ggttttccag gtctatttcc
      961 aaaatgcggt tcatcctgtc gaaggccatc acgatcccgc cgaaggccgg aacggcacca
     1021 ccggagaggc ctgtcccggc cccccggggc gtaacaggta tccttcggga gtccgcatag
     1081 gcgaggagcg ccgatacatg tccggtgctc tcgggaaaga caaggacctc ggcccggtac
     1141 tccctgtccc agaactgaag cccgacttca tcacgggagt aggacgcaag cttctccgga
     1201 ttcactgtca cgttatgagc ccccaggagt ctttcgaggt ccctgacgac ctcccgggtt
     1261 accgctgaat aggctgtcat gtacagaccc cctttctgcc cctgagagca tctatcagcc
     1321 gggggactat atcgaaaaga tccccgcaga gtccgaaatc cgcgacgcgg aatatcggag
     1381 cctcaggatc cttgttgata gctactataa gccccgccgt ctgcataccc gcaaggtgct
     1441 gcactgtacc cgatattcct acggcaaaat atactttcgg ggctactacc ttgccgctga
     1501 gaccaacctg gtgagagtag gggacccact tgttgtccac tgccgcccgg ctggccccca
     1561 cccctccgtc gaggagttcc gccagttccc ggaggagagc gaaattctcc ttttttttca
     1621 ctcccttacc ccccgatacg atcacgtcgc ggtcctggat gttgacatcg tcacccccgg
     1681 agcgttcgag gccgaggggg accacccggc tctcgaaaca gtgatcgggg agggtcaact
     1741 cgctgacgta accttccctg tcggggtccg atgccgggat cgcgaaggtc ctcggtctga
     1801 ccgtcgccat ctgcgggcgg tgttcagggg tccttatggt ggccatgata ttcccaccga
     1861 tggccgggcg ggtctgaagg agctgcttcg tctccggatc tatatcgagt ccagtgcagt
     1921 cggcggtgag accggtcctc aacatggcag caacggctgg catgatactc ctccccgatg
     1981 tagtggccgg agcaaggact atgtctggtc caatatcctt aatgaccctg tagaggatcc
     2041 gggactggac ctcctggtcg aagagactta ggcgcctgtc ccttacgcag atcacactgt
     2101 cagccccgta gccgatcagc gtttcggggt tcacatccac cccggagaac aacaacaggg
     2161 ttgacacctc tgtgcccagg tcgtcggcca gaattcgagc cttggctgta agctccagtg
     2221 tggcctgatg aagccgcccc gaccttacct ccccgaggac aagaatgcca ccagggtgag
     2281 agtctcttct catgaaactc cctcccttcc agtgatcccc aggtcctcga gctttatcaa
     2341 gagttcggct atgcctctct caatgtcatc gccattgaaa agcctgggtg atcttgtgat
     2401 ctgggggtga aagatcctta ccaccctggt gggggacccc ttgaggccta tctcttccgg
     2461 ggaaagaccg agacgatcca ggtcccagac cggaagatcg gcccttcgtg cggctctctt
     2521 tccggaaagg gtgggcatcg acggttcgtt tatgtccctc aggaccgtaa gcagacaggg
     2581 aatggagagc ctctgcctct ctattccgtc ctcgactgtt cgtgagacca ccagggagtt
     2641 gccactctca aattcgatcc cgctgacata ggtgctgaaa ggcacgtcca gcatcacggc
     2701 cacttccggt ccgacctgac ccgtttcccc atcggtcgcc ttttcaccgg caagaatgag
     2761 gtcatagggt ccggtttttt ctatggccgc agcaagggtt ctggccgtag accatgtatc
     2821 tgatgccgca aagaggggat cgctgaggaa gaccgccccg tcggccccga gggccaaagc
     2881 ctctctgagg gccgactccg cctggggcgg ccccatcgat atggcggtga ccttcccacc
     2941 ccatgaagac ctgatcttga gtgcttcctg gagtgcatga aggtccaggg ggttgatcat
     3001 agtccctacg ccctcccgga ccatcgtccc tgttgcctcg tccatacgga cgtcatcgga
     3061 atcggggacc tgttttatca gaacggcaat cttcactgaa ccacatcctc gaaagggaag
     3121 atcagggccc ttgatataac agaaaaccgg ctcttccctc tggaaggacc ggcagcagta
     3181 tcagagcccg cagaagatct gatgatcgat ggtaaaaaca acgaattaac aggagaactg
     3241 gacccggaaa gaatatggcg tgcccgtcgg gatttgaacc cgagaccttc ggctccggag
     3301 gccgacgctc tatccagctg agctacggac acgcatccgc ggcgcttagg gtaccgcccg
     3361 gaacatatgg ttgtcaagta tatctgtgac tcgtgactcg gaacccgtaa ctcgtaccct
     3421 gtcgctgcgc tcggggcacc ttgtaattcg tgaatcgtga atcgaaaaac caaaagtcaa
     3481 aggcgtaccc tgtaaactga ggtctgcagc ttgcctcctg gtttttctgc ccatcccatt
     3541 ttccgttgac gatcttatcg acatctatgt cttccgcctc acggatcttg gcctttcctg
     3601 cagccagtct cctcggtttt ctgcccttcc gtcagacctc tcacctctcg tctcgtctca
     3661 cgttcttggt cttacggtat ttccatatat tcgctcttct ctgcggccct ctgtgctctc
     3721 tgcggtgtat tagggtattt ccatatattc gctcttctct gcggccctct gtgctctctg
     3781 cggtgtatta gggtatttcc atatattcgc tcttctctgc ggccctctgt gctctctgcg
     3841 gtgcaaaggc tttgcctttg acttggtttt cccgagtcac gagttacgca actaccttct
     3901 gaacgaaact atagtcaccc cgtaaccgcc ctcaccagca ctgccgagcc ggaaatatgt
     3961 gacatagggc aggctcctgc acagggagtg gaccgccctt ctcaggatcc cctccccgcg
     4021 accgtggatt ataaccactt ccccgtagcc ggcccgaaag gccctgtcaa ggtagacctc
     4081 cacctccggc agtgcttcgt ccacggtcat acccctgacc atgatcgagg atggtacgcc
     4141 cctcggcgcg ggagcatgta tgatcggggc ctttttttct tcggcctggg acgccttctc
     4201 cttcaccagt acaaggcttt caagaggggc ttctaccctc attgaaccgg cgaccaccac
     4261 cgccttgccg ccctcgacgg cttcgaccac tcccctggcc tccgtcccgc caagccttac
     4321 ggtgtctccc acctggggga tgccatctct ctcgaccctc ttttttgcaa gggcattctc
     4381 ccttcgggat atcctctcct tcaaaggctg ggtctccttt ttcacctctc cgtactccct
     4441 ccgggcagcg gactcggccg ctccctccag tcgggaaagc atctccctgg ccgatctttc
     4501 ggcttcctcc attacctctc tggctctcct gtcggcagct tcgatgacct tttcctgcct
     4561 gtcgtttatc ctccttatct tctcctcgag ctccctccta agggaggcaa gttccttcct
     4621 ctcctctgcc attgtcatcc ccgcgctttc aagttcggcc tgcttgtcga agagttccga
     4681 cattatctgt tccgtcgttc tggcctctcc cgaaagatgt tccctggcag actctatgac
     4741 gtctctcggc attcccagct tttctgctat aaaaagggcg ttgctcttcc ccgggatccc
     4801 catgatcagc ctgaacgtcg gggcgagggt caccggatcg aactccatcc cggccgtctc
     4861 tactcccctg ctccctaagg cgtagcgctt tatcgggttg tgatgggtag tggcgaggac
     4921 cagcgacctc cggcccttaa gcgcctccag gatagctatt ccaagcgcag caccctcctg
     4981 ggggtccgtt cccgccccga gctcgtcgag catcagaagg ctgtccttcc ctgccttctc
     5041 catcatggtt atgatccttc tgacgtggga gctgaaggtt gaaaggctct gctctattcc
     5101 ctgttcatca cctatatcgg ccgccaggta gtcgatcccg cccaccagag tcccttccgc
     5161 cgccgggacc ggaagacccg aaagggccag gaatatagca atggccaccg atttcagcac
     5221 caccgtcttt ccgcccgtgt tcggtccggt aatgacaagc tgccggaatc tctctccgca
     5281 gtggacgctc acaggaacgc accctctgcc gatcaggggg ttccacaggt cccggaagtg
     5341 gaaacgggac cggccgtcca gctcgggcag gatccaggag cgttcatcca ggaactgggc
     5401 gcctgcgtag aggacatcga tcctcgcaag ggccaactct gtttcggcga tgtgggactc
     5461 cttctccagg agcatggctg tcagcgagga caggatcctt ctctcctctt ccctctcctc
     5521 gcctcgcagg atctctatcc tgttgttcag gggaacgacc tctttcggct caatatacac
     5581 gcctttgccc gaagaggacc tgtccatggc gatcccgtcg aatcgcccgg aatcctcgac
     5641 cctgacgaga agggcgaacc gccccttcct gaagtgcacg accctgtcct gaagcatggg
     5701 agccagatta gccctggagg agagctctga acacttggtc ctggcccggg aggtgatatc
     5761 cctgagggaa ttcctgaccc tggtcagctc ttccgatgcg ctgtcataaa gctgcccgtc
     5821 atcggatatc acctgaagcc tgtccagctc cttctccaga tcccggatgc cttcggcgag
     5881 cctttcgata ccggggatct ccccgcgggc atctctgata tcttccctta tcctcttgga
     5941 aagactgatg aggatgcgga actggaccag ttcagccccg gtgagaagag ctgtctccct
     6001 ggcgctgtca aagagaggtg ccaggggctt cagtcttccg tcccaggtca tttcgccacg
     6061 ggtctccctg tagcgcatga agaacctcaa aagatcctgc cgctcccggg cgccctttac
     6121 agatatggcc gggagcatcc cctggagcaa aagcttgccg gtctccgatc ttgtccttct
     6181 tgacatcctt tcaagaacgg agggaatctc gaccagttcg gccgttcttt cttcaaccct
     6241 caatttgaag gctccgggtc tttcggcctc tccccttcta cgggaggggt caggtgccac
     6301 gggagcgacc cgggatcaag caaccttatg gaagagaggg cgttctggac cgatggccac
     6361 gacctgtcgg cgagtttcat tgcaacactt tcgttcatcc agtagggcga caagacagga
     6421 gagaaggcca tcagggcagc gtagactatg ataaggaggg caaatgcctt cacggcacct
     6481 gcaactatac ccatgcccct gtcgatgccg gacaggtgag tgaacttgag gaaggccttc
     6541 acggcccttc ctgcgtagac ccctgccacg aggataagga tat
//
LOCUS       DAOPKQ010000062         6583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_12441_length_6583_cov_4.149969, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000062 DAOPKQ010000000
VERSION     DAOPKQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12441_length_6583_cov_4.149969"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /gene="groL"
                     /locus_tag="PK146_03345"
     CDS             <1..1524
                     /gene="groL"
                     /locus_tag="PK146_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162755.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPE91308.1"
                     /translation="NVVLEKKFGSPTITNDGVTIAKEIELEDPFENMGAQLLKEVASK
                     TNDIAGDGTTTATVLARAVILEGMKNVAAGANGMLMRHGIEKAVDLVVDELKSMSINV
                     KEREKIAQVAAISANDHAVGELIAEAMEKVGEEGVITVEDSQTVGTTLEMVEGLQFDK
                     GYVSPYMMTDPERMEAHLDDAYILINDGKISSVKDMVPLLEKVVQTGKPLLIIAEDVE
                     GEALATLVVNKLRGTMQVVAVKAPGFGDRRKAMLQDIAIVTGGQVISEEIGIKLENAD
                     LAMLGKAKKVRVNKEETTIVEGAGDPDSIRKRAAQIKAELEESTSEYDKEKLQERLAK
                     LVGGVAVIQVGAATETEQKELKHRIEDALNATRAAVEEGIVAGGGVALVNAIEPLDKF
                     IEKLNGDEKTGATIVKKALTSPIHLIADNAGYQGDVVVEKVRGLKKGHGFNAVTGEYE
                     DLIKSGIIDPVKVTRSALQNAGSIASMVLTTETLIADKPKKESDMPPMPGGMGGMDY"
     gene            1653..3203
                     /gene="guaA"
                     /locus_tag="PK146_03350"
     CDS             1653..3203
                     /gene="guaA"
                     /locus_tag="PK146_03350"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPE91309.1"
                     /translation="MSSTEREIIIIIDCGSQYTQLIARRVRELGVYSEIIPWDSSLEA
                     VMAKKPSGLVISGGPGTVLEDDSPWVPRELLDGRFPVLGICYGMQLIARILGGEVRKG
                     ELAEYGRARVFLEDERSGVLKGLPGDLEVWMSHWDYVEIPPPGFVVTARSESGMIAGF
                     QGPDSKISAIQFHPEVAHTSGGMDILANFLFGICRCGKTWSLEDWIGSTVKEIQATAG
                     EGRVVCGLSGGVDSTVAAVLTSKAIGDRLECIFVNNGLLRLKEAEQVMETYRGSLGLK
                     VHYVDATDDFLSALEGVTEPEAKRKAIGETFIRVFERKARGIGGADWLLQGTLYPDVI
                     ESGHKGKDAAVIKSHHNVGGLPEKMDFQVLEPLRDLFKDEVRKIGAMLGVPKEILGRH
                     PFPGPGLAVRCLGDVTRERLDTLRKADAIFVSEIRDQGLYDRMWQAFCVLLPVRTVGV
                     MGDIRTYAEVIALRAVESPDGMTADWVRLPGELLDRVSRRICNELGGANRVVYDITGK
                     PPATIEWE"
     gene            3260..4369
                     /locus_tag="PK146_03355"
     CDS             3260..4369
                     /locus_tag="PK146_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPE91310.1"
                     /translation="MDNEVTSRPQGQWGVYLPFIVAFAMLSMLSYIVSFPLFRPLAWS
                     SLLSFFAYPLYHLIYMRAFSGKWSNLAAGITTAAILFLLVVPAGLMGTLIAREGIKVY
                     GKVADLLLQFEGAEMPSLLEILPKGLSARLAPYMEHFEFIGDAFRQAGRWFASNIASA
                     SRGFLGNAFRMFYQLIVIAVASFFMLRDGHKILGYIKEILPLSSEERDDIFGKAKQML
                     QSVVYGITFTAGIQAFLGGFGWWWVGLPSPALFAGAMFLLAMIPFVGTPMVLLPGAAY
                     LFAVGKIGPAIFLAIWALLVVSTIDNFIRPLFISEGSKVHILIVFTGVVGGLAAWGFI
                     GLFTGPLILSMFFFFLDSYRRIWQRMRGDNQENEK"
     gene            4386..5024
                     /locus_tag="PK146_03360"
     CDS             4386..5024
                     /locus_tag="PK146_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HPE91311.1"
                     /translation="MPFIIAVSGFKNSGKTTLCRKLFPLLKDAGLDVAYVKRTHEEVL
                     SPEGTDSGQLAEVSGAVALWGPDGLRIEDSRKDLDPGSLAARFFPGRHLLLLEGGKNI
                     PLPKIWVGSPEAIPEEVSGVVAFYDRDNPCEKERHFCAGQEKELARLVLAMVRKAEAS
                     PAEVYSREKRIPVKAFVGEFIAGALSGMLRSLKGDFDPEEGISVHIRSLPGE"
     gene            5195..>6583
                     /locus_tag="PK146_03365"
     CDS             5195..>6583
                     /locus_tag="PK146_03365"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPE91312.1"
                     /translation="MLAQIVWISGGAGLLALAFALFSSGRINSFEVTNDRVNELSGII
                     QRGSMAFLSREYRALIPFVVVVGGILWFKISSATAISFLLGALCSGLAGFVGMRVATR
                     ANGKTAFAAGQGMNPALRIAFTGGTVMGMSVVGIGIIGIIVCYWLFKDPNVITGFGFG
                     ASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDIAGM
                     GADLFESYVNSIIAAMAIGLVAFGDTGVVYPLLLASLGILSAVLGTFFVRVREGGDPQ
                     LALRIGTLATGAFMIGGSFFMTRAIFGDITLFWAVMSGVAVGVLIGLVTEYYTSSSYS
                     PVQSIASASETGCATNILAGLGVGMKSTAVPVLLICGAILVGVRFGGLYGIACAAVGM
                     LSITGMSLSVDAYGPIADNAGGIAEMSHMPPEVRKITDKLDAVGNTTAAIGKGLAIGS
                     AALTALALFAAYA"
BASE COUNT         1364 a         1849 c         2031 g         1339 t
ORIGIN      
        1 aacgtggtgc tggagaagaa gttcggttcg ccgacgatca ccaacgacgg cgtgaccatc
       61 gcgaaggaaa tcgagctgga agatcccttc gagaacatgg gagcccagct ccttaaggaa
      121 gtcgcatcca agaccaacga catcgccggt gacggaacga ccaccgccac tgtccttgca
      181 agggccgtca tccttgaagg gatgaagaac gttgcggcag gtgcaaatgg aatgttaatg
      241 cgtcacggca tagagaaggc tgtcgacctt gtcgtagacg agctcaagtc aatgtccatc
      301 aacgtcaagg agcgggagaa gatcgcgcag gttgccgcca tctctgccaa cgaccacgct
      361 gtgggtgaac tgatcgccga ggccatggag aaggtcgggg aagagggcgt cataaccgtc
      421 gaggacagcc agactgtcgg aactaccctg gagatggtag agggtctcca gttcgacaag
      481 ggctacgtga gtccctacat gatgaccgac cccgagcgga tggaggccca cctcgatgat
      541 gcctacatcc tcatcaacga cggcaagata agcagcgtga aggatatggt ccccctcctc
      601 gagaaggtgg tccagaccgg aaagcctctt ctgattatcg ccgaggatgt cgagggtgaa
      661 gcccttgcga ccctggtggt gaacaagctc cgcggaacca tgcaggtcgt tgccgtaaag
      721 gcccccggtt tcggcgacag gcgcaaggcc atgctccagg atatcgctat cgtgacaggt
      781 ggtcaggtca tctccgagga gatcggcatc aagcttgaga acgccgacct cgccatgctc
      841 gggaaggcca agaaggtacg agtgaacaag gaagagacca ccatagtcga gggcgccggc
      901 gatcccgatt ccatcaggaa gcgcgccgcc cagatcaagg ccgagcttga ggagtccact
      961 tccgagtacg acaaggagaa actccaggag cgccttgcca agctcgtcgg cggagtggcc
     1021 gtcatccagg tcggagccgc caccgagacg gagcagaagg aactgaagca ccgtatcgag
     1081 gacgccctca acgcgacaag ggctgccgtc gaagagggta tcgttgccgg aggcggcgta
     1141 gcactggtca acgccatcga gccccttgac aagttcatcg agaagctcaa cggtgacgaa
     1201 aagaccggcg caaccatcgt caagaaggcc cttacgtccc cgatccacct tatcgcagac
     1261 aacgccgggt accagggcga tgtcgtggtc gagaaggttc gcggcctcaa gaagggtcat
     1321 ggctttaacg ccgttaccgg cgagtacgag gaccttatca agtccggtat catcgacccc
     1381 gtaaaggtta ccaggagcgc ccttcagaat gccgggtcga tagcttccat ggtgctcacc
     1441 acagagaccc tgatcgcaga caagcccaag aaggaaagcg acatgccccc gatgcccggc
     1501 ggcatgggcg gaatggatta ctaatacggt ccttcaaaat atccggggcc ccctcgcggg
     1561 gccctttttc attgtgagcg ctcggcgcgg attctgataa acttgtgctg tgatcgccgg
     1621 gccgtatcgg gtcgggcacg gaggtgaagc ttttgtcttc cacggagaga gagatcatca
     1681 taatcatcga ttgcggttcc cagtacacac agctgatcgc aaggagagta cgggagctgg
     1741 gggtctacag cgagatcatc ccctgggaca gcagcctcga agccgttatg gcgaagaaac
     1801 cctctggtct cgtcatttcc ggaggaccgg gcactgtcct ggaggatgac tccccctggg
     1861 tcccccggga actgctggac ggaaggttcc ctgtcctcgg gatctgctac gggatgcagc
     1921 tgatagccag gatcctgggc ggagaggtca ggaaggggga gctggcggag tacggaaggg
     1981 ccagggtctt ccttgaggat gaacgaagcg gggtcctgaa aggtcttccg ggggatctgg
     2041 aggtctggat gagccattgg gactacgttg agatcccccc tccgggtttc gtggtgactg
     2101 ccaggagcga gtctggcatg atagcgggct tccagggccc cgactcgaaa atatctgcca
     2161 ttcagttcca ccccgaagtg gcccacacat cgggaggaat ggacatcctt gcaaacttcc
     2221 tcttcgggat ctgccggtgc gggaaaacct ggagcctcga ggactggatc ggctcgaccg
     2281 tcaaggagat ccaggccacc gcaggtgagg ggcgcgtggt ctgcggtctt tccggaggag
     2341 tagattcgac ggtagccgct gttcttacat cgaaagccat aggtgaccgc ctggagtgca
     2401 tcttcgtaaa caacggtctt ctccgcctga aagaggcgga acaggtaatg gagacctacc
     2461 gtggctccct gggcctgaag gtgcactacg ttgatgccac cgacgatttc ctctcggctc
     2521 tggagggggt taccgagcct gaggcaaaaa ggaaggccat cggggagacc ttcatcaggg
     2581 tcttcgagcg gaaggccagg gggatcggag gagccgactg gctcctgcag ggaaccctct
     2641 accccgacgt gatagagagc ggccacaagg gaaaggacgc agccgtcatc aaaagccacc
     2701 ataacgtggg aggacttcct gaaaagatgg acttccaggt acttgaaccc ctgagggatc
     2761 tcttcaagga cgaggtcagg aagatagggg ccatgctggg agtgccgaag gagatcctcg
     2821 ggcgccaccc cttcccgggg ccggggcttg cagtccgctg ccttggcgat gtgacccgcg
     2881 agaggctcga tactctgagg aaggccgatg ccatcttcgt atccgagatc agggatcagg
     2941 ggctctacga caggatgtgg caagccttct gcgtacttct cccggtgagg acagtaggtg
     3001 ttatgggaga tatcaggacc tatgccgagg tcatcgcact gagggccgtg gagtcccccg
     3061 atggcatgac agcagactgg gtgagacttc ccggcgagct gctcgacagg gtatccagga
     3121 gaatatgcaa tgaacttggc ggagcaaaca gggtggtcta cgatatcacc gggaaacccc
     3181 ccgcgaccat tgaatgggag tagaccctct ccaggtctgc aaatgaaagc ggaagaagga
     3241 acggaggtgt caaacggtct tggataacga ggtgacgtca agaccccagg ggcagtgggg
     3301 agtctacctg cccttcatag tggcctttgc aatgctctcc atgctctctt atatcgtctc
     3361 cttccctctc ttcagacccc ttgcctggtc gtcgctgctc tccttcttcg cctaccctct
     3421 ttaccacctg atctacatga gggctttttc ggggaagtgg tccaaccttg ctgcgggaat
     3481 aacgacggcg gcgatacttt ttctcctggt ggttcccgcg ggtctcatgg gcaccctgat
     3541 agccagggag ggtatcaagg tctacggtaa agtggcggac cttcttctgc agttcgaagg
     3601 agcggagatg ccttctctgc tggagatact acccaagggg ctttcggcca ggcttgcccc
     3661 atacatggag cacttcgaat tcatcggcga cgcctttcgt caggctggaa gatggtttgc
     3721 ctcaaacatc gcctcagcct ccaggggttt tctgggcaac gctttcagga tgttctacca
     3781 gctgatagtc attgccgtgg cctctttctt catgctgagg gacggccaca agatcctcgg
     3841 ctacataaag gagatcctcc ccctctcctc cgaggagagg gatgatatct tcgggaaggc
     3901 aaagcagatg ctccagtcgg tggtctacgg aataaccttc accgctggaa tccaggcctt
     3961 tctgggcggg tttggatggt ggtgggtggg actgccaagt ccggcccttt ttgcgggagc
     4021 catgttcctc ctggccatga tccccttcgt tgggacgcca atggtgcttc tgccgggggc
     4081 ggcctacctc tttgcggtcg gaaagatcgg tcctgccatc ttcctcgcga tatgggcgct
     4141 actcgtggtg agcaccattg acaacttcat cagaccactg ttcatatccg agggcagcaa
     4201 ggtacacatt cttattgtct ttaccggggt tgtaggcggg ctggccgcct ggggtttcat
     4261 tggtctcttc accggaccgc tgatcctctc catgttcttc ttcttcctgg acagctacag
     4321 gaggatctgg cagcgcatga ggggagataa ccaggaaaat gaaaagtgaa cggaggtaag
     4381 acgctgtgcc ttttataatt gccgttagcg gtttcaagaa cagcgggaag accaccctgt
     4441 gcaggaagct gttccccctt ctgaaagatg ccgggctcga tgtagcctac gtcaagagga
     4501 cccatgagga ggtcctctcg cctgagggga ccgattcggg ccagctcgcg gaggtctccg
     4561 gggccgtcgc gctatggggt cccgacggat tgcggataga ggacagcaga aaggacctgg
     4621 acccaggctc cctggcggca cggttctttc caggcaggca cctcctcctt ctggaggggg
     4681 gaaagaacat tcccttaccc aagatctggg taggcagccc tgaggctatc ccggaagagg
     4741 tctcgggggt ggtggccttc tacgacaggg acaacccgtg tgaaaaagag agacacttct
     4801 gtgcgggcca ggagaaggaa cttgcccggc tggtgcttgc aatggtccgc aaggccgagg
     4861 cctcccccgc cgaggtatat agccgcgaga agaggatccc cgtcaaggcc ttcgtgggtg
     4921 agttcatagc cggcgctctt tcgggcatgc taaggtctct caagggggat ttcgaccccg
     4981 aagaggggat aagcgtccat ataaggtccc ttccgggaga atagggccag tacggtcgca
     5041 tatccattat ccatcttgcg gttaagtgct agggtgtcta taatgttgtt cgcttgccga
     5101 aaaaggcagc gatcccgctt tatccggcgc ccgcccagga ggcgggctgt cttttgctgt
     5161 tcaagacttc atatgatacg gggaggttct ttgaatgttg gcacagatcg tttggatttc
     5221 cggaggggcc gggcttctcg cacttgcgtt cgcccttttc tcgtcggggc gcatcaattc
     5281 tttcgaggta accaatgaca gggtgaacga actttccggg atcatacaga ggggttccat
     5341 ggcctttctc tccagggagt acagggcgct gatccccttc gtagtggtcg tcggagggat
     5401 cctctggttc aagataagct ccgccactgc catctccttc cttctcgggg ctctctgcag
     5461 cggccttgcg ggctttgtgg gcatgcgggt ggcgaccagg gccaacggaa agacggcctt
     5521 tgccgccggg caggggatga acccggcgct caggatagcc ttcaccggcg gaacagtaat
     5581 gggaatgtcc gtcgtcggca tcggcataat cggcattatt gtctgctact ggctcttcaa
     5641 ggatcccaac gtcataaccg gcttcggttt cggggccagc tccattgccc tcttcgccag
     5701 ggtcggcggc ggtatataca ccaaggccgc cgacgtcggt gccgacctgg ttggcaaggt
     5761 tgaggccgga atacccgagg acgaccccag gaacccggcc gtgatcgccg acaacgtcgg
     5821 cgacaacgtg ggcgatatcg ccggaatggg cgcggatctt ttcgaatcct acgtcaactc
     5881 catcatcgcg gccatggcca tagggcttgt ggccttcggt gacaccgggg tagtctaccc
     5941 cctgctcctc gcttcccttg gcatactctc cgctgtcctg gggaccttct tcgtcagggt
     6001 ccgcgagggc ggtgaccccc agctggccct caggatcggg actctcgcga cgggagcctt
     6061 catgataggc ggctccttct tcatgaccag ggcgatcttc ggggacatca ctctcttctg
     6121 ggcagtcatg tccggagtcg cagtgggtgt ccttatcggt cttgttaccg aatactacac
     6181 ctcctcgtcc tactcgccgg tccagagtat agcctccgca tccgaaacag gctgtgccac
     6241 caatatcctt gcaggtctcg gcgtcggcat gaagtcaacg gcggttcccg ttctgctgat
     6301 ctgcggggcc atcctggtgg gcgtcaggtt cggcggattg tacgggatcg cctgcgcagc
     6361 cgtgggaatg ctctctatca ccgggatgtc cctcagcgtc gacgcctacg gacctatcgc
     6421 cgacaatgcc ggaggcattg ccgagatgag ccatatgcct cccgaggtcc gcaagatcac
     6481 ggacaaactt gacgccgtgg gcaacacaac agctgccatc gggaaaggcc tggccatagg
     6541 ttcagccgcg cttacagctc tggccctctt tgcggcctac gcc
//
LOCUS       DAOPKQ010000063         6483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_12694_length_6483_cov_4.507001, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000063 DAOPKQ010000000
VERSION     DAOPKQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12694_length_6483_cov_4.507001"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK146_03370"
     CDS             <1..485
                     /locus_tag="PK146_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPE91313.1"
                     /translation="ALAGLFGAVGTTSYTENIGLIGLTGVASRWVVRTGAVILIIMSM
                     IGKLGALIATVPSPVIGGAYIALFGIIGALGIQVLMRADMGSQRNVLIVGFAFLMALG
                     LPGWTEGQQEAFFAFGIPGQMAWAILKTPMAVAGICAAFWDSIVPGTPEERGIGTRLK
                     "
     gene            686..1060
                     /locus_tag="PK146_03375"
     CDS             686..1060
                     /locus_tag="PK146_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPE91314.1"
                     /translation="MAKQLDIFKCEVCGNVVELLHVGGGELVCCGQPMTLMEEKTADS
                     ATEKHVPVIEKKDGGILVKVGSIPHPMEDKHFIEWIQLIVDGKNCKKFLKPGEAPEAF
                     FSENGDNVSAREYCNIHGLWRS"
     gene            1066..1560
                     /locus_tag="PK146_03380"
     CDS             1066..1560
                     /locus_tag="PK146_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPE91315.1"
                     /translation="MITKKMQTALNKQINEELFSSYLYLSMAADFEARNLTGAAKWMK
                     MQADEELEHAMKFFHFVLERGGEVDLLAIAKPQKSWKSPLTAFQDAEKHEQHITKCIN
                     DLYEMATKEKDYATQSMLKWFVDEQVEEEASVGEIVARLEMIADSKNGLFMMDRELGK
                     REED"
     gene            1701..2228
                     /locus_tag="PK146_03385"
     CDS             1701..2228
                     /locus_tag="PK146_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPE91316.1"
                     /translation="MEVAIDKKALYALSYGLYVVSSRKGNKLNGQVANAVMQVTADPV
                     SVVACLHRDNMTTECISDSGIFSVSILEEDTPMTFIGNFGFKCGRDYDKFGKCSYELG
                     ETGAPLVIDHSIAVIEARVVKNVDIFTHVMFFGEVVASRHIREGRPLTYAHYHEVKKG
                     KSPKNAPTFTLNEVR"
     gene            2246..3445
                     /locus_tag="PK146_03390"
     CDS             2246..3445
                     /locus_tag="PK146_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPE91317.1"
                     /translation="MNTLEIMKDVHWVGAIDWDLRDFHGYSTEKGSTYNAFLVVDEKT
                     TLMDTVKKKLADELFSRIREVNPSGRIDYLVLNHAEMDHTGSLTEVFRAFRPEKIVCS
                     APCRDAIIEHFGKNDWPFHVVRTGDTLSLGKRTIRFIETKMLHWPDSMFSYLEEDRLL
                     ISSDAFGQHFATGERFDDEVDPLELVKQAAKYYANILLPYSGLAAKLLESIKAMGLKI
                     DMIAPDHGLIWRKGVADILKAYGDWSSQKALPKAVVAYSTMWDSTTKMAGAVADGLIS
                     QGIEVKAADLRGWHRSDVMAELLDSGAFIVGSPTLNNGYLPVVADLLCYARGLRPLGK
                     VGAAFGSYGWGGEAVKLLNEELEKMKVELVHQGVRVRYVPTPEDLENCRLMGVEVGRT
                     LRERLGS"
     gene            3426..4259
                     /locus_tag="PK146_03395"
     CDS             3426..4259
                     /locus_tag="PK146_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPE91318.1"
                     /translation="MKGSAPDLSCRRVAFLADRFEEMYRCYNRPEYIEPDPVSTVRVF
                     PDLIDREVAGFIASCLAYGRASQIVRSIRRVLGAMGENPGRFLLEADDQRVWELCKGF
                     RHRFTDSRDLFELLVSMRNSLKRWGSLEDLFASGVDGSPEKALRGLSRLSRALRSASG
                     ERKNTLLPDPSLGSACKRYHLFLKWMVREDAVDPGGWSCISPADLMVPLDTHMHRICT
                     VLGLVKRKNADVGAVVEATWSFRKICPTDPARYDFSLTRFGIRSDLEMKDLIGRCEDP
                     C"
     gene            4303..4968
                     /locus_tag="PK146_03400"
     CDS             4303..4968
                     /locus_tag="PK146_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91319.1"
                     /translation="MGILPYWDQNMDLEITPWTPYAKDYYPVTVNLLHSDQLGEIASL
                     ALGMDSELLFVESAETGRVGSLFRENLLIPGHGHHYEGGFSLIFGYSAMMEALLGMFT
                     QAFPGVRAGLAVCGVWDRFMLVDECKVIVLVGESLLLNESKCMRALQEVMSAATSDNG
                     SCSFFAEKFGYRRFSIPFETDDVTSLAISRMLFDFEMDATRALLGPPPEALLPEIFRE
                     HKL"
     gene            4965..5534
                     /locus_tag="PK146_03405"
     CDS             4965..5534
                     /locus_tag="PK146_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPE91320.1"
                     /translation="MIEPAVSVFLGFSLAMDALAVSVAMVVCSSLHFSAGPVIRVSGV
                     FGLFQALMPLAGWAFAGRALSIVGGVDHWIAFGLLSFVGGRMILEATRKREECLFPKD
                     PSRGLPLLLLAIGTSLDALAAGVSFMPLGLDPAFTSLVIGLVTFCTVAAGMNLANRIG
                     RRGGEKAAMVGGVILIGIGLKVLLSHLAG"
     gene            5656..>6483
                     /gene="panB"
                     /locus_tag="PK146_03410"
     CDS             5656..>6483
                     /gene="panB"
                     /locus_tag="PK146_03410"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPE91321.1"
                     /translation="MTKKKGRLEFIQMKQAGERAAWITAYDFPTASFAEGAGMDMILV
                     GDSLGMVVLGYEGTVPVTMDDCIRHSQAVRRGAPNTFCIGDMPFLSYQVSDEDAVRNA
                     GRFLKEADMDAVKLEGGRRVAGRIRAITDAGIQVMGHIGLTPQSSGQLGGFKAQGRDP
                     DSARELIKDALAVQEAGAYALLLEAVPPELTEYIWKKLEIPVYSIGAGAPCDGQLIIC
                     GDMLGLFQAFTPKFVKKYANVAEVEIAAFKEYVSDVKQGVFPADEHVYHILKGKEEDF
                     "
BASE COUNT         1483 a         1622 c         1925 g         1453 t
ORIGIN      
        1 gcgccctggc cggtcttttc ggggccgtag gtaccacctc ctacacggag aacatcgggc
       61 ttatcggact tactggtgtt gcctcccggt gggtggtgag gaccggagcg gtcattctta
      121 tcatcatgag catgatcggc aagcttggag ccctgatagc aacagttcca agtccagtca
      181 tcggaggcgc ctacatagcc ctcttcggga tcatcggtgc ccttgggatc caggtcctca
      241 tgagggccga catggggagc cagcgcaacg tcctgatcgt cgggttcgcc tttctcatgg
      301 ccctcgggct tcctggatgg accgaagggc agcaggaggc tttctttgct ttcggcattc
      361 cgggtcagat ggcctgggcc atcctgaaga cccccatggc tgtagcgggt atatgcgctg
      421 ccttctggga cagcattgtc cccggtacac ctgaagagag gggcattggc acaagactga
      481 aatagaaaca gatacacata cacatgaaag agattggggg ccttcggccc cctttttttg
      541 ttgacaaaat aaccgcaggg tgtaattata tcaatgtaat ctgtaaaggt ttcagaaata
      601 agccaggccg ttcaaaaact tgtctgagga aagtggtaaa ataagaatac ggaaaaggaa
      661 caataaatat ggaggtgtaa gtggaatggc aaagcagctg gatatcttca agtgtgaagt
      721 ctgcggcaat gtggtcgaac tgcttcacgt tggcggtggt gagctggtgt gctgcggtca
      781 gcccatgacc cttatggagg agaagaccgc ggattcggcg accgaaaaac acgttcccgt
      841 tatcgaaaag aaggacggag ggatccttgt aaaggtgggt agcataccgc acccgatgga
      901 ggacaagcac ttcatcgagt ggatccagtt gatcgttgac gggaagaact gcaagaaatt
      961 cctgaagcct ggagaggctc cggaagcttt cttctccgag aatggtgaca atgtatcggc
     1021 cagggaatac tgcaacatcc acggactctg gaggagctga ataagatgat caccaagaag
     1081 atgcagacag cgctgaacaa gcagatcaac gaggagcttt tctcctccta cctctatctt
     1141 tccatggctg ccgacttcga ggccagaaac ctcaccggtg ctgcgaagtg gatgaagatg
     1201 caggcagacg aagagcttga gcacgcaatg aaattcttcc actttgttct ggaaagagga
     1261 ggagaagtgg acctgctggc gatagccaaa ccgcagaaga gctggaagtc acctcttaca
     1321 gccttccagg atgcagaaaa gcatgagcag cacataacga agtgtatcaa cgacctttac
     1381 gaaatggcca ccaaggagaa ggattacgct acccagagca tgctcaagtg gttcgtggat
     1441 gagcaggttg aggaagaggc ttcggtcggc gagatcgtcg ccaggcttga gatgatagct
     1501 gattccaaga atggcctctt catgatggac agggagctgg gcaagaggga agaggactga
     1561 tcccccccct cccgtgaaga gaacggaaca tcaaggggga gcgggacctt ggtccccgtt
     1621 cccccttgat gatgtggata gtgttcccat gagatctttg gtaccatctg tccagacaag
     1681 agtggaggag gacggtcttc atggaagttg cgatcgacaa gaaggcactc tatgcccttt
     1741 cctacggact ctacgtcgtc agttcgagaa aagggaacaa gttgaacggc caggtcgcca
     1801 atgccgttat gcaggtcacg gcggaccccg tttccgttgt tgcctgcctg catcgcgata
     1861 acatgaccac cgaatgcatc agtgactccg ggatcttcag tgtctccatc cttgaagagg
     1921 atactccaat gacctttatc ggtaatttcg gtttcaagtg cggacgagat tatgacaagt
     1981 tcgggaagtg ttcgtacgaa ttgggggaaa caggggcacc tcttgtcatc gaccattcca
     2041 tagccgtcat tgaggcgagg gtagtcaaga atgtagatat tttcacccac gtcatgttct
     2101 ttggtgaagt ggtcgcctcc cgccacatcc gggaagggag acctctaacg tatgctcact
     2161 atcacgaggt caagaagggc aagtcgccaa agaacgcccc taccttcacc ctgaacgagg
     2221 tacgttgaaa aagggaggtg caaccatgaa cacgctggag ataatgaaag acgttcactg
     2281 ggtgggcgca atagactggg acctcaggga ctttcacggt tattccaccg agaaagggag
     2341 cacctacaac gccttcctcg ttgttgacga gaagacaacc ctgatggata cggtaaagaa
     2401 gaagcttgcc gacgagcttt tttcgaggat aagggaggtt aacccttcgg ggaggatcga
     2461 ttacctcgtc cttaaccatg ccgagatgga tcacaccggg agtctgaccg aggtgttcag
     2521 ggccttccgg ccggagaaga ttgtctgttc agcaccgtgc agggatgcaa ttattgaaca
     2581 cttcgggaaa aatgactggc cctttcacgt cgtccggacc ggtgacaccc tttcactcgg
     2641 caaacggacg atcagattca tcgagacgaa aatgctgcac tggccggaca gcatgttctc
     2701 ctaccttgaa gaggacaggc ttctgatctc cagcgatgct ttcggacagc acttcgcaac
     2761 gggagagcgt tttgacgacg aagttgatcc cctggagctg gtgaagcagg ccgcgaagta
     2821 ctatgcgaac atacttctcc cctattcagg acttgcggca aagctcctgg agagtatcaa
     2881 ggcaatgggt ctgaagatcg acatgatagc tccggatcac gggctgatct ggcgaaaagg
     2941 agtagccgac attctgaaag cttacggtga ctggtcctcc cagaaagcac tgccgaaggc
     3001 tgttgtagct tacagcacca tgtgggacag caccacaaag atggctggtg ctgtagctga
     3061 tggactgatc tcccagggga tagaggtgaa ggcggccgat ctcaggggtt ggcacaggag
     3121 cgatgtcatg gccgaactgc tcgattcggg agcctttata gtaggctcac cgacactgaa
     3181 caacggatat ctgccggtcg tggcggacct cctttgttac gccaggggct tgaggccact
     3241 gggcaaggta ggggctgcct tcggttccta cggctggggt ggagaggcag tgaagcttct
     3301 gaacgaggag cttgaaaaga tgaaggttga gctcgtccat cagggtgtca gggtcagata
     3361 cgttccgacg ccggaagacc tggaaaactg ccgacttatg ggagtcgagg tgggaaggac
     3421 ccttcgtgaa aggctcggct cctgatcttt cctgccggag ggtcgccttt ctggctgacc
     3481 ggttcgagga gatgtaccgg tgctataaca ggcccgagta catcgaacct gatcccgtct
     3541 caacggtaag ggttttccct gaccttatcg accgggaggt tgcaggtttt atcgcttcgt
     3601 gtctggctta cggcagggca tcccagatag tccggagcat caggagggtc ctcggggcaa
     3661 tgggcgagaa cccaggccgg ttccttttgg aagcagatga ccaaagggta tgggagctct
     3721 gcaagggctt caggcaccgt ttcaccgatt ccagagacct cttcgagctc cttgtttcaa
     3781 tgaggaattc cctgaagcgg tgggggtccc tggaggacct tttcgcttcg ggtgtggatg
     3841 gttcccccga gaaagccctc agggggctct ctcgcctctc ccgggcgctt cggtccgctt
     3901 caggtgaaag gaagaacacc cttctccctg acccttccct cgggagcgcc tgtaaaagat
     3961 atcatctttt tctcaagtgg atggtgagag aggatgcggt agacccaggc ggttggagct
     4021 gcatatcacc tgcagatcta atggtacccc tggacactca catgcacagg atctgcaccg
     4081 ttctgggtct cgtgaagagg aagaatgccg atgtcggtgc tgtggtagag gcgacctggt
     4141 cgttcaggaa gatatgtccc acggacccgg caagatacga cttttcgtta acccgtttcg
     4201 ggataagaag cgacctggag atgaaggatc tcataggccg ctgcgaagac ccttgttagg
     4261 ttataatcct cgttaaaaga aggacggagg gactcttcga gcatgggtat tctaccctat
     4321 tgggaccaga atatggatct tgagataaca ccgtggacac cctacgcaaa ggattactac
     4381 cccgttacgg tcaacctgct tcacagcgat cagcttgggg agatcgcatc cctcgccctt
     4441 gggatggaca gtgagctgct cttcgtggag agtgcggaga cgggtcgggt gggttcgctt
     4501 ttcagggaaa atctcctgat acccgggcac ggtcatcact acgaaggagg attctccttg
     4561 atcttcggct attctgccat gatggaggcc ctcctgggca tgttcacaca ggccttcccg
     4621 ggggtcaggg ccggtctggc cgtatgcggt gtatgggaca ggttcatgct tgtcgacgag
     4681 tgcaaggtaa ttgttctcgt gggagaaagc ctcctgctta acgaaagcaa gtgtatgaga
     4741 gccctccagg aggtaatgtc cgccgcaacc tccgataacg ggtcctgctc cttctttgcg
     4801 gaaaagttcg gttaccggcg cttctcgatc cccttcgaga ccgatgacgt aacgagcctg
     4861 gcaatttcta ggatgctttt cgacttcgaa atggatgcca cccgtgcctt gttgggtccc
     4921 cctcccgaag ctctgctccc ggagatattc agggagcaca agctttgatc gagccagctg
     4981 tttcagtatt tcttggcttc agccttgcca tggacgctct tgcggtctct gtcgccatgg
     5041 tggtctgctc ttccctgcac ttctcagccg gaccggtgat tagagtctcc ggtgtcttcg
     5101 ggctcttcca ggccctgatg ccccttgccg gatgggcctt tgctggaaga gccctttcca
     5161 tagttggagg cgtggaccac tggatagcct tcggacttct ttcctttgtg gggggcagga
     5221 tgatccttga agcgaccagg aagagggagg agtgcctttt ccccaaagat ccctcccggg
     5281 gtcttccgct tcttctcctg gcgataggga caagccttga tgcgcttgcg gccggggtaa
     5341 gcttcatgcc tctcggcctg gatcctgctt tcacttcctt ggtgataggc cttgtaacat
     5401 tctgtactgt ggctgcaggc atgaaccttg cgaaccggat cgggagaagg ggcggagaga
     5461 aggctgcgat ggtaggaggg gtgatcctta tcggcattgg tctgaaggtc ctgttatcgc
     5521 accttgcagg ataatatccg ggtgaaaagg gatatcataa gagaataaga cagcctttca
     5581 gaatgttgtc caagaggtct tttgatacta ctctatatgg gccgatcaaa tcaattttgg
     5641 gaaggagctg ttccaatgac caagaagaaa ggaagacttg agttcatcca gatgaagcag
     5701 gcgggcgaac gggccgcctg gatcactgcc tatgacttcc cgacggcttc ctttgcagaa
     5761 ggagcgggga tggacatgat cctcgtcggg gattcgctgg gtatggtggt cctggggtac
     5821 gaaggtaccg ttccggtcac gatggatgac tgtatccgcc actcccaggc tgtccgcaga
     5881 ggtgctccca acaccttctg tattggtgac atgccctttt tgagctacca ggtaagcgat
     5941 gaggacgctg tccgcaatgc cggccgtttc ctgaaggaag ctgacatgga tgccgtcaag
     6001 ctggagggtg gacggcgtgt tgctggccgc ataagagcca tcaccgacgc agggatccag
     6061 gtcatgggac acatcggtct caccccccag agctccggcc agctgggcgg tttcaaggcc
     6121 caggggcgcg atccggacag tgccagggag ctgatcaagg atgctttggc cgtacaggag
     6181 gcgggagcct atgccctgct tctggaggct gtcccgccgg agctgacgga gtacatctgg
     6241 aaaaagctgg agattcccgt ctattcgatc ggtgccggtg cgccctgcga cggtcagctg
     6301 atcatatgcg gagacatgct ggggctattc caggccttca cccccaagtt tgtaaagaaa
     6361 tacgccaatg tcgccgaggt tgagatagca gccttcaagg agtacgtatc tgacgtaaaa
     6421 cagggtgttt tcccagccga cgagcatgta taccatatcc tgaagggcaa ggaagaggat
     6481 ttc
//
LOCUS       DAOPKQ010000064         6396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_12959_length_6396_cov_4.276770, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000064 DAOPKQ010000000
VERSION     DAOPKQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12959_length_6396_cov_4.276770"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /gene="pyk"
                     /locus_tag="PK146_03415"
     CDS             complement(<1..1225)
                     /gene="pyk"
                     /locus_tag="PK146_03415"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPE91322.1"
                     /translation="MKKRAKIVCTLGPACEEYEILKRMVQAGMDVARLNFSHGDHHTH
                     EKHLDRVRRLEKELDRPIGTMIDTKGPEIRTGTLRGRMPVVIESGKTIILTSEPVEEG
                     DASRVWISHPGLSGEVARGQALFIDDGTLHLTVEKVAGKDIICRVNVGGELGERKGIS
                     VPGAKISLPILTPRDVDDVRWALEKKMDLIALSFVRDRSDIMEVRRVLEEIGGNLRII
                     AKIETREAVDNIEEIIEVVDGMMVARGDLGVEIPLEEVPLVQKMIIDRCRLHGKPVIV
                     ATQMLDSMIRNVRATRAEASDVANAVLDGADALMLSGETANGAHPVLSVETMSRIIRK
                     TEEGESPWRRAIPLQTGAPGIPDAVSKAAGMISDQMKSQAVLSLTQSGVTAQMVSKYR
                     PGAPIIGATPHQETLR"
     gene            complement(1209..1469)
                     /gene="mtrB"
                     /locus_tag="PK146_03420"
     CDS             complement(1209..1469)
                     /gene="mtrB"
                     /locus_tag="PK146_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HPE91323.1"
                     /translation="MRVENNGKPSVLSDFIVVKALENGVSVIGMTRGQETKFAHSEKL
                     DAGEVWIAQFTEFTSAMKIRGKALILTAHGAVESGKRNEETS"
     gene            complement(1517..4987)
                     /gene="dnaE"
                     /locus_tag="PK146_03425"
     CDS             complement(1517..4987)
                     /gene="dnaE"
                     /locus_tag="PK146_03425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPE91324.1"
                     /translation="MNRPFVHLHVHSEYSLLDGAIRCCDLARRSAQWGCPAVSITDHG
                     ALYGAIEFYSACREEGVKPVIGCELYVDPRGHTLKDRMGRNYHLLVLAENQEGYQNLV
                     KLVSIANTDGFYYRPRVDHDLLSRYSGGLIASSACLAGEIPSLILEGAEDEALQRAML
                     YRDIFGEDNFFLEIMHNGIPEQAAANRALIKMSSRTGIPLIATNDAHYLEAGDASWHD
                     VLLCVQTNSTVNTPNRYRFGADDFYLRSPEEMWSLFGKDVPQALENTVAIAERCNVDF
                     TFGAYRLPRVEVRKGTTLEEELHIRSHEGLKARFGELSVPREYLERLDYELNVINQMG
                     FAGYFLIVSDIIHSAKEESIPVGPGRGSAAGSLVAFSLRITEIDPLRYGLLFERFLNP
                     ERISMPDIDTDISDRGRDRVLEIIVEKYGRSHVAQIITFDRMKSRAAIRDVGRALDMK
                     YPDVDRIAKLVPSGAVSIEEAVEQSPDLKQAKKEDPSVGKLLDYASRIEGLARHCSQH
                     AAGIVITPEPLTEVVPVRRIGEAQIVTQYSMEPLEKLGLVKMDFLGLRTLSMIEETLS
                     NITRNGKKAPDVYSIPPDDQPAFDMLCRADTLGVFQLESSGMRQLLKQLRPDCFEDLI
                     AVLALYRPGPLGSGMVEQYIDCKHGRRDISWLHEKLKDVLKETYGMILYQEQVMECAA
                     RLSVSSKPRRALQEQVMECAARLAGYTLGEADLLRRAMGKKKADVMETQRKQFVEGCV
                     GSGVSHSDAEHIFDLVQEFAGYGFNKSHSAAYAMITYQTAFLKAHFPSEFYAAFLSSH
                     IHSKLDTLAGHIRAVRNSGIKVLPPDVNRSEANFSVDGDVILFGMSAVSKLGDPTVVH
                     IIGVREKGPFLSLWDFINRVDTRVVGRAVMENLVRAGAFDSITSNRRQLMESLPGMFE
                     LAQRRASDCRSQQLLGLEDLAEEEGPVLLEVEDYDLHTRMEMEKEATGLYISGHPFDQ
                     YEKEVQPYSTCSIPDLACWKAENCPAVVAGILVGIQEKFTRKGDRMGILEIEDSSGKI
                     EVVCFPRTWSGITLPEKGKLVIVKGAPRTREGLSLIAEKIMTVEELKGNARRWLRLRI
                     LPDGPALNGRLREVYRELKGHPGDASVLLEVAVGGKKAILRARDLNVEPSQDLSAKIM
                     EISGGGIEVV"
     gene            complement(4987..>6396)
                     /locus_tag="PK146_03430"
     CDS             complement(4987..>6396)
                     /locus_tag="PK146_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91325.1"
                     /translation="EVHMLTDPAFNALLKTLEEPPQTVVFILATTEPHKVPATIRSRC
                     QHIPFHRIPVPAIVECMEKVSALEGAVIEQDALWEMARESDGSLRDALSLLEQAVTSG
                     LPAVSLEDIRGILGGGGRSDLERLIPAMRDGSAEVFRGFSELLQRGLTAERLLEGLFL
                     LFRDILVTVLWGEDGISSLPLSEDERTFIREESGHWREPELWKVLNFCSATLPRARYG
                     LKAEVVLGMVLGLFVLSPAKTEERVRKEAAFEMEDGATPGKGRSHKIEQKPEQKPEQK
                     EHEIHSPDKEEVLQGSPPASDVSSGGNWDECVDAMASDNLPLYCACVPTRSERDGDSL
                     VILFPEDRQFSFEIASSPRSIAYLDSIRLDRFPGVQMKLVCGDRSVQVEDNFSAGNAV
                     QPGEQDGSPNTFSSSGDVPSEKSPPSVRVRANHIETEDGEQRQECFLEIQKLFNADLL
                     VLKEENPDEPDNPEGAVAE"
BASE COUNT         1223 a         1979 c         1725 g         1469 t
ORIGIN      
        1 acctcaatgt ctcctggtgg ggtgttgccc caattatggg ggctcctggc ctgtatttgc
       61 tgaccatctg ggccgtaacc ccgctctggg tcagagagag gacagcctgg gacttcatct
      121 ggtcggagat cattcccgcg gccttgctga ctgcgtcggg tatccccgga gctccggtct
      181 ggagcgggat agcccttctc cagggagact ccccctcctc ggttttcctg atgatccttg
      241 acatggtctc caccgacagc acaggatgag cgccattggc cgtctcgcca gagagcatga
      301 gggcatccgc tccgtcaaga acagcattgg caacatcgct tgcttcggcc ctggtggccc
      361 tcacgttcct tatcatcgaa tccagcatct gggtagcgac aatgacgggc tttccgtgga
      421 ggcggcacct gtcgatgatc atcttctgca ccagggggac ttcctccagg ggtatctcca
      481 cccccagatc gcccctggcc accatcatgc cgtccactac ctcgattatc tcctctatgt
      541 tatccactgc ctcgcgggtc tcgatctttg cgattatcct caggttgccc cctatctcct
      601 cgagcacccg gcggacctcc atgatgtcgg acctgtcgcg gacaaaggag agggctatca
      661 ggtccatctt cttctccagg gcccaacgga catcatcgac atcacgggga gttaggatgg
      721 ggagcgaaat cttcgctcca ggtacggaga ttcccttcct ttcgcccagt tcaccgccga
      781 cgttgactcg gcagatgatg tccttccctg caactttttc gacagtgagg tgaagggtgc
      841 cgtcgtctat gaagagggcc tgcccccggg cgacctcacc ggacagaccc gggtggctta
      901 tccagaccct ggatgcgtcc ccctcctcga caggttctga ggtaaggata atggtcttgc
      961 cgctctcgat cacgaccggc atacgtcccc tgagggtccc ggtcctgatc tcgggtccct
     1021 tggtgtctat catggtccct atcggcctgt ccagctcctt ctccagcctc ctgaccctgt
     1081 caaggtgctt ttcatgggta tggtgatccc cgtgactaaa gttcaggcgg gctacatcca
     1141 tgcccgcctg aaccatcctt ttcagaatct cgtattcttc gcaggccggc ccaagggtac
     1201 agacgatctt agctcgtttc ttcattcctt ttcccgcttt cgacagcccc gtgggctgta
     1261 agaattaggg cctttcccct gatcttcatg gctgaggtga actcagtgaa ctgggctatc
     1321 cagacctcac cggcatcaag cttctccgag tgggcgaact tcgtctcctg acctctcgtc
     1381 atacctatca ccgagacccc gttctccaga gccttaacga caatgaaatc agaaaggacc
     1441 gaaggcttac cgttattctc cacgcgcatt tcctctcctc ctcagcgccc ttgacaaagg
     1501 ggctacctac cctattttat accacctcga tacctccgcc ggaaatctcc attatctttg
     1561 cggaaagatc ttgggagggt tcaacgttaa ggtccctggc cctcagtatc gcttttttcc
     1621 ctccaacagc tacttccagg agcacgctgg catcccctgg gtgccccttg agctcccggt
     1681 acacttctct aagcctaccg ttaagggccg gaccgtcggg aaggatcctg agccggagcc
     1741 accttctggc gttgcccttc agctcctcga cggtcattat cttttctgcg atcagcgaca
     1801 gtccttccct tgtcctgggt gcccctttga caatgacaag ctttcccttc tccggaagtg
     1861 ttatgcccga ccaggtcctc gggaaacaga ccacctctat cttccctgaa gagtcctcga
     1921 tctccaggat gcccatgcgg tcacccttcc tggtgaactt ctcctggatc cccaccagta
     1981 tcccggccac aacggcaggg cagttctcag ccttccagca tgccaggtcc ggaatggaac
     2041 aggtgctgta tggctgaact tccttctcgt actgatcgaa ggggtgtccc gaaatataga
     2101 gaccggtggc ctccttctcc atctccatcc tggtatggag gtcgtagtcc tccacctcca
     2161 gcaggaccgg gccctcctcc tcggcaaggt cttcaaggcc cagcaactgc tggctgcggc
     2221 agtccgatgc ccttctctgg gcaagttcga acatccccgg gagggactcc atgagctgac
     2281 gcctgttgga ggttatggaa tcgaaggcac ccgcccttac aaggttctcc atgactgctc
     2341 ttcctacgac cctggtgtcg acacggttta tgaaatccca gagagacagg aagggcccct
     2401 tctcccttac cccgataata tggaccaccg tcggatcccc cagcttcgag actgcgctca
     2461 taccgaacag gatcacgtcc ccgtcaacgc tgaagttggc ctcggacctg ttgacgtcgg
     2521 gaggcagtac ctttattccg gagttcctta cagcccttat gtgtcctgcc agggtatcca
     2581 gtttggagtg aatgtggctg gagaggaagg ccgcatagaa ttcgctgggg aaatgggcct
     2641 tcaggaaggc cgtctggtaa gtgatcatcg cataggcagc gctgtgggat ttattgaaac
     2701 cgtagccggc gaactcctgg accaggtcga agatatgctc cgcgtcggag tgactgaccc
     2761 cgctaccaac gcacccctcc acgaactgct tcctctgggt ctccatgaca tcggccttct
     2821 tcttgcccat ggccctgcgg aggaggtcgg cctcaccgag agtgtatcca gcaagcctcg
     2881 ccgcgcactc catgacctgc tcctggagtg cgcggcgagg cttgctggat acactcagcc
     2941 tcgccgcgca ctccatgacc tgctcctggt agaggatcat gccgtaggtc tccttaagga
     3001 catccttcag cttttcgtgg agccaggata tatcccttct cccatgcttg cagtctatgt
     3061 actgctccac cattccgctg ccaaggggac cgggccggta gagagcaagc acggctatca
     3121 gatcctcgaa acagtcaggg cgaagctgtt taaggagttg cctcatgcct gaggattcca
     3181 gctggaagac ccccagcgtg tcggcacggc agagcatgtc gaaggcaggc tggtcgtcgg
     3241 gggggatcga gtaaacatcc ggggccttct tgccgttgcg ggtgatgttc gacagggtct
     3301 cctcgatcat tgacagggtc ctgagaccca gaaaatccat ctttacaaga ccgagcttct
     3361 ccaggggctc catggagtac tgggtcacta tctgagcctc gcctatcctt cgaacaggaa
     3421 caacttccgt gagcggctcc ggggtgatca ctattcctgc tgcatgctgg gagcagtgtc
     3481 tcgcgagccc ctctatcctt gacgcgtaat cgagcagttt gcccaccgaa ggatcttcct
     3541 tctttgcctg cttcaggtca ggtgactgct ccacggcctc ctcgatggag accgctcctg
     3601 agggaacaag cttggcgatc ctgtccacgt cggggtactt catgtccagg gcccttccta
     3661 catcccggat ggcagccctg ctcttcatcc ggtcaaaggt tattatctgc gccacgtggg
     3721 atcgtccgta cttctccaca atgatctcaa ggacgcggtc ccgccccctg tccgatatgt
     3781 ccgtgtctat atcgggcatg cttatcctct ccggattgag gaacctttca aagagcaggc
     3841 cgtaccttag ggggtcgatc tcagtgatcc tgagagagaa ggccaccagg gaacccgcag
     3901 cagaacctct ccccgggccg acggggatcg actcctcctt tgcggagtgg atgatgtcgg
     3961 agacgatcag gaagtaaccg gcgaacccca tctggttgat cacgttcagt tcatagtcca
     4021 gcctctcgag atactccctg gggacagaca gctcgccgaa acgggccttt agcccctcat
     4081 gggaccttat atggagctcc tcctcgaggg ttgtcccctt tcggacctca accctgggca
     4141 gtctgtaggc cccgaaggtg aagtccacgt tgcaccgttc cgctattgcc acggtgttct
     4201 ccagtgcctg tgggacatct ttgccaaaga gggaccacat ctcctccggt gagcgcaaat
     4261 agaaatcgtc ggccccgaaa cggtacctgt taggggtgtt cactgtagag ttcgtctgga
     4321 cacagagcag aacgtcgtgc caggaggcat ccccggcctc caggtagtgg gcgtcgttgg
     4381 tcgctatcag ggggatgccc gtccgggagg acatctttat cagggccctg ttcgctgccg
     4441 cctgttcggg gatcccgttg tgcattatct cgaggaagaa gttgtcctcg ccgaagatgt
     4501 cacggtacag catggccctc tggagagctt catcttccgc cccttcaagg atgaggctgg
     4561 gtatctctcc ggcaagacag gccgaactcg ctataaggcc gcctgaataa cgggagagaa
     4621 gatcgtgatc gacccggggg cggtagtaga atccgtctgt gttggcgatg gagacgagct
     4681 tgaccaggtt ctggtaaccc tcctggttct ctgccaggac caaaagatgg tagttccttc
     4741 ccatgcggtc ctttaaggtg tgccccctgg gatccacgta gagttcgcac cctatgacgg
     4801 gtttgacccc ctcctctcgg caggccgaat aaaactcgat ggcaccgtac agagctccgt
     4861 ggtcggttat agatacggct ggacagcccc actgtgcgga gcgccttgcc aggtcgcagc
     4921 acctgatcgc tccgtccaga aggctgtatt cgctatggac atgaagatgg acaaagggtc
     4981 tgttcactat tctgctactg ctccttccgg gttgtctggt tcatcggggt tctcttcctt
     5041 cagcacgagc aggtcggcat tgaataactt ctggatctcc aggaagcact cctgcctttg
     5101 ttctccgtcc tcggtctcga tatggtttgc ccgcaccctc accgaggggg gcgacttttc
     5161 ggaagggaca tctccggagg aagagaaggt gttagggctg ccatcctgct ctcccggctg
     5221 gacagcattt ccagcggaaa aattatcttc aacctggacc gacctatccc cgcagaccag
     5281 cttcatctgc acccctggga aacggtctag ccttatggag tcaaggtagg cgatgcttct
     5341 cggtgaggaa gctatctcga aggagaactg ccggtcctcc gggaacagga tgaccagcga
     5401 gtccccgtcc ctttctgacc ttgtggggac acaggcacag tagagcggca ggttgtcaga
     5461 tgccatggcg tcgacacact catcccagtt accaccggac gacacgtcag atgccggggg
     5521 agacccttgc aggacctctt ctttgtcggg agaatggatc tcgtgctctt tctgttcagg
     5581 tttctgttca ggtttctgtt cgatcttatg gctacgcccc ttcccggggg tagctccatc
     5641 ttccatttcg aatgccgctt ccttccgaac cctctcttcc gtcttcgccg gcgagagcac
     5701 gaaaagcccc aaaaccatgc ccaggaccac ctcggccttc agtccgtaac gggcccttgg
     5761 aagtgtggca gaacagaagt tgaggacctt ccagagctcg ggttctctcc agtgccccga
     5821 ttcttcccgg atgaaggttc tctcatcttc gctcagggga agtgagctga tcccatcctc
     5881 gccccagaga acagtcacga ggatgtccct gaaaagaagg aacaggccct caagtaaccg
     5941 ttcagccgta agtccccgtt gaagaagctc cgagaaaccc ctgaacacct cggcgctccc
     6001 gtccctcatg gcagggatca gtctctccag gtcgctcctt cctccgccgc cgagaatgcc
     6061 ccggatatcc tcgagagaca cagccggaag acctgacgtt actgcctgtt ccagcaggga
     6121 gagagcatcc ctcagtgatc cgtccgactc ccgggccatc tcccagaggg catcctgctc
     6181 gatcacggct ccctcaaggg ctgagacctt ctccatgcac tcgacgatgg caggcacggg
     6241 aatgcgatgg aaggggatgt gctggcacct ggagcggatc gtcgcaggga ccttgtgcgg
     6301 ctctgtcgtt gcaagaatga agactaccgt ctggggaggc tcttccaggg tcttcagaag
     6361 ggcgttgaaa gcagggtcgg tgagcatgtg cacctc
//
LOCUS       DAOPKQ010000065         6377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_13017_length_6377_cov_5.185226, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000065 DAOPKQ010000000
VERSION     DAOPKQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13017_length_6377_cov_5.185226"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK146_03435"
     CDS             complement(<1..538)
                     /locus_tag="PK146_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91326.1"
                     /translation="MTTVCSWLRDRELEGICSEILALITMPLIAGSVTEEHLVVEFSN
                     SYFDELTGLSSLDLPETLDVLLSGIEGPRDLEPLYLAAREKGWKTVRWTLVPGEIELE
                     LYCWSGDKHFLAFFHDLSPHEFHARLLVMKERFTQLIGLYDVKGMIPQMILELVDHMG
                     VDWAGIFVWDGARERWMLS"
     gene            628..1410
                     /locus_tag="PK146_03440"
     CDS             628..1410
                     /locus_tag="PK146_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPE91327.1"
                     /translation="MDLETKKIDVPGTFGDGLVPVHIMRRSGASGGSPLVVLLHGVHG
                     SANPLPGNKYGELARMLIEQGASCAIVETSRIRRDRESFGEDREAWAHAAFKGKTFFQ
                     DHADAVAGIEAAAMEAGIGSIWVFGFSLGGIHAVLAAGSEGLSFTPSGIALGGSGYRI
                     RSEAENALTLPILDSMPGSDPLIEAAGRLRAERLVSFYGSLDSTFPEDTCRRLVELAP
                     LPLEKKGFVVIEGADHAFRSLKGNPSLKPLEMISSFLTPLLF"
     gene            complement(1407..2165)
                     /locus_tag="PK146_03445"
     CDS             complement(1407..2165)
                     /locus_tag="PK146_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPE91328.1"
                     /translation="MIVFSALLGACMGSFLNVVASRTVEERSWWGGERSRCDSCGREL
                     TALELIPIVSWFALSGGCRSCGAKIPLGYLLVEVGGAAIGGLIAWRWGVSVTSAFAAV
                     VAAGLFLNALTDLYSGFVYDLFAWGLGAVGMIMRIGGGWGALLDGLLGAGLGFAVIAV
                     IIFVSRGGMGWGDASLMAGAGAALGWQLSAWGLYSGFMIGGVIALGLLLFRRVRRKDA
                     IPLGPFLAVGGIVSMLTGTWFYSFLGMSVGWPWG"
     gene            complement(2277..2717)
                     /locus_tag="PK146_03450"
     CDS             complement(2277..2717)
                     /locus_tag="PK146_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91329.1"
                     /translation="MQMPMQISLDEVLSMLLARVDSLAFNDENLKTKFNILARAMYRK
                     GLITDDDVIESIREEHRILMDLGVIQEMPTEDVIQTVAEGILQWIKGDVEAIKKAMDD
                     YEKKLQELSKQQQAKPKIDVASPAVLQQLDKLSNQGKGKSKLIY"
     gene            2815..3702
                     /locus_tag="PK146_03455"
     CDS             2815..3702
                     /locus_tag="PK146_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPE91330.1"
                     /translation="MKWSLRKTILRTLSATRKELPTFALMTFGTLIVAFGIMSLTIPY
                     RLPDSGVSGIAVLTNYAFGISPAWVVGVANIILLAWGMKELSVRFVGWTVYAVTLLTA
                     LLKLFEGIPYPHLDELLLVAILAGAVKGLGGGLVLRSGSSLGGTDILVVALRKRYGIE
                     VGRFTFYINLVILGVSVFIVGLEGAIYGLVSIFVNGVVLDNVLRSFDRRRQVFVISNH
                     PKEIADFITGELHRGVTILHGEGGFTHEDRPALLCVLTPRQTMDLKRFLSVNDPKAFM
                     IVSDASEVLGRGFKSWKEL"
     gene            complement(4026..5018)
                     /locus_tag="PK146_03460"
     CDS             complement(4026..5018)
                     /locus_tag="PK146_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91331.1"
                     /translation="MLSLVRGGTREIFLKLKDQGVIESVAAKFPLVEKQYEDAWDSSS
                     EGQTLIFATMKGRSLGEIRVFLADSSPGEILAFMLNEGLCDKLEKASMLPRTILFRVS
                     GDYSEVMEQMKQDLDAKVGKIPLFLRWTDSGGRVAVMFTRNNLNRPIAIKDLFPDVLY
                     IQMPYEQLLRSLRSRAMSYFNEARGHADWRSLEIRVYDMWERYPLQAKRLRLVLDELE
                     IGLVLGEGRGKDFAHILMPVPVYRFHLATFLEPERIKEILMGMEYSASGKRLVDLDLF
                     EGKKKTSWGAMAEKDEDRERAGVRLRERLMEGLLPSTGDQLARIEEEIEGEERA"
     gene            complement(5038..5652)
                     /locus_tag="PK146_03465"
     CDS             complement(5038..5652)
                     /locus_tag="PK146_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPE91332.1"
                     /translation="MGAWKDFLACFRTVLPLPLHLQEARGKCLVHVSDTPSVFYGDLR
                     RLLEFLEPLCLVHTGDLVDDVKLALRPGYSRIFRNRLRLLAEVMSAVPGDRVAIITGN
                     HDLPEVVRSAFPGVMVFEEGIRLSLCGLDVALAHSLEELPLPPAAFNLFGHDVEVPLD
                     HEGSLYLNGVNGINVILLEGGTVEVLPYPPYVDDARCCRRRTGL"
     gene            complement(5668..6141)
                     /locus_tag="PK146_03470"
     CDS             complement(5668..6141)
                     /locus_tag="PK146_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPE91333.1"
                     /translation="MAKPNKALEKEIMAILEKNLKSIWEGDVAAYSNTTSEDCTFFEW
                     YISPQRIDGLEFHLRELRVHRMVVGGSPDPLEEEEQLFEHEILQPKVQVFGATAVVTF
                     TLMVRAVFPGKIVHKSHNETRVFNNFGTKKNPDWKLVHCHKSPMVTPDSMSVLRS"
BASE COUNT         1342 a         2002 c         1691 g         1342 t
ORIGIN      
        1 gggagagcat ccatctctcc cgtgccccgt cccatacgaa tattccggcc cagtccacac
       61 ccatgtggtc caccagctcc aggatcatct gtgggatcat gcccttcacg tcgtagagcc
      121 ctatgagctg ggtgaatctc tccttcatga ccagaagcct ggcgtggaac tcgtgggggg
      181 acagatcgtg gaaaaaggcg aggaaatgct tgtccccgct ccagcagtag agttcgagct
      241 cgatctctcc cggcacaaga gtccacctta cggtcttcca gcctttctcc ctggccgcca
      301 ggtaaagggg ctccaggtcc ctcggtccct cgatccccga caacaggaca tccagcgtct
      361 ctggaagatc caggctgctc aggccggtaa gttcatcgaa gtagctgttc gagaactcca
      421 ccaccaggtg ttcctctgtc acgctccctg cgatgagcgg catcgtgatc agggcaagta
      481 tctcagagca gatcccttcc agttctctat ccctgagcca gctgcagacg gtggtcatcg
      541 gtcaatgccc ccccgaggta agtgctgcat ctcttcccta tgataaactt aatgtgctca
      601 aagagccata ccgggaggag gggccccttg gatctcgaga caaaaaagat agatgtaccc
      661 ggcaccttcg gggacggcct ggtccctgtc catatcatga gacggtccgg ggcctccggc
      721 ggctcgcccc tggtcgttct cctgcacgga gtccacggct ccgccaaccc cctgccgggg
      781 aacaagtacg gcgaactggc acgaatgctg atcgagcagg gagcgagctg tgccatagtc
      841 gagacaagcc gcatcaggcg cgaccgggag agcttcggcg aggaccggga ggcatgggcc
      901 catgcggcct tcaagggcaa gacattcttc caggaccatg ccgatgccgt agcaggtatc
      961 gaggcggccg ccatggaggc cggcataggc tccatatggg tcttcggatt ctcccttggg
     1021 gggatacacg cagtccttgc cgccggaagt gaaggccttt ccttcacacc gtccgggata
     1081 gccctggggg gatcgggtta caggatacgc tccgaggcag agaatgccct gacacttccc
     1141 attcttgact caatgcctgg tagcgacccc ctcatcgagg ccgccggcag actgagagcg
     1201 gagagacttg tctctttcta cggttcgctg gactccacat tcccggagga cacctgtcgc
     1261 agactggtgg agctggcacc cctgcccctg gagaagaagg gattcgtagt tatcgaggga
     1321 gctgaccatg ctttccgaag cctgaagggt aacccctcac tgaaaccact ggagatgatc
     1381 tcctccttcc tgacccctct cctcttctaa ccccagggcc aacctacaga catcccgagg
     1441 aaggagtaga accaggttcc ggtcagcatc gagacgatcc ctccgacggc caggaacggc
     1501 ccgagcggta tggcgtcctt tcgcctgacc ctgcggaaca ggagcagacc cagggcaatt
     1561 acccctccga tcatgaaacc cgagtagagg ccccatgcgg acagctgcca ccccagggcg
     1621 gcgcctgcac ctgccatcag gctggcatcg ccccacccca tgccccccct gctcacaaag
     1681 attatcacgg cgatcacggc aaagcccagc ccggccccga gcaggccgtc aagcagggca
     1741 ccccagcccc cgccgatccg catgatcata cccacagctc caagtcccca tgcaaagagg
     1801 tcgtagacaa atccgctgta aaggtccgtc agggcgttaa ggaagagacc cgctgcgacc
     1861 accgccgcga aggcggaagt gacagacacc ccccacctcc aggctatgag gccaccgatg
     1921 gccgctccgc cgacctcaac cagcaggtat cccaggggga tcttagcccc gcatgacctg
     1981 cacccgccgg agagggcgaa ccatgaaact atcggtatca gttcgagggc ggtcagctcc
     2041 cttccacagc tgtcgcacct cgaacgctcg ccaccccacc atgaacgctc ctccactgtc
     2101 ctggaggcga caacgttcag gaaggacccc atgcaagccc ccagaagggc cgaaaaaaca
     2161 atcaaaatgg aagaaaaggc tacgggcact atccccacca ctccttgccg cggaggatga
     2221 actctacgaa aaagggtccc ggcttaagcc gggaccctcc aattgtatct cgtgaactag
     2281 tagatcagct tgctcttccc cttgccctgg ttactgagct tgtccagttg ctgaagcacc
     2341 gccggcgagg ccacatctat cttcggtttc gcctgctgct gctttgacag ctcctggagc
     2401 ttcttctcat agtcgtccat ggcctttttg atggcctcca catcgccctt gatccactgg
     2461 agaattccct ctgcaacggt ttggatcacg tcttcggtcg gcatctcctg tatcacgcca
     2521 aggtccatca ggatccggtg ctcctcgcgg atagattcga taacgtcatc atcggtgatc
     2581 aggcccttgc ggtacatggc cctggcaagg atattgaact tggtcttgag gttctcatcg
     2641 ttgaaggcca ggctgtcgac cctggccagg agcatggaca ggacctcgtc cagacttatc
     2701 tgcattggca tctgcatctt gtaattttgc ccccttttct gagatcggca acaattgtaa
     2761 cacgaagcag gccccctctt tgaaggcggc cacggaggaa ttatactctg ttacatgaaa
     2821 tggagcctca ggaagaccat acttcgcaca ctgtccgcaa caagaaagga actgccgacg
     2881 ttcgcactga tgacgttcgg caccttgatc gtggccttcg gcatcatgtc cctcaccatc
     2941 ccctatcgcc tccccgactc gggcgtatca gggatcgccg tactcaccaa ctacgctttc
     3001 ggcatatctc cggcctgggt ggtgggagtg gccaacatca tccttcttgc ctgggggatg
     3061 aaggaactat cggtacgttt cgtagggtgg accgtctacg ccgtgaccct cctgacggcc
     3121 cttctcaaat tgttcgaagg tataccctac ccgcacctgg acgaacttct tctggtggcc
     3181 atcctggccg gggcagtaaa ggggcttggc gggggacttg ttctgcgttc gggaagctcc
     3241 ctgggcggca cggacatact ggtggtggct ctcaggaagc gttacggcat agaggtcggc
     3301 cgtttcacct tctacatcaa cctggtcatc ctcggagtat cggtcttcat cgtcgggctc
     3361 gagggagcca tctatggcct ggtaagcata ttcgtcaacg gagtggtcct cgacaacgtc
     3421 ctccggtcct ttgacaggag gaggcaggtc ttcgtcatca gcaaccaccc gaaagagatc
     3481 gccgatttca ttaccgggga actccaccgg ggcgtcacga tccttcacgg tgaaggaggg
     3541 ttcacccacg aggaccgtcc cgcccttctc tgcgttctga caccccgaca gaccatggac
     3601 ctgaaaaggt tcctctccgt caacgacccc aaggccttca tgatagtatc cgacgcttct
     3661 gaggtcctcg gcaggggctt caagagctgg aaggagcttt aaaagaacaa ggcgacagac
     3721 ttcaggaggc aggcaacagg aaaacccggg gccaaccctt ctcccttcaa actggttctc
     3781 cttggcttcc cccattaaaa aagattctcc tctgtggccc caccctaccc tacgctggcg
     3841 gtacgccagc tagggcagag cgaagcctac cgacatagtc ccgaggagtt ttaacgaggg
     3901 aggtgatgtg tctgtgtaac tctgtgtcca ctgtggtgag atgccctggc tttggggtgg
     3961 gtattaccgt tcactgttta ccgcttgccg ttcacgcttg tgaatttact tttacatctc
     4021 acgtcttacg ccctctcctc cccctcgatc tcctcttcta ttcgggcgag ctgatccccg
     4081 gtggagggaa gcagtccctc catcagccgc tcccgcagtc tgaccccggc cctctccctg
     4141 tcctcgtcct tttctgccat ggcaccccag ctggtcttct tcttgccctc gaagagatcc
     4201 aggtccacca ggcgcttccc ggaggcgctg tactccatcc ccatcaggat ctccttgatc
     4261 ctctcgggct caaggaaagt cgccagatgg aaccggtaga cgggaacggg catcagaata
     4321 tgggcgaaat ccttccccct gccctcgccc aggacaaggc cgatctccag ctcgtccagt
     4381 accaggcgga gtcttttagc ctgaagaggg tacctctccc acatatcgta gacccggatc
     4441 tccaggcttc tccagtcggc atgcccccgg gcctcattga agtaggacat ggccctgctc
     4501 ctcagactcc tcaaaagctg ttcgtagggc atctggatgt agagaacgtc ggggaagaga
     4561 tcctttatcg ctatggggcg attgaggttg ttcctggtga acattactgc caccctgccg
     4621 ccgctatcgg tccaacgcaa aaacaggggt atctttccca ccttggcatc aaggtcctgt
     4681 ttcatctgtt ccataacttc agagtagtcg ccgcttaccc tgaagaggat cgtcctggga
     4741 agcatgctcg ccttctccaa cttgtcacag agcccctcgt ttagcataaa ggccaggatc
     4801 tcaccggggc tggaatcggc caggaaaacc cttatctccc caagcgatct ccccttcatg
     4861 gttgcaaata tcagggtctg accctcgctt gaactatccc acgcatcctc gtactgtttc
     4921 tcgacaaggg ggaatttggc tgccaccgat tcaataaccc cctggtcctt cagcttaagg
     4981 aagatctctc ttgtgcctcc cctgaccagc gaaagcattc tccctcaccc ctttcgatca
     5041 gagaccggtt ctacgcctgc agcacctggc atcgtcaacg tagggaggat agggtaacac
     5101 ctccacggtg ccgccttcta aaaggataac gttgatcccg ttaactccgt tgagatagag
     5161 gctgccctca tgatcgagag gcacctccac gtcgtgcccg aaaaggttga aggccgcagg
     5221 gggcaggggg agctcctcca gggagtgggc aagggctacg tccagtccgc agaggctcaa
     5281 cctgatcccc tcctcgaata ccatcacgcc cggaaaggca gaacggacca cctcggggag
     5341 atcgtggttc cctgtaatga tggcgaccct gtcccccggg accgcgctca ttacctccgc
     5401 aaggaggcga agcctgttcc tgaagatcct ggagtacccc ggtctgaggg ccagcttcac
     5461 gtcgtcgacc aggtcccccg tgtggacaag gcagaggggc tcaaggaact ccaaaagcct
     5521 cctgagatct ccgtaaaaga ccgacggcgt gtccgaaaca tggaccaggc acttccctct
     5581 ggcctcctgc aggtgcaagg gaagcggcaa caccgtacgg aaacaggcca gaaaatcctt
     5641 ccaggctccc atgcgttcgc tgttccccta ggacctgagt acggacatgc tgtcaggggt
     5701 caccattggg gacttgtggc agtgcacgag cttccagtcg gggttcttct ttgtgccgaa
     5761 gttgttgaag accctggtct cattgtggct cttatgcact atcttccccg gaaagactgc
     5821 cctcaccatg agcgtgaagg tgaccactgc cgtcgccccg aagacctgga ccttcggctg
     5881 aagtatctcg tgctcgaaga gctgctcctc ctcctccagt gggtcggggc ttccccccac
     5941 caccattcgg tgaacgcgga gctcccgcag gtggaattcc aggccatcga tcctctgggg
     6001 agatatgtac cactcgaaaa aagtacagtc ctcggaagtg gtgttactgt aagctgccac
     6061 atcaccctcc cagatgctct tcaggttctt ctcaagaatg gccatgatct ccttctccag
     6121 ggccttgttt ggctttgcca tgacctttca ccagccttcg tcaggtaagt tgttgatgaa
     6181 cattataact gttactgaaa agagctgttt cggcagagaa gacgcaaaaa agggggaagg
     6241 ttaaaacatt tacagtagag tctgcatgtt cttgggaaaa agggatgtct cttacttttt
     6301 tcttgccagg acaaccccga tgacgaggcc aagaagaagg cccatgacgt cggctgcaag
     6361 gtcggcaacc tcacagc
//
LOCUS       DAOPKQ010000066         6374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_13027_length_6374_cov_5.270929, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000066 DAOPKQ010000000
VERSION     DAOPKQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13027_length_6374_cov_5.270929"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK146_03475"
     CDS             <1..378
                     /locus_tag="PK146_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91334.1"
                     /translation="KKMETAISFGTAIASALLGRKVNTYSASRVGTAVSKAARLQKES
                     GDVARARETAGAVRQEIADLDARLEEEINAITTVGSPETEIQEEFIRPKASGMNLILL
                     GLLWLPYSRDAQGGIAPLWGLQE"
     gene            414..1025
                     /locus_tag="PK146_03480"
     CDS             414..1025
                     /locus_tag="PK146_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91335.1"
                     /translation="MSRRTVTVFAVLVFLSGGVLGWVRFSPVTPLSEEENREIPGAVS
                     PEMDGLPKADERMWASPGGREEAPEKHDFRPATGEPFSRSDLEASQTRSCDIIPERSE
                     RAVFDPGTIHPAVGDVAEQAREMVEDLDRRTLENTKKVLEAIPFVEIIPEKAELRPSG
                     DGVKLTITVDPQNISFGGKARNAGTPAEETASGDEGLQEEQAQ"
     gene            1090..1470
                     /locus_tag="PK146_03485"
     CDS             1090..1470
                     /locus_tag="PK146_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPE91336.1"
                     /translation="MKRTVATPDAPAAIGPYSQAVWAGSMLFVSGQICIDPKTGDLVG
                     NTAEEQAEQVLKNLGAILKSQGLSFESVVKATVFAKDMNDFSAVNDVYARYFKVDPPA
                     RVFVEVSRLPRDVLVEIDAVAVKD"
     gene            1557..1757
                     /locus_tag="PK146_03490"
     CDS             1557..1757
                     /locus_tag="PK146_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPE91337.1"
                     /translation="MQGTVKWFNEAKGYGFITPDEGNDVFVHFSAILGEGFKTLAEGQ
                     RVEFEVVQGEKGPQAANVEKIS"
     gene            1944..2219
                     /locus_tag="PK146_03495"
     CDS             1944..2219
                     /locus_tag="PK146_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPE91338.1"
                     /translation="MNKSELVQAVAKAAGLSNSKATQVVSTVIGEIAGALAKGNKVQI
                     AGFGTFEVRQRAAREARNPQDPTKKVKVPARKVPAFRAGKALKDKVS"
     gene            2354..3739
                     /locus_tag="PK146_03500"
     CDS             2354..3739
                     /locus_tag="PK146_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE91339.1"
                     /translation="MTDKDQLYDSSPIRLEGISRADLFEHSGAANLIVDTDTTILAVN
                     VMFETVTGYSRSEVEGTLSWRDLVHPEDRERMEEYHRLRRVDPELVPANYGYRFITSV
                     GQVRWGLLTGATLADDSRTLLSVVDITELKEKESALGRAEELYRGIVQTQTELISRFT
                     PDFTLTFVNDAYCRYFGETREQLVGSSFVHHVLPEDLADQRAYFALLTPERPENEMEE
                     RAILSSGEVRWQRWHDRGIFDEKGNLIEIQSIGRDITDLYLTREKLDSTIEKLKNSFA
                     RAIEMAGKIIEVRDPFTAGHQRRVARIAKEMASTLGLSRNEVTAIELAALAHDVGKIH
                     VPSEILSKPGVLQPTEWSIIRQHPVYSAEILKTLVTPWELADIALQHHERYDGSGYPQ
                     GLSGQEIRIEARVISVADVIEAMSSHRPYRPGVGIEKALEEVEAGRGTLFDPAAADSA
                     LYIFRKKGFEI"
     gene            3736..4617
                     /locus_tag="PK146_03505"
     CDS             3736..4617
                     /locus_tag="PK146_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPE91340.1"
                     /translation="MKNLVRMKLEAQEVVTGPFVGINSPDMVEILGLSGFDLCVIDTE
                     HGPMDPESIQPLIRAAELSGMTPVVRVPRAEPVEILKVLDVGAHCIHVPQVNSPETAR
                     VVARAARYHPAGMRGVAVPRALSYGLRPLGEALEEANREVMVIVHCENVLGLDRIDEI
                     AAVEGVDMVFVGPYDLSQSLGVPGEIFHPSMVKAVSRALEAIKRAGKPAGIFVTSPEE
                     AERRIGEGFRYIGYSVDSLIFGKACREIVSTLKSRRQDTWCTPPDGPWRPRRRGTCPY
                     RRGTPSSPSPPCRGIPC"
     gene            complement(5033..6274)
                     /locus_tag="PK146_03510"
                     /pseudo
     CDS             complement(5033..6274)
                     /locus_tag="PK146_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
BASE COUNT         1382 a         1762 c         1916 g         1314 t
ORIGIN      
        1 aagaagatgg agacggccat atcctttgga acggccattg caagtgcttt gcttggaagg
       61 aaggtcaaca cctattctgc gtccagggtg gggacggctg tctccaaggc agcccggctg
      121 cagaaagaga gcggcgacgt tgccagggcc agggagaccg ccggggcggt ccggcaggag
      181 atagccgacc ttgatgccag gcttgaggaa gagatcaatg ccataaccac tgtgggatcc
      241 ccggagacgg agatccagga ggagttcatc aggcccaagg cttccgggat gaaccttatt
      301 cttctgggcc ttctctggtt accatactcc cgtgatgccc agggagggat cgcacccctt
      361 tggggtcttc aggagtgatc ggggtacctg gtctgcctgg taagtgagga cccatgagca
      421 gaaggactgt tacagttttt gccgtgctgg tttttctttc gggaggggtg ctgggctggg
      481 tgaggttcag cccggtaact cccctgtccg aagaagagaa ccgtgagatc ccgggagctg
      541 tatctccgga gatggatggg ctaccgaaag ccgatgaaag gatgtgggcc tccccgggag
      601 gtcgggaaga ggctcctgag aagcatgatt tccgtccggc cacgggagaa cccttcagca
      661 gaagtgacct cgaggcctcc caaacccgat cttgcgatat catccctgag agatccgaaa
      721 gagctgtatt cgatcccggg accatacacc cggccgtcgg ggatgtggcc gagcaggccc
      781 gggagatggt cgaggatctg gacaggagga ccctggagaa cacgaaaaag gtcctggaag
      841 ctatcccctt tgttgagatc atccccgaaa aggcagagtt gcgcccctcc ggtgacgggg
      901 tgaaactgac aataacagtg gatccccaga acatctcttt cgggggaaag gcccgaaatg
      961 ccggaacccc cgccgaagaa acagcatcgg gggacgaggg actccaggaa gaacaagcgc
     1021 agtgatccat gttttacaga cccccgggag atagatcttc cgggacaaga caatggaggt
     1081 gttcaagtga tgaaaagaac cgtagccaca cccgatgcgc cggcggcgat aggcccttac
     1141 agccaggcgg tatgggccgg cagtatgctg ttcgtatcgg ggcagatctg tatcgacccg
     1201 aagaccggag atctggtcgg caataccgca gaggaacagg cagagcaggt gctgaagaac
     1261 ctcggggcca ttctcaagtc ccaggggctt tcctttgaga gcgtggtgaa agccactgta
     1321 ttcgcaaagg atatgaacga cttttcagct gtcaacgatg tctacgccag gtatttcaag
     1381 gtcgacccgc ccgccagggt ctttgtcgag gtctcccggc tgcccaggga tgttctggtt
     1441 gagatagatg ccgttgccgt aaaggactag agcattgaca aagaggagga aaatacgtac
     1501 tgttttatca gccggttaaa accggcagat tctgctgtat gggaggcatt atctaaatgc
     1561 agggtacggt caaatggttt aatgaggcaa aggggtacgg tttcatcacc cccgacgaag
     1621 gcaacgatgt ctttgtccac ttcagcgcga tcctgggaga aggattcaag acgcttgccg
     1681 aagggcagcg tgtcgagttc gaggtagtcc agggggagaa gggtccccag gcggcaaacg
     1741 tagagaagat atcctgacct tattccttaa gacgacgtat agcaggcatg acagactcaa
     1801 ggcggtaccc tcgatcgggt accgcccgtt cttttccttt taaccctgcc gagagtctca
     1861 aaaaccttcc agggcttgcc ctgtggcccc ggggatgcga taatcccttt gaacaaagcc
     1921 ttttcgagag gaggtcgaca gtagtgaaca aatcggaact ggtccaggcc gtggcaaaag
     1981 cagcaggtct gagcaacagc aaggccaccc aggtggtgtc gacagtgata ggcgagatcg
     2041 cgggagccct cgccaaggga aacaaggtcc agatagcggg ctttggtacc ttcgaggtca
     2101 gacagagagc agcaagagag gcacgtaatc cccaggaccc caccaagaag gtcaaggtcc
     2161 ctgcaagaaa agtccccgcc ttccgtgcag ggaaggcgct caaggacaag gtcagctagt
     2221 tcttttattt cttttccgcc ccgcaaaggg gcggtttttt tattccttcc ggtcaccttt
     2281 gtctcccctt taacctttac cctttttcat ttactattga gccgcaggca caattccatc
     2341 caggggtgtt cccatgaccg ataaggatca attgtatgac tcaagcccta tccggcttga
     2401 ggggatctca agggccgatc tcttcgagca ctccggggcc gccaacctta ttgttgacac
     2461 ggacacgaca atactggcgg taaacgtcat gttcgagaca gtaacgggct actccaggag
     2521 tgaggtggag gggaccctga gctggaggga tctcgtacac cccgaggaca gggagagaat
     2581 ggaggagtac catcgccttc gacgggtgga ccccgaactg gtccccgcca actacggata
     2641 ccgtttcatc acctccgtcg ggcaggttag atggggtctt ctgaccgggg ccaccctggc
     2701 cgacgactcg agaaccctct tgtccgtcgt ggatattacg gaacttaagg agaaggagtc
     2761 ggccctgggg agggccgagg agctctatcg ggggatagtc cagacccaga cggagttgat
     2821 atcccgattc actccggact tcacgctgac cttcgtcaac gatgcctact gccgctactt
     2881 cggggagacc agggagcagc tggtagggag ctccttcgtt catcacgtcc tccccgagga
     2941 ccttgccgac cagagagcct actttgcctt gctgacccct gaaagacctg aaaatgaaat
     3001 ggaagagaga gccattcttt cttcaggaga ggttcgctgg cagcgctggc atgaccgggg
     3061 catcttcgac gaaaagggca atctcatcga gatccagtcc atcggaaggg atattacgga
     3121 tctctatctg acaagggaga agctcgacag caccatcgaa aagctcaaga actcctttgc
     3181 ccgcgccatt gagatggcgg ggaagatcat agaggtcagg gaccccttca cagcaggtca
     3241 ccagagacgg gtggccagga tcgcaaagga gatggcctcg acgctgggcc tgtcgaggaa
     3301 cgaggtaacc gccatcgaac ttgcggccct tgcccacgac gtgggcaaga tccatgtccc
     3361 cagcgagatc ctgagcaagc cgggagtgct tcagccgacg gagtggtcca tcatcaggca
     3421 gcaccccgta tacagcgccg agatactgaa gaccctggtg accccctggg agctggccga
     3481 catcgcgctg cagcaccatg aaaggtatga cgggagcgga tatccccagg gactgtccgg
     3541 ccaggagata aggatagagg ccagggtgat ctccgttgcc gatgtgatcg aggccatgtc
     3601 ttctcaccga ccataccgcc cgggggtggg gatcgaaaaa gccctggagg aggtcgaggc
     3661 gggaagagga accctctttg atcccgccgc cgccgattca gctctctata ttttcaggaa
     3721 gaaggggttc gagatatgaa aaaccttgtc aggatgaaac tggaggccca ggaggtggta
     3781 acaggcccct ttgtggggat caacagcccg gacatggtgg agatcctggg tctgtcaggc
     3841 ttcgatctct gcgtgatcga cactgaacac ggtcccatgg atcctgagtc gatacagccg
     3901 ctgataaggg cggccgagct ctcggggatg acccccgtgg tccgtgtgcc ccgtgccgag
     3961 cccgtggaga tacttaaggt cctcgacgtg ggtgcccact gtatccacgt cccccaggtc
     4021 aactcgcccg agacggcccg ggtggtagca agggccgcaa ggtatcatcc ggccgggatg
     4081 cggggcgtgg cagtgccgag agcgctatcc tacgggctcc gtccccttgg tgaagcgctg
     4141 gaggaggcca accgggaggt aatggtgata gtccactgcg agaatgtctt ggggcttgat
     4201 aggatcgacg agatcgccgc cgtggagggg gtagacatgg tctttgtcgg tccctacgac
     4261 ctttcccaat ccctgggggt acccggggag atcttccacc cttccatggt gaaggccgtt
     4321 tcgcgggctc tcgaggccat aaagcgcgca ggaaagcccg cgggtatctt cgtcacatct
     4381 cccgaggagg cagaacgccg tatcggggag gggttccgct acatcggcta cagcgttgac
     4441 agccttatct ttggcaaggc ctgcagagag atcgtcagca ccttgaaaag ccgcaggcag
     4501 gacacttggt gcacccctcc tgacgggcca tggaggcccc gcagacgggg cacatgtccg
     4561 tatcggagag ggactccatc atccccttct cctccatgtc gaggtatccc atgctgagca
     4621 gcccgtaggc tatcgcgtcg ggaaccgaca tgatcacacc gtcgtcgtgc catacgggct
     4681 gggatccccc gatgcccttc atctgcttga ttatggcgtg gatcctgacg ccgctcctca
     4741 gggagagaga tatgagcctg cccattgcct cggtgaatgc cgttgtctcg tgcccagatt
     4801 tgcccatagt ggtgaatacc tccacggggc gtccgttcac ctcgttcacg gtgaggtaga
     4861 ggttgccgta gcttgtgggg atcttggcgg tcttgccgcc caggatgaag gggcgctcca
     4921 tggggctctc cacttccgat atctgcggga tcgggagggc ctgctggacg gcaggcctgt
     4981 caacatagag gacctggccc gaacggcagc cgtcgcggaa gacggtgatc cccttgcagt
     5041 gggtcttcca ggctaggacg taggctttct cgacgtcctc cacagtggcg ctgttgggga
     5101 gattgatggt cttgctcacg gcgttgtcca cgtactcctg gaaggccgac tgcatcttta
     5161 catgccactc gggggctatc tggtgagacg tcttgaagac cctggcgatc acgtcctggg
     5221 actcgcccat ctcaagtgcc tccacgtagt aacggttccg gtaggagaac tgctttccct
     5281 ccaggacctc cttggtgaac tccagcgcaa agaccggctc tatccccgat gagcagtcgg
     5341 cgatcatcga cagggagcca gttggagcta tggtggttat ggtggagttg cgcaggcccg
     5401 aatcgggaag tgcggggcat acccccctgg tcagcgccag ggcggcgctc tcctgtcggc
     5461 ctacggtgtt aatggccgat atgacgctcc ttcccagctc gagggcctca tcagagtcgt
     5521 agggtatcct cagctggaag agcatgtcgg cccaccccat cactcccagg ccgatcttgc
     5581 gggtcttctt gaccatctcc tcgatctggg gcagggggta acggttcacg tcgatcacgt
     5641 cgtcaaggaa ccggacggcc agcctcgtgg tgagcctcaa tttctcccag tcgaccccgt
     5701 tgtccttgac catgtgaccc aggttgatgc tccccaggtt acacgcctcg tagggcagca
     5761 gcggctgttc gccacagtta tggactacaa gcccgttggc atcaaaggca ttctcgccgg
     5821 gaacaattac gtcaaagact tcctcaatgc actcttggac cagcaggtct acagttgcaa
     5881 taaaatactc cctgttcata ttccgcctgt agtgttcaag gcttcccatg agttttccct
     5941 gtttgtcgga gtccctgaaa cctaccttct ccgaaaaaag cctgatattt tccctggaga
     6001 tcaccagttc atgctgagca agtgtctggt aggctttcat gccccccttg ccgtcaggca
     6061 gaagggccga ccgttctttg cgccggtttt tgtatatagt gcttgctatg ccaaacttca
     6121 aaagcatcct ttggacagcc tgaagggttg gcaaagaact ctggctaagc cggacgctta
     6181 ttcccttttc ctgttctccc tggactgaac cgtcagcatc gaagagtcca ctgaggaaac
     6241 cctttacaaa agcggaagag gtatcggtct ccattttccg cgtaatccct ttgtttccgg
     6301 aggacatgcc aaggtatgat gcgagcgatc cgatcgcgct tgtggacatt ctgtattcgt
     6361 tcctgcccct tacc
//
LOCUS       DAOPKQ010000067         6312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_13223_length_6312_cov_4.054499, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000067 DAOPKQ010000000
VERSION     DAOPKQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13223_length_6312_cov_4.054499"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="PK146_03515"
     CDS             complement(<1..1585)
                     /locus_tag="PK146_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPE91341.1"
                     /translation="MNLIGNSVRRPVLTTVIMTILVLLGLYSFYQLGVALLPKMDIPV
                     VLVRVAYRGAGPLEIERLIVQPVEDAIASVEGVKEINGYALEGTGFVVAVLEYEVDVT
                     QATLDISTRVKAITSDLPDDADEPVVDKIDINAQPFMMLVAKTGLPSYQARDIVEDQV
                     AKRLTQIIGMASVDISGGRVREIHLDADPSSLMAHNLSLRRLASLVKASNFSSPSGNI
                     TLGGRETSVRIVGEPSQSSQFGDIGIPMGEGRVVRLGNVVNVVDGLADERNRARFDGK
                     DSILLELIARPNANIVRVSKAVRTELERIAPSLGNSVELEVVYDSSEFVEKAVKNVIR
                     DMVIGTLLTAFFIYLFLRQFGSTLAVAIAMPTSIIATFIPMFFFQYTLNVMSTLGLAI
                     SVGVLVNNAILVLENIYRYRELGMDPITAAEEGTREISLAVLSTTATNLGVFIPIAFM
                     GGIVGQFFNQFALTVVFSTLFSLWGAFTVTPMAAARLGGSIHVSSFAKKATAWWHWLY
                     REVEVFHHIYVEKAVRHPWL"
     gene            complement(1585..2664)
                     /locus_tag="PK146_03520"
     CDS             complement(1585..2664)
                     /locus_tag="PK146_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPE91342.1"
                     /translation="MNSMKKGKTSYRWIWWLLLILLVAFGAWRIFFRPVPQPPVSVAE
                     IRSREGIPVTVHEVASSHWEHWINFFGRAQASSEVTIAAERQEYVSSVNVDVGDQVRN
                     GQVLATLDVRVTRERLAAQEASVAEKQGRYRRLSALRSAGGASVQEVEAALSDAKSAQ
                     ASLKDIQMSLSRMTITSPLDGIVTGRSVETGNLVSPGQTLFSVADLEAIEVVLDVSPK
                     DIFKIVRGMPARVRTPNGWIKAGIKRVDPVADSSTGLFSVILSVPPGSGLSPGQALEA
                     QVQDEDIADAIVVPYEALKQIGGERTVVYVETDGVAEERDVITGGSYEGSVRILSGVE
                     PGQKVVVKGSDRMFPNARIWVQEDQ"
     gene            complement(2666..3952)
                     /locus_tag="PK146_03525"
     CDS             complement(2666..3952)
                     /locus_tag="PK146_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPE91343.1"
                     /translation="MISFFRESHATKVLIAFGLVLFSLSIPGRAGAVGMTWEEAWNII
                     LQNNPGIKAAKETVSAAHASLKLTAVPTKVTAGLSGTTTKNEGINSSSSAGLTLSYNT
                     SLFGREKEAIRAETAGLREAETALDAAVLRLYHNAAMAFWGAAAGEASVRAAEDEIVK
                     REAFLQDARLRYEQGMVPELDVMRAESALAEARHSLALQQASRSGFEAMLKGLAGWRD
                     IRPAEGLFEVDLPVEPERDRPDFESVARDHPSVVRARWGMERAEHMLRVAEMISLPTL
                     GMSATMTLLTDGTASQQYSQDEWWGRATLTIPVIDGGQARWTTERARAALASAEASVG
                     SATAEVMMNLLSAWEDYTAAIKGFEAERSRFSTVSREREIVLLRYREGLANQIEVLDA
                     QTRFASSVASLINARRGLLVAEAALASAEGKLPMEE"
     gene            complement(4037..5662)
                     /locus_tag="PK146_03530"
     CDS             complement(4037..5662)
                     /locus_tag="PK146_03530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE91344.1"
                     /translation="MRLRILGAAGEVTGSNYILETGTSRLMIDCGIFQGGNEDRNSEP
                     LDFDPSTIDAVILTHAHLDHTGRVPLLVKMGFNGKVIATTPTVELTDVLWRDSAKIQK
                     EDAEWKSRKNKRKGLPPEEPLFSDEDVDKATAMLVPVAYDDIIEPAPGFRVRFRDAGH
                     ILGSANLEIWGDSEGREVKVVFSGDLGPQKTVMERTPSVIEDADYVVIESTYGDREHR
                     TNEESRQEFREAMKIMIQDHGKVLIPTFVVDRAQRVLYELMLMQKEGILPSSVPIFFD
                     SPMGLKATEVYNKHLSLLSSEIQEQIRAGNDPFSPDNLRIVSDVEESKNINEIGEAVV
                     LAGSGMCNGGRIVHHLKNNLFMETTDVVFVGYQARGTLGRRIVEGEKVVRVAGEEVAV
                     KAKIHTINGFSAHADRRDLLAWATQFRTDPVFFITHGELESSQALAKTFEENGIRSVV
                     PEAGREYDLTGEEEKDLTVAKAPERVAVSPGVPCRGLGAVEAVLCEIVSIATELREDG
                     STARGDEALNLAVSARTLLRSLKREKEVKKVSE"
     gene            5788..6303
                     /locus_tag="PK146_03535"
     CDS             5788..6303
                     /locus_tag="PK146_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HPE91345.1"
                     /translation="MDPLGAILILGARLLDVSLGTFRILLLVRGKKLHASMTAFFESA
                     IYLVALGYVLQQGVRGWLQIAAYAGGYALGNFLGATLEEKILSSFVLVEVVGPVTDEM
                     LETVENLREEGYGTTVVTGSGKTGERIILKIICQRNDIGHVAQMIGNRGFVFTSEVKA
                     VWGGYFRQKRK"
BASE COUNT         1279 a         1900 c         1706 g         1427 t
ORIGIN      
        1 tcagccatgg atgccttacg gccttctcta catagatgtg gtgaaaaacc tcgacctcgc
       61 ggtaaagcca atgccaccag gcggttgcct tcttggcgaa ggatgagaca tgtatggagc
      121 cgccgagtct tgcggcggcc attggtgtaa ctgtgaacgc accccagagg gagaacagtg
      181 tcgagaatac caccgtaagg gcgaactggt tgaagaactg acccacgatc ccccccatga
      241 aggcaatggg gatgaagaca cccaggttgg tggccgtcgt cgagaggact gccagggaga
      301 tctctcttgt accctcctcc gccgcggtaa tagggtccat gccaagctcc ctgtaacggt
      361 agatgttctc cagaacgaga atggcgttgt ttaccaggac ccccaccgag atggcaagcc
      421 ccagtgtgct cattacgtta agcgtgtact ggaagaagaa catggggata aaggtggcaa
      481 ttatggatgt cggcatcgct attgccacgg ccagggtcga accgaactgc ctgaggaaca
      541 ggtatatgaa gaaggctgtc aggagggtcc ctatgaccat gtccctaatg acgttcttga
      601 cggctttctc tacgaactcg ctggaatcgt ataccacttc gagttcgacg ctgttcccga
      661 gtgagggggc tatcctctcc agttcagtcc tgacagcctt tgagaccctt acgatatttg
      721 cgttgggcct tgctatcagt tccagaagaa tggagtcctt cccgtcgaac ctggcacggt
      781 tcctctcatc cgccaggccg tcaacgacgt tgacaacgtt ccccagcctg actacccggc
      841 cctcacccat ggggatcccg atatcaccga actggcttga ctgggaaggc tcgcccacga
      901 tacggaccga agtctccctc cctcccaggg taatgttccc cgaaggtgag ctgaaattgg
      961 aagccttgac aagggatgca agcctccgaa ggctcaggtt gtgggccatc aggctggaag
     1021 ggtcggcgtc gagatggatc tcccttactc ttcctccgct tatgtccacg ctggccatcc
     1081 cgattatctg ggtcagcctt tttgccacct ggtcctccac tatatccctg gcctggtatg
     1141 agggaagtcc tgtcttggcg acaagcatca tgaagggctg ggcattaatg tcgatcttgt
     1201 caaccaccgg ttcatcggca tcgtcgggca gatctgaggt gatcgccttc actctggtcg
     1261 atatgtcaag cgtggcctgg gtgacgtcga cctcgtactc aagaacggcc acaacgaagc
     1321 cggtgccctc cagggcgtaa ccgttaatct ccttgacacc ctccacggag gcaatggcgt
     1381 cttctacggg ctggactatc agcctctcga tctccagggg gcctgctccc ctgtaggcga
     1441 cccgaacaag gaccaccgga atgtccatct tcgggagaag ggccactcca agttgataga
     1501 aagagtaaag tcccagaagg accaggatcg tcataataac cgtcgtcagg accggtcttc
     1561 tgacggagtt gccgataagg ttcattactg atcctcctgg acccagatcc tggcattggg
     1621 gaacatcctg tctgaaccct ttactactac cttctgacct ggttccactc ctgacaggat
     1681 ccggaccgac ccttcgtagg atcccccggt gataacatcc ctctcctccg ccactccatc
     1741 cgtctctaca tagacgacgg tcctttcgcc gcctatctgc ttgagtgcct cgtaggggac
     1801 gacgatggca tcggcaatgt cctcatcctg aacctgggcc tccagcgcct gtcccgggct
     1861 cagcccggaa ccgggaggga ccgaaaggat cactgaaaaa aggccagttg agctgtccgc
     1921 aacaggatct accctcttga tccctgcctt gatccatcca tttggagtcc tcaccctcgc
     1981 aggcatgccc cttactatct tgaatatgtc cttgggggag acgtcgagca ccacttcgat
     2041 ggcttcaagg tccgccacgg agaagagcgt ctgtcccggt gatacaaggt tgccagtctc
     2101 cactgaacgg ccggtaacga ttccatccag gggtgacgtg atggtcattc tcgaaaggga
     2161 catctggata tccttgaggg aggcctgggc tgacttggca tcagagagag cggcctcaac
     2221 ttcctggacg ctcgcccctc ctgctgaccg aagggccgaa agacgcctgt acctcccctg
     2281 tttctcggcc acagaggctt cctgtgcggc cagcctctcc cttgtgacac ggacgtccag
     2341 ggtggcaaga acctgcccat tcctgacttg gtcaccaacg tcaacgttga cagaagagac
     2401 gtactcctgg cgttctgctg ctattgttac ttctgaggac gcctgggccc ttccgaagaa
     2461 gtttatccag tgctcccagt gcgaggaggc tacctcgtgt acggtcacgg ggataccctc
     2521 ccgggaccgg atctcagcaa cgcttacagg gggttgaggt acaggcctga agaagatcct
     2581 ccaggccccg aaggcgacca gcaatattaa aaggagccac cagatccatc tgtaggaagt
     2641 ctttcccttc ttcatactgt tcatgctatt cctccatcgg taacttgcct tcggcagaag
     2701 ccagggctgc ctcagccaca agaagccccc gtcttgcgtt gataagcgag gccactgaac
     2761 tcgcaaaacg ggtctgtgcg tccagtacct ctatctggtt ggccagcccc tccctgtatc
     2821 ggaggaggac gatctctctc tcccttgaga ccgtgctgaa cctggacctc tcggcttcga
     2881 aacccttgat ggctgcggtg taatcctccc aggccgacag gaggttcatc attacttcag
     2941 cagtggcaga ccccacggag gcctcggcag aggccagtgc tgccctggcc ctctccgttg
     3001 tccaccgggc ttgccctccg tcgataacgg ggatcgtgag agtggccctc ccccaccatt
     3061 catcctggct atactgctgt gaggccgtcc cgtcggtcag gagggtcatc gtcgccgaca
     3121 ttccaagggt gggcagtgag atcatctcgg caaccctcag catgtgctcc gctctctcca
     3181 ttccccaccg tgcccttaca actgaggggt gatccctggc gacagattcg aagtcaggcc
     3241 ggtctcgttc tggctcaacg gggagatcca cctcgaaaag gccttccgcc gggcggatat
     3301 ccctccatcc cgcaagacct ttcaacatag cctcaaaccc tgacctggaa gcctgttgga
     3361 gggccagaga gtgcctggct tcagcgaggg cgctttcggc ccgcatgacg tcgagctccg
     3421 ggaccatgcc ctgttcgtac cttagcctgg catcctgaag aaaggcctcc ctcttgacga
     3481 tctcatcctc ggcggccctg actgaggcct ccccggcggc agctccccag aatgccatgg
     3541 cagcgttatg gtagagcctc agaacagcag catcaagggc ggtctccgcc tctctcagtc
     3601 ctgccgtctc ggcccggatg gcctctttct cccgcccgaa gaggctggtg ttgtaagaaa
     3661 gggtgagccc cgcgctggaa gatgaattga tcccctcatt cttggtcgtg gttcccgaga
     3721 gcccagcggt gacctttgtc ggcaccgcgg taagcttcag ggaagcgtgg gccgctgaaa
     3781 ctgtctcttt tgccgctttg atcccgggat tgttctgcag gatgatgttc caggcctctt
     3841 cccaggtcat ccccactgca ccggcccttc ccggaatgga cagggagaac aggaccaggc
     3901 cgaaagcgat cagtaccttt gtagcgtgac tttctctaaa gaatgagatc attatcgtta
     3961 tcaccaccag tctttcaaaa aagggtccac agaattctag cacagggggg gggctgctgt
     4021 tccatgggaa cagggctcac tcgctgacct ttttaacctc tttctctctc ttcaacgacc
     4081 tcaggagcgt tctcgcggag acggcgaggt tcagggcctc gtctccccgg gcggttgaac
     4141 cgtcctctct cagctccgtg gcaatggaga cgatctcgca gagaacggcc tccactgccc
     4201 caagccccct gcaggggaca ccggggctca cggcgaccct ctcgggcgcc ttcgccacgg
     4261 tcaggtcttt ttcctcttcc cctgtcagat cgtactccct cccggcttct ggcacgacgg
     4321 accttatccc gttctcctcg aaagtcttgg ccagggcctg ggaagactcg agctctccat
     4381 gagtaatgaa gaacaccgga tcggtcctga actgcgtggc ccaggcaaga agatcccggc
     4441 ggtctgcatg ggccgagaag ccgttgatgg tatggatctt ggcctttacg gccacttcct
     4501 ctcctgcaac acggaccacc ttctctccct ccacaatgcg cctcccaagg gtacctctgg
     4561 cctggtagcc tacgaaaaca acgtcggtcg tctccatgaa caggttgttc ttgagatggt
     4621 gaacgatcct gcctccgttg cacattccgc ttccggcgag gacaaccgct tctcctattt
     4681 cgttaatatt cttggattct tccacgtcgg agacgatcct gaggttatcg ggggagaagg
     4741 gatcgttgcc cgcccttatc tgctcctgta tctccgacga aagcagtgag aggtgcttgt
     4801 tgtagacctc tgttgctttc aggcccatgg gagagtcgaa gaatatggga acgctggagg
     4861 gaaggatacc ttccttctgc atgagcatca gttcgtagag gaccctctgt gccctgtcga
     4921 cgacgaatgt agggataagg accttgccat gatcctggat cattatcttc atggcttccc
     4981 tgaactcttg acggctctcc tcgttggtcc tgtgctccct gtccccgtag gtagactcga
     5041 taaccacgta atctgcatct tcaatgaccg aaggggttct ctccatgacc gtcttctggg
     5101 gtcccaggtc acccgagaag acgaccttta cctcgcgacc ctctgagtcg ccccatatct
     5161 cgagattagc gctgccaagg atatgccctg catcccggaa acgtaccctg aagcccgggg
     5221 caggctcaat gatatcatcg tatgccactg gaacaagcat ggctgtagcc ttatcgacat
     5281 cttcatcgga gaagaggggc tcttcaggtg gtagcccctt tcgcttgttc ttgcgggact
     5341 tccattcggc atcctctttc tggatcttgg cggagtccct ccagagaaca tccgtcagct
     5401 cgacggtggg cgttgtggcg atgaccttac cgttgaaccc catcttcaca agcagaggca
     5461 ccctgccagt gtgatccaga tgggcatgtg tcagtatgac cgcgtcgatc gtggagggat
     5521 cgaagtccag gggctcactg ttcctgtctt cgttgcctcc ctgaaagata ccgcagtcga
     5581 tcataagcct ggaggtccct gtctccagga tatagttgga tcctgtaacc tctccagctg
     5641 ctcccagtat gcgaagtctc atcggtttgc ccctgggggc tccctccttt cgaatatcct
     5701 ttcaattatt cccattctat actagaatcg catgacgggg tcgtacggcc tgtcacgatc
     5761 accataatcc accgggaggt aaccccaatg gatcctttgg gcgccatcct catccttgga
     5821 gcacgacttc ttgacgtttc acttggcaca ttcaggatcc ttctcctcgt aagggggaag
     5881 aaactccatg cttccatgac ggctttcttc gaatcggcta tctaccttgt agccctgggg
     5941 tatgtccttc aacagggggt caggggatgg ctgcagatag cagcttacgc cggaggctat
     6001 gcgctgggca acttcctcgg ggctaccctg gaggaaaaga tcctcagctc cttcgtactt
     6061 gtcgaggtgg tcgggcccgt caccgatgaa atgctcgaga ccgtcgagaa cctcagggaa
     6121 gagggttacg gaacgaccgt cgtgacaggt tcagggaaaa cgggcgagag gatcatcctc
     6181 aagatcatct gtcagcggaa cgacataggc cacgttgcgc agatgatcgg caacaggggc
     6241 tttgttttca cctccgaggt aaaagcggta tggggcgggt acttcaggca aaagaggaag
     6301 tgagcgacca gc
//
LOCUS       DAOPKQ010000068         6091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_13898_length_6091_cov_5.320411, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000068 DAOPKQ010000000
VERSION     DAOPKQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13898_length_6091_cov_5.320411"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PK146_03540"
     CDS             complement(<1..941)
                     /locus_tag="PK146_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91346.1"
                     /translation="MFILLTGNSAAGPSELCRFSPRAEADFIWWGITTPAMRSSFCRG
                     PLTHPVLISRAARFLADFPTLVVRCGSIDAPVCPYMETGARPGGDPAAGPAVPGAKLL
                     WDSGFSLGRLLAGLCERLVIGESCTGNGVTSRLTLRALGYRADVLEDFSSGKEGSSPW
                     ERASSRLGIRPGDMIGHGFEAATELGDPVLVTATAITAGAMGGPDILLAGGLPMLAVS
                     AILRDMGEKGRISISTTTRTEEDLGGAFGTISELLDLQVQRAEIGSVSDGAGASGAVL
                     LSGKYDITPERVLQRVSYLCDEISDQGRPGSPPAQGDR"
     gene            complement(926..1522)
                     /locus_tag="PK146_03545"
     CDS             complement(926..1522)
                     /locus_tag="PK146_03545"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPE91347.1"
                     /translation="MTTAYFDGASRGNPGESAAGACIVSEDGKVIWEFSRALGIGTNN
                     EAEYKALIMLLEEIASRGLKKITVRGDSRLVVSQVTGKWKVREERLRPLAERAKDLVR
                     ATGAALEWVPREKNSMADALSNKAFLSPAQGMASFPPENLERVCDTIFIAHGSADYAV
                     DVRHRSCSCPSFRNRGDCKHLQAALSLMKKGDGNCSSC"
     gene            complement(1812..1988)
                     /locus_tag="PK146_03550"
     CDS             complement(1812..1988)
                     /locus_tag="PK146_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91348.1"
                     /translation="MWHKDSAGNWYMSETKTIEAEDAAPAKESWQGKTLSTAFPGMGP
                     DSSIFLFSSKVAGQ"
     gene            2698..4491
                     /locus_tag="PK146_03555"
     CDS             2698..4491
                     /locus_tag="PK146_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPE91349.1"
                     /translation="MYGKFLHVDLGSGSFRDHVVPEEIARKYIGGKGLGVYLLDSMMA
                     PGTGALDPGNVLIFLTGPFTGTAFPTSGRFVVVTRSPLTGMYVDSHAGGHFAKELRMA
                     GYDGIIVTGKADRPAYLWLEDGKAELRDASDLWGKTVDDTVFAVREKTDGNARVAVIG
                     PAGENLVPIATITMDSDSDKSRAGVAGRGGTGAVMGSKNLKAIAVRGTGSIPIAEEGP
                     FREQVAEIVKAINANDQIHTRRIVGTSSLVESMSRTGILPSYNFQQGYFVPIYGLVST
                     NLRHYTKKDVACSNCPIICGKVLEDDGHPVKVEYESIALLGSNNGVGKMPLLTKAVRI
                     CNQLGLDTISAGGIAGFAMECRQRGILPEAPEFGDAEGQAKLLEDVAYRRGLGEVLAN
                     GVKAAAAEIGNDTDHFAIHVKGLELPGYEPRATWGMALAYATSDRGGCHQRAWTVLGE
                     LDGHLPRFSTEGMAKTVKTVQDERAAAYSLVVCDFAPYAQERALACLRHVTGWDISEE
                     EYLTAGERIWNHIRMFNIREAGISRKDDTLPPRMFEDPLPMPPRGEEKVSLKREDFDR
                     MLEEYYELRGWDSDGRPKAETVERLGIQVRI"
     gene            4511..5542
                     /locus_tag="PK146_03560"
     CDS             4511..5542
                     /locus_tag="PK146_03560"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPE91350.1"
                     /translation="MWNLGLVGFGNVGQGLARILEEKRCTLRERYGFECKVTFIADPV
                     KGSVLNSAGVDLRSILEEIDKNGTLKNHPDATEKDTLSLIRGGYAQVITEATYTDLTT
                     GEPGVSHIRTALEKGVHVVSTNKGPVSIALPELLALAESTGVSYFFEGVVMSGTPTLN
                     LAREALAGCDITGAMGIVNGTTNYILSRMEEGMDYDEALKKAQELGYAEADPTGDVEG
                     WDAAVKVQVLAKVIMGEPIKVEDVDRKGITDITPDDIASAKGRGARIKLIAEVGRDGG
                     KLVAKVAPGEVPLTHPLAGIMDATNALTLVTDHLGEVTIVGPGAGRVETGQALLTDLL
                     AVNRIHGKD"
     gene            complement(5539..>6091)
                     /locus_tag="PK146_03565"
     CDS             complement(5539..>6091)
                     /locus_tag="PK146_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91351.1"
                     /translation="GIEFILRATGGDPQQRRNAMYRLLSRRVDGFVWLDGEPEPDAGI
                     PSITVGRTRAGGASVSVDIGSAASQLTRLAEGRKIEVFQGENPLFPGIQVLLPGDTER
                     GGGTVRIFDGLLPPRNGSSSEGIAVVCATRRCPVVPSGAWKLYWPAFELGTLSARLLL
                     NTIQAKGVRPGAVSVLPVLSGPF"
BASE COUNT         1355 a         1675 c         1918 g         1143 t
ORIGIN      
        1 cggtcgccct gggccggcgg agaaccgggg cgaccctggt ccgagatctc gtcgcagagg
       61 tatgagaccc tctgaaggac cctctcgggg gttatatcgt acttgcccga gagcaggacg
      121 gcgcccgaag cgcctgcacc atctgaaaca ctcccgattt cagcccgctg gacctggagg
      181 tcgagcagct ccgaaatagt cccgaaggca ccgccaaggt cctcttcggt tcgggtcgta
      241 gttgatatgc tgatcctccc cttctctccc atgtcccgaa ggatggccga tacggcaagc
      301 atggggagcc ctcctgcaag gaggatatca ggaccaccca tggctcctgc ggtgatggcc
      361 gttgcggtca ccaggacagg atcgccaagc tcggtggccg cctcgaagcc gtgtcctatc
      421 atgtctccgg gtcggatccc cagtctggaa gaagcccttt cccacggaga agacccttct
      481 ttaccggagg agaagtcttc caggacgtcg gccctgtaac caagggccct gagggtgagc
      541 ctggaggtga ccccgttacc ggtacaggat tcccctatta ccaggcgctc gcaaaggccg
      601 gcaagaagtc ttcccaggct gaaaccgctg tcccagagaa gtttggcgcc gggaacggct
      661 ggtcccgccg ccgggtcccc gcctggtctg gcaccggtct ccatataggg gcataccggg
      721 gcatcgatgg agccacagcg gacgaccagg gtagggaagt cggccaggaa gcgggccgcc
      781 ctggagatca ggacggggtg tgtgagggga cctcggcaaa aacttgagcg catcgcgggg
      841 gtggtgatcc cccaccagat gaagtcggcc tcggccctgg gggagaagcg acagagttct
      901 gaggggccgg ctgctgagtt ccccgtcagc aggatgaaca atttccatcc ccctttttca
      961 tgagcgagag ggctgcctgg aggtgtttgc aatcgcccct gttgcggaag gaagggcagc
     1021 tgcaggagcg gtgccttaca tctacggcgt agtcggcgct cccgtgggcg atgaagatcg
     1081 tatcacagac acgttcaagg ttctccggag ggaaagaagc catcccctgg gccggcgaga
     1141 ggaaggcctt gttggagagg gcgtcggcca tggagttctt ctccctgggg acccattcaa
     1201 gggccgctcc tgtggcacgg actagatcct tcgccctttc cgccagagga cggagacgct
     1261 cctcgcggac cttccacttg cccgtcacct gggagaccac cagacggctg tcgccgcgaa
     1321 cagtgatctt tttgagacct ctggatgcga tctcctcaag gagcattatc agggctttat
     1381 actcggcttc gttgttggtg ccaatgccca gagccctgga gaactcccag atcaccttgc
     1441 cgtcttcgct gacgatgcag gctccggctg ccgattcgcc cggatttccc ctggaggcac
     1501 cgtcaaaata ggctgttgtc atggtttgca tatcccctcg catgaagtct tctgggaaaa
     1561 gtatagcaga ggggcagtat caagggcgtg agaagtgaga ggtgagaagt gaagacgtaa
     1621 atcgtgaatc gttaatcgta aatcgcaaga tcagcggcgt aaacagtgaa cagtgaacgg
     1681 tcaacgaaaa gggcaggatc agaagcagta gacatgagac gaagaatcca gaaccagggc
     1741 tggtgcaaca aaaaggcccg gaggataagc ctccgggcgc agaaccaacc gttcactgtt
     1801 tacgcagtta tctactgccc agccaccttg ctggagaaga ggaagatgct gctgtcgggg
     1861 cccattcccg ggaatgccgt ggaaagggtc tttccctgcc acgactcctt tgctggggca
     1921 gcgtcctctg cctcgatggt tttcgtttcc gacatatacc agttgcctgc gctgtccttg
     1981 tgccacatat caagccctcc gatcaggtct gctgaaagtt ttgtcgttgc tttgttatct
     2041 cctgaccgat ggctgcatct tattcgaagg accatgtggc gatgaacccc ctgtaaaaga
     2101 taagtttgaa gtctgaagcg ttaaaaactt ttgatcaaaa caaaaaagca acaaccgctg
     2161 ttgcagatcc cggctggatc gttaaaacac cgggcaacga tctggtactc acgatgtcaa
     2221 agtatatccg ctttctgagt tttgtcaact agagtttact tattttcata aaataaacca
     2281 actgggcctc agagtaacgg tcaagtcggg gctttttcac gatagaatat agcctggagg
     2341 ctatactatg atggtttttc aggaggtaaa aggtttgggt acgttcctga tctttttcat
     2401 gatcttcctc atgggtttcc tctggtagct ggggcttgcc tgtgacagca tcatccgctc
     2461 ccttggggaa tgttcaagga tcaaagaaga actgatatct ttctccggga agaccggggc
     2521 agcaggagag acctacaggc tcagcagaga cctgaaagag gccactcgag aggctaagag
     2581 ggagatccgc gacaccgttt cggagaggat catagcgaag ttattcggtt tttttagccc
     2641 cgcagaacag gcggaccaat acgccaattt ttccgatctc gcaagggggg aagagagatg
     2701 tacgggaagt ttctgcacgt cgatcttgga tcgggatctt tcagggacca tgtggtccct
     2761 gaagagatcg caaggaagta cataggagga aagggtctgg gagtctacct gctggattcc
     2821 atgatggcgc ccggaacggg ggcactggac cccgggaacg tcctgatctt tctgacgggg
     2881 cccttcacgg ggaccgcttt cccgacctcg gggcgcttcg tcgtggtaac caggtcgccg
     2941 cttaccggca tgtacgtcga cagtcatgcc ggcggacact tcgcaaagga actgaggatg
     3001 gcgggctacg acgggatcat cgtcaccggc aaggcggaca gacctgccta tctctggctg
     3061 gaagacggca aggcggagct ccgggacgcc tcggacctct ggggaaagac cgttgacgac
     3121 actgtctttg cagtccggga gaagaccgac ggtaacgccc gagtagcggt gataggtcct
     3181 gcgggggaga accttgttcc cattgccacc ataaccatgg acagcgacag cgacaagtcc
     3241 agagccggtg tcgctggacg gggcggcacc ggggcggtca tgggaagcaa gaacctcaag
     3301 gccatagccg tcaggggaac cggcagcatc ccaatcgccg aggagggccc cttcagggaa
     3361 caggtggcag agatagtcaa ggcaataaat gccaatgacc agatccacac tcgacgtata
     3421 gtggggacgt cgtcactggt ggaatccatg agccgaacgg gcattctgcc atcgtacaac
     3481 ttccagcagg gttacttcgt gcccatctac gggctggtct cgactaacct caggcactat
     3541 accaagaagg acgtggcctg ctccaactgc cccatcatct gcggcaaggt ccttgaggac
     3601 gacggacacc ctgtcaaggt ggagtacgag tccatcgccc tcctggggag caacaacggt
     3661 gttgggaaga tgcccctgct gacaaaggcc gtaaggatct gcaaccagct gggactggac
     3721 accatatcgg ccgggggcat agcgggtttc gccatggagt gccggcagag aggcatcctc
     3781 cccgaagcgc cggagttcgg cgatgccgaa ggacaggcta aacttcttga ggacgtcgcc
     3841 tacaggagag gtctcggaga ggtcctggcc aatggggtaa aggccgccgc cgcggagatc
     3901 ggcaatgaca cggaccactt cgccatccac gtgaaggggc ttgagcttcc cggttacgaa
     3961 cccagggcga cctggggaat ggccctggcc tacgccacct ccgaccgcgg aggctgccac
     4021 cagagagcct ggacggtgct cggcgaactg gacgggcacc tgccccgctt ctccacggag
     4081 ggaatggcaa agacggtcaa aacggtccag gacgagagag cagcagcata ttcgctggtg
     4141 gtatgtgact tcgctcctta cgcccaggag agggctttgg cctgtctcag gcacgtaacc
     4201 ggctgggata tcagcgagga ggaatacctc actgccggcg agaggatctg gaaccatatc
     4261 aggatgttca acatccgtga ggcagggatc tcaaggaagg acgataccct gccgccgagg
     4321 atgttcgagg acccgcttcc catgccaccc aggggtgaag agaaggtatc cctcaagcgt
     4381 gaggacttcg acaggatgct tgaggagtac tatgaactga gaggatggga cagcgacgga
     4441 cgccccaagg ccgaaacggt tgagagactg gggatccagg tgcgaatttg acaggaggtg
     4501 atctctggat atgtggaatc tgggtttggt aggattcggc aacgtgggtc agggactggc
     4561 aaggattctg gaggaaaagc ggtgtaccct cagggagcgc tacggtttcg agtgcaaggt
     4621 gaccttcatc gctgacccgg tgaagggttc ggttctgaac tccgccggcg tcgatctgag
     4681 gtccattctc gaggagatcg ataaaaacgg gaccctgaag aaccaccccg acgcgactga
     4741 gaaggacacc ctctcgctga tcaggggggg ttatgcccag gtgatcaccg aggcaaccta
     4801 cacggacctc accacaggag aaccgggcgt aagccatatc aggaccgccc tggagaaggg
     4861 cgttcacgtg gtatccacca acaagggccc cgtatcgatc gcactcccag agctgctggc
     4921 cctggccgag agcacggggg tctcctactt tttcgagggc gtggtaatga gcggaactcc
     4981 gacgctgaac cttgcccgcg aggccctggc cggctgcgac atcacgggag ccatgggtat
     5041 cgtcaacggg accaccaact acatcctttc ccggatggaa gagggcatgg actacgacga
     5101 ggccctgaaa aaggcccagg agctgggtta tgccgaagcg gaccccaccg gtgacgtgga
     5161 gggttgggac gcggcggtga aggtccaggt gcttgccaag gtcatcatgg gtgaaccgat
     5221 aaaagtggaa gatgtggatc gcaaggggat aacggatatc acgccggatg atattgcctc
     5281 tgccaagggc aggggtgcaa ggataaagct gatcgcagag gtcggaaggg atgggggcaa
     5341 gctggtcgcg aaagtcgctc caggggaggt ccccctgacc caccctctgg cggggatcat
     5401 ggatgccacc aatgcactta ccctggtcac cgaccacctt ggagaggtca ctattgtggg
     5461 acccggtgcc gggagggtgg agaccggcca ggcccttctg acagaccttc tggcagtgaa
     5521 caggatacac ggaaaggatt agaaggggcc cgaaaggaca gggagtaccg aaactgcgcc
     5581 ggggcggacg cccttggcct gaatggtgtt cagaagcagc ctggccgata gtgtgcccag
     5641 ttcgaaggcg ggccagtaga gcttccaggc cccggaaggg accacggggc atcgcctggt
     5701 ggcgcagaca acggcaatac cctctgaaga ggatccgttc cggggaggaa ggagaccgtc
     5761 aaagattctg acagtccctc ctcccctttc cgtatccccc ggcaggagaa cctgaatgcc
     5821 ggggaaaagg gggttctccc cctggaagac ctcgatcttg cggccttcag ccagccgggt
     5881 cagctgtgaa gcggcggaac cgatatcgac tgacactgag gcccctccgg ccctggttct
     5941 gcccacggta atggaaggga tgccggcgtc aggttccggt tcaccgtcaa gccagacgaa
     6001 tccgtcgacc ctcctcgaga ggagccggta catggcgttt cgcctctgct gcgggtcgcc
     6061 cccggtcgcg cggaggatga actcaatacc g
//
LOCUS       DAOPKQ010000069         6084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_13930_length_6084_cov_4.564936, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000069 DAOPKQ010000000
VERSION     DAOPKQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13930_length_6084_cov_4.564936"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PK146_03570"
     CDS             <1..1066
                     /locus_tag="PK146_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPE91352.1"
                     /translation="LPGEEEFPYAVRVVSDILESNGSSSMASVCGASLSLMDAGVPVR
                     KPVAGVAMGLIKEGEKVAILTDIQGLEDHYGDMDFKVTGTREGITALQMDNKAGGITR
                     SILERALDQARRGRLFILEKMESAITEPRDNVSAYAPRIYTLTVPLDKIRDIIGPGGK
                     MIRNIVSQTGVKVDVEDDGRVFVASPTTEAAEKALGMINDLIREVTAGEVFSGKVTRL
                     MAFGAFVEVLPGKEGLLHVSEISTTHVGKVDDVFSSGDSVLVMVKEIDDMGRINLSRR
                     RALERKNEPELAERFADAFVLEDAREERIKALPPSGDRDRNQRGGAPRPGGRPGGDRP
                     SGDRRSHSGPRRSSSGDRNR"
     gene            1069..1509
                     /gene="dut"
                     /locus_tag="PK146_03575"
     CDS             1069..1509
                     /gene="dut"
                     /locus_tag="PK146_03575"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPE91353.1"
                     /translation="MQEVVRLGIFRDDEDIALPVYATPFSSGVDLRSNERVTIPAGGR
                     SAVGTGIRLEIPEGYECQVRPRSGLALKNGVTVLNAPGTIDADYRGEVRVILVNLGEN
                     PFEIEKGDRIAQMILAPVSRIEWEETDSLSETDRGSGGFGSTGR"
     gene            complement(1756..3228)
                     /locus_tag="PK146_03580"
     CDS             complement(1756..3228)
                     /locus_tag="PK146_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPE91354.1"
                     /translation="MAEIKKADQWSTRWGLILSCIGMAVGTGNIWRFPRVAASQGGGA
                     FVIALLIGLFLWAIPLLMAEAVWGKVSRMGVIGSFKEMAGRKWTWMGTTVAVISLGIA
                     FYYSVVVGWCIRYFLYAITGVIKPGLDTEALWAAFSGTPGAMVPWHILTMVAATVILF
                     KGISQGLEKINKVLIPTLFVMLVVLAIRANMLPGSTEGLRYFFEPKWADLLKGRVWLE
                     AFTQVAWSTGAGWGLMLTYFVYTKKREDIALNATTVCFADTMAGFLAGLAIIPTIFAL
                     SPDPMAAVASGNTGLAFIHLTSLFTVMPGGVIFASIFFLALIFAGFSSLLSMVELGLR
                     LLMDAGWTRNKAAIAAGIGILVLGLPSAWSISFLDNQDWVWGVGLLLSGLIFSIAAMK
                     IGVNKIWDQYIAPDSDLNAPWMFKLIYLFPVWFVIIFWWWIAQAASWYPDTYMKWLPI
                     SEYTFTVGTMVYQWAIVVVLALVTNNWLADKSTVKFEATE"
     gene            3564..5486
                     /gene="thrS"
                     /locus_tag="PK146_03585"
     CDS             3564..5486
                     /gene="thrS"
                     /locus_tag="PK146_03585"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPE91355.1"
                     /translation="MPNYSGPEGLELNSSEPLKAREILSRWGLSEGSIAAVVDGTRID
                     LAAVVKSDSLVEPVLSSSPEGLEILRHSTSHLMAQAVQRLFPGTRLGIGPSIQDGFYY
                     DMEIAGQITEEDLPRIEEEMRKISSEDIPVERLLLPREEALDLFSERDAVYKVELVSE
                     IPDELISLYRQGDFVDLCRGPHVISTSQLKHFKLLSLAGAYWRGDEKNIMLTRIYGTA
                     FDTAEALDDHINRIEEAKRRDHRKLGRELDLFSIQEEGPGFPFFHPKGMVIINRLVDF
                     WRAEHSRRGYAEIRTPLILDQDLWIRSGHWDHYRENMYFTEIDERPFAVKPMNCPGGI
                     LVYKSRIRSYREFPMRIAELGTVHRHERSGVLHGLMRVRSFTQDDAHLYVTPEQIKQE
                     IIGIMDLVRFIYRDVFGFSYRVELSTRPEKAMGDPVMWEKAEKALQEALEDFGTDFRV
                     NPGDGAFYGPKIDFHLEDCIGRTWQCGTIQLDFQMPEKFDMTYIGPDGNEHRPVMLHR
                     TVFGSLERFMGILIEHYAGSFPFWLAPVQVRIVPVKEDHIPYAAEVESRLKEIGLRVE
                     KDIRDEKLGKKIRDAQVEKIPFMVVLGAREVENGTLSVRERSEGDLGTMDLSGFEELI
                     RRQPDPVSGRRSTCTC"
     gene            5765..>6084
                     /gene="infC"
                     /locus_tag="PK146_03590"
     CDS             5765..>6084
                     /gene="infC"
                     /locus_tag="PK146_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPE91356.1"
                     /translation="MDDQGVKVGVVPIAQALELAEERELDLVEVSGQADPPVCRILDY
                     GKYKFQQQKRDKDAGKKSKGQVVKEMKMRPKIDLHDYNFKVRAIKDFLLSGFRVKVAV
                     FFRG"
BASE COUNT         1453 a         1597 c         1875 g         1159 t
ORIGIN      
        1 tctccccggt gaagaggaat tcccctatgc ggtaagggtg gtatcggaca tccttgaatc
       61 caacggctcc agttccatgg cctcggtatg cggtgccagc ctctcgctca tggatgcagg
      121 agtgccggta aggaagcccg ttgccggcgt cgccatgggt ctaataaagg aaggggaaaa
      181 ggtggcgatc ctcaccgaca tccaggggct ggaggatcat tacggggata tggacttcaa
      241 ggtcacgggg accagggaag gcatcacggc cctccagatg gacaacaagg ccggcggcat
      301 aaccaggagc atcctggaaa gagccctgga ccaggcaagg aggggcaggc ttttcatcct
      361 tgaaaagatg gagagtgcca taacagaacc ccgggataac gtctcggcct atgctccaag
      421 gatctacacc cttacggtcc cactggacaa gatcagggac attatcggcc cgggcggcaa
      481 gatgatcagg aacatagtct cccagactgg cgtcaaggtc gacgtggagg acgacggaag
      541 ggttttcgtg gcctctccga cgacggaggc cgcagagaag gccctcggta tgatcaacga
      601 tctcatcagg gaagtgacgg cgggagaggt cttctccggc aaggtgacca ggctgatggc
      661 cttcggagcc ttcgtggagg tcctgccagg caaggagggg ctcctgcacg tgagcgagat
      721 cagcactacc catgtgggca aggtcgacga cgttttcagc tccggtgact cggtccttgt
      781 gatggtcaag gagatcgacg acatggggag gatcaacctc tccaggagga gagcccttga
      841 aaggaagaac gagcccgaac tggccgaaag gttcgccgat gccttcgttc tggaggatgc
      901 cagggaagag aggatcaagg ctctgccccc atctggcgac cgtgacagga accagagggg
      961 aggagctccg agacctggtg gaagaccggg cggggacaga ccttccggcg acagaagatc
     1021 ccacagtggg ccccgcagga gtagttccgg ggacaggaac aggtagccat gcaggaagtg
     1081 gtcaggctcg gtatcttccg cgacgatgaa gatattgccc tgccggtcta cgctacaccc
     1141 ttctcgtcag gggtcgatct aaggtccaac gaaagggtca ccatccccgc cggtgggaga
     1201 tcggcggtgg ggaccgggat ccggctcgag atacccgaag gatacgagtg ccaggtgagg
     1261 ccccggagcg gcctggccct gaagaacggt gtgaccgtgc tgaatgcacc cgggacgatc
     1321 gatgccgact acaggggtga agtgagagtg atccttgtca acctgggtga aaaccccttc
     1381 gagatagaga agggggacag gatcgcccag atgatcctgg cacctgtcag caggatcgaa
     1441 tgggaggaga ctgactctct gagcgagacg gaccggggca gcggcggttt cggaagcacg
     1501 ggaaggtagg atctcaaaaa aggcattgaa cgtaacaggg ggccgtccat aggggcggcc
     1561 ccctgttctt gctctatcgc ttgtttcccg ggactatttc ttctcctcgg ctttcatggt
     1621 cctgtagacg caccagccgg tgccgccgaa gacaagggcc attatgacta tcatgttgaa
     1681 ctgccaaccc atcggaatcc ctccctgtaa gtcgtcttgc tcaaacagaa accggtagcg
     1741 ggactgggag ccgttctatt cggtagcctc gaactttacc gtcgacttgt cggccagcca
     1801 gttgtttgtt accagggcga gtacaaccac gatcgcccac tggtagacca tagtacccac
     1861 agtgaaggtg tactccgaga tggggagcca cttcatgtag gtgtcggggt accagcttgc
     1921 ggcctgcgcg atccaccacc agaagatgat cacgaaccat accgggaaga ggtagataag
     1981 cttgaacatc catggagcat tcaggtcgga atcgggggcg atgtactggt cccagatctt
     2041 gttgacgccg atcttcattg ctgcgatgga gaagatcaga ccgctcagga gaagacctac
     2101 gccccagacc cagtcctggt tgtccaggaa ggagatgctc caggctgagg gcaggccaag
     2161 aacaaggatg ccgattcctg cagctatggc tgccttgttg cgtgtccagc cggcatccat
     2221 caggagacgg aggccgagtt cgaccatgga taggagcgaa ctgaagcccg caaagataag
     2281 ggccaggaag aagatggagg cgaagatgac ccctccgggc ataacagtga aaaggcttgt
     2341 gaggtggatg aatgcgagcc cggtgttccc gcttgcaacc gctgccatgg gatccggcga
     2401 aagagcgaag atggtgggga ttatcgccag accggccagg aagccggcca tggtgtcggc
     2461 gaagcatacg gtggtggcgt tgagggcaat gtcctcacgc ttcttggtgt agacgaagta
     2521 ggtgagcatg agtccccagc ccgcccccgt ggaccatgcg acctgggtga aggcctcgag
     2581 ccagacgcgg cccttgagga ggtcggccca cttgggctcg aagaagtatc tcagaccttc
     2641 agtggatccg gggagcatgt tggccctgat ggccaggaca acgagcatga caaaaagggt
     2701 gggaatgagg accttgttga tcttctcgag accctgggag atgcccttga aaaggatcac
     2761 tgtggcggcg accatggtca ggatatgcca cggtaccatc gcgcctggtg tgccggagaa
     2821 agcggcccag agagcttccg tatcaagccc tggtttgatg acccccgtta tggcgtacag
     2881 aaagtaccgg atgcaccatc caaccacaac ggagtagtag aaggcgatcc caagagagat
     2941 gacggcgacg gtggtaccca tccaggtcca ctttctgcct gccatctcct tgaaggaccc
     3001 tatgacgccc atccttgata ccttacccca gacggcttct gccatgagga gcggaatagc
     3061 ccagaggaac agtccgatca aaagagcgat gacgaaagca ccgccaccct gggaggccgc
     3121 gactcgcgga aaccgccaga tgttacccgt acctactgcc ataccaatgc aagagagaat
     3181 aagaccccat cgggtagacc actggtcggc ctttttgatc tcggccatgc tttgaatccc
     3241 tccttggtga gagtttttgg aacggaagat cctgttctgg gaaaacatga atgtggtatt
     3301 tttaactatc ttccctgttc caccccctcc acgggaaaat gaacaaaagg aaaaaagtaa
     3361 agaaaggttt aaaccgatta taggagaaca aggttctttt ttccagaaaa aataaggcgt
     3421 tgccatacgc ccccgggagt gtatattata tacactacag ggaagggtga cctcgctgtc
     3481 ctgctgttgt agaatgcaat ccacctgacc tttgtcgcag gagcgtcagg gatgggtcat
     3541 ttttcaagat ggaggacttt aaattgccta attattcagg tcctgaagga ttggaactaa
     3601 actcctccga gcctctgaag gcccgggaga tactgtcgag atggggactt tctgaaggat
     3661 ccattgccgc cgtcgttgac gggacacgga tcgatcttgc agctgtggtc aagtctgact
     3721 ccctcgtaga gccagtgctc tcttcaagcc ccgaaggact ggagatcctg agacactcca
     3781 catcgcatct gatggcccag gccgtccaga ggctctttcc cggaacaagg ctggggatcg
     3841 gaccctcgat tcaggacggt ttctactacg acatggagat agccggccag atcaccgaag
     3901 aggaccttcc caggatcgag gaggagatga ggaagatctc ctccgaggat attccagtgg
     3961 agaggctcct cctgcccagg gaagaggccc tggatctttt cagcgaaaga gatgccgtct
     4021 acaaggtcga actggtctcg gagatccccg acgagctcat ctcgctctac agacagggcg
     4081 acttcgttga cctctgcagg ggcccccacg tgatctccac ctcgcagctg aagcatttca
     4141 agcttctttc cttggcagga gcctactggc gaggtgacga gaagaacata atgctcacga
     4201 ggatctacgg gacggccttc gacaccgccg aggccctgga tgatcacatt aacaggatag
     4261 aggaagcaaa acgcagggac cacaggaaac ttggtagaga gcttgacctt ttcagcatcc
     4321 aggaggaggg accggggttc cccttcttcc accccaaggg tatggtgatc ataaaccgac
     4381 tcgtggattt ctggcgggcc gagcactccc gaaggggata cgctgagata aggacgccgc
     4441 tgatactcga ccaggacctg tggataaggt cgggccactg ggaccattac cgtgagaaca
     4501 tgtacttcac cgagatcgac gagcgcccct tcgcggtgaa gcccatgaac tgccccgggg
     4561 ggatcctggt ctacaagagc agaataagga gctaccgtga atttcccatg aggatagccg
     4621 agctcggtac cgttcaccgc cacgagcgtt cgggggtcct gcacgggctg atgcgggtaa
     4681 ggtcctttac ccaggacgac gcccaccttt acgtgacacc ggaacagatc aagcaggaga
     4741 tcatcgggat catggacctg gtcaggttca tctacaggga cgtattcggc ttttcatacc
     4801 gggtggagct ttccaccagg cccgagaagg ccatgggaga cccggtaatg tgggagaagg
     4861 ccgagaaggc cctgcaggag gcccttgagg atttcgggac cgatttcagg gtgaatcccg
     4921 gcgacggagc gttctacgga cccaagatcg acttccacct cgaggactgc atcgggagga
     4981 cctggcagtg cggcacgatc cagctcgact tccagatgcc cgaaaagttc gatatgacct
     5041 acatcggtcc ggacgggaac gagcatcgcc cagtaatgct ccacaggacg gtctttggta
     5101 gtcttgaaag gttcatgggg atcctcatag agcactatgc cgggtcgttc ccattctggc
     5161 tggcaccggt ccaggtaagg atcgtgccgg taaaggagga tcacatcccc tacgccgccg
     5221 aagttgagtc aaggctcaag gagatcggtc tcagggttga gaaggatatc agggacgaaa
     5281 agctgggcaa gaagataagg gacgcccagg tggagaagat ccccttcatg gtcgttctgg
     5341 gcgccaggga ggttgagaac gggaccctgt ccgtaaggga acggagcgag ggcgaccttg
     5401 gaaccatgga tctttctggt ttcgaggaac ttatcaggag gcagcctgat cctgtcagcg
     5461 ggcgaaggag cacttgcacc tgctagttgt tatgtgctag aatcccttgt cggtcccttt
     5521 ccgggacaaa aaaggaataa cgatgaagag gagccaagcc tcccacccgc cgacgccttg
     5581 atgggctgtt taagggtata tatgccgggc cgcaagatga ggttttgcgc tccggatcat
     5641 atgggatata gaggccgggc tcgatgagtc cggccatttt ttaccgtgga ggtgacgagt
     5701 tatagctaaa aacagcgaag aaccgcgcat caacgaagag atccgggcaa aggaagtcct
     5761 tctgatcgac gaccagggcg tcaaggtcgg agtggtcccc attgcgcagg cgctcgagct
     5821 tgccgaggag agggagctgg acctggttga ggtctcgggc caggccgatc ctcctgtctg
     5881 caggatcctt gactacggca agtacaaatt ccagcagcag aagcgtgaca aggacgcagg
     5941 aaagaagtcc aaggggcagg tcgtaaaaga gatgaagatg agacccaaga tcgacctgca
     6001 cgactacaac ttcaaggtgc gcgccatcaa ggatttcctc ctttcgggct tcagggtcaa
     6061 ggttgccgtc tttttccggg gcag
//
LOCUS       DAOPKQ010000070         5863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_14638_length_5863_cov_4.658402, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000070 DAOPKQ010000000
VERSION     DAOPKQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14638_length_5863_cov_4.658402"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PK146_03595"
     CDS             <1..639
                     /locus_tag="PK146_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HPE91357.1"
                     /translation="ALRVDTVVVSAQHHPAVESGQIREDIIREVITPVLPGHLTETPP
                     RILVNPTGRFVMGGPMADTGLTGRKIIIDTYGGVVPHGGGAFSGKDPTKVDRSAAYMS
                     RYAAKNVVAAGLAGECKIQVAYAIGVARPVSLMVDTNGTGKLSDTVLTRLIQEHFDFR
                     PAAVIRDLDLRRPQFRRLAAYGHMGRIDLDPIPAWERIDRAEILRKAAEKSC"
     gene            646..1350
                     /locus_tag="PK146_03600"
     CDS             646..1350
                     /locus_tag="PK146_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPE91358.1"
                     /translation="MTRELTPHRGIWRSRLERAAGVLLHLLWPVTCPVCGRPGSIICR
                     ECILEVVEKGPGRECCSECLRPFPCADHPAAFPIKGLTTFGGLSRELVHSLKYGSRKC
                     LGKPMGRLIGERIRPAGAVALVPVPLHRGSERAFNQSLEIAKGISGEWVCPVADRLAW
                     RERRPSQVGLSAGLRKKLPRDSIAWKGKVDSDLPVVLVDDVCTTGATLRAAAGAVRAA
                     GARVEGALVWALAGIS"
     gene            complement(1365..1592)
                     /locus_tag="PK146_03605"
     CDS             complement(1365..1592)
                     /locus_tag="PK146_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPE91359.1"
                     /translation="MDCIATFDTTHMAILFEKTCRNAGFSCKIVPVPRSISASCGLAC
                     RFRVEDETMIRQLAMEHSIEVLEYHPPDPGI"
     gene            1699..2136
                     /locus_tag="PK146_03610"
     CDS             1699..2136
                     /locus_tag="PK146_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPE91360.1"
                     /translation="MSRSRKILSVMALSALVLTALSGIALAQEKIGVIDPQKVLFQHP
                     KFEQAQKQIKTVVEKKQAEAKDAIDKTSDNNEKQRIFEAKRQEAAREEQKIMEPIFKD
                     IDLAIRTVAKAKGVTIVLDKTQVFFGGADVTEDVIQELKKKNA"
     gene            complement(2208..2939)
                     /locus_tag="PK146_03615"
     CDS             complement(2208..2939)
                     /locus_tag="PK146_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91361.1"
                     /translation="MTGKKEPLLSITDLHVSYGAIRAIRGVNLDVFEGEVVCVIGANG
                     AGKSTLMNAVMGNVRREKGILLLEGKPLANRSYQVVSQGVSLSPEGRKVFAPLTVIEN
                     LMMGAFPNKDRSKIQGDLEWVFTLFPRLRERRDQHAGTLSGGEQQMLSIGRALMSKPR
                     LLLLDEPSLGLAPIIIRDIFKELKRINEEGVTILLVEQNARQALLLSHRGYVMQTGRI
                     LMEGPASELLKNPDVEAAYLGTSRK"
     gene            complement(2939..3715)
                     /locus_tag="PK146_03620"
     CDS             complement(2939..3715)
                     /locus_tag="PK146_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91362.1"
                     /translation="MADPILRLENLNKHFGGVHAVKDITFSLEQGELAGLIGPNGAGK
                     TTVFNLITGVYSPDSGQILFKGEDIASRKTYEVVAKGIARTFQNLRLFPRSTALENVM
                     TACQQHFRYNFFEALTHLGRWRNNENSIRKMSLDLLERVGLLPVAEQAAGTLPYGHQR
                     RLEIARALALQPELLLLDEPAAGMNPEEVHVLNGLILNIHRDFNLTTLVIEHHMEVIM
                     EICPHIVCMNFGEKIAEGDPDHIQNNPEILKAYLGEEVEE"
     gene            complement(3708..4589)
                     /locus_tag="PK146_03625"
     CDS             complement(3708..4589)
                     /locus_tag="PK146_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE91363.1"
                     /translation="MSGYLEGIIILICINSIAAMGVSLLTGFTGIFTLGHAGYMAIGA
                     YTAAILTVRHHVPWLVAVLAAGTLAMVIAYLIGVPTLKLMGDYFAIASIGLGETIRLI
                     LENWQSFTRGARGFPGIANYTTLPVAAAFFLGMLVLMRFLINGNYGRAFKACRDDFVA
                     ASLLGFKTAHYRVLSLAISGFYCGIAGGLMAGFLTYLQPIMFDMQKSTELTAVVVFGG
                     LGSLSGSIIGTTVIGIVTEVFRPISQYRMLIYGAIMVIIMVARPEGIMGQHEVGPKLF
                     RSLFSRKTVNEGDGANG"
     gene            complement(4600..5481)
                     /locus_tag="PK146_03630"
     CDS             complement(4600..5481)
                     /locus_tag="PK146_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE91364.1"
                     /translation="MDTLLQQVINGLSLGSVYALIAVGYSLVYSILLFSNFAHGGFLV
                     IGGYICYYTLAAVGHNIWVASLLALLGAGASAVLVEKVAYKPIRERTNVTLYLLIASM
                     GMSIVIENIFVVTVGGRFRALPPVIPMNPVNVFGLATTSAFDILSLVMAILFLSGLQI
                     FLSRTKWGLAIRAASYDLRTAGLMGVNVNKLISIVFFVAGLLAAVGGIFLSVRYTLYP
                     QLGLITIKAFVAAVIGGLGSLPGAVVGSLILGLAEMLTAGFISSQMRDIVVFGMLVLT
                     LLWRPTGLFGKHVAEKV"
     gene            complement(5576..>5863)
                     /locus_tag="PK146_03635"
     CDS             complement(5576..>5863)
                     /locus_tag="PK146_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE91365.1"
                     /translation="DFFANYKKTYNDECKEFVNGILAYDSIYWLADAIERAGKVDSTA
                     IKDALENTVNVQLHHAVMSIDPATHNPMNKTGVVLVAKDGRAQFFTRIQPE"
BASE COUNT         1257 a         1651 c         1766 g         1189 t
ORIGIN      
        1 gccttgaggg ttgacactgt ggtcgtatcg gcccagcatc acccggccgt agagtcgggg
       61 cagatccggg aggatatcat cagggaggtt ataacccccg ttcttccagg ccacctgacg
      121 gagacccccc cgaggatcct ggtgaacccc accgggcgct tcgtcatggg cggtcccatg
      181 gccgatacgg ggcttacagg aaggaagata ataatagaca cctacggagg ggtggtacct
      241 catgggggcg gcgcattctc cggcaaggat cccaccaagg ttgaccgatc tgccgcctac
      301 atgagccgct acgccgcaaa gaacgtcgtt gccgcggggc tggccgggga gtgcaagata
      361 caggtcgcct acgcgatcgg agttgcaagg cccgtttctt tgatggtgga taccaacgga
      421 accggaaagt tgtccgatac tgtcctgaca aggctcatac aggagcactt cgatttcaga
      481 cccgctgccg ttatcaggga cctggacctc aggagacccc agttcaggcg acttgcggcc
      541 tacggacata tggggaggat agacctggat cccatccctg cctgggagag gatcgacagg
      601 gccgagatcc tcaggaaggc cgccgaaaag tcctgctgac aaggtgtgac gagagagttg
      661 acgccccaca gggggatatg gagatcgcgc ctcgaaaggg ccgcaggtgt tcttctgcac
      721 cttctttggc cagttacctg tcccgtatgc ggaaggccgg gttcgatcat atgccgggag
      781 tgcatcctgg aagttgtcga aaaggggccc gggagagagt gctgttccga atgtctcagg
      841 cctttcccct gtgccgatca ccccgcggcc ttcccgataa aggggcttac caccttcggg
      901 gggctttcca gggagctggt acacagcctg aagtacggat ccaggaaatg cctggggaag
      961 cccatgggac gtcttatagg ggagaggatc aggccggcgg gagctgtcgc cctggtccct
     1021 gtcccccttc acagggggag cgaaagggcc ttcaaccagt ccctggagat agcgaaaggc
     1081 atctccggag agtgggtctg cccggtggcg gacaggctgg cctggaggga aaggcgccct
     1141 tcacaggtgg gactctctgc agggctcagg aagaagcttc ccagggattc tatcgcctgg
     1201 aagggaaaag tcgacagtga tcttccggtg gtacttgtgg acgatgtctg caccaccggc
     1261 gcgacgctcc gggcagccgc cggggctgtc agggcagcgg gggcaagggt agagggggcc
     1321 ctggtctggg cccttgccgg gatctcctga tggagaggag agggtcagat accgggatcg
     1381 ggggggtggt actccaggac ttctatcgag tgctccatgg ccaactgcct gatcatggtc
     1441 tcatcttcga ccctgaaacg gcaagccagc ccgcagctgg ccgaaatgct cctcgggacg
     1501 ggcactatct tgcaggagaa gcccgcgttc ctgcaggtct tttcaaagag tatcgccatg
     1561 tgggtggtat cgaaggtggc gatgcagtcc atcagtcggc ctccggtctc ttcgttttta
     1621 tgtaattata cctagacgag acgggtttgt aaggatagaa tatttacctg atcgagtgaa
     1681 ttcaaaggag gttcttaaat gtctcgctca cggaagatcc tttcggttat ggcacttagt
     1741 gctctggtat tgactgccct ttccggtatc gccctggccc aggagaagat cggcgttatc
     1801 gatccccaga aggtcctttt ccagcatccc aagttcgagc aggcccagaa gcagatcaag
     1861 accgtagtcg agaagaagca ggccgaagcc aaggatgcca tcgacaagac aagcgacaac
     1921 aatgagaagc agaggatctt cgaggccaag cgccaggaag ctgccaggga agagcagaag
     1981 atcatggagc ccatattcaa ggatatagat ctcgccatca ggaccgttgc caaagcgaaa
     2041 ggtgtgacca tagtccttga caagacccag gtgttcttcg gaggtgccga cgtcaccgag
     2101 gacgtcatcc aggaactcaa gaagaagaac gcctagctgc acaacctcag aacaggaaaa
     2161 ggtccgggcc gtcacggccc ggaccttttt tcatccctca ggatctgtca ttttctggaa
     2221 gtgcccaggt aggcggcctc cacatcggga ttcttcagaa gttccgaggc gggaccctcc
     2281 atgaggatcc tccccgtctg catcacgtac cccctgtggg agagcagcag cgcctgccgt
     2341 gcgttctgct cgaccagcag gatcgtcact ccctcctcgt ttatcctctt cagttccttg
     2401 aagatgtccc tgatgataat cggcgccaat cccagggagg gttcgtccag aagcagtagc
     2461 cggggtttcg acatgagcgc ccttccgatg gagagcatct gctgctcccc gccggagagg
     2521 gtgccggcat gctggtccct tctctccctg agcctcggga agagggtgaa gacccactcc
     2581 aggtccccct ggatcttgct cctgtccttg ttggggaaag cccccatcat aaggttctcg
     2641 ataacggtaa gaggggcgaa gaccttcctc ccctcggggg agagggaaac cccctgggag
     2701 accacctggt agctcctgtt ggccaggggc ttgccttcga gaagcaatat tcccttctct
     2761 ctcctgacgt tgcccatgac ggcgttcatc agcgtggact ttccggcccc attggccccg
     2821 ataacgcata ctacctcgcc ctcgaagaca tcaaggttga cccccctgat agccctgatc
     2881 gccccgtagc tgacgtgaag gtccgtgatg ctcaggagag gttctttctt gccggtcatc
     2941 actcctcgac ctcctctccg agataggcct tcagtatctc cgggttgttc tggatgtggt
     3001 ccgggtcgcc ttccgcgatc ttctcgccga agttcatgca gacgatgtgg gggcagatct
     3061 ccatgatgac ttccatgtgg tgttcgatca ccagcgtggt caggttgaaa tcccggtgga
     3121 tattcaatat caggccgttc agtacgtgga cctcttcggg gttcattccg gcggcgggct
     3181 cgtcaagaag aagaagttcg ggctgaagcg ccagcgccct tgctatctcc agccgcctct
     3241 ggtggccgta aggaagcgtc ccggcggcct gttccgcaac ggggagaagt ccgacccttt
     3301 ccagcaggtc gaggctcatc ttccttatgc tattctcgtt gttcctccat cttcccaggt
     3361 gggtcagggc ttcgaagaag ttgtacctga agtgctgctg gcaggcggtc atgacattct
     3421 caagcgccgt tgagcgaggg aagagacgca agttctggaa ggtcctcgct atccccttgg
     3481 ctacaacttc gtaggtcttg cgggacgcga tgtcctcacc cttgaaaagg atctgccctg
     3541 aatccggtga atatacgcct gttataaggt tgaatacggt ggtcttgccg gctccgttcg
     3601 ggccgataag tccggcaagc tcgccctgct cgagcgagaa agtaatatcc ttgacggcgt
     3661 gcacgccgcc gaagtgcttg ttcaggttct ccaggcggag gatgggatca gccattggct
     3721 ccatctccct cattcacggt ctttctggag aacagggacc tgaaaagctt cgggcctacc
     3781 tcgtgctgac ccatgatccc ctcgggcctg gcgaccatta ttatgaccat gatggcaccg
     3841 tagatgagca tgcggtactg ggagatgggc ctgaagacct cagtcactat ccctatcacg
     3901 gtggtcccga ttatggaacc gctcagggag cccagccccc cgaatacgac caccgcggtc
     3961 agctccgtgg atttctgcat gtcgaacatg atcggctgga gataggtcag gaagcccgcc
     4021 atcagtccgc cggcaattcc gcagtaaaac cctgaaatgg caaggctgag gaccctgtag
     4081 tgagccgtct tgaaacccag cagggaggcc gcgacgaagt cgtcacggca ggccttgaaa
     4141 gccctcccgt agttgccgtt tatcagaaac ctcatcaaga caagcatccc cagaaagaac
     4201 gccgcggcta cggggagggt ggtgtagttc gctatacccg ggaaacccct tgcgcccctg
     4261 gtaaaggact gccagttctc caggatgagc cgtatcgtct ccccgaggcc gatagaggca
     4321 atggcgaagt agtcccccat cagtttcagg gtggggaccc cgatcaggta ggctatgacc
     4381 attgccaggg tcccggcggc caataccgct accagccagg gaacatgatg ccggacggtg
     4441 agaatagccg cagtataggc gcctatcgcc atgtatccgg cgtgacccag ggtaaagatg
     4501 ccggtgaaac ctgtgagaag gcttacaccc atggcggcta tggagttgat gcagataaga
     4561 attattatcc cttcaagata tcctgacatg ggaagcctcc tagacctttt cggcaacgtg
     4621 cttgccgaaa agcccggtag gtctccagag cagcgttagc acaagcatgc cgaagacgac
     4681 gatgtccctc atctggctcg atatgaaccc ggcagtgagc atctctgcca gacccaggat
     4741 cagactgccg accactgcgc caggcaggct ccccagccca ccgatgactg cagcaacgaa
     4801 agcctttatc gtgataagcc ccagctgggg gtagagggtg tagcgcacgg agaggaatat
     4861 cccgccaacc gcggcaagca gaccggcaac gaaaaagacg atggagatga gcttgtttac
     4921 gttgaccccc ataaggcccg ccgtcctgag gtcgtaggat gctgctctta tggccaggcc
     4981 ccacttggtc ctggataaaa agatctggag gcccgagaga aagagtatgg ccattaccag
     5041 ggaaaggatg tcgaaagcgc tggtcgtggc gaggccgaag acgttcacag ggttcatggg
     5101 gatgacgggc ggaagggccc ggaaacgccc cccgacggta acgacgaaga tgttctcgat
     5161 cactatgctc atccccatgg aggcgatgag aaggtagagg gtgacgttgg tcctttcccg
     5221 gatgggcttg taggccacct tctcgaccag gactgcagaa gctcccgccc ccagaagggc
     5281 gagaaggctt gccacccaga tattgtgccc caccgccgca agggtgtagt agcatatata
     5341 gccgcctatt accaggaaac ctccgtgggc gaagttggag aagaggagaa tggagtaaac
     5401 cagggagtag ccgacggcaa taagggcata gaccgatccg agggaaagcc cgttgatcac
     5461 ctgctgcaga agagtatcca aaacgggttc accgtccttg tccactgcaa atccggggcg
     5521 gacgcggaac ccgcgtccgc cccggttcgg gttactttca aaaaagaggt gatgtctatt
     5581 ccggctggat ccttgtgaag aactgcgccc ttccgtcctt ggccacgagc acgacgcccg
     5641 tcttgttcat cgggttgtgg gtcgcggggt cgatggacat cacagcgtgg tggagctgaa
     5701 cgttaacggt gttctcgaga gcgtccttga tggccgtgct gtcgaccttg ccggcccttt
     5761 cgatggcgtc ggcaagccag tagatggagt cataggccag tatcccgttg acgaactcct
     5821 tgcactcgtc gttgtaggtc ttcttgtagt tggcgaagaa gtc
//
LOCUS       DAOPKQ010000071         5842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_14714_length_5842_cov_3.576119, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000071 DAOPKQ010000000
VERSION     DAOPKQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14714_length_5842_cov_3.576119"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..850)
                     /gene="rpe"
                     /locus_tag="PK146_03640"
     CDS             complement(146..850)
                     /gene="rpe"
                     /locus_tag="PK146_03640"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPE91366.1"
                     /translation="MAGPLPVKSWKRPIIAPSLLSADPLDIGGSVDLLQGEQDWLHLD
                     VMDGHFVPNLSYGPALIEACRSRWPDFVLDVHLMVDRPEDFVEDFARAGSTVLTVHQE
                     ATPHLHRVLHRIRDLGCSPGVTINPGTPVENIKPVLDLVDLVLVMSVNPGFGGQGFIP
                     GSLEKTRLLCCWREAFGHDYLIEIDGGIGPGNSAQAVKGGCDVIVAGSAVFNTPEPSV
                     AVREIRARALEGVICD"
     gene            complement(829..1917)
                     /locus_tag="PK146_03645"
     CDS             complement(829..1917)
                     /locus_tag="PK146_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPE91367.1"
                     /translation="MKRLFKITLAILILVIAGSGIFAFYTIFFGGKDVAVPGLAGSPI
                     VDAVSLTEKMGLLVRIDKVDSVQPAGEVVSQWPEPGSRVKKGKIVILKVSKGGDRQPL
                     PDVRGMEYGQAVTRLQESGFDVGDVVRIHSDSRPAGVVAAQSPASPVMVPPGRRIDLL
                     VSLGPPRTGGKIVVPDVLERSEKIARQIIVQSGLKVGTVKYEYTYMTPAGMVMNMDPK
                     PGRTLSPEGVVNLVVATGKKPEEPAAPAAVAQEEPETVVSFPTGGIPAPPVEPGPTMV
                     QETPAAPQTTPTPSPGDKIARIRYQVPPLAQSMPVRIEIVDKNGTRVLLDRQALAGEY
                     ISLDAPYEGQSSVTIYLGGEFVWQDRYQ"
     gene            complement(1929..3257)
                     /locus_tag="PK146_03650"
     CDS             complement(1929..3257)
                     /locus_tag="PK146_03650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HPE91368.1"
                     /translation="MKGRGIEASLLVWRDVLAGGFASEALRKRTETLPETERTLAATL
                     VFAALRRAALWKHMAREMTRRPLGGMSKAAGDALVIGIAGVCELKTFSPRVLVNALVE
                     WVKERGEEREPGMVNAVLRRAAREGPDIVARLRESRSIKDASLYSGVPGWAAGLWTGS
                     WGKELAKEIIRHSSMKTFLSLRLSGRQEPTAVLKALEGAGLRAWRSPLVRQSIRMSSS
                     GHPPSLPGYREGAFTPQTESSMIVGDIASSIFQGGSVLDMCTGRGVKAFQYLESVPGS
                     FLEGWDLSAPRINAALLESERLGVGRERFAFRKGDALEMEPLASPELVMLDAPCSGSG
                     TWARHPDGKMRLSPEKLKDLAYLQSKLLVRAIDIAAPGGTVLYSTCSLFRQENEQVVA
                     KAMEDRPGIVEMPPHNTYDCLIRGRPWGNYILPRLPWLDGFFIAVLTKRA"
     gene            complement(3254..3949)
                     /locus_tag="PK146_03655"
     CDS             complement(3254..3949)
                     /locus_tag="PK146_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HPE91369.1"
                     /translation="MPLILFALILLFFLPWLGLLVLALLLFLFLLVPLGFAARSLAWL
                     VVGPRELYRVLSNSRVRKNHALEHGTVNILKERYGIYGLSGMAMEDGFSLSGFPDPRI
                     ILEAAETARKRLAAGETHLAIDRRCGTTLVMVNLFAAAIFILLLVLAGRFSFGTVLLS
                     LAAAWVLGPLTSPLVQRYVTTDSRVEDLAITGVETRPRPVRFPGGRALLPSEVFIHTR
                     NAGEPLVAEVAGP"
     gene            complement(4073..4762)
                     /locus_tag="PK146_03660"
     CDS             complement(4073..4762)
                     /locus_tag="PK146_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPE91370.1"
                     /translation="MFPFFFDPTFLVLIPALILAVWAQARVKSAFQRYSEVRARRGVT
                     ARDAVRTILDSNGLSGVPVERVQGALTDHYDPRKKVLRLSESVYGSPSIAAIGVAAHE
                     AGHAIQDVRGYAPLKLRNAFVPVASLGSSLALPLFFIGFLFANPTMMDLGIIFFAAVL
                     LFHLVTLPVELDASKRALSILSDSGLLASDEIGGAKAVLNAAALTYVAATLMSALQLV
                     RLFLLRGMRNR"
     gene            complement(4863..>5842)
                     /locus_tag="PK146_03665"
     CDS             complement(4863..>5842)
                     /locus_tag="PK146_03665"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPE91371.1"
                     /translation="LHATGCASGTDALLLALMALEVGPGDEVITTPFSFFATVSCITR
                     VGATPVFADVEPGTYNIDPEAVLAKITPKTKAIIPVHLFGQMARLEEFAPRLREQGIG
                     LVEDCAQAIGATRIIDGKVRRAGAVGEIGCFSFFPTKNLGCFGDGGMVTVSSDELRTR
                     VRSLRVHGSGKQYFHDEIGLNSRLDELQAAILRVRLPHLETWNEERRLVAERYRILFA
                     EHDLDEWIMPPIEEKGNRHIFHQYVIRCRSRDQLKDHLAQRGVTARVYYPLSLHLQPC
                     FSFLGYSKGDMPVSETLTEEVLALPIFPELRPEEQERVVKAMAEFYHGK"
BASE COUNT         1203 a         1797 c         1656 g         1186 t
ORIGIN      
        1 caggtcctgc agggtaccgt tctcgatggc ctcaaggaac tgcacgcgaa tcttggtcct
       61 cccggaaatg tccgaaaggc tgagcccctt gcgccggcgg cggccgccgg cctccttccc
      121 caactgttcg gcgttccttg tttcttcagt cacagatgac tccttccagg gctctggccc
      181 tgatctccct gaccgctact gagggctcgg gagtgttgaa gacagcgctg ccggcaacga
      241 tcacatcgca gccgccttta actgcctgtg ccgaatttcc aggtcctatt cccccgtcaa
      301 tctctatgag gtagtcgtga ccgaaggcct ctctccagca gcaaagaagc ctggtctttt
      361 ccagcgaccc cgggataaag ccctgtcccc cgaaacccgg gttcaccgac attacaagca
      421 cgagatcgac aaggtccagg accggcttga tgttctccac tggtgtaccg gggttgatcg
      481 taacgccggg ggaacacccc aggtccctta ttctatggag aacccggtga agatgcggcg
      541 tcgcctcctg gtgtaccgta agcacggtag atccggctct tgcaaaatct tcaacaaaat
      601 cttccggcct gtccaccatg agatgtacgt caaggacgaa gtctggccaa cggctcctgc
      661 aggcttcgat caaggccggt ccgtaggaca ggttgggtac gaaatggccg tccattacgt
      721 caagatgcag ccagtcctgc tccccctgaa gcaggtccac cgacccccct atgtcaaggg
      781 ggtccgccga gagaagcgaa ggggctatga taggcctctt ccaggacttt actggtagcg
      841 gtcctgccat acgaactctc ctccgagata aatggtgacc gacgactgcc cctcgtaggg
      901 ggcatcgagg gagatatact cccccgccaa agcctgcctg tcgaggagga ccctggtgcc
      961 gttcttgtcc acgatctcta tcctcaccgg catggactgg gcaaggggcg ggacctggta
     1021 tcttatcctt gcgatcttgt cccccgggga gggtgtcggc gttgtctgcg gggcggcagg
     1081 agtctcctgg accatcgtcg gaccaggttc tacgggaggc gcaggaatac cgcccgtcgg
     1141 gaaagagacc accgtttcgg gttcctcctg ggccacagcc gcaggcgcag caggctcttc
     1201 aggtttcttc cccgtggcaa ccacgaggtt caccacacct tccggggaca gggtcctgcc
     1261 cggcttggga tccatgttca tgaccatccc cgccggggtc atgtaggtat attcgtactt
     1321 tacagtgccg accttcagac cgctctggac tatgatctgt ctggcgatct tctcgctcct
     1381 ctccaggaca tcggggacaa cgatcttccc tcctgtcctg ggcgggccaa ggctgaccag
     1441 aaggtcgatc ctcctcccgg gcggcaccat cactggagat gcagggctct gggcagcgac
     1501 gaccccggca ggcctgctgt cgctgtggat cctgacaacg tcaccaacgt cgaaacccga
     1561 ttcctgcagc ctggtcaccg cctgtccata ctccattccc ctgacgtcgg ggaggggctg
     1621 gcggtcgccc cccttgctca ccttaaggat cactatcttg cccttcttga ccctggatcc
     1681 aggctccggc cactgggaga caacctcgcc ggcaggctga acggagtcga ccttgtctat
     1741 ccttaccaga agccccatct tctccgtcag gctgacggca tcaacgatag gggagcctgc
     1801 caggccgggg acagccacgt cctttccccc gaagaagatg gtgtagaagg cgaagatccc
     1861 cgatcccgcg atgacaagga tcaggatagc aagagtgatc ttgaaaagtc ttttcatctt
     1921 ggatcctcct atgccctttt ggtcaggacc gctatgaaga aaccgtccag ccacggaaga
     1981 cggggaagaa tgtaatttcc ccacggcctt cccctgatga gacaatcata tgtgttgtgg
     2041 ggcggcattt caacaatccc ggggcgatcc tccattgctt tggcgaccac ctgttcattc
     2101 tcctgcctga aaagactgca ggtagagtaa aggaccgtgc ctcctggagc tgcgatatcg
     2161 atcgccctga ccagcagctt tgactggagg taagcaagat ccttcagttt ttccggtgaa
     2221 agcctcatct tcccgtcggg gtgcctggcc caggtgccac tcccggaaca gggggcatcg
     2281 agcatcacca gttccgggct ggcaaggggc tccatctcaa gggcgtcccc cttcctgaaa
     2341 gcgaacctct ccctgccgac cccaagtctt tccgactcca gaagggcggc attgatcctt
     2401 ggggccgaga ggtcccagcc ttccagaaag ctacccggga cactttcaag gtactggaat
     2461 gcctttaccc cccttccggt acacatatcg aggaccgatc ccccttgaaa gatggaagag
     2521 gcaatatcac cgacgatcat ggatgactcc gtctggggcg tgaaggctcc ttcacggtaa
     2581 cccggaagtg aaggggggtg gccgctggag gacattctga tgctctgcct gacaagaggg
     2641 gacctccagg ccctgagtcc ggctccttcc agggccttca ggaccgccgt gggttcttgc
     2701 cttccggaaa gcctcagtga gaggaaagtc ttcatcgagg agtgcctgat gatctccttt
     2761 gccagctcct ttccccagga cccggtccat agtcccgcgg cccacccggg aacaccggag
     2821 tagaggcttg catccttgat ggaacggctc tcccttaacc tggccacgat atcgggaccc
     2881 tccctcgccg ccctgcgaag aacggcattg accatccccg gctcccgttc ctctcctctc
     2941 tccttgaccc actccacaag ggcgttgaca aggacccgag gcgagaaggt cttcagttcg
     3001 cagacgcctg cgatgcctat cacgagcgca tctccggcag ctttgctcat cccccccagg
     3061 ggcctccggg tcatctccct ggccatgtgc ttccagaggg cagcccttct caaggctgca
     3121 aagaccagcg ttgccgcaag ggtcctctca gtctcgggaa gggtctcggt cctcttcctg
     3181 agggcttcac tggcgaagcc cccggccagg acatctctcc agaccagaag cgaggcctct
     3241 atgcccctcc ccttcatggt cctgccacct cggccacgag aggctctcct gcgttcctgg
     3301 tatggatgaa gacttcagat gggagcaggg cccttcctcc agggaagcgg accggccttg
     3361 gccttgtttc gaccccggtt atggcgagat cctcaaccct gctgtcggtg gtgacgtatc
     3421 tctggaccag agggctcgtt aaaggaccga ggacccacgc tgccgcaagg gacagtagga
     3481 cagtaccgaa agagaacctg ccggcgagga ccagcaggag aatgaatatg gccgccgcga
     3541 agaggttcac cattaccagt gtcgtcccgc accttctgtc aatggcgaga tgggtctccc
     3601 ccgctgcaag tctcttcctg gctgtctccg cagcctcgag aatgatccgg gggtcaggaa
     3661 acccgctcag cgaaaagcca tcttccatgg ccattcctga aagaccgtaa atgccgtatc
     3721 gctctttcag gatattaacc gtaccgtgct ccagggcatg attcttcctg accctgctgt
     3781 ttgagagcac cctgtagagt tccctgggcc cgacgacgag ccaggcgagg gaccttgcag
     3841 cgaatcccaa agggaccagt aaaaagagaa agaggagtag cgccaggaca agaagtccga
     3901 gccacggaag aaagaacaga aggatcagcg cgaacagtat caggggcatg gatcatattt
     3961 ctcctctcgg gccggcttgt ccttacccgg agggcaagga cagaacaggg ggtggctgaa
     4021 gccgccccct aatgatacat caacagccag aagccgggga aagctgaata gatcagcggt
     4081 tcctcatgcc tctcagcagg aagagcctca ccagttgaag ggctgacatg agggtggcag
     4141 caacgtaggt tagggccgcg gcgttaagaa ccgccttggc gcctcctatc tcgtcactgg
     4201 caaggagccc agaatctgac aggatggaga gggccctttt gcttgcgtcc agctccaccg
     4261 gaagggtgac caggtgaaaa agcaggaccg ccgcaaagaa gatgatgcca aggtccatca
     4321 tggtggggtt tgcgaacaga aaccctataa agaagagggg cagcgcaagg gaggacccca
     4381 ggctggccac aggaacgaag gcgttcctca acttcagggg ggcgtaacca cggacatcct
     4441 gtattgcgtg cccagcctcg tgggccgcaa cgccgatggc ggctatgcta ggactgccgt
     4501 atacgctctc tgaaagcctg aggaccttct ttctcggatc gtagtgatct gtcagggcac
     4561 cctggaccct ttcgacagga acgccggaaa ggccgttcga gtccagaatg gtccttaccg
     4621 catcgcgggc ggtaacaccc cgtcttgccc tcacctcgct gtacctctgg aaggcgctct
     4681 ttactctggc ttgcgcccag actgcaagta tcagcgccgg gatcaagaca aggaaagtag
     4741 gatcaaagaa gaacgggaac ataccgacca cctccgtctc ccccaggggg aagaccccat
     4801 tggatatctt ttccgaacct tattttaccc catctggaag ggtaaaatca agaaaggtca
     4861 aactattttc cgtgatagaa ttccgccatg gctttcacga cccgttcctg ttcctctggc
     4921 ctgagctcgg ggaagatagg gagagccagg acctcctctg taagggtctc ggagacgggc
     4981 atatcgcctt ttgaataacc caggaaagag aagcagggct gaaggtgcag cgacaggggg
     5041 tagtagaccc tggctgtgac acccctttgg gcgagatggt ccttcaactg gtccctcgaa
     5101 cggcaccgta taacgtactg gtggaagatg tgcctgttcc ccttctcttc aatggggggc
     5161 attatccatt cgtccagatc atgctctgcg aagaggatcc ggtacctctc cgctacaagt
     5221 cgcctttctt cgttccaggt ctcgaggtgg gggagcctga ccctcagaat ggcggcctgc
     5281 agctcgtcga gcctgctgtt gagtcctatc tcgtcatgaa agtactgttt gccggagccg
     5341 tggaccctca ggctccggac tcttgtccgg agttcatccg atgatacggt gaccatgcct
     5401 ccatcgccga agcaaccgag gttcttggtg gggaagaagg agaagcagcc gatctctccg
     5461 acggcgcctg ccctccggac cttgccgtca atgatcctgg tcgctccgat ggcctgggca
     5521 cagtcctcga caaggccgat cccctgctct ctcagtctcg gggcaaactc ctccaacctt
     5581 gccatctgtc cgaagaggtg gacagggatg atcgccttcg ttttcggggt tattttcgcg
     5641 agaaccgctt ccgggtcaat attgtaggtc ccgggctcga cgtcggcaaa gactggggtc
     5701 gctcccaccc tggttatgca gctaacggtt gcgaaaaaac tgaagggcgt cgttatgacc
     5761 tcgtcgccgg gccccacctc cagagccata agggccagga ggagggcatc agtgcctgaa
     5821 gcgcaccccg tggcatggag tg
//
LOCUS       DAOPKQ010000072         5840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_14721_length_5840_cov_6.274503, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000072 DAOPKQ010000000
VERSION     DAOPKQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14721_length_5840_cov_6.274503"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK146_03670"
     CDS             complement(<1..364)
                     /locus_tag="PK146_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPE91372.1"
                     /translation="MDDHRMTAPVAAALVSVYFFWGTTYLAMKFAIETLPPFIMLGLR
                     FTTAGAIMFVWEWIRLRPRISWGQWRGMAMVGALMLLGGTGGVAWSEQVVPSNIAAIV
                     VATVPLWIAIIRWVYLRHD"
     gene            complement(457..1872)
                     /locus_tag="PK146_03675"
     CDS             complement(457..1872)
                     /locus_tag="PK146_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE91373.1"
                     /translation="MRERKLFQRIFLWFLGAMLLMVLVSVLLTVFMTQQGIILTGQQE
                     ALSNALDRNGKGLLEVVETGNPARLRESIDRIRSETGLGIFIFDVDGVPVPVEGGPSK
                     DFLERLGRDAADLLGQGTTFQTRGEFSSVFRQLGSPSGKDYILVGVFRRSPYIARVLT
                     GNPKALLIHLMGLLATALVFCFLLARYLSDPLVRLSKTARSVSSGQLDTPVEDLVKDR
                     SDEIGELARSFEAMTKHLTTLLEAQRRLIRDISHELRTPLARLGVALELARKRSGTVA
                     RESLERIERESEVLNDMIGDLLSLSKVEAEMDHVTFEMVDLAAILENVVEDANFEARV
                     IKRRVELVYPDDLEPIRLNSELVRRAVENIVRNGLRYTPEGTAVSVKAERRSRGGRPG
                     VSLTVLDKGPGVPEDELQQIFKPFYRTETSRSRDTGGSGLGLAIAQKAALAHGGEINA
                     ELPSGGGLLVEMWLPLQSMDL"
     gene            complement(1872..2576)
                     /locus_tag="PK146_03680"
     CDS             complement(1872..2576)
                     /locus_tag="PK146_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE91374.1"
                     /translation="MERILLADDDTGLCELLTEFLESEGFKVESIHDGRKVASRVLAE
                     RFSLIILDVMLPGLSGMEILKEIRRQSAVPVLMLTARGDDVDRIVGLELGADDYLPKP
                     FNPRELVARIRAVLRRGRMDSSRSSQTGTYHVGDMTIDPAARYVSAGGKRIELTTVEF
                     DLLETLAHSAGTAISREVLVRSVLKRSFSPFDRSLDVHISNLRRKIGTFSDGSDRIKT
                     VRGLGYILALPTEESG"
     gene            complement(2695..3066)
                     /locus_tag="PK146_03685"
     CDS             complement(2695..3066)
                     /locus_tag="PK146_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPE91375.1"
                     /translation="MKQEIRTDRSPDPIGPYSQGLKVGNRIYVSGQGPLNHETGKTPE
                     TIEEQTRQVLTNIRNILEAGGATMDDVVKTTVHLADLKDFQAFNSVYKEFFSPPFPVR
                     TTVGSQLLGILVEIDVIAEIQ"
     gene            complement(3163..4854)
                     /gene="ilvD"
                     /locus_tag="PK146_03690"
     CDS             complement(3163..4854)
                     /gene="ilvD"
                     /locus_tag="PK146_03690"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPE91376.1"
                     /translation="MTWRSKRLGLFSGRGSASRRAIYKGCGYDDEDLSRPLIGVVNTA
                     NDAGLGHVHLDRLAARVKAGILQAGGTPFEFSTIATCGAVPIGTPHLRYELVIRDVIA
                     SSVEIMANVQLLDGLVLLASCDSIIPGVLIGAIRSGLPSIFLSGGPQLVTRFEGRQAV
                     MSELDQLVFGAQYGDEDVSAKLRYLEDNVCPGPGACALMGTANTMQILAEGIGMSLPG
                     SATVPAVYAEKERFATRTGRRIVDMVKEDLTPRKIISRENLLNGITLTMGIAGSTNAV
                     LHLLSLARDLDIDLDLDDFDRISREVPVISRVIPTGKATVVDLHHAGGVPAIMGELGE
                     YLHGGALTVSGLRAEDIFSKERSKDLSVLTTADKPVLANGGIAVVRGNLAPDGAICRT
                     STISEKALRFKGPARVFDSDEEAHSAVVEGKISKGDVVVIRREGPRGAPGMREMMMTT
                     DALVGLEMGQEVFVVTDGRFSGFTEGAAVGHLSPEADVGGPIAVVREGDPISIDIPAR
                     RVDLDIPAEELKRRLSEWIPPQPKVSRGILGLCAKTALQAHLGAMLEDRVSNPKA"
     gene            5468..>5840
                     /locus_tag="PK146_03695"
     CDS             5468..>5840
                     /locus_tag="PK146_03695"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HPE91377.1"
                     /translation="MKGRARHAKVFLAMMQILDQKAKPEARLLDLSANSRMADEQYAL
                     TRGKYEKITRKYFREDGTLLPFTLKARERVALPRKIVEPFGQGVRYSKKELNTLLAKI
                     AEDYVLVRRCLVDHGFVSCRAD"
BASE COUNT         1301 a         1690 c         1557 g         1292 t
ORIGIN      
        1 ggtcgtgtcg aagatagacc cagcggatga tggctatcca caatggaaca gtagccacaa
       61 ctatggctgc aatatttgag ggcacgacct gttccgacca ggcaactccc ccggtcccac
      121 ccagaagcat cagggctccg accatggcca tacccctcca ctgtccccag gagatccggg
      181 gtctgagacg tatccattcc caaacgaaca tgatcgctcc cgccgtagtg aacctgagtc
      241 ccagcataat gaagggcggc aaggtctcta tcgcgaattt catggccaga tacgtggtcc
      301 cccagaagaa ataaactgac accagggccg cggctacagg cgcggtcatg cgatgatcgt
      361 ccaagaataa tctcaccttt tccaagtctt gtttgtcaaa accagtcaat tatagacggt
      421 gatggaaaga tctgcttatt gaagggaatt tctttttcac aggtccatgg actggagagg
      481 caaccacatt tccaccagga gaccgcctcc cgacggaagc tcggcattga tctctccacc
      541 gtgggcaagg gcagctttct gggctatggc aagaccgagg ccggaacctc ccgtatcacg
      601 ggatcgggat gtttctgtcc tgtaaaaggg tttgaatatc tgttgaagtt cgtcttcagg
      661 taccccgggg cccttatcca gaaccgtcaa agagacaccg ggccgccccc ccctgctccg
      721 tcgttctgcc tttacggaaa cagcggtgcc ctcgggggtg tacctgaggc cattgcggac
      781 tatgttctcc acggcccttc ggaccagttc gctgttgagt cttatcggct ccaggtcatc
      841 ggggtagaca agctcgaccc ttcgcttaat gacccgcgcc tcgaagttgg cgtcttctac
      901 aacattttcc aggatagccg caagatcgac catctcgaag gtcacgtgat ccatctcagc
      961 ctcgaccttt gacagtgaga gcaggtcgcc gatcatatcg ttcaggacct ctgattctcg
     1021 ctctatccgt tcgagggact cccttgctac agtccccgat ctcttcctgg cgagctccag
     1081 tgcaactcca aggcgagcca ggggggtgcg gagttcatgg gatatgtccc ggatcaggcg
     1141 ccgctgagct tccaacaggg tcgtaagatg ttttgtcatg gcctcgaagc tgcgggcaag
     1201 ctcgcctatc tcgtcggatc ggtccttgac caggtcctcc acaggggtgt ccagttggcc
     1261 actcgaaacg gacctggctg tcttgctcag ccgcaccagg gggtccgaaa ggtacctggc
     1321 cagcaggaag cagaaaacta gagcggtggc taaaaggccc atcaggtgga tcagcagggc
     1381 cttggggttc ccggtgagga cccttgcgat gtagggtgat ctccggaaaa cccccacaag
     1441 aatgtagtcc ttgccagaag gagaaccaag ttgcctgaag accgacgaga actcacccct
     1501 ggtctggaaa gtggtaccct gaccgagaag atccgcggca tccctgccca gcctctccag
     1561 aaaatctttc gagggaccac cttcgacggg tacaggtact ccatctacgt cgaagataaa
     1621 tattccaaga ccagtttcgc tcctgatcct gtctatggac tctcttaggc gggcggggtt
     1681 ccctgtctcg accacttcga gcagtccttt accattcctg tcgagagcgt tcgaaagggc
     1741 ttcctgctgc cctgtaagta ttatcccctg ctgggtcatg aagaccgtca gaagaacgct
     1801 gacaaggacc atcaggagca tggcccccaa aaaccacagg aatatcctct ggaacagctt
     1861 tctctctctc actatccaga ctcctcggtc ggcagggcga ggatatagcc cagccccctg
     1921 acggtcttga tccggtcaga gccgtcagag aaggtgccta tcttccgtcg gaggttgctg
     1981 atgtgaacgt caaggctcct gtcgaagggg gagaaggacc tcttcagtac acttctgacc
     2041 agaacctctc tcgagattgc agtgccggcc gaatgggcca gggtctcaag aaggtcaaac
     2101 tccacggtgg taagctcgat gcgtttaccg ccggcgctca cgtacctcgc cgccgggtct
     2161 atggtcatgt cccctacgtg gtaggtgcct gtttgggaag aacggctgct gtccatccgg
     2221 ccccgcctca gtacggctct gatccgggcc accagttccc tggggttaaa gggtttgggg
     2281 aggtagtcat cggctccaag ttcaaggcca actatccggt ccacatcatc gcccctggct
     2341 gtcagcatca ggacaggcac agcggattga cgacggatct ccttcaggat ctccattccc
     2401 gaaagaccgg ggagcattac gtcaaggatg atcagcgaga aacgttctgc gagaaccctg
     2461 gatgccacct tgcgtccatc gtgaatggat tctaccttga atccttcgga ctcaaggaac
     2521 tcggtaagca attcgcaaag cccggtatca tcatcggcta gcagtatacg ctccacggca
     2581 atccccccat gacaggaagg tctttgtcag actacctcat tgatccctca actgctgtca
     2641 agaaaggttt tctcttgtaa agggtcctta acattccttt acagtggaca ccgatcactg
     2701 gatctcagcg atcacatcta tctccacaag gatccccagc agttggcttc ccacggtggt
     2761 cctcaccgga aaggggggag aaaagaactc cttgtagaca ctgttgaagg cctggaaatc
     2821 cttcaggtcg gcgaggtgaa cggtggtttt cacaacgtcg tccatggtcg caccgccggc
     2881 ctccaggatg ttcctgatgt tggtcaggac ctgacgggtc tgttcctcga tcgtctccgg
     2941 ggttttgccg gtttcgtggt tcagcggccc ctgtcctgac acgtagatgc ggttacccac
     3001 tttaaggccc tgggaatagg gaccgatcgg atctggcgac ctgtctgttc ggatctcttg
     3061 cttcatcttg aaaacctccc taaaaaagat ggtgaacggt gaacggaaag atctgcagga
     3121 aaacaaaggt tttgccgaat tcagccgttt actgtttact gtttacgcct ttgggttact
     3181 taccctgtcc tccagcatcg ctcccaggtg agcctgaagt gctgtctttg cgcagagacc
     3241 cagtattccc cggctcacct tgggttgcgg cggtatccac tccgaaagcc gccttttcag
     3301 ctcttcagcc ggaatatcga ggtccactct tcgtgccggg atatcgatgg atatgggatc
     3361 cccttcccgg acgacggcta tgggtccccc cacatctgct tctggtgaaa gatgcccaac
     3421 tgcggcgccc tcggtaaaac cggagaagcg cccatcggtg accacgaaaa cctcctgacc
     3481 catttccaga ccgacgagtg cgtcggtggt catcatcatc tccctcatcc ccggggcccc
     3541 gcgtggtcct tcacgccgga taaccaccac gtcccctttg gagatctttc cctccaccac
     3601 agcggaatgg gcttcctcgt cggaatcaaa gaccctggct ggacctttga aacgaagagc
     3661 tttctcagat atagtcgagg tccggcagat ggctccgtcg ggggcaaggt tgcctcttac
     3721 gacggcgatt cccccgttag caaggacagg cttatctgcc gtggtcagaa ccgaaaggtc
     3781 tttcgatctc tctttactga aaatatcctc cgccctcagg ccggatacag tgagggctcc
     3841 gccatggaga tactctccca gttcacccat tatcgcgggt actccccccg cgtggtgaag
     3901 gtcgacaacg gttgccttcc ccgttggaat gacccttgat atcacaggga cctcccggga
     3961 tatccggtcg aaatcgtcca ggtccagatc gatatccaga tcccttgcca ggctgagaag
     4021 gtgcagaacg gcatttgtgg aaccggctat acccatagtc agtgttattc cgtttaagag
     4081 gttctccctg gagatgatct tccggggggt cagatcctcc ttgaccatat cgacgatcct
     4141 gcgtcctgtc ctggtggcaa atcgctcctt ctcggcataa acggccggaa ctgtcgccga
     4201 tcccggaaga gacatcccta tgccctctgc caggatctgc attgtgttgg ccgtccccat
     4261 gagggcacag gcacccgggc cagggcatac gttatcctca aggtaacgca gctttgctga
     4321 cacatcctca tcgccgtact gggcgccgaa aacaagctgg tccagttcgc tcatcacggc
     4381 ctggcggcct tcgaaacggg tcacgagctg gggccccccg gaaaggaata ttgaagggag
     4441 gcccgagcgg atagcaccta tgagtacccc gggaattatg ctgtcgcatg atgccagcag
     4501 gaccagcccg tcaagaagct ggacgttagc cattatctcc accgatgatg ctataacgtc
     4561 ccttatgacc agttcgtagc gaagatgcgg agtcccaatg ggcaccgccc cgcaggtggc
     4621 tatggtgctg aattcaaagg gtgtgccccc ggcctggagt atcccggcct ttacacgcgc
     4681 cgcgagcctg tcgagatgca cgtgccccaa ccctgcgtcg tttgcggtat tcaccacacc
     4741 gatcagcggg cgagagaggt cctcgtcatc gtatccgcaa cccttgtaga tcgcgcggcg
     4801 cgatgccgac cccctgccgc tgaaaagtcc aagtcttttt gatcgccagg tcatctttga
     4861 tccgttcctt tcatgcattt gatggtccta tgccaaactt ataccatgac ccatgcattg
     4921 cttgcactct ctccggataa accggtctag gagctgaggc aaaattataa taataaggaa
     4981 cccctgttat gacgatctgg aaaagatcct gcaacacgag ccggcgggaa tgacattacc
     5041 gctaacatgt tcaggcatta tatcttctga ctgtacatgc cttttattaa acaaccaaag
     5101 aagatggacg ttaacaaatc taccctttga atcttccaaa tagcatacat agaagagctg
     5161 gctgaaggcg ctttgtagga tcctgggacg atggcgaagg tttgcctgaa gtgtgtgaag
     5221 acctttgcgg tgggggggct tccagttggg agaccgctaa ttggaggccg aagccgcggc
     5281 aaggatccat tccgaaagtg aacacggacc tcagttcaac tacctgctga acctcatgaa
     5341 gcagtagaag ggcgtctccg ccccccagac aaaactgctt gaggcgttct tccggggtga
     5401 taacgacaag aagatcgctt cggaactcgg gataagccgc tccacagtca ggaatcaccg
     5461 tttccgcatg aagggacgtg ccaggcacgc aaaggtcttt ctggcaatga tgcagatcct
     5521 cgatcagaag gcaaagccgg aggcaaggct cttggatctt tccgccaact cgaggatggc
     5581 cgatgaacag tatgccctta ccaggggaaa atacgaaaag ataacacgga agtacttccg
     5641 ggaagacggt accctgttgc cttttaccct aaaagccagg gagcgggtcg cacttcccag
     5701 aaagatcgtg gagccctttg gacagggagt cagatactcg aaaaaggaac tgaacaccct
     5761 ccttgcaaag attgccgagg attatgtcct tgtgaggagg tgcctggtgg atcacggctt
     5821 cgtcagctgt cgcgccgacg
//
LOCUS       DAOPKQ010000073         5824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_14787_length_5824_cov_4.817646, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000073 DAOPKQ010000000
VERSION     DAOPKQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14787_length_5824_cov_4.817646"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3347)
                     /locus_tag="PK146_03700"
     CDS             complement(<1..3347)
                     /locus_tag="PK146_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPE91378.1"
                     /translation="MSDRTGLDLRQGLETLLGEVAAISRVFFAGFDIGSAAIHCVVVN
                     DGNSVVFSPPPNLHQGSPLHSLRRSLREILDLFPGGSLVSTAFTGIGSLHFPEAFPGI
                     LREFDSVCVVRGARFVEPECEAILHLGARDACFFHVTGDRFNPVILDWSTSSKCGAGS
                     GTLLEKQVRRLFPSCGERPGSLEGTLEMAASEASRHPEAPPYNARCGVVLQSDLIHDQ
                     NEGLPRDLIMARLFRTIASNIVQDVIGNRDIRQGTRSVLTGWPAGCAPLFENIKNLTS
                     LDLVRPDNFANVGAIGAALLAHEKDNRFVIEENRIDEAARIARSKRQYLPPLRESLER
                     VHIHGFAPSPLSDREGASGGPEVVIGVDGGSTTTKAVALSLADRRILGSRYIPTHGDP
                     LGALAEIMGHLSKTLGELPVSGVCTTGSARQLYERVLSSPSLRKTLESRGCLVPDGAV
                     DEITCHAVGIRSMDDRIDTIFEIGGQDMKFTTFRREEDSVTDQVDEARMNYSCQAGAG
                     QTLENMSTILGLDVRDSLQELAMEADRVPVIDATCGVFMELEEQRLIAENHSTGEIAA
                     AITRATASSYFNKFVGGTRHVGERCSCQGGPSLGKAFLAAMADVTGRDIHAYPGRELM
                     GALGAALFIRERILSARGEGQETASAFRGWGAASESFSHLDTTCPEHFGARSCGKREC
                     KLRIYSIAGEEIVTGGFCPLGNSEGSGSARPDHVGIFHSILEKHFQGAQFDALEREAR
                     DTRPRIGIRRCTTTIGGRAIWSSALFSSLGFLPVLSPISDERISRLGLRNSPTDFCVA
                     MKLSAGHTALLAADPGIEHIFIPSLIRRTRKTGPSHMFCIYTEAEGFLPEDDLGLPEG
                     KVIRPVWHLGNRKQMARSLERELFRVGYHFTMQEIQDAFRKADTFEEAFNKDIARLGD
                     AFLETLSRNGERGYVGIGRDYVVLDPAASSSTGRMFATVRGMPFIPQVFLRHLFSDIP
                     IDDLVENEYWEHSSEILKASIFTARHERLFPVRQMNFACGPDSIKFIMEDAIFRRAGK
                     PFLHLLTDAQTNNAPFVTRAEAFERVASRWQPKETPLERFSFVRRSPDGIEGRRWLVP
                     WMGNASTLGAAAAKYWGIDAIVAPTDTP"
     gene            3448..4917
                     /locus_tag="PK146_03705"
     CDS             3448..4917
                     /locus_tag="PK146_03705"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPE91379.1"
                     /translation="MRIVATDRDPSQWRGDALVLFIIEGKMDPVDQLGEEIASLVKQK
                     ASRLRFTGKEDSLLAVDTPGGIVGSIYLAGLGSADGASPEPWRRGIALVVRQAGKDRH
                     RRILILPGGAATLEVHSALAEGALLSEYSFDRYKSGLDPSEKPEPIEELLVHGGDERS
                     LARGSMNAIAQNFARDLVNEPGNVINPAGLAERASAMAGECGLECSIFDEEKMREMGM
                     NGILSVGAGSHNAPRLIHLVYRPSGPSRERIAFVGKGLTFDSGGLNIKPGDYMRSMKG
                     DKTGACNVLALMKAVAESKPDLEIHGFAGAAENMPGGGAYRPDDIIRMFNGKTVEVDN
                     TDAEGRIVLGDVLAYASSQKPAVMIDMATLTGACVVALGPYTAGILGNDTALVASFRE
                     ASGRTGERVWELPMDDERLKKKIRSEVADVANNGGRYGGAITAAMFLREFVGEGIKWL
                     HVDIAGVDSSDEEYGYYRKGVTGFGVRTCLEWLSKYYKQ"
     gene            complement(5251..>5824)
                     /locus_tag="PK146_03710"
     CDS             complement(5251..>5824)
                     /locus_tag="PK146_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPE91380.1"
                     /translation="NSGPGSPAFLYVNRRHFGRMPALAGWFGYDKARQFDMNIEFVPA
                     GTAGAWQISSPSILGSASVRGALRVTLEAGIDSIRAKSLAMTSFFMRLVDEFLSREPY
                     NFRIGTPREPERRGGHVALEHPTEALRINEALIARGVIPDFRPPQVIRIAPIALYNTF
                     TEVWQVAMHLKGIIDNREYEKFSKVRKAVS"
BASE COUNT         1184 a         1659 c         1723 g         1258 t
ORIGIN      
        1 ggcgtatcgg tgggcgccac tatggcatct atcccccagt attttgcagc ggccgctccg
       61 agggtgctgg cgttgcccat ccagggtacc agccatcttc ttccctcgat cccgtcaggc
      121 gatctccgga cgaaggagaa cctttccaga ggtgtctcct tcggctgcca ccgggaggcc
      181 accctttcaa aagcctcggc cctcgttaca aagggagcgt tgttggtctg ggcatcggtc
      241 agcaagtgca ggaagggttt tcccgccctt ctgaagatag cgtcctccat gatgaatttg
      301 atggagtccg gaccgcaggc aaagttcatt tgtctgacag ggaaaagcct ttcatgcctc
      361 gcggtgaaga tggaggcctt gaggatctcg gagctgtgct cccagtattc gttctccacc
      421 aggtcgtcga tgggaatatc cgaaaagaga tgtctcagga agacctgtgg gatgaagggc
      481 atccccctga cggtggcgaa catcctcccc gtagaggatg aagcggccgg atccaggacg
      541 acgtaatccc tgcctattcc aacgtacccc ctttctccgt ttcgggagag ggtctccagg
      601 aaggcgtccc ccagccgggc gatatccttg ttgaaagcct cctcgaaggt gtcggccttg
      661 cgaaacgcat cctgtatctc ctgcattgtg aagtggtaac ccaccctgaa aagctctctt
      721 tccagtgatc ttgccatctg tttcctgttc ccgaggtgcc agacggggcg tatgaccttt
      781 ccctccggaa gaccaaggtc gtcctcgggg aggaaaccct cggcctcggt gtagatgcag
      841 aacatgtgtg agggacctgt cttccttgtc cgccttatca ggctcgggat gaaaatgtgt
      901 tctattcccg ggtcggctgc cagaagcgcc gtgtgacccg ccgaaagctt catggctacg
      961 cagaaatccg tgggggagtt ccttaatcca agtcttgaga tcctctcatc cgagatgggc
     1021 gagagcaccg gcaaaaaccc cagcgacgaa aagagggctg aagaccagat cgccctcccg
     1081 cctatggtgg tcgtgcatcg ccggatccct atccggggcc tggtgtcgcg cgcctctctt
     1141 tccagggcgt caaactgggc tccctggaag tgtttttcca ggatggagtg aaagatcccc
     1201 acgtgatctg gtcttgcgga tcctgatcct tcgctgttcc ccagtgggca gaaacctccc
     1261 gtcacgatct cctctccggc aatggaatat atcctcagtt tgcactcccg tttaccgcag
     1321 gaccttgctc cgaagtgctc cgggcaggtc gtatccagat ggctgaaact ctcggaggcg
     1381 gcgccccacc ctctgaaggc ggaggcagtc tcttgccctt cccccctggc cgaaaggatc
     1441 ctttccctta tgaaaagggc agccccgagg gctcccatga gctccctgcc ggggtaggca
     1501 tggatatccc ggcctgttac gtccgccatg gctgccagga aggctttccc aagcgatggg
     1561 ccgccctggc aggaacatct ttcgcccacg tgccttgtgc cccccacgaa cttgttgaaa
     1621 tagctcgaag ccgtcgccct cgttattgcg gcggctatct ccccggtcga gtggttctca
     1681 gcgatcagcc gctgctcctc gagttccatg aatacgccgc aagttgcgtc gatcaccggg
     1741 acgcggtccg cctccatggc cagttcctgg agcgaatcac ggacatccag ccccaggata
     1801 gtggacatgt tctccagcgt ctggccggct cctgcctgac aggagtagtt catcctggcc
     1861 tcgtccacct gatcggtgac actgtcttcc tcacgcctga aggtggtgaa tttcatatcc
     1921 tgtccgccga tctcgaagat ggtgtcgatc ctgtcatcca tggaccttat tcccacggcg
     1981 tgacaggtta tctcgtcgac ggccccgtcg gggacgagac accccctgct ttccagggtt
     2041 tttcggaggg aagggctaga aaggaccctc tcgtagagct gcctggccga accggtggtg
     2101 cataccccgg acacgggcaa ctctcccagg gtctttgaaa ggtgtcccat gatttcggcg
     2161 agggcaccca gagggtctcc atgagtgggt atatacctgc ttccgaggat ccgacggtct
     2221 gccagcgaaa gggctactgc ctttgtggtt gtggagccgc cgtcgactcc tatcaccacc
     2281 tcgggtccgc cggaagctcc ttcgcgatca ctcaggggag aaggggcgaa accgtggata
     2341 tgaacccttt ccagtgattc ccttagaggg gggaggtact gtcttttgga gcgggctatc
     2401 cttgcggcct cgtcgatcct gttctcttct atcacaaagc ggttgtcttt ttcgtgggcc
     2461 agcagcgccg ccccgatggc cccaacgttg gcgaaattat cgggtcgtac caggtccagg
     2521 gatgtcaggt tcttgatgtt ttcgaaaaga ggtgcacagc ctgccggcca accggtcagt
     2581 accgatcttg tgccctgtct gatatcccgg ttgccgataa cgtcctgaac gatgttcgac
     2641 gcaatggtcc tgaagagcct cgccatgatc aggtctctgg ggagaccttc gttctggtcg
     2701 tgaataaggt ccgactgtag aactacaccg cacctggcgt tgtatggagg cgcttcaggg
     2761 tgcctcgagg cttcggatgc tgccatttcc agagttcctt ccagggaacc gggtctctct
     2821 ccgcaggacg ggaagagcct tcggacctgt ttttcaagca gggtccccga accggccccg
     2881 cacttgctgc tggtactcca gtcgaggatc acggggttga acctgtctcc ggtaacatgg
     2941 aagaagcatg cgtccctggc ccccaggtgg agaatggctt cgcactcagg ctccacgaac
     3001 cttgcgcccc ggactacgca gacgctgtcg aactccctga ggatccccgg gaaggcttca
     3061 ggaaagtgca gtgagcctat accagtgaag gccgtcgaga ctagtgaacc tcccgggaac
     3121 aggtcaagta tctctctcag ggatcttctg agggagtgga gggggctacc ctggtgaagg
     3181 ttcggaggag gcgaaaagac gacagaatta ccgtcgttca ccactacaca gtgaatggct
     3241 gccgatccta tatcgaaccc ggcgaaaaac accctgctta tggccgccac ctcccccaga
     3301 agcgtctcta acccctgacg caggtcgagg cctgttcggt cagacaatta taccttgaaa
     3361 ggctttcacg gggtttcact ccgcagggcg aaaatatgat attgttgccc cgtcgacaag
     3421 caagaaaaat gcggtggagg tgcggcaatg cgtatagtgg cgactgaccg ggacccctcc
     3481 cagtggagag gcgacgcact ggtccttttt atcattgaag ggaaaatgga ccccgtggac
     3541 cagcttggag aagagatagc ctccctggtc aagcagaagg cctcgaggct cagatttaca
     3601 ggcaaggagg acagccttct tgcagtggac actcccggag ggattgtcgg tagtatttac
     3661 cttgcggggc tgggaagcgc tgacggcgcc tctcctgaac cctggagaag agggatcgcc
     3721 cttgtggtac gacaggccgg caaggatcgg cacaggagaa tactgatcct gccaggcggg
     3781 gcggcgactt tggaagtaca ctcggcgctg gccgaaggag ctctcctttc ggagtacagt
     3841 ttcgacaggt ataaatcagg acttgatccg tcagaaaagc ccgaacccat cgaggagctt
     3901 ctggttcacg ggggcgatga gcgttccctt gcaagaggtt caatgaacgc cattgcccag
     3961 aacttcgcca gggacctggt caatgagccg ggcaacgtta tcaacccggc cggattggct
     4021 gaaagggcct cggcaatggc aggggagtgc gggctcgaat gcagcatctt tgacgaagag
     4081 aagatgcggg agatgggaat gaacggcatt ctgagcgtcg gtgcagggtc ccataacgct
     4141 ccgagactga tacacttggt ctaccgccct tcaggcccgt ccagggagcg tatcgccttc
     4201 gttggcaagg ggcttacctt tgacagcggt ggtctgaaca taaaacccgg ggactacatg
     4261 agaagcatga agggtgacaa gacaggggcc tgcaacgtac tggccctgat gaaggccgta
     4321 gcggagtcga aaccggatct agagatccat ggcttcgccg gtgccgccga gaacatgccc
     4381 ggaggcggcg cctaccggcc ggacgatatc atcaggatgt tcaacggcaa gaccgttgag
     4441 gtggacaata ccgatgccga ggggaggatt gttcttgggg atgttcttgc ctacgcttcc
     4501 agtcagaagc ctgctgtcat gatcgacatg gccactctga ccggggcctg cgttgtggcc
     4561 ctggggcctt acacagcagg gatcctcggg aacgacaccg cccttgtcgc ctccttcagg
     4621 gaggcatcag gcaggacagg cgagcgggtc tgggagcttc ccatggacga cgaacggctg
     4681 aagaagaaga tccgctcgga agtggccgat gtggccaaca acgggggaag gtacggcggg
     4741 gcaataacgg ctgccatgtt cctcagggag ttcgtcggcg aggggatcaa gtggctgcac
     4801 gtggacatag cgggggtaga ctcctcagac gaagagtacg gatactaccg caagggtgtc
     4861 accggcttcg gcgtccgcac ctgcctggag tggctaagta aatactataa acagtgaacg
     4921 gaaagatcaa atgctaaacc tttgcaccgc agagaacgca gagttgcaca gagaaatgca
     4981 aaaactggtt cgtgttcagg agccaaacct gaattcaata ctggtatgga tgatgattac
     5041 agtttatgaa ggttaaaagg gttcggtttt ccttatgcct gtgtcctgtc accttagtct
     5101 tggcctttgc ctctcacaaa taaaggtttc ctctgcggcc ctctgtgtcc tacctgacgc
     5161 tgacggagcg tcagcgtcgg gcaccttgtc tgtggtgcaa cggtctgtct ttgatttgtg
     5221 tgtttacacc aatcgaggcg gctttgccgc ctaagatacc gccttgcgga ccttcgagaa
     5281 cttctcgtac tccctgttgt cgattatccc cttcagatgc atcgccacct gccagacctc
     5341 ggtgaaggtg ttgtagaggg ctatcggagc gatcctgatc acctgtggtg gtctgaaatc
     5401 gggtatcact ccgcgggcaa tgagggcttc gtttatcctg agagcctccg tcgggtgctc
     5461 cagggctaca tggccccccc ttctctccgg ttcccttgga gtgcctattc ggaagttgta
     5521 gggttctctg gaaaggaatt cgtcgacaag cctcataaag aaggaagtca tggccagaga
     5581 ttttgccctt atgctgtcta tgcccgcctc gagcgtgacc cgcagggcac ccctgaccga
     5641 ggccgacccg aggatcgacg gggatgatat ctgccaggcg ccggctgtcc ctgccggtac
     5701 gaactcgatg ttcatatcga actggcgcgc cttgtcgtag ccgaaccacc ctgccagagc
     5761 cggcatcctg ccgaaatgcc tccggttcac gtagaggaag gcaggggatc cgggtcccga
     5821 gttc
//
LOCUS       DAOPKQ010000074         5810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_14841_length_5810_cov_5.041703, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000074 DAOPKQ010000000
VERSION     DAOPKQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14841_length_5810_cov_5.041703"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK146_03715"
     CDS             complement(<1..284)
                     /locus_tag="PK146_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPE91381.1"
                     /translation="MAAVTWAVAPILYRRGVERISYSGLGALRCTGYIISAALFLFFA
                     MGPSAFAPLAAGTMARIFVYSVLWLVMGDFFYFSALHKLGVSIAVPVTSS"
     gene            complement(324..1016)
                     /locus_tag="PK146_03720"
     CDS             complement(324..1016)
                     /locus_tag="PK146_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE91382.1"
                     /translation="MQNDPLSPARNIDLRQIVYEKIKEAIVEGIIRPGERLSEVDLAD
                     KLAVSRTPVREAIRQLAQTGLVTLEPRRGAFVSLPTIKDASDLYDLRTHMEIIAVRLV
                     TLNQPIEELEYWRPVFSAMTDQTETSHYLAEDRKFHASLYELSRNRYLTMMLHNIADL
                     INLCRHYSVEGIPLSTFAYEHVAIIDAIETGDPDKAEAELRAHLERSRGGLLNYLREH
                     PELTASGKNNNL"
     gene            complement(1113..1625)
                     /locus_tag="PK146_03725"
     CDS             complement(1113..1625)
                     /locus_tag="PK146_03725"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPE91383.1"
                     /translation="MGKPWITTKGGDKGTTSLGDGTRVPKDHPRVELYGTLDECQAAI
                     GLARASCCYEEIRGELFRIEEAMGSVMGYLALFPGLPEPDPSTLEETVEKVRNIIGET
                     FRFVRPGDSVPGAALHLARTIARRAERIAVTLHRDGEIGEAPFAWINRLSDAIYALSL
                     WTDKTNRECE"
     gene            complement(1690..2124)
                     /locus_tag="PK146_03730"
     CDS             complement(1690..2124)
                     /locus_tag="PK146_03730"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HPE91384.1"
                     /translation="MDVSTIRIRHYAPEGAECGPVQETYETLSNMVEQLGPKLEKMGF
                     PVTLEKVSSGGDPAKNNMVTMESEEIEFPETPLDEILGLEASAKPCNCSAGKGHGDCR
                     TIEIGGDTYQAIPPGLIADGLLRVALSATGGCGSGCGTCGGG"
     gene            complement(2266..2691)
                     /locus_tag="PK146_03735"
     CDS             complement(2266..2691)
                     /locus_tag="PK146_03735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPE91385.1"
                     /translation="MAMEKLEGSRGCFVCDRSGTNPRSLGVTLFWNDDEQQTVIPFTP
                     DDSWCGYEGIVHGGILTALCDDAMAWAAKKALTEWTVTAGMNIRFLRPVRSGHDYTAF
                     GHSTGVEGRKVRTAARIVGEDGKVCVDAKATFVAVKTKA"
     gene            2802..2887
                     /locus_tag="PK146_03740"
     tRNA            2802..2887
                     /locus_tag="PK146_03740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2835..2837,aa:Leu,seq:caa)
     gene            complement(3050..3265)
                     /locus_tag="PK146_03745"
     CDS             complement(3050..3265)
                     /locus_tag="PK146_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPE91386.1"
                     /translation="MAKVPFCTCVDLECPAHPSNHDKGCTPCIAKNLAEECIPVCFYR
                     LVDPEMDRDQDYSFKGFARFLEERRGK"
     gene            3523..4803
                     /locus_tag="PK146_03750"
     CDS             3523..4803
                     /locus_tag="PK146_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPE91387.1"
                     /translation="MLGFLSLIVSGTLLLLVLNAGTRPLGFIDALFTATSAVCVTGLI
                     VVDTGKDLSLASQGVVLFMIQLGGLGVMTATTAFFLLMRERIGLRQRMFFAGGFGLDS
                     PSGVKSLLIRILFITFVIEGIGIIPLFFGFLNSGMTHGSSLYYAAFHSVSAFCNAGFS
                     LFSDNLESFSRTILVPGTVIGLVVIGGLGFVVLNDLREMFRRAEKISLQTKLVLVTTV
                     LLVISGSVVLVLTEWGSAFGDLQPGWKVWNGIFASITPRTAGFDTVAPANFSSAGIFV
                     LVLLMLIGASPGSTGGGFKTTTLAILMVSSWSFLKGRHEVQLWNRRLPFHVVQRSITV
                     VVLYLGTLLVGMGLLALIEPFPFRTLLFEAVSALGTVGLSLGVTPELTYAGKIVLVLM
                     MFWGRVGIVTFLYSLLKREIRERVSYPSEEVPVG"
     gene            4819..5499
                     /locus_tag="PK146_03755"
     CDS             4819..5499
                     /locus_tag="PK146_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPE91388.1"
                     /translation="MTKKDRKNRSKHRVVLVVGLGRFGQALCKSLVEQGNQVIAVDRS
                     RQLVEEMADIVDISVQADATDAEALQKIGAKEADIAVVAIGGNVEASILTTTILKDLE
                     IPEVIARAQNNIHARVLARVGATRVVFPEKDMGERLAAIMMHPWMSHFAQLPGSSFYL
                     GEISPLAEMEGKTLAELDFRTRYNAVVLYIKRSGERFIPKADTVIRNDDRILVAGLKE
                     DIEEWIED"
     regulatory      5690..5808
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
BASE COUNT         1182 a         1541 c         1715 g         1372 t
ORIGIN      
        1 taggaggaag ttactggaac tgctattgag acccccagct tgtggagggc cgagaagtag
       61 aagaagtccc ccattaccag ccagaggacg gagtatacaa agatccttgc catggtcccg
      121 gcggcaaggg gcgcgaaggc cgatgggccc atggcaaaga acaggaagag agcggcgctg
      181 atgatgtagc cggtacacct gagggcgcca agcccggagt aagatattcg ctccacacct
      241 cggcgataga gaatcggtgc tacagcccag gtaacggcgg ccatgaaggc gaggaagtat
      301 cccaaaagtt gtgatgactg catctagaga ttgttgttct tgcctgatgc cgtgagctca
      361 gggtgctcgc ggaggtagtt aagcaggcct cccctggagc gttcgaggtg ggccctcagt
      421 tcggcctcgg ccttgtcggg gtctccggtc tcgatggcgt cgatgatggc cacgtgctca
      481 taggcgaatg tgctcagggg tatgccctcc acggagtagt ggcggcagag gttgatcagg
      541 tccgctatgt tgtggagcat catggtcagg tagcggttgc gcgaaagttc gtagagggat
      601 gcgtggaact tgcggtcttc ggcgaggtag tggctggtct cggtctggtc agtcatggcc
      661 gaaaagacgg ggcgccagta ctccagctcc tcgatgggct ggttcaaggt aacgagcctg
      721 acggctatga tctccatgtg agtcctcagg tcgtagaggt ccgaggcatc cttgatggtc
      781 gggagggaga cgaaggcccc ccggcggggt tccagcgtga cgagtccggt ctgggcaagc
      841 tgcctgatgg cctccctgac aggggtgcgg gataccgcca gcttgtcggc gagatcgacc
      901 tcggagagcc tctctccggg tcttattatc ccctcgacta tggcctcctt gatcttttca
      961 tagacgatct ggcgaaggtc gatattgcgc gcaggcgata gtggatcgtt ctgcatagga
     1021 ccgcctcctg tcgaaggaga aaatgtcttg cgtatactct gtccattata cacctgacat
     1081 cctgtcgcgc aacgtcctgt ttgttaagag attcactcac actccctgtt cgtcttgtcg
     1141 gtccagagag agagggcgta gatagcgtcg gagagccggt tgatccaggc gaagggggct
     1201 tcgccgatct caccatctct gtgaagggtg acagcgatac gttcagccct tcgggctatc
     1261 gtcctggcca ggtggagagc cgcgccgggc acggaatcac cggggcgcac gaaacggaag
     1321 gtttcgccta tgatgttgcg gaccttttcg acggtctctt ccagtgtcga cgggtcgggc
     1381 tcgggaagtc ccgggaagag ggccaggtac cccattacgg agcccatggc ctcctctatg
     1441 cgaaagagct ctccgcggat ctcctcgtaa cagcaggagg ccctggccag gccgatggcg
     1501 gcctggcatt cgtccagggt cccatagagc tctacccggg ggtggtcctt gggcacgcgg
     1561 gtaccgtcgc cgagactggt ggtacctttg tcccctccct tggtggttat ccagggcttt
     1621 cccataagga tcgcctccag aacatatcct gttgaacggg ctgccgacgg cagcctttga
     1681 ctatacccct cagcctccgc cgcaggtgcc gcagccgctt ccgcagccgc cagtagccga
     1741 aagggccacc ctgagaaggc cgtcggctat cagtcccggt gggatcgcct ggtaggtgtc
     1801 gccgccgatc tcgatggtcc ggcagtcacc gtgacccttg cctgcagagc agttgcaggg
     1861 cttcgccgag gcctcgagcc ccaggatctc gtcgaggggg gtctcgggga attcgatctc
     1921 ctcgctctcc atggtaacca tgttgttctt tgccgggtct ccgccggagg agaccttctc
     1981 gagagttacc gggaaaccca tcttctcgag tttcgggccg agttgctcca ccatgttgct
     2041 gagggtctcg taggtctcct gcacggggcc gcattctgct ccttcgggtg cgtagtgcct
     2101 gatccttatc gttgaaacgt ccatgtttat taacctcctt gttggttgtg gatcataaat
     2161 cgaggcgact gactacagcc tcaagcgaca ggaagggcaa gggcatgctt cgttgatcca
     2221 ttccgtcttg cttctatcgc tttttccccg ttcactgttg gccgttcacg cctttgtttt
     2281 tactgccaca aatgttgcct ttgcgtcgac gcataccttg ccgtcttcac cgacgatcct
     2341 tgcggcggtc cttaccttgc gcccttccac tcccgtagag tgaccgaagg cggtgtaatc
     2401 atgccccgat cttacgggtc tcaggaagcg gatgttcatc ccggcggtga cggtccattc
     2461 cgtcagggcc ttctttgctg cccaggccat ggcgtcgtcg cagagcgccg tcagtatacc
     2521 cccgtggacg atgccttcat agccgcacca gctgtcgtcg ggggtgaagg ggatcaccgt
     2581 ctgctgttca tcatcgttcc agaagagagt gaccccgagg gaccgaggat tggtcccgct
     2641 acggtcgcag acgaaacacc ctctggagcc ttccagtttt tccatggcca tggtaatcac
     2701 caccggcggc tattgtaccc taaggggtat gggtagtaca ccccgaaaat gttgccctgt
     2761 ctggacagaa tcctccagct gtggtactac tatcttcgct tgccggagtg gcggaatggc
     2821 agacgcagcg gactcaaaat ccgccgggct ttggcctgtg ggggttcgag tcccccctcc
     2881 ggcaccatat gttaactatc aagggggtcc ccgatctatc ggagaccccc ttgatctcgt
     2941 ttacttctgt catctccagg gcaagggtac ggagacaggt cagggcactt acgggcagag
     3001 ttatcccttg aaaaatggtg tcctgcccgt aagataacga acagcgccgc tatttccctc
     3061 tcctctcctc caggaacctt gcgaacccct tgaaagagta gtcctgatcg cggtccatct
     3121 ccgggtcgac aagccggtag aagcagacgg gtatgcactc ttccgcaagg tttttcgcga
     3181 tgcagggtgt acaccccttg tcgtggttcg aggggtgagc cggacattca agatccacac
     3241 aggtgcagaa aggtactttt gccatgaata accctcccca agatgctggt ccgagtgagg
     3301 caccagtaga tcataccaga gtatttcaac cggctccacg caagagatgc cgaacggtgg
     3361 ctggaacata agcaaaagaa tcgaatggac gcagacctcc acggatcggc atgacaattc
     3421 taccgtggta gaggataatc tttccggtat agatcgttcc ggggaggctg ttgccttata
     3481 agaaccagga catttacctc acttcccata gaaaaaaaga tagtgttagg gttcctttca
     3541 cttattgttt cggggacatt gcttctcctg gtcttgaacg cggggacgcg ccccctgggg
     3601 tttattgatg cccttttcac tgccacctca gcagtatgcg tgacgggact aattgttgta
     3661 gacacgggga aagacctttc gttggcatcc cagggagttg tgctgtttat gatccaactg
     3721 ggcggccttg gtgtcatgac agccaccacg gcctttttcc tccttatgag ggagaggatc
     3781 ggcctccgcc agcgcatgtt ctttgcaggc gggttcgggc tggattcccc ctccggtgtg
     3841 aaaagtcttc ttatccggat actttttata acctttgtga tagaaggtat cggtatcatc
     3901 ccccttttct tcgggttcct gaactcggga atgacccatg ggagttccct ctactatgcg
     3961 gccttccaca gcgtaagcgc tttttgcaat gcagggttct ccctgttctc tgataatctc
     4021 gaaagctttt ccaggaccat actggtacca ggaacagtta ttggcctcgt ggttatcggc
     4081 gggttgggtt ttgtcgtact gaacgacttg agagagatgt tccggcgggc agaaaagatt
     4141 tcccttcaga cgaaacttgt ccttgttacg accgttcttc tcgttatttc tggatctgtg
     4201 gtcctggtct tgacggagtg gggaagcgca tttggagatc ttcagccagg ctggaaggtc
     4261 tggaacggga tcttcgcgag tatcactccc agaacagcgg ggttcgatac ggttgccccg
     4321 gcgaactttt cttcagcagg gatcttcgtg cttgtgctgc taatgctgat tggagcttct
     4381 ccaggctcca cagggggagg tttcaagacg accactcttg cgatcctgat ggtttcgtca
     4441 tggagtttcc tgaaaggacg ccatgaagtc caattatgga acaggaggct gcccttccat
     4501 gtcgttcaaa gatcgatcac ggtggtcgtt ctttaccttg gcacactgct tgtgggtatg
     4561 gggttgctgg ccctgatcga gcccttcccc ttcagaacgc ttctttttga ggctgtttcg
     4621 gctctcggga cggtggggct gtcgcttggg gtgacccccg aattgaccta tgcgggcaag
     4681 atcgtgcttg tcctgatgat gttctggggc agagtcggga tagtgacgtt cctttacagc
     4741 ctattgaagc gcgagatcag ggagagggtg tcctacccgt cggaagaggt tcctgtaggt
     4801 tagaaggggg agaaaaagat gacaaagaaa gaccgtaaaa acaggagcaa acacagagtt
     4861 gtcctggtcg tagggctcgg gaggttcgga caggctctct gcaaatccct cgttgagcaa
     4921 ggaaaccagg ttatcgctgt tgaccggtca agacagcttg ttgaagagat ggcggatatt
     4981 gtcgatattt cggtccaggc tgatgctacc gatgcagaag cacttcagaa gatcggcgca
     5041 aaagaggccg atattgctgt ggttgccatc ggggggaatg ttgaggcaag catccttaca
     5101 acgacgatcc tgaaggacct ggagatacca gaggtcatag cgagggcgca aaacaatata
     5161 cacgcgcgag tccttgccag ggtgggcgct acccgggtag tgttccccga gaaggacatg
     5221 ggggaacgcc ttgcggcgat catgatgcat ccctggatgt cgcattttgc ccagctgccc
     5281 ggttcttcct tttaccttgg tgagataagt ccccttgccg aaatggaagg gaagacgctt
     5341 gccgaacttg acttccggac ccgttacaat gctgtcgtcc tgtacattaa aagatcgggt
     5401 gaaaggttca tccccaaggc agatacagtc atccggaacg acgacaggat tcttgttgca
     5461 ggactcaagg aagatattga agagtggatc gaggattgag accggaccgg tctccaatcc
     5521 cgatatagtc ggaattcttc cccgtgaacg gataatgaaa tgaaaggtac cggaaacatt
     5581 ctttccacgc tggaaatgca tgcaggcaaa agatacgact cccccctttg cccaattccc
     5641 aagggagaag ccagttactt gcgattgatt atcaatagtg ttagcatctg tgtataactc
     5701 cctccgagcc tacctgtcta aggggaatgg acctatgaca ggaggccgaa agggtgtcgc
     5761 tggaaacggc ggcgcctccc gtgtttggga aggaggggcc gctgtcatta
//
LOCUS       DAOPKQ010000075         5713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_15185_length_5713_cov_5.122835, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000075 DAOPKQ010000000
VERSION     DAOPKQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15185_length_5713_cov_5.122835"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PK146_03760"
     CDS             complement(<1..862)
                     /locus_tag="PK146_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91389.1"
                     /translation="MIRLADLSIDLGEFVLDNFSLSVDKGEFFMVVGPSGAGKTVLLE
                     SIAGLRHPSGGRILIGGRDVTGLPPERRRVALVYQDYALFPHMTVEENIRWGLRFLRD
                     PDPGHVEGLFRLLRLERLLERSPDTLSGGEQQRVALARALAVKPSVILLDEPISALDP
                     RFREDLQDYLSRINREGMTIMMVTHDFNEVLSLGHRVAVIVEGSLQQVGDVKTVFREP
                     ANRLVAEFVGMKNLFPSSVKGRRACLEGGVSLVLNGQQRVGECLIGIRPEDVMIGGDL
                     LEDTENVFSGR"
     gene            complement(859..1653)
                     /locus_tag="PK146_03765"
     CDS             complement(859..1653)
                     /locus_tag="PK146_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91390.1"
                     /translation="MDRKFRERWFMLLMGSFLVVYIIWPLAGMFFRADWGLVVSTSRD
                     SVVVGALWRSIWTAAAATGIIAIFGTPLAYFLARQDFKGRSVLEAVIDVPIMVPHTVA
                     GIAVLMAFSPKAPAGAMLVKAGLSPVNSHLGIILACMFVSIPFYVDAARDAFHGVSPR
                     LENVSRTLGASFPHVFFRITLPLAKRGLVSGLIMSWARGVSEFGAIVIIAYHPMVAPT
                     LIYDRFATFGLKYSTPIAVQLILVCLGMFVVLRVLVSGKRRDGAGS"
     regulatory      complement(1712..1832)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2051..2851
                     /locus_tag="PK146_03770"
     CDS             2051..2851
                     /locus_tag="PK146_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPE91391.1"
                     /translation="MRGKFRIREDYVYKMPVHFGGYAFAPVRTVYGDMTTIMMGFETD
                     QEELLKLIPEDFELLQPVVHVQYANCREVDWMSGGEYRLIQLSSPVRYEGNSEGLEGE
                     YPLVIWEDKACPIIGGREEDGMPKVFADIAVERHSGDHWFTSASYECNTFLTLDLDRK
                     EELGTEELARLNENPRINFFGWRYIPRLGKGGASLSHATLYPQEATMIRAWRGDGRVR
                     WHPFTLEMNLVQFSAICSLSNLPVLRYLPALMTKGSARLNVGDSRTLP"
     gene            2871..3677
                     /locus_tag="PK146_03775"
     CDS             2871..3677
                     /locus_tag="PK146_03775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPE91392.1"
                     /translation="MEGYFENKVAVVTGATSGLGLGLTEHLLARGARAVFMGDIKEDN
                     LEKESKRLNQQYPGRIHPLLTDVTKLEQVQGLIEAARSFEGHLDFVFNNAGMGMTLPT
                     EQITFDIWRFIVDLNLMGVVHGTYTAIPIMRKQGSGHIVNTGSVAGLFPVPYQALYAA
                     TKGAIIRMTESLQYELETEGLTFSVFCPGNVRTAIFGDMTPPPDSISVDEAVDYVFGE
                     LEKKEMVIILPENFRQFDALFRQKREEFDRMARQIAAERRENYRTKGTYF"
     gene            complement(3780..4976)
                     /locus_tag="PK146_03780"
     CDS             complement(3780..4976)
                     /locus_tag="PK146_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91393.1"
                     /translation="MEISSVMKRAEIEFDVVVLLVAALVMLVAGTLLLPVSKGALPYY
                     ENGLYGLLLFIFALQMVTLGRTPFGDAPRSRGLMAAGVIIASLGIITCFIPDIFSRVP
                     RIILSICFGPGGAALLLQMIFSRDKLPKWRQYGGIFHHLIAGCSAVYVLSALIGLLVF
                     RQDLISTPMSAMVALMTGLSLFYLATTLQRIYRAYPEAVQEPKGSVDLPIGRAMILLT
                     GIFMVILGVLLVPVSLGRLPFSGSAQLGLLMVILALQMLATGNSPIGPFPRTWLMIII
                     GSLFILLGAVSCIIPGVLVPSLTVLIGVLNILGGGLMLKRIFTPIIGGSGRGGGPVPA
                     ILVRLNLVQVTMNVVSIMFGTSMLVHNLIPGLVVGVILAANGGLLLYLMRIMSVIDGI
                     QKKMER"
     regulatory      5324..5446
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            5516..>5713
                     /locus_tag="PK146_03785"
     CDS             5516..>5713
                     /locus_tag="PK146_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91394.1"
                     /translation="MMILSCVIADSSLELQSQYAVALSRTQNLRLKETVTTGGELEDC
                     LKRANAHLVLLDIFLNGWGGLS"
BASE COUNT         1397 a         1622 c         1577 g         1117 t
ORIGIN      
        1 cccggccgga gaagacattc tccgtatcct ccaaaagatc gccccctatc atcacgtcct
       61 ccggacgtat cccgatcagg cactccccta ccctctgctg tccgttcagg acaagggaca
      121 ctcccccttc caggcacgcc cttctgccct tcacgctgga agggaagaga ttcttcattc
      181 cgacgaactc cgccacgagc ctgttagccg gttcacggaa gacggtcttg acgtcgccca
      241 cctgctgaag ggacccttcg actatcacgg cgaccctgtg tccaagggag aggacctcgt
      301 tgaaatcgtg ggtcaccatc atgatggtca taccctcccg gtttatccgg gagaggtagt
      361 cctgcagatc ttccctgaac ctgggatcaa gggccgatat cggttcatcg aggaggatca
      421 ccgatggctt taccgcaagg gccctggcca atgccacacg ctgctgttcc cctccggaaa
      481 gggtatccgg ggaccgttca agcaggcgct caagtcttag cagcctgaag agcccctcca
      541 catggccggg gtctggatcc ctgaggaatc tcaggcccca ccggatgttc tcctcaacag
      601 tcatgtgcgg gaacagggca tagtcctggt agacaagggc tacccttctt ctctcgggag
      661 ggagccctgt gacatcgcga cccccgataa ggatccttcc tcccgaggga tgcctcagtc
      721 ctgctatcga ctccagaaga accgtcttcc ccgcgccgct ggggccaacc accataaaga
      781 actctccctt gtcaacggag agggaaaagt tatcgagtac gaactcgccg agatcaattg
      841 aaaggtcggc cagtcggatc aacttccggc accatccctg cgtttcccgg aaaccaggac
      901 cctgagaacg acgaacatac ccagacagac caggatcagc tgcaccgcaa taggcgttga
      961 gtacttcagg ccgaaagtgg cgaaccggtc gtagatcagc gtgggggcca ccatcgggtg
     1021 gtaggcgatg atcacgatgg cgccgaactc gctgaccccc ctggcccagc tcatgatcag
     1081 gccagagacg agccctctct tcgcgagtgg caaggtgatc cggaagaata catggggaaa
     1141 ggaggctcca agggtgcgtg agacattctc aagtcttggc gacactccgt ggaaggcatc
     1201 gcgggccgca tcgacgtaga aaggtatcga gacgaacatg caggccagga ttatccccag
     1261 atggctgttg accggggaaa gtccggcctt cacaagcatc gcgccggccg gggccttggg
     1321 agagaaggcc atcaggacgg ctatgccagc gacagtatgg ggtaccatta tcgggacatc
     1381 gatcaccgct tcaagcaccg acctgccttt gaagtcctga cgggccagga agtaggccag
     1441 gggtgtcccg aagatggcga tgatccccgt tgcagctgca gcggtccata tgcttcgcca
     1501 aagggccccg acgaccacgc tgtcccgtga ggtggacacc accagacccc agtcggccct
     1561 gaagaacatc cccgcaaggg gccatatgat atagaccacc aggaaagagc ccatgagtag
     1621 catgaaccag cgttccctga acttgcgatc caaagagata cccccagaca aaaaagaggc
     1681 cgccccccaa agaaccggag gcggcctcta atatcggctg accgctcctc ctttccaatc
     1741 aagggaggcc ttcccgtttc cgggaaaacc cgtcggtcgc tccccgtgga tcgagatccc
     1801 ttaggggata cggctcggag gatgcccaag tatcaggcat taagaatgct aacacaacca
     1861 cacagtgatg gcaatgataa tccttatcgt tagagccggg taactccctc ctgagataca
     1921 gccaggtatc aggtctttgc ctgacgatca gggaggcatg ataacgaatg gacaaccagg
     1981 ggggtgagca ttattatgaa attgttcatt tagaaactca attaaacaaa ccacgcaagg
     2041 aggtggcctt ttgagaggaa agttcaggat cagggaggac tacgtctaca agatgccggt
     2101 acatttcggg ggttatgcct tcgctccggt aaggacggtc tacggggata tgaccaccat
     2161 aatgatgggc ttcgagacgg accaggaaga actgctgaag cttattcccg aagattttga
     2221 actgcttcag cctgtcgttc acgtacagta cgcgaactgc cgcgaggtgg actggatgtc
     2281 aggcggagag tacaggctta tccagctctc ctcccccgtc aggtacgagg ggaactccga
     2341 gggactggag ggagagtacc cactggtaat ctgggaggac aaggcctgtc ccatcattgg
     2401 aggccgcgag gaggacggga tgcccaaggt tttcgccgat atcgccgtcg agcgccattc
     2461 aggcgatcac tggtttacct ctgccagtta cgagtgcaac acattcctca ccctcgatct
     2521 tgacaggaaa gaggaattgg gaacagaaga gctcgccaga ttgaacgaga atcccaggat
     2581 caactttttc ggatggcgct acatcccccg gctcgggaag ggcggagctt ccctgagtca
     2641 tgcgacgctc tatccccagg aagcaacaat gatacgggcg tggagaggcg atggaagggt
     2701 cagatggcat ccatttacgc ttgagatgaa ccttgtccag ttcagtgcga tctgttccct
     2761 gtcgaatctt cccgttctaa ggtatctgcc cgccctgatg acaaagggtt cggcaaggct
     2821 caacgtcggg gattcaagaa cccttcccta gggtccaaag gaggccggga atggaaggat
     2881 acttcgagaa caaggttgcc gttgttaccg gtgcgacatc aggattgggc ctgggcttga
     2941 cggagcacct tctggcaagg ggcgcccggg ctgttttcat gggcgatatc aaggaggaca
     3001 acctcgagaa ggagtctaaa aggctgaacc agcagtatcc gggcaggatt catccattgt
     3061 tgaccgatgt cactaaactg gaacaggtcc agggactgat cgaggcggcc agatcattcg
     3121 agggccatct cgacttcgtc ttcaacaatg ccggtatggg catgaccctc cccacggagc
     3181 agatcacttt cgacatctgg aggttcatcg tggacctgaa cctcatgggc gtcgtccacg
     3241 gcacttacac cgccataccg ataatgcgca aacaggggtc cggccatatc gtgaacacgg
     3301 ggtccgtggc aggcctgttc cccgtcccct accaggcctt gtacgctgcc acaaaagggg
     3361 cgatcataag gatgacggag agtcttcagt acgaactgga aacggagggt ctcaccttca
     3421 gcgtcttctg ccccgggaac gtaaggacgg ccatattcgg tgacatgaca cccccgcccg
     3481 attccatcag cgtcgacgag gcggtggact acgtcttcgg ggaactggag aagaaagaga
     3541 tggttataat ccttcccgaa aacttccggc aattcgatgc ccttttccgg caaaagagag
     3601 aggaattcga caggatggcc cggcagatcg ccgctgagcg acgggagaac taccgaacaa
     3661 aggggacata tttctaggat ctgcgtctcc cttcccgaac aagatacttg acccaagaaa
     3721 gagggggccc cggaaggggc cccctgacac gatcacgctt cagaagacct tcagaccgcc
     3781 tatcgctcca tctttttctg tatcccgtcg atcacggaca ttatgcgcat caggtacagg
     3841 agaagcccgc cgttcgcggc aagtatcact cctaccacca ggccgggtat gaggttatgg
     3901 accagcatgg aagtcccgaa catgatggag acgacattca tcgtcacctg gaccaggttg
     3961 agtctgacca gtatcgccgg gacaggacca cctccccggc cggaaccccc gatgatggga
     4021 gtgaagatcc tctttagcat caaccctccc ccgagaatgt tcaaaactcc gataaggacc
     4081 gtcagcgagg gtacgaggac cccgggaata atgcaagaca ccgctcccag caagatgaaa
     4141 agagatccga tgatgatcat cagccacgta cgggggaagg gacctatggg agaatttcct
     4201 gtagcgagca tctgcagcgc caaaatcacc atcagcaggc ccagctgcgc gcttccggaa
     4261 aaagggagcc tccccaggct cacaggcacg agcaagactc cgaggatgac catgaagatt
     4321 cccgtcagaa ggatcatcgc cctcccgatg ggaagatcca cacttccttt tggttcctgg
     4381 accgcctcag ggtaggcccg atagatcctc tggagggtcg tagccagata gaaaagggat
     4441 agtccggtca tgagcgccac catggcgctc ataggggtag agataaggtc ctgtctgaaa
     4501 acaagaagcc cgataagggc agaaaggaca tagaccgccg agcaacctgc tatgagatga
     4561 tggaagatgc cgccgtactg cctccattta ggaagcttgt ccctggaaaa tatcatctgc
     4621 agcagaaggg ccgctcctcc cggaccgaag cagatggaca ggatgatcct ggggacccgg
     4681 ctgaaaatat cggggataaa gcaggtgatt atgcccaggg aggcaattat cactcccgcc
     4741 gccataagtc ccctggacct gggtgcatcg ccgaagggcg tccttcccag ggtgaccatc
     4801 tgcagggcaa agatgaaaag aagtagcccg taaagaccgt tctcgtagta cggtagagcg
     4861 cctttcgaga cgggaaggag aagggtacct gcaaccagca tcaccagggc tgcgacaagg
     4921 agaacgacca catcgaactc gatctcggct ctcttcatga ccgaactgat ctccatgaca
     4981 tcatcccctt cgatcgtatg acctactgaa aagggatcct atttcagaaa ttccccggcc
     5041 aggagagtaa atcggcagcc cccgggttct tgttcccata aaactggacc tagtgatgca
     5101 actatcatga ttcaacatca tcatcttcga tacccgattc cggaacatct cccttgccat
     5161 cctgacttca cgacaccctt tatttggaga gcaaggcaag atacggtctt cagagaccgt
     5221 ctttataata aacaatttcg ttctctgttg ttcatttttg gccatccaaa aggggagtac
     5281 ggtaaaaaaa cttccttacc tggactctgt gggttataat atcaaaaaag cgtttctccg
     5341 agccggcccg cctaaaggca ttaccccccc caagggagcc ggacgacagg gtttagctgg
     5401 aaacggctac gcctcccttt tggaaaggag gaacaggacc attgggtagt gcactactag
     5461 tgtgctttga cggtttgttg ctgattcacc cttccatcgc caggggggtc actccatgat
     5521 gatcctttcc tgtgtgatcg ccgacagttc gctggagctc cagagccagt acgcggtggc
     5581 tctttcaaga acccagaatc tccgtcttaa ggagaccgtc accacaggag gggagcttga
     5641 ggattgcctt aaacgcgcca atgcccacct ggtcctcctg gacatcttcc tcaatgggtg
     5701 gggtggcctt tcc
//
LOCUS       DAOPKQ010000076         5699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_15228_length_5699_cov_5.069454, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000076 DAOPKQ010000000
VERSION     DAOPKQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15228_length_5699_cov_5.069454"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /gene="glgP"
                     /locus_tag="PK146_03790"
     CDS             <1..1215
                     /gene="glgP"
                     /locus_tag="PK146_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPE91395.1"
                     /translation="LRMLRDLGYSNIDDYHLNEGHAAFLALELIREMGYENYDRVREK
                     GIFTTHTPVSAGHDHFSWDLINRVMDRSMAARLRRMMPTEDVSMTEIALRYSRFINGV
                     SEKHTKVSRAMYGREDVDCITNGIHTLTWVSPDLAEVFTKYIPGWDNAPERLVKAVQI
                     PVEEIRSAHAPAKKRLLDYVEEKTGKKLDPERLTIGFARRVAQYKRADLVLRDTSRLV
                     KAAGGKVQFIFSGKAHPNDNPAREILRKLLCEAQNMVGIDIPVVFIEDYDMDKAALLV
                     QGVDLWLNNPVRPREASGTSGMKCALNGIPNFSVLDGWWVEGCVEGVTGWSIGPEPKE
                     SISDQYDDSVDLDDLLEKLEKVIIPTFYERPREWGEIMRGAIALNASYFNTHRVVREY
                     CEKAYGIQMRGL"
     gene            complement(1277..1465)
                     /locus_tag="PK146_03795"
     CDS             complement(1277..1465)
                     /locus_tag="PK146_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HPE91396.1"
                     /translation="MPIVRVDLWSGRSQEFKAELAKAITDVVVQKVGCPVEAVTVKIE
                     ESPVENWMIGGKQCEKKA"
     gene            1673..2950
                     /locus_tag="PK146_03800"
     CDS             1673..2950
                     /locus_tag="PK146_03800"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPE91397.1"
                     /translation="MLNRDSQDLASEWILLNQEVMSLHEKGQYERAASVALRTLELAD
                     RFGDPDHPYLAASLNNLAGIYDAQGRYLEAEPLYQRSLEIRERVKGSGHADVAATLNN
                     LAELYRAQGRYFEAEPLYRRSIRILENVMGPDHPDVATCMNNLAGLCDARGNYEEAER
                     LFRKAFEIRSRSLGKGHPDVAVTLNNIAALYENQGWFTEARPLYRRALSILRKALGSD
                     HPDVATCINNLAGFYRTQGKYAKAEKLYKRSHDIRKKSLGANHPYVAASLTNIAELYF
                     AQGKHSQAESLFKRSIAILERALGPDHPDVATGINNLAGLYDTLGKHEMALPLCRRAL
                     AIRTRSLGRWHPHVAVSLNNLAGLYESRGMLQQADPLYGRSLQILEKTLGPAHPDVAV
                     CLDNMADLMRRMGRDEEASRMEQNARKIRGEEE"
     gene            3083..3472
                     /locus_tag="PK146_03805"
     CDS             3083..3472
                     /locus_tag="PK146_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91398.1"
                     /translation="MDQRSSEMKVVYELEYDEEKKIITGRITGELNRAVAAAMASRLA
                     ELSLRHGCFRLLNDLREADLIPSVLDIYMIPREVEKAGILKTFKRALLVRPPLDDFRF
                     LETVSFNEGHRLRVFRDPEEALQWLLE"
     gene            3575..3790
                     /locus_tag="PK146_03810"
     CDS             3575..3790
                     /locus_tag="PK146_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPE91399.1"
                     /translation="MSGVYKVIELVGCSETSWEDAACCAVETASKSLRDLRVAEVVMQ
                     DVTIKDNKIDQYRVKLKVSFKYHCEED"
     gene            complement(3855..4322)
                     /locus_tag="PK146_03815"
     CDS             complement(3855..4322)
                     /locus_tag="PK146_03815"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPE91400.1"
                     /translation="MLSVRETVIGPVGIEEDEGSITRLFLPNKARRIKGPDGEGTPLL
                     AEAFGQLDLYLEGKLQRFSLPLRPEGTEFMKRVWRELLKVPYGRTTTYRELAIAAGSP
                     GGSRAAGMANAKNPIAIFIPCHRVIGSNGKLVGFGGGLELKSWLLDLEARNME"
     gene            complement(4371..5201)
                     /locus_tag="PK146_03820"
     CDS             complement(4371..5201)
                     /locus_tag="PK146_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPE91401.1"
                     /translation="MEKDIIRDPLCIDETYPPDMHSLFIQERLLGLIYLADGEGPHKT
                     LLLLHGFPGNEKNLDIAHMIRRGGYNVLVFHYSGSWGSRGSYSFSQNYSDLQAVRSIM
                     ADAAFAREHRIDRDNIYLAGHSVGGFLTLLAARDGLDFKGFAPLAPYDLGKQARRIRN
                     EDKEAFRETLELFRGGMAPLSGATPESLMEDILDNHDRWDLLGAPSAYSERRMLLVMA
                     AEDRCAWPKIHQIPVAETLARAGGDKTVISLSCSHDFPDKRVSVAEAIFEWLEEKEER
                     "
     gene            complement(5242..>5699)
                     /locus_tag="PK146_03825"
     CDS             complement(5242..>5699)
                     /locus_tag="PK146_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPE91402.1"
                     /translation="ERLSGEEIWRDTEGRVDAFVDMAGSGGSFTGISSVLKEHDPRIR
                     CYLGEPAEAAFFGNPGGSGRHRIQGCGYSRELPLLDRSLVDGFVSMNDNEAIEGARAL
                     ARFEGIFGGFSSGAHAAAARRLLEGPEKGKTVVFLVCDSGLKYLSTDLY"
BASE COUNT         1310 a         1604 c         1588 g         1197 t
ORIGIN      
        1 ctgaggatgc tccgcgacct gggctactcc aacatcgacg actatcacct caacgagggg
       61 catgccgcgt tcctcgcact ggagctaatc cgggagatgg gttacgagaa ctacgacagg
      121 gtaagagaga aaggtatatt caccacccat accccggtgt cagcggggca cgaccacttc
      181 agctgggacc ttataaaccg tgttatggac agatccatgg ctgcccggct ccgcaggatg
      241 atgcccaccg aggatgtctc catgacggag atagcactcc ggtacagccg ttttataaac
      301 ggtgtctcgg aaaaacatac taaggtaagc agggccatgt acggaagaga agacgtggac
      361 tgcattacca acggcataca caccctgacc tgggtgagcc cggatttggc agaggtcttc
      421 acgaaatata tccccggatg ggacaacgca ccagaacggc ttgtcaaggc tgtgcagata
      481 cccgtcgagg agatacgctc cgcccatgcg ccggccaaga aaaggttgtt ggactacgtg
      541 gaagagaaga caggaaagaa acttgatcct gaaaggctga ccatcgggtt tgcaagaagg
      601 gtggcccagt acaagagagc cgacctggtt cttcgcgata cgtccaggct ggtcaaggcc
      661 gcagggggta aggtgcagtt catcttctca ggcaaggctc accccaatga caaccccgcc
      721 agggagatcc tcaggaagct cctctgcgaa gcccagaaca tggtcgggat cgacatcccg
      781 gtggtcttca ttgaggacta cgacatggac aaggcagccc tgctcgtaca gggtgttgat
      841 ctctggctca acaaccccgt ccgccccaga gaagcatcag ggaccagcgg aatgaagtgt
      901 gctcttaacg gcattcccaa cttctccgta ctcgacggct ggtgggtcga ggggtgcgta
      961 gagggagtca caggttggag cataggcccc gaacctaaag agagcatcag cgaccagtac
     1021 gatgattccg ttgacctcga cgacctcctt gagaagcttg agaaggtgat cattcccaca
     1081 ttctacgaaa ggccccggga gtggggcgag atcatgcgcg gggccattgc ccttaatgcc
     1141 agctacttca acacccaccg cgtggtccgt gagtactgtg aaaaggccta cggcatacag
     1201 atgagagggt tataaaaaga tgaagtgtga agggtgaagg gtgaagggtg aagtgaaagg
     1261 ggacaaactt cacccttcag gccttctttt cgcactgctt tccgccgatc atccagttct
     1321 caacgggcga ctcctcgatc ttgacggtta ccgcctcaac agggcatccg accttctgaa
     1381 ccactacgtc ggtaatggcc tttgccagtt ccgccttgaa ttcctgactt cttccggacc
     1441 agaggtctac tctcacgata ggcacgcttt cacccccctt ctccgatcat tttccgtata
     1501 tttgtgctta aaaggggccg gtaatttgaa atgtatcttg aattataaca attaaactat
     1561 tactatagta tctgaaaaaa ccgttggagc caaagatctg gccagacgtc gggcatggaa
     1621 cgcagagagg taatgtctat agggcgttgc caagtaacgg gggaattttt ccttgctgaa
     1681 ccgggactct caggatcttg cttccgaatg gatccttctt aaccaggaag tcatgtccct
     1741 tcatgaaaag ggacagtatg aacgagcggc atcagtagct ctcaggactc ttgaactggc
     1801 cgatcggttc ggggaccctg accaccccta ccttgcggcc agcctgaaca acctcgccgg
     1861 gatctacgac gcccaggggc gctacctaga ggctgagccc ctataccaga gatcacttga
     1921 gatacgggag agggtcaagg gctcgggaca cgctgatgtg gctgccacac tgaacaatct
     1981 ggctgaactc tatcgtgccc aggggcgtta cttcgaggct gaacccctct acagaaggtc
     2041 cataaggatc ctggagaatg taatgggccc ggaccatcct gacgtggcca cctgcatgaa
     2101 caatcttgcc gggctctgcg atgccagggg taattacgaa gaggccgaga ggctcttccg
     2161 aaaagctttc gagatcagga gcaggtcgct gggcaaaggg caccccgacg tggccgtaac
     2221 tctcaacaac attgccgcac tttacgagaa ccagggttgg ttcaccgagg caaggccttt
     2281 gtacaggcga gccctgtcca tcctccggaa agctcttggt tccgaccacc ccgacgtggc
     2341 cacctgcata aacaacctcg ctggttttta ccggacccag gggaagtatg ccaaggcaga
     2401 gaagctctac aagcgatccc acgacatccg caagaagtcc ctgggagcaa atcaccccta
     2461 tgtggccgca agcctgacca acatcgcgga actgtacttt gcccagggca agcactcaca
     2521 ggctgaaagc ctcttcaaaa ggtcgatcgc catacttgaa agagcccttg gtcccgatca
     2581 ccctgatgtg gctacgggca tcaacaacct ggcagggctt tacgacaccc tcgggaagca
     2641 tgaaatggct ctccccctgt gccgaagagc tcttgccata aggaccaggt cgttggggcg
     2701 ctggcacccc cacgtggcgg taagtctcaa caacctggcc ggcctctatg aatccagggg
     2761 catgttacaa caggcagatc cactttacgg gcgctccctg cagatactgg aaaagaccct
     2821 cggcccggcc catccggatg tggcggtgtg cctcgacaat atggccgacc ttatgcggag
     2881 aatgggccgc gacgaggaag cctcccgaat ggagcagaat gccaggaaga tcagggggga
     2941 agaggagtag ttcgagttca agatcctcac agatcgacaa ttccggaagc gggaaaggga
     3001 ggatccggga cgttaggttg ggaaaatccc ggcctacaga aaaaaccttg atccacctgt
     3061 tccggatcgg cgatccggcc agatggatca gagatcgtcg gagatgaaag tggtatacga
     3121 actggaatac gacgaagaga aaaagatcat tacagggcga ataacaggag agcttaacag
     3181 agctgtcgca gcagcaatgg catcacgact tgctgaactg agtcttcgtc atggatgctt
     3241 ccggttgctc aacgacctca gggaagcgga tctgatcccg tcggtactgg acatatacat
     3301 gatcccgcga gaggtcgaaa aggccgggat cctgaagacc ttcaaacgag ccctgttggt
     3361 aagaccgcca ctggatgatt tcaggttcct tgagacagtc tccttcaacg agggacaccg
     3421 gttgagagta ttcagggacc ctgaagaagc cctccagtgg ctgttggaat agatcagggg
     3481 aagttgaatt gcaatgctat tgtcgcagcg atatactatc actatgaaat aataaccagg
     3541 catctcattc gatccagtga aagggggcgt ggctgtgagc ggtgtctata aggtcatcga
     3601 actcgtggga tgcagtgaga cctcctggga ggatgctgcg tgctgtgccg tcgagaccgc
     3661 ctcgaagagc ctcagggatc tccgtgtagc cgaggtggta atgcaggacg tcaccatcaa
     3721 ggacaacaag atcgaccagt acagggtcaa gctgaaggtg tccttcaagt accactgtga
     3781 ggaagattag cggaagcatc aagatcacag gggggaggca ttgccgcccc ccctttctga
     3841 tttccgggaa cgggtcactc catgttcctg gcctccaggt ccaaaagcca tgatttgagc
     3901 tccaggcctc cgccgaagcc gaccagtttc ccgttacttc cgatgaccct gtgacagggg
     3961 atgaaaatgg cgatggggtt cttggcgttg gccattcctg cagccctgga ccctccggga
     4021 cttccggcag cgatggccag ttccctgtag gtggtcgtcc tgccgtaggg gaccttcaac
     4081 agttccctcc agaccctttt catgaattca gttccttcgg gcctcagggg cagcgagaac
     4141 cgttgcaact tcccctcgag atagaggtca agctggccga aggcttcagc caaaagaggg
     4201 gttccctctc catcgggtcc cttgatcctt ctcgccttgt tgggcaggaa aagccttgtg
     4261 atggaaccct catcttcctc gatacccact ggccctataa ctgtttccct tacgctgagc
     4321 atcagccttc ctccctggga tcgaaacttg aaatagcaag cggtcagcgt ttacctctcc
     4381 tctttttctt caagccattc gaatatcgcc tcagccacgg atactctctt gtcgggaaag
     4441 tcgtgggagc aggagaggga tatcaccgtc ttgtcgcctc cggcccttgc cagtgtctcc
     4501 gccaccggga tctggtgtat cttaggccag gcacagcgat cctcagcggc catcacgagg
     4561 agcatccttc tttccgagta ggcggagggg gcgccgagaa gatcccatct gtcgtggttg
     4621 tcgaggatat cctccatgag agactcaggc gtcgctccgc tgaggggtgc catccctccc
     4681 cggaagagct ccagagtctc cctgaaggcc tccttgtctt catttctgat cctcctggcc
     4741 tgtttcccga gatcgtatgg cgccagggga gcgaagccct tgaagtccag cccgtccctt
     4801 gctgcgagga gagtcagaaa gccgccaaca ctatgtccgg caaggtagat gttgtccctg
     4861 tcgatcctgt gctcccttgc gaaggccgcg tcggccatga tgctcctgac ggcctggagg
     4921 tcactgtagt tctggctgaa ggagtagctt cccctgcttc cccatgatcc ggagtagtgg
     4981 aagaccagca cgttgtaacc acctctccgt atcatgtggg caatgtcgag gttcttctcg
     5041 ttgccgggga agccgtgaag aagcagcaga gttttgtgtg ggccctcgcc gtcggccagg
     5101 tagataagtc caaggagccg ttcttgtatg aagaggctgt gcatgtcggg ggggtatgtc
     5161 tcatcgatgc aaagggggtc tcggattata tccttctcca tctctaccat ctcctctatg
     5221 tatcatcaat atccttttcg ttcagtagag atcggtgctc aggtatttga gccccgaatc
     5281 gcataccaaa aataccactg tctttccctt ctctggcccc tccagtagcc tacgggccgc
     5341 cgcggcgtgt gcacctgatg agaagccccc gaagatccct tcaaaacgcg ccagagcccg
     5401 ggctccctcg atggcctcgt tgtcgttcat ggatacgaag ccgtccacca gggatcggtc
     5461 cagcaggggc agctccctgg agtagccgca tccctgtatc cggtgcctgc cgcttcctcc
     5521 cgggttcccg aaaaatgcgg cctcagccgg ttcgccgagg tagcatctga ttcgggggtc
     5581 gtgctccttc agtacgctcg agatccccgt gaaggagccc cccgatcctg ccatatcaac
     5641 gaaggcgtcc accctgcctt ccgtgtcgcg ccatatctcc tcaccggaga gccgttcac
//
LOCUS       DAOPKQ010000077         5670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_15320_length_5670_cov_3.952627, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000077 DAOPKQ010000000
VERSION     DAOPKQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15320_length_5670_cov_3.952627"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PK146_03830"
     CDS             <1..766
                     /locus_tag="PK146_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPE91403.1"
                     /translation="TFWFYVDFNQSGEIFDQARVWGEKLGPYVGDASLTIFEALVNGN
                     NVLFEGAQGTLLDLDHGTYPFVTSSSPVAGGACVGAGLGPSLVGRVTGVVKAYCTRVG
                     EGPFPTEHRGKSGDVLRERGGEFGATTGRPRRCGWLDLVALKYAVRVNGIHALALTKL
                     DVLSGFETIPVCVSYRSDEGENLPFKGNLTFLDSVEPVFRNMDGWKRDLGECRSFDDL
                     PRAARDYIRFIEEETGVPVVLVGVGPSRDQTIVRGL"
     gene            complement(996..1070)
                     /locus_tag="PK146_03835"
     tRNA            complement(996..1070)
                     /locus_tag="PK146_03835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Val,seq:cac)
     gene            1341..1880
                     /locus_tag="PK146_03840"
     CDS             1341..1880
                     /locus_tag="PK146_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91404.1"
                     /translation="MEAILSDRKLLDIFWSEFGFEEWSGLGLKGVYRRVTFRKDSLMG
                     EVARYYSDDYILSAAGGYSMGRELLEVWKPGKDVMSHRVLLVGNATWGSPLHKDFLMG
                     FSGWVEVMCYRPGDPHSVRKFSDLSTLVNNAGVVLSRLEEGLDPMRVRVPDPGRRGVA
                     AGEPRNPAPFEILKKLFRR"
     gene            1899..3500
                     /locus_tag="PK146_03845"
     CDS             1899..3500
                     /locus_tag="PK146_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPE91405.1"
                     /translation="MFLVAAVLFVAAYFTYGNFQARVYGLSNENKPPSEVYFDGVDYV
                     PAHPSVLLGHHFASIAGAGPIVGPITAAAIFGWLPTYLWCVIGSIFFGGVHDMGALVS
                     SMRHKGLSVGEVVDRWIGRRGKLLFLTFTWLALVLVVAVFLELSAGSFASDPAVAFSA
                     TLYIFMALVFGVMVYKKGIPLSVSTLIMVPIVIGAVFFGSYSDWVQANFNFDINTWRM
                     ILAVYIFLASVLPVWLLLQPRDYLASYMLYFSVLIGAIGMIFGSKFATDFPAFKGFYG
                     GGTNYMWPMLFVIVACGAISGFHSLVGSGTTSKQLKTEKDAQLVGYGSMLLEGQVAVI
                     AIGTVVVAGGIMQGGPTITYGHGIGQFASLIGIDPKVGMSLGLLALNSFLLTSLDTAT
                     RLARYQLQEFFDMKLNKYVATLIGVLGALFLLYYKTGGKPAWSLIWPIFGASNQLVAA
                     LALLAVAVWVKNGLKKENKFLMYPFFFMIATTIAALFLLIKSNLNNMLLAGLSVVLIV
                     LAVMLLKEARDALMGKGPKLEDTAK"
     gene            complement(3597..3770)
                     /locus_tag="PK146_03850"
     CDS             complement(3597..3770)
                     /locus_tag="PK146_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypA/HybF"
                     /protein_id="HPE91406.1"
                     /translation="MRKYRCLHCRHEFEVEDLSPGLKCPKCLNRFVQLVEGDPVKGKS
                     WGSKSFSVPKRGD"
     gene            complement(3893..4573)
                     /locus_tag="PK146_03855"
     CDS             complement(3893..4573)
                     /locus_tag="PK146_03855"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPE91407.1"
                     /translation="MSVLTVLSALWLGVLASISPCPLAANMAAFSFIQKEGLSPRRVL
                     VSGCLYSAGRSVAFAIAGVIVISGLLAIPEASIFMQRHMNRIIGPILLIVGIFVLDLL
                     EIRRRRPIVDSVSSGAPFRGMAGSFFMGVLFALAMCPVSAALFFGGLIPLATEVGSRV
                     FFPALFGLGSGLPVTILSVIFSMGTRAVSRSIGTVYNLQKVIQAASGVLLILLGTYFS
                     LTSTFGLL"
     gene            complement(4570..5061)
                     /locus_tag="PK146_03860"
     CDS             complement(4570..5061)
                     /locus_tag="PK146_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91408.1"
                     /translation="MNYKKTAGYLILLLALAGLVNAGLDIYGDIATGGRGDRGTVTAS
                     GSGYPEGPLELVIYFFEGNAPCSVCERIRGMTYELFESDPEDLSLFSIRDVNVEEKGN
                     ERYVLELDLFSTSVVLAEESGGRIFRWKNLAGVWDLAGDSEAFRDYMLREIEAFRSGG
                     ENR"
     gene            5060..>5670
                     /locus_tag="PK146_03865"
     CDS             5060..>5670
                     /locus_tag="PK146_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPE91409.1"
                     /translation="MTPPDLIGVMMGTCSKYTIKGILVSSFEVNAVDRRSLQMVKNGN
                     NDKEKTALRITGMTCAACSGAVERSLRKVEGVDFAAVNLATETALVVHGRDVSLDELK
                     KAVERVGYGVSDELPEEVDRIRYLRSRRDLLQALVVTVPLMVLMLLHMTGSHLPWFPL
                     VELAAAGFVVFYVGRGSIRGAWIAVTHFHTNMDHYDPICSGHVL"
BASE COUNT         1227 a         1557 c         1596 g         1290 t
ORIGIN      
        1 cactttttgg ttttacgtcg atttcaacca atccggtgag atcttcgatc aggccagggt
       61 ctggggggag aagctcggac cctatgtcgg cgatgcctcc ctgacgatct tcgaggccct
      121 ggtgaatgga aacaacgtcc tcttcgaggg ggcccagggt acacttcttg acctggatca
      181 cggtacctac ccctttgtta ccagttcaag ccctgtggcg ggtggggctt gcgtgggagc
      241 aggtcttggt ccttcccttg ttgggagggt caccggtgtt gttaaggcct actgtaccag
      301 ggtgggtgaa ggccctttcc ccactgagca caggggtaag tccggagatg ttcttcgcga
      361 aaggggcggt gaattcggag ccacgacggg tcgccccagg cgctgcggat ggcttgacct
      421 ggtggccctg aaatatgccg tcagggtcaa cgggatccat gccctggcac tgaccaagct
      481 ggatgtcctc tcggggttcg agaccatccc tgtctgtgta agctacaggt cagacgaggg
      541 ggagaacctt cccttcaagg gcaacttgac ctttctcgac tccgtcgaac cggtgttcag
      601 gaacatggac ggatggaaga gagatcttgg ggagtgccgg agtttcgacg atctgcctcg
      661 ggccgcaagg gactacatcc gctttatcga agaggagacc ggtgttcctg tggtgttggt
      721 aggagttggc cccagcaggg accagaccat agtgaggggc ctctgataat tcttcagcgc
      781 atggccgaaa aggagaggac cgcttccagg gatagaactg gggcggtcct gctttttggg
      841 ttccagggta cggaaaagag agaaacccac accccatagg aaaaactccc ttccttacat
      901 ttcaagagag aaagcaaaaa taccaacgga aaaaacctgc tctgtgaact cttccctgac
      961 ggaagaacga agtgaaagaa ggggataata tccagtggtg ggcgacacag ggttcgaacc
     1021 tgtgacctct tccgtgtgag ggaagcgctc ttccgctgag ctagtcgccc ctgaaagcca
     1081 cgggtcatta tatccggaag gcggtccggt ttgcaagggg cgctgggaac gcgcgacatc
     1141 ctaaaggtca tttgacctta catggctcaa atagtaggat aacctggata aacgggaaaa
     1201 gtgctatttc tctaagcagg gaatgacctg accctccgcg gagtccgggg ctgccggttt
     1261 tttctttggg aaaaccttaa aaaacgattg ccaactggat atggtattac gtataatgta
     1321 tccgaacgga tggtgatatc ttggaagcta ttctttccga caggaagctt ttggacatct
     1381 tctggagcga attcggtttc gaagagtggt ccggtctggg gctgaaggga gtctacagaa
     1441 gggttacctt cagaaaggat tcccttatgg gagaggtcgc ccgctactac tcggacgact
     1501 acatcctttc ggctgctgga ggttacagta tgggccggga gttgctggag gtatggaaac
     1561 ccgggaaaga tgtcatgtcc caccgtgtac tgcttgtcgg gaatgccacc tgggggagcc
     1621 ccttgcacaa ggatttcctt atggggttca gcggatgggt ggaggtcatg tgctatcgcc
     1681 ccggcgatcc ccactctgtc cgcaagttct cggacctctc aaccctcgtc aacaacgccg
     1741 gtgtagtgct gtcaaggctg gaggaaggtc tggacccgat gagggtgagg gtccccgatc
     1801 cgggaagaag gggggtggct gctggagagc ctcgcaatcc ggctcctttt gagattctga
     1861 aaaaactctt tcggaggtga aaaaccgtgc ttgcgatcat gtttctagta gcagcagtct
     1921 tatttgtcgc agcttatttt acttacggca acttccaggc ccgggtttac gggctgagca
     1981 acgagaacaa acctccttcg gaggtctatt ttgacggtgt ggactatgtt cctgcccatc
     2041 cttccgtcct tcttgggcat cacttcgctt ccatagccgg ggcaggtcct atcgtgggcc
     2101 cgataacggc ggccgccatt ttcgggtggc tacctactta cctatggtgc gtcatcggct
     2161 caatattctt cggaggtgtc cacgacatgg gcgcactggt ctcttccatg cgccacaagg
     2221 gtctctctgt gggagaggtc gttgacaggt ggataggccg ccgaggcaag ctcctcttcc
     2281 tcaccttcac atggctcgct cttgtccttg ttgtggctgt tttccttgaa ctctccgcag
     2341 ggagcttcgc ttcggacccg gccgttgcat tctccgccac tctttacatc ttcatggcac
     2401 ttgttttcgg agtgatggtc tacaagaagg gtatccccct ttccgtatcc accctgatca
     2461 tggtccccat tgtcataggg gcggtctttt tcggcagcta ctctgactgg gtgcaggcca
     2521 acttcaattt cgacatcaac acctggagga tgatccttgc cgtctacatc ttccttgctt
     2581 cggtcctccc ggtatggctg ctgctccagc cccgcgacta ccttgcctcc tacatgctct
     2641 acttctcagt ccttatcggt gcaataggca tgatattcgg ctccaagttc gccacggact
     2701 tccccgcctt caagggattc tacgggggag gtacgaacta catgtggcca atgttattcg
     2761 ttatagtcgc ttgtggagcc atctcgggct tccactccct ggtaggaagc ggtaccacgt
     2821 ctaaacagct caagacggaa aaggacgccc agctggttgg ctacggctcg atgcttcttg
     2881 agggccaggt agccgtaata gccatcggta cggttgtcgt cgccggtggc atcatgcagg
     2941 gaggccccac catcacctac ggccacggca taggccagtt cgccagcctc atcggcatcg
     3001 accccaaggt cggcatgtcg ctaggcctac tcgctctcaa ctccttcctg ttgacctccc
     3061 ttgatacggc caccaggctt gcaaggtacc agctgcagga attcttcgac atgaagctta
     3121 acaagtatgt cgcgacgctg ataggcgtcc ttggagcact gttccttctt tactacaaaa
     3181 ccggcgggaa gcccgcatgg tcccttattt ggcccatctt cggagcatcg aaccagctcg
     3241 tggccgccct tgctcttctg gccgtcgcag tctgggtcaa gaacggtctt aagaaggaga
     3301 acaagttcct catgtacccc ttcttcttca tgatcgcgac caccatcgcg gccctgttcc
     3361 ttctgatcaa gtccaacctc aacaacatgc tcctggcggg gctctcggtg gtcctcatcg
     3421 tgctcgccgt aatgcttctc aaggaagctc gggacgctct tatgggcaag gggccgaaac
     3481 tggaggacac tgcgaaatag tcttcagaaa gttttggctt ggcttcatgg aaaaaagaag
     3541 ggggcggata tccgccccct tttcctgttc tggtcaagag cctggtcctg accgattcaa
     3601 tctcctctct tggggacgct gaaactcttg ctcccccagg acttgccctt gaccggatct
     3661 ccctcgacca gctgaacgaa ccggttgagg cacttcgggc acttgagtcc cggtgaaagg
     3721 tcctccactt cgaactcgtg gcggcagtgg agacatctgt acttcctcac ttgccaacac
     3781 ctccctgcgt catcgtagaa catagtcgta tcctcaggga tattatagcc ggggcccaat
     3841 ggggatacaa gaagagcgga ggttccacag acgaagcagc ctggttgatc tttcagagca
     3901 gcccgaaggt ggaggtcagg gagaagtaag tgcctaaaag gatcaggagc acccccgagg
     3961 ctgcctggat aaccttctgc aagttataga ccgttccgat gctcctggat acggccctgg
     4021 tccccatgga aaagatgacg gagaggattg ttacgggaag tccgctcccg aggccgaaga
     4081 gggccgggaa gaagacccgg gaccctacct ctgtggccag agggatcagt ccgccgaaga
     4141 agagggcggc cgatacgggg cacatggcaa gggcaaagag tactcccatg aagaaagagc
     4201 ccgccatgcc gcggaagggg gcccctgatg agacactgtc aacgatgggt cttctccttc
     4261 tgatctccag gaggtccagg acgaagatgc ccactatcag gagtatgggg ccaatgatcc
     4321 tgttcatgtg acgctgcatg aagatagatg cctctggtat tgccagaagc cccgagatca
     4381 ctatcactcc tgcaatggca aaagccacgc tgcgtcctgc cgagtagagg caccccgaga
     4441 caaggaccct gcggggtgaa agcccttcct tctggatgaa tgaaaaggcg gccatgttcg
     4501 ctgccagcgg gcaggggctt attgaagcca gtaccccgag ccagagggct gacaggaccg
     4561 tcagtacgct caccggttct cacctcccga tcggaaggcc tcgatctccc gaagcatgta
     4621 atccctgaaa gcctcactgt ctccggccag gtcccagaca ccggcaagat ttttccagcg
     4681 gaatatcctg ccccctgatt cctctgcaag aacaacagag gtggagaaga gatcaagctc
     4741 cagaacatac ctctcgttcc ccttctcctc cacgttcaca tcccttatgg aaaatagcga
     4801 aagatcttca gggtccgact cgaaaagttc atatgtcatg ccccttatcc tttcgcatac
     4861 cgaacagggg gcattcccct cgaagaagta gatgaccagt tccaaggggc cctcgggata
     4921 gcctgacccc gatgcggtaa ctgtaccccg gtctcctctc cctccagtag caatatcgcc
     4981 gtaaatatca aggccagcat ttacaagccc ggccagggca agcaaaagga tcaggtaccc
     5041 tgcggtcttt ttatagttca tgacccctcc cgaccttatt ggagtgatga tgggaacttg
     5101 cagtaaatat accataaagg gtatacttgt ctcaagcttt gaggtcaatg ccgtcgacag
     5161 gaggtctctc cagatggtaa agaacggcaa taacgacaag gaaaagacag ccctcaggat
     5221 aacgggaatg acctgcgcgg catgctcagg agcggtggag aggtcccttc ggaaggtcga
     5281 aggggtagat ttcgccgcgg tcaacctcgc tacggagaca gctctggtgg tccatgggag
     5341 agatgtttcc ctcgatgaac tgaaaaaagc cgtcgaaaga gtgggctacg gtgtctccga
     5401 cgaactgccc gaggaggtgg acaggataag gtacctccgt tccaggaggg atctcctgca
     5461 agcccttgtc gttacagtgc ctctcatggt cctgatgctg ctgcatatga cagggtccca
     5521 cctcccctgg ttccccctgg tggagctggc tgcagccggt tttgtggtct tctacgtcgg
     5581 aagggggagc ataaggggag cctggatagc cgtgacccac ttccacacca acatggatca
     5641 ttacgaccca atttgttctg ggcacgtgct
//
LOCUS       DAOPKQ010000078         5573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_15683_length_5573_cov_4.358101, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000078 DAOPKQ010000000
VERSION     DAOPKQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15683_length_5573_cov_4.358101"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK146_03870"
     CDS             complement(<1..388)
                     /locus_tag="PK146_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE91410.1"
                     /translation="MKDPDHGLDPVPTSEGPEEELSGFRPSRLEEFIGQSELKSKLGI
                     YIQAARKRGEPLDHSLFYGPPGLGKTTLAGIIANEMQGQLRVTSGPALERAGDLAAIL
                     SNLQPRDVLFIDEIHRLSPAVEEVLYP"
     gene            complement(398..1000)
                     /locus_tag="PK146_03875"
     CDS             complement(398..1000)
                     /locus_tag="PK146_03875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HPE91411.1"
                     /translation="MILLHSLEGRVSAVETEGIGLDVGGIGFFLLCSKSVLKDAVHGR
                     SMKILTSLQISEAGPSLFGFSDEEERVLFQLLLKGRGIGSRLAATILRTLDFSDIINA
                     IASGNSHLLCQVPGVGKKTSERLCFELRQQIESSGFKSLATMVSPETADTVQFVYEAL
                     GTLGFTRSEARPALARILALKETESPEQLLRDALAELRKS"
     gene            complement(997..1500)
                     /gene="ruvC"
                     /locus_tag="PK146_03880"
     CDS             complement(997..1500)
                     /gene="ruvC"
                     /locus_tag="PK146_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPE91412.1"
                     /translation="MPRVCLGIDPGLGRLGYGIVVQEGSLLRAGEFGCICTEPKISVE
                     KRILQIHERLGSIIGKFNPEIVSVERLFFGKNTTTAENVFQVRGVVLLLAASYGIQVV
                     QPKPSEVKLAICGNGRAEKRQVQMMVFRILNLASFPSQDDAADALAIAATGLAMSDFQ
                     IKTKSGD"
     gene            complement(1506..2258)
                     /locus_tag="PK146_03885"
     CDS             complement(1506..2258)
                     /locus_tag="PK146_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPE91413.1"
                     /translation="MSGHSKWANIKHRKSAQDAKRGKTFQKYIRAVMVASREGGSDAS
                     MNIRLKSAIERARVANVPVDTIEKAVKKGAGDMEGVTYDEITYEGYGSGGVAVLVDVL
                     TDNKNRTASEMRLIFSRSGGSLGESGCVSWIFQRKGVVRLSGKDLDEEQLLMVCLEAG
                     ADDLENNGEGFSVYCDPAVMSEVRETLAEAGYVIEGSEIEKLPKTTVQIKGKQEAEKF
                     LRFLETLEEHDDVQNVYANFDIPDDIMEELAG"
     gene            complement(2266..3027)
                     /gene="nadE"
                     /locus_tag="PK146_03890"
     CDS             complement(2266..3027)
                     /gene="nadE"
                     /locus_tag="PK146_03890"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPE91414.1"
                     /translation="MNYYRDPASLIEKISSWIKEMIGLSGRSGGIVGLSGGVDSAVVA
                     ALLKKVCGDRMLAVMMPCHSMDEDHRDAMKVVEAFDLPWAKVDLDGAYDALLDSLPGD
                     VSYGNGIHLSNVKPRLRMTTLYCLGQKMGLLVCGTGNKAELTMGYFTKHGDSGVDILP
                     LGDLLKGEVNLIARELGVPSGIIKKPPSAGLWVGQTDEDEMGVTYSEIDRYIATGKGS
                     PRSIGIIERALVRSEHKRITPPICLIGEENRKKQG"
     gene            complement(3091..3906)
                     /locus_tag="PK146_03895"
     CDS             complement(3091..3906)
                     /locus_tag="PK146_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPE91415.1"
                     /translation="MKELTVHIIQSDVLWGNPSENIQRMEERLRRIPDGPSLVILPEL
                     WTCSYDNPRMRNHAGQSPAALEMMSSSCRKKGFSMVGGSIPWTAPEGDLLNRSFFLAD
                     SGELVGHYDKAHLFPLLDEPLFFRAGKTPFLFEFMGIPSAIAICFDIRFPEFIRSLAL
                     SGAELVLVPAQWPLARIDHWETLLRARAIENQIFIVGCNRCGEGGGQVYGGRSLAFGP
                     DGTRIVLCPGDREMTAMVPINPSLVRKTRKEHPFTAGRNPLLYHPVTSFDRKA"
     gene            3943..4350
                     /gene="nikR"
                     /locus_tag="PK146_03900"
     CDS             3943..4350
                     /gene="nikR"
                     /locus_tag="PK146_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPE91416.1"
                     /translation="MDKLARFSVSLPAPLLDRFDSWIEARGYRTRSDALRQLIRRFIS
                     ESYWEDGEKIVFGTVTLTYDHHSHDSASEITNLQHEFGDLIICSTHIHADYHHCFESV
                     VFKGKASRVKEFVRQLSGLKDVSSSDPVISSIP"
     gene            complement(4640..>5573)
                     /gene="argF"
                     /locus_tag="PK146_03905"
     CDS             complement(4640..>5573)
                     /gene="argF"
                     /locus_tag="PK146_03905"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPE91417.1"
                     /translation="KGRSFLTLKDFTTEEIQYLLDLSASLKGKKRAGTRGSALAGKNI
                     ALIFEKPSTRTRCAFTVACIDEGGHPEYLGKNDIQLGHKESVKDTARVLGRMFDGIQF
                     RGFKQTVVEELALYSGVPVWNGLTDMYHPTQILADFLTLQENFGKLRGLRLVYVGDGR
                     NNMANSLMIGSAKTGIHFRIAAPADLFPEKALVEECRAVAVGTGSTIEFFEEPQKAVS
                     GADAIYTDVWASMGEEDKLAERRTLLKPYQVNRELMKATGNPDCIFLHCLPAVKGNEV
                     TEEVFESRNSKVFDESENRLHTIKAVMVSTAGNL"
BASE COUNT         1119 a         1664 c         1474 g         1316 t
ORIGIN      
        1 ctgggtagag gacctcctcc actgcagggg acaggcggtg gatctcatcg atgaagagga
       61 cgtccctggg ttggaggttc gaaagaatgg cagcgaggtc accagccctc tcaagagctg
      121 gtcccgaggt gacgcgaagc tgcccctgca tctcgttggc gataatgcct gcaagggtag
      181 tcttccccag cccgggaggt ccgtaaaaca gggagtgatc cagaggttcc ccccgtttcc
      241 gggcggcctg gatgtatatc ccaagcttgc tcttgagctc gctctgtcct atgaactcct
      301 ctagtcttga cggccggaac ccggagagct cctcctccgg tccttcgctg gtgggaacgg
      361 ggtcgagacc atggtcagga tccttcaaga gaacacctca acttttcctc aattcggcca
      421 aagcgtcacg aagcagctgt tcggggcttt ccgtctcttt caaggccagg atcctggcca
      481 gagccggtct ggcttcgctc cgcgtgaagc ccagcgtccc cagggcttcg taaacgaact
      541 gaaccgtgtc tgcagtctcg ggagagacca tggttgcaag gcttttgaaa ccagagcttt
      601 cgatctgctg tctgagctcg aaacataacc tctctgaagt cttctttccc accccgggta
      661 cctggcagag gagatgactg ttcccggagg cgatggcatt gatgatgtca ctgaaatcaa
      721 gggtcctcag gatcgttgca gccaatctcg aaccgatgcc cctccccttg agcagaagct
      781 ggaacaggac tctctcctct tcgtcgctga acccgaaaag ggacggacct gcctcgctta
      841 tctgaagaga ggtcaggatc ttcatggacc tcccatggac ggcgtctttc aggacgctct
      901 tcgagcagag caggaaaaaa cctataccac ctacatcgag cccgatcccc tcggtctcaa
      961 ctgccgaaac cctgccttcc aggctgtgga gcaagatcaa tcaccgcttt tcgtcttgat
     1021 ctggaaatcc gacattgcca ggcctgtcgc agctattgcc agtgcgtcgg ccgcatcatc
     1081 ctggctgggg aaactcgcca ggttaaggat ccggaagacc atcatctgga cctgtctctt
     1141 ctcagccctg ccatttccac agatggccag ctttacctcc gacggcttcg gctggaccac
     1201 ctggatcccg taacttgcag ccaggagaag gaccactccc ctgacctgga agacgttctc
     1261 ggcggttgtg gtgttcttgc cgaagaacaa ccgctcaacg gatactatct cggggttgaa
     1321 cttcccgata atcgatccca gccgttcatg gatctgcagt atcctctttt caaccgagat
     1381 cttcggttct gtgcatatgc aaccgaactc ccccgccctt agcagggacc cttcctgaac
     1441 gactatcccg tatcccagtc tacccagacc aggatctatt ccaaggcaga cgcgcggcat
     1501 cccgttcagc cggctaactc ttccatgatg tcgtcaggga tatcgaagtt ggcgtagacg
     1561 ttctgcacat catcgtgctc ctccagggtt tcaagaaagc ggagaaactt ttcagcctcc
     1621 tgttttccct tgatctggac cgtggtcttc gggagtttct ctatctccga gccctcgatt
     1681 acgtaccccg cctccgccag ggtctccctg acctctgaca tgaccgccgg gtcacagtag
     1741 acagagaacc cttcgccgtt gttctctagg tcgtcggcac ctgcttccag acagaccatc
     1801 agaagctgtt cctcgtcgag gtccttgccg gatagcctga ctacgccctt tcgctggaag
     1861 atccatgata cgcatcctga ttcccccagg gaaccgccgg atctgctgaa gatcagccgc
     1921 atctccgaag ctgtcctgtt cttgttgtcg gtgagcacat cgaccaggac cgccacaccc
     1981 cctgagccgt acccttcgta ggtgatctcg tcgtaggtaa caccctccat gtcccctgcg
     2041 ccctttttga ccgccttttc tatggtatcc acgggcacgt tcgcaaccct ggcgcgttca
     2101 attgcggact tgagcctgat gttcatggag gcgtcggagc ctccctctct ggaagccacc
     2161 atcacggccc tgatgtattt ctggaaggtc ttgcccctct ttgcatcctg tgctgacttc
     2221 ctgtgcttta tattcgccca tttggaatgt cctgacatgt ggtattcacc cctgcttttt
     2281 cctgttttcc tctccgatca ggcagatcgg cggagttatc ctcttgtgtt cggagcgcac
     2341 gagagctctt tctatgatcc cgatgcttct gggcgagccc ttgccggtag ctatgtacct
     2401 gtcgatctcg gaataggtta cccccatctc gtcctcatcg gtctggccga cccagagccc
     2461 cgctgatgga ggtttcttga ttatccccga aggcacccca agctccctgg cgatcaggtt
     2521 gacctctccc ttgagaaggt ccccgagagg gaggatgtcc acgccggaat caccatgctt
     2581 ggtgaaatac cccatggtca actctgcctt gttgcccgtt ccacagacga ggagacccat
     2641 cttctgcccc aggcagtaaa gggtggtcat cctgagtctt ggctttacat tggacagatg
     2701 gatcccgttc ccgtaggaga catcgccggg gagtgaatca aggagggcgt cgtatgctcc
     2761 gtcaagatcc accttggccc agggaagatc aaaggcttcc acgaccttca tggcatccct
     2821 gtgatcctca tccatgctgt ggcaaggcat catgacggct agcattctgt caccgcacac
     2881 ctttttcaga agagcggcca caacggcgga atcgacacct ccgctcagtc cgacgatccc
     2941 tcctgacctg cccgataagc ctatcatttc cttgatccag gaggagatct tttcaatgag
     3001 ggatgctggg tcacggtaat aattcatagg gccactctcc gtaatgtcct gtagtcggat
     3061 gttacaggag ttattctagc acagacacct tcaggccttg cggtcaaagg aagttaccgg
     3121 gtgatagaga agggggttcc ttccagcggt gaaagggtgt tcttttctgg tcttcctcac
     3181 aagtgaggga ttgatcggca ccatggccgt catctccctg tcaccgggac agagtacgat
     3241 acgggtgccg tcggggccga aggccagcga tctcccgccg tagacctgcc ctcctccctc
     3301 tccacaccgg ttacatccca caatgaagat ctggttctcg atcgctcttg cccttagaag
     3361 ggtctcccag tgatcgatcc tcgccaaagg ccattgggcc gggacaagga caagttcggc
     3421 tcccgagagg gccagggaac gtatgaattc ggggaatctg atgtcgaaac agatggcgat
     3481 cgccgagggg atgcccatga actcgaaaag gaagggggtc ttcccggccc ggaagaacag
     3541 cggttcgtcc aaaagaggga acagatgggc cttgtcgtag tggccgacca actcacctga
     3601 atcagccagg aaaaaggagc ggttgagtag atctccctcc ggagccgtcc aggggatgct
     3661 ccctcctacc atggaaaaac cttttttcct gcaggaactg gacatcattt ccagtgcggc
     3721 gggactttgc ccggcgtggt tcctcatcct ggggttgtcg taggagcagg tccaaagctc
     3781 ggggaggatg acaagcgatg gcccatcggg gattctcctc agccgttcct ccatcctttg
     3841 gatattttcg gaagggttgc cccacaggac atcagactgg ataatatgaa cggttagttc
     3901 tttcaagatc attccccctt aaatcagtcg ggaggtaatg ctttggacaa gctggcgagg
     3961 ttcagtgtat ctttaccggc acccctgctg gacaggttcg attcctggat cgaggcccgt
     4021 ggctaccgga ccaggtccga tgcattgaga cagctgatcc ggcgctttat ctccgagtca
     4081 tactgggagg acggtgagaa gatcgttttc gggaccgtta ccctcaccta cgaccaccat
     4141 tcccacgatt ccgccagcga gataaccaac cttcagcacg aattcgggga cctgataatc
     4201 tgttccaccc atattcacgc cgattaccac cactgtttcg agagcgtcgt cttcaagggc
     4261 aaggcttcaa gggtcaagga gttcgtcagg cagttgagcg ggctaaagga tgtatcaagt
     4321 tcagacccgg tcatctcctc cattccctga aagggggagc gatcctcgac tggcggggct
     4381 gatgtcggtg acgaccgtca ccggtcagag gcctcagccc gaaggggcac acttcccctt
     4441 cttcttcctt gccgttcccc ttacaagtat cagcgtccgc ccgcgacact cgggacaccg
     4501 cggttcctgc tcctcttcgg acaggtcgaa caatttaccg catgaggcgc atttgtagct
     4561 cgtctttccc gccatttacc tcacttcctt cagaaaattc ggaccctgcc cggggggcgg
     4621 ggcctgaatt catggtcaat taaaggttgc cggccgttga taccatgaca gccttgatgg
     4681 tgtggagcct gttctccgat tcatcgaaca ccttggagtt cctggattca aagacctcct
     4741 cggttacttc gttgcccttc actgccggga gacagtgcag gaatatgcaa tcgggatttc
     4801 ccgtggcctt catcagctcc ctgttgacct ggtagggttt gaggagggtt ctcctctcag
     4861 cgagtttgtc ctcttctccc atcgatgccc agacatcggt atagatggca tccgcgccgc
     4921 tgaccgcttt ttgaggttcc tcgaagaact cgatggtgga tcctgttccg accgcaacgg
     4981 cccgacattc ctccaccaga gccttttcag ggaagagatc ggcaggggcg gctatcctga
     5041 agtgaatccc cgtcttcgcg gaaccgatca taagcgagtt ggccatgttg ttccttccat
     5101 caccgacata gaccagcctt agccccctga gcttgccgaa gttctcctga agcgtcagga
     5161 aatcggccag gatctgggtc gggtgataca tatcagtgag gccgttccat accggcacac
     5221 cggagtaaag cgcaagctcc tccactacgg tctgtttgaa accgcggaac tggatcccgt
     5281 cgaacatcct tcccaggacc cttgcggtgt ccttgacgga ttccttgtgt cccagctgga
     5341 tgtcgttctt gccgagatac tcgggatggc ctccctcgtc tatgcaggcc acagtgaatg
     5401 cacagcgtgt cctggtggag ggtttttcga agataagggc tatgtttttg ccggccaggg
     5461 cgctccccct ggtcccggcc cgcttcttac ccttgagaga ggcagaaagg tcgagcaggt
     5521 actggatctc ttcggtggtg aaatccttca gggtcagaaa acttcttcct ttg
//
LOCUS       DAOPKQ010000079         5526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_15890_length_5526_cov_4.511241, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000079 DAOPKQ010000000
VERSION     DAOPKQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15890_length_5526_cov_4.511241"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..500)
                     /gene="rplI"
                     /locus_tag="PK146_03910"
     CDS             complement(45..500)
                     /gene="rplI"
                     /locus_tag="PK146_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPE91418.1"
                     /translation="MKVILLTDIPKLGKKGDLIDVAEGYGRNYLVPRKMAEEATREKL
                     EEWNQRQRTKEVRARREEEEARNHRALLQGKQVVLRATAGEKGKLFGSVTAAQVAESI
                     QERYKMPVDKKDIKLPSPVKELGSYNFSVRLYQGVEAEMILQVEDERVE"
     gene            complement(497..1456)
                     /locus_tag="PK146_03915"
     CDS             complement(497..1456)
                     /locus_tag="PK146_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HPE91419.1"
                     /translation="MDRTRALVESSLLVALSAMLFLAGHFLPIVGMAFSLVCPAPLVV
                     LGLRHSLGRAVLGVAVATVITAAFTGAVGALFFCFGFGFLGIALGALGRRFGRAVDIV
                     LYGILVSIGSKLLLMVIAIKLTGINPFGLEEAEIMPVVERIASLYSGLGMSEEALSLA
                     KEQMRATLSLIPLIFPALLVMAATVDCFLSYIVSAAVLKRLGRETLPGLPEFSRWRFP
                     KSIFWALIASLALHLTGAYYPSMAFLQRTALNLRLLVTALFFFQGLAVGWSFMQARGL
                     GRFLRVALTVLVFVVSFLAQIVLVLGIIDMWFDLRSRFRGGRA"
     gene            complement(1532..1783)
                     /gene="rpsR"
                     /locus_tag="PK146_03920"
     CDS             complement(1532..1783)
                     /gene="rpsR"
                     /locus_tag="PK146_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPE91420.1"
                     /translation="MAFSPRNSGNKRNKRRKPKVCMFCVDKIEHIDYKDVEKLRKYIS
                     ERGKIVPRRVTGNCAKHQRQLTEAIKRVRYLALLPYSAE"
     gene            complement(1819..2304)
                     /gene="ssb"
                     /locus_tag="PK146_03925"
     CDS             complement(1819..2304)
                     /gene="ssb"
                     /locus_tag="PK146_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPE91421.1"
                     /translation="MARGFNKAILMGNLARDPEVRYTTSKQAVARLTVAVNRQWKNRD
                     GEVQDQADFIPVVVWGSQAENCERYLRKGSPVLVEGRIQVRNYEAGTGERRWVTEVIA
                     QGVTFLGTRRESSEGFSPDPDQGSRGGSLREKGFGDDFVMDISEMDDTKDDGEDADIP
                     F"
     gene            complement(2317..2601)
                     /gene="rpsF"
                     /locus_tag="PK146_03930"
     CDS             complement(2317..2601)
                     /gene="rpsF"
                     /locus_tag="PK146_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPE91422.1"
                     /translation="MRPYEMVTIFQADLEDHKSAVEEIEGVVRNLGGEIEKSSSWGKR
                     RFAYPIQKQTDGFYAVLSFSLEPSQVSELERLLKLKTQVLRHLVIALDEA"
     gene            complement(2678..2983)
                     /locus_tag="PK146_03935"
     CDS             complement(2678..2983)
                     /locus_tag="PK146_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HPE91423.1"
                     /translation="MIRLRWVVLITAGLCFLAIVGTAYILELKKISRLGSIVDERMDR
                     LVAVTRDVQVLREKIIFYRTPEGVARLAREQFNLTYPGEQIFRIEVVSEDSLPGDSP"
     gene            complement(2986..4665)
                     /gene="argS"
                     /locus_tag="PK146_03940"
     CDS             complement(2986..4665)
                     /gene="argS"
                     /locus_tag="PK146_03940"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPE91424.1"
                     /translation="MKKDIVAVITEMLEISIGKIACEKGIPLEARPLVELERGRREGQ
                     GDWATNAAMQWARTFGEKPLDVARRIVSLLPGSPLVERVEVAGPGFINFFLSRSWMTG
                     TVEHILGCGPDFGRVNSGGGRRVQVEFVSANPTGPLHVGHGRGAAVGDICARILSFAG
                     WKVEREYYINDAGLQMELLGRSTQSRYFEICDRSEEAPFPEDGYKGGYIFDLARNVLD
                     SEGERFLGMPLKESLPFFTEYAGRVILEGIKKDLEDFGVKFNVWFSEKSLYDGVRVPS
                     MLETLKKNGFAFDADGATWFKATAFSDDKDRVLIRSNGAPTYFASDIAYHKDKFDRGF
                     DMVIDVWGADHHGYIPRMKAAVQALGRKEEDLQVLLIQFVNLLRGGEQVSMSTRSGEF
                     VTLREVIDEVGVNATRYFFAMRRSDSHLDFDLDLAKSASTDNPVYYVQYAHARIHSIF
                     REATARGITVPDPSGADLSVLVEPEEIALVKRLSAFPEEVEKAANDLAPHRIVFYAYD
                     LAGDFHSFYNAHRVLGVGAELEGARLLLAEASRTVLANALSLLGISAPEKM"
     gene            complement(4685..>5526)
                     /locus_tag="PK146_03945"
     CDS             complement(4685..>5526)
                     /locus_tag="PK146_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91425.1"
                     /translation="WDPRVPLGDQSPATVRAWERPVLSLTPPTGKSFMFEIGQAARPG
                     STPPRLWGRTVYSLEGSSPSRRVLPAGGPGDHRLSFDWTVDVSSDGMATGELVITARG
                     AWVDTLQLKGSIEKDQVTEILGSFSWPSIPGFSFEGALMEPAGTGFRIKVPLNARIAI
                     PGGEGLLLRMPSVVMPWQSSIAEKGAGGGIRFPFVYEQSVSIGLPEGFDVMVLPATRP
                     YESGTVKLEESMRVRRGRTLLAEQKTVVSTARLDEQARQALTNAVRQGLGWTGTTVPM
                     KKR"
BASE COUNT         1140 a         1733 c         1402 g         1251 t
ORIGIN      
        1 tctcggcttc gatgctatgg ggcggtatcc tctcgaagag ctggtcactc aaccctttcg
       61 tcctccacct gaaggatcat ctctgcttca actccctgat aaagtcttac ggaaaagttg
      121 taggagccca actccttcac aggcgagggg agtttgatgt ccttcttgtc gaccggcatc
      181 ttgtaccttt cctggatgct ttctgccacc tgggctgctg ttacacttcc gaatagcttc
      241 cccttctctc ctgctgtcgc tctgagaact acctgcttcc cctggagaag cgccctgtgg
      301 ttcctggcct cctcctcttc gcgtctggcc ctgacctctt tcgttctctg gcgctggttc
      361 cactcttcga gcttctccct ggtcgcctct tcggccatct tcctgggaac taggtagttc
      421 cttccgtagc cttcagccac atcgatgagg tcgccctttt tccccagctt gggtatatct
      481 gtcaacagga tcaccttcat gctcttcccc cccgaaacct ggaacgcaga tcgaaccaca
      541 tgtcaataat acctaaaaca agaactatct gtgcaaggaa tgatacgaca aatacaagca
      601 ctgtcagggc aacccttaaa aaacggccca ggccccttgc ctgcatgaag gaccatccta
      661 cggccagccc ctggaagaag aaaagagctg taaccagaag gcgcaggttc agagccgtcc
      721 tctgcaggaa tgccattgaa gggtaatagg cccccgtcag gtggagagcc aaggaggcga
      781 taagcgccca gaagatgctc ttagggaaac gccatcggct gaactcgggg agtccaggga
      841 gggtctccct gccgagcctt ttcaggaccg cggcgcttac aatatagctc aggaaacagt
      901 caaccgtcgc agccattaca aggagtgcag gaaagatcaa ggggatcagc gacagagtgg
      961 cgcgcatctg ctctttcgcc agggaaagcg cctcctctga catgcccaga cctgagtaga
     1021 gcgaggcgat cctctcgacc acgggcatta tctccgcttc ctcaaggccg aagggattaa
     1081 tgccggtaag tttgatggct attaccatga ggaggagctt gctcccaatg gagacaagga
     1141 taccgtaaag gactatgtca acggctctcc caaaacgtct ccccagggcc ccaagggcga
     1201 tcccgaggaa gccgaacccg aaacagaaga agagagcccc caccgcccct gtaaaggcag
     1261 ccgtaatgac cgtagcgacc gcaacaccaa ggaccgccct cccaaggctg tgcctgagac
     1321 cgagaacaac aaggggggca ggacatacca gcgagaaggc catccctact atgggcagga
     1381 agtggcccgc cagaaaaagc atggctgaga gggctacgag aagcgaagat tcgaccagag
     1441 ccctggtcct gtccattact tacaacctcc ctaacagaac ggggggagaa gcctcggctc
     1501 ctccccccgc gaggaacacg ggttatcgtc tctactcggc gctgtacgga aggagcgcaa
     1561 ggtaacgcac ccttttgatc gcttccgtga gctgcctctg gtgtttggca cagttccccg
     1621 tcacccttct ggggactatc tttccacgct cgcttatgta cttcctgagt ttctcaacat
     1681 ccttgtaatc tatgtgctcg atcttgtcca cacagaacat gcagaccttg ggtttccttc
     1741 tcttgtttct tttgtttccg ctgttccttg gtgaaaaggc catcacgatc ctccttccct
     1801 taagctgttc tctagacgtc agaaggggat atcagcatcc tccccatcgt ccttggtgtc
     1861 gtccatctcg gagatatcca ttacgaaatc gtcaccgaaa cccttctcac ggaggcttcc
     1921 gccccttgat ccctgatcgg ggtcgggtga gaacccctca ctgctctccc tacgggttcc
     1981 caggaatgtg acaccctggg ctatgacctc tgtcacccag cgcctctctc ctgttccagc
     2041 ctcgtagttg cgaacctgga tccttccctc cacgagaaca gggctcccct tccggagata
     2101 tctctcgcag ttctctgcct gggatcccca aaccactaca gggatgaagt ccgcctggtc
     2161 ctggacttcc ccgtccctgt tcttccactg gcgattgacg gccaccgtga gcctggcaac
     2221 agcctgcttt gaagtggtat agcgcacttc cgggtcccta gccagattcc ccattaagat
     2281 cgccttgttg aaacctctgg ccatcggtat cactccctag gcttcatcca gtgctatcac
     2341 caggtgcctc aggacctggg tcttgagctt cagcaatcgt tccagttcgc tgacctgcga
     2401 tggttcgaga ctgaaagaga gaaccgcgta gaacccgtcg gtctgtttct ggatgggata
     2461 ggcaaaacgc ctcttccccc aggaactgct cttttcgatc tcgcctccca ggttgcgcac
     2521 gaccccctct atctcctcca cagccgactt atgatcctca agatcggcct ggaagatcgt
     2581 aaccatttca tacggtcgca cgtcattcac ctcctcccct tggcctatgg tctccccggt
     2641 tccggagaga cagggacata cggcagaaca ttatacgcta cggagagtct cccggcaagg
     2701 agtcctccga caccacctcg atccggaaga tctgctcgcc agggtaggtc aggttgaact
     2761 gctcccttgc gaggcgagcc acaccctccg gggtcctgta gaagatgatc ttctccctga
     2821 ggacctgtac atcccttgta acggcgacga ggcggtccat cctttcatcg acgatggacc
     2881 caagcctgct tatctttttt agctccagga tataagccgt tccgacgatc gccaggaaac
     2941 agagacctgc tgttatcagg accacccagc gcagtcggat catgcctaca tcttctccgg
     3001 tgcgcttatc ccaagcagtg aaagagcgtt ggccagaact gtccgggagg cttcagccag
     3061 aagcagcctc gctccctcca gctccgcccc cacccccagt acccggtggg cattgtagaa
     3121 ggagtggaag tcacccgcca ggtcgtaggc atagaagact atcctgtggg gggcaaggtc
     3181 gttcgcggcc ttctccactt cctcgggaaa ggccgagagt ctcttgacaa gggcgatctc
     3241 ctccggttct accaggacgg aaaggtctgc cccggaggga tcggggactg tgatacccct
     3301 ggccgtcgcc tccctgaaga tgctgtgaat ccttgcatgg gcgtactgga cgtagtaaac
     3361 agggttgtcc gtggaagcgc tcttggccag gtccaggtcg aagtcaaggt ggctgtctga
     3421 tcgtctcatg gcaaagaagt atctggtggc gttcactccc acttcgtcta tgacctccct
     3481 gagggtgaca aactcacccg acctggttga catggaaacc tgctctccac ccctgaggag
     3541 attcacgaac tggataagga ggacctggag atcctcctcc tttctcccca aagcctgcac
     3601 tgcagccttc atcctcggga tgtagccgtg gtggtcggca ccccacacat caattaccat
     3661 gtcaaacccc cggtcgaact tgtccttgtg ataggctata tcggaggcaa agtaggtggg
     3721 ggcaccgttg ctcctgatga gtaccctgtc cttgtcgtcg gagaaggccg tggccttgaa
     3781 ccaggtagcc ccatctgcat cgaaggcgaa accgttcttc ttgagggtct ccagcatgct
     3841 cgggaccctt actccatcgt agagagattt ctccgagaac cagacattga attttacccc
     3901 gaaatcttcg aggtccttct tgataccttc caggatgacc cttcctgcgt actcagtaaa
     3961 aaagggcagg gactccttga ggggcattcc aaggaaacgc tccccttcag aatccagaac
     4021 attccgtgca agatcgaaga tgtatcctcc cttgtagcca tcctcgggga agggagcctc
     4081 ctcggaccgg tcgcatatct caaagtacct tgactgggtt gatctcccca ggagctccat
     4141 ctggagtcct gcgtcattga tatagtactc cctctcaact ttccacccgg caaaggagag
     4201 gatcctcgcg catatgtcgc ctacggccgc ccctcttccg tgaccgacat gcaggggccc
     4261 cgtcgggttg gcgctgacga attcgacctg gaccctgcgt cctcccccgc tgttgaccct
     4321 gccaaagtca ggaccgcatc ccagaatgtg ctctactgtc ccggtcatcc aggaccttga
     4381 gaggaaaaag ttgatgaaac ccggtcccgc tacttcgacc ctttccacga gggggctccc
     4441 cggaagaagg gatactatcc tgcgggccac atccaggggt ttctccccga aggtcctcgc
     4501 ccactgcatc gcggcgttgg ttgcccagtc tccctgcccc tcccttcttc ccctctctag
     4561 ctcaacgagg ggtcttgcct caagggggat ccccttttcg caggcgatct tccctatgga
     4621 tatctccagc atttctgtaa taacagcgac aatatccttt ttcatggctc ctccccgctt
     4681 tcggtcacct tttcttcatg ggtactgtgg tcccggtcca acccaggccc tgtctaacgg
     4741 cattcgtcag ggcctgcctg gcctgttcat ccaatctcgc cgttgagacg acggtcttct
     4801 gctccgccag aagggtccgt cctctcctga ccctcatgga ctcctccagt ttgaccgttc
     4861 ctgattcata aggtcgggtc gccggcagga ccatcacatc gaatccttca gggagcccta
     4921 tactgactga ctgctcgtaa acgaagggga accggatacc ccctcccgct cctttctctg
     4981 ctatggaact ttgccagggc atcaccactg agggcatcct gagcagcagg ccctcacctc
     5041 cgggtatggc gatccgggca ttcaagggaa ccttgatcct gaaacctgtt cctgccggtt
     5101 ccataagggc cccttcgaaa gaaaatccgg ggatggaggg ccatgaaaag gagcccagga
     5161 tctctgtgac ctggtccttt tcgatggacc cttttagctg taatgtatcg acccatgcac
     5221 ccctggcggt gatcaccaat tctccggtgg ccattccgtc tgaagataca tcaacggtcc
     5281 agtcaaacga aagcctgtga tctcccgggc cgcccgcggg cagtaccctt cgtgatgggg
     5341 agcttccctc cagggagtag actgtcctcc cccataatct gggaggcgtc gagcccggtc
     5401 tggcggcttg tcctatctcg aacatgaagg attttcccgt aggcggggtt agcgaaagga
     5461 ccggtctttc ccatgccctt actgtggcgg gcgactggtc tccgagggga acacggggat
     5521 cccaga
//
LOCUS       DAOPKQ010000080         5501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_15981_length_5501_cov_3.960521, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000080 DAOPKQ010000000
VERSION     DAOPKQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15981_length_5501_cov_3.960521"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK146_03950"
     CDS             <1..240
                     /locus_tag="PK146_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HPE91426.1"
                     /translation="MEAEERDVYIDRLEELTEAGACGTAAVITPIGGIEYKGKLHVFY
                     SETEVGPVTRKLYETLTGIQFGDIEAPEGWIFKVK"
     gene            complement(273..947)
                     /locus_tag="PK146_03955"
     CDS             complement(273..947)
                     /locus_tag="PK146_03955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPE91427.1"
                     /translation="MRNYYSGKLNAVRLLEVYQTGIERVRQYLQAEIDFVTAKLSGRE
                     RVLELGAGYGRIMREVSPFAARVLGLDISEDSVLFGKEYLRDNPNCKLEIADAHLIEY
                     RSEFDVVLCLQNGLSAIKGDPINLVTRGIRALVAGGSAFFSTYSANFWGPRLEWFMEQ
                     ADKGLLGEIDWERTGKGRIVCRDGFSATTFSVEDLEKMGQTTGLPYLVEEVDGSSLFL
                     VIKRTG"
     gene            1188..2057
                     /locus_tag="PK146_03960"
     CDS             1188..2057
                     /locus_tag="PK146_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE91428.1"
                     /translation="MQLFIEHLINGLAVGSIYALMALGLAMVYGILRILHVAHAGVYA
                     IGGYVGLFAFQATDSFFVAIAAAMVVCAIVGVAIEKVIYYPLLKYPPYVPLIASIAIF
                     LSIEEICRILAGPYILGFPRRLALSPLTLGSVSIGGMQILVLLTSFAILLVLWFISER
                     TELGLAMQATSQDMAMASAMGVNTKRIISITFAMGSAVAAVAGVLVGIYYNQVYPTMG
                     AVPAYKTLAIIVVGGLGSIPGAVLAAILLGLAEALLIGYAAVPLPRDALAFIAMIIVF
                     MIRPQGLLGRKTR"
     gene            2071..2943
                     /locus_tag="PK146_03965"
     CDS             2071..2943
                     /locus_tag="PK146_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE91429.1"
                     /translation="MGGYFTTVATMIGIYALVASGLNIIVGFAGQISLGHAAFFGIGA
                     YSTALLCTKLGLSFWWSLPLATLITGLIGALLGIPSLRVKDDFLAITTIGINFIVRSI
                     FLYVPFFGGALGIGGIPTPTLLDTRLRGEYYLTLVLVLLALTTWFVHRFARSWTGLAT
                     EAMREEETAAGAMGISHVRFKILAFTIGSAMAGLGGVLYAHFVNFISPDDFGFPVSIM
                     LLSMVVLGGMGKIWGPILGAIVLGSLPEVFRPLVQYRMLLYGAALLLVIRYQPQGILG
                     EGSLLRRLFPRGRR"
     gene            2946..3716
                     /locus_tag="PK146_03970"
     CDS             2946..3716
                     /locus_tag="PK146_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91430.1"
                     /translation="MAEKGMILRVEGMSRYFGGLKAVDDVSFHVSRGEILGLIGPNGA
                     GKTTCFNVISGVYRPTAGKIFLEEERIDGLPAHAIASRGVGRTFQIVRPFAGMTVLNN
                     VMVGLGMQRYSSFLKSCPVFGDSQDREEAMAFLREVGIEGLADGSSSVLSLGNLRRLE
                     IARALALRPRLLLLDESFSGLSEEEIQQMMELVRNIRERGFSVLLIEHNMRVAMSLCH
                     RLVVLDHGKKLAEGSPGEIRTDERVIEAYLGRGASAHA"
     gene            3709..4443
                     /locus_tag="PK146_03975"
     CDS             3709..4443
                     /locus_tag="PK146_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91431.1"
                     /translation="MLKVENLTVAYGDIVALEDVSFSVDDGEVVSIIGANGAGKTSLL
                     NTLMGLVPSRSGRVVYIDPSETGNGRGHDILPLSAHERAGMGIRIVPERARVFPNLTV
                     EQNLRMGVYGLSGRINVEEAFGEVFDLFPVLKERRWQAGQTLSGGEQQQLAIARALVS
                     GPKMLLVDEISMGLMPKLVDLVFSVLKRLNEDKGLTILLVEQNAVQALAIAHRGYILE
                     TGRIALEGKASDLMEDTKVREAYLGG"
     gene            4525..>5501
                     /locus_tag="PK146_03980"
     CDS             4525..>5501
                     /locus_tag="PK146_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE91432.1"
                     /translation="MKRLVSLVLALALIIGSVGVVSAADTIRIGLMAPLTGFAAADGL
                     SVLQSVQLAVDQVNAKGGVLGKQVELFYVDDAANAKEAVGLARKLVQMDKVVAVVGGS
                     YSMPTRAVAPLFQEFEIPLVAGYAVHPDITVAGDFCFRNGFLGTVEGRGAGVAAVDML
                     KAKTVGMLTMDNDFGRTMAEGFEQYVVSKGAKVVYSQVYPLGEKDFMPYLTAIKEADP
                     DVVLASGYYAEAAGIVKQAYELKLRTQILGEEGYDSPKFVELAGEEAANGTIIVTNLD
                     RDDERPVVQAFIKEYRERYGMEPDMVGASNYDAFMIITDAIERAGTTDA"
BASE COUNT         1155 a         1450 c         1636 g         1260 t
ORIGIN      
        1 atggaggccg aggagaggga tgtctatatc gacaggctcg aggaactgac agaggccgga
       61 gcctgcggga ccgccgcggt cataaccccc atcggtggaa tagagtacaa gggcaagctt
      121 cacgtctttt acagcgagac cgaggtcggt cctgtgacca ggaagctcta cgaaaccctg
      181 acaggaatcc agttcggtga catcgaggct cccgaaggat ggatcttcaa ggtcaagtag
      241 tgaagacctg ttccaggagc tgatcgttca ggctatccag tccttttgat gaccaggaag
      301 aggcttgagc catcgacttc ctcaacgagg taggggaggc cggtggtctg gcccatcttt
      361 tcaaggtcct caacggaaaa ggtggtggct gaaaagccgt cccggcatac tatcctcccc
      421 ttcccggtcc tctcccagtc gatctcgccc aggagccctt tgtcggcctg ctccatgaac
      481 cactcaagcc tcgggcccca gaaattggcg ctgtaggtgc tgaaaaatgc gctgcccccc
      541 gcaaccaggg ctctgatccc tcttgtgacc agatttatgg gatcaccctt gatcgcagaa
      601 agaccgttct ggagacagag aaccacgtcg aactccgaac ggtactcaat gaggtgggcg
      661 tcggcgatct ccagtttgca gttggggttg tccctgaggt actcctttcc gaaaaggacc
      721 gaatcctccg agatgtccag accgagaacg cgggctgcga agggcgagac ctccctcatt
      781 atcctcccgt atcctgcgcc cagttcgaga accctttccc gacccgatag ctttgctgtc
      841 acgaaatcta tttcagcctg aaggtactgt ctgaccctct cgatccccgt ctggtagact
      901 tccagaagcc tgacggcatt cagcttgccg gagtaataat ttctcaccgc actccctccc
      961 tggacagcca tgttcccgga agctagatac ttgactgttc gcagggcaat gatatcttaa
     1021 ataggctcaa tatttaatct cggagggtcc tcgaatgggt gtaacccagc ctatcgcagt
     1081 ccttcaggag gtcccttcca ttcaggattt atcccgatct ccagggttcc ttgcccgaga
     1141 cttcgaagag cttcaaaagg caatgatcac gacaagaggg gttggacatg cagttattca
     1201 ttgagcatct catcaacggt cttgcagtcg gttccattta cgccctcatg gccctggggc
     1261 tggcgatggt ctacgggatc ctcaggatcc ttcatgtcgc tcacgcaggg gtatatgcga
     1321 taggaggata cgtggggctc ttcgctttcc aggccaccga cagttttttc gtagccattg
     1381 cggccgccat ggtggtatgc gccatagtgg gggtggccat agaaaaggtg atctactacc
     1441 cacttctgaa atatcctccc tacgtgcccc tgatagcaag catagccata tttctttcca
     1501 tcgaggagat ctgcaggata ctggcagggc cttacatact cgggttcccc aggaggctgg
     1561 ccctcagccc actcaccctg ggaagcgtca gtataggggg gatgcagatc ctggtactgc
     1621 tcacctcttt tgccatacta ctggtccttt ggttcatatc ggaaagaacg gaacttggac
     1681 ttgccatgca ggctacctcc caggatatgg ctatggccag tgccatgggc gtcaacacaa
     1741 agaggatcat ctcaattacc tttgccatgg gttcggccgt agctgcggtg gcgggggtac
     1801 tggtgggcat ttattacaac caggtctatc cgaccatggg agcggttccg gcctacaaga
     1861 ccctggcgat catagtggta ggaggtctgg ggagcatccc gggggcggtc cttgcagcaa
     1921 ttcttctcgg actggccgaa gccctgctta taggttacgc tgcggtccct cttcccaggg
     1981 atgccctggc attcattgcc atgataatcg tcttcatgat ccggccccag ggtctcctgg
     2041 gcaggaagac cagataagga gactggagct atgggtggct atttcactac cgttgccacg
     2101 atgataggga tctacgcact ggttgcatcg ggccttaaca tcattgtcgg gttcgccggc
     2161 cagatctcgc tgggccacgc cgcctttttt ggtattggtg cttactccac ggcactgctg
     2221 tgtacgaaac tcggactctc cttctggtgg agtcttcccc tggccacgct gatcaccgga
     2281 ctaatcgggg cccttctggg tatacccagc cttcgcgtaa aggatgattt cctggccata
     2341 accaccattg ggatcaactt catcgtaagg agcatcttcc tttatgttcc tttctttgga
     2401 ggggctctgg gcataggggg gataccgacg cccaccctgt tggacaccag gctcagaggg
     2461 gagtactacc tgaccctggt gctggtcctt ctggccctga cgacctggtt cgtacaccgt
     2521 tttgcccggt cctggacagg tcttgcgacg gaggccatga gggaggagga gaccgccgcc
     2581 ggggccatgg gtatctccca cgtacgcttc aagatactgg ccttcaccat cgggagcgcc
     2641 atggcaggtc ttggcggggt cctctacgcc catttcgtaa atttcataag ccccgacgac
     2701 ttcggttttc ccgtttcgat catgctcctc tccatggtcg ttctcggggg catggggaag
     2761 atctggggtc ccatcctggg agccattgtt ctgggatcgc tgcctgaagt cttcagaccc
     2821 ctggtccagt accgaatgct cctctacggc gctgcgctcc tgctggttat ccgttatcag
     2881 ccacagggga tcctgggaga gggcagcctt ttacgacggc ttttcccgcg ggggaggcga
     2941 tgacaatggc ggaaaagggc atgatcctcc gtgttgaggg gatgagccgg tattttgggg
     3001 ggcttaaagc cgttgacgat gtttcctttc acgtaagcag gggggagatc cttggcctga
     3061 taggacccaa cggggcaggc aagacaacgt gcttcaatgt catctccgga gtatacagac
     3121 ctacggcggg gaagatattt cttgaggaag aacggatcga cgggctgcca gcccacgcca
     3181 tcgcatccag gggcgtgggg cgtaccttcc agatcgtgag accctttgcg gggatgacgg
     3241 tgttgaataa cgtcatggtc ggcctgggga tgcaaagata cagttccttc ctgaagagct
     3301 gtcctgtttt cggggactcc caagaccgtg aagaggctat ggcattcctg agggaagtgg
     3361 ggatcgaagg gcttgccgac ggatcttcat ccgtcctctc cctggggaac ctcaggcgcc
     3421 tggagatcgc gagggccctg gcgttgaggc ccagactcct tcttcttgac gaatctttct
     3481 ctggtctcag cgaagaggag atccagcaga tgatggaact ggtaaggaac atccgtgagc
     3541 ggggattctc ggtccttctg atagagcaca acatgagggt ggcgatgtct ttgtgccaca
     3601 ggctcgttgt gctggaccac ggcaaaaagc tggccgaagg gtctcccgga gagatcagga
     3661 ccgatgaaag agtgatcgag gcctatctgg ggaggggggc atcggcccat gcttaaagtc
     3721 gagaacctta ccgttgccta cggggacata gttgcactgg aagacgtctc cttctcggtg
     3781 gatgacgggg aagtggtctc cattattgga gccaacggcg caggaaagac gtcactcctg
     3841 aacaccctga tggggcttgt cccttcaaga tccggaaggg ttgtctacat agatccttcc
     3901 gaaaccggta acggcagagg acatgacatc ctcccccttt ctgctcatga aagggcaggt
     3961 atggggatcc gtattgtgcc agaaagggca agggtttttc ctaaccttac ggtggagcag
     4021 aatttgagaa tgggtgtcta cggtctttca ggcaggatca atgtagaaga ggcgttcgga
     4081 gaggtctttg atcttttccc ggtcctgaag gagaggagat ggcaggccgg acagacccta
     4141 tccggaggtg aacagcagca gctggccatt gccagagccc tggtgtcggg accgaagatg
     4201 ctcctggtgg atgaaatttc catgggtctc atgccgaaac tggttgacct cgtcttctct
     4261 gtccttaaga ggctgaacga ggacaagggg ctgaccatac tgctggttga gcagaacgcg
     4321 gtccaggctt tggccatagc ccacaggggt tatatcctgg agacaggcag gatagccctt
     4381 gagggaaagg cctcagatct aatggaggat acaaaggtca gggaagcata tcttgggggg
     4441 tgatgatacc ggagggggga gcatcgggat atcccggttc ttgagagagt ctgtacatag
     4501 agaatcgaaa gggagggttt tttcatgaaa agacttgttt cgctggttct tgcgctggcc
     4561 ctgatcatcg gttcggtcgg ggtcgtatcg gccgcggaca cgatccggat cggtctgatg
     4621 gctcctctaa cggggtttgc tgccgctgac ggattgagtg ttctgcagtc ggtccagctc
     4681 gctgttgacc aggtcaacgc caagggtggg gtcctcggaa agcaggtcga acttttctac
     4741 gtggatgatg ctgctaacgc caaggaagct gtggggcttg ccaggaaact tgtccagatg
     4801 gacaaggtcg ttgcggtagt cgggggatcc tacagcatgc cgaccagggc tgttgcacct
     4861 ctcttccagg agttcgagat cccgctggtc gcaggatatg ctgtccaccc ggatatcacc
     4921 gttgccggtg atttctgctt caggaacggc ttcctcggga ccgttgaagg tcggggtgcc
     4981 ggagttgctg ccgtcgacat gctgaaggca aagaccgttg gcatgctcac catggacaac
     5041 gacttcggaa ggaccatggc tgagggtttt gagcagtacg ttgtctccaa gggggccaag
     5101 gttgtttaca gccaggtcta tcctcttgga gagaaggatt tcatgcctta cctgacagcc
     5161 attaaggaag ccgaccccga tgtggttctt gcaagcggtt actatgcgga ggctgcaggt
     5221 atagtgaagc aggcctacga actcaagctc aggacgcaga tactagggga ggagggttac
     5281 gattctccca agtttgtaga actagctggt gaagaggctg ccaacggaac gataatcgtg
     5341 accaacctcg accgtgacga cgagagaccc gttgtccagg ccttcatcaa ggagtaccgg
     5401 gagcgttatg gcatggaacc cgacatggtc ggagcctcca actatgacgc tttcatgatc
     5461 atcaccgatg cgatcgaaag ggctgggacc accgacgctc a
//
LOCUS       DAOPKQ010000081         5443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_16213_length_5443_cov_4.422049, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000081 DAOPKQ010000000
VERSION     DAOPKQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16213_length_5443_cov_4.422049"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK146_03985"
     CDS             <1..320
                     /locus_tag="PK146_03985"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="catalyzes production of carbamoyl phosphate from
                     bicarbonate and glutamine in pyrimidine and arginine
                     biosynthesis pathways; forms an octamer composed of four
                     CarAB dimers; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HPE91433.1"
                     /translation="LASGASTYKLPFGHRGTNHAGKECAGGRAFVTSQNHDYAVLDDS
                     LEGTGLEITHRNLGDGSVEGLKSRDMRVVAVQYHPEGAPGPRDDDRFFEEFLFMCDGG
                     ATL"
     gene            317..3472
                     /gene="carB"
                     /locus_tag="PK146_03990"
     CDS             317..3472
                     /gene="carB"
                     /locus_tag="PK146_03990"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPE91434.1"
                     /translation="MKTPGGPILVLGSGPIRIGQAAEFDYSGSQACRALREMGYHVIL
                     LNSNPATIQTDTAMADVVYIRPLEAEVVERILATHRPSGVIATLGGQVALNLCVELQR
                     RGLWERFGVEVLGTSPRSVQRSEGREDFRQAMLGISEPVIESIYVDNVESALQFASVS
                     SFPFIIRPDYTLGGTGGGVAFDPDSLAERVAEGLRLSPVSRVLVERYLQGWREIELEV
                     IRDGAGNRLCVCGMENVDPMGIHTGDSIVVAPVLTLSDGQWQRLRSAAFRIVDELEIM
                     GACNVQFALSPDAGEYAVIEVNPRASRSSALASKATGYPIARVAARIAAGSLLTEMTN
                     PVTGVGSALSEPALDYVVVKFPRWPFEKFPGAENRVGTRMKSTGEVMALGRTFIEALS
                     KAVRSLDLPGGIVDPRMGLATIEDLEEDLSLPTHRRLWSIFELLGRGLPPRDISARSG
                     IHPFFIDQLARIVSVLGELKEREPDETLLRKVKSMGLSDRDIASASGSDWRRIRDARL
                     SLGIERGYREVDGSAGETPAGSGYYYGTCGASDDGIRHDSRETYAVLGSGPIRIGQGV
                     EFDYCCVKAVEALRRMGKKAVMINNNPETVSTDHDMSDSLYFEPLSEEDVLPVLKREN
                     VQGVFASFGGQTSLKAGLALEGEGVRILGSPSGVVRATEDRGHFSGLLERLGILQPLG
                     CSVKSVDEGLEKASMIGYPVMVRPSFVIGGLAMKVVSSPSELVSVLEIALQTDEDQSV
                     LIDRFLPGAEFEVDVLCDGREIVIPGIFEHLDPAGVHSGDSVAIFPDVSLSQARRRKI
                     EDISRKIAVELGVKGLLNIQFVLSGDEFYVIEANPRASRTVPIAVKLSGIPLVEMAVR
                     AALGENLSDMGLKPGLCERIRLFGVKVPVFSTEKLPGVDALPGPAMQSTGEALGVGDT
                     VAVALYEGLRGAGWRVPEKGRLLLSVADRSKLEAPHVAAAFHALGWSVDATSGTADVL
                     RNWGIPCRKVEKGKPLISGIASRQWDLIVNVASGSPEVLRDGYQMRRSAIEASIPCVN
                     DLHVASALAVSISLRIRGSHQ"
     gene            3529..4290
                     /locus_tag="PK146_03995"
     CDS             3529..4290
                     /locus_tag="PK146_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPE91435.1"
                     /translation="MKRHIVLALMLVVLAVFCPLIEAYGTPEGALRVGTESSFPPFVF
                     RDDSGGLEGFEIDLAKAVASELDREVIFIDMAFDALLPALSAGRIDMIAAGLSITPER
                     LKRAYFSKPYFFSRDAVVVRKGEAGPSDTDWFKGRRVAVQARTIQDDHLTSMGGVDII
                     RYQRSGEALRAVLEGKADAAFMDGVSAERFLVDDPSLGEGLKVALSGQITSGAMGLAV
                     RKIDGELLEKVNSALEALSAEGHLDQLREKWFGEI"
     gene            complement(4311..4802)
                     /locus_tag="PK146_04000"
     CDS             complement(4311..4802)
                     /locus_tag="PK146_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91436.1"
                     /translation="MTGRRTGAMIVGAFLLALALMTAAGALDEGLCLFPDITGWRGGE
                     TLWKELETLSEVMGYRSERLYRREADNHSIQVILLKGPGTLWQGLPKGEICADDGPVG
                     SGATFRTLEAGGYRAVMEDHPLTGKALVVEMAPDSTLTFETAFEDVDLERFALVFLKQ
                     YQP"
     gene            4896..>5443
                     /locus_tag="PK146_04005"
     CDS             4896..>5443
                     /locus_tag="PK146_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPE91437.1"
                     /translation="MYGFNISVLGCQMNVYDADRIRTALAAKGWEEVSEEDADVVVLV
                     TCSIRDKAEQKAWSDIGRLASRREPPLIAVVGCMAQRVGTLLMRRFPSVRLVCGPRSL
                     GKLPDALEKTMTSGKDIVLLDEDPLTLYDLPEVPLLRSNPWKAFITIAHGCDNFCTYC
                     IVPHVRGRFISRDPDEIFREVE"
BASE COUNT         1146 a         1446 c         1678 g         1173 t
ORIGIN      
        1 cactggcttc aggagcatca acgtacaaac tccccttcgg ccaccgaggg accaaccatg
       61 ctggtaaaga gtgcgcagga gggagagcct tcgtaacgag ccagaatcat gattacgcag
      121 ttcttgatga cagccttgag ggtacaggac tcgagataac tcaccgcaac ctgggtgacg
      181 ggagcgttga gggccttaag agcagagata tgagggtcgt cgctgtccag tatcaccctg
      241 aaggggctcc ggggccaagg gatgatgaca ggttttttga agaattcctg ttcatgtgcg
      301 atgggggggc gacgctatga aaactcccgg tggacctatc ctggtccttg gttccggtcc
      361 catacgcata gggcaggcag ccgagttcga ttactcagga agccaggcct gtcgtgccct
      421 cagggagatg gggtaccacg tcattcttct taacagcaac cccgccacca tacagaccga
      481 taccgccatg gccgatgtag tctacatcag gcccctcgaa gccgaagtgg tggagaggat
      541 attggcgacc caccgacctt ctggggtcat tgccactctg ggaggccagg tggccctgaa
      601 cctctgcgtc gaacttcaaa gaagagggct gtgggaaagg ttcggggttg aggtcctggg
      661 gacctcgccc cggtctgtcc agcgttcaga gggaagggag gattttcgtc aggccatgct
      721 gggcatctcg gagcctgtta tcgagagcat ctacgtggac aacgtcgaga gtgccctcca
      781 gttcgcctca gtgtcgtcgt ttcccttcat aatccgtcct gactataccc tcggtggcac
      841 cggcggaggt gtggctttcg accctgattc actggccgaa cgcgttgcag aaggactgag
      901 gctctctccg gtctcgaggg tgcttgtgga gcgctatctc caggggtgga gagagatcga
      961 gctcgaggtg ataagggacg gagcgggaaa caggttatgt gtatgtggaa tggagaacgt
     1021 ggaccctatg ggcattcaca ccggggacag tattgtagtt gccccggtgc ttaccctttc
     1081 ggacggacag tggcaacgcc tccgttccgc tgccttccgt attgtcgacg aacttgagat
     1141 catgggggct tgcaacgtac agttcgccct gtcccctgat gctggtgagt atgctgttat
     1201 tgaggtcaac ccccgggcaa gtcgctcgag tgcacttgcg agcaaggcta cagggtatcc
     1261 tatcgcccgg gttgctgcaa ggatagccgc cgggtccctt cttacggaga tgaccaaccc
     1321 cgtgacaggt gtgggaagcg ccctttctga accggcactg gactacgtgg tggtgaaatt
     1381 tcccagatgg cctttcgaga aattccccgg tgccgagaac agggttggaa caaggatgaa
     1441 gtccaccgga gaggtcatgg cccttggtcg cacattcatc gaggctttgt ccaaagcggt
     1501 caggtcgctg gacctcccgg gcgggatcgt ggaccccagg atgggtttgg caacaattga
     1561 ggatctggag gaggatcttt cgctaccgac tcacagaagg ctctggtcca tctttgaact
     1621 ccttggccgg ggtcttcccc cgcgggatat ttcggccagg tcagggatac accccttctt
     1681 tatcgaccaa cttgccagga tcgtatcggt tcttggggaa ctcaaggaaa gagagccgga
     1741 tgagactctt cttcggaagg tcaaatccat gggcctttca gacagggaca tagcctccgc
     1801 ttccggatcg gactggaggc ggatccggga cgcaaggctc tccctcggca tcgagagagg
     1861 gtacagggag gtcgacggca gtgccggcga gaccccggcg ggatccggtt actattacgg
     1921 cacatgcgga gcgtcagacg acgggatccg tcatgacagc agggagacct acgcggtcct
     1981 cggttccggc cccattagga tcggtcaggg ggtagagttc gactactgct gcgtaaaggc
     2041 agtagaagct ctcaggcgga tggggaaaaa agccgttatg ataaacaaca atcccgaaac
     2101 ggtcagcacg gaccacgaca tgtccgactc cctttatttt gagcccctct ctgaggagga
     2161 cgttctgcct gtactgaaaa gggagaatgt ccagggagtg ttcgcctcct tcggcggcca
     2221 aacgtccctg aaggcaggtc tagccctgga aggggagggg gtccggatcc tggggagtcc
     2281 ctccggtgtt gtccgggcca ccgaggacag ggggcatttt tccggtcttc tggagcgact
     2341 gggcatcctc cagcccctcg gctgttcggt aaagtccgtc gatgagggac tggaaaaggc
     2401 ctcaatgatt ggttacccgg ttatggtaag acccagcttt gtgattggcg gactggcgat
     2461 gaaggttgtg agctctccgt cggaactggt ctcggtcctg gagatcgcac tacagaccga
     2521 cgaggaccag tcggtcctga tcgaccggtt cctcccgggg gctgagttcg aggtggatgt
     2581 cctctgcgat ggaagggaga tagtcatccc gggaatattc gagcaccttg atcctgcggg
     2641 ggtccactcc ggggattcgg tggcgatatt ccccgacgtg tccctttccc aggcgagaag
     2701 aaggaagatt gaggatatct ccaggaagat cgctgtggaa ttgggagtaa aggggcttct
     2761 gaacattcag ttcgttctct ccggagatga gttctatgtg atagaggcca accctcgtgc
     2821 gagccgtact gttcccattg cagtgaaact gtcaggcata ccattggtgg agatggccgt
     2881 cagggctgcc ctgggggaaa atctgtctga tatggggctc aaacccggtc tctgtgaaag
     2941 gatacgccta ttcggggtca aggtacccgt cttttccacg gaaaaacttc caggagtgga
     3001 tgccctaccg ggtccagcga tgcagtctac cggagaagcc ctgggtgtgg gggatactgt
     3061 cgccgttgct ctttacgaag gtttgagggg agccgggtgg agagtaccgg aaaaggggag
     3121 acttcttctc tccgtcgccg acaggagcaa actggaggcg cctcacgttg cagccgcgtt
     3181 ccacgccctt ggatggagcg ttgatgcaac gtcagggact gctgatgtgc tcaggaactg
     3241 ggggatacca tgcaggaaag tggaaaaggg gaagcccctc atttccggga tcgcttcccg
     3301 gcaatgggat ctcatagtga acgtagcatc gggttccccg gaggtactca gggacgggta
     3361 ccagatgaga agatccgcca tagaggcatc gatcccctgt gttaacgatc tccacgtggc
     3421 atctgccctg gctgtctcca tctccttgag gataaggggt tcccatcagt gagggaggga
     3481 attataatct ccctttcagc gatccattac cggaggtagg attacaaaat gaaaagacac
     3541 attgttctgg cgttgatgct ggtggtcctt gcggtcttct gtcccctcat tgaggcttat
     3601 ggcacacctg agggtgccct cagggtgggg accgaatcct ctttcccccc atttgtcttc
     3661 cgtgacgatt caggaggtct ggaaggattc gagatagacc ttgcaaaggc cgtagcttcg
     3721 gagctggaca gagaggtcat cttcatcgat atggctttcg atgcgctcct ccccgccctc
     3781 tctgcaggga ggatagacat gatagctgcc ggtctgagca taactcccga gcgactgaag
     3841 agggcatact tctccaaacc ctatttcttt tcacgggatg ccgtcgtcgt caggaaggga
     3901 gaagcaggac cctctgacac ggactggttc aagggcagaa gagtggcagt ccaggccagg
     3961 acgatccagg acgatcacct gacatcaatg ggaggggtgg atatcatccg gtaccagagg
     4021 tccggagaag ctctcagggc agtcctcgaa gggaaggccg atgccgcatt catggacgga
     4081 gtatctgcgg agaggttcct cgtcgacgat ccttcgctgg gagaggggct caaggtggcc
     4141 ctatcaggtc agataacttc cggagcgatg ggccttgcag tgaggaagat agacggggag
     4201 cttctggaaa aggtcaactc tgcactggaa gctctttctg ccgaagggca cctggaccag
     4261 ctcagagaga agtggttcgg ggagatctga gggatcctgc caatgatcct tcacggctga
     4321 tactgtttca ggaagaccag ggcgaaccgc tcaaggtcga cgtcctcgaa ggctgtctca
     4381 aaggtcagcg ttgagtccgg ggccatctca accacgaggg cctttcccgt aagggggtga
     4441 tcctccatga cagccctgta cccccctgct tccagggtcc tgaaagtggc tcccgagccg
     4501 acgggaccat cgtcggcgca tatttctccc ttcgggagac cctgccagag agtgccgggc
     4561 cctttcagca ggatcacctg gatagagtgg ttatctgctt cccgacggta aagtctttca
     4621 cttcggtagc ccataacctc ggagagggtt tcgagctcct tccaaagggt ttcacctcct
     4681 ctccacccag tgatgtccgg gaacaggcag agtccctcat caagggctcc agccgcggtc
     4741 atgagagcca gagcaagcaa gaaggccccg acgatcatcg cccccgtcct tcttccggtc
     4801 attgcaaccc ccctctttct tgttacgggt tcgaaagtgg tatcattccg tggtgaagat
     4861 gatccttcca gattatttta catggaggcg acggcttgta cggtttcaat atcagtgttc
     4921 tcgggtgcca gatgaacgtc tacgacgctg acaggatcag gacggccctg gcggcgaaag
     4981 gatgggaaga ggtgtccgaa gaggatgccg atgtggtcgt ccttgtcact tgcagtatac
     5041 gtgacaaggc cgagcagaaa gcgtggagcg acatagggag actggcttca cgccgggagc
     5101 cccccctgat cgctgtcgtg ggttgcatgg cccagagagt gggtaccctt ttgatgagac
     5161 gcttcccctc ggtacgtctt gtatgcggac cacgcagcct tgggaagctg cctgatgcac
     5221 tggagaagac catgacctcc ggaaaggaca ttgtcctcct cgatgaggac cccttgaccc
     5281 tttatgacct tcccgaggtg ccactactcc gctcaaatcc ctggaaggcc ttcatcacca
     5341 tcgctcacgg atgcgacaac ttctgtacct actgcatcgt gccccatgtc aggggaaggt
     5401 tcatctccag ggacccagac gagatattcc gtgaggtaga gag
//
LOCUS       DAOPKQ010000082         5442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_16217_length_5442_cov_4.360126, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000082 DAOPKQ010000000
VERSION     DAOPKQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16217_length_5442_cov_4.360126"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..2688)
                     /gene="secA"
                     /locus_tag="PK146_04010"
     CDS             complement(25..2688)
                     /gene="secA"
                     /locus_tag="PK146_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPE91438.1"
                     /translation="MLEALKKSLGLDRNERTLKRFASAVSRINALEPSMQALSDDDLA
                     DLGPLFRERAIGGESLDELLPEVFAAVREVSGRTLGLRHFDVQLMGGIALHEGKIAEM
                     KTGEGKTLVATLAVVLNALKGEGVHLVTVNDYLAQRDAEWMGPIYKFLGLSVGVIYAF
                     MDQAERVAAYKADVTYGTNSEFGFDYLRDNMAVSASQLVQREHSFIILDEVDSILIDE
                     ARTPLIISGPSEDNVEVYRKADDVARRLRKGEDFELEEKERNVALTEAGIARCEKVLG
                     LPDLFTDYQRSELGHRIVQALKAHHLFQRDVHYVNKDGEIVIVDEFTGRLMFGRRYSD
                     GLHQAIEAKERVSVGRENQTLATITLQNYVRMYRKVAGMTGTAATEEEEFKDIYGLEV
                     VVIPTHMPMIRKDNPDVIFRTRQEKFAAVADDVEETHRTGQPVLIGTTSIESSEILGK
                     ILKSRRIEHRILNAKYHEMEAHIVAQAGRLGAVTVATNMAGRGTDIVLGGNPLFLARE
                     EAQRREVDHASDRETFEEILSEMREECSREHGEVVRLGGLKIIGTERHESRRIDNQLR
                     GRSGRQGDPGCSTFYLSLEDDLLRLFGSDRISGFMEKLGLEEGEYIEHGLLTKAIETA
                     QKRVEEFHFDIRRQLLMYDNVMNQQREAIYQERRKILSEPDLAGHGRQLVEDALESII
                     ERFFPEGEEPQPQAVAVSLKGVFWPGIERHLEGVQTPEDLEVSRAAIMEEVSRRLVQK
                     LEEMGALNASEMIRFLLLNVLDSAWKEHLLAMDELRRGIGLRAIGQKDPLLEYQFESY
                     NLFQEMMERVRLSFAQYFFRVSVMSEGSAPARHRDALKETRDIALPVPDGQGTGPARP
                     QPVRSGGKIGRNAPCPCGSGRKYKHCCGKNV"
     gene            complement(2809..4206)
                     /locus_tag="PK146_04015"
     CDS             complement(2809..4206)
                     /locus_tag="PK146_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPE91439.1"
                     /translation="MVRKIQPEPFRIRMVEPVTVPDQAQREDALRKGHFNMFGLTSRD
                     IYIDLLTDSGTGAMSKQQWAALMRGYEAYAGADSFFALKDSVREVLGFEYVIPAHQGR
                     AAENIAFGTLLKKGDIVPFNMPFDTTRAHIFNMGADPVDCVVDDAFDPSSDHPFKGDV
                     DIRKLEDTIRHYGKDRIPLIMVTVTNNSGGGQPVSLGNLRSVSAVAKREGIPFFLDAA
                     RMAENAHFIKVREKECADMSVAQILRAMMDCVDAITVSCKKDPLVNIGGMICCRTEDM
                     YQKLLPRVILYEGFVTYGGLAGRDLEALALGLREMTDEQYLAHRVAQVAYLGDILEEG
                     GVPYVKPAGGHAIFIDAAAFLPHIPQKHYPADVLGIEIYREAAIRGIGLGALAFATED
                     EKTGEMMLPRLELFRLAINRRTYTNSHIEYVGESIVNVFKRRDSIRYGLKVTYAPPVK
                     GLHHFLAHLEPFDLK"
     gene            complement(4354..4896)
                     /gene="pgsA"
                     /locus_tag="PK146_04020"
     CDS             complement(4354..4896)
                     /gene="pgsA"
                     /locus_tag="PK146_04020"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPE91440.1"
                     /translation="MTVWNLPNILSLSRVFLAPLVMVFLTLRGQFGNILGLPFGDVLA
                     GLVFTIAALTDTFDGYFARKHGMVTNLGKLIDPLADKILVIAALISLVQLQRLPAWIV
                     VVIISREFFVTGLRMVAASEGVVIAASRGGKVKTVTQIVAILLVIFNLPGGLAAMWVA
                     MIATLWSGMDYLVEGRKLLT"
     gene            complement(4957..>5442)
                     /locus_tag="PK146_04025"
     CDS             complement(4957..>5442)
                     /locus_tag="PK146_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91441.1"
                     /translation="VRRGIVMCPEGRRILPHLTVFENLMLGAYTRNDKKAIRKDLDWV
                     YELFPRMKERTWQKGGTLSGGEQQMLAVGRAIMSAPEVIMLDEPSLGLAPLLVKEVFD
                     IIQTINSEGKTVLLVEQNAYAALKIADAAYVLEVGRIVLSGTGKELLDDPKVREAYLG
                     G"
BASE COUNT         1075 a         1633 c         1471 g         1263 t
ORIGIN      
        1 ggaacaggac ggtagattcg gttctcagac attctttccg caacagtgct tgtattttct
       61 cccgctaccg caggggcagg gtgcgttcct cccgatcttg ccgccgctcc tgacaggctg
      121 gggcctggcg ggacctgttc cctgaccgtc gggaacaggc agtgcaatat ccctggtctc
      181 tttcagggca tcccggtgcc tggcaggggc cgacccctcg gacatcacgg aaaccctgaa
      241 aaaatattgt gcgaaggaga ggcgtaccct ctccatcatc tcctggaaaa gattatagga
      301 ttcgaactga tattcaagaa ggggatcttt ctgccctatt gccctcagcc ctatccctct
      361 ccgcagttcg tccatggcca gaaggtgctc cttccaggcc gaatcaagaa cgttgaggag
      421 caggaacctg atcatctcgc tggcgttgag ggcacccatt tcttccagct tctggacgag
      481 cctccgggag acctcttcca ttatggcagc ccttgatacc tccaggtcct ccggggtctg
      541 aaccccttca aggtgcctct ctatacccgg ccagaagaca cccttaagag agacagccac
      601 agcctggggc tggggttcct ccccttcggg gaagaagcgc tcgatgatgc tttcaagggc
      661 atcttcaacc agttgacggc catgcccggc aaggtccggc tcggagagga tcttcctgcg
      721 ctcctgataa atggcctccc tctgctggtt catcacgtta tcatacataa gaagctggcg
      781 acggatgtca aagtggaact cctccacccg cttctgagcc gtctctatgg cttttgtgag
      841 aagaccatgc tctatgtatt caccctcctc gagccccagc ttttccatga acccgcttat
      901 ccggtcggag ccgaaaaggc ggagaagatc atcctcgaga gagaggtaga aggtgctgca
      961 cccggggtct ccctggcgtc cactcctccc ccggagctgg ttgtctatcc ttcgtgactc
     1021 gtgccgttcg gttccgatga tcttgagacc accgagcctg accacctcgc cgtgctccct
     1081 ggaacactcc tccctcattt cggagaggat ctcctcgaag gtttccctgt cggaagcatg
     1141 gtcaacttct cttctctggg cctcctccct ggcgagaaag agagggttgc caccgaggac
     1201 tatgtccgtt ccacgacccg ccatgttggt cgcaacggtg accgccccaa gccttccggc
     1261 ctgggcgact atatgggctt ccatttcatg gtacttggcg ttcaggatcc tgtgttcgat
     1321 ccttcttgac ttcagtatct ttccaaggat ctcggaactc tcgatggaag tcgtacctat
     1381 caggacgggc tgacccgtac ggtgggtctc ttccacatca tcggccacgg ctgcgaactt
     1441 ctcctgcctg gtccggaata tcacgtcggg gttgtccttc ctgatcatag gcatatgggt
     1501 gggaatgacg accacttcca gaccgtatat gtccttgaac tcctcctcct cggtagcggc
     1561 ggtccccgtc attccagcca ccttcctgta catcctgacg tagttctgaa gagtgatggt
     1621 ggccagggtc tggttctccc ttcccacgct gaccctctcc ttggcctcga tcgcctggtg
     1681 aagcccgtcg gagtaccttc ttccaaacat cagccttcct gtgaattcgt cgacgatgac
     1741 tatctcgcca tccttgttta cgtaatggac atctctctgg aagaggtggt gggccttgag
     1801 agcctggaca atcctgtgcc ccagttcgga tctctggtag tcggtgaaca ggtcgggcag
     1861 accaagtacc ttctcgcacc tggcgatccc cgcctcggta agggccacgt tccgctcctt
     1921 ctcttcgagt tcgaagtcct cacccttgcg aagcctcctg gctacgtcgt cagcctttct
     1981 gtagacctcc acgttgtcct cggaggggcc agagatgatc aggggggtcc ttgcctcatc
     2041 gataagaatg gagtccacct cgtcgaggat tatgaaagag tgctcacgct gaacaagctg
     2101 ggaggcgctt acggccatgt tgtccctgag gtagtcgaaa ccgaactcgc tgttggtacc
     2161 gtaagtgacg tccgccttgt aggccgctac tctctcggcc tggtccatga aagcgtagat
     2221 cactcccacg gagagtccca gaaatttata tatgggcccc atccactctg cgtccctttg
     2281 agccaggtag tcgttgacgg tcaccaggtg caccccttcg cccttcagcg cgttaaggac
     2341 cacagcaagg gttgccacca gggtcttgcc ctcgccggtc ttcatctcgg ctatcttgcc
     2401 ctcgtggagc gctattcccc ccatgagctg gacatcaaag tgccggagtc ccagggtcct
     2461 ccctgacact tcacggacgg cggcaaacac ttcgggtagc agttcgtcca gggactcgcc
     2521 gccaattgct cgttccctga aaaggggtcc caggtcggca agatcatcat ccgagagggc
     2581 ctgcattgac ggttccaggg cgttgatcct cgaaaccgcg gaagcgaagc gtttcagggt
     2641 cctttcgttc cggtcgagcc ccagagactt tttcagtgct tctaacattc agttcacctc
     2701 gatcggcatc agggacgggg agttttcctt tcagagtgac attatattcc ccgcagggag
     2761 gggcgtcaaa aaaggggggg cggccaggcc gtcccccccg aggggtcgtc acttcagatc
     2821 gaaaggctca aggtgggcca ggaagtggtg aagccccttg acgggtggtg cgtaggtgac
     2881 cttcagaccg tagcgtatgc tgtccctgcg cttgaagaca ttcacaatgg actcgcctac
     2941 atactcgatg tggctgttgg tataggtcct ccggttgatg gccagacgaa agagctcaag
     3001 cctgggaagc atcatctctc cggtcttctc gtcctctgtg gcaaaggcca gggctccgag
     3061 tccgatcccc cttatggccg cctcacggta tatctcgatg cccagcacat ccgccgggta
     3121 gtgtttctgt gggatgtggg ggaggaatgc cgcggcgtcg ataaagatgg cgtgtccccc
     3181 tgccggttta acgtagggga ccccgccctc ctccaggata tcgccgagat aggccacctg
     3241 ggccacacgg tgtgccaggt actgctcgtc ggtcatctcc cgaagaccga gggccagagc
     3301 ctccaggtcc ctgcccgcca gcccgccgta ggtgacaaaa ccttcgtaga ggatgacccg
     3361 agggaggagc ttctggtaca tatcctcggt cctgcagcat atcatccctc ctatgttgac
     3421 cagggggtcc ttcttgcagg atacggttat ggcgtcgacg cagtccatca tggccctcag
     3481 gatctgcgcc acggacatat cggcgcactc cttctccctt accttgataa agtgggcatt
     3541 ctccgccatt cttgcagcat caaggaagaa ggggatccct tccctcttcg caacggccga
     3601 aacggatctc aggttaccca ggcttaccgg ctggcctcca ccggagttgt tggtgacggt
     3661 caccattatc aggggtatcc tgtccttgcc gtaatgcctg atagtgtcct caagcttccg
     3721 gatgtccaca tcacccttga aggggtgatc agaggaagga tcgaaggcgt cgtcaacgac
     3781 gcagtcgacg ggatcggcac ccatgttgaa gatatgggcc cgggtagtgt caaagggcat
     3841 gttgaagggt acgatgtcgc ctttcttcag gagggttccg aaggcgatgt tctcggcagc
     3901 cctcccctgg tgggccggaa tgacgtattc gaagcccaga acttcacgga cggaatcctt
     3961 cagggcaaag aagctgtcgg cccctgcata ggcttcataa cctctcatca gagccgccca
     4021 ctgctgcttg ctcattgccc ctgtgccaga gtccgtcagc agatcgatgt agatgtcacg
     4081 ggaagtaagg ccgaacatat tgaaatggcc cttcctgagg gcgtcctccc gctgtgcctg
     4141 gtccggtacc gtgacaggtt ccaccatcct gatacggaaa ggttcaggct gtatcttcct
     4201 aaccatctag caagacctcc tacttctttt cttcggctcg gctgaaggtg cctctcgtgg
     4261 tgccaggctc gtctttcgct tcagttaatt atagcaccct tcatccgacc attttgaccc
     4321 ggggggacag ggtccatttc cctataccgg gcttcaggta aggagttttc taccctcgac
     4381 caggtagtcc atacccgacc agagggtggc tatcatggcg acccacatgg cggccagtcc
     4441 acctggaagg ttgaagatca ccaggaggat cgccacgatc tgggtaacgg tcttcacctt
     4501 gcctcccctg gatgctgcga tgacaacccc ttccgaggcg gcgaccatcc gaagaccggt
     4561 gacgaagaac tccctggaga tgatgaccac aacgatccag gccggcaatc gctgaagctg
     4621 cacaagagaa ataagggctg cgatcaccag gatcttgtcg gcaaggggat cgatgagctt
     4681 gccgagattg gtcaccattc cgtgcttccg ggcgaagtac ccgtcaaagg tatcggtgag
     4741 ggccgctatg gtgaatacaa gtcccgcgag aacgtctccg aagggaagtc cgagaatgtt
     4801 cccgaactgc cccctcaggg taaggaatac catgacgaga ggggcaagaa atacccttga
     4861 gaggctcagg atgttgggaa ggttccatac ggtcaaggct catacacctc cagtaccgcg
     4921 taaggggccc tgtcttattt cagggccccc gttttcctac ccccccaggt aggcttcccg
     4981 gacctttgga tcatcaagca gttcttttcc tgtcccggag agaactatcc tgcccacctc
     5041 caggacgtat gccgcgtcgg ctatcttcag ggcggcatag gcgttctgtt ccaccaggag
     5101 gaccgtcttg ccctcgctgt tgatggtctg gatgatatcg aagacctcct tgacaagaag
     5161 gggcgccaga cccagagaag gttcatccag cattatcact tcgggggcac tcattatggc
     5221 acgaccgact gcaagcatct gctgttctcc tccgctaagg gtcccgcctt tctgccaggt
     5281 tcgttccttc attcttggga aaagctcata aacccagtcc agatccttcc tgatggcttt
     5341 tttgtcattt cttgtatagg caccaagcat caggttttca aagactgtaa gatgaggaag
     5401 gatccttctt ccttctggac acattactat tcctctacgg ac
//
LOCUS       DAOPKQ010000083         5335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_16678_length_5335_cov_4.757955, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000083 DAOPKQ010000000
VERSION     DAOPKQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16678_length_5335_cov_4.757955"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK146_04030"
     CDS             complement(<1..721)
                     /locus_tag="PK146_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPE91442.1"
                     /translation="MRTRTAVVVLVLSFFILASPFHCEAGTDELEKELKLGDKVSEQV
                     EKHWDRVTDPVRVARVSMVLSRLTPFTERDLPFEVRIVEEEMPNAFALPGGRIYLTTG
                     MLDFCRSDDELASILAHELVHTDRKHGLIQSSRNQRLTIGALAVIIATGGAGAAPILA
                     NLAQVAIMNSYSKDLEREADTSGLDIIFQSGYEPSGAVTVMERLQEEEMKRPYVDPGI
                     YAGHPKTRERVLSLVGIIRERG"
     gene            complement(850..1308)
                     /locus_tag="PK146_04035"
     CDS             complement(850..1308)
                     /locus_tag="PK146_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91443.1"
                     /translation="MFVCSGNTCRSPMAEGFMNHFLKIHGLDGIWGASSSGTMAIDGL
                     PANGLAVRVMSENGIDISRHRSKPAGSWEPPEDSLFLGMTRDHVNCLRNLFPRRRGAV
                     CLLGEASRPFYEGDILEVPDPFGGSLASYREIAGVIREMTMGLLNSLRDH"
     gene            complement(1343..2275)
                     /locus_tag="PK146_04040"
     CDS             complement(1343..2275)
                     /locus_tag="PK146_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HPE91444.1"
                     /translation="MIVTVTLNPAVDEEFLVPEFRPGKWFRTSHVDRSPGGKGINVSY
                     MLSQVGYPSVAMGFLAGFNGEYIRDALRRLRITTNFIHVPGETRSNVYIVDEVGHLET
                     GIAESGPYIPEEALGRFLSNYERMLGRARFVMIGGSLPPGVPQDIYRDLVDRAKSKGV
                     PTLLDAAGPPLMAAIEGGPAFARIDHRFMARVAGVSLTSLDNLIEVVSRVHDYGVEWA
                     VVSYRTYGAVFFSPQGIYLAEYERKGLVSLFAADDALVAGFMIAREERMDAEETIRFS
                     TACAWEDSLHVEKGFRDRSEIDRLASKVNIEKLD"
     gene            complement(2335..3522)
                     /gene="trxB"
                     /locus_tag="PK146_04045"
     CDS             complement(2335..3522)
                     /gene="trxB"
                     /locus_tag="PK146_04045"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPE91445.1"
                     /translation="MEKRELVIVGAGPAGLTAAIYGKRSGLDVLVLEKGIPGGQINVT
                     DEIENWTGVIHASGAELADTFRKHAEHFKAEFRETDVKGITVVGGRKVIHTDGDEIEA
                     EAVIIATGASFKKLGAPGEKEFTGRGVSYCAVCDGAFFEDQEIAVIGGGNTAVEEGVY
                     LTQFASKVTIIHRRDSFRADRLAIERAMSNPKIEVIWNTVVEEISGEDMVEKLVLKNV
                     RTGETSDLPVSGVFVFAGMDPNISFMQGLVDSVGSGWIVTNERMETNVEGIFAAGDVR
                     DKYLRQVITAAGDGATAAMAASAYISEQLHIQSLLMEPEQVTVFIFSSIDEPQARLAS
                     EIEKYSSGRSSKVVLLDGYKNRRVVEKLGITELPSLVEFRKGSIKRKQQAGSLEEAAK
                     FLS"
     gene            complement(3579..4427)
                     /gene="prmC"
                     /locus_tag="PK146_04050"
     CDS             complement(3579..4427)
                     /gene="prmC"
                     /locus_tag="PK146_04050"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPE91446.1"
                     /translation="MNLREVRKLASDKLHEAGIDRPWFEADLLICSITGMERILLLAH
                     PDRPFPEHLIPTLKAAIERRAAREPLQYITGVCEFMGRAFRVGPGCLVPRPETELLVA
                     ESRKHFKGGVFLDWGTGSGCIAASILLDNPASRCVAVDKCPVAITWAWKNLREKNLLG
                     RCLLCHCPSPERIPVPPPGFEMIVSNPPYIPSAQIPDLMPEVERYEPRSALDGGEDGF
                     DHYRSLAGWAPSALVPGGVLILEAGSDEQAERLKSVRTGELTPVSIVRDLQGTARVVV
                     LKRSPF"
     gene            complement(4431..>5335)
                     /gene="prfA"
                     /locus_tag="PK146_04055"
     CDS             complement(4431..>5335)
                     /gene="prfA"
                     /locus_tag="PK146_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPE91447.1"
                     /translation="SRLEEARGLIHSGDPELSDLARDEMHELEPQLEELESRLRLLML
                     PRDPNDDKSVIVEIRAGTGGDEAALFAGDLFRMYSLFAENNGWKVEVMNSNETGIGGY
                     KEVVFKVDGPGAYSQLKFESGVHRVQRVPVTESSGRIHTSTATVAVLPEAEDVDIDIR
                     QEDLKIDTYRASGAGGQHVNMTDSAVRITHLPTGLVVTCQDERSQIKNRVKAMNFLKA
                     KLYDMELREQQAKVAAERKGQVGTGDRSERIRTYNYPQNRLTDHRIGLTLYRLESMLE
                     GDLQEMISALIMADQSEKMRSLEG"
BASE COUNT         1014 a         1690 c         1440 g         1191 t
ORIGIN      
        1 agcccctctc cctgataatg cccacaagac tcaggaccct ttcgcgggtc ttggggtgac
       61 cagcgtatat tcccgggtcg acatatggcc tcttcatctc ctcctcctgg agcctctcca
      121 tgacggttac cgcccccgac ggttcatacc ccgactggaa gataatatcc aggccactcg
      181 tatcggcctc cctttcaagg tccttgctgt aggagttcat tatggctacc tgggcgaggt
      241 ttgcaaggat cggcgctgct ccggcccctc cggtggctat tatcaccgca agggccccaa
      301 tggtaagccg ctggttcctt gacgactgga tcagcccgtg tttcctgtcg gtatggacca
      361 gctcatgggc caggatcgaa gcgagttcat cgtcgctgcg gcagaaatcg agcatccccg
      421 ttgtgaggta gatcctcccg ccggggagcg caaaggcatt gggcatctct tcctccacga
      481 tcctgacctc gaaggggagg tctctctccg tgaagggggt gagtctcgaa agtaccatgg
      541 agacccttgc cacccggacc gggtcggtca cacggtccca gtgcttctca acctgttcgg
      601 agaccttgtc tcccagtttt aactctttct ccagttcatc cgtaccggct tcgcaatgga
      661 atggggaagc caggatgaaa aaggagagga cgaggacaac tacggcggtc cttgttcgca
      721 taggctatcc ttccgggacc gatgatggga tcctgtacct gatattctat ggcaaagacc
      781 cgttcacggg aatgtccccc aagaagtcgg ggcgaatggc cgccggtcgc ccgctgggat
      841 ttcggggtgt cagtgatccc tgagggagtt cagcaacccc atggtcatct cacggatgac
      901 cccggcaatc tcccggtaac tggccaggct gcctccaaag gggtctggaa cttcgagtat
      961 gtccccctcg tagaacggac gggaggcctc gccaagtaga cagaccgccc cccgccgtcg
     1021 ggggaacagg ttcctaaggc agttcacatg atctctcgtc attcccagga acagggaatc
     1081 ttccggtggt tcccatgaac cggcaggctt cgagcggtgc cgggagatat caattccatt
     1141 ctcggacatt acccttaccg caagtccatt ggcgggaaga ccatctatgg ccatggtccc
     1201 cgacgatgag gcgccccaaa taccgtccag gccatggatc ttgaggaaat ggttcatgaa
     1261 cccctccgcc attggactcc tgcaggtatt gcccgagcaa acgaacatca tcaccttgag
     1321 gggatccttg ttaaccatgt gatcagtcca gtttttcgat gttcaccttt gaggcgagcc
     1381 tgtctatctc tgacctgtcc cggaagccct tctccacatg gagcgagtcc tcccaggcgc
     1441 aggccgttga gaaccggatg gtctcctcgg catccatcct ctcctccctg gcgatcatga
     1501 aaccggcaac cagggcgtcg tcggcggcga agagtgagac caggccctta cgttcgtact
     1561 ccgccagata gataccctgg ggggagaaaa agaccgctcc gtaagtcctg taggagacaa
     1621 ctgcccactc taccccgtaa tcatgtaccc tggagaccac ctctatgagg ttgtccaggg
     1681 aagtaaggga cacccctgcg accctggcca tgaaacggtg gtctatcctg gcaaaggcgg
     1741 gacctccctc aatggctgcc atcaacggcg gccccgccgc gtccagcagg gtcgggaccc
     1801 cctttgactt tgccctgtcg accaggtcgc ggtagatatc ctgggggacc cccggcggca
     1861 gggagcctcc gatcatgacg aaccttgccc tgcctagcat cctttcgtag ttggagagga
     1921 atctccccag ggcttcctct ggtatgtagg gtccgctttc ggctattccc gtctcgaggt
     1981 ggccgacctc gtccacaatg tacacgttgg aacgggtctc gccggggacg tggatgaagt
     2041 tggtggttat gcgcagcctc ctcagggcat cccggatgta ctcaccgttg aaccctgcaa
     2101 ggaaacccat agctacagag ggatacccca cctgggagag catgtacgaa acgtttattc
     2161 ctttcccccc gggcgacctg tcaacgtggc tcgtccggaa ccatttcccc ggtctgaact
     2221 ccgggacaag gaactcctcg tccacggcag gattaagagt tactgttact atcatggacc
     2281 gcacctcccc tgagtatcag acaaacatga cgacgcgcac cggggcggcc ccgatcacga
     2341 taggaatttc gcagcctctt ccagggatcc tgcctgttgc ttccgtttga tgctcccctt
     2401 tctgaactcg accaaggatg gaagctctgt gatcccgagc ttctcgacca ccctccggtt
     2461 cttgtagccg tcaagaagga ccacctttga cgacctcccg gaagagtatt tctctatctc
     2521 cgatgccagc cttgcctggg gttcgtcgat gcttgagaag atgaagaccg tgacctgttc
     2581 gggctccatg agaaggctct ggatatgtag ctgctccgat atataggcag atgcagccat
     2641 ggctgccgtg gctccatcac cggcagcggt aattacctgc ctaaggtact tgtccctgac
     2701 gtcaccggct gcgaatatgc cctctacgtt ggtctccatc ctctcgttgg tcacgatcca
     2761 tccgcttccg acggaatcaa caagcccctg catgaaggag atgttggggt ccataccggc
     2821 gaagacgaaa acgcccgata cgggaagatc ggatgtctcc cctgtcctga cgttcttcag
     2881 aaccagcttc tccaccatgt cctctccgga gatctcctcg acgacggtgt tccatatgac
     2941 ctctatcttc gggtttgaca tggccctctc tatggccagc ctgtcggcac ggaagctgtc
     3001 ccttctgtgg attatggtca ccttcgaggc gaactgcgtc aggtagaccc cctcttcgac
     3061 ggccgtattg cccccgccga tgacggcgat ctcctggtcc tcgaaaaagg ccccgtcgca
     3121 gaccgcacag tagctgaccc ccctgccggt gaactccttc tctcccggag cccccagttt
     3181 cttgaaactt gccccggtgg caatgatcac ggcctcggcc tcgatctcat caccatcggt
     3241 atgaatgacc ttccttcctc cgacgacagt aatacccttt acatcggtct cacggaattc
     3301 agccttgaag tgctcggcgt gctttcggaa cgtatcggcc agctctgctc cgctggcatg
     3361 gatcacccct gtccagttct ctatctcgtc ggtgacattt atctgccctc ccgggatccc
     3421 cttttcaagg acaagaacgt caagacccga tctcttcccg tagatggcgg ccgtgagccc
     3481 tgccggccct gctccgacaa taaccagttc cctcttttcc atcgatggat ccctcctggg
     3541 attaatgttt tctttgcatg aaagcaaaca tcattgtatc agaacggaga cctcttcaaa
     3601 actaccaccc tggccgtccc ctgcaggtcc ctcactatcg acaccggggt cagctcccct
     3661 gtccggacgg acttcagcct ctccgcctgt tcatcgctcc ccgcctcgag gatcagcaca
     3721 cctccaggca caagggccga cggggcccat ccggcaagag acctgtaatg gtcaaagccg
     3781 tcctctcccc cgtcgagggc ggagcggggc tcataacgtt ccacctccgg catcaggtcg
     3841 gggatctggg cagagggaat gtacggggga ttgctgacta tcatctcgaa ccccgggggc
     3901 ggaacgggga tcctctctgg ggaagggcag tggcagagaa gacatctccc aagaaggttc
     3961 ttctccctga ggttcttcca ggcccaggtt attgcgacgg gacatttatc gacggcaacg
     4021 cacctgcttg cggggttgtc caggagtatc gatgcggcaa tgcacccgga accggttccc
     4081 cagtccagga agaccccccc cttgaaatgc ttccggctct cggccaccag aagctcagtc
     4141 tcgggacggg ggacgaggca ccccggtcct acgcgaaatg ctctgcccat gaattcacat
     4201 acgccggtta tatactgcag cggttccctg gcggcccttc tctctatggc cgccttcagt
     4261 gtcgggataa ggtgttccgg gaagggtcta tccgggtggg cgaggagaag gatgcgttcc
     4321 atgccggtaa tggagcagat cagcaggtct gcctcgaacc agggcctgtc gatcccggcc
     4381 tcatgaagtt tgtccgaggc gagtttgcgg acctcccgaa ggttcaaggc tcagccttcc
     4441 agggatctca tcttctccga ctgatcggcc atgatcaggg cggagatcat ctcctggaga
     4501 tccccctcga gcatgctttc caacctgtat agtgtcagtc cgatacggtg gtcggtcaac
     4561 cggttctggg ggtagttgta ggtgcgtatc ctctccgacc ggtctccagt ccccacctgc
     4621 ccctttcgct ccgcagccac cttcgcctgc tgctccctta gctccatgtc atacagcttg
     4681 gccttcagga agttcatggc cttgacgcgg ttcttgatct gggatctctc atcctggcag
     4741 gtcacgacaa ggcccgtggg gaggtgggtt atccggacgg cggaatcggt catgttgaca
     4801 tgctgcccgc cggccccgct ggcccggtag gtatctatct tgaggtcctc ctgcctgatg
     4861 tcgatatcca catcctcggc ttcgggaagc actgctaccg tcgcggtgga tgtatggatc
     4921 cttccgcttg attcggtcac ggggaccctc tggaccctgt ggacgccgct ctcgaatttg
     4981 agctggctgt atgcacccgg gccgtcgacc ttgaacacta cctccttgta ccctcctata
     5041 cccgtctcgt tgctgttcat gacctccact ttccagccat tgttctcagc aaagagggag
     5101 tacatgcgga agaggtcccc cgcaaagaga gccgcctcat cccctccggt ccctgccctg
     5161 atctcaacga taacgctctt gtcgtcgttg ggatccctcg gcagcatgag gagccgcagt
     5221 ctggactcca gctcctccag ctgcggctcc agttcgtgca tctcatccct ggccaggtct
     5281 gagagctcgg ggtctccgga gtgaatgaga ccccttgcct cttcgagcct ggaga
//
LOCUS       DAOPKQ010000084         5332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_16687_length_5332_cov_4.086792, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000084 DAOPKQ010000000
VERSION     DAOPKQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16687_length_5332_cov_4.086792"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PK146_04060"
     CDS             <1..445
                     /locus_tag="PK146_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE91448.1"
                     /translation="MTGGQSHPGIGDKLRKGETGRRVDLESAVKGCGVNWVKTTEAYD
                     ISTSRTLVKEAWDYARTHEEPAVVIFRHPCMLLRQKQAVIPVTVDPEKCIGCKFCINF
                     FNCPGLVFDEKSKKAYIDERFCVKCGVCINVCPHGAILATEGEEA"
     gene            448..1005
                     /locus_tag="PK146_04065"
     CDS             448..1005
                     /locus_tag="PK146_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE91449.1"
                     /translation="MQYVIVGIGGQGILFSSRVLGHIALSRGERVVGSEVHGMAQRGG
                     SVISHFKVGDYSSPMVKAKEADVLLAFDQNEAVRNLHFLKQGGHAIINTHEPAFIENP
                     SLRKWMSDRGVKVHTVMGYDILKREMGGRFLFLNVLILGAMCGAGVGNVTIEEVREAV
                     RVLAPAKFVEDNVKAVDLGHAGASR"
     gene            986..1531
                     /locus_tag="PK146_04070"
     CDS             986..1531
                     /locus_tag="PK146_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91450.1"
                     /translation="MREPVDDRITGSKRGAGNGLPAYRAPRARKAYTLVETAVVLSIV
                     IAISFTVSPIVTGLISQRAGSELAARAEARNMARWIERTLQKACLHRRAFNVKYLSGF
                     RQEFLILWFDPPEYERYQTNGRCLVYFKSTSTQDRCYTPAWHTMTPAFTLYVYTPGRS
                     KKKIAEIAVSGYCSVRLTEFP"
     gene            1566..2087
                     /gene="lepB"
                     /locus_tag="PK146_04075"
     CDS             1566..2087
                     /gene="lepB"
                     /locus_tag="PK146_04075"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPE91451.1"
                     /translation="MVAVKPWWRETIETVLWALVLALVLRALVIQAFWIPSGSMIPTL
                     LPRDRVLVAKFWYSFAEPKRGQIIVFKYPLDPKRDFVKRLIGLPGETVEIVEGTVMID
                     GEPLQEPYVKNRDSLSFGPLEVPEGNYFMLGDNRPNSQDSRFWGFVPEANIKGPAFIR
                     YWPIPRVGGLYKE"
     gene            2084..2923
                     /locus_tag="PK146_04080"
     CDS             2084..2923
                     /locus_tag="PK146_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPE91452.1"
                     /translation="MTRTVWYPGHMARGLRKLRELSEKLDLLVEVRDARAPALTGSPF
                     SEQVARFRPVWIVLSKKDLAVEEATARWLSFFRDQGGKAWALDLRKGNLEPLLRTAAR
                     LRPSHRELRLAVIGIPNVGKSQLLNGIIGKNTAPVGGIPGITRGVSWYKGKGFLVVDS
                     PGIVDPRSHREVHRRLAWLGSSRSDVIGGYEDLAEGLVRFLGGSSMLERTLSSWEMER
                     LDETPSDTLRRIGVRLGCLVKGGEVNMDLAGKRFIEAFSTGRIGRFTLEEPGDRRPWE
                     DEP"
     gene            2920..3489
                     /locus_tag="PK146_04085"
     CDS             2920..3489
                     /locus_tag="PK146_04085"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPE91453.1"
                     /translation="MIVAGVDEAGRGPLAGPVVAAAVILDRGETARLLSMGLKDSKRL
                     SSSSREAIFKEMKRMGVAWRAQAASPGRIDRDNILQATLWAMNRSVTMLPREPGLVVV
                     DGSTPIPGLGVPQRALPGADGMVPAVAAASVVAKVLRDRAMCSLDSIFPCYGFRRHKG
                     YASREHVEALRREGPSRIHRTSFQVRGWT"
     gene            3563..3934
                     /locus_tag="PK146_04090"
     CDS             3563..3934
                     /locus_tag="PK146_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPE91454.1"
                     /translation="MAKHIDLGAAGEAMAARELLKLGYRLIDTNVRMKMGELDIVAEE
                     GGEMVVVEVRTRTLGRMVPPEATVGPSKIRKLVRTGRTYMESVGWEGPWRIDIAAITV
                     GSDGSPKLEVFRDITVGIIEP"
     gene            3931..>5332
                     /locus_tag="PK146_04095"
     CDS             3931..>5332
                     /locus_tag="PK146_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPE91455.1"
                     /translation="MNVVHGVTLQGIKALKVEVEVEITGGLFVIAVVGLPDASVKEAR
                     ERVRAALRSLGVSVKGRVAVNLAPADSPKEGALLDLPIAVGIAHAKGEIRVPGPTLFM
                     GELALDGRVRRVRGAVPAALLARDLGFPLFVPEGNAREVSLVKGVRAWQVGHIRELFA
                     HLRGQSQLKVIEQAEEATGDPEPDPDFSDIKGQVAAKRALEIAAAGHHNVLLTGSPGS
                     GKTLLARALKDILPPLSDEEMMEVLLVRSTVGLPLDSSRRRPFRAIHHTSSTISVCGG
                     GTNLRPGEVSLAHRGVLFLDEFTEFRRDLLESLRQPLEDGFISVSRASGSVTYPARVL
                     FVAACNPCPCGFLGDPVEPCRCSPSAVDRYRRKMSGPILDRIDLCVTVPRLTPDELVQ
                     AEGQGGESSTRVRERVLRAREVQWDRWKNLGYQCNAELPEKALRKQVRLDLDGRRFLK
                     DVAGRIRLSGRGISRVL"
BASE COUNT         1145 a         1418 c         1760 g         1009 t
ORIGIN      
        1 catgacgggg ggacagtcac accccggtat cggcgacaaa ctgaggaagg gcgagaccgg
       61 cagaagggtc gacctggaga gcgcagtgaa gggatgtggc gtcaactggg tcaagaccac
      121 cgaagcctac gacatctcca cctcccggac ccttgtcaag gaggcctggg attacgccag
      181 gacccacgag gaaccggcgg tggttatctt cagacacccc tgcatgctcc tcaggcagaa
      241 gcaggccgtg atacccgtca cggtcgatcc cgagaagtgc ataggatgca agttctgcat
      301 caactttttc aactgccccg gacttgtctt cgacgaaaag tccaaaaagg catacattga
      361 cgaaaggttc tgcgtgaagt gcggagtctg cattaatgtc tgcccccacg gggccatcct
      421 cgcgacagaa ggggaggaag cctagccatg cagtacgtga tagttgggat cggaggacag
      481 gggatactct tttccagccg tgtccttggt catattgccc tctccagggg tgaaagggtc
      541 gtcggaagcg aggtccacgg catggcccag cgtggagggt cggtcataag ccacttcaag
      601 gtaggggatt acagcagccc catggtgaag gcgaaggagg ctgatgtgct ccttgctttc
      661 gaccagaacg aggccgtcag gaacctgcat ttcctgaagc agggcgggca cgccataata
      721 aacactcacg aacccgcctt catcgagaac ccttcgctca ggaagtggat gtccgatcgg
      781 ggcgtcaagg ttcacacggt gatggggtat gacatcctga aaagggaaat gggaggccgt
      841 ttcctcttcc tcaacgtcct tatcctcggg gccatgtgcg gcgcaggagt ggggaacgtg
      901 accattgaag aggtgcgcga ggcagttagg gtgctggcgc ccgcgaaatt cgtcgaagat
      961 aacgtaaaag cggtggatct gggtcatgcg ggagccagta gatgacagaa taacaggatc
     1021 gaagcgggga gccgggaatg ggctccccgc ttaccgggcc ccgagggcaa gaaaagccta
     1081 caccctggtc gagacagccg tagtactctc catcgtaata gcgatctcct ttaccgtctc
     1141 cccgatagta acgggactga tctcacagag ggctgggagt gaacttgccg ccagggccga
     1201 agcaaggaat atggcaagat ggatcgagag gacccttcag aaggcctgcc tgcaccgcag
     1261 ggcttttaac gtcaaatatc tttcaggttt ccggcaggag tttctgatcc tgtggttcga
     1321 cccgcccgaa tatgagagat atcagactaa cgggaggtgt cttgtttact tcaagtcgac
     1381 ctccactcag gataggtgct atactccagc gtggcataca atgacaccgg ccttcacgct
     1441 gtatgtctac actcctggga gatcgaagaa gaagatcgcc gaaatagccg tgtcgggcta
     1501 ctgctcggta agacttacgg agttcccctg aagaccggcg aggatctgat agtttggagg
     1561 gcttgatggt ggcggttaaa ccctggtgga gagagacaat agagactgtt ctgtgggctc
     1621 tggtccttgc gctggtgctg agggctctgg tcatacaggc tttctggatc cctagcggct
     1681 cgatgatacc gacccttctt ccccgtgaca gggtgttggt ggccaagttc tggtattcct
     1741 tcgccgagcc caagcggggt cagataatcg tcttcaagta tcccctggat ccgaagaggg
     1801 atttcgtcaa gaggctcatc ggtctccccg gggagacggt ggagatcgtt gaaggaacgg
     1861 tgatgataga cggcgaaccc ctccaggagc cctatgtgaa gaaccgtgac agcctaagct
     1921 tcggccccct tgaggtcccc gaggggaact acttcatgct gggggacaac agacccaact
     1981 cccaggacag ccgtttctgg ggtttcgtgc ccgaggccaa catcaagggc cctgccttta
     2041 ttcgctactg gcccatacca agggttggag ggctctacaa ggaatgacca ggaccgtatg
     2101 gtacccgggt cacatggccc ggggcctgag gaaactcagg gagctctcgg agaaactgga
     2161 tcttctggta gaggttcgcg atgccagggc tcccgccctg acagggtccc ccttcagcga
     2221 acaggtggcc aggttcaggc cggtctggat agttctttcg aaaaaggacc tggccgtcga
     2281 agaagccacc gccaggtggc tttccttttt tcgagaccag gggggaaagg cctgggccct
     2341 ggatctgagg aaggggaacc tggagcccct gctcaggacg gcggcccggc tcaggccctc
     2401 tcacagggag cttaggctgg ccgtaatagg cattcccaac gttggcaagt cccagctcct
     2461 aaacggcatt ataggcaaga acacggcacc ggtcgggggt atcccgggga tcaccagggg
     2521 agtctcatgg tacaagggga agggattcct ggtggtcgac tctccaggaa tagtggaccc
     2581 gagatcccac agggaggtac accgtaggct ggcctggctc ggcagcagca ggagcgatgt
     2641 gataggaggc tacgaggacc ttgccgaggg actggtccgt ttcctgggtg ggtcgtccat
     2701 gcttgagagg accctatcct cgtgggagat ggaaagactg gacgaaaccc cctccgacac
     2761 tttgcggagg ataggggtaa ggctgggatg ccttgtaaag gggggcgagg tgaatatgga
     2821 tcttgccggg aagaggttca tcgaggcctt ctccacggga aggatcggaa ggttcaccct
     2881 ggaagagccc ggcgacaggc gcccctggga ggacgaaccg tgatagtggc gggggttgat
     2941 gaagctggga gaggacccct tgcaggccct gtcgtggcgg ctgccgtgat ccttgacagg
     3001 ggcgaaacgg cccgcctcct ctcaatgggc ctgaaggatt caaagagact ttcttcctcc
     3061 tcaagagagg ctattttcaa agagatgaag agaatgggcg tcgcctggcg agcccaggcg
     3121 gcctcacctg gaaggataga cagggacaac atactgcagg ctacgctgtg ggcgatgaac
     3181 agaagcgtaa caatgcttcc gagagagccg ggcctggttg tggtcgacgg tagcactccc
     3241 attccggggc ttggtgtccc gcagagggcg ctccccggcg ccgacggcat ggtgccggca
     3301 gtggcagcag cttcggtggt ggcaaaggta ctgagggaca gggccatgtg ttccctggac
     3361 agcattttcc catgctacgg gttccgccgc cacaagggct acgcctcccg ggagcacgtt
     3421 gaggccctcc gccgggaggg tccaagcagg atacacagga ccagcttcca ggtcaggggc
     3481 tggacctagc agattaaagg gttccattca ttcctccgtt cacctggaac gggaatatcc
     3541 ggaaaccgaa agggagaagg aaatggcaaa acatatcgat cttggagccg ccggggaggc
     3601 catggcggcc agggagctgt tgaagcttgg ctacaggctg atcgacacca acgtccgtat
     3661 gaagatgggt gaactggaca ttgtggccga agagggcggg gagatggtcg tggtggaggt
     3721 ccggacaaga accctgggca ggatggtccc ccctgaagct accgtcggcc cttcgaagat
     3781 acggaagctg gtcaggaccg ggagaaccta catggaatcc gtcggatggg aaggcccgtg
     3841 gaggatagac atagcagcca taaccgtggg gtccgatggt agtccgaagc tggaggtctt
     3901 ccgggatatc actgtgggga taattgagcc atgaacgtcg ttcacggcgt caccctacag
     3961 ggtataaagg ccctgaaggt cgaagttgaa gtggagataa cggggggact ctttgtgatc
     4021 gcggtggtgg ggcttcctga tgcctccgtg aaggaggctc gcgagagggt gagggctgcg
     4081 ctgagatctt tgggggtctc agtaaagggg agggtggccg tcaacctggc gccggccgac
     4141 agccccaagg agggtgcact cctggacctc cccatagccg taggaattgc tcacgccaag
     4201 ggggagatca gggtgcccgg gcccactctt ttcatgggtg agctcgcact ggacggcagg
     4261 gtcagaaggg tcaggggagc ggttccggcg gcacttctgg caagagatct ggggttcccg
     4321 ctcttcgtcc ccgagggcaa cgcccgggag gtctccctgg taaagggggt cagggcctgg
     4381 caggtcgggc atatccgtga actcttcgct cacctcaggg ggcaaagcca gctcaaggtc
     4441 attgagcagg ctgaggaggc taccggcgac cccgagccgg accccgactt ttcagacatc
     4501 aaggggcagg ttgctgccaa gagggctctg gagatagccg cggcaggcca ccataatgtt
     4561 cttctcactg gctctcctgg ctcaggcaag acccttcttg caagggccct taaggacata
     4621 ctccccccgc tctccgatga agagatgatg gaagtgctcc tggtgagaag taccgtcggg
     4681 cttccccttg acagctcccg tcgccgtccc ttcagggcaa ttcaccacac ctccagtacc
     4741 atttcggtct gcggaggtgg gacgaacctg cgacccgggg aggttagcct ggctcacagg
     4801 ggagtcctgt tcctcgacga gttcaccgag ttcaggagag acctgctcga gtccctgagg
     4861 cagcccctgg aggacggctt catctccgtg agcagggcca gcgggagtgt gacctatccc
     4921 gccagggtgc tgtttgtggc cgcatgcaat ccatgcccat gcggcttcct gggggatccc
     4981 gtcgagccgt gccggtgttc tccttctgcc gtggaccgtt accgtcgcaa gatgtcagga
     5041 cccatacttg acaggatcga tctctgcgta accgttccga ggcttacgcc cgacgagctt
     5101 gtacaggccg aggggcaggg gggagagagc agcacccggg tgagggagag ggtcctgcga
     5161 gcgagggagg tccagtggga tcggtggaag aacctgggat accagtgcaa cgccgagttg
     5221 cctgaaaagg ctctcaggaa acaggtaagg cttgacctcg acgggaggcg ttttctgaag
     5281 gatgtggcgg gaaggatccg cctctccgga agggggatca gcagggtcct ca
//
LOCUS       DAOPKQ010000085         5323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_16729_length_5323_cov_4.169894, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000085 DAOPKQ010000000
VERSION     DAOPKQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16729_length_5323_cov_4.169894"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1216)
                     /locus_tag="PK146_04100"
     CDS             complement(194..1216)
                     /locus_tag="PK146_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE91456.1"
                     /translation="MTNIYELMDVTQSYGGRTVLEVPEMKIGKGSIVGITGPNGSGKS
                     TLLRILAFLERPVGGRVYFKGEPVIDIDETLRREATLLLQESKLLKRTVFENVAYGLK
                     ARGMKEGISRKVNQTLEMVGLDPVDFAGRLWFQLSGGESQRVALASRLVLRPEVLILD
                     EPTASIDAGSGRIIARAIVDARERLGTTIVLVSHDIDWAYAASDEVISMFSGRIRSRT
                     IENIIPGPWKDCPDGTSCRYLENGQVITAVRRPEEGYSTGLLDPDDIILSLNDPGNTS
                     ARNTIRGTVVRMNLENEEGNVLVTVNAGGTVFTSRVTTGSLSKLGIVPGSPVIIVFKA
                     NSIRWV"
     gene            complement(1213..1908)
                     /locus_tag="PK146_04105"
     CDS             complement(1213..1908)
                     /locus_tag="PK146_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91457.1"
                     /translation="MGYLAEGFRKAFIILFSGQEETFSAIFTTLKVSTFSIIVTLAVG
                     IPLGFMLGYYTFPGKHAFRAVSDTLMAIPTVVVGLLVYAFISRSGPFGSFGLLFTLRG
                     VAIGQFVLGLPVVVSLTASATEQMEDSVKMTLMTLGASPGQLAASSLWEGRYAVIAAA
                     MTAYSRIISEVGVSMMIGGNIKWRTRTITTAIALETGKGQFAEGIALGIVLLAIAFAV
                     NGFVSVARRRASI"
     gene            complement(2065..2889)
                     /locus_tag="PK146_04110"
     CDS             complement(2065..2889)
                     /locus_tag="PK146_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPE91458.1"
                     /translation="MKRSVRVLLLSLVVLSIAVTAAFAAPILRMATTTSTDNTGLLDA
                     LAPEFLKDTGIELQWVSVGTGKALELGKNLDVDVLLVHAPDLEKQFVEDGYGVNRREV
                     MYNDFVIIGPASDPAKTMGKTVKDAFIAIAGKKANFASRGDNSGTHAMEIDLWKRSGI
                     EQPEKESWYIQTGQGMLNTINIAAEREGYTLTDRGTFIKYEDNHKGNPPLVILVEGDD
                     VLFNQYSVIAVNPVRGDHLRYDLALKFSDWMRSDKVQKMIGNFTLLGKPLFVPNAK"
     regulatory      complement(2979..3097)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3302..4492
                     /locus_tag="PK146_04115"
     CDS             3302..4492
                     /locus_tag="PK146_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE91459.1"
                     /translation="MKDRQAGMDAIHSGDRVLEGVSRPKVPPIFASSVFSFDSLSDLD
                     SVFDQKKPGYIYARMGHPNASLLEETISALEGTDAALVFSSGMAAIATAFLAALKPGD
                     QVIADRVLYGGSFSFIDGFLRDWGVSVELVDTLDHDQVREALCPKTKVIYLETISNPL
                     MGVADLPALAEIAASAGAMLFVDNTFASPALCRPASMGADVVIHSLTKYINGHSDVTG
                     GALAGSEEFVAGASRLRSMMGGSMSPFDAWLVQRGVRTLHLRMAAHSANALQIARTFE
                     RHPRVERVEYPGLERHPSYGTARKVLEGGFGGMLSFEIRGGERAAARFVDGLRLVELV
                     PSLASISTTISHPAKTSHRSLSEDQRRESGISDGLIRLSAGIEPVEAVLEDLLQALDW
                     IGGE"
     gene            4516..>5323
                     /locus_tag="PK146_04120"
     CDS             4516..>5323
                     /locus_tag="PK146_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPE91460.1"
                     /translation="MKKVLVTFPEDRAGDLALADALGGVAEISRIPERGDPSRSGALK
                     EADAVISFMMHNELDPGEFSLLKEGCLLQSMQAGVDALPFSDIPERVTICANTGAWAD
                     PMAEHILGMILSLGKYLQKCHDRLGKGQFDRSIVSTWFRGKTCGFVGYGGIAKAVARL
                     VRPMGMRVIAVNTRGETDEDIDLIGTMDDLPRILQESDVLVIAVPLTRKTLGLLGEKE
                     LAMMKEDAILVNPARGAIIDQKALYERMKAFPEFRVGIDAWWVEPHTHGEF"
BASE COUNT         1182 a         1400 c         1540 g         1201 t
ORIGIN      
        1 gtatcggtgc tctggggtac gttgccagga aagagccgag aaaggatata ctgacggtca
       61 tccagtcctg cataggaatg ggcagttata tggtcttcgt tctgaacagg tcagccctgt
      121 cgacctacta cccatggctg gtggattact tctccaggta gacctctggg aggggaggtg
      181 atttcttctc ctctcagacc caacgaatgg agtttgcctt gaaaacaatg atcacgggcg
      241 atcctgggac gatgcccagt tttgaaaggg aacctgttgt aaccctggat gtaaagaccg
      301 ttccccccgc gttgaccgtc acaaggacgt tcccttcctc gttctcaagg ttcattctta
      361 caactgttcc ccggatggtg ttccttgccg aggtgttgcc ggggtcgttc aaggaaagga
      421 tgatgtcatc agggtcaagc aatcccgttg aatagccctc ttccggtctc cttaccgccg
      481 ttatgacctg cccgttctcg aggtatctgc aggaggtccc gtcagggcaa tccttccagg
      541 gaccggggat tatgttctcg atggtcctgg aacgtatcct tccggagaac atggagataa
      601 cttcatcaga ggccgcatag gcccaatcga tatcatgact gacgagcact atggtagtgc
      661 cgagcctttc cctggcgtcg acgatggccc gggcgattat cctcccgctg cctgcatcga
      721 tgctggccgt cggttcatcg aggatgagta cttcaggcct caggaccagc ctggaagcca
      781 gggcaactct ctgggactca cccccggaga gctggaacca gagccttccc gcgaaatcaa
      841 cgggatcaag acctaccatc tccagggttt ggttcacctt cctggaaatc ccttccttca
      901 ttcccctggc cttgaggcca taggcgacgt tttcgaagac ggtcctcttc aacagtttag
      961 attcctggag cagaagagtg gcttctctcc ttagagtctc gtcaatatca ataacgggct
     1021 ctcccttgaa atatacccga ccgccaacag gcctctcaag aaaggccagg atcctcagaa
     1081 gggtactctt gccgctaccg ttgggacctg ttatgccgac gatagagcct ttcccgatct
     1141 tcatctccgg cacttcgaga acggttcgcc ccccgtagga ttgggttacg tccatgagct
     1201 cgtagatatt cgtcaaatgg aagccctcct cctggcgacg gaaacgaacc cgttcaccgc
     1261 aaaggcgata gccagaagta cgatgcccag ggcgatgccc tccgcgaact gtccctttcc
     1321 tgtctcaagg gcgatggcgg tggttatggt ccgggttctc cacttgatgt tgccacctat
     1381 catcattgag acgccgacct cggagataat cctgctgtag gccgtcatgg ccgcggcgat
     1441 cacggcgtag cgtccctccc atagagagct ggcggcaagt tgacctgggg aagcaccgag
     1501 cgtcatcagc gtcattttca cggaatcctc catctgttcc gtagccgaag ctgtcaggga
     1561 aacaacgaca gggaggccca ggacgaactg acctatggca acacctcgaa gggtgaagag
     1621 aagaccgaag ctcccgaagg ggccgctcct ggaaatgaat gcatagacca gtaatcccac
     1681 caccaccgtg ggaattgcca tgagggtgtc cgatacggcc ctgaaagcgt gcttgccagg
     1741 gaaggtgtag taacccagca tgaatcccag cggtatacca acggcaagag tgacaatgat
     1801 cgagaaggtc gagaccttga gagtcgtaaa tatggcgctg aaggtctctt cctgtcctga
     1861 aaagaggatg atgaatgcct ttctgaaacc ttctgccaag taacccatgg ctacctccgg
     1921 agtgatctgt cctggttttt gcctttagat gttctgacaa caatgatctt aaaccattct
     1981 tcaggcccct tcaaaaaggg accccgcaag atccccaggg gggtggggaa gatgcggggt
     2041 cccctgccag ttggcaaggt aagtctattt tgcgttggga acgaagagag gtttacccag
     2101 cagggtgaaa tttcctatca tcttctggac cttgtcagag cgcatccagt cggagaactt
     2161 cagggcgagg tcgtagcgaa ggtggtctcc cctgacgggg ttgacggcga tgacgctgta
     2221 ctggttgaag aggacatcgt caccctctac aagaatgacc agcggagggt tacccttgtg
     2281 gttatcctcg tacttgatga aggtgcccct gtcggtcagg gtgtaacctt ccctctctgc
     2341 tgcgatgttt atggtattga gcatgccctg gccggtctgt atataccagc tctccttttc
     2401 gggctgttcg atcccggacc tcttccagag gtctatctcc atggcgtgag ttccagagtt
     2461 gtcaccgcgg ctggcgaagt tcgctttctt gccggcgatg gcgataaatg cgtctttgac
     2521 ggtctttccc atggtctttg cgggatccga agccgggcca atgataacga agtcgttgta
     2581 cattacttcg cggcggttga ccccgtaacc gtcctcgaca aattgctttt caaggtccgg
     2641 agcatgcacc aggagtacgt cgacatcgag gttcttaccc agctcaaggg ccttgccagt
     2701 ccccaccgag acccactgga gctcgatccc cgtatccttg aggaactccg gggccagagc
     2761 atcgagaagc cctgtgttgt ctgtactggt agtggtggcc atgcggagga tcggagctgc
     2821 gaaggccgct gtaaccgcaa tggacaatac cacgagggag aggagaagaa cccttactga
     2881 acgcttcatg caaaccaacc tcctgaaggt ggaattacgg agagatctcc ggtaacaaaa
     2941 aaccgtctcc ctcaggaaag ggggagacgg tccagaagtt cggtccttct cttcctcctt
     3001 tccaaacacg ggaggcgccc ctgtttccgg gaacgccctt cggcctgtcg ccatagggaa
     3061 gccctttagg cggtcaggct cggaggtttt tttgttacag ggaatatacc acgtatcgaa
     3121 gggcaggtaa agagttcgtt actgttcgcg tggaatgacg atagtaccaa atcggaaggc
     3181 cggctgaaac ggtcggaagg tgagtatgat ccaaaaaatt acctataggg gtatatctat
     3241 ttcaaaatag gggctataat attcggactg gctaatataa atgaacgaga gggggtagca
     3301 catgaaagac aggcaggcag gtatggatgc gatccactcg ggggacaggg tcctcgaggg
     3361 agtttcgagg ccgaaggtcc ctcctatttt cgccagttct gttttctcct ttgattcgct
     3421 atccgacctg gatagtgttt ttgaccaaaa aaagcccgga tacatatatg cccgtatggg
     3481 tcatccgaac gcctcccttc tcgaggagac catctcggcc ctggagggga ccgatgccgc
     3541 tcttgtattt tcctcgggga tggcggcaat tgcaacggct ttcctggcgg ccttgaagcc
     3601 gggggaccag gttatagccg acagggtcct ttacggaggc agcttttctt ttattgacgg
     3661 atttctcagg gattgggggg tgtcggtgga gctggtggat accctggatc acgatcaggt
     3721 ccgtgaggct ttatgtccaa agacaaaagt aatctatctg gagactatct ccaacccact
     3781 tatgggtgtc gcagacctcc cggccctggc ggagatagcc gcttctgccg gagcgatgct
     3841 cttcgtagat aacaccttcg caagtcctgc cctctgcagg cctgcctcaa tgggtgccga
     3901 tgtggtcatc cacagcctta cgaagtatat taacggacac agcgatgtga caggcggggc
     3961 tctggcaggg agtgaagagt tcgttgccgg ggccagtcgt ttgcggtcga tgatgggcgg
     4021 aagcatgagc ccctttgatg catggctggt acagaggggt gtcaggaccc tgcacctgag
     4081 gatggcagcc cactcggcta acgcccttca gatagccagg accttcgaga ggcatcctcg
     4141 ggtagagagg gtcgaatacc ccggtctgga gagacatcca agctacggaa cggcccggaa
     4201 ggtgcttgaa ggaggtttcg gcggaatgct cagtttcgag ataagagggg gggaaagagc
     4261 cgcagcacga ttcgtggatg gtctgagact tgtggaactg gtgcccagcc ttgcttccat
     4321 atcgacgacc atttcccacc cggcaaagac atctcaccgt tctctttcag aggaccagcg
     4381 gagggagtca ggcatatcgg acggtctgat caggctctcc gcaggaatcg agcccgtgga
     4441 ggccgttctg gaggatctcc tccaggctct tgactggatc ggtggagaat aagagaaaaa
     4501 ggaggaggct actcaatgaa gaaggtgctt gttacttttc ctgaagacag ggcaggtgat
     4561 ctggccctgg ccgatgccct gggaggcgtg gcagagatct ccaggatccc cgagagaggg
     4621 gatccctcgc ggtctggcgc cctgaaggag gcagacgctg ttatttcctt catgatgcac
     4681 aacgagctgg atcccgggga gttctctctc ctgaaagaag gctgtctcct acagtccatg
     4741 caggcaggag tggatgctct tcccttcagc gatatccccg aaagggtgac catatgcgcc
     4801 aataccggag cctgggcaga tccgatggcg gagcatatcc ttggaatgat cctgtcgctg
     4861 gggaaatacc tccagaagtg ccatgatcgt cttggcaagg gacaatttga ccgcagtatc
     4921 gtcagcacgt ggttccgtgg aaagacctgc gggttcgttg gctacggagg tatcgccaag
     4981 gccgtggcca ggcttgtccg ccccatgggg atgagggtca ttgcagtgaa caccagggga
     5041 gagacagacg aggatatcga tctcatcgga accatggatg atctgccgag gatccttcag
     5101 gaatcggacg ttctggtgat cgcagtaccc ttgaccagga agaccctggg ccttttgggc
     5161 gagaaagaac ttgccatgat gaaagaagat gccatcctgg tcaaccctgc ccggggtgcg
     5221 ataattgacc agaaggcgct atacgagaga atgaaagcct tccccgaatt tcgtgtaggg
     5281 atagatgcct ggtgggttga gccccatacc catggggaat tca
//
LOCUS       DAOPKQ010000086         5305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_16800_length_5305_cov_6.115429, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000086 DAOPKQ010000000
VERSION     DAOPKQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16800_length_5305_cov_6.115429"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PK146_04125"
     CDS             complement(<1..838)
                     /locus_tag="PK146_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPE91461.1"
                     /translation="MLNCGITGLPLSGKSTIFNVITRAGAEVKPYAGGKTDPNRAVVS
                     VPDDRFNRLVEIYQPKKITPAPVEFVDLAGLSRDASKGAGLGNSFLSFVAESEALIQV
                     IRCFDNPEVPHPEETVNPVRDWNIVETELILRDLSVIENRLGKLQEKKKPSPEEQAEK
                     ALLERCRDHLFEERPLREMGFSPDEARTVRGFTFLTLKPELVVLNMDESQASGTMPWM
                     AEMEKLAADRGLSLVRLFGRMEMEIADLDVSEQAEFMSELGISEPGRERLIREAYSLL
                     GLI"
     gene            958..2478
                     /locus_tag="PK146_04130"
     CDS             958..2478
                     /locus_tag="PK146_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPE91462.1"
                     /translation="MNKGRSFLAVFTVAAVLAAGGAASGAENSLAAGKVDVYPRGALL
                     SIEMEAEPLMQIELPLTLDPGNMTVTAGEGVTVQGYDIVEAARPGWVPPALASLADEL
                     DKARSRVDMLRARASALEQARKHLGEAVPENLKGSELEAFVDTAVKKRESVELKAAET
                     EILLEKAMKEHDLLRSEYESQFPGDPDRVLLLSLETEGKGKINLEAWTREAYWRPSYR
                     MGLNSSTGEVTVKLAADVSQKSGITWNGEILLHTVRPKGGINIPEMRPLVVDFLEISP
                     APKAMRGAMEEMAASVMMDEAVPQEPLMEEGLTDVTLKARGIVNGFGKKEMLELGKFS
                     EKSEVSLVAIPEFSSEAWTVSTVKSLGRSILPGEARLSVDGKETGVTNIPRRSMGQEL
                     QIPFGTTPLVTSRREEMLPEKGSSWIIRGKHQRGYVITVSNGLREKVTLKVMDRIPVP
                     ARDNIKLQDLVVSPEPAEKIEKGLVTWNVELEKGQSSEISVKYTVTYPADKELMFR"
     gene            2500..3027
                     /locus_tag="PK146_04135"
     CDS             2500..3027
                     /locus_tag="PK146_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPE91463.1"
                     /translation="MNEERISEGIEEYLGRLKSRGFRLTNQRRVIVETLLRHLGTHLN
                     ARELLDLAQVEDPSIGIATIYRTIELLNRLGMLNMVNLEEGFLRFEVPDDRMHFHIFC
                     RSCGRTVHLSDEDGKMEEVLRWAKEEGFDLLPQTFEMAGLCRDCREGGILETEEFPPG
                     MGRRRCRRRFGGLKA"
     gene            3097..3480
                     /locus_tag="PK146_04140"
     CDS             3097..3480
                     /locus_tag="PK146_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91464.1"
                     /translation="MTARRNILTAVLVVAIAFSGTAFASVGEELVGNYRGTFIGDDYG
                     SFLIDVGPDGSITGTVHSNVSFLDMDVKGTCAPNGECEFVTEGEEAVPFYFMGRIDFM
                     NRFIGKWSYSDHSGRGSFYGMIQKE"
     gene            3512..>5305
                     /gene="glgB"
                     /locus_tag="PK146_04145"
     CDS             3512..>5305
                     /gene="glgB"
                     /locus_tag="PK146_04145"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPE91465.1"
                     /translation="MRYDTSLLTEQDIYLFREGRHCRLYERLGAHFTDLPEGEGTHFA
                     VWAPGAGEVSVIGDFNGWDRGSSPLKPRWDSSGIWEGFVPGAVHGARYKYFIKSSDGR
                     WSGEKADPLGRFMEPPPGTASVIWDDTHDWKDGQWMENRSGSSALNRPISVYEIHPGS
                     WRRGEDGEFLSWRQMAPLLVDYISGTGFTHVELLPVMEHPFYGSWGYQTTGYFAPTAR
                     YGTPSDLKYLVDVLHQAGIGVILDWVPSHFPDDPHGLARFDGSCLYEHSDPRQGFHPD
                     WKSCIFNYGRNEVRSFLLSSAAFWADRYHADGIRVDAVASMLYLDYSRKEGEWVPNIH
                     GGRENLEAMQFLRDLNVSLYRDFPGIQTIAEESTAWPMVTRPVHTGGLGFGLKWNMGW
                     MHDTLRYFSLDPVFRKFEHNLLTFSAWYAFSENFMLPLSHDEVVHGKGSLFARMPGDD
                     WQKFAGLRLLLGYMYAHPGKKLIFMGGEFAASREWDHESSLDWHLAGMGPNAGVTLWI
                     SDLNRIYRREPALHEKDCFHEGFSWLDARDRDSSIVSFLRFGGEEAVVAVCNFTPVPR
                     HGYVLGVPSGGRWEEMLNSDAECYGGSGQSNM"
BASE COUNT         1222 a         1344 c         1598 g         1141 t
ORIGIN      
        1 aaatgaggcc cagaagagag taggcctccc ttataagacg ttctctccct ggttcgctga
       61 ttcccagttc agacatgaat tctgcctgtt ccgagacgtc aaggtctgct atctccatct
      121 ccattcgtcc aaaaagccgc accaggctga gcccccggtc ggctgccagt ttttccatct
      181 ctgccatcca gggcatggtt ccagaggcct gggactcgtc catgttgagg acgaccagct
      241 ccggtttcag ggtcaggaat gtgaaccccc ggacagttcg ggcctcgtcg ggggaaaagc
      301 ccatctctcg aaggggtctc tcttcgaaaa ggtgatcccg gcacctttcg aggagggctt
      361 tctcggcctg ctcttcgggg gacggtttct tcttttcctg aagtttgcca agcctgttct
      421 cgataactga caggtcgcgc aggatcagtt cggtctcgac gatgttccag tcgcggactg
      481 ggtttacagt ttcctcggga tggggcactt cggggttgtc gaaacagcgg atgacctgaa
      541 taagggcctc cgattccgcc acgaaggaga ggaacgagtt gccgagaccg gctcccttgc
      601 tggcgtcgcg ggataatccc gccagatcca cgaattcgac cggtgccgga gttatctttt
      661 taggctggta gatctcgacc agtctattga acctgtcatc cggaacggaa acgacagccc
      721 tgttcgggtc ggtctttccg ccagcgtagg gcttgacctc cgctccggcc cgtgttatga
      781 cgttgaagat ggtactcttt ccactaaggg gcaatcctgt aatgccacag ttcaacattc
      841 ctttcacctc ttatatgcca gtttatcatc aggggcctgt aagtgtatga taatagagtc
      901 tggaacttca cgcgcggttt gtaaacctca aaaagatcca gatcgggagg gagaatcatg
      961 aacaaaggga gatcgtttct ggcagtcttt accgttgcgg ctgttctcgc cgcggggggc
     1021 gctgcttccg gtgccgaaaa cagtttggcg gcggggaagg tggatgtcta tcccaggggt
     1081 gctctactct ccatcgaaat ggaagccgaa ccccttatgc agatagaact ccccctgacc
     1141 ctggatccag gaaacatgac agtaaccgct ggtgagggag tcacggtcca ggggtacgat
     1201 atcgtagagg cggcaaggcc ggggtgggtc ccgcctgccc ttgcctctct ggccgatgaa
     1261 cttgacaagg ctaggtcaag ggtcgacatg ctaagggcca gggcctcggc cctggagcag
     1321 gcaaggaaac atcttggcga ggccgttccc gagaatctca agggatcgga actggaagct
     1381 ttcgtcgaca cggcggtcaa aaaaagggaa tccgttgaac tcaaggcggc cgagaccgag
     1441 atccttctgg agaaggccat gaaagagcat gacctgctcc gctccgaata cgaaagccag
     1501 tttccgggag atcccgacag ggtgcttctt ctctcgctgg agacggaggg caagggcaag
     1561 ataaatcttg aagcctggac gagggaggcc tactggagac cttcctacag aatgggactc
     1621 aacagctcga cgggcgaggt gacggtgaag cttgcggccg acgtgagcca gaagagcggt
     1681 ataacctgga atggggagat ccttcttcac accgtaagac ccaagggtgg tatcaacata
     1741 cccgagatgc gccccctggt cgtcgatttt ctggaaatct ccccggcacc taaagccatg
     1801 cggggagcta tggaggagat ggcggcatcg gtgatgatgg atgaggcagt tccacaggaa
     1861 cccctgatgg aggagggcct gacagacgta acattgaagg ccaggggaat cgtaaacggt
     1921 ttcggcaaga aggagatgct ggaattgggc aaattctccg aaaaaagcga ggtctccctt
     1981 gttgccatac ccgaattttc cagcgaggca tggacggttt ccaccgtaaa atcccttgga
     2041 aggtccattc ttcccggtga ggcaaggctt tcggttgacg gtaaggaaac aggggttacc
     2101 aacataccaa ggcggagcat gggtcaggaa ctacagatac ctttcgggac cacacctctg
     2161 gtgacctcca gaagggaaga gatgcttccc gagaagggca gcagctggat cataagggga
     2221 aaacaccagc gaggatatgt cattacggta agcaatggcc tcagggagaa ggtgaccctc
     2281 aaggttatgg accgaattcc cgttcccgcc agggacaaca ttaaactaca ggatcttgtg
     2341 gtttcgcctg agccggccga gaagatagaa aaaggtctgg tgacctggaa tgtggaactg
     2401 gaaaaaggtc agagcagcga gataagcgtc aaatacactg tgacctatcc cgccgacaag
     2461 gaactgatgt tcagatgatc cggcaacggg agagatgtca tgaatgagga acggataagc
     2521 gaaggaatag aagagtatct tggcaggctc aagtcccggg gcttcaggct tacgaaccag
     2581 agacgcgtca ttgtggagac ccttctgcgg cacctcggga cacacctgaa cgcgcgggaa
     2641 ctgcttgatc tcgcccaggt cgaggatcct tcgataggta ttgccacaat atacaggact
     2701 atagagcttc tcaacaggct ggggatgctg aacatggtca accttgaaga agggtttctg
     2761 cgcttcgagg ttcccgatga caggatgcat tttcatatct tctgcagatc ctgcgggagg
     2821 acggttcacc tttccgacga ggatggaaag atggaggagg tcctccgctg ggcaaaagaa
     2881 gaaggattcg atcttctgcc ccagaccttc gaaatggccg ggctatgcag ggattgccgc
     2941 gaggggggca tcctcgagac agaagagttt ccgcctggta tgggaaggcg taggtgcaga
     3001 aggcgtttcg gtggtttaaa agcatagtca ggctcaagga tgacagaatc attctgtctc
     3061 cgggtagatg tcaaaaaaga aagggagtgg aggagaatga cagcaagaag aaatatcctg
     3121 accgccgttc tcgtggtggc gatagcattt tccggtacgg ctttcgcttc ggttggagaa
     3181 gaacttgtag ggaactacag gggtaccttc atcggcgatg attacggatc gttcctgatc
     3241 gatgtcgggc ccgacgggag catcaccggt actgtccatt ccaacgtctc cttcctggat
     3301 atggacgtaa agggaacctg tgctccaaac ggagaatgcg agttcgttac agagggtgaa
     3361 gaagccgttc ccttttactt tatgggaagg atagacttca tgaaccgttt catcggcaag
     3421 tggtcctatt ccgaccactc cggccggggt tccttttacg ggatgatcca gaaggagtga
     3481 accatggcct gaaagaaaac gggaaagcat aatgcgctat gacacttcgc ttctgacgga
     3541 gcaggacata tatcttttca gggaagggcg ccactgccgg ctctacgaga ggcttggcgc
     3601 ccattttact gatctcccgg agggggaagg tacccatttt gccgtctggg ctcccggcgc
     3661 gggagaagta tccgtcatcg gagatttcaa cggctgggac agaggttcat ctccgctgaa
     3721 accaaggtgg gattcctcgg gaatatggga aggtttcgtt cccggggcgg tccatggagc
     3781 gaggtacaag tatttcatca agtcttcaga cggcagatgg agcggtgaaa aggcagatcc
     3841 cttaggccga ttcatggaac ctccgccggg gacggcctcg gtcatctggg atgataccca
     3901 tgactggaag gacgggcaat ggatggaaaa ccgttcggga tccagtgccc tgaacagacc
     3961 catctctgtc tacgagatcc atccaggttc atggagacgt ggagaagacg gtgagtttct
     4021 ctcctggcgg cagatggcac ccttgctggt cgactacatt tccgggaccg gcttcaccca
     4081 cgtagaactc cttcctgtca tggagcaccc cttctacgga tcctgggggt accagacaac
     4141 ggggtacttt gcgccgacag cgagatatgg cacgccctcc gatcttaagt accttgtcga
     4201 cgtgctccac caggcgggga tcggggtgat cctggactgg gtcccatctc atttccccga
     4261 tgaccctcac gggctggcgc gtttcgacgg gtcctgtctt tacgaacata gtgacccgcg
     4321 gcagggtttt cacccggact ggaaaagctg catcttcaac tacggccgca atgaggtaag
     4381 gtcatttctt ctcagcagcg cggccttctg ggccgacagg tatcatgccg atgggatcag
     4441 ggtagatgcc gtggcctcca tgctttatct ggactactcg agaaaagagg gcgaatgggt
     4501 tccgaacatc catggtggca gggagaatct cgaggccatg cagttcctga gagatctcaa
     4561 cgtctcgctg tacagggatt ttccagggat ccagaccata gccgaagaat ccaccgcatg
     4621 gcccatggtg accaggccgg tccacaccgg ggggctgggc ttcggactca aatggaacat
     4681 ggggtggatg catgacaccc tcaggtattt ttcgctggat ccggttttca ggaaattcga
     4741 gcacaacctg cttactttct ctgcctggta cgccttctcg gagaacttca tgcttcccct
     4801 ttcccacgac gaagtggtcc acggaaaggg atcgctgttt gccaggatgc ctggagacga
     4861 ctggcagaaa ttcgccggat tgagacttct cctcgggtac atgtatgccc accccggaaa
     4921 gaagctgata ttcatgggcg gggagtttgc ggccagtcgt gagtgggacc acgaatcatc
     4981 ccttgactgg catcttgccg gcatgggacc gaatgccggg gtaacgctgt ggatcagtga
     5041 cctcaacaga atctacagaa gagagcccgc ccttcacgag aaagactgct ttcacgaggg
     5101 gttcagctgg ctggatgcca gggacaggga ttcgagcatc gtatccttcc tgcgattcgg
     5161 tggcgaggag gctgtagtgg cagtctgtaa tttcactcct gttcccaggc atggctatgt
     5221 gcttggggtt ccgtccgggg ggcggtggga agagatgctg aacagtgatg cggagtgcta
     5281 cggtgggagc ggccagagca acatg
//
LOCUS       DAOPKQ010000087         5304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_16816_length_5304_cov_4.865308, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000087 DAOPKQ010000000
VERSION     DAOPKQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16816_length_5304_cov_4.865308"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..354)
                     /locus_tag="PK146_04150"
     CDS             complement(88..354)
                     /locus_tag="PK146_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPE91466.1"
                     /translation="MGYNLLHIGFGNMIVIERVVAIIHPSSAPIKRLKDEARSQGRLI
                     DATEGRKTRAILITDSNHVVLSAIQPETIGVRFEGKEKEGEPDR"
     gene            complement(359..1246)
                     /locus_tag="PK146_04155"
     CDS             complement(359..1246)
                     /locus_tag="PK146_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPE91467.1"
                     /translation="MLKSMTGFSRTEERTGLGTFALEISSVNHRNQEISVRLPREISH
                     LEPMIQQKLRSSSRRGKIQARVDISWDSAVRASRIESEVLKGYAQEMQDIRDQLGLKG
                     EVDLELLLTLPGAVSSQETDSNELEGVLRDAVGTILDRGIEEWDSMRAKEGKHLQEAI
                     LGSMIQFRNVTGVIEGKWSTARDKALGSLRERVSQVLGANGPAVDESRIAQEIVLLSD
                     KWDITEEIARSSSHVDKFLGLVHNDEVSGKTLDFLLQEMNREINTIASKVQDPEVRWA
                     AVEAKGLLESIREQVQNVE"
     gene            complement(1240..2373)
                     /gene="hflX"
                     /locus_tag="PK146_04160"
     CDS             complement(1240..2373)
                     /gene="hflX"
                     /locus_tag="PK146_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPE91468.1"
                     /translation="MIDLRQLSSRKAFLVALDEDDSLDVPGSLEEMSLLLGNLGITTT
                     GISIQRRSRPDPGSFIGSGKAELVGAEARDLGAGLIVVNGSLSPGQAAALGRLTGLAV
                     WDRPLVIMKIFEDRAQTGEAKLQIELARCRYEIPFIKGLGLQMSRPGGGVGTRGPGET
                     EFERHRRKLERRIREINRKIEAIRERRKDQRKHRIRAGLATVALTGYTNSGKSTLLRR
                     LSGDSDLPVADKLFSTLDPFIRRVRLPGGDPALFADTVGFIRDLPPDLVTAFRATLEE
                     VAAADLVILVADPSSGRYEKDVEVVEEVLASIGAGTVPRIIALNKIDRLDPETVEPIR
                     ALAGRSGDRVVPISALRGDKIPELLSVVEEMLSMSRGKECASC"
     gene            complement(2370..3005)
                     /locus_tag="PK146_04165"
     CDS             complement(2370..3005)
                     /locus_tag="PK146_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPE91469.1"
                     /translation="MRTLFYWTIALFFRLVMKVYNRVKVIGLDGALAASPPVIIAANH
                     CSNLDPIVIGAFFPRRLRYLAKSELFSFAPLGLAIRALGAVPAKREDLQGAAGALKFL
                     VERLQSGEDVLLFPEGQRSHDGRLKPLEEGVGLLAAKTGAPVIPAYIHGTFEAMPRGS
                     STYRPVKVVLSFGRPIYPGSPGTEGFSSKELRRSVTDNLERSLRELEERSF"
     gene            complement(3029..3709)
                     /gene="cmk"
                     /locus_tag="PK146_04170"
     CDS             complement(3029..3709)
                     /gene="cmk"
                     /locus_tag="PK146_04170"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPE91470.1"
                     /translation="MGTTDPCNENIITIDGPAGAGKSTIARLVAKRLGYEYLDTGAIY
                     RGITYFLSGRSIRPSEGGNIAHALEECTISIEKGRVFIDGVDVSDKIRSPEIDLAVSA
                     YSALHTVRSFLLQIQKRAASGRKMVAEGRDMGSEVFPEAPIKFYLDASLEERATRRWR
                     ELNAKGVNITLEEVRKQVQRRDQLDSEREFSPLRVPEDAHIIDTSKRSPEEVVNVILA
                     VIEDQRAC"
     gene            complement(3730..4461)
                     /locus_tag="PK146_04175"
     CDS             complement(3730..4461)
                     /locus_tag="PK146_04175"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPE91471.1"
                     /translation="MTQGVRINRFIAACGVCSRRKADLLVMAGRISVNLEVCTDPSRT
                     IDTKSDVVKLDGEVISIRPKLYIAMNKPRGLVCSVSDSFSRTVLELLPSEIPVEGLFP
                     VGRLDKGSEGLLLLTNDGEMAQRILHPSHQILKTYEVLLDATADDAALESFMKGTEVE
                     GRKVRPERVVAIPRRPEGRWISVTLSEGLKREIRVMAATRGFKVVRLVRTRIGKLDMK
                     RLRPGEFMILDGEVLMKMILYGGSV"
     gene            complement(4458..5045)
                     /gene="scpB"
                     /locus_tag="PK146_04180"
     CDS             complement(4458..5045)
                     /gene="scpB"
                     /locus_tag="PK146_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPE91472.1"
                     /translation="MSGYPVETPLSPIARSVEAVLFVAVQPVGVKELASVAGVTEEAV
                     ERVLEEIKERYRKCHGLCLLNIAGGWSMATSPEQEEVVTLFRDVALSQRIRLSKAALE
                     SLAVIAYSQPVTRSEIEEIRGVRCERVLDTLLKHGLIRVAGRRKGSGTPLLYRTTNSF
                     LSLFGLGSISDLPTLDEMQDILSQDEYPEDGERAT"
     gene            complement(5026..>5304)
                     /locus_tag="PK146_04185"
     CDS             complement(5026..>5304)
                     /locus_tag="PK146_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91473.1"
                     /translation="KREGTRSLATGLEEEWDGIPAATPEEEQVEGRMSEIEMLLSSRG
                     ALLLSEVLGTPCSRHLLVVTLLALLEMARLGRVRLHQEELFGDVRISG"
BASE COUNT         1087 a         1651 c         1307 g         1259 t
ORIGIN      
        1 tccagaccct tgacggcatc aaaaagagct ttcctgaggg tccctttccc caccccgcta
       61 gggccggaga tcacgaaaag cctcccccta tcgatcaggt tcgccctcct tctccttacc
      121 ctcgaacctg acgcctatag tctctggctg gatcgccgac aggaccacgt gattactgtc
      181 ggttatcagg atcgccctgg tcttgcggcc ttcagtagca tcgataagcc tgccctgact
      241 tctcgcttca tccttcagcc gcttgatcgg agctgaggag ggatggatga tcgccacgac
      301 cctctcaatg actatcatat tcccgaagcc aatgtgcaaa agattataac ccatcatctc
      361 actccacgtt ctggacctgt tccctgatgc tctcaaggag gcccttggct tcaacggccg
      421 cccatcttac ctccgggtcc tggacctttg aggcaatagt gttgatctcc ctgttcatct
      481 cctggaggag aaaatccagg gtctttcccg agacctcatc attatgtacc aagccgagga
      541 atttatccac gtggctcgag gaccttgcga tctcctcggt gatatcccac ttgtcggaga
      601 ggagcactat ctcctgggcg atacggctct cgtcaacggc tggaccgtta gcgcccagta
      661 cctggctaac cctttccctg agggaaccga gggccttgtc ccgggcagtg ctccacttgc
      721 cctctatcac accggtgaca ttcctgaact ggatcatcga acccagaatt gcttcctgca
      781 ggtgcttacc ttcctttgcc ctcatggaat cccattcctc gatccctcta tccagaatgg
      841 tcccgacggc atctctgagg acgccttcca gttcgttgct gtcggtttcc tgcgaggaga
      901 ccgctcccgg gagggtgagc agaagttcca ggtccacctc tcccttcaat cccagttgat
      961 ctctgatgtc ctgcatttcc tgagcgtagc ctttcaggac ctccgactct atccttgagg
     1021 ccctgaccgc cgaatcccac gaaatgtcta cccttgcctg gatctttccc ctcctggaag
     1081 atgacctcaa cttctgctgg atcatcggct caaggtgtga gatctccctg ggaagtcgga
     1141 cggatatctc ctggtttcgg tggttgacgc tcgatatctc cagagcgaaa gtaccgagcc
     1201 ctgtcctctc ctctgtccgg ctgaaaccgg tcatactctt cagcaactgg cacactcctt
     1261 tccccggctc atggaaagca tctcctccac tacagaaagg agttccggga tcttgtcgcc
     1321 cctgagagca cttatgggga cgaccctgtc accggatctt cccgcaagcg ccctgatggg
     1381 ctctacagtt tcagggtcaa gcctgtctat cttgttcagg gctataatcc tcggcacagt
     1441 accggctcct atggaggcca gcacttcctc caccacttcc acgtcctttt cgtacctccc
     1501 tgaggacggg tccgctacca ggatgacaag gtcagcagcg gcgacctcct cgagggtggc
     1561 cctgaaggcg gtaacgaggt caggggggag gtctcttata aaaccgaccg tatcggcaaa
     1621 gagggccggg tctccgccgg gtagacggac cctcctgatg aagggatcga gggtggaaaa
     1681 gagcttgtcc gcaacgggga ggtcagagtc accggaaagt ctcctgagaa gcgtggactt
     1741 cccgctgttt gtgtaacccg taagggcgac cgtggcaaga ccggccctga tcctgtgttt
     1801 tctctgatcc ttcctcctct ctcttattgc ctctatcttc ctgttgatct ccctgatcct
     1861 tctctccagt tttcttctat gcctttcaaa ctccgtctca ccaggccccc tggtgccgac
     1921 acctcctccc gggcgggaca tctggagccc aagccccttg atgaaaggta tctcgtatcg
     1981 acagcgggcc agttctatct gcagctttgc ctcgccagtc tgggccctgt cctcgaagat
     2041 cttcatgatc accaggggtc tgtcccagac ggcaaggccg gtcaggcgcc ccagtgcggc
     2101 cgcctggcca ggcgaaaggc ttccgttgac aacaataagc cccgctccaa gatcccttgc
     2161 ctcggcaccg acaagttcgg cttttccgga acctatgaaa gaaccaggat caggccttga
     2221 gcgtcgttga atggaaattc ctgtagttgt tatcccgaga ttgccgagaa ggagcgacat
     2281 ctcctcaaga gagcccggaa cgtcgaggct gtcgtcctcg tcaagggcca ccagaaaggc
     2341 ctttcgtgag gatagctgcc gcaggtctat caaaaggacc tttcctccag ttctctcagg
     2401 gatctttcaa gattgtcggt cacagatctt ctcagttctt tcgaggagaa gccttccgta
     2461 ccgggcgatc ccggatatat gggccgaccg aaggagagta ctaccttgac gggcctgtaa
     2521 gtgcttgaac cgcgaggcat ggcctcaaaa gtcccatgga tataggcagg gataactgga
     2581 gccccggtct ttgctgcaag aagccctaca ccctcctcca ggggtttgag ccttccgtca
     2641 tgagacctct gcccctcggg gaagagaagt acgtcctcgc ccgactgtag tctctcgaca
     2701 aggaacttga gggcacctgc tgctccctgc aggtcttccc tcttggccgg tacagcgccc
     2761 agggccctga tcgccagacc caggggggca aaactgaaaa gttccgactt ggcaaggtat
     2821 ctcaatctcc tggggaaaaa ggcgcctatt acgataggat ccaggttgct gcagtggttg
     2881 gccgcaataa tgaccggggg acttgcggcc agcgccccgt caagccctat caccttcact
     2941 ctgttgtaga ccttcatcac tagcctgaag aacaaagcta tggtccagta gaagagagtt
     3001 ctcaagaata aacctccctt ggcctacttc aacaggccct ttggtcttcg atcacagcca
     3061 ggatcacgtt cacgacctct tcaggggacc tcttcgaagt atctatgata tgtgcgtctt
     3121 ctggaaccct caagggggag aactccctct cactgtcgag ctgatcccgt ctctggacct
     3181 gctttcttac ctcttcgagg gttatgttga cgccctttgc attcagctct ctccaacgtc
     3241 tggtcgccct ctcttcgagg ctggcgtcaa gatagaactt gatcggcgct tccgggaaga
     3301 cctcactccc catatctctt ccctcggcca ccattttcct tcctgaagct gcccttttct
     3361 gtatctgcag aagaaaactc ctgaccgtgt gaagagccga ataggctgat actgccaggt
     3421 cgatctccgg cgaccgtatc ttgtccgata catccacacc gtctataaat accctgccct
     3481 tttcaatgga gatagtgcat tcttccaggg catgggcaat atttccgcct tccgaaggcc
     3541 ggatggacct cccggaaaga aagtacgtta tcccccggta tattgccccg gtatcaagat
     3601 attcgtaacc aagcctcttc gctaccagcc ttgcgatggt gctcttgccc gcacccgccg
     3661 gaccgtcaat ggttattatg ttttcattgc atggatccgt ggtccccatc cggtcatcgc
     3721 ctccaatagc tatactgagc cgccgtacag gatcattttc ataaggacct cgccatcgag
     3781 gatcatgaac tctcccggtc tgagcctttt catatcaagt ttaccgatcc gggtcctgac
     3841 caacctcaca actttaaaac ctctcgtggc ggccattacc cttatctccc tcttcaaacc
     3901 ttccgaaagg gtgaccgaga tccaccgccc ctcaggtctt ctgggaatgg cgaccaccct
     3961 ttccggcctg acctttcttc cctctacctc agtgcctttc ataaaggatt ccagggcagc
     4021 atcatcagca gtggcgtcaa gcaggacctc gtaggtcttg aggatctggt ggctcggatg
     4081 aaggatccgc tgggccatct ctccgtcgtt ggtaagaagc agaagtccct cgcttccctt
     4141 gtcaagcctg ccgaccggga aaagaccctc tacagggatc tcggagggga gaagctcaag
     4201 gaccgttcgg gagaagctgt ccgagacgga acagacaaga cccctgggtt tgttcattgc
     4261 aatgtagagc tttggccgga tagaaatgac ctctccatcc agtttaacaa catcactttt
     4321 ggtatctatg gtcctggacg gatcggtaca gacctccagg ttgaccgata tcctcccggc
     4381 catgaccagc aggtcggcct ttcttctgga gcaaacaccg catgcggcta tgaacctgtt
     4441 gatacgaacc ccctgggtca tgttgccctt tcaccatctt cggggtactc gtcctgggac
     4501 agtatatcct gcatctcatc gagggtggga aggtccgata tagaaccgag cccgaaaagt
     4561 gagaggaagg agtttgtcgt tctgtaaaga aggggggttc ccgaaccctt tctcctcccc
     4621 gccaccctga tgaggccgtg cttcagaagg gtgtcgagca ccctttcgca ccttacacct
     4681 cgtatctcct cgatctcgga acgggtcacc ggttgggagt aggctatcac ggcaagggat
     4741 tccagggctg ccttgctcaa ccgtatcctc tgtgagagag ccacatctcg aaagagggtg
     4801 accacctcct cctgttcagg tgaggtggcc atcgaccagc ctccggctat gttcagaagg
     4861 caaaggccat ggcatttcct gtatctctcc tttatctcct ccaggaccct ctccaccgcc
     4921 tcctccgtta cgccagcgac cgaggccagc tccttaacgc caacaggctg aacggctacg
     4981 aaaagaactg cctccacgga ccttgcgatc ggagagagag gtgtctcaac cggatatcct
     5041 gacatcaccg aaaagctcct cctggtgaag tcttaccctt cccagcctgg ccatctcaag
     5101 tagcgccaga agggtcacca ccaacaaatg ccttgaacag ggagtcccaa ggacttcgct
     5161 tagcagaagt gcacctcttg atgatagcag catctctatc tcggacatcc ttccctcgac
     5221 ctgttcctct tccggcgtgg ctgccggaat gccgtcccac tcctcttcaa gacctgtcgc
     5281 aagggaccgg gtgccttcac gctt
//
LOCUS       DAOPKQ010000088         5304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_16820_length_5304_cov_4.163079, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000088 DAOPKQ010000000
VERSION     DAOPKQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16820_length_5304_cov_4.163079"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PK146_04190"
     CDS             <1..336
                     /locus_tag="PK146_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HPE91474.1"
                     /translation="VLDRQSVIAAACYLPLTENSNLSRWIGTRHRAALGVTEVSDAMA
                     LVVSEERGEVALAINGHLSRELKEGQIRRLLMHYFAHATGTGSAMEGVKERLGSVFSG
                     RGTENDEID"
     gene            320..1339
                     /locus_tag="PK146_04195"
     CDS             320..1339
                     /locus_tag="PK146_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPE91475.1"
                     /translation="MMRSIDRLLSSRLFLKVVSVLVAVLVWFYLASDRGTEIVRTVTV
                     PLEFLNVPVDMSVSSGVREVDIQVSGTRETAFSLTETIASQIDLKGLGPGSHRRPVQA
                     ILPSGLRLVEVSPPFVDLDLTRLGSRVLSVRLLVPDGLPPGYRLEEQRVAPMEVTVKG
                     PEHLLSSLENVWVVPTLEQLLQEKELTLPIVSSPDRDQRTPFMIEPAEADLSYSLVRG
                     FPRRVVPVRVELKGDPGKDFQVEAVVVDPPELTIQGPLESIERIEQVFLGPLDITGLS
                     ADVTRVVTVEDPADDVQVVEDPSVRVRILLAGRTERRLYAKVPVRLSGRSIYPGWRVE
                     PSRLA"
     gene            1336..1530
                     /locus_tag="PK146_04200"
     CDS             1336..1530
                     /locus_tag="PK146_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91476.1"
                     /translation="MILEGSPSDLDAAEVKGSPVDVYVDVTNLVSQKITVPVKTTLRS
                     KGVSLADIDPPNVTVYALTE"
     gene            1559..2965
                     /gene="glmM"
                     /locus_tag="PK146_04205"
     CDS             1559..2965
                     /gene="glmM"
                     /locus_tag="PK146_04205"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPE91477.1"
                     /translation="MQETSRKCRCLFGTDGVRDVANRGLMTPEMALRLGRSYALFLIE
                     RGVSRPVIAVGRDTRRSGPMIEMALVSGFLSAGAEVVSLGVIPTPGVSFAVRKLSCDG
                     GAVVSASHNTFEYNGIKFLGRDGCKLSDDDEAEIEEYLADVVFDDWRPTGSSVGSVAP
                     RTGILSEYTEHVVRTCGFGVDQELSIVVDCAHGAASEAAAMIFRRCFGRAIIISDRPE
                     RNNINDGCGVIHMENVASAVLSGGHSLGMALDGDADRVLFVDTKGRIIDGDIILWVLG
                     RWLHGEGRLVNGVVATVMSNKALEEKFAQDGIHLTRCPVGDRYVLDAMRSSGSSLGGE
                     QSGHVIASDFFPTGDGLSSGLLFLSACSRLGEDINTLVDRFGRYPQVLKNFHATDKES
                     FLGHPGVRGAIERACQELGELGRVLVRPSGTEPLVRVLVEAREDPVLRSVSEALFEEI
                     RRSGEEVLVRDLRGGEGC"
     gene            3007..3852
                     /locus_tag="PK146_04210"
     CDS             3007..3852
                     /locus_tag="PK146_04210"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPE91478.1"
                     /translation="MGTRFLPATKEIPKEMLPLIDRPLIQYGVEEAVRSGCGEIVFVT
                     GRGKRAIEDYFDRSIELEQILIGKGKADSAQLVREISQMADFAYVRQPEPLGLGHAVL
                     CGEPFCSGCYFGVILPDDVMVTPPGTPPVLGQLVQVHQRLGGSVVALEEVPPGETFRY
                     GIIRGEQVEDGLFRIEDLVEKPEPGSEPSRYAVMGRYVLSPGIFRYLRNSGVGTGGEI
                     QLTDAIRSLSEDEPVWGVMYKGERFDCGTMESWLLATLGMALKREDLRGIILSELERL
                     GVTGR"
     gene            4075..>5304
                     /gene="glmS"
                     /locus_tag="PK146_04215"
     CDS             4075..>5304
                     /gene="glmS"
                     /locus_tag="PK146_04215"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPE91479.1"
                     /translation="MCGIVGYVGRRNASDVLIDGLKRLEYRGYDSAGLALRTGDGLII
                     LKEVGKVADLERMLEEISPCSTQGIGHTRWATHGGVTQVNAHPHRDGSGKFAIVHNGI
                     VENYMELKEELEREGVEFLSETDTEVVVHLIATSFSQTGDILETLVNLQGRLRGSFAL
                     VVIQQDLPDRIYCVRKGSPLVLGSGDGESFCASDVPAFLPYTRKIQYLEEGDIAEISL
                     GGIRVWDENGCPVEKTVFTVDWDVSMTEKSGFSHFMLKEIHEQGMVLRSTLKGRIADG
                     RTAVDQELGWDAEMASKWDKVHLVACGTSYYSALVAERVLEKWTDLEIKVDVASEYRY
                     RHIKCDPSTLAVFVSQSGETADTLAAQRKARADGAHCLAITNVRGSTLAREVHSVMLL
                     KAGPEIGVAATKTFTGQM"
BASE COUNT         1111 a         1388 c         1717 g         1088 t
ORIGIN      
        1 gttcttgaca ggcagagcgt tatcgccgcc gcgtgttacc tgcccctgac agagaacagc
       61 aacctttcaa ggtggatagg gacccggcat cgtgccgccc tgggcgtaac ggaggtttcc
      121 gatgccatgg cgctggtagt ttccgaggag cggggagagg tcgcccttgc gatcaacggc
      181 catctttcaa gggagctgaa ggaggggcag ataagaaggc tcctcatgca ctacttcgcc
      241 catgcgacag ggacgggatc ggccatggag ggtgtcaagg agaggctggg ctcggtcttt
      301 tcagggaggg gaaccgagaa tgatgagatc gattgaccgt ctcctgtcct ccaggctctt
      361 cctcaaggtg gtttcagttc tggtggctgt gctggtctgg ttctacctgg cctctgaccg
      421 gggcaccgag atagtaagga ctgttacggt acctcttgag ttcctcaacg tccctgtgga
      481 catgtccgta tccagcgggg tccgtgaagt ggacatccag gtatcgggaa cgagggagac
      541 ggccttctcc ctgacggaaa ccatcgcgtc ccagatagat ctcaagggcc tgggacctgg
      601 atctcatcgc aggcccgtcc aggcgatact tccctcagga ctgaggttgg tcgaggtatc
      661 tccgcccttt gtcgaccttg accttacccg actgggatcc agggtgctgt ctgtcaggtt
      721 gcttgtgccc gatggccttc ctccgggata ccggcttgag gagcagaggg tcgctcccat
      781 ggaggttacc gtcaaggggc ctgaacactt gctctcttcc ctggagaatg tctgggtggt
      841 cccaaccctg gaacagctgc ttcaggagaa ggaactcaca ctgcccattg tgtcatcccc
      901 cgacagggac cagagaactc cctttatgat agagcctgcc gaggctgatc tctcctacag
      961 cctggtcaga ggtttcccca ggagggtcgt tcctgtcagg gtggaactga agggcgatcc
     1021 ggggaaggat ttccaggtgg aagccgtggt cgtggacccc ccggagctga ctatccaggg
     1081 gcctcttgag tctatcgaaa ggatcgaaca ggtctttctt ggccccctgg acataacggg
     1141 gctttcggcg gacgtcacca gggtcgtcac ggtcgaagat cctgctgatg acgttcaggt
     1201 cgttgaagac ccatcggtgc gggtgaggat actgctcgcg ggaaggaccg aaaggagact
     1261 ctatgccaag gtgcccgtca ggctttccgg tagaagcata taccccggct ggagggtcga
     1321 accctcgagg ttagcgtgat ccttgaagga tcaccgtcag accttgatgc agccgaggtc
     1381 aagggtagtc cggtcgatgt ctacgtggac gtaacgaacc tggtctccca gaagataacc
     1441 gtgccggtca agacaaccct tcgttccaag ggggtctcac tggccgatat agatccaccc
     1501 aacgtaacgg tctatgcgct taccgagtga gtcagggcgc agggaaagag gtgttccgat
     1561 gcaggaaact tccagaaaat gccggtgtct cttcgggacc gacggcgtaa gggatgtggc
     1621 caaccgtggg cttatgaccc ccgaaatggc acttcgcctg ggcaggtcct atgcactttt
     1681 ccttatcgaa agaggcgtat cccgcccggt cattgccgtc gggagggaca caaggcgttc
     1741 aggacccatg atcgaaatgg cccttgtctc gggattcctc tccgctggag cagaagtggt
     1801 ctccctgggg gtgatcccca ctccaggtgt aagcttcgcc gtcagaaaac tatcctgcga
     1861 cggtggtgcg gttgtaagtg cctcccataa caccttcgaa tacaacggta tcaagttcct
     1921 aggaagggac ggctgcaaac tgagcgacga cgacgaggcg gagatcgagg agtatctggc
     1981 tgacgtggtc ttcgacgatt ggagacccac aggttcgtca gtgggatcgg tcgccccgag
     2041 gaccgggatt ctttcggagt acaccgaaca cgtggtcagg acttgcggct tcggggtaga
     2101 tcaggaactt tctatcgtag tggactgtgc ccacggggcg gcttcggagg ctgcagccat
     2161 gatcttccgg cgctgcttcg gcagggccat catcatatcc gacagacccg aaagaaacaa
     2221 catcaacgac ggttgcgggg tcatccacat ggagaatgtg gcgtctgcag tcctttcagg
     2281 gggacattcg ctggggatgg cccttgacgg cgatgctgac agggttctct tcgtcgatac
     2341 caaggggagg atcatcgacg gagatatcat cctctgggtc cttggaaggt ggcttcatgg
     2401 cgagggcagg ctggtgaacg gggtagtggc gacagtgatg agcaacaaag ccctggagga
     2461 gaagttcgca caggacggta tccacctgac gcgctgtccc gtgggcgaca ggtatgtgct
     2521 cgatgccatg cgatcctcgg gttcttctct tggaggagag cagtcggggc acgttatcgc
     2581 aagcgatttc ttccccaccg gcgacgggct cagctccggt cttctgttcc tctcggcatg
     2641 ctcaaggttg ggtgaggata tcaacaccct ggtggacagg ttcgggaggt atccccaggt
     2701 tctgaagaat ttccatgcca ctgacaagga atcctttctc ggtcacccgg gggtgcgggg
     2761 ggcgatagaa agagcctgcc aggagctggg tgaactgggc agggtcctgg tcagaccctc
     2821 tggcacggaa cctcttgtgc gtgtcctcgt tgaagcgcgg gaagatccgg tattgaggtc
     2881 cgtatcggag gcgctcttcg aagagataag gagaagcggc gaagaggtcc ttgtccggga
     2941 tctcagagga ggtgaggggt gttgacgacg ggtgatgtca ggaaatgcct cttccccgtc
     3001 gcaggtatgg gtaccaggtt cctgcctgcc actaaggaga tccccaagga gatgctgccc
     3061 cttatcgaca ggccccttat ccagtacggg gtcgaggagg ccgtccgatc agggtgcggt
     3121 gagatagtct tcgtcacagg caggggcaag agggctatag aggactactt tgacaggtcc
     3181 atagagctcg agcagatact catcggaaag ggcaaggcag attctgcaca gcttgtaaga
     3241 gagataagcc agatggcaga tttcgcctat gtcagacagc cggaaccact agggctgggt
     3301 cacgcggtcc tgtgcggaga gcctttctgc agcggctgct attttggtgt gatcctcccc
     3361 gatgacgtaa tggtaactcc tcccggaacg cctccggtgc tcgggcagct tgtacaggtc
     3421 caccagaggc ttggtggatc agttgtggcc ctggaagagg ttcctccggg ggaaaccttc
     3481 aggtacggga ttattagggg tgaacaggtt gaggacggcc ttttcaggat agaggacctt
     3541 gttgagaagc ctgaacccgg cagtgagccg agccggtatg ccgtgatggg ccggtacgtg
     3601 ctctctccgg gaatattccg ctatctcagg aattcaggtg tgggtaccgg aggggagatc
     3661 cagctcacgg acgccatcag gtccctttcc gaggatgaac ccgtctgggg ggtcatgtac
     3721 aagggcgaga ggtttgattg cggtaccatg gagagctggc ttctggccac tctcggcatg
     3781 gccctgaagc gagaggacct gcggggcata atcctctccg agctcgaaag gctcggggtc
     3841 acggggaggt gacagtgtcg ggggagtaag aaagaggttg gcggctgacc aagcgccaga
     3901 actggccagg gccagttgac gagggccggg gagtatcgaa taattcggcg gatgccccgg
     3961 tggcctgaac ggtaaggcca tgaagccgtt cccacaaaac cagggagtga tctccgggac
     4021 aaaagggacg gcatccgttc tcttccagat cagaaaggcg gtgcccatac aaaaatgtgt
     4081 gggatagtag gttatgtcgg cagaagaaat gcctccgatg tcctgatcga tggactcaag
     4141 aggcttgaat atcgcggtta cgactcggcc ggtctggcac tgaggaccgg cgatggcctg
     4201 atcatcctca aggaggtcgg aaaggttgca gacctggaga ggatgctcga ggagatatcc
     4261 ccctgctcca cccagggtat cggacacacc cgatgggcga cccatggcgg cgtgacccag
     4321 gtgaatgccc atccccacag ggatggaagc ggcaagttcg ccattgtcca taacgggatc
     4381 gtagagaact acatggagct taaggaggaa ctcgaacggg aaggggtgga gttcctctcc
     4441 gagacggaca ctgaagtggt cgtccatctt atcgccacat cattctccca gacgggggac
     4501 atccttgaga cgctcgtcaa cctgcagggg aggttgagag gatcctttgc ccttgtggtg
     4561 atacagcagg atcttcctga caggatctac tgcgtaagga aaggttctcc ccttgtcttg
     4621 gggtccggcg acggagaatc gttctgcgct tcggacgtcc cggcatttct tccttacacg
     4681 aggaagatcc agtacctcga ggagggtgac atagccgaga tatcccttgg tggcattagg
     4741 gtatgggacg agaacggctg ccctgtcgag aagacagtct tcaccgtgga ttgggacgtt
     4801 tccatgacgg agaagtcggg gttctcacac ttcatgctca aggagatcca tgagcagggc
     4861 atggtactcc gatccaccct gaaggggagg atcgccgacg gaaggaccgc cgttgaccag
     4921 gaactgggct gggatgcgga gatggcctcg aagtgggata aggtccattt ggtggcctgc
     4981 ggaacctcgt actactcggc cctggtcgcg gaaagggtcc tggagaagtg gaccgacctg
     5041 gagatcaaag ttgacgtagc ctcggagtac cggtaccgac acatcaagtg cgacccttcc
     5101 acgcttgctg ttttcgtctc ccagtccggc gagacggcgg atacccttgc ggcccagcgc
     5161 aaggccaggg ccgacggagc gcactgcctt gccatcacca acgtgagagg ctcaacattg
     5221 gccagggagg tccacagcgt aatgctcctg aaggccggtc cggagatagg cgtggcagcc
     5281 accaagacct ttacggggca gatg
//
LOCUS       DAOPKQ010000089         5132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_17581_length_5132_cov_4.360055, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000089 DAOPKQ010000000
VERSION     DAOPKQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17581_length_5132_cov_4.360055"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PK146_04220"
     CDS             complement(<1..254)
                     /locus_tag="PK146_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91480.1"
                     /translation="MDVLIPVMAAAVRSGTPILYATLGEILTERSGVQNLGLEGLMLM
                     GAFSGFSVTYSTGDPWLGVLTAFFVGVAISAVHAFLSVSVG"
     gene            complement(256..1329)
                     /locus_tag="PK146_04225"
     CDS             complement(256..1329)
                     /locus_tag="PK146_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91481.1"
                     /translation="MRYRIVLRRRYKTGVFFEGILPVISIIAALLFAGGALLLFGVSP
                     LAAYKAMFRGALGSGYGLSEVIVKAIPLVISGVAVALAFRMKVWNIGAEGQIYLGALA
                     SAAAVRFLPSGNQVVMLLAMTAAAIIAGGAWGYIAGFLKSRWNVNEVITTLMLNYVGI
                     HLADHFIYGPWRDPSSLGFPMTPPFPPSARLPLFFGTRVHLGVLAAVLLVLLFRLVIR
                     WTRWGFEIRAIGDNPRAAEYAGINYGRNVALLMFISGAIAGLAGMTEIAGLQGRLQPG
                     FSPGYGYTAIIVAWLARLNPWGIMMVSFFMGVLLVGGDTIQVIMRLPLASVQVIQGCI
                     LFSVLGFEFFRNYKVLLLRRERE"
     gene            complement(1326..2885)
                     /locus_tag="PK146_04230"
     CDS             complement(1326..2885)
                     /locus_tag="PK146_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91482.1"
                     /translation="MRGIEKTFVGVKANSGVDMDLERGEVHTLLGENGAGKSTLMNIL
                     SGLYTADSGAIVINGEPRILRSPRDAIRAGIGMVHQHFMLVPTLSVWENVSLGLDDQP
                     FFLDKGRVIEKVGDIQKRYGINVDPMAPIWQLSIGEQQRVAIIKTLYRSADILILDEP
                     TSVLTPQETRALFRTIRQIKAEGHGVIFISHKLDEVMDISDRITVLRKGRKVASVNVK
                     DVTKESLAEMMVGRKVVFQIGKTLQNPGRVVLEARELVVRGDRGRVRVDHFSLDVRQR
                     EILGLAGVSGNGQGELCDALAGIRKTEKGSIKVDGCELAGRRTREFQKRGVSYIPADR
                     IGTGLVTGMNLRENIALRKYWRKEFTRFRLFIHWENIAGRTGQLVERYSVVNPGLGFP
                     ARMLSGGNLQKLMLARELSDDPRVVIAVQPTWGLDVSATLFVRETLLEQRDRGAGVLL
                     VSDDLEEILSLSDRIAVIHNGRLMGIVEDPDSVTEEQMGLMMAGTCLEEFSPPSGDGS
                     LGQEKEVTPSP"
     gene            complement(3016..4098)
                     /locus_tag="PK146_04235"
     CDS             complement(3016..4098)
                     /locus_tag="PK146_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPE91483.1"
                     /translation="MKKFALGLVCLMMVIGVSVAFAFQPVAEKDLKIGFVYVGPVGDG
                     GWTYMHELGRVDMEKAFPGVESFFVESVPEGPDSVRVMETFIRNGARLVFATSFGYMD
                     FVQEVAAKNPEVIFMHCSGYKTADNVGNYFGRMYQARYLSGLVAGKMSKKNVIGFVAA
                     NPIPEVIRGINAFTLGARKSNPDVKIKVVWLFSWFDPAREKEATLALIDAGADVIAMH
                     ADSGAAPQTAEEAGVYVVGYNNDMSHYAPTMHLTAPIWNWGMVYKHVVGQVTEGTWKS
                     SDIWWGLKEEFVGLAPISDAVPTDIVALVEAEKAKIIDGSWDVFHGPVKNQKGEVVVK
                     SGAKMTDGEMLGMNWFVEGVEGDIPK"
     gene            complement(4209..5030)
                     /locus_tag="PK146_04240"
     CDS             complement(4209..5030)
                     /locus_tag="PK146_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPE91484.1"
                     /translation="MKKKEFKLKGFSYPLTPLGRSSIVGPYPWHYGTEYLNILYRTDP
                     EVIASYLPYPLEPGEEPDLAYVAFSRWWSLWEEDSDMPWLNPERTQYLEAAIWTGCSF
                     EGRQGQICLHIWVDNDFSMARGWFMGFPKKLGQVFMTDYHPLNPGMARPGPGTRLKGI
                     ASAHGERLIEGEMEIVDSASPGELPRPMGLPLFHIRHFPSIEKGAPPSILELVSLGAE
                     NYRYGEIAWRGRGKLTFFPSEIEEHFALAPVEILGAFSFSSGYTFDGGRVLYSWV"
BASE COUNT         1162 a         1596 c         1281 g         1093 t
ORIGIN      
        1 cccaccgaaa cagagagaaa ggcatgtaca gcggagatcg caactcccac gaaaaaggcc
       61 gtcaggactc ccagccaggg atcccccgta gaataggtaa cggaaaaccc cgagaaggcc
      121 cccatcagca taagcccctc aagccccagg ttctgtaccc ctgatctctc ggtaagtatc
      181 tctccaaggg tggcgtagag gataggcgta ccgctcctga cggcggcagc cattacaggg
      241 ataaggacat ccatcctact cgcgctccct tctcaacagc aacaccttgt agttcctgaa
      301 gaattcgaaa ccgagtacgg agaaaaggat acaaccctga atgacctgca cgctcgcaag
      361 aggaagcctc attatgacct ggatagtgtc tccgccaaca agaagaaccc ccatgaagaa
      421 ggagaccatc atgatgcccc atggattcag ccgggccagc caggcgacta ttatcgcggt
      481 atacccgtaa ccgggagaaa aacccggctg aagtcgcccc tgaaggccgg cgatctccgt
      541 catcccggcc agccccgcta tggcacccga aatgaacatc agcagcgcca cgttccttcc
      601 gtagttgata cctgcatact ccgctgccct ggggttgtcc ccgatggccc gtatctcgaa
      661 accccacctg gtccacctga tcaccagtct gaacagaagt accagcagaa ctgccgccag
      721 gacccccagg tgcaccctgg tcccgaaaaa gaggggtaac ctggcagaag gggggaaggg
      781 tggggtcatg gggaagccga gactggaggg gtcacgccag ggcccgtaaa tgaagtgatc
      841 agcaagatga atgcctacgt agttaagcat cagggtagtt atgacctcgt tgacgttcca
      901 gcgggatttc aggaatccgg cgatataccc ccaggccccc cccgcaatta tcgcggccgc
      961 agtcatggca agaagcatga ctacctggtt cccggaaggg aggaagcgaa ccgctgcagc
     1021 agatgcaagg gctccaaggt agatctgccc ctcggcgcct atgttccata ccttcatcct
     1081 gaaagccaga gccactgcca cccccgaaat gacaagaggg atggccttga ctataacttc
     1141 gctcaaaccg tagccgcttc ccagggcacc cctgaacatg gccttgtacg cagcaagggg
     1201 cgagaccccg aagagaagca gtgcccctcc tgcgaacagc agcgccgcga tgatcgatat
     1261 gacgggaagg atcccctcaa agaacacccc cgtcttgtac cttcttctca gtactatcct
     1321 gtacctcatg gagagggggt gacctccttt tcctgcccga gactgccgtc acctgaagga
     1381 ggggagaact cctccaggca agtaccggcc atcatgagcc ccatctgctc ctcggtcacg
     1441 ctgtcgggat cctccacaat gcccatcagc cttccgttgt ggatcaccgc gatcctgtcg
     1501 ctcagggaga gtatctcctc caggtcatcg gagacaagca gcacccccgc ccccctgtcc
     1561 ctctgttcca ggagcgtctc ccttacgaac agtgtcgcgc ttacatccag cccccaggtc
     1621 ggctgaactg ctatgaccac cctcgggtcg tcggagagct ccctggccag catcagtttc
     1681 tgcagattgc caccggacag catgcgtgcg ggaaatccca atcctggatt tacaacgctg
     1741 tacctttcca ccagctgacc tgtcctgccg gcgatattct cccagtgaat gaaaagacgg
     1801 aacctcgtga actcttttcg ccagtatttc ctgagggcta tgttctcccg gaggttcatc
     1861 cccgtgacaa gaccggtccc gatcctgtct gcaggaatgt agcttacgcc tctcttctgg
     1921 aactccctgg tccgtcttcc cgccaattcg cacccgtcca ccttgatact gcccttttcg
     1981 gtctttcgga tgcctgccag tgcgtcacag agctctccct gtccgttgcc cgaaaccccg
     2041 gcaagtccca gtatctccct ctgcctgaca tcgagtgaga aatggtctac ccttaccctc
     2101 ccgcggtctc ccctgacgac cagttccctt gcctcaagca ccaccctgcc gggattctga
     2161 agggttttgc cgatctggaa gaccaccttc ctccccacca tcatctcggc aagtgattcc
     2221 ttggtcacat cctttacatt aacagaggcc accttcctac ccttccgaag aacggtgatc
     2281 cggtctgata tatccatgac ctcgtcaagc ttgtgggata tgaatatgac tccatgtccc
     2341 tcggccttga tctgacggat ggtcctgaaa agggcccttg tctcctgggg ggtgagaacc
     2401 gaggtcggct cgtcaaggat cagtatgtcg gcactgcggt aaagggtctt gatgatcgct
     2461 accctctgct gctctcctat ggagagctgc catattggcg ccatggggtc gacgttgatg
     2521 ccgtacctct tctggatatc cccaactttt tcgataaccc tccccttatc caggaagaag
     2581 ggctgatcat cgagaccaag ggaaacgttc tcccatactg acagggtggg aacaagcatg
     2641 aagtgctggt gtaccatgcc tatacccgcc ctgatggcgt cacgagggct cctaaggatc
     2701 ctcggctcac cgttgatgac aatggctccc gaatcggctg tatagagacc gctcaggatg
     2761 ttcatcaagg tactcttgcc tgcgccgttc tcgcccagaa gggtatggac ctcccctctt
     2821 tcgaggtcca tgtctacacc agagttggcc ttgacgccga caaaagtctt ttctatcccc
     2881 cgcatcgata caaggggcac ccggcctgcc gataccactt ccagtacctc caagggtttt
     2941 cttctacaag aaagattcta aaccatgaag agaaaaaaaa agggcgcgtg actcgcgccc
     3001 cctgaaaaaa gatctctatt tcgggatgtc tccctccaca ccctcaacga accagttcat
     3061 tccaagcatc tctccgtcgg tcatctttgc gccactcttg acgaccactt cgcccttctg
     3121 gttcttgaca gggccatgga atacatccca gctgccgtca atgatcttcg ccttctcggc
     3181 ctcgacgagc gccacgatat cggtcggaac agcatcagag ataggggcca gacctacaaa
     3241 ctcttccttc agaccccacc agatgtcgct ggacttccag gttccttctg tcacctggcc
     3301 cacaacatgt ttgtagacca ttccccagtt ccagatcggt gctgtgaggt gcatggtcgg
     3361 ggcgtagtgg ctcatgtcgt tgttgtagcc tactacgtag acacccgctt cttccgcggt
     3421 ctgtggtgct gccccggaat cggcgtgcat ggcgataacg tcagctccgg cgtcgatgag
     3481 agcaagggtg gcctctttct cccttgcggg atcgaaccag gagaaaagcc agacaacctt
     3541 gatcttcaca tcggggttgg acttgcgcgc acccagcgta aaggcgttga tcccccttat
     3601 gacctcgggg ataggattgg cggcaacaaa gccgatcacg ttcttctttg acatcttccc
     3661 tgcaacgaga cctgagaggt atcttgcctg gtacatcctc ccaaagtagt tacccacatt
     3721 gtccgcagtc ttgtaccccg agcagtgcat gaagatcact tcgggattct ttgcagccac
     3781 ttcctgtacg aaatccatgt atccaaagga ggtggcaaaa acgagcctgg cgccgttcct
     3841 gataaaagtc tccataaccc tgacggaatc gggaccctcg gggacggatt cgacgaagaa
     3901 gctctctact cccggaaagg ccttctccat atccaccctg cccaactcgt gcatgtaggt
     3961 ccatccgccg tcaccgacgg gtccgacata aacaaaaccg atcttcaggt ctttctccgc
     4021 cacaggctgg aaagcaaagg ctaccgagac tccgatcacc atcatcagac agaccagccc
     4081 aagagcaaac ttcttcatta gcaccagacc cccctgcaat ttgagtttcc attgccatgt
     4141 cactttacat tttgacgaaa aggacaataa atttcaaccc ccttttcggg gaagtttgaa
     4201 ataaggattc atacccagct gtacaggacc cttccaccgt caaaagtgta ccccgaactg
     4261 aaggagaagg ctcccaggat ttcaacgggc gcaagagcga aatgctcctc gatctccgaa
     4321 gggaagaagg tcagtttgcc acggccccgc catgcgatct ccccgtatcg gtaattctcg
     4381 gccccaagcg aaacaagctc caggatcgag gggggagcac ccttctcgat ggaggggaag
     4441 tgccttatat ggaaaagggg aaggcccatt ggtcggggaa gttcccccgg tgaagcacta
     4501 tcaactatct ccatctctcc ctcgatcagc ctctctccgt gagccgatgc tatccccttg
     4561 agccgtgtcc caggtccagg tcgtgccatt ccggggttca gggggtgata atcggtcatg
     4621 aagacctgcc ccagcttctt cgggaatccc atgaaccagc ccctggccat gctgaaatcg
     4681 ttgtcgaccc atatatgaag gcatatctgt ccctgcctcc cctcgaagct gcagcctgtc
     4741 catatggccg cctcaaggta ctgggtcctc tccgggttaa gccagggcat gtcgctgtct
     4801 tcttcccaca aggaccacca ccggctgaaa gcaacgtagg caaggtcagg ttcttctccc
     4861 ggttcaaggg ggtatgggag gtaggacgcg atcacttcgg ggtcggtcct gtagaggata
     4921 ttcaggtact ccgttccgta gtgccacgga taagggccaa ctatcgagga acggcccaaa
     4981 ggggtgagcg ggtaggaaaa ccccttcagc ttgaattctt tctttttcat cttttctcct
     5041 cccttgttca tcttccctgt acacggggga aaaacaaaaa aatggcggag acgtgtgggg
     5101 atcgaaccca ccggggccgg ccgtaccgac cc
//
LOCUS       DAOPKQ010000090         5114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_17654_length_5114_cov_4.844238, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000090 DAOPKQ010000000
VERSION     DAOPKQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17654_length_5114_cov_4.844238"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..558
                     /locus_tag="PK146_04245"
     CDS             118..558
                     /locus_tag="PK146_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPE91485.1"
                     /translation="MKDESHESLTEYATRLVYRSELTRMQLKRKILARGAMEIAADRI
                     CDEMQETGLIDDRRYCELFVSTHRELGFKRMRMELLKRGVERDLVDRMVPFDLEDEIE
                     RAEELIRHWGPEVDTKKTWGRLSRRGFTFSVIREAIRRACDSTS"
     gene            656..2185
                     /gene="rny"
                     /locus_tag="PK146_04250"
     CDS             656..2185
                     /gene="rny"
                     /locus_tag="PK146_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPE91486.1"
                     /translation="MLPVMLAAAFAVGISAGFLVYRYLSDKKLKGTREQSDQLLKDAF
                     QEADRRKREMLTEAREEMHRLRQDTEREIKERRAELQRAERRLEQKEENLDSKMETIL
                     HREEEFRRRQEEMSVRLEEVESVRRKQMERLEEIAGMSREQAKDVLLKEVEEEAAHLI
                     GLKLKELEEKARRESERKSREIVVSAIQRCAVDHTSEMSVSVVSLPSDEMKGRIIGRE
                     GRNIRAFETFTGVDLIVDDTPEAVTLSSFDPVRREVARLSLERLVLDGRIHPARIEEI
                     VEKAQKDVEEKILEAGEEAMLETGIKSMHPELIKLVGQLRYRSSYGQNALSHSLEVSH
                     LSGIMAAELGIDEHLARRAGLLHDIGKAVDHQVEGPHALIGRDLAKRFGEIPEVVNAV
                     AAHHEDQEMTSVYDVIVSAADAVSASRPGARRESLESYIKRLETLETLAKTFKGVTKA
                     YAIQAGREIRVMVAPNSLDDGAIHKLAYDIARKIESEMKYPGQIKVIVIRETRSVEFA
                     K"
     gene            2195..2968
                     /locus_tag="PK146_04255"
     CDS             2195..2968
                     /locus_tag="PK146_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPE91487.1"
                     /translation="MKVLFVGDIVGNPGRKALSGLLPRAKAEWGPFDLIIANVENAAG
                     GFGLTEKVMEELFRQGLDAMTSGNHIWDNREAFALLDAENRLVRPANYPPSTRGNGSM
                     VIEKKGIKLGILNLQGRVFMQPIDCPFRRADEELLRMEGLPVLVDVHAEATSEKRALA
                     LYLDGRVSAVLGTHTHVQTADEAILSGGTAFISDVGMTGGHGGVIGMEKDAIINRFLT
                     GMPTKFEVCKTDLKMDAVVVEIDGGTGRSLSIERLSLEM"
     gene            3368..4723
                     /gene="mgtE"
                     /locus_tag="PK146_04260"
     CDS             3368..4723
                     /gene="mgtE"
                     /locus_tag="PK146_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPE91488.1"
                     /translation="MRERLDRLYSHIERLLQEHRFPETKEALSTMEPADISEMLVGSP
                     RAKMVLIFRLLPKDLAIEVFEHMEGSGREELLASFTEEEAAEIIEEMSDDDRTALLDE
                     LPAKTVKKLMQHLSPEERKIANRLLNYPEDSAGRIMTAEYIGLKASMTVVDALQHIRR
                     QARSKETIYTCFVMDRERRLKGVVDLEDIILAQPETLISDLMDDDPVHVRTTMDQEEL
                     ARLMSRYDLHVIPVVDLEDRLVGIVTFDDVLDIVEEEATEDFERMAGIQPIDEKYLDT
                     GVFTLAGKRLTWLMVCILTQALTSSILKYHESSIQSVVALTFFIPFLIDSGGNTGTQS
                     STLMIRGMTLGEIEWGNIGRVFMREAFTGLMLGAALALFGVFRAITLGTGTDVAVVVA
                     VSLVAVVLIGNLAGVILPFAARALRFDPAIVSGPFITTVVDVVGLLVYFGIATRIMGL
                     G"
     gene            complement(4858..>5114)
                     /locus_tag="PK146_04265"
     CDS             complement(4858..>5114)
                     /locus_tag="PK146_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPE91489.1"
                     /translation="TLSVCDILGFDPLHMAGEGCAAIAVSGEDPDRALGLLRAHSLGE
                     RASVIGRVTEKHQGMVGIRTVSGGTRLVDRPVGELLPRIC"
BASE COUNT         1218 a         1265 c         1594 g         1037 t
ORIGIN      
        1 gatgcctttt ctcttgtacg gtcggatcca tctttatccg gtttccctga tcttcgcagg
       61 gaggtcttca ggaagctggg agccaaactc aggcttgccg ggacggcgtg agtggcgatg
      121 aaggatgaaa gccacgaaag cttgacggag tacgctacaa ggctggtgta cagatccgag
      181 ctgaccagaa tgcagctcaa aaggaagatc ctggccagag gagccatgga aattgcagca
      241 gacaggatct gcgatgaaat gcaggagacg ggattgatag acgaccggcg ctactgcgag
      301 ctcttcgtct ccacccatag agagctgggt ttcaagagga tgaggatgga actcttgaaa
      361 aggggtgttg agagggacct tgtcgacaga atggtcccct tcgaccttga ggacgagatc
      421 gaaagggcag aggaactgat ccgccattgg gggccagaag ttgatacgaa aaaaacctgg
      481 ggcaggctgt ccaggagagg ttttaccttc tccgttatca gggaagcgat ccggcgggct
      541 tgtgatagta cctcctaatc tgtagaatac atcaggacgc ttttttgcat attgcgactg
      601 ccgaatagat cgacaggtca cgaaacttga caacggaata aggagtgatg ttcagatgtt
      661 gcctgtaatg cttgcagcag ctttcgccgt cggcatttcg gccggcttcc ttgtgtacag
      721 atacctgagc gacaagaaac tcaaggggac cagagaacag tccgaccaac tcctgaagga
      781 cgcttttcag gaggccgaca ggcggaaaag agagatgttg acggaggcaa gagaggagat
      841 gcaccgcctc cgacaggaca cggaacgtga gatcaaggaa aggcgcgctg aacttcagcg
      901 cgcggaacgg aggctcgagc agaaggagga gaacctcgac tccaagatgg agaccatact
      961 ccatagggag gaggagttcc ggagaagaca ggaagagatg tcggtccgcc tcgaagaggt
     1021 cgagtccgtc cggaggaaac agatggaacg cctggaggag atcgccggca tgtcaaggga
     1081 acaggccaag gatgttctcc tcaaggaagt ggaagaagag gctgcgcacc tgatagggct
     1141 caaactcaag gagctcgagg aaaaagcaag aagggagtcc gaacgcaaat ccagggagat
     1201 cgtagtaagt gccatacagc gctgtgctgt tgaccatacc tctgaaatgt ccgtcagcgt
     1261 ggtatccctg ccatctgacg aaatgaaggg gagaattatt ggcagggaag gaaggaacat
     1321 ccgtgccttt gagaccttca caggggtcga ccttatcgtg gatgatacgc ccgaagcggt
     1381 gaccctgagc agtttcgatc ctgtgaggag agaggttgcc cgcctctccc tggaaaggct
     1441 tgtcctcgat ggaaggatcc atcctgcgag gatcgaggag atcgtagaga aggcacagaa
     1501 ggatgttgag gaaaagatcc ttgaagcggg tgaggaggcc atgctcgaaa cggggatcaa
     1561 gtccatgcac cccgaactga tcaaactggt cggtcagctg aggtacaggt ccagttacgg
     1621 acagaacgcg ctctcccaca gccttgaggt ttctcacctc tcggggatca tggcagctga
     1681 actgggtatc gatgaacacc tggccagacg ggcaggcctt ctccacgata tcgggaaggc
     1741 cgtagatcac caggtggaag gaccccacgc cctaatcggc cgggacctgg ccaagaggtt
     1801 cggggagatc cccgaggtag tgaacgctgt ggctgctcac catgaagacc aggagatgac
     1861 ctccgtttac gatgtcatcg tgtcagcggc tgacgcggtc agcgcctcta ggcctggggc
     1921 caggagggag agcctggaat cctacatcaa gaggctggaa acactggaga cccttgccaa
     1981 gaccttcaag ggtgttacca aggcctatgc catccaggca gggcgggaga taagagtaat
     2041 ggttgccccg aacagcctcg atgacggggc aattcacaaa ctggcctacg acatcgccag
     2101 gaagatcgaa agcgagatga aatatcccgg gcagatcaag gtaatagtta tccgggagac
     2161 gcgttccgtg gagttcgcca agtaggtgaa agcattgaag gtcctttttg tgggagatat
     2221 tgtcggcaat cccggaagga aggccctttc gggtcttctt cccagggcaa aagccgaatg
     2281 gggtccattc gacctcataa ttgcgaatgt tgagaatgcg gccggagggt tcggactgac
     2341 cgagaaggtc atggaagagc ttttccggca aggtctggac gccatgacaa gcgggaacca
     2401 tatctgggac aacagggagg cctttgcact tctggacgcg gaaaatcggc tggtcagacc
     2461 ggccaactat ccgccgtcaa cccggggcaa cggctcgatg gtgatcgaga aaaagggcat
     2521 taagttgggg atcctgaacc ttcaggggag ggtcttcatg cagcccatcg actgcccctt
     2581 ccgaagggcc gatgaggagc tcctgcggat ggaggggctg ccggtcctgg tggatgtaca
     2641 tgccgaagcg acatcggaga aacgtgccct ggcgctctac cttgacggaa gggtctccgc
     2701 agtgctcggc acccatactc atgttcagac cgccgatgag gcgatcctga gtggcggaac
     2761 ggccttcatt tcagacgtgg gtatgaccgg tggccacggt ggggttatcg gaatggagaa
     2821 ggacgccatc atcaaccgtt ttctgaccgg catgcccacc aagttcgagg tatgcaagac
     2881 cgatctgaag atggacgcgg ttgtggtaga gatcgacggg ggcacaggca gaagtctttc
     2941 gatagaaagg ctctctctgg agatgtaaag aaagtctgat ccagatgatc agagaccagg
     3001 gcggggcgcc cggagcgccc cgcccttttt gttgaccctg gagagctgtt gttatagcat
     3061 tgatcgagac tactgaccgg ggagatgtaa atggaagaaa gccaaagtag cggaccgcaa
     3121 tgcaaaagca caggtaagga aaaagagggg gtgaacgccg gagagagata gcggaagtta
     3181 tcctcacgca ccgcccctcc cggcagcggc aagaacgacc tgtccgccgc gcaagtcgcc
     3241 ctcgtgaaca taactgtact gagaccatac gcaggctgac ctgcctggcc tgaagtagcc
     3301 tttttggtgc tttcaagccc tgttgtgttt gttttgcagg aggccgcata ccgggggtgc
     3361 agccaccatg agagagcgtc ttgaccgtct atacagccac attgaaaggt tgcttcagga
     3421 gcaccgtttc cccgagacca aggaagcgct gtcgacgatg gagcctgccg acataagcga
     3481 gatgcttgtg ggtagcccgc gtgcgaagat ggtgctcata ttccgtctgt tgccgaagga
     3541 tcttgccata gaggttttcg agcacatgga gggttccggg cgtgaggaac ttctggcgag
     3601 ctttaccgag gaggaggcgg cggagatcat cgaggagatg tccgacgacg acaggacggc
     3661 ccttcttgac gaacttcctg cgaagacagt gaaaaagctg atgcagcatc tttcgccgga
     3721 ggagcggaag atagcgaacc gtctgctgaa ctacccggag gactcggcgg gcaggatcat
     3781 gacggccgag tacataggtc tgaaggcgtc gatgacggtt gttgatgcgt tgcagcacat
     3841 ccgcaggcaa gcccgtagca aggagaccat atacacctgc tttgtcatgg acagggagag
     3901 gcgcctgaaa ggcgtggttg accttgagga catcatcctt gcgcagcctg agacgctgat
     3961 atcggacctg atggacgatg accctgttca cgtaaggacc accatggacc aggaggagct
     4021 ggcgcggctg atgtcgcgtt acgaccttca cgtgattccg gtggtcgacc ttgaggaccg
     4081 tcttgtgggg atcgtgacct tcgacgacgt tctggacata gtcgaggagg aggccaccga
     4141 ggactttgaa cgcatggccg gtatccagcc catcgatgag aagtacctcg acacgggcgt
     4201 attcaccctc gcagggaaaa gactgacatg gcttatggta tgtattctaa cccaggcttt
     4261 gacatcgtcg atacttaaat atcacgagtc ttctatacag agcgtggttg ccctgacatt
     4321 tttcatacca tttctcatag acagcggagg caacacgggg acacaatcct ccaccctgat
     4381 gataaggggc atgaccctgg gtgagatcga gtggggcaac ataggcaggg ttttcatgcg
     4441 tgaggctttt acagggctta tgcttggcgc agccctcgct ctctttggcg tgttcagggc
     4501 cattactctc ggaacgggaa ctgatgtcgc agtagtggtt gccgtttccc tcgtggccgt
     4561 ggtgctgata ggcaaccttg ccggtgtcat tctgcccttt gcggccaggg ccctgcggtt
     4621 cgatccggct atagtttcgg ggccattcat tactaccgtc gtagacgtgg tggggctttt
     4681 ggtctacttc ggcatcgcga caaggataat ggggttgggg taggatgagg acagcgagat
     4741 gcgtgagaag taagcgtgag aggtttgttt ttgatccttc cgttcactgt tgaccgttaa
     4801 cgtccttgac tattctgttg cctgacgact gtagcctgcc gcctggtttt tgggttttca
     4861 acatatcctt ggaaggagtt cacctacggg tctgtcaacc agacgtgttc cccctgagac
     4921 tgtcctgatc cctaccatcc cctggtgctt ttccgtcaca cggccaatta cagaggccct
     4981 ctctccgagg ctgtgggctc tcagtagccc caacgccctg tcgggatcct cgccggatac
     5041 ggcaatggct gcgcatccct cccccgccat gtgaaggggg tcgaaaccca ggatgtcgca
     5101 aacagagagg gtag
//
LOCUS       DAOPKQ010000091         5023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18112_length_5023_cov_3.819243, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000091 DAOPKQ010000000
VERSION     DAOPKQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18112_length_5023_cov_3.819243"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK146_04270"
     CDS             complement(<1..406)
                     /locus_tag="PK146_04270"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPE91490.1"
                     /translation="MPPGEEKISGNLIYRGRILNLRVDSVKLPDGGRAMREVVEHAPA
                     VGVVSEADNGDLFLVRQFRYPAGEFLLEIPAGIVEPGEKAAETAARELQEEIGYIPGE
                     LNEVARFYTSPGFSNELIILFHARDLSHSPKEG"
     gene            complement(410..973)
                     /locus_tag="PK146_04275"
     CDS             complement(410..973)
                     /locus_tag="PK146_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE91491.1"
                     /translation="MSGFFRGMIAAGFGGQGIMVLGQLVAYTAISEGRHVTWIPSYGP
                     EMRGGTANCGVAISDEEIASPVVAEADVTVIMNNPSMERFEKSVKPGGLLIYNSDLVT
                     YQEPRKDVTVFPIPAHSVALSLGSDKVSNIVVLGAIVEATDLVGLEACIETIKDKLGK
                     RKPQFLPMNLEAFEKGREIARELLKKA"
     gene            complement(989..1735)
                     /locus_tag="PK146_04280"
     CDS             complement(989..1735)
                     /locus_tag="PK146_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE91492.1"
                     /translation="MKEIKIYERPKAWAKGVHTHYCPGCGHGITHRLICEIIDELGIQ
                     NDTIGTAPVGCAALMYDYIDVDFCEAAHGRAPALATGIKRTRPDKIVFTYQGDGDLAS
                     IGTAEIIHVANRGEKLTTIFINNAIYGMTGGQMAPTTLIGQKATTCPFGRDPQLAGYP
                     MRMCELLATLETPGFITRVSVARPKYIIEAKKAIKQAFQNQIEGKGYSFVEVLSNCPT
                     NWGMTPVQAFEWTESTMIPHYPLGVFKNFE"
     gene            complement(1758..2810)
                     /gene="vorB"
                     /locus_tag="PK146_04285"
     CDS             complement(1758..2810)
                     /gene="vorB"
                     /locus_tag="PK146_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPE91493.1"
                     /translation="MARVLMKGTEAIAEAAIQAGCNYFFGYPITPQNEIPEYMSAKLP
                     KIGGAYVQAESEVAAINMVMGGGSTGTKIMTTSSSPGISLMSEGISYIAGCEIPAVIV
                     NVMRGGPGLGGILPSQADYFQATKGGGNGDYRLLVLAPSTLQEAVEITQMAFDLALKY
                     RNPVMILADGFMGQMMEAVEIREHVSGEIPPRESWALGCMGERGGKRSILKSLYLNPE
                     DLEQHNIKLQKKYREMEKEVRFEAYLCEDAEILLSAFGTTARIARSAINNLRNEGIKV
                     GMLRPITLFPFPSRKLAELASGTKHVLVVEMNMGQMVEDVEAAVCGKAKVSFFGHAGG
                     IAPSVREIEDRIRAIL"
     gene            complement(2828..3049)
                     /locus_tag="PK146_04290"
     CDS             complement(2828..3049)
                     /locus_tag="PK146_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE91494.1"
                     /translation="MAKGRINVLEEYCKSCGLCVEACPVKVLRISEHINSKGYRPVEQ
                     FKEGCIGCRMCATTCPDAAIEVFRIEEEQ"
     gene            complement(3109..3879)
                     /locus_tag="PK146_04295"
     CDS             complement(3109..3879)
                     /locus_tag="PK146_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91495.1"
                     /translation="MKRPVEAHPFPRSLCEREWPKALAVTGALGSGKTEWVLNMAMAF
                     QGCGEEVTVADVDIINPYFCVRQVADSLREEGFHILTPPESTRWSDMPVISPQVSWAL
                     KDPRGRLLIDVGGDAAGGKALKQFSLQMKGSGYLLILVVNPFRPGSRETAGILDLMKG
                     MERISELQVGALVANPHLMDQTTAEDLLWGYRQVFDAGKTLGIEVLYGTVPPHLMSRR
                     ETLRKESPIELWPMVRHMMLPWEDGYMWTGAGDRSQGT"
     gene            complement(3876..4811)
                     /locus_tag="PK146_04300"
     CDS             complement(3876..4811)
                     /locus_tag="PK146_04300"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPE91496.1"
                     /translation="MTRKEPCWFAGSGRFAARCLEILAEKLPFSRVITALPKPAGRSL
                     GERGTPVDEKASELDLPVFRTGDVNVDRDILSLFGEERPFCIFVIDFSQLIGEPFISM
                     AGPGCLNIHPSLLPAYRGAAPIQRAIMNGDRETGVTVFRLAREMDAGPVLISSRVEIG
                     VNETFGDLMEILAVEGSRIALDGLELFRRGRISFKEQKHEEASFAPRISKQETELRWE
                     KPSRAVHDLVRSLNPYPGSFVTCGEKRIKIWRTRMRPENGVPGEILASTEENPVVACS
                     LGSVELVEVQEEGRRRTDGASWARGRSIRKGDLLK"
     gene            complement(4808..>5023)
                     /locus_tag="PK146_04305"
     CDS             complement(4808..>5023)
                     /locus_tag="PK146_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPE91497.1"
                     /translation="PGFYEKVARPERVVVEAKNKKGQPFRIEEKGFLARAMMHEIDHL
                     EGRLFFDRISPLKRKFLKRKLEKRVEK"
BASE COUNT          935 a         1559 c         1329 g         1200 t
ORIGIN      
        1 caccctcctt cggggagtgt gacagatccc tggcgtggaa gagtatgatc agttcgttgc
       61 tgaacccggg cgaggtgtag aaccttgcca cttcgttcaa ctccccgggg atgtagccta
      121 tctcctcctg cagctccctg gctgcggtct cggcagcctt ctcaccgggt tcgactatcc
      181 cggcaggtat ctccaggagg aattcaccgg cggggtacct gaactgcctg acaagaaata
      241 gatccccgtt gtcggcctcg ctgaccacgc caactgcagg ggcatgctcc acgacctccc
      301 gcattgctct tccaccgtcg ggaagtttga cgctgtcaac tctaaggttc agtatccgtc
      361 cacggtatat caggttcccg gagatcttct cctctcccgg gggcattgat caggccttct
      421 tcagcagttc ccgggcgatc tcacgaccct tttcaaaggc ctcgagattc atgggaagga
      481 actgcggttt tctctttccc agcttgtcct ttattgtctc gatacatgct tccaggccga
      541 ccaggtccgt tgcctccaca atggcaccca gcacgactat gttggagacc ttgtcgcttc
      601 cgagagaaag ggcgacgctg tgagctggga tggggaagac ggtaacatcc ttcctgggtt
      661 cctggtatgt gacgaggtcc gagttatata tcagcaggcc gcctggcttg acggacttct
      721 cgaaccgttc catcgagggg ttgttcataa ttactgtgac gtccgcctct gccaccaccg
      781 gcgaggcgat ctcctcgtca ctgatagcta ccccgcagtt cgcggtccca ccgcgcatct
      841 cgggtccgta ggaggggatc caggtgacat gacggccttc gctgattgca gtgtatgcta
      901 ccagctggcc caggaccatt atcccctggc cgccgaaacc ggcggcgatc attcctctga
      961 agaatccgga catatcattc tccctcccct attcgaagtt cttgaagacg cccagggggt
     1021 agtgcgggat catggtgctc tccgtccatt cgaaagcctg aacgggagtc atcccccagt
     1081 tggtcgggca gtttgagagg acctccacaa aggagtaccc cttcccctcg atctggttct
     1141 ggaaggcctg cttgatggcc ttcttggcct cgatgatgta tttgggcctg gctacagaga
     1201 ccctggtgat aaaacctggg gtttccaggg tcgccagcag ttcgcacatc ctcatgggat
     1261 aacccgcaag ctgcgggtcc ctgccaaatg ggcaggtggt ggccttctga cctatcaggg
     1321 tggtcggggc catctgtccg cccgtcatcc cgtagatggc gttgtttata aagatagtgg
     1381 tcagtttctc acccctgttg gcgacgtgga tgatctctgc agtgcctatt gaggcaaggt
     1441 caccgtctcc ctggtacgtg aagacgatct tgtcaggccg ggtcctcttg atccccgtag
     1501 ccagggcggg cgcccttccg tgggcggctt cgcagaaatc gacatcaatg tagtcgtaca
     1561 tcagggcggc gcatccgaca ggcgctgtcc ctatggtatc gttctggatc cccagttcgt
     1621 cgatgatctc gcagatcagc ctgtgggtaa ttccatgtcc gcaaccgggg cagtagtggg
     1681 tgtgtactcc ctttgcccaa gccttcggcc tttcatatat cttgatctcc ttcattggtc
     1741 cgacacctcc cctgcaacta aagaatggcc ctgatccggt cctcgatctc cctgaccgag
     1801 ggggcgatcc ctccggcgtg tccgaaaaag gagaccttgg cctttccgca aacggcagcc
     1861 tcaacatcct cgaccatctg ccccatgttc atctcgacca ccaggacgtg tttcgtcccg
     1921 gaggcaagct ctgcaagctt cctgctcggg aaggggaaaa gagttatggg tctgagcatt
     1981 ccgaccttga tcccttcgtt acgaagattg tttatggccg atctggcgat cctggcggtt
     2041 gttccaaagg ccgaaagcag gatctcggcg tcttcgcaca ggtaagcctc gaacctgacc
     2101 tccttttcca tctcccggta ctttttctgg agcttgatgt tgtgctgttc caggtcctca
     2161 ggattgaggt agagactctt gaggatggac cttttgcccc cgcgctctcc catgcatccc
     2221 agcgcccagc tctccctcgg gggtatctcg ccggagacgt gctccctgat ctccactgcc
     2281 tccatcatct gccccataaa accgtcggca aggatcatga ccgggttccg gtacttcagg
     2341 gcgaggtcga aggccatctg tgtgatctcc actgcttcct gaagggtgct gggggcaagg
     2401 acgagaagac ggtagtcgcc gttcccgccg cctttagtgg cctggaagta gtcagcctgt
     2461 gaaggcagga tgccgccaag gccggggcct cccctcatca cgttgactat gacggcgggg
     2521 atctcgcacc cggcgatata gctgataccc tccgacataa gggagatccc cgggctcgat
     2581 gaagttgtca tgatcttcgt acccgtggac cctcctccca tcaccatgtt tatcgcggcg
     2641 acctcgcttt cggcctgcac gtaggcaccg cctatcttcg ggagctttgc cgacatgtac
     2701 tccggtatct cgttctgggg ggtgatcgga tacccgaaga aataattgca ccccgcctgg
     2761 atggcggcct ctgctatcgc ttcagttccc ttcataagaa ctcttgccat ggataccacc
     2821 tccagcctta ttgttcctct tctatcctga acacttcgat agccgcgtca ggacaggtcg
     2881 tggcacacat tctgcagcca atgcagcctt ccttgaactg ctccaccggc cggtaaccct
     2941 tgctgttgat gtgttccgaa attctcagta ccttgaccgg acatgcctcg acgcaaaggc
     3001 cacaactctt gcagtattcc tcgagaacgt tgatacgtcc ctttgccatt tacccgcgca
     3061 ccctctttcc ttttgggttg taatagaggc cggatcaggg ttccggcctt aagtcccctg
     3121 tgacctgtct ccagcaccgg tccacatgta gccatcctcc caggggagca tcatgtgcct
     3181 gaccatgggc cataactcta tgggggattc cttccgtaaa gtttccctcc gggacatcag
     3241 gtggggcggt actgtgccgt aaaggacctc gatccccagg gtctttccgg catcaaagac
     3301 ctgtcggtag ccccagagaa ggtcctctgc tgtggtctgg tccatcaggt gagggtttgc
     3361 caccagggcc cccacctgca actccgatat ccgttccatg cccttcatga gatccagaat
     3421 gcctgcggtc tccctgctgc cgggcctgaa ggggttgacg accaggatca ggaggtaacc
     3481 cgatcccttc atctggagcg agaactgctt caaggccttc cctccggcgg catctcctcc
     3541 cacgtcgatg agaagcctcc ccctggggtc cttgagcgcc caggagactt gcggggagat
     3601 gacgggcata tcggaccacc tggtgctctc cggcggggtc agtatatgga aaccctcttc
     3661 tctcaggcta tcagccacct gtcttacgca gaagtagggg ttgattatat ccacgtcggc
     3721 cactgtaacc tcttcgccac atccctggaa ggccatggcc atgttcagta cccattcggt
     3781 cttccctgaa ccgagggctc cggtaacggc aagggccttg ggccattctc tctcgcacag
     3841 agagcgcgga aaggggtggg cttccactgg tctcttcact tcaggagatc ccccttccgg
     3901 atcgagcgcc ccctggccca gctcgcgcca tcggtacgcc ggcggccttc ttcctgaacc
     3961 tcgaccagct cgacagaacc cagactgcaa gccactaccg ggttttcctc ggtggaagcg
     4021 aggatttcgc ctggaacgcc attttcgggc cgcattcgtg tcctccatat tttgatccgc
     4081 ttttctccgc aagtgacaaa agatcccgga tatgggttca gcgaccttac aaggtcgtgg
     4141 accgctctgg aaggtttttc ccatctcagc tctgtttcct gcttcgagat cctgggtgcg
     4201 aaagaggctt cctcgtgctt ctgctccttg aaggaaatcc ttcccctgcg gaataattct
     4261 aaaccatcca gggctatgcg gctaccttcc acagcaagta tctccatgag gtcaccaaag
     4321 gtctcattga cacctatctc caccctggag gatatgagca caggccctgc atccatctct
     4381 cttgccagtc tgaagacggt cactcccgtc tctctgtccc cgttcattat tgctctctgg
     4441 atcggggctg caccccgata ggcgggcagt agcgaggggt gtatgttcag gcacccgggt
     4501 ccagccattg aaatgaaggg ttcacctatc aactgggaga agtcgatgac aaagatacag
     4561 aagggtctct cttccccgaa aaggctcagg atatccctgt ccacgttgac atcccctgtc
     4621 cgaaagaccg gaaggtccaa ctccgaagcc ttctcgtcaa ctggagtccc tctctcccca
     4681 agggaacggc ccgcaggctt ggggagggct gtgataaccc tgctgaaggg gagcttttct
     4741 gccaggatct ccaggcagcg ggcggcgaac ctccctgacc cggcgaacca gcaaggctcc
     4801 ttccgggtca tttttccacc cttttctcaa gcttcctctt gaggaacttt cttttcaggg
     4861 gggagatccg gtcgaagaaa agcctgccct caaggtggtc tatctcgtgc atcatggctc
     4921 ttgcaagaaa gcccttctcc tctatcctga agggttgccc cttcttgttc tttgcttcca
     4981 ccaccactct ctccggcctg gctaccttct cgtagaaccc ggg
//
LOCUS       DAOPKQ010000092         4975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18367_length_4975_cov_5.608130, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000092 DAOPKQ010000000
VERSION     DAOPKQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18367_length_4975_cov_5.608130"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..433)
                     /locus_tag="PK146_04310"
     CDS             complement(194..433)
                     /locus_tag="PK146_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91498.1"
                     /translation="MKGVAFFTADRNEEVSVQIFRDTMSGEPLLALDLKFPEIGYHLT
                     DPGRDVHSAAVDTAAVALCFRGSGVAGVRSCRWYT"
     gene            complement(881..3640)
                     /locus_tag="PK146_04315"
     CDS             complement(881..3640)
                     /locus_tag="PK146_04315"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPE91499.1"
                     /translation="MAEQAFYRMTAKETLEALESGEKGLTRADQGKRREKYGPNDITT
                     DVSTPKWLLFLSQLKDLLVLVLIFAGLISFAIGSLRDGTVMFVIVLLNATIGFVQEYK
                     AGKILEKLKDLIRSPARVRLDGELAEVPQSDLVPGDIVQIDAGDKIPADIRVMEAFNL
                     RVNEFSLTGESTSQEKKSNVIDEDVILADRENMLYMGTSVATGSAVGVVVRTGMNTEM
                     GKIANMTQDTVAVRSPLQVELGVLAKWLTVIVAFISAGLFAVALWQGFGFFMSMVYAL
                     GVAVAAVPQALPAQVTVALSVTSKRLADRKAIVKNLPSVETLGSTTVICTDKTGTLTK
                     NEMTVTRLWFDGESFTVTGIGYEPEGQITDENGEPLSPERVDEVEIMLDASTMASNAE
                     IHPPDEDHHGWYPVGDPTEAALITLSTKAGTRSPQEDEENPELHEFPFDSERKRMSSV
                     RQFEDRLELAMKGATDSVLSVARFIYRDGRSVPISDEDRRRIKELSEAWSKEALRVLA
                     IAFRPLDDSGDDYVMEEVEKDVVFLGLVGMIDPPKEGVREAVASCRQARIRIIIMTGD
                     HAITAQAVGKEIGLTDGEEPPVFTGAEMEAMDDRELTKVLMASESMIFSRVAPEDKLR
                     IVKLLESQGEVVAVTGDGVNDAPALKRAAIGVAMGQTGTDVAKEAAEVVLLDDSFPTL
                     VEAVREGRTIYSNLRKTVLASLTTNMAEMAVVLLGLAAVAAGNWAIPILAIQILAIDL
                     LAEIMPLTALTFDPPGDNIMQMPPRDRNTHILDIWSSLEVLFLGSVIGLLAFLNFGLF
                     MSRQGVLISPDMMGSPLFGRATAITFLTIVFCQFVNILSRRYETISFFSRNILSNRIL
                     LGSIALSIFTVLFGIYGPGISDFLAFSGPDLTDWLYVLGATASYLAVFETLKFGKRLR
                     SKQTI"
     gene            complement(3936..4023)
                     /locus_tag="PK146_04320"
     tRNA            complement(3936..4023)
                     /locus_tag="PK146_04320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3987..3989),aa:Ser,seq:gga)
     gene            complement(4043..4135)
                     /locus_tag="PK146_04325"
     tRNA            complement(4043..4135)
                     /locus_tag="PK146_04325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4099..4101),aa:Ser,seq:cga)
     gene            complement(4151..4624)
                     /gene="tadA"
                     /locus_tag="PK146_04330"
     CDS             complement(4151..4624)
                     /gene="tadA"
                     /locus_tag="PK146_04330"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPE91500.1"
                     /translation="MTENRRSVPEEHEPFMRIAMKEARKGASVGEVPIGAVVVLDGSV
                     IGRAHNRREGLKDPSAHAEILALREAAGYLGRWVLEGCTIYVTLEPCPMCAAAIVQSR
                     LERIVYGARDLRWGACGTLYDIPRDPRLNHRCDVTGGILSEECAKMLREFFLPLR"
     gene            complement(4621..>4975)
                     /locus_tag="PK146_04335"
     CDS             complement(4621..>4975)
                     /locus_tag="PK146_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91501.1"
                     /translation="RSVKARLDLERDVFIQLISRKSWGLGLVLGWPGRSQSGTDFFER
                     FHPGELKGDVFLVSDGKEAAHFYLETPEPRAGLFGPSPEKGQTGRVILGVSALGMSLA
                     LLWFLVYLGRRRERP"
BASE COUNT         1073 a         1531 c         1271 g         1100 t
ORIGIN      
        1 gaggatcgct gatctgttgt caccctttgg ctcggcttta cccggaacca tcattgatct
       61 tgtctcacag ccattacggc ctgaacgaaa aaggaacagc cttcacccct gtcggaatat
      121 ggggtcattt gaaaactatc tccgtcgaag gtgccgaaga aggtcgggca agtgggatta
      181 tagcccacat cccttacgta taccagcggc agcttcgaac gcccgccacg ccactgcccc
      241 tgaagcacaa ggcgacggct gcggtgtcca ctgccgcaga atgaacatcc cgacccgggt
      301 ctgtcaggtg atacccgatc tccggaaatt ttagatccaa agcaagaagc ggctcccctg
      361 acatggtatc cctgaatatc tggacagaga cctcctcgtt gcgatcagcg gtgaagaaag
      421 ccacaccttt catgttctct tcctgatccg gccgaaacct ggcgacagcc caaggatcct
      481 cgccttcaaa accgttaaga aggtagctga actctcccag gtcgataatg gacctttaat
      541 caattttctc ctcttcaaag atccttgaaa gggcacctgg acgggtctgt tactccgagg
      601 cgtgccggag gccgctcccc cgatccggga ccagtccgga gacaaggggc ttcaggtgga
      661 ccaggtcatc gcaagcgacc cacaagaatc cttccccgtg aaaagaatac cccacgggat
      721 ccttcaccat ccaggcgccg aacgcttttg cctttaacgg acaggcgcct gtggtcatcc
      781 cattcgacaa cggctacgca atgtgtatag aggaccgtgc acaacccctc tgtaaggaag
      841 aatcttccgt cgggaaatat gcggtatccg gctccttgcg ttatatcgtc tgtttgcttc
      901 gtagcctttt cccaaacttt aacgtttcaa acacggcaag gtacgaggcc gtcgctccca
      961 gtacatacaa ccagtcggtc aggtccggac cgctgaaggc aagaaaatcg cttattcccg
     1021 gaccgtatat tccgaaaagg accgtaaaga tggacagggc tatcgaaccc aggaggatcc
     1081 tgttgctaag aatgttcctg ctgaaaaagc ttatggtctc gtatctgcgg gagagtatgt
     1141 ttacgaactg gcagaatacg atcgtaagaa aggttatcgc tgtcgccctt ccgaacaggg
     1201 gagaccccat catgtcggga gagatcagga caccttgcct tgacatgaac agcccgaaat
     1261 tcagaaaggc cagcaggccg atgacggacc ccaggaagag cacctccagg gagctccaga
     1321 tatccagaat atgagtgttc ctgtcccgcg gaggcatctg catgatgttg tctccgggcg
     1381 gatcgaacgt gagagcggta aggggcatta tctcagccag caggtcgata gcgaggatct
     1441 ggatggccag gatggggatc gcccagttcc ctgctgctac cgccgcaagc cccagcagca
     1501 ccacggccat ttcagccatg ttggtcgtca gggaggcaag tacggtcttt cttagattac
     1561 tgtagatcgt acgtccctcc cggacagcct ccacaagcgt cgggaagctg tcatccagca
     1621 gcacgacttc cgctgcctcc ttggccacat ctgtccccgt ctgccccatg gccacaccta
     1681 tcgccgccct cttcagggcc ggcgcatcgt tgacgccgtc acccgtcacg gcaacgacct
     1741 ctccctgtga ttccagaagt tttacgatgc gaagcttgtc ctccggagcc accctggaaa
     1801 agatcattga ttcagaagcc atgaggacct ttgtcagttc ccggtcatcc atggcttcca
     1861 tctcggcacc cgtaaagaca ggaggctcct ccccgtcggt aaggcctatc tctttaccga
     1921 cagcctgcgc ggttatcgcg tggtcacctg tcatgatgat gatacgtatc ctggcctgcc
     1981 ggcagctcgc cacggcctct cgaacaccct ccttgggcgg gtctatcatc ccgaccaatc
     2041 cgaggaacac aacgtctttt tccacctctt ccatcacgta gtcgtcacca ctgtcatcaa
     2101 gggggcgaaa cgcaatggca aggaccctta aagcttcttt tgaccaggcc tcactgagtt
     2161 ctttaattct cctccggtct tcgtcactga tcggaaccga tctgccatcg cggtatatga
     2221 accttgccac tgagagaaca ctgtccgtag cccccttcat ggcgagctca aggcggtcct
     2281 cgaactgacg aactgaactc attcgcttcc gctcggaatc gaacgggaac tcatgcagtt
     2341 cagggttctc ctcgtcctct tgcgggctgc gggtccccgc ctttgtggag agcgttatca
     2401 gggcagcctc ggtcgggtca cctacgggat accagccgtg gtgatcttca tcgggcgggt
     2461 gtatctcggc atttgatgcc atcgtggagg catcaagcat gatctccact tcgtctactc
     2521 gttccgggga caggggttcg ccattttcgt ccgtgatctg cccttccggt tcatagccga
     2581 tgccagtgac cgtgaagctc tccccgtcga accagagcct ggtaacggtc atttcgtttt
     2641 tcgtaagcgt tccggtcttg tctgtgcaga tcaccgttgt tgatccgagg gtctccacgg
     2701 agggcagatt cttgacaatg gccttgcggt cggccagcct cttgctggta acggacagcg
     2761 ctaccgtaac ctgtgccgga agggcctggg gcactgcggc gaccgccaca ccaagggcat
     2821 agaccatgct catgaaaaaa ccgaaccctt gccagagcgc gaccgcaaag agcccggcac
     2881 tgataaatgc aacgataaca gtgagccatt tggccaggac ccccagttcg acctggagag
     2941 gcgacctgac tgccaccgta tcctgggtca tgttagcgat cttccccatc tccgtgttca
     3001 tgcctgtccg gacaacgacc ccgacagcac tcccggtggc cacggacgta cccatgtaga
     3061 gcatgttctc cctgtcagcc aggatcacat cctcgtcgat aacattgctt tttttctcct
     3121 gcgatgtgga ttcccctgta agcgagaact cgtttacgcg gaggttgaag gcctccataa
     3181 cccggatatc cgccgggatc ttgtcccccg catctatctg gactatatcg ccaggcacaa
     3241 ggtccgactg gggtacttcc gcaagttctc catccagcct tacccttgcg ggtgacctga
     3301 taaggtcctt cagtttctcg aggatcttgc cggccttgta ctcctggaca aaacctattg
     3361 tcgcgtttaa caacactatt acgaacatca cggtgccgtc cctcaaactg ccgatggcaa
     3421 aggagatcag acccgcaaag atcaggacga gaacaagaag gtctttcagc tgggaaagga
     3481 aaagaagcca cttgggggtt gatacgtcag tcgtgatatc gttggggccg tatttttcac
     3541 ggcgctttcc ctggtcggcc cgggtaagcc ccttctcgcc ggattcgagg gcttcaaggg
     3601 tctcttttgc tgtcatcctg taaaaagcct gttccgccat aaaatcctcc tgtctccaac
     3661 cctttggagg gtcagatcct gatctggccg ctaacaattc atgagacccg gcgacccttt
     3721 acctgtcatt gttgatgaca gatcttcttg ttgtttaaag cttcttcatc tatattaatg
     3781 caaatccctc atcctggaga ggcaatattc tctttccatt actcttggaa caacaaccaa
     3841 aacaaaagac ctccggagcg acagaccgat ctccagaggc cttaagagca tgaaaaagcg
     3901 agaccccgga tttttccggg gtctcgctga ttgtctggcg gagaaggagg gattcgaacc
     3961 cacgggacgt tgccgcccag acgctctcca agcgcccgcc ttcgaccact cggccacttc
     4021 tccatttgcg gtatttatta tatggcggag agagtgggat ttgaacccac gagacggctc
     4081 atcaccgcct agacgatttc gagtcgcccg ccttcgacca ctcggccatc cctccaccct
     4141 ttccttcccc tcagcggagg ggcaggaaga actcccggag cattttagca cattcttcag
     4201 agaggatgcc acccgtcaca tcgcaccggt ggttgagcct ggggtccctg ggaatatcgt
     4261 acaaagtgcc gcaggcaccc catcggagat cccttgcgcc gtagacgatc ctctccagcc
     4321 gtgactggac tattgcagcg gcgcacatcg ggcagggctc gagggtcacg tagatagtgc
     4381 acccttcgag aacccacctt cccaggtatc cggcggcctc cctcagtgcg aggatctcgg
     4441 catgggccga gggatctttc aggccctccc tccggttgtg cgcccgacca atgacagaac
     4501 cgtcaaggac cacgactgct cctatcggga cctccccgac ggaggcaccc ttacgggcct
     4561 ctttcatggc gatcctcatg aagggttcat gttcctcagg gaccgacctc ctgttttctg
     4621 tcatggccgc tctctcctcc tgccgagata gacgaggaac cacagcaagg caagtgacat
     4681 tcccagcgct gacacgccga gaataacccg tcctgtctgg cccttctccg gggagggccc
     4741 gaacaggccc gccctcggtt ccggggtctc cagatagaag tgcgccgcct ctttcccatc
     4801 cgataccagg aagacatccc ccttcagctc tcccgggtga aatctctcaa agaaatcggt
     4861 gccggactgt gacctgccgg gccagcccag cacaaggccg agtccccagc tcttgcgtga
     4921 tatgagctgg ataaagacat ccctctcgag gtccagcctg gccttcacgc tcctt
//
LOCUS       DAOPKQ010000093         4927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18581_length_4927_cov_5.806445, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000093 DAOPKQ010000000
VERSION     DAOPKQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18581_length_4927_cov_5.806445"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK146_04340"
     CDS             complement(<1..406)
                     /locus_tag="PK146_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91502.1"
                     /translation="MRILIAPEVFEKWPDYRRYVVVGKGLDNADEDTSLLALLREAES
                     GVRNDPELENYKEYPLIASWRSVFEDMGLNPNKFPPSIANLIKRTRSGKDLPFINRLV
                     AIFNIISLKYRIPCGGENLVAFCGTQPEVPLQA"
     gene            487..1440
                     /locus_tag="PK146_04345"
     CDS             487..1440
                     /locus_tag="PK146_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPE91503.1"
                     /translation="MREERPEGNRKALFFFCVGEGYDHVSRLVFDKVVELFNPRETGI
                     SVDGFPILEHVDEKGDRFCFMRQENLVSYDFIRYLPILREHFLDFDLAAEVNWHEGAN
                     APDRVITVHTVGDVDAGVFTPAVPGLMRGLLHAMEKERARARIDDFRVVTEATHWTGT
                     FRGQNPALLKEFPVPTLDIEIGSTPSSWEDPAAVEVLAKSLVRAFSPFKRLFNMLCVG
                     GIHFERSFSEAALNSDFPFGVSHILPNQWIVSGNYASEEGFEKLRSAALSINGGVDAL
                     VYHEGIKSPFREQCRRLGEELGIPVLKHKALRKPEDLCSPT"
     gene            complement(1463..2149)
                     /locus_tag="PK146_04350"
     CDS             complement(1463..2149)
                     /locus_tag="PK146_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE91504.1"
                     /translation="MNNRTIEELAGRMTGDTSAPHHIANVLREAIYRGILEEGKPLHQ
                     TQIAHRLGVSPIPLREALRLLETEGLVEFRGYRGAFVTELSLEEARELYEMVAALETS
                     LMRIAFPGITRKIIQDAREVLEKMEREQDCIRWRDLNFLFHTLFYEPADRPLTLDMVA
                     RLRQRTDRTIRTHLGSMRSESERQHRDILEAVTAGDLERSVFALAHHLAYTSNDLQSW
                     MRDEQSGRKR"
     gene            2326..3501
                     /locus_tag="PK146_04355"
     CDS             2326..3501
                     /locus_tag="PK146_04355"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPE91505.1"
                     /translation="MKFLTEDLEAMKRDGLYGTIRTIESPQGAWVKIAGKDYINLCSN
                     NYLGLCNDPRLVKKVKDYVDKYGIGPGAVRTIAGTMIPHIELERRLAEFKGADAAIVL
                     QSGFCANLAVIPTLVPSADDTIYSDSLNHASIIDACRLSKAKVVRYEHSNLDDLTAKL
                     EEFKGAPGKHLLVTDGVFSMDGDIAKLPEIVEICEKYGVIVVVDDAHGEGVLGRGGRG
                     IVDHFGLHGKVDVEVGTMSKAFGVMGGVIAGSTTLIDYIRQKARPNLFSSALTVPDIA
                     ANLASVDILEESEELVTRLRENGDFLKMELSAIGFNTGNSETPITPVIIGEAQDAKTF
                     SAKLFENGVFATAIVFPTVPKGTARIRCMVSAAHSRDDLNLAVERFAKVGREMGIIL"
     gene            3498..4439
                     /locus_tag="PK146_04360"
     CDS             3498..4439
                     /locus_tag="PK146_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPE91506.1"
                     /translation="MKRILVTGSNGQIGVELVPYLRKNYGAENVLATARRKVSGPVSE
                     GGPFEILEVRDGKTFSELMDSFKADAIIHLAGILSARGEACPQESWDINAGGCFTALE
                     CAREHGASFFFPSSIAAFGPSTPADSTPQETIQRPTTIYGVAKVTGELLCDYYHLKFG
                     LDTRGLRFPGLISYEALPGGGTTDYAVHIYYEAVSKGKYTSYIAPGTCMDMMYMPDAL
                     ESVVQLMEADPSKLVHRNAYNISAMSFDPDQITASIKKVIPSFEISYDVDPVRQGIAE
                     SWPNSLDCSAAKAEWGFAPKYDLDAMTVDMLERVGKK"
     gene            complement(4644..>4927)
                     /locus_tag="PK146_04365"
     CDS             complement(4644..>4927)
                     /locus_tag="PK146_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91507.1"
                     /translation="RQHLMERSFARGKRYGYDRARWRGLWRMSIQEYLTCAVQNIQVL
                     LKGGGRSAKDPAVIARILEPGLSRVCFLFSKILRRSCLAYIEQPVTCPA"
BASE COUNT         1086 a         1369 c         1427 g         1045 t
ORIGIN      
        1 gtgcctggag ggggacctca ggctgggtcc cgcaaaaggc gacgaggttt tcgccgccgc
       61 aggggatgcg atacttcagg cttatgatgt tgaagatagc aaccagcctg tttatgaacg
      121 gcaggtcctt gccggaacgc gtccgcttga tgaggttggc aatggacggc ggaaacttgt
      181 tgggatttag tcccatatcc tcgaagacgg aacgccacga cgcgatgagc gggtactcct
      241 tgtagttctc aagttcgggg tcgttcctta cccccgactc ggcttccctg agcagggcaa
      301 gaagggaagt gtcctcatcg gcgttgtcca gcccctttcc caccacgaca tagcgccggt
      361 aatcgggcca cttctcgaaa acctcaggtg cgataagtat cctcatgggg agactctcct
      421 ttcaggcacg gtctcttctt gggtatgcca tgtataataa caggactttc aaagggaggg
      481 taaggaatgc gagaggagcg ccccgaagga aacaggaaag cccttttctt cttctgcgta
      541 ggagaaggct atgatcatgt ctcaaggctg gtcttcgaca aggtggtgga gctgtttaat
      601 cccagagaga ccggaatatc ggtcgacggc ttccccatcc tggagcacgt ggatgaaaaa
      661 ggcgacaggt tctgcttcat gcgccaggaa aaccttgtga gctacgactt catacgctat
      721 ctgcccatcc tgagagagca cttcctcgac ttcgaccttg ccgccgaggt caactggcac
      781 gagggtgcca acgcaccgga cagggtcatt actgttcaca ctgtggggga cgtggacgcc
      841 ggagttttca cccctgctgt tccgggtctc atgaggggcc tcctccatgc aatggaaaag
      901 gagagggccc gggcgaggat cgatgacttc cgcgtggtga ccgaggcaac tcactggacg
      961 gggaccttca gggggcagaa tcccgcgctt ctcaaggagt ttccagtgcc gaccctcgac
     1021 atcgagatag gcagcactcc ttccagctgg gaagatccgg ctgctgtgga agttcttgct
     1081 aaaagcctgg tccgagcttt ctcgccgttt aaaaggcttt tcaacatgct ctgtgtggga
     1141 gggatccact ttgaacggtc attctcggag gcagccctga acagcgactt ccccttcgga
     1201 gtttcccata tcctccccaa ccagtggatc gtatccggta actacgcctc tgaggagggt
     1261 ttcgaaaagc ttcggtccgc cgctttgagc atcaacggag gcgttgacgc gttagtttac
     1321 catgaaggca tcaagtcccc gttccgcgag cagtgccgca ggttggggga ggagctcgga
     1381 atccctgtgc tcaagcacaa ggccctgagg aagccggaag atctctgttc acccacctaa
     1441 gtcaggttat ttgggggcag gtctacctct tcctccccga ctgctcgtcc ctcatccagg
     1501 actgcaggtc gttggaagtg taggccaggt ggtgggccag ggcgaatacg gacctctcaa
     1561 ggtctccagc cgtcaccgcc tcgaggatat cccggtgctg cctttcagat tcacttctca
     1621 ttgaaccaag gtgggtcctg atggttcggt ccgtcctctg ccggagccgt gccaccatgt
     1681 cgagggtaag gggcctgtcg gccggctcgt agaagagggt gtggaagaga aagttcaggt
     1741 cccgccagcg tatgcagtcc tgttcccgct ccatcttctc caggacctct cgggcatcct
     1801 ggattatttt tcgggttatt cccgggaagg ctattctcat gaggctggtc tccagggccg
     1861 ccaccatctc gtagagctcc cgtgcctcct ccagggagag ctctgtaacg aatgcccccc
     1921 tgtacccccg gaactcaacg agaccttccg tttcgagtag ccttagcgcc tccctcaggg
     1981 gaatggggct aaccccgagt cggtgtgcta tctgtgtctg gtgaaggggt ttgccctctt
     2041 caaggatacc cctgtaaata gcctcccgaa ggacgttggc tatgtggtga ggggcgcttg
     2101 tatcgccggt catcctcccg gccagttcct cgatggtcct gttgttcatc ggcaaaacct
     2161 ccctgccccc cctgtgtagt aacgagaata ttatattatc cagcgccggg attatacgaa
     2221 ccccctggca aactatactt cgttgacacc tctgtggagt gaaaagcata ctggttatgg
     2281 caaataacaa atccgcgaca gggagtggta gccgtggcag ttcccatgaa gtttttgaca
     2341 gaagatctgg aggccatgaa acgggacggt ctttacggaa cgatccgtac catagagagc
     2401 ccccaggggg cgtgggtcaa gatcgccggc aaggattata tcaacctctg ctccaacaac
     2461 tacctggggc tctgcaacga ccccaggctc gtcaaaaagg tcaaggacta tgtagacaag
     2521 tacggaatcg gtccaggtgc cgtcaggacc atcgccggga ccatgatacc ccatatcgaa
     2581 ctcgaaaggc gccttgcgga gttcaaggga gccgatgccg cgatagtcct ccagtcgggt
     2641 ttctgcgcca acctggcagt gatcccgacc ctggtgcctt cagccgatga caccatctac
     2701 agcgactccc tcaaccatgc ctcgatcatc gatgcctgcc gtctctccaa agcgaaggtg
     2761 gtaaggtacg agcactccaa tctggacgac ctgactgcga aactggaaga gttcaagggt
     2821 gcccccggaa agcacctcct ggtcaccgac ggggtcttct ccatggacgg ggacatagcg
     2881 aaactaccgg agatcgttga gatctgcgag aaatacggtg tgatagtagt tgtcgatgac
     2941 gcccacggcg aaggggttct gggccgtggc ggccgaggga tagtggacca cttcggtctt
     3001 cacggcaagg tggatgttga agtgggaacc atgtcaaaag ccttcggagt gatgggcggt
     3061 gtcatagccg ggagcacgac cctgatcgat tatatccgtc agaaggcccg tcccaacctt
     3121 ttcagtagtg ccctcaccgt tcccgatatc gcagccaacc tggcctcggt ggatatcctc
     3181 gaggagagcg aagaacttgt aaccaggctc agggagaacg gcgatttcct gaaaatggaa
     3241 ctctccgcta tcggtttcaa caccggcaac agcgagacgc cgatcactcc ggtcataatc
     3301 ggggaagccc aggacgccaa gaccttcagt gcgaagctat tcgagaacgg ggtctttgcc
     3361 acggccattg tcttccccac ggtgccgaag gggaccgcca ggatccgctg catggtctcg
     3421 gcggcccatt ccagggacga cctcaacctt gcggtggagc gcttcgcaaa ggtcggtagg
     3481 gagatgggga tcatattatg aagaggatcc ttgtcactgg ttcaaacggg cagataggag
     3541 ttgaactggt tccctacctg cgcaagaatt acggtgcgga gaatgttctt gcgacagcga
     3601 gacggaaagt gtcagggccc gtttccgagg gcggtccctt cgagatactc gaggtccggg
     3661 acgggaagac gttctcagag ttgatggatt ccttcaaagc cgacgcgatc attcacctgg
     3721 cggggatact ctccgccagg ggcgaggcat gtccccagga gtcgtgggac atcaacgccg
     3781 gggggtgctt tactgccctg gagtgcgccc gcgaacacgg ggcgtctttc ttcttcccca
     3841 gttcaatagc cgcattcggt ccttcgaccc cggcagacag taccccccag gaaacaatac
     3901 agcgtccgac aactatctac ggtgttgcca aggtgacggg ggaactgctc tgtgactatt
     3961 accacctgaa attcggcctt gataccagag gtctccgctt ccccggcctg atatcctatg
     4021 aggctctgcc cggaggcggc actacggact acgccgttca catctactac gaagccgtct
     4081 cgaaggggaa atacacgagt tatatcgctc ccgggacctg catggacatg atgtacatgc
     4141 ccgacgctct ggagtcggtg gtgcagctca tggaggccga tccctcgaaa ctggtgcacc
     4201 ggaacgccta caatatctcg gccatgagct ttgatcctga tcagatcaca gcgtcgataa
     4261 agaaggtcat tccctcgttc gagatctcct acgacgtaga ccccgtcagg caggggatcg
     4321 ccgaatcgtg gccgaattcc ctggattgct cggcagcgaa agctgagtgg ggttttgcgc
     4381 ccaagtacga ccttgacgcc atgacggtgg atatgctgga gagagtgggg aagaaataaa
     4441 aggcgtcagg cgacaggctg aaggcggcag gaaaaccttt agggcgtaag actagaaagc
     4501 aggacccaag gccaatattg caccacagag ggcacagaga aaagcaagta tatggtttag
     4561 gggcaagagc gaaaaccaaa ggctttggga tcagaccttg acggcctgtt gctcaattga
     4621 gcacccggct tttcaaaagc agatcaggca ggacaggtaa ccggttgttc tatgtaagca
     4681 agacaggatc gcctgagaat ttttgaaaaa agaaagcaga cccttgaaag gcctggttca
     4741 aggatccggg ctatcactgc cggatccttt gcggacctgc cgcccccctt tagcagcacc
     4801 tggatgttct gtacggcaca tgtaaggtat tcctgaatgc tcatcctcca caatcctctc
     4861 cagcgggccc gatcatagcc gtaacgcttg cccctggcga aggagcgttc catgaggtgc
     4921 tgcctct
//
LOCUS       DAOPKQ010000094         4921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18609_length_4921_cov_4.590423, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000094 DAOPKQ010000000
VERSION     DAOPKQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18609_length_4921_cov_4.590423"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PK146_04370"
     CDS             complement(<1..403)
                     /locus_tag="PK146_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91508.1"
                     /translation="MLKGVTSAWLHGLAATSIALLFLAVSPGSPVVDRAVEVTAVVLE
                     LPERPALWARHMVSEGASWVGGVRRLQGRVRSLELENARLRATLEERSELRVTSGSGL
                     VPARIDIRPPALWWNEVRIDKGSRDGIEEGMP"
     gene            complement(459..1514)
                     /locus_tag="PK146_04375"
     CDS             complement(459..1514)
                     /locus_tag="PK146_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPE91509.1"
                     /translation="MFGSISGIFGKDIGIDLGTANIVVYVKDKGVVIDEPSIIAYRKA
                     NRRSAKEIIAYGGEAKAMAGKTPVGVETVRPLQNGVIADFDMTGALIRHFLSEANNGI
                     RPMGHPRVIICAPVEITEVERKAFIDATLDGGAREAYVVEEPVAAAIGVGLPIDEPRG
                     NMIVDVGGGTSEVAVLSLSGVVVSNSLRVAGDEMDNALINMMRQNYTLSIGSATAEEL
                     KNTIGSAMPMNSEKEFEVRGRNLKDGLPRGIRISSTEVREALNPAITQIVEMVRETME
                     RTPPELARDIADQGLVLTGGVALLEGFDRRISQDLNVPVITAERPLFSVADGIGKLLD
                     QFDFMKKVLVSVGHGVR"
     gene            complement(1522..2226)
                     /locus_tag="PK146_04380"
     CDS             complement(1522..2226)
                     /locus_tag="PK146_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HPE91510.1"
                     /translation="MRLAFSSRTELPRERIERLGPQVLSLAEIIAILLRTGDRGKNVL
                     DLASSLVEEFGGLQGLSRTSFREFMEIGGLGRAKAASLVAALELARRLYAEEKAAEEG
                     DPRESFRSRLARWSANLADEPREFIVAVFTDRRGKVIEDDMISWGGLDGAVLDLKFLL
                     RRAVRLDAQGVLLLHNHPDGSLNASVEDRLLTEHVERKLEALGMDFLGHFITAGGGLA
                     EVRGRPTEGGRSCESW"
     gene            complement(2223..2864)
                     /locus_tag="PK146_04385"
     CDS             complement(2223..2864)
                     /locus_tag="PK146_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE91511.1"
                     /translation="MLLERFPLGPLWTNGYLVWDDQGKGFFVDPGGDPREVLVEIDRK
                     SIDLEFILLTHGHADHIGGLGLIRTRARQGVMVHEDDAMMLSRPESNLSAFVGNDIEL
                     APAEKLLRDGDVVRAGRLTVMVIHTPGHTMGSSCFLVGEEGDDPVLLSGDTLFARSVG
                     RTDLPGGDERVLLASLEKLSSLSDETRVLPGHGPETTIGEERKHNPFWPGGSM"
     gene            complement(2864..3313)
                     /gene="dtd"
                     /locus_tag="PK146_04390"
     CDS             complement(2864..3313)
                     /gene="dtd"
                     /locus_tag="PK146_04390"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPE91512.1"
                     /translation="MRAVVQRVARAKVLTDNKVVGSIGKGLCVFLGVAPEDGEREVEW
                     MAEKITNLRIFEDLSGKMNLSLVDEGGAMLVVSQFTLYGDCRKGRRPSFASACEPEKA
                     NRVYEKFIRSVSSRGVPVESGVFQAFMQVEICNDGPATLIIDTSGGC"
     gene            complement(3322..>4921)
                     /locus_tag="PK146_04395"
     CDS             complement(3322..>4921)
                     /locus_tag="PK146_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HPE91513.1"
                     /translation="IDPEAFFDIRRRVKKKLPEREEIIKQAISILKRRFEEEHIAAFI
                     TGRAKHFYSIYEKMRRKNISLEELFDLLALRITVKDVAECYSALGIVHTIWKPLPGQF
                     DDYIANPKSNLYQSLHTAVLGPSGEPLEIQIRTSEMHWIAEYGIAAHWRYKEKRHKID
                     KLDERLSWIRQALESQSEGSEASDFMEHVKADVLSTDVFVFTPKGDVLSLPGGATPID
                     FAYAIHTEIGHKFVGAMVNNRIVPIDYQIQNGDIIKVLTSPQGHPSRDWMKVARSSKA
                     RSKIKSWLRQAEKTEREERLEKGRELLLREVQRRDGGKREEIHLSGSILKKIASDLGY
                     GHGDDLLAAIGGGGQGASSVVSRYLEIESAQALPGQEQEIEEAGEEVATPKRELDTEI
                     VVEGNRGVMVSLANCCKPVPGDDIVGYVTKTRGITIHRIDCPNVTDHPEERKVEVYWG
                     DLRSSRYQARLKLEGADRPGLFAEVAQAITGLESSITQVRAFVAGSDRGRMIIDLQVR
                     DLEHLYRVIAKLNSIRGVIEVLRG"
BASE COUNT          905 a         1645 c         1316 g         1055 t
ORIGIN      
        1 caggcatccc ctcctcgata ccgtcccggg agcccttgtc gatccgtacc tcgttccacc
       61 agagagcagg cggtctgatg tcgatccggg cagggacgag ccccgatccc gatgtgaccc
      121 ttagctcgga cctctcctcc agggtggctc gcagccgggc gttctcgagt tccagggatc
      181 gtacccttcc ctgaagcctt ctcacgccac cgacccagga agcgccttcg gagaccatat
      241 gacgggccca gagggcgggc cgttcaggca gttccaggac gacggccgtc acttccaccg
      301 ccctgtcgac cacaggtgac ccgggagata cggcaagaaa gagcaatgct atcgaagttg
      361 cagccaggcc gtgaagccat gccgaagtca ctcccttaag caatccgaca cccctgttct
      421 ggaaagatcc ctgtccatca ggaacggccg cggaggcgtc accggacccc gtggcccacc
      481 gatacgagga ccttcttcat gaaatcaaac tggtccaaca gtttcccaat tccatctgcc
      541 accgagaaga gtggtctctc ggccgttatt acagggacgt tcaggtcctg ggagatcctc
      601 ctgtcaaatc cctccagaag ggcaacgccg cctgtaagga ccagcccctg gtcggctatg
      661 tccctcgcca gttccggcgg ggtccgctcc attgtctccc tgaccatctc cacgatctga
      721 gtaattgcag ggttcagggc ctctcggacc tcggtgctgg agatcctgat ccctctcggg
      781 agcccgtcct taaggttcct gccccttacc tcgaactcct tctcggagtt catcggcatt
      841 gccgaaccta ttgtgttctt cagctcttcg gcggtcgcgg agccgatcga aagggtgtag
      901 ttctgcctca tcatgttgat aagggcgttg tccatctcgt ctccggccac cctgagggag
      961 ttgctcacaa ccaccccgct cagggagagg accgccactt cactggtacc gccgcccaca
     1021 tcaacgatca tgttcccgcg gggttcgtct atggggagcc ccacgccgat ggccgccgcc
     1081 actggttcct cgacgacgta ggcctcccgg gcgcctccgt caagggtcgc atctatgaag
     1141 gccttgcgct ccacctccgt gatctccacg ggggcacaga tgatgaccct tggatgtccc
     1201 atgggacgga tcccgttgtt ggcctccgag aggaagtgcc tgatcagtgc cccggtcatg
     1261 tcaaaatcag cgatcacgcc gttctggagg ggacgtaccg tctcaacccc gacaggggtc
     1321 ttgccggcca tggccttggc ctcccccccg taggctatta tctccttggc gctccgcctg
     1381 ttggccttgc gataggctat tatcgagggt tcgtcaatga ccacgccctt gtccttgacg
     1441 tagactacga tgttggccgt tccaaggtcg atgccaatgt ccttgccgaa aataccggag
     1501 atcgaaccga acacttctac ctcaccaact ttcgcaagat cggccgcctt ctgtgggccg
     1561 gccccgcact tcagccaacc cgcccccggc ggtaatgaaa tgccccagaa aatccatccc
     1621 cagtgcctcc agtttcctct ccacgtgctc agtcagcagt ctgtcctcga cactggcatt
     1681 aaggcttccg tcaggatgat tatgaagcaa aagcactccc tgtgcatcaa ggcggacggc
     1741 cctcctgagg aggaacttca ggtccaggac ggccccgtcc aggccgcccc aggatatcat
     1801 gtcatcctcg ataaccttgc ctctgcggtc ggtgaagacc gcaacaatga actccctggg
     1861 ttcgtcggca aggttggcgc tccagcgggc aagacgtgaa cggaaagact cccgggggtc
     1921 gccctcttct gcggccttct cctctgcata aagtctcctg gcgagctcca gggccgctac
     1981 aagcgaggcc gccttggccc tccccagccc ccctatctcc atgaactccc ggaaggaggt
     2041 cctggaaagc ccctgaaggc ctccgaactc ctccaccagt gaagaggcca ggtccagaac
     2101 attcttgccc ctgtcgccgg ttctcaggag tatcgctatg atctccgcca gagaaaggac
     2161 ctgcggccct agtcgctcta tcctctccct gggaagctcc gttcgggagg aaaaggccag
     2221 cctcacatgg atccccccgg ccagaagggg ttgtgctttc tctcctcacc tatggtcgtc
     2281 tccggcccgt gcccagggag caccctcgtt tcatcggaga gcgacgacag tttttccagc
     2341 gaggccagaa gcaccctctc gtcaccaccg gggaggtccg tcctgcccac gctccttgcg
     2401 aagagtgtgt ccccggagag gaggaccggg tcatccccct cctccccgac caggaaacag
     2461 gagctgccca tggtatgccc cggtgtgtgg atgaccatca ccgtcagacg gcctgcccgg
     2521 accacgtcac catccctgag cagtttctcc gccggcgcaa gttcgatatc gttaccgacg
     2581 aaggccgaaa ggttcgactc cggccgagaa agcatcatgg catcatcctc atggaccatg
     2641 acaccctgcc gggccctggt acggatgaga cccaggccgc cgatatggtc cgcgtggcca
     2701 tgagtgagga ggatgaactc caggtcgata ctcttcctgt cgatctcgac aagaacttcc
     2761 cggggatcac ctcccggatc gacgaaaaaa ccctttccct ggtcgtccca gaccaggtat
     2821 ccgttggtcc acagaggccc aagcgggaat ctctccagca gcatcagcaa ccccccgaag
     2881 tatcgatgat aagtgttgcg ggcccgtcat tgcagatctc gacctgcatg aaggcctgga
     2941 ataccccgct ctcgacgggg acacccctgg atgatacgga cctgatgaac ttctcgtata
     3001 ccctgtttgc cttttcaggc tcgcaggccg aagcaaatga gggccgcctc cccttgcggc
     3061 agtccccgta gagggtgaac tgggagacca ccagcatggc tccaccctcg tcgaccaggg
     3121 acagattcat cttgcctgag agatcctcga atatcctcag gttcgtgatc ttctccgcca
     3181 tccactccac ttccctctcg ccgtcctccg gggccacgcc caggaagacg caaaggccct
     3241 tcccaatgga accgaccacc ttattatccg tcaggacctt cgccctggcg actctctgaa
     3301 caaccgcgcg catcgggacc ctcaccccct gagaacttcg atgacccccc tgatggaatt
     3361 gagcttcgca atgaccctgt agaggtgctc caggtccctg acctggagat cgattatcat
     3421 ccggcccctg tcgctaccgg ccacgaaagc ccttacctgt gttatggagc tctcaagccc
     3481 ggttatggcc tgggcgactt ccgcgaacag acccggcctg tcggctccct caagtttcag
     3541 tcgtgcctgg taccgggaag aacgcagatc gccccagtat acttcaacct tccgctcctc
     3601 ggggtgatcg gtgacattcg gacagtcgat cctgtggatc gttatacccc tggtcttcgt
     3661 tacatacccg acgatatcgt cgccagggac gggcttgcag cagttggcga gggacaccat
     3721 caccccgcgg ttgccctcaa ccacgatctc cgtgtcgagc tcccgcttgg gtgttgcgac
     3781 ctcctctcct gcctcctcta tctcctgctc ctgcccgggc agggcctggg cgctctcgat
     3841 ctccagatac ctggagacca ccgaagaagc cccctggcct ccccctccaa tggccgcgag
     3901 aaggtcgtcc ccatgcccat accccaggtc gctggcgatc ttcttcagga tagagcccga
     3961 aaggtggatc tcctctctct tgccgccgtc ccggcgctgg acctcacgaa gaagcagctc
     4021 cctgcccttt tcgagcctct cctctctttc tgtcttttcg gcctggcgca accatgactt
     4081 tatcttgctc ctggccttgc tgctccgggc caccttcatc cagtccctgg acgggtgccc
     4141 ctggggggag gtgaggacct tgattatgtc cccgttctgt atctggtagt caatggggac
     4201 tatccggttg ttcaccatgg cccccacgaa cttgtgaccg atctcggtgt ggatcgcata
     4261 agcgaagtct atcggggtag ctccccctgg aagggagaga acgtccccct tgggggtgaa
     4321 gacgaagacg tccgtggaga gaacgtcagc cttgacgtgc tccatgaagt ccgatgcctc
     4381 gctcccttcg gactgggact cgagggcctg cctgatccag gagagccttt cgtcaagctt
     4441 gtcgatcttg tgcctcttct ccttgtacct ccagtgggcc gcgatcccgt actcggctat
     4501 ccagtgcatt tcgctggtcc tgatctgtat ctccagaggc tccccgctgg gacccagcac
     4561 agctgtgtga agggactggt agaggttgct cttggggttt gctatgtagt cgtcgaactg
     4621 ccccgggagg ggtttccata tggtatggac tatgcccagg gcgctgtagc actccgccac
     4681 atccttcacg gtgatcctga gagccaggag gtcgaagagc tcctccaggg atatgttctt
     4741 tcttcgcatc ttctcgtaga tgctgtagaa gtgctttgcc ctgccggtaa tgaaggcggc
     4801 gatgtgctcc tcttcgaaac gtctcttcag gatgctgatg gcctgcttta tgatctcctc
     4861 tctctcgggg agcttcttct tgacccttct tcttatatca aagaaggcct ccgggtcgat
     4921 g
//
LOCUS       DAOPKQ010000095         4912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18656_length_4912_cov_3.741404, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000095 DAOPKQ010000000
VERSION     DAOPKQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18656_length_4912_cov_3.741404"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PK146_04400"
     CDS             complement(<1..925)
                     /locus_tag="PK146_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE91514.1"
                     /translation="MRKIFVLALLAVFAVTGFAAGAMADDVIKVGYLAALTGDWAAYG
                     QTELNAAKLAVAEINAKGGVLGKQIQLFPYDFRTRPEDAVNAFRRMAENDKVVAVVGA
                     NGSGINIATAPLANRYMIPQIETVSTNLLVTVSDQGELNPYMFRTCFTDPYQGQLIAA
                     FTAKELKKNTAAVLYDVGSDYAQGLREFFMKYYEDYGGKVVADQGFRAGTDVDFRAQL
                     TTIRDSKAEVLVLPNMGKEMALIMKQARELGMNDIIFVGGDGYGEFMWEIAGDAMENS
                     YWINHVAPEDPAMADFFANYKKTYNDECKEFV"
     gene            1074..1982
                     /locus_tag="PK146_04405"
     CDS             1074..1982
                     /locus_tag="PK146_04405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPE91515.1"
                     /translation="MRKLLVTDIDGTLAHRDNIPEEVVQTCLGLRQEGWEIVVATGRI
                     LASARGHMKSVGALPQAIVYDGARIMSNETGKEIWGATINPETVEGILRTIWDLAPGI
                     QVFGDEKVFCRPDDTLARKYFSSLGVPVIDDLACPRAIRGVFRVIIHGQAGSVEEIGE
                     DVSAAIGDSARAVFAGDGFLDILGPGISKGAALDRLLGSLEEEGSPVLVAAAGDHLND
                     MELLEYAHISITMEDAREPLLAMADVILPPASEKGFSRISGPLRSLAGPFEALSYEGR
                     LPRGRVCKTSPGERPQGGMFNGGEGH"
     gene            1963..2463
                     /locus_tag="PK146_04410"
     CDS             1963..2463
                     /locus_tag="PK146_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPE91516.1"
                     /translation="MAEKGIKWEVVDIGIPEGCNVILGQSHFIKTVEDLYEVLATSSP
                     ALEFGLAFCEASGPCLVRKDGNADDLVEAAVRNAMKLSAGHAFVILMRNGFPVSVLNS
                     VKQVQEVCRIFAATANPLQVLVFQTGQGRGIAGVVDGFPSKGIESGDDIEERKRLLRD
                     IIGYKR"
     gene            complement(2466..3854)
                     /locus_tag="PK146_04415"
     CDS             complement(2466..3854)
                     /locus_tag="PK146_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPE91517.1"
                     /translation="MSIFALSALSVMLAFMVAGVWSGRRSRSHSEYTVAGRASGPVQV
                     SGILLGSLVGGASTVGTVQMAYQWGLSAWWFTLGGGLGCLVMGLWFAAPLRRSGVETI
                     PGFLNISFGKAVSAVSVVASITGTFISIVVQFISGSALLRGVFPAGGTISVLLVGVLV
                     LSFIFTGGIKTFSSLGVWKIVVLYLALVLCAGAAIRSFLLLPEGTVPLPFNPFFNLFG
                     QGLGKGLGAGLSVVTGVMCTQIYVQAVFSASEPATARKGALLSAFLMPPMGLMGIVVG
                     LGLRRSGVILESGTALSHFLATSFSPLAGGILWGVILVTLIGTAAGLTLGVATNLVCD
                     LLPSFRLYKYCESNLTGTTRITVLVLVVLAGLTGLAGQNSMILKWSYLSMGLRASGTF
                     LPLVAAVLAPGSLSPRWALATGSTGLLTTLLWPLTGLPQEPLTAGLLASAMVAGAGIL
                     SGRGPLSRRGGI"
     gene            4038..>4912
                     /locus_tag="PK146_04420"
     CDS             4038..>4912
                     /locus_tag="PK146_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HPE91518.1"
                     /translation="MAKKVQVFKCSECGYQSPTWSGRCPSCQEWGSLVEMISPSGPVK
                     PGLKAKPSFIPDIALPRRFASGIGEFDRVLGGGFVPGTVTLLGGEPGVGKSTLLLQAC
                     AAMAAGGQRVLYVSGEESLSQVAIRAKRIGNGNGGDLGIISATSIDESLDSVEGSSIA
                     VFDSIQSFTVEEEGGFPGTPTQVRAVAQKIIERSKATAVPSVIIGHITKQGSIAGPKL
                     LEHMVDVVLMFAGDRSSPYRLLRSYKNRFGSTDELGVFEMSDKGLVPVQDPSRLFWST
                     AGEPVSGVAMGVIMEG"
BASE COUNT         1027 a         1450 c         1555 g          880 t
ORIGIN      
        1 tgacgaactc cttgcactcg tcgttgtagg tcttcttgta gttggcgaag aagtctgcca
       61 ttgccgggtc ctcgggggcc acgtggttga tccagtagct gttctccatg gcatccccgg
      121 cgatctccca catgaactcg ccgtagccgt ctccgcccac gaagatgatg tcattcatcc
      181 cgagctcgcg ggcctgcttc attatcaggg ccatctcctt gcccatgttg ggaagtacga
      241 ggacctcggc cttgctgtca cggatcgtgg tcagctgtgc cctgaagtcg acatcggtgc
      301 cggcccggaa gccctggtcg gcaacgacct ttccgccgta atcctcgtaa tacttcatga
      361 agaactcgcg aagcccctgg gcgtagtcgc tgcctacgtc atagaggacg gcggcggtat
      421 tcttcttgag ctccttggcg gtgaaggccg cgatgagctg tccctggtag gggtcggtga
      481 agcatgtgcg gaacatgtag gggttcagct caccctggtc gctgacggtt acaagaaggt
      541 tggtggacac ggtctcgatc tgggggatca tgtacctgtt ggcaaggggc gcggtggcta
      601 tgttgatgcc gctcccgttg gctccgacca cagctacgac cttgtcgttc tcggccattc
      661 ttctgaaagc gttaactgca tcttcaggac gggtcctgaa gtcatagggg aagagctgga
      721 tctgcttgcc aaggacccca cccttggcat tgatctcggc gaccgccagt tttgccgcat
      781 tgagctctgt ctggccgtag gccgcccagt cacccgtgag ggctgccagg tatccgacct
      841 tgatcacgtc gtcggccatg gcgcctgctg cgaaaccggt taccgcaaaa acagcaagca
      901 aagcaagcac aaagatcttc ctcaatacgt acacctcctg agaaggttat ggaaatattt
      961 acatggtacc ataattataa tgcatgactg cgggtgtcaa gtttattttt ccccgtttaa
     1021 cctgtaatat cgccttgttc cccgagcaag gcttccgggg acagggggat atgatgagaa
     1081 aactgctggt gaccgatatc gacggaaccc tggcccaccg cgacaatatt cccgaagaag
     1141 ttgtccagac ctgcctgggc ctcaggcagg aaggatggga gatcgtcgtg gccacgggcc
     1201 gcatcctggc cagcgcccgg gggcatatga agagcgtggg cgccctcccc caggccattg
     1261 tctacgacgg cgcaaggatc atgtccaatg agaccggtaa ggagatctgg ggcgccacca
     1321 taaatcccga gacggtggag gggatactgc ggaccatatg ggacctggcc ccggggatac
     1381 aggtattcgg cgacgagaag gtcttctgca gacccgacga tactctggca aggaagtatt
     1441 tcagcagcct cggcgtccct gtgatcgacg acctcgcctg ccccagggcg atacggggcg
     1501 tcttccgtgt gatcatccac ggccaggccg gcagcgtaga agagataggg gaggatgtct
     1561 ctgccgccat aggcgacagc gcccgggccg tctttgccgg agatggcttc ctggacatac
     1621 ttggtccggg catctcgaag ggggctgccc tggacaggct tctggggtcc ctggaggagg
     1681 agggcagccc cgttctggtg gcggccgccg gggaccacct caacgacatg gagctgcttg
     1741 agtacgctca tatctcgata accatggagg acgccaggga accgctcctg gccatggccg
     1801 acgtgatcct ccccccggct tccgagaagg gcttttcaag gatatccgga cctctcaggt
     1861 ctctggcggg tcctttcgaa gcactatcat acgaggggcg cctccccagg gggagggttt
     1921 gcaagacctc cccgggagag cggccccagg gaggtatgtt caatggcgga gaagggcatt
     1981 aaatgggaag tggtcgacat agggataccc gagggctgca acgtgatcct gggccagagt
     2041 cacttcatca agaccgtgga ggacctctac gaggtgctgg ccacttcgtc gcccgcgctt
     2101 gagttcgggc tggcattctg cgaggcttcg gggccctgcc tggtccgaaa ggacggcaac
     2161 gccgacgacc tcgttgaggc cgctgtgcgt aacgcaatga agctgtcggc ggggcacgcc
     2221 ttcgttatct taatgaggaa cggcttcccc gtcagtgtcc tcaactcggt gaagcaggtc
     2281 caggaggtat gccgtatctt cgctgccacc gcgaaccccc tgcaggtcct tgtcttccag
     2341 acgggtcagg gtagggggat cgccggcgtc gtagacggct tcccctcgaa aggcatcgaa
     2401 tccggtgatg atatcgagga gagaaagagg cttctgaggg atattatcgg ctacaagagg
     2461 tagcttcaga tcccgccccg acgggagagg ggcccccggc ccgacagaat gcccgctccc
     2521 gcaaccatgg ctgatgccag gagccccgcc gtaaggggtt cctggggcag acctgtcagg
     2581 ggccagagaa gggtcgtcag caggcccgtc gagcccgtgg ccagggccca gcggggagac
     2641 aggctccccg gggccagaac ggcggcgaca agggggagga atgtccccga ggccctgagc
     2701 cccatgctca ggtagctcca cttgagtatc atcgagttct gccccgccag accggtcaaa
     2761 cctgcaagga cgaccaggac aaggaccgtt atcctggtcg tcccggtcag gttactctcg
     2821 cagtacttgt agagcctgaa cgacggcagc agatcgcaga ccagattggt ggccaccccc
     2881 agcgtaaggc ccgcagcggt ccctatgaga gtgaccagga tcacccccca gaggatcccc
     2941 ccggcaaggg ggctgaagct tgtggccaga aagtgcgaaa gggccgtccc cgactccagg
     3001 atcacccccg atctcctcag gcccagcccg acgactatgc ccatgagccc cattggaggc
     3061 attagaaagg ccgaaagaag ggcgcccttt ctcgcggtgg ccggttccga ggcggagaag
     3121 acggcctgga cgtagatctg ggtgcacatt acacccgtta ccaccgaaag tcctgccccc
     3181 aagcccttgc ccagaccctg cccgaagagg ttgaagaagg ggttgaaggg caggggtacg
     3241 gtcccctcag gaagaaggag aaaggacctt atcgcggccc ccgcgcagag tacgagcgcc
     3301 aggtagagca cgacgatctt ccagaccccc agggagctga aggtcttgat acctccggtg
     3361 aatatgaagg acaggacaag gacccccacc aggagcaccg aaatggtgcc ccccgcaggg
     3421 aagacccccc tcaggagagc cgaaccggag atgaactgga cgacaatgga gatgaaagtg
     3481 cccgttatcg acgcgacgac cgatacagcc gatacggctt tcccgaaaga tatgttcaga
     3541 aagcccggga tggtctccac tcctgaacgc ctcagggggg cggcgaacca tagccccatg
     3601 acgaggcagc ccagccctcc ccccagggtg aaccaccatg ctgaaagtcc ccactggtag
     3661 gccatctgta cggtacccac cgtagaggct cctccgacaa gagaacccag gagtatcccc
     3721 gagacctgaa cgggtccact cgctcttccg gcaacggtgt actccgagtg tgaccgcgac
     3781 ctccggccgg accagacgcc ggcaaccatg aaggcaagca ttacagagag ggctgaaagg
     3841 gcgaagatcg acatttccgg ctgacatcct tccgtacaaa gaaattggcc cagcccatga
     3901 tacacgaaag gcaaccctgc gtggaccggt ccgccgcccc tcggaggcaa ggggagagga
     3961 tgcaaaaaaa ggaccgatgc gctatgataa acaaagaaaa ggcatgaagg cccgttcggg
     4021 cctggatcgg ggtggacatg gcaaaaaagg tccaggtatt caagtgcagc gaatgcggct
     4081 accagagccc tacctggtcg gggaggtgcc cgtcctgcca ggagtggggc tcccttgtgg
     4141 agatgatctc tccctcgggg ccggtcaagc cggggttgaa ggccaagccg tctttcatac
     4201 ccgacatagc ccttccccgg aggttcgcca gcgggatcgg ggagttcgac agggtattgg
     4261 ggggaggctt cgtacccggg acggtgaccc tcctcggcgg tgagccgggc gtcgggaaat
     4321 cgacgttgct tttgcaggcc tgcgccgcca tggccgctgg tggccagaga gtcctgtacg
     4381 tttcgggcga ggagtccctc tcccaggtgg ccatccgggc gaagaggatc ggcaatggca
     4441 acgggggcga cctgggaata atcagcgcca cctccatcga cgaatccctc gactccgtcg
     4501 agggaagctc catagccgtc ttcgacagca tccagtcctt caccgtcgaa gaggagggag
     4561 gattcccggg gacgcccacc caggtaaggg ccgtcgcgca gaagatcatt gaaaggtcaa
     4621 aggccacggc agtgccgtcg gtcatcatag ggcatatcac caagcagggt tccatcgcgg
     4681 ggcccaaact gctggagcac atggtggacg tggtcctgat gttcgccggg gacaggtcgt
     4741 cgccctacag gcttctgagg tcatacaaga accgtttcgg gagcaccgac gagctcgggg
     4801 tcttcgagat gagcgacaag gggcttgtac cggtacagga tcccagcagg ctattctgga
     4861 gcaccgccgg cgagcccgtc tcgggggtgg ccatgggggt catcatggag gg
//
LOCUS       DAOPKQ010000096         4897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18734_length_4897_cov_5.856051, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000096 DAOPKQ010000000
VERSION     DAOPKQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18734_length_4897_cov_5.856051"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /gene="flgI"
                     /locus_tag="PK146_04425"
     CDS             complement(<1..1750)
                     /gene="flgI"
                     /locus_tag="PK146_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HPE91519.1"
                     /translation="MKQLKKIFIAMLISLSALLAAGILPAGGQVHPEVRLKDLGRFEG
                     VRPNQLVGMGLVTGLQGTGDKGELVAAMMGNMVENFGISMNRKDLKSKNSAVVTITCE
                     LPPFARSGETIDVTISTVGDAKSLEGGVLLQTPLKAANGSVYAVAQGPVSIGGFSASA
                     AGSSLSKNVSTTGRIPNGAIVERETSWGAGTDGRIRFFLKRPDFTTADRVAAIINRNY
                     GSVATVTDAGCVDIAIPHAFRGNPSGFIASSELLRVRPDSVARVVINERTGTVTVGGD
                     VRISEVAVAHGNLTVSVGIDRTVSQPEPLSLGETKVIETATITADEERGSMALLNATT
                     TVDDVVATPDAVWLLSQRTIYRLPREPQQEAMHRPAPRFTSCRVDGRPVTMGEVTFLN
                     ADQRDVELHFRVALPERFGQEQYRVRMGSGRFSQIGAGRLDLFNLGPGRQRIEVEAGM
                     PGGGWGPVGSLVLDVAPRLHERLWARLLAGLIVVLLVAAFLRWREFRLRERLATQRDL
                     DRLQMQALTSQLEPHFVFNALNGIQSYLARNDRDAGMRYLARFSRLMRGLLETGQSRV
                     IPLAHEVELLEHYCTLE"
     gene            complement(1760..2335)
                     /locus_tag="PK146_04430"
     CDS             complement(1760..2335)
                     /locus_tag="PK146_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HPE91520.1"
                     /translation="MRIVKILFLMVAATLVFSAPAIAESLWAEGSSLFQDHRPDKVGD
                     IVTVVVSEKTTAKDSAKTDLTKKNDTTLADGFGLLDFIKKLGFSSSSEMSGGGTRDRS
                     HSFNATITCIVTEVLPNGNIVLEGTKEIRVQKENMSMKITGIARPQDVKAGNILESDR
                     LADVSVNVRGIGSISDVQQPGFLTRLLNIIF"
     gene            complement(2357..3055)
                     /gene="flgA"
                     /locus_tag="PK146_04435"
     CDS             complement(2357..3055)
                     /gene="flgA"
                     /locus_tag="PK146_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HPE91521.1"
                     /translation="MKKSLFFAALLALLAFSAPAISMEPVSLEGLQVRWGEGTETLSG
                     MLRQRGGFPWSGITMEPGSFPFSGEIVSPKRIYPGTSSAVFRIRTWGGGTESFVARLT
                     WHAPMVLALEEISRGSVITGSNAAVQVAPYRRGYGKVFCSLPQVEGKRASRDIRAGEP
                     LSGRNIEVILLVERRDPVVVISKSGAVIARLRGVALEGGDLGDVITVRVPRYRNEIPA
                     TVIDRGLVEAAAGL"
     gene            3189..>4897
                     /gene="flgK"
                     /locus_tag="PK146_04440"
     CDS             3189..>4897
                     /gene="flgK"
                     /locus_tag="PK146_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPE91522.1"
                     /translation="MSGTFSGLEIGRRALDYFRQGIETAGHNISNADVEGYSRQRVEA
                     APADPYAAVGFNSPGSPGQVGTGVEVSAISRIRDSFLDAQFRQESIEGGYWEALSRTM
                     EYLEMFVCEPSGTGLSASLDNFVQALHEFQKRPDSGPAREVLLREAENLSSLMGQISG
                     NFSEYRDSLNTEIELRVGEANSLIDRIAALSGQISRVQAMGNSPNDLMDRRDLLVEQL
                     ARLVDVSVYYSSVSNEYLVDVGGKNLVQGSEARHLVLVPQEGNGGLFDVQVENNLFST
                     ADKPEVAVYTVLRKAAEGIHTMEVEHSATETRWAAGAVSGVGCFASATESLGLDGGFT
                     LQVGTDGMVASSGSVPGGVVLDAPGPGDALIHLLRIGAGGAEKTIEMTWDGVSSVWML
                     DGTVDAGEELTLSELASYINSAWSGPVTARVDGGRIFLESPDGYLVSVTDARGRVSDA
                     LGMTRSSPAVSIEVNSTDSLQTIANRINSAYGSEGGPARPEEWLHATVEQTHGGLWYL
                     KLESNLVGEAYRINVGPEDGISLYTARNLGLVDPQGATRYSAIARDALFTVDGMKYLS
                     STNA"
BASE COUNT          949 a         1547 c         1453 g          948 t
ORIGIN      
        1 cctccaaggt gcaataatgt tccagcagct ccacctcgtg tgcaaggggg atcacacgcg
       61 actgtccggt ctccagcagc ccgcgcatca ggcggctgaa gcgggccagg tagcgcatac
      121 cggcatcgcg gtcgttgcgg gccaggtagc tctggatgcc gttgagggcg ttgaatacga
      181 aatgcggttc caactgggag gtgagcgcct gcatctgcag acggtccagg tcgcgctggg
      241 tggccaggcg ttcacgcaac cggaactcgc gccagcgcag gaaggcggcc accagcagta
      301 cgacgatgag gccggccagc agcctggccc agagccgttc atgcaggcgc ggggccacgt
      361 ccagcaccaa ggatccgacc gggccccaac cgcccccggg catgcccgcc tccacctcga
      421 tgcgctgccg ccccggcccc aggttgaaga ggtccagccg gcccgccccg atctgcgaga
      481 aacggccgct gcccatgcgc acccggtact gttcctgccc gaaccgctcg ggcagggcca
      541 cccggaagtg cagttccaca tcgcgttggt cggcgttcag gaaggtcacc tcccccatgg
      601 tcacaggccg cccatcgacc ctgcaactgg tgaagcgcgg cgcagggcgg tgcatggcct
      661 cctgctgggg ttcgcgcggc aggcggtaga tggtgcgctg ggacaacaac cacaccgcgt
      721 ccggtgtggc caccacgtcg tccacggtgg tggtggcatt aaggagggcc atgcttcccc
      781 tctcctcatc ggcggttatc gtcgcggtct ctatgacctt tgtctccccc agggaaagag
      841 gttccggctg actgacggtc ctgtcgatcc cgacggaaac cgtcaggttc ccgtgggcta
      901 cggccacctc gctgatcctc acatctcctc ccacggttac cgtccccgtt ctctcgttga
      961 ttaccaccct ggcgacggaa tcgggcctga cccgaagaag ttccgaagag gcgatgaagc
     1021 ctgaggggtt tccccgaaaa gcgtggggga tggcaatatc cacgcatccc gcatcggtta
     1081 ctgtggctac ggaaccgtaa ttgcggttga tgatcgccgc caccctgtcg gcggttgtga
     1141 aatcgggcct cttcaggaaa aagcgtatcc tgccgtccgt accggccccc caggaggttt
     1201 ccctctcgac tatggccccg ttggggatcc gccccgtggt ggagacgttt ttcgaaaggc
     1261 tggagcctgc tgccgatgcg gagaaccctc ctatcgaaac gggtccctgg gctacagcgt
     1321 agaccgatcc gttggccgcc ttcagcggag tctggagcag gacgccaccc tcaaggctct
     1381 tggcgtcacc cacggtgctg atcgtcacgt cgatggtctc accgctcctg gcgaaggggg
     1441 ggagttcgca ggtgatcgtt acgacggcgc tgttcttgct cttgagatcc ttccggttca
     1501 tggatattcc gaagttttcc accatattgc ccatcattgc cgccacaagc tcacccttgt
     1561 cgcccgttcc ctggagtcct gtaaccaggc ccataccaac aagctggttc ggccggacac
     1621 cctcgaacct tccaagatcc ttcagcctga cctcgggatg cacctggcct ccggctggaa
     1681 ggatcccggc cgcaagaagg gccgataacg aaatcagcat cgcgataaag atctttttca
     1741 actgcttcat gattttcacc taaaagataa tgttcaggag ccgtgtgagg aagcctggct
     1801 gctgtacatc gcttatgctt cctatgcccc tgacgttgac gctcacatcg gcaagcctgt
     1861 cgctttcaag gatattgccg gccttcacgt cctggggcct cgcgatcccc gttatcttca
     1921 ttgacatatt ctccttttga acccgtatct cctttgtccc ctccagaact atgttcccgt
     1981 tcggaagcac ctctgttacg atgcaggtga tggtcgcgtt gaaactgtgg ctcctgtccc
     2041 tggtgccgcc tccgctcatc tcgcttgagg aggaaaagcc cagtttcttg atgaaatcca
     2101 gcaaaccgaa accgtcggca agggtcgtgt cgttcttttt cgtcaggtcc gtctttgcgg
     2161 aatcctttgc ggtggtcttc tccgacacga ccaccgtgac gatatcaccc accttgtcgg
     2221 gcctgtggtc ctggaaaagc gaagagcctt cggcccagag cgactccgcg atggcgggcg
     2281 ccgaaaaaac aagagtggca gcaaccatca gaaacaggat ctttacgatc ctcatgtcta
     2341 acacccccat acgccgctaa agaccagcgg cggcctcaac caggcccctg tcgatgacag
     2401 tggccggaat ctcgttcctg tacctgggga ccctgacggt tatgacatca cccaggtcgc
     2461 ccccctcgag ggccactccc ctgagcctgg cgatgacggc cccgcttttg gagatgacca
     2521 ccaccgggtc ccgccgttcc acaaggagga tcacctcgat gttccttccg gaaaggggtt
     2581 ccccggcccg aatgtcccgg gatgccctct tgccttcaac ctgaggcagg ctgcagaaaa
     2641 ctttcccgta acctctcctg tacggggcca cctggacggc cgcgttcgag cccgtgatga
     2701 cgctgccccg ggagatctct tccagtgcca ggaccatggg ggcatgccag gtgagccttg
     2761 ccacgaaact ctccgttccg cctccccacg tacggatcct gaatactgcg gaagaggtgc
     2821 ccggatatat tcttttggga ctgacgatct ctccggagaa ggggaaactc cccggttcca
     2881 tggttatgcc gctccatgga aagccacctc tttgcctgag catcccggaa agggtctcgg
     2941 tcccttcacc ccagcggacc tggagcccct ccagcgagac gggctccatg gatattgccg
     3001 gggcggagaa ggcaagcagc gcgagaagcg ccgcaaaaaa caggcttttc ttcaaattgc
     3061 cattccccct tcctggctgc cgatctgcac aaaacctctc acattgagta tcggacgtgg
     3121 gtgcgaaaag atgaaatctg ttctatgccc ctttccaggc cgataaccaa aaagaggggg
     3181 gatcattcat gagcggtacc ttttcggggc tcgagatcgg caggagggcg ctggattact
     3241 tccggcaggg gatagaaacg gccggtcaca acataagcaa cgccgatgtc gagggatact
     3301 cccgccagag ggtggaggcg gctccggccg acccctacgc ggcagtgggc ttcaattccc
     3361 ccggctcccc cgggcaggtt ggcaccggtg tcgaggtcag cgcaatatcc cggataaggg
     3421 actcgttcct cgatgcccag ttccggcagg agtcgataga gggcggttac tgggaggccc
     3481 tgtccaggac aatggaatac ctcgagatgt tcgtctgcga gccctccggg acgggacttt
     3541 ccgcctcgct ggacaacttc gtccaggccc tccacgagtt ccagaaaagg cccgattccg
     3601 gccccgcccg cgaggttctc ctgagggagg ccgaaaatct ctccagcctg atggggcaga
     3661 tcagcggcaa cttctccgag taccgggact ccctgaacac cgagatagaa ttgagggtag
     3721 gggaggccaa ctccctcatc gacaggatcg cggccctgag cggacagata tccagggtac
     3781 aggctatggg gaacagcccc aacgacctca tggacaggag agacctcctc gtggagcaac
     3841 tggcccgtct cgtggacgta tcggtctact acagctccgt cagcaacgaa tatctcgtcg
     3901 acgtgggggg taagaacctt gtccagggaa gcgaggccag gcacctggtc ctcgtccccc
     3961 aggaggggaa cggcggtttg ttcgacgtcc aggtggagaa caacctcttc tccactgccg
     4021 acaaaccgga ggttgccgtt tataccgttc tcagaaaggc cgcggagggg atccacacca
     4081 tggaggtgga acactcggcc acagagaccc ggtgggcagc gggagccgtt tccggggtgg
     4141 ggtgctttgc ctccgccacg gagagcctcg gccttgacgg tgggttcacc cttcaggtcg
     4201 gtaccgacgg gatggtggcc tcttccggca gtgtccccgg aggggttgtt ctggacgccc
     4261 cgggccctgg cgatgctctg attcatcttc tgaggatagg tgccgggggt gcggaaaaaa
     4321 ccatagagat gacctgggac ggcgtctcgt cggtctggat gctggacggt accgtcgacg
     4381 cgggggagga actgaccctt tccgaactcg caagctacat caacagcgcc tggagcggtc
     4441 ctgtcaccgc cagggtcgat ggtgggagaa tattccttga gagccccgac ggctacctgg
     4501 tatccgttac cgatgccagg gggagagtct ccgacgcgct ggggatgacc agatcgtccc
     4561 cagccgtgtc catagaggtt aacagtaccg actcgcttca gaccatcgcc aacaggataa
     4621 acagcgccta cggttccgaa ggtggcccgg ccagaccgga ggaatggctg catgccacgg
     4681 tggaacagac ccatggcggt ctctggtacc tgaaactcga gagcaacctg gtcggcgagg
     4741 cctacaggat aaacgtgggc cctgaggacg gcatcagcct ctacacggcc cgcaacctcg
     4801 gcctcgtaga tccccaaggg gcaacgcggt actcagccat tgccagggat gcccttttca
     4861 ccgttgacgg catgaagtat ctttcctcga ccaatgc
//
LOCUS       DAOPKQ010000097         4895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18753_length_4895_cov_3.460537, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000097 DAOPKQ010000000
VERSION     DAOPKQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18753_length_4895_cov_3.460537"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1417)
                     /gene="secD"
                     /locus_tag="PK146_04445"
     CDS             complement(5..1417)
                     /gene="secD"
                     /locus_tag="PK146_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPE91523.1"
                     /translation="MIKKDRWRLILVVIVVAAALLSVFPIDGRIKLGLDLKGGAHILL
                     QAKGTPENPLTEDSVERLLAVLRNRIDQYGVSEPLIQREGSDRVIIDLPGVADPEAAL
                     ELIGKTALLEFRHVFESTGSAPPGPQRENYESQAEFLAAQARWEQAVKQLDELAEEMK
                     IRADEDPGVVMARGQDGEVYLLGKTYVGGRDLEDARTSYDNLGRPVVTLKFTSEGARL
                     FDEATAANVGRQIAITLDGKVISSPVVQERISAGSAQISGRFSAAEAQRLSIMLRAGA
                     LPVSVEILENRSVGPTLGTDSIRSGIRAGLVGGLLVLLFVLIYYRTLGIAANLALCVC
                     MLLVFAGLISLKATLTLPGIAGIILTLGMAVDGNILIFERMKEEVASGKTPYAAVDAG
                     FRKALSAILDANITTLIAAAVLFYFGSGPIRGFAVTLSIGIISSVFSAIVVTRTLLHV
                     FQSGSRGRIKIATSGKGGKS"
     gene            complement(1578..1871)
                     /gene="yajC"
                     /locus_tag="PK146_04450"
     CDS             complement(1578..1871)
                     /gene="yajC"
                     /locus_tag="PK146_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPE91524.1"
                     /translation="MGMLLPLGVFVVIFYFLIIRPQKKRQRKQEEMIASIRRGDQVVT
                     AGGFFGIVREVKEDSFILEIADGVKARVLKASVQMKKFEGEAETPAETGKAGA"
     gene            complement(1998..2624)
                     /locus_tag="PK146_04455"
     CDS             complement(1998..2624)
                     /locus_tag="PK146_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPE91525.1"
                     /translation="MRIAEPTVERLVLYYRLLMQLHNEERRVVSSQEIGDMLGLKASQ
                     VRKDLSFFGEIGKRGVGYHVERLYQHISDILAPKRLWKIALVGVGRLGEALLGHKALR
                     SEKFRIDALFDNDPAKIGTDICGVSCYDSADMAEVIRQKGIEVLVLTVPPAAAQSSVD
                     KGISAGTIRGILTFATSAISVPENVLVCRVDISVELEKMLFFLKGNSD"
     gene            complement(2661..2933)
                     /locus_tag="PK146_04460"
     CDS             complement(2661..2933)
                     /locus_tag="PK146_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPE91526.1"
                     /translation="MRIDRFLKMTHIVKRRSTAREMISLGAVRVGEMAVRPSRLLKPG
                     DTVEIAFPRKILTIKVVSVEEAVVRRRTEAFELVGERNVTGDERPW"
     gene            complement(3026..4141)
                     /gene="rpoD"
                     /locus_tag="PK146_04465"
     CDS             complement(3026..4141)
                     /gene="rpoD"
                     /locus_tag="PK146_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPE91527.1"
                     /translation="MTVAAESGSKEVLRYIESFKELLHMGREKGFVTYDDIEKHLPGE
                     MMNEETLDSLYFNLQELGIDLVETDKPKQDAHDADAPPAAEYHEEMGVLEDLPLSDPV
                     RMYLREIGKISLLKPEEEVELAKLVEAGDPVGKSKLVEANLRLVVSIAKKYIGRGMLF
                     LDLIQEGNLGLIRAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVET
                     INKLIRVSRQLVQRLGREPTAEEIAAELSIAANRVEEIQRIAQEPVSLETPIGEEEDS
                     QLSDFLEDKNLPSPDDAAAVQLLREQLEGMLEELTEREKEVLRLRFGLEDGHPYTLEE
                     VGRRFGVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(4183..>4895)
                     /locus_tag="PK146_04470"
     CDS             complement(4183..>4895)
                     /locus_tag="PK146_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91528.1"
                     /translation="SLLQRAEPLPLFHIRVRRSELEDPAKRTAARREIIRGLAEMPAL
                     EANRHITQVAQGLGILLPEMLDLLRQAREDIPGSKKGIPAAESVYKVGTDPSGEDNAV
                     DPWESALCYLLWVDSKRRSSESVESVLGLLSDDRAKTIAVSLLAGESPGELRTRWAET
                     GDPFPMRMISSGWAHCEKNGEEGEIWEVVISALERKRLRDRFSVLKERHARSEASPQE
                     MKEYLALARRLKGGVGSP"
BASE COUNT          986 a         1525 c         1205 g         1179 t
ORIGIN      
        1 aacatcagct tttcccccct ttgccggacg tggcaatctt tatcctgccg cgactgcccg
       61 actggaagac atgaagaagg gtcctcgtca ccactatcgc gctgaatacg ctcgaaatta
      121 tcccgatcga aagggttacg gcaaatcccc tgatggggcc actcccaaag tagaagagga
      181 ccgccgccgc tataagggta gtgatgttgg catcaaggat agcactgagg gccttgcgga
      241 aacccgcatc gaccgccgca tagggggttt tgcccgacgc gacttcctct ttcatcctct
      301 cgaatatcag gatgttgccg tccacggcca ttcccagtgt aagtatgata cccgcgatac
      361 cgggaagggt cagtgtggcc ttgaggctta tcagtccggc gaatacaaga agcatgcaga
      421 cgcaaagcgc caggtttgcc gctatcccca gggtcctgta atagatcagg acaaataaca
      481 ggaccagaag gcctcccaca agaccggccc ttatacccga cctgatcgag tcagtgccga
      541 gggtaggtcc tacggagcgg ttctccagga tctccaccga tactggcagg gctcccgccc
      601 tgagcattat tgagagtctc tgtgcctccg ccgcggagaa acgccccgat atctgggcac
      661 tgccggcgct gatacgttcc tggaccaccg gggaggatat gacctttccg tcaagggtaa
      721 tggctatctg ccgtcccaca ttcgcagcgg tggcctcgtc aaaaagacgt gccccctcgc
      781 tggtgaactt cagggttaca acagggcgcc cgaggttgtc ataactggtc ctggcatcct
      841 caagatccct tccaccgacg taggttttcc cgagcaggta gacctctcca tcctgacccc
      901 gcgccattac caccccgggg tcctcatcgg ccctgatctt catctcctcg gccagttcgt
      961 cgagctgttt cactgcctgc tcccatctcg cctgggctgc gaggaattcc gcctgggatt
     1021 cgtaattctc cctctgcggt cccggtggag ccgaaccggt ggactcaaag acatgtcgga
     1081 actccagaag ggccgtcttt ccgataagct ccagagcggc ttcagggtcg gcaacaccgg
     1141 gcaggtctat gatgacccgg tcgcttccct ccctctgaat gaggggttcg gaaacaccat
     1201 actggtctat cctgttcctc agaacggcca gaagcctttc cacgctgtcc tcggtcaggg
     1261 gattctcggg agtccccttg gcctggagga gaatatgggc gccccccttg agatcgagac
     1321 caagcttgat acggccatcg atcgggaaga cggataacaa agcggccgcg accactatca
     1381 ccaccagtat cagccgccat cgatccttct tgatcatttt acttcctccc ctactggaac
     1441 cttctcgcgc cgggcaaaca cgacgccgat agtgatttca acaaaaccat ctcataaaaa
     1501 acggagcgga tgcacggcat ccactccgaa ggaagccttg cgctgacgcg ccgcctgcgg
     1561 gacctcctgt cggatcctca ggctcctgcc ttgccggtct ctgccggagt ctcggcttct
     1621 ccctcaaatt ttttcatctg aacagaagct ttaagaaccc tggccttcac tccatcggct
     1681 atttcgagaa tgaagctgtc ctctttgact tccctgacga tcccgaagaa gccccccgca
     1741 gtgacgacct gatcccccct gcgtatagag gcgatcattt cctcctgctt gcgctggcgt
     1801 ttcttctgag gccttattat caggaaatag aagatgacga cgaaaacacc caaaggcaac
     1861 agcatgccca taagattacc ctgtgcttcc aaaagatact cctccctctt gtggtatcag
     1921 gtactcgatt tttttcgcaa tgaacattct accatgagac agggacaatc ggaacagcaa
     1981 gaaggatccc gaagccccta gtcggagttc cccttgagga aaaacagcat tttttccagt
     2041 tccaccgata tgtcgaccct gcagacgagg acattctctg gaacagatat ggcagatgtg
     2101 gcgaaggtca gtatccccct tatcgtaccg gcggatattc ccttgtccac gctggactgt
     2161 gcggctgcgg ggggaactgt aagaacaagg acctctatac ccttttgtct gatgacttcc
     2221 gccatgtcag cagaatcata acagctaacc ccgcatatat cggtcccgat cttggcgggg
     2281 tcgttatcaa agagggcatc gatccggaat ttctcactcc tgagggcctt gtggccaaga
     2341 agggcctcgc caagcctccc gactcccaca agggctatct tccagagcct tttcggggcc
     2401 aggatgtcgc tgatatgctg gtagaggcgc tccacgtgat agccgactcc ccttttgccg
     2461 atctcaccaa aaaaggagag gtccttcctg acctggctcg ccttgagccc gagcatatca
     2521 cctatctcct gcgaagagac gaccctgcgc tcctcgttat gcaactgcat cagaagacgg
     2581 taataaagga ccaggcgctc aactgtcggt tcggctattc tcatcggatc tgccccctcg
     2641 ggattattgc cgacggaggt ctaccatggc ctctcgtcgc ctgtcacatt acgttccccc
     2701 accagttcaa atgcttccgt ccttcgcctt acgacggcct cttctacgga gactaccttt
     2761 atggtcagga tctttctcgg aaaagcgatc tcgacggtat ctcccggttt cagtaaccgc
     2821 gagggcctga ccgccatctc tcccaccctg accgcgccga gagatatcat ctcacgggcg
     2881 gtgctccttc ttttcacgat gtgagtcatc ttgaggaatc ggtcaattct caagtttgtg
     2941 cctccgggca caagcccagt gaatattata acaaatgatt tgcatcattg ctcatttcat
     3001 tgtcaaatag cggggaaaat atcgatcagt cgaggtagtc acgcagtttc ttgctcctgc
     3061 tggggtgtct gagtttcctc agcgcctttg cctctatctg ccgtatcctt tcccttgtca
     3121 cgccaaatct ccttccaacc tcctccagag tataggggtg gccgtcctca agaccgaatc
     3181 tgagcctcag gacctctttc tcccgctccg tcagttcctc gagcatccct tcgagctgct
     3241 cccggagaag ctgcaccgcc gcagcgtcgt cgggactggg cagattcttg tcctcgagga
     3301 aatcgctcaa ctggctgtcc tcttcctctc ctatcggagt ttcaagagag acaggctcct
     3361 gggcgatcct ctggatctcc tcaacacgat tggccgctat tgaaagttcc gccgctatct
     3421 cctcggcggt cggctcccgt ccgagcctct ggaccaactg tcttgatacc ctgataagtt
     3481 tgtttatcgt ctcgaccatg tgaacgggta tccttatcgt cctggcctgg tcggcgatgg
     3541 cacgcgtaat ggcctggcgg atccaccatg tcgcataggt gctgaatttg aaacccttgc
     3601 ggtaatcaaa cttctcgact gccctgatga gcccgaggtt gccctcctgt ataaggtcga
     3661 gaaagagcat tcccctgcct atgtatttct ttgcgatact gacgacaagt ctcaggttgg
     3721 cctcgaccag cttgctcttc ccgacaggat ccccggcctc tacaagtttc gccagttcaa
     3781 cctcttcctc cggcttgaga agggatatct tgcctatctc cctgagatac attctcacgg
     3841 gatcggaaag gggcagatcc tcgagtacgc ccatttcctc gtggtactcc gccgcgggcg
     3901 gggcatcggc gtcatgggca tcctgtttgg gtttgtccgt ctctaccaga tcgatgccaa
     3961 gttcctgcag gttgaagtaa aggctgtcca gggtttcctc gttcatcatt tcgccgggga
     4021 gatgcttttc gatgtcgtcg taggtcacga aacccttctc cctccccatg tggagaagtt
     4081 ccttgaaact ttcgatgtat cgaaggacct ctttcgaacc actttcggcc gcaacggtca
     4141 tatccccgtt tccgttctca gccttttcct ttccgggatc ggtcatggac tgccgactcc
     4201 tcctttcagt cttcttgcca gtgccagata ttccttcatc tcctgcgggg acgcctcact
     4261 gcgggcgtgc ctctctttca ggacggagaa cctgtccctg agacgcttcc tttccagtgc
     4321 cgagatcaca acctcccaga tttctccctc ttcgccgttc ttctcgcagt gggcccaccc
     4381 cgaggagatc attctcatcg gaaaagggtc ccccgtctcc gcccaccttg ttctgagttc
     4441 tcccgggctc tctcccgcaa gaagggacac ggcgatggtc ttggccctgt catccgagag
     4501 aagccccagg accgattcca ccgactcgga acttctcctt tttgagtcca cccagagaag
     4561 atagcaaaga gcagactccc atggatccac tgcgttgtct tccccggaag gatccgttcc
     4621 tactttatat acgctttccg cggccggaat gccttttttg ctccccggaa tatcttccct
     4681 tgcctgccgg agaagatcca gcatctcggg cagcaggatc ccgagaccct gggctacctg
     4741 tgttatatgc ctgttggcct ccagggccgg catctcggca agccccctta tgatctccct
     4801 tcgcgccgca gtccttttgg ctgggtcctc aagttcgctc ctcctgactc ttatgtgaaa
     4861 gaggggaaga ggttccgccc tctgcaggag agata
//
LOCUS       DAOPKQ010000098         4887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18805_length_4887_cov_4.335886, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000098 DAOPKQ010000000
VERSION     DAOPKQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18805_length_4887_cov_4.335886"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK146_04475"
     CDS             <1..728
                     /locus_tag="PK146_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPE91529.1"
                     /translation="RHPAGKRSGPDEPVGPWKVKALMDPSASVGKQGVKGAVLFDFDM
                     TLVDSSIGITYCLNRLALFFGLAEVSREEVVRTIGYPMVHAMEMLWGHFDPEWIDHYR
                     EHLVPLEYERMDAFPGTRETLSRLREEGLALGVVSNRKRLVPAVESSGLKEFFLSVVG
                     LEDVKNPKPDPEPVRLSLKILGSEAGEAFLVGDSEIDAEAARRSGVRFIGVTSGGRNR
                     EILLREGAWAVTDGIPGLTGILL"
     gene            778..1701
                     /locus_tag="PK146_04480"
     CDS             778..1701
                     /locus_tag="PK146_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91530.1"
                     /translation="MESKTATKVLYYFLTACIVVGAVLVYKAWWDRYFRLRPELAVAE
                     PVVIPLDVPLEGVLIWEEITLTSPADGTVSFPLGRGPVYCAKGDLIAEVEAAEGRRQV
                     KAPGPGYFIASLDGSEGSWTYGSIWPGSSRLPDPGPLRFLDDGQKVPSGGGIGKFIPQ
                     PQSLRCVAYLERSALDWDPSKRDYIEVKRSASDLPFRAEVRAVNDLGPTIKVYLTFPF
                     FSEKEIVSRRVSYLLHVKDARGVLIPESAVTLRERKKMVFLVTGDRSVAVEVTGVPVS
                     GRRFLVERGLAPGDLVIASGSDASEGEVRIW"
     gene            1698..2393
                     /locus_tag="PK146_04485"
     CDS             1698..2393
                     /locus_tag="PK146_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPE91531.1"
                     /translation="MRGIADNIDSLRERIARAAGKMGLDGDAVSIVAVTKTRSAEEME
                     EAALCGVDALGENRVQEALLKWDLSAPRSSVPWHLVGHLQRNKARKAAEIFSCIQSVD
                     SLELASALEKACLSLGRRMNVLLEVNIGEDPDKYGVSREDLPLLAEGLFERSTTLDLK
                     GLMTVLPLGLDERGTRSLFASMRELRDRLRSRLGIDLPVLSMGMSDDFEYAVMEGSTM
                     VRIGSAIFGRRKY"
     gene            2435..2932
                     /locus_tag="PK146_04490"
     CDS             2435..2932
                     /locus_tag="PK146_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPE91532.1"
                     /translation="MSDILTVLDVEKISFSKAMRGYNTEEVEEFLEKVAGSLQIYSEK
                     IKTLEVQLSRFDEQVREYTELKQSLQEALVLAQKSAEERVSSAESKAEVIIAEAEAQA
                     HKIVIGAKDRVDAHKRDIERLRQIRYQFVAEFKGLLAKFGSMLNSLEAPADESENEKE
                     REDCR"
     gene            2929..>4887
                     /gene="recG"
                     /locus_tag="PK146_04495"
     CDS             2929..>4887
                     /gene="recG"
                     /locus_tag="PK146_04495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPE91533.1"
                     /translation="MTEVLTRDSPVRFLKGVGPARAARLLRLGVQTIEDLLYLFPRRY
                     ENRGVAAPLASLQAGSFSSAVASVVAIERKPTRKRNLSLVTALLSDGSTLAQAVWFNR
                     RGLEKILLPGSKASFFGKVDRRGGFLQFTNPEFEIIAGEDGSPGQRTIVPVYPGTEGI
                     YQKWLRRLVSETIGSFPGDLTDPLPEFIREAKGLTPLETAISQMHFPTGREEWARARK
                     RLAFEELFLLQVALAIRKQGYMEDTEGKPLTWKGPLMERFLAEVLPFPMTGSQERVLS
                     ELARDSSGNIPMNRLLQGDVGSGKTVVALIFLLAAADSGFQGVIMAPTEILARQHHRR
                     LTDWLSALGVPVHLLVGSLPSSRREEILEEIASGEPCIVVGTHALIQKTVRFGSLAAV
                     VVDEQHRFGVLQRGALAGKGDHPHVLVMTATPIPRTLTLSVFGDLAFSVIDELPPGRI
                     PTVTRHLHDPDDPRLMKFIAKEMARGGRVYWVCPVIEESEKLPLMPVIRRHEILAERF
                     PEEKVGILHGQISPEEREETMEAFAGGDISILVSTTVVEVGLDVSEATVMVVEDAHRF
                     GLSQLHQLRGRVGRGSSAGSCFLVGRPSTDEGRARIEVMCSSSDGFRIAEKDLILRGP
                     GEICGVRQHGITDFRIADLVKDRRLLEEA"
BASE COUNT         1077 a         1249 c         1576 g          985 t
ORIGIN      
        1 aacgtcaccc ggctggcaag aggtctggac ctgatgaacc ggttggaccc tggaaagtga
       61 aggctttaat ggacccgtca gcctccgttg ggaaacaggg cgtgaaaggt gctgtcctct
      121 tcgatttcga catgaccctg gtggacagca gcataggtat tacctactgc ctcaacaggc
      181 tggccctttt cttcggtctc gccgaggtgt cccgtgagga agtggtcagg accataggat
      241 accccatggt ccatgccatg gagatgctat ggggtcactt cgaccctgaa tggatcgatc
      301 attaccgtga acacctggtc ccgctggagt atgaaaggat ggatgccttc cccgggacac
      361 gggagaccct gtccaggcta cgggaagagg gcctcgccct tggtgtggta tcgaacagga
      421 aaagactggt ccctgccgtt gagagttcag gtctgaagga gttctttctc tccgtcgtcg
      481 gtttggagga cgtgaaaaac ccgaagccgg accccgaacc cgtaaggctt tcactcaaga
      541 tccttggaag tgaagcggga gaggctttcc tggtaggcga ttccgagatc gatgctgaag
      601 cggcgagacg ttccggggtc aggttcatag gcgttacctc cggtggacgc aaccgggaga
      661 tccttctgag ggaaggggcc tgggctgtga cagatgggat ccctgggctg acagggatcc
      721 tgctctgaag agggaagggc tttaaaaaag ggtccggtgg agtgttatga taaaaacatg
      781 gaaagtaaaa ccgcgactaa agttctctac tacttcctca ctgcctgcat agtggtcgga
      841 gcggtccttg tatataaggc ctggtgggac cgttacttcc gcctccggcc ggagcttgcc
      901 gtggctgaac ccgtggtcat acccctcgac gtcccgctgg agggggtact gatatgggag
      961 gagatcaccc tgacatcgcc tgccgacggg acggtatcct tccccctcgg gagggggcca
     1021 gtctactgtg caaagggtga cctgatcgca gaggtggagg ctgccgaagg caggagacag
     1081 gtaaaggctc ctggtccggg gtatttcatt gcctccctcg acggatcgga gggcagctgg
     1141 acctacgggt ccatatggcc ggggagttcc aggctgcccg atccgggtcc gctccgtttc
     1201 cttgatgacg gtcagaaggt cccatcgggg ggcggcatcg gcaagttcat cccccagccc
     1261 cagtcgctga gatgtgtggc ctacctggag agatcagccc ttgattggga cccatccaaa
     1321 agggactaca tagaggttaa gagatcagcc tccgatctcc ccttcagggc tgaggtccgg
     1381 gccgtgaacg acctcggccc gaccatcaag gtctacctga cctttccgtt cttttcagag
     1441 aaagagatcg tttcgaggag ggtctcttac ctgctccacg taaaggacgc aaggggagtg
     1501 ctcatcccgg agagtgctgt caccctcagg gagaggaaga agatggtgtt cctggttaca
     1561 ggtgaccggt cagtagccgt tgaggtaaca ggtgtccccg tatccggcag acggtttctg
     1621 gtagaaaggg gcctggcccc gggggatctg gtgatcgcat ccgggtcgga cgcctcagag
     1681 ggggaggtaa ggatctggtg agaggtatcg ctgacaacat tgactccctc cgggagagga
     1741 tagcaagggc ggcaggaaaa atgggccttg atggagatgc tgtaagtatc gtggcggtaa
     1801 ccaagacccg ttctgcagaa gagatggaag aggccgctct ctgcggcgtg gatgcccttg
     1861 gagagaacag ggtgcaggaa gctcttttga aatgggatct ctccgccccg agatcatccg
     1921 ttccatggca tctcgtggga catctccaga ggaacaaggc aaggaaagcc gccgagatct
     1981 tcagctgtat ccagagcgtg gattccctgg agcttgcttc agctcttgaa aaggcatgtc
     2041 tttccctggg acgaaggatg aatgttctcc tggaagtgaa catcggagag gaccccgata
     2101 agtacggtgt atcaagggag gatctccctt tgttggcaga gggtcttttt gagagaagta
     2161 caactctcga tctgaagggt ctgatgacgg tcctccccct cggtctcgat gagaggggga
     2221 ccaggtcgct ctttgcatcg atgagggaac ttcgggacag gctgaggtcc aggttgggga
     2281 tcgatctccc ggtcctctcc atgggaatga gcgacgactt cgagtatgcc gtgatggaag
     2341 ggagcaccat ggtgaggatc ggaagcgcga tcttcggcag aagaaaatac tgaccccgtg
     2401 gcattttaag aggggcaggc ggggagggaa tgccatgtca gatattttga ccgttcttga
     2461 tgtcgaaaag atcagtttca gcaaggccat gagaggctac aacaccgaag aggtcgaaga
     2521 atttcttgaa aaagttgctg gaagcctgca gatctactct gagaagatca agaccctgga
     2581 agtccagctc tccaggtttg acgagcaggt cagggagtat acagaactta agcagtctct
     2641 gcaggaggcc ctggtgcttg cgcagaagag cgccgaggag agggtgagca gcgccgagag
     2701 caaagcggag gttatcatag ccgaggctga agcccaggcc cacaagatcg tcatcggtgc
     2761 caaggacagg gtggatgccc acaagcggga tatagagagg ctcaggcaga tcaggtacca
     2821 gttcgtagca gaattcaagg gacttcttgc caaattcgga tctatgctga actcgctgga
     2881 ggctccggcc gatgagagtg aaaacgaaaa ggagagggag gactgtcggt gacggaggtc
     2941 ctgaccaggg actctcccgt cagattcctg aaaggtgtcg ggcccgcaag ggctgcccgg
     3001 cttctcaggc ttggcgtaca gaccatcgag gacctcctgt atctcttccc ccgccgttac
     3061 gagaaccggg gagtagcagc gcccctggcg tcccttcagg cagggtcatt ctcctccgct
     3121 gtcgccagcg tagtggctat agagaggaaa cctacaagga agaggaatct gtccctggtg
     3181 acagctcttc taagcgacgg ttccaccctg gcccaggcgg tctggttcaa caggaggggc
     3241 ctggaaaaga tcctgctgcc gggctcaaaa gcttccttct tcggcaaggt ggacagaaga
     3301 ggaggttttt tgcagttcac caaccccgaa ttcgagatca ttgccgggga ggatggctcc
     3361 cctggccaga ggacgatagt ccccgtctac ccggggaccg agggaatata ccagaagtgg
     3421 ttgcgccgac tggtatccga gacaataggc tcctttccgg gggatctcac cgatcccctt
     3481 cctgaattca taagggaggc caaaggtctc acccccctgg agacggccat ctcccagatg
     3541 cacttcccta cggggagaga ggagtgggcc cgggcaagaa aaaggcttgc cttcgaggag
     3601 ctctttctcc ttcaggtggc cctggcgata agaaagcagg gctacatgga ggacactgag
     3661 gggaaacccc ttacgtggaa aggacctctg atggaacgat tccttgctga ggtccttccc
     3721 ttcccgatga ccgggtccca ggagagagta ttaagcgagc ttgcccggga cagctcgggg
     3781 aacattccaa tgaacaggct tctccagggg gatgtaggtt caggcaagac agtagtggct
     3841 ttgatattcc tgctggctgc agctgacagc ggtttccagg gagtgatcat ggccccgacg
     3901 gagatcctgg cgaggcagca tcaccggagg ttgacggact ggctatcggc actgggtgtc
     3961 ccggtccacc ttcttgtggg gtctcttcca agctcacgga gagaggagat cctcgaggag
     4021 atcgcgtcgg gggaaccctg tatcgtggtg gggacccacg ccctgatcca gaaaacggtc
     4081 aggttcggga gcctggccgc agtggtcgta gacgagcagc acaggttcgg ggtactccag
     4141 aggggggccc tcgcaggaaa gggagaccat ccccatgttc ttgtaatgac cgccactccc
     4201 ataccgagga ccctgaccct ctctgtcttc ggggacctgg ccttctccgt gatcgatgaa
     4261 cttcccccgg gaaggatacc aacggtgacc aggcaccttc acgaccccga tgacccgagg
     4321 ctgatgaaat tcattgcaaa agagatggcc cgggggggga gggtctactg ggtatgcccc
     4381 gtgatcgagg aaagcgagaa actccccctt atgcccgtaa taaggcgcca cgagatcctt
     4441 gcggaacgtt tcccggaaga gaaggtcggc atccttcacg gccagatctc tccggaggag
     4501 agagaggaga cgatggaggc gttcgcaggc ggagacatat cgatccttgt atccacgacc
     4561 gtcgtggagg tgggtcttga tgtgagcgag gcgacggtaa tggtggtcga ggatgctcat
     4621 cgtttcggcc tctcgcagct ccatcagctt agggggagag tggggagggg cagttcggca
     4681 ggttcctgtt ttctcgtggg aaggccgagt accgacgaag gcagggcccg gatcgaagtc
     4741 atgtgttcct catcggacgg gttcaggata gccgagaagg acctgatcct cagggggccc
     4801 ggtgagatat gcggagtaag gcagcatggc ataacagatt tccgtattgc cgatctggtc
     4861 aaggacaggc gccttttgga ggaggcg
//
LOCUS       DAOPKQ010000099         4855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_18958_length_4855_cov_4.544583, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000099 DAOPKQ010000000
VERSION     DAOPKQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18958_length_4855_cov_4.544583"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK146_04500"
     CDS             <1..406
                     /locus_tag="PK146_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91534.1"
                     /translation="DKTGRHRPGLQFAESSGLNVNRYRIIGSSISHAVGAVGIVVYAQ
                     SFGFIQLYMAPLFMAFFAVASILIGGASLKRATVTHAIIGTFLFQSILVVSMPVANVV
                     VADNIADVIRIIISNGIILYALTRKEIGGDVS"
     gene            403..1542
                     /locus_tag="PK146_04505"
     CDS             403..1542
                     /locus_tag="PK146_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91535.1"
                     /translation="MTSDSRGQSKKLALQETEAKAYKERIWKYYLRKNVVPIFFIVLC
                     FFGGYYSGLSLPFLINEIITRMARNSFLVISLIIPILAGMGLNFGIVLGAMAGQFGYI
                     ATANFQMAGFSGFVVTTLISIPLSILFGWLTGKLFNQAKGKEMITGIILGFFANGIYQ
                     LICLYFMGNFIPIRIPTMLLSTGIGLRNTLDIKSMHYALDNLWKIRINIPGLIFPIII
                     PVFTLLAVAVLCVAIHFLIKTKLGQEFRAVGQNRHIAEVSGIKVDKIRVIAIIISTVL
                     AGIGQVIFLQNLGNINTYGSHVQVGTFAVAAILIGGASVSKATIGQALLGTLLFHTLF
                     IVSPLAGKNLIGDAMIGEYFRVFVTYGVIGLALALHAWQARQKRR"
     gene            1559..2662
                     /locus_tag="PK146_04510"
     CDS             1559..2662
                     /locus_tag="PK146_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPE91536.1"
                     /translation="MCGSKRISLAHPLIKKSGFDALVVAPSPDLEYLTGLAPHPGERF
                     NGLFLMADGRSFAVVSRLYEQEYREGLPASAPVYEWSDEDWFFDALSQAFSDHGIGEG
                     AVIGVNDSVMAVDAVEIAQRQKVSLANGWHTLGGLRLVKDWEEIACLKKAGEISDRAL
                     EALLPFIKPGKTEKEIRRKLLELMDDFGATSQAFTPIVARGENAAMPHYAGGEGIIKE
                     KDSVLLDFGCRYRGYFSDMTRTFFIGERPEPEIEEMYQIVLRSQDKGESAARPGITSE
                     FLDMTARSVIEEAGYGKYFNNRLGHGIGLAIHESPNIMRGNQQTLAEGMTFSIEPGIY
                     IPGRIGIRIENCVVVTTDGCEPLTRFPKELRVL"
     gene            2677..4065
                     /locus_tag="PK146_04515"
     CDS             2677..4065
                     /locus_tag="PK146_04515"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HPE91537.1"
                     /translation="MTERDLLGKYESRIISNLQDMVRIRSVEGTPEEGKPFGDGVHQV
                     LNLALETASELGFRTVNHDGMIGYAEYGSGKEMVAVLGHLDIVPEGKGWTYPPFGAEI
                     HDGKMFGRGTIDDKGPTIAALWALKILSDEDHRLSRRIRIIFGTNEESGFRGIKWYAA
                     NEEAPVAGFTPDIHEGIVHAEKGGLHIGIDVPVGEHEGISLISFRAGEAVNIVPDQCE
                     AEFEGDPAALEKWAGVLRGALDSTPLGGEVVLEKRTLSAKVFGKAAHASLPEKGENAI
                     AALCRLIDANPPSHRPWEAFKVISGLLCDPRFGSDLGISMSDDVSGPLTCNPGLASFS
                     KGVLSVVLDIRHPVTVKGDLVVERMKEKLAPLGLTPVERSRNTPLFVPKDSPLIKDLS
                     EAFFETTGKHPKVMAIGGGTYAKALPNVVAFAPFPSGLPDLAHQADEYIGIEDLLSSV
                     LVVKAAMLRLAR"
     gene            complement(4116..4631)
                     /locus_tag="PK146_04520"
     CDS             complement(4116..4631)
                     /locus_tag="PK146_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE91538.1"
                     /translation="MRKRRSIRKFQDRPLTPEQIRVLEEAVLRSPSARNLRSWEFIFV
                     DDRDLLVKLADTRGSSSAFIAGAALGVVIIGDEKTVDTWVEDGSIAAIIIQLAAADLG
                     LGSCWAHVRNREHAPGKSAEDHVRELLGISDPLKVLCVIAVGYPAEQKPGIPKEELSS
                     GKIFRNRYGQV"
BASE COUNT         1092 a         1372 c         1314 g         1077 t
ORIGIN      
        1 cgataaaaca ggacggcatc ggcctggcct tcagttcgcc gagtcttccg gacttaacgt
       61 gaaccgctac aggatcatcg gctcctccat ctcccacgcc gtgggcgcgg taggtatagt
      121 ggtctacgcc caaagcttcg gtttcatcca gctctacatg gcacctcttt ttatggcgtt
      181 ctttgccgtc gcctcaatac ttatcggagg ggcgagcctc aaaagggcta cagtcactca
      241 cgccattatc ggcacatttc ttttccagtc catactggta gtgtcaatgc cggtcgccaa
      301 cgtcgtcgtg gcagacaaca ttgccgacgt tatcagaata atcataagca acgggatcat
      361 cctctacgcc cttacccgca aggagatagg aggtgacgtg agttgacctc cgacagcagg
      421 ggccagtcaa aaaaactagc gctccaggag accgaggcta aagcttacaa ggaacgtata
      481 tggaagtatt acctgaggaa gaacgtagtt cctatcttct ttattgtcct ctgtttcttt
      541 gggggctact acagcgggct gagtcttccc ttcctgataa acgagataat cacccgaatg
      601 gctcgaaaca gcttcctggt catttcgctg ataattccta tccttgccgg aatgggactc
      661 aacttcggga tagtcctggg agccatggcc ggacagttcg gatacatagc aaccgccaat
      721 ttccagatgg ctggcttcag cggctttgtc gtgacgaccc ttatctcgat ccccctttca
      781 atacttttcg gctggcttac gggcaaactt ttcaaccagg caaaaggcaa agagatgatc
      841 acggggataa ttctgggctt cttcgccaac gggatttacc agcttatctg cctttacttc
      901 atggggaatt tcatccccat aaggatcccg accatgttgc tttcaaccgg tataggtctc
      961 aggaacaccc ttgacatcaa gagcatgcac tacgccctgg acaacctgtg gaagatccgc
     1021 atcaatatcc ccgggctcat attccccata attatcccgg tattcaccct gctggctgtg
     1081 gctgttcttt gcgtcgcgat ccatttcctg atcaagacaa agcttgggca ggagttccgt
     1141 gcggttggac agaacaggca tatcgcggaa gtttcgggga tcaaggtcga caagataagg
     1201 gttatcgcca ttatcatttc caccgtcctg gcgggcatag gccaggtgat ctttctgcag
     1261 aacctgggca acatcaacac ctacggaagt cacgtccagg tagggacctt cgccgtagct
     1321 gccattctta tcggaggcgc ctcagtcagc aaggcgacca tcggacaggc cctgctcggg
     1381 accctccttt tccatacgct ctttatagtc tcgccactcg caggcaagaa ccttatcggc
     1441 gacgcaatga ttggtgaata cttcagggta tttgtaacct acggagttat cgggcttgct
     1501 cttgcccttc acgcctggca ggctcgccag aaacgccgtt aaataagggg gtccttccat
     1561 gtgcggatca aaaaggattt cactggccca cccactgatc aaaaagagcg gttttgacgc
     1621 tctcgttgtt gcaccgtcac ctgaccttga atatctgacc ggccttgccc cccacccggg
     1681 agagagattc aacggcctct tcctcatggc cgacggccgg agttttgccg ttgtctccag
     1741 gctttacgaa caggaataca gggaagggct tccagcttca gctcccgtct atgaatggag
     1801 cgacgaggac tggttctttg atgccctgtc acaggccttc tcagaccacg gtatcggcga
     1861 aggcgccgtt atcggcgtca acgactccgt aatggccgtt gatgccgtag agatcgccca
     1921 acgccagaaa gtcagccttg ccaacggctg gcacaccctt ggcggtcttc gccttgtcaa
     1981 ggactgggag gagatcgcct gcctcaaaaa ggcaggagag atatccgatc gggcactgga
     2041 ggccctgttg cccttcatca agcctggcaa aaccgagaag gagatcagaa gaaaactcct
     2101 ggagctgatg gatgacttcg gagccacttc ccaggccttc actcccatcg tggccagggg
     2161 ggagaacgcc gctatgcccc actacgcagg aggagagggt attatcaagg agaaggactc
     2221 cgttcttctg gatttcggct gccgttaccg cggctacttt tcggatatga ccaggacctt
     2281 cttcataggt gagaggccgg aaccggagat agaagagatg taccagatcg tgctgcgttc
     2341 ccaggacaag ggggaatctg ctgcccgtcc ggggatcact tcggagtttc ttgacatgac
     2401 tgcccgttcg gtgatcgagg aggcgggcta cggcaagtac ttcaacaaca ggcttggcca
     2461 cgggatcggc cttgctatcc atgaatcccc gaacattatg cggggcaacc agcagaccct
     2521 tgccgagggt atgaccttca gcattgagcc cggcatctac atcccgggga ggatagggat
     2581 aaggatcgag aactgtgttg ttgttaccac tgacggttgc gaacctctta ccaggttccc
     2641 caaggaactc agggtccttt gaagggtgtg agggcaatga ctgaaagaga tcttctggga
     2701 aagtacgaga gccggataat atcgaatttg caggatatgg taaggataag gagcgttgaa
     2761 ggaactcccg aggagggtaa acccttcggg gacggagtcc accaggttct caacctggcc
     2821 ctggagacgg cctccgaact cggtttccgg accgtcaacc atgacgggat gataggatac
     2881 gccgagtacg gctccggtaa ggaaatggtt gccgttctcg gtcatcttga catagtccct
     2941 gaagggaaag ggtggactta cccccccttt ggggctgaga tccatgacgg gaagatgttc
     3001 ggacggggaa ccatcgatga caaggggccg accatagcgg ccctctgggc cctcaagatc
     3061 ctctctgatg aggaccatcg gctatcaagg aggataagga tcatctttgg cacgaacgag
     3121 gagagcggat tcaggggaat taaatggtac gccgccaacg aggaagctcc cgttgcaggc
     3181 ttcactcccg acatccacga aggcatagtc cacgccgaga agggtggtct tcacatcggg
     3241 atagatgtgc ccgtcgggga acatgaaggg atcagtctga tctccttccg tgccggagaa
     3301 gcggtgaaca tcgtcccgga ccagtgcgag gccgagttcg aaggtgatcc tgcggccctg
     3361 gaaaagtggg cgggcgttct gcgtggagcc ctggattcca cacccctcgg gggggaggtg
     3421 gtactcgaaa agaggaccct ttcagcgaaa gtcttcggca aggcggccca tgccagtctt
     3481 cccgaaaagg gagagaacgc cattgcagcg ctctgcaggc tgatagatgc caatcccccc
     3541 tcccatcgcc cctgggaggc tttcaaggtc atctccgggc ttctctgtga cccaaggttc
     3601 ggatcagatc tggggatctc aatgtccgat gacgtctcgg gtcccctgac ctgcaacccc
     3661 ggtcttgcct ctttctcgaa gggtgtctta tctgtagtcc ttgacataag gcacccggta
     3721 accgtaaagg gcgaccttgt agtggagagg atgaaggaaa agcttgctcc cctcgggctg
     3781 accccggtag agagatcgag gaacactccc ctgtttgtgc ccaaggattc gcctcttatc
     3841 aaggatctca gcgaagcttt cttcgagaca acggggaagc acccgaaagt gatggccata
     3901 ggaggaggga cctatgcgaa ggccctcccc aacgtggtgg ccttcgcccc gttcccgtcg
     3961 ggtcttcccg acctcgccca ccaggcagac gaatacattg ggatcgaaga tcttctctct
     4021 tcggtccttg tcgtcaaggc cgccatgcta aggctggcaa ggtagggtac gaacaggatc
     4081 agagacaggg agggtgcaga cgcccctccc tttgttcaga cctggccgta cctgttcctg
     4141 aagatctttc cggaggacag ttcctctttc gggatcccgg gcttttgctc ggccgggtaa
     4201 cccacggcga tcacgcagag cactttcaga ggatccgaga tcccaagcag ctctctcaca
     4261 tgatcctcag cggattttcc cggggcatgc tctctgttac gcacatgagc ccagcatgac
     4321 cccagtccga ggtcagcggc tgcaagctgg atgataatgg ccgcgatgga cccgtcctca
     4381 acccaggtat cgacggtctt ttcgtcccca atgattacta ccccaagggc agcccccgct
     4441 atgaaagctg agctgctccc ccttgtatcg gccaatttga ccagcagatc cctgtcatca
     4501 acaaaaatga actcccatga cctcaggttc ctggcagagg ggctcctcag aacagcctcc
     4561 tccaggaccc ttatctgttc gggggtaaga ggtcggtcct ggaatttcct tatacttctt
     4621 cttttccgca aaaggtccag cacgaacatc atctctctct cttaaggggt aaatagccgt
     4681 cggaacgacg aaaaaagggg gcggtatata ccgcccccgt ctctttcatg gtcgggctct
     4741 ccggcataga gatgcacttc ccaggcaacc actccctaat ccgtcaacag ccttttgctg
     4801 ccaatttgca accagacctg acttccaact gaacccggtc aaactatccg ctgct
//
LOCUS       DAOPKQ010000100         4755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_19508_length_4755_cov_3.983191, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000100 DAOPKQ010000000
VERSION     DAOPKQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19508_length_4755_cov_3.983191"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PK146_04525"
     CDS             complement(<1..563)
                     /locus_tag="PK146_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPE91539.1"
                     /translation="MPAKFFYITTPIYYVNDVPHIGHAYTTIAADVMARYKRMRGFDV
                     MFATGTDEHGQKIQQAAEKRDMTAQQLVDSVMVNFKNLWKELEITNTDFIRTTEARHE
                     RVVQYIFSRLMEQGDIYKGSYKGWYCVPCETYFPESQIGGEKLCPDCGRPLQNMTEES
                     YFFRMSKYERPLLDYYEKNPDFILPPER"
     gene            complement(565..1401)
                     /gene="rsmI"
                     /locus_tag="PK146_04530"
     CDS             complement(565..1401)
                     /gene="rsmI"
                     /locus_tag="PK146_04530"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPE91540.1"
                     /translation="MPLVVIPTPVGNMEDITLRALRALRGADLIACEDTRRTMKILSR
                     YGIRTPLVSYHSFNEKERTRTLTERLSRGDTVALVSDAGTPGISDPGYELIRSSLEEG
                     FEVDVLPGATAFVPALLLSGFPPQPFLFEGFLSRRKGDRRRRLEELKSFTGSIVFYLS
                     PHGPVKILEDIHQVLGERPAVILREISKLHQERIPGSLASLQVLLGEASLKGEMVLVT
                     GPPPRRTEPESSGWQEMALGLCEQDLPDKEVVKRLSEEYGIAKNKIKQFLHSKRREKE
                     GL"
     gene            complement(1403..2128)
                     /locus_tag="PK146_04535"
     CDS             complement(1403..2128)
                     /locus_tag="PK146_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPE91541.1"
                     /translation="MKTTRDAILYGELVIEQPEDGPRVSVDSVLLASFVKAKGRERIL
                     ELGSAHGAVSLLLARRFPDTAIEGLEIQSELVAMAERNATDNGLSERVAFREGDLRRV
                     REIYGAQSFAVVVVNPPYDEVNHSRPSPLESEAAARHGTHCTLEDVIMASRYLLANKG
                     KLFIVFRAKRVAELLCLLSRERIEPKRIRPVYPAPGRDASVVLVQAARSAGRGAVLEP
                     PLFIQNEKGEYTGDLLAAYRIGD"
     gene            complement(2128..>4755)
                     /locus_tag="PK146_04540"
     CDS             complement(2128..>4755)
                     /locus_tag="PK146_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91542.1"
                     /translation="NRAEDAINSISEGRGSMASTVAGLERILTDSRRKLFSLSTSLVG
                     SMERRKALLGDIAGEKDRFAGTDKRLEDLERDVARAAEEEKGASRSFDDARREWEQYR
                     KRIEERSDRIRSLGEERAACEAALESARSKGKGFGLSLLDVLREISRSEESVKEARKD
                     LGRLREEILQKRSLEEAAGNRQRDVYAAFQQAAASLQKLSRELSALEGEMETLRETAF
                     TRVYPAPVQHLMAAVRLGRIDASPRPLADVISCPDRLVTALESFLGARQFLLFVKDID
                     EAGRCIAHLKARSAGRATFLPLESARARHPMRNIREGRQGIVGWASDLVGMESEWKEC
                     VLHVLGDLLIVESFDVARDLVREGFRGPAVTLEGDVFQPGGTISGGKTTKGAGAIEIR
                     RNLQEKEKQAESLKNERSRLEEEIAGLEKEERDASEKLRVLSEERRALEDRRRSGEAA
                     EAEAARARALADERRAGILAALSSCGREYLDIRSRLERLRESSSEEPETSRELDLVKS
                     LEESRSRAEVAAERLETARRMFTMVQDEHRRSSSRLEELDKGLEDLVLAEKSGKRELA
                     EEGRTYHGIWVRLQEALSGMKDLVEGHSRALASRERILERSASAAARMESLDSRMRQA
                     RQRLDSTEAEIAESIDMWEERFPYPGEESIDARDYDRTRRSVRELEKSLREIGDVDLG
                     VLSEDASLTERLGFLEEQLRDVSTGIDELRRLIEETDRQAGTLFSTSLKEIDRKFCDL
                     FQRLFGGGEAHLRLSDEDNLWEAGVDVVARPPGKRPQHLAQLSGGEQSLSAISLLFAA
                     MEVAGVPLAVLDEVDASLDEVNLRRFSELAKEYSRTMQLICMTHRRATMERADLMYGV
                     TMSEPGLSQVVGVRVEDWE"
BASE COUNT          811 a         1609 c         1271 g         1064 t
ORIGIN      
        1 cgctcgggcg ggaggatgaa atctgggttc ttctcgtagt agtcaagcag cggcctttcg
       61 tatttcgaca tgcggaagaa gtagctctcc tcggtcatgt tctgcagggg cctgccgcag
      121 tcggggcaga gcttctcgcc tcctatctgg ctctcgggga agtaggtctc gcaggggacg
      181 cagtaccacc ccttgtagga tcccttgtag atgtccccct gctccatgag ccggctgaag
      241 atgtactgta ccacccgctc atgcctcgcc tcggtggtcc ggatgaagtc tgtgttggtt
      301 atctccagtt ccttccagag gttcttgaag ttgaccatca ccgaatctac gagctgctgg
      361 gcggtcatgt ccctcttctc tgcggcctgc tggatcttct gaccgtgctc atcggtgccg
      421 gtggcgaaca tgacatcgaa gcctcgcatc ctcttgtacc tcgccatcac atcggccgca
      481 attgtggtat aggcatggcc tatatggggg acatcgttca cgtagtagat cggggtcgtt
      541 atgtagaaaa attttgccgg catttcaaag gccctccttt tcgcgcctct tgctgtgcag
      601 gaactgcttg atcttgtttt tcgcaatacc atactcttcc gagagcctct tgacaacctc
      661 tttgtcgggg aggtcctgct cacacagtcc aagggccatc tcctgccagc cactgctctc
      721 cggctcagtc cggcggggcg ggggacccgt gaccaaaacc atctccccct tcagggatgc
      781 ctcgcccagg aggacctgga gggaggccag acttccgggt atcctctcct ggtgcagctt
      841 ggagatctct ctcaggatca ccgccggcct ctctccaagg acctggtgga tatcctcgag
      901 tattttcacc gggccatggg gtgagagata gaagacgata ctccctgtaa agctcttcaa
      961 ctcctccagc cttcgccttc gatccccttt cctccttgag aggaagccct cgaacaggaa
     1021 gggctgtggg gggaaccccg acagcaggag agcggggacg aaggctgttg ctcccggcag
     1081 gacatcgacc tcgaagccct cctccaggga gcttctgatc agctcatacc cggggtcgga
     1141 gatcccgggc gtgccggcat cggatacgag ggccactgta tctccccggg agagcctctc
     1201 cgtaagggtc ctggtccgtt ccttctcgtt gaaggagtgg taggagacaa ggggggtcct
     1261 tatcccgtac ctggagagta tcttcatcgt tcttctggtg tcctcgcagg ctataaggtc
     1321 cgctcccctc agggccctca gggccctgag agttatgtcc tccatgttgc ccaccggcgt
     1381 ggggatcaca acaaggggca tctcagtcac ctatcctgta ggctgcaagg aggtcccccg
     1441 tatattcacc cttttcgttc tggatgaaca gggggggctc cagcactgct cccctgcctg
     1501 cagaccttgc agcctgaacg aggaccaccg aagcatccct tccgggagcc gggtagacgg
     1561 gcctgatcct cttgggctcg atccgctccc tggagaggag gcagagaagc tcggccaccc
     1621 tcttggccct gaagacgatg aagagcttgc ccttgttggc gagaagatac ctggaggcca
     1681 tgatcacgtc ctcaagcgtg cagtgcgtgc cgtgcctggc ggccgcttcg gactccagag
     1741 ggctggggcg gctgtggttt acctcgtcgt agggcgggtt caccaccacc actgcaaagg
     1801 attgggcccc gtagatctcc ctcactctcc ggaggtcacc ctccctgaat gccacccttt
     1861 ctgaaaggcc gttgtcggtg gcgttcctct ccgccatggc gaccagttcg gactggatct
     1921 caagcccctc gatagcggtg tcggggaacc tcctcgccag aagaagcgac acagccccgt
     1981 gggctgaacc aagctcgagg atcctctccc tccctttggc cttgacgaaa gaggcgagaa
     2041 gaacgctgtc cacgctcacc ctgggcccat cctcaggctg ttcgatcacc agctctccgt
     2101 agaggatagc gtcacgggtc gtcttcatca ctcccaatcc tccaccctta cacccaccac
     2161 ctgtgagagt cccggctcag acatggtgac gccgtacatc aggtcggccc tttccatggt
     2221 tgccctgcgg tgggtcatgc atatcagctg cattgtcctg gagtattcct tggccagttc
     2281 ggagaaacgc ctgaggttca cctcgtcaag ggatgcgtcg acctcgtcga gaaccgccag
     2341 gggcactccg gcaacctcca tggcggcaaa gagaagggat atagccgaca gcgactgctc
     2401 cccccccgaa agttgggcca ggtgctgggg tcttttcccc ggcggtcggg cgaccacgtc
     2461 cactccggcc tcccagaggt tatcctcgtc ggacagcctg aggtgtgctt cccctccacc
     2521 gaagagccgc tggaaaaggt cgcagaactt cctgtctatc tccttaagcg aggtggagaa
     2581 tagcgtacct gcctgcctgt ccgtctcctc gataagcctc cttagttcgt ctatccccgt
     2641 cgagacgtca cggagctgct cctccaggaa gcccagcctc tccgtgaggg atgcgtcctc
     2701 cgaaaggacc cccaggtcaa catcccctat ctcccgcagg ctcttctcga gttccctgac
     2761 gcttctcctt gtcctgtcgt aatccctggc atcgatgctc tcctcaccgg ggtaggggaa
     2821 cctctcctcc cacatgtcga tcgactccgc tatctccgcc tctgtggaat caagtcgctg
     2881 tctcgcctgg cgcatcctgg aatccaggct ctccatcctg gccgcggccg aggctgatct
     2941 ctccaggatc ctctcccttg aggccagggc cctggaatgt ccttccacaa ggtccttcat
     3001 tcccgacaga gcctcctgga gtctcaccca tatgccgtga taggtccttc cctcttcagc
     3061 gagctctctc ttgcccgact tctcggccag gacgaggtct tcaagaccct tgtccaactc
     3121 ctcgagacgg gatgaggatc ttcggtgctc atcttgcacc atggtgaaca tcctcctggc
     3181 cgtctcaagc ctttcggctg ccacctccgc cctggacctg ctctcctcga gggacttgac
     3241 cagatcaagc tcccgggagg tctcaggctc ctcggaagag ctctctctca gcctttccag
     3301 cctggaccgg atatcaaggt actcccttcc gcaggaggag agggcggcca gtatccccgc
     3361 tctcctctca tcagcaaggg cccgggccct tgcggcctca gcctcagcag cttccccgga
     3421 ccgccttcgg tcctccaggg ccctcctctc ctctgacagg accctcagct tctccgaggc
     3481 atccctctct tccttctcaa gccccgcgat ctcctcctca aggcgcgacc tttcgttctt
     3541 caggctctcc gcttgcttct ccttctcctg gaggttcctc ctgatctcaa tggcaccggc
     3601 acctttggtg gtctttcccc cgcttattgt tccgccgggc tggaacacat ccccctccag
     3661 ggttaccgcc ggcccccgga acccctccct tacaaggtcc ctggccacgt caaaactctc
     3721 cacgatcaga aggtcaccga ggacatgaag aacgcactcc ttccactcgc tctccatgcc
     3781 taccaggtcc gaggcccatc cgacaattcc ttgccggcct tcccttatgt tccgcatggg
     3841 gtgcctggcc ctggcgctct ccagggggag aaaggtcgcc ctgcccgccg acctggcctt
     3901 gagatgggca atacacctcc ccgcctcgtc gatgtccttc acgaagagga ggaactgcct
     3961 tgccccgagg aaggactcca gggcggtcac aagcctgtca ggacaggaga tgacgtcggc
     4021 cagcggcctg ggggaggcgt ctatccggcc aagccttact gcggccatca ggtgctgtac
     4081 cggggctgga tagacccttg taaaggctgt ctccctgagg gtctccatct caccctcaag
     4141 ggcggatagc tccctggaaa gtttctggag cgatgcggcc gcctgctgga aggcggcata
     4201 gacgtccctc tgcctgttgc cggccgcctc ttccagggac ctcttctgca ggatctcctc
     4261 cctgagccgt ccgagatcct tcctggcctc tttcacgctc tcttcggaac gggatatctc
     4321 cctgagcaca tccagtaagg aaaggccaaa cccctttcct tttgaacggg ctgattccag
     4381 ggctgcctca caggcggccc tttcctcccc aaggctcctt atcctgtcgg acctctcctc
     4441 gatcctcttt cgatactgct cccattccct cctggcgtcg tcgaaggacc gggaggcccc
     4501 cttctcctct tctgccgccc tggccacgtc cctttcaagg tcctccagcc ttttgtctgt
     4561 cccggcaaaa cgatccttct ctccggcaat atcccccagc agggctttcc ttctctccat
     4621 cgatcccacg agggacgtag agagggagaa aagtttcctc cgggaatccg tcaggatccg
     4681 ctcgagtcca gcgacggtgg aagccatgga gcccctgccc tcggagatcg agttgatcgc
     4741 atcctcggcc ctgtt
//
LOCUS       DAOPKQ010000101         4639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_20225_length_4639_cov_4.071117, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000101 DAOPKQ010000000
VERSION     DAOPKQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20225_length_4639_cov_4.071117"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK146_04545"
     CDS             <1..220
                     /locus_tag="PK146_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE91543.1"
                     /translation="GMDPIDVCSLLDRRGICAWDGHFYAIRPIEVLGLLERGGVTRVG
                     VSLYNTADEVQRLLEAVADIAEGKLRKE"
     gene            225..1259
                     /locus_tag="PK146_04550"
     CDS             225..1259
                     /locus_tag="PK146_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPE91544.1"
                     /translation="MRVLFIGFGNVGRTVARILSRERSRFPGLEVLDGLVTAGIVTRR
                     GGAMVSSEGIDLDLALKDMETLGCFNEKEGSFCRITAEEAASTLDYDVLVELSTLSIE
                     NRGEPAATHIRTALKRGRHAVSANKGPVAFAYRELSELARNAGREFLFETTVMDGAPV
                     FNLSRSCLGGCVINAVDGILNSTTNFILGRMEEGAGFEKAVREAQETGVAEADPGHDV
                     DGWDAAAKVAALANVLLEGEITPLEVERKGIRNIGIDRIRKAACSGRRLKLICRAWRD
                     QGGVHGKVGVEEVPMDHPFALVKGGGAVLRFHTDLMGPVLVTQEKPDLYDTAYGVLGD
                     LVRISGAFRS"
     gene            1331..1831
                     /locus_tag="PK146_04555"
     CDS             1331..1831
                     /locus_tag="PK146_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPE91545.1"
                     /translation="MTRGKTMKNLEEAFSGESQANRKYLAFAKKADDEGFHQIARLFR
                     ATAEAETIHAHTHMRSMGMIGTTAENLDAAIAGETYEFKSMYPAFIEDAVAEGKKQAE
                     RSFRLANEAEKVHAGLYEKARETLGSGDSFDYFLCTVCGHIAEGGAPDSCPLCGAKKQ
                     AYRLVE"
     gene            1961..2956
                     /locus_tag="PK146_04560"
     CDS             1961..2956
                     /locus_tag="PK146_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HPE91546.1"
                     /translation="MYKIRVLNSEDLKKLLDIRDVIPAVETAYEQYSRGEAELFPVIA
                     HEFESGLREMDIKSGFLSRTGLAGLKVIGYVADNPDKRRIPAVSGLVMVLSIETGRPV
                     GILDGMVITEIRTGAAGAVGARHLARKGSREVLIVGPGAQGRAQLHGLMKVLSGIEVV
                     RIAGRDPEKLSRFVNEMSSLYPGVSLSAVRMEDIQGAVSSSDVIVTCTPAHEPYIMKD
                     WVRPGTHINAIGADFSGKQELDEDLLPLSRLVADSKVQTLEIGEMQTAFRKGLVRESD
                     VIEIGSVISGDVVGRRSEEEITVFDSTGMALQDIVTANLALVRAIERGIGSLVTM"
     gene            2979..3962
                     /locus_tag="PK146_04565"
     CDS             2979..3962
                     /locus_tag="PK146_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HPE91547.1"
                     /translation="MFTAPEMKDVLGAARFLEGKIRRTPVEMSIPLSEAARSEVFLKL
                     ENLQLCGSFKVRGALNKMYSLSGPEKDRGVITASSGNHAQGVAMAANLLGVRAVICVP
                     GSCPQTKRDAILSRGKGMVELRVIGRFYDEAEEEAKAAAGREKLVYVSAYEDHHIVCG
                     QGTLGLEFLLDVPDLDLLIVPISGGGLIAGVAIAARALRPGIEVVGVHASANPSWKVA
                     WEKEEVVPQEESETYADALSGAASRLLFPVIREVVSGVAEVSECEIARAISFMHSRHH
                     QVVEGAGSVGVAAVLAGKVDIAGRKTGIIISGGNIGDATLLKAVAEAGDMD"
     gene            3959..4174
                     /locus_tag="PK146_04570"
     CDS             3959..4174
                     /locus_tag="PK146_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91548.1"
                     /translation="MSDQGEEKRKNMEDRGHSPLTVILFFTVTLALAVKLMINIPFFR
                     EFFSGYGKFILLPVLLFLAYIGIHRKG"
     gene            complement(4159..>4639)
                     /locus_tag="PK146_04575"
     CDS             complement(4159..>4639)
                     /locus_tag="PK146_04575"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPE91549.1"
                     /translation="VQTMDPSGLDWLGRGHGTSEVIDALARSSRRRIFTCAHLISGIP
                     GEKKGQLAASSLQLSDLGVDALKFHPLYVLTGTRLEALFRSGTFVPQEVEEYVHEVIE
                     AIRHIPDRVILQRISADASPPELVSPSWISQKSMVLSLVQEGMKSLGARQGDLYPFR"
BASE COUNT         1065 a         1147 c         1467 g          960 t
ORIGIN      
        1 tgggatggac cccatcgatg tctgctccct tctcgaccgt aggggtatct gcgcctggga
       61 cgggcacttc tatgccatcc gcccgatcga ggtcctgggt ctccttgaaa gaggaggggt
      121 caccagggtc ggcgtatcac tttacaacac ggccgacgaa gtccagagac ttctagaagc
      181 agtagcagat attgccgaag gcaagttgcg aaaggaataa aatcatgagg gtcctcttca
      241 tcggcttcgg gaatgtagga agaaccgttg caaggatcct ttcccgggaa aggtcccggt
      301 ttcccgggtt ggaggtgctc gacggcctcg ttacggcagg catagtcacc agaagaggag
      361 gcgccatggt ctccagtgag ggaatcgacc tggacctggc ccttaaggac atggagaccc
      421 tcggatgttt caatgagaag gaagggagct tctgcaggat cacagccgag gaggctgctt
      481 ccacccttga ttacgatgtg cttgtggagc tttccaccct ttcgatagag aacaggggtg
      541 aaccggcggc gacccatatc agaacggcat tgaagagagg ccgccatgca gtttctgcaa
      601 acaaggggcc cgtagctttt gcctacaggg agctttcgga acttgcgaga aacgccggaa
      661 gggaattcct cttcgagaca acggtcatgg acggcgcacc cgtcttcaac ctgagcaggt
      721 cctgccttgg agggtgcgtt attaatgctg ttgacgggat attgaactct acaaccaact
      781 tcattcttgg aaggatggag gagggagccg gcttcgaaaa ggctgttcgg gaggcccagg
      841 agacgggggt ggccgaggcg gaccccggtc acgatgttga cggttgggac gcagccgcca
      901 aggtggccgc tctggcgaac gtacttctcg aaggcgagat cacccccctt gaggtcgaaa
      961 gaaaagggat ccggaatatt ggtatcgaca ggataaggaa ggcagcctgt tcgggtcgca
     1021 gactgaagct gatctgtcgt gcctggagag atcaaggggg ggtccatgga aaggtcggag
     1081 tcgaggaggt tccgatggat caccccttcg cgctggtgaa aggcggtgga gcggtactca
     1141 ggttccacac ggacctgatg ggacccgttc tcgtgaccca ggagaaaccc gatctgtatg
     1201 atactgccta cggtgttcta ggagatctgg tacggatctc gggtgctttc agatcatgac
     1261 agattaaccc ttgaaaggaa gagcctccgc tgatatattt gaaaggattt taaaaaagga
     1321 gggggtagag ttgaccagag ggaagaccat gaagaacctc gaggaggctt tcagcggtga
     1381 gtcacaggcc aaccggaagt acctggcctt tgcaaaaaaa gcagacgatg agggttttca
     1441 ccagatagcc aggcttttcc gggccactgc cgaggcggag acgatccatg cccatactca
     1501 catgcgctcc atggggatga taggaactac tgcagagaac cttgatgccg ccatcgccgg
     1561 tgagacctat gagttcaaga gcatgtaccc ggctttcatc gaagatgcag tggctgaggg
     1621 aaagaagcag gcagaaaggt ccttcaggct tgccaacgaa gcggaaaagg tccatgcggg
     1681 tctatacgag aaggcaagag aaactcttgg ttcgggggat agcttcgact atttcctctg
     1741 caccgtctgt ggccacatcg ctgaaggagg ggcccctgac tcatgcccgc tctgcggggc
     1801 gaagaagcag gcctaccgac tggttgaatg aggcacctaa agaagctact gtagtgtccc
     1861 gagtccgcaa aagggggccc tcagaggggt cccctttttg ccggtggggg agatagggta
     1921 tagtattccg gaaaggaccg cccccggaag gagtgatgaa atgtacaaga taagggttct
     1981 gaacagcgaa gaccttaaga aattactgga tatcagggac gttattcccg ccgtggagac
     2041 ggcctacgag cagtactccc ggggtgaggc cgagcttttt cctgtaattg cccatgagtt
     2101 cgagagcgga ctccgggaaa tggacatcaa gtctggcttc ctttccagaa caggtctggc
     2161 ggggctcaag gtcatcggct acgtggcaga taaccccgac aagaggagga tccccgccgt
     2221 atcggggctt gttatggtcc taagcatcga gaccggaaga cctgtaggga tcctggacgg
     2281 tatggttatt accgagataa ggacgggagc cgctggcgcc gtgggagcca ggcaccttgc
     2341 aaggaaaggg tccagagaag tgcttatcgt cggtcccggg gcccagggca gagcccagct
     2401 ccatgggttg atgaaagtcc tttcaggtat agaggtggtg cggatcgccg gcagggaccc
     2461 ggagaagctc tccaggttcg ttaatgagat gtcttccctt tacccggggg tgtccctttc
     2521 ggcagtccgg atggaagata ttcagggggc tgtctcctcg tcggacgtga tagtgacctg
     2581 cactcctgcc cacgaaccgt acatcatgaa ggattgggtc cggccgggga cccacataaa
     2641 cgctatcggg gcagactttt cggggaagca ggaactggac gaggatctac tccccctttc
     2701 ccgtctcgtt gcggacagca aggtccagac ccttgagata ggtgagatgc agaccgcttt
     2761 caggaagggt cttgtcaggg aatccgatgt gatcgagatc gggagcgtga tctccggtga
     2821 tgtggtcgga agaagaagcg aagaggagat aaccgtcttt gactcaactg gcatggcgct
     2881 ccaggatatt gtgaccgcca atctggccct tgtcagggca atagaacgag ggatcggttc
     2941 acttgtgacc atgtgacgga agaaggaggg atccctttat gttcacagcg cccgagatga
     3001 aagatgttct gggagctgca agattcctgg aaggaaagat caggagaacc cctgttgaga
     3061 tgtcaatccc cctttcggag gctgcacggt ctgaagtgtt cctcaagctg gagaacctcc
     3121 agctctgcgg ttcattcaag gtaaggggcg cgctcaacaa gatgtactct ctttccgggc
     3181 cggagaagga tagaggcgtc atcacggcct cgtccgggaa ccacgcacag ggagtggcaa
     3241 tggccgctaa ccttctgggt gtgagggctg tcatctgcgt ccccggttcg tgcccccaga
     3301 caaagaggga tgccatcctc tccagaggta agggaatggt ggagctgaga gttatcggcc
     3361 gcttctacga cgaagcagag gaagaggcca aggccgcagc tggaagagag aagctcgtat
     3421 acgtctccgc ctacgaggat caccatatcg tctgcgggca gggcaccctc ggtcttgagt
     3481 tcctgttgga cgtgcccgac ctggaccttt taattgtccc cattagcggt ggagggctta
     3541 tcgccggtgt cgccatcgct gccagggccc tcagaccggg tatagaggta gttggagtac
     3601 atgcctcggc caacccttca tggaaagtgg cttgggaaaa ggaagaggtc gttccccaag
     3661 aggagagcga aacctatgcg gacgcccttt ccggggctgc ttccaggctc ctcttcccgg
     3721 tgataagaga ggtcgtgtcg ggagttgcgg aagtgtcgga gtgcgagatc gcaagggcca
     3781 tttctttcat gcattccagg caccatcagg tggtggaggg tgcaggctcg gttggtgttg
     3841 ccgctgtcct ggcgggcaag gtcgatattg cagggaggaa gaccggaata ataatcagcg
     3901 ggggcaatat cggagatgcc acactcctga aagccgtagc agaggcaggt gatatggatt
     3961 gagcgaccaa ggagaagaga aaagaaaaaa catggaggac agggggcatt cgcccctgac
     4021 ggtgatcctg ttctttaccg taacactggc gctggcggtt aagttaatga tcaatatccc
     4081 tttttttcgg gagttctttt cgggctatgg caagttcatt ctgcttcccg ttctgctttt
     4141 tcttgcctac ataggcattc accgaaaggg gtaaagatcc ccctgccggg ccccgaggct
     4201 tttcatacct tcctgtacaa gggacaggac catggatttc tggctgatcc atgacgggga
     4261 aaccagctcc gggggggagg cgtctgcaga gatcctctgg aggatgaccc tgtcaggtat
     4321 gtgtctgata gcttcgataa cctcatgaac gtactcctcg acttcctgcg gaacgaaggt
     4381 ccctgaccgg aaaagagcct caagcctggt cccggtgagg acgtacaggg ggtggaactt
     4441 gagggcgtcc acaccgaggt ccgaaagttg cagagaggag gcagcgagct gccctttttt
     4501 ttcgccgggg atcccggaga taaggtgggc gcaggtgaat atccttcttc tggaagacct
     4561 ggcaagagca tcaatgacct cggatgtccc gtggcctctt cccagccaat ccagccccga
     4621 gggatccatg gtctggaca
//
LOCUS       DAOPKQ010000102         4630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_20283_length_4630_cov_4.404153, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000102 DAOPKQ010000000
VERSION     DAOPKQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20283_length_4630_cov_4.404153"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK146_04580"
     CDS             <1..216
                     /locus_tag="PK146_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPE91550.1"
                     /translation="GGIARPGDALSMILAGASAVEVGTALFIDSGLPASICRELEANL
                     TKKGLASVRELVGRARNDQGENGEKSR"
     gene            213..938
                     /gene="pyrF"
                     /locus_tag="PK146_04585"
     CDS             213..938
                     /gene="pyrF"
                     /locus_tag="PK146_04585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPE91551.1"
                     /translation="MTRKDGPGLILAVDVKRPAEAERILDLLKENLRYVKIGPRLFAA
                     GGMQFVRRVMRAGFRVFLDLKLHDIPNTVAEAVDLFARKGIWALTLHASGGEEMLRAA
                     VSAKAGVGSSLMLLGVTVLTSMDQISLDMISPGSALQGTILSRARLCEDCGLEGIVCS
                     PRDLPLVVPRSTDRLVTVVPGIRDGQGVQDDQKRTASLEDALQMGADYVVIGRPIVRA
                     PSPLDAMSAYSERLERWRENNGY"
     gene            928..1515
                     /gene="pyrE"
                     /locus_tag="PK146_04590"
     CDS             928..1515
                     /gene="pyrE"
                     /locus_tag="PK146_04590"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPE91552.1"
                     /translation="MDINHPVSITALMKEAGALQEGHFALSSGLHSAHYLQCALFLMY
                     PENAALAGSLLAEKLSEPRPSLVVSPAMGGVIIGHEVARSLGVPFIFCEREKGSMKLR
                     RFPSPGTARYVIVEDVVTTGRSTLETKDLMDGLSGTSFLGAGCIADRSGGTTGLGSDL
                     VSLVTFDFPNYSREDCPLCREGVPLSEPGSRRLSL"
     gene            1562..2404
                     /locus_tag="PK146_04595"
     CDS             1562..2404
                     /locus_tag="PK146_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91553.1"
                     /translation="MENVSRKKGPGEWSRVILLALIMVIILFGSKCEALEVWVTNPWL
                     SMLTRFIGGVQVTVQPVVTWGEKGEMVKARKIPPTGAVVITLDPVEGTRLLGKGFEGK
                     FQVFFLFDRMPSVTDVDESFMDPAALPFIGLRILQILSSCKPGEYDYFQRRLAEFQAR
                     LDSTVIVGRNMIGESAILDLCWKYGRWLQAASGRTVRPPSHVKEEWSRSEAMNVLDTA
                     LDEARRQGWIVAVDPWTPDVVRERLGENPRYIELPPPRMEEDFILFLYDQYLRIWDFL
                     RRNP"
     gene            complement(2409..3380)
                     /locus_tag="PK146_04600"
     CDS             complement(2409..3380)
                     /locus_tag="PK146_04600"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPE91554.1"
                     /translation="MAVLKELIDAGGQTVSGGAVCRSLGLSRQAVWKAVKALREDGYT
                     ISSVPVKGYRFVSSPDNDLEPSWIEAVLFDCPWGHPVLYWKSLDSTQTQAKDLARKGA
                     REGLVVTTGYQVSGRGRLGRKWVSAPEGGIYFSVVIRPALPPESIQVLSLVSAMSVQD
                     AILDICGVKCQLKWPNDILWEGAKLCGILTEVSSEPGLVHFAVTGIGINANSRGMPLG
                     DGAEATSLSSIIGRRVHRGEIVAGVVRALNRAVRELESDNGVGRALAEYSSRCDTLGR
                     KVRVVFDGGEVTGEAVALGEKGEIVVRTDNGTRSFTSADVNHLRAFD"
     gene            3514..4618
                     /gene="prfB"
                     /locus_tag="PK146_04605"
     CDS             join(3514..3585,3587..4618)
                     /gene="prfB"
                     /locus_tag="PK146_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583336.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPE91555.1"
                     /translation="MAQMSMTTVMEGLHSLASELKDSLDPAVLFAGIERLTEVTSDPD
                     FWTGEDPQSVTKELARLQSKLDSWKDISAELDELQTMVDLLNAEEDPELEREFHERAS
                     LLEKRIHDLQLLFLFDEEYDESNAILSIHPGAGGHDSQDWAEMLLRMYLRWAEAKGFR
                     TRILEMIEDEEAGIKSATIHVAGEYAYGYLKTERGVHRLVRMSPFDTAKRRHTSFASL
                     NVSPELPDDVEIEINPEDLRIDTYRSGGAGGQHVNMTDSAVRITHLSTGIVVSCQNER
                     SQHMNRQSAMQVLRGRLYERMLQERQEQINAIQGEKKEIGWGSQIRSYVLHPYTLVKD
                     HRTNLEKGSVQAVLDGDIDEFILAFLRFRKHGK"
BASE COUNT         1039 a         1214 c         1354 g         1023 t
ORIGIN      
        1 ggtggtattg caaggcccgg tgatgccctt tccatgatac ttgcaggtgc ctccgcagtc
       61 gaggtcggaa cggctctgtt cattgattcc ggccttcccg catctatctg cagggaactg
      121 gaagcgaacc tgacgaaaaa gggacttgca tcagtgagag aactggtcgg gagggcccgt
      181 aacgatcaag gggaaaatgg agaaaagtcc cgatgacgag aaaagatggt ccgggcttga
      241 tcctggccgt ggatgtgaag aggcccgccg aagccgaaag gatcctggat ctcctcaagg
      301 agaacctccg ttatgtaaag ataggcccaa ggcttttcgc tgcggggggg atgcaattcg
      361 taaggcgagt catgagggcg gggttcaggg tcttcctgga cctgaaattg catgacattc
      421 ctaacaccgt agcagaagcg gtggaccttt ttgcccgtaa aggcatctgg gcgctgaccc
      481 ttcacgcttc gggaggcgaa gagatgctca gggccgcggt atcagcgaag gctggtgtcg
      541 gttcatcctt gatgctactg ggtgtaacgg tcctgaccag tatggaccag atatcccttg
      601 acatgatatc tcccgggtca gcgctgcaag ggacgatcct gagccgggcg cggctctgtg
      661 aggattgcgg tcttgaaggt atagtctgtt ctccgagaga tcttcccctc gttgtccctc
      721 gttccacgga caggctagtc acggttgtcc ccgggatcag ggacggtcag ggagtccagg
      781 acgaccagaa gaggacggcc tccctggaag atgcgctgca gatgggggca gactacgtcg
      841 ttatcggaag acccatcgta agggctccat cgccgttgga cgccatgtcc gcatattctg
      901 aacgtcttga gcgctggagg gaaaataatg gatattaacc atccggtaag catcacagcc
      961 cttatgaaag aggctggagc cctgcaggaa ggtcattttg ctctctcctc cgggcttcac
     1021 agcgcccact acctccaatg cgcccttttc ctgatgtacc ccgagaatgc ggcccttgcc
     1081 ggttcccttc ttgcagaaaa gctctctgaa cctcgaccgt ctctcgttgt ctctcctgct
     1141 atgggcgggg tcatcatagg acatgaggtg gccaggagtc tgggcgtgcc ctttatcttt
     1201 tgcgaaagag agaagggttc gatgaaactt agaagatttc cttcccctgg cactgccaga
     1261 tacgtcatcg tagaagatgt ggtcaccacg ggaagatcga cgctggagac aaaagatctg
     1321 atggatggtc tttcagggac ttcttttctg ggtgccgggt gtattgccga ccgcagcggc
     1381 ggcacgaccg gtctggggtc ggatctggtt tcactggtca cttttgattt ccccaactac
     1441 agcagggagg attgccccct ctgcagggag ggtgttcccc tttccgagcc tggcagccgg
     1501 aggctttcgc tctgaggccg ggaaaggttt cgatctgtct ttacaggggg catcttgttt
     1561 gatggaaaat gtatccagga aaaagggacc cggggagtgg tcgcgggtca ttcttctggc
     1621 cctgataatg gttattatcc tttttgggag caaatgtgaa gctttagagg tctgggttac
     1681 caacccctgg ctttccatgc tgacccgttt cataggtgga gtccaggtga cggtccaacc
     1741 ggtggtgaca tggggggaga agggggagat ggtaaaggcc cgaaaaattc ctccgaccgg
     1801 agcggtcgtt atcacgctgg accctgtgga ggggacgcgg cttctcggaa agggttttga
     1861 aggaaaattc caggtctttt ttctgttcga caggatgcca tcagtaacag acgtggacga
     1921 gagcttcatg gatcctgcag cccttccctt tatcgggctc cggatacttc agatactatc
     1981 ttcctgcaag cccggagaat acgactattt ccagagaagg ctggccgagt ttcaggcccg
     2041 gctcgacagt actgtgatcg tcgggaggaa catgatagga gagagcgcta tccttgatct
     2101 ctgctggaag tacggaagat ggctgcaggc tgcttctggc aggacggtaa gacctccttc
     2161 ccatgtcaag gaggagtggt cccgttcaga agccatgaat gttctcgata ccgcccttga
     2221 tgaagcgaga agacagggct ggatagttgc agtggaccca tggacaccag acgtggtgag
     2281 ggagaggctg ggggagaacc ccagatacat agagctcccc cctccaagga tggaggaaga
     2341 cttcattctc tttctttatg accagtacct gaggatctgg gactttctta gaaggaaccc
     2401 ctgagatttc agtcaaaggc cctgaggtgg ttcacgtcag cggaggtaaa ggaacgggtc
     2461 ccgttgtcgg tcctgacaac gatctcacct ttttctccca gggccactgc ctctccggtc
     2521 acctctcctc cgtcaaagac gacacggacc tttcttccca gggtgtcaca tcttgaggaa
     2581 tattcggcaa gggctctccc gaccccattg tccgactcca gttcccttac agccctgttg
     2641 agggcccgga ccacgccggc gacgatctct cccctgtgaa ctctcctgcc gataatggat
     2701 gaaagggatg tggcttcagc cccgtccccg agaggcattc cccgggagtt ggcgttgatc
     2761 cctatccctg tgaccgcgaa atggaccaga cctggctcac tggatacttc ggtaaggatc
     2821 ccgcaaagct ttgctccctc ccagaggatg tcgttgggcc atttaagctg gcacttcact
     2881 ccacagatgt ccagtatcgc atcctggacc gacatggccg acaccaggct gaggacctgg
     2941 atagactcgg gaggtagtgc cgggcggata acaacggaga aatagatccc gccctccggt
     3001 gccgagaccc actttctgcc aagtcttcct cttccggaga cctggtaacc ggtagttact
     3061 acaaggcctt ccctggctcc cttccgcgcg aggtcttttg cctgtgtctg ggtggagtcc
     3121 aggctcttcc agtagagtac aggatgcccc caggggcagt cgaaaagaac agcctctatc
     3181 caggagggtt ccaggtcgtt gtccggtgag gagacgaacc tgtaaccctt taccgggacg
     3241 gaactgatgg tgtatccgtc ttcccggagg gccttgaccg ccttccatac agcctgccgg
     3301 gaaaggccca gggatctgca tacagctcca ccggaaactg tctgtccacc cgcgtcgatc
     3361 agctctttca ggacagccac cttcgtgtta aggacgggat ccataggctt gagtcttgcc
     3421 ccctcgttga ggttcacgtg atgatataat gaaatgcctg caactgtaaa tgataacact
     3481 cccgggcgat gatgacattt ggaggggatc gacatggctc agatgtccat gactacagta
     3541 atggagggac ttcactcgct tgcttccgag ttgaaggaca gcctttgacc cggccgtact
     3601 gtttgccggg atcgaaagat tgacggaggt cacatccgat cctgacttct ggacaggtga
     3661 agatccccag tccgtaacca aggagctggc ccgtcttcag tcaaagctgg attcctggaa
     3721 agatatctct gcagagttgg atgaacttca gaccatggtc gatctcctta acgcagagga
     3781 agaccctgag cttgaaagag agttccacga acgggcatct cttttggaga agaggatcca
     3841 cgaccttcag cttcttttcc tttttgatga ggagtacgat gaatccaacg cgatcctgag
     3901 cattcacccc ggcgcgggag gccacgattc acaggactgg gccgagatgc tcctcaggat
     3961 gtaccttcga tgggccgagg cgaagggttt cagaacacgg atcctggaga tgatcgagga
     4021 cgaggaggcc gggatcaagt cggcaacgat ccatgtggct ggagaatacg cttacggcta
     4081 cctgaagacg gaaaggggtg tccacaggct ggtaagaatg tccccctttg acacggccaa
     4141 gaggagacat acaagctttg cctctctaaa tgtctcaccg gagctgccag atgacgtgga
     4201 gatcgagatc aaccccgagg atctcaggat cgatacctat cgatcagggg gggccggggg
     4261 gcagcatgtg aacatgaccg attcggcagt aaggatcacc catctgtcga cgggtatcgt
     4321 cgttagctgc cagaacgaac gctcacagca catgaaccgc cagagcgcca tgcaggtact
     4381 caggggacgt ctttacgaaa gaatgctgca ggaaagacag gagcagatca atgccattca
     4441 gggagagaag aaagagatcg gctggggcag ccagataaga tcctatgtcc tacacccata
     4501 cacattggtg aaggatcacc gcacaaacct ggaaaaaggc agtgttcagg cagttctgga
     4561 cggcgacatc gatgaattca tcctcgcctt tctccggttc aggaagcacg gaaagtgatc
     4621 aattctcgga
//
LOCUS       DAOPKQ010000103         4630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_20286_length_4630_cov_3.874536, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000103 DAOPKQ010000000
VERSION     DAOPKQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20286_length_4630_cov_3.874536"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /gene="lpxI"
                     /locus_tag="PK146_04610"
     CDS             complement(<1..762)
                     /gene="lpxI"
                     /locus_tag="PK146_04610"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300499.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HPE91556.1"
                     /translation="MKRTIGLFAGDGELPVEIARRLSGEGNPAVAFSFREDTKPLRKW
                     TSEVIKVDRPAIGKIVKDLEARGIRDLILAGLVPKKLIYRADLMDDDLRNIISTLSSR
                     DDHAVLGAVVSFFESRGIRVLPYREIVPEMLAREGIIAGRRPLPEEEDDILYGLRIAS
                     FLVPMSFGQTVVIKGRSVVSVEAMEGTDAAIERAGAISRGGVVVKMMRPDQDERFDIP
                     VIGPGTLETMHEADIGCLAVEAGRTIVLGGELFRER"
     gene            complement(774..1919)
                     /gene="lptG"
                     /locus_tag="PK146_04615"
     CDS             complement(774..1919)
                     /gene="lptG"
                     /locus_tag="PK146_04615"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HPE91557.1"
                     /translation="MTRTMKGETPSTAASRRIAASLLDRFIVSQMGNSFVFGVMVFSV
                     LLVAGDLLFQIANLMIDKGVALGVVLRLFVYKLPEVVVMTLPMASLLSALLTFGRLSS
                     QSEIVALRAAGVSFLRIVKPVLFASVLVSLGAILLNETIVPLSNKAAENIMRYEIARE
                     RPTLLREKVFLRDEDGGELRRVIYISRLRQNLGTMEDVLVQEFESGKMNRIVTAARGT
                     WGDGEWWLEDGKTFEVMEDRTVKLLFTFARQKLPLELGPTEVAQTSQRPAEMSAWQLW
                     GHISVMSKQGANLAPLWVLFHLKLALPWACVILAVLGASLGVGPQRSGAGVGLAMSVL
                     IVFAYYVLMSFSRSFGEAGYLPPFVAAWAPNLVFLVISGSLVRIADR"
     gene            complement(1916..2395)
                     /locus_tag="PK146_04620"
     CDS             complement(1916..2395)
                     /locus_tag="PK146_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPE91558.1"
                     /translation="MIRLFVMDVDGTMTDGAVFLDGEGREFKRFDVKDGLGVAMLAAS
                     GVRAAIISGRYSAVTDRRAKELGIDIVVQGVKDKLSELKRMASDMNLDPEEIAYIGDD
                     VNDRECIAWAGRGFAPSDARHEAKEAADVVTEARGGSGAVRDAADDILNFNGTRETK"
     gene            complement(2388..3191)
                     /gene="lpxA"
                     /locus_tag="PK146_04625"
     CDS             complement(2388..3191)
                     /gene="lpxA"
                     /locus_tag="PK146_04625"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPE91559.1"
                     /translation="MSFFVHPTSIVSKEARIADGVWIGPFCMVGSDVEIGEETRLEGH
                     VRILDHVTLGKRCRVFENVVLGGEPQDLAFRGELSFVRIGDDVTLRENVTIHRGSGEG
                     TVTSVGSGTWMMVGSHAGHNASIGEGCILANGVGLSGYSSVDDGSVLGGMSGLHQFTR
                     VGRSCMVGGLTKVVKDVPPFTLVDGHPARIHGLNVVGLRRKGFSQEDRLQVRRAYREI
                     FLSSSIDREALARLEQDKSLCTYVKEILEFIHGSKRGITPWGSRRGHDD"
     gene            complement(3188..3634)
                     /gene="fabZ"
                     /locus_tag="PK146_04630"
     CDS             complement(3188..3634)
                     /gene="fabZ"
                     /locus_tag="PK146_04630"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPE91560.1"
                     /translation="MLDIHRIFESLPHRYPFLLVDRITEISEERVVGYKNVTINEPFF
                     QGHFPSEPVMPGVLILEAMGQVGGMLISQRPELKGSVFYLTSVEKARFRRPVVPGDQL
                     VTEATIEKIRGRVGKVRTVARVGKDIAAEAVLGFVIASRLTGEEAE"
     gene            complement(3654..4475)
                     /locus_tag="PK146_04635"
     CDS             complement(3654..4475)
                     /locus_tag="PK146_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPE91561.1"
                     /translation="MKVQRIKSEARFRGVGIHSGCPSEVVVTPSESPGLFFSLPGGVF
                     PVQSAQTDGTARSTTLVFPDGSRVMTVEHLLAALSGLGVWSALLSLSGPEMPAMDGSA
                     KVFSETLAPLCEPEGDITPLTVTREIAFGSPDGGYIGVIPSDTFEITCSIKYDVPTIG
                     SQVFEGVMSRDTFIEAIAPARTFVLASEIGEIRKRGLGRGGALDNVLVIEDEAPLPSQ
                     VFRVPCEPVRHKVLDLMGDLATLGVPLRGRIVAYRSGHRMHLELVRRIRRGLLKD"
     gene            complement(4450..>4630)
                     /locus_tag="PK146_04640"
     CDS             complement(4450..>4630)
                     /locus_tag="PK146_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91562.1"
                     /translation="VPPGAIVSGFPARDHREQMKIQALIARLPGMREEIRKLKKEIAD
                     LKTRGGHCEGPENKE"
BASE COUNT          993 a         1491 c         1183 g          963 t
ORIGIN      
        1 cctctctctg aacagttcgc ctccaagaac gatggtacgg cccgcctcta cggcaaggca
       61 gccgatatct gcctcatgca tcgtttccag agttcccgga ccaatgacgg ggatgtcgaa
      121 cctttcgtcc tgatcggggc gcatcatctt gaccaccact ccaccccggg agatggcacc
      181 ggccctctcg atggctgcat cggtgccctc catggcctcc acggaaacaa cagatcttcc
      241 cttgatcacc accgtctgtc cgaaggacat cgggaccagg aacgaggcta tcctcagacc
      301 gtacagaata tcatcctcct cttcgggaag gggtcgtctc ccggctatga tcccctcccg
      361 tgcaagcatt tcggggacta tctcccggta ggggaggacc ctgatccccc ttgactcgaa
      421 gaaggatacg acggcaccaa gaacagcgtg gtcgtcccgc gagctaagcg tcgagatgat
      481 gttccgcaga tcatcatcca tgagatcggc ccggtagatc agcttcttgg gcaccagccc
      541 cgcaaggatg aggtccctga tgcccctggc ctcgagatcc ttcacgatct tccctatcgc
      601 cggcctgtcg acctttatta cttccgaagt ccactttcgc agcggtttcg tatcttccct
      661 gaaagaaaag gccactgcag gattaccctc tcccgagagc ctgcgggcga tctcgacagg
      721 cagttcgccg tcacccgcaa atagtccgat cgttctcttc aacgaagaca cccctatctg
      781 tcagctatcc tcaccagaga tcctgaaatg accaggaata caaggttcgg ggcccaggct
      841 gccacaaagg ggggcaggta gcccgcctct ccgaaagaac gcgagaagga catgagcaca
      901 tagtaggcga aaactatcag tacgctcata gccagcccga ccccggcacc cgacctctgg
      961 ggacccaccc ccagggaggc tcccagaacg gcaaggatca cgcatgccca gggcagtgcc
     1021 agtttcagat ggaacaagac ccacagggga gccaggttcg ctccctgttt gctcataacg
     1081 ctgatgtggc cccagagctg ccaggcgctc atctccgcgg gtctctggga ggtctgggcg
     1141 acctccgtcg ggcccagttc aagggggagc ttctgccttg caaaggtgaa gagcagcttg
     1201 acggtccggt cctccatcac ctcgaaggtc ttaccgtcct cgagccacca ttctccatcc
     1261 ccccaggttc cccttgcggc agtgacgatc ctgttcatct tgccgctctc gaactcctgt
     1321 accaggacat cctccatggt ccccaggttc tggcgcagac gcgaaatgta gatcaccctt
     1381 cggagctctc ctccgtcctc atccctcagg aacaccttct cccggagcag tgtaggtctt
     1441 tcccgagcga tctcgtagcg cattatgttc tcggccgcct tgttactaag gggcactatc
     1501 gtctcattca gaaggatcgc tcccagtgag acgagaaccg aggcaaaaag caccggtttt
     1561 acgatcctga ggaatgaaac acctgcggcc cgaagggcaa cgatctcgct ctgggatgag
     1621 agccgaccga aggtcagcag tgccgacaaa agcgaagcca tgggaagggt cataacgacc
     1681 acttcgggga gtttatatac aaagaggcgc agcactacgc ccagggcgac ccccttgtct
     1741 atcatcaggt tggcgatctg gaagagaagg tctccagcca ccagcaggac agagaagacc
     1801 atcactccga agacaaagga attccccatc tgggagacaa tgaacctgtc gaggaggctt
     1861 gcggcgatcc tgcggcttgc cgcagtggag ggagtttcac ctttcatggt cctggtcatt
     1921 tcgtctccct tgttccgttg aaattgagga tatcatcagc ggcatcacgg acggcgccgg
     1981 aaccgccccg tgcttcagta acaacatcgg cggcctcttt tgcttcatga cgcgcgtccg
     2041 aaggagcgaa gcccctcccc gcccaggcta tgcattcccg atcgttcaca tcatcaccta
     2101 tataggcgat ctcttcagga tccaggttca tatccgatgc cattctcttc aactccgaaa
     2161 gcttgtcctt cactccctgg acaactatat ctattcccag ttccttcgct cgcctgtccg
     2221 tgacggcaga gtatcggcca cttattatgg ccgccctgac ccctgaagcc gccagcatgg
     2281 ccactcccag cccgtccttg acatcgaaac gcttgaactc tctcccctcg ccatcgagga
     2341 agaccgctcc gtcggtcatg gtcccgtcta cgtccattac gaaaagccta atcatcgtga
     2401 cctctcctgc ttccccaggg cgtgataccc cgtttcgaac cgtgaatgaa ctcgaggatc
     2461 tccttgacat aagtgcacag ggacttgtcc tgctccagcc ttgccagggc ctccctgtcg
     2521 atggaggaag agaggaagat ctcacggtag gcccgtctca cctgaagtcg atcctcctga
     2581 gaaaaaccct ttcttctaag cccaaccaca ttgagtccat ggatcctggc cgggtgcccg
     2641 tccacaagcg tgaagggtgg gacatctttc accaccttgg tgagacctcc taccatacag
     2701 gatcgaccca ccctggtgaa ctggtgaaga cctgacatcc ccccgaggac actgccatcg
     2761 tccacgctcg aatacccgga gagccccacc ccgttcgcaa ggatgcaccc ttcccctatg
     2821 gaggcgttgt gcccggcgtg ggaacccacc atcatccagg tgccacttcc gacagaggtt
     2881 acggtacctt caccgctccc tctgtggatg gtcacgttct ccctcagggt cacgtcgtca
     2941 ccgatcctga caaaggagag ctctcccctg aaggcaagat cctgcggctc ccccccaagg
     3001 acaacgttct cgaagaccct gcatcttttc ccgagggtta cgtgatcaag gatcctgacg
     3061 tgaccctcaa gccttgtctc ctcgccgatc tccacgtcgg agcctaccat gcaaaagggg
     3121 cctatccaga ctccgtcggc gatccttgcc tcctttgaaa ctatcgatgt agggtggacg
     3181 aaaaagctca ttcggcttct tctccagtca gtctggacgc tatcacgaaa cccagtacag
     3241 cctcggcggc aatatccttt ccgactctgg caacggtacg gaccttgccg acacgtcccc
     3301 tgatcttctc gatcgtcgcc tcggtcacca gctgatcccc gggcacgaca ggtctgcgga
     3361 aacgtgcctt ttcgacggag gtcaggtaga aaacagagcc tttcaactcc ggacgctggg
     3421 atatcagcat tccgcccacc tgacccatgg cttccaggat caggacaccg ggcatgaccg
     3481 gttccgaggg aaagtggccc tggaaaaagg gctcgttgat ggtcacgttc ttgtacccca
     3541 caaccctctc ttcgcttatc tcggttatcc tgtccacgag aagaaagggg tagcggtgag
     3601 gaagggactc aaagatcctg tgaatatcga gcatgatcgt tctcctttcc cgttcagtct
     3661 ttgagcaggc cccggcggat ccgtcgcacc agttcaaggt gcattcggtg acctgacctg
     3721 taggcaacga tccttcctct cagaggtacc ccgagggtgg caagatctcc catcaggtcc
     3781 aggaccttgt gccgtaccgg ttcacagggc accctgaaca cctgagatgg aagaggagct
     3841 tcatcttcga tgacaaggac gttatccagg gcgccgccgc gtccaagccc tcttttcctg
     3901 atctccccta tctccgaagc aagcacaaag gtcctggcag gcgcaatggc ctcgataaag
     3961 gtatccctgg acatgacccc ctcaaagacc tggctgccta tggtcggaac atcatactta
     4021 atggagcagg ttatctcaaa tgtatcagag gggatcactc caatgtaacc tccgtcgggt
     4081 gatccgaagg cgatctcccg ggtcactgta agaggtgtga tatccccttc gggttcacag
     4141 agaggtgcca gggtctcgga aaagacctta gcgctcccgt ccatggccgg catctccggt
     4201 cccgatagcg acagcagtgc actccatacc ccaagcccgc tcagggctgc aagaagatgt
     4261 tccacagtca tcacccggga accgtctggg aacaccagcg tggtgcttct tgcggtcccg
     4321 tcggtctgag cggactggac aggaaagacc cctcccggga gagagaagaa gagcccaggg
     4381 ctttccgaag gggtcaccac cacctcggag ggacatcccg aatggatacc cactccacgg
     4441 aagcgggcct cactctttat tctctggacc ttcacaatgg ccgccccttg tctttagatc
     4501 agctatctcc ttcttcagtt ttctgatctc ctctcgcatc ccaggcaatc tcgctatcaa
     4561 agcctggatc ttcatctgtt cgcggtggtc cctggcaggg aacccggaga cgatggcacc
     4621 ggggggaacg
//
LOCUS       DAOPKQ010000104         4601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_20476_length_4601_cov_4.268368, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000104 DAOPKQ010000000
VERSION     DAOPKQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20476_length_4601_cov_4.268368"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PK146_04645"
     CDS             <1..696
                     /locus_tag="PK146_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91563.1"
                     /translation="FLSVSVGANQVVSGLAVTMFGTGASSLLGRKMIGLTVKGFGKVP
                     VPLLSEIPVAGPVLFDHDPLVYISYGLVLFLLWFIFRTRQGLNLRAVGESPVVVDAMG
                     LSAARLRYLYTMIGGGMAAVGGAYLSLAYTKMWGEGMSAGRGWIAVALVIFAVWHPAR
                     AVLGAYLFGGVGAMQLRIQAAGTSFPAPIMMMMPYLFTILVLLVISVKKGKGILIDAP
                     GALGVPYSREERT"
     gene            complement(715..1224)
                     /gene="gpt"
                     /locus_tag="PK146_04650"
     CDS             complement(715..1224)
                     /gene="gpt"
                     /locus_tag="PK146_04650"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HPE91564.1"
                     /translation="MQGTDNRYKKTYPVSWSQFHNDCRALAWRLLEIRADWERIIAIA
                     RGGLVPAAIIARELDIHFIDTVCVSSYTVRSQGKSKVLKNVLDDSERSLIIDDLVDTG
                     KTARIVRNMLPESHFATVYAKPDGRPLTDTYITEVSQETWILFPWDSEPQYQYIDPIA
                     DLSRVRQKG"
     gene            complement(1265..2665)
                     /gene="cysS"
                     /locus_tag="PK146_04655"
     CDS             complement(1265..2665)
                     /gene="cysS"
                     /locus_tag="PK146_04655"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPE91565.1"
                     /translation="MALSLFNDLTRRKETFVPIEPGKVRFYSCGPTVYDFFHIGNARP
                     FIVFDALRRFLEAKGYEVTYVQNFTDIDDKMINRAAELGISVPELADRFIAAYFEDAD
                     ALGVRRPTHSPRATMFIPEIIDLVSRLVENGHAYVVDGDVYFDVKSFPDYGKLAKQSL
                     DDLLSGARIEVDPRKRHPLDFVLWKNRKEGEPSWKSPWGEGRPGWHIECSAMSMNLLG
                     ETIDIHAGGSDLVFPHHENEVAQAEAATGKPFVNYWIHNGYILMDQEKMSKSLGNFLT
                     ARKARERFSPFAIRLFMLGAHYRSPISFSDESLRQAESATARIRNSYTELVNAPIDPA
                     TDTSGPADFPDDALERFDAALEDDFNTAGAIGVLFEVIRIVNQATAGGKSVAANYRER
                     ALSFLSYVNGILGLFDMSGNGEEALAEEVEKLIAEREKARSIRDFKRADSIRDELASR
                     NIILEDTPHGTRWKRS"
     gene            2836..4314
                     /locus_tag="PK146_04660"
     CDS             2836..4314
                     /locus_tag="PK146_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPE91566.1"
                     /translation="MNTSGLESKIASLPLRPGVYLFLDEEDNVIYVGKAKSLRKRVQS
                     YFRHGDFASPRLRKLVASIRDMSFIRTETEAEALITEARLIKQYQPFFNIDLKMGERY
                     PYVKITSEPFPRVVITRHREEGDGSEYIGPFVSAGELRHMLRLVERFFPIRVCRAGSG
                     NGQWAKRPCVRYELGRCPGPCAGLADPSEYRERVADIALLLRGRSADLVGRLRQRMDK
                     ASALMDFETAARMRDTIRALWKLSRKHVSMVFSEPLDGGTWKSLVQLQDLLSLPAIPW
                     RIEGFDVSHFSGGEAYGVCVVFEQGMPNPSLYRRYRIRTVEGVDDFRAMGEILGRRYT
                     RLLKGSGAFPQLILVDGGPGQLEFAVKALNTIGISDIPVVAIAKREELLFTPGAGGAL
                     RLPLDSGALMLLRRVRDESHRFAIAANRKAGRKRMSLSALEEVAGVGKILAARLLSKF
                     GSVARMSSLTPEEIASVKGVSIKTARSIIVHLGGPEAEDADM"
     gene            4301..>4601
                     /locus_tag="PK146_04665"
     CDS             4301..>4601
                     /locus_tag="PK146_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPE91567.1"
                     /translation="MLTCDPKVDAYYMGAALSLALRGTGRVSPNPMVGCVIVGPEGVV
                     GWGFHEGPGEPHAEAVALDRAGEKARGATLYVNLEPCCHQGRTPPCAPRIIKTGIA"
BASE COUNT          941 a         1207 c         1423 g         1030 t
ORIGIN      
        1 tttctctctg tttcggtggg agccaaccag gtagtgagcg gtctggccgt aaccatgttc
       61 ggaaccggtg ccagctcgct tctgggcagg aagatgatag gactgaccgt gaaaggcttc
      121 ggaaaggtcc cggtgcctct cctctcggaa attcccgtgg ccggtccggt actcttcgac
      181 cacgaccccc tggtctacat ctcctacgga cttgttcttt ttctcctttg gttcatcttc
      241 cggacccggc aggggttgaa cctgagggct gtgggtgagt ctccggtagt tgtggatgcc
      301 atgggactga gcgcagccag gctgaggtac ctttacacca tgataggggg agggatggcc
      361 gccgtcgggg gggcctacct ttcgctggcc tataccaaga tgtggggcga gggaatgagc
      421 gccggccggg gctggatcgc agtggccctg gtgatcttcg ctgtctggca ccctgcacgg
      481 gccgtccttg gggcctatct cttcggaggg gtaggggcaa tgcagcttcg gatccaggct
      541 gcggggacaa gctttccggc accgataatg atgatgatgc cctatctttt caccatactg
      601 gtcctgctcg tcatatccgt caagaaaggg aaggggatac ttatagacgc cccgggagct
      661 ctcggggtcc cctactcccg ggaggagagg acctgaggcc atcatggggg tctctcatcc
      721 cttttgccgg acccttgaga ggtctgcaat ggggtctatg tactggtact ggggttccga
      781 atcccagggg aagaggatcc acgtctcctg gctgacctcc gtaatgtagg tgtcagtcag
      841 gggtctgccg tcgggcttgg cgtagacggt ggcgaaatga ctttcaggga gcatattcct
      901 gacgatcctg gcagtcttgc ccgtgtctac aaggtcatct attatcaggg atctttcgct
      961 gtcgtcaagg acattcttga gcacctttga tttcccctgg gacctgacgg tgtagctgga
     1021 gacgcagact gtgtcgatga agtggatgtc cagctccctg gctataatgg ctgcaggaac
     1081 aagtccgccc ctggcgatgg cgatgatcct ctcccagtcg gcccggatct ccagcagtct
     1141 ccaggccagg gccctgcagt cgttgtggaa ctggctccag gatacagggt aggtcttctt
     1201 gtagcggttg tcagtccctt gcatgctctt cctcccagat ctcaaaaggt catcccgggt
     1261 ctcttcagga cctcttccat ctcgtgccgt ggggtgtatc ttccagtatt atgttcctgg
     1321 aagccagttc atccctgatg gaatcggccc ttttgaagtc tcttatactc ctggccttct
     1381 ccctttccgc gataagtttc tccacctctt ccgcaagggc ctcttcacca ttcccggaca
     1441 tatcgaagag gccaagtatt ccgttcacat aactgaggaa cgagagagcc ctctccctgt
     1501 aattggccgc tacgcttttg cctcctgccg tcgcctggtt gacgatccgg atgacttcga
     1561 aaagcactcc aattgctccg gcggtgttga aatcgtcctc cagggcggcg tcgaaccttt
     1621 caagggcatc atcggggaag tctgctggcc cggaagtatc cgtcgcggga tcgatggggg
     1681 cgttcaccag ttctgtgtag gagttcctga tcctggcggt ggcactttca gcctgcctga
     1741 ggctctcgtc ggagaagctg atcggggatc tgtagtgggc gccgagcatg aagagcctga
     1801 tcgcaaaggg tgagaacctc tcccgggcct ttcttgccgt gaggaaatta ccgagggact
     1861 tggacatttt ttcctggtcc atgagtatgt atccgttatg aatccagtaa ttcacgaagg
     1921 gtttccccgt tgccgcctcg gcctgggcca cctcgttctc gtgatggggg aagaccaggt
     1981 cgctgccgcc ggcgtggatg tctatggtct cgccaaggag gttcatggac atggcgctgc
     2041 attcgatatg ccaaccgggt ctcccctctc cccaggggct cttccaggat ggttcaccct
     2101 ccttcctatt cttccagagg acgaagtcaa gggggtggcg cttcctggga tccacctcga
     2161 tacgggcccc ggagagaagg tcgtcaaggg actgcttcgc cagcttcccg taatccggga
     2221 aggatttaac atcgaaatag acatctccgt ccacgacgta ggcgtggcca ttctcgacaa
     2281 gccgggaaac gaggtcgatg atctccggga taaacattgt tgccctcggg gaatgggtcg
     2341 gacgccttac tcccaaagca tcggcatcct cgaagtatgc tgcgatgaac cggtcggcaa
     2401 gttccggaac ggagatgccg agctcggccg ccctgtttat catcttgtcg tcgatatcgg
     2461 tgaagttctg gacgtaggtg acctcgtagc ccttggcctc gagaaaacgc cggagggcat
     2521 caaagacaat gaagggtcgg gcgttgccga tatggaagaa atcgtatacc gtgggtccac
     2581 aggagtagaa ccttaccttt ccaggctcaa tggggacaaa ggtctctttt ctgcgggtaa
     2641 gatcgttgaa aagacttaaa gccatgacgg cacctccagt gaaagagaag gatcctctat
     2701 tgaggtatcc tataccaaga ggtcacggcg gacaacagtt cagtcccttt ccggtcacgg
     2761 gtcaaggggg gtttatcaaa accttctttg ggaatagact atcggggatc cggatattta
     2821 gatgggggag agagattgaa tacctccggt ctggaaagca agatcgcttc gctgcccctt
     2881 cgccccggag tctacctttt cctggacgaa gaagataacg ttatctacgt aggtaaggcg
     2941 aagtccctga gaaagagggt ccagtcctat ttcaggcatg gagatttcgc gtcgccccgg
     3001 ctgaggaaac tcgtggcatc gatcagggac atgtccttca taaggacgga gaccgaggcg
     3061 gaagccctga ttacagaggc gaggctgatc aagcagtatc agcccttttt caatatcgat
     3121 ctgaagatgg gggagcggta tccttatgta aagatcacca gcgaaccctt tcccagggtc
     3181 gtgatcacaa ggcacaggga agagggggac ggttcagaat atattggacc ctttgtcagt
     3241 gccggcgaac tgcggcatat gctgaggctt gtggagaggt tcttccctat cagggtttgc
     3301 agggcaggat cggggaacgg acaatgggcg aagagaccct gtgtcagata tgagctcgga
     3361 aggtgtccgg gcccctgtgc gggccttgcg gacccttcgg agtacaggga gagggtggcc
     3421 gatatagcgc tgctcctgag gggccggtca gccgatcttg tcggaagact ccgtcagagg
     3481 atggacaagg catcggcctt gatggatttc gagaccgccg caaggatgag agataccata
     3541 agggccctct ggaagctgtc caggaaacat gtctcgatgg tcttttctga accactggac
     3601 ggtggaacct ggaagagcct cgtgcagctt caggatcttc tctcccttcc ggcgatcccc
     3661 tggaggatcg aggggtttga cgtatcccat ttctccggag gtgaggccta cggggtttgt
     3721 gtggtcttcg agcagggaat gcccaacccg tccctgtaca ggagatacag gatcaggaca
     3781 gttgaggggg tagacgactt cagggcaatg ggcgagatcc ttggcagaag gtataccagg
     3841 ctcctgaaag gatcgggggc gttcccccag ctgatcctgg tcgatggagg accggggcag
     3901 ttggaatttg ccgtgaaagc cctcaacacc atcgggatct cggacatacc ggtggtggcc
     3961 atcgccaaga gagaggaact gctcttcact cccggagccg gcggggcgct aaggctcccc
     4021 ctggacagtg gggcgttgat gcttctaaga agggtgaggg acgaatccca ccggttcgcc
     4081 atagcagcca acaggaaagc cgggagaaag aggatgtccc tttcggccct ggaagaggtg
     4141 gccggtgtgg gaaagatcct tgcggcgagg cttctgtcga aattcggaag tgtcgccagg
     4201 atgtccagcc ttacgcccga ggagatagct tcagtaaaag gggtaagcat taaaaccgca
     4261 agaagtataa tcgttcacct tggaggtccg gaggcggaag atgctgacat gtgatccgaa
     4321 agtggacgcc tattacatgg gggcggctct cagccttgcc ctaaggggga cggggagggt
     4381 ctccccaaac cccatggtgg ggtgtgttat cgtggggccc gaaggagttg tggggtgggg
     4441 tttccatgaa ggaccagggg aaccgcatgc cgaggccgtc gcactggacc gggccggaga
     4501 gaaggcacgg ggggcgaccc tttacgtcaa tcttgaacca tgctgccatc aggggaggac
     4561 acctccctgt gccccaagga tcattaaaac aggcatcgcc a
//
LOCUS       DAOPKQ010000105         4505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_21048_length_4505_cov_4.594831, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000105 DAOPKQ010000000
VERSION     DAOPKQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21048_length_4505_cov_4.594831"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PK146_04670"
     CDS             complement(<1..942)
                     /locus_tag="PK146_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91568.1"
                     /translation="MTAVLLLALACGPLPAQEGWELLRDGEPIGKVPVLSRAGHSLVA
                     LDPVMELLGLSSEEKGGNLILKGPGGSLQVFPGAAAAMYRGEIIPFMHEVLREGGHWW
                     VDPDCALTLAGRISGSGSGGLSWRGEGYQPRQTQYPAPGNGPSVQKPQTPVTPLPGPA
                     SASSATVKSIRWGQQDFGLRVVLDLSAPVPLKVEPGSGSVTVTIPGLMSRGTSGTVSP
                     YPSEIGLSVTQFGDRVVLGFRHNASTVRYFSLETPFRQVIDFYDPRPLGTSPVPYSPW
                     TAPPEGTVVEEEPPEEMFQPLSQPVQPPGRTSGGEKTV"
     gene            complement(962..2326)
                     /locus_tag="PK146_04675"
     CDS             complement(962..2326)
                     /locus_tag="PK146_04675"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPE91569.1"
                     /translation="MSHTPSRQEIGSIASWMIETSLRRGARMADVLFYSGSSSEFSLR
                     DGSPERDIRGSSFRIGCRTVDVQGRQGVADTDNLSKDAIGKLIDVCLQNCRASEPEPD
                     VVLYDGEVPDASGLMIFDPGILEVQHPERQERCRQMYELASGTDSRVVSVRDTSWADG
                     SGSTMYASSEGFLGWYDDTYAACSVTVVMSDGGAFEMTGASAESRRIGDVDHSAVARE
                     AVERGTLILGGEAAATGVYDVVLDPEAAASLVESAGELFLASAVFKNRSMLKNRMGDK
                     IGSDCLTLVDDGTIPWGVGTSPWDGEGVPSGRTVLMDHGVVSSFLYNLKYARKFKVAS
                     TGNASRSPGSIPEVGFSNLFVVPGERSPRQILKDCSGGILVTELMGVHTIDPVSGDLS
                     LGVKGALIGPTGQPGKPVAGMTMAGNLVNLLSSISEVGNDLRFFGDIGGCTMVVRDVS
                     MAGT"
     gene            complement(2326..3360)
                     /gene="ltaE"
                     /locus_tag="PK146_04680"
     CDS             complement(2326..3360)
                     /gene="ltaE"
                     /locus_tag="PK146_04680"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HPE91570.1"
                     /translation="MKVIDLRSDTVTKPTDEMRAVMAAAEVGDDVYGEDPTVNRLEEE
                     SARMTGHEAGLFVTSGTQGNLVALLTHCSRGDGAILGSDSHIYIYEGGGLSALGGILP
                     LVAEDSGGIIQPDRVASWCRPSNVHFAPASLLCVENTHNRAGGVAVSPEDFSATVSAA
                     KNGGLLVHLDGARVFNASAAWGVDVKEFTGIVDSVQFCLSKGLGAPVGSMLCGSAEFI
                     SRARHWRKRLGGGLRQAGVLAAAGLIALEKMTGRLSEDHDNAAMLSAMLLAGGVDVES
                     VSKPTNMVYVQLGPDGPDAAELSRRCSARGVLFGAVSERRFRLVTHFGVTKGEVDLAG
                     RTILEELKAS"
     gene            3457..4158
                     /locus_tag="PK146_04685"
     CDS             3457..4158
                     /locus_tag="PK146_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPE91571.1"
                     /translation="MGEAVTRVLVIRAESDGSRVCFDDEVSVEGNYLLVVNGDTLEKV
                     VVSPFALREWVVGHLFCRGSISGAREVKSFEERDGRIEVETDPSSGTEGGPSPAEWTV
                     TPEMIHNGARWIAEAPRFNRTGGFHASALLSATGEKLALFEDISRHNALDKTIGWGLM
                     SEMDLGRTMVIQSGRLPRDMVAKAAAARIPLLGSVSAPTDAGIALARESGITLVGFIR
                     EKRMNIYSFPERIKG"
     gene            complement(4164..>4505)
                     /locus_tag="PK146_04690"
     CDS             complement(4164..>4505)
                     /locus_tag="PK146_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HPE91572.1"
                     /translation="YVASSRLSPGECLRAIQEAGGVSVLGHPGLMGLDEKDLFSLLER
                     LKELGLWGLECISGHHGSGEIYYWMKVASRFGLFPTAGSDFHGIDRPGMDLGVAVNEG
                     LLPWARLGVNI"
BASE COUNT          937 a         1420 c         1287 g          861 t
ORIGIN      
        1 tacggtcttt tctccaccag aagtccttcc agggggttgt accggctggg aaaggggttg
       61 aaacatctct tcggggggct cttcctccac aacggtccct tcggggggag cagtccaggg
      121 ggagtagggg accggcgagg tcccgagggg cctcgggtcg tagaagtcga taacctgccg
      181 gaagggtgtt tccagggaga agtacctcac cgtcgaggcg ttatgcctga agccaagtac
      241 cacacggtcc ccgaactgtg tcaccgaaag cccgatctcc gaagggtagg gagagacagt
      301 accggaggtg cctctggaca taagtccggg tatggtgacc gtcaccgacc ctgaccccgg
      361 ttcgaccttc agggggaccg gggccgaaag gtcaagaaca accctcagtc caaagtcctg
      421 ttgcccccac cggatggact tcactgtcgc cgaggaggct gatgcgggac cggggagagg
      481 ggttaccggc gtctgcggct tctggaccga aggcccattc cccggtgccg gatactgtgt
      541 ctgtcggggc tggtaaccct cccctctcca ggagagtcca ccactgccgg aaccggatat
      601 cctccctgcc agggtgagag cgcaatcagg gtctacccac cagtgtccac cctcacggag
      661 gacctcgtgc atgaacggaa ttatctcacc cctgtacatg gctgccgccg ctccgggaaa
      721 gacctggagc gaacccccgg gacccttaag gatcaggttg ccccccttct cctccgagga
      781 gaggccaagc agttccatga ccgggtccag cgccaccagg ctgtgacccg cccgggaaag
      841 gacggggacc ttgccgatgg gttcgccgtc acggagcagc tcccaccctt cctgcgccgg
      901 gagggggccg caggcaaggg ccaggagcaa gaccgctgtc acaaggacag gggatagtat
      961 cctaggtgcc cgccatagag acatctctta ccaccattgt gcaccccccg atatcgccga
     1021 agaagcgcag atcgttccct acctctgaaa tggatgaaag aaggtttacc aggttcccgg
     1081 ccatggtcat gccagccacg ggctttccgg gttgacctgt cgggcctatc agggccccct
     1141 tgacccccag ggatagatcg ccgcttacgg gatctatcgt gtggaccccc atgagctccg
     1201 ttacaaggat cccaccgctg cagtctttca gtatctgccg ggggcttctc tctccgggaa
     1261 cgacgaaaag gttggaaaac cccacctccg ggatactgcc cggtgacctg gaggcgttgc
     1321 ccgtggaagc caccttgaac ttacgggcat acttcaggtt gtagagaaag gatgacacta
     1381 ccccatggtc catcagtacc gttcttcccg atgggacccc ctccccgtcc cagggagatg
     1441 tccccacccc ccaggggatg gtcccgtcat cgacaagggt aagacagtcg ctgccgatct
     1501 tgtcccccat cctgttcttc agcatggacc ggttcttgaa gaccgccgag gcaaggaaaa
     1561 gctctcccgc ggactccacc agggaggcag cagcctcagg atccagcaca acatcgtaga
     1621 ccccggtcgc ggccgcttct cccccaagga tgagggtgcc tctctcgacc gcctcacggg
     1681 caaccgcgct gtgatccaca tccccgatcc tcctgctctc ggcggaggca ccggtcatct
     1741 cgaaggcacc gccgtcggac atgaccacag tcactgaaca ggcggcgtag gtatcgtcgt
     1801 accatcccag gaaaccctcg cttgaggcgt acatcgtact ccccgaaccg tcagcccagg
     1861 atgtatccct tacggagacg accctgctgt cagtcccgga agccagttcg tacatctgcc
     1921 ggcagcgctc ttgcctttca gggtgctgca cttccagaat cccggggtcg aagatcatca
     1981 agcccgaagc atcgggtacc tcgccgtcat agagcacgac atcgggttcg ggttccgagg
     2041 ccctgcagtt ctggagacac acgtctatca gcttcccgat ggcatccttc gaaaggttat
     2101 ccgtatcggc cactccctgc cgcccttgta cgtccactgt gcggcagcct atcctgaaag
     2161 aagaaccccg aatgtccctt tcaggggatc cgtccctgag ggagaattcc gatgaactcc
     2221 ccgagtagaa gaggacatca gccatcctgg ccccccttcg cagggaggtt tcgatcatcc
     2281 agctggctat ggatcctatc tcctgccggg aaggggtgtg actcatcagg aggccttcaa
     2341 ctcctccagt atcgttcttc cggccaggtc cacctctcct ttcgtaaccc cgaaatgggt
     2401 caccagcctg aaccttctct cggagacagc gccgaagagg acacccctgg ccgagcacct
     2461 ccgtgacagc tcggccgcgt cgggcccgtc ggggcccagt tggacatata ccatgttggt
     2521 aggcttgctc acagattcga cgtcaacgcc tcctgcaagg agcatcgctg acagcatggc
     2581 agcgttatcg tggtcctcgg aaagtcttcc ggtcatcttc tccagtgcta tcagccctgc
     2641 cgcagcaaga accccggcct gtctcagacc tccgccgagc ctttttctcc agtgcctcgc
     2701 ccttgatatg aactcggcgc ttccgcataa cattgagccc acaggtgccc ccaacccctt
     2761 ggagaggcag aactggacgg agtccacgat ccccgtgaac tcctttacgt caacgcccca
     2821 ggccgcggag gcattgaaga ccctggcgcc atccagatgg acaagaagac caccgttttt
     2881 tgcagcgcta acggtggcac tgaaatcctc cggcgatacg gcgacgccgc cggccctgtt
     2941 gtgagtgttc tctacacaga gaagactggc cggagcgaag tgtacgttgc tcggccggca
     3001 ccaggaagcg accctgtcgg gctggattat cccccccgag tcttcggcaa caaggggaag
     3061 gataccgcca agggcggaga gccctccgcc ttcatagata tatatgtggc tgtcggatcc
     3121 gagaatcgcc ccgtcgcccc gggagcagtg cgtcaacagg gcaacgaggt taccctgtgt
     3181 cccggaggta acgaagagac ctgcctcatg acctgtcatc ctggcagatt cctcttcgag
     3241 gcggtttacg gtcgggtcct ccccgtagac atcatcaccc acctctgcgg cagccattac
     3301 tgccctcatt tcatcggtgg gttttgttac agtatcgctt cgaagatcga taactttcat
     3361 taactcagcc ctcctccagc cgtatttcag cagcggcctt atcattgctt gatataatag
     3421 catcattccg ttcctttaaa aaaagggtgg tagacattgg gagaggccgt aacgagagtc
     3481 cttgtcatca gggcggaaag tgatggaagc agggtatgct tcgacgacga agtgtcagtg
     3541 gaagggaact acctgctcgt agtcaacggc gacaccctgg agaaggtggt cgtgtcgccc
     3601 ttcgctctca gggaatgggt cgtgggacat ctcttctgta gggggtcgat ctcaggcgcc
     3661 cgtgaggtaa agagcttcga ggaacgggat gggagaatag aagtggagac cgatccttca
     3721 tcaggaacgg aaggaggacc ttcgccggca gaatggacag tgactccaga gatgatccat
     3781 aacggcgcaa ggtggatagc ggaagcccct cgctttaaca gaaccggcgg gttccacgcc
     3841 tcggcccttc tctccgcaac aggggagaag ctcgcacttt tcgaggatat cagccgccac
     3901 aacgccctgg acaagaccat aggatggggg ctgatgtcgg agatggatct gggccggacc
     3961 atggtcatcc agtcggggag gctgccgagg gacatggtcg ccaaggctgc cgcggccagg
     4021 atcccccttc ttgggagcgt atctgcacca accgatgcgg gtatagccct ggccagagaa
     4081 tcggggatca ccctggtagg tttcatcagg gagaagagga tgaacatcta ttccttccct
     4141 gaaaggatca agggataacc cccctagata ttgaccccca gcctggccca ggggagcaga
     4201 ccctcattaa cggcgactcc caggtccatc cccggacggt cgatgccgtg aaagtcggag
     4261 cccgccgtcg ggaaaagccc gaatcgggag gctaccttca tccagtaata gatctctcct
     4321 gagccgtgat gaccagatat gcactccagg ccccacaggc ccagctcctt cagcctctca
     4381 agaagagaga aaaggtcttt ctcgtccagc cccatcaggc cggggtgtcc caggaccgaa
     4441 actcctcccg cctcctggat cgccctcagg cactctccgg gagagagcct tgagctcgcc
     4501 acgta
//
LOCUS       DAOPKQ010000106         4488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_21170_length_4488_cov_5.206858, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000106 DAOPKQ010000000
VERSION     DAOPKQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21170_length_4488_cov_5.206858"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1181)
                     /locus_tag="PK146_04695"
     CDS             complement(123..1181)
                     /locus_tag="PK146_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPE91573.1"
                     /translation="MQALRTKTRAIMLVVVVVFVISIFAMYITRGSGPSGRPGAEGDL
                     PVATVDGEKVMASHIEMGVRNYAQQSGQSDLSPEWIVQTRKQVLNSIAMQHMLQKEAA
                     RQKITPPREEVDAAVKRIENQFPTKEAFQQYMENSGIKMKDLQEQISMQLSQRMVLDA
                     NAGDIGVTDEEAMEFYDQTRELFFRQPAGYNVMFARFAALEKAEEARKALEGGKKWDE
                     VMQGYEGVALDFTLSEEPAFVTEKEFTEGALKEIAGTPIGKVSKAIALDENNAVIVIK
                     QEKMDEKIASYEEASGDIKGMIAQQKRQTNQNEYLEGLLTKANIQVLVPEFFEVPAQP
                     EAEGEPVPTEGQEEPEGE"
     gene            complement(1291..3822)
                     /locus_tag="PK146_04700"
     CDS             complement(1291..3822)
                     /locus_tag="PK146_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPE91574.1"
                     /translation="MPEKTILLVDGHGLAFRAFFALPELNAPDGTPTNALLGYANMLL
                     KTMEDVRPDLAAVVFDAPGPTFRHEAFEAYKEGRQPTPPEFRVQMPLIKELSRDLGLP
                     VVERPGVEADDVIASTALSAARKGFRVVILTADKDILQVLDENLQVMRPVKGVSEFRL
                     WDLKSFREDYGFSPEAMADYLAMVGDSVDNVPGIKGVGDKTARALLAEHGSLEGVYEH
                     LDGMKPALKKKLEEGRDTVFASRDLTRLRLDEPLQEEDLRLHERKEPELAVLLDRLAL
                     KKLAERLLKGTPAAEAAAAEAPARAELSETSLEPLLEEDGLALGWSGTGDYPQDFSIS
                     EMCLCSDDGRFWKGEADAAVFEKISRWAEKGHVTTIGFKEICAASPSLLREPARVWDV
                     RLAHYALHPEVRVNGAVSASPEETMALWDIRKDLEPQVLSKQLQGVMMDIDTPLCPVL
                     ASMERHGVRVDRDLLTKLGGELDIRTSEISSRIDSMVDTHVNLNSTKQVAWLLFEKLG
                     YPPVKKIKTGFSTDVSVLEELADLPVGDGEVPGMLLEYREITKVITGFIQPLLRQTDP
                     DTGCVHTTFEHTVTGTGRLSSRDPNLQNLPAFGNWAGRLRQALKPRKDGYVFLAGDYS
                     QVELRILAHICGEERLKDAFAQGRDIHSETAYWVFGGDSGVKPEQRRMAKVVNFGLLY
                     GMGVHGLSQRMGIGREEAASVIERYFSAFPRVREYMESSAREARERGYTSTLFGRIRP
                     LSEVSTVEGRGAGAIERVAINTPLQGSAADIARIAMVRYHRAVEEAGLDAPLVLQVHD
                     SLVVECSPGQEEEVSRLLRVSMEGAASLSVPLDVQIKRGATFEDI"
     gene            3918..>4488
                     /locus_tag="PK146_04705"
     CDS             3918..>4488
                     /locus_tag="PK146_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPE91575.1"
                     /translation="MTSTTRVRFAPSPTGALHIGGAHTALFNWLWARRNGGSFVLRIE
                     DTDLVRSTAEFEMTILQGLRWLGLDWDEGPDVDGDHGPYRQSERRDIYLEHAMKLVSS
                     GQAYREGEAVIFSVPRGKMVAFDDVVYGHIEMASDTLKDIVLIKSDGFPTYNYAVVVD
                     DHTMEISHVVRGEDHISNTPKQILLYEALG"
BASE COUNT          835 a         1372 c         1310 g          971 t
ORIGIN      
        1 aagcccccct actcgcttag caggcgagtg ccttcagccg ctcggccatc ccaccctgta
       61 aaattgtgct cggtctattc taggcaccct aaaagggtgt gtcaagaatg cgggaggggg
      121 tgctattccc cctccggctc ttcctggcct tcagtgggga caggttcacc ttccgcctcg
      181 ggctgggccg gaacttcgaa gaactcgggt accaggacct ggatgttggc ctttgtcagc
      241 agcccctcga ggtactcgtt ctggttggtc tgcctcttct gctgggcgat cattcccttg
      301 atgtcgccgc ttgcctcttc gtaggaggcg atcttctcat ccatcttttc ctgcttgatg
      361 actatgacag cattgttctc gtcgagagct attgctttgg aaaccttgcc aatgggggtt
      421 ccggctatct ccttgagggc tccttcggtg aactccttct cggtgacaaa ggcgggctct
      481 tccgaaagag tgaagtcaag ggccactccc tcgtaaccct gcatcacttc gtcccacttt
      541 ttcccgccct ccagggcctt tctcgcctct tcggccttct ctagggccgc gaaacgggcg
      601 aacatgacat tgtaaccggc gggctggcgg aagaagagct ccctggtctg atcgtagaac
      661 tccatggctt cctcgtcggt aaccccaata tcgccggcat tggcatcaag gaccatcctc
      721 tgggacagct gcatgcttat ctgctcctgg aggtccttca tcttgatgcc gctgttttcc
      781 atgtactgct ggaatgcctc cttggtcggg aactggttct ctatacgctt gacggcagcg
      841 tcgacctcct ccctgggagg agttatcttc tgcctggctg cctctttctg gagcatgtgc
      901 tgcatggcaa tgctgttgag gacctgcttt cgcgtctgta ctatccattc gggcgacagg
      961 tccgactgcc ccgactgctg ggcatagttc ctgaccccca tttcaatgtg ggaagccatt
     1021 accttctcgc cgtcaacggt ggcgacgggg aggtccccct ccgcgcccgg cctgccagag
     1081 ggaccgctcc ccctggtgat gtacatcgca aagatggaga tcacgaatac gactacaacc
     1141 accagcatta ttgcccgtgt cttggtcctg agagcctgca ttatcacgat ctatcttcct
     1201 ccttaagcat tggaacggag agtttttcgc cttattttta cagcgtgtac catgatacgt
     1261 tccaacggtg gccctgtcaa aggaaatatc tcatatatcc tcgaaggtgg cgcctctctt
     1321 tatctgcacg tccagaggca cagagaggct agcggctccc tccatggaga ccctcagcag
     1381 tcgggatact tcttcctcct gcccggggct gcactccacc accagtgagt cgtgaacctg
     1441 gagcaccagg ggggcatcca gacctgcctc ttcaacggcc cgatggtaac ggaccatggc
     1501 tatcctcgct atgtcggcgg cgctaccctg gagaggcgtg ttgatagcca cccgctcgat
     1561 ggcgccggcc cccctgccct cgacggtgga gacctccgaa aggggccgga tcctgccaaa
     1621 cagggtcgaa gtgtagcccc gctcgcgagc ctccctggcc gagctctcca tgtattccct
     1681 gacccggggg aaggccgaga agtaccgctc tatcaccgag gctgcctctt cacggcctat
     1741 gcccatcctc tgggagagac cgtgaacgcc catgccgtag agaagaccga agttgacaac
     1801 ctttgccatg cggcgctgct ccggcttcac accgctgtcc cctccgaaga cccagtaggc
     1861 cgtctcggag tggatgtcgc gtccttgagc gaaggcgtcc ttcaggcgtt cctcgccgca
     1921 gatgtgggcc aggatcctga gctccacctg ggagtagtcc ccggcaagaa agacgtagcc
     1981 atccttgcga ggcttcaggg cctgccggag cctgccggcc cagttgccga aggcagggag
     2041 gttctgcaga ttgggatccc tgctgctgag tctccccgta ccggtaacgg tgtgctcgaa
     2101 agtggtgtga acacatccgg tgtcagggtc ggtctggcgc aggagaggct gaatgaagcc
     2161 tgtgatgacc ttggtgatct cgcgatactc caggagcatc cctgggactt ctccatcgcc
     2221 cacagggaga tcggccagct cctccaggac ggaaacgtca gttgagaacc ccgtcttgat
     2281 cttcttcacg gggggatacc ccagtttctc gaagagcagc caggccacct gcttcgtgga
     2341 gttgaggttc acatgggtgt caaccatgga gtcaatgcgg gaggatatct cggaagtgcg
     2401 gatgtccagc tccccgccaa gtttcgtcag aaggtcccgg tcgacacgga cgccgtgacg
     2461 ctccatcgag gccaaaaccg ggcagagagg tgtatctatg tccatcatca ccccctgcag
     2521 ctgttttgag aggacctgcg gttccagatc tttccggatg tcccagagcg ccatggtctc
     2581 ctccggcgag gcgctgacag cgccattgac cctgacctcg gggtggaggg cgtagtgggc
     2641 aagccggacg tcccacaccc gggccggctc cctgagcagt gatggagagg cggcgcagat
     2701 ctccttgaag ccgatggtgg tgacatgccc cttttccgcc cagcgggaga tcttctcaaa
     2761 aacggcagcg tcggcctcgc ccttccagaa acggccgtca tcgctgcaga ggcacatctc
     2821 ggatatggag aagtcctggg ggtaatcccc ggtgccggac catccaaggg caagtccgtc
     2881 ttcttccaga aggggctcca gggaggtctc gctgagttcg gcacgggcag gggcttccgc
     2941 agcagcggct tcagccgcgg gagtgccttt caggagcctt tcagccagtt tcttcagggc
     3001 taatcggtca agcagcacgg ccagttcggg ttccttcctc tcatgaagcc tgaggtcctc
     3061 ctcctggagg ggttcgtcca ggcgaagcct ggtaaggtcc cggctggcga agaccgtatc
     3121 cctgccctct tccagtttct ttttcagggc gggcttcatt ccgtcgaggt gttcgtaaac
     3181 accctccagg gaaccgtgct cggccaggag agccctggcg gtcttgtcgc caacgccctt
     3241 tatcccgggg acgttatcga cgctgtctcc caccatggcc aggtagtcgg ccattgcctc
     3301 gggggagaag ccgtagtctt cccggaagct tttgaggtcc cagaggcgga actcactgac
     3361 gccctttacc gggcgcatca cctggaggtt ttcgtcgagg acctgcagga tatccttgtc
     3421 ggccgtcagt attaccacgc ggaatccctt cctggcggcc gaaagggccg ttgatgctat
     3481 gacgtcgtcg gcctcgacgc cgggcctctc aaccacagga agccccaggt cccgtgagag
     3541 ttcctttatt aagggcatct gcaccctgaa ctccggggga gtaggctgcc ttccttcctt
     3601 gtatgcctcg aaggcctcgt gccggaaggt gggccccgga gcgtcgaaga caacggccgc
     3661 aaggtcgggt ctgacatcct ccatggtctt gaggagcatg ttggcgtaac ccaggagggc
     3721 gttagtgggt gtaccgtcgg gcgcgttgag ttcagggagg gcaaagaagg ctctgaaggc
     3781 aagcccgtgg ccatccacaa gaagtatcgt cttttcgggc aactgcccca tctcctttga
     3841 cagggaattt tggggtatac tgctgttcgg ctcttttaac taccgaatag gataccacca
     3901 gggaaggatg aactgaaatg acttctacca caagggtacg tttcgcaccc agcccaacgg
     3961 gggcgctcca cataggaggg gcccacaccg ccctcttcaa ctggctctgg gccaggcgca
     4021 acggagggtc attcgttctg cgcattgagg acacagacct ggtccgctcc acagcggagt
     4081 tcgaaatgac gatccttcag ggtctccggt ggctcggcct cgactgggac gagggcccag
     4141 acgtggacgg agaccacggt ccctacagac agtcggagag aagggacatc tacctggagc
     4201 acgcaatgaa gctggtaagc tcggggcagg cctaccgtga gggtgaagcg gtgatcttct
     4261 ccgtcccacg gggaaagatg gtggccttcg acgacgtagt ctacggccac atcgagatgg
     4321 ccagcgacac cctcaaggac attgtcctga tcaagagcga cggcttcccc acctataact
     4381 acgccgtcgt tgtcgacgac cacaccatgg agatcagcca cgtggttagg ggagaggacc
     4441 acatctccaa cactccgaag cagatcttgc tctacgaggc tctgggct
//
LOCUS       DAOPKQ010000107         4450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_21406_length_4450_cov_4.536974, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000107 DAOPKQ010000000
VERSION     DAOPKQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21406_length_4450_cov_4.536974"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PK146_04710"
     CDS             complement(<1..1191)
                     /locus_tag="PK146_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPE91576.1"
                     /translation="MSREMRVGVVVFMAIVFLGALVFVSGGARFRQYGYSFGIVFPDA
                     MGLDTGAPILVSGVESGKVDAIELLKEGVLVKVTVRSHVVVPYDSRFTIDTGGLLGEP
                     RVKVVRGASLENISEGDVVSGTIPPALDEIMTDIKTSLGGVQATFSNINSFLAKLSDA
                     AGDFQGFSREARDQVKRAGDSLAGLTDSIDSVVQENRRALSESLDNLSLILENLKAVV
                     DQFDEGGTSGRDLRQMVVKIKEAASSVEELSRKVEETFFDRGGEVPSRFREVREIVGK
                     ANRIISDIEDLTFEGRIGLHGGLSGKFESDPLADISLWIGSRSRRMGFLLGAEDIGGD
                     HGLTAALGLSGEAWRFWGGAVRGYPGAGLVFLPAGVQSPLSIAAQWWNEDGGSWSLEG
                     RYGFN"
     gene            complement(1188..1982)
                     /locus_tag="PK146_04715"
     CDS             complement(1188..1982)
                     /locus_tag="PK146_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE91577.1"
                     /translation="MAASRGEIVDPVMKMQGVSVSFDGKEVISDVEMTLLAGEITCLM
                     GPSGCGKTTTLKVASGLLRPVKGSIELLGKTVGPETDETDLIGIRRELGVVFQGGALF
                     DSMSVGQNIAFPLRYCRGIEDPVSISEMVLDMLDHVELGDVREKYPSELSGGMRKRVA
                     IARSLVHSPSLLLLDEPTTGLDPTTARHIDQLVVELCGKFGFSVLVVTHDITSALGIG
                     DSLLLMHEGRITWRGSPDDWARTDHPVVKKFAEGMVSVGRTGGRKL"
     gene            complement(1963..2742)
                     /locus_tag="PK146_04720"
     CDS             complement(1963..2742)
                     /locus_tag="PK146_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPE91578.1"
                     /translation="MNLLSWIGRQTIELFSTLGFFSIVGLRTFSGVFTGTWSKEDLFE
                     QLERVGVGSVLVVSITSAFTGMVLAVQTLDQFVRFGATGYIGGVIALSMVREMSPVLT
                     GLVVSGRIGAAMAAEIGSMKVTEQIDALRTFGLDPHVFVGVPRLLAGLVMLPILTLFS
                     FVIGIGGGFLYVAAHGVNAHLFRRSVEALLAPSDVYGGLLKAAVFGVLITLSACGQGF
                     MAGQGARGVGTATTKAVVWGNMLILVFNYILSTILFGGIKG"
     gene            complement(2754..>4450)
                     /locus_tag="PK146_04725"
     CDS             complement(2754..>4450)
                     /locus_tag="PK146_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HPE91579.1"
                     /translation="LPSGSLREGAIGVAYTVVKGNEVVSFPVKVLSVMPSARSPRNLI
                     LVRASGPVIENTGGIAAGMSGSPVFIDGKLIGAIGYGWSFSEHQMGLVTPIEDMAEIW
                     NWPDRVPSLNPPELKLEDPSSGDVRLHATVSFDQPGNETEEKEAKELSAPLTGSGYSP
                     RSAKRIEDVIGHPVILLPGGGGDLSLPVEYEPVLRPGEAVSVLVAWGDVSLAATGTLT
                     AISKDGRFLAFAHPFTNRGAVAFPLARAWIHQVVPSLDTPFKIGTPTSIVGIVTQDRP
                     QGIGGFTGRFAPVMDISLNFRDVDSGKETFKRFKTASDPFMMTKVIPEMVTGLVDNVW
                     GRIGEGSAKISLKIEGGKLVDGWRRTNMFFSTTDLAKTMFSEFTDLVNAVSLNPFFEI
                     IPFGVHIDVEATADPRVVLIEDVSLSRKVVPPGGSFDVEITLRPYRKEPVVHKMTLKA
                     PGDAQGVCEVVVRGGGIEEPDQESILMGWRTIRSLDELLKEFSAMETNDEVVAEVRSF
                     GPPREDPSSLEAEEELQRKLRSQRKEEKIREGSFKSYRSNYYVDGLLRRMIRVVPE"
BASE COUNT          999 a         1396 c         1114 g          941 t
ORIGIN      
        1 attgaaaccg taccttccct ccagagacca gcttccccca tcttcgttcc accactgggc
       61 ggcaatggac agggggcttt gaaccccggc ggggaggaag accaggcccg ccccggggta
      121 accgcgcacg gccccccccc agaacctcca tgcctccccc gagagcccca gggcggccgt
      181 cagaccgtga tcaccgccga tatcctcggc ccccaggaga aaccccattc ttctggagcg
      241 acttcctatc cagagcgaga tatctgccaa aggatcgctt tcgaattttc ctgaaagacc
      301 tccatggagg ccgatccttc cctcgaaagt cagatcttcg atatcggaga tgatccggtt
      361 ggcctttccc actatttccc ttacttcccg gaacctggac ggaacttctc cgccccggtc
      421 gaaaaaggtt tcctcgacct tccttgacag ctcctcaacg ctgctggcgg cttccttgat
      481 cttgaccacc atctgccgga ggtcccttcc cgaagttcct ccctcatcga actggtcaac
      541 cacggctttc agattctcaa ggatcaaaga gagattatcc agcgattccg agagggctct
      601 tctgttctcc tggacaacgg aatcgatact gtcggtaagg cccgcaaggg aatcgcctgc
      661 cctcttcacc tggtccctgg cctccctgga aaaaccctgg aaatcccctg cggcatcgga
      721 aagttttgcc aggaaggagt ttatgttgga aaaggtagcc tggacaccgc caagactggt
      781 cttgatgtcg gtcatgatct cgtcgagagc aggcgggata gtacccgata cgacatcccc
      841 ctcggaaatg ttctcaagag aagcaccccg gaccaccttg accctgggtt cgcccaggag
      901 ccctccggtg tctattgtaa aacggctgtc atagggaacg acaacatggc ttcttacagt
      961 aacctttacc aggactccct ccttcaacaa ctcaatagcg tccacctttc cagattcgac
     1021 ccctgagaca aggatgggcg cccctgtatc aaggcccatg gcatcaggaa agacaatgcc
     1081 gaacgagtat ccgtactggc ggaaacgcgc ccctccgctg acaaagacca gggcaccgag
     1141 aaagacaatt gccatgaaga ccacaacacc gactctcatc tctctgctca tagctttctg
     1201 cctcccgtcc tccctacgga taccatccct tcggcgaact ttttaaccac gggatggtcg
     1261 gtcctcgccc aatcatcggg gctccctctc catgtgatcc tgccttcgtg catcagcaga
     1321 agactgtccc cgataccgag agcggaggta atatcgtgtg taactacaag aacactgaat
     1381 ccgaatttcc cgcagagctc cactaccaac tggtcaatat gccgggccgt agtggggtcg
     1441 agaccggtag taggttcgtc aagaagaagt agtgaggggc tgtggacaag ggaccttgcg
     1501 atggcgaccc tctttctcat tcctccggaa agctccgagg ggtacttctc cctcacatcc
     1561 cccagttcaa cgtgatcaag catgtccagg accatctccg agatcgagac cgggtcttct
     1621 atgcccctgc agtacctgag ggggaaagcg atattctgac ccaccgacat ggaatcgaag
     1681 agtgcccccc cctgaaagac aacacccagt tccctccgga tgccaatgag atccgtctcg
     1741 tcggtctcgg gtccgaccgt cttgccgagg agttcaatgc ttcctttcac tggtctcagg
     1801 agacccgaag caaccttcag cgtagttgtc ttgccgcacc ccgagggacc cataaggcag
     1861 gttatctcac cggccaaaag ggtcatttcc acgtcgctta tgacttcctt tccgtcaaat
     1921 gaaaccgaaa ccccctgcat cttcatcacc gggtccacga tctcacccct tgatgccgcc
     1981 aaagagaatg gtggagagga tgtagttaaa tacaagtata agcatatttc cccaaactac
     2041 cgccttcgtc gtcgctgttc cgacgcctct tgctccctga ccagccatga acccttgtcc
     2101 gcaggctgaa agggttatca gtacaccgaa aacggcggct ttaaggagtc cgccgtagac
     2161 atcggaggga gcgagaaggg cctccacgga acgcctgaaa aggtgggcgt tcactccatg
     2221 agctgcaaca tagaggaacc ctccgccgat cccgatgaca aaggagaaga gcgtaagaat
     2281 gggcaacatg acgagacctg ccagaagtct cggaaccccg acgaaaacgt ggggatcgag
     2341 accgaaggtc ctgagggcgt cgatctgctc cgtcaccttc atagatccga tctcggccgc
     2401 catggccgct ccgatccttc cgcttaccac caaccctgta aggaccggag acatctccct
     2461 gaccatgctc agggcgatca ctcctccgat atagccggtg gcaccgaagc gaacgaactg
     2521 gtcaagtgtc tggactgcaa ggaccattcc ggtgaatgcg cttgttattg aaaccaccag
     2581 caccgatccc acgccaaccc tctcgagctg ctcgaaaagg tcctccttgc tccaggttcc
     2641 tgtgaacaca cccgaaaatg tcctcaggcc cacaatggaa aagaaaccca aggtggagaa
     2701 aagctctatg gtctgcctcc cgatccaaga gagaaggttc aaacgtaccc ctcctattca
     2761 gggaccaccc tgatcatcct ccgaagaaga ccgtcaacat aatagttgga acggtagctc
     2821 ttgaacgatc cttccctgat cttttcctct tttctctggc tgcggagttt cctctggagc
     2881 tcctcctcag cctccagaga ggaaggatcc tccctcggcg gcccgaaact ccttacttcg
     2941 gccacgactt catcgttggt ctccatggcc gagaattcct tcagcagttc atcgagactt
     3001 ctgatggtcc tccagcccat gaggatcgac tcctggtccg gttcctcaat accccctcca
     3061 cggacgacca cttcacatac tccctgggcg tcccccggcg ccttgagggt catcttgtgg
     3121 accaccggct cctttctgta ggggcgaagg gttatttcca catcgaaact ccctccgggg
     3181 ggaacgacct tgcgagaaag ggaaacgtct tcaatgagga ccaccctggg gtcggccgtt
     3241 gcctcaacat cgatatgcac cccaaaggga atgatctcga agaaagggtt gagggagaca
     3301 gcgttgacca ggtcggtgaa ctccgagaac attgtctttg ccaggtccgt ggtggagaag
     3361 aacatatttg tcctccgcca tccgtcgacc agcttccctc cctcgatctt caggcttatc
     3421 ttggcgctcc cctcgcctat cctgccccag acattatcca caaggccggt taccatttca
     3481 ggaatgacct tggtcatcat gaaggggtcg ctggcggtct tgaacctctt gaaggtctcc
     3541 ttaccggaat ccacgtcccg gaaattaagg gaaatgtcca tgacgggtgc gaaccgacct
     3601 gtaaaaccac ctataccctg aggacgatcc tgggtcacta tccccactat ggaggtcggt
     3661 gttccgatct tgaagggcgt atccaggctt ggcacaacct ggtgtatcca ggcccttgcc
     3721 agggggaagg ccacagcccc ccggttggtg aacgggtgtg cgaaggcgag gaagcggccg
     3781 tccttcgata tcgctgtaag ggtacccgta gcagcaaggg agacatcccc ccaggctaca
     3841 agaacactta cggcctcacc gggtctgagg acaggttcgt actcgaccgg aagggacagg
     3901 tcacctcctc ccccggggag gaggatcacc ggatgtccga tcacgtcctc gatccttttc
     3961 gcactcctgg ggctgtaacc gctgccggtc agaggagctg aaagctcttt cgcttccttt
     4021 tcttctgtct cattaccagg ctggtcaaat gaaaccgttg catgtagtct cacatcccca
     4081 gaggaaggat cttcgagctt cagctccgga gggttcaagg acgggaccct gtcgggccag
     4141 ttccatatct ctgccatatc ctctattggg gtcaccagcc ccatctggtg ttcgctgaaa
     4201 ctccacccgt agccgatagc ccctatcagc ttgccgtcga tgaagacagg gctaccgctc
     4261 attcctgcag ctatgccacc ggtattttct atcacgggac ctgaagccct gaccaggatc
     4321 aggttgcggg ggctcctggc agagggcatg acgctgagca ccttaacagg gaaagaaact
     4381 acctcgttac ccttgacgac agtgtaggca actcctatcg ccccctccct gagtgatcct
     4441 gatggaaggg
//
LOCUS       DAOPKQ010000108         4443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_21443_length_4443_cov_4.750000, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000108 DAOPKQ010000000
VERSION     DAOPKQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21443_length_4443_cov_4.750000"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /locus_tag="PK146_04730"
     CDS             <1..1888
                     /locus_tag="PK146_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPE91580.1"
                     /translation="GICRQCYGMDLSSREKVLPGETVGVVAAQSIGEPGTQLTMRTFH
                     TGGVRITGEDITQGLPRIEQLFEVRRPKKVAFLSDRDGYISEIREMEGKRKVFISSSE
                     EGVESRSSYNIPASQPLLVEEGQQVRKGQPLTDGHVDPQQLLEIEGLEAVQNYLVDNI
                     QSVYLSQGVSVSNKHIEVILRKVAPVNRVRVIEEGDSSFVSMELAWLSDLEREVENIK
                     AGNERFLLEAVKIMSGKKLLDVADQGSLESALVFKGQELDERSVRQILRPGSGVSELT
                     LEDEGDILRVIVGESSFRRTMSGLELTETAELTEEETIPSGTALSGSSLTQLTQGFPP
                     RILVRDVAILEEMKGKTYLAEDVVVGDNKLLDADRVIDDHAASVIRENNVEAIRIWKS
                     PETVTISDSVQKMLIDQYWARPLVQAIDSDGNSVTDIPQLIDGSVVRGLVEGELTAVE
                     IDGEILSRDAILRKVLSDVAYGKVLLEPVINKTGEVVADAGSEVNTQVLEALLSADPD
                     EMVLRPIFTQAETKRTLQRVTFVRRLREEPIWKPVIHGITKAALATDSFLSAASFQQT
                     AQVLAGAAVRGDVDKLKGLKENVIIGHLIPAGTSFEMYKDIRVKETPSSVEEPPREEV
                     ILNP"
     gene            1973..2266
                     /locus_tag="PK146_04735"
     CDS             1973..2266
                     /locus_tag="PK146_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPE91581.1"
                     /translation="MPLNELAVPNRLVGTRSVRKAIQAGVLRKIFLAADAKGSLVKDL
                     ERMARESGIKVEWAESMVILGRACAIDRGAAAAGLTIRKGPPITGAEDLKSNE"
     gene            2282..2653
                     /gene="rpsL"
                     /locus_tag="PK146_04740"
     CDS             2282..2653
                     /gene="rpsL"
                     /locus_tag="PK146_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPE91582.1"
                     /translation="MPTINQLIRKGREEKRVRSKAPALQGNPARRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNGFEVTSYIPGVGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DCGGVDGRRKSRSKYGARRPK"
     gene            2696..3166
                     /gene="rpsG"
                     /locus_tag="PK146_04745"
     CDS             2696..3166
                     /gene="rpsG"
                     /locus_tag="PK146_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPE91583.1"
                     /translation="MPRKGYVRIREIEADPRFGNLDIAKFINAIMLDGKKSVAEKIMY
                     GALDLASKRLSMEPIEVFNKAMENVRPMVEVRPRRVGGATYQVPVEVQPRRAQALATR
                     WILQYARGRKGIPMMERLAREFTDACKGEGSSVKKREDTHRMAEANRAFAHYRW"
     gene            3239..>4443
                     /locus_tag="PK146_04750"
     CDS             3239..>4443
                     /locus_tag="PK146_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPE91584.1"
                     /translation="MSSIDLGKIRNIGIAAHIDAGKTTTTERILFYTGRKHKLGEVHE
                     GAATMDWMEQERERGITITSAATTCHWKGYSVNIIDTPGHVDFTVEVERSMRVLDGAV
                     AVFCAVGGVEPQSETVWRQADRYRVPRVAFVNKMDRVGADFHLVVSQMKERLGANAVP
                     VQIPIGSEDGFSGVVDLVREKAIIYHEELGVDPKVVDIPAELAEDAAMGRDALVEAMA
                     DLDEDVMTLYLEGKPVPEDILRKAIRKATIELAVFPVLCGSAFKNKGVQALLDSVVDY
                     LPSPVDLPPIKGVHPSTGNTEERHPDPDGPLSALAFKIVVDPFVGRLVYCRIYSGRIK
                     SGSSILNTSTEGRERVGRILRMHANKREDIEEASAGEIIALPGLKATRTGDTLTDEKK
                     PVVLESVDFP"
BASE COUNT         1012 a         1204 c         1428 g          799 t
ORIGIN      
        1 tggaatatgc aggcagtgct acggcatgga cctctcttcg agggagaagg tactccccgg
       61 agagaccgtg ggggtcgtgg cggcccagtc catcggggaa ccggggacgc agctaaccat
      121 gcggaccttc cacaccggcg gtgtccgtat caccggggag gacattaccc agggtctccc
      181 caggatcgaa cagctcttcg aggtccgtcg gcccaagaag gtggcctttc tctccgaccg
      241 cgacggctac atatcggaga taagggagat ggagggcaag aggaaggttt tcatatcctc
      301 cagcgaagag ggtgtcgagt ccaggtcctc atacaacata cctgcgtccc agccgctcct
      361 tgttgaggaa gggcagcagg tcaggaaggg gcagcccctt accgacggtc atgtcgatcc
      421 ccagcagctc ctcgagatcg agggacttga ggcggtccag aactacctgg tggacaacat
      481 ccagtccgtc tacctgtccc agggggtctc ggtcagcaac aagcacatcg aggtcatcct
      541 ccgcaaggtc gctcccgtga acagggtgag ggtgatcgag gagggggaca gctccttcgt
      601 gtccatggag cttgcgtggc taagcgacct ggagagggag gtcgaaaata tcaaagccgg
      661 caacgagcgc ttcctcctgg aagcagtgaa gataatgtcc ggcaagaagc tcctcgacgt
      721 cgccgatcag ggcagtctgg agtctgcgct ggttttcaag ggccaggagc ttgacgaaag
      781 gtcggtccgg cagatactcc gccccggttc gggcgtatcg gagcttaccc tcgaggatga
      841 gggcgatatc ctccgcgtga tagtgggcga atcatccttc aggaggacca tgagcgggct
      901 ggagctcaca gagacggctg agctcacaga agaggagacc atcccgtccg ggactgccct
      961 gtcaggttcc agcctgacgc agctcaccca gggcttcccg ccgagaatac tcgtcaggga
     1021 cgtggccatc ctggaagaga tgaaaggtaa gacctacctt gcagaggacg tcgtggtggg
     1081 agataacaag ctgctggatg ctgacagggt catcgacgac catgccgcct cggtcataag
     1141 ggagaacaac gttgaagcca taaggatctg gaagtctccg gagacggtca ccatctccga
     1201 ctccgttcag aagatgctca tagaccagta ctgggcaagg cccctggtgc aggctataga
     1261 ctccgacggg aactccgtca ccgacattcc ccagcttatt gacggtagtg tagtccgggg
     1321 actcgtcgag ggcgaactca ccgctgtgga gatcgacggc gagatcctct ccagggatgc
     1381 tattctgagg aaggtgctct ccgatgtcgc ttacgggaag gtccttcttg aacccgtcat
     1441 caacaagacc ggcgaggtag tggccgacgc cggcagcgag gtcaataccc aggtgctgga
     1501 agcgctgctg tcagcagatc ccgacgagat ggtcctcagg cccatattca cccaggccga
     1561 gacgaaacgg accctccagc gggtgacctt tgtccgaagg cttcgcgagg agccgatatg
     1621 gaagcctgtc attcacggga tcaccaaggc cgccctggcc accgacagct tcctcagcgc
     1681 ggcctcgttc cagcagaccg cccaggttct tgccggtgcc gctgtcaggg gagacgtgga
     1741 caaactcaag gggctcaagg agaacgttat catagggcac cttatccctg cggggacttc
     1801 cttcgaaatg tacaaggata taagggtcaa ggagacgcca tcttccgtgg aggaacctcc
     1861 ccgggaggag gtcatcctga acccatgaca tggggaagat aagatttttc ttgacagtga
     1921 ctggtgccgt tgatattatt tcccggtgtc cttttcaagg gagggattac gtgtgccctt
     1981 gaacgagttg gctgtgccca accggctggt aggcacaaga tcggtcagga aggcgatcca
     2041 ggcaggggtt ctcaggaaga tcttccttgc ggcggatgca aaaggatccc tggtgaaaga
     2101 ccttgaaagg atggccagag agtccggcat aaaggtcgag tgggccgaaa gcatggtgat
     2161 actgggccgg gcctgcgcca tcgaccgggg tgcagcggcg gcgggattaa cgatacgaaa
     2221 gggtcccccg atcaccgggg cagaggatct aaagagcaat gaataagaag gagggagttt
     2281 tttgccgacc atcaatcagc tgatacgcaa ggggcgtgaa gagaagaggg tgcgctccaa
     2341 ggcgcctgct ctccagggca atcctgcaag gagaggcgtc tgcaccaggg tctataccgt
     2401 aacacccaaa aagcctaatt cggccctcag gaaggtcgca agggtccgtc tgaccaacgg
     2461 tttcgaggtc acttcctaca ttcccggagt ggggcacaac ctgcaggagc actctgttgt
     2521 gctggtaagg ggcggccgtg tcaaggacct tccgggagtc cgttatcaca tcgtcagggg
     2581 cacccttgac tgcggcggtg tcgacggcag acggaagagc cgttccaagt acggcgccag
     2641 acggccgaag taggaccaat agacaggctt tattagcagg gagagtgtcg aggacatgcc
     2701 aagaaaggga tacgtgcgca ttcgcgagat cgaggccgac cccaggttcg ggaaccttga
     2761 tatagccaaa ttcatcaacg ccatcatgct tgacggcaag aagagcgtcg ccgagaagat
     2821 catgtacggt gctctggacc tcgcctccaa gaggctcagc atggagccca tagaggtctt
     2881 caacaaggcc atggagaatg tccgtcccat ggtcgaggtc cgccccagaa gggtcggcgg
     2941 ggctacctat caggtccccg tagaggtcca gcccaggagg gcccaggctc ttgcaaccag
     3001 gtggatcctc cagtatgcca gggggaggaa gggcatcccc atgatggaga ggctggccag
     3061 ggagtttacc gatgcatgca agggcgaagg gagttccgtc aagaagcgcg aagataccca
     3121 caggatggcc gaggccaaca gggcctttgc gcattaccgc tggtagaaag tcttaggccc
     3181 attctgccgg gtgcgggtca acccgcaacg gcagcgggcc gggtatggag aaaactgaat
     3241 gagctctatc gatctgggga aaatcaggaa catcggtatt gcggcgcaca ttgacgccgg
     3301 caagaccacc acaacggaga ggatcctgtt ctacacgggc cggaagcaca agctcggaga
     3361 ggtccatgaa ggcgctgcca caatggactg gatggagcag gagagggagc gggggataac
     3421 aattacctcc gctgctacga cgtgccattg gaagggttat agcgtcaata ttattgatac
     3481 gcccggccac gtggatttta ccgtagaggt cgaaaggtcc atgagggtcc tcgacggagc
     3541 ggtggctgtc ttctgtgccg tcgggggagt cgagccccag tccgagacag tatggaggca
     3601 ggccgacagg tacagggtgc ccagggtcgc cttcgtgaac aagatggaca gggtcggtgc
     3661 ggatttccat ctggtggttt cccagatgaa ggagcgcctg ggggcgaacg cggtccccgt
     3721 tcagatccct ataggctccg aggacggttt cagcggtgtc gtcgacctgg tgagggagaa
     3781 agcgataatc taccacgagg aactgggtgt cgacccgaag gttgtggata tccccgccga
     3841 actggcagag gacgcggcca tgggacggga tgccctcgtg gaggcaatgg cggaccttga
     3901 cgaagatgta atgaccctct acctggaggg caagcccgta cccgaggata tcctcaggaa
     3961 ggccatcagg aaggccacga tcgaactggc cgtatttccc gtcctctgcg ggtcggcctt
     4021 caagaacaag ggggtccagg cactgctcga ctcggttgtg gactatcttc ccagcccggt
     4081 ggacctccct ccgatcaagg gcgtacaccc ctcgacaggg aataccgagg agaggcatcc
     4141 tgaccccgat ggtccactct cggcactggc cttcaagatt gtggtcgacc ccttcgtcgg
     4201 acgtcttgtc tactgcagga tctattcggg gaggatcaag agcggttcaa gcatcctgaa
     4261 cacctccacg gagggccgcg agagggtagg caggatactc aggatgcacg ccaacaagag
     4321 agaggacatc gaggaggctt ccgcgggcga gataattgcc cttccgggcc tgaaggccac
     4381 caggacaggg gataccctga ccgacgagaa gaaacccgtt gtccttgagt cggtggattt
     4441 ccc
//
LOCUS       DAOPKQ010000109         4390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_21785_length_4390_cov_4.598155, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000109 DAOPKQ010000000
VERSION     DAOPKQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21785_length_4390_cov_4.598155"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..735
                     /locus_tag="PK146_04755"
     CDS             187..735
                     /locus_tag="PK146_04755"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HPE91585.1"
                     /translation="MPDRRCFPYLSGPGPHSLILGSFPGERSLREGRYYAHPRNAFWS
                     IMGRLFNAGPELPYEERLERLSSAGIALWDVLLTCRRTGSMDGSIIEDGAIPNDLPGF
                     FLEHPTITAIFFNGQKAARLFERLAAPRLGEISLRMEWKVLPSTSPAYASKSLEQKVE
                     EWRVLPGAGRPYLDRKSSLRSF"
     gene            complement(699..1262)
                     /locus_tag="PK146_04760"
                     /pseudo
     CDS             complement(699..1262)
                     /locus_tag="PK146_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            complement(1278..1433)
                     /locus_tag="PK146_04765"
     CDS             complement(1278..1433)
                     /locus_tag="PK146_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91586.1"
                     /translation="MKILRVFVLMAVLVAFMKVGEASMEGKGSPVLPPIDLSQADRFE
                     TATFAMG"
     gene            complement(1521..2075)
                     /locus_tag="PK146_04770"
     CDS             complement(1521..2075)
                     /locus_tag="PK146_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPE91587.1"
                     /translation="MSVRNAMTAENLRAAYGGESMAHMRYLVWGEAAEKEGFPKIAKL
                     FRAVSHSEWVHARNHFTVLDGEIGDHPVTAGGVFGLKSTADNLTGGFMGEEFEIDQMY
                     PVYLKVAEFQEEKGAVRTFKWALEAEKIHAELFHRGHEAARSGKDVDYDSFFICTVCG
                     HTAPEKAPDFCPVCGAKKEAYKEF"
     gene            2357..3628
                     /locus_tag="PK146_04775"
     CDS             2357..3628
                     /locus_tag="PK146_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE91588.1"
                     /translation="MKKSLFSKGLLLVLTAFLVVGAISGAAVAEEKVIKIGTLFPLTG
                     PVALAGQRCRAAVETAAEVINNAHPELQVPLAAQEGVLDGYKFVLVHADHQGKPDVAK
                     SEAERLFDQEKVWGIIGCYNSAATKPASFVAELKQRVFMCGCSSSGALTERDFKYFFR
                     MAPTDKTESLEFVEFIEWANEHKGSDIKTVGLIYENTEFGKHAAEEGRMAAEAGGFKV
                     VADVAFNPGATNLNSEVQTLKAANPDAVFGACLGGDYTLWVRTMKQMDWLPKVALNYC
                     TGYQDPIIVGQLGDDANYFMGGTGYAPEFAELMPAVAAVEEIYKTKTDPSVPFDSDSI
                     QEAVALLVLAQAIEKSGSLDPTVVAETLHANEWESPLSMGGKVAFAPGGQNIKAMSML
                     TQLQGGEYKRIYPVDLADTEPVIPMVPWKDR"
     gene            3797..>4390
                     /locus_tag="PK146_04780"
     CDS             3797..>4390
                     /locus_tag="PK146_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE91589.1"
                     /translation="MATFLQVAFDGLLNGLLYALVAAGLSLIWGVMDVINFAHGEFLM
                     IAMYFSYWLGFIWKIDPIISSVAGGAFVFIVGALTYRLVIRRTIGKSAMSALLATFGL
                     SMLIKNICLNRFSPNFRLLQDTLLGGKTFNLAGIIIPVPQLVIGLVALALLFVVYRLV
                     KKTRLGWAIQATAMDKEAAELMGINTERIYLLVFGLGG"
BASE COUNT          935 a         1273 c         1215 g          967 t
ORIGIN      
        1 ctagaatgct tagctgtttt cctgcagggc tcagcgtacc ccaaagaaca cccgcggcaa
       61 ggacggcaag gaaatcggtc cggatgcgtt ccctcaaggc ggcagcaacc tctcctgtgg
      121 acttgtgacg gtttttgcgt tttcattgta tcatccatcc tgtggagcaa cggaggtgat
      181 gatcggctgc cggacaggag atgtttcccc tatttatccg gaccgggccc gcattctctg
      241 atactgggaa gtttccctgg cgaaaggtct cttcgggagg gacgctacta cgcccacccg
      301 aggaacgcct tctggtccat catgggcagg cttttcaatg cgggtccgga acttccctac
      361 gaagagcggc ttgaaaggct cagctctgcc gggatcgccc tttgggacgt tctgctgaca
      421 tgccggagga ccggcagcat ggacggttcc atcattgagg acggggccat accgaacgat
      481 cttccgggtt ttttcctgga acacccgaca atcacggcga tattctttaa cggccagaag
      541 gcggcccgcc tgttcgaacg gcttgccgcc ccgcgccttg gagagatatc gctgaggatg
      601 gaatggaagg tccttccgtc aacaagtccg gcctacgcct cgaaaagtct ggagcaaaag
      661 gtcgaagaat ggagagtcct gccgggggct ggtcggccct atcttgacag aaaaagctct
      721 ctcagaagtt tctgaccctc cggggagagt ccgagcatgg gcagctcccg gtcgagctgt
      781 tcaagagtcc cgtaacctcc gagataaccg ttggccctgg ccgcggccgt cgaagcaaca
      841 aggtcctctt caagggggaa caacaccctg aattcatcgt agatcgccgg agttcccttc
      901 aaggcgaatt tctggtggta atcctcggcc gggtagaacc tctccagctt cctgatctca
      961 gtcctgacct tcctgccgct cttcttctcc agttcggcgg cctgctcaag agcagtttgc
     1021 tcctgatccg gatcgtggaa gagaaggagc gaggcgtact gccttctgaa agacatggca
     1081 aaggggtcgt gccccttcca gaactccgca agaagttccc cgtatgagac tacaaggggg
     1141 tcgaaatcaa cctgaaggct ctcggtatgg tcccccaggt tgtagtaacc ggggtcgggc
     1201 ttatcgccgc cggcgtaacc cacccttgtc cttatgaccc catcgatgct cccgaaccgg
     1261 gagtcgggac cccagaatca gcccatggcg aacgtggctg tctcgaaccg gtccgcctgt
     1321 gacaggtcaa taggaggaag aacagggctt cccttccctt ccatgcttgc ttcaccgact
     1381 ttcatgaaag cgacaaggac agccatgagc acaaacaccc ttaagatctt cactccctgt
     1441 cgccccctaa aatgaaaata ttttatttac gcaatataca gcctgggact gaaggggtca
     1501 acagtcgggg caagaccgga tcagaactcc ttgtaggcct cctttttggc accgcaaacg
     1561 gggcagaaat ccggagcctt ctccggggct gtgtggccgc agacggtgca gatgaaaaag
     1621 gaatcatagt ccacgtcctt gcccgacctg gcagcctcat gccccctgtg gaagagctcg
     1681 gcgtggatct tctccgcttc aagggcccat ttaaaggtcc ggacggctcc cttttcctcc
     1741 tggaactccg ccactttcag gtagaccggg tacatctggt ctatctcgaa ctcctcgccc
     1801 atgaagcccc cggttagatt atcggcggta ctcttgagcc cgaatactcc cccggcagtg
     1861 acaggatgat cccctatctc accgtcgagg acggtaaagt ggttcctggc gtgcacccat
     1921 tcggagtggg aaactgccct gaaaagcttt gcgatcttcg ggaaaccctc tttttcggcg
     1981 gcctcacccc aaaccaggta acgcatatgg gccatgctct cgccgccgta ggcggctctc
     2041 aggttttctg ccgtcatggc atttcttaca gacataggaa cactcctccc tggtattttg
     2101 atcttttctc ccctgcgtgg gatcgcgcgg agcccatttc tactctcagg gctctgatcg
     2161 gcgaacagga aactcctcct ggtaccccgg ataattattg caaaaaacat gaccttttgc
     2221 taacagggtt cacagggacc gggaagcgat ttcttgacca ataagtcccc tttcctctaa
     2281 aatcaaagtc ctggagaatt aaagaacaaa gtggcccaca ggccgctaga acactattga
     2341 gaaggaggag tacagaatga agaagagtct tttcagtaag gggttgcttc ttgttctgac
     2401 ggcctttctg gtcgtgggag cgatctctgg agccgccgtt gccgaggaaa aggtcatcaa
     2461 gataggaacc cttttcccgc tgacgggacc cgttgccctt gccggtcaac gatgcagggc
     2521 agctgtcgag accgcggccg aggttatcaa caatgcccac cccgagctgc aggtacccct
     2581 tgctgcccag gaaggcgtac tggacggcta caagttcgtc cttgtgcacg cagaccacca
     2641 gggcaagcct gacgtggcca agtccgaggc cgagcgcctt ttcgaccagg agaaggtctg
     2701 ggggatcatc ggatgctaca acagcgccgc gacgaaaccg gccagtttcg tggccgagct
     2761 caagcagaga gtcttcatgt gcggatgttc aagctcaggt gccctgaccg agcgcgactt
     2821 caaatacttc ttccgcatgg cccccaccga caagacggag tctctggagt tcgtggagtt
     2881 catcgagtgg gccaacgaac acaagggctc tgacatcaag accgtcggac tcatttacga
     2941 gaacaccgag ttcggaaagc acgctgctga agagggccgt atggctgccg aggctggcgg
     3001 tttcaaggtc gtcgccgacg tagccttcaa ccccggcgcg accaacctca acagcgaggt
     3061 ccagacactg aaggccgcca accccgatgc cgttttcggc gcctgcctgg gtggcgacta
     3121 caccctctgg gtccgcacca tgaagcagat ggactggctt ccgaaggtcg cactcaacta
     3181 ctgcaccggc taccaggatc ccatcatcgt cggccagctg ggggatgacg caaactattt
     3241 catgggcggc accggttacg cccctgagtt cgctgaactg atgcccgccg tcgcagctgt
     3301 cgaggagatc tacaagacca agaccgatcc cagcgtcccc tttgacagcg actccatcca
     3361 ggaagcagtt gcgcttttgg tcctggccca ggctatcgag aaatccggaa gccttgaccc
     3421 gacggtggtc gccgagaccc tccacgccaa cgaatgggaa tcgcctctct ccatgggcgg
     3481 caaggtggcc tttgcccccg gcggacagaa catcaaggca atgagcatgc tgacccagct
     3541 tcagggcggg gagtacaaga ggatctaccc ggttgacctt gccgacacgg aacctgtcat
     3601 acccatggtc ccctggaagg atcgctgatc caggcctgaa ctcctgtatc tcgggtaagt
     3661 tactggaccc gcttctttgc aggactgatc gcggatctac cagagaggca gcaggggttt
     3721 ctctgttgcc tctctttttt cgcaggtctg cggttttttg aaatgaaaag aatgatgaac
     3781 tgggggtgta ccctgaatgg ccacgtttct ccaagtcgca tttgacggac tgctgaacgg
     3841 gctgctttac gcactggtag cggcgggtct tagcctgata tggggcgtca tggatgttat
     3901 caactttgcc catggcgaat tccttatgat cgcgatgtac ttcagctact ggcttggctt
     3961 tatctggaag atcgatccca ttatctcttc cgttgcgggt ggagccttcg ttttcatagt
     4021 aggggccctg acatacaggc ttgttatcag gaggaccatc ggtaagtcag ccatgtcagc
     4081 tcttcttgct accttcgggc tttccatgct tatcaagaac atatgtctga accgtttctc
     4141 gcccaacttc cggcttctgc aggacacgct tctgggtgga aagaccttta acctggcggg
     4201 gataataatt ccggtccccc agcttgtcat cgggctcgtc gcgctggctc ttctgttcgt
     4261 cgtctaccgg cttgtcaaaa agacccgact ggggtgggcg atacaggcaa cggccatgga
     4321 caaggaagcc gcggaactga tggggatcaa taccgaaagg atctacctgc tagtctttgg
     4381 gcttggtgga
//
LOCUS       DAOPKQ010000110         4341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_22129_length_4341_cov_5.689221, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000110 DAOPKQ010000000
VERSION     DAOPKQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22129_length_4341_cov_5.689221"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /gene="dctP"
                     /locus_tag="PK146_04785"
     CDS             complement(<1..673)
                     /gene="dctP"
                     /locus_tag="PK146_04785"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPE91590.1"
                     /translation="MREVNCPDFAGQDIKMRKLLTGILVLALLFGLASVDAASAALKG
                     EYKLTMNVTTDTAWGQGGVKFAELVKEYTGGKVNVKVYPSAQLVGGDQMRQAEMVSSG
                     AIDFMLNSTINIAPIIPECNAFSLPFMFPSYESVDAVTGSAAGQMVFDALEKHNMVGL
                     AWGENGFRELTNNVKPIAHPDDMKGLKIRVVTPIYIDIMRALGANAIAMNWGEVFTSL
                     QQGVID"
     gene            complement(773..1405)
                     /locus_tag="PK146_04790"
     CDS             complement(773..1405)
                     /locus_tag="PK146_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE91591.1"
                     /translation="MRERTKTDSVYEEIKKLILRGELSSDTPISENYLAQRFGISRTP
                     IREALKRLDMEGFVRIIPNQGVFVRDVSISEVKEIYDLRIALEEFVVKELTHTLSEDD
                     LFVLEGNLARQEEALRRHDAFAFHEEDRKFHDYFMKAYGNDMITDFITNLRDRIEGIN
                     VNMLKQPGNMELFWGEHRRILEALRRRDGEGATREMDEHLKGGKERLLRG"
     gene            1596..2573
                     /locus_tag="PK146_04795"
     CDS             1596..2573
                     /locus_tag="PK146_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPE91592.1"
                     /translation="MTVKILDLKITPGVSVSLIDLPLDLDGYHDFFGIWLVRDEGRER
                     NTLVDVGPTSTASILIEELHTLDVKKLDYILLTHIHLDHSGGLAELLRAFPSARVAVH
                     PKGKRHLVNPERLWESSLQVIPEMALAYGEPSPVDESCFIPEEVPVPGITAVDTPGHA
                     PHHRSFFYWTPEGQVLFAGEAASTFTRLDHLVPGAGERRYMLRPASPPRFYIETALRS
                     IEELKGIDASLLCYAHFGCTEQVREMLDHAGEQIRLWRKLFSEFLNMKGYKFENQVEM
                     GELLGFVVERDPWLADFSLLPADVRSREMRFMLSSAAGFLGAVLEERKV"
     gene            complement(2580..3035)
                     /locus_tag="PK146_04800"
     CDS             complement(2580..3035)
                     /locus_tag="PK146_04800"
                     /inference="COORDINATES: protein motif:HMM:NF023374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HPE91593.1"
                     /translation="MRSIRRRSLVILAVLAALVLVFAERGFLGPSAIRVPEQVSAGAE
                     LLESLYRERKGGVQAEAGGIVTRLLPDDDRGSRHQRFVVELPTGQTLLIAHNIDLAPR
                     IDGLRTGDRIEFSGEYEWNPEGGVIHWTHRDPSGKHRPGWLVHEGQKYW"
     gene            complement(3337..>4341)
                     /locus_tag="PK146_04805"
     CDS             complement(3337..>4341)
                     /locus_tag="PK146_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPE91594.1"
                     /translation="PAERWRDEPHRLKDPDKLIETVRLIAPSFGGINLEDIAQPKCFH
                     VLDELRKDCKIPVWHDDQQGTAAVNLAGLYSGLKITGKKIDEAKVRFFGAGAASIATA
                     RILISAGVRPENVIMADSKGTLHKGRTELKDVPYDVKWHFAQTTNPEGIEGGPEVLFR
                     DADVVYCYTKPGPDVVKKEWVATMAKDAMLFACANPIPEIWPWDAKEAGARIVATGRS
                     DFPNQINNSLGFPGIFRGTLDVQASTITDEMCIAAAKTLSKCAEKNGLSEDYLIPTMD
                     QAWVYPEVATAVGMKAIEQGIARKVLSGEELLKTATEKIQYAQKMTACLTEKKFIELP
                     "
BASE COUNT          868 a         1273 c         1144 g         1056 t
ORIGIN      
        1 cgtcgataac gccctgctgc agcgaggtaa atacctcgcc ccagttcatg gcgatggcgt
       61 tcgcgccgag ggctctcatg atatcgatgt atataggtgt tacgaccctg atcttgagac
      121 ccttcatgtc atcggggtga gctatgggct tgacattgtt ggtgagctca cggaaaccgt
      181 tctcgcccca tgccagtccg accatgttgt gcttctcaag ggcgtcgaag accatctggc
      241 ccgctgccga gccggtcaca gcatcaacgg actcgtaact ggggaacatg aaggggagcg
      301 aaaaggcgtt gcattccggg atgatcgggg cgatgttgat cgtggagttg agcatgaagt
      361 ctatagcacc actggagacc atttcagcct ggcgcatctg gtcgccgccg acaagctggg
      421 cgctggggta gacctttacg ttcaccttgc ctccggtgta ctccttgacc agttcggcga
      481 acttgacccc gccctggccc caggcagtat cggtggttac gttcatagtg agcttgtact
      541 cacccttgag tgcggctgaa gctgcatcaa cgcttgccaa accgaagagc aaagccaata
      601 ccagtattcc tgtcagcaat ttcctcatct taatgtcctg tccggcgaaa tccggacagt
      661 tcacctccct cacaggtttt tttacctccc agttggggaa acctctcctc tgcccctgtc
      721 gttatccctc ctctcaggat aataaacggt cactgccgta cctctctacg gatcagcccc
      781 tgagaagtct ctccttgccg cccttcaggt gctcatccat ctctctggtt gccccctctc
      841 cgtcccttct gcggagggct tccagtatcc ttctgtgctc accccaaaaa agttccatgt
      901 tccctggctg tttcagcatg ttcacgttga tcccctcgat acggtcacgc aggttcgtta
      961 tgaagtcagt gatcatgtca ttaccgtagg ccttcatgaa gtagtcgtgg aacttacggt
     1021 cctcttcatg gaaagcaaag gcatcgtgtc tacggagggc ttcttcctgc cgggcaaggt
     1081 tcccctcgag cacaaagaga tcatcttcag acagggtgtg ggtaagttcc tttacaacaa
     1141 actcttccag ggctatccgc aggtcatata tttccttgac ctcgctgatc gaaacgtcgc
     1201 ggacaaagac accctggttg ggtatgatcc tgacgaaacc ctccatatca agacgcttca
     1261 gagcctcacg gataggcgtc cggcttattc cgaacctctg ggcgaggtag ttctccgaaa
     1321 tcggtgtgtc actggatagc tcccccctga ggatcaactt ctttatctcc tcgtaaaccg
     1381 aatcggtctt cgttctctct ctcaaggatc gcatcccctt actggtatac aaccattatt
     1441 caactgaaat gtaaccgata taccaaaacg tagcccactt aacatcgatt gtcaagcctt
     1501 cggtgaacag gagctgttgt tagattggcg ccactccggg ataatgttcc atgctctggg
     1561 tcggaacctg aaactgctat caactattga ggtgaatgac agtgaagatc cttgacctga
     1621 agataacacc gggtgtttct gtgagcctta tcgacctccc cctggatttg gatggctacc
     1681 acgacttctt cgggatctgg cttgtccggg acgaaggaag agaacggaat acccttgttg
     1741 atgtgggccc cacatcaacg gcttcgatcc ttatcgaaga gctccacaca cttgatgtta
     1801 aaaaactgga ctatatcctc cttacccata tccacctgga ccactccgga gggcttgccg
     1861 aactgctccg ggccttccct tcggcgaggg tggccgtcca cccgaaggga aagcgtcacc
     1921 tggtaaaccc cgaaaggctc tgggagagca gcctgcaggt cataccggag atggccctgg
     1981 cctacggtga accctctccc gtagacgaga gctgctttat ccccgaagag gtccctgtcc
     2041 ctggcataac cgccgtggac actcctggtc acgctcctca ccaccgctcc tttttctact
     2101 ggaccccgga aggtcaggtt ctctttgccg gtgaagcggc gagcaccttt acacgccttg
     2161 accacctggt cccaggagcg ggcgaaagaa ggtacatgct caggccggca tccccgcccc
     2221 gtttttacat cgaaactgcg ctgcgatcga tcgaggaact gaaaggcatt gatgcgtcct
     2281 tgctatgcta cgctcacttc ggttgcacgg agcaggtcag ggaaatgctg gaccatgcgg
     2341 gagagcagat ccgtctatgg agaaagcttt tcagtgagtt cctcaacatg aagggatata
     2401 aatttgagaa ccaggtggaa atgggggaac tgctcggctt cgttgtagaa agggatccat
     2461 ggcttgccga cttttcgctt cttccggcgg atgtaaggtc tcgcgagatg cggttcatgc
     2521 tttcaagcgc cgccggtttt ctgggggccg tcctggaaga gagaaaagta tagaaaggat
     2581 caccagtatt tctgcccttc gtggacgagc caccccggcc tgtgctttcc cgaggggtct
     2641 cgatgggtcc agtggataac ccctccttcg ggattccact cgtattcgcc actgaactct
     2701 atccggtcac cggtcctgag gccgtcgatc cggggagcca ggtctatgtt gtgggctatc
     2761 agcagggtct gcccggtcgg gagttccacc acgaagcgct ggtgcctgct ccccctgtcg
     2821 tcgtcaggaa gcagcctcgt aacgatccct ccagcctctg cctggactcc acccttcctt
     2881 tcccgataaa ggctctccaa tagctctgca cctgcagaga cctgttccgg aaccctgatc
     2941 gctgatggtc ccaaaaatcc tctttcggca aagacaagga ccagcgccgc cagaacggca
     3001 agaataacca gagatctcct tctgatggat ctcactcccc ttaacacagg tcttttttat
     3061 gaagaaattc ttcggttgta ttcctgcttc tcacagtacc attttgaaag actttcccaa
     3121 ggtgccattg aactcgtaag gtttgtcgag tacacaggat aaaggcagca gcccgatctc
     3181 tcctctcctg tttgtagccc tccgaacaga gcgcctggat ccccggtact cctgtacact
     3241 ataactcttg gaaggtatca cgtgaaaggg gggcccccct ttgccggaag ccccccttca
     3301 ggaagatttt ggacctggta agaccaggga caggtcctac gggagttcta tgaacttttt
     3361 ctcggtgagg catgcggtca ttttctgggc gtactggatc ttttcggtgg ctgtcttgag
     3421 gagttcctct ccggagagga cctttctggc gatcccctgc tcgatggcct tcatgccgac
     3481 ggcggtcgcc acctcggggt agacccaggc ctgatccatg gtcgggataa ggtagtcttc
     3541 ggagaggcca ttcttctccg cacactttga aagggtcttc gctgcggcta tgcacatctc
     3601 gtccgtaatg gtcgaggcct gcacgtcgag ggtgccccgg aagatccccg ggaagccgag
     3661 ggaattgttt atctggttcg ggaagtcaga ccttcccgtg gcaactatcc tcgctcccgc
     3721 ttctttcgca tcccagggcc atatctccgg gatggggttg gcgcaggcaa agagcatggc
     3781 atcctttgcc atggtcgcga cccactcctt cttgaccacg tcgggacccg gtttggtgta
     3841 gcagtagacc acatcagcat ccctgaagag gacctccggg ccgccttcga taccctcggg
     3901 gttggtggtc tgggcaaagt gccacttcac gtcgtagggg acgtccttca gctcggtccg
     3961 gcccttgtgg agcgtcccct tgctgtcggc catgataacg ttctccggcc tgaccccggc
     4021 gcttatgagg atcctggccg tggctatgct ggcagcaccg gccccgaaga aacgtacctt
     4081 cgcttcgtct atcttcttcc ccgtgatctt gagtccgctg tagagtcctg ccaggttaac
     4141 tgccgcagtt ccctgctggt cgtcgtgcca cacggggatc ttgcagtcct tcctcagttc
     4201 gtcaagtacg tggaagcact taggctgtgc gatgtcctcg aggttgatcc cgccgaaaga
     4261 tggcgcgatg agcctcaccg tctcgatgag cttgtcaggg tccttgagac ggtgaggctc
     4321 atcgcgccat ctttcggcgg g
//
LOCUS       DAOPKQ010000111         4320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_22275_length_4320_cov_4.086518, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000111 DAOPKQ010000000
VERSION     DAOPKQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22275_length_4320_cov_4.086518"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK146_04810"
     CDS             complement(<1..284)
                     /locus_tag="PK146_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163268.1"
                     /note="catalyzes the formation of acetyl-CoA from pyruvate
                     and coenzyme A; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HPE91595.1"
                     /translation="MAVKLKDLITKERAMRQGHRMCPGCGAPTVVQQALLAVEQPVVV
                     TAATGCLEVSTTIYPYNAWGVPFLHSAFENAGASVSGIEAAYVALKKKGK"
     gene            complement(298..1464)
                     /gene="porA"
                     /locus_tag="PK146_04815"
     CDS             complement(298..1464)
                     /gene="porA"
                     /locus_tag="PK146_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HPE91596.1"
                     /translation="MSRKDITSGNWAVAEAMRQINPDVVAAYPITPSTDIPMKFSEFV
                     ADGLVDTNLVTVESEHSAISACLAAAVSGARVMTASSANGVALMHEIFPIAASYRAPI
                     VFGLVNRSLGAPINIHCDHGDSMAERDSGWIHIYCEDGQEVYDTMVMAVRIAEHKEVL
                     LPVFVCQDGFITSHCFEPVEFMDDEEVRSFIGERDPLFPLLDIDNPVAYGSLALTDYY
                     FEIKRQQVEAMKNVEKVYREVAGEFAKKTGRDYPVVDSYKTDDADHIMVIMSSAAGAA
                     KDTVDRLREKGHKVGCMRIRLFRPFPAKAVVGALKGAKSIAVLDRAASLGAPAPLFTE
                     IKGALYDAGIHIPANTYVYGLGGRDFFPHNAEEAFMDLINNNLNPEERYLGLRE"
     gene            complement(1493..1789)
                     /locus_tag="PK146_04820"
     CDS             complement(1493..1789)
                     /locus_tag="PK146_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE91597.1"
                     /translation="MSSLKGWKDLPLATTVPASTSEKVQTGQWRSIRPVWNEEECIHC
                     LQCWIQCPDKSIKATPEGKVTGIDYFFCKGCGTCAAVCPKKAIEMRPESEFIEQ"
     gene            complement(1786..2361)
                     /locus_tag="PK146_04825"
     CDS             complement(1786..2361)
                     /locus_tag="PK146_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE91598.1"
                     /translation="MSKVIEVRWHGRGGQGAKTGSAILAEVLFEAGKHTQSFPEYGAE
                     RQGAPMKAFNRVSDRPIRDRSGVLNPDIVVLIDPTLIEAVRPTEGGKETTVYLINAPE
                     SPAIVKKKLGLKDSIKLMTDDATRITLEEFGQNRPNTPMLGALAHFFKDVPVDAFIEH
                     FTHKMGAKLPEKVLKANQNAIRRGYEEVQQG"
     gene            2668..3603
                     /gene="trmB"
                     /locus_tag="PK146_04830"
     CDS             2668..3603
                     /gene="trmB"
                     /locus_tag="PK146_04830"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPE91599.1"
                     /translation="MIPLGEILARRDPASMIQKICSQKAPFNAVEIGFGNGEFLQHAA
                     LERPDGFFFGIEVSINCVMKALKRTRRSSLSNVRLLLGDARFLLNECFPEDWLDAVYM
                     NFPCPWPKSRHAKRRVTSRGFGDALAGVLKIGGAFELVTDDERYAEDAAITIPSHPAI
                     EMEAFEADPLRKVRTKYERKWLEAGRRIYLLRFRKERSFRRKTLGGGVEELHKALERP
                     CPELRELSALTGREGGQREARWVFRDCYINPAGVVLLETLTSDEGFEQSFFFRIVPRG
                     LGCMVKVDPSTRPFTTPSVSGAFQEVVSFLGGGEP"
     gene            3600..4037
                     /gene="tsaA"
                     /locus_tag="PK146_04835"
     CDS             3600..4037
                     /gene="tsaA"
                     /locus_tag="PK146_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPE91600.1"
                     /translation="MNGYALHPVGFVRSRINRPVPPDDFRGIRSTIEILPEFEEALEG
                     LEGEDHILVLFRFHLSKGFSLKVHPRGDLNRPLRGVFSTCSPDRPNHIGTSLVRLSKV
                     EGATLTVEDLDAVDGTPVIDIKPFKGIDRRSKEQENSQGERDS"
     gene            4034..>4320
                     /locus_tag="PK146_04840"
     CDS             4034..>4320
                     /locus_tag="PK146_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPE91601.1"
                     /translation="MKQARPTSGLVLQALFNILGPGDGRSFLDLFSGTGRVGAEASRR
                     GFCPVVLVDSSRKGLPGTEGPGGLKGTEFLVLDVRRALLILEKRGRHFDVI"
BASE COUNT          908 a         1175 c         1248 g          989 t
ORIGIN      
        1 atcttgccct tcttcttcag ggcaacatag gccgcctcga tcccggagac gctggcgccg
       61 gcgttctcga aggcggagtg aaggaagggt accccccagg cgttgtaggg gtatatggtg
      121 gtggagacct ccaggcaacc cgttgcagcg gtgacaacaa cgggctgttc aacagccagg
      181 agcgcctgct ggaccaccgt aggcgcgcca caccccgggc acatcctgtg tccctggcgc
      241 atggctcttt cctttgtgat caggtctttc agtttgacag ccatcctggt gcacccccta
      301 ttccctaagg ccgaggtatc tttcctcggg gttcaaattg ttgttgatga ggtccatgaa
      361 ggcctcctcg gcgttgtggg ggaagaaatc ccttccgccc agtccataaa cgtatgtatt
      421 ggcagggatg tggataccgg catcataaag ggctcccttt atctccgtga agagcggtgc
      481 cggggccccc aggcttgccg ccctgtcgag aaccgcgatg gacttggcac ccttcagggc
      541 acccacgacc gccttcgccg ggaagggacg gaagagcctt atcctcatgc accccacctt
      601 gtgccccttc tccctcagac ggtccactgt atccttggcg gcgccggccg cagatgacat
      661 tatcaccatg atgtgatcgg catcgtcagt cttgtaggaa tccacgacag gatagtcgcg
      721 acctgtcttc ttggcgaact ctccggccac ctcccggtag accttttcca cgttcttcat
      781 tgcttcgacc tgttgccgct tgatctcgaa gtagtagtca gtcagggcaa gagatccgta
      841 ggcaacgggg ttatcgatat cgaggagagg gaagagagga tccctttcac cgatgaacga
      901 tctgacctcc tcgtcgtcca tgaactccac gggctcgaag cagtggctcg tgatgaatcc
      961 gtcctggcag acgaagaccg gaaggagaac ttccttgtgt tctgcgatcc tgacggccat
     1021 gaccatggtg tcataaactt cctggccgtc ctcgcaatag atgtggatcc acccggaatc
     1081 gcgctcggcc atgctgtctc catggtcgca gtggatgttg atcggcgctc ccagggacct
     1141 gtttaccagg ccgaagacta tgggtgccct gtaggaggct gcaataggga agatctcgtg
     1201 catcagtgcc acgccgttgg cgctcgacgc cgtcattacc cgtgctcctg atactgccgc
     1261 ggcaaggcag gcactgatcg cagagtgctc actctcgacg gtgacaaggt tcgtgtcaac
     1321 caggccgtca gcgacaaatt ccgagaactt cattggaatg tccgtagaag gggtgatggg
     1381 gtaggcggcg accacatcag ggttgatctg tctcatcgcc tctgccactg cccagttccc
     1441 ggaagtgata tcctttctcg acatcctgtt cacatccttt cgatcggcag ggctactgct
     1501 caatgaattc tgattcaggc cgcatctcta tggccttctt ggggcataca gcggcgcagg
     1561 tcccgcaccc cttgcagaag aagtagtcga tacctgttac cttcccttcc ggagtagcct
     1621 tgatggattt gtcggggcac tggatccagc actggaggca gtgaatgcac tcctcctcgt
     1681 tccagacggg gcgtatgctc cgccactgtc cggtctggac cttctcggag gtcgaggccg
     1741 gcaccgtagt tgcgagcggc agatctttcc agccctttaa cgaactcatc cctgctgcac
     1801 ctcctcgtat ccgcgacgga tcgcgttctg gttagccttg aggaccttct cggggagttt
     1861 tgctcccatt ttgtgggtaa agtgttctat gaacgcatca acgggaacat ctttgaagaa
     1921 gtgtgccaat gcacccagca tgggtgtatt gggtctgttc tgaccgaact cttcaagggt
     1981 gatcctggtc gcatcgtcgg tcatgagctt tatcgaatcc ttcagcccca gcttcttctt
     2041 gacgatcgcc ggtgactcgg gcgcgttgat gaggtagacg gtggtttcct tgcctccctc
     2101 ggtgggcctg acggcttcga tcagcgtggg gtcgatcagg accactatat ccgggttgag
     2161 gactccggaa cggtccctga tcggtcggtc cgaaaccctg ttgaaggcct tcattggtgc
     2221 tccctgacgc tcggctccgt actcggggaa agactgggtg tgcttgccgg cctcgaaaag
     2281 cacctccgcc aggatggcgg aacccgtctt ggctccctgt cctccccttc catgccatcg
     2341 aacttcgatg actttcgaca taaatacccc tccttcatgg aagatgcgtt ggaacggtta
     2401 attgcggtta atccaaaaag ccaactaaaa tgataacaca caataaaaca gatgctcccc
     2461 ggcaaacctt gcccctgggg ttgccgcagg cgggggagca ggaagaactg gatcctgaag
     2521 gttataatta ttaaccaccg ggaaggtccg gctcttcagg atatttcgta cagcgcatgt
     2581 atattgtatg taggcaaaac cttgtgtcaa gcctgaatag ttttacactt cttctcagct
     2641 gctcttttcg gaaggggagt gcctgtattg atacctctgg gtgagatcct ggcaagaagg
     2701 gacccggcaa gcatgatcca gaagatctgc agccagaaag cgccctttaa tgccgtcgag
     2761 atcggcttcg ggaacggcga gttccttcag catgcagcat tggagagacc cgatgggttt
     2821 tttttcggca tcgaggtctc catcaactgt gtaatgaagg ctctaaaacg gacacggaga
     2881 tcgtcgctga gcaatgtccg tttactactg ggcgatgccc gtttcctgct gaacgaatgt
     2941 ttccccgagg attggctgga tgctgtttac atgaacttcc cctgtccatg gccaaaaagc
     3001 aggcatgcaa agagaagagt gacatcccgg ggattcggcg acgctttggc gggcgttctc
     3061 aagattggag gggccttcga actggtgacg gatgatgaaa gatacgctga ggatgcagcc
     3121 attacgatac catcccaccc cgcaatagaa atggaagcct tcgaagcgga ccccttaaga
     3181 aaagtgcgca ccaaatatga aaggaagtgg ctggaagccg gcagaaggat ctacctcctt
     3241 cgtttcagga aggagagatc tttcagaaga aagactttgg gagggggagt ggaggaattg
     3301 cacaaggctt tggaaagacc ctgtcctgaa ctgagggaac tctcggcgct cacgggaagg
     3361 gagggaggtc agagggaggc caggtgggta ttcagggact gttatatcaa ccctgcaggt
     3421 gtggtactgt tggagactct gacctctgat gagggtttcg aacagtcctt cttcttcagg
     3481 atagttccga gaggcctggg gtgcatggtc aaggttgatc cttccactcg gccattcacc
     3541 actccatcgg tgtcaggagc ctttcaggag gtagtctcct tcctcggggg aggagagcca
     3601 tgaacgggta tgcccttcac cccgtcggtt ttgtccgctc tcgcataaac agaccggttc
     3661 cgcccgatga tttccgtggc ataagatcga cgatcgagat cctgcccgaa ttcgaggaag
     3721 ctttggaggg actggaaggg gaggatcaca ttctggttct tttcaggttc catctctcca
     3781 aggggttttc cctgaaggtc catccgagag gcgacctaaa caggccacta aggggtgttt
     3841 tctcgacctg cagccccgac aggcccaacc acatcggcac aagccttgtc aggctgagca
     3901 aggtggaggg tgccaccctg acggtagagg acctggacgc agtggacggt acccctgtaa
     3961 tagacatcaa acccttcaaa gggatagacc gcagatcaaa ggagcaggag aattcacagg
     4021 gggaacggga cagttgaagc aggcccgacc cacctccggc cttgtgctgc aggctttgtt
     4081 caatatcctt gggcctggtg acggcagatc cttcctggat ctcttttcgg ggacggggag
     4141 agtaggggcc gaggccagca gaagaggttt ttgcccggta gtcctggtcg attcctccag
     4201 aaagggtctt ccgggaacag agggacccgg aggtctaaag ggaacggagt tcctggtcct
     4261 ggatgtccga cgggccctgc tgatcctgga aaaaaggggc cgtcattttg acgtgatctt
//
LOCUS       DAOPKQ010000112         4316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_22310_length_4316_cov_4.249941, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000112 DAOPKQ010000000
VERSION     DAOPKQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22310_length_4316_cov_4.249941"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PK146_04845"
     CDS             complement(<1..509)
                     /locus_tag="PK146_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPE91602.1"
                     /translation="MGKVIGIDLGTTNSCVAVKEGDHITVITNAEGARTTPSVVAFTK
                     DKDRLVGQLAKRQAIVNPERTISSIKRKMGSDYRIDIDGVSQTPQQISAMILQKLRRD
                     AEEYLGEKIGQAVITVPAYFTDAQRQATKDAGKIAGLEVLRIINEPTAAALAYGMDKG
                     GENKILVFDL"
     gene            complement(568..1203)
                     /locus_tag="PK146_04850"
     CDS             complement(568..1203)
                     /locus_tag="PK146_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPE91603.1"
                     /translation="MSRPREKRDPGAPFEKKYGEAGVSAKDHCRDNDQNEPFEEGAKE
                     SSRAENEELLSELEAAKALAERLGEENRELGDAVARARADFFNFRQRIEREQEKLRAM
                     AGESAAAALIPVLDNLDRALSANRSDPDSFFRGVEMVRDQFFSVLQDLGVSAIETVGR
                     PFDPTVHEAVSVEETAEGVDDGTVTGEIQSGYTIAGKVLRAARVRVARRTA"
     gene            complement(1204..2244)
                     /gene="hrcA"
                     /locus_tag="PK146_04855"
     CDS             complement(1204..2244)
                     /gene="hrcA"
                     /locus_tag="PK146_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPE91604.1"
                     /translation="MLTERHLEVVLAVVYEYIKTGEPIGSRTIARKFLRDRSAATIRN
                     EMADLEEMEYLYQPHTSAGRIPTPKAYRVYVDAILRRQRPFPSVSEGWIRELRGRRKD
                     VEEALVYATRLLGRITSYIGVAALGPAQETVLQRVEFLRMGHSTVLVLLVLRGGLIHH
                     KTVTLPCDMTQEALDELSRRVSTIAIGRSWAEVRGTLQSYVEDALERYADSCRSALKE
                     MDGLLTEQPFQFFTGGTHNVLGLPDFSDLGRLQTIMALLEEDESIGKLVRNCSTERGL
                     CVTIGDENPELKMPDCSIVMASTVTGGRKAVVGLIGPVRMDYERSISVLERVLETLAG
                     KNENVKSDEEVH"
     gene            2441..3088
                     /locus_tag="PK146_04860"
     CDS             2441..3088
                     /locus_tag="PK146_04860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPE91605.1"
                     /translation="MTKNNRTTTVILIRHGECEGNREGLFRGRTNFPLNGTGREQARS
                     LAEAISYLSPSMVFSSPLTRATETATIISERCGAEMSIRQGFNNMALGKWENRKKTDI
                     EQEYPEEWRLWLSHPERLQIAGGESLLDVRRRAFANLDTLVKHNLGGTFAVVTHRAVL
                     KPLLAEVLDLGDPYFWKIHLDTASFSVLKHEEDRGYCLVSLNQTYHLERFISEWV"
     gene            3132..>4316
                     /gene="folP"
                     /locus_tag="PK146_04865"
     CDS             3132..>4316
                     /gene="folP"
                     /locus_tag="PK146_04865"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPE91606.1"
                     /translation="MGIYRIEIDNDSDLVHLIHTTGADERSFCFFQRKSRMLSFFISN
                     VDFRAANALKQELLSRGGDAIVHRCAIEGSIPTSNVVILGTLKTLEDLSDKLRSMPYW
                     GLDGIRSGLVQAISSIGIRRWRLNIPDREDLELGGRTKVMGIINVTDDSFYSGSRVDG
                     PERCVEIALRMHEEGADILDIGAESTRPGSSPPSQEEEACRLIPAIAAVRREIPRAII
                     SADTFRTGTAEAALDSGADIINDISGGAFDKMMFPLLAKRGNPFIIMHARDVPGGMHH
                     PVSYKDVIGDILAFFSGQILKATKAGVNLEQIILDPGIGFSKNAEQSLEVLKGVASFS
                     TLARPLLIGHSRKSTIGKVLGLENPEDRLEGTLALSAFCAMKDVALIRVHDVAENIRA
                     VRM"
BASE COUNT          919 a         1233 c         1187 g          977 t
ORIGIN      
        1 agatcgaaga cgagtatctt gttctcgcca cccttgtcca taccgtaggc cagggccgcc
       61 gcggtgggct cgttgattat cctgaggacc tcaaggcctg ctatctttcc ggcatctttc
      121 gtagcctgcc gctgggcgtc ggtgaaatag gcaggtacgg ttattaccgc ctggccgatc
      181 ttctctccca gatattcctc ggcatctcgt ctgagcttct gcaggatcat ggccgatatc
      241 tgctgcgggg tctgggagac gccgtcgata tcgatcctgt agtccgagcc catcttcctt
      301 ttgatagagg agatggtcct ctcggggttg acgatagcct gcctctttgc aagctgaccg
      361 accagcctgt ccttgtcctt tgtgaatgcc actaccgatg gggtcgtcct cgcgccctcg
      421 gcgttggtaa tgaccgtaat atggtcgcct tccttgacgg ccacacagct gttagttgtt
      481 ccaagatcga tgcctattac ctttcccatt cagtattcac cccgcatgga tttaaaaaat
      541 gaagttccct tgatgacctt tgacccctca ggcggttctc ctggcaaccc ttacccgggc
      601 ggccctgagg acctttccgg cgatggtgta ccccgactgg atctccccgg tcacggtccc
      661 gtcatctacc ccctccgcgg tctcttccac tgacacagct tcatgcacgg tgggatcgaa
      721 gggtctgccg acggtctcga tagctgagac ccccagatcc tggaggaccg aaaagaactg
      781 gtccctgacc atttcgacac ccctgaagaa agaatccggg tcggacctgt tggccgaaag
      841 ggccctgtcc agattgtcaa ggacagggat aagagcagca gcagcgctct ctccagccat
      901 ggccctgagt ttttcctgct cgcgttcgat ccgctggcgg aagttgaaga agtcagccct
      961 ggctctggct accgcgtcgc ccagctccct gttctcttca cccagacgtt ccgccagggc
     1021 ctttgccgcc tccagttccg aaaggagctc ttcattctcc gctcgagagc tctcctttgc
     1081 cccttcctca aaaggttcat tctgatcatt atccctgcag tgatcctttg cggacactcc
     1141 ggcctcccca tatttctttt caaagggagc cccggggtcc cttttctctc tgggtcttga
     1201 cattcaatgt acctcctcat cgcttttcac gttctcgttc ttcccggcca gagtctccag
     1261 aacacgctca aggaccgaga tggacctctc gtaatccatc cttaccggcc ctatgagacc
     1321 gacgacggcc ttccgaccgc cggtgacggt ggaagccatc actattgagc agtctggcat
     1381 cttcagctcc gggttctcgt cccctatggt cacgcagaga cctctttctg tagagcagtt
     1441 acggaccagc tttccgatcg actcatcctc ctcgaggagg gccatgatgg tctggagacg
     1501 gcccaggtcg ctgaagtcgg ggagacccag tacattgtgg gtaccgcctg tgaagaactg
     1561 gaaaggctgc tccgtaagca ggccatccat ctccttcaga gccgaccggc aactatctgc
     1621 atatcgttca agggcatcct ccacatagga ctggagagtt cctcttacct ccgcccagga
     1681 cctgccaatg gctattgtgg acacccgtcg ggaaagttcg tcaagggctt cctgggtcat
     1741 gtcgcagggg agggtcacgg tcttgtggtg gataagccca cccctgagca ccagcagaac
     1801 aagtaccgtg ctgtgtccca ttcgcaggaa ctccaccctt tgaagaaccg tctcctgggc
     1861 tggccccagg gcagcgacgc caatgtaact ggtgatcctc cccaggagcc ttgtggcgta
     1921 gaccagggct tcctccacgt ctttcctccg tccacgtaat tccctgatcc acccttcgct
     1981 gacggaggga aagggcctct gtcttctaag gatcgcatcc acgtataccc ggtatgcctt
     2041 tggagtgggg atccttccgg cggaggtatg gggctggtag agatactcca tctcctccag
     2101 gtcagccatc tcgttcctga tggtggctgc gctacggtct ctcaggaact ttcttgcgat
     2161 ggtcctggaa cctatgggtt caccggtctt gatgtactcg tagaccacag ccaggaccac
     2221 ttcaagatgt ctttcggtca acacttggaa gcaaccccct gttttttgat ttattagcac
     2281 tcccattcac agagtgctaa agggatctcg cccgaatggt agattagcaa tggcatcgtc
     2341 tattgtcaac actggtcaga aacttgatgc ctccataatg tgctctcatt gacctagaat
     2401 agggctggga aaaacatccc tctcttcagg aggttcccct ttgacgaaga ataacaggac
     2461 aacgacagtg atcctgatca ggcacggaga gtgcgaaggg aacagggaag gtctcttcag
     2521 gggaaggacg aacttcccac tcaacgggac cgggagggaa caggcacggt ctctggccga
     2581 agcaataagt tacctgtccc cttcaatggt attctcgagt ccgctgaccc gtgcgacaga
     2641 aacggccact atcatctcag aaagatgcgg ggccgagatg tccataaggc aggggttcaa
     2701 caacatggcc ctgggcaagt gggagaacag gaaaaagacc gatatcgagc aggagtaccc
     2761 cgaggagtgg agactatggc tctctcaccc cgaaaggctt cagatcgccg ggggagagtc
     2821 ccttcttgac gtcaggagga gggccttcgc gaacctggat accctggtca aacacaattt
     2881 ggggggcact ttcgcggtcg tgacccacag ggcagtgctc aagccgcttc tggcagaggt
     2941 gctggacctg ggtgatccct acttctggaa gatccacctg gacactgctt ccttttccgt
     3001 cctgaaacat gaggaggaca ggggatactg ccttgtctcg ctcaaccaga cataccatct
     3061 ggaacgattc atcagcgaat gggtttgaga tggataaaga ttttcactcc gggaaaggga
     3121 gatcttccag tatgggcatc tacaggatag agatcgataa cgacagtgat ctggtccacc
     3181 tgatacacac taccggagct gacgagagaa gcttctgctt cttccagcgc aagagccgga
     3241 tgctgtcttt ctttatttcc aacgtagact ttcgggctgc caacgccctc aagcaggaac
     3301 tgctctcacg gggcggggac gcgatcgtcc acagatgtgc gatcgaaggt tcgataccga
     3361 cgtcgaacgt ggtgatcctg ggcaccctga aaaccctcga ggacctttct gacaagctca
     3421 ggtccatgcc ctactggggc ctggacggga taaggtccgg cctggtccag gcgatctcct
     3481 cgatcgggat caggagatgg agactgaata tcccggaccg ggaggatctg gagctgggcg
     3541 gaaggaccaa ggtaatgggt attatcaacg tcactgacga ttcgttctac tccggaagca
     3601 gggtcgatgg accggaaagg tgtgtcgaga tcgccctgag gatgcacgag gagggcgctg
     3661 atatacttga tatcggtgcc gaatcgacgc ggcctggttc ttccccccct agccaggagg
     3721 aagaagcatg tcgcctgatt ccggcaatag ccgccgtaag aagggagatc cctcgagcga
     3781 taatctctgc tgacactttc aggaccggga ccgctgaggc tgcccttgat tcaggagccg
     3841 atattatcaa cgatatatcc gggggggcct tcgataaaat gatgttcccc cttctggcaa
     3901 agagaggaaa cccgttcata ataatgcacg ccagggatgt tccagggggg atgcaccatc
     3961 ctgtatccta caaggatgtg ataggcgata tccttgcttt cttctccggg cagatactga
     4021 aggccaccaa ggcaggggtg aacctcgaac agataatact ggatccgggg atagggttct
     4081 ccaagaacgc cgagcagagc ctggaggtgc tcaagggagt ggcctccttc tccacactgg
     4141 caaggcccct gctcataggt cactcgcgga agagcactat cgggaaggtt ctgggtctgg
     4201 aaaaccccga ggacaggcta gaggggaccc ttgccctgtc ggccttctgt gccatgaagg
     4261 atgtggccct gataagggtg cacgatgtgg cagagaatat aagggccgta aggatg
//
LOCUS       DAOPKQ010000113         4316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_22311_length_4316_cov_4.075100, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000113 DAOPKQ010000000
VERSION     DAOPKQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22311_length_4316_cov_4.075100"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PK146_04870"
     CDS             <1..256
                     /locus_tag="PK146_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPE91607.1"
                     /translation="VKEGIEEVVLRGTGRRAGQYGVSVAGKTGTAENPHGDDHAWFVG
                     YAPVENPRYVAVALVEAGLHGSSAAGPVVGELLGYLCRFD"
     gene            281..961
                     /gene="minC"
                     /locus_tag="PK146_04875"
     CDS             281..961
                     /gene="minC"
                     /locus_tag="PK146_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HPE91608.1"
                     /translation="MAIKGGKEDLELRGEGGHVRLIIPQRLRGKDLRLESRGFLEKAS
                     GILKDASVVVDLKGSGWDRGDILSIVESILLPVGASVLSWSPEEEDVRSWMKREGFAL
                     YLSGTEEKARMQPRPLVESLVIDESLRSGRKIDHDGDIMILGNVNEGAEVFAGRSVVV
                     VGRLRGLAHAGLTGPEEAFIVSGQFEASQVRIGDRISYMDEHCAWWGKTVSMKVVGGS
                     IVVREIVP"
     gene            1009..1824
                     /gene="minD"
                     /locus_tag="PK146_04880"
     CDS             1009..1824
                     /gene="minD"
                     /locus_tag="PK146_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HPE91609.1"
                     /translation="METRIIVVTSGKGGVGKTTVTANLSARLAMEGFKVVAIDADIGL
                     RNLDVVMGLENRIVFNMIDVIEGACKLPQALVRDKRVENLYLLPAAQTRTKDAVTSDQ
                     MKELCDSLRGEFDFVLVDSPAGIESGFRNAAAGADEALVVATPEVSSVRDADRIIGLL
                     ESFGKTSINLIVNRVRPEMVRSGKMLGVSDVMEILAIDLIGIVPEDDSVVVSTNKGEP
                     LAMTNVSPASRAFEKIAGRIMGKDIPLQDIDDLEEKGFLVNFRKLFGRRGGRS"
     gene            1828..2103
                     /gene="minE"
                     /locus_tag="PK146_04885"
     CDS             1828..2103
                     /gene="minE"
                     /locus_tag="PK146_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HPE91610.1"
                     /translation="MGFLDRFFRRETPRKSAKERLQLVLIHDRADISPGTMELIRKDL
                     IKVISNYMVISEEGIEMDLERDGRSVALVASIPVLEVKRKRSREGDK"
     gene            2100..3215
                     /gene="rodA"
                     /locus_tag="PK146_04890"
     CDS             2100..3215
                     /gene="rodA"
                     /locus_tag="PK146_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPE91611.1"
                     /translation="MTGLGALREDLSRLKDIDRVLVAAVIPLLAIGVVSIYSALAGRG
                     EVAVSMALRQVVWSVMGVVAFMITFAAGYERMFRYGYWLYAGALLTLTALLIAGYTAK
                     GATSWFDFGAFRFQPSEPTKLALSIILAVFLCRYPPKTLVNILGVLLLSSFATVLVLL
                     QPDLGGVIVYAFMIFVALAVAGTPRKYLLLLAGIALLSLPVGWSMLKEYQKLRMLVFL
                     NPSIDPLGAGYNVIQSRIAVGAGGLFGKGFLQGSQSKLHFLPEAHTDFVFSVFSEEFG
                     FVGASIVLFLFAVLLWRMIRTAARSGDQRGKILVAAISAWIWFQIFENIAMTVGIAPV
                     TGLALPFMSYGGSSLVSLGAALGLVQSVHVSARPQYE"
     gene            3237..>4316
                     /locus_tag="PK146_04895"
     CDS             3237..>4316
                     /locus_tag="PK146_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPE91612.1"
                     /translation="MPFIFDELEDPIWQMLSSVKRPSRYAGGEWGADGGLVEGEERSS
                     ICLAFPDVYEVGMSYLGYQILYNMASGIPGVRVERVYCPWPDAEAYMRENRMALCSLE
                     SGRPISSFDVVGFTLQYELTSTNILTMLDLGGVPLKASERGEKDPLVVAGGPGAFAPE
                     PLVPFFDVFCVGDGELLLPDLLSLFPTRSREDVLRGLAKTEGFYVPAFHEGRSVRRRV
                     LMDLDGGFAPSGMIVPSSEIIHDRVGVEVFRGCTRGCRFCQAGVVSRPVRERSPERVA
                     GIARDLLAMSGYDEVGLVSLASCDYSGIERVVDLLGPDLSARNFRLSLPSLRMDAFSV
                     ELADHLEELGRGGLTFAPEAGTQRLR"
BASE COUNT          840 a         1178 c         1397 g          901 t
ORIGIN      
        1 cgtgaaagag ggcattgagg aggtcgttct ccgtggtaca gggcgcaggg ccggacagta
       61 cggcgtctcc gttgcaggaa aaacaggcac cgccgagaac cctcacggtg acgaccatgc
      121 ctggttcgtg gggtacgccc cggtcgagaa ccccaggtac gtagcggttg cccttgtcga
      181 ggcggggctt cacggaagtt cggcggcggg acccgtggtg ggcgaactcc tcggctatct
      241 ctgcagattc gactgacccg aaagggggac ctgatagata atggccatca agggtgggaa
      301 ggaagatctt gagcttagag gggagggcgg gcacgtaaga ctgatcatcc cccagcgatt
      361 gagagggaag gatctccgtc tggagtccag aggttttctc gagaaggcct ccggcatcct
      421 caaggatgct tcagttgtag tggacctgaa ggggagtgga tgggacaggg gtgatatcct
      481 gtccatagta gagtctatcc tgttgcccgt cggtgcgtcg gtcctctcct ggtccccgga
      541 ggaggaagat gtcaggtcct ggatgaaaag agaaggtttt gccctttacc tttccggcac
      601 tgaggaaaag gccaggatgc aaccccgccc cctggtggag agcctcgtga tagacgaatc
      661 cctcaggtcc gggaggaaga tcgatcacga cggcgacata atgatcctcg ggaacgtcaa
      721 cgaaggagcc gaagtgttcg ccggcaggtc tgtggtcgtt gtcgggcgcc tccgcggact
      781 tgcccacgcg gggttgaccg gccccgagga ggcctttatc gttagtggac agttcgaggc
      841 cagccaggtg aggatcgggg acaggataag ctacatggat gagcattgcg cctggtgggg
      901 gaagaccgtc agtatgaagg ttgtcggcgg atctatagtt gtcagggaga tagtaccgtg
      961 agcggcaagg gaaaagtcgg cccatcggct gaaatggagg aatctagcat ggaaacccgg
     1021 atcatcgtag ttacttctgg taagggggga gttggcaaga ccactgtcac ggccaacctc
     1081 tccgcccgcc tcgccatgga gggcttcaag gtcgttgcca ttgatgccga tatcggtctg
     1141 cggaaccttg acgtcgttat gggtcttgag aacaggatcg tcttcaacat gatcgacgtc
     1201 atcgagggtg cgtgcaagct cccgcaggcc ctggtgaggg acaagcgcgt ggagaacctc
     1261 tatcttctcc cagctgccca gaccaggacc aaggatgccg ttacgtcgga ccagatgaag
     1321 gaactctgcg actccctgag gggcgagttc gatttcgtcc tcgtcgacag cccggccggg
     1381 atagagtctg gtttccggaa cgccgccgcg ggagccgacg aggccctggt ggtcgcgaca
     1441 ccagaggtgt cctctgtgcg ggatgccgac aggatcatcg ggctactgga gtctttcggc
     1501 aagacttcca tcaacctcat cgtgaaccgg gtaaggcccg agatggtcag gagcgggaag
     1561 atgctcgggg tctcggacgt gatggagatc ctcgccattg acctgatagg catagtacca
     1621 gaggatgaca gcgtcgtcgt ttcgaccaac aagggagagc ccctggccat gaccaacgtc
     1681 tctcccgctt cgagggcctt cgagaagatc gccggaagga tcatggggaa ggacatccca
     1741 ctccaggaca tcgacgacct ggaagagaag ggttttttgg tcaacttcag aaagctgttc
     1801 ggacgtcgcg gagggaggag ttagatcatg gggttcctgg accggttctt ccgcagggaa
     1861 acacccagga aatcagccaa ggagaggctc cagctggttc ttatccacga ccgggccgac
     1921 atttctcccg gcacgatgga gctgataaga aaggacctta tcaaggtcat aagcaactac
     1981 atggtcatat ccgaagaagg tatcgagatg gacctcgaga gggatgggcg atccgtcgcc
     2041 ctggtggcga gcatcccagt gctggaggtc aagaggaagc gatccaggga aggagataaa
     2101 tgaccggact tggggcgctt cgcgaagatc tttccaggct gaaggatatc gacagggtcc
     2161 ttgtcgctgc agtgatccca ctcctggcta tcggcgttgt ctccatctac agcgctcttg
     2221 ccgggagggg tgaagtggcg gtgagcatgg ccctgaggca ggtggtctgg agtgtcatgg
     2281 gggttgtggc cttcatgatc acctttgctg ccgggtacga gaggatgttc aggtacggct
     2341 actggctcta cgccggagcg cttctgacac tgacagctct gctgatcgcc ggatataccg
     2401 ccaagggggc cacaagctgg ttcgacttcg gtgctttccg ttttcagcca tcggaaccga
     2461 caaaactggc cctgtcgatc atacttgccg tcttcctctg ccgttacccg cccaagaccc
     2521 tggtcaacat cctgggggtc ctcctcctgt catcttttgc cactgtcctg gtcctcctgc
     2581 agcccgacct tgggggagtg atagtctacg ccttcatgat cttcgttgcc ctggcggtgg
     2641 ccgggactcc caggaaatat ttactgctcc tggcagggat agcgcttctc tcgctgccgg
     2701 tcggatggtc aatgctcaag gagtaccaga aactgaggat gctcgtcttc ctgaacccct
     2761 ccatagaccc cctcggggcc gggtacaacg tcatacagtc ccgcatagcc gtcggcgccg
     2821 gcggtctttt cgggaagggg ttcctccagg gctcccagag caagctccat tttttgcctg
     2881 aagcccatac tgactttgtc ttcagcgtct tctccgagga gttcggtttc gtcggtgcct
     2941 ccatcgtcct tttcctcttc gcggtcctgc tgtggaggat gataaggaca gcggcgagat
     3001 cgggcgacca gagggggaag atcctggtgg cggcgatatc ggcctggata tggttccaga
     3061 tcttcgagaa catcgccatg acagtcggga tcgccccggt gaccggtctt gcgcttccct
     3121 tcatgagtta cggagggagc tccctggtct ccctgggggc cgcactcggc cttgtccaga
     3181 gcgtccatgt ttcggcgagg ccccagtatg agtgaagaat tcatggaggc gttctgatgc
     3241 cctttatatt cgacgaactg gaagacccca tctggcagat gctctcatcg gtgaagagac
     3301 cctccaggta tgccgggggc gagtggggtg ccgacggagg ccttgtcgag ggggaggaga
     3361 ggtcatccat ctgtcttgcc tttcccgacg tctacgaggt cggcatgagc tacctgggct
     3421 accagatcct ctacaacatg gcatcgggaa tacccggcgt cagggtggag agggtctact
     3481 gcccatggcc ggatgcagag gcatacatgc gcgagaaccg catggccctt tgctccctcg
     3541 agtcgggaag gcccatctct tccttcgacg tggtaggttt cacccttcag tacgagctga
     3601 ccagcacgaa catcctcacc atgctcgatc tggggggggt ccccctgaaa gcctccgaga
     3661 ggggggagaa ggaccccctg gtagtggcag gcggccctgg tgccttcgcg ccggagccgc
     3721 tggtcccatt cttcgacgtc ttctgtgtcg gcgacgggga actcctgctc ccggaccttc
     3781 tctctctctt cccgaccagg tccagggaag atgtcctgag aggtctcgcg aagacggagg
     3841 gtttctacgt tcctgccttt catgaaggcc ggtctgtgag aaggagggtg ctgatggatc
     3901 tggacggggg tttcgccccg tcggggatga tagtcccctc cagcgagata attcatgacc
     3961 gggtcggagt agaggtcttc agggggtgca ccaggggttg ccgcttctgc caggcaggag
     4021 tggtctccag gcctgtacgg gagaggtcgc ccgagagggt ggccgggatc gccagagatc
     4081 ttctggcaat gtccggctac gacgaagtcg ggctggtatc gctcgcatca tgcgactaca
     4141 gcggcatcga gagggtggtt gacctcctgg ggcctgatct ttcggccaga aacttccggc
     4201 tatcccttcc cagcctcagg atggacgcct tctcggtcga actcgccgac cacctcgagg
     4261 aacttggcag gggtgggctt acctttgcgc cggaggccgg gacccagagg ttaagg
//
LOCUS       DAOPKQ010000114         4300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_22413_length_4300_cov_5.010836, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000114 DAOPKQ010000000
VERSION     DAOPKQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22413_length_4300_cov_5.010836"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="PK146_04900"
     CDS             complement(<1..1470)
                     /locus_tag="PK146_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE91613.1"
                     /translation="MLRTLLERSPEGLVFCDASSRICQVDDEFCRIFGYERMEILGKD
                     LDEVVSRDPLLIEEGRGLFSEFTSRGSVSVETERQRSDGSRFPVSITSFPVSANGSYC
                     GFFTIYRDITERRRYEREITSREERMKEILDSLPELVLRFTPDLVASYANRTYCEYHG
                     ITYKEAVGKSFENHILPDDIPAVFDKLMSLTKENPVITGVERNVMADGEIRWLEWTDR
                     GIFDQNGKLTEIQSMGRDITERQRTEEALAFERESLNALFENAMEGIVFCDHEGIVLK
                     ANPAFCDMFDYSREEVIGNLVDDLVAKGSEFMEEARDLSATTARGKSIAAEATRLKKD
                     GTPIQVSFLGVPVILSDGRIFGYGIYRDITAQKTAERALRESEAKYRDLFSTMPDGFY
                     ASTPEGYFIDANPAFIRMLGYDNLEELRSVYIPTDIYVHESERIQMHNDNYNEEFVDR
                     FETYRLKRKDGKIIWVEDFARYIKDGRGRVLFNQGICREI"
     gene            1733..3013
                     /gene="eno"
                     /locus_tag="PK146_04905"
     CDS             1733..3013
                     /gene="eno"
                     /locus_tag="PK146_04905"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPE91614.1"
                     /translation="MKKTITAVRAMEILDSRGNPTVRTWCRLANGVEVSASVPSGAST
                     GENEAVELRDGDRSRYGGKGVIQAVENVNGVIAPRLVGMDPTRQEDIDRLMIELDGTP
                     NKGKLGANAILGVSMAVARAASEALGLPLYEYIGGPEAARLPVPMMNILNGGKHADNS
                     VDLQEFMVMPIGAPTFAEALRYGTETFHALKKILSARGYATSVGDEGGFAPDLKSNDE
                     ACEVIVEAIEAAGYSPGRDVAIALDPAASSFFQNGSYVLSKSGQGSLTSRQMTALYRE
                     WVEKYHIVSIEDGLDENDWKGFREQTAELGGRVQIVGDDLFVTNSRFVERGIRERAAN
                     AVLIKLNQIGTVTETIETITMCRKAGWGYVISHRSGETEDTFIADLAVAMGGGQIKTG
                     SACRSERMAKYNRLLEIEAELDGKAVFEDPFKVR"
     gene            complement(3025..>4300)
                     /locus_tag="PK146_04910"
     CDS             complement(3025..>4300)
                     /locus_tag="PK146_04910"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HPE91615.1"
                     /translation="VFPVRLSREDVDSFYYGFSNEVVWPLFHDLQTRCRFEPEYWKSY
                     RRVNGLFARAVSRYTRAEDFVWVHDYQMIPVARMLGSSGVKRKCVFFLHIPFPPPDIF
                     LKLPWREELLKDFLEYDFVGFQTMRDRRNFTDCLRALIPGWKVSGRGPVATVSSGGRR
                     IRVAAMPISIDYRSFRDMAKSARISRILKDLKRQLPREKIAIGVDRLDYTKGIPQRIR
                     AFGEALRSFPELRERITFIQIVVPSREDVPAYHELREEIERLVSSVNGEFSTPGWVPV
                     HYMFKSIPREELVAYYRLADIALVTPLKDGMNLVAKEYCACQVDDDGVLVLGEFAGAA
                     AQMRRGAILVNSYDIQGMAKAINEAVNMPVEKRKGRMRRLRAGIRRQNVFWWVDNYLR
                     ASTGKALVDFPETDMAPVFHALIRRSPESQED"
BASE COUNT          890 a         1217 c         1261 g          932 t
ORIGIN      
        1 tatctccctg cagatcccct ggttgaaaag gaccctgccc ctgccgtcct ttatgtacct
       61 ggcgaagtcc tcgacccaga tgatcttccc gtcttttctc ttcagacggt atgtctcgaa
      121 acggtccacg aactcctcgt tgtagttgtc gttgtgcatc tgtatccttt cgctttcgtg
      181 gacgtagatg tcagtgggga tgtagacgga gcgcagctct tcgaggttgt cgtatccgag
      241 catccggata aaggcgggat tcgcgtcaat gaaatacccc tcaggggtgg atgcgtagaa
      301 gccgtcgggc atggtgctga agaggtcccg gtatttggcc tcgctctccc ggagggcacg
      361 ctcggccgtc ttctgggcag taatgtctcg gtatatgccg tatccgaaga tccgcccgtc
      421 cgagagaatg acgggaacac caaggaatga tacctgaata ggcgtaccgt ccttcttcag
      481 tctggtcgcc tcggcggcta tgctcttgcc ccttgcagtg gtagccgaca ggtccctggc
      541 ctcctccatg aactccgaac cctttgctac caggtcatcg accaggttgc ctatgacctc
      601 ttctctggaa tagtcgaaca tatcgcagaa tgcggggttg gctttgagga cgatcccttc
      661 atggtcgcag aagactatgc cctccatagc gttctcgaag agggcgttaa gcgactcgcg
      721 ttcgaatgcc agggcttcct ctgtcctctg ccgttcggtt atgtccctcc ccatggactg
      781 gatctccgtc agcttcccat tctggtcgaa tatcccccgg tcggtccact caagccaacg
      841 gatctcaccg tccgccatta cgttcctctc cacccctgtg atgacagggt tctccttggt
      901 gagggacatc agcttgtcaa agacggcagg tatgtcgtcg ggtagtatgt ggttctcgaa
      961 actctttcct actgcttctt tgtaagttat accgtggtat tcgcagtagg tcctgttggc
     1021 gtaggaggcg accaggtccg gcgtgaacct caggaccagt tccgggagtg agtccaggat
     1081 ctccttcatc ctttcctccc tggaggtgat ctctctctca tagcgccttc gctcggtgat
     1141 gtccctgtag atggtgaaga aaccgcaata gcttccgttg gccgagaccg ggaaggatgt
     1201 tatggacacg gggaaccggc tgccgtcact tctctgcctc tcggtctcga cggagaccga
     1261 acccctgctg gtgaactcac tgaagagacc ccttccctcc tctatgagca aggggtcccg
     1321 ggagaccact tcgtccaggt ccttgccgag gatctccatc ctttcgtagc cgaagatccg
     1381 gcagaattcg tcgtccacct ggcatatccg gcttgaggca tcacaaaaga caagcccttc
     1441 aggagatctc tccagaaggg ttctgaggat agcatcgttg aggctttccg ttaaagggac
     1501 agggctttgg tccggcagaa gcgctacctc caggcattat tcgtttctgg attataccac
     1561 ggctaaaagg ggaaggggct ggtcctgctg tgtatcctgc gaactatcct cgtgtccggg
     1621 acagatcata gagcgaaggg tctgctgaaa gaaacgttaa ttctgctata gttacaatgc
     1681 aggtgccgtt ttgcctggca gttctgaaat caaaggaaag ggagagaccc taatgaagaa
     1741 gaccatcacg gcagtaaggg caatggagat cctggattcc aggggcaatc cgactgtccg
     1801 tacctggtgc aggctcgcca acggtgtcga ggtttcggcc tcggtcccct cgggggcatc
     1861 gacgggagag aacgaggctg tagagcttcg tgacggcgac cggtccaggt acgggggtaa
     1921 gggtgtcatc caggccgttg agaacgtgaa cggtgtcatc gccccacgcc ttgtcgggat
     1981 ggatcctacc cgccaggagg atatagaccg gctcatgatc gaactggacg gaactccgaa
     2041 caaggggaag ctgggagcca acgcgatcct gggggtgtcc atggcagtcg cgagagccgc
     2101 ctcggaggcc ctgggtctgc cgctctacga gtacattggc ggtccagaag cggcaagact
     2161 gcctgtcccc atgatgaaca tccttaacgg gggcaagcac gccgacaaca gcgtggacct
     2221 ccaggagttc atggtaatgc ccatcggggc gccaaccttt gccgaagccc tgcgctacgg
     2281 gaccgagacc ttccacgcac tgaagaagat cctttctgcc agaggttatg ctaccagcgt
     2341 cggcgacgag ggggggttcg ccccggacct caagagcaac gacgaagcct gcgaggttat
     2401 tgtcgaggcc atcgaggcgg caggttattc cccgggcagg gacgttgcga tagcgctgga
     2461 ccctgcagcg agctccttct tccagaacgg gtcctacgtt ctttccaagt cgggacaggg
     2521 tagcctgacc agccgccaga tgacagctct ctaccgggaa tgggtggaaa agtaccacat
     2581 agtctcgata gaggacgggc ttgacgagaa cgactggaag ggtttccggg agcagacggc
     2641 agagcttgga gggagggttc agatagttgg ggatgacctc tttgtgacca actcccggtt
     2701 cgtggaaagg ggtatccgcg agagagcggc caacgcagtc ctcataaagt tgaaccagat
     2761 aggtacagtc acggagacga tcgagacgat cacaatgtgc agaaaggccg gttggggtta
     2821 tgtcatctcc cacaggagcg gagagacgga ggacactttc atcgccgatc tcgccgtggc
     2881 tatggggggc gggcagatca agaccggttc ggcctgccga agcgagcgca tggcaaagta
     2941 caacaggctt ctggagatag aggccgaact tgatgggaag gccgtcttcg aggatccctt
     3001 caaggtcaga taagggactg ccggtcagtc ttcctgtgac tccggtgagc gcctgatcag
     3061 ggcgtggaaa acgggggcca tgtcggtctc tggaaaatcc accagggctt tgcccgttga
     3121 agccctaagg tagttgtcca cccaccagaa gacgttctgc ctgcggatgc ccgccctcag
     3181 gcggcgcatc cgcccctttc tcttttcaac gggcatgttc acagcttcat tgatagcctt
     3241 tgccatcccc tgaatgtcgt aggagttcac caggatagcg cctcttcgca tctgagcggc
     3301 cgcccccgcg aactcgccaa gcaccagcac cccgtcgtca tcgacctggc aggcgcagta
     3361 ctccttggcc accaggttca tcccgtcctt gaggggtgtc acgagcgcta tgtctgcgag
     3421 cctgtagtag gctaccagct cttcgcgggg gatcgacttg aacatgtagt ggaccggaac
     3481 ccatccagga gtggagaact ctccgttaac ggagcttacc agcctctcga tctcctccct
     3541 cagttcatgg taggctggta catcctcgcg gctcgggacc acgatctgta tgaaggtgat
     3601 cctctcccgc aattccggga aggacctgag ggcctcgccg aaagccctga tcctctgggg
     3661 aattcccttg gtgtagtcca gccggtcaac ccctatggcg atcttctccc gtggaagctg
     3721 cctcttcagg tccttgagga tccttgagat cctggcagac ttggccatat ccctgaagga
     3781 tctgtagtcg atgctaatgg gcattgccgc caccctgatc cgccggccac cggaggagac
     3841 agtagcaaca ggaccacggc cggaaacctt ccagccgggg atgagggccc tgaggcagtc
     3901 ggtgaagttc ctcctgtccc tcatggtctg gaaccccacg aagtcgtact cgaggaagtc
     3961 cttcagcagc tcttccctcc aggggagttt cagaaagata tccggtgggg ggaaggggat
     4021 gtggaggaag aagacgcatt tcctctttac ccccgaagat cccagcattc tggcgacggg
     4081 gatcatctgg tagtcgtgga cccagacaaa gtcctcggcc ctggtatacc ttgagacagc
     4141 cctggcgaaa aggccgttga ccctcctgta ggacttccag tactccggtt cgaaacggca
     4201 ccgggtctga agatcatgga agaggggcca gacaacctcg tttgagaatc cgtagtagaa
     4261 ggaatctaca tcttcccttg acagcctgac agggaagacc
//
LOCUS       DAOPKQ010000115         4266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_22701_length_4266_cov_4.856804, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000115 DAOPKQ010000000
VERSION     DAOPKQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22701_length_4266_cov_4.856804"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PK146_04915"
     CDS             complement(<1..894)
                     /locus_tag="PK146_04915"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPE91616.1"
                     /translation="MRTEVFAVDGVEPEKDVISRAAGVIRRGGLVAFPTETVYGLGAD
                     ALSPKAVRRIFEAKGRPVDNPLIVHVGERDAADRLGQVDERSRRLMDLFWPGPLTLVI
                     RAAREVPAEVTGGLATVAVRMPAHPVAIALIKGSGIPIAAPSANRSGRPSPTTAEAVL
                     EDLGGRVEIILDAGPTVVGVESTVLDVTGDLPVLLRPGGVSLEELTRAVGPVLPSPGG
                     IHDAGSPGTRYRHYAPSIPVVLRGPGEEWAKDPRLCRSGVRIAYVGMSPSPVVALIEI
                     RFGTVEGYATGLFSALRELERS"
     gene            complement(891..2012)
                     /gene="tgt"
                     /locus_tag="PK146_04920"
     CDS             complement(891..2012)
                     /gene="tgt"
                     /locus_tag="PK146_04920"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPE91617.1"
                     /translation="MFGFRIDSRCPDTMARMGELRTDRGSVPTPVFMPVGTRATVKAM
                     APFELQEMDARIILANTYHLYLRPGHEVIREAGGLHRFMAWPGLILTDSGGFQVFSLS
                     TLRKISDDGVTFRSHLDGTTHLMTPELSIKVQEALGSDMAMCFDECVPYPCSPEDSQE
                     AVRRTLLWARRCQDVHSRADQALFGIVQGSVFEDQRISCARELAEMDFPGYAVGGLSV
                     GEPHGEMYRILDAVTPALPEGKPRYLMGVGYPPNLVEGVARGIDMFDCVLPTRNGRNG
                     TLFTPGGRMNIKNLEYERDFRPIDPQCGCYACRNFTRAYIRHLFRSGEILASRLCTWH
                     NLHYLLDLMKGIRGSIRSGTFADFRKRFHEEFREGGERG"
     gene            complement(2012..2797)
                     /locus_tag="PK146_04925"
     CDS             complement(2012..2797)
                     /locus_tag="PK146_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPE91618.1"
                     /translation="MPRFVDTHCHLAMKDFSEDLPEVMDRAAEAGVERILVVGSDEAG
                     NIDAFGLVKRSGAGRLFTAVGIHPHESSSASSGIPGTVAEMVRDPKVVAVGETGLDFF
                     YDHSPRDVQERVFSEHVALARKVKKPLVVHVRDAYGEALKILRREKADECGGVIHCFS
                     GSLEDAIAAIDMGFFISFAGPLTYPGNGPLRETAAVLPLERLLCETDAPYLSPQPKRG
                     RRNEPANVAYVYQTLAQIRGLTLEVCAANISLNASRLFRWGAE"
     gene            complement(2869..>4266)
                     /gene="metG"
                     /locus_tag="PK146_04930"
     CDS             complement(2869..>4266)
                     /gene="metG"
                     /locus_tag="PK146_04930"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HPE91619.1"
                     /translation="PGAILPQSRYNEVLSFIRSGLRDQSISRTSVSWGVPLPGDERHV
                     IYVWFDALINYLTVCGYPHNGELVEKYWPTVNHIMAKDIIRFHCIVWPAMLLALGLNP
                     PATVFSHGWWTVEGEKMSKSRGNVVDPFEMAALYGVDAFRYFLMREIPFGSDGDFSER
                     ALVGRINSDLANDQGNLLNRTLQMIDNFTGGVVPFNSGTVEDLDREIRTMALATLDSY
                     RARMDSYAFDEALKEIWTLIRRANKYIDETMPWKLGKEGNLERLGPVLNTLCETLRFT
                     AAMVSPFMPGTARKIFDQLGLSGDPTELLLDELEWGKIPEGCRVSKKAVLFPRIDLKE
                     WEKKKAERDARKGATPDPGDHEDEVSIDDFKKIELRVARVVKVEAVPKADKLYRMDLD
                     LGYERRTIVSGIREFRAPEDLEGRQIIVICNLKPASLRGVVSNGMLLAAETSDGKSLA
                     LLTVDQEIAPGSRVH"
BASE COUNT          796 a         1366 c         1240 g          864 t
ORIGIN      
        1 ggagcgttca agctccctca gcgccgaaaa gagcccggtt gcatacccct cgacggtccc
       61 gaagcggatc tcgatcaggg cgaccaccgg tgaaggggac attcccacgt aggcgatcct
      121 gacgccactt cggcaaagcc tggggtcctt cgcccactcc tcgccggggc ccctcagtac
      181 caccggtatt gacggagcgt agtgccgata ccttgtcccg ggcgaccccg cgtcatgaat
      241 gcctccaggg gacgggagga cagggcctac agcccttgtc agctcctcca gtgagacacc
      301 cccggggcgc agcagtacgg ggagatcccc agtgacgtcc agcaccgtgg actcgactcc
      361 gacgactgtt ggtcctgcgt ccaggataat ctctaccctt cccccgaggt cctcaaggac
      421 tgcttccgcc gtggtcgggc tcggccttcc gctcctgttg gcgctagggg ccgcgatagg
      481 gatcccggat ccttttataa gcgctatagc caccggatga gcgggcatcc tgaccgccac
      541 agttgcaaga cccccggtga cctcggccgg cacttctctt gctgccctga tgacgagggt
      601 cagcggtccc ggccagaaga gatccattag ccttcgggac cgttcatcca cctggccgag
      661 cctgtccgcg gcatcccttt ccccgacgtg gacgataagg gggttgtcta caggcctccc
      721 ctttgcctcg aatatcctcc tcacagcttt cggggacagg gcatcggccc caaggccgta
      781 aacggtctcc gtcggaaagg ctacgagacc gccacggcgt atgacccccg cggctctgct
      841 gatgacatcc ttttcgggtt ccacaccgtc gacggcaaag acctctgtcc tcaacccctt
      901 tcccctccct cccggaattc ctcgtggaac ctctttctga aatcagcgaa ggtgcccgac
      961 cggatcgatc ccctgatgcc cttcatcagg tccagaaggt agtggaggtt gtgccaggtg
     1021 cagagccggg aggccaggat ctcaccggac cggaagaggt gacggatata ggccctggtg
     1081 aagttgcggc aggcgtagca tccgcattgg gggtcaatgg gcctgaaatc cctctcgtac
     1141 tcgaggttct tgatgttcat cctcccccct ggggtgaaga gcgtcccgtt ccttccgttc
     1201 ctggtgggca gaacgcagtc gaacatatct attcccctcg cgacgccttc gaccagattg
     1261 ggcgggtaac ccacccccat caggtatctg ggcttccctt cgggaagtgc cggggtcact
     1321 gcatcgagga ttctgtacat ctccccgtgg ggctcgccca cggacaaccc gcccacagcg
     1381 tacccgggaa agtccatttc ggccagttcc ctggcgcagc tgatccgctg gtcctcgaat
     1441 accgaaccct gtactatccc gaagagggcc tggtccgccc tcgagtggac atcctgacac
     1501 ctccgggccc agagcagggt cctcctgacg gcctcctgcg aatcttcagg tgagcagggg
     1561 taggggacgc actcgtcaaa gcacatggcc atatcgctgc cgagagcctc ctgcaccttt
     1621 atggagagct ccggggtcat caggtgggtg gttccatcca ggtgggaccg gaaggtcacg
     1681 ccgtcatcgg atatcttcct cagcgtggag agggagaaca cctggaaacc tccgctgtcg
     1741 gtgaggataa ggccgggcca cgccatgaag cggtgaagtc cgccagcctc ccttatgacc
     1801 tcgtggccgg ggcgaaggta gaggtgatag gtgttggcaa gtatgatcct tgcatccatc
     1861 tcctggagct cgaagggggc cattgctttc accgtcgccc tggtacctac gggcatgaag
     1921 accggggtgg ggacagaccc cctgtcggtt cttagctcgc ccatcctggc catggtgtcg
     1981 gggcaccgtg agtcgatccg gaaaccgaac atcactccgc cccccagcgg aagagcctcg
     2041 aggcgttcaa gctgatatta gctgcgcaga cttccaaagt caggcccctg atctgggcca
     2101 gggtctggta gacataggct acattggccg gctcattgcg cctgcctctt ttgggctggg
     2161 gcgacaggta gggcgcgtcg gtctcgcaca gcagcctctc cagggggaga accgccgccg
     2221 tctctctcag aggaccattc ccggggtagg tcaagggtcc ggcaaaggag atgaaaaagc
     2281 ccatatcgat ggcggcaatg gcatcttcca ggcttccgga aaaacagtgg atcacgcccc
     2341 cgcattcatc ggccttctct ctccggagta tcttcagggc ctccccgtag gcgtccctga
     2401 catggaccac caggggtttt ttcaccttcc tcgcaagggc tacgtgctcc gagaagaccc
     2461 tctcctgaac gtcccttggc gagtgatcgt agaagaaatc aagccctgtc tcgcccacgg
     2521 ccactacctt ggggtcccgg accatctccg ctaccgttcc ggggatcccg gaggaggcgg
     2581 aggatgattc gtgggggtgg atacccaccg ccgtgaagag ccggcccgcc ccggaccgct
     2641 tcaccagtcc gaaggcatcg atattgcctg cctcgtcgga accgacgacc aggatcctct
     2701 caactccggc ttctgcggcc ctatccatca cctccggaag gtcttcggag aaatccttca
     2761 tggcaagatg acagtgtgtg tcaacgaacc ttggcattgg caacctcctc caaaatcaaa
     2821 aggggccgga gatccccggc cccaaaaagc acgccttcat gcgctgggtc aatggaccct
     2881 tgagccgggg gcgatctcct ggtcgacggt caaaagagcc aggctcttgc cgtcggacgt
     2941 ctcagcggcc aaaagcatcc cgttgctgac caccccgcga agagaggccg gtttaaggtt
     3001 gcagatcact attatctgcc tcccttcgag atcctcgggg gcacggaact ccctgatccc
     3061 cgagacgatg gtccttctct cgtaaccgag gtccaggtcc atcctgtaga gcttgtcggc
     3121 cttgggcaca gcctcgacct tgacgacccg ggctacgcgg agctcgatct tcttgaagtc
     3181 atcgatggag acctcatcct catgatcccc cgggtcggga gtggctccct tcctggcgtc
     3241 cctctctgcc ttcttcttct cccactcctt gagatctatt ctggggaaga gaactgcctt
     3301 cttgctgacc ctgcagcctt caggtatctt cccccactcc agttcatcaa gcaggagttc
     3361 cgtcggatca ccggagaggc ccaactggtc gaagatcttc ctggcggtcc cgggcatgaa
     3421 gggcgatacc atggccgctg tgaacctgag ggtctcgcag agggtgttaa ggacaggtcc
     3481 aagcctctcg aggttccctt cctttcctaa tttccagggc atggtctcgt cgatgtactt
     3541 gttggccctt ctgataagcg tccagatctc cttcagggcc tcgtcgaagg cgtatgaatc
     3601 catcctggcc cggtaggaat cgagcgtggc cagggccata gtcctgatct ccctgtccag
     3661 gtcctccacg gtgccggagt tgaagggcac gacccccccg gtgaagttgt ctatcatctg
     3721 cagggtcctg ttgaggaggt tgccctggtc attggccagg tcagagttga tgcgccccac
     3781 cagcgccctc tcagagaaat cgccgtcgga gccgaagggg atctccctca tgaggaagta
     3841 cctgaaggca tctacgccgt acagggcagc catctcgaag gggtccacca cgttgcccct
     3901 ggatttggac atcttctcgc cctccacggt ccaccacccg tgagagaaga ccgtggcagg
     3961 aggattcaaa ccgagggcca ggagcatggc cggccagaca atgcagtgga acctgattat
     4021 gtccttggcc atgatgtggt tcaccgtggg ccagtacttc tccaccagct ccccgttgtg
     4081 ggggtatccg catacggtga ggtagttgat cagggcgtcg aaccagacgt atatcacgtg
     4141 cctctcatca ccgggtaggg ggacccccca ggagacggag gtccttgaga ttgactggtc
     4201 cctcagtccg cttcttatga agctaaggac ctcgttgtag cgtgactggg gcaggatcgc
     4261 cccggg
//
LOCUS       DAOPKQ010000116         4226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_22981_length_4226_cov_5.772716, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000116 DAOPKQ010000000
VERSION     DAOPKQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22981_length_4226_cov_5.772716"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK146_04935"
     CDS             <1..453
                     /locus_tag="PK146_04935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPE91620.1"
                     /translation="DLFNRQHFDEVLQREIDRSSRYGTPLSLIMIDLDNFKSLNDTKG
                     HIAGDRALSGIARTISENIRSSDVAARWGGDEFILATPADTEQALSLARKLKGLLLGL
                     DHEGYGPVTGSIGVSTFREGDDIDSLTKRADRAMYDVKKKGGNGVSSR"
     gene            complement(621..953)
                     /locus_tag="PK146_04940"
     CDS             complement(621..953)
                     /locus_tag="PK146_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPE91621.1"
                     /translation="MSVSGNLFSLPETLPEEEVFEDLLPDSPVRIERIISTGQATPDG
                     IWLKQEMDEWVVLLQGQAVLEYEDGRRSDLDRGDWILIPASTRHRVVLTSSFPPCVWL
                     AIHGKLLE"
     gene            complement(958..2910)
                     /locus_tag="PK146_04945"
     CDS             complement(958..2910)
                     /locus_tag="PK146_04945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE91622.1"
                     /translation="MSSVDRARTVEEACRAAVSAIADYTRFSNPSLFLVDPEGQNLVL
                     VAHVGFTPGTLAIPRGRGVSWISLETGKSALIDDVTLENDYLPGLEGSRCELSVPVIW
                     GDRKIGVFSIESRVPGAFTTDDARFTNLLAAILGSVIVHLETETRLSESLKDLEMTAR
                     YRSLFLELFFELFSMRERDVFLDRVVDILGEVMRYDKIYLFLRQTRSGPLWLRAFRGK
                     NIGEKAIRDIIEKGMGITGRAIRTGVAVFCNDTSKDPDFYLDDTRTRSEAALPIRFGD
                     TLWGVLVVDEYRPDAFSPSDREQLTILAGMIGVVLENIHQFRRLREELSLMEVLHRII
                     AVVAGERDLRGLCHETVRLLRENTRYHFVEIFEVVSGITGEVRIIGGGSEEDEFYREQ
                     EELLREREWGLVGLAARTGRLVNVPDVSNSPQYVPIKRTTRSELDVPIEFGGNLYGIL
                     SLESDIPGAFTEDDESAMSILSGHLGVLWAHRELLERTERQAMQDPLTGLWNRRFIFG
                     RLDAEIARCKRYGCRFSIAMLDLVNFKAVNDRFGHTTGDRVLVDTGSFFACSLRACDM
                     VARYGGDEFLVLLPETDLDEAMCIMERLLERYRGLTFCGGEAQVSFDFGIASFPADGR
                     DRGSLVRMADENLYSQRGIITRPEGG"
     gene            3092..3724
                     /locus_tag="PK146_04950"
     CDS             3092..3724
                     /locus_tag="PK146_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPE91623.1"
                     /translation="MLIANRSGISRLVERLSEFPNCNDLASFLRGLEIRDGGNGNGVV
                     LFGDGSVLHYERGVDMQPERAAAILEGRDYEWAVFHSREASVGSVHDENCHPFVIEGK
                     HHLVMAVNGNETHMTALGKLIGDRTDSEFIARMIVDMDLPFPPVLACFNSNFVGLYDG
                     RPFAKKGDRQLLKFQSGRDVVFASDLPFGMLDITKPEQNYCWFDWEESIL"
     gene            3748..>4226
                     /locus_tag="PK146_04955"
     CDS             3748..>4226
                     /locus_tag="PK146_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HPE91624.1"
                     /translation="MESITVYGAGSWGTAVADLLARRGHQTLLWCRRQEQARAINSTG
                     SNPDYLRNVRLCPTLTVTSDLEEAAEASEMHVSAIPTQSIREFLSRAGKIWSRDLRVC
                     NLAKGIEIVSGKRISQIFSEILPGSRYSVLSGPSHAEEVAAGKPTAVVVASSIPGEA"
BASE COUNT          937 a         1229 c         1170 g          890 t
ORIGIN      
        1 gacctgttca accgtcagca cttcgacgag gtcctgcaga gggagatcga caggtcctcg
       61 agatacggga cccccctgtc gctgatcatg atagaccttg acaacttcaa gagccttaac
      121 gacacgaagg ggcacatcgc cggcgacagg gctctctccg ggatagcgcg gactatcagc
      181 gagaatattc gttcatccga tgtcgctgcc cgatggggag gggacgagtt catcctggcc
      241 acccctgccg acacggagca ggctctctcc cttgcccgaa agctcaaggg actgcttctc
      301 ggcctcgatc acgaggggta cggcccggtc accgggagca tcggagtatc aaccttccgc
      361 gagggggacg acatagattc tctcaccaaa agggcagatc gcgccatgta cgatgtgaag
      421 aagaagggcg gcaacggagt gtcttcaagg taggtacccc gactgtccag ccatctccaa
      481 agtagagatc agacgagacc ctggggaccg gtcgccagag agtatcgttc ctggtagaag
      541 gtagaggaca agcgaagatc ttatatcagc gacaagcgga aaggggcggg caagaacccg
      601 cccctccttg ttgctgcagc tcactcaaga agctttccat ggattgccag ccacacacag
      661 ggggggaagg aggaggtcag gacaacacga tgcctggtgg aagccggtat gagtatccag
      721 tcccccctgt ccagatccga cctccggcca tcttcgtact ccaggactgc ctgtccctgc
      781 aggagcacta cccactcgtc catctcctgc ttcagccaga tgccgtcggg cgtagcctgc
      841 cccgtcgata tgatcctctc tatcctgacc ggagagtccg gaaggaggtc ttcgaagacc
      901 tcctcttcgg ggagagtctc cggcagagag aaaagattcc ccgaaacact caaggtatca
      961 accgccttcc ggtctggtga ttattcctct ctggctgtaa agattctcat cggccattcg
     1021 taccagagac ccccggtccc ttccgtcagc cgggaaagag gctataccga aatcaaatga
     1081 gacctgggcc tcccctccgc agaaggtcag acccctgtat ctttccagca gcctttccat
     1141 tatgcacatg gcttcgtcca gatccgtctc gggaagcagg accaggaact cgtcccctcc
     1201 gtatcgagcc accatatcgc aggccctgag agaacatgca aagaaggaac ccgtatctac
     1261 caggaccctg tcccccgtgg tgtggccgaa gcggtcgttt actgccttga agttcacaag
     1321 atctaacatg gctatgctga accggcagcc gtaccttttg catcgtgcga tctccgcgtc
     1381 cagacggcca aagatgaagc ggcggttcca tagacccgtt aaagggtcct gcatggcctg
     1441 cctctccgtc cgctccagga gttcccggtg agcccacagg actccaaggt gacctgaaag
     1501 gatcgacatg gcactctcgt cgtcctcggt gaaagccccg gggatatcgc tctccagtga
     1561 aaggattccg tagagattcc ctccgaactc gataggaacg tcaagctccg agcgagttgt
     1621 tctttttatc gggacgtact gcggggagtt gctcacgtcg gggacgttga caagccggcc
     1681 tgtcctggcg gccagcccga caagccccca ctccctttcc cggaggagtt cttcctgctc
     1741 ccggtagaat tcgtcttctt ctgaccctcc gccaattatc cggacctctc ccgttatccc
     1801 tgacactacc tcgaagatct ctacaaaatg gtacctggtg ttctccctga gaaggcggac
     1861 agtttcgtgg cagagacccc tgagatccct ctctccggcg acgacagcaa ttatcctgtg
     1921 caggacctcc atcagtgata gttcttcacg gagccttctg aactgatgga tgttctcgag
     1981 gaccaccccg atcatgccgg ccaggatggt cagctgttcc ctgtcggagg gggagaaggc
     2041 gtcaggtctg tactcgtcaa ccaccaggac accccagagg gtgtccccga aacgtatggg
     2101 caaagccgcc tctgaccggg tcctggtgtc gtcgaggtag aaatcgggat ccttcgaggt
     2161 gtcgttgcag aagaccgcca cccctgtcct gatggctctt ccagtgatgc ccatgccttt
     2221 ctcgataata tcccggatag ctttttcgcc aatgttcttt cccctgaaag ccctaagcca
     2281 caagggaccc gacctggtct gcctgagaaa gaggtagatc ttgtcatacc tcataacttc
     2341 gccgagtatg tccactaccc tgtccagaaa gacatccctt tccctcatcg agaaaagttc
     2401 gaagaagagc tcaaggaaaa gtgaacggta cctggccgtc atctccaggt ccttcaggct
     2461 ttccgacagg cgggtctcgg tctcgagatg gactataaca ctcccgagaa tggccgccag
     2521 gaggttcgta aaacgggcat cgtcagtggt gaacgcccca ggtactcttg attcgatgct
     2581 gaagacccct atcttgcgat ctccccagat cacagggacg ctgagttcac aacgtgaacc
     2641 ttcaaggccg ggcaggtagt cattttcgag ggtcacatca tcaatgaggg cgcttttccc
     2701 tgtctcgagg gatatccagg agacccctct gccccggggg atggcgagag taccaggggt
     2761 aaagcctacg tgtgccacca gtacaaggtt ctgtccttca ggatcgacca ggaatagtga
     2821 aggattcgag aaacgggtgt aatcggctat ggccgaaaca gcggcccggc aagcctcctc
     2881 cactgtcctg gcccgatcta cgctactcat gtacttgaaa aggacgtcct tttccaatta
     2941 ttgccccccc tgtttttcat tgaactctta ttatatacac tcctcaattt ccttaacaag
     3001 ggaatgacta gcttctattg tcggccttgc tggtatatca tgtttcacaa ggcccgtgag
     3061 gccaccagtg ggggttgaga tcttgtgcag aatgcttata gctaacaggt cagggatctc
     3121 caggctcgtg gaaagacttt cggagttccc gaactgcaat gatctggcct cttttctgag
     3181 ggggctggag atccgcgatg gcgggaacgg gaacggagtg gtactcttcg gcgacggatc
     3241 ggtcctccac tacgagcgag gggttgacat gcaacccgaa cgggctgcgg ccatccttga
     3301 gggacgtgac tacgagtggg ccgtatttca ctcaagagag gcaagcgtgg gatccgtcca
     3361 cgacgagaac tgccacccct tcgtgatcga ggggaagcac cacctcgtta tggcggtgaa
     3421 cggtaacgag acccatatga ccgccctcgg gaaattgatc ggcgacagga ccgacagcga
     3481 attcatcgca aggatgatcg tggatatgga tctgcccttt cccccggtac tggcctgctt
     3541 caattccaac tttgtaggcc tttacgacgg ccgtccattt gccaagaagg gtgatcggca
     3601 gctcctgaag tttcagtcgg gacgggacgt tgtcttcgcc agcgacctgc ccttcggaat
     3661 gttggacata accaagccgg aacagaacta ctgctggttc gactgggaag agtcgatcct
     3721 gtagggctgg atccgggagg aggcataatg gagagcataa ccgtctacgg agcaggaagc
     3781 tggggtaccg ccgttgccga cctgctcgcc cgcaggggac atcagaccct tctctggtgc
     3841 aggagacaag agcaggcgcg agctatcaat tccacgggta gtaatccgga ctacctgagg
     3901 aatgtccggc tttgccctac cctgacagtg acctctgatc tggaagaggc ggcagaagct
     3961 tctgaaatgc acgtctctgc cattccgacc caatccataa gggaattcct gtcaagagcc
     4021 ggaaagatct ggtcccggga cctgagggta tgcaacctgg cgaagggtat agagatcgtg
     4081 tccgggaaga ggataagcca gatcttttcc gagatccttc ccggttcgag gtattcggtg
     4141 ctctctggtc ccagccatgc ggaagaggta gccgccggca aacccaccgc cgtcgtggta
     4201 gcctcttcga tcccagggga agcgga
//
LOCUS       DAOPKQ010000117         4186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_23309_length_4186_cov_4.968773, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000117 DAOPKQ010000000
VERSION     DAOPKQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23309_length_4186_cov_4.968773"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PK146_04960"
     CDS             <1..509
                     /locus_tag="PK146_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPE91625.1"
                     /translation="SAAVLAVKGYPMNAIGADQRDPRITNLVVSLRSACGVKTMSKGL
                     DLKGAIRCLRDGEVLGVLLDQDFGDNGTVVPFMGIPASTPFGPVKMADRIGSSVVPMF
                     IVRRPDGIHHDLYIQPPLGEAGGLPFGKDLEASLELCNDTLSEWITRYPGHWMWLYPR
                     WASTTGDR"
     gene            511..999
                     /locus_tag="PK146_04965"
     CDS             511..999
                     /locus_tag="PK146_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPE91626.1"
                     /translation="MLIAMDPGTEKFGWAVSSDTGDLLLSGVLPIDEIDEWAKAVLRG
                     DTSFLEERAVERSAARPDRSFPGFVIAGSGTGSAKCIRRLASSGLRVEKVPESFSTLR
                     GRDLYWRIHPPRGFHRLVPKGLLVPPRDVDDLAAWSLILEFIGGNKEMPCNEVREGMQ
                     DG"
     gene            992..1348
                     /locus_tag="PK146_04970"
     CDS             992..1348
                     /locus_tag="PK146_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91627.1"
                     /translation="MAEKARLKVFIEQPGFSVGCVDLDYGEEEMKSFPIAEDRISVGS
                     LLVRLRERYGDELRIDLIDPRNIAYLLDVLRFRVNNTEAVWVLDGKVIFRGIPEWCVL
                     MNEVDQITGKRSENRE"
     gene            1348..2772
                     /locus_tag="PK146_04975"
     CDS             1348..2772
                     /locus_tag="PK146_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPE91628.1"
                     /translation="MRRWLRSIAEEELVLGYAFIKWVVLSVVAGIVVGVAAAAFLLLL
                     EKSIVAANTLGRYRVLLLPLGLLASVLIVRKLAPEAAGHGTEKVIEAVHQRAGRISLP
                     TVPVKLVATIITIATGGSAGKEGPCAQIGAGLTSAMAGLLRLNEVDRKKLVVCGISAG
                     FSAIFGTPVSGAIFGLEVLYIGQIYYDVMLPAFISGIVAFQTAVHLGVHYADQSLAVF
                     PALASSSIIWVALSGVFFGLVALIHIEIVTVTEKYLKSIPARPEVKAFGSGLVLLILS
                     LFFGTRYLGLGTGFIEAAVSGEPVPGGAFLVKSLFTGITLASGGTGGILTPTFFVGAG
                     AGSFFATLFALDLPLFSSIGFVAVLSGAANTPIAATIMAIELFGGGIASFAAIACIIS
                     FAISGHRSIYPSQILARPKSPVFRFSDEDVTVENHRKKINLSITIIPELLSGLRDCWD
                     REVLFPRDEERGPGEENLPPQDGE"
     gene            complement(2757..3461)
                     /locus_tag="PK146_04980"
     CDS             complement(2757..3461)
                     /locus_tag="PK146_04980"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPE91629.1"
                     /translation="MSRKPHKVLGVLGGMGPAATADFLRILTEMAGVERDQDHPKVLL
                     FSNPQIPDRTAAITGGGGDPAPLILEGLQRLESWGAQLLAVPCNTAHVFIDRFREKLK
                     VPLVHIVEETLSRARIHSPSGAWLTATSGTTASGIYSREAERQGYELLLPGEDVQKEL
                     QEIVSLVKANRLDKSGRAMAETVRRLQRERDLPVVAACTELPLAYQASGLEPSGMVSS
                     LGSLAEACLERIYSPS"
     gene            complement(3442..>4186)
                     /locus_tag="PK146_04985"
     CDS             complement(3442..>4186)
                     /locus_tag="PK146_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPE91630.1"
                     /translation="AGEKGKILINVMDSVAETMYKMTGLIMQLAPYGVFALISTTVAK
                     YGISVLAPFGKVIFAVYLGCILHALIVYSGLLTVWARFSPWRFFDGVKEATLTAFVTR
                     SSSATLPITMRVTQENIGVSEKISSFVLPLGATINMDGTALYQGVCALFVAQAFGIEL
                     SLGAQLGILLTATLASIGTAGVPGAGLIMLTLVLTQAGLPVEGVALLAGIDAVLDMAR
                     TAINVTGDATVATVVAKTEDELADVKKAA"
BASE COUNT          848 a         1136 c         1258 g          944 t
ORIGIN      
        1 actcggctgc agtgcttgcc gtaaaaggct atcccatgaa cgccatagga gccgatcaga
       61 gggatcccag gataacgaac ctggtcgtat cactccggtc agcctgcgga gtgaagacga
      121 tgagcaaggg gttggacctg aagggggcta tccgctgcct cagggatggt gaagtactgg
      181 gcgtactcct ggatcaggat ttcggcgata acggaacagt ggtccctttc atgggcattc
      241 cggccagcac tccatttggt ccagtcaaaa tggcggacag gataggatcg tcagtggttc
      301 ctatgttcat agtcaggcgt cctgatggta ttcaccacga tctttacatc cagccccctc
      361 tcggcgaagc gggggggctt cctttcggga aagacctgga ggcatccctg gagctttgta
      421 acgacacact gagcgaatgg atcacccgtt accccggaca ttggatgtgg ctctacccga
      481 gatgggcttc aacgacgggg gaccgttgag atgctgatag caatggaccc ggggacggaa
      541 aagttcggtt gggctgtctc aagtgatacg ggagacctgc ttctctcggg agttttgccg
      601 atcgatgaga tcgatgaatg ggcaaaagcg gttttgcgcg gggacacaag tttccttgag
      661 gagagggctg ttgaaagaag cgccgctcga cctgacaggt cctttcctgg tttcgtgata
      721 gcaggttcag gtacgggaag cgcaaaatgc atcaggagac ttgcctcttc aggtctcagg
      781 gttgaaaagg ttccggagag cttctctacg ctcaggggca gggatctcta ctggcggatc
      841 catcctccca gggggtttca caggcttgtc ccgaaaggct tgcttgttcc cccaagggac
      901 gttgatgacc ttgccgcctg gagccttatt cttgaattca tcggcggcaa caaggaaatg
      961 ccgtgtaatg aggtgcgaga aggtatgcag gatggctgag aaggcaaggc tgaaagtttt
     1021 tatcgaacaa ccgggttttt cggtcggctg cgtcgacctg gattacggcg aggaagagat
     1081 gaagagcttc cctattgccg aagacaggat cagcgtcggg tccctgcttg tcaggctcag
     1141 ggagagatac ggcgacgaac tcaggatcga cctgatcgat ccccgaaaca tagcctacct
     1201 cctggacgtc ctccgtttcc gtgtgaacaa cacagaggct gtttgggttc ttgacggaaa
     1261 ggtgatcttc cgggggatcc ccgaatggtg tgtcctcatg aatgaagtag accagataac
     1321 gggtaaaagg agtgagaacc gggaataatg aggcgctggt taaggagcat cgctgaggaa
     1381 gagcttgtac ttggatacgc cttcatcaag tgggtcgtat tatcggtcgt tgcggggata
     1441 gtggttggtg tggctgcagc tgccttcctt ctgctccttg aaaagagcat tgtcgctgcc
     1501 aataccctgg gcagatacag ggttctgctt ttgcccttgg ggcttctggc aagcgtcctg
     1561 atagtcagga aactggcccc cgaggctgca ggtcacggta cagaaaaggt gatcgaggca
     1621 gtccaccaga gggcagggcg gatatccctc ccaacggtac cggtgaaact ggtcgccacg
     1681 atcataacca ttgcgacagg tggatctgcg ggcaaggagg ggccctgtgc ccagataggt
     1741 gcgggactta cctcggccat ggcaggtctc ttgagactta acgaggtgga caggaaaaaa
     1801 ctggtggtct gcggcatatc ggcaggattc tcggctatat tcggtacacc cgtttctggt
     1861 gccattttcg ggcttgaggt cctctacata ggccagatct attatgatgt aatgctaccg
     1921 gccttcatca gcggcatcgt tgctttccag accgctgttc acctcggggt ccattacgct
     1981 gaccagagcc tggccgtctt cccggcgctg gccagttcca gcattatatg ggtggccctc
     2041 tcgggtgtct ttttcggtct tgtagccttg atacacatag agatcgtcac tgtgaccgag
     2101 aagtacctga agagcatacc cgccaggccg gaggtaaagg ctttcggcag cgggttggtg
     2161 ctcctgatcc tctccctctt cttcggaacc cgctacctcg gcctggggac gggtttcata
     2221 gaagcggccg tttccggaga gcctgtcccc gggggagcct ttcttgtcaa atcccttttc
     2281 accgggataa ccctggcatc gggcgggacg ggggggatac ttactcctac gttcttcgtt
     2341 ggcgccggtg cgggttcctt ctttgccacg ctctttgccc tcgaccttcc gctcttcagc
     2401 tccatcggat tcgtagcggt gctttcgggg gccgccaata cccccatcgc ggccacgata
     2461 atggccattg agctcttcgg gggtggaatt gcctcttttg cggccattgc ctgcatcatc
     2521 tccttcgcca taagcggcca ccggagcatc tatcccagcc agatccttgc aaggccaaaa
     2581 tcccccgtgt tcaggttctc tgacgaagat gtcaccgtcg aaaaccatag gaagaagatc
     2641 aacctttcga taacgatcat cccggagctt ctctccggtc tgagggactg ctgggatcgg
     2701 gaagttctct tccctcggga cgaagagagg gggcccggcg aggagaacct ccctcctcag
     2761 gacggggagt agatcctttc caggcaggct tcggcaaggg agcccaggct tgagaccatt
     2821 ccggaaggtt ccagccctga agcctggtat gcaaggggga gctccgtaca ggccgccact
     2881 acggggaggt ccctttctct ttgcagcctc ctgaccgttt cagccattgc ccttccggac
     2941 ttgtccagcc tgtttgcctt gaccagggat actatctctt gcagttcctt ctggacatcc
     3001 tcgccgggaa ggaggagttc gtagccttgt ctctcggctt cgcggctgta tatccctgaa
     3061 gcggtggtac cgctggtagc agtgagccag gcccctgatg ggctatgaat ccgcgctctg
     3121 gaaagggtct cctcgactat gtgaacgagg ggtaccttaa gtttttcgcg aaatctgtcg
     3181 atgaatacat gggccgtgtt gcaggggacc gccagaagct gggcacccca tgattcgagc
     3241 ctctgtagcc cctccaggat aaggggggcg gggtctcccc cgccccctgt tatcgccgcg
     3301 gtcctgtcgg ggatctgggg gttcgagaag agaaggactt tgggatggtc ctggtctctt
     3361 tcaactccgg ccatttccgt cagaatacga aggaaatccg ctgtggcggc cggccccatt
     3421 cctccgagaa ccccaaggac cttatgcggc tttcttgaca tctgccagtt cgtcctcagt
     3481 tttcgctacg accgttgcga ccgtggcgtc tccggtgacg ttgatggccg tccgggccat
     3541 gtcgagaacc gcgtcgatcc cggccagaag agctacccct tcaacgggga gccctgcctg
     3601 ggtgaggact agggtcagca ttatcagacc cgctccggga acccccgccg ttccgattga
     3661 ggcgagggtt gcggtaagga gtatccccag ctgtgcgccc agggagagtt ctatcccgaa
     3721 ggcctgggcg acgaacaggg cacagactcc ctggtagaga gccgtgccgt ccatgtttat
     3781 cgtggcgccc agaggaagaa cgaaggagga tatcttctcc gagactccta tgttctcctg
     3841 ggtcaccctc atggtgatag gaagtgttgc tgaactggaa cgggtcacga acgccgtcag
     3901 cgttgcctct tttacgccgt caaagaaacg ccatggcgag aacctggccc acaccgtgag
     3961 aagccccgag tagacgatga gtgcgtgtag aatacatcca aggtagacgg caaagatcac
     4021 cttgccgaag ggcgccagca cagagatccc gtacttagcg accgtggttg atataagtgc
     4081 gaacacgccg tagggggcca gctgcatgat caggccggtc atcttgtaca tggtctcagc
     4141 gacggagtcc attacgttga tcaaaatctt gcccttctcc ccggca
//
LOCUS       DAOPKQ010000118         4184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_23325_length_4184_cov_5.815936, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000118 DAOPKQ010000000
VERSION     DAOPKQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23325_length_4184_cov_5.815936"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..458
                     /locus_tag="PK146_04990"
     CDS             147..458
                     /locus_tag="PK146_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE91631.1"
                     /translation="MERPLVVKFKRPDSIRDYPCGAFRVGTLVQENSGWRVFRPVIDT
                     GKCIKCLRCFLVCPDGVIDKSGEDLEIDYRFCKGCGVCAYECKVGAIVMMREGDDGNA
                     R"
     gene            448..1626
                     /gene="porA"
                     /locus_tag="PK146_04995"
     CDS             448..1626
                     /gene="porA"
                     /locus_tag="PK146_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HPE91632.1"
                     /translation="MPGRKVFISGDEAVAEAVRLARPNVISAYPITPQTVTVERISEF
                     VEDGSLKAEYLHVESEHSAMSAALGAAAVGARTFTATSSQGLLYMAETLPYVSGARFP
                     IVMMNANRTLATPWALLSDHRDSLSQLDSGWIQVYCENAQEAMDMIIQAYRIAEDPRV
                     MTPFMVNLDGFILTHTYEMVDLPAQENVDRFLPPYRTANRMDLDDPKTVCYAALDDYM
                     EFHLKQELDMRKVPGVIEEVELEFKGIFGRSYGGLVEKYCCEDAEAVLMTLGSVTGTA
                     RAVVDAMRMEGKMVGLAKIRFMRPFPAAEIAGIAGNTKAMGILDRDLSVGYEGIVFTN
                     ARSAVCEIESPPRMINFIGGLGGRNISKEDMRQIFDQLMQAARGEQGERVRFVNARCE
                     "
     gene            1628..2530
                     /locus_tag="PK146_05000"
     CDS             1628..2530
                     /locus_tag="PK146_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE91633.1"
                     /translation="MDEKMVTNAGDLTNTEFFYGHRACAGCGGSLALRLAMKVLGPRI
                     FVAIPANCMSATSFLYPQVSFSVNAMISPFPATGAVLSGIEAGARALGIRDHHVVGFA
                     GDGGTADIGIQALSGAIDRGDRIIYVCYDNEAYMNTGIQKSGLTPYGSRTTTTPAGKN
                     IPGAVGRKKDMFGIVAAHGIKYAATASIGYPEDYLRKISKAAMCKTTSYIHVLAPCST
                     GWGFPAEETVDIGREAVDCGLWYLAEYEKGDFILNRKPKEFSSISEYLKRQARFRHLG
                     EKEIEEITVGRDEKWKKIMKNWRV"
     gene            2849..3736
                     /locus_tag="PK146_05005"
     CDS             2849..3736
                     /locus_tag="PK146_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPE91634.1"
                     /translation="MNKNKHHSVLERITRFSNSLGGQQRALARFILQNPETAAFMNSV
                     QLSEAAKVSNPTVTRLAIKLGFKGFPEFQEALQETVRAQIRSIRRFMEDPGVEEEPLF
                     QRVLSLEFHVLGEMKRRLKEETLQRAVDLISEKRRIFIVGFLANICHAEYMAYFLGTL
                     REGVHLLTRAGDNLFGHLQNTGPEDLAIIYSFPRYPSSTQKLALYLRSKDVPIIGVSD
                     TVLSPLASCSDILLEAPMKFLSFIDPCAGAFALNHCLLTAFYFRYPEKMREALRMFEE
                     YSHRQNLFLMEDIDIVDLV"
     gene            3764..>4184
                     /locus_tag="PK146_05010"
     CDS             3764..>4184
                     /locus_tag="PK146_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HPE91635.1"
                     /translation="MEVMLISQKEIESLRIPVTEVMDVVEKGFALKGEEKMEMPAKVG
                     IHPRKDCFIHAMPCYAGGSADVAGIKWVSGYPINQAKGLPYITGIMCLNDPDTGFVKV
                     LMDANWITAWRTGAATGVCARHMADPDSEVLAVVGLGV"
BASE COUNT         1004 a         1086 c         1193 g          901 t
ORIGIN      
        1 gctgggtgcc cttgtcgcag tctccggggt ggtgtcgctg tcatcggtcc agaaggccct
       61 gaggcagagc atggacccgt cgatggcgga aaagaactgc acactggtgg cgaaggccta
      121 cgaactcaag aaggaggatc gcttccatgg aaagaccact ggtagttaag ttcaaaagac
      181 ctgacagtat cagggactac ccctgcggag ccttcagggt gggtaccctt gttcaggaga
      241 attccggctg gagggtgttc agaccggtaa ttgacacggg aaagtgcata aaatgcctcc
      301 gttgctttct ggtctgcccc gatggtgtca ttgacaagtc tggagaggac ctggagatcg
      361 actatcgctt ctgcaagggc tgcggtgtgt gtgcctatga atgcaaggtg ggggcgatag
      421 ttatgatgcg ggaaggcgat gatgggaatg cccggtagaa aagtgttcat atccggcgac
      481 gaagctgttg ctgaagctgt ccgccttgcc agaccgaatg ttatctcggc ctatccaatt
      541 acaccccaga cagtgacggt ggagaggatc tcggaatttg tagaggatgg ctccctcaag
      601 gccgagtacc ttcacgtcga atcggagcac agcgccatgt cggcagctct tggagctgcc
      661 gcggtcggcg caaggacctt caccgccacg tcatcccagg gacttctcta catggcggag
      721 acccttccct atgtaagcgg cgcccgattc cccatagtca tgatgaacgc gaacaggacc
      781 ctggcgaccc catgggctct tctctcggat cacagggatt ccctctcgca gctggacagc
      841 ggctggatac aggtctactg cgagaatgcc caggaggcca tggatatgat cattcaggcc
      901 taccgcatcg ctgaagatcc aagggtcatg acacctttca tggtgaacct tgacggcttt
      961 atccttaccc atacctacga gatggtcgat cttccggccc aggagaacgt ggacagattc
     1021 cttcccccct acaggactgc caacaggatg gacctggatg atcccaagac ggtatgttat
     1081 gccgcactgg atgactacat ggagttccat ctaaagcagg aactggacat gcggaaggtg
     1141 ccaggagtca tcgaggaagt ggagctggag ttcaaaggaa tattcggcag atcctacgga
     1201 ggcctggtgg agaagtactg ctgcgaagat gccgaagcgg tcctgatgac cctggggagt
     1261 gtcactggta ccgccagggc agtggtggat gcgatgagga tggaggggaa gatggttggt
     1321 cttgcgaaga tccgtttcat gcgtcccttc ccagctgctg aaattgccgg tatcgcaggc
     1381 aataccaagg ccatggggat ccttgatcgt gacctttcag ttggatacga aggtatagtt
     1441 tttacgaacg caaggtcggc cgtctgtgaa atcgaaagtc ccccgaggat gatcaacttc
     1501 atcggaggtt tgggcggcag gaacatctcc aaggaagaca tgagacagat attcgatcaa
     1561 ctgatgcagg cggcccgggg agaacaggga gagagggtcc gtttcgtgaa cgcgaggtgt
     1621 gagtgatatg gacgagaaaa tggttactaa cgccggggat ctgaccaaca ccgaattctt
     1681 ttacggtcac agggcatgtg cgggctgcgg cggcagcctt gcccttcgcc ttgccatgaa
     1741 ggtcctgggg ccaaggatct tcgttgccat ccctgccaac tgcatgtcgg ccaccagctt
     1801 cctctatccc caggtctctt tctctgtcaa cgcaatgatc agtccattcc ctgcgacagg
     1861 agccgtgctc tccggcatag aggccggagc gcgggccctt gggatcaggg atcaccatgt
     1921 tgtaggcttc gccggtgacg gagggacggc cgacataggg atccaggctc tttccggtgc
     1981 tattgaccgc ggcgacagga tcatctacgt ctgttacgac aatgaagcct acatgaacac
     2041 ggggatccag aagagcggtc tcactcccta tggttccagg acgactacca cgcccgccgg
     2101 gaagaacatt cccggggccg tgggaaggaa aaaggatatg ttcgggatag tcgccgctca
     2161 cgggatcaag tacgctgcaa ctgcgagtat cggttacccc gaggattacc tcaggaagat
     2221 cagcaaggct gccatgtgca agaccacttc ctacatccat gttcttgcgc cctgttccac
     2281 aggctgggga tttcccgctg aagagaccgt cgatattggc agggaggctg tcgactgcgg
     2341 cctttggtat ctggcggagt atgagaaggg cgatttcatc ctgaacagga aaccgaagga
     2401 gttctcatcg atatcagaat acctgaaaag acaggcaagg ttcagacacc tcggagaaaa
     2461 agagatcgag gagataaccg tgggcaggga tgaaaagtgg aagaagatca tgaagaactg
     2521 gagggtctga agaacctgga agaccgcaag tgatccttta ccccttcaga tgcctggtcc
     2581 tttcaccttt tctgcgggaa cagggatcca tttagcacaa gagggagcgt ccagcgccga
     2641 taaccagctt cccatgacca gacgctccct gcatggatcc ggcgtgactg attaaaatct
     2701 gaaacgacca gacagttatg atcacagtta tcattccata aaagaaacgc ccgaatagaa
     2761 aagtcgagag catccccttc cctgaccggt attgacattc aaagacaaaa tggtgaaaat
     2821 gaagaaaaat tttcaccagg agcctccttt gaataaaaat aaacatcact ctgttcttga
     2881 aaggatcacc cggttttcga actccctggg cggacagcag agggctctcg cccgtttcat
     2941 ccttcagaat cccgaaacag cggctttcat gaactccgta caactttcgg aagcggccaa
     3001 agtaagcaac ccgactgtga caaggcttgc tatcaaactg ggattcaagg gtttccctga
     3061 gttccaggag gccctccagg agactgtccg ggcacagatc cgttccataa gacgattcat
     3121 ggaggatccc ggggtagaag aggaacccct cttccagaga gtgctctctc ttgagttcca
     3181 tgtccttggt gagatgaaaa gacgcctgaa ggaggagacc ctccagaggg cggtagacct
     3241 gatatcagag aaacgcagga tcttcattgt tggtttcctt gccaatatct gccacgccga
     3301 gtacatggcc tacttcctgg ggaccctcag ggaaggggtt cacctgctga cgcgggccgg
     3361 tgacaacctc ttcgggcatc ttcagaacac cggtcccgag gatctggcaa taatatacag
     3421 ttttccccgt tatccctcgt cgacgcagaa gcttgcctta tacctcagat ccaaggacgt
     3481 accgataatc ggggtgagcg acacggttct ttctccattg gcttcatgtt cggacattct
     3541 tcttgaagct cccatgaaat tcctctcctt catcgacccc tgcgccggag ccttcgctct
     3601 caaccactgc cttcttacgg ccttctactt caggtacccg gaaaagatgc gtgaggctct
     3661 acgaatgttc gaggaatact cccacagaca gaacctgttc ctgatggaag atatcgacat
     3721 cgtcgatctc gtataaaacc caatgtcagg aggcgtgatc tgaatggaag ttatgctcat
     3781 ctcccagaaa gagatcgaat cgcttcgtat ccctgtgacc gaggtcatgg acgttgtaga
     3841 gaaggggttt gccctgaaag gggaagagaa gatggagatg ccggccaaag taggtatcca
     3901 tccccgaaag gactgcttta tccacgccat gccctgctat gcggggggca gcgcagacgt
     3961 tgcgggcatt aagtgggtct ccggatatcc tatcaatcag gccaagggac ttccctacat
     4021 caccgggatc atgtgcctca acgatcccga taccggtttt gtcaaggtct tgatggatgc
     4081 caactggata actgcctgga ggacaggggc cgcaaccggg gtttgcgcaa ggcacatggc
     4141 cgaccccgat tcagaggtgt tggccgtggt cgggcttggg gttc
//
LOCUS       DAOPKQ010000119         4177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_23394_length_4177_cov_3.911208, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000119 DAOPKQ010000000
VERSION     DAOPKQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23394_length_4177_cov_3.911208"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PK146_05015"
     CDS             <1..952
                     /locus_tag="PK146_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPE91636.1"
                     /translation="EEFSHIRDPHTGRPLMDRMVLIANTSNMPVAAREASIYLGMTIA
                     EYFRDMGLDVALMADSTSRWAEALREISGRLGEMPGEEGYPAYLGSRLAQYYERAGRV
                     MALGEPRRKGSITIFNAVSPGGGDFSEPVTQASLRLSGAFWALDKRLAESRHFPSINW
                     KQSYSLYEKALEAYFMENLGKEWRDGVSFLRELLDRETELLSLVQIVGRDALSEQDKW
                     DLEMGEIIRITFLQQNAFDPLDAFNSLERQMQFAGTLKDLHELVREEIKKGVLHQQIQ
                     GLGLLRRVLSLRGTSTEEFQERSSAWLDDLTRSLSGLEGS"
     gene            954..2342
                     /locus_tag="PK146_05020"
     CDS             954..2342
                     /locus_tag="PK146_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPE91637.1"
                     /translation="MVLYREGSRLVNGISGPLVFVSNARKAALGEVVTIETGEGRRTG
                     QVLQVDGNICAIQVFEGTIGLNPGDCTVWFEKDVVKVRLGPSLMGQTLDGRGVPRSGK
                     ALPCFEKEAPITGSPLNPAMRASPSEFVETGISSIDLLNTLVKGQKLPIFSGSGLPAN
                     EIAAQVVRQAHVPGNPERFLVVFAAIGITEREARFFRDSFEETGALFNGVFIVNLASD
                     SAVERLLAPRTALTIAEYFAFDRGYDVLVVMTDMLHYCEALREVSSAREEAPGRRGFP
                     GYLYSDLASLYERAGCIKGINGSITQLPIVSMPGDDMTHPVVDLSGYITEGQIVLDRG
                     LQARGVYPPINVLSSLSRLMNKGIGEGKTFSGHRQVADQLFASYARSREASRLRLIVG
                     DEGLTETELHYLRFGSVFERVFLNQGDNALSIEESLSAGLKALSVLPDEELFRIPADL
                     VEETGRVRIDEQ"
     gene            2332..3009
                     /locus_tag="PK146_05025"
     CDS             2332..3009
                     /locus_tag="PK146_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPE91638.1"
                     /translation="MNSDVPVSRSELIEISRKIENMRFGCSLLERKKEALLREIETDR
                     ATFRTMRREVESMLSFVSYAYALVQLFEGESVMTLLSWGIEPRKVRIVRHALMGCKYS
                     QFFPEDEGRGPLLGGMLMDPALASMHVDELLETLARIEPVLWKYINLKSKLDALELEF
                     EKTRRKVNNLEQEMLPGLEGQQRRIRLALAERERDEAFSVKKMLGKRARTSRSRSFGI
                     QGRRGSN"
     gene            3015..3467
                     /locus_tag="PK146_05030"
     CDS             3015..3467
                     /locus_tag="PK146_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPE91639.1"
                     /translation="MILSVGRPKNPLYKNGIEEYHRRIGSCVPFQWEAVRSSGGKSSV
                     ASLREEGAAILKRINERDLLALLEDSGNSFTSEGFSRWLFEKLRGTGGRVILCVGGPF
                     GVSGAVKARADVLVSLSPMTLTHDMSLLLLFEQVYRAIAIEKGTGYHH"
     gene            3472..3840
                     /locus_tag="PK146_05035"
     CDS             3472..3840
                     /locus_tag="PK146_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPE91640.1"
                     /translation="MRQRRELLQALQLWRGEIRTMNMEQMMKQAQKLQSEIGRIQEEL
                     TRERVEGVSGGGMVKVTATGQGEIVCISIEPEVIDPEEKEMLEDLLLAAISDASRKSK
                     ELAQSRLGRFGAGLGIPGMG"
     gene            3851..>4177
                     /locus_tag="PK146_05040"
     CDS             3851..>4177
                     /locus_tag="PK146_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HPE91641.1"
                     /translation="MSISEPVEKLMKLFSRFPGVGDKTARRFALFLLQQPGEFSDELA
                     FTISEVRENTFPCSICGNLTTEDPCPVCSDPFRDRGTLCVLENVEDMISIEQAGVFSG
                     LYFILGG"
BASE COUNT          960 a         1038 c         1267 g          912 t
ORIGIN      
        1 tgaagagttc tcccacatca gggatcccca cacaggaaga cctttgatgg atagaatggt
       61 attgatagcg aacacctcga acatgcctgt cgccgcaagg gaagccagca tatacctcgg
      121 gatgaccata gccgaatatt tccgtgacat gggacttgac gtcgcgctca tggccgactc
      181 tacctcccgc tgggcagagg cgttgaggga gatatcggga cgcctgggcg aaatgcccgg
      241 tgaagagggg taccccgcct acctcgggtc caggcttgcg caatactacg agagggcagg
      301 aagggtcatg gccctgggag agccccgaag aaagggatcg atcaccatat tcaacgccgt
      361 ttcccccggg ggcggggatt tctcggaacc cgtcacccag gcgagcctga gactttcggg
      421 agctttctgg gccctcgaca aaaggcttgc cgagagccgc cattttcctt caattaactg
      481 gaaacagagc tactctctct acgaaaaggc tcttgaggcc tatttcatgg agaacctcgg
      541 taaggagtgg agagatggag tctccttcct cagggagctg ctggaccgtg agacggagct
      601 tctctcactc gtccagatcg ttggcaggga tgccctttca gaacaggaca agtgggacct
      661 ggagatgggc gagatcataa ggataacctt tcttcagcag aacgcctttg atcctctgga
      721 tgccttcaac tccctggaaa gacagatgca gttcgctggt accctgaaag atcttcatga
      781 actggtcagg gaggagatca aaaagggggt cctgcatcag cagatacagg gcctcggcct
      841 gttgcggaga gtcctttcac tcaggggaac atcgacagag gagttccagg aacggtcttc
      901 cgcatggctt gacgacctga cccggtccct ttccggattg gagggctcct gatatggtcc
      961 tttacaggga aggttccaga cttgtcaacg gtatatcggg acccctggtc ttcgtttcca
     1021 acgcaaggaa agcggccctt ggggaggtcg tgaccataga gaccggtgag gggaggagga
     1081 ctgggcaggt actgcaggtc gacggcaata tctgtgcgat ccaggtcttc gaggggacca
     1141 tcggactgaa cccaggcgac tgcactgtat ggttcgagaa ggatgtggtc aaggtccgcc
     1201 tgggaccctc cctgatgggg cagaccctcg acggccgggg tgttccaagg agcggcaagg
     1261 cacttccctg ttttgagaag gaggctccta taacaggttc ccctcttaac ccggccatga
     1321 gagcgagccc aagtgaattc gttgagacgg gcatatcgtc catagacctt ctcaacaccc
     1381 tggtgaaagg ccagaaattg cctatctttt cggggtcagg cttacccgct aatgagatag
     1441 ccgcccaggt ggtcaggcag gctcatgttc cgggtaaccc cgagagattt ctggtcgtct
     1501 tcgccgccat aggaataacg gagagggaag ccaggttctt cagggattcc ttcgaggaga
     1561 cgggagctct cttcaacggg gtcttcattg tcaaccttgc aagcgactcc gccgtggaga
     1621 ggcttcttgc gccaagaacg gccctgacga tagcggagta ttttgctttc gatcgtggct
     1681 atgacgttct tgttgtaatg acggacatgc ttcactactg cgaagccctc agagaggtta
     1741 gttccgctag ggaggaggcc ccgggtagaa gaggcttccc gggatacctc tattctgacc
     1801 ttgcaagcct ctacgaaaga gccggatgca taaaggggat taatggtagt ataacccagc
     1861 tgcccattgt ttcaatgccg ggggatgaca tgacccaccc ggtggttgac ctttcagggt
     1921 acattactga aggccagata gtcctggaca gggggcttca ggcgagaggt gtctaccctc
     1981 cgatcaatgt tctttcgagt ctcagcaggc ttatgaacaa agggatcggc gaagggaaga
     2041 ccttttctgg tcataggcag gtagctgacc agctttttgc ctcctatgcg aggtccaggg
     2101 aagcatccag gctgaggctg atcgtagggg atgagggcct tacagaaacg gagcttcact
     2161 acctccggtt cggttcggtc ttcgagagag ttttcctcaa ccagggcgac aacgctcttt
     2221 ccatagagga atctttgtcg gcggggttaa aagccctctc ggtcctcccc gacgaggaac
     2281 ttttcaggat ccccgccgat cttgttgagg agacagggag agtccggatc gatgaacagt
     2341 gatgtccccg tatcaaggag cgaactgatc gagatctctc ggaagattga aaatatgcgt
     2401 ttcggctgtt cccttctgga aaggaaaaaa gaggccttgc taagggagat cgagaccgac
     2461 cgggctacct tccggacaat gaggcgggaa gttgagagca tgctatcttt cgtctcttac
     2521 gcctatgccc ttgtccagct cttcgagggt gaatcggtga tgacgctgct gtcatggggg
     2581 atcgaaccgc ggaaggtcag gatcgtccgt catgccctta tggggtgcaa gtactcccag
     2641 ttcttccctg aggacgaggg gaggggacct ctcctgggag gaatgcttat ggatcctgcc
     2701 ctggcttcga tgcacgtgga tgagcttttg gagaccctgg ccaggataga acctgttctc
     2761 tggaagtaca ttaacctcaa atccaaactg gatgccctgg agctggagtt cgagaagacc
     2821 aggagaaagg tgaacaacct tgaacaggag atgctcccgg gactggaggg gcagcagaga
     2881 aggataaggc tggccctggc tgagagggag agggatgaag ccttctcggt caagaagatg
     2941 ctcgggaaaa gggcaagaac ctcccgttcc cgatctttcg ggatccaggg cagacgtggc
     3001 tccaattgaa gcttgtgatc ctttcagtgg ggagacccaa aaaccccctt tataaaaacg
     3061 gaatagagga gtaccatcgg cggataggca gttgcgttcc gttccagtgg gaggcggtaa
     3121 ggagttccgg aggcaagtcc tccgtggcat ccctccggga agagggtgcg gcgatcctca
     3181 aaaggatcaa tgagagggac cttcttgccc ttctcgaaga ctccggcaac agcttcacca
     3241 gcgaagggtt ctcccgttgg ctctttgaaa aactccgagg caccggcggt cgtgtgatac
     3301 tgtgtgtggg aggccctttc ggtgtttcgg gtgcggtaaa ggccagggcc gatgtgctgg
     3361 tatccctttc cccgatgacc cttactcacg acatgagcct cctgctcctc ttcgaacagg
     3421 tctacagggc tattgcgata gagaaaggga caggttatca tcattgaccc gatgcgacag
     3481 agaagggaac ttttacaggc tctacagcta tggaggggtg agataaggac gatgaacatg
     3541 gaacagatga tgaaacaggc gcagaaactc cagtccgaga taggaaggat ccaggaggaa
     3601 ttgaccaggg agagggtaga aggagtatca gggggtggaa tggtcaaggt aacggctacg
     3661 ggtcagggag agatcgtatg tatctccatt gagcccgaag ttatcgatcc cgaagagaag
     3721 gaaatgctgg aggacctcct ccttgccgcc atcagtgatg cttcaaggaa gagcaaggaa
     3781 ctggcccagt cccggcttgg caggttcgga gctggtctgg gtattcccgg gatgggatag
     3841 gaggctggag ttgtccattt ctgaacccgt agaaaagttg atgaaactct tttcccgctt
     3901 tccgggagtg ggtgacaaga cggccagaag gttcgccctt ttccttctgc agcagcctgg
     3961 agagttctca gatgaacttg ccttcaccat atctgaagtt cgggagaaca ctttcccttg
     4021 ctccatatgc ggcaacctga ccacggagga tccttgccca gtatgctcgg acccctttcg
     4081 ggacaggggc acactctgtg ttctggagaa cgttgaagat atgatatcca ttgaacaggc
     4141 cggtgtcttt tcggggctgt attttatcct tggcggg
//
LOCUS       DAOPKQ010000120         4131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_23757_length_4131_cov_4.823847, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000120 DAOPKQ010000000
VERSION     DAOPKQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23757_length_4131_cov_4.823847"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="PK146_05045"
     CDS             <1..1746
                     /locus_tag="PK146_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPE91642.1"
                     /translation="PQDLVEIGNAPLMELLKEKTGLARVEKDLNEVRNRLSGLKPHSA
                     ERSSLLDRHDRLVFSYEALDGYGFEAMAAKILKGLGFRSGDRDRMSGEFSGGWKMRIS
                     LASLLLSGPDVLLLDEPTNHLDTESMEWLEGYLASFRGTLLAISHDRRFLDKLTGTTV
                     EISGGRARSYPGNFSEYVTKRSRELEEAEKLAKRQEKEVARTVSFIERFRYKASKAAQ
                     VQSRIRALDKIDRIETAKASRRVSMRFPPCPRSGLEVISAIEVEHSYGDNRVFSGVNL
                     SIRRGERVALVGVNGAGKSTLTRLLSGEERPTRGSISLGHNVKSAFFSQESSKNLNYG
                     NSVWDEIRNAPSPCTEPQRRNLLGCFLFSGSDIEKPVRVLSGGERSRLALLKILLRQT
                     NLLILDEPTNHLDMVTRDLFQEALLGYGGTIVIVSHDRDFLDNLVTRVIEVRDGGIFD
                     YPGNYSYFIEKRAEKPAQSGPQTAGRASADNPVRINLREEKRADAERRNRLYRNRKKF
                     LEELEPLETKIDRTEERIGEIDLSLSAPSTLSDSGKVKELLLERADLERTLLPLMKRW
                     EELMRSLEDAGQEIH"
     gene            complement(1760..2629)
                     /locus_tag="PK146_05050"
     CDS             complement(1760..2629)
                     /locus_tag="PK146_05050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPE91643.1"
                     /translation="MTVFRLLVAAATFLFLGSAAARPSLAVNVIAPVETSIGRPFLVF
                     VSSKRPLEEVVIFWEGKEIPLAVERSGQTSEAMTLLGTDVGSSKPGAKTLEVRSSVGG
                     KPVSVTRQVNVRHVDFPVQKLSLPQEMVDPPREVLERIARERKETARALSTFTRSRVW
                     AIPFVRPVPGKITSPYGFRRILNDQPRAPHRGVDYRAAEGDPVRAAADGTVVLSAEHY
                     YAGQCIYLDHGGGVVSVYMHLSSRFAEEGETLSAGDIIGLAGRSGRVTGPHLHFGLSL
                     QGMMVDPEPLLES"
     gene            complement(2653..3639)
                     /locus_tag="PK146_05055"
     CDS             complement(2653..3639)
                     /locus_tag="PK146_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPE91644.1"
                     /translation="MKMDPGADTVRVAVVQAAPVIMNREGTVQKALDLMDVCGRENAR
                     IVVFPEAFIPAYPRGLTFGTVVGSRSPGGRKDFRRYFENSVEVPSETTAALGEAARKN
                     GAYLVMGVIERDGGTLYCTILYFGPDGRLLGKHRKLKPTGSERILWGEGDGSTMTVID
                     TPYGKMGGLICWENYMPLARAAIYGKSTTIYLAPTADSREEWQATIRHIALEGRCFVL
                     SVNQYVHKSMYPEDLACFTDLKDQPDIMCPGGSAIIGPMGQYLAGPVRDREEILFADL
                     DPGAIAESRFDLDVTGHYARSDVLALVVNEKPLASVSFVDGGDACDQGLAEK"
     gene            complement(3636..>4131)
                     /locus_tag="PK146_05060"
     CDS             complement(3636..>4131)
                     /locus_tag="PK146_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE91645.1"
                     /translation="LTMIMRYWGEERFYHHTAPVNMIYGLNEGLRVIAEEGLENRFAR
                     HRYNAEALWAGLEAMGLELLVEPAYRLPSLTSVKMPQGVDEALVRRRLMDEYSIEVGS
                     GLGDLKGKIIRIGLMGSGSNRRNIVLFLSALGEILSGEGFSNDTGAALDEALSVIEGK
                     RTVE"
BASE COUNT          913 a         1267 c         1168 g          783 t
ORIGIN      
        1 ccccaggacc ttgtagagat aggcaacgct ccccttatgg agctcctcaa ggaaaagact
       61 ggattggccc gcgtcgagaa agatctcaat gaggtccgca acaggctttc gggactgaaa
      121 ccccattccg cggagaggag ctctctcctt gaccgtcatg acaggctggt cttctcctat
      181 gaagccctgg acgggtacgg attcgaggcg atggctgcga agatacttaa aggactgggg
      241 ttcaggagcg gcgacaggga tcgaatgtcg ggagagttct ccggtggatg gaagatgcgc
      301 atatccctgg cgtcactttt gctttcagga cctgacgtcc tccttctcga cgaaccaacc
      361 aaccatctcg atacggagag catggagtgg ctggagggat acctcgcatc cttccggggc
      421 accctgctgg ccatatccca cgacagacgc ttccttgaca aactgaccgg gaccaccgtg
      481 gagatatccg gcgggagggc acgttcctac cctggcaact tctcggagta cgtcacgaag
      541 aggtcccggg agctggaaga agcagaaaag ctggcaaaga ggcaggagaa ggaggttgca
      601 aggacggtca gttttatcga gcgcttccga tacaaggcat caaaggcggc ccaggtacag
      661 agccgcatca gggcgctgga caagatcgac aggatcgaga ccgccaaagc ctcgaggaga
      721 gtctcaatga gattccctcc atgtcccagg agtggtcttg aggttatcag cgccatcgaa
      781 gttgaacact cctacggaga taacagggtc ttctccgggg tcaacctctc catcaggcgg
      841 ggcgagcggg tggccctggt cggggtgaac ggcgcaggga aatcgaccct tacaagactc
      901 ctcagcgggg aggagagacc gacccgggga agtatctccc tggggcacaa cgtaaaaagc
      961 gccttcttct cccaggagag ctcgaaaaat ctcaattacg gcaactcagt atgggacgag
     1021 ataaggaacg ccccttcccc ctgcacggaa ccccagcgca ggaacctcct aggatgcttc
     1081 ctcttttcgg ggagcgacat cgaaaaaccg gtccgggtac tctccggcgg ggaaagatcc
     1141 aggctggccc tgctcaagat ccttctcagg cagaccaacc tgctcatcct ggacgaaccc
     1201 accaaccacc ttgacatggt caccagggac ctcttccagg aagccctcct cggctacggc
     1261 ggaactatcg ttatcgtctc ccacgacagg gacttcctgg acaacctggt caccagggtc
     1321 attgaggtca gggatggggg tatattcgac tacccgggga actactccta cttcatcgaa
     1381 aagagggcgg agaaaccggc ccaaagcgga ccgcagacag caggaagagc ctccgctgac
     1441 aacccggtca ggataaacct cagggaggag aagagggccg atgcagaacg caggaaccgg
     1501 ctttacagga acaggaagaa gttcctcgaa gagctggaac ctctcgaaac aaagatcgac
     1561 aggaccgagg agaggatcgg tgagatagac ctttctctct ccgccccctc tacactgtca
     1621 gactccggaa aagtcaaaga acttcttctg gagagagctg accttgaaag gacgctcttg
     1681 cccctgatga aaagatggga ggaactgatg agatcgctgg aggatgcggg tcaggagatc
     1741 cactgactcc ggatccctgc taactctcga gaaggggctc gggatccacc atcattccct
     1801 gaaggctaag gccaaagtgc agatggggtc cggtcaccct cccactcctg ccggcaagcc
     1861 cgataatatc cccggccgac agggtctctc cctcctctgc aaatctgctg gaaaggtgca
     1921 tgtagaccga taccacacct cccccgtggt caagatatat gcattgaccg gcgtagtagt
     1981 gttcagccga aaggaccacc gtaccgtcag cggcggccct gacgggatcg ccttcggcag
     2041 cccggtagtc cactccccgg tggggagcgc ggggctggtc gttgaggatc ctccggaaac
     2101 cgtatggact ggtaatttta cccgggacgg gacggacaaa agggatagcc cacaccctcg
     2161 acctggtgaa cgtggagagg gctctggccg tctccttgcg ctccctggcg atccgctcca
     2221 gtacctccct gggagggtcc accatctcct gggggaggga tagtttctgc acggggaagt
     2281 ccacgtggcg gacgttgacc tgcctggtga ccgacaccgg tttgcctccg acggaggacc
     2341 ggacctccag ggtctttgcc cccggcttcg agctccccac atcggtcccg aggagggtca
     2401 tggcctcact ggtctgaccg gatcgctcca ccgcaagggg tatctccttc ccctcccaga
     2461 agatcaccac ctcctccagc ggcctcttgg acgaaacaaa aacaaggaat ggccttccga
     2521 tggaggtttc cacgggggct atcacgttga ccgccagaga aggacgggct gccgcagacc
     2581 caaggaacag gaacgtcgcg gcagcgacca gtaacctgaa caccgtcatc gagggcgatc
     2641 gcggccaggc catcatttct ccgccagccc ctgatcgcag gcatcgcctc cgtcaacgaa
     2701 ggatacgctt gcgaggggct tctcattaac cacaagagcg aggacatcgg atcgagcgta
     2761 gtgaccggtc acatcaagat cgaagcggct ttccgctatg gccccagggt cgaggtccgc
     2821 aaagaggatc tcctccctgt ccctgacagg ccccgcaagg tattgaccca taggaccgat
     2881 gatcgcgctt ccgccggggc acatgatatc cggctggtcc ttcaggtcgg tgaaacaggc
     2941 aaggtcttcg ggatacatag acttgtgaac atactggttc acggagagga cgaaacatct
     3001 gccctcaagg gcaatgtgac ggatcgtagc ctgccactcc tcgcgggaat ccgccgtcgg
     3061 tgcaaggtag atagttgtac tcttgccgta tatcgccgcc ctggcgagag gcatgtagtt
     3121 ctcccagcag ataagtccgc ccatcttgcc gtagggggta tcgatcacgg tcatggtgct
     3181 gccgtccccc tcaccccaca ggatcctctc cgatcccgtt ggttttagtt tgcggtgttt
     3241 cccaagaagt ctcccgtcgg ggccgaagta gaggatggtg cagtacaggg tcccgccatc
     3301 ccgttcaatg acgcccatca ccaggtaggc cccgttcttc ctggcagcct cccccaaagc
     3361 ggcggtggtc tctgatggca cctccacgct gttctcgaag tacctccgga aatctttcct
     3421 gcccccggga gatcggctac ccaccactgt cccgaaggtc aaaccccggg ggtaggcagg
     3481 gataaaggcc tcggggaaga ccacgatcct ggcgttctcc cggccacaga catccatcag
     3541 atccaatgcc ttctggaccg tgccctccct gttcatgatc acgggggcag cctggactac
     3601 ggctaccctt actgtatctg cccccgggtc catcttcatt ctactgttct cttcccctcg
     3661 attaccgaca aggcctcatc cagtgctgca ccggtatcat tggagaaccc ttcaccggac
     3721 aggatctcgc ccagcgccga aaggaagagg acaatgttcc tccggttgga gccactaccc
     3781 ataagcccga tccttatgat cttgcccttg aggtccccaa gcccggaccc gacctctatg
     3841 gagtactcat ccatgagcct tcttctgacg agggcctcgt ccaccccctg aggcatcttc
     3901 acggaggtaa ggctcggaag cctgtaggcc ggttcgacca gaagttccag gcccattgcc
     3961 tccagtcctg cccatagcgc ttctgcgtta tacctgtgac gggcaaacct gttctcgagc
     4021 ccctcctcgg cgataaccct caaaccctcg ttcagcccgt aaatcatgtt cacaggggct
     4081 gtatggtggt agaacctctc ctcgccccag tagcgcatga tcatggtcag g
//
LOCUS       DAOPKQ010000121         4051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_24400_length_4051_cov_5.456206, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000121 DAOPKQ010000000
VERSION     DAOPKQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24400_length_4051_cov_5.456206"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PK146_05065"
     CDS             complement(<1..1206)
                     /locus_tag="PK146_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPE91646.1"
                     /translation="MAINAAGRNIPDSIPGYGEVRHFKGHDRRPPEGNRFGKRNRTAF
                     NTRTDKTLPDVKAAISASGLKSGMTISFHHHLRNGDFVVNAVLDACAEMGIKDLVIFP
                     TALFGVHKHLTKHIKSGVIRQIMGSVNGPIGQLASEGGMSVPVVLRSHGGRPRAVTSG
                     EVVIDVAFIAAPTADKYGNLSGAVGRSACGSLGYAHSDAEYAQCVVAITDNLIPYPAP
                     CIHIPQFFVDFVVSVDSIGDPAGIVSGTTRVTRDPMRLLIARNAAKLIEATPYFRDGI
                     SFQTGAGGISLAVAAYVRDAMREKGIKGSFGLGGITEFFVDMMKEGLFERLLDVQSFD
                     LAAVKSIGENPNHVEISADWYANPWNAGCAVNMLDTVVLGATEVDRDFNVNVNTEADG
                     ALLHGIGGHQ"
     gene            complement(1206..2084)
                     /locus_tag="PK146_05070"
     CDS             complement(1206..2084)
                     /locus_tag="PK146_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPE91647.1"
                     /translation="MRLRRTKLYLPGNNPNMLLRGHLFNPDGVILDLEDAVAVSEKDA
                     ARILVRDVLRRGDFGSCEVTVRINAMDTEFWRTDIETVVPCGIDGIRIPKVENPKDIA
                     LMDEALSQIESKAKIRVGKTLIFCLLETALGIWRAYDIAKASRRVAAICPGGEDLRAD
                     LKTTRSPNSEELIGPRRMVVLAAHAAGVDALDTVFADITDDAGLRRESEWVKQLGYQG
                     KSVIHPNQIPIIHDVFTPTAKEIEQAKKIVEAAAEAAARGLGAVQVDGKMVDKPVVKR
                     AEFTLQRAGLKAGGEK"
     gene            complement(2081..2353)
                     /gene="citD"
                     /locus_tag="PK146_05075"
     CDS             complement(2081..2353)
                     /gene="citD"
                     /locus_tag="PK146_05075"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HPE91648.1"
                     /translation="MKTAQAGTLESMDCIVTVTEAEKGRGISIVMEGASVARFGEAMV
                     KATKDTLKSLGVRDIFVSIQDNGAGDLVLKARVEAAVRRLAGGKAA"
     gene            complement(2764..3999)
                     /locus_tag="PK146_05080"
     CDS             complement(2764..3999)
                     /locus_tag="PK146_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPE91649.1"
                     /translation="MARNVMQVKEKKDRYQVAYIPGDDSGFDVMEAAMIVMQALDAPI
                     DWVRADMGWCMWEKTCKEFPEGDKRRNTVPQETIDKVRNTDATLMAAITSKAGVKGFK
                     SAILQMRQIFDLFINFRPAKLMPGLECPLKGDPKIDIKLFRENTEDLYSAVEWRPLPR
                     EMFDLHPGMERFKDKSEVAVSWRVYSKEGCDRIIRAAFEYAKANGLKSVVCCNKANVI
                     RATDGMMKERFLEIAKEYPDIDSREENADATAMWLIKNPQDYQVIVTSNVFGDILSDE
                     ASQLVGGMGFAPSGNIGEEAALFEPSHGSVPKYAHQYKINPCGLLITAVMMLEYLGLN
                     DHAARLEKAIGDVLVESKYLTYDIWRDDKGDKDWEKKAVSTLEMAKAIAGKIDPDFDK
                     KADEICAKAREMCAWEHLA"
BASE COUNT          676 a         1213 c         1156 g         1006 t
ORIGIN      
        1 ctggtggccg cctatgccgt ggagaagagc tccgtcggcc tcggtgttca cgttcacgtt
       61 gaagtccctg tccacctcgg tggctcccag cactaccgtg tcaagcatgt tcacggcgca
      121 ccccgcgttc caggggttgg cgtaccagtc ggccgatatc tccacgtggt tggggttctc
      181 gcctatggat ttaaccgcgg caaggtcgaa ggattgaacg tcgagaagcc tttcgaagag
      241 cccctccttc atcatgtcca cgaagaactc cgtaattcct ccgagcccga agctcccctt
      301 gatccccttc tcccgcatgg catcccttac gtaggccgcc actgcaaggg atatgccacc
      361 tgctccggtc tggaaggata tgccatccct gaagtagggt gtcgcttcga tcagcttcgc
      421 ggcgttcctg gctatcagaa gcctcattgg gtccctggtc acccgggtag tgccgctgac
      481 gatccctgcg gggtcaccga tgctgtctac agagacaacg aagtccacaa agaactgggg
      541 gatatggatg cagggcgcgg ggtagggaat caggttgtct gttatggcca caacgcactg
      601 tgcgtactcg gcgtcgctat gggcgtaccc gagggacccg caggctgatc tgcctaccgc
      661 tcccgaaagg ttgccgtact tgtccgccgt gggcgctgca atgaaggcca cgtcgataac
      721 gacctcgccg cttgtcacag ccctgggacg ccccccgtgg ctcctcagga cgacggggac
      781 gctcatgccg ccctcggagg caagctgtcc tatggggccg ttgacggaac ccatgatctg
      841 gcggatgacc ccgctcttga tgtgtttcgt cagatgtttg tgaacgccga aaagagccgt
      901 tgggaagata acaaggtctt tgatccccat ctcggcgcag gcgtcgagca cggcgttgac
      961 cacgaaatcg ccgttgcgga ggtggtggtg gaaggagatg gtcatgccac tcttcaaccc
     1021 cgacgcgctg atggcagcct tgacgtcggg aagggtcttg tcggtccttg tgttgaaggc
     1081 cgttctgttc ctcttcccga aacggttgcc ctcgggcggt cttctgtcat ggcccttgaa
     1141 gtgcctgacc tccccgtaac ccggtatcga gtcgggaata ttacgtcctg cggcgttgat
     1201 agccattact tttcacctcc ggctttgaga ccggcccgct ggagagtgaa ttccgccctc
     1261 ttgaccacgg gcttgtcgac catcttcccg tcgacctgga ccgccccaag acccctggcc
     1321 gcggcttcgg cggcggcctc cacgatcttt ttagcctgtt ctatctcctt ggctgtcggc
     1381 gtgaagacat cgtggattat cggtatctgg ttcggatgga tgacgctctt gccctggtag
     1441 cccagctgtt tgacccattc actctcccgt ctgagaccgg cgtcgtcggt gatgtcggcg
     1501 aagacggtgt ccagggcgtc gacacctgcg gcgtgggcag ccagaacgac catccttcgg
     1561 ggtccgatga gctcttcgct gttgggagag cgggtcgtct tgaggtcggc cctgaggtct
     1621 tcacctccgg ggcagatggc ggccaccctt ctggaggcct tcgctatgtc ataggccctc
     1681 cagatgccaa gggccgtctc tagaaggcag aagatgagag tcttgcctac acggatcttg
     1741 gccttgctct ctatttggga gagagcctcg tccataaggg cgatatcctt cgggttttcc
     1801 accttgggta tgcggatgcc gtcgataccg cagggcacga cagtctcgat gtcggttctc
     1861 cagaattcgg tgtccatggc gtttatcctc acggtgacct cgcagctgcc gaaatcgccc
     1921 cttctgagaa cgtccctgac cagtatgcgc gcagcgtcct tttcgctgac cgctacggca
     1981 tcctcaaggt caaggatgac accatcgggg ttaaaaaggt gccccctaag gagcatattg
     2041 ggattgttgc cgggcaggta cagctttgtc cttctcagtc tcatgcggcc ttcccccctg
     2101 cgagccgtct cacggccgcc tcgaccctgg ccttgagcac aaggtccccg gctccattgt
     2161 cctggatgga gacgaaaatg tcccggacac ccaggctttt gagggtgtcc tttgttgcct
     2221 ttaccatagc ctcgccgaag cgggctacgc ttgccccctc catcacgatg gatatgcccc
     2281 gacccttttc tgcttcggtc acggtgacga tacagtccat cgattcgagg gttcctgcct
     2341 gggctgtttt catcttctta cctccctgtg tcaagcgacg tacttcgtct tgccgttcat
     2401 cgctttcaaa aaaccggggc ccacccgaag gtgagccccg ggctcattga tctttcctgg
     2461 ttatgccctt gcggtttacg atccgcgatt aagggttttg caggttttca ggttttgcat
     2521 tcccgttcac cgttgaccgt tcactgttaa cgcctttagg ttttgcccct tccgtctcac
     2581 gcctaacgtc tcaagctctt aaggtcttcc tgccgcctgc gttgcatagg gttttgaagt
     2641 gggtttcccc ataaacaaag gtttttgctc ttctctgtgg ccccaccctc tgcgttctct
     2701 gtggtgagat gccttggcct tgggtttacc ttctcacgtc tcacgtctca cgtctcacgg
     2761 tttttatgcc agatgctccc aggcgcacat ctcgcgtgcc ttggcgcaga tctcgtcggc
     2821 cttcttgtca aaatcggggt cgatcttgcc ggcgatagcc ttggccattt cgagggtgga
     2881 aactgccttt ttctcccagt ccttgtcgcc cttgtcatcg cgccagatgt cgtaggtgag
     2941 gtacttgctc tccacgagca cgtcgccgat ggccttctcg agcctggcgg catggtcatt
     3001 gagccccagg tattcgagca tcatgaccgc cgtgatgagc aggccgcagg ggttgatctt
     3061 gtactggtgt gcgtacttcg gaaccgaacc gtggctcggt tcgaagagag cggcctcttc
     3121 gccgatgttg ccgctgggtg cgaatcccat gccgccgacc agctgtgaag cctcgtcgga
     3181 gaggatgtcg ccgaagacgt tggaggtgac gatcacctgg tagtcctggg ggttcttgat
     3241 aagccacatt gcggttgcgt cggcgttctc ttcacgggaa tcgatatccg ggtactcctt
     3301 ggcgatctcc aggaagcgct ctttcatcat gccgtcggtg gccctgatga cgttggcctt
     3361 gttgcagcat accacgctct tgaggccgtt ggccttggcg tactcaaagg ctgcacggat
     3421 gatcctgtcg catccttcct tggagtagac cctccaggat accgcaactt ccgacttgtc
     3481 cttgaagcgc tccatgcccg gatgcaggtc gaacatctcg cgggggagcg ggcgccactc
     3541 cacggccgag taaaggtcct cggtgttctc gcggaagagc ttgatatcga tcttggggtc
     3601 gcccttcagg gggcattcga ggccgggcat caactttgcg gggcggaagt tgatgaagag
     3661 gtcgaagatc tggcgcatct ggaggatggc cgacttgaag cctttcactc cagccttgga
     3721 ggtgatggcg gccatgaggg tggcgtcggt gttcctgacc ttgtcgatgg tctcctgggg
     3781 tacggtgttc cttctcttgt caccctcggg gaactccttg caggtctttt cccacatgca
     3841 ccagcccatg tcggcgcgaa cccagtcgat gggcgcgtca agggcctgca tgactatcat
     3901 tgcggcttcc ataacgtcga agcccgaatc atcccccgga atataggcta cctggtatct
     3961 gtccttcttc tccttgactt gcataacgtt cctggccata ttattgcctc cttgttttaa
     4021 aaagcgtaac ttgtaactcg ttacttgtta c
//
LOCUS       DAOPKQ010000122         4025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_24610_length_4025_cov_4.236524, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000122 DAOPKQ010000000
VERSION     DAOPKQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24610_length_4025_cov_4.236524"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PK146_05085"
     CDS             complement(<1..162)
                     /locus_tag="PK146_05085"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HPE91650.1"
                     /translation="MYLGKADVNTYEKGSSREFLTTNGWGGFGFSTVIGANTRREHGL
                     LVVKKRGEEA"
     gene            complement(190..1083)
                     /gene="whiA"
                     /locus_tag="PK146_05090"
     CDS             complement(190..1083)
                     /gene="whiA"
                     /locus_tag="PK146_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPE91651.1"
                     /translation="MEKLREVIWDDWSSVLVDDRVKASVEIGGIISGLSGGERRMEAV
                     FSSSRFFVFRKLKRLWEVSDWGRPGETAPNLVIPSGMRGKVSLWLDQSMVHEKMSLYG
                     DPKRNRANTWHWLRGIFGSCGSLFLPRTGYYMVFRTSKAWGRDPSERLGQLLAMNDIS
                     FRRRTRYGFSETVIRDQSSIVGMLSNMQMFRTSLLLEEKAMLRSMKERANKVVNCDAS
                     NIRKSLETAKRQLETAEELIVTGKIRMLSEAFRELVYARMENPSATLRELGQVLSKPV
                     SKSTVEYRWRKIEHLAGISSE"
     gene            complement(1071..2210)
                     /gene="yvcK"
                     /locus_tag="PK146_05095"
     CDS             complement(1071..2210)
                     /gene="yvcK"
                     /locus_tag="PK146_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HPE91652.1"
                     /translation="MNWWSAFTIGLLTGGIISLLAARLFQEEEDGGFREPQTGKNGSI
                     MAKAFQHKVSQGPEIVAIGGGTGLSTLLSGLKSYTRNITAIVTVTDEGGSSGRLRGEW
                     GVLPPGDIRNCMVALAENDSSLNRILNFRFDRGELKGHSLGNLILLATTEMTGDFRKA
                     VEELNNLLAIRGKVIPITNESVTLCAATGDGDFLRGELDISMRGHQIESVWIEPPDAK
                     AVPDAILAIENADVVVLGPGSLFTSIIPNLMFSEVSEALKRTAAPKIYITNLMTQPGE
                     TDGMSIMDHVRWITRTSGSVPDYVFTNEGEVPSDLLETYLAAGAVPLMVEEEQKRSLS
                     LMGSRVVVSDFLDVSGGSVRHNSQKIAEALIRIAREERDTSRWKS"
     gene            complement(2207..3091)
                     /gene="rapZ"
                     /locus_tag="PK146_05100"
     CDS             complement(2207..3091)
                     /gene="rapZ"
                     /locus_tag="PK146_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPE91653.1"
                     /translation="MKGSLSNRVSRCVIITGMSGSGKSTALHMLEDLGFYAIENIPPS
                     LLPQLIQVLGKHEEAVQNGVAAVVDVRGESLLDDLLAIIGSLRNRGLNIKVIFLDASD
                     SCLVKRFEETRRRHPLSSDLTILEAINRERRELSQIHEIADVVVETSDMALSQLRNEI
                     LGKMDISEGPLSVIFTSFGFKYGIPQDSDFVFDVRFLPNPFYVPSLRSMSGKDKEVQD
                     FILSQEGVSSFFGALSVLMEQVLSLYRNTGKNIVHIAVGCTGGRHRSVAVTEWLGSLF
                     PGNSSTRHRDIEKDTVIS"
     gene            complement(3116..3928)
                     /locus_tag="PK146_05105"
     CDS             complement(3116..3928)
                     /locus_tag="PK146_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPE91654.1"
                     /translation="MKRRVLGFSGRFGDRPLVYIHSDEKGGALVDGSPLGIVLAGAGE
                     GELGEGLFGIIVSEVPEIDPGDFLQFSDGVILKKSPENGTKGSFHVASKGFLSVSSLF
                     CPWTALKVGILTVSDKGSRGEREDTSGPALEKSVHSIGGFASMTGIVPDETESISGRI
                     REWIASGMDIILVTGGTGISRRDVTPEAVRSLGGKEIPGIGEYMRWKTSFDNERSILS
                     RSVAVACSGTIVVTLPGSERGAVQCFYSIAPVLRHAVEIVTGRSGDCAGSHR"
BASE COUNT          874 a         1205 c          975 g          971 t
ORIGIN      
        1 cgcctcttct cctctctttt taacaaccag cagaccgtgt tctcgtctgg tgttggcacc
       61 aataacggtc gagaagccga atccgcccca gccgttcgtc gtaaggaact cccgactgga
      121 gcctttctca taggtgttaa catcagcctt tcccaggtac atggccatca gtcccccttt
      181 ttgatctgtt tattctgaag agatccctgc aagatgctcg atctttctcc atcgatactc
      241 tacagtgctt ttgctgacag gcttcgaaag gacctgcccc agttccctca gggtcgccga
      301 ggggttctcc attcttgcgt aaacaagctc acgaaaggcc tccgatagca tacggatctt
      361 gccggtaacg ataagctctt cggcggtctc gagctgcctc ttggcggtct cgagggattt
      421 cctgatgttc gaagcatcac agttcacgac cttgttggcc ctctccttca tagacctaag
      481 catagccttt tcctcaagca aaagggaagt cctgaacatc tgcatgttgg aaagcatccc
      541 gacaatggag gactggtccc ttatgacagt ctctgagaaa ccgtaccttg ttcttcttct
      601 gaaggaaatg tcgttcatgg caagaagctg tcccagtctc tccgaagggt ccctccccca
      661 tgccttgctg gtcctgaata ccatgtaata acccgtcctt ggcaggaaca gggaaccgca
      721 ggagccgaag atccctctca gccagtgcca tgtattagcc ctgttcctct tgggatcccc
      781 gtaaagggac atcttctcgt ggaccattga ctggtccagc cacaacgata ccttgcctct
      841 catccccgat ggtatcacca ggttaggagc tgtttctcct ggtcttcccc agtccgagac
      901 ctcccatagt ctcttcagtt tccggaagac aaagaacctg ctgctggaga agaccgcttc
      961 catccggcgc tcccctcccg acaggccgga gatgatccct ccgatctcta cggaggcctt
     1021 taccctgtcg tcaacaagaa cgctcgacca gtcgtcccag ataacttccc tcagcttttc
     1081 catcgggatg tatctctttc ctccctggct atccggatca gggcctctgc gatcttctgg
     1141 ctgttgtgcc ttacacttcc gcccgatacg tcgagaaagt cggaaacgac aaccctggac
     1201 cccatgaggg agagggacct tttttgctct tcttccacca tcagagggac cgcgcccgct
     1261 gcaagatagg tctcaaggag atcggaaggt acttccccct cgttcgtgaa gacatagtcc
     1321 gggaccgatc cggatgtcct tgtgatccac ctgacgtgat ccatgatgga cattccgtcc
     1381 gtctccccag gctgggtcat gagattggtt atgtagatct tgggcgctgc cgttctcttg
     1441 agagcttccg agacctcgct gaacatgagg tttggaatta tgctggtgaa taggctcccg
     1501 ggacccagca ctaccacatc agcgttctcg atggccagaa tggcatcggg tacagccttt
     1561 gcatcggggg gctcgatcca gaccgactcg atctggtgcc ctctcatgga aatgtccagc
     1621 tcccccctga ggaagtcgcc atctcctgta gctgcacaca gggtgaccga ttcattggtt
     1681 ataggtatta ccttacccct gatagcaaga aggttgttaa gttcctcgac ggccttcctg
     1741 aagtcgccgg tcatctccgt ggtcgcaaga agaattagat tccccagact gtggcccttg
     1801 agctctcctc gatcgaacct gaaattcagt atccggttca gagagctgtc gttctcggca
     1861 agggcgacca tgcagttcct tatgtcaccc ggaggaagaa ctccccattc tccacgaagc
     1921 cggccggagc tgccgccttc atcggtaact gtaactatgg cagttatatt cctcgtatac
     1981 gattttaacc ctgaaagaag ggtcgaaagg cccgtacctc ctccgatcgc tacgatctct
     2041 ggaccctggg agaccttgtg ctggaaggct tttgccataa tagagccatt cttcccggtc
     2101 tggggctccc ggaaccctcc atcctcctct tcctggaaga gtctggcggc gagaagggat
     2161 attatgcctc ctgtcaaaag gccgatggta aacgccgacc accagttcat gatatcaccg
     2221 tatccttttc gatatccctg tgccttgtgg atgaatttcc cggaaatagt gaacccagcc
     2281 attctgttac agcaacggac cgatgcctgc ctcctgtaca gccgacagca atatggacaa
     2341 tgttctttcc ggtgttcctg taaagggaga ggacctgctc cataagcacc gaaagggcac
     2401 cgaagaaact ggaaacaccc tcctgggaga ggataaaatc ctggacctcc ttgtcctttc
     2461 cgctcatgga cctcagggaa ggaacgtaga aggggttggg aaggaaccgc acgtcgaaca
     2521 cgaaatcgct gtcctgggga atgccgtatt tgaaaccaaa tgaagtgaat atgacagaca
     2581 gagggccttc ggatatgtcc atcttgccaa ggatctcgtt cctgagctgc gacagggcca
     2641 tatccgacgt ctcaactacc acgtcggcga tctcgtgaat ctgggagagt tcccttcgct
     2701 ccctgtttat ggcctcaagg atagtgagat cagatgagag ggggtgtctc cttcttgtct
     2761 cctcgaaacg ttttacaagg caggagtctg aagcgtcaag aaagatgacc ttgatgttga
     2821 gacccctgtt cctgagactg ccaataatgg caagaagatc gtccaggagg ctctctcccc
     2881 ggacgtccac aacggcagcc accccgttct ggactgcttc ttcatgtttg ccgaggacct
     2941 gtatgagctg agggagcagt gacggaggga tgttctcgat ggcgtagaaa ccaaggtctt
     3001 caagcatgtg cagcgcagtg gatttcccac tccccgacat gcccgttatt atcacgcatc
     3061 ttgagaccct gtttgaaagg gaccctttca ataggggatc ccccttttcg ttattttatc
     3121 ggtgggatcc ggcgcaatcc ccactccttc ccgtcactat ctctacagca tgacggagaa
     3181 caggagcaat ggagtagaaa cactggaccg ctcccctctc gcttcccggc agcgtgacta
     3241 cgatcgtccc tgaacaggcc actgcgacgg aacgtgagag aatggacctc tcgttatcga
     3301 aggatgtctt ccatctcatg tattcaccta tccccgggat ctcctttcct ccaagggatc
     3361 ttaccgcctc cggagttacg tctctccggg atatgcccgt tcccccggtg accaggatta
     3421 tgtccatccc cgaagcgatc cattccctga tccttccgct tatgctttcg gtctcgtcgg
     3481 ggacgatccc tgtcatggag gcaaagcctc ctatggaatg aacagatttc tcaagggcgg
     3541 gcccggaagt atcctccctt tcaccacgac tgcccttgtc gctaacggtc agtatcccca
     3601 ccttcagcgc tgtccaggga cagaaaaggg acgagactga caaaaagccc ttgcttgcca
     3661 catgaaagga tcccttcgtc ccgttttcag ggctcttttt caggataacg ccgtcggaga
     3721 actggagaaa atcaccgggg tcgatctcgg gcacttcaga aacaatgatc ccgaacagac
     3781 cttccccgag ttcaccctct ccggctccgg caaggactat tccgagaggt gacccgtcaa
     3841 caagggcacc ccccttttca tcgctgtgaa tatacaccaa cggtcggtcc ccgaaccgac
     3901 ccgaaaaacc caaaaccctt cttttcacac cctgtcctcc gccctgtact ccccggatct
     3961 gccgccgctc tttctgagca gacggatcct ctcgatggtg atgcccttgt caataccctt
     4021 gcaca
//
LOCUS       DAOPKQ010000123         4020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_24650_length_4020_cov_4.088272, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000123 DAOPKQ010000000
VERSION     DAOPKQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24650_length_4020_cov_4.088272"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..431)
                     /locus_tag="PK146_05110"
     CDS             complement(111..431)
                     /locus_tag="PK146_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPE91655.1"
                     /translation="MDLIEKHPILEFKHGKKVKFTFDGNEMEGYEGEPIAAALHANGV
                     RIYRVTPVKEQTRGFFCAIGKCSSCFMVVDGVPNVRTCVTPLKTGMRVETQRGKGVIA
                     MDAE"
     gene            complement(596..1261)
                     /gene="tsaB"
                     /locus_tag="PK146_05115"
     CDS             complement(596..1261)
                     /gene="tsaB"
                     /locus_tag="PK146_05115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPE91656.1"
                     /translation="MRVLGIDCSTGWTGVGLFSEGAVKADVNYLAGRRQASLLPLLTS
                     EVLERSGLEPWSLDAVSVTVGPGSFTGIRVGLSYACALSEGIGRKVVPVSSLMALGAP
                     LLDGSEKILPLVRARRGQVYLAALGGERRSPFFLSPPRILSLEDLASSVELKSGFLTV
                     ADDDQALSRELSEMNIRVSNFASVRGGTVALLGGALFGQAVDPGEVRALYIRTPDTGA
                     PSG"
     gene            complement(1258..1809)
                     /gene="tsaE"
                     /locus_tag="PK146_05120"
     CDS             complement(1258..1809)
                     /gene="tsaE"
                     /locus_tag="PK146_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPE91657.1"
                     /translation="MIPFCHGQKSSKVKAGFFYLSRGPEETRHLASALSRYSFPGLVI
                     LLSGTLGAGKTEFVRGFASGSGWGDVRSPSFTIINEYLASTPITHVDLYRVERNASTE
                     FSLEEFSREGSIVLVEWPENWDGERPSEVWKIVFSVPEDLNSGRDAQNLRFLEVQCEG
                     GRACEMLERFMFGAGCELEEADR"
     gene            complement(1806..3353)
                     /locus_tag="PK146_05125"
     CDS             complement(1806..3353)
                     /locus_tag="PK146_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPE91658.1"
                     /translation="MYLFSADDIRLADRKAVDHFGIPPIILMENAARGVAEIVEDRFP
                     AASRILVACGGGNNGGDGLSAARHLLKRGRKVTVLLAAAEEKLTPEGSLNLKILKRIG
                     CPIARSEELTDVGMRELAMGQDILVDALLGSGSKGAPRGETGRVICAVRGTVPVVSID
                     IPSGVDPSTGEVEGDALEADLTVAILARKIGHEIMPGRSFRGEVETVDLGIEATLLLP
                     RSGKVVLVDPFIVSGFLPPLSPFIHKGDRGIVLVIGGSERYRGAPLLAALGALMAGSG
                     GVIAAVPAGSLPMFSRYPEIIPMEACTEDGTISPETWDRIVEMWKGRFKAVVIGPGLD
                     RGRVPQDLVRRIWKDWDGPLCLDGDALHAISCCSDLDPRAAGTVVTPHEGEAALLLSV
                     DSSEVKQCRYRSVLELSRRFGVALLKGPCSLVSESEKVRVNPHVLAGLSVPGSGDVLS
                     GVLGAFLARGINIFDAASSAAFVHGYAGGSVQERKGPDGITATEIALEVPSVLSRISA
                     AAGKVLP"
     gene            complement(3360..3725)
                     /gene="acpS"
                     /locus_tag="PK146_05130"
     CDS             complement(3360..3725)
                     /gene="acpS"
                     /locus_tag="PK146_05130"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPE91659.1"
                     /translation="MIKGVGVDMCSIPRLERALLDPRFVDKVFTESEREYASSRGGRT
                     RHLASAFAAKEAFSKAGGWGLGRVGLKNVSLERKEGAPFLILYGPALELIRSLGARNV
                     HVSVTHEGDHAVAVVIIEE"
BASE COUNT          912 a         1265 c         1015 g          828 t
ORIGIN      
        1 gggcaaaggc gtcattgcaa tggatgcgga gtagagggga ggcgctgaga cctgcggggc
       61 cccctccgat aacaagaact tctgtctgcc gcaatccctt caccttccct ctactccgca
      121 tccattgcaa tgacgccttt gccccgctgc gtctcgaccc gcatcccggt cttaagtggg
      181 gtaacgcagg tccggacatt cggaacgcca tcaacgacca tgaaacagga agaacatttt
      241 ccgatagcac agaagaaccc ccgcgtctgc tctttcacgg gggtaaccct gtagatcctg
      301 acaccattgg catgaagggc ggcggctatc ggttcgccct catagccctc catctcatta
      361 ccgtcgaagg tgaacttgac cttcttgcca tgcttgaact cgagaatcgg atgtttctcg
      421 atcagatcca taatcatgca tccctcctct gtatcgtggg cgtgacttga tctacagcca
      481 aaagtccatc ttccaccttc tccctttcat gggaatgttc tgcttttcac ttatgaacac
      541 gacagcaaat ataatacact tcattgttta gattgaccag agacttttat tcattttatc
      601 cgctgggagc acctgtatct ggagtcctga tatagagagc ccggacctcg ccgggatcga
      661 cggcctgtcc gaaaagcgcc ccgcccagca gggctactgt cccgcccctt acagaggcga
      721 aattggaaac cctgatattc atctctgaca gctcccggga tagagcctgg tcgtcatcag
      781 ccacggtcaa gaacccgctt ttcagttcga cagaactggc caggtcttcc agggagagga
      841 tccggggggg agagagaaag aacggggatc ggcgctcccc cccgagggcg gcgagataaa
      901 cctggcccct tcttgccctg acaaggggaa ggatcttttc tgacccgtcc aacaggggag
      961 ctcccagtgc catcagggag ctgacaggga ccaccttgcg gccaatcccc tctgacaggg
     1021 cacaggcata ggaaagtcct accctgatcc cggtgaagga acctggtccg acagtgacag
     1081 aaacagcatc cagtgaccag ggctccaggc ccgatctctc cagtacctct gaagtcagca
     1141 ggggaagcag ggaggcctgc ctcctgccgg ccagatagtt gacatcggcc ttaacggcac
     1201 cctccgagaa aaggccgacc ccggtccagc ccgtggaaca gtcgatccca agcactctca
     1261 tctgtcggcc tcctcaagtt cacatcctgc gccaaacatg aacctttcca gcatttcaca
     1321 cgccctgccc ccttcacact gcacctccag aaagcggagg ttctgggcgt cccggccgga
     1381 attcaggtcc tcaggcactg aaaacacgat cttccatacc tcggaaggtc tttccccgtc
     1441 ccagttctcc ggccattcaa caagaactat ggatccctca cgggaaaatt cttccaggga
     1501 gaactccgtc gaagcgttcc tctcaaccct gtacaggtca acgtgggtta tgggagtgga
     1561 tgccaggtac tcgttgatta tcgtaaagga ggggctcctt acatcccccc agcccgaccc
     1621 ggaggcaaaa cccctcacaa attccgtttt gcccgccccc agtgtcccgg aaaggaggat
     1681 caccagtcca gggaaggagt accttgaaag ggccgaagca agatgtctgg tttcctctgg
     1741 ccccctggat aggtaaaaaa aaccggcttt taccttgctg gacttctgac catggcaaaa
     1801 ggggatcatg gaaggacctt tccggctgcc gctgagatcc gggatagtac agaaggcact
     1861 tcaagagcaa tctctgtggc agtgataccg tctggaccct tccgctcctg aactgacccg
     1921 cctgcataac cgtgtacgaa agcggccgag gaggctgcgt cgaagatatt gatacccctt
     1981 gcaaggaacg ctcccaatac accggaaaga acgtcaccag atcctggaac ggaaagaccc
     2041 gccaagacat gggggttgac cctaaccttc tcactctccg acaccagcga acagggtcct
     2101 ttcagaaggg ctacaccgaa cctcctcgac agctccagaa ccgatctgta cctgcactgt
     2161 ttcacctccg aactgtccac tgagaggaga agcgctgctt cgccttcatg gggcgtcacc
     2221 acggtaccgg ctgccctggg atcaaggtca ctgcagcagg agatggcgtg aagggcatca
     2281 ccgtccagac agagcggacc atcccaatcc ttccatatcc tccggaccag gtcctggggt
     2341 acccttcccc tgtcaagccc gggaccgatc acaaccgcct tgaacctgcc cttccacatt
     2401 tcgacgatcc tgtcccaggt ctcaggagaa attgtaccgt cctcggtgca ggcctccatg
     2461 gggatgatct cgggataccg ggagaacatc ggcagggaac cagccggaac tgcggcgata
     2521 acgccgccgg aacctgccat cagagctccc agggctgcaa gaaggggtgc tccccggtat
     2581 ctctcgcttc cgccgatgac caagactata ccccgatccc ctttgtgtat gaagggggac
     2641 aggggaggaa gaaatccgga gacaataaaa gggtccacaa gtaccacttt ccctgaccgg
     2701 gggagaagaa gggtcgcctc gataccgagg tccaccgtct ccacttcgcc ccggaaactt
     2761 cttccgggca ttatttcatg tccgatcttc cgggcaagga tcgcgacagt aagatctgct
     2821 tccagggcat ccccttcgac ctcgcccgtc gatggatcga ctccgcttgg tatgtcaatg
     2881 gagactaccg gaacggtccc cctgacagcg cagatcaccc tccccgtctc gcccctgggg
     2941 gcgcctttcg agcctgaacc aaggagtgcg tcgacaagaa tatcctgccc cattgccagc
     3001 tctctcatcc cgacgtccgt cagctcttcc gatctcgcaa tgggacagcc tatccttttc
     3061 aggatcttca ggttcaaaga cccctcaggg gttaatttct cttctgcggc cgcaagaagc
     3121 acagtgacct tccggcctct tttcagaagg tgccttgcag ccgacaggcc gtctcccccg
     3181 ttgtttccgc ctccgcaagc caccagtatc ctggaggcgg ccgggaacct atcctccact
     3241 atctctgcga cacccctggc agcattctcc atcaggataa ttggggggat cccgaaatgg
     3301 tccacggcct ttctgtcggc aagtctgata tcgtcggcgc tgaaaagata caagtgatct
     3361 cactcctcta tgataactac tgctacggcg tgatccccct cgtgggtcac gctgacatgt
     3421 acattgcggg cccccaggga tcgaatcagt tccagggctg gaccataaag gataaggaag
     3481 ggtgctcctt cctttcgctc cagggatacg ttcttcaatc ccacgcggcc cagaccccag
     3541 ccgccggcct tggaaaaggc ctccttggca gcaaaggctg atgcaagatg ccttgtcctt
     3601 ccgcctctgg acgaagcata ctccctctcg ctttccgtga agaccttgtc gacgaacctc
     3661 gggtccagca gagccctctc aagtcggggg atgctgcaca tatccactcc aacacccttt
     3721 atcatctctt ccctccttgc ccaaaactga ttatatgata aaaacgggca ccttcagaag
     3781 ttccatccct gtcttggaat tctcccgaga aatgctgtcg ggaaggggag gaaggttata
     3841 ccacacggtt atccccggaa acaaaaaacc cctcccgcgt tcgcatggag gggtccatat
     3901 atacatgtat aaccgtcaga caacccttac acagaggctt ctgtcaggcc cccggcaaga
     3961 tatgaaaatg gtgggtggtg caggactcga acctgcgacc tcttccgcgt caaggaagcg
//
LOCUS       DAOPKQ010000124         3994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_24873_length_3994_cov_4.872049, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000124 DAOPKQ010000000
VERSION     DAOPKQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24873_length_3994_cov_4.872049"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..990)
                     /locus_tag="PK146_05135"
     CDS             complement(1..990)
                     /locus_tag="PK146_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HPE91660.1"
                     /translation="MRLITREEMRRVYSMEEAIEAMKLAFRLFSEGKAIVPLRTKIDV
                     SKSEGQALFMSAYVEETMSLGLKIVSVFPNNVKVGKPAVPATMVHMNPETGEVCAIMD
                     GTYLTQVRTGAAAGAATDLLARKNASVGALIGAGGQSSAQIEGMLAARKLKEVRIYDA
                     DGERAQKTAETQDRKWEKAGVRILATKSAEEALRDADVITTVTTARDPVFPAEAVKRG
                     AHINGVGSFKPDMQEIPPEIYLKANRVFVESREAVLSEAGDLIIPIKGGLFTEKIITG
                     ELGELVAGKVEGRKSDEEITLFKTVGIGVQDAVAAGEIYKRAVEQNVGTEFTF"
     gene            1216..1899
                     /locus_tag="PK146_05140"
     CDS             1216..1899
                     /locus_tag="PK146_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE91661.1"
                     /translation="MQDKCHPLLEPLVPVVKLLGAMLGKDCEILLHDVSKEAPFIVAI
                     ENGEVTGRDEKSRMTDLGYFLMTNPEAESVDFLANYPSEAENGKPLRSGVALIRDGNR
                     ELIGFLCINCDMTQGQVLKDMGEFLTTLHPLSFSDLQVERFSRVNDISAEEILESVRR
                     NLGKPLNYLSRSERMQCIEKLEEQGFFNLKGAIRMLARKMGKSRYTLYADLRAFHSGK
                     KLTDGPIND"
     gene            1983..>3994
                     /locus_tag="PK146_05145"
     CDS             1983..>3994
                     /locus_tag="PK146_05145"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPE91662.1"
                     /translation="MRRSIIRGFSVLVLLFLVGLSAAFAAGDPAIEYPETVRTAREIL
                     WKSVTSGGVNSGSVAVMDKGKIVFFEGYGPADRAEGKTVDRETRFNIGSTSKMFAAVS
                     ILLLVDEGRIRLDDPVVKHIPEFEMRDPRYRDITVRMLFNHSSGLPGSSFVFGYRPEG
                     DPHETLLDTLKESSLKHDPGAMGIYCNDGFTLAEMIVERISGKRYIDFLEERIFRPLG
                     MDHTGESIGVTGGKVARFYDPEGKKYPGEVAEVLGAGGLSSTVEDLCRFADSFMPGGM
                     HILSPSSIEEIMKVQPTAFSSSLEGQPFLEAFGWDYRVLPDFMELGYQMLAKSGGTLF
                     YSTNLQLLPSERMAVAVIFSGAGRAEEATYKVMKAMLQDRGLPVPGEDPLEKPVKAEP
                     IAESILPTSRYFMDESGFYRFEFDREKGLLDIYPVTGAGAGSSEEVRPVLSLIHNGGY
                     FHNLEKGDRFYLVSSHEGTFLVRSKIPRYGVDVPVYQMLETAESPLEMSVEMDGTVWL
                     VRNSPTFNSFTSYYLAIRSSAPKELPGYVTFSTPNRVVDPDYAEIAATGFRDQSSLRL
                     FRKEGEVRAGVLQFVFSREGTAKPLKEGTTLVAIGTEGENEWLKTVSEGILSFGFTEG
                     GRVVVLPGPGAPPLFDSVVDTGEVYVPEGCFIFLAGSPGDMIHISVR"
BASE COUNT          859 a         1041 c         1126 g          968 t
ORIGIN      
        1 tcagaaggta aactctgttc cgacgttctg ttcaacagcc cttttgtata tttctccggc
       61 agcaacggca tcctgaacac caatgccgac cgttttgaaa agggtgatct cttcgtcgct
      121 cttgcgaccc tctacctttc cggcaaccag ctctcccagt tcaccggtta tgatcttttc
      181 tgtgaagagc ccacctttga taggaatgat cagatctcct gcttccgaga ggactgcttc
      241 tcgggattcc acgaaaaccc tgtttgcctt cagatatatt tcaggtggga tttcctgcat
      301 gtcgggcttg aaggaaccta cgccgttgat gtgggcgccc cttttgacgg cctcggcggg
      361 gaagaccgga tcccttgcgg tcgtaacggt tgtgataaca tcggcatctc tcagggcttc
      421 ctctgccgac ttggtggcca gtatcctgac cccggccttt tcccatttcc tgtcctgggt
      481 ctctgcggtt ttttgagcac gctctccatc ggcatcgtag atcctgacct ctttcagttt
      541 cctggcagcg agcattccct cgatctgggc tgatgactgc cccccggcac ctatcagtgc
      601 ccctacagat gcgttcttcc tggcgagaag gtccgtggcg gcaccggcag cggccccggt
      661 acggacctgg gtgagatagg tgccgtccat aatggcacag acctctcccg tttcagggtt
      721 catgtggacc atggtcgcgg ggacggccgg cttgccgacc ttcacgttgt tgggaaagac
      781 cgacacgatc ttcagcccca gggacatggt ctcctccacg taggcggaca tgaaaagggc
      841 ctggccttca ctcttcgata catcgatctt ggtcctcagc ggcacgatag cttttccctc
      901 cgagaacagt ctgaaggcca gcttcatcgc ctcgatggcc tcttccatgg aataaacgcg
      961 ccgcatttct tccctggtaa tcaatcgcac acgatacccc tccctgtcgc tttactcttt
     1021 tcggcaaata tcatttctcc aattgagtct agcaatccga aaatggttag tcaacacatt
     1081 cttgtatgtt atatactttt gttgaattaa aagaaatgaa ccgttgcgct tgacagtatt
     1141 gatacattaa gcttttcctg gcagaaggcc tcgacatttc cgagaggccc tacctgacga
     1201 tcccggaggt gcaaaatgca ggataaatgt catcctcttc tcgaaccttt ggtccctgtg
     1261 gtcaagcttc taggtgcaat gttgggtaag gactgcgaga tcctccttca cgacgtctca
     1321 aaagaggccc cctttatcgt tgccattgag aacggggaag taactgggcg ggacgagaag
     1381 tccaggatga cggatctggg ctatttcctt atgacaaatc cggaagcaga gagtgtggat
     1441 tttttggcca attatccatc ggaagcggaa aacggcaaac cactgcgttc gggcgtggct
     1501 ttgatccgcg acgggaacag ggagctcata ggttttctct gcatcaattg cgatatgacc
     1561 caggggcagg tgctgaagga catgggcgaa ttcctgacga cccttcaccc cctgtccttc
     1621 agcgatctcc aggtggagcg tttttccagg gtgaacgaca tttctgccga agagatcctc
     1681 gaaagtgtcc gccggaacct cggaaaaccg cttaactatc tgagtcgttc ggaaaggatg
     1741 caatgtattg agaagctcga ggagcagggg tttttcaatc tgaagggtgc catcagaatg
     1801 ctggcccgca aaatgggtaa aagccgctac actctttatg cagacctcag ggcctttcat
     1861 tcggggaaaa aattaactga tggacccata aacgactgat cctgtggcaa acatctccag
     1921 gggggccatt tgcgatatat gtgaccgggc cggatccagg gcctgagaag ggggttggtt
     1981 tcatgagaag gtcgatcatt cgaggttttt ctgtccttgt cctgcttttc cttgtcggtc
     2041 tgtcagcagc ctttgccgcc ggggatcctg cgatagagta tcctgaaacg gtcaggaccg
     2101 cccgggagat cctctggaag tcggttacct ccggaggggt gaactccggg tccgtggctg
     2161 tcatggacaa gggaaagata gtgttctttg aggggtatgg ccccgccgac cgggcagaag
     2221 gaaagacggt cgacagggag accaggttca acatcggatc cacgagcaag atgttcgccg
     2281 ccgtctccat acttcttctg gtcgatgagg gaaggatccg cctggacgac cctgtcgtga
     2341 agcacatccc ggaattcgag atgagggatc cccgttacag ggacattacg gtcaggatgc
     2401 tattcaacca ctcctcgggg cttcctgggt cttcattcgt ctttgggtac aggcccgagg
     2461 gtgatcctca tgagaccctt ctggacacac tgaaggagag ttcacttaaa catgaccctg
     2521 gagcgatggg gatatactgc aacgacggct tcacccttgc ggagatgata gttgagagga
     2581 tctccggtaa acgttacatt gattttctcg aggaaaggat cttccgtcct ctcgggatgg
     2641 atcataccgg ggagagcatc ggggtgaccg gaggcaaagt cgcaaggttc tacgaccctg
     2701 aggggaagaa ataccccgga gaggtggccg aggtccttgg tgccgggggg ctgtcttcca
     2761 ctgtcgagga cctttgccga tttgccgaca gcttcatgcc tggaggcatg catatccttt
     2821 caccctcatc catagaggag atcatgaagg ttcagcccac agccttctct tcctcacttg
     2881 aggggcagcc tttccttgag gcttttggat gggattatcg cgttcttccg gactttatgg
     2941 agctgggtta ccagatgctg gcaaagagtg ggggtacctt gttctactct acaaatttgc
     3001 agcttctccc gtcggagagg atggccgtgg cggtgatatt ctccggggcc ggaagggccg
     3061 aggaggccac ctacaaggta atgaaagcca tgctgcagga caggggcctg ccggtccctg
     3121 gagaagaccc ccttgaaaag cccgtgaagg cagagccgat agcagagagc attttgccta
     3181 cctcaaggta ttttatggac gaatcaggtt tctaccgttt cgaattcgac agggagaagg
     3241 ggcttcttga catttatccc gttacgggag ccggggcggg tagcagcgaa gaggtgcgcc
     3301 ccgttctttc gctgatccac aatggcggat atttccataa cctggagaag ggagacaggt
     3361 tctacctggt ctcctcccat gaagggactt tcctggtgag gtcgaaaatc cccaggtacg
     3421 gtgtggatgt ccctgtctat caaatgcttg aaacggcgga gagcccgctg gaaatgtcgg
     3481 tcgagatgga cgggacggtg tggctggtcc gcaactcgcc gacattcaac agttttacca
     3541 gctattattt ggcgatacga tcttcggctc cgaaagagct tccaggttat gtcacttttt
     3601 cgacccccaa cagggtagtc gaccccgact atgccgagat agccgcgact ggtttccgtg
     3661 accagtcttc gctgaggctt ttccgaaagg aaggcgaggt cagggccggg gtcctccagt
     3721 tcgtcttctc aagggaaggg acggccaaac cgctcaagga agggacaacc ctggttgcaa
     3781 tagggacaga aggggagaac gagtggctta agacagtgtc agaagggata ctctcctttg
     3841 ggttcacaga aggaggcaga gtagtggtgc tacccggtcc cggggcccct cctttgttcg
     3901 acagcgtggt cgacacagga gaggtctatg taccggaagg gtgcttcatc tttctggctg
     3961 gaagccctgg agatatgatc catatttctg tacg
//
LOCUS       DAOPKQ010000125         3987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_24929_length_3987_cov_5.830875, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000125 DAOPKQ010000000
VERSION     DAOPKQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24929_length_3987_cov_5.830875"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK146_05150"
     CDS             complement(<1..363)
                     /locus_tag="PK146_05150"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPE91663.1"
                     /translation="MDEQHLIREAERIHRETLVLDAHFDLAMDLMDRTERGEEKVFRN
                     VFDGPFLEGGVDCVVSSIFVSQRFLPEQALRRGLDQVECLRREIELWPERLAFCTTVE
                     EIRNAAASGRLAIMLSFEG"
     gene            524..1321
                     /locus_tag="PK146_05155"
     CDS             524..1321
                     /locus_tag="PK146_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPE91664.1"
                     /translation="MTWRKELYVILFCLLYLSTVPTLPALAVPFWQEAARKHSDVAPR
                     EWGQEVTGVITRFDPQGGQLLALTFDACGGSVKGSGYDEELIGFLRREKIPATLFINA
                     RWIAANPDIFKELASDPLFEIANHGFEHKPLSVSGKEAYGIKGTGNAMEAAWEIEGGA
                     AAIYEVIGKRPLFFRSGTNHYDEVAVRIAGDLGQRVTGCSVNGDAGATNSARQVLEEL
                     LSARTGDIVLMHMNRPGGQTAEGVASAIEALKEKGFSFVRLFEVLGY"
     gene            1404..2744
                     /locus_tag="PK146_05160"
     CDS             1404..2744
                     /locus_tag="PK146_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91665.1"
                     /translation="MTRSLLAAVAALILSILSVAPAEPFKPLEVASLPDSVVISMSRG
                     FSRFPAISPCGTWVAWYSNSDTLTLEGDDNDVDLFARDLITGEMICISVSEDRGPEGV
                     GGDLFQPRGLSVSAGGDFVAFVAFRDEGRSDVMLRDVRARRTFLVSAGLDGGAGNGSS
                     LHPSISRNGRFIAFTSSASNLVEEDNNGFSDIFVHDMETGITERVSRGYPGDEADGQS
                     FLPSISWDGRMVAFTSSATNLVPGEKRQGPAVYVFDKATERTTLVSVSGDGRTASGDS
                     FSLPCSISGDGRNILFLLETTDLSAADSNSTEDLFVRDLPSGATIRASLGNNLTGSGQ
                     GAFSGALDFEGRVVVFASSSDHLVKGDVNSGSDIFLRDLQTRRVELISRASDGSPVFT
                     QSSDPVISGNGDIVAFSARTVDLVTGRGSENWQVFIREIRTGKTFWVSAPGTGP"
     gene            2767..3411
                     /locus_tag="PK146_05165"
     CDS             2767..3411
                     /locus_tag="PK146_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPE91666.1"
                     /translation="MDPGVFDIGLFLKGALLGFSIAAPVGPIGVLCIRRTLARGMVSG
                     FVSGLGAATADGIYGAIAAFGLASLTGFLVSVNLLMRIAGGIFLLWLGWKTISRKPQD
                     QHQEGDAPSGAASLAGNFVSTFFLTLTNPMTIISFTAIFMGVGLVNDGTRALQPLFLV
                     AGVFSGSAAWWLGLSAFFSLFRRHLTPVAMTWINRLSGAVLTLFGALVLFEIVV"
     gene            complement(3455..>3987)
                     /locus_tag="PK146_05170"
     CDS             complement(3455..>3987)
                     /locus_tag="PK146_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE91667.1"
                     /translation="ISGDRVSPMTWAGILLSFAGVVVISAGDFALGGKALFGDILALI
                     GAWFATLYFLTGRVVRAKVSLPVYTFICYGAAALVLLAVIVVTGLPFKGFSAGTWTAF
                     VGMALVSQLMGHSSYNWALRYLSASLVAVALLGEPIGATFLAWILFGETLTAAKLAGG
                     ALILAGIVIAVRGERI"
BASE COUNT          866 a         1143 c         1140 g          838 t
ORIGIN      
        1 tccctcaaag gagagcatta tcgccaggcg gcctgaggca gcagcattcc ttatctcttc
       61 cactgtggtg cagaacgcca ggcgctcagg ccagagttcg atctccctcc tgaggcattc
      121 cacctggtca agtcccctcc taagggcctg ttcagggaga aacctctggg agacgaatat
      181 ggaagagact acgcagtcaa ccccgccctc aaggaaggga ccgtcgaaaa cgttcctgaa
      241 gaccttttcc tctccccgtt ctgtgcggtc catcaggtcc atggcaaggt caaagtgcgc
      301 gtccaggaca agggtctccc gatgaattct ttctgcttcc ctgatcaggt gctgttcatc
      361 catatagatc ccctcctgtc cgataccttc ggagcgtagc acagcggcac tggaatagga
      421 aggatgtaaa ataaagagga tacgccggat ggcttcacgg cagtatccca atgaaagata
      481 tctatcgtcc cctgtaacgt attcgctgga tcggaggaag aacatgacct ggagaaaaga
      541 gctttacgta atattgttct gccttttata cctgtcaacg gtcccgaccc tgccggcgct
      601 ggccgttccg ttctggcagg aagcggctcg aaaacattct gacgttgcac ctcgtgagtg
      661 ggggcaggag gtaaccggcg tgatcacccg cttcgatccc cagggcggac aattgcttgc
      721 gcttaccttt gacgcttgtg ggggcagcgt gaagggaagc ggatacgacg aagaactgat
      781 cggcttccta cgcagggaga agatcccggc tacccttttc ataaacgccc gatggatcgc
      841 tgccaacccg gacattttca aagaactggc ttcagacccg cttttcgaga tcgcgaacca
      901 cgggttcgaa cacaagcccc tttcggtaag cggcaaggag gcctacggga taaaagggac
      961 aggcaatgcc atggaagccg catgggagat agagggcggc gcagcggcca tatatgaagt
     1021 tatagggaaa agaccccttt tctttcggtc cgggaccaac cactacgacg aagtcgcagt
     1081 caggatcgcc ggagacctcg gtcaacgggt cacaggttgc agcgtgaacg gagatgccgg
     1141 ggcgacaaat tcagctcggc aggttttgga agaactcctt tccgccagga ctggtgatat
     1201 cgtacttatg cacatgaaca ggcccggggg gcagactgcc gaaggtgtgg catcggcgat
     1261 cgaagccctg aaagagaaag ggttctcctt tgtccgtctc tttgaggtcc tcggttacta
     1321 aaaaaatccg taccgccaga tctttgggat gtcaaagcct ttatgagata atctatctca
     1381 ctttttcccg gagggttgcc aatatgacca gaagcctcct ggctgctgta gcagcgttga
     1441 tcttgagcat tttgtcagtc gctccggcgg aaccattcaa acccctggag gtggcctcct
     1501 tacctgatag cgttgtcata tccatgtcca gaggtttctc acgcttccca gccattagcc
     1561 cctgcggcac ctgggttgcc tggtactcca actccgatac cctgacactc gagggcgatg
     1621 acaacgatgt ggaccttttc gccagagacc tcattaccgg ggagatgatc tgtatatccg
     1681 tctctgaaga ccgaggaccg gagggagtgg gaggagacct ttttcagcca aggggccttt
     1741 ccgtcagcgc cgggggagac ttcgtcgcct ttgtggcctt tcgcgatgag ggaagatcgg
     1801 acgtaatgct gagggatgtc agggcaagaa ggaccttcct ggtatcggcg ggcctcgacg
     1861 gtggggcggg taacgggtcc tctctgcacc cctccataag caggaacgga cggttcatcg
     1921 ccttcacctc ctccgcgtca aacctcgtgg aggaggacaa taacggtttt tcagacattt
     1981 tcgtacacga catggaaacg ggaataacag agagggtatc cagaggatac cctggtgatg
     2041 aggccgacgg tcaatccttt cttccatcga taagttggga tggaaggatg gtggccttca
     2101 cttcgtcggc aacgaacctg gtgccaggcg aaaagaggca gggaccggcg gtctatgtct
     2161 tcgacaaagc aacggaacgg accaccctgg tatcggtatc cggcgacggc agaacagcca
     2221 gcggcgactc tttttccctt ccatgttcca tcagcgggga cgggcgcaac atcctcttcc
     2281 tattggaaac taccgatctg tccgctgcag acagcaacag cacggaggac ctcttcgtaa
     2341 gggaccttcc atcaggtgct acgatccgcg catctctcgg gaacaacttg accggatcgg
     2401 gccaaggtgc cttttcgggg gcactggatt ttgaaggcag ggtcgtggta ttcgcctcaa
     2461 gttccgacca cctggtcaag ggagatgtaa attccggatc cgacattttc ctgcgcgacc
     2521 tccagacccg gagagtggag ctcatatccc gggcaagcga cgggagtcct gtcttcaccc
     2581 agtcatcgga cccggtgatc agcgggaacg gtgatatcgt ggcattctcc gcccgcaccg
     2641 tggatctcgt gacaggaaga ggcagcgaga actggcaggt cttcattcgt gagatcagga
     2701 cgggaaagac cttctgggtc tccgcaccgg gtactggacc atgattgcag acaaggggtt
     2761 ccggtaatgg accccggggt gtttgacatc ggtctttttc taaagggagc cctcctgggc
     2821 ttctccatcg cagcaccggt aggccccata ggagtgctct gcataagaag gacccttgcc
     2881 aggggaatgg tatcgggatt cgtctcaggc cttggggccg cgacggcaga cgggatctac
     2941 ggggccattg cagccttcgg tctcgcttca ctgacagggt ttttggtgtc cgttaatctc
     3001 ttgatgagga tcgccggggg gatattcctg ctctggctgg ggtggaagac catctcaaga
     3061 aagccccagg accagcacca ggaaggagat gcaccctccg gggccgcctc cctggcgggg
     3121 aattttgtct cgaccttctt cctgaccttg accaacccca tgacgatcat ttccttcacc
     3181 gccatattca tgggcgttgg cctggtcaac gacggaacca gagccctgca gccgctcttc
     3241 cttgtggccg gggtcttcag cggctccgcg gcctggtggc tggggctgag cgctttcttc
     3301 tctctcttcc gccgacactt gacaccagta gccatgacct ggatcaacag gctgtccggc
     3361 gccgtcctga ccctcttcgg agcccttgta ctgttcgaaa tcgtggtttg agggtacggc
     3421 atcaacgccg cctgcacccc ttactcggga ccagctagat cctctccccc cttacggcaa
     3481 tgactatccc cgcaaggata agcgcccccc cggcaagttt ggccgcggtg agggtctccc
     3541 cgaagagtat ccaggcaaga aatgttgccc cgatgggctc tcccagcaag gccactgcca
     3601 caagactggc gctgaggtac ctgagggccc agttgtagct gctgtggccc attagctgcg
     3661 acaccagggc catgccgaca aaggccgtcc acgttccggc actgaatccc ttaaaaggca
     3721 gacctgtcac aacgatcaca gccagaagta ccagggcggc tgccccgtaa cagatgaagg
     3781 tgtagacggg gaggctgacc ttggccctga caacacgccc tgtgaggaag tagagggtcg
     3841 cgaaccaggc gccaataaga gcaaggatat cgccgaaaag ggccttacct cccagcgcaa
     3901 agtccccagc gcttatcact accactcccg caaagctgag caggatgcct gcccatgtca
     3961 tcgggctcac cctgtctccg ctgataa
//
LOCUS       DAOPKQ010000126         3963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_25146_length_3963_cov_2.591607, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000126 DAOPKQ010000000
VERSION     DAOPKQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25146_length_3963_cov_2.591607"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1290)
                     /locus_tag="PK146_05175"
     CDS             complement(58..1290)
                     /locus_tag="PK146_05175"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HPE91668.1"
                     /translation="MANEDRSFSISSEVGKLKRVMLHRPGKELERLTIDNKDELLFDD
                     ILWVEEAQKEHDAFADLLRDNGVEVVYFRNCLSHVLREESVRDPLLDEVLALEAIDIP
                     LVSALKAVMMEISPSDLAEMLIAGLTKMEAMEILPPCRGLVLRAMGDRDFLIRPLPNL
                     YFQRDPFTFIKNGVILSVMTFEARRKEPLYARYIFKNHPYFKGIEFLFGDHPRDTYPY
                     NIEGGDILILSDSVAAVGISQRTAAGTIQIVGKRIAERAGVKTLLAVDIPKTRAAMHL
                     DTVFTMVDHDAFTIYPEVKDFIRIWQLEYSSEGEITSVIEHQNLGECLRKVLGLDTVR
                     LIETGGGDPVAAARDQWNDGTNTLAIAPGVVVTYRRNVVSNRVLQENGIKVFEIKGAE
                     LGRGRGGPRCMSMPMMRD"
     gene            complement(1381..1752)
                     /gene="speD"
                     /locus_tag="PK146_05180"
     CDS             complement(1381..1752)
                     /gene="speD"
                     /locus_tag="PK146_05180"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPE91669.1"
                     /translation="MKQNGALGRHILVEAYDCDPSALDDILGLERSMKEAAEAAGATV
                     VESAFRKFEPHGVSGVLVISESHLTIHTWPEFGYAAIDLFTCGCNADPWKAFERLSSY
                     LGSSRTTSIEILRGHISGVDA"
     gene            complement(1749..2603)
                     /gene="speB"
                     /locus_tag="PK146_05185"
     CDS             complement(1749..2603)
                     /gene="speB"
                     /locus_tag="PK146_05185"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPE91670.1"
                     /translation="MERFICGRRSLKDASWVLQGIPLDMTVSRLHGTSLAPSSIRKES
                     RGLESFSFNLMSDLETLTFADIGDLGMVPGDLGRSLETVRYVSVGLFRRGFRVASLGG
                     EHLVTLPIMKAASEVYPDIAVVHLDAHADLRDQYHGMRENHATVMRRVAEECLECPSL
                     LYQFGIRSGTTEEYRWGLKNTSFYPLELTVPLLKVIPELQQRPVYLSLDIDIIDPGEA
                     PGTGTPEPCGLSSREVLDALYSMKGLDVVGFDIVEVSPPNDVNGITSILAAKLARECL
                     IMWGGGNR"
     gene            complement(2606..3454)
                     /gene="speE"
                     /locus_tag="PK146_05190"
     CDS             complement(2606..3454)
                     /gene="speE"
                     /locus_tag="PK146_05190"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPE91671.1"
                     /translation="MERRNTDIWFTEYQTKHLRLGLSIKSVLANIQTPYQHLLVVDTD
                     QYGRLMALDGAIQLTESDEFTYHEMLAHVALTSHPAPRRILIVGGGDGGTAREAVKHE
                     TVEEVVLVDIDQEVINASKQFFPSLSSGLEDPKVNVLVMDALEYIRGKSGKFDVVIVD
                     STDPVDFAEGLFREPFYRDVFRALNEGGSLVAQTESPFSDPDILTGAVSEMEKVFPIV
                     KVFWGAMPTYPTGMWTYTIGSKVHEPGEPRGQIPPETRYYSEEIHRAAFVLPPFLEEI
                     LQGRDR"
     gene            complement(3460..3945)
                     /locus_tag="PK146_05195"
     CDS             complement(3460..3945)
                     /locus_tag="PK146_05195"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HPE91672.1"
                     /translation="MLPVPELFTLVVGRGEGKKKLTAFDSALLDAGVGNMNLLRVSSV
                     LPPSCGFRHALKMPFGSLLPIAYGSITSDVPGETISASIGVGIPEDPASFGMIFEFSG
                     FCAGSEAAIKVEEMVREAFEMRSLDLKEVKVKAIDHVVKECGTVFAGCPLFFSYQSGK
                     V"
BASE COUNT          871 a         1122 c         1071 g          899 t
ORIGIN      
        1 ttctctactc ctcctgttga tagtgatctg gtatgtttta ccgggtacca gttgacttca
       61 atcccgcatc atgggcatgc tcatgcaccg ggggccgccc cggcctctcc caagctccgc
      121 ccccttgatc tcgaagacct tgatcccgtt ctcctgaagg accctgttgg aaacaacatt
      181 cctcctgtaa gttaccacta ctcctggagc gatagccagg gtgttcgttc cgtcgttcca
      241 ctgatccctg gcggctgcga ccgggtcacc tcctcctgtc tcgatgaggc ggaccgtatc
      301 tagtccaagg actttccgaa gacactcccc caggttctga tgctctataa cggaggttat
      361 ctccccttca gaagagtact cgagctgcca tatccggatg aagtccttca cttccgggta
      421 gatagtgaag gcgtcatggt ccaccatggt gaagaccgtg tcaagatgca tcgccgcacg
      481 ggtcttggga atatcgacgg ccagtaaggt cttgacgcca gccctttccg ctatcctctt
      541 gccgactatc tggatggtcc ccgctgcggt cctctggctg atgccgacgg ccgccactga
      601 atcggagagt atcaggatgt ccccgccctc tatgttgtag ggataggtat cccgggggtg
      661 atccccgaag aggaactcga tgcccttgaa ataggggtgg ttcttgaaga tgtaacgggc
      721 gtagagaggc tccttccggc gggcctcaaa ggtcatgacg ctcaatatta caccgttctt
      781 tatgaaggtg aagggatctc tctggaaata caggttggga agggggcgta tgaggaaatc
      841 gcggtccccc attgccctca gtaccaggcc cctgcagggc ggaaggatct ccatggcttc
      901 cattttggta agcccggcga tcagcatctc agccaggtcg ctggggctaa tctccatcat
      961 caccgccttc agggcggaga caagaggtat atcgatcgcc tcaagggcga ggacctcgtc
     1021 aagtagtgga tcccttacgc tttcttccct gagaacatgg gagagacaat tcctgaagta
     1081 gaccacttcc acaccgttgt ccctgagaag gtccgcgaag gcgtcgtgct ccttctgggc
     1141 ttcttccacc cagaggatat cgtcgaagag gagttcatcc ttgttgtcta tggtgagacg
     1201 ttccagttcc tttccgggcc tgtgaagcat gacccttttc agtttaccga cttccgacga
     1261 tatgctgaaa gatctgtcct cgttggccac cagaattacc ccctgtttcg gattatttgc
     1321 cccaatgaga agtataatcc atcggcggca aaaagctaaa aggcggcctg atcccttacc
     1381 ctaggcgtcc actccggaaa tgtggccccg caggatctct atggaggtcg tgcgggagga
     1441 gccgagatat gaggaaaggc gttcaaacgc cttccagggg tcggcattgc atccgcaggt
     1501 aaacaggtcg atggcggcgt agccgaattc cggccaggta tggatcgtga ggtgggattc
     1561 ggatatgacc agtacgccgc ttactccgtg aggttcgaat ttccggaagg ctgactccac
     1621 cactgtcgca ccggcggctt cggctgcctc cttcattgac cgttccagcc cgaggatatc
     1681 gtccagagct gagggatcac aatcataagc ttcaacaagg atatgtcttc caagagcgcc
     1741 gttctgtttc aacgattccc acctccccac atgatcaggc actctctggc cagcttggca
     1801 gccagaatgg acgtaatgcc gttcacatcg ttgggagggc ttacctctac gatgtcaaag
     1861 ccgacaacat ccaggccctt cattgaatag agagcatcaa ggacttcccg ggaggaaagg
     1921 ccacatggtt cgggtgtacc ggtcccggga gcctctcctg ggtcaatgat atctatgtcc
     1981 agcgaaagat agacaggtct ctgctgcagc tcggggataa ccttcagaag ggggaccgtc
     2041 agttcaaggg gataaaagga ggtattcttt aacccccaac gatattcctc ggtcgtgccg
     2101 gacctgatcc cgaactgata cagaagagag gggcattcaa gacactcctc agctacacgc
     2161 cgcatgaccg tggcatggtt ctctctcatg ccgtggtact ggtccctcag gtctgcatgg
     2221 gcgtcaagat gaacaaccgc gatgtcggga tagacttcag atgcggcctt catgatcggg
     2281 agagtgacaa ggtgctcccc tcccagggag gcaaccctga agcctctacg gaagagaccg
     2341 accgaaacgt acctgacagt ctcaagggat ctcccgaggt cacccggtac cattccaaga
     2401 tcgccgatgt ccgcaaaagt caaggtttca agatcactca tgaggttgaa actgaaggac
     2461 tcaagacccc tggactcctt cctgattgag gaaggggcaa gggatgttcc gtgaagacgt
     2521 gacacggtca tatccagggg aatgccctga aggacccagg aagcatcttt tagggaccgg
     2581 cgtccgcata tgaacctttc caactctatc ggtcccgtcc ctgcaggatc tcttccagaa
     2641 aaggcgggag aacgaaggcc gccctgtgga tctcttcaga atagtacctg gtctcgggcg
     2701 gtatctgccc cctgggttcc ccgggttcat ggaccttgga gcctatcgta taggtccaca
     2761 tacctgtcgg ataggtaggc atggcgcccc agaagacttt gacgatgggg aagaccttct
     2821 ccatttcaga gacggcacct gtcagtatgt cagggtcaga gaagggggat tccgtctggg
     2881 ctacaagtga tccaccttcg ttaagagccc tgaaaacgtc cctgtaaaag ggttccctga
     2941 aaagcccctc ggcaaaatcg accggatcgg tgctgtcaac gatgaccacg tcaaactttc
     3001 cactctttcc cctgatatac tccagggcgt ccatgacgag tacgttgacc ttcgggtcct
     3061 ccagtcctga gcttagggag gggaagaact gtttcgatgc gttaataacc tcctggtcga
     3121 tgtctaccag gaccacttcc tcgactgttt catgctttac cgcttccctc gcagtccctc
     3181 cgtcaccgcc gcccacgatc agtatgcggc ggggggccgg gtgggacgtc agggccacat
     3241 gggcgagcat ctcgtgatag gtgaactcgt cactctcggt gagctggatc gccccgtcga
     3301 gtgccatcag cctaccgtac tggtcggtat cgaccacgag aagatgctgg taaggggtct
     3361 ggatatttgc aaggacgctt ttaatgctca gcccgagcct gagatgtttt gtctgatatt
     3421 cggtgaacca tatgtctgtg tttcttcttt ccattcccgt cagacctttc cgctctggta
     3481 agagaagaag agaggacagc ctgcgaatac cgtcccgcat tcctttacaa catgatcgat
     3541 cgccttgacc ttgacctcct tgaggtccag ggaacgcatt tcaaaggctt ccctgaccat
     3601 ctcctcgact ttgatggcag cctctgatcc ggcgcagaac cctgagaact cgaatatcat
     3661 accgaacgat gcgggatcct ccggtatgcc tacaccgatc gacgcagaga tggtctcccc
     3721 gggaacatcg ctggtgatcg aaccgtaggc gatgggaagg agagacccga agggcatctt
     3781 cagggcgtgt ctgaaaccgc agctcggcgg gagaacgctg cttacgcgaa gaaggttcat
     3841 attacctact cccgcgtcaa gaagagcaga atcaaaggcc gtaagcttct tcttcccctc
     3901 tcctcggcct actaccagtg tgaaaagttc gggcacgggc aacatgtcaa ggcaagcctc
     3961 cta
//
LOCUS       DAOPKQ010000127         3906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_25650_length_3906_cov_4.397040, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000127 DAOPKQ010000000
VERSION     DAOPKQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25650_length_3906_cov_4.397040"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..951)
                     /locus_tag="PK146_05200"
     CDS             complement(115..951)
                     /locus_tag="PK146_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPE91673.1"
                     /translation="MLTKVWGTEFAALDLETTGLDPLNDRIVEAAAVIFRGSEVIDTF
                     ETLVNPGVRITSALTAIHGITNSMVKGAPAHAEAVSTLAGFVGERPLVIQNAPFDMGF
                     IDTVRRESFSGPLDNDLFDTCRLAPVIFPGLPSYSLGSLARALGVPKGRSHRALDDSL
                     AAMGIFLKCMEKIDPAGNIEYERFERDYSFRSLLALERSPALLQWPSGFEAIREAFEV
                     SGKIFIVYRGGNGSVTERLIEPTGLVRIGGRIMLEAWCSLRGESRTFRFDRILKAWKQ
                     EE"
     gene            1080..2042
                     /locus_tag="PK146_05205"
     CDS             1080..2042
                     /locus_tag="PK146_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91674.1"
                     /translation="MKTVIESFFNSLKTGEPCSFKGLTAFPVFSALPEAVDFVTLTEA
                     FREDWIEIRELTQGGSVPELLVINKSDRNILILDSEELQGAKQNRVLNITVLVTAWSK
                     VVVPVSCTERGRWHYISNVFRDSGTMASRSVRFSKNRSVGESLRRDNSFRSDQMEVWD
                     KVDELSMRSGTMSRTHAMRDVFVDMDEKLRSFEKAFPLSEGQRGVIFFAGKEPAGFEY
                     VSDPRAFRDLFPKILRSYAVEAPPGEAKTAKALSGDQAMAFVGKVLSAETTVKHSAGR
                     GEDIRFSGDGVTGSALAYEGEILHAVFFPDEKEGRPVIRRRPVS"
     gene            complement(2217..3503)
                     /locus_tag="PK146_05210"
     CDS             complement(2217..3503)
                     /locus_tag="PK146_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HPE91675.1"
                     /translation="MTGLLFRLFLVISESLNFCLLYLFATSGFGVAGFVNPWSFLTVS
                     ASVAAVNLAMRRGSFRYLAIAVANVTVFVAALRLGRDPAALFSAPGNPAAVAGFWATW
                     MLLLAAGGRAAFLSWTRDPRRYGLFDINIAALFLMLLLASSFGIRLPGVHVLLFLAIL
                     SNILMLFFGSRGESSGKGRWRVLLGLAALMVPVWLIGNSDALSVLEQPAGSLIEFSKP
                     FFRLLLQGLTAVLLVIFRLMKLAPRKQIQTGAGEGSAVAEEGLLLPGETGPFMQALLC
                     GMTVAVLVLAALLVLYMLWMLVSFLLQRRTGSVSGGTWSRPSFRAFLVAMYRALKRLA
                     LMASIFLPLPITAERAYGFLLFWGKHRGSPRKPDETAYEYLERLLERFPDRERELSRI
                     TGSFVRHRYSREGKVFERGLKWYLLKLYVRVPFLKA"
     gene            complement(3500..>3906)
                     /locus_tag="PK146_05215"
     CDS             complement(3500..>3906)
                     /locus_tag="PK146_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91676.1"
                     /translation="PSRETPGQGAGAHRDLRLCITLDGDSFDTPGPSMEEALSLAATL
                     AVWADRKRIPFGLVSNVSRCGMSGPVSIPVGSGPTQVRLALEALARAELPVALPFGEF
                     LRKESRLLPWGTTVISIRGEIPAEALSEVTPS"
BASE COUNT          868 a         1210 c         1090 g          738 t
ORIGIN      
        1 gccggtagct caattggata gagcatcgga ctacggatcc gaaggttacg ggttcgactc
       61 ctgtccggcg cgccacttaa ataacggaag gccccactca aagtggggcc tttttcattc
      121 ctcctgcttc caggccttca ggatccggtc gaacctgaag gtccggctct cccccctgag
      181 tgagcaccag gcctcgagca tgatcctgcc tccgatcctt actagccccg tgggctctat
      241 aagcctctca gtcacagagc cgttaccacc ccggtagact atgaagatct tccccgagac
      301 ctcaaaggcc tcccttatgg cttcgaaacc ggaaggccac tgcagaaggg cggggcttcg
      361 ctccagggcc agaagcgatc tgaaggagta gtccctttcg aacctctcgt actcaatatt
      421 ccctgccgga tcgatctttt ccatgcactt caggaatatt cccatggctg ccagggagtc
      481 gtccagggcc cggtggctgc gtcccttcgg cacaccaaga gcccgggcca ggcttccaag
      541 gctgtagctg ggaagccccg ggaagatcac cggggcaagg cggcaggtgt cgaaaagatc
      601 gttgtccagt ggtccggaaa agctttcccg gcgtacggta tcgatgaagc ccatatcaaa
      661 gggggcgttc tggatgacca ggggcctctc tcccacgaag ccagccagcg tcgacaccgc
      721 ctcggcatgc gccggggcgc ctttcaccat gctgttggta atgccgtgga tcgcggtaag
      781 ggcagaagtg atcctcactc ccgggttgac cagggtctcg aaggtgtcta ttacctcgct
      841 ccccctgaaa ataacagccg ctgcttcgac gatcctgtcg ttaagggggt caagtccggt
      901 tgtctccaga tccagcgcgg cgaactccgt gccccaaacc tttgtcagca agatcaccct
      961 cgatctcaat ttctttacta caggatatat caatttacat aaccaggcca aacctgtttt
     1021 gataccacat ccaaaatgtc gtctgccctc cctagaatga gatccaggag gtggtagaaa
     1081 tgaagaccgt catagagagt ttcttcaata gcctcaagac aggcgagccc tgcagcttca
     1141 agggacttac ggccttcccg gttttctccg ccctcccgga agcagtggac ttcgtgaccc
     1201 tcaccgaagc cttcagggag gactggatcg agatccggga attgacccag ggaggctccg
     1261 tgccggaact gctcgtcatc aacaagagcg accgaaacat cctcatcctc gacagcgaag
     1321 agctccaggg tgcgaagcag aacagggtgc tcaacataac cgtgctggtg acggcctggt
     1381 ccaaggtggt ggtccccgta agctgcaccg aaagggggag gtggcactac atctcgaacg
     1441 tcttcaggga ctctggcacc atggcgtccc ggtccgtgag gttctccaag aaccgttccg
     1501 taggggagag cctcaggaga gataacagct tcaggtccga ccagatggag gtctgggaca
     1561 aggtagacga gctctcaatg agatccggca ccatgtcacg gacccatgcc atgcgggacg
     1621 tattcgtgga catggacgaa aagctccgat ctttcgaaaa ggccttcccg ctctccgaag
     1681 gccagagagg ggtgatcttc tttgccggaa aggaacctgc cggtttcgaa tacgtctctg
     1741 atccccgggc cttccgggac ctcttcccga agatactccg aagctacgcc gtggaagccc
     1801 cgcctggaga agcaaagaca gcaaaggcac tttcagggga tcaggccatg gcctttgttg
     1861 gaaaggttct ttcggctgag accacggtaa aacactctgc cggcagggga gaggatatca
     1921 ggttttcagg cgatggcgta acaggttccg ccctggccta cgagggcgag atccttcacg
     1981 ccgtcttctt ccccgatgag aaagagggca ggcccgttat ccgcaggaga cctgtcagtt
     2041 agcgggaagg ccccgtgcga catctacctt gaattccgcg accgcttcga ccttccgaaa
     2101 aattacgggg cgggaatcct tccccgcccc tgcatttttt ctcctgccgc cttgttttct
     2161 gtttttcttc ccgttcaccg ttgaccgttg acgcttgtat tattcttgcc ccgcccctaa
     2221 gctttcagaa acggcaccct cacatacagt ttcaataagt accatttgag gcctcgctca
     2281 aagacctttc cctccctgga gtacctgtgg cgcacgaaac ttcccgtaat gcgcgacaac
     2341 tccctttccc ggtcgggaaa cctctccaga agcctttcca ggtattcgta ggccgtctcg
     2401 tctggtttcc tcgggctccc tctgtgtttc ccccagaaga gtaggaaacc gtaggcccgt
     2461 tctgctgtaa tgggcagcgg caaaaatatg ctggccatga gggccagccg ctttagcgcc
     2521 cggtacatgg caaccaggaa ggccctgaaa gaaggcctcg accaggtccc acccgagaca
     2581 gagcctgtcc tcctttgaag cagaaagctc accagcatcc acagcatgta gagcaccagc
     2641 agcgcggcaa gaaccagtac cgccacggtc atgccacaga ggagagcctg catgaagggg
     2701 cccgtctccc cgggcaacag aagcccctct tccgcaacgg cagagccttc acctgctccg
     2761 gtctggatct gtttgcgggg tgccagcttc atcaacctga agatcaccag gagcactgcc
     2821 gttagcccct gaagaagaag cctgaaaaag ggcttgctga attcgatcaa cgaccccgcg
     2881 ggctgctcca gcacagaaag tgcgtcagag ttcccgatca gccacaccgg caccataagt
     2941 gctgccaggc caaggagcac gcgccagcgg ccctttccgg aactctcccc cctggagccg
     3001 aaaaagagca tcagtatgtt cgaaaggatc gccaggaaca gcagtacatg cactcccgga
     3061 agacggatcc cgaaggatga ggccagaagc agcatcaaaa acagcgccgc gatgttgatg
     3121 tcgaagagcc cgtatcgacg tggatccctg gtccaggaga ggaaggctgc ccgtccgccc
     3181 gcggccagaa gcagcatcca tgttgcccaa aatcccgcca ccgccgccgg gttgccgggg
     3241 gcagaaaata gcgcggccgg atcacgcccc aggcgcaggg cggcgacgaa gacagtaacg
     3301 ttcgcaacgg ctatcgccag gtaccggaag ctccctcgcc tcatggccag gttcaccgcc
     3361 gccacagagg cagacacggt cagaaaggac caggggttaa cgaaccccgc caccccgaac
     3421 cccgatgtgg caaagagata gagcaggcag aaattcagcg actcgctgat gaccaaaaac
     3481 agcctgaaca ggagacctgt catgacggcg tcacctctga cagagcctcg gcagggatct
     3541 cgcctcggat ggagatcacc gtagttcccc agggcagaag cctcgactcc ttcctcagga
     3601 actccccgaa gggaagggct acgggaagct ctgcccgggc gagcgcctcc aaagccagcc
     3661 gcacctgggt ggggcccgac cctacgggga ttgatacggg acctgacatg ccgcatcggg
     3721 agacgttcga tacaagcccg aaggggatcc tcttcctgtc tgcccacacg gccagggtag
     3781 ccgctaggga cagggcttcc tccatcgagg gtccgggcgt gtcaaagctg tccccgtcca
     3841 gggttatgca tagtctcagg tcgcggtgtg ctccagcacc ttggccagga gtttcccgtg
     3901 acgggc
//
LOCUS       DAOPKQ010000128         3900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_25711_length_3900_cov_4.024447, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000128 DAOPKQ010000000
VERSION     DAOPKQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25711_length_3900_cov_4.024447"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PK146_05220"
     CDS             complement(<1..1215)
                     /locus_tag="PK146_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HPE91677.1"
                     /translation="MKKAATILLAISLTIAAGAAFAVEAEEVMAKNGMVSSAHELASR
                     AGVEIMQKGGNAVDAAIATMLALNVVEPNASGIGGGGFMVIRNAKTGEVVFLDYREVA
                     PASATKDMYASQQSKDENWSTVGGKAVGVPGQLMGIFAALENYGTMTFAEVAVPAIRL
                     AEEGFEVHPMQTGIIADNYEKIAKNNDLGDLAFFEDGLPIEAGKILKQPRLAEAFHLI
                     AEHGVKAMTEGPLGESVVAAVNRAGGKMSMDDLKNYQVKIGDPAYGTYRGYHIYSTPP
                     ASSGGTHIVQLLNILENFPISSMKHNSPQYLHTLAEAMKLIFADRGKYMADTAFVDVP
                     LRGLTSKEYARELARKIWVYEVMQEVQPGDPWPYNGGDEIAFLGGGGNKHISTSHFSV
                     VDKEGNIVASTNT"
     gene            complement(1295..2437)
                     /locus_tag="PK146_05225"
     CDS             complement(1295..2437)
                     /locus_tag="PK146_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPE91678.1"
                     /translation="MLSFNKGTRASAAFLLAFALVLAAFTGKNFLEMRLPDRVVPAPG
                     FTSHMLSEWFPNISGTPVDTPVFTQDSGVPGGSVLIMGGTHPNEPAATMTAVIFLERA
                     NVSRGKLFVIPFANMSAFGHNSPQEASPQRIHFTLPDGSVRSFRYGSRATNPVHQWPN
                     PDIYIHEASGQQLSGSEMANLNRAHPGVEDGPLTQRLARGLLELLLREEIDLAFDLHE
                     ASPEYPVVNAIVAHEKSMMLAAMVTMELEARGVPIRLEPSPKNLRGLTHREWGDATAT
                     MPILMETGNPSQGRLRGRTDEDLVMTGQDKFYAKAAELGRLFIPYVTDQPMDLRVARH
                     TGSILTFLETLEYLDPEIAITVEGVPSYGEILEEGVGAFLSPVPDQ"
     gene            complement(2451..2594)
                     /locus_tag="PK146_05230"
     CDS             complement(2451..2594)
                     /locus_tag="PK146_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91679.1"
                     /translation="MTIVYRAIALFILVFVLRCMFREKDFWKQVTATMVVIPLVLRIL
                     LIK"
     gene            complement(2608..3894)
                     /locus_tag="PK146_05235"
     CDS             complement(2608..3894)
                     /locus_tag="PK146_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPE91680.1"
                     /translation="MSWFWPEGFYTLAMITAFGFGAFALKLPIAVAMGLAAVTGSLLG
                     GAGIPLRHLVEGMFGYIDTILIIATAMIFMKTIEATGLLESVAAWMIRKFRSFPAVLS
                     LGIVFLIMVPGMITGSSTAAVLTTGALVAPVMIKLGVPTLQTAAAIAMSAIYGMIAPP
                     INLPAMIIGGGIDMPYVGFGLPLLICTVPLAVFTALWLVYPSLRKKSHDPALEEELSR
                     MARTPLTARLFLPMAALVVLLGGERMFPEVWPPLGMPLSFLIASAVGIFSGVKWNIPE
                     TVTEAINDALPVMGILMGVGMFIQIMTLIGVRGFVVVSALALPAWLLYVGIATSMPLF
                     GAVSAFGSASVLGVPFLLALLGRNEILVGSALSLIAGLGDLMPPTALAGIFAAQVVGE
                     KNYFRVLRFCVVPGLLTAVWGIAVILGAQYIAGILY"
BASE COUNT          832 a         1139 c         1118 g          811 t
ORIGIN      
        1 ggtgttggtg gaggctacaa tgttcccctc cttgtcaacg acggagaagt ggctcgtgga
       61 gatatgcttg ttgcctcctc cgccaaggaa cgcgatttcg tcgcccccgt tgtagggcca
      121 tggatcaccg ggctggacct cctgcattac ctcgtagacc catatctttc gggccagctc
      181 ccttgcgtat tccttgctcg tcaagccccg cagcggtacg tcaacaaagg ccgtatcggc
      241 catgtacttg ccccggtcgg cgaagatgag cttcattgct tcggcgagag tatgcaggta
      301 ctggggagag ttgtgcttca ttgagctgat ggggaagttc tccagaatgt tcagcaactg
      361 gacgatgtga gtcccgccgc tcgaagcagg aggggtggag tagatatggt agcccctgta
      421 ggtcccgtag gcaggatccc ctatcttgac ctggtagttc ttgaggtcgt ccatcgacat
      481 tttccctccg gcacggttga ccgctgcgac aacagattcg cctaagggcc cctcggtcat
      541 agccttgacc ccatgttccg caataaggtg gaaggcctcg gcaagtctgg gctgcttaag
      601 gatcttcccc gcctcaatgg ggagtccgtc ctcgaagaag gcaaggtcac ccagatcgtt
      661 gttctttgct atcttctcgt agttgtcggc aataataccc gtctgcatcg ggtggacctc
      721 gaagccctcc tcggcaagcc tgatggctgg taccgccact tcagcgaagg tcatggtccc
      781 gtagttctca agagcggcaa agatccccat caattgtccg ggaactccga cagccttccc
      841 tccaacggtg gaccaattct catccttgga ctgctgggac gcgtacatgt ccttcgtagc
      901 cgatgcggga gcgacctcac gataatcaag gaacaccact tcaccggtct ttgcgttcct
      961 gatcaccatg aaaccccctc cgcctattcc cgaggcgttg ggctccacta cgttcagggc
     1021 gagcatggtt gctatggccg catcaactgc gttaccgccc ttctgcatga tctcaacccc
     1081 cgccctcgac gccagttcat gggccgagga aaccatcccg ttctttgcca ttacctcttc
     1141 ggcctctacc gcaaaagccg cacccgcagc tattgtcagc gatattgcca gaaggatcgt
     1201 cgctgccttt ttcatcttgc ttttcctccc ttcccttcag tgatagttgc tgcctctaaa
     1261 aagatcattc cgcctccaga ggcggagaga acccctattg gtccggtaca ggcgacagaa
     1321 aagccccgac gccttcttcc agtatctctc cgtaggaggg tacgccctcc acggtgatcg
     1381 ctatctcggg atccaggtat tccagcgttt ccaggaaggt gagaatcgat ccggtatgcc
     1441 gtgcgacacg gagatccatc ggctgatcgg tcacataggg tatgaagagc cttcccagtt
     1501 ccgccgcctt ggcatagaac ttgtcctggc ctgtcatgac aaggtcttcg tcagtcctcc
     1561 cgcgaagacg tccctggctt gggttgcctg tttccatgag aatgggcatg gtggctgtgg
     1621 catcgcccca ctcccgatgg gtaagccctc tgaggttttt tggcgagggc tcgagcctga
     1681 tgggaactcc ccgggcctcc agttccatgg tgaccatggc ggcaagcatc atgcttttct
     1741 cgtgggcaac aatggcattg accacagggt actcggggct cgcttcatga aggtcgaatg
     1801 ccagatctat ctcttccctg agcagaagtt caaggagccc cctggcaagg cgctgggtga
     1861 gagggccgtc ctccacaccg ggatgagccc ggttcaggtt cgccatttcg cttcctgaga
     1921 gctgctgtcc cgatgcctcg tgtatataaa tgtcggggtt gggccactgg tggacaggat
     1981 tggtcgccct cgatccgtaa cggaaagacc tgacgcttcc atcgggaagg gtgaagtgga
     2041 tcctctgcgg ggaggcctcc tggggcgagt tgtggccgaa ggcggacatg ttggcaaagg
     2101 gtatcacgaa aagcttcccc ctgctgacat ttgctctttc aagaaagatg acagctgtca
     2161 tggtcgccgc cggttcgttc gggtgagttc ctcccatgat aaggaccgac cctccgggga
     2221 ctccgctgtc ctgggtgaag acaggggtgt caacaggtgt gcccgatatg ttcgggaacc
     2281 attcggagag catgtgcgaa gtgaaacccg gggccggaac gaccctgtcg ggcagtctca
     2341 tctcaaggaa gttctttcct gtaaaagccg cgaggacgag cgcgaatgca agcaaaaagg
     2401 cggcggaggc gcgggttcct ttattgaaag acagcatcgt cgccaccacc ctacttgatc
     2461 agaaggatcc tgaggaccaa cggaatgacc accattgtcg cggtgacctg tttccagaag
     2521 tccttctccc tgaacatgca gcggaggaca aaaaccagta tgaaaagggc tatagccctg
     2581 tagacgatcg tcatataccc ttacctccta gtagaggatc cccgcgatat actgggctcc
     2641 caggatgacc gcgatccccc agactgctgt caacaggccc ggaacaacgc agaagcgcag
     2701 gaccctgaag tagttctttt caccgacgac ctgggcggca aagatgcctg ccagtgctgt
     2761 agggggcatc aggtcaccca gtcctgcgat caggctgagg gcagaaccga caagaatctc
     2821 gttcctgccc agaagcgcca gcagaaatgg gacccccagc accgaagcgg aaccgaaggc
     2881 cgatactgcc ccgaaaaggg gcatcgaagt ggctatgccc acgtagagaa gccatgcagg
     2941 cagtgccagt gctgaaacga cgacgaagcc cctcactcct atgagggtca tgatctggat
     3001 gaacatgccc acacccataa ggatccccat taccggcaga gcatcgttga tcgcctcggt
     3061 gacagtctcg gggatgttcc atttgacccc tgagaagatg cccacagcag aagcgataag
     3121 gaatgagagt ggcatcccca gggggggcca gacctccggg aacatccttt ctccgcccag
     3181 gagcactacc agcgccgcca ttggaaggaa aagcctggct gtcaacggtg tccgggccat
     3241 tcttgagagc tcctcctcaa gggcagggtc atgactcttc tttctgagcg aggggtagac
     3301 cagccagagg gcggtgaaga cggccagcgg gacggtacat atcagcagcg gcaacccgaa
     3361 gcccacgtag ggcatgtcta tgccgccgcc tatgatcatg gcaggaaggt tgatcggcgg
     3421 agcgatcatt ccgtagatcg ccgacatcgc aattgccgct gcggtctgca gggtcggaac
     3481 cccgagcttt atcataaccg gcgccaccag ggctccggta gtgaggaccg cggcggtgga
     3541 ggatccggtg atcatcccgg ggaccattat caggaagact attccaaggg acaggaccgc
     3601 cggaaatgac ctgaacttgc ggatcatcca tgcggctaca gattcgagga gccccgtggc
     3661 ctcgatggtc ttcatgaaga tcattgccgt agctattatc aggatcgtgt cgatgtagcc
     3721 gaacatgccc tccacaaggt ggcgcaaggg gataccggcg cctcccagga gagagcccgt
     3781 aacggccgca agacccatgg caacggctat tggcagcttc agggcgaagg cgccgaaacc
     3841 gaaggccgtt atcattgcca gggtgtagaa accttccggc cagaaccagc tcatggccga
//
LOCUS       DAOPKQ010000129         3869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_25979_length_3869_cov_4.782905, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000129 DAOPKQ010000000
VERSION     DAOPKQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25979_length_3869_cov_4.782905"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..592
                     /locus_tag="PK146_05240"
     CDS             437..592
                     /locus_tag="PK146_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91681.1"
                     /translation="MKERKATQQRGIHDLVMRSPGMRPRPVDLDFDFEGLPMAWKEAR
                     EALERKR"
     gene            complement(600..1814)
                     /locus_tag="PK146_05245"
     CDS             complement(600..1814)
                     /locus_tag="PK146_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE91682.1"
                     /translation="MITPGLVETVFSAASMTRWNDHARPAQFTELAKQAHKMAIAWVI
                     ARCEEDQGGDPDWVRLVEGGLFEFFQRVVLTDIKPPVFHRMMERHGERLNRLVLDKLA
                     RHLESLGGGFFERFRKYLKEPDFSGREKRILRASHYLATNWEFRMIYGQNRELSSVEE
                     TRRDIEGQLEDHIDLAGVREMIMRRTMTDKGLYAFIDLCGQLRFQVRWAQLPRVPATS
                     VLGHLFMAASLSYFVSLENGGCPRRLRNAFFGGLFHDLPEVLTRDIISPVKRSVEGLE
                     ELIKEYENESMETRLLPLIPEGWRKELRYLTSDEFSNRARDPVTGNIISGLTLSEMNS
                     NYNCEEWDPVDGTVIDACDKLAAMVEAATSIRYGICPPALEEGRRDIFEKYSNTVIGT
                     VDLGSLFGAFLE"
     gene            1906..2394
                     /gene="ybaK"
                     /locus_tag="PK146_05250"
     CDS             1906..2394
                     /gene="ybaK"
                     /locus_tag="PK146_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HPE91683.1"
                     /translation="MKKTNAVRLLESRGVAFELWNYESDPDDVSARAVAAKIGLAPEM
                     IFKTLVARGDRTGVMLACVGGDSELDLKALAAASGNKKVEMVHMREIQSLTGYIRGGV
                     SPVGTKRPYPVYIDESAFAFDRICVSGGARGVQIFIDPRVLAAEVEALPAPIAKQDLP
                     ES"
     gene            complement(2363..3115)
                     /locus_tag="PK146_05255"
     CDS             complement(2363..3115)
                     /locus_tag="PK146_05255"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPE91684.1"
                     /translation="MRNGSKLSERTSKALRIIGQAPEPMGAGSLARSMTERGDPVSMA
                     TAGRILGELDGGGYLEKRSNRGRVLSAKGREHLERLEALGKGETLARALLEELKTHSP
                     GDLLDILVARRAIERETARLAALKASTEDLEQIRAFAEILDGDRSQVPSISVIDRLFH
                     EAIAKAGKNKVLLATLKLVRQDVHTQQIFTFIRQQEGSRLGGDHMPIYLAIAGRDPGD
                     AEKAMLRHIDNIVRDVEHYCRGANSQADPALR"
     gene            complement(3137..3844)
                     /locus_tag="PK146_05260"
     CDS             complement(3137..3844)
                     /locus_tag="PK146_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91685.1"
                     /translation="MNALLVEDLNVHYGTIHAVQGVSFSVNEGEIVSIVGSNGAGKST
                     IMWTLAGVVPPSGGRILIKGNPIPSKPHEIVARGLALVPERRRLFSALTVKENLVMGA
                     YRRKDEESVQAEMERCFQLFPILRERLNQRSGTLSGGEQQMLAISRALMSDPGILLLD
                     EPTLGLAPLIVEAVMNTVIRIRNEGVTVLMVEQNATQALEISDRGYILEAGLFVKTGP
                     GPDLAMDPEIRRAYLGG"
BASE COUNT          746 a         1136 c         1118 g          869 t
ORIGIN      
        1 tcctgatgcc tgacgcctgc ttttctgacc tttcatttta cttctcacgt ctgacatctc
       61 acgatctttt tcccttgacg cccatccggg aaagtggtat aaaacttccc aatacagaaa
      121 agcggaggca tcgatgaaca ggaactcagc agtctccagc aacttcggtt tcgtctattt
      181 ttacgccagc gtgggacccc ctctgagggc cggcgcctag gcgtctgtgg tctttagagg
      241 ccggggtccc caaaagggga ccccggcctt ttttattctt tcatcatctt ttggggaaag
      301 ggagttggcc agatcggaga gagaacagag acaggcgagg ggattgaata agaccgtcag
      361 ggtgacaaag gtcgcatggc ccgttccttt tcacgggtct gacaaggaac caggcaataa
      421 caaggaggag aggcagatga aggaaagaaa ggcaacccag cagagaggta tccacgacct
      481 tgtaatgaga agtcccggaa tgaggccgag gccggtggac cttgatttcg acttcgaggg
      541 tcttccaatg gcctggaaag aggccaggga ggcccttgaa cgcaagcggt gacagccttt
      601 cactccagga aggccccgaa gagagacccc agatctacgg tgccgattac ggtgttgctg
      661 tatttttcaa agatatccct tcgaccctct tcgagggccg gggggcagat cccgtacctg
      721 atggatgtgg ctgcctccac catggcggcc agcttgtcgc aggcgtcaat aactgtcccg
      781 tcgacagggt cccattcttc gcaattgtag tttgagttca tttccgacag ggtcaggccg
      841 gaaataatat taccagtgac cggatctctg gccctgttgg agaactcgtc gcttgtcagg
      901 tagcgcaact ctttcctcca gccctccggt attagcggga gaagcctggt ctccatggat
      961 tcgttctcgt attccttgat cagctcttcc agtccttcga ccgaccgttt gaccggggag
     1021 attatatccc gggtaaggac ctcgggcagg tcgtggaaga gtccgccgaa gaaggcgttg
     1081 cgaagcctcc tggggcatcc tccgttttca agggagacga aataggagag cgacgccgcc
     1141 atgaaaaggt ggcccagcac agaggttgcg ggtactcttg gaagctgtgc ccaccggacc
     1201 tggaagcgga gctgcccgca gaggtctatg aaggcgtaaa gccccttgtc ggtcatggtg
     1261 cggcgcatga tcatctccct gacgcccgcc aggtcgatgt ggtcctccag ttgaccctcg
     1321 atatctcttc gggtctcttc cacactggag agctcacggt tctggccgta gatcatcctg
     1381 aactcccagt tggtggccag gtagtgcgac gcacggagga tgcgcttctc ccgccccgag
     1441 aaatccggct ccttcaggta tttcctgaag cgttcaaaaa agcctccgcc cagggattcg
     1501 aggtggcggg ccagcttgtc caggacaagc cggttcagcc tttccccgtg cctttccatc
     1561 atcctgtgga atacgggggg ctttatatct gtgagtacca ccctctggaa gaactcgaaa
     1621 agcccgccct cgacaagccg gacccagtca gggtctcctc cctggtcctc ttcgcagcgg
     1681 gctatcaccc atgctatggc catcttgtgg gcctgtttcg ccagttcggt gaactgggca
     1741 gggcgggcat gatcgttcca gcgcgtcata gaagctgccg agaatactgt ctccactaga
     1801 ccaggtgtga tcattctacc gaccttcctt ttgaaaggat gggagtgcca tttgctccat
     1861 ctcctgatac tctatcacta tcgatcgggg aggtctgatc aggcaatgaa gaaaaccaac
     1921 gcagtgaggc tcctggaaag cagaggagtg gcctttgagc tttggaacta cgagtcggac
     1981 cctgatgatg taagcgcaag ggctgttgca gcaaagatag ggctggctcc ggagatgatc
     2041 ttcaagacgc tggtcgcgcg gggcgacaga acaggggtga tgcttgcctg tgtgggcggg
     2101 gactccgagc tggatctgaa ggccctggct gccgcatcgg gcaacaagaa ggtcgagatg
     2161 gtccacatga gggagataca gtccctgacg ggctatataa ggggaggggt ttccccggtg
     2221 ggtacgaaga ggccctaccc tgtctatata gacgagagcg ccttcgcctt cgacaggatc
     2281 tgtgtgagcg gaggggccag aggcgtccag atcttcatcg atcccagggt cctggccgcg
     2341 gaagtggagg ctctccccgc ccctatcgca aagcaggatc tgcctgagag ttagccccgc
     2401 gacagtagtg ctccacatcc cttacgatgt tgtcgatgtg cctcagcatg gccttttctg
     2461 cgtccccggg atccctcccc gctatggcca ggtagatggg catgtggtcc ccgccaaggc
     2521 ggctgccctc ctgctgccgg atgaaggtga agatctgctg ggtatgtacg tcctgacgca
     2581 ccagcttcag tgtggccagc agtaccttgt tcttccctgc cttggcgatc gcttcgtgga
     2641 aaagcctgtc aatgaccgat atgctgggta cctggctgcg gtcgccgtca aggatctccg
     2701 caaaggccct tatctgctcc aggtcctctg tcgaggcttt cagggccgcc aggcgagccg
     2761 tctccctctc tattgccctc ctggcgacaa gtatgtccag gagatcgccg ggggagtgcg
     2821 tcttcagctc ctcaagcagg gccctggcca gggtttcacc ctttccgagg gcctccagcc
     2881 tttcaagatg ttctctgccc tttgccgaaa ggaccctccc tctgttgctc ctcttctcga
     2941 ggtaaccccc cccgtcaagc tcgcccagaa tgcggcccgc cgtggccata ctgaccgggt
     3001 ctccccgttc cgtcattgaa cgggccagcg atccggctcc catcggttct ggagcctggc
     3061 cgatgatgcg tagggctttg gaggttctct ccgataattt gcttccattt ctcatttcat
     3121 gctgcctcct gttgccctag cctccaaggt aggcccgtcg gatctccggg tccatggcca
     3181 ggtccggacc cggaccggtc ttcacgaaga gcccggcctc aaggatgtac cccctgtcgc
     3241 ttatctccag cgcctgggtc gcgttctgct ccaccatcag aaccgtcaca ccctcgttcc
     3301 ttatacggat cacggtgttc attacggctt ccactatcag gggagccaga ccaagggtgg
     3361 gttcgtccag cagcagtatt ccggggtcgc tcataagggc cctggagatg gcaagcatct
     3421 gctgctcccc gccggagagg gtgccggagc gctggttcag cctctctctc agaatcggga
     3481 agagctggaa acacctctcc atttcggcct ggacgctctc ttcgtccttt ctccggtagg
     3541 cgcccattac caggttctct tttacggtca gggccgagaa gagtcgtctc ctctcaggaa
     3601 ccagcgcaag ccccctggcc acgatctcgt gaggtttcga gggtatgggg ttccccttta
     3661 tcaggatcct tccgccggag ggggggacga cacctgcaag ggtccacatg atggtcgatt
     3721 ttcccgcccc gttggagccc acgatggaga caatctcccc ctcgttcacc gagaaggata
     3781 ctccctggac cgcgtggatc gtgccgtagt ggacgttgag atcttcaacg agaagggcgt
     3841 tcatcgcagg tccttcccct ttccgaggt
//
LOCUS       DAOPKQ010000130         3834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_26317_length_3834_cov_5.202964, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000130 DAOPKQ010000000
VERSION     DAOPKQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26317_length_3834_cov_5.202964"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /gene="hypB"
                     /locus_tag="PK146_05265"
     CDS             <1..532
                     /gene="hypB"
                     /locus_tag="PK146_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPE91686.1"
                     /translation="GKTALLESTLTGAPFRSVVLEGDVATSRDAERIARTGTPVVQIN
                     TRGGCHLEAHLVDKALEELELDGIDVVFIENVGNLVCPAEFDLGEDHKVAVCSVPEGP
                     DKPLKYPHLFSQADAVLLTKVDLLEHLDFDRDLFWGDVRDLNSRAQMLEVSALRGEGV
                     PEWIAVVERWIRGKRS"
     gene            529..2505
                     /locus_tag="PK146_05270"
     CDS             529..2505
                     /locus_tag="PK146_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPE91687.1"
                     /translation="MKEKGGESLIASLNESQAEAVRYCSGPLLVLAGAGSGKTRVLAH
                     KFAWLVSEHDLDPRSIMAVTFTNKAAREMQERVARLLDNDLAGLDIGTFHSYGLRFLI
                     RNYKDLERITGRRASTVFDRSDSRSLVKAVMKELNIDQKQYEPGWILECISKAKSEGD
                     PRSLQPVEMDLPAGAVFKEYQKGLTRQGAVDFDDLLVLPLHLLLKDRELLEREKARFG
                     WILVDEYQDVNRPQYLLLKALSSGRNRIMVVGDPDQSIYGWRGADMNMILNFEKDFPD
                     AKVFVLEQNYRSTSNILGGANGVIRNNFRRKPKKLWTSRSGGDKIRILLAGSERDEAA
                     YVAEEVLKLRGLGFRFGDMAVLYRINAMSRLYEEMFLRNDIPYRIVRGTAFYERREVK
                     DAVSFMRLAVNPLDEAALDRIGNIPSRGLGKKSIDKLKEYIRASGEQGPGIWAKLSED
                     GAGLPGKAGKGAIELGVHMGEIIKRSGEIPSVVEYILEGTGYERELIRMDPEGWRERV
                     DNVRELLSVTSSTEGLETMLAEVALVTDMDLVDEETESVNLMSLHAAKGLEFSAVFIV
                     GLEEAIFPHYRCMDDPDSLEEERRLCYVGMTRAEERLYLSGARSRVLFGTVQRNGFSR
                     FLWEIPDEFKETEDMGGEEASYAGGRPYRRRWGW"
     gene            2465..3355
                     /gene="coaE"
                     /locus_tag="PK146_05275"
     CDS             2465..3355
                     /gene="coaE"
                     /locus_tag="PK146_05275"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPE91688.1"
                     /translation="MLVVGLTGDVGAGKSTVSSVWASLGAHVVSADTIVAALWKRNEM
                     VELAAGRWGERILRPGMALDHSAISRIVFGDEKEYRWVCETIHPLVREEMERVVESLD
                     GWIVAEIPLMFENGVPDWIDLTVYIEAPEDERVIRNASRGWDRDELRRRERWLLGSDR
                     KKKMADLVLCNDGTREELEERARELGSRLLSLSSLVRVCFALGSSGASRRLFRELSRN
                     GRVLEVEIAPGEECKWRDVFHVDPGLIVSAIVRPKDLEETRSMAARISGEGGPVCSIL
                     SGERRLPKEVLMRAVGSDKG"
     gene            3352..>3834
                     /locus_tag="PK146_05280"
     CDS             3352..>3834
                     /locus_tag="PK146_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPE91689.1"
                     /translation="MKIITTHIGADFDSLASMVAASKLYPGARACFSGSAGRNVREFL
                     KRTPGRWEILTPSQVDMDKISMLIVVDARSPARIASFAGILGRPGLEVHVYDHHAAVQ
                     DDIVASYSLIEPVGATTTLLVEKLLDASIPVTPQEATLFAMGIYEDTGALTFGATSEK
                     D"
BASE COUNT          857 a          981 c         1261 g          735 t
ORIGIN      
        1 aggcaagacg gcgctgctgg agtcgaccct gacaggagca ccattcaggt cggtagtgct
       61 ggaaggcgat gtggccacat cgagggatgc cgaaaggatc gcccggaccg gaaccccggt
      121 ggtccagatc aacacaaggg gaggctgtca ccttgaggcc cacctggtgg acaaagccct
      181 tgaagagctc gagctggatg ggatcgatgt ggtcttcatc gagaacgttg ggaatctggt
      241 atgcccggcg gagttcgatc ttggagaaga ccacaaggtt gccgtctgca gtgttccaga
      301 aggtcccgat aagcccctca agtatcccca tctcttttcc caggccgacg cggtgctcct
      361 tacgaaggtg gacctcctgg agcacctgga ttttgacagg gaccttttct ggggcgatgt
      421 cagggatctg aactccagag cacagatgct ggaggtctcc gccctcaggg gggagggagt
      481 gccggagtgg atagcagtgg tggaacgttg gatcaggggg aagagatctt gaaggagaaa
      541 ggcggtgaga gcctgatcgc gtccctgaac gaaagccagg ccgaagctgt tcgctactgt
      601 tccgggcccc tgctggtcct ggccggggcg ggaagcggca agacccgcgt tctcgcccac
      661 aagttcgcat ggctggtatc tgagcacgat ctcgaccccc gttcgataat ggccgtcacc
      721 ttcaccaaca aggccgcccg ggagatgcag gagagggtcg cccggctcct ggacaacgac
      781 cttgccggcc tggatatagg aaccttccac tcctacggtc tgcgattcct gataaggaac
      841 tacaaagatc tcgagaggat cacgggacgg agggcgtcaa ccgtctttga caggtcggac
      901 tcaaggtcgc tggtgaaggc tgtgatgaag gagctgaata tcgaccagaa gcagtacgag
      961 cctgggtgga tattggaatg catctcaaaa gcaaagagcg aaggggatcc gcgaagtctt
     1021 cagccggtgg agatggatct ccccgccggc gccgtcttta aagaatacca aaaggggctg
     1081 accaggcagg gcgccgtcga tttcgacgac cttctcgtgc tgccactgca cctgctgcta
     1141 aaggaccggg agctgctgga gagggagaag gccaggttcg gctggatact ggtcgacgaa
     1201 taccaggacg tcaaccgccc ccagtatctt ctcctgaaag ccctgtcatc cggccggaac
     1261 aggatcatgg tcgtcggcga ccctgatcag tccatatacg gctggcgcgg ggccgatatg
     1321 aacatgatcc tgaacttcga gaaggatttt cccgatgcaa aggtctttgt cctggagcag
     1381 aactaccgtt ctacctccaa cattctggga ggcgcgaacg gagtcatcag gaacaacttc
     1441 cgaagaaagc cgaagaagct ctggacctcg agaagcggcg gcgataagat ccggatcctg
     1501 ctggcgggca gcgagaggga cgaggcggct tacgttgccg aggaggtgct gaagctcagg
     1561 gggcttggtt tcaggttcgg cgacatggcc gtactttaca ggataaatgc catgagccgg
     1621 ctctacgagg agatgttcct ccggaacgat attccctaca ggatagtaag gggaacggcc
     1681 ttttacgaga ggagggaggt caaggatgcc gtctccttca tgaggctggc cgtcaacccc
     1741 cttgatgagg cagccctgga caggataggt aacataccct cccggggcct ggggaaaaaa
     1801 tcaattgaca agctcaagga gtatataaga gcttccggcg agcagggacc cgggatctgg
     1861 gcgaagctgt ccgaggacgg ggccggtctg cccggaaagg ccggcaaggg agcaatagag
     1921 ctcggagtcc acatgggcga gatcattaaa cggagcggag agatcccctc cgtcgtagaa
     1981 tacatactgg aaggaaccgg ttatgaaagg gaactgataa ggatggaccc cgaagggtgg
     2041 agggagaggg ttgacaacgt cagggaactc ctgtccgtca cctcttcgac agaggggctc
     2101 gagacgatgc ttgcagaggt cgccctggtt accgacatgg accttgttga tgaagagacg
     2161 gaatcggtga acctgatgag cctccacgct gccaagggac tggagttctc cgcggtattc
     2221 attgtaggtc tggaggaagc catattccct cactaccgat gcatggacga tcctgacagt
     2281 ctcgaggagg agcgccggct ctgctacgtg ggtatgacaa gggccgagga gaggctctat
     2341 ctttcagggg caagaagcag ggttcttttc ggaacggtac agaggaacgg tttctcccga
     2401 ttcctgtggg agatccctga tgaattcaag gagaccgaag atatgggcgg ggaggaagca
     2461 tcctatgctg gtggtaggcc ttaccggaga cgttggggct ggtaaatcaa cggtctcgtc
     2521 agtctgggca tccctggggg cgcatgttgt cagcgccgac acgatcgtgg cggcgctctg
     2581 gaagagaaac gagatggttg aactcgccgc agggagatgg ggtgagagga tacttagacc
     2641 cggaatggct ctggaccatt cggccatctc ccggatagtc ttcggagatg agaaggagta
     2701 ccgatgggtc tgcgagacta tccaccctct tgtgagagag gagatggaga gggttgtcga
     2761 aagcctggac gggtggatcg ttgcagagat ccccctgatg ttcgagaacg gggtgccgga
     2821 ctggatcgat ctcaccgttt acattgaagc tcctgaagat gagagggtca tccgcaacgc
     2881 ttccaggggc tgggacaggg atgagttaag gaggcgcgag cgctggctcc tgggatcgga
     2941 caggaaaaaa aagatggccg accttgtcct ttgtaacgac gggacccggg aggaactgga
     3001 agagagagcc agggagctgg gatcccgctt gctctcgcta tcctccctgg tgcgggtctg
     3061 tttcgcgctg ggttcgtctg gagcctcccg ccgactcttt cgtgaactct cccgaaacgg
     3121 gagggtcctc gaggtggaga tagctcctgg tgaggagtgc aagtggaggg acgtcttcca
     3181 cgtggatcct ggcctgatcg tctccgcgat cgtaagaccc aaagacctgg aggagaccag
     3241 gtccatggct gcaaggatat caggcgaggg aggaccggtc tgctcgatcc tgtccgggga
     3301 gagacgcctt ccgaaagaag tactgatgcg ggccgtgggg agtgacaagg gttgaagatc
     3361 atcaccaccc atatcggtgc cgattttgat tcactggcca gcatggtggc cgcctcgaag
     3421 ttatacccgg gggcccgggc atgtttttcg ggttcagcgg ggagaaacgt cagggagttt
     3481 ctcaagagaa cgcccgggag atgggagatc cttacaccaa gccaggtaga catggacaag
     3541 atctcaatgc tgatcgtggt cgatgcccgt tcccctgccc ggatagcatc ctttgcaggg
     3601 atccttggcc gtccggggct ggaggtgcac gtctacgacc atcatgcggc ggtccaggac
     3661 gacatcgtag cctcctattc gcttatcgag ccagtggggg ccacgaccac actcctggtg
     3721 gagaaactcc ttgatgcgag tatcccggtc acaccgcagg aggccaccct ttttgccatg
     3781 ggcatctacg aggacacggg ggctctgacc ttcggcgcca cttccgagaa ggac
//
LOCUS       DAOPKQ010000131         3833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_26325_length_3833_cov_3.962943, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000131 DAOPKQ010000000
VERSION     DAOPKQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26325_length_3833_cov_3.962943"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK146_05285"
     CDS             complement(<1..599)
                     /locus_tag="PK146_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPE91690.1"
                     /translation="MAGTEISPEIRKQIMLFQRTEITEYNIYQRLARRMEGKNREVLE
                     RISLDEKRHAGVWRRYTGVDPGPDRLMMFFYLMLAFVFGITFAIKIMERNEESAEAAY
                     GAVADLVPEARQIMHEEEEHEKALTDLLDEERLELQERLLAPPRRCPQVCKPFTTAHD
                     LELVHAAALRPKRRDPLRERRCLGHRSDLCVGAHQHLGH"
     gene            752..2008
                     /locus_tag="PK146_05290"
     CDS             752..2008
                     /locus_tag="PK146_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE91691.1"
                     /translation="MYKPFSVDYLNLLLSFSDAVDLASTRLSQHQIRTAFICWEMGKA
                     AGLDSKDTDDLFTAALIHDIGALSPEDKIDLHRVEVYDTEKHCILGKKLLGPVPGFER
                     IAGIVRHHHTPYPKLVPLSRKDALLAQILNLADNIERAIDRTRFILHQSDDISNLVGG
                     LSEAILPEAIDLFLKVSVREDFWLDVVSQRIYGYLLNSGPCRHRVLDLQQVRDFSRLF
                     RNMIDYRSHFTATHSAGVSASAVALAGFMGKSEIGCSLMEIAGDLHDLGKLAIPNSIL
                     MKPDRLTPEEYAIVKQHTYFTYSVLISIEGMQQIAEWAAFHHERLDGDGYPFHLTGKE
                     INLGSRIMAVADILTALAEERPYRAGLPQDKVMGVIRKQVGSGALDGGVVDALEADYR
                     EIIARMLKSQHEAHEYFVKEIQPLTV"
     gene            2031..2456
                     /locus_tag="PK146_05295"
     CDS             2031..2456
                     /locus_tag="PK146_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE91692.1"
                     /translation="MVRILKVVNRDRCIGCLSCMYACTRKLRGLGGTGKSAMRVRAYS
                     GAEGAFSIRVCSRCVRPDCAAACPTGALTTVEGGGVRLDRDKCISCGACVKACTIAAL
                     QWDEEDGVPIPCIFCGQCVDFCPNGVIALVERTPSGEGE"
     gene            2458..>3833
                     /locus_tag="PK146_05300"
     CDS             2458..>3833
                     /locus_tag="PK146_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPE91693.1"
                     /translation="MQGRVLFTDLTSGKSRIEEDGGLFTELLGGTAVATELLARHGNS
                     ETDPLSPLAPVIFTIGPFSALYPVATKTVALFRSPLTGELGESHAGGRLAAALRETGI
                     DALVITGASDRPVFLHIDGQEAKVRSAGTFWGQSAPATDRILREALEKKGRKLSIVRI
                     GPAGERLSPMACATVDSSRHFGRLGLGAVMGSKKLKAIAVSGKGSVVIADSRAYGSVY
                     QTIYDRVVRSGEMKKYHQLGTPANILPLNAIRGLPTRNFTQGFFENASRISGEAFLEE
                     VNVQHIACAHCPCGCIQVAELREKFAPAHYSTVRVSYDYEPIFAVGTQLSISTPREIL
                     RLIAFIEKQGWDCMSMGNTLAWATEAFLSGAIGEAETEGLSLSFGDTRSYLETMQRIS
                     SGNGEFFSDLEKGTAFCASKYGGKDFAMHYGGLEPAGYLTGENAVITWIAGVRHSHLD
                     DNGYSLDQ"
BASE COUNT          821 a         1100 c         1112 g          800 t
ORIGIN      
        1 tgatggccga gatgctggtg agcgcctaca cagagatcgg agcggtgacc aaggcagcgg
       61 cgctctcgga gcgggtcgcg tcgcttcggg cgcagagccg cggcgtggac gagctcgagg
      121 tcatgcgcgg tcgtgaacgg cttgcacacc tgcgggcatc gacggggcgg ggcgaggagg
      181 cgctcttgga gctcgaggcg ctcttcgtcg agaaggtccg tcagtgcttt ctcgtgctcc
      241 tcctcctcgt gcattatctg ccgtgcctcc ggcacaagat ccgccaccgc cccgtaggct
      301 gcctcggccg actcctcgtt cctctccatt atcttgatgg cgaaagtgat cccgaatacg
      361 aaggcaagca taaggtagaa gaacatcatt aaacggtccg gtccggggtc gacacctgta
      421 tagcgtcgcc ataccccggc atgccgcttc tcgtcaaggg agatcctctc gaggacttcc
      481 cggttcttcc cctccattct cctggccagt ctctggtaga tgttgtactc ggttatctcc
      541 gtcctctgga agagcattat ctgctttcgg atctccgggg agatctctgt gcccgccata
      601 agggaatacc cccttcaaaa ctgtctgatc cacggagttg cgcctgtttc ctgtatcttt
      661 gctcaagata ctatatcagg acagttcccg gtacctgagc ccatatctga tatgatactc
      721 cccaagacag aacataatcg aggtactgac catgtataaa ccatttagcg tagactatct
      781 gaacctgctg ctttccttct ccgatgcagt ggatcttgcc agcaccaggc tctcccagca
      841 ccagataaga acggccttca tctgctggga gatgggcaag gccgccgggc tcgactcgaa
      901 ggatactgac gatctcttca ctgccgccct gatccatgac ataggggccc tctcgccgga
      961 ggacaagata gacctccacc gggtggaggt ctatgacacg gagaagcact gcatcctcgg
     1021 gaagaagctc ctgggccccg tcccgggttt tgagaggata gccgggatcg tgcgacatca
     1081 ccatactccc tatccgaaac tggtccctct ctcccggaag gacgcgctcc ttgcccagat
     1141 ccttaacctt gcagacaata tcgagagggc tatcgacaga acccgattta tactgcacca
     1201 atccgacgat atttcaaatc tggtcggggg tctatcggaa gcgatactcc cggaggcgat
     1261 agatcttttc ctgaaggtgt cagtgaggga ggatttctgg ctcgacgtag tatctcaaag
     1321 gatctacggc taccttctca attcgggacc ctgtcgccac cgggtcctgg atcttcagca
     1381 agtgagggat ttttccaggc tgttccggaa catgatagac taccgttcac acttcacagc
     1441 cactcactcg gctggtgtgt ccgcctcggc ggtagccctt gcgggcttca tgggcaagag
     1501 tgagatagga tgcagcctta tggagattgc cggtgatctt cacgaccttg gcaagcttgc
     1561 catccccaac agcatactca tgaaaccaga cagacttacc cccgaagaat acgcaattgt
     1621 taaacagcac acctacttca cctattcggt gctcatatcc atagaaggga tgcaacagat
     1681 agccgagtgg gccgccttcc atcatgaacg tctcgacggg gacggatacc ctttccatct
     1741 cacggggaaa gagatcaacc tggggtcaag gatcatggct gtagcggaca ttcttacagc
     1801 cctggccgag gagaggccct acagggccgg gctcccccag gacaaggtga tgggggtcat
     1861 cagaaaacag gtcggatcag gtgctctcga cgggggagtg gtggatgccc ttgaagctga
     1921 ctaccgtgag atcattgcgc gtatgttgaa gtcacagcac gaagctcacg aatactttgt
     1981 gaaggagatc cagccgttga cggtttgacc gttaaggcga ggaggacatc atggtcagga
     2041 tcctgaaagt agtgaaccgc gacaggtgca taggctgcct tagctgcatg tacgcctgca
     2101 cgcgcaaact gagggggctc gggggtacgg gcaaatccgc catgcgcgtc cgggcctatt
     2161 cgggagccga aggggccttc tcgatccggg tctgctcgag atgcgtccgt ccggactgtg
     2221 ctgctgcctg tcctactggg gcgctgacaa ctgttgaggg cggtggcgtg aggctggaca
     2281 gggataagtg catttcatgc ggggcctgcg ttaaggcatg taccatagcc gcgctccagt
     2341 gggatgagga agacggtgtc cctatcccgt gcattttctg cgggcagtgt gtggacttct
     2401 gtccaaacgg cgttattgcc cttgttgaac gcaccccgtc cggggaaggt gagtagcatg
     2461 caagggaggg tactctttac cgacctcact tctggaaaga gcagaataga ggaagatggc
     2521 ggcctcttta ccgaactcct tggtggaaca gccgttgcta ctgaactgct agcaagacac
     2581 ggtaattcgg aaaccgaccc cctctcgccg ctagccccgg tcatttttac catcggtccc
     2641 ttcagcgccc tttacccggt tgcgacaaag accgtggcac tcttccgctc gcccctgact
     2701 ggcgaactgg gagaatccca tgcggggggg cgattagcgg cggcgcttag agagacgggg
     2761 attgatgccc tggtgataac cggagcctcc gaccggcccg tctttctgca tatcgacgga
     2821 caggaggcga aagtcagatc tgccggaacc ttctgggggc agtcagcccc ggccactgac
     2881 aggatcctcc gcgaagctct cgaaaagaag ggaaggaaac tctcgatcgt caggataggt
     2941 cctgcagggg agaggctatc cccgatggca tgcgcgacgg tggacagttc gcggcacttc
     3001 ggtcgccttg gcctcggcgc cgtaatgggt tccaagaaac tcaaggcaat agccgtcagc
     3061 ggcaagggat ctgtcgtaat agccgactcc agggcttacg ggtctgtcta ccagactatt
     3121 tacgacaggg tggtccggtc aggcgagatg aaaaagtacc atcagctcgg gaccccagcc
     3181 aacatccttc cccttaacgc gatacggggc cttcccacca ggaacttcac ccagggcttt
     3241 ttcgagaacg cttccaggat ctcgggcgag gcctttctcg aggaagtgaa cgtacagcac
     3301 atagcctgcg cccactgccc ctgcggctgc atccaggtgg cagaactgcg ggaaaaattt
     3361 gctcccgccc actacagcac cgtcagggtc tcctacgact acgagccaat cttcgcagtg
     3421 gggacccagc tgagcatctc cacgccgcgg gagatactca ggcttatcgc cttcatcgaa
     3481 aagcaggggt gggactgcat gagcatgggc aacacccttg cctgggccac cgaggcattc
     3541 ctgtcggggg ccatagggga ggccgaaacg gaaggtcttt cgctatcctt tggggatacc
     3601 cggtcatacc tggagaccat gcaaaggata tcttcaggca atggggaatt cttcagtgac
     3661 cttgagaaag gcactgcctt ctgtgcctcg aagtacggcg gcaaggattt cgccatgcat
     3721 tacggcgggc tcgaaccagc agggtacctg acgggtgaga acgcggtaat tacctggatc
     3781 gccggggtca ggcactccca ccttgacgac aacggctaca gcctcgacca gaa
//
LOCUS       DAOPKQ010000132         3823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_26424_length_3823_cov_4.368896, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000132 DAOPKQ010000000
VERSION     DAOPKQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26424_length_3823_cov_4.368896"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PK146_05305"
     CDS             <1..634
                     /locus_tag="PK146_05305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPE91694.1"
                     /translation="TEATDVKTVAGRVAGIVSSRGDIDAPVVVNAAGGYSQFISKMAG
                     VEIPNFSERHEILITEPVEPGVCPPMLMSFSGNYYIQQRPHGSIIGGCSPEGHPQDYE
                     LGNSWDFLEMMSRTFVRLLPRTKGVRVVRQWSGLYNMTPDRQPVLCEADNVKGFFLSC
                     GYSGHGFMFGPISGELLAQLILGQEPWIPIGMLHYSRFERGELLIEPAVV"
     gene            853..1053
                     /locus_tag="PK146_05310"
     CDS             853..1053
                     /locus_tag="PK146_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE91695.1"
                     /translation="MPKKFKVEVNSHWCKGCGLCLAVCPKKVFDFDENLKSTPARMED
                     CIGCKQCENICPDLAITVEGSA"
     gene            1055..2191
                     /locus_tag="PK146_05315"
     CDS             1055..2191
                     /locus_tag="PK146_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPE91696.1"
                     /translation="MSRVEFWQGNVSLAYGAIAAGCRFFGGYPITPSSEIAEVMAEEM
                     PACNGRFIQMEDEIGGIAATIGGAVTGYKSMTATSGPGFSLKQENLGLAYMAEIPIVV
                     VDVMRGGPSTGLPTSIAQQDVMQARWGTHGDHATIAYAPSSVQECYEVAIKAFNMAER
                     FRQPVLIMSDEVLGHMREKVIVPDKETLAIVDRKKPSCSPEEFIPYRPDPEDNLPPMA
                     SFGDGYRWHITGLTHNEWGFPTNCPEEIAKKSARLMDKVNAYRDEIVEYETENVEDAD
                     VIVVAYGSVARTALSAVRHARDKGLKAGYFRPVTLWPFPDRELEPLAEKAGRLIVPEL
                     NFGQMVHEVERAVHGKAAIAQMSLVNGELFQPSEILEKIEEVAG"
     gene            2191..3012
                     /locus_tag="PK146_05320"
     CDS             2191..3012
                     /locus_tag="PK146_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPE91697.1"
                     /translation="MPRPEVLDWLRKRFFPHMWCPGCGHGIIMHAILRALVDSGKKRE
                     ETVIASGIGCSSRMPGYIDSCTIHTTHGRSLAFATGIKLANPELTIVDVMGDGDCTAI
                     GGNHFIHACRRNIGITAVVMNNNIYGMTGGQASPTTPPGARATTAPYGAIDPPFDVCK
                     LAQGAGATYVARATIAQPVLCEQYIKNGIANKGFSVIEIISSCHTQFGRKNNRRTPVD
                     NLNYFKNNSVPLSRAQSMSPVDLEGKIVTGEFVKREAPEYTELYRSLVERVGGGK"
     gene            3012..3566
                     /locus_tag="PK146_05325"
     CDS             3012..3566
                     /locus_tag="PK146_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE91698.1"
                     /translation="MRDRYEIRFAGSGGQGVILAAVVVGEAAAIYEGLNSVQTQAYGP
                     EARGGKSKSEVVISRGSIDYPKATSPDLQIILNQTSCDEFIGQTARGGVVVVDDTYVS
                     SVSESEAEVFMLPIVKTAREKIGRELVTNMVALGVTARVLEKEGLMKADSVKKAILAR
                     VPKGTEEMNEKAFNEGYSLMKAIR"
     gene            3682..>3823
                     /locus_tag="PK146_05330"
     CDS             3682..>3823
                     /locus_tag="PK146_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPE91699.1"
                     /translation="MDELLKHGQVLKVDIETVNSRGQGIARFGGERFVLFVPGALPGE
                     VVR"
BASE COUNT          908 a          995 c         1123 g          797 t
ORIGIN      
        1 cacagaagca accgatgtca agaccgttgc cggccgggtg gccggaattg tttccagcag
       61 gggagatatc gacgctcctg tcgtggtgaa cgctgcaggc ggctactcgc agttcatatc
      121 caaaatggca ggagtggaga tccccaactt ctctgaaaga catgagatcc tgatcaccga
      181 acccgtcgag cccggggtct gccctcccat gctcatgagc ttttcgggga actactacat
      241 tcagcagaga cctcacggat cgataatcgg agggtgcagc cccgaggggc atcctcagga
      301 ttacgaactt gggaattcat gggacttcct tgagatgatg tcccggacct tcgtgcggct
      361 ccttcccagg accaaagggg taagggtcgt ccggcagtgg tcggggctat acaacatgac
      421 cccggaccgt cagccggttc tttgtgaggc tgacaatgtc aagggtttct tcctctcctg
      481 cggctactcc ggacacggtt tcatgttcgg tcccatatcg ggagagcttc tggcgcagct
      541 tatacttggc caggaaccct ggatacccat cggaatgctg cattacagca ggttcgaaag
      601 aggagaactt cttattgagc cggcagtagt ctagtaccat ttatctcccg gtccaggagg
      661 gggcggtttg ccccctcctg gaccatcttg tagaaggata gaattgtctt tacttgacgg
      721 taaattccgt tttgtaatgc aatattcaat atatagatac caatatggct tgtccctgcc
      781 ctcacaaggt ggcataatgg gcccgggtaa tgagatctca tgatatgaac gtttcggagg
      841 aaggtgagtt gtttgccaaa gaaattcaag gttgaagtga attcccactg gtgtaagggc
      901 tgtggactgt gcctggccgt atgccccaag aaagttttcg atttcgacga gaacctgaaa
      961 tccacccctg caaggatgga agactgcatt ggctgcaagc agtgtgagaa catatgcccc
     1021 gacctggcta tcacagttga agggagtgct taggatgtcc agggttgaat tctggcaggg
     1081 gaacgtttct ctcgcctacg gagccattgc cgccggttgc cgttttttcg gggggtatcc
     1141 aataacccct tcttcggaga tagccgaggt aatggccgag gagatgcctg cctgcaacgg
     1201 caggttcata cagatggagg acgagatcgg cggcatagcc gcgacaatag gtggtgctgt
     1261 gaccgggtac aagtccatga cagcaacttc cggtcccggt ttctcgctca aacaggaaaa
     1321 cctggggctc gcctacatgg ccgagatccc catagtcgtt gtagacgtaa tgcgcggagg
     1381 accttcgaca gggctcccca cttccattgc ccagcaggat gtcatgcagg cccgttgggg
     1441 cactcacggc gatcatgcca ccatcgccta tgcaccatca tcggtgcagg agtgttacga
     1501 agtggctata aaggctttca acatggcgga gcgtttcagg cagcctgttc tgatcatgag
     1561 cgatgaggtc ctgggccaca tgcgagagaa ggttatagtt cctgacaaag agacccttgc
     1621 catagtggac agaaagaaac cttcctgcag ccccgaggag ttcatcccct acagaccaga
     1681 ccccgaggac aacctgcccc ccatggcctc cttcggtgac gggtaccggt ggcacattac
     1741 cggacttacc cataacgagt gggggttccc gacaaactgc cccgaagaga tcgcgaagaa
     1801 gtccgcccgg ctgatggaca aggtcaatgc ataccgggac gagatagttg aatacgagac
     1861 ggagaacgtg gaagatgccg atgttatcgt cgtagcatac ggtagtgttg cccggacagc
     1921 cttgagcgcc gtcagacatg ccagggacaa gggcctgaag gcaggttatt tccgtcccgt
     1981 gaccttatgg cccttcccgg acagggaact ggagcctctg gcggagaagg cgggacggct
     2041 tatcgtacct gagctgaact tcggacagat ggtccacgag gtagaaagag ctgtacacgg
     2101 caaagcggca atagcgcaaa tgagccttgt taacggtgag ctttttcaac cctccgagat
     2161 cctggagaaa atcgaggagg tggccggata atgccacgcc ctgaagttct agactggttg
     2221 agaaagaggt ttttccctca catgtggtgc cctggttgcg ggcacgggat catcatgcac
     2281 gcgatccttc gggcactggt cgattccggc aagaagaggg aagagacagt gatagcctca
     2341 ggtataggct gttcaagccg catgccaggg tatattgact cctgcacgat ccatacaacc
     2401 cacgggaggt ccctggcgtt cgctacgggg atcaagctgg ccaaccccga gctgaccatc
     2461 gtcgacgtaa tgggtgacgg agactgtact gctattggag gcaatcactt tatccatgcc
     2521 tgccggagga acatcggcat taccgccgtg gtgatgaaca acaacatcta cggtatgacc
     2581 ggaggacagg cctcacccac gacacctccc ggtgccaggg ccaccacggc tccctacggt
     2641 gccatagatc cccccttcga cgtctgcaag cttgcccagg gtgcaggggc gacctacgtg
     2701 gcaagggcca ctatcgccca gcccgtcctt tgcgagcagt acatcaagaa cggaatagcc
     2761 aacaagggtt tttccgtaat cgagatcatt tccagctgcc acacccagtt cggcaggaag
     2821 aacaaccgga ggaccccggt ggacaacctt aactacttca agaacaactc tgttcccctt
     2881 tccagggccc agagtatgtc cccggtcgac cttgaaggca agatcgtgac cggcgaattc
     2941 gtgaagaggg aagccccgga gtatacagaa ctctaccgca gcctggtcga gagggttgga
     3001 ggtgggaaat aatgagggat cgctacgaga tacgtttcgc aggttccggc gggcaggggg
     3061 tcattctggc cgctgtcgtg gtaggagagg ccgcggctat ctacgagggg ctgaactctg
     3121 tccagacaca ggcttatggt cccgaggcta ggggtggaaa atccaaatcg gaagtggtca
     3181 tctcaagggg cagtatcgat taccctaaag ccacatcccc ggatctccag ataatcctca
     3241 accagacatc ctgcgatgag ttcataggac agacggcaag gggcggagtg gtcgtagtcg
     3301 atgataccta cgtctcctca gtctccgaaa gcgaagccga ggttttcatg cttcccatcg
     3361 tgaagaccgc aagggagaag atcggaaggg aactagtcac caacatggtg gcccttgggg
     3421 ttactgccag ggttctggaa aaggaaggcc tgatgaaggc cgattccgtc aagaaggcca
     3481 tactggccag ggtcccaaag gggaccgagg agatgaacga aaaggccttc aatgagggct
     3541 acagcctaat gaaagccatc aggtagtctc tggaaatctt ttccagggaa cctttaaagg
     3601 ggcggggggg tgacccccgc ccctttcgtt gtcaaggggc cttgctgtta tagaatatcc
     3661 ataaaacaga aagatgccgg ggtggatgaa ttgttgaagc acggacaggt cctgaaagta
     3721 gatattgaga ctgtgaacag ccgtggccag ggcatagcac gcttcggcgg tgaaagattc
     3781 gtcctttttg tccctggggc acttcctgga gaggtggtca ggg
//
LOCUS       DAOPKQ010000133         3798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_26667_length_3798_cov_4.397275, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000133 DAOPKQ010000000
VERSION     DAOPKQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26667_length_3798_cov_4.397275"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK146_05335"
     CDS             complement(<1..367)
                     /locus_tag="PK146_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE91700.1"
                     /translation="MREEMPRVRWEKIERIILEPCAAFSDASRGRSREEPLCPLRTIF
                     QQDRDRIIHSKAFRRLKHKTQVLLLPEGDHYRTRLTHTLEVCQIARTISRALGLNEDL
                     TDAIALGHDLGHTPFGHMGE"
     gene            complement(454..3123)
                     /gene="ppdK"
                     /locus_tag="PK146_05340"
     CDS             complement(454..3123)
                     /gene="ppdK"
                     /locus_tag="PK146_05340"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPE91701.1"
                     /translation="MTEAGNERKRFVFHFTEGSAEKRSLLGGKGANLAEMLRIGLPVP
                     PGFTISTEACLEFLKRGEQFLEEVWGEIRTAMTDLEKETGKNFGGKTNPLLVSVRSGA
                     AVSMPGMMDTILNLGLNADSVKALGTESGDERFALDSYRRFIQMFSNVVLGMDGSVFE
                     NVLHQVKKDLKVSYDFEIPASSWKGVISRYLKIVEQETGEPFPDDPWVQLEKSVEAVF
                     LSWKNPRAITYRRINKIPDDLGTAVNVMTMVFGNLGEDCGTGVCFTRNPSTGEKKLYG
                     EFLVNAQGEDVVAGIRTPHPIDEFGSKLPEADRELHRIADLLEKHYRDVQDIEFTVER
                     GKLYMLQTRNAKRTARAAVKSTVDMYNEGSIDAATAVSRVTPDQVEQLLHKQIDPDAS
                     FDIAAKGLPASPGAAVGIVIFDADEAEKVAQSGQPVILVRPETTPDDIHGLYAAQGVL
                     TSRGGMTSHAAVVARGLGKPCVSGCESVVIDVARGVMIVGDKTLKKGDILTIDGSKGH
                     VIFGEVPLVQPSFTDDFRELLDIADSVSTLEVWANADTPEDAKRARSFGAKGIGLCRT
                     EHMFMATDRLPVVQEMILARDRQSRVKALDRLKVMQKEDFRGIFRVMDGLPVTIRLLD
                     PPLHEFLPKEHELRDTIASIKTDSEASPEELVHAETVLARVLSLQENNPMLGFRGCRL
                     GLVYPEIYEMQIAAIIEAACELKKEGIDVKPDIMMPLVGTREEMKRLLAMVKDITGSI
                     ITQQGLDDLQYLVGTMIEVPRAALVAGEIAEFAEFFSFGTNDLTQTTFGYSRDDAEGK
                     FLAQYVNDGVLPDNPFHVLDREGVGRLMTLAVAEGRQKYPELQIGICGEHGGNPQSIV
                     FCHELGLNYVSCSPFRVPVARLSAAHSKLGLLD"
     gene            complement(3156..>3798)
                     /locus_tag="PK146_05345"
     CDS             complement(3156..>3798)
                     /locus_tag="PK146_05345"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HPE91702.1"
                     /translation="ETERAMVVCTLVDHDLRRWFMEKARNQGTDVIDILGPILDMLER
                     RLEHPPLETPGLMRRMDEEYFRRVKAIEFAIKCDDGRCAEHLLSADVVILGVSRTSKT
                     PLCMYLAHRGFMVGNVPLIPETEPPGEIFEIPPEKIFGLTVQPEKLVDIRRERLRLLG
                     LDSEISNYAQWERVEEELQFAKNIMRKIGCRILNVTNRAIEETAQEILDYLRA"
BASE COUNT          734 a         1146 c          999 g          919 t
ORIGIN      
        1 tctcccccat atgaccgaaa ggcgtgtggc caaggtcgtg cccgagagcg attgcgtcgg
       61 taaggtcctc gttcagccct aaagcacggg agatggtcct tgctatctgg cacacctcaa
      121 gggtgtgggt aagccgcgtc ctgtagtgat caccttcggg tagcaggagg acctgtgtct
      181 tgtgcttgag ccttctgaag gccttggagt ggataatcct gtcacggtcc tgctggaaga
      241 tggtcctcaa ggggcagaga ggctcttccc tcgatcttcc ccggcttgcg tcgctgaaag
      301 ctgcgcaagg ctcaaggatg atcctctcga ttttttccca gcggaccctg ggcatctctt
      361 ctctcatggc ttctcctttg tatatacagg ggccgccact tgccgtggcg gcccctggtc
      421 tcaaaaacct tgcatccgcc cccgttcagg gtttcagtcc agcagaccca gtttagaatg
      481 tgccgccgaa agccttgcga cagggaccct gaagggggaa cagcttacgt agttcagtcc
      541 cagttcatgg cagaagacaa tactctgggg gttcccaccg tgttccccgc agatccctat
      601 ctgcagttcg gggtatttct gccttccttc ggccacggca agtgtcatca gtcttcctac
      661 cccttcacgg tcaagcacgt ggaagggatt atcggggaga acaccgtcgt taacgtactg
      721 ggccaggaac ttgccctcgg cgtcatccct ggagtatccg aaggtggtct gggtcagatc
      781 gttggtcccg aagctgaaaa actcggcaaa ttcggctatt tcaccggcga caagtgccgc
      841 cctcggcact tcgatcatgg ttccgacaag gtattgcaga tcatcaagtc cctgctgggt
      901 gatgatactg ccggttatgt ccttgaccat cgccagcagc ctcttcatct cctcccgggt
      961 acccaccagg ggcatcatta tatcgggctt gacatcaatg ccttctttct taagttcaca
     1021 ggcagcctcg ataatggcag cgatctgcat ctcgtagatc tcgggataga caagccccag
     1081 ccggcagccc ctgaatccca gcatggggtt gttctcctgg agtgacagga cccttgccag
     1141 cacagtttcg gcgtgcacaa gttcctccgg cgaagcctcg ctgtcggtct ttatggaagc
     1201 aatggtatcc ctgagctcgt gctccttggg taggaactcg tgaagaggag ggtccaggag
     1261 ccgaatggtc acggggaggc cgtccatgac cctgaatatt ccccggaagt cctccttctg
     1321 cattaccttg agccggtcaa gggccttcac ccgggattgc ctgtctctgg ccaggatcat
     1381 ctcctgcacg accgggagcc tgtcggtcgc catgaacatg tgttccgtcc tgcagagacc
     1441 tatgcctttt gccccgaaag acctcgctct tttggcatct tctggtgtat cggcgttcgc
     1501 ccagacctca agggtcgata ccgaatcggc gatatccagc agttccctga aatcatcggt
     1561 gaacgagggc tgtaccagag gcacctcccc gaagatgaca tgccccttcg atccgtctat
     1621 cgtcaggata tcaccctttt tcagggtctt gtcgccgacg atcatgaccc cccttgcgac
     1681 gtcgataacc accgattcac aacccgaaac acagggcttt cctagacctc tggcgacaac
     1741 ggcggcatgg ctcgtcattc cccccctgct ggtcaggaca ccctgcgccg catagagtcc
     1801 atggatgtcg tcgggtgtag tctccggtct taccaggatg accggctgtc ccgattgagc
     1861 gaccttctcg gcctcgtcgg catcgaaaat gacgatcccg acagccgctc ccggagaggc
     1921 cgggagaccc tttgctgcta tatcgaaaga ggcatcgggg tcgatctgct tgtgcagaag
     1981 ctgttcaacc tgatcgggtg ttacccttga taccgccgta gcagcatcga tagacccttc
     2041 attgtacatg tccactgttg actttaccgc ggctctggcg gtccgcttgg catttctggt
     2101 ctgtagcatg tagagtttgc cccgttccac tgtgaactcg atgtcctgga catcccggta
     2161 gtgtttttcc agcagatcag cgatcctgtg aagctcccgg tccgcttcag ggagcttgga
     2221 cccgaactcg tcaataggat gcggtgtcct gatgccggcc accacatcct cgccctgggc
     2281 gttcacaagg aactccccgt aaagcttctt ctcaccggtg gaggggttcc ttgtgaaaca
     2341 gacccccgtc ccgcagtctt ctccaaggtt cccgaagacc atggtcatta cattgacagc
     2401 tgtacccagg tcatcgggga tcttgttgat cctcctgtag gttattgccc tggggttctt
     2461 ccagctgagg aagaccgcct caacggactt ttccagctgg acccaggggt catcggggaa
     2521 gggttctccc gtttcctgct caactatctt gaggtacctg gagattactc ccttccagga
     2581 agaagcgggg atctcgaagt cataggagac cttgaggtct ttcttgacct ggtgcagcac
     2641 gttctcgaag acggacccat ccatgcccag caccacgttg gagaacatct ggatgaatct
     2701 cctgtagctg tccagggcga acctctcatc gcccgactcc gttccgagag ccttgacgga
     2761 atcggcgttg aggcccaggt taagtattgt gtccatcatg ccaggcattg ataccgctgc
     2821 cccggaacgc acggagacca gaagcgggtt ggtctttccg ccgaaattct ttcccgtctc
     2881 cttttccagg tcagtcatgg ctgtccggat ctcgccccat acctcttcaa ggaactgttc
     2941 ccctcttttc aggaattcaa ggcaggcctc tgtagaaatg gtgaaccccg gcggaaccgg
     3001 tagacctatc ctgagcatct cggccaggtt ggcaccctta ccacccagga gggacctctt
     3061 ttccgcactt ccctctgtga aatggaaaac gaacctcttc ctttcatttc cggcttccgt
     3121 cattctttcc gacctccccg gcgatcaatg taggatcaag ctctcagata atccaggatc
     3181 tcctgggccg tctcctcaat ggccctgttg gtgacgttca atatacggca gccaattttg
     3241 cgcatgatgt tcttggcgaa ctggagttct tcctcgaccc tctcccactg ggcgtagttg
     3301 gatatctccg aatcaagtcc cagtagccga agtctctctc gcctaatatc cacaagcttt
     3361 tcgggttgaa cggtcagccc gaatatcttt tcgggcggta tctcgaagat ctcccccgga
     3421 ggttccgttt ctggtataag ggggacgttc cccaccatga aaccccggtg ggcaaggtac
     3481 atgcagagcg gggtcttgct cgtccgggac accccaagga tgaccacgtc cgctgaaaga
     3541 aggtgttccg cacaccttcc atcatcacac ttgatggcga actcaattgc cttcactctg
     3601 cggaaatact cctcgtccat ccttctcatc agacccgggg tctcaagggg ggggtgttcg
     3661 agccgtctct cgagcatatc caggatcggg ccgaggatgt ctatgacgtc ggtcccctgg
     3721 ttgcgggctt tctccatgaa ccatcgcctg agatcgtggt cgacaagggt acaaacgacc
     3781 attgctcttt ccgtctcg
//
LOCUS       DAOPKQ010000134         3777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_26870_length_3777_cov_3.945997, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000134 DAOPKQ010000000
VERSION     DAOPKQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26870_length_3777_cov_3.945997"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PK146_05350"
     CDS             <1..1170
                     /locus_tag="PK146_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPE91703.1"
                     /translation="LQDTPFYGEGGGQVGDRGIVRGEEFLAEVTDTFRAAGDLVVHKV
                     IISEGEAAEGRPVTAQVDGDRRRAIKAHHSATHLLHEALVRVLGRHVTQAGSLVNEEL
                     LRFDFTHMKPLTPEETGEVEMIVNRQIVLNTPLEVIQTDVESARRMGAKALFNEKYGE
                     SVRVVRVPGFSAELCGGTHVDATGDIGSFKIIREEGIGSGIRRITALAGIPSFLHHRK
                     LFERVEHLGRVLSVPSEELEKRTEEIVQENRSLLVRMDRLKSRSALSRVEQLVAGREE
                     TGGVFVVSGAFEGTGPEALREVGDRVRQKVSPVLVVLASEEGGRGSLVAMADREAVEL
                     GVNCGLLVRRIASELGGGGGGRPDMAQAGFSDVAGIPVSLKNVRRIVLEMTGEKA"
     gene            1167..1592
                     /gene="ruvX"
                     /locus_tag="PK146_05355"
     CDS             1167..1592
                     /gene="ruvX"
                     /locus_tag="PK146_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPE91704.1"
                     /translation="MKRILGLDIGRVRIGVAISDPLGTFAQGIDVLEAGKDWMEQVRG
                     LVQERNIGMIVVGVPVRTDGSKGPEAAWVEAVMEKLRKKEGYGGVEIVAWDERFSTAE
                     AERTLIKGDLSRRRRKGVIDKVAATLILQSYLDNRRISS"
     gene            1589..>3777
                     /gene="mutS"
                     /locus_tag="PK146_05360"
     CDS             1589..>3777
                     /gene="mutS"
                     /locus_tag="PK146_05360"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPE91705.1"
                     /translation="MKLPEDVKMTPMLKQYIEWKRRYPECLLFFRMGDFYEMFFEDAR
                     IAASILDIALTARDPEKKIPMAGVPYHSVENYLERLVASGRKVAICEQVSEPDGRTLV
                     DRQVVRVVTPGTFLPAESASEGRLACVNVLPDGVSMALLNFATGVLLAGTLSPSSARA
                     ELAAFSPSEIICPSGGESEAAALVQSVDAPVITSRGKDDFSVPGAARRICREWKVSSL
                     DGFGLSDLDPACGCAAVLLSFLQETQFRSARGAVEIRPLLDKVSMYIDPAAIENLEIA
                     GGSGGSLFDTLNRCSTAMGRRKLKEWLLHPLIDPESISERHEKVETMLQAPEARTRLR
                     RALSACGDVDRALSRISFGSAGPRDLGIIRDTLEAVPAITGQLDRIGLSDLLTLPERL
                     DELRLHLSSALEEELPRTVQGGSVIRRGFDQVIDTLRDVRKNEKEWLESYLEEMKERT
                     GIRNMKIGFNRVFGYFIEISRSSLKDVPPFFTRKQTLVSAERFVTEELGLFEKRMLSA
                     TEEAREREEQLFRELCLSVKDSSPGLRALSNSVAAVDVLVSFAWVAAQEGYCRPNVDY
                     EDRFHVRGGRHPVVENSLVTGGAFTPNDVLLDGGENRIAIVTGPNMAGKSTYLRMAAL
                     LAIMAQAGSFIPAESASLGVVERIFSRIGARDEISRGRSTFMVEMIETANILNNVNSR
                     SLVILDEVGRGTSTYDGMSIAWAILEYLQEQPDRRPKVLFATHFHELT"
BASE COUNT          799 a         1012 c         1251 g          715 t
ORIGIN      
        1 cttcaggata cccccttcta cggagagggt ggcggccagg tcggcgacag gggcatagtc
       61 aggggtgagg aattcctcgc cgaggtaacg gacaccttca gggctgccgg cgacctggtg
      121 gtgcacaaag tgatcattag cgaaggagaa gctgcagaag gccgccccgt gacagcccag
      181 gtcgatgggg accggcgtcg ggccatcaag gctcaccact ctgccaccca ccttcttcat
      241 gaagccctcg taagggtact tggcagacat gtcacccagg ccggttccct tgtcaacgag
      301 gagctcctcc ggttcgattt cacccacatg aagcccctca caccggaaga gacaggggag
      361 gtcgagatga tcgtgaaccg ccagatcgtg ctcaacaccc ccctggaggt gatccagacc
      421 gatgtcgaga gcgccagaag gatgggggcc aaggccctct tcaacgaaaa gtacggtgaa
      481 agcgtcaggg ttgtacgcgt cccgggtttc agcgcggagc tttgcggcgg gacccatgtt
      541 gacgctaccg gggacatagg gtccttcaag atcattcggg aagagggcat agggtcgggg
      601 atcaggagga tcacggcact ggccggcata ccctccttcc tgcaccaccg gaagctcttt
      661 gaaagggtcg agcacctggg gagggtcctt tccgtccctt ccgaggagct cgaaaaaagg
      721 acagaggaga tagtccagga gaaccgttcc ctcctggtgc ggatggatag acttaaaagc
      781 cgctccgccc tttcaagggt agaacagctg gtggcgggtc gggaggagac cggaggtgtt
      841 tttgtcgttt cgggagcttt cgaagggacg gggccggagg ctctcaggga ggtgggcgac
      901 agggtcaggc agaaggtctc ccccgttctg gtcgtgctgg ccagcgaaga ggggggtcgt
      961 gggagcctgg tcgccatggc cgacagagag gccgtggagc tgggggtcaa ctgcggtctt
     1021 ctggtcagga gaatagcctc ggagctggga ggcggcggag gcggaagacc cgatatggcc
     1081 caggcaggtt tcagcgatgt ggccgggatc cccgtctcac tgaagaatgt ccgcagaata
     1141 gttctggaaa tgacagggga aaaggcttga agaggatcct gggtctggac atcggaaggg
     1201 tcaggatagg agttgccata agcgatcccc tgggaacgtt cgcccagggg atcgatgtac
     1261 ttgaagccgg gaaggactgg atggagcagg tcagaggttt ggtccaggag cggaacatcg
     1321 gcatgatcgt cgtcggcgtc cccgtaagga cagacggttc aaagggtccc gaggcggcct
     1381 gggtagaggc tgtaatggaa aagctacgga agaaagaagg ttacgggggg gtcgagatag
     1441 tggcctggga tgagaggttc tccaccgccg aagctgagag gactctcatc aagggggacc
     1501 tctccaggcg gaggagaaag ggcgtaatag acaaagtggc cgcgaccctt atcctgcagt
     1561 cctacctgga taacaggagg atctcctctt gaagctgcct gaagacgtca agatgacccc
     1621 catgctcaag cagtacatcg agtggaagag aaggtatccc gaatgcctgc tcttcttcag
     1681 gatgggcgac ttttacgaga tgttcttcga ggacgcgagg atcgcagcgt caatccttga
     1741 catagccctc acagccaggg atcccgagaa gaagatacct atggcgggcg ttccctacca
     1801 ctctgtcgag aactacctgg agaggctggt ggcctcgggg cggaaggtgg ccatctgcga
     1861 acaggtatcc gaaccggacg gaaggaccct ggtcgacagg caggtggtaa gggtggtcac
     1921 acccgggacc ttcctgcccg ccgaatcggc ttcggagggg aggctggcct gtgtcaacgt
     1981 ccttcccgat ggcgtgtcca tggcactttt gaactttgcc acgggggtgc ttcttgcggg
     2041 gacgctgagc ccctcatcgg cgagggccga gttggcggct ttttcacctt cggagatcat
     2101 ttgcccctcg ggaggagaga gcgaggccgc cgccctggtc cagtcggtcg atgctcctgt
     2161 gatcacctcc aggggcaagg acgacttcag tgtacccggg gccgcgagga ggatctgccg
     2221 cgaatggaag gtctcctctc tcgatggttt cgggctttcc gatctcgatc ccgcctgcgg
     2281 atgtgcagcg gtgctgctat ccttcctcca ggagacccag ttcaggtccg cccgtggtgc
     2341 cgtggagata aggcccctcc tcgacaaggt ctcaatgtat atcgatcctg cggccataga
     2401 gaaccttgag atcgccggag gttcaggggg gtcacttttc gatactctga accgctgctc
     2461 caccgccatg gggcgtcgaa agctgaagga gtggctgctg catccgctca tcgacccgga
     2521 gtccatatcg gaaagacatg aaaaggtcga gaccatgctg caggctcccg aggcaaggac
     2581 aaggctgcgg cgggctcttt cagcctgtgg agacgttgac agggcgctct ccaggatatc
     2641 cttcgggagc gccggaccaa gagatctggg catcataagg gacactctgg aggctgtacc
     2701 ggccataaca ggacaacttg acaggatcgg tctgtcagac ctgctgaccc tcccggagag
     2761 actggatgaa ttgaggctgc acctttcctc tgccctcgag gaagagctcc ccaggactgt
     2821 tcagggcggc agtgtgatcc gtagggggtt cgaccaggtc attgacaccc tcagggacgt
     2881 caggaagaac gagaaggagt ggcttgaaag ttacctggag gagatgaagg agagaacggg
     2941 gatcaggaac atgaagatcg ggttcaacag ggtcttcggt tatttcattg agatcagcag
     3001 gtcctccctg aaggacgttc cgcccttctt caccaggaag cagaccctgg tctcggccga
     3061 gaggttcgtg acggaggagc tggggctctt cgagaagagg atgctttctg ccacggaaga
     3121 ggccagggag cgggaggagc agctcttcag ggagctatgc ctctcggtga aggattcctc
     3181 ccctggtctc agggcgctgt cgaattctgt cgccgccgtg gatgtcctgg tgtccttcgc
     3241 atgggtcgcc gcccaggagg gctattgcag acccaatgtg gactacgagg accgcttcca
     3301 tgtccggggg ggtagacacc ccgtggtgga gaattcgctt gttaccggag gagccttcac
     3361 tcccaacgat gtccttctcg acggtggaga gaaccgtatt gccatagtta ctggtccgaa
     3421 catggccggc aagtcgacct acctgaggat ggctgcactc ctcgcgataa tggcccaggc
     3481 ggggtcgttc atccccgccg agagtgccag cctgggggtc gtagagagga tcttctcacg
     3541 cattggggcc agggacgaga tctccagggg ccggagcacc ttcatggtcg aaatgatcga
     3601 gaccgccaac attctcaaca acgtcaactc caggagcctg gtgatacttg acgaggtcgg
     3661 gagggggact tcgacctatg acggcatgag catcgcatgg gcgatcctcg aatatctcca
     3721 ggaacagcct gaccggcgac ccaaggtact tttcgcgacc cacttccacg aacttac
//
LOCUS       DAOPKQ010000135         3744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_27202_length_3744_cov_4.335050, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000135 DAOPKQ010000000
VERSION     DAOPKQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27202_length_3744_cov_4.335050"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PK146_05365"
     CDS             <1..428
                     /locus_tag="PK146_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HPE91706.1"
                     /translation="PEGNGPAKAMLQAVNKAGWELDEVDLINAHGTSTSLNDTMESSA
                     INRAFGEHAGKTLVHSTKSMIGHTLGAAGALESIAAIQAIVEGVVHPTANCVNPDPEC
                     PINVVPDGKPIQREVSRVLVNNFGFGGHNGVAAIEKYRG"
     gene            409..1149
                     /gene="rnc"
                     /locus_tag="PK146_05370"
     CDS             409..1149
                     /gene="rnc"
                     /locus_tag="PK146_05370"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPE91707.1"
                     /translation="MRSTEVKVGRSSRTRSRTEDNHSPEVLEKLLAYRFRDGQLLLEA
                     LTHASFSNENGLGYCNERLEFLGDAVLELCVSEILYKRFPEATEGELSARRASIVCED
                     TLSAWAIKVGIPSFLRLGRGLCMSGGREQPVLSADAAEAVFGAVFIDGGFEAARSVIE
                     QFLLNFVELEAPVPRNPKADLQELMEKRGLGKPDYEVTEQVGPPHAPVFRVKVTSLGE
                     ILGTGNGRTIREAERKAAEKALEQLCRK"
     gene            1316..1864
                     /gene="pyrR"
                     /locus_tag="PK146_05375"
     CDS             1316..1864
                     /gene="pyrR"
                     /locus_tag="PK146_05375"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPE91708.1"
                     /translation="MLKEKAKVMNEQDMERALKRMSHEILERNRGLRDMILVGIQRRG
                     VYLVNRLRDIFVGLESLKVPTGVLDITLYRDDLTLLHDQPVVHSTAIPEDVNARRILL
                     VDDVLYTGRTIRAALDALMDLGRPSWVQLAVLIDRGHRELPIHPDYVGRSVPTSRSEV
                     VEVRVKELDGTDEVVICEREGF"
     gene            1868..2797
                     /locus_tag="PK146_05380"
     CDS             1868..2797
                     /locus_tag="PK146_05380"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPE91709.1"
                     /translation="MEWRHRHVLDLDSWDRSELQHILEQTRSMDELLDRPIKKVPALR
                     GKLVVNLFFEPSTRTRVSFELAEKFLSADVVNWTASGSSTGKGETLRDTAWTLEAMGA
                     DAVVVRHGAVGAADYLARKMRKSVVFNGGDGAHAHPTQALLDLYTAWKRLGDLNGRKL
                     AMIGDVLHSRVARSDIIAFSRMGVKVELSGPPTLMPRAPEAMGAIFRKDPHDAVRDAD
                     IIYLLRIQRERQEEGMFPSLDEYHQFFGADSRLMESASPDALVMHPGPINRRVEISSE
                     VADGPQSMILGQVRSGVALRMALLFVCLGGVRQ"
     gene            2794..>3744
                     /locus_tag="PK146_05385"
     CDS             2794..>3744
                     /locus_tag="PK146_05385"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPE91710.1"
                     /translation="MKILLRNAILFDGVSLSGKETDVSIDGSLIEAIGDADLGRDFDV
                     IHDLSGKILCPGFIDLHCHLRDPGQEWREDLSSGSSAGAAGGFTTLVCMPNTDPPLDS
                     PALVSYVLEKSGRSPGSRILPAGCVSRERKGKVLAEMGKMAKAGAVIFTDDGNPVSDT
                     GLLRLALQYSSWLGVRLMEHPEEKSLTKDAQVNEGICSSFSGLKGWPSSAEASDISRG
                     VSLCRDTGVPIHFTHVSAEDSLRVLREAKASKLPVTFDVTPHHLCLSEENVIEGGYDS
                     VFKVNPPLRSRRDRDALWEALAEGTVDAIATDHAPWHIDEK"
BASE COUNT          871 a          957 c         1146 g          770 t
ORIGIN      
        1 acccggaagg gaacggtccc gcaaaagcaa tgcttcaggc cgttaacaag gccgggtggg
       61 aacttgatga ggtggacctt atcaacgccc acggtacatc gaccagcctg aacgatacca
      121 tggagtcctc ggcgatcaac agggctttcg gagagcatgc cggaaagacc ctcgtccatt
      181 ccaccaaatc aatgatcgga catacccttg gagctgcagg ggcgctggag tccatcgccg
      241 ctattcaggc catagtagag ggggttgttc accccactgc caactgtgtc aatccagatc
      301 cggaatgtcc cataaacgtt gtaccggacg ggaaacctat ccaaagggag gtctccaggg
      361 tcctcgtcaa caactttggt tttggaggcc ataacggggt tgcagcgatt gagaagtaca
      421 gaggttaaag tgggcagatc ctccaggacc aggtcgagga ccgaagacaa ccatagcccg
      481 gaggtgcttg aaaagcttct ggcttataga ttcagggacg gacagttgct gctggaagct
      541 ctgacccacg catccttttc caacgagaac ggcctcggtt actgcaacga gagactggaa
      601 ttcctgggag atgccgttct agagctctgt gtgtcggaga tactctacaa aagattcccg
      661 gaagcgaccg aaggggaact atctgcgaga agagcatcga tcgtttgtga ggataccctg
      721 agcgcctggg cgatcaaggt agggataccc tccttcctga gattgggcag ggggctttgt
      781 atgagcggcg gtcgcgaaca gcccgtatta agcgctgacg cggctgaagc agtgttcgga
      841 gcagtgttta tcgatggagg gtttgaagcg gcccgaagtg tgatagagca gttcctgctg
      901 aatttcgtag aacttgaggc cccggttcca aggaatccca aagccgatct tcaggagctc
      961 atggagaaaa ggggacttgg aaagcctgac tacgaagtta ctgagcaggt cggtcctcct
     1021 cacgcacctg tcttcagggt taaagtgacg agcctgggag agatccttgg caccgggaac
     1081 gggaggacca tcagggaggc tgaaaggaag gctgctgaaa aggccctgga acaactctgc
     1141 agaaaataat gcaaccggtt gcattattcc ccctctctga taaaatcctc ttgaggggat
     1201 gggtgcctga aaatgagaga tatggtcctt aaaggcgagc ttaacaggaa atttaaagag
     1261 ggttgcgggt ccccaacggg gtctgcgccc ttttttgtat gaaggaggtg gtctcatgct
     1321 gaaagagaaa gcgaaagtga tgaatgaaca ggacatggag agggccctca agaggatgtc
     1381 ccacgagatc cttgaaagga acaggggcct gagggacatg atcctggtgg gtatccagag
     1441 aaggggagtc taccttgtca accgactgag ggatatcttc gttggactgg aatccctgaa
     1501 ggttccgaca ggggtcctgg atatcacact ctacagggac gacctgaccc tcctgcatga
     1561 ccagccggtg gtccacagca cggccatacc agaggacgtg aacgcccgaa ggatacttct
     1621 cgtggacgat gtgctctaca cgggacgcac gatcagggcc gccctggatg cattgatgga
     1681 tcttgggcga ccctcctggg tgcagcttgc ggtccttata gacagggggc atcgggaact
     1741 gcccatccat cccgattatg tgggtcggag cgtgccaaca tcaaggagtg aggtcgtgga
     1801 ggtccgggta aaggagctgg acggaaccga cgaggtcgtt atatgcgaga gggaggggtt
     1861 ctgacgaatg gagtggagac atcgtcacgt tcttgacctt gactcctggg acaggtcaga
     1921 gctccagcac atccttgaac agacccggtc aatggacgaa cttcttgaca ggcctatcaa
     1981 gaaagtccct gccctcaggg ggaaactggt ggttaacctt ttcttcgagc cgtcaacaag
     2041 gaccagggtc tcctttgagt tggcggaaaa attcctaagc gccgatgtgg tcaactggac
     2101 tgcctccgga tcgagcactg gcaaggggga gaccctcagg gatactgcct ggaccctgga
     2161 agccatggga gccgacgcgg tggtagtaag gcacggtgca gtgggcgctg cggattacct
     2221 tgcgagaaaa atgaggaaga gcgttgtctt taacggtggt gacggtgcac acgcgcatcc
     2281 gacacaggcc cttctcgatc tttacacggc atggaagagg ctgggagatc tcaatggccg
     2341 caaactggcc atgatcggtg atgtcctcca tagccgtgtg gccaggagcg atattatcgc
     2401 tttcagcaga atgggggtaa aggtcgaact atcaggtccc ccgaccctga tgccccgtgc
     2461 accagaggcc atgggggcca ttttccgcaa ggacccgcat gatgccgtaa gggatgccga
     2521 cattatctat ctgctgagga tacagcgtga gaggcaggaa gagggaatgt tcccctctct
     2581 ggatgaatat caccagttct ttggtgccga cagcaggctc atggagtcgg ccagcccaga
     2641 cgccctggtg atgcatccgg gtcccataaa ccggagagtg gagatctcaa gcgaggtggc
     2701 agacgggccg caaagcatga tcctgggcca ggtacggagc ggcgttgcac taaggatggc
     2761 ccttctcttt gtatgtcttg gaggtgtaag acagtgaaga tccttctccg caacgccatt
     2821 cttttcgacg gggtcagtct ttccgggaaa gaaacggacg tctctatcga cggcagcctt
     2881 atcgaagcca ttggagacgc tgacctcggg cgggattttg acgtcattca cgacctttcc
     2941 gggaagatcc tctgccctgg ttttatcgac cttcactgtc atctcaggga tcccggtcag
     3001 gagtggagag aggatctgtc ttcaggttcc tcggccgggg cagctggagg tttcacaacc
     3061 cttgtctgca tgcccaatac ggatcctccg ctggacagcc cagccctggt aagttatgtc
     3121 ctggaaaaga gcggaaggtc ccccggttcg aggatcctgc ctgcaggatg cgtcagcagg
     3181 gagagaaagg gcaaggttct ggccgagatg ggcaagatgg ccaaggctgg tgctgttatc
     3241 ttcaccgatg acggaaaccc cgtgagcgat actggactcc tcagacttgc cctccagtac
     3301 agctcctggc tgggcgtcag gcttatggag catccagagg agaagtctct gaccaaagat
     3361 gcacaggtca acgagggtat ctgttcctcc ttctcggggc tcaaggggtg gccatcgtcg
     3421 gcggaggcct cagacatttc caggggcgtc tctctctgca gggatacagg ggtccccatt
     3481 cacttcaccc acgtcagcgc ggaggattct ctccgtgtcc tcagggaggc gaaagcatcg
     3541 aagttgcccg ttacattcga cgttaccccc caccacctct gtctttctga agagaatgtc
     3601 atcgagggag ggtatgacag tgtcttcaag gtcaatcccc ccctcaggtc tcgcagggac
     3661 agggatgccc tgtgggaagc cctcgcggaa ggcaccgtcg atgctattgc gacggaccat
     3721 gctccctggc acattgacga gaag
//
LOCUS       DAOPKQ010000136         3724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_27413_length_3724_cov_5.170619, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000136 DAOPKQ010000000
VERSION     DAOPKQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27413_length_3724_cov_5.170619"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..416)
                     /locus_tag="PK146_05390"
     CDS             complement(51..416)
                     /locus_tag="PK146_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPE91711.1"
                     /translation="MKGFEGREKENRELLILWTSGDREVALSMVFMYVLNAKTRGWWE
                     EIRLVVWGPSGRILAQDQDLQIYMEKIIEAGVRVEACRACTDSYGVSEKLEGMGIEVK
                     YMGEPFTEMLKRGEKVLAL"
     gene            complement(476..787)
                     /locus_tag="PK146_05395"
     CDS             complement(476..787)
                     /locus_tag="PK146_05395"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HPE91712.1"
                     /translation="MNFYILFGVVAVALLVIKGLFLCFVPFGKIPPFFERSFRYIPPA
                     VLAALIAPSVLYAKGPAGLEISPARLIAGIAAFAVAMKTRNILVAIATGMTLLWTFSF
                     I"
     gene            complement(784..1548)
                     /locus_tag="PK146_05400"
     CDS             complement(784..1548)
                     /locus_tag="PK146_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HPE91713.1"
                     /translation="MLERFYKPLGLAGEMTMTDVKKEFLRGARCTVPLFIGLVPFALV
                     LGVAANEAGLNAAQGTFFSLSMLAGTAQLAAVQLYGAGASAAIILFTAIVVNLRYSMY
                     SLSLFQILGEKTFLERLLAAFFLSDQSYVITMAERENSDDNPFLPAFFFGSSLAVYTV
                     WVSGIFLGFSLKAIIPAGLSLDFAIPLVFMFFLIPHLKGRDRQFSVLAGVVSSVVLVP
                     RLPLQSGLLASILIGIGSGMAAGAFIRGKESEVEGK"
     gene            1731..3119
                     /locus_tag="PK146_05405"
     CDS             1731..3119
                     /locus_tag="PK146_05405"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPE91714.1"
                     /translation="MKGSLRRLWVFLVLCIAVLSLSYPASALSPAQEYLLEGSYAVEQ
                     DGWTLVHLEGVPYRIGFQRGYLTAESASYLIISYLGPADDEYHRMSREIAGKYLWDRI
                     PKEYQDELLGIAAGLRAKGYDWDVWDVLAANAWADQGLYEDAWEEDAESRCSAFIATG
                     DATADGQIVMGHNTWCPYNEDFMYPIIYDVKPSKGFAFRYQGAGGTIWSGQDWYVNET
                     GLMVTETSLENDATDPEGIPVIVRIRTAVQYADSIDDFLGIMLEGNNGAYPNQWLIGD
                     AKTGEIASLQLGLHSWDLERTFNGFLYSSNHPSGVNFREEAACEEPDPMNYCYARYVR
                     WGQLKEEWYGKVDAEIGKIMLADHYDTYLGIEKPSARTICGHKEIETADPSERKPSGA
                     YDGKVTTSDMVLDGMSMWARWGHPCGEPFDAEKFMEDNPQWVDENDEFAIHGLRIFDS
                     DTPNPWTLVENF"
     gene            3232..3426
                     /locus_tag="PK146_05410"
     CDS             3232..3426
                     /locus_tag="PK146_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91715.1"
                     /translation="MGKRSFVLDENTELRKRAGELSLLAETSEVRQDWFFIAEEASRE
                     GDWLLSEYAYRKGLGLQVLW"
     gene            3426..>3724
                     /locus_tag="PK146_05415"
     CDS             3426..>3724
                     /locus_tag="PK146_05415"
                     /inference="COORDINATES: protein motif:HMM:NF036481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91716.1"
                     /translation="MGTPAPTSRKILYRASLSTPVPARFLSRPNRFKVIGETASGAVE
                     AYLPNPGRLWELLVPDAMLLLEKSSQKEGRRTAYTVIAVETPQGPVMLHTHRSNDA"
BASE COUNT          923 a         1057 c         1001 g          743 t
ORIGIN      
        1 agaaattcct tcattcgccc tcactccttc gaaataatgc tgaaagccat ctaaagtgcc
       61 agtacctttt cccctctttt cagcatttca gtaaaaggtt cccccatgta ttttacttcg
      121 atacccatcc cctcaagctt ttcagaaaca ccgtagctgt cagtgcatgc cctgcacgct
      181 tcgaccctga ccccggcttc gatgatcttc tccatgtata tctgcagatc ctggtcctgg
      241 gcaagaatcc tcccggaagg cccccataca acaagacgga tctcttccca ccagcccctg
      301 gtcttcgcgt tgagaacgta cataaagacc attgacaggg ccacctccct gtccccgctg
      361 gtccagagga tcagcagttc ccggttctcc ttttccctgc cctcgaatcc cttcaatgtt
      421 cccacctccc cgacaaaagt ggataatagt caaaggttcc ttgcgaacct caacattaaa
      481 taaatgaaaa ggtccaaagc agggtcatgc ctgtcgcgat cgcgaccaga atattcctcg
      541 tcttcatggc aacagcaaag gcagcgatcc ctgcaatgag cctcgcagga gaaatttcaa
      601 gccctgcggg acctttggcg tacaggactg aaggcgcgat aagggcggca agaacggctg
      661 gcgggatata tcggaacgac ctttcaaaga atggagggat cttgccgaag ggaacaaagc
      721 aaaggaacag gccctttatc accagaaggg ctacggcaac aacgccgaaa agaatataga
      781 agttcattta ccttccacct cgctctcctt ccccctgata aaagctcctg ctgccatacc
      841 ggagcctata cctatcagga tcgaggccag aagacccgac tgaagcggaa gtcgtgggac
      901 cagcacgacc gaggaaacga ctccggcaag gaccgaaaac tgcctgtccc tgcccttgag
      961 gtgggggatc aggaaaaaca tgaagaccag ggggatggca aagtccaggg agagcccagc
     1021 ggggataatg gccttcaggg agaagcccag gaagatccct gatacccata cagtgtagac
     1081 ggccagcgat gaaccgaaaa aaaatgctgg aagaaacggg ttgtcgtcgc tgttctctct
     1141 ttcggccata gtaatgacat aagactggtc cgaaaggaag aacgctgcca gaaggcgctc
     1201 cagaaaggtc ttctccccca ggatctgaaa gagagaaaga ctgtacatgg aatatctcag
     1261 gttgaccaca atggcggtaa aaaggattat cgccgccgag gcgccggctc cgtagagttg
     1321 gactgccgca agctgggcgg tcccggctaa catggagaga gaaaaaaaag taccctgggc
     1381 ggcgttaagg cctgcttcat tagctgccac tccaagaaca agggcgaagg gcacaagacc
     1441 aatgaagaga ggcaccgtac agcgggctcc cctgaggaat tcttttttta catcggtcat
     1501 agtcatctct cctgcaagac caaggggttt atagaacctc tcaagcatta taatcgatac
     1561 ccatccaagt atattcttcg tatgcctctt ctcccgcctt ctcaaggtga aaacattccg
     1621 acggcatgga gaatgtcatc atttcctttc ccgaagatcc ggccctttgg cccttttctt
     1681 aaaggggagt agaatgagaa ggaagatcaa atgcaaaggg agtggtcaag gtgaaaggtt
     1741 cgttgcggcg cttgtgggtc ttcctggtcc tctgcatagc agtcctttcg ctctcttacc
     1801 ccgcctccgc tttatcaccg gcccaggaat acctcctcga agggtcctac gctgtggaac
     1861 aggacggatg gacactggtc catcttgaag gtgtcccata caggataggt ttccagaggg
     1921 gatacctcac ggcggagtct gccagttacc ttatcatcag ctacctggga cccgcagacg
     1981 atgagtacca caggatgtcc agggagatcg ccgggaaata cctctgggac cgcattccca
     2041 aagaatacca ggatgaactc ctgggcatcg ctgccgggct cagggcgaag ggctacgact
     2101 gggacgtgtg ggatgtactc gccgccaacg catgggcgga ccagggcctc tatgaagatg
     2161 cctgggaaga ggacgcagag tcccgatgca gcgccttcat agccaccggc gatgctactg
     2221 ccgacgggca gatagtaatg ggtcacaaca cctggtgtcc ctacaacgag gacttcatgt
     2281 accccatcat ctacgacgtc aagcccagca agggtttcgc cttccgttac cagggagctg
     2341 gagggaccat atggagcggg caggactggt acgtcaacga gacgggcctc atggtgacag
     2401 agacgtccct tgagaacgat gccacagatc cggaggggat accggttatt gttcgcatcc
     2461 ggaccgctgt acagtatgcc gacagtatcg acgatttcct tggaataatg ctcgagggca
     2521 acaacggcgc ttaccccaac cagtggctga tcggcgacgc aaagaccggc gagatcgcct
     2581 ccctgcagct cggccttcac tcctgggacc tggagagaac cttcaacggg ttcctgtact
     2641 cctccaacca tccttcgggg gtcaacttca gggaagaggc ggcctgcgaa gaaccggacc
     2701 caatgaacta ctgctatgct cgctacgtcc gctggggaca gctcaaggaa gaatggtacg
     2761 gcaaggtaga tgcggagatc ggcaaaatta tgctggccga ccactacgac acctatctgg
     2821 gaattgaaaa acccagcgca aggactatct gcgggcacaa ggagatcgag accgccgacc
     2881 cgtctgagag aaagcccagc ggggcctacg acggtaaggt gaccacatcg gatatggttc
     2941 ttgacggaat gtccatgtgg gcccgctggg gtcacccatg cggagaaccc ttcgatgcgg
     3001 agaagttcat ggaagacaac ccgcaatggg tcgatgagaa cgacgagttc gccatccacg
     3061 ggctaaggat attcgactcc gataccccaa acccatggac tttggtggaa aacttttaga
     3121 cccgcccttc ttccattcgt cgagggggcg gctcaacgct gccccccttt actatctcct
     3181 ctttaggaac ccgggagacg gccgggttcc ctttcgtgct atcctggtag catgggaaaa
     3241 agatcctttg tcctcgatga gaacaccgag ctgagaaaaa gagcggggga actctccctg
     3301 ctggccgaaa cttcggaggt caggcaggac tggttcttta ttgccgagga ggcatcacgg
     3361 gagggcgact ggctgctctc cgaatacgcc tacaggaaag gcctggggct gcaggtacta
     3421 tggtgatggg aacccctgct ccaaccagcc ggaagatcct gtacagggct tccctctcga
     3481 cgccggtccc tgccaggttc ctctccagac ccaaccgctt caaggtgatc ggtgagacgg
     3541 cttccggagc ggttgaggct taccttccca accccggtcg gctgtgggag ctcctcgttc
     3601 ctgacgcaat gctgctcctc gagaagtcgt cgcaaaaaga gggccgcagg acagcctaca
     3661 cagtcattgc tgtagagacc ccacaaggcc cggtcatgct ccatacccat cgctcgaacg
     3721 atgc
//
LOCUS       DAOPKQ010000137         3704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_27626_length_3704_cov_4.838860, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000137 DAOPKQ010000000
VERSION     DAOPKQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27626_length_3704_cov_4.838860"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK146_05420"
     CDS             complement(<1..568)
                     /locus_tag="PK146_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE91717.1"
                     /translation="MWKGRLPRHILEMTHESYAKRAESQLHRSPGSLLVNCGLGTNPL
                     GVPPTVRRLLKSCHKWAICDYPPPVAKSLTGAIARYLGDEDLQDRIVLGHGSMDVLIT
                     LARLLLPPGSLLSGVSPQFTDMPLQAMQGNARYHPVILKGPLFEANLEIWKRVARQRP
                     HVLYIDRPHNPTGQVLPLEDLKLIVEESL"
     gene            627..805
                     /gene="ssrS"
                     /locus_tag="PK146_05425"
     ncRNA           627..805
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK146_05425"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            885..1859
                     /locus_tag="PK146_05430"
     CDS             885..1859
                     /locus_tag="PK146_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPE91718.1"
                     /translation="MMIAPPFWHPSVARGFILDWDGVLADTKLDFAPIRAKYFKGERV
                     PLVEAADMLEESRREDLERDLYELEMEGARGAEPVQGAHELLEWLRMWKIPWAVVSRN
                     CRDSIFEAAKRCSIELPETVMSRDEGPLKPDPQALWAAAGAIGVEPSLCLMVGDFIFD
                     LYGARRAGMRAVLVERVVPEWDKWADISFQRLEDLVRSLGSPEPMVPREYRELAGKRG
                     RKWLERAWEIDAALPEASPEIFAVALAAVGLGIGGLKLPEKARLSLEQWERAHFLPLS
                     LVDRPIVSVLTQVLGERFPQVNFSRNGASLELPGDPDLVEEAVLSWMK"
     gene            1889..3286
                     /locus_tag="PK146_05435"
     CDS             1889..3286
                     /locus_tag="PK146_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPE91719.1"
                     /translation="MRESVPSSKWEVPLAERMRPRILEEMAGQRHLISRGAPLYRAVQ
                     SGHVPSCILYGPPGVGKTSIARLMATMTERDFLEINAVSANVAALRDLVAEAKHLKNI
                     SGRTPVAFVDEIYHFNRQQQNALLPSVEKGDLILMGTTTENPSFEINKTLLSRAVVYE
                     LRPIETEDMVGLLERALSDIERGLGELELSASRETLTEIALGAGGDARQALTRLELSA
                     HAVATGGGSVIDIETVRRISPKALQRYDRDEDDHYRIISALIKSMRGSDPDAAVYWLG
                     RLVAGGEDIRFVARRLLVFAAEDVGLADPRALSVAASAAYAADFVGLPEARIILSEAV
                     IYLSLAPKSNSAYMAVDRALSAIEGGDLQEVPPHLRPGGKGYIYPHDLPGHWVAQRYM
                     EHPRTFYRPGSMGAEPEMVRLHELKRDGHYSGEYEDDTSGKAWGQPLPEEEGGEEGRK
                     EGPDGSDADHHVDGS"
     gene            complement(3200..3502)
                     /locus_tag="PK146_05440"
                     /pseudo
     CDS             complement(3200..3502)
                     /locus_tag="PK146_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
BASE COUNT          757 a         1036 c         1213 g          698 t
ORIGIN      
        1 caaggctctc ttcgactata agcttcaggt cttccagggg gagtacctgt cccgtggggt
       61 tatggggccg gtctatgtaa agtacgtgcg gacgctggcg ggcgaccctc ttccagatct
      121 ccaggtttgc ctcgaagagg ggtcccttca gaatcaccgg atggtagcgg gcattgccct
      181 gcatggcctg gagcggcatg tcggtgaact ggggtgagac cccggacaaa agggatcccg
      241 gaggcagaag caggcgggcc agggtgatca gaacgtccat tgaaccgtgc cccagaacta
      301 tccggtcctg aaggtcctcg tccccgaggt acctggcgat cgcccccgtc aggctcttgg
      361 ccacaggcgg agggtagtcg catatagccc acttgtggca ggatttaaga agcctcctga
      421 ccgttggagg taccccaagc gggttagtgc cgagtccaca attcaccagc agagaaccgg
      481 gggaacggtg aagctggctc tccgctcttt tagcgtagct ttcatgggtc atttccagga
      541 tgtgtctcgg gagccttcct ttccacaatt taaacccctc ctaacgactg ttatgatata
      601 ataccggtgt agcgggtaaa agagaacagg ccctgcggtg ttcgtgaaca tggtgaagag
      661 ccttgagcca acacccggtt atgggaagct tttctacccg ccggcagtgg cggcgctcag
      721 gcgctacccg cgcaaaggcg ggtgaggttt ccacctagtt aggatcgggt caaggccttc
      781 gtgtcacggc actcgcgggg gcgtcttttt tacacggagc ggggtatggg ggccaagggt
      841 cttcatgccc cgttttcttt aatcttttac cggaggatga tcccatgatg atcgcaccac
      901 ctttctggca cccctccgtg gcaaggggct tcatcctcga ctgggacggg gtcctggccg
      961 acacaaagct tgacttcgct cccataaggg caaagtactt caaaggcgag agggtccccc
     1021 tggtggaggc cgccgacatg ctggaggaga gccggcggga agatctggag agggacctct
     1081 acgaactgga aatggagggg gcaaggggtg cggagccagt ccagggagcc catgaactcc
     1141 ttgagtggct gaggatgtgg aagataccct gggcagtagt ctcccggaac tgcagggact
     1201 cgatcttcga ggcagcgaaa agatgctcca tagaactgcc cgagacggtg atgtccaggg
     1261 acgaggggcc cctgaagccc gatccgcagg ccctctgggc cgcagccggt gccattggcg
     1321 ttgaaccttc tctctgcctc atggtgggtg acttcatttt tgacctctac ggcgcacgaa
     1381 gggcggggat gagggcagtc ctggtggaga gagtggtccc cgagtgggac aagtgggccg
     1441 acatctcctt ccagcgtctg gaggacctcg tgaggtccct tggttccccc gagccgatgg
     1501 ttcccaggga gtaccgggaa ctggccggta agcggggaag aaagtggctt gaaagagcct
     1561 gggagataga cgcggcactc ccggaagctt ccccggagat cttcgctgtg gcactggccg
     1621 ctgtggggct gggtatcggg ggactgaagc ttcctgagaa ggcaaggctg tctcttgagc
     1681 agtgggagcg cgctcacttc ctgcctcttt cgctggtgga caggccaatc gtgtcggtgc
     1741 tgacccaggt cctgggagag cgattccccc aggttaattt ctcgaggaac ggggcgtccc
     1801 tggagcttcc gggcgatccg gacctggtcg aggaggctgt tctttcatgg atgaagtgag
     1861 gggtgagagg accctctggg acgatccgat gcgggagagt gtaccctcca gcaaatggga
     1921 ggtccccctg gcggagagga tgaggccgag gatcctggag gagatggcag ggcagaggca
     1981 tctcatatcc cggggggctc ccctctacag ggctgtccag tcgggacacg ttcccagctg
     2041 tattctctac ggcccgcccg gcgttggcaa gacctccata gcccgcctga tggcaactat
     2101 gacggagagg gatttcctgg agatcaacgc cgtctcggcg aacgtggcgg ccctgaggga
     2161 ccttgtggcc gaagcgaagc acctcaagaa tatcagcggc aggaccccgg tggctttcgt
     2221 ggacgagata taccacttca acaggcagca gcagaatgcc ctgctcccgt cggtcgagaa
     2281 gggcgacctg atcctcatgg gcactactac agagaatccc tctttcgaga tcaacaagac
     2341 gctcctctca agggcagtag tttacgagct tcgtcccatt gagaccgaag acatggtggg
     2401 gcttttggag agagccctgt cagatatcga gagggggctc ggagagctgg agctctccgc
     2461 ttccagggag accctgacgg agatcgccct cggcgccggc ggtgacgccc ggcaggccct
     2521 gacaaggctg gagctatccg cccatgccgt tgccacaggg ggcggaagcg tgatagacat
     2581 cgaaacggtg aggagaatat cccccaaggc actgcagcgc tacgaccgtg acgaggacga
     2641 tcactacagg atcatttcag ccttgatcaa gagcatgcgg ggctccgacc ccgatgcggc
     2701 ggtctactgg ctgggacggc tcgtggcagg tggggaggat atccgttttg tcgccaggcg
     2761 cctcctcgtg ttcgccgccg aggacgtggg ccttgccgat cccagggccc tttctgtggc
     2821 tgcctccgcg gcttacgccg cggacttcgt tggacttcca gaggccagga taattctctc
     2881 cgaggcagtg atatacctgt cgctggcccc caagagcaac agcgcctaca tggccgtcga
     2941 cagggcgctg tctgccatag agggggggga ccttcaggag gttcccccgc acctgaggcc
     3001 cggcggaaag gggtacatct acccccatga tcttccgggg cactgggtgg cacagaggta
     3061 tatggaacat cccaggactt tctacaggcc gggttcgatg ggggctgagc cggagatggt
     3121 aaggctacac gaactaaaac gagatggcca ttactccggc gaatacgagg acgatacctc
     3181 cggcaaggcg tggggtcagc ctctcccgga ggaagagggt ggcgaagagg gccgcaagga
     3241 agggcctgat ggaagcgatg cagaccacca cgtcgacggg agctagactg tagctgtagg
     3301 tcaggaagag gttcccgacc gtaaggccca gcgcacccgc gatggccatc tcggtcatcg
     3361 ccacccgcga gacgttagca accccccttg ccctttcaag caggaagaac atgatccatg
     3421 aacccgtcgt aacgctcact gcccgccacc actccagctg aggcaccggg atggtctgca
     3481 tgagcacctt gttggttatc atcccgaagg tccagcatga catggcaagg aatgagacca
     3541 aaaggcctcc gaaaatctgc cgtttcccct cggggttgtc ttcgcggggt gacaggagta
     3601 taaggcccgc cacgataagg acagccgcgg caaataccat ggggcttgcg ggttcgccca
     3661 ggaagatcca tgaggcaggt accaccagaa gagggtagga ggaa
//
LOCUS       DAOPKQ010000138         3704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_27630_length_3704_cov_3.818306, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000138 DAOPKQ010000000
VERSION     DAOPKQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27630_length_3704_cov_3.818306"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..482
                     /locus_tag="PK146_05445"
     CDS             87..482
                     /locus_tag="PK146_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPE91720.1"
                     /translation="MSEEKSEGSVRISEEVIAQIATQALLKVNGVQPASPGLVANLRL
                     GKKTSGGVRISVEEGDMPSVVVDAYITTKYGLRIPDIAWDVQESIKKTLEQYTGYVVK
                     AVNVFVQGVFFEHEQTTFKEPVEEKKEAE"
     gene            649..1020
                     /locus_tag="PK146_05450"
     CDS             649..1020
                     /locus_tag="PK146_05450"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPE91721.1"
                     /translation="MFQGSDLEKGRPDPGVGHRAERGEDEMKISRDVIQQVANQTLLG
                     VIGVRAADTEGKDKDAVKIDITEGPVPSIRVDTAIKVKYGLRIPDIAWYVQESIKRGL
                     EQNTGYTVEEVNVFVKGLYRE"
     gene            1045..1410
                     /locus_tag="PK146_05455"
     CDS             1045..1410
                     /locus_tag="PK146_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPE91722.1"
                     /translation="MDQKRSEVSDPGKIRISEDVVEQIAVQALAKVIGVRPAEPGASE
                     GTGIGRRPVDGVSISFEEGESPAIVVDAFIRTKYGLRIPDICWYVQESLRSTLEQFTG
                     YDVKSVNVFTQGVYFDEQS"
     gene            1492..2094
                     /locus_tag="PK146_05460"
     CDS             1492..2094
                     /locus_tag="PK146_05460"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPE91723.1"
                     /translation="MSASKIFGSFEESHTPTDLQDKKKMKFSTDGRVRISEEVIAELA
                     SQALMKVIGVQPDQGEGGTRLAQGVDIMVEEEGGPSVVVDAYIRTKFGLMIPDIAWYV
                     QESIRNSLEQNTGYNIKAVNVFVKGLYFEDVKKPVLEMTIKEKVQPSPPVEIVTPPVE
                     ARKSTVEEKKEEAPAGAPTEEQAPKKEKTSIFGKIEKNKP"
     gene            2169..2654
                     /locus_tag="PK146_05465"
     CDS             2169..2654
                     /locus_tag="PK146_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91724.1"
                     /translation="MYYFWRSGEKGELWLSGGGIRDAYSRMYPDGPVCSEVSLLADRD
                     MLNVSFALEPGLSPSDKSASEKRFREFARELGFSDIQAGWIREDRLQEALTASTIVRL
                     PLTWGVAVWVLVAVIRMGVSGTLLATAFGLAAFSVAALYTTERGSEIRRWFRKLSEKR
                     P"
     gene            2673..3137
                     /gene="nusB"
                     /locus_tag="PK146_05470"
     CDS             2673..3137
                     /gene="nusB"
                     /locus_tag="PK146_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPE91725.1"
                     /translation="MKAIVPQKRRRSREIALQILYSMEMRENISPVAAVELYPFDSED
                     EEVKSYAARLVLGVSESRNIVDDLIRRHIIGWRPERMVIVDRVAIRLALYESIVARLI
                     PVPVAISEAVELAKHFGTAESGKFVNGVLGKIVRVLDRGEGTESPDAGDTDR"
     gene            3115..>3704
                     /gene="xseA"
                     /locus_tag="PK146_05475"
     CDS             3115..>3704
                     /gene="xseA"
                     /locus_tag="PK146_05475"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPE91726.1"
                     /translation="MPGILTVDELTRYISLLFEESETLSSLQVRGTLSGFKRHSSGHV
                     YFTLLGEESRISCALFRSDAGRIPLWPADGDEVLVTGKAGVYGPRGAYQIYARTIRPL
                     GEAAQTRAKKELMERLKREGLFEPALKRRLPPLPLRVALVTSPTGAAVRDVVKVARER
                     FPLCRLLVVPTVVQGATAVEEIARGLVRAGSIPEVEA"
BASE COUNT          865 a          947 c         1191 g          701 t
ORIGIN      
        1 tgagcccatc caggaaagaa cctgacaagg aaatacccgt tgacagcacc gtagggagtc
       61 ctgtcgagcc cgggaacgac cccgggctgt cagaggagaa gagcgagggg agcgtcagga
      121 tctccgaaga ggtcatagcc cagatagcca cccaggccct gctcaaggtg aacggcgtcc
      181 agccagcgag cccaggcctt gtggcgaacc tgaggctcgg caagaagacc tccggcggcg
      241 tcaggatctc tgtcgaggag ggcgatatgc cctcggtggt ggtcgacgcc tacatcacaa
      301 ccaagtacgg tctgaggatc cccgacatag cctgggacgt acaggaatcg atcaagaaga
      361 cccttgaaca gtatacgggg tacgtcgtga aggccgtaaa cgtctttgtc cagggggtct
      421 tcttcgaaca cgaacagacg acgttcaagg aacctgtgga ggagaagaag gaagcagaat
      481 gaatctgtcg ggtggagcta ccctttgccc gattcaggcc agtggctctg ccggtccctg
      541 aagggtcctg ctatgggaga gttcgtggga gcctggagat ggttggaaat gatctgcatt
      601 taatataaac tttaacggtg cttaaacatc tcggggggtg gcttgaatat gttccagggg
      661 tctgatctcg aaaagggaag acccgatcca ggggtcggcc atagggcgga aaggggcgag
      721 gatgagatga agatttccag ggatgttatc cagcaggtgg cgaaccagac tctcctcgga
      781 gttatcggcg tcagggcagc agacacggag ggcaaggata aagacgcagt aaagatcgac
      841 atcaccgaag gtcctgtccc ctctatccgt gttgatacgg ccattaaggt caagtacggc
      901 ctccgtatac ccgatatcgc ctggtatgtc caggagtcca taaagagagg gctcgaacag
      961 aacacaggat acaccgtgga ggaagtgaac gtattcgtaa aagggcttta cagggagtga
     1021 tccttttcat ctggagggga tgacatggac cagaagaggt ctgaagtgtc cgatccggga
     1081 aagatcagga tatccgagga tgtggtggaa cagatagccg tccaggctct ggcaaaggta
     1141 attggggtca gaccggcgga gcccggggct tccgaaggaa caggaatagg cagaaggccc
     1201 gtcgacgggg tcagcatatc cttcgaagag ggcgaatcgc ccgctatagt cgtggatgcc
     1261 ttcatcagaa cgaaatacgg actcaggatc cccgatatct gctggtacgt ccaggaatcg
     1321 ctgagaagta cgctggagca gttcaccgga tacgacgtca agtccgtgaa cgtctttacc
     1381 cagggtgtct acttcgacga acagagctga catgaaaggg tccggagggg agccggcccc
     1441 ggaccgccgt aacgacctta ttgtaaaaaa agcgaaagga gtggatcata catgagtgca
     1501 tcgaagatct tcggctcctt cgaggagagc cataccccga cggatctcca ggataaaaag
     1561 aagatgaagt tcagcactga cggaagggtc aggatatccg aggaggtaat tgccgaactg
     1621 gcatcgcagg ccctcatgaa ggttatcgga gtgcagcccg accagggcga gggtgggacg
     1681 cgtcttgccc agggagtgga cataatggtc gaggaagagg gcggcccctc tgtggtggtc
     1741 gacgcgtaca tcaggaccaa gttcggtctc atgatccccg atattgcctg gtatgtgcag
     1801 gagtccatca ggaactccct tgaacagaac acaggctaca acatcaaggc cgtcaacgtc
     1861 ttcgtcaagg ggctttattt cgaggacgtc aagaagcccg tactcgagat gaccattaag
     1921 gaaaaggtcc agccttctcc ccccgtcgag atcgttaccc ctcctgtgga ggcccggaag
     1981 agcaccgtgg aggagaagaa ggaagaggct cctgcgggtg cgccgaccga agagcaggcc
     2041 cccaaaaagg agaagaccag cattttcggg aagatagaga agaacaagcc ctgatcgtac
     2101 tggcccaggc cggttatcaa gcggccggtc ccatcggggc cggcctttta tgtcgtgggg
     2161 tgatctgatt gtactacttc tggagatccg gtgaaaaggg ggagctctgg ctgtccggag
     2221 gaggtatccg ggacgcctac tccaggatgt accccgatgg ccctgtttgt tccgaggtgt
     2281 cgctccttgc cgaccgggac atgctgaatg tctcgtttgc actggagccg gggttgtcac
     2341 cgtcggacaa gtccgcctcg gagaagaggt tcagggaatt tgcccgggaa cttggcttct
     2401 cggacatcca ggcagggtgg atcagagagg accgtcttca ggaagcgctg acggcctcga
     2461 ccattgtgcg gctccccctg acctggggtg tcgcggtatg ggtcctggtg gcggtgatcc
     2521 gaatgggtgt ctcggggacg cttctggcta ccgcctttgg gctggctgcc ttctccgttg
     2581 cagcgcttta caccacggag cgggggagtg agatccgccg gtggttccgg aaactttcgg
     2641 aaaagagacc gtaaaggaag ggcagtggca atttgaaggc aattgtcccc cagaagagaa
     2701 gaagatcgag agagatcgca ctccagatcc tctattccat ggagatgcgc gagaacatct
     2761 ctccagttgc ggcggtggag ctctacccct tcgactccga ggatgaagag gtgaagagtt
     2821 acgccgcaag gctggtcctg ggcgtctcag agagccggaa catcgtagat gacctgatcc
     2881 ggagacatat catcggatgg aggccggagc ggatggtcat cgtagacaga gtggcaataa
     2941 ggctcgctct ctacgagagc atagtcgcca ggctgatccc cgttccggtg gccatctcgg
     3001 aggccgttga actggcaaag cacttcggga ccgccgaatc gggcaagttt gtgaacggcg
     3061 tactgggcaa gatcgtccgg gtcctggaca ggggagaagg aacggagagt cccgatgccg
     3121 gggatactga ccgttgatga gctgacccgt tacatttcac ttcttttcga ggagagcgag
     3181 accctttcat ccctccaggt cagggggacc ctttcggggt tcaaacggca ctccagcggg
     3241 catgtctatt tcacactgct cggggaggag tcaaggatat cctgcgctct tttcaggtcc
     3301 gatgccggac ggatccccct ctggcccgca gacggtgacg aggtccttgt gacaggcaag
     3361 gcaggggtct acggtcccag gggggcctac cagatctacg ccaggaccat taggcccctg
     3421 ggtgaggcag ctcagaccag ggcgaaaaag gagctgatgg aacgtctcaa gagggagggg
     3481 ctctttgaac ccgcccttaa gagaaggctc cccccgctcc ccctcagggt agcccttgta
     3541 acctccccta cgggtgcagc tgtcagggat gtagtaaagg tggccagaga gaggttccct
     3601 ctctgcagac tcctggttgt cccgacggtt gtccaggggg cgaccgccgt agaggagatc
     3661 gcaagaggcc tggttcgtgc cgggagcatc cccgaagtcg aagc
//
LOCUS       DAOPKQ010000139         3650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_28195_length_3650_cov_3.617246, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000139 DAOPKQ010000000
VERSION     DAOPKQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28195_length_3650_cov_3.617246"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PK146_05480"
     CDS             complement(<1..810)
                     /locus_tag="PK146_05480"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPE91727.1"
                     /translation="MTVEVLKYGGSSLAGDGRLDSVAGDIAAIAEGGVSVAVVVSAVG
                     HTTDSLLETAGRVNGRTNRREIDQLLATGEQQSVALLTMALEKRGIRARSFSGWQAGI
                     RGQGVFTDGRIRGVVPSAVQKALERGEVAVVAGFQAVTDDGDVITLGRGGSDLSAVAL
                     AAALEADCCRIFTDVDGIYSADPGMVPSAVRLESVSCGECMEMAAAGARVMQARSVEL
                     AWRYDVPLFVASSSDRERGTWIVREDVSEKFVVRAIAHDSGVAKVAVLGVPD"
     gene            complement(807..1751)
                     /gene="thrB"
                     /locus_tag="PK146_05485"
     CDS             complement(807..1751)
                     /gene="thrB"
                     /locus_tag="PK146_05485"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPE91728.1"
                     /translation="MRSDSLRIRVPATTANLGSGFDTIGLSLSLFNIYDVFDIDEEGA
                     YRMEVIGEGSAELSDPESNMIIQSYERACREWGIPCPGFTLRCLNAIPLCRGLGSSSG
                     AVAGGVLIANFFREKPLAMEELLPLMVEIEGHPDNIVPACLGGLVISCLEGGKLRYVK
                     LPSPPKDIFAVVAVPDVRVRTEDARKALPQEVSLGDAVFTLNRAALLAASWASGKWEN
                     LSWAMDDRLHQRFRAKLFPGGEVILGKVKEVPDCLGAAISGSGPSVLAFAHGSPRGVA
                     EAMCRVFREHGVRSRFFVLDVDEEGASIERRSGEVDAA"
     gene            complement(1748..2788)
                     /gene="thrC"
                     /locus_tag="PK146_05490"
     CDS             complement(1748..2788)
                     /gene="thrC"
                     /locus_tag="PK146_05490"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPE91729.1"
                     /translation="MRGVLERYKDLLPLNGSTPMVSLREGSTPLIPLDSIGGELGVRL
                     YAKYEGANPTGSFKDRGMVLAVAKALEAGARGLVCASTGNTSASAAAYAARSGLPCVV
                     LLPAGNVAMGKLAQAMIYGAKAVSVRGNFDEALQLALRVAEDSGYAVVNSVNRYRLWG
                     QRSGAWEICDELGHSPDRLILPVGNAGNITAYWAGFVQYDRIGKSDGIPVMTGVQAEG
                     ASPMVRGEPFDSPETVATAIRIGRPVSARRASWAARMSGGSFLAVSDEEILQAQKKLA
                     SREGVFAEPASCAGIAGLYRLAGEGCLPEGEEIVTILTGNGLKDPDAVLAGLPMPMEA
                     EADVRSIREVLA"
     gene            3119..3277
                     /locus_tag="PK146_05495"
     CDS             3119..3277
                     /locus_tag="PK146_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91730.1"
                     /translation="MEGFVKDSRESINQTTGNWRLEAGRAKKPLDVRRETNGKTRRLE
                     EGIWRLGT"
BASE COUNT          725 a         1267 c          951 g          707 t
ORIGIN      
        1 atccggaacc ccgagaacgg ccacctttgc tacccccgag tcgtgggcga tagcccttac
       61 gacgaatttt tccgaaacgt cctctctcac gatccatgtc cccctttccc tgtcagaact
      121 cgaggcaacg aagagcggga catcgtacct ccaggccagt tcaacgctcc tggcctgcat
      181 aaccctggct ccggcggccg ccatctccat gcactccccg caacttacac tctcaagcct
      241 caccgccgac ggcaccatgc ccggatcggc ggaatatatt ccgtccacgt cggtgaatat
      301 cctgcagcag tccgcctcca gcgcggcagc cagtgccacc gccgagaggt ccgacccgcc
      361 tcttccgagg gtgatcacgt ccccgtcgtc ggtaactgcc tggaaaccgg cgacaacggc
      421 cacctcgcct ctttcaaggg ctttctgaac cgccgacgga accacgcccc gtatccttcc
      481 gtcggtaaag accccctgcc cccttatacc agcctgccat cccgagaagg agcgggccct
      541 gatgccacgc ttctcgaggg ccattgtcag cagggccacg ctctgctgct cccccgtcgc
      601 cagaagctgg tctatctccc tgcggttcgt ccttccgttg acccttccgg ccgtctccag
      661 cagagaatcg gtggtatgcc ccacggcgga aaccaccacg gcgacggaga cgcccccttc
      721 ggctatagcg gcgatgtccc cggcgaccga gtcaagcctc ccgtcaccgg caagggacga
      781 tccaccgtat ttcaggactt cgacggtcac gccgcatcga cctccccgct tcgtctttcg
      841 atacttgcgc cctcctcgtc cacgtcgaga acaaaaaagc gggaccttac cccatgttca
      901 cggaaaaccc tgcacattgc ctcggcgaca cccctgggag atccatgggc aaaggccagg
      961 acactgggac cggacccgct tatggcggcg ccgagacaat ccgggacctc cttcaccttt
     1021 cccaggatca cctcgccccc gggaaaaagt ttggcccgga acctctggtg cagcctgtcg
     1081 tccatcgccc acgagaggtt ctcccacttt cccgaggccc atgaagccgc aagcagcgcg
     1141 gccctgttca acgtgaaaac ggcatccccc aggcttactt cctgcggcag ggccttcctg
     1201 gcgtcctccg tccgcaccct gacatcgggc acggccacga cggcgaaaat gtcctttggc
     1261 ggagagggga gtttcacata ccggagtttc ccgccttcca ggcaactgat caccagtccc
     1321 ccgagacatg ctggcactat attgtcggga tgtccctcga tctcgaccat gaggggaagc
     1381 agttcctcca tggcaagcgg cttctcccgg aagaagttgg caatgaggac ccctcccgcc
     1441 accgctcccg aggaactccc cagccccctg cacaggggga tggcattgag acacctcagg
     1501 gtgaaaccgg gacaggggat accccactcg cggcaggccc tctcgtaact ctggattatc
     1561 atgttcgact ccgggtccga gagttcggcg cttccttcac cgataacctc catcctgtat
     1621 gccccctcct cgtcgatgtc gaatacgtcg tagatgttga aaagggagag agaaaggcct
     1681 atcgtgtcga aaccggagcc caggtttgcc gtcgtcgcgg gcacccttat cctcaggcta
     1741 tctgatctca cgccagcacc tcccttatgg accgcacatc cgcttcggcc tccatgggca
     1801 tgggcagccc ggcaaggacc gcgtcggggt cctttaggcc gttgccggtc aggatagtaa
     1861 caatctcttc accttcggga agacatccct ccccggcaag ccggtaaagc cccgctatgc
     1921 ccgcgcacga ggcaggttcg gcgaagacgc cctcacgaga ggcgagtttt ttctgagcct
     1981 gcagtatctc ttcatccgac acggcaagaa aactcccccc gctcatccgt gctgcccacg
     2041 aagcccttcg ggcgctcacc ggtcgcccta tgcgtatagc cgttgccacc gtttcgggag
     2101 agtcaaaggg ctcacccctc accatggggg aagccccctc ggcctgtacc cccgtcatca
     2161 cgggaattcc gtcgcttttc cctatgcggt catactgaac gaaccccgcc cagtaggcgg
     2221 tgatgttacc cgcgttcccc acggggagga tcagcctgtc cggggaatgt ccaagttcgt
     2281 cgcagatctc ccaggctccg ctcctctgtc cccacaaccg atagcgattg acggaattga
     2341 ccacggcata gccggaatcc tccgcgaccc gaagagccag ttgcagggcc tcgtcaaaat
     2401 ttccgcgcac cgataccgct ttggccccgt aaatcatcgc ctgggcaagt ttgcccatcg
     2461 ccacgtttcc ggcggggaga aggacgacac agggaagacc gctcctggcc gcataggccg
     2521 ctgccgatgc cgaagtattg cccgtggatg cgcagacaag ccctctcgcc cccgcctcaa
     2581 gagccttcgc caccgccagg accattcctc tgtccttgaa ggaccccgtc gggttcgccc
     2641 cctcgtactt ggcgtacagc ctcacgccga gttcgccacc gatggaatca agggggataa
     2701 ggggcgtgct cccctcccgc agcgaaacca tcggggtcga cccgttaagc ggaagcagat
     2761 ccttgtagcg ctcaagaact cccctcattg caacccctcc caaaatgatg ttccaaaata
     2821 aaaagcccct ttccatctcc tgctacagga gacgaaaagg ggcgcttttc gcggttccac
     2881 tccagttccg gccttggccg gctctcgtac ccgctgttag gggcggaccc cgtcacctta
     2941 tcccgtaaag ggcttcggat cccgctccgg agtggtcttc tccgcaccct gcgccgggga
     3001 ccttctcagc ctgtgagacc ccctctctgt cacgcgatct gctgcggata ctatctccat
     3061 catcgccgtt gccatattgc cgctacgttc cctgcggttt ccgaacttta tagacctgat
     3121 ggaaggattt gtcaaggaca gccgtgaatc gataaaccag acgacaggaa actggagact
     3181 ggaggcagga agggcaaaaa agccgttaga cgtgagacgt gagacgaatg gaaaaacaag
     3241 gagactggaa gaaggcatct ggagactggg aacctgaaag gcaagggcaa gtgcttgtca
     3301 ttctgaggaa tgaaatgacg aagcggaatc cgccagtagg cggatcagaa tgacaaatct
     3361 tgggtccccg gttcccacca aaaaggtttt ctctgcgggg ccccaccctc tgtgtcccct
     3421 gtggtgagaa gggccggggt gaaccctcct gcactgtgtt cttttcctgc ccctacgcgg
     3481 cggctgcgaa actcaccggc tcctgagcct tcggtaaagc cttcactccg gcacttgccc
     3541 ttgctttccc cccgtctcac gtctcacgtc tcacgtctca cgctcttgat ccattcgatt
     3601 cacgatttac ggcacctgac ccagattctt cgctccgctc agaatgacaa
//
LOCUS       DAOPKQ010000140         3640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_28287_length_3640_cov_4.418689, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000140 DAOPKQ010000000
VERSION     DAOPKQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28287_length_3640_cov_4.418689"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PK146_05500"
     CDS             <1..1464
                     /locus_tag="PK146_05500"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPE91731.1"
                     /translation="NDKTTTSVGLGQGLAAIGKKAMIALREPSLGPSFGVKGGAAGGG
                     YSQVVPMEDINLHFTGDIHAVTTAHNLVSAMIDNHLQQGNELGMDPRRIVWRRVLDLN
                     ERALRSVIVGLGGKPHGVPRESGFDITVASELMAILCLSTDLMDLKERVGRIIIGYTY
                     DNRVVKAGDLGVAGSVAVLLKDAIKPNLVQTLEGVPAFVHGGPFANIAHGCNSIQATR
                     HGLKLADYFVTEAGFGAELGAEKFFDIKCRMAGLRPDAVVLVASVRALKMHGGLPKGD
                     LSRENVDALKSGLPNLEKHIENLKSFGVPVVVAVNRFPTDSQEELDAVMERCKALGAS
                     FSLSEVWGKGGQGGIDLARKVVEACTKSNGFRFLYDAEASPREKIETIARGLYGAGDV
                     QYTDAAEKDLASIHELGLDHLLVCMAKTQASISDDPAKVGRPDGFTLTVREVRVSAGA
                     GFIVPITGSIMTMPGLPKKPAALDIDIDAEGRITGLF"
     gene            1488..2345
                     /gene="folD"
                     /locus_tag="PK146_05505"
     CDS             1488..2345
                     /gene="folD"
                     /locus_tag="PK146_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPE91732.1"
                     /translation="MSNAKVLDGRRTAAELRAAIGVKVRDLQGECRRVPGLAVVLVGD
                     DPASKVYVGQKEKACREAGIRSFLHHLPASTSTSTLTELLEDINKDPDVHGMLVQLPI
                     PGHIDWTEAVRSIDVRKDVDGTHPLNLGRLVMGLDTVEPCTPRGVMYLLKKYGIDLSG
                     KRAVVLGRSNIVGKPMAQLLLAADATVTVCHSKSIDIETMTREADIIIAAIGRPEFLK
                     ASMVKPGCVVVDVGISRLDGGLVGDVDFQDVSRVASWITPVPGGVGPMTIAMLLQNTL
                     ERYLELECA"
     gene            complement(2342..>3640)
                     /locus_tag="PK146_05510"
     CDS             complement(2342..>3640)
                     /locus_tag="PK146_05510"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HPE91733.1"
                     /translation="HLATHDTLTGLTNRTLLLDRLEQALQFASRSGRPLAVLLINLDR
                     FRMINDSLGSTVGDGLLMAVASRLVESVREGDAVARMGGDNFAILLTSMARQADALAV
                     GAKILDQLSLPFSVLGRKIRISASIGVSVFPGEGKDPETLVKHAELAMFEAKKVGGKS
                     LKAFETGMDTRMTESHLLEDGLHSALERTEFVLFYQPQVDIKTGRITGAEALIRWQHP
                     ERGLLPPGSFIPIAEETDLIVRIGEWVLREACEQASKWTAIGLPPILVSANISARQFR
                     EKGLLEKMQKTIKRSGVNPGRICLELTESVLMEDPFAAIETIRSLKETGICLALDDFG
                     TGFSSLNYLRRFPVDTLKIDRSFISDLLTDPGAAGVINSVISLARHLGLMTLAEGVET
                     REQLEMLSRWGCDSYQGFLCSRPVPAGEFEQLLRKDGNPL"
BASE COUNT          786 a          988 c         1121 g          745 t
ORIGIN      
        1 aatgataaga cgaccacctc cgtggggctg ggccaggggc ttgccgcaat aggcaagaag
       61 gcaatgatag ccctgaggga gccgagcctc ggtcccagct tcggggtcaa gggaggcgca
      121 gccggtggcg gttactccca ggtggtaccc atggaagata ttaaccttca cttcaccgga
      181 gatatccatg ctgtcacaac cgctcacaac cttgtgtcgg ccatgataga caaccacctc
      241 caacagggga acgaactggg tatggaccct cgcaggatag tctggagacg ggtcctggac
      301 ctgaacgaaa gggccctgcg gagtgtgata gtcggtctcg gcgggaaacc gcacggtgtg
      361 ccccgggaga gcggttttga cataacggtg gcgtctgagc ttatggcgat actctgcctg
      421 tccacggacc ttatggacct caaggagcgg gttggcagga tcatcattgg ctatacctat
      481 gacaacaggg tcgtcaaggc aggcgacctg ggtgtggcag ggtccgtggc ggtcctgctg
      541 aaggacgcga tcaagccaaa cctggtccag accctggagg gagtgcctgc cttcgtccac
      601 ggaggcccct ttgcgaacat agctcacggc tgcaacagca ttcaggcgac aaggcacgga
      661 ctcaagcttg ctgactattt cgttaccgag gccggtttcg gggccgaact tggggccgag
      721 aagttcttcg acatcaagtg ccggatggcc ggattaaggc ctgacgcggt tgtcctggtg
      781 gcatcggtga gggctctaaa gatgcacggg gggctgccga agggcgatct ctccagggag
      841 aatgtggatg cactcaagtc gggtcttccc aacctggaga agcacatcga gaacctgaag
      901 tcctttggag tacccgttgt ggttgctgtt aaccggtttc cgacggattc acaggaggag
      961 ctcgatgctg tcatggagag gtgtaaggct ctcggagcgt ccttctccct gtccgaggtc
     1021 tggggcaagg gcgggcaggg gggcattgac cttgcgagga aggtcgtcga ggcctgcaca
     1081 aagagtaacg gcttccgttt cctatacgac gcagaggcct ccccgcggga gaagatcgag
     1141 acaatagcca gggggcttta cggcgccgga gatgtccagt acacagacgc cgcagaaaaa
     1201 gacctggcca gcattcacga actggggctg gaccacctgc ttgtctgcat ggccaagacc
     1261 caggcttcca tatcggacga ccctgcgaag gtgggacgcc ccgatggctt caccctgacc
     1321 gtacgggaag tccgcgtctc tgccggagcc ggtttcattg tcccaataac aggaagcata
     1381 atgaccatgc caggccttcc caaaaaaccc gcagcactgg acatcgacat cgatgcagag
     1441 ggcaggataa cggggctctt ctagctatca gggaggtctc aggtgctatg agcaacgcga
     1501 aagtgctgga cggcaggaga acggccgcag aattgagggc ggccattgga gtaaaggtgc
     1561 gggatctgca aggagagtgc aggagggttc ccggtctcgc ggtcgttctt gtcggcgacg
     1621 atccggcctc gaaggtttac gtaggccaga aggagaaggc ctgccgcgag gcaggcatca
     1681 ggtcctttct ccatcatctt ccggcatcaa cgagcacttc caccctgact gaactgctgg
     1741 aggacatcaa caaggatccc gatgttcatg gaatgctcgt ccagcttccg atcccgggac
     1801 acatcgactg gaccgaggcg gtacggtcaa tagatgtaag aaaagatgtg gacgggaccc
     1861 acccgctgaa cctgggacgc cttgtaatgg gtctggatac agtcgagcct tgcactcccc
     1921 ggggagtgat gtatcttctg aagaagtacg gcatcgatct ttccgggaag agggcggtag
     1981 ttctcggcag gagcaacatt gtgggcaaac ccatggcaca gcttctgctt gcggcagatg
     2041 ctacggtaac ggtatgtcac agtaaatcca ttgacattga aacaatgacc cgggaggcgg
     2101 atataataat cgccgccatc gggaggccgg agtttttgaa ggcttccatg gtgaaaccag
     2161 gctgtgtcgt ggtcgacgta ggaattagca gacttgacgg ggggcttgtg ggagatgtcg
     2221 acttccagga tgtctcccgg gttgcctcct ggataacacc cgttccgggc ggagttggcc
     2281 cgatgacaat agcaatgttg cttcagaaca cccttgagag gtatcttgaa ctggaatgtg
     2341 cctagagtgg gttcccgtct tttcggagaa gttgctcgaa ctccccggca ggaacgggcc
     2401 ggctgcaaag gaacccctgg tagctgtcgc agccccagcg ggagagcatc tcaagctgct
     2461 ccctggtctc gaccccttcg gcaagggtca tcaggccaag gtgtcgggcc agggatatta
     2521 cactgttgat gaccccggca gccccggggt ctgtcagcag atcggagata aaggacctgt
     2581 ctatcttgag ggtatccaca gggaatcgcc taaggtagtt caggcttgag aaacctgttc
     2641 cgaaatcgtc gagggcaagg catatgcccg tttccttgag ggaccttatg gtctcaatgg
     2701 cagcgaaagg atcctccatc agaacgctct ccgtaagttc caggcatatc cttccgggat
     2761 tgacgcccga tctttttatg gtcttctgca tcttttccag cagtcccttt tctcggaact
     2821 gcctggccga gatgttggcc gagaccagta tgggaggaag tccaatggca gtccattttg
     2881 aggcctgttc acaggcctcc cgcaggaccc actcgccgat ccggactatc aggtctgtct
     2941 cctcggctat cgggataaag gaacccggag ggaggagtcc ccgttccggg tgctgccagc
     3001 gaataagggc ctcggccccc gttatccttc ccgttttaat atcgacctgg ggctgataga
     3061 aaagaacgaa ctcagttctc tccagggcac tgtggaggcc atcctcgagg agatgcgact
     3121 ccgtcatgcg ggtgtccatc ccggtttcga aggctttcag acttttacca ccaactttct
     3181 tggcctcgaa catggccagt tctgcgtgct ttacaagcgt ctcggggtcc tttccctccc
     3241 cggggaagac ggataccccg atgctggcag aaatgcgaat tttcctgcca agaacactga
     3301 agggcaaaga cagttggtca aggatcttcg cccccacggc aagcgcatcg gcttgcctgg
     3361 ccattgatgt cagcaggatc gcgaagttgt caccgcccat tctcgccacg gcgtctcctt
     3421 cacgcacgga ctccaccagt ctgcttgcca ctgccatgag gaggccgtcg ccaacggtac
     3481 tacccaggga gtcgttgatc atcctgaaac ggtcgaggtt gatcagaagc acggcaaggg
     3541 gtctcccgga acggctcgcg aactggagag cctgttcgag tcgatccaga agaagggttc
     3601 gattggtcag cccggtgagt gtgtcgtggg tcgccaggtg
//
LOCUS       DAOPKQ010000141         3632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_28386_length_3632_cov_4.391389, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000141 DAOPKQ010000000
VERSION     DAOPKQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28386_length_3632_cov_4.391389"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PK146_05515"
     CDS             <1..226
                     /locus_tag="PK146_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91734.1"
                     /translation="LCMASNTPGWGSVYRVALREIMGLDIPVLNLGPSGKDPHRPTER
                     LCLSYSLEVFPILLREAVVSLGLSHPMLTP"
     gene            246..743
                     /locus_tag="PK146_05520"
     CDS             246..743
                     /locus_tag="PK146_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HPE91735.1"
                     /translation="MNGNKSVREGAGTPSVRLVRAALIGGVYAALTLIFAPLSFGPMQ
                     VRVSEALTLLPWIWAEAIPGLFVGCLISNFVGGFGIIDMVFGSAATLTAAILTSRMPN
                     RYLAAVPPVAVNALVVGGYLSVLLDIPAVPTMIYVGTGQMIACFGLGIPLLSLFERRF
                     RRGGC"
     gene            730..2913
                     /locus_tag="PK146_05525"
     CDS             730..2913
                     /locus_tag="PK146_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE91736.1"
                     /translation="MEDVNRFFNVDAFYETLIDQAPLGIAIQLTDGRVIRANDSFCKM
                     FGYTMDEVVGGQLDDLVALEGDIYAEAGDVTKRVVHGENIFIEAVRTRKDRSRIPLSI
                     WGVPIREGGRTLAVYAIYEDISERKAAEKDLRRERALLERVLVDSPDGIVLFDEEGVV
                     FRTNPAFEHLFGLDPGEALGHSLWEVVGRGEKDADIRDNIKKLRDKKRVDHEAVRVRK
                     DGSKVHLSIRGAAISRFDSSGEYLAIYRDITERIRSEQELANERIYFENLFMNSPLAI
                     ALVCDDGVIQRVNESFEKLFGYSSWECVGMNLDDLIAPGEARDDAEKLTRDVAAGSIL
                     KTERTRLRKDGSRVDVQINAVSFPVAGGQKVVYAIYQDITERKILDEKIRHFGYHDAL
                     TGLYNNAFLEEELRRLDTSRQLPISLIMADVDNLKLINDAFGHMEGDQLIIEAGAILR
                     SCCRQEDLIARCGGDEFIMLLPRTSLLEARSICDRIRQACDRASKGMIPPSIALGVAA
                     KDEVTQDFMQVRKKADDDMYLDKLMRSERSRSAIYSRIVDFLGSDPRRRAHISRLKKL
                     AESFGTFLALRQDEIENLDLLAQFHDIGLMPIPTDVLYREGPLDPEEWEDVRKHPERG
                     YHLARNLAPISPVVEDILCHHEHFDGSGYPRGLSGEDIPRPSRIIHLLCAYEVMTGWR
                     SYRQALSPEEALEEIASQAGKQFDPRMAGLFIKLHRTMELDGRLI"
     gene            complement(2906..>3632)
                     /locus_tag="PK146_05530"
     CDS             complement(2906..>3632)
                     /locus_tag="PK146_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE91737.1"
                     /translation="KELVTLRRRCGYVPQDPFGCVPPTLNALDAAAEPIRITGKGNRE
                     KALGKARELLAELGLDDQDLWKERVRLSLSGGQRQRVSIARALSLDPELLLADEPASM
                     QDAANRGEVMEILRRRTRKGMGLLMATHDLPFAAAFADRVVVMYKGTVVETGPPDQVV
                     ENPLHPYSLALISAMPGIGKGIRPPRPVEVKDRSFGGCPFAPMCPTRTERCRQAPSLT
                     TAWPSRSVACWNVGKESLERLAR"
BASE COUNT          752 a         1032 c         1085 g          763 t
ORIGIN      
        1 gctctgcatg gcatccaaca ctcccgggtg ggggagtgtc tacagggttg ctctaaggga
       61 gatcatggga ctggatatcc cggtacttaa tctcggtccc tcagggaagg atcctcacag
      121 accgaccgag aggctttgcc tctcctactc cctggaggtt ttcccgatcc tgctgcgcga
      181 ggcggtagtg tccctggggc tgtcgcaccc gatgttgaca ccttgaaggg gagttggatc
      241 ctttcatgaa cggcaacaaa agcgtcaggg agggggcggg caccccctcg gtacgccttg
      301 tcagggctgc cttgattggc ggtgtttatg cggccctgac cctgatcttc gccccgttgt
      361 ccttcggtcc gatgcaggtt agggtctccg aagccctgac gctccttccg tggatatggg
      421 ccgaggcgat cccaggtctt ttcgtgggtt gcctcatctc caattttgtc ggcggtttcg
      481 gtataatcga catggtattc ggaagtgcgg caacactaac cgctgccata cttacctcca
      541 ggatgcccaa ccggtacctc gccgccgtac ccccagttgc cgtcaacgcc ctcgttgtag
      601 ggggctatct ttccgttctc cttgatattc ctgcagtccc gaccatgatc tatgtcggta
      661 caggtcagat gatcgcgtgc tttggtctgg ggatacctct tctctcgttg ttcgaaaggc
      721 ggtttcgccg tggaggatgt taacagattt tttaacgtcg atgctttcta cgagactctt
      781 attgatcagg ccccgctggg tattgccatc cagctcacgg acggtagagt gatccgtgcg
      841 aacgattcct tctgcaagat gtttggttat accatggacg aggttgtggg gggccagctt
      901 gatgacctcg tcgcacttga aggggacatc tacgctgaag ctggggacgt gacaaaaagg
      961 gtcgtccacg gagagaatat cttcatcgag gcggtccgga cacgaaagga ccgttccagg
     1021 atcccactct ccatatgggg tgttccaatc agggagggtg gaaggaccct ggcggtatac
     1081 gccatctacg aggacataag cgaacgaaag gcagcggaga aggatctcag gcgagaaagg
     1141 gccctccttg aaagagtgct ggtagattca ccggacggaa tagtcctctt cgatgaggag
     1201 ggggtagtct ttcgtaccaa tcccgccttc gaacatctct ttggactgga cccgggggaa
     1261 gccctggggc attcactctg ggaggtcgta ggcagagggg aaaaggatgc cgatattcgc
     1321 gacaacatca agaagctgag agataagaag agagtggacc acgaggctgt cagggttcgc
     1381 aaggacggca gcaaggtcca cctgtcaata aggggggcgg ctatatcccg tttcgactcc
     1441 tccggggaat atctcgccat ctacagggac ataaccgaga ggataaggtc ggaacaggaa
     1501 ctggccaacg agaggatcta cttcgagaac ctcttcatga acagtcccct ggccatagcg
     1561 ctcgtatgcg atgatggcgt catccagcgg gtcaacgagt cctttgaaaa actgttcgga
     1621 tacagcagct gggagtgcgt cgggatgaac ctggatgatc tcatagcgcc gggagaggcc
     1681 cgggacgacg ccgaaaagct gaccagggac gttgcagccg gatccatcct caagaccgaa
     1741 aggaccaggc ttagaaagga tggaagtcgg gtggatgtcc agatcaatgc cgtgtcattc
     1801 cccgtcgccg gagggcagaa agtggtgtac gccatatatc aggacataac cgagagaaag
     1861 atcctcgacg agaagatccg gcacttcggc taccatgacg ccctgacagg actttacaac
     1921 aacgccttcc tcgaagagga actcaggcgc ctggacacct caagacagct ccctatcagc
     1981 cttatcatgg ccgacgtgga caacctgaaa cttatcaacg atgccttcgg tcatatggag
     2041 ggcgaccagt tgataattga ggccggggcg atcctgcggt cctgctgtag gcaggaggac
     2101 ctgatcgccc gctgcggcgg cgacgagttc atcatgctcc tgccaaggac gtccctcctg
     2161 gaggcaaggt cgatctgtga ccgcataagg caggcctgtg atcgtgcctc gaaggggatg
     2221 atccccccga gcatcgccct tggtgttgcc gccaaggacg aggttaccca ggatttcatg
     2281 caggtcagga aaaaggccga tgacgacatg tacctggaca aactgatgcg gagcgaaagg
     2341 tcccgttcgg ccatatattc ccggatcgtg gacttcctgg gctccgaccc caggagaagg
     2401 gctcatattt caaggcttaa gaagctggcg gagtccttcg ggacttttct tgccctccga
     2461 caggacgaga tcgaaaacct tgaccttctc gcccagttcc acgacatcgg gctgatgccg
     2521 ataccgacgg atgttctcta ccgcgagggg cctcttgacc ccgaagagtg ggaggatgtc
     2581 cgtaaacacc ctgaaagagg ttaccacctt gccaggaacc tcgccccgat atctcccgtt
     2641 gtcgaggaca tcctgtgcca tcacgagcac tttgatggat cgggctaccc cagggggctt
     2701 tccggggagg atatcccccg accttccagg atcattcacc tgctgtgcgc gtacgaggtt
     2761 atgaccggtt ggcgttccta caggcaggct ctatcccccg aggaggctct cgaggagatc
     2821 gcctcccagg ccggaaagca gttcgatccc cgcatggccg ggctcttcat caagctccat
     2881 aggaccatgg agcttgatgg ccgccttatc tagctaacct ttcaagggac tctttcccga
     2941 cgttccagca ggcaacactc ctcgaaggcc aggccgtagt caaagagggt gcctgcctgc
     3001 accgctccgt ccttgtcggg cacatcgggg cgaaggggca gcccccgaag gaccgatctt
     3061 tcacctccac aggccggggg ggtcttatcc ccttcccaat gccgggcatg gcggagataa
     3121 gggcaaggct gtaggggtgg agggggtttt cgaccacctg gtcagggggg cctgtctcga
     3181 ccacggtgcc tttgtacatg accacaaccc tgtctgcaaa ggctgccgcg aaggggaggt
     3241 cgtgggttgc catgagaagc cccatcccct tcctggtcct tcggcggagg atctccatta
     3301 cttcgcccct gttggccgca tcctgcatgc tcgccggttc gtcggccaga agtagctccg
     3361 gatcgagcga gagggccctg gctatgctga ccctctgtct ctgcccaccc gaaagggaaa
     3421 gcctgaccct ctccttccag agatcctggt cgtcaaggcc caactcggcg aggagctccc
     3481 tggcctttcc cagtgccttc tccctgttcc ctttgccggt gatccggatg ggttcggcag
     3541 cggcatcaag ggcattcagc gtgggaggaa cgcatccgaa ggggtcctgg gggacgtagc
     3601 cgcagcgcct ccgtagcgtg acaagctcct tg
//
LOCUS       DAOPKQ010000142         3627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_28440_length_3627_cov_4.553191, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000142 DAOPKQ010000000
VERSION     DAOPKQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28440_length_3627_cov_4.553191"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1532
                     /gene="hslU"
                     /locus_tag="PK146_05535"
     CDS             114..1532
                     /gene="hslU"
                     /locus_tag="PK146_05535"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPE91738.1"
                     /translation="MTGHRDEGLTPKSIVAFLDRYIVGQDKAKRAVAIALRNRICRRR
                     LPEEMAREIAPKNILMVGPTGVGKTEIARRLSHLVHAPFIKVEATKFTEVGYVGRDVE
                     SMIRDLVERSVQMVKERKVEEVREEAAVRARERILDALLPPRKVQEKVGDFMHIFSGQ
                     PQRQDPPPTEDDERAERQRLSTRERLRNMLDEGKLEGREIEIETSESPQLGIPVMGGP
                     GMEEMGMNIGEMLSGIFPKKKKRRTVTVEVARKILEAEEAERLVDHEAAAQEALDKAQ
                     QEGIVFIDELDKVASAGGGHSGPDVSREGVQRDLLPIVEGCSVNTKYGPVRTDHILFI
                     AAGAFHKMKPSDLVPELQGRLPLRVELNPLSEDDLARILVEPEHSLIDQYRALLSTED
                     VTLSFTDGAIKEIAMMAAKMNRETEDIGARRLHTIMENLLEEIQFEAPDMAGQEAKVE
                     EGTVRMKLEKLVSDSDVRKYLL"
     gene            1588..2418
                     /gene="codY"
                     /locus_tag="PK146_05540"
     CDS             1588..2418
                     /gene="codY"
                     /locus_tag="PK146_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HPE91739.1"
                     /translation="METPKELIESRAKIANPSAMEDLLVKTRAVNRVLQSRKDPNTPD
                     YQKVAKMLCELSAANVYMINRDGKILGYGWISEYDCPIMAELLETGFMPESYVEKLNQ
                     YHESELNHTDHGLCAYSDVACTYSNKHVVYIPIYGAGERLGTLVLARFGCEFDNRDLV
                     LGEYLATVVGLEILHARTRSIEERARERLIVQMAMRALSYSEVESVRHIIRELNGHEG
                     IVVASRVADRVGVTRSVIVNALRKLESAGIIESRSLGMKGTFIKVMSPLFLEDLGVSE
                     "
     gene            2521..2937
                     /locus_tag="PK146_05545"
     CDS             2521..2937
                     /locus_tag="PK146_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPE91740.1"
                     /translation="MVQDKGSQATEVKVGDIVKGTVEQILPYGAFVRLPTGQKAMIHI
                     SELAYEYVKKVEDILQLQQEVEAKVIKIDERSRIDLSVKQLQARAERPRQQRGPVSAA
                     DDFEKKLASFMKASDQKLTDLSRKKENTGGKPRGKK"
     gene            2973..3539
                     /locus_tag="PK146_05550"
     CDS             2973..3539
                     /locus_tag="PK146_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HPE91741.1"
                     /translation="MPPVFFDALLPGDLPVLKRQSGSRYFDPTIVAAVSRRCRHGFPQ
                     VTACCPLRDGRPFPTIFWLTCPFLSRAIDTLESSGGVREMGEVISCGKEEEWLLFNRV
                     HSLARVFLLGPEMAKRLMEEDPPAWDALVSTGAGGIRPKKDVSVKCLHLQAASMIGLG
                     WHPAGDWLMERLGEMECRDPGVWPCGQA"
BASE COUNT          883 a          890 c         1194 g          660 t
ORIGIN      
        1 taccttgact caaccgatgg aagggcagat gagatagcaa aaaaatccct ggcgattgct
       61 gccgggatat gcatatacac caacgatacg ataacgctgg aggtgctgga gggttgaccg
      121 gacacaggga tgaggggctg accccgaaaa gcatagttgc attccttgac aggtatattg
      181 tggggcagga caaggcaaag agggccgtcg ccatagccct tcggaacagg atatgccgcc
      241 gcaggcttcc cgaggagatg gcccgggaga tagccccgaa gaacattctc atggtggggc
      301 cgacgggcgt aggaaagact gagatagccc ggaggctttc ccacctggtg catgccccgt
      361 tcatcaaggt ggaggccacc aagttcaccg aggtcggata tgtgggcagg gacgtggagt
      421 ccatgatacg ggacctcgtc gaaagatccg tgcagatggt aaaggaacgc aaggtcgagg
      481 aggtcaggga ggaggccgcg gtaagggcga gggagaggat acttgacgcg ttgctgcctc
      541 caaggaaggt ccaggagaag gttggcgact ttatgcatat tttctccggc cagccccaga
      601 gacaggatcc ccccccgacc gaggatgacg aacgcgccga aaggcagaga ttgtccaccc
      661 gggagaggct caggaacatg ctcgacgagg ggaagctcga gggtcgggag atcgagatcg
      721 agaccagcga gagtccccag ctcgggatcc cggtcatggg cggaccggga atggaggaga
      781 tgggcatgaa catcggggag atgctgagcg gcattttccc caagaaaaag aagcgcagga
      841 cggttactgt ggaggttgcc aggaagatac tggaggccga agaggccgag aggctcgtag
      901 accacgaggc cgccgcccag gaggcgctgg acaaggcaca gcaggaagga atagtcttta
      961 tcgacgaact cgacaaggtc gcgtccgccg ggggagggca ctcggggccg gatgtcagca
     1021 gagagggtgt ccagagggat cttcttccca tcgtggaggg gtgctccgtc aacacgaagt
     1081 acggtccggt aaggaccgat cacattctct tcattgccgc cggggctttc cacaagatga
     1141 aaccttccga cctggtaccg gaactgcagg ggcggctgcc gctgagggtg gaactcaacc
     1201 cactctcgga ggatgacctg gcccggatac tggtggaacc ggagcacagc ctgatcgacc
     1261 agtacagggc gctgttgtcg acggaggatg taaccctgtc ctttaccgac ggcgcgataa
     1321 aagagatagc catgatggcg gctaaaatga acagggagac cgaagacatc ggcgccagga
     1381 ggctccacac cataatggag aatctcctgg aggagataca gttcgaggcc ccggatatgg
     1441 cgggtcagga agcgaaagtg gaggaaggga ccgtgagaat gaaactggaa aaactggtct
     1501 ccgactcgga tgtaaggaag tacctgcttt gagcgtggct attgccggca aggcgattgt
     1561 gtctacaggg agggatcgga catgacaatg gagacaccga aggaacttat agaatcgagg
     1621 gccaagatcg cgaatccctc ggctatggag gacctcctgg tgaaaacgcg ggcggtaaac
     1681 agggtgctcc agagcaggaa ggacccgaac accccggact accagaaagt cgcaaagatg
     1741 ctctgcgaac tgtccgcggc gaacgtctac atgataaacc gcgacggcaa gatcctgggt
     1801 tacggatgga tcagcgagta cgactgtccc ataatggcag aactcctgga gacgggtttc
     1861 atgccggaga gttatgtgga gaaactgaac cagtatcacg agtcggaact gaaccacacg
     1921 gaccacggcc tctgcgccta ctcggacgtg gcctgcacct actcgaacaa gcatgtcgtg
     1981 tatattccga tatacggtgc gggggagaga ctgggaaccc tcgtcctggc caggttcgga
     2041 tgcgagtttg acaaccgtga tcttgtcctg ggagaatacc tcgccactgt tgtggggctg
     2101 gaaatacttc atgccaggac caggtccatc gaggagaggg cgagggagag gctgatagtc
     2161 cagatggcca tgagagccct ttcatactcg gaggtcgagt cagtaaggca tataatcagg
     2221 gaactgaacg gccacgaagg gatagtcgtg gccagccgcg tcgcggacag ggtaggagtc
     2281 acaaggagcg taatagtaaa cgccctgagg aaactggaga gcgcggggat aatcgagagc
     2341 aggagccttg gtatgaaggg gaccttcata aaggtcatga gcccgctttt cctggaagat
     2401 ctgggcgttt cagagtagtg gcgggtaatg ctatacttgt ccagccatac cggtccgaag
     2461 ggtccggtga atttatagat catctattca gtgaagacag atggagggga aaggtactcg
     2521 atggtccagg ataagggctc ccaggcgacg gaagtgaaag tcggcgacat tgtaaagggc
     2581 acggttgaac agatccttcc ttacggggct ttcgtccggc tcccgactgg gcagaaggcg
     2641 atgatccata tctccgaact tgcctacgag tatgtaaaga aggtggagga catcctgcag
     2701 ttgcagcagg aggtcgaggc aaaggtcatc aagatcgacg agaggtcgag aatagacctt
     2761 tcggtgaagc agctgcaggc ccgggcggaa cgcccgagac agcagagggg cccggtttcc
     2821 gcggccgatg attttgagaa aaaactggct tcattcatga aggcgagcga tcagaaatta
     2881 acggacctga gcaggaaaaa ggaaaacacc gggggaaagc cccgcgggaa aaagtagttt
     2941 cttcaaagaa gtctcccggg acagggggcc tgatgccccc tgtttttttt gatgcccttc
     3001 ttcccggcga cctgccggtc ctgaagagac agtccggctc gcgttatttc gaccccacaa
     3061 ttgtggcggc cgtttcaaga aggtgccgtc acggttttcc ccaggtgacc gcctgttgcc
     3121 ccctgagaga tgggcgacca ttccccacga tcttctggct gacatgtcct ttcctgtccc
     3181 gtgctatcga cacgctggaa tcctcgggag gggtcaggga aatgggggaa gttatttcct
     3241 gcggcaaaga ggaggagtgg cttcttttca accgcgtcca ctctctggca agggtgtttc
     3301 tgctcgggcc ggagatggcg aaaagactca tggaagaaga tcccccggcc tgggatgccc
     3361 ttgtgtcaac cggtgcgggg ggtatacgcc ccaaaaagga tgtatcggta aaatgtctcc
     3421 acctgcaggc ggcttcgatg atcggtctcg gctggcaccc tgcgggagac tggctgatgg
     3481 aaagactcgg cgagatggaa tgccgtgacc cgggggtttg gccttgcggg caggcctgat
     3541 gtttggagcc atagactgcg gttccaactc ggtccgctgc ctggccgtcc ttcatgggga
     3601 cgggatcctg gaatatgtcg attcggg
//
LOCUS       DAOPKQ010000143         3622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_28497_length_3622_cov_4.044575, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000143 DAOPKQ010000000
VERSION     DAOPKQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28497_length_3622_cov_4.044575"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PK146_05555"
     CDS             complement(<1..561)
                     /locus_tag="PK146_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HPE91742.1"
                     /translation="MTLAIALGVTIPITPAQVLWVNMVTAVTLALSPAFEPMESNVME
                     RPPRSPKEPLVDRLMLVRISFVSFLLMASSLGLFSSYISAGASTELSRTIAVNTLIIG
                     EVFYLFNSRTNGRALSVKGLMATPKVWTAAVAVILLQIPFTYLPSMQGLFGTEAISLG
                     DWLLIFSLGIAVFLLVEAEKALFRTPP"
     gene            complement(558..2702)
                     /locus_tag="PK146_05560"
     CDS             complement(558..2702)
                     /locus_tag="PK146_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPE91743.1"
                     /translation="MTLSARDHAVVPMEEIKEQFHSMSPEEVLEALSSGPEGLSEEEA
                     RKRLDEFGPNRIPEPKKQNVLLRFFKHFHDVLIYVLLISAIATALLGHWIDTWIILAV
                     VILNSFVGFVQEGRAEKAIEAVRRMLSVSSSVSRDGKKRQIPAEELVPGDIVLLSSGD
                     KIPADLRLIRIHELQVNEASLTGESDTVSKTTETVKAEAALGDRTCMAYSGTIVVSGT
                     ARAIVISTGENTEIGKINRMLAEVEPLATPLFRKVNSFGIKLSIFILAMAGAVFLYGS
                     LVMGMPFNEIFLFVVAIAVAAIPEGLPAVISIILAIGVRRMAGRNAIIRRLPGVETLG
                     SVTVICTDKTGTLTKSEMTVSKVVIGNGLIEIDGAGYGPEGKIRRAGEEMDGGRDTAL
                     SRLVLAASLCNGSRILKKDGSWVSEGNPTEGSLLTLALKAGLDPEEASRQWQRLDTIP
                     FESEHKFMATLNHSGEEKRIFLEGAPESVIARCSAEMDQEGKTRELDAERWASVQEDL
                     AGEGFRLLAIADKDAPDDLGDDLDMDDVKEDFTLLGVAAIMDPPRPEAIDAVEECHRA
                     GIRVKMITGDHLLTATVIAKQMGIGNSDRVMTGPEIEDASDEELGNLLQDTDIFARVS
                     PEHKLRIVSSLQERGHITAMTGDGVNDAPALKKADIGIAMGIKGTEAAKEASEMVLTD
                     DNFASIVHAVEEGRTVYDNLKKTLIFMLQPSL"
     gene            complement(2744..3052)
                     /locus_tag="PK146_05565"
     CDS             complement(2744..3052)
                     /locus_tag="PK146_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HPE91744.1"
                     /translation="MNYLTGFLNFDLFIGTTVIKVLYFAGAFLVPLFLWLVLQRGRAR
                     CPRIEGLLSLLAEKTGERAALWLIFFLSLVALEIIWRVACEYLIAYLQIRQILQVLSG
                     "
     gene            3246..>3622
                     /locus_tag="PK146_05570"
     CDS             3246..>3622
                     /locus_tag="PK146_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE91745.1"
                     /translation="MFEIRWHGRGGQGCFTVARLLGMAASVHEGKYSLAFPSFGPERR
                     GAPVLGFTRISVTPITDRSMVDQCDYVVILDETLVGPSSLAGIRDGGVLVINTKVPES
                     YVPMTNRLIVGIDATSIAMDVLG"
BASE COUNT          802 a          969 c         1018 g          833 t
ORIGIN      
        1 aggcggggtc ctgaacaagg ctttctcggc ctccaccaga aggaagacag caatacccag
       61 agagaatatc aggagccagt ccccaagcga gatcgcttct gtcccgaaaa gcccctgcat
      121 cgacggaaga taggtgaagg ggatctgcag gagtataact gccaccgcgg cggtccagac
      181 ctttggggtc gccattaatc ccttgaccga aagggcccgg ccgttggtcc tgctgttgaa
      241 tagatagaag acctccccga ttatcagcgt gttgacagct attgttcgag agagctccgt
      301 tgatgccccg gcagagatgt atgaggagaa caaccccagc gagctggcca tgagcaggaa
      361 cgaaacgaag gaaattctca ccagcatgag cctgtcgaca aggggttcct tcggcgatct
      421 cggtggtctt tccataacgt tcgattccat gggttcgaag gcaggagaga gggccagggt
      481 aacagcggtg accatgttga cccacagtac ctgggctgga gttatcggta tggtcacccc
      541 cagtgcgata gccagggtca caaggagggc tggagcatga agataagggt cttcttcagg
      601 ttatcgtaga ccgtgcgacc ctcttcaacg gcatgtacta tcgaggcaaa attgtcgtcg
      661 gtaagaacca tttctgaggc ttctttggct gcctcggtgc ccttgatgcc catggcaatg
      721 cctatgtcag ccttcttgag ggctggcgcg tcgttgaccc cgtcgccggt catggcggta
      781 atatgccccc gttcctgaag ggaggacacg atccggagtt tatgctccgg cgagacccgg
      841 gcaaatatgt cggtgtcttg cagaaggttc cccagctctt cgtcggaggc gtcctcgatc
      901 tccgggcctg tcattacccg gtcgctgttc ccgataccca tctgttttgc tataactgtg
      961 gcagtaagaa ggtggtcccc tgtgatcatc tttaccctga tccctgccct gtggcactct
     1021 tcgaccgcat caatggcttc cggtctgggg gggtccatta tcgcggccac tcccaggagg
     1081 gtgaagtctt ccttcacatc atccatgtcc aggtcgtcac ccagatcatc aggagcgtcc
     1141 ttgtctgcga tagcgaggag acggaatccc tctccggcaa gatcctcctg aaccgaagcc
     1201 cagcgttcag cgtcgagttc acgggttttg ccttcctggt ccatctcagc tgaacatctt
     1261 gcaatgactg attcgggcgc tccctcaagg aagatccttt tctcttctcc tgaatggttg
     1321 agggtggcca tgaatttgtg ctccgattcg aaggggatcg tgtctagtcg ctgccactgg
     1381 cgggaagcct cttcagggtc caggccagct ttcagcgcaa gagtgagaag ggacccctca
     1441 gtcgggtttc cctcggaaac ccaggagcca tccttcttca aaatcctgga tccgttacaa
     1501 agtgatgcgg caaggacaag tcttgagagg gcagtatctc tcccgccgtc catctcttca
     1561 cctgccctcc tgatctttcc ttccggtccg tagcctgccc cgtcgatctc gatgagcccg
     1621 ttaccaatga ctactttcga gacggtcatc tcgctctttg tcagggtgcc ggtcttgtct
     1681 gtgcatataa cagttaccga tcccagggtc tccacccccg ggagtcggcg gataattgca
     1741 tttcttcctg ccattctcct cactcctatg gcgaggatta tactgatcac cgctggcaga
     1801 ccttcaggaa tggcggccac cgcaatggcc acgacgaaga ggaagatctc gttgaagggc
     1861 attcccatga ccagggatcc gtacagaaag actgctcctg ccatggccag gatgaaaatg
     1921 ctcagcttga tcccgaagct gtttaccttg cggaagaggg gagttgcaag aggctccact
     1981 tccgcaagca ttctgttgat ctttccgatc tcggtgttct ctccagtaga tattacgatg
     2041 gctctggccg taccggagac aacgatcgtc ccggaatagg ccatgcaggt cctgtccccc
     2101 agggctgcct cggctttaac ggtctcggtg gtcttggaga cagtatccga ttctcctgtg
     2161 agagaagctt cgttgacctg gagctcgtgg atcctgatca gcctcaggtc tgccggtatt
     2221 ttgtcccctg atgacaaaag gacgatatct cccgggacaa gctcctcggc ggggatctgt
     2281 cttttcttgc cgtcacggga aacgctgctt gacacggaaa gcattctccg tacggcttcg
     2341 atcgcttttt cggctcttcc ttcctggacg aagcctacaa aagaattgag aatgaccacg
     2401 gcgagtatta tccaggtgtc gatccagtga ccaaggagag cggtagcgat ggcggagatg
     2461 agcagaacgt atataaggac atcgtggaaa tgcttgaaga aacgcaagag gacgttctgc
     2521 tttttgggct caggaatccg gttaggtccg aattcgtcca ggcgcttgcg tgcttcctct
     2581 tcggatagac cttccggacc cgaagataat gcctccagca cttcttcggg tgacatggag
     2641 tggaattgtt cctttatttc ttccataggg acaacggcat ggtccctggc cgataaagtc
     2701 aagcctgggg aggaaggatg tggctgaaga ccgggaaagg agttcagccc gacaggacct
     2761 gaagtatctg cctgatctga agataggcga tcaggtattc gcaggcgacc ctccagatga
     2821 tctccagggc aacaagggat agaaaaaaga tgagccagag agcagctctc tcccctgtct
     2881 tttcagccag aagggacaag aggccctcaa tccgggggca gcgcgcacgg cccctttgaa
     2941 gtaccagcca gaggaaaaga ggcaccagga aggctcccgc aaagtagagg acctttatca
     3001 ctgtcgtgcc gatgaaaagg tcaaaattga gaaaaccggt aagatagttc aagcaggtca
     3061 ttctccttgt caagaggtgg tgatccctgt ccattctatc ccaatcccgg tcttaactgt
     3121 gggaagggga ggacccccgg gaaccctgaa aggcacaatg aggagcgctg ccctgaccgg
     3181 gttcccgaaa gtggtgatat agttttaaaa aagatatgct tctgacagag cagggaggtc
     3241 ctgatatgtt cgaaataagg tggcatggac gtggaggcca ggggtgtttc actgtggcga
     3301 gactcctggg gatggcggct tctgtccatg aaggaaagta ctctctggca ttcccttctt
     3361 tcggacccga gagaagagga gccccggtcc ttggtttcac caggatcagc gtaacaccca
     3421 taactgaccg tagcatggtc gatcaatgcg actatgtcgt gatccttgac gaaactctgg
     3481 tggggccatc ttccctggcc ggcatcaggg acggaggggt tcttgtcatc aataccaagg
     3541 ttcccgaaag ttatgtccca atgacaaaca ggcttatagt gggtattgat gccacgagca
     3601 tcgccatgga tgtcctgggg ag
//
LOCUS       DAOPKQ010000144         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_28963_length_3583_cov_3.933390, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000144 DAOPKQ010000000
VERSION     DAOPKQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28963_length_3583_cov_3.933390"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PK146_05575"
     CDS             <1..539
                     /locus_tag="PK146_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201292.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HPE91746.1"
                     /translation="GVDINVLIYQIPGGMYSNLVSQLKEQNSLDRLQDVLREVPIVRK
                     EMGYPPLVTPTSQLVGTQATLNVLMGERWKMVSKEVKNYFLGYYGTPPGPLDREVQKK
                     VIGDEKAITCRPGEVLEPELPGVYEAMGSWIMQPEDALSYALFPAVAKDFLVRKFARE
                     NLRDVGLEPLVEGAAYPL"
     gene            583..819
                     /locus_tag="PK146_05580"
     CDS             583..819
                     /locus_tag="PK146_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPE91747.1"
                     /translation="METEERARSLINEQIRPALQEHGGDIEIVGFDPDGSVLTVRLKG
                     ACGGCPFAAETLRTQVEAVIRKEIPEIEKVVRIQ"
     gene            816..1826
                     /locus_tag="PK146_05585"
     CDS             816..1826
                     /locus_tag="PK146_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPE91748.1"
                     /translation="MKHDRAGRTAGDRVISDRSCSVEASNEETLSRLLGPRDENLRLI
                     EERYPVRLAVKDLLVSVQGPDTEVVDLVSELIRQLMGIARKGHVLRPAEVTYAMNVFA
                     EEGVIDLLPMYEDVICLTYRGKPVRPKTGGQKRYVDAIRRNDVLFAVGPAGTGKTYLA
                     VALAVAALKEGKVNRVVLVRPAVEAGESLGYLPGDLKEKVEPYLRPLYDAFYEMFSPE
                     RFMKYVEKKVIEIAPLAYMRGRTLNDSFIILDEAQNTTPEQMKMFLTRLGFGSKAVVI
                     GDITQIDLPSGKDSGLKVVRNILKRIPGVEFVSLDKNDVVRHEVVQRIVQAYEEYEQK
                     RQ"
     gene            1807..>3583
                     /locus_tag="PK146_05590"
     CDS             1807..>3583
                     /locus_tag="PK146_05590"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPE91749.1"
                     /translation="MSRNGSKGPALSRIGLYAEKMASDRVYRLRWTATFLLAAVASSL
                     LAGTWWVRDGRSSFRIGSPAPQTFYAHSRMRIVDENATRALREQRMSDVGGVLVRDRR
                     FPEEVKSKLASIESGEYRTVLPESLAGILESLPEISAGRIATVSSRVAISMLSFPMME
                     GSPAETIWKGLEASDLSIPEQNLAFQILDHIMERAMVMDPEMTSEVRVLAASEIEPVE
                     RVLAVGDVIVREGETVSAELALVLRAQGYPELSFPWKTLTFVVLGVILWASWMWWYSV
                     RREFNFDSLEWGFIITILIAGWVAMFVSALLGENGLGVISLAGWAYLGLSGSFAFHLV
                     LWGGIIGSVITSGSSTVGLMLSVFASGITASLGYILMRKITSRLDLVRKMFLLGFFLA
                     FGAFFIGWGLYLPSDRSILVTQIVSAFAWVMVALVALPLWERFFDILTPIRLVDLTHP
                     SHPLLKRLQIEAPGTYHHSLMVGTLAEAAAGKLKMNPLLVKAGASFHDVGKLRRPQFF
                     VENQQTGHNPHDELAPTLSALIIISHVREGLETARQFGIPKKIRVFIKEHHGTTCLHY
                     FYKKALSLDPDLPMDQFCYPGDRPRS"
BASE COUNT          787 a          970 c         1060 g          766 t
ORIGIN      
        1 aaggggtcga tatcaacgtc ctcatctacc agataccagg cggcatgtac tccaaccttg
       61 tgagccagct gaaggagcag aactccctgg accgactcca ggacgtgctc agggaggttc
      121 ccattgtccg caaggagatg ggctacccgc cccttgtcac gccgacgagc cagttggtgg
      181 gcacccaggc caccctaaac gtactcatgg gagagaggtg gaaaatggtc tccaaggagg
      241 taaagaacta cttcctcggt tactatggga cgcccccggg acctctggac agggaagtcc
      301 agaagaaggt catcggcgac gagaaggcca ttacctgccg gcccggagag gtgctggaac
      361 cggagctgcc aggtgtctac gaagccatgg ggtcatggat catgcagccc gaggatgccc
      421 ttagctacgc tcttttccct gccgtagcaa aggatttcct cgtaaggaag ttcgcccggg
      481 agaatctcag ggacgtaggt ctggaacccc ttgtcgaggg tgcagcctac cctctttagt
      541 gtccgatccc gatcggcggc tgaatgactg gaggtattct tattggagac ggaagaaagg
      601 gccagatccc ttatcaatga acagatcagg ccggcgcttc aggagcatgg aggcgatata
      661 gagatagtgg gatttgatcc cgacggcagc gtacttacag taaggctcaa gggagcatgc
      721 ggcggctgtc cctttgccgc agagaccttg cgaacccagg tggaggccgt catccggaag
      781 gagatacccg agatagaaaa ggtcgttagg atacaatgaa gcatgacagg gcgggaagaa
      841 cagcgggtga cagggttatc tccgacagga gctgttccgt ggaggcgtcc aacgaagaga
      901 ccctttcgcg tcttctcggt cccagggacg agaacctgag gctcatagag gagcgttacc
      961 ctgtaaggct cgccgtcaag gaccttctgg tctccgtaca agggcctgat accgaggtcg
     1021 tcgacctggt ctcggaactg atcaggcagc ttatgggtat tgccagaaag gggcacgtcc
     1081 ttcgacctgc cgaggtcacc tatgccatga acgttttcgc agaggagggt gtcatcgatc
     1141 ttctgcccat gtacgaggac gtaatttgcc tgacctacag gggaaagccg gtacgtccga
     1201 agacaggggg ccagaagcgt tatgttgacg ccataagaag gaacgatgtc ctcttcgctg
     1261 taggtcctgc cgggacgggc aagacctacc tcgccgttgc tctcgccgtg gctgccctca
     1321 aggagggcaa ggtcaacagg gtcgttctcg tccgtcccgc cgtagaggcc ggagagagcc
     1381 tggggtatct tccaggggat ctgaaggaga aggtcgagcc ttacctgagg cccctttatg
     1441 atgccttcta cgaaatgttc tctccggaga ggttcatgaa gtacgtagag aagaaggtga
     1501 tcgagatagc ccccctcgcg tatatgaggg gtaggacatt gaatgacagc ttcattattc
     1561 ttgatgaggc ccagaacacc actcctgaac agatgaagat gttcctcacc agactgggtt
     1621 tcggctccaa ggcggtcgtc ataggggata taacccagat agacctcccc tcgggcaagg
     1681 actctggcct aaaagtggta aggaacatcc tgaagaggat ccccggggtc gaattcgtgt
     1741 ctctcgacaa gaacgacgtt gttcgtcacg aagtcgttca aaggatcgtc caggcttatg
     1801 aagaatatga gcagaaacgg cagtaaagga cccgctctct cccgaatagg gctatacgcc
     1861 gaaaagatgg ccagtgacag ggtataccgt ctgcgctgga cggccacttt cctgctcgcg
     1921 gctgttgcct cctcactact tgcaggcacg tggtgggtga gggatggcag gagttccttc
     1981 cggatcggca gccccgctcc acagaccttc tatgcccatt cacggatgag gatcgtcgac
     2041 gagaacgcca ccagggcgct cagggaacaa aggatgtccg acgtcggtgg tgtccttgtg
     2101 agggacagga ggttcccgga agaggtaaaa agcaaactgg cttcgataga gagtggggag
     2161 tacaggactg ttctgccgga atcactggct ggtatcctgg agagtctccc agagatctcg
     2221 gcagggagga tcgcaaccgt ttcttcaagg gtcgccatat caatgctcag cttcccgatg
     2281 atggaaggat ccccggcaga gacgatctgg aagggccttg aggcctcgga cctatccata
     2341 cccgagcaga acctggcctt ccagatcctt gatcatataa tggagagagc catggtaatg
     2401 gaccctgaaa tgaccagtga ggtcagggtt cttgccgctt cggagataga acctgtggag
     2461 agggtccttg cggtgggaga tgtcatcgtc agggagggtg agacggtatc ggccgaactg
     2521 gctcttgtac tccgggccca gggatacccg gagctatcat tcccctggaa gaccctgacc
     2581 ttcgtcgtcc ttggggtcat tctctgggca tcgtggatgt ggtggtactc cgtgcgccgg
     2641 gagttcaatt tcgactccct ggagtggggc ttcattatca ccatcctgat agccggatgg
     2701 gtcgccatgt tcgtctcggc acttcttgga gagaacggac tcggtgtgat atccctggca
     2761 gggtgggcct accttggcct ttccggctcc ttcgcatttc acctggtcct ttggggtgga
     2821 ataatcggat cggtgataac ttcaggcagt tcaacggtgg gccttatgct ctccgtgttc
     2881 gcttctggga taactgcgag cctcggatac atactcatga ggaagatcac gagccgcctg
     2941 gaccttgtcc ggaagatgtt cctcctgggg ttcttccttg ccttcggggc cttcttcata
     3001 ggttggggcc tatacctgcc atcggacaga tcgatcctag tcacccagat cgtctcggct
     3061 tttgcctggg taatggttgc cctggtcgct cttcctctct gggagagatt cttcgatatc
     3121 ctgaccccaa tccgtctcgt tgaccttaca cacccatctc accccctgct gaaaaggctc
     3181 cagatagaag ccccgggaac ctatcaccac tcgctgatgg tcgggaccct cgccgaagcc
     3241 gcagccggta agcttaagat gaaccctctt ctcgtcaagg caggagcatc tttccacgac
     3301 gtggggaagc tcaggcgacc ccagttcttc gtggagaacc agcagaccgg tcacaatcct
     3361 catgacgaac ttgcgcccac cctttccgcg ctaataataa tctcccatgt cagggagggg
     3421 ctggaaacgg cccggcagtt cgggatcccc aagaagatca gggtcttcat caaggagcat
     3481 catgggacca cctgtctcca ttatttttac aagaaggccc tgagcctgga tccggacctg
     3541 cccatggacc agttctgtta tcccggtgac cgtcccagga gcc
//
LOCUS       DAOPKQ010000145         3507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_29847_length_3507_cov_4.298667, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000145 DAOPKQ010000000
VERSION     DAOPKQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29847_length_3507_cov_4.298667"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PK146_05595"
     CDS             <1..298
                     /locus_tag="PK146_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91750.1"
                     /translation="WMRARVGGISYTRDRFVEEVNRLPGWKAFPSSANFVLLHSLNPK
                     ELILKTFRYRGVHARFPAMGSLGEISGAWVRVGIGLEEEMGRVLEALKDLSTGA"
     gene            348..1706
                     /locus_tag="PK146_05600"
     CDS             348..1706
                     /locus_tag="PK146_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPE91751.1"
                     /translation="MIEIPLDPGGVVPLYRQIATHLIRMVQNGTLALNDRLPGSRDLA
                     TSLGLSRNTVVLAYDLLEDEGYIEQKGRRGAFVSWKRPHKAMGLEDSGPVWDLASGLP
                     SFDMVPTERLASLARDSLLSCGPAALYDTPPEGLMSLRKALVRHAARRGIPAKRDEAV
                     VTSGARHALSVYIHAMALKGVRRIWVEELTYPEVRRMAEGAGLEISAVPVDPDAFLGS
                     LSRILPGDLLYLIPSFHNPTGRTLSYEQRKVVLEAASKRGFWIIEDDAYGELRFGETS
                     VPALKSIDREDRVLYLGSFSQLLFPGMRIGYVLLPEDTRKEFLSVLERTSGSTSSLVQ
                     YVINAFIEDRSLEISLDLARNCMASRMKALCGSISGSMPQLPFETPQGGIYLWLRTPG
                     ISGNLAADTARRADVDMVPGRSFSFPQRDIQAVRLSVSRVSTAMIHEAVGRIASAWKE
                     LP"
     gene            1703..2215
                     /locus_tag="PK146_05605"
     CDS             1703..2215
                     /locus_tag="PK146_05605"
                     /inference="COORDINATES: protein motif:HMM:NF015742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HPE91752.1"
                     /translation="MKGLERLLSGRDERARLQKHLLSHLDVVVQVSLNIPGLPKKMTG
                     DRDLVSLVSDRICLELTLTGSRPRTLFFLDNGAGFAGMTGLKEADPALVKRIGMRLED
                     QEWGPVVDVDVITRKGAIHRKSLTGGERLCLLCGMPSKDCARSRRHEFTDLRSAFQKL
                     LAKALEELGG"
     gene            complement(2224..2442)
                     /locus_tag="PK146_05610"
     CDS             complement(2224..2442)
                     /locus_tag="PK146_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91753.1"
                     /translation="MRLVVLLLSLGTLVLYGRAIAGLWGHMTGHPGPGMIAYGLIGGT
                     VSAVLALLLWRKRIKELDWVSEEEDRQP"
     gene            2568..3221
                     /locus_tag="PK146_05615"
     CDS             2568..3221
                     /locus_tag="PK146_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPE91754.1"
                     /translation="MIMEPRLYTTSADYVYQELRHRIITKQLKPGQRLPEVNIAVQMG
                     VSRTPVREALRRLASEGLVLIIPNSGARLASPTRREMEDTYAVREQLECISVAIAATR
                     ISERNLRRLEDAILEEEKAFEEKNLELYLEVNENFHRIIAEASGNRVLAEFVENILAR
                     TNAYVVFYDPFYDIEDNPSISQHKAIAQALREREREKAVDLMREHLKLSLYDLNKPS"
BASE COUNT          805 a          968 c         1016 g          718 t
ORIGIN      
        1 gtggatgagg gcgagagttg gcgggatatc ctatacccgc gaccgttttg tggaggaggt
       61 caaccggctc cctggctgga aggccttccc cagcagcgcg aactttgttc tccttcattc
      121 gcttaatccc aaggaactta tcctcaagac cttcaggtac aggggtgtcc atgcccgttt
      181 ccctgcaatg ggctctctcg gcgagatatc cggtgcatgg gtcagggtag gcataggtct
      241 tgaagaggag atggggcgcg ttctggaggc tttgaaagac ctctcgaccg gagcctgatc
      301 agctggtgta tcattgatcc agtgaaggaa agaaagggga tgggcccatg atagagatcc
      361 ccctggaccc cggtggagtg gtccctcttt acaggcagat cgccactcat ttgatcagaa
      421 tggtccagaa cggcaccctt gccctgaacg accgcctgcc cggcagccgt gacctggcaa
      481 ccagccttgg cctgagcagg aatactgttg ttcttgccta cgacctcctc gaggacgaag
      541 gttacatcga gcaaaagggg cgacgggggg ccttcgtgtc atggaagagg ccccacaagg
      601 caatgggcct ggaggacagc ggacccgtct gggacctggc ctcgggtctc ccttctttcg
      661 acatggtacc aacggaacgc cttgcctccc tcgccaggga ttcccttctc tcctgcggtc
      721 ctgcagctct ttacgacacc ccgccggagg gactcatgtc ccttagaaag gcacttgtaa
      781 gacatgccgc aaggagaggt attcctgcaa agagagacga ggccgtagtt accagtggcg
      841 cccgccacgc cctttccgtc tacattcacg ccatggcact gaagggggta agaaggatct
      901 gggtggagga gcttacctac ccggaggtga ggcgtatggc cgaaggcgca ggcctggaga
      961 tctcagcggt tcctgtcgat cccgatgcat tcctgggatc actttcccgg atactcccgg
     1021 gggacctgct ttacctgatc cccagcttcc acaaccccac gggccggacc ctttcctatg
     1081 aacagagaaa ggttgtcctg gaggcagcat cgaaaagggg attctggata atagaggacg
     1141 acgcttacgg cgagctccgt ttcggagaga ccagtgtccc tgctctcaag tccattgacc
     1201 gtgaggaccg ggtcctctat cttgggtcct tcagtcaact cctcttcccc ggaatgagga
     1261 tcggatatgt tcttcttccc gaggacacca ggaaggaatt tctgtccgtc ctcgagagga
     1321 catctggttc gacctcatcg ctggtccagt atgtgataaa cgccttcatt gaggacagaa
     1381 gcctggagat ctcccttgat ctggccagga actgcatggc atcccgcatg aaagcacttt
     1441 gcggatccat atctggatca atgccgcagc tgcccttcga gaccccccag ggggggatct
     1501 acctgtggct caggacccct gggatctccg gtaaccttgc cgccgatact gcaagaagag
     1561 ctgatgtaga catggtgccc ggaaggtcct tcagtttccc ccagagggat atccaggccg
     1621 tgagactctc cgtgagcagg gtttcaacgg cgatgatcca cgaggcagtg ggacgtatcg
     1681 cctctgcctg gaaggaactg ccgtgaaggg gctggaaagg cttctctcag ggagggatga
     1741 gagggcccgg ctccagaagc atcttctcag ccacttggat gtagtggttc aggtgtcgct
     1801 gaatatacca gggcttccaa agaaaatgac cggcgacagg gatctggtat ctctcgtatc
     1861 ggaccggata tgccttgaat tgaccctgac cgggtcaagg ccaaggaccc tcttctttct
     1921 cgacaacgga gcaggctttg ccggaatgac ggggttaaag gaggccgatc cggccctggt
     1981 caaaaggata ggaatgcggc tggaagacca ggaatggggt ccagtggtag atgtggacgt
     2041 tataacccga aagggcgcga tccacaggaa aagccttaca ggaggggaga ggctttgcct
     2101 tctctgcggg atgccttcca aggattgcgc cagatcgcga cgtcatgaat tcaccgacct
     2161 tcgcagcgcc ttccagaagc tccttgcaaa ggccctggaa gagctcggcg gctgaaggga
     2221 ctttcagggc tgcctgtctt cttcctcgct gacccagtcc agctccttta tgcgctttct
     2281 ccaaagcagt agcgccagga ccgctgaaac agtcccgccg atcagcccgt aggcgatcat
     2341 tcccgggccg gggtgacctg tcatatggcc ccagagcccg gctatcgccc ttccgtagag
     2401 gaccagtgtc cccaaagaca acagtagaac tacaagtcgc atggcctgca ccccgatccc
     2461 tggattgctt tgaattataa cctgaaaaat tgttcgttcc tgttatttgt gatagcatgc
     2521 atgtattcat gtaataccat ttggctttga agagagggga gatccacatg atcatggagc
     2581 ccagactata caccacttca gccgactatg tttaccagga gctgaggcac cggataatta
     2641 ccaagcagct caagccggga cagcgtcttc ctgaggtcaa cattgcggtc cagatgggcg
     2701 tgagccgcac ccctgtaagg gaggccttga gaaggctggc ctcggagggg ctggtgctga
     2761 taatcccaaa cagcggagcc aggcttgcct cacctaccag aagggagatg gaggatacct
     2821 acgccgtcag ggagcagctg gagtgcatct cggtggctat cgctgccacc cggataagcg
     2881 aaaggaacct caggaggctc gaagacgcaa tcctcgaaga ggagaaggcc tttgaggaga
     2941 agaatctcga gctgtacctc gaggtcaacg aaaactttca caggatcatt gccgaggcca
     3001 gcggaaaccg ggtcctggcg gagttcgtcg agaacatcct tgcgaggacc aacgcctacg
     3061 ttgtattcta cgatccattc tacgacattg aggacaatcc cagcatatcc cagcacaagg
     3121 cgatcgccca ggccctcagg gagagggaaa gggaaaaggc cgtggatctc atgagggaac
     3181 atctgaagct ctctctttac gatctcaaca agccctcctg accccgaaag aggtcatacg
     3241 gagcttccac ttgctccccc gacagtgtga gaatagtagg tgtacaagac ttaaacactt
     3301 cacgcaagat cagaaatgtt cctggaaagg ggagcgccat atgagcgaga aaatgctgcc
     3361 atccttcgat ctcacaagga actgggagcg agtcagggag gagaccctga ctgcgatcag
     3421 aaaagtcctg gagagccagc agttcatcct gggacccgag gtggaggccc ttgaaaagga
     3481 tctagcgtac tacctgggaa cactcca
//
LOCUS       DAOPKQ010000146         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_30106_length_3487_cov_4.366550, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000146 DAOPKQ010000000
VERSION     DAOPKQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30106_length_3487_cov_4.366550"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PK146_05620"
     CDS             <1..966
                     /locus_tag="PK146_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPE91755.1"
                     /translation="PRAGLILSVVVLSLLLAICLLWVISVGEVPIPRGDVLGVISGRI
                     PSSDPRSVIVLSIRLPRALAAAAAGAVLSGSGVIFQGVLLNPLAEPYTLGVATGAALG
                     ASLAITLGLPFVSAFAFIGGIGALWLVWILGRGAGETASPTRLILAGVIVGSILGAGI
                     TLIKALAGDQVGIIVLWLLGSFSLSSWEHVAWGAASALAILVLGLFWARDLDIMSSGA
                     DPRSLGVDDRKTSGFLLLGSSLATAVVVSMSGVIGFVGLVVPHLVRIVSGPAHFRLLP
                     LSILGGALLLLLADTAARSLGELPVGVITALIGGPIFCFLLWRER"
     gene            966..1733
                     /locus_tag="PK146_05625"
     CDS             966..1733
                     /locus_tag="PK146_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91756.1"
                     /translation="MSLLSLKGVRVTLGSREVLKGIDLQVMPGEVVALLGPNGSGKST
                     LLAAAAGVGRRDTGRVLVKGDDLDSLSRREVARRIAVVPQQTGFMMPFSVMETVLMGR
                     FPLAGRFARLSAGDRLVAAEALEAVDLGSFGERLVTDLSGGEAQRVAIARALAQQTDI
                     LFLDEPVSALDPRHAIAVFRLLEVLRKEGKGILVSLHDINYALRFADRIVFLKDGLLV
                     EPPSPRKVDEVLLAEIFDTPWILEELSGGFRAAFPLV"
     gene            complement(1742..>3487)
                     /locus_tag="PK146_05630"
     CDS             complement(1742..>3487)
                     /locus_tag="PK146_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPE91757.1"
                     /translation="TPEGRSVEDRKVLLVGNPNVGKSALFTRLTGVHAFSSNYPGTTV
                     GFTEGYLRFEGVRYRLIDVPGAYTLEPTNEAEEVAKRIIEEGANVVVITVDATALERN
                     LNLALQVLEMGHPSVVALNMVDEARHEGVNIDIPALERSLGVPVVGTVAVTGQGISEL
                     ISRLGEARPGIAVPRADRWARIGEITASVQTMEHRHHTIRDRIEDLSVHSGWGALLGV
                     FAVLGSFTAVRIIGEGLIDYLLDPIFNGLFMPVFEWLGPLLGGEGSLLFRTLIGMLID
                     GKIDPELSFGLLTTGLYVPLVMVLPYVVAFYAVLSFLEDVGYLPRFAVLFDALLHKVG
                     LHGFAIVPNLLGLGCNVPGIMATRILDSPRERFIASTLISVAIPCAGLQAMIIGALGD
                     LGGRYVLAVYATLFASWVILGRILHKLLPGYSPELVVEIPPYRMPSFKGIGMKLWFRI
                     RGFLIEAIPMVLAGVFVANLLSTSALMESVSTFLSPLFRGVLGLPPETAGPILLGFLR
                     KDIAVGMLIPLGLQPNQMVVAVVTLAMTFPCIATFIELLRELGLADMLRSIGIMLLAA
                     LTAGGILNALFALVS"
BASE COUNT          635 a         1050 c         1082 g          720 t
ORIGIN      
        1 cccagggcgg ggttgatcct ctccgttgtt gtcctttccc tcctccttgc catctgtctt
       61 ctgtgggtca tctccgtggg cgaggtcccc ataccccggg gtgatgtcct cggggtcata
      121 tcgggtcgta tcccttcatc ggaccccaga tcggtgatag tcctctccat acgcctgccc
      181 agggcactgg cggccgcagc tgccggagct gttctttcag gcagcggtgt gatctttcag
      241 ggtgtccttt taaatcccct tgcggaaccc tacaccctgg gtgtggccac cggcgctgct
      301 ctcggtgcct ccttagcgat cacgcttggc ctccccttcg tctcggcctt cgccttcatc
      361 gggggcattg gcgccctctg gctcgtctgg atccttggga ggggggccgg tgaaacagca
      421 tccccgaccc gcctgatcct ggccggtgtc atagtgggca gcatactcgg tgctggtatc
      481 acccttatca aggcactggc aggcgaccag gtggggatca ttgtcctctg gcttctgggg
      541 agtttttctc tttcctcgtg ggaacatgta gcctggggtg ccgcctcggc cctggcgatc
      601 ctggtcctgg gccttttctg ggcccgtgac cttgacatca tgtcctccgg agccgacccc
      661 aggtcccttg gtgtggacga caggaagacc tcgggctttc ttctcctggg cagctccctt
      721 gctacggcag ttgtggtatc catgagcggc gtcatcggtt tcgtaggcct agtggtccct
      781 cacctggtga ggatagtgtc aggcccggcg catttcaggc tccttccgtt gagcattctg
      841 gggggtgcct tgctcctgct gcttgctgac accgctgccc gctctctggg ggagcttccg
      901 gtgggggtaa ttaccgccct catcggagga ccgatcttct gcttcctgct ctggagggaa
      961 agataatgag ccttctgtca cttaaggggg tcagggtcac tctcggttca agggaggtcc
     1021 ttaaagggat cgaccttcag gtaatgcccg gggaggtcgt agcgctcctc ggccccaacg
     1081 ggagcggcaa gagcaccctc ctggcggccg cagcgggcgt aggcagaagg gataccggcc
     1141 gggtccttgt caagggagac gatctggact ccctttccag gcgggaggtc gccagaagga
     1201 tagccgttgt gccgcagcag acggggttca tgatgccctt ctctgtcatg gagacggttc
     1261 taatggggcg cttccccctg gcaggcaggt ttgccaggct gtccgccggg gaccgcctgg
     1321 tggcggccga agcgctggag gcagtcgacc tgggatcctt cggtgagagg ctggtgacgg
     1381 acctgtcggg cggggaggcc cagagagtgg ccatagcaag agccctcgcc cagcagacgg
     1441 atattctttt cctggacgaa cctgtcagcg ccctcgaccc gcggcacgcc atcgccgttt
     1501 tccgtcttct cgaggtgttg cggaaggagg gaaagggcat ccttgtgtcc ctccacgaca
     1561 taaactacgc tctcaggttc gccgacagga tcgtattcct caaagacggc ctcctcgtag
     1621 agccgccgtc gccaaggaag gtggatgaag tgcttcttgc ggagatcttc gacaccccct
     1681 ggattcttga agagctctcc gggggcttca gggccgcttt cccgctggtc tgagaggacc
     1741 tctaggagac aagggcgaag agggcgttaa gaatccctcc ggcggtcagt gccgccagaa
     1801 gcattatccc gatggaacgc agcatgtccg ctagtcccag ttcacgcaac agttcgataa
     1861 aggtggctat gcaggggaag gtcatggcca gggtgaccac ggctactacc atctggttgg
     1921 gctgcagccc caagggaatg agcatcccta cggcaatatc ctttctcaaa aaacccagca
     1981 ggatcggtcc ggccgtctcg ggcgggaggc ccagtacgcc cctgaagagc ggggagagga
     2041 aggtggatac gctttccatc aaagccgagg tggagagcag gttggccacg aaaactcccg
     2101 ccagcaccat tgggatggcc tcgataagga atcccctgat cctgaaccag agcttcatgc
     2161 cgatcccctt gaatgaaggc atcctgtagg gcggtatctc caccaccagt tcggggctgt
     2221 aacccgggag aagcttgtgc agtatcctgc ccaggatgac ccaggaagcg aaaagcgtag
     2281 catatacggc cagaacgtac cgccccccca ggtcgccgag ggctcctatg atcatggcct
     2341 gaagccccgc gcagggtatg gccacggaga tcagtgtgga ggcgatgaag cgctccctgg
     2401 gtgagtcaag gatcctggtc gccatgatac ccggtacatt gcaccccagg cccaggagat
     2461 tggggactat ggcgaagccg tggaggccca ccttgtggag aagcgcgtcg aagagcactg
     2521 cgaatcgggg gaggtagcct acgtcctcaa ggaagctgag taccgcgtag aaggccacaa
     2581 cgtagggaag caccatcacc agcgggacgt agagccccgt ggtcagaagg ccgaaactga
     2641 gttccgggtc gatcttcccg tcgatgagca ttccgataag agtcctgaac agtaaggagc
     2701 cctcccctcc gagaaggggt cccagccatt cgaagacggg catgaacaga ccgttgaaga
     2761 tcgggtctag caggtaatca ataaggccct cgcctattat tcgcacggcc gtaaagctgc
     2821 ccaggacggc aaaaacaccc agaagagccc cccaccccga atggaccgaa aggtcctcga
     2881 tccggtccct tatggtgtgg tgcctgtgtt ccatggtctg taccgaggcg gtgatctcac
     2941 cgatacgggc ccagcggtca gctctgggca cggctatgcc gggcctggcc tcgccaagac
     3001 gtgatatcag ttcactgatc ccctgccctg ttacggcgac ggtgcccacc acgggcactc
     3061 ccagcgatct ctccagggct ggtatgtcta tgttgacccc ctcgtggcgt gcttcatcga
     3121 ccatgttcag ggccacaaca gaggggtgtc ccatctcgag gacctgcagg gccaggttga
     3181 gatttctctc cagagcggtg gcgtcgacgg ttatcaccac gacgttggcc ccttcctcta
     3241 tgatcctttt ggcgacctcc tcggcctcgt tggtaggctc cagggtgtag gcgcccggga
     3301 catcgatcag gcggtacctc accccctcga aacggaggta cccctcggtg aaaccaacag
     3361 ttgtgccggg gtagttcgag gagaaggcat gaactcctgt cagacgggtg aagagggcgc
     3421 tcttccccac gttggggttg cctaccagga ggaccttcct gtcttcaacg ctcctgccct
     3481 caggggt
//
LOCUS       DAOPKQ010000147         3481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_30188_length_3481_cov_5.840630, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000147 DAOPKQ010000000
VERSION     DAOPKQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30188_length_3481_cov_5.840630"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..1141)
                     /locus_tag="PK146_05635"
     CDS             complement(386..1141)
                     /locus_tag="PK146_05635"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HPE91758.1"
                     /translation="MSLTDLLGAMMQSGMAPSSRKRAKNVLGAESGDLMESLSGLVGG
                     KGGDLGGILGEVLNSAGRAAGGKQNLAVGGLGALAGALLGGGTRSMGGALGGGAMALI
                     GAIAFKALQGAGQKTDNVPLGLLQPRNQAEQQKLEQQTELVLKAMINAAKSDGRISED
                     EIQRIVGKLKEAGAQDDDMRYVMSEMQKPIDTYSLMAAAKGQPELAAELYAASLMAIE
                     VDTTAEKAYLQELAGGMGLSPEVTRQIDQMVGL"
     gene            1246..1596
                     /locus_tag="PK146_05640"
     CDS             1246..1596
                     /locus_tag="PK146_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91759.1"
                     /translation="MSEPEHITNISSTAEDAEIHREIPETRRKAAGEQEGAGSRGESE
                     IRGRRKQFAIIVFLECWIIFSAGFCKEAWIDYENCQGLVDIDIGRHPLFFCPDRLGFT
                     FFDSLQGSLGLQGH"
     gene            1607..3097
                     /locus_tag="PK146_05645"
     CDS             1607..3097
                     /locus_tag="PK146_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91760.1"
                     /translation="MKGIITSPEEGPEGYWHFADRGEAAAALARTLEVFDASTATEEE
                     KSNLKRILVFFAKDLETAGVIFPKDDLLAASAGTEDVQPSRFETAGLLARTLETVDRE
                     TIDKADLERVDRLVDEYREELDLWAPDLEGLEERVAQIEEGVGGWRLSGALRFDANYY
                     RAGAVGSEGYGIYGDEPGQDGDMRFSRMWLNISKRVDDKVSFFSRLEMNPRSKENELF
                     LDQAYFLTDFPGNWSSYIGRWYFDWEADDSLYLGEEPIFTHRVMNGFMIRYDLPMGHF
                     EAYASRPQKETDVEQGYEFGARYKMHLSERLWFSLNVIERQLYEDFDKQVLWAVAGLD
                     LNPFWTVRGAIYRQDTGTDTPYAFQAILQGKQTERGLTSFWLEFMSFDQGFELWNRAY
                     QDYGTFMGWNDGEGGHPTTPVGAVYDNILFIRLDQQWDEKISTFQRYVLALHRYDGEN
                     AINFTVGLKYRYTPHIAFVLVYDKVEEAFDEGLDDHLIQLRTEVWF"
     gene            complement(3228..>3481)
                     /locus_tag="PK146_05650"
     CDS             complement(3228..>3481)
                     /locus_tag="PK146_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPE91761.1"
                     /translation="EGLIKIVTDSGGRNHGATIVGENASELIHEWTMAIQYKKKMHHL
                     MMMQHSFPSISMINKMITENWMMEKMKTPWLQKLIKKLV"
BASE COUNT          795 a          888 c         1016 g          782 t
ORIGIN      
        1 cctgccttgc tccgagaagc atgacaacgg ggctggtctc cgtttccctg atgagagcct
       61 cctctgccca tcgagggatg taactgggtt ttttcatttc gacctccgaa aatattatta
      121 gcgtatggcg ctattttatt acgaaggaag aagtatggca agtttgaagg gtgaaggaag
      181 atctgaagca aggcgtcacg ggagccttgt tcaaagttca aagtttcaag ttcaaagtga
      241 atcgtaaacc caaaggcttt ggggtcagac cttgaattgc agcaattgta cacgtttcca
      301 ggcgactgcg ctgaatagac cagggacatg aaagaaaaaa ggggccctcg tgggcccctt
      361 aggttgggct attggagcag gaacgctaaa ggccgaccat ctggtcgatc tgccgggtaa
      421 cctcgggtga caggcccatt ccgccggcaa gttcctgcaa gtaagctttc tcggccgtgg
      481 tgtccacttc aatggccata agcgaggccg cgtagagttc agcggccagt tcgggctgac
      541 ctttcgcggc tgccatcagg ctgtaggtat ctatcggctt ctgcatttca gacatgacgt
      601 agcgcatgtc gtcgtcctgg gccccggctt ccttgagttt gcccacgatg cgctggatct
      661 cgtcctcgct gatgcggccg tcggacttgg cggcgttgat catggctttc agcacgagtt
      721 ccgtctgctg ttccagtttc tgctgttccg cctggttgcg gggctggagc agtccgaggg
      781 gaacattgtc cgttttctgt cctgcgccct gcaaggcttt gaaggcaatg gcaccgataa
      841 gtgccatggc tcccccgcca agggctccgc ccatggatct ggtaccgccc ccgagaagtg
      901 cgcccgcaag ggcacccagc ccccccactg caaggttctg ctttcctccg gcggcacggc
      961 ccgcggaatt gaggacctct ccaaggatcc cgcccagatc tccccctttg cctcccacca
     1021 ggcctgaaag gctctccatg aggtctccgc tttcggctcc aagaacgttc tttgccctct
     1081 ttctggatga aggggccatc cctgactgca tcattgcacc aagaagatcc gtgaggctca
     1141 ttttagacat cctcctctta aaaattcagt aagttgaagt ctgtttaaga tacgcctgaa
     1201 aggaggggaa ggcaagaccc gttacaggtg acgcgtaact cgaaaatgtc ggagccagag
     1261 catattacga acataagcag cactgcagag gatgcagaga ttcacaggga aatacctgaa
     1321 acaaggcgga aggcagcagg ggagcaggag ggggcaggat caagggggga atcagagatc
     1381 agggggagga ggaaacagtt tgctataatt gtatttttag agtgttggat aattttctct
     1441 gcaggttttt gcaaggaggc ctggatcgac tatgagaact gccagggttt ggttgatatt
     1501 gacattggtc gtcatccttt gttcttctgc ccagatcgcc tggggttcac ctttttcgac
     1561 agtctccagg ggtcactggg cttacaagga cattgagtac cttagattga agggtatcat
     1621 caccagtcct gaggaaggac cggaggggta ttggcacttc gccgacagag gcgaggcggc
     1681 tgcagccctg gctcggactc tggaggtctt tgacgcttcg acggccacgg aagaggaaaa
     1741 gtcaaacctt aaaaggatcc tcgtcttctt cgcaaaagat ctggagacag cgggcgtgat
     1801 ctttcccaaa gatgatctac tggctgcatc agcaggaacg gaagatgttc aaccctcccg
     1861 gttcgagacg gcaggtctcc tggccagaac tcttgaaacc gtcgacaggg agacaattga
     1921 caaggcagac cttgaacggg tcgatcgcct ggtcgatgag taccgtgagg agctggacct
     1981 ctgggctcct gatctggagg gacttgaaga gagggtcgcg cagatcgagg aaggagtcgg
     2041 gggttggcgt ttgagcggcg ccttgcgctt tgatgcgaac tactaccgcg cgggcgctgt
     2101 tggaagcgag ggctacggga tctacggcga tgaacccggc caggacgggg acatgaggtt
     2161 ctcccgaatg tggctcaaca tttcaaaaag ggtggacgac aaggtctcct tcttcagcag
     2221 actggagatg aacccccgtt ctaaggaaaa cgaactcttc ctggatcagg cctattttct
     2281 taccgacttc cccggcaact ggagctccta catagggcgc tggtacttcg actgggaggc
     2341 cgatgacagc ctctatctgg gcgaggagcc catcttcacc catcgagtga tgaacggctt
     2401 catgatcagg tacgaccttc ccatgggcca ctttgaggcc tatgcctcga gaccgcagaa
     2461 ggaaacggat gtagagcagg gctacgagtt cggcgcacgg tacaagatgc acctttcgga
     2521 aaggttatgg ttctcgttga acgtaataga gcgccagctc tatgaagact tcgacaagca
     2581 ggtcctttgg gctgtcgcgg gactggatct taatcccttc tggacggtca ggggagccat
     2641 ctacaggcag gacaccggaa cggacacccc ctacgccttc caggccattc ttcaagggaa
     2701 acagaccgag agaggcctga catccttctg gcttgaattc atgagctttg accagggctt
     2761 tgaattatgg aacagggcct accaggacta cgggaccttc atgggctgga acgacgggga
     2821 ggggggacac cccaccacac ccgtgggtgc agtctacgac aacattcttt tcataaggct
     2881 ggaccagcag tgggatgaga agatttccac cttccagagg tacgttctcg ccctgcacag
     2941 gtacgacggc gaaaatgcaa tcaatttcac tgtgggtctg aagtaccgtt acacacccca
     3001 catcgccttc gtccttgtct acgacaaggt ggaagaggct ttcgacgagg gactggacga
     3061 tcatctgatc cagctaagga cagaggtgtg gttttagggg acaaaggcgt gaacggggag
     3121 cgggcgcaaa agcgaggcgt caggaaaaac caggatccgg gagacgttag acgaaaaggc
     3181 aaggtctaaa agagcgtcaa cggtaaacaa taaaactgaa aactgatcta taccagcttc
     3241 tttatcagtt tctggagcca gggggtcttc attttttcca tcatccagtt ctcggtgatc
     3301 atcttgttga tcatggagat ggacgggaac gagtgctgca tcatcatcag gtggtgcatc
     3361 ttctttttgt actggatggc catggtccat tcgtggatga gttcgctggc gttctccccc
     3421 acgatggtgg ccccgtggtt gcgccccccg gagtcggtga cgatcttgat taaaccctcg
     3481 g
//
LOCUS       DAOPKQ010000148         3481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_30190_length_3481_cov_4.652072, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000148 DAOPKQ010000000
VERSION     DAOPKQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30190_length_3481_cov_4.652072"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK146_05655"
     CDS             complement(<1..273)
                     /locus_tag="PK146_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91762.1"
                     /translation="MPQWIEVRGARQHNLKNIDVDIPKNCLVVITGPSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGVQDKPDVDDISGLSPAISIEQKGV"
     gene            complement(203..2242)
                     /gene="uvrB"
                     /locus_tag="PK146_05660"
     CDS             complement(203..2242)
                     /gene="uvrB"
                     /locus_tag="PK146_05660"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPE91763.1"
                     /translation="MSDRTSGFKLFSEWPPAGDQPEAIDRLVEGFRSGERFQCLLGVT
                     GSGKTFAMANVIERLQRPALVLAHNKTLAAQLYSEFKEFFPGNAVHYFVSYYDYYQPE
                     AYVPVSDVYIEKDASINDRIEKLRLATTKSLLERRDVIVVASVSCIYGLGRRKAYEDA
                     IFRFSLGERYERRDFLSRLVTNHYERNDHDLQPGTFRARGGVIEVFPAYSDTAVRVHM
                     EDDLIERIDEIEPLTGRSILSKPYAAIFPAQHYITSREAIARALPMIEEELQERLEHF
                     RSRGKLLEAQRLEMRTRYDMEMLTQVGYCSGIENYSRILDGREPGETPGTLLDFFPED
                     LLFFIDESHITIPQVQGMFNGDRARKQTLVDHGFRLPSCLDNRPLRWEEFCSFLRQTC
                     FVSATPGDWETSVSTRVAELIARPTGVVDPEVLVVPATGQIDDLTERLRKVVARGERA
                     LVTTLTKRSSEDLAEYLSEIGFGVKYIHSELDAFERTELIRDLRKGEISVLVGVNLLR
                     EGIDLPEVSFVGILDADREGFLRSRRSLIQIIGRAARNTAGQVILYADQETGSIRSAM
                     EETTRRREKQLNFNRVHNITPKTIHKKIMPLLPEEEIADIAAGISVRSGGEGFTSQEL
                     ERMMWKAVEDLDFERAALIRDILQGKRGGGSIRAAMDRGKRGKTAQPEKHRRRHT"
     gene            complement(2365..>3481)
                     /locus_tag="PK146_05665"
     CDS             complement(2365..>3481)
                     /locus_tag="PK146_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE91764.1"
                     /translation="RVLLRDRHRGAGLGGGHDEREQTLNQLLVEMDGFDVSSGIILIA
                     ATNRPDILDPALLRPGRFDRHIVVDRPDRNGREAILKVHLREMKVEEDVDLEVLAKRT
                     PGFVGADLANLVNEAALLAARRERKNISMAEFEESIDRVLAGPERRSRLISDREKEII
                     AFHEVGHALVAKMVPGCDPVHKISIIPRGHKALGYTLQLPEEDRFLMSKKELTNRICV
                     LLGGRVAEEIHFSDITTGAQNDLERATQIARQMVTEFGMSDTLGPVTLGRKQHEVFLG
                     RDIMEDRNYSDEVAYAIDQEVRSIVESCYERVRNILKENQEAHVRIARTLLVEEVIEG
                     DRLDSLLEEGIVGSPEAAEEAVATVGEIEDEEGGSVA"
BASE COUNT          695 a         1068 c          882 g          836 t
ORIGIN      
        1 gacgcccttc tgttcaatgg aaatggctgg agagagaccc gaaatatcgt ccacgtcggg
       61 tttgtcctgt actcccagga actgtcgggc gtaggaggac agggattcga cgtagcgtct
      121 ctgcccctcg gcatagatag tatcgaaagc cagcgaggat ttccccgatc cggagggccc
      181 agtgatgacc acaaggcagt tcttaggtat gtctacgtct atgtttttca ggttgtgctg
      241 tcttgcccct cttacctcta tccattgcgg cacggatgga tccgcctccc cgttttccct
      301 gaaggatgtc ccttataagc gcagctctct cgaagtccag gtcctccacg gccttccaca
      361 tcattctctc aagctcctgg ctggtgaaac cctctcctcc ggacctgacc gaaatacctg
      421 cagcgatatc ggcgatctcc tcctccggca gaaggggcat gatcttcttg tgtatggtct
      481 tcggagtgat attatgcacc ctgttgaaat tcaactgttt ttcccttctc ctggtcgtct
      541 cttccatggc agaccttatg ctccccgtct cctggtcagc gtagaggata acctgccctg
      601 ccgtgttccg ggcagctctc ccgatgatct ggataaggga ccttctggac cggaggaatc
      661 cctccctgtc agcatccaga atgcccacga aggagacctc cgggagatct attccctccc
      721 tgaggaggtt cactcccaca aggaccgata tctccccctt gcgaaggtcc ctgataagct
      781 ccgtcctttc gaaggcgtca agctccgaat ggatgtattt gacaccaaat ccgatctctg
      841 aaaggtattc ggccagatcc tccgaggacc tttttgtcag ggtggtcaca agagctctct
      901 cgcccctggc caccaccttc cttagcctct ctgtcagatc gtctatctgc ccggtggccg
      961 gcaccacaag cacctccggg tccactactc cagttggacg cgctatcagc tcggccactc
     1021 tcgtggagac agatgtctcc cagtccccgg gggtggccga tacgaagcag gtttgtctga
     1081 gaaaggaaca aaactcttcc cacctgagcg ggcggttatc aaggcatgag ggcagtctga
     1141 aaccatggtc caccagggtc tgctttctcg ccctgtcgcc gttgaacatt ccctgcacct
     1201 gggggatagt gatatgtgac tcgtcaatga aaaagagaag gtcctcgggg aagaaatcca
     1261 gaagcgttcc cggggtctca ccgggttctc gtccgtcgag tatcctggaa tagttctcta
     1321 tccccgaaca gtaccccacc tgggtcagca tctccatgtc gtaccttgtc ctcatctcca
     1381 gccgctgtgc ttcgagcagt ttaccccggg acctgaagtg ctccagcctt tcctgaagct
     1441 cctcctcgat catgggaagt gcccttgcta tagcctcacg ggaagtaatg tagtgctgtg
     1501 cagggaagat agccgcataa ggcttggaga ggatacttct gcctgtaagc ggttctatct
     1561 cgtcgatcct ttctatcagg tcatcctcca tgtggaccct caccgccgtg tcactgtagg
     1621 cggggaaaac ctctattacc ccgccccggg cccggaaagt ccctggctga agatcgtgat
     1681 cattcctctc gtagtggttg gtgaccagtc ttgagaggaa atcccttcgc tcataccttt
     1741 caccgaggga gaagcggaag atggcatcct cgtaggcctt cctccttccc aggccgtaga
     1801 tgcatgaaac acttgcaacg acaatgacgt ccctcctctc gagaagggat ttggtggtgg
     1861 caagcctgag cttttctatc ctgtcgttaa tggaggcgtc cttctctatg tatacgtccg
     1921 agacggggac ataggcctcg ggctggtagt aatcgtaata gctgacgaaa tagtgaactg
     1981 cgttaccagg aaaaaactcc ttgaactcac tgtacagctg ggctgccagg gtcttgttgt
     2041 gagcaaggac aagggccggt ctctgcaacc tttcgatcac gttcgccatg gcaaaggtct
     2101 ttccgctccc tgtcacacca agcaggcact ggaaacgctc cccgctcctg aaaccttcga
     2161 caagacggtc gatagcttca ggctgatccc ctgctggcgg ccattcagaa aaaagcttga
     2221 aacctgaagt tctatcgctc aaggcaccct ccatcttcca gagtaataaa gagaaaagag
     2281 cgtccggcct caagggatca gaccgaacgc ccgttacatc cagatgccga agcggccgtt
     2341 cctttcaggg tcgtcacaac gccctcaggc tacggaccct ccctcttcgt cttcgatctc
     2401 cccgactgtg gcaactgctt cttccgctgc ttccggggac ccgactatac cctcctcgag
     2461 gagagaatca agcctgtcac cctctatcac ctcttcgacc agcaaggtcc ttgcgatcct
     2521 gacatgagcc tcctgattct ccttgaggat gttgcggacc ctctcgtaac agctttcgac
     2581 tatactcctt acctcctggt caatagcgta ggccacctca tcgctatagt tcctgtcctc
     2641 catgatgtcc cttcccagga agacctcgtg ctgcttcctt ccaagagtga cggggccaag
     2701 ggtatcgctc atcccgaact ctgtcaccat ctgcctggcg atctgggtgg ctctttccag
     2761 gtcgttctgg gctccggtgg taatgtcaga aaaatggatc tcctctgcta cccttccgcc
     2821 caacagtacg catatcctgt tggtaagttc cttcttggac atcaggaaac gatcctcctc
     2881 ggggagctgc agagtgtagc ccagggcttt gtgtcccctg ggaatgatgg agatcttgtg
     2941 gacgggatcg cacccgggta ccatcttggc aacaagggcg tgtcccactt catggaatgc
     3001 gatgatctcc ttctccctat cgcttataag gcgggacctt cgctccggtc ccgccaggac
     3061 cctgtcaata gattcctcga actctgccat ggagatgttc ttcctttctc tcctagcggc
     3121 gagcagggca gcttcgttca ccaggttggc caggtcagcc ccgacaaacc cgggggtcct
     3181 cttggccaga acctcgagat caacgtcctc ctccaccttc atctccctca ggtggacctt
     3241 gaggatcgcc tctcgtccgt tcctgtccgg gcggtcaacc actatgtgac ggtcgaacct
     3301 ccctggccgg agaagagccg ggtcgaggat atcagggcgg ttggtcgcgg cgataagtat
     3361 gatccccgat gacacgtcga aaccgtccat ctcgaccaga agctggttca gggtctgttc
     3421 cctctcatcg tgtcctcctc cgagaccggc accgcggtgc cggtctcgga ggaggacacg
     3481 a
//
LOCUS       DAOPKQ010000149         3468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_30348_length_3468_cov_4.712277, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000149 DAOPKQ010000000
VERSION     DAOPKQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30348_length_3468_cov_4.712277"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..621
                     /locus_tag="PK146_05670"
                     /pseudo
     CDS             130..621
                     /locus_tag="PK146_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            708..1682
                     /locus_tag="PK146_05675"
     CDS             708..1682
                     /locus_tag="PK146_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91765.1"
                     /translation="MRNTRPPYRILVTGTRGKSTAVRYIAAALDSIGLSFSARITGTV
                     PMVLEGLHEKRIIRHSPAHLSEMTWWIRSIPEGTQAVVAENSAVSPELQPLPSKWLDP
                     GLIVWTSLLPDHADCWGPQLSGAREALLRGVPGNSRLLLGNQAGQDLELVLALKKMGC
                     DLFSVGQTKEELPRQYERIAVEALNILGLPGSEASFQKVPRDPHEFMIVNPRRGGAVA
                     WAFSSNDPVTADALFRSTGWDQERTALLFNHRHDRPSRLSAHLPWIRNGKWKRRIITG
                     DRPLIPTGFSFFPHSGAAALADLVRSEGSVFGCGNVRGLPLAREVEAS"
     gene            1679..2095
                     /locus_tag="PK146_05680"
     CDS             1679..2095
                     /locus_tag="PK146_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HPE91766.1"
                     /translation="MNREILVIAIGIALGMLFFKRTGLSPGGIISPGILALHMNTFHA
                     FAWTLAFSLLLFFLLEISVRLFGVYGRQRIALSLLLAALTALLALGRLPLDPLWLGWV
                     VPGLVASDIQRQGLLPTLSALLSLAGVTFLAGGLLP"
     gene            2092..3120
                     /gene="pgsW"
                     /locus_tag="PK146_05685"
     CDS             2092..3120
                     /gene="pgsW"
                     /locus_tag="PK146_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPE91767.1"
                     /translation="MKGVFSILKKGANRQTATIALWALLVAALAWLPGDSPLSQEESR
                     LLQRIEAAWDSLLVLRSETGVPHSETDDPQRSGMIGVEWSTITTTSGSLASKQLSVRP
                     AWAVVFRRWLAREGLGPGDKVTILSSGSFPGLAVSSLSAAESLDLDVTLVISLGSSTW
                     GANIPSMTICDILHFLRTRGFVRTRAAACTIGGAGEMGKDLPPEGLSALEEAARRGFI
                     PLIAAKDLEEMIERKAAVSLPEGTRLVIQIGGSNADLGSDPSLLDLPPGFLRPSPGLK
                     AGNGVVSKALERGITVLHILNIPELARSAGLEGRSSLPWERSPWRFLVAAASGMIVLS
                     FFRRWEFK"
     gene            3117..>3468
                     /locus_tag="PK146_05690"
     CDS             3117..>3468
                     /locus_tag="PK146_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91768.1"
                     /translation="MRTPGERTIETSGGKSSQALWGTILLAAWGVSHFYERPIYHVFR
                     FLDLSVRHDDSGYLLAATAVLVVINAARALLLYEGWFLVGNGVAEATGHRSVGRILPL
                     VGIPVCYQAVAVFDF"
BASE COUNT          677 a         1051 c          971 g          769 t
ORIGIN      
        1 gccgtctgtt acatataccc aacccttgag aatataaatt ccctttttaa gggtatagga
       61 actttttatt tcaaactcct gatctccact accaatttga ttgccattaa ataaaacacc
      121 gtcaactact tcttcggatc aggggtcttc gctaccgagt acgggttcgt cctgaacaac
      181 gagatggacg acttctccca gaaccccgag agtgtaaatg cccccgagcc cggcaaacgc
      241 ccgctctctt cgatgtcccc atcgatcgtc ctcgaccccg acggacaacc cttcatgacc
      301 ctcggggcag caggggcaat gaggatcatc accgctatga cccagataat aatgaacgtc
      361 gtcgacttcg gcatgcacat ggatgaggct atcgaacagc cccggatctt cgctttctcc
      421 tcgggcggcc agagcggtcc tcttcgcgtc gaggacgcaa tgaacgagat caccatccag
      481 ttccttaaac tcaggggaca caacgtggag gtccagccca gggggggcta ttacggaacg
      541 gcccagggga tcctctttat cgacggaatg atgaacggtg gcgccgacag cagacgcctg
      601 ggggtacctg tgggattcta gggcaaaaaa ccgccacaag gtgaaagtaa tgtagtaatc
      661 ttttggggcg gccctggagc cgcccctttt caacgtggag gtgtgacttg agaaacaccc
      721 ggccccccta ccggatcctg gtcaccggga cccgcggcaa gagtacggct gtgcgctaca
      781 ttgccgcagc cctggactcc atcggtcttt ccttttcggc ccggatcacc gggaccgttc
      841 ccatggtcct tgaaggtctc catgagaaaa ggatcatccg ccactcgccg gcccacctat
      901 cggagatgac ctggtggatc cggagtattc ccgaaggcac gcaggctgtt gtggctgaaa
      961 acagcgccgt ttcccctgag ctgcagcctc tcccgtctaa atggctggac ccgggactga
     1021 tcgtatggac aagcctgctc cccgatcacg cggattgctg gggacctcaa ctttcggggg
     1081 caagggaagc gcttctcaga ggtgttcccg gtaactccag attgctttta ggaaaccagg
     1141 ccggtcagga cctggaactt gtccttgccc tgaaaaagat ggggtgtgac cttttttctg
     1201 tcggacagac caaggaagaa ctcccccgtc agtatgaaag gatcgccgtg gaggcactca
     1261 acatcctcgg tctcccagga tcggaggcct ctttccagaa agttcccagg gatccccatg
     1321 aattcatgat agtgaacccc cgcaggggcg gagcagtagc ctgggcgttc tcctccaatg
     1381 accctgtaac ggccgacgcg ctcttccgtt cgacgggctg ggaccaggag cgaacggcgc
     1441 tgctctttaa ccacaggcac gaccgaccct cccggctttc ggctcacctc ccctggatca
     1501 ggaacggaaa atggaagaga aggatcatca caggcgaccg cccccttatc cccaccgggt
     1561 tcagcttttt tccgcattcc ggtgctgcgg ccctggctga ccttgtcaga agcgagggct
     1621 ccgttttcgg ttgcggcaac gtcaggggcc tcccccttgc acgggaggtc gaagcatcgt
     1681 gaacagggag atactggtaa tagccattgg gatagctctc ggtatgctgt tcttcaaaag
     1741 gactggactc tcccccggcg gcattatctc tccagggatc cttgcccttc acatgaacac
     1801 ttttcacgcc tttgcatgga cccttgcctt ttcgctcctc ctgttcttcc tccttgagat
     1861 atctgttcgc ctcttcggtg tttacggtcg ccagaggatc gcgctgtcgc tgcttttggc
     1921 agccctcacg gctctccttg ctctgggccg attacccctt gaccctcttt ggctcgggtg
     1981 ggtggtaccg ggccttgtgg cctccgatat ccagcgccag gggctattgc ctacattatc
     2041 tgcgctgctt tccctggccg gagtgacttt ccttgccgga ggacttttgc cttgaagggg
     2101 gtcttctcca tcctgaagaa gggcgcaaac cggcagacag caactatcgc tctctgggca
     2161 ctcctggtag ctgcgctggc atggttgccc ggcgacagcc ccctctccca ggaggaatca
     2221 aggctccttc aaaggatcga ggcggcctgg gattcgctgc tggtccttag aagcgaaacc
     2281 ggcgtgcccc actcggagac tgacgacccg cagagatcgg ggatgatagg ggtcgagtgg
     2341 agtaccataa caacaacctc cggtagtctc gcctcgaagc agttgtccgt ccggccagcc
     2401 tgggctgtgg tcttccgaag atggcttgct cgtgagggtc tgggccccgg cgacaaggtt
     2461 accattctca gttcaggctc cttcccgggc cttgcggtgt cttccctgtc cgcggcagag
     2521 tctcttgacc tggacgtaac cctggtgatc tctctgggat cctccacgtg gggggccaat
     2581 attccttcga tgacgatctg tgatatcctc cactttctca ggacaagagg gttcgtacgg
     2641 accagggccg ccgcctgcac catcggcgga gccggggaga tgggaaaaga cctcccgccg
     2701 gaagggcttt cagctctcga ggaagccgct cgaaggggct ttatccccct gatcgccgcc
     2761 aaagatcttg aagagatgat cgaacggaag gcagcggtct ccctgccgga aggaacccgt
     2821 ctcgtgatcc agataggagg gagtaacgct gatctcggca gcgacccctc cctccttgac
     2881 ctccccccgg ggttcctgcg accatctcca gggttgaagg ctggaaacgg cgtggtctca
     2941 aaagccctgg aaaggggtat aactgtcctc catatcctga acatccctga acttgcccgt
     3001 tcggcaggcc ttgaagggag atcctccctg ccatgggaaa gatcgccatg gcggttcctc
     3061 gtcgccgccg cttcaggaat gatcgtactg agttttttcc gacggtggga gtttaaatga
     3121 gaacacctgg agagaggacc attgaaacat ctggaggcaa gtccagccag gctctctggg
     3181 gaacgatact cctggctgcc tggggtgtct cccatttcta tgagaggcct atctaccacg
     3241 tcttccgttt cctcgacctg tccgtaaggc atgacgacag cggctatctg ctggctgcga
     3301 ccgccgtcct tgtagtaatc aacgctgcca gggctctgct cctttacgag gggtggttcc
     3361 tggtaggcaa cggtgtcgcc gaggccactg gtcacagatc ggtggggcgc atcctccccc
     3421 tggtcggtat accggtatgc taccaggccg tggccgtctt cgactttc
//
LOCUS       DAOPKQ010000150         3452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_30552_length_3452_cov_4.792170, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000150 DAOPKQ010000000
VERSION     DAOPKQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30552_length_3452_cov_4.792170"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PK146_05695"
     CDS             <1..460
                     /locus_tag="PK146_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPE91769.1"
                     /translation="IKAVLPAILILALAYCINTISKSLGAQQFIISLTQSWMTAGMLP
                     VITFITGALISFFTGTSWGTYAILTPFVLPVAMNLSGGEINSVVLATVGAVVGGGLFG
                     DHCSPVSDTTCLSSFGAASDHMDHVATQLPYALTAAALSAALFIVIGFIG"
     gene            579..1292
                     /locus_tag="PK146_05700"
     CDS             579..1292
                     /locus_tag="PK146_05700"
                     /inference="COORDINATES: protein motif:HMM:NF005679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPE91770.1"
                     /translation="MTTTGTSPKIGVLCWEAGHVPRGLVQLESLKGNSTNPDSYGFPV
                     RFCRVRGANIQTILENPDMGVLQEMIREARSMISDGVRAITTSCGFNAIFQKELASSL
                     DVPVFTSSLLQVPLVHKMLGPEEEIGVITAKKAALKKEHLEAVGIHEEVSLRVFGLEK
                     CPEWGKIFSDPDMDIDLEAVEEEVTGTAEEAVREYPGIRAFVLECTDLPPFASSIRRS
                     TKLPVFDFISLVELVAGSL"
     gene            1341..2666
                     /locus_tag="PK146_05705"
     CDS             1341..2666
                     /locus_tag="PK146_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPE91771.1"
                     /translation="MKKVLVLGAGRVSRPCVDYLGRLEGTVIHVADVSEENLRQTVSG
                     IPGANIHVCDAGSQGGRLIDEIGPDIVINLLPPAFMPPMAKECLKNRIPLVHPAYLDK
                     ETKDLSKDIAAAGVTFVTELGLDPGIDHMSAARTIDRIRREGGEVESFRSICGAIPSA
                     DANTNPWGYKLSWAPASLIGASKRDARVMIDGRTFEWPDGETYEHVYLEEIEGLGWFE
                     VYANADSLPYLDVYGIPEAKSIYRGTIRYPGWCETICKMNSLGLFEENILDMEGKTFS
                     SFMAEMAGLEEGSDIVSGLCKRLRLEPYSTVMLRFRWLGLLDDGPVPFAKGSPRDVVS
                     RLFAEKLVYDKGEKDLVILQDEFIASFKGKDRKKVFRSTLIDVGVPGGDTSIARTTGL
                     PPAIAARFILEGKIRTPGVHIPVLPEIYGPVMEELERLGVSLKETVTEI"
     gene            2754..>3452
                     /locus_tag="PK146_05710"
     CDS             2754..>3452
                     /locus_tag="PK146_05710"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE91772.1"
                     /translation="MLDLVIRNGNIIDPSDSPFRGNIGIVDGKIVEISTEDIPARQSI
                     DAEGLTVSPGFIDIHMHEDKPQGDSIPYETFNSMALMGVTTVVGGNCGLGEVRVGSYL
                     DIVDRQGSPVNCAVLVGHDVLRQLVGCTDRWGGASLEQVSMMASILREEMERGALGVS
                     FGLEYTPGTSTEEMIQLSRVIAEYPGKMVSAHYRYSTKRSPEALAEMVIVSRETGVKF
                     QISHIGSCCAAGQMS"
BASE COUNT          775 a          920 c         1004 g          753 t
ORIGIN      
        1 catcaaggcc gttcttccgg cgatactgat ccttgcactc gcatactgca tcaacaccat
       61 atccaaatca ttgggggctc agcagttcat catctcactc acccagagct ggatgacggc
      121 agggatgctc ccggtgatca ccttcattac aggcgctttg atctccttct tcaccgggac
      181 ttcctggggt acctatgcca ttcttactcc ctttgtcctt cccgtggcaa tgaatctttc
      241 ggggggagag atcaacagcg tcgttctcgc cactgtgggg gcagtagtag gaggaggcct
      301 cttcggagac cactgctcgc cggtctctga tacgacctgt ctctcatcct tcggggcggc
      361 ttccgaccac atggaccacg ttgcgaccca gctaccctac gccttgacag ctgcggccct
      421 ttcggcagcg ctgttcatcg taatcggttt catcggctag tcctgaagca ggacgacaat
      481 aacgttgcaa aggaagtggc ggtccctggc aggaccgggg accgccgatc ctcagaattg
      541 ttggggatct cattatccca aattgaagga gtgaaacaat gaccaccacc ggcacatccc
      601 cgaagatagg ggtcctctgc tgggaagcag ggcacgttcc cagaggcctt gtccagctcg
      661 agtccctgaa agggaacagc accaaccccg attcctacgg tttccctgtc cgtttctgtc
      721 gggtcagggg tgccaatatc cagaccatac ttgaaaatcc tgacatggga gtacttcagg
      781 agatgatcag agaggcccgt tcgatgatct ccgatggggt gcgggccatc actacgagct
      841 gcggtttcaa cgccatcttc cagaaggaac tggcgtcgtc gcttgatgtg ccggtcttca
      901 cctcgagcct tctgcaggtc ccactggtcc ataagatgct gggcccggaa gaggagatag
      961 gggtcattac tgccaagaaa gctgccctga aaaaggaaca cctggaggcg gtgggcatcc
     1021 atgaggaggt ctcactgagg gtctttggac tggaaaaatg tcctgagtgg ggcaagatct
     1081 tctctgaccc cgatatggat atagatctcg aggctgtgga ggaggaagta acggggacag
     1141 ccgaagaggc agtacgggag tatccgggga tcagggcgtt cgtcctggag tgcaccgacc
     1201 tgcccccctt tgcatcatcc ataaggagat ccacgaagct gccagttttc gatttcatca
     1261 gtcttgtcga gctcgttgcc gggtcccttt gatcgaccgg ccgacaggca gatctttcta
     1321 tcacggagga gtgctttttt atgaaaaagg ttcttgtgct gggcgccgga agggtttcca
     1381 gaccctgcgt agattacctg ggcaggttgg agggtacggt cattcatgtg gctgatgtct
     1441 ctgaggaaaa cctcaggcag acagtctccg gaatccccgg ggcaaatatc catgtatgtg
     1501 atgcagggag ccagggtgga aggcttatcg acgagatcgg gcccgatata gttattaacc
     1561 ttctcccgcc ggctttcatg ccccctatgg caaaagagtg cctgaagaac aggatccccc
     1621 ttgttcaccc tgcctatctg gacaaggaga caaaagatct ttcgaaggat atcgctgcag
     1681 cgggagtcac ttttgtgacc gaactgggcc ttgatcccgg gatcgaccac atgtcagcgg
     1741 ccaggactat agacaggatc cgcagagagg ggggagaggt ggagtccttc cgttcgatct
     1801 gcggggccat cccctccgct gatgcgaata ccaatccctg gggttacaag ctttcctggg
     1861 ctcccgcgag ccttatcggt gccagcaaga gggacgcacg ggtaatgata gacggcagga
     1921 ccttcgaatg gcccgacgga gagacttatg aacacgtcta ccttgaggaa atagagggtc
     1981 tgggctggtt cgaggtctac gccaatgcag actccctgcc ctacctcgac gtttacggaa
     2041 taccagaagc gaagagcatc taccggggga ccatccgtta ccccgggtgg tgtgagacga
     2101 tctgcaaaat gaattccctg ggactctttg aagagaacat tctcgacatg gaaggtaaga
     2161 ccttcagttc ctttatggcc gaaatggccg gacttgaaga ggggagcgac attgtttcgg
     2221 gcctttgcaa gaggctgaga ctggaaccct attcgacggt gatgctcagg tttcgctggc
     2281 tcggtctcct cgatgacggc ccagtgccct tcgccaaggg gagcccgagg gacgttgtct
     2341 cccgcctttt cgcggagaaa ctggtctatg acaagggtga aaaggacctg gtgatcctgc
     2401 aggacgagtt cattgcttcc ttcaaaggaa aggacaggaa gaaggtattc cgctccacac
     2461 tcatcgatgt gggtgttcca ggcggggata cctcaatcgc aagaacaacg gggctgccgc
     2521 ctgctatagc ggcaagattc atcctcgagg gaaagatccg tacaccgggt gtacacatcc
     2581 cggtccttcc cgagatctac ggcccagtca tggaagagct ggaaagactg ggcgtctcgc
     2641 tgaaagagac cgttacagag atctgatcca ccggaggata gccctccctt atcacggaac
     2701 aggacgcggc ggggcttcaa caccaacttt tcaaccacga aaggagggcc accatgctgg
     2761 accttgttat caggaacggg aacatcattg atccttcgga tagtcccttc aggggtaaca
     2821 tcgggatcgt cgacggaaag atcgtcgaaa tatctacaga ggatattcct gccaggcagt
     2881 ccatcgacgc cgaggggttg accgtgtctc ccggatttat tgacattcac atgcacgaag
     2941 acaaacccca gggtgactcg atcccttatg agaccttcaa cagtatggca ctgatgggcg
     3001 tgaccaccgt cgtaggaggt aactgcgggc ttggggaggt ccgtgtcggc agctatctcg
     3061 atattgtcga ccggcagggg tctccggtga attgcgccgt actggtgggt cacgatgtac
     3121 taagacagct tgtcggctgt accgacagat gggggggagc ctccctggag caggtctcca
     3181 tgatggcctc gatcctccgg gaagagatgg agaggggtgc actgggagta tctttcggtc
     3241 ttgagtatac ccccggtaca tccaccgagg agatgattca gctcagcagg gttatcgcag
     3301 aatacccggg gaagatggtc tccgctcatt atcgctacag cacgaaaaga tcccctgaag
     3361 ctttggccga aatggtcatt gtatcccggg aaacgggcgt gaagttccag atatcccata
     3421 tcgggagctg ctgtgcagca ggtcagatgt cc
//
LOCUS       DAOPKQ010000151         3395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_31257_length_3395_cov_4.338922, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000151 DAOPKQ010000000
VERSION     DAOPKQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31257_length_3395_cov_4.338922"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1273)
                     /locus_tag="PK146_05715"
     CDS             complement(92..1273)
                     /locus_tag="PK146_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPE91773.1"
                     /translation="MDLTELLIHASDVDEQTMAQIRGIASHPAIEGLVAIMPDCHLGA
                     GCVIGFTGRFSEAVIPNVVGVDIGCGVVLNPIEGVERREIDFDALDRFIRSSIPLGMT
                     HHRTTALLEWFGSRAPEKYAMAKDLVARADKEFHGKLLSRRPRYPAWGQLGTLGGGNH
                     FIEIDEDQEGSLFLTVHSGSRHFGFQVANFFQDMARRFQSRSRESDGLPRGLEHLPLD
                     KGGREYLKWLRIAQEFALMNRSIIAERILSFFGKGLDERSLVESVHNYISERDGIVRK
                     GAISAHKGERVVIPLSMASGVVLGIGKGNPSFNYSAPHGAGRLFGRKEMKRRLKEGAI
                     TMGSFKKSMEGVFSTSLSSETIDESPMAYRRWEDISDEVKETVEVTALLKPVYNLKSP
                     E"
     gene            1366..2169
                     /gene="nudC"
                     /locus_tag="PK146_05720"
     CDS             1366..2169
                     /gene="nudC"
                     /locus_tag="PK146_05720"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPE91774.1"
                     /translation="MDPKDLASIFSPENRTCFAFSGDQLLLRTDPEPSLPAREDLSEL
                     PLSGLSPRCGLPGRPSNCWVSLAGNAPIPEGMSLVSLRDVWSLLGEQAFFEAGRAFQL
                     MEWSCLNRFCGRCATLMREVEHETARECPACGTITYPPVSPAIIVAVEKNGKLLLARS
                     PRFPRGRYSILAGFVEPGETLEGAVEREVMEEVSIRVKNIAYFGSQPWPFPHSLMVGF
                     RAEWGSGDIRVDGNEIESAGWYSPGSFPDIPPSLSISRKLIDDFLARHG"
     gene            complement(2199..>3395)
                     /locus_tag="PK146_05725"
     CDS             complement(2199..>3395)
                     /locus_tag="PK146_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91775.1"
                     /translation="EAQELTMAPEEGVLVRTAISLRDVRKSYGDTEVVKGVDLDIAGG
                     EIMVLIGPSGCGKTTILRMINRLVEPSGGTILCEGQDTSRMDPVALRRRIGYVIQGIG
                     LFPHMRVLENVSVVPRLKGIGPDEARSISGRALEMVGLPPSEYGEKWPRQLSGGQQQR
                     VGVARALAGDPEIILMDEPFGALDPISRIGLQDELLDLQFKLKKTVVFVTHDISEAMK
                     MGNRIAIMRSGELVQVGEPLALLSSPDDDFVSGFVGAGNPMAMFSFMKVKDVPVRTSD
                     LPVVDIEDSMSNAVRAAHNTDLRFCRDRFVYVLDQGRLAGSVNVEGIPPVALPGPVRE
                     RLQPASSVRTTRTVQEALGTMISSAIANVAVVDEAGRFLGVLSFSDMYDIIQQQKERD
                     GTTAVV"
BASE COUNT          723 a         1043 c          888 g          741 t
ORIGIN      
        1 ggacccaggg ttctcagtcc tgtaacacgg gttcgaatcc cgttggggct accagagttg
       61 cgaggcggac acctgaatgg tgtccgcctt ttcattcggg ggacttcagg ttgtagaccg
      121 gcttgagaag ggcagtaacc tcgacggtct ccttgacctc atcactgatg tcctcccacc
      181 gcctgtacgc catgggagac tcatcaatgg tctcgctact taaagaggta gagaagacac
      241 cctccatgct ctttttgaaa gatcccatgg ttatggcgcc ctccttcagc ctccgtttca
      301 tctccttgcg cccgaacagg cgccccgcac catggggtgc ggagtagttg aaagaggggt
      361 ttccctttcc aatacccaga acgacccctg acgccatgct cagggggatg accaccctct
      421 ctcccttatg ggccgaaatg gcgcctttcc tcacaatgcc gtcccgttcg ctaatgtagt
      481 tgtgcacgct ctcaacgagg gacctctcat ccagtccttt cccaaagaag gaaagtatcc
      541 tttctgcgat aatggaacgg ttcatcaggg caaactcctg ggcaatccga agccatttca
      601 aatactctct ccctcccttg tcgagaggga gatgctccag gcccctgggg agaccgtccg
      661 actccctgct cctgctctga aaacgccgag ccatgtcctg aaagaaattc gcgacctgga
      721 acccgaagtg gcggcttccg ctatggaccg taaggaagag agatccctcc tgatcttcat
      781 cgatctcgat aaagtgattg ccgcccccga gagtccccag ttgaccccat gcaggatacc
      841 tgggtctcct tgaaaggagt tttccgtgaa actccttgtc agcccgggcg accaggtcct
      901 ttgccatggc gtacttctcc ggagcacggg atccgaacca ttcgagcaag gccgtggtcc
      961 tgtgatgggt catacccagg ggaatactgc tccggatgaa cctgtcaagg gcgtcgaagt
     1021 cgatctcccg tcgttccact ccttcgatgg ggttcaggac cacaccgcag ccaatgtcta
     1081 caccgacaac gttgggtatc acagcctcac tgaatctccc tgtaaaaccg atcacgcagc
     1141 ccgctccgag gtgacagtca ggcattattg ccaccagccc ctcgattgca gggtgggagg
     1201 cgatgcccct tatctgggcc atggtttgtt cgtccacatc actggcatgg atcaacagtt
     1261 cggtcaagtc catcaccctc ggtcctcctc tgaaggaaag ctcaaaccag ccccggggtc
     1321 atgataatat ctagaatacc agacatcgtg acggaggaag ataacatgga cccaaaagac
     1381 ctggcatcta tcttttcacc tgagaacaga acatgcttcg ccttcagcgg agaccagttg
     1441 ctcctgagga ccgaccccga accttcactc ccggcaaggg aggacctgtc ggagcttccc
     1501 ttgtccgggc tgagccctcg ctgcgggttg ccggggcggc caagcaactg ctgggtctct
     1561 ctcgccggca atgctcccat ccctgaagga atgagcctgg tatctctgag agatgtctgg
     1621 tcactgctgg gcgagcaagc cttcttcgaa gccgggaggg cgttccagct aatggaatgg
     1681 tcctgcctta accggttctg cggaaggtgc gcgacactca tgagagaggt cgaacacgaa
     1741 accgccaggg agtgccctgc ctgcggaacc atcacttacc cccctgtatc cccggccatt
     1801 atcgtcgccg ttgagaagaa tgggaagctg ctgctggcga gaagcccccg tttccccagg
     1861 ggaaggtaca gtatactggc aggttttgtt gaacccgggg agaccctcga gggtgccgta
     1921 gagagggagg tcatggaaga ggtctcgata agagtgaaga acatcgcgta ttttggaagc
     1981 cagccatggc cctttccgca ctccctgatg gtggggttcc gggctgagtg gggatcgggg
     2041 gatatcaggg ttgacggcaa cgagatcgag agtgccggat ggtactctcc aggttctttc
     2101 cccgacattc ctcccagcct cagcatttca agaaaactta tcgacgactt cctggccaga
     2161 cacgggtgat caacctttcc gccaaccggt gtgagctatc acacaacagc tgtcgtcccg
     2221 tccctctcct tctgctgctg gattatgtca tacatgtcac tgaaggagag aacccccaga
     2281 aaacggcccg cttcatcgac aacagccacg ttggcaatgg ccgaggatat cattgttccc
     2341 aatgcctcct ggactgtcct tgtggtcctc acgcttgaag ctggctgaag cctttcacgg
     2401 accggcccag gcaaggccac gggggggatg ccttcgacgt tgacgctacc ggcaagcctc
     2461 ccctggtcga gaacgtatac gaaacggtcc ctgcagaacc tcaggtcggt attgtgggcc
     2521 gccctgacag cattggacat gctgtcctct atatccacca ccggcagatc cgatgtcctg
     2581 acaggaacat ccttcacctt catgaagctg aacatggcca taggattacc agcgcctaca
     2641 aaccctgata cgaagtcgtc gtccggtgat gacagaagag cgagaggttc ccccacctgg
     2701 acaagttctc ctgacctcat tatggcgata cggttaccca tcttcatggc ctcgctgata
     2761 tcatgggtga cgaagacaac tgtctttttc aacttaaact gcaggtccag cagctcgtcc
     2821 tgaaggccga tcctcgagat aggatcaagg gcgccaaagg gctcgtccat aaggatgatc
     2881 tccggatctc ctgccagggc ccgagccacc cctactctct gttgctgccc tcccgatagc
     2941 tgtcttggcc acttctcacc gtactcggac ggcggtagac caaccatctc cagggccctc
     3001 ccggagatcg aacgggcctc atcgggacca atacccttga gcctgggtac caccgatacg
     3061 ttctcgagga ccctcatatg agggaatagc ccgatcccct ggatcacgta tcctatccgc
     3121 cttctcaggg caacagggtc cattctggac gtgtcctgac cctcacagag tatggtgccc
     3181 cccgagggtt ccaccagcct gtttatcatg cggagtatgg tcgtcttccc gcagccggag
     3241 ggacctatca ggaccattat ttcaccaccg gcaatatcaa ggtctacccc tttaacgacc
     3301 tccgtatcgc cgtagctttt cctgacatcc ctgagactta tggccgttcg aaccaaaact
     3361 ccctcctccg gggccatggt cagctcttgg gcttc
//
LOCUS       DAOPKQ010000152         3392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_31300_length_3392_cov_3.653881, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000152 DAOPKQ010000000
VERSION     DAOPKQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31300_length_3392_cov_3.653881"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PK146_05730"
     CDS             complement(<1..604)
                     /locus_tag="PK146_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE91776.1"
                     /translation="MDHRIYDLEGIDVQWCPGCGDFQIMEALKKALAGLDLPPERVVL
                     FSGIGQAAKMPHFMKGHFFNGLHGRSISNATGMKVANRDMTVIAIGGDGDMYGEGGNH
                     FIHTIRRNPDITNLVHNNMVYGLTKGQASPTSPVGFKTSIQVDGVFSEPFNPVAVAVS
                     MGASFVARAFSGNVEQTTEIIKKAIIHKGYSLVDIFQPCVS"
     gene            complement(616..2343)
                     /locus_tag="PK146_05735"
     CDS             complement(616..2343)
                     /locus_tag="PK146_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPE91777.1"
                     /translation="MFRFPAEGNSDVSLVLCGAAGQGVQTVEEILVDSLRKSGFNVFA
                     SREYMSRVRGGSNSTEIRVSSERVRAFVDRIDILLPLNGGIRANIRGRISQETLILGD
                     AEEIRDELSDSGGTFVHVPLMEKGREAGGPVFTGVVAAGLISGLLGIPPEIPERLLRE
                     KFSSKSEGVVTSNLSALEKGMGLFRELETSGRVSVEVRGRGKASGEMMINGSDAVSLG
                     ALAGGCDLISSYPMSPATGVLTFMARHSRSHGVAVEQAEDEIAAINIALGASYGGARA
                     MVSTSGGGLSLMSEGVSLAGITEIPLVLHIGQRPGPATGMATRTEQGDLNLAVFCGHG
                     DIPRAVFAPGSMERAFRLTFEAFNLAAEFQVPVFILTDQYLLNSFYNVPPFEIKGLNV
                     ERHLVESGADYRRYEVTESGISPRSVPGLGKGLVGTDSHEHDEVGHVQEDFVLRPRMV
                     DKRQRKRGGILERVFEPVISGPESYRNLVVTWGSMYHIVEEALQEARLDETAHLHFDQ
                     VFPLHPGTAGHLKKAERVIAVEGNATGQFADLIRRETGFLIEDRILWYSGLQVPVEKV
                     AEKLIEMLR"
     gene            complement(2460..2657)
                     /locus_tag="PK146_05740"
     CDS             complement(2460..2657)
                     /locus_tag="PK146_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPE91778.1"
                     /translation="MRKVHSYRVPGMSCGHCVKRITGVLESMGVTDFEVSLEEKRILS
                     GTPLGEEVIQALEEAGYPVTK"
     gene            complement(2658..>3392)
                     /locus_tag="PK146_05745"
     CDS             complement(2658..>3392)
                     /locus_tag="PK146_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPE91779.1"
                     /translation="ASAGGDLPVAEEVEETAGKGVSGVVLGARYEIGRPSSYEPYFEF
                     LETGKTVVEVIREGFAAGFFVIEDRVREDSRSAVEDMKSLGLLPVMVTGDNEATARAV
                     ALQVGITEVHSGVRPEDKLDIVRTYQAGDRKVLMIGDGINDAAALKGADIGMAIGSGT
                     DLAIDSADVVVMSGGVSRVLECVRISRRTFRVITRNLFWAFLYNVVAVPVAMAGLLHP
                     AIAEAAMALSSISVVLNSLTIRKGDI"
BASE COUNT          712 a         1086 c          853 g          741 t
ORIGIN      
        1 aggacacaca gggctggaat atatccacca gtgagtagcc cttgtgaatt atcgcctttt
       61 ttatgatctc cgttgtctgt tccacgttcc cggagaaggc ccttgcaaca aaggatgccc
      121 ccatcgaaac agctactgca acagggttga agggttccga gaagaccccg tccacctgga
      181 tggaggtctt gaaacccacg gggcttgtcg gggaggcctg cccctttgta aggccgtaga
      241 ccatgttatt gtgcaccagg ttagtaatat ctgggtttct ccttatagtg tggataaagt
      301 ggttcccgcc ctcaccgtac atatccccgt ccccgccgat ggcgataacc gtcatatccc
      361 tgttggcgac cttcattccc gtggcattgg agatcgaacg tccatgaaga ccgttgaaaa
      421 agtgcccctt catgaagtga ggcatcttgg ccgcctgccc tatcccggaa aagaggacca
      481 ctctctccgg gggaagatca aggccggcaa gggctttttt cagtgcttcc atgatctgga
      541 aatccccgca tccggggcac cactggacgt caattccttc caggtcgtag atcctgtgat
      601 ccatctctct cccccctatc gaagcatctc aatgagcttt tcggcaacct tttcgacggg
      661 aacctgaagc ccactgtacc agagaatacg gtcctcgatg aggaaccccg tctccctcct
      721 gatcaggtca gcgaactgcc cagtggcgtt gccctcgaca gctatgaccc tttcggcctt
      781 cttgaggtga ccggcagttc caggatgaag cgggaacacc tggtcgaagt gaaggtgggc
      841 cgtctcatcc aaccgggcct cctgcagggc ttcctcaact atgtgataca tggaccccca
      901 ggtcaccacc aggttcctgt agctttcagg ccccgaaata acaggctcga acaccctctc
      961 caggatgccc cccctcttcc tctgccgctt gtcaaccatt cggggacgga gtacgaagtc
     1021 ctcctggaca tgtcccacct catcgtgttc atggctgtcg gtaccgacaa gtcccttgcc
     1081 cagaccgggg acactcctgg gagatatgcc gctctcagtg acctcgtacc tcctgtagtc
     1141 ggcgccggac tcaacgaggt gcctttcaac attcagaccc ttgatttcga aggggggaac
     1201 gttgtagaag gagttgagca ggtactggtc ggtaaggatg aatacgggga cctgaaattc
     1261 agcagccagg ttaaaggcct cgaaggtcag gcgaaatgcc ctttccatgc tcccaggagc
     1321 gaagaccgcc cgggggatgt cgccgtggcc gcaaaacacc gccaggttca ggtcaccctg
     1381 ttcggtcctg gtcgccatac ccgtggcagg tcccggccgc tgaccgatat ggagcaccaa
     1441 ggggatctcc gttatgcccg caaggctgac cccttctgac atcagggaga ggccgccccc
     1501 ggaggtgctc accatggccc tggctccacc gtaggacgca cccagggcta tgtttatggc
     1561 ggctatctca tcctctgcct gctccacagc tacaccgtgg gaccttgagt gccttgccat
     1621 gaaggtaagg actcccgtgg ccggggacat cgggtaggac gagataagat cgcaacctcc
     1681 agcgagggcc cccagagaga cggcgtccga tccgttgatc atcatctccc cggaagcttt
     1741 ccctctcccc ctaacctcga cggagaccct ccccgaggtt tccagctctc tgaagagtcc
     1801 catccccttt tcaagggccg aaaggttgct ggtgaccacc ccttcggact tggaggagaa
     1861 tttttccctg agcaaccttt cggggatctc gggaggtata cccagcagtc ccgaaataag
     1921 tcctgccgcc acaacgccgg taaagaccgg cccccctgct tctcgcccct tctccatcag
     1981 agggacgtgg acgaatgtcc ctccggaatc cgagagctcg tccctgatct cttcggcatc
     2041 tccgagaata agggtctcct gggagatgcg tcccctgatg ttcgccctta tccccccgtt
     2101 gaggggcagc agaatgtcga tcctgtcgac aaaggctctc accctttcgg aggagacccg
     2161 tatctcggtt gagttgctcc cccccctgac ccttgacata tactccctgc tggcgaaaac
     2221 gttgaaaccg gacttcctca gggaatcgac caggatctcc tccaccgtct ggaccccctg
     2281 tcctgccgca ccgcacaaaa ccaacgacac gtcgctgttt ccttctgccg ggaaacggaa
     2341 catactatct cctcccaact ttaaaaagga acgaagtgtt atctcgcatc aaacatacgg
     2401 ccatgggcct gggacattac agaactggcc tgacatgtca gttatcctgt gtaacggagt
     2461 tactttgtta cggggtatcc ggcctcttca agggcctgga taacctcttc gccaaggggt
     2521 gttcctgaga gaatcctctt ctcctcaaga gaaacctcga agtcagtcac ccccatcgac
     2581 tccagaaccc ctgttatcct cttgacgcaa tgaccgcagg acatgccggg caccctgtag
     2641 gaatgaacct ttctcattca gatatccccc ttcctgattg taagcgagtt aagcaccacc
     2701 gagatcgagc tcaaagccat ggccgcttct gcaattgcag ggtggagaag acccgccatt
     2761 gcgaccggta cggcaacaac attgtacaga aaagcccaga acaggttcct tgtaattacc
     2821 ctgaaggtcc ggcgtgatat ccggacacac tccaggaccc tcgatacccc cccgctcatc
     2881 acgactacgt cagcactgtc gatggcgagg tcggtcccgg agcctatggc catccctata
     2941 tcggccccct tgagggccgc ggcgtcattt atcccgtcgc ctatcataag caccttcctg
     3001 tcacctgcct ggtaggtcct cactatatca agcttgtcct cgggcctgac ccctgaatgg
     3061 acttcagtaa tgcctacctg gagtgccacg gccctggctg tagcctcgtt gtcccccgtt
     3121 accattaccg ggagaagtcc aagggacttc atatcctcca cggcggacct ggaatcttcc
     3181 ctgaccctgt cctctatgac gaagaagcct gcggcaaacc cttccctgat cacctcgacc
     3241 accgtcttgc ctgtttcgag aaattcaaaa tagggttcat aggatgacgg tctgccgatt
     3301 tcgtatctcg caccgagaac cactcctgag actcccttgc cggcggtctc ctccacctcc
     3361 tcggcgaccg gaaggtctcc accggccgag gc
//
LOCUS       DAOPKQ010000153         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_31714_length_3361_cov_4.620992, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000153 DAOPKQ010000000
VERSION     DAOPKQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31714_length_3361_cov_4.620992"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..807)
                     /gene="deoC"
                     /locus_tag="PK146_05750"
     CDS             complement(79..807)
                     /gene="deoC"
                     /locus_tag="PK146_05750"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPE91780.1"
                     /translation="MTDIELLSGRARLKAAKAEAGFFEHPPEDRIRRSIDHTLLSAGT
                     TADDVAILCDEAVRFGFASVCVPLNCVTDARRSLSGTEVKVSSVASFPLGASPIPVKT
                     AEVRWALENGAEEMDVVLDIRAVRTDDKRAVREDLAALRDVSRGAVLKVILEMPILND
                     AQAVTALLLAEQAGVDYVKTCTGFAGGVTFYHVALLKTAASDRVRIKASGGIRTYAAA
                     EILVALGADRLGTSKATQFLTPLA"
     gene            complement(813..1172)
                     /locus_tag="PK146_05755"
     CDS             complement(813..1172)
                     /locus_tag="PK146_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91781.1"
                     /translation="MGENKDTQEMDFSENYAKAAARVLESISRTAQLSSLATPEIQEM
                     FENWLEILSHQILRSQEIPGRLDIPSKAEEIGISSSSLLGLLLYLQRQGRISITEIQL
                     ESGCGLDEDICDCFRER"
     gene            1321..2361
                     /locus_tag="PK146_05760"
     CDS             1321..2361
                     /locus_tag="PK146_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPE91782.1"
                     /translation="MRILLLGFGNIGRAFCEIVEEKAPVLREKYGIECRFVGIATRSR
                     GCTTDPNGIDVEALLKRESSTGRLDTPSSGRRRATLSASEMVSEMDYDLLVICTSTTI
                     QSPEMPKAYIRGGLERGKDVITSNKGPLFLAYRELSDLATERGALFLFEAAVMGGTPF
                     FSFARHSLVGSEIISCEGVMNTTSNYILDLMVGGSSFDDALLDARRKGFAEADPAFDL
                     EGWDSAVKASIIAQVLMGAPPVSLESMNVQGINKIKTGLLLEAARSGGAVRLISRVER
                     EEGSVSITVKPEVLSPSNPLSRVHGATNGVIFLTDTLGEIFVAGGGIGPKHTAYSMLR
                     DLIEIAGSRKTR"
     gene            2443..>3361
                     /locus_tag="PK146_05765"
     CDS             2443..>3361
                     /locus_tag="PK146_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HPE91783.1"
                     /translation="MTSFSSEAIRGDDIDKVLLKWEPKAWRINRRIFARPETGHNERL
                     ASSLWASLLEEGGFNVQMPFQGMETSFFAVMGSGPQKYVLLVEYDALPDIGHGCGHNL
                     SGAMSVLAALALGDLPGIEGEVRVIGTPAEESGGSKVILAESGFMDDVDLAAMIHCGN
                     ATAVPFRSLAIDAQRFTFEGGEASDALMFFLHCLDMIRDDLPEGVSIHGIVRHDKGAG
                     VDLYIRAPERDLLERIAGRVKRCAEGASKATGSFLGHDRFEPSFENMLPNDSAENLLE
                     RLFMLEGERPSAEVGYGGSSDIGNISHRCP"
BASE COUNT          735 a          940 c          966 g          720 t
ORIGIN      
        1 tcccagtcat aagtcacgag ttaagagtaa cgattcacga gttacgattc acgattcacg
       61 atcaacgggt tcccctgttc acgccaacgg tgtcaaaaac tgcgtcgcct tcgatgtccc
      121 gagcctgtcc gccccgaggg cgacgaggat ctccgcagca gcgtaggttc taatgcctcc
      181 ggaagccttg atccttaccc tgtcagacgc ggctgttttg agaagggcca catggtagaa
      241 ggtgaccccc cccgcgaacc cggtgcaggt cttaacgtaa tccaccccgg cctgttcggc
      301 cagcagcagg gcggtcaccg cctgggcatc gttaaggatg ggcatttcca ggatcacctt
      361 cagcacagcc cccctggaga cgtcccgcaa ggctgcaagg tcctcccgga ccgccctctt
      421 gtcgtcggtc ctgacggccc tgatatccaa aacgacatcc atctcttccg ccccgttctc
      481 gagggcccaa cgaacctctg ctgtcttgac cggtatgggg gaggctccca ggggaaagga
      541 cgctactgaa ctgaccttga cctcggtccc cgagagagaa cgccttgcat cagtgacgca
      601 gttaagaggg acgcagaccg atgcaaaacc gaatcgtact gcctcgtcac agaggatggc
      661 cacgtcatcg gccgtggtcc cggcagaaag aagggtgtga tcaatggacc tgcggatcct
      721 gtcctcggga gggtgctcaa aaaaaccggc ctccgccttt gctgccttca gcctggcccg
      781 accggagagg agttcaatgt cggtcatgga gatcacctct ccctgaagca gtcgcagata
      841 tcttcatcaa ggccgcatcc ggattccagt tggatctccg taatggatat cctgccctgt
      901 ctctgcaagt aaaggagcaa tcccagcagc gatgatgacg agatccctat ctcttcggcc
      961 tttgagggga tatccagtct tccggggatc tcctggctcc ggaggatctg gtggctcaat
     1021 atctcgagcc agttctcgaa catttcctgt atctccggtg tcgccaacga tgaaagctgg
     1081 gcggtcctcg agatcgactc cagcaccctg gcagccgctt tggcgtagtt ttcgctgaag
     1141 tccatttcct gggtatcctt gttctctccc atctctttca ggccccctgc agttcgtctt
     1201 gtgctgagga catgcccctt ccagggatct atccggggcg tcctctctga gagatcagaa
     1261 gcaatgatat catagacgtt gatcacagga acggaagacg gtcaggaggt gcacagggca
     1321 ttgaggatcc ttctactggg tttcggaaat atcggaagag cgttctgcga gatcgtcgaa
     1381 gagaaggccc ctgtcctcag ggaaaagtac gggatcgaat gccgtttcgt gggtatagct
     1441 acacgatcga gaggatgtac aacagatccg aacggcatag atgtggaagc ccttctgaag
     1501 agggagtctt ccacgggaag gcttgacaca ccttcctccg gaaggaggcg ggcaaccctt
     1561 tcagcttccg agatggtctc ggaaatggat tacgaccttc ttgtgatctg cacctccacg
     1621 accatccagt cccccgagat gccaaaggcc tacatcaggg gcggacttga gcggggaaag
     1681 gatgtgatca catccaacaa gggccctcta ttccttgcct acagggaact cagcgatctc
     1741 gccaccgaaa gaggagctct ctttctcttc gaagcggcag taatgggcgg gacccccttc
     1801 ttctcctttg ccaggcactc ccttgtgggg tcggagatta tatcctgtga aggcgtcatg
     1861 aacactacct ccaactacat ccttgatctc atggtgggtg ggtcttcctt cgatgacgcc
     1921 cttctcgacg cgagaaggaa gggtttcgca gaggctgatc ccgcctttga cctggaggga
     1981 tgggacagcg cagtcaaggc ctcgataatc gcacaggtgc tcatgggcgc cccgccggta
     2041 tctctggaga gcatgaacgt ccagggcatt aacaagatca agaccggact cctccttgaa
     2101 gcggcccgtt caggaggggc cgttcgcctg atctcaaggg tggaaagaga ggagggttcg
     2161 gtctccataa cagtaaaacc cgaagtcctt tcgccatcga atcctctttc aagggttcac
     2221 ggagcaacca acggggtgat tttcctaacg gacacactgg gtgagatctt cgtcgccgga
     2281 gggggtatcg gtccgaagca tacggcctat tccatgctga gggacctgat agagatagca
     2341 ggatcgagga aaacccggtg acgacgagaa aacaacaagt cttcctgtca gaagacctca
     2401 tgggaatatt gatcctcccg gcgaaggagc ggaccggcag tgatgacatc tttctcttca
     2461 gaagccatta gaggggacga tatcgacaag gtccttctca agtgggaacc aaaggcctgg
     2521 aggatcaacc gtcgtatctt cgccagaccc gagacgggtc acaacgaaag actggcttcg
     2581 tccctgtggg cgtcacttct tgaagagggc ggtttcaatg tgcagatgcc tttccagggg
     2641 atggagacct ccttctttgc ggtcatgggg tcagggccgc aaaagtatgt cctcctggtg
     2701 gagtacgatg ccctgcccga tattggccac ggatgcggtc ataatcttag tggcgccatg
     2761 agcgttcttg cagcgctggc cctcggcgat cttcccggga tagaaggcga ggtccgcgtc
     2821 atagggactc cggcagagga gagcggaggt tccaaggtca ttcttgccga gagcggtttc
     2881 atggacgatg tggacctggc tgcaatgatc cactgcggga acgccactgc agtccccttc
     2941 agatcgcttg ccatcgatgc ccagaggttc actttcgagg gtggagaggc ttctgacgcc
     3001 cttatgttct tcctgcactg tcttgacatg atcagggatg atctgcccga gggagtgtcc
     3061 attcacggta tagtcagaca cgacaagggg gcgggggtcg acctgtatat ccgcgcccct
     3121 gaaagggacc tccttgagag gatcgccgga agggtcaaaa gatgtgccga gggtgcctcg
     3181 aaagcgacag gctcctttct ggggcatgac cgttttgaac cctcctttga gaacatgctt
     3241 ccgaacgatt cggcggaaaa tctcctggaa agactcttca tgctggaggg agaaagacct
     3301 tccgctgaag tgggttacgg gggaagcagc gacatcggca acatctccca caggtgcccg
     3361 g
//
LOCUS       DAOPKQ010000154         3359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_31741_length_3359_cov_3.962772, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000154 DAOPKQ010000000
VERSION     DAOPKQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31741_length_3359_cov_3.962772"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(484..1020)
                     /locus_tag="PK146_05770"
     CDS             complement(484..1020)
                     /locus_tag="PK146_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPE91784.1"
                     /translation="MVSERTVGRTVIRLRKGDITAFEGEAIVNAANSSLWMGSGVAGA
                     IRRLGGESIEREAMGKAPIEPGEAVVTDAGALKARYVIHAAAMGDDLATSEALIRAAT
                     QNSLYKCDELNIRSVAFPALGTGVGRFPVDKCAHAMIDEVFLYLQEETDTSLREIVFC
                     LFGEETYQSFSKVLEKRG"
     gene            complement(1163..2602)
                     /gene="proS"
                     /locus_tag="PK146_05775"
     CDS             complement(1163..2602)
                     /gene="proS"
                     /locus_tag="PK146_05775"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPE91785.1"
                     /translation="MARNIVSREKDYSQWYLDVIKAAELADYAPVRGCMIIRPDGYAL
                     WEFIQKHFDEAFKDTGHVNAYFPLLIPMSFLEKEAEHVEGFAPECAVVTHAGGEELEE
                     PLIVRPTSETVIGHMYSKWVQSWRDLPILINQWCNVMRWEKRSRLFLRTSEFLWQEGH
                     TAHATREEAVEETLKMLGVYRDIMENVLALPVLAGEKSEGERFPGADNTYACEAMMSD
                     RKALQAGTSHFLGQNFARAFDISFQNSDGELDFAWTTSWGVSTRLIGAIVMTHSDDDG
                     LVIPPRVAPVKTVIIPISPDETFLESDLLPRAREAQEKLNVVLGKRSVKIDTAFHMRP
                     GDRFFANIQKGIPLRLEMGEKEFERGVFQAVRRDSGERFDIPWEEVEERVPRVLDEIQ
                     NDLYLKALEFRKQNTFRVSDLEGFKEVFAARGGFVEAFFAGGREEEKLIKEETGATVR
                     CFPMDNDESGTCFFTGKPGARLAVFARSY"
     gene            complement(2718..>3359)
                     /locus_tag="PK146_05780"
     CDS             complement(2718..>3359)
                     /locus_tag="PK146_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91786.1"
                     /translation="YSLALSGINSRGQFRASLGYAGEFIHFDGEERTHHGPLLTLAWD
                     GLDDPFDPAEGFSASLSAWWMDTSSLLARMDLLWVTPLGERWRLLVKGGATAGDASRP
                     YHSVYLGTREELLSLASRPLAGENAAWGGMAFRRVFQRSWWGTVNLDIIANVGQSYGP
                     SWERLESAWEAGLALSLPGRFLNGRLLVIYDDRSEWTFGFTIGRPFWDTDPLP"
BASE COUNT          706 a         1025 c          899 g          729 t
ORIGIN      
        1 aattgctcga gtctcgcccg ctccttctcc tgcctatata actatgtcaa ggtgcatgaa
       61 aaaacgccgc tcgaggcttc gccttccacg gcgcgcaagg ggtataatag cgagcctttc
      121 aaaagaagtc aaggacttat ttccaggttc gtgaactgta aatcgtgaat cgtaaatcgt
      181 gggagggaaa cagacagaac ttgaactgtc gggagcttta ccgggaacaa aggagccggt
      241 gagtgacagc accgtagaaa aggggcaggg aaagcgcagg agggtgtcat ttgctcggct
      301 ttgcgcggtc gccccgcagc agacacatgc acgcacggtt tcacccgagt cgttcccggg
      361 aaagtcttcc ggcaggctcc gattcacaac ttacgatccg cgggtttctc tgttgactgt
      421 ttactgttca ccgttaacgc tcttgatctg ccgggttacg cgttacaagt tacggacctt
      481 gacctaaccc ctcttctcca ggaccttgct gaaggactgg taggtctcct ctccgaagag
      541 gcagaagacg atctcgcgca aggaggtgtc ggtctcctcc tggagataga gaaagacctc
      601 gtctatcatg gcatgggcgc acttgtccac cgggaatctc cctacccccg tccccagggc
      661 ggggaaggca acgctccgga tattgagttc gtcgcacttg tagaggctgt tctgggtggc
      721 agccctgatg agggcttcgc tggtggcaag gtcgtcgccc attgccgcgg cgtgtatcac
      781 gtacctggct ttcagtgctc cggcatctgt gacgacggct tcccccggct caatgggcgc
      841 tttgcccatt gcctcccttt cgatggattc accaccgagt ctcctgatgg cgccagccac
      901 ccctgagccc atccagaggc tggagtttgc ggcattgact atcgcttctc cctcaaaagc
      961 agtgatatcc cccttgcgga gccggatgac cgttcggccg acggttcgct cggataccat
     1021 gttctctcct ccctgtccgt tccaccctcc atctatcagc aaacagggtc acggaattgc
     1081 cttcccttac tagtatatga caaaggggac ctccccggga taaggaagag cccctttgat
     1141 tccttcgaga agaggaaccc gatcagtatg atcttgcaaa aactgccagt ctggccccgg
     1201 gtttgcccgt aaagaaacag gtaccggact cgtcgttgtc catggggaag cagcgaaccg
     1261 tagctcccgt ctcctccttg atgagtttct cctcttcccg gcctccagcg aagaaagctt
     1321 ccacgaatcc tcccctggca gcgaatacct ctttgaaacc ttcaaggtcg ctgacccgga
     1381 aggtgttctg cttccggaac tcaagggcct tcaagtatag atcgttctgg atctcatcca
     1441 ggaccctggg aaccctttct tccacttcct cccaggggat gtcgaagcgt tctccactat
     1501 ccctccgaac agcctgaaaa acccctctct caaactcctt ctctcccatt tccaggcgaa
     1561 ggggtatccc cttctggatg ttcgcgaaaa agcggtcccc cggcctcatg tggaaggcgg
     1621 tatcgatctt gacggacctc ttgccaagaa cgacattgag cttctcctgc gcttccctgg
     1681 cccttggaag aagatcgctt tccaggaatg tctcatcggg gctgatgggt atgatcacgg
     1741 tctttaccgg agccactctt ggaggtatca ccaaaccgtc gtcatcggag tgggtcatca
     1801 caatggcacc tatcagcctg gtcgagacac cccagctggt agtccaggcg aaatccagct
     1861 cgccgtcact gttctggaaa gagatgtcga aggccctggc gaagttctgc cccaggaagt
     1921 ggcttgtccc tgcctgtaga gccttcctgt cgctcatcat ggcctcgcag gcgtaggtgt
     1981 tgtctgcccc cgggaatctc tccccttcgg acttctcacc tgccagaacg gggagtgcca
     2041 gaacgttctc catgatgtcc cggtatactc ccagcatctt cagggtctct tccacggcct
     2101 cttccctggt ggcatgggct gtgtgaccct cctgccagag aaattccgag gtcctcagga
     2161 aaagcctgga tcttttctcc cacctcatca cgttgcacca ctggttgata aggatgggca
     2221 ggtccctcca ggactggacc cacttgctgt acatgtgacc gatcaccgtc tcggaggtag
     2281 ggcggacaat aaggggctct tccagttcct cgcctccggc atgggtcacc acggcacact
     2341 ccggggcaaa gccctcaaca tgctcggctt ccttttcaag gaaggacatc gggatgagaa
     2401 gcgggaaata ggcgttgaca tggcccgtat ccttgaaggc ctcgtcaaag tgcttctgga
     2461 taaactccca gagggcgtag ccgtccggtc ggatgatcat gcagcccctt acaggggcat
     2521 agtcggccag ttcggctgcc ttgatcacgt cgaggtacca ctgggaatag tccttctccc
     2581 ttgaaacgat attccgtgcc atatccattt cctcctcatc ctgtcgcgtc ttcctgacgt
     2641 aattccaaat ataccgcaca ttgtaggcat aaaaagtgta aggggcaagg ctgaaccctc
     2701 tcccccagct ccaggggtca gggcaacgga tcggtgtccc agaagggcct gccgatggtg
     2761 aaaccgaagg tccactcgga cctgtcgtcg taaatgacga gcagcctgcc gttgaggaac
     2821 cttcctggaa gggagagagc cagccccgcc tcccaggcgc tctcaaggcg ctcccacgaa
     2881 ggaccgtagg actgccccac gttggcaatg atatcaaggt tgaccgtacc ccaccagctt
     2941 ctctggaaaa ccctcctgaa ggccatgcct ccccatgctg cgttctcccc cgcaagaggg
     3001 cgagaggcca gggaaaggag ctcctccctt gtccccaggt aaaccgaatg gtatggacgc
     3061 gaagcgtccc ccgccgttgc gccacccttg acaagaagcc tccagcgctc acccagggga
     3121 gtcacccaga gtagatccat cctggcaaga agggaggagg tatccatcca ccaggccgac
     3181 agcgacgccg agaaaccttc ggcgggatcg aaggggtcgt caaggccgtc ccatgcaagg
     3241 gtcaacaggg gaccgtggtg ggtacgttct tcaccgtcaa aatggatgaa ctcaccggca
     3301 taccccagag aagccctgaa ttgccctcgg gagttgatac ccgacagggc cagggagta
//
LOCUS       DAOPKQ010000155         3330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_32132_length_3330_cov_5.748702, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000155 DAOPKQ010000000
VERSION     DAOPKQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32132_length_3330_cov_5.748702"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="buk"
                     /locus_tag="PK146_05785"
     CDS             complement(<1..1011)
                     /gene="buk"
                     /locus_tag="PK146_05785"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPE91787.1"
                     /translation="MKSAVILAINPGSTSTKVALYRGSEEVWSDTQRYDADRIEEFPD
                     IPAQEQFRLEEIRKALKGKNTELTGLDAVVGRGGLLRPIPGGTYRVSQEMLEDLRSCR
                     YGSHASNLGGPLAKMIADEAGGLPAFIVDPVVVDELAEEARLSGIPEIERRSIFHALN
                     QKAVARRASAGLGKSYEEVNLIVAHMGGGISVGAHCRGRVIDVNNALDGEGPFAPERA
                     GSLPAGELVKFAFSSGLQLEGLIKKMVGGGGLVAHLGTNDLREVEKRIARGDEMASLV
                     FRAMAYQVAREIGSRAAILSGDVDAVVLTGGLAFSEQFVKDISQRVAFMAPVMVFPGE
                     DEM"
     gene            complement(1030..1950)
                     /locus_tag="PK146_05790"
     CDS             complement(1030..1950)
                     /locus_tag="PK146_05790"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HPE91788.1"
                     /translation="MKRITSLVELLAYAKEIGPKKIAVALAEDAEVMEAVENARKEGV
                     ADAFLVGDADKLKKVTEPMGIDLSNYEVVDVKGGQGEIGIEAVKLVSSGKADILMKGM
                     ISTANFLRGVLNKEVGLRSGGTLSHVYIHQVEGYDRIFFICDPAFNIAPDFQTKVNLI
                     NNTVNLAHAFGIDTPKVAALAAVEVVNQDMPCTIDAAMLTQMNRRGQIKGCIIDGPLA
                     LDNAISEESAKHKGIVSEVAGHADILHVPDIEAGNMLAKAIVYFANNKTAGLVLGAKA
                     PVVLTSRSDSPETKLLSIASAVVLSAYEKK"
     gene            complement(2102..3007)
                     /locus_tag="PK146_05795"
     CDS             complement(2102..3007)
                     /locus_tag="PK146_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPE91789.1"
                     /translation="MSFETLDFLLERSQSGKTMVLAVAAPHGEDILGAVAEAKERGVV
                     SPILYGDRSRIEKIASELSIDVSSFNMVEEPDEGRSAELAVKAVSSGEADLLMKGNVK
                     TATLLKAVLNKEWGLRSGSLLSHVFLFQIPKIGKVFCMTDGGMSMYPDLAAKQGIIEN
                     AVECYHKLGIQCPRVALLAAVEVVNQDMPCTIDAAALTQMNRRGQIKGCIVDGPLALD
                     NAVSEESARHKGIVSEVAGKADILVVPDIEAGNLMGKVMLYMSGGRGAGVIVGARKPI
                     VLTSRFDNAETKLLSIAFGAVLAKA"
     gene            complement(3012..>3330)
                     /locus_tag="PK146_05800"
     CDS             complement(3012..>3330)
                     /locus_tag="PK146_05800"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163831.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPE91790.1"
                     /translation="EVSSRVDSGDEKAALVYRAMALQISKEIASFAATLSGKVDAVIL
                     TGGIAHDVSFVGLITSRIQWIAPVLVYPGEDEMRALAQGALRVLSGQEEALVYRDSVL
                     RRA"
BASE COUNT          641 a         1045 c          922 g          722 t
ORIGIN      
        1 catctcgtcc tctcccggga ataccattac cggagccatg aaggccactc tttgagagat
       61 atcctttacg aactgttcgc tgaaggccag ccctccggtg aggaccacgg catccacatc
      121 accggagagg atcgccgccc ttgaccctat ctcacgggcg acctggtagg ccatagccct
      181 gaaaacgaga ctggccattt catccccccg ggcgatcctc ttttcgacct cccggaggtc
      241 attggtaccg agatgggcca caaggccacc tccacctacc atcttcttga taagcccttc
      301 tagctgtaac cccgatgaaa aggcgaactt gacaagctct cccgcgggaa gggaacctgc
      361 cctctcggga gcgaagggcc cctctccgtc gagggcgttg ttgacatcga tcactcttcc
      421 cctgcagtgg gcccctacgg atatccctcc ccccatatgg gctactatga ggttcacctc
      481 ttcgtagctt ttgccaagtc cggctgaggc tctccgggcc acggctttct ggttgagggc
      541 gtgaaagatc gaccttctct cgatctcagg gatcccggaa aggcgggcct cctccgccag
      601 ttcatcgacc accacagggt caactatgaa ggcggggagg ccgccggcct cgtcggcgat
      661 catctttgcc agcggacccc cgagattgct tgcgtgggag ccataccggc agctcctgag
      721 atcctcgagc atctcctggc tgaccctgta agtcccgccg gggatggggc gaaggagacc
      781 gccccttccc acaactgcgt caagaccagt cagctctgta tttttcccct tcagcgcctt
      841 gcggatctct tcaagcctga actgctcctg ggcagggatg tcaggaaact cctctatccg
      901 gtctgcatcg tacctctggg tatccgacca gacctcctcg gaacccctgt agagcgccac
      961 ctttgtgctg gtggagccgg ggttgatggc gagaatgacg gcggatttca tccgggaccc
     1021 cggcaggagc tacttcttct cgtaggccga gaggacgacc gcggaagcaa tagagagcag
     1081 tttcgtctcg ggcgaatcgg acctgctggt gagcaccacg ggcgccttgg ctccgaggac
     1141 cagcccggcc gtcttgttat tggcgaagta gactatggcc ttggcgagca tgttccctgc
     1201 ctcgatgtcc gggacgtgga ggatgtcggc atgaccggcc acctcggaga ctatcccctt
     1261 gtgcttcgca gactcttcgc tgatggcgtt gtccagcgcc aggggtccgt cgatgatgca
     1321 ccccttgatc tggcccctgc ggttcatctg ggtcagcatg gccgcatcga tggtgcaggg
     1381 catgtcctgg ttgaccactt cgaccgccgc gagagccgcc accttcggcg tgtcgatccc
     1441 gaaggcgtgg gcaaggttca cggtattgtt gatcaggttg accttggtct ggaagtcggg
     1501 agctatatta aaggccggat cgcagataaa gaaaatgcga tcgtagccct caacctggtg
     1561 gatgtagacg tgggataggg tccctccgct gcggagaccg acctccttgt taaggacccc
     1621 tctcaggaag ttggccgtgg agatcatgcc cttcatgaga atatccgcct taccggagga
     1681 gaccagcttg acggcctcga tacctatctc tccctgtccg ccctttacat ccaccacttc
     1741 gtagttcgaa aggtcaatac ccatcggctc ggtcactttc ttgagcttgt cggcatcacc
     1801 gaccaggaac gcatcggcaa ccccttcctt gcgtgcgttc tcgacagcct ccatgacctc
     1861 ggcgtcctcc gccagggcga cggctatctt cttgggaccg atctccttgg cgtaggcgag
     1921 aagttcgacc agtgacgtga tccttttcat caacgaaacc tcccaacgtg aaatttataa
     1981 cgttcatttt acatttaaac gccctaagta aaaacttcga aggctacagg agtcaggcat
     2041 caggaggcag gaaaaccggt taccgggacc gagggtttgc ctgtcgcctg ccgccttgtt
     2101 tctatgcttt cgccagcacc gccccgaagg ctatggagag cagcttggtc tcggcgttat
     2161 caaagcggct ggtcaggacg atgggcttcc gggcgcccac aatgacccct gcgcctcgtc
     2221 cgcccgacat gtagagcatg accttgccca taaggttgcc ggcctcgatg tcggggacca
     2281 ccaggatgtc cgccttgccg gccacttccg atacaatccc cttgtgtctt gcagactcct
     2341 cgctcacggc gttgtccaga gcaaggggcc cgtcaacgat gcaccccttt atctgccccc
     2401 ggcggttcat ctgggtcagc gcagcggcat cgatggtgca gggcatgtcc tggttcacca
     2461 cctccactgc ggccagaagc gcgaccctgg gacactggat gccgagcttg tggtagcact
     2521 ccactgcatt ctcaattatc ccctgcttgg ctgccaggtc ggggtacatg gacatccccc
     2581 cgtcggtcat gcagaagact ttcccgatct tgggtatctg gaagaggaag acgtgggaga
     2641 ggagggaacc ggagcgaagc ccccactcct tgttcaggac agccttcaaa agggtggccg
     2701 tcttgacgtt tcccttcata aggaggtcgg cctcccccga tgaaacggcc ttgacggcca
     2761 gctcggcgga acggccttcg tcgggttcct ccaccatgtt gaaggaggaa acatctatgg
     2821 agagttcaga ggctatcttt tcgatcctgc tcctgtctcc gtagaggatg ggcgatacga
     2881 cccccctctc cttcgcttcc gccacggccc cgaggatgtc ctcaccgtga ggcgctgcta
     2941 cagcgaggac catggtcttg cccgactggc ttctttcaag cagaaaatca agggtttcga
     3001 acgacattac atcatgccct ccttaaaacg ctgtccctgt agaccagggc ctcttcctgt
     3061 cccgagagga ccctcaaagc tccctgggca agggccctca tctcgtcctc gcccggatag
     3121 accagaacag gtgctatcca ctggatccgg gaggtgatga gcccgacaaa agagacatcg
     3181 tgagctatcc ctccggtgag gatcactgca tcgacctttc ctgaaagggt tgccgcgaag
     3241 gatgcgatct cctttgatat ctgaagtgcc atggccctgt agaccagggc cgccttttca
     3301 tcgccggaat cgactctgga ggatacctcc
//
LOCUS       DAOPKQ010000156         3310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_32373_length_3310_cov_5.516129, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000156 DAOPKQ010000000
VERSION     DAOPKQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32373_length_3310_cov_5.516129"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK146_05805"
     CDS             <1..182
                     /locus_tag="PK146_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91791.1"
                     /translation="TGEHERFIELLETGDAKAASDYMRDVHWSFEVQKPWIRRYYSEL
                     EKERNLEGIRAEVIR"
     gene            179..1093
                     /locus_tag="PK146_05810"
     CDS             179..1093
                     /locus_tag="PK146_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPE91792.1"
                     /translation="MNGNTRGIIFDIKRYSIHDGPGIRTTIFLKGCPLSCWWCHNPES
                     QSAFAEIIYRGDRCIGCGACVTACPAGAISLTQRGYVADPDRCAGCGECVRACPAEAR
                     EMIGREVGVEEILKEIEKDLLFFDESGGGVTFSGGEPLSQPVFLVEVLRECCRRDIHT
                     VVDTCGFATLDVMKQVAPYTRLFLFDLKMMDTSLHRKYTGVPNEVIIENFRWLMSEKH
                     NVRVRIPVIPGINDSLENMEATGEFLSGFPSRPEVNLLPYHGSATEKYHRFSIPYRMA
                     DVSAPSGERMKELSDQLARFGLAVSTGG"
     gene            1099..>3310
                     /locus_tag="PK146_05815"
     CDS             1099..>3310
                     /locus_tag="PK146_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HPE91793.1"
                     /translation="MNERIERLRTESFEAPVTLDHERAGIVTDFYIENFGKNSVPVTR
                     ALAFKALCEKKSIYIGKDELVVGERGPFPKSVSTYPELNCHSLEDLEILDTREKNPYL
                     VPQKCREVYRDKVIPYWRGRTMRDKIFADIGEEWKETYAAGYFTEFMEQRAPGHTTLD
                     GKFYSKGMEDFKKEIAEAIACLDFKIDPEATKKREQLKAMDITCDAVIIFAERHAELA
                     KKMAAGEGDPERRKELEKIASNCMNVPRRAPRDFWEALQMYWFMHLGTITELNGWDAM
                     NPGHLDRYFDPFYQEEIKAGTITPEWAKELLSAFWIKFNNHPAPPKVGVTAAESGTYN
                     DFTNINLGGLLPDGTDGVNDISYMMLEVMDEIHLLQPQANIQLSRKNPDRFLKAALRV
                     IRKGYGYPSVFNADTVIEQMIRAGKTVEDAREGGTSGCVETGAFGKEAYILTGYLNTP
                     KIFELALNNGVDPVSGKKIGVETGDPAGFKSFEDLYAAFEKQLHHIVDIKVRGNQFIE
                     RMYADYAPAVFLSVLTDDCIKNGKDYNDGGPRYNTNYIQCVGIGTMTDSLSALKKHVF
                     EEKTFEMSEVVASVGKNFDGSEAMRLTFANKTSRWGNDDDYADDLMKRVFNSLFDEID
                     GRRNTKGGEYHIDMLPTTCHVYFGSITGATPDGRLAGTPLSEGISPVQGADRNGPTAV
                     IKSCGKMDQIRTGGTLLNMKFMPKVLEGEEGIDKLAKLVRTYFRYDAHHIQFNVV"
BASE COUNT          822 a          885 c          944 g          659 t
ORIGIN      
        1 ccaccggcga acacgaacgt ttcatcgaac ttctggagac gggggatgca aaggctgcct
       61 cggactatat gcgagatgtt cactggtcct tcgaggtcca gaaaccctgg attcgcagat
      121 actacagcga gctcgaaaaa gaaaggaacc tcgaggggat ccgtgcggag gttattagat
      181 gaacggtaat accaggggca tcatcttcga cataaagcgc tattcaatac acgacgggcc
      241 cggaataagg accaccatat tcctgaaagg atgtcccctg tcgtgctggt ggtgccataa
      301 tccagagagc cagtcggcgt ttgccgagat tatctaccgt ggcgaccgct gcataggctg
      361 tggagcctgt gtgacggcgt gccctgcggg tgccatctcg ttgacgcagc gcggttacgt
      421 tgccgatccc gaccgctgcg caggctgcgg cgagtgtgta cgggcctgcc ccgccgaggc
      481 gagggagatg atcggccggg aggtcggcgt cgaagagatc cttaaggaga tagaaaagga
      541 tctcctcttt ttcgacgagt ccggtggtgg agtcaccttt tcaggcgggg aacctttgtc
      601 ccagcccgtt tttcttgtgg aggtcctcag ggaatgttgc aggagggaca tccacactgt
      661 tgtggatacc tgcgggtttg ccacgctgga tgttatgaag caggttgccc cttatacccg
      721 gcttttcctc tttgacctca agatgatgga cacttcactt cacaggaagt acacaggtgt
      781 tccaaacgag gttatcattg agaattttcg ttggcttatg agcgaaaagc acaatgtaag
      841 agttcggata ccagttattc cgggaataaa tgacagcctt gagaacatgg aggccacagg
      901 tgagttcctg tcgggtttcc cttcgagacc ggaagtaaac ctgttgccct accacggctc
      961 ggcgacagaa aaataccacc ggttcagtat tccctaccgc atggccgatg tttccgctcc
     1021 ctcgggggaa cggatgaaag aactttccga ccagcttgcc cggttcgggc ttgccgtgtc
     1081 tacaggagga tgagagaaat gaatgagagg atcgagagac tccgtaccga gagtttcgag
     1141 gcgcccgtta ctcttgacca tgagagggcc gggatagtca ccgatttcta tatcgaaaac
     1201 ttcggcaaga actcagtgcc cgtgacccgt gcccttgcct tcaaggccct gtgcgaaaag
     1261 aagagcatat acatagggaa ggatgagctg gtagtcgggg agagggggcc cttccccaag
     1321 agtgtctcca cttacccgga actcaactgt cattcccttg aggacctgga gatactggac
     1381 acaagggaaa agaaccccta cctggttccg cagaagtgca gggaggtcta ccgggacaag
     1441 gttatcccct actggcgcgg ccgcaccatg cgcgacaaga tattcgccga cattggcgag
     1501 gagtggaagg agacctacgc tgccggctat ttcaccgagt tcatggagca aagggccccg
     1561 gggcatacaa ccctggacgg caagttctac tctaaaggca tggaagattt caagaaagaa
     1621 attgccgaag ccattgcctg cctggatttc aagatcgacc ccgaggcgac aaaaaagcgc
     1681 gaacagctca aggccatgga cattacctgc gacgcggtca tcatttttgc cgagcgtcat
     1741 gccgaactcg caaaaaagat ggcagccggt gaaggcgatc ctgaacgcag gaaggaactc
     1801 gagaagatag cctccaactg catgaatgta ccgcgccgtg ctccccggga cttctgggag
     1861 gcccttcaga tgtactggtt catgcatctg gggaccatca cggaacttaa cggctgggat
     1921 gccatgaacc cgggacacct tgaccgctac ttcgacccct tctatcagga ggagatcaag
     1981 gccggcacca tcacgccgga atgggcgaag gaactgctca gcgcattctg gatcaagttc
     2041 aacaaccacc cggcgccccc caaggttgga gttaccgcag ccgagagcgg cacctacaat
     2101 gacttcacca atatcaacct gggcggcctc ctccccgacg gcaccgacgg ggttaatgat
     2161 atttcctata tgatgctcga ggttatggac gagatacacc tcctccagcc ccaggccaac
     2221 atacagctca gccgcaagaa tcccgacagg ttcctcaagg ctgccctcag ggtaataaga
     2281 aagggttacg gctacccgtc ggtgttcaac gccgatacgg tcatagagca gatgatccgc
     2341 gccggaaaga ccgtcgagga cgcccgcgag gggggtacca gcggatgcgt cgagacgggt
     2401 gccttcggca aggaagccta catcctcacc gggtacctca atacccccaa gatttttgag
     2461 ctggctctca acaacggcgt tgatcccgta tcgggcaaga agatcggcgt cgagacgggg
     2521 gatccggccg ggttcaaaag cttcgaggat ctttacgctg ctttcgagaa acagctgcac
     2581 catattgtgg atatcaaggt caggggcaac cagttcatag agcgcatgta cgcagactac
     2641 gccccggcgg ttttcctctc ggttcttacc gacgactgca taaagaacgg caaggactat
     2701 aacgacggag gcccgcgcta taacaccaac tatatccagt gtgtcggcat agggacaatg
     2761 acggacagtc tgagcgccct gaaaaaacat gtcttcgagg agaaaacctt cgaaatgagt
     2821 gaagttgtgg cttcggtagg caaaaacttt gatggcagcg aggccatgag gttgaccttc
     2881 gccaacaaaa catccaggtg gggcaacgat gacgactacg ccgacgacct gatgaagagg
     2941 gttttcaact ccctgttcga cgagatagac ggaagacgca acaccaaggg tggggagtac
     3001 catattgaca tgcttcccac tacctgtcat gtttactttg gatcaataac cggggccacc
     3061 cccgacgggc gtcttgcggg cactccgctt tcagagggga tatcccctgt acagggagcg
     3121 gatcggaacg gaccgacggc ggtcataaaa tcctgtggca agatggacca gatccgtacc
     3181 ggaggaacct tgctgaacat gaagttcatg ccaaaggtcc ttgaggggga ggaagggatc
     3241 gacaagctcg caaaacttgt acgaacctac ttccgttacg acgcgcacca catacagttc
     3301 aacgtcgtag
//
LOCUS       DAOPKQ010000157         3280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_32801_length_3280_cov_4.281550, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000157 DAOPKQ010000000
VERSION     DAOPKQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32801_length_3280_cov_4.281550"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PK146_05820"
     CDS             <1..164
                     /locus_tag="PK146_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPE91794.1"
                     /translation="TSHAPIVCLELRIPCIVSAADAISVLDDGMIVTVDGRRGVVLRG
                     MVRLHSDGK"
     gene            154..783
                     /locus_tag="PK146_05825"
     CDS             154..783
                     /locus_tag="PK146_05825"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPE91795.1"
                     /translation="MANDREGATRLRSILSKIPSSGIDRGWKDLSDSSGRKSAVLVPV
                     FPRDGELFLLLLERSVSLRRHPGQIAFPGGAMEKRDRGPVDTALREFTEETGIPGERV
                     EIVGTLPEVRAYSSDFSLFPVVGLIPSGIGPGDLSPDSHEVKSVLEVPLKALERAPVM
                     ENFTREGKVLSYPVFRIGDGVNIWGATAWIILNLIRTLKDGDIKGSQCP"
     gene            774..1616
                     /locus_tag="PK146_05830"
     CDS             774..1616
                     /locus_tag="PK146_05830"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPE91796.1"
                     /translation="MSLIGTHVSVAGGLWKAIERGEALGCEAIQIFTKNQLQWRAFPI
                     PEPAATRFMAAWAKSSIRKVVAHGSYLLNLASVKETREKSIAALVEEIARCEALGIDD
                     LVLHPGSHLGDGLDNGRKLVAEGLRRAIERTPRMRARVLLETMSGQGHSVGSTIEDLA
                     WILDRVGDISRTGVCLDTCHLFAGGHELRGRSSYDRLLRSVERHIGLGRIGCWHLNDS
                     RTEMGSRRDRHQHIGEGFIGTELFSCIVSDPKWEDTPCLLETPKGGPGDEGNIALLRK
                     MRGH"
     gene            1669..2742
                     /locus_tag="PK146_05835"
     CDS             1669..2742
                     /locus_tag="PK146_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HPE91797.1"
                     /translation="MRYPEICINTGVISKNTETVVKLCADKGIRVWGVTKGVSAHPAV
                     ASAMLEGGCQGLADSRVDNIRKLRLFGFNNPMMLLRIPMPGEMDEVAETADSCLISMP
                     GSVLMLDSACREAGTEVGIIVMVDLGDLREGIWPDGVEEMAEAIRQCRRVRYLGVGSN
                     LGCLSGVLPTEKNLSLLVRTGEELSSFLECPLGVVSGGATSTLMLVEKGIVPEGVNQL
                     RVGEGILLGTDITRSRLIPYLENDAIRITAEVVEVRRKPSMPIGETGTDAFGNVQVFR
                     DRGVRRRAIVAMGRQDVRPEGLTPVDEGLSIIGASSDHLTLDAEDLRGSLEPGDTISF
                     VPDYGAVLAATTSPYVRKRIINS"
     gene            2770..>3280
                     /locus_tag="PK146_05840"
     CDS             2770..>3280
                     /locus_tag="PK146_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPE91798.1"
                     /translation="MLESIRWQDFIDIAIIAFIVYRLLLLLVDTRAMQLVKGLFVIGL
                     LFATARLMKLAALSWILGRMLGVFIIAIPIVFQPEIRRMLEELGRGNIWRRRQAEERA
                     AQLTDEITRALLFLQSRKIGAILVFQKDTGLKDIWRSAVQIGGEISQDLLVSIFWPGN
                     PLHDGATILD"
BASE COUNT          704 a          828 c         1070 g          678 t
ORIGIN      
        1 tgacgagtca tgccccgatc gtatgtcttg aactccggat cccctgcatt gtttccgccg
       61 ccgatgcgat ctccgttctg gacgatggca tgatcgtgac cgttgacggg aggcgcgggg
      121 tggtgctacg ggggatggtc aggctccatt ccgatggcaa atgatcggga aggggccacg
      181 aggctccggt ccattctttc gaaaattccc tcctcaggga tcgacagggg ttggaaggac
      241 ctttcagata gttctggaag gaagagtgcc gtgcttgtcc cagtcttccc gagggatggt
      301 gaactcttcc ttctcctgct ggagcggtca gtttctctga gaagacatcc gggacagata
      361 gcctttcccg ggggtgccat ggagaagagg gaccggggtc cagtggacac agctctcaga
      421 gagttcaccg aggagacggg gatccccgga gagagagtgg agatagtcgg gacactgccg
      481 gaggtcaggg cctattcgag cgatttttcg ctattccctg tggtgggatt gatcccctcc
      541 ggtataggtc cgggtgacct gtcgccggac agtcacgaag tgaagagcgt tctggaggtt
      601 cccctgaagg cactggaacg ggcccccgtc atggagaact tcaccaggga gggcaaggtc
      661 ctgagctacc ctgttttccg gatcggcgac ggagtgaaca tatggggggc gacggcctgg
      721 ataatcctca accttatccg gaccctgaag gacggggata taaaggggtc ccaatgtccc
      781 tgatcggtac ccatgtctcc gttgccgggg gtctgtggaa agcgatcgaa agaggagaag
      841 ccctgggctg cgaggccatc cagatcttta ccaagaacca gctccagtgg cgggcctttc
      901 ccataccgga gccggccgcg acgaggttca tggctgcatg ggcgaagagc agcatcagga
      961 aggtggtcgc ccatgggtcc tatcttctta acctggcgtc ggttaaggaa acccgggaga
     1021 aaagtatcgc ggccctggtg gaagagatcg cccgatgtga agctttgggt atcgatgacc
     1081 tggtactgca cccgggatct cacctggggg atggactgga caatggcaga aaactggtgg
     1141 ccgaaggatt gaggcgggcc atcgagagga caccaagaat gagagccagg gtccttctgg
     1201 agaccatgtc gggccagggc cactccgttg gctcgacaat agaggacctg gcatggatac
     1261 ttgacagggt gggcgatatc tcacgcaccg gggtgtgcct tgatacgtgc cacctcttcg
     1321 ccggaggtca cgagctgcgg gggcgctcgt cctacgaccg gcttctcagg tccgtggaga
     1381 ggcatatcgg actgggaagg atcggatgct ggcacctcaa cgactcccgg acggagatgg
     1441 gaagccggcg ggacaggcat cagcatatcg gggaaggttt cataggcaca gagctcttct
     1501 cctgcattgt ttccgatcca aagtgggagg atactccgtg cctgctcgag accccgaagg
     1561 gcggacccgg tgacgagggg aatattgccc ttctcagaaa aatgaggggc cattgagtga
     1621 gaatgttagt ggaatagaag ccaggaaagc ggggcggtac agttggccat gaggtatccg
     1681 gagatctgca tcaatacggg agttatatcg aaaaacactg aaacagtggt caaactctgt
     1741 gcagacaagg gcatacgcgt ctggggtgtt accaagggtg tcagcgccca tccggctgtg
     1801 gcgtcggcga tgcttgaggg aggttgtcaa gggctggccg acagtcgtgt agacaatatc
     1861 cgaaaactcc gccttttcgg gttcaataat cccatgatgc ttttgaggat cccaatgccg
     1921 ggcgagatgg acgaagtggc ggagacagcc gatagctgcc tgatctccat gccaggctcg
     1981 gtcctgatgc tagacagtgc ctgcagggaa gccgggacag aggttggcat catagttatg
     2041 gtggaccttg gcgatcttcg cgagggtatt tggcccgatg gtgttgagga gatggcagaa
     2101 gcgataagac aatgccgcag ggtccggtat ctgggggtgg ggagcaacct tggatgtctt
     2161 tcgggggtac tccctacgga gaagaacctg tctctcctcg tcaggaccgg ggaagagctt
     2221 tcctcgttcc tggagtgccc gctgggagtc gtatcaggag gcgcgacgtc tacgctgatg
     2281 ctggtggaga agggcattgt tcccgagggg gtcaaccagc tgcgagtggg tgaaggtata
     2341 cttctgggga cagatataac ccggagcagg cttataccat acctggagaa cgacgcaata
     2401 aggataacgg ccgaggtggt agaggtcagg aggaaaccct ccatgccgat cggagagacc
     2461 gggaccgatg ccttcgggaa cgtccaggtc ttcagggaca ggggagtgag aaggcgggcc
     2521 atagtcgcaa tggggaggca ggatgtcaga cccgaggggc ttacccctgt tgatgagggg
     2581 ctgtccatta tcggagcctc cagcgatcat ctgactctcg acgcagaaga tctcaggggt
     2641 tcccttgaac cgggtgatac tatatccttc gttccggatt acggcgccgt tctggcggcc
     2701 acaacttcgc cctatgtcag gaaaaggatc attaacagct gaactgtcag gagtgatagc
     2761 ttgtccggga tccttgagtc catacgctgg caggatttca ttgatatcgc gataatcgca
     2821 tttattgtct acaggctgct tctcctgctt gtggataccc gtgccatgca gcttgttaag
     2881 gggcttttcg tcatcggcct cctcttcgca accgcacggc tgatgaagct tgccgcactc
     2941 tcatggatcc tgggaaggat gctgggggtc ttcatcatag ccatccctat cgtcttccag
     3001 cccgagataa ggaggatgct cgaggagctc ggacggggca acatctggag gcgccgtcag
     3061 gcagaggaga gggcggccca gctgaccgac gagataaccc gggctctcct cttccttcag
     3121 agccgtaaga taggagctat cctggtcttc cagaaggaca caggcctgaa ggatatatgg
     3181 agatccgccg tccagatagg gggagagata tcccaggatc ttctggtctc catcttctgg
     3241 ccggggaacc ccctccacga cggtgcgacc attcttgaca
//
LOCUS       DAOPKQ010000158         3279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_32817_length_3279_cov_3.301799, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000158 DAOPKQ010000000
VERSION     DAOPKQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32817_length_3279_cov_3.301799"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..281
                     /locus_tag="PK146_05845"
                     /pseudo
     CDS             60..281
                     /locus_tag="PK146_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
     gene            648..986
                     /locus_tag="PK146_05850"
                     /pseudo
     CDS             648..986
                     /locus_tag="PK146_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662414.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
     gene            1001..2530
                     /gene="purH"
                     /locus_tag="PK146_05855"
     CDS             1001..2530
                     /gene="purH"
                     /locus_tag="PK146_05855"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPE91799.1"
                     /translation="MGQKRALISVFDKSNVAGLARDLADLGWEIVSSSGTARHIRESG
                     VPVKEVEDLTGYPHMLGGRVKTLHPSVFGGILARRHFEGDLIDVEKFGIPMIDMVVCN
                     LYPFEETFNQGADLDELLENIDIGGVTLIRAAAKNFRHVIPVTDPEDYGVVAEEIRSE
                     GNVSLPTRERLALKAFLKTACYDSVIHEGLSRSCGSERESFPSLTVLPLSRKIELRYG
                     ENPHQRASLFTHPLEKTPWRLLSGKPLSYNNILDLDCAMRSMALFQSDCACIIIKHTT
                     PCGIAVADDAASAYLRAFECDPVSAFGGIIAFTREMDGNAARRVLDQFAEVIVAPSFS
                     EEAALILVSERPNLRLLEWNGGKVAGPSFVSTWSGTLMQDDVLPPLPDHGSGEWVGQP
                     RPDLWEDLVLAWKAAALSKSNSISLVREGSTVGLGRGFTSRVDAVRWACIQADGKAVG
                     AVMGSDAFFPFPDSLEIAADAGVAAVIQPGGSIRDEEVRGAAMRLGISMFISGCRTFR
                     H"
     gene            2545..>3279
                     /locus_tag="PK146_05860"
     CDS             2545..>3279
                     /locus_tag="PK146_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPE91800.1"
                     /translation="MRVLVLGGGGREHALCWAFAASPSVSSVHCAPGNAGIEKTTTVH
                     PVDISDPGTVLELAGTLSPDLVVIGPEAPLVAGAADVLRDRGFDVLGPGKEGAMLEGS
                     KAFAKNFMSRHGVPTAGFDICENLEQAEKALLKRKPPYVVKADGLAAGKGAFVIDSFM
                     EAMETCAGLLVKMFLGRSGKRVVIEDHLPGEELTVLVLTDGVSFRILPTSQDHKRAFD
                     ADKKPNTGKMGAYSPVPWADKPFMDRV"
BASE COUNT          739 a          864 c          940 g          736 t
ORIGIN      
        1 attaccctga tctcagcagt aagcagattc ttgtaatgga atggattgat gggtattcca
       61 tgctcgacct tattcgcatg aaacgcaagg aagaactgcc tgattttgat ttttctctcg
      121 aggaaaaatt tgaagaaatt atcagggatg ttgcgatcaa atcgttgctc cgaggatatt
      181 ttcacgcgga tgcgcacccg gcgaacatca tgatcaccaa agagggcaag attgctctta
      241 tcgattttgg tttgatacaa tttttcagca gagaggtgag gaagggaaca attttgtttt
      301 tgctcggcat tacgtcaaac gacatcgaac tcatttttaa atcagccgaa atgcttggta
      361 aagaaaacgc gcaatttaat cgtgacgaag tgtacgaaga aataagccgt cacctctacg
      421 attaccttga tgcttcagct catgatgtca gctcgaccaa aattttgttt agtattttaa
      481 aggtgtgcct tgagcagggc tttcaatatc catggagcct tgtcctctat acgcgtacgg
      541 ccgtaaacct cgacgggcaa attttgagag tccaccctgg cttctcattc tcgtcatacg
      601 gaaggcaata cttgcttgag gtatatctcg agacgctgct cgaagcccat tccgtagaat
      661 ggctggtcct tgccggtttc atgaggatcc tatcaccggg attcgttgcc cccaggaggg
      721 ggaggatcgt caatatccac ccttccctcc tcccatcctt cccaggccgg gactctatca
      781 gtgaggcttt cgcgcacggc gttaaattga cgggcgtcac cgtgcacctc gttgacgaag
      841 aagtggacca tggagagata cttgcccagg agcctgtcag gatactccct ggtgatacac
      901 ttgaggaact ggaggagagg atccaccagg cagagcatgt catgtataca agaaccctga
      961 tcgccctgct tggccgatca ggatgaaagg agaacttgcc atgggtcaga agcgtgcgct
     1021 gatatcggtc tttgacaaga gcaacgtggc cgggttggcc agggatcttg cggacctggg
     1081 gtgggagata gtatccagtt cgggtaccgc caggcacatc agggagtcag gggttcccgt
     1141 caaggaagtt gaggacctta ctggataccc ccacatgctg ggaggaaggg tcaagaccct
     1201 ccacccctct gttttcggcg ggatccttgc caggagacac tttgagggtg acctgatcga
     1261 cgtggaaaaa ttcgggatcc cgatgatcga catggtggtt tgcaacctct accccttcga
     1321 agagaccttc aaccagggtg ccgatctgga cgaacttctc gagaacatcg acatcggggg
     1381 cgtcaccctc ataagggccg ccgccaagaa tttcagacac gtaatacccg taactgaccc
     1441 tgaagactac ggtgtagtgg cggaggagat ccggtccgag ggcaatgtct ctctccccac
     1501 cagggagaga ctggccctta aggctttcct gaagacagcc tgctacgatt cggtcattca
     1561 cgagggcctc tcgagatcct gcggttcgga aagggagtct ttcccttctc tcactgtact
     1621 tccgctaagc aggaagatag aactccgata cggggagaac cctcaccaaa gagcctctct
     1681 ctttactcac cctcttgaaa agaccccctg gagacttctc tccggaaagc ccctgtccta
     1741 caacaatatc ctcgatctgg actgcgccat gagaagtatg gcccttttcc agtctgactg
     1801 tgcctgcatt atcatcaaac acaccacgcc ctgcggtatc gccgtggcag acgatgccgc
     1861 ctcagcatat ctgagggcct tcgaatgcga tcccgtatcg gcctttggcg ggataatcgc
     1921 ctttaccagg gagatggacg gtaatgccgc gcgacgagta ttggaccagt tcgccgaagt
     1981 catagtggcg ccgtccttct cggaggaggc agcactgata ctggtctcgg aaagacctaa
     2041 tctcagactc cttgagtgga acggagggaa ggtcgccggg ccctccttcg tcagcacctg
     2101 gagcgggacc ctgatgcagg atgatgtcct tcccccgctg cctgaccacg gatccgggga
     2161 atgggtggga caaccaagac ccgacctctg ggaagacctg gtccttgcct ggaaagcggc
     2221 agccttgagc aagagcaact ccatatcact tgtgagggaa ggttctaccg tcggtctcgg
     2281 aaggggtttc accagcaggg tcgacgcggt gagatgggcc tgcattcagg ctgatgggaa
     2341 ggccgttgga gcagtaatgg gttccgatgc ttttttcccc tttcctgaca gtctggagat
     2401 agccgccgat gcgggagtag cggcggtgat ccagcctgga ggatcgataa gggacgaaga
     2461 ggtcagggga gctgcaatga ggcttggtat cagcatgttc atcagtggct gtcggacctt
     2521 ccgccactga acgggggtga gaccatgaga gttcttgtcc tgggcggtgg aggccgggaa
     2581 cacgccctgt gctgggcctt cgccgcatcc ccctcggtga gctccgtcca ttgtgctccc
     2641 ggtaacgcag gaatcgagaa gaccaccact gtccacccgg ttgatatttc ggatccgggg
     2701 acggtcctcg aacttgcagg caccctgtca cccgaccttg tcgtcattgg gcctgaggcc
     2761 ccgctcgtgg ccggtgctgc cgacgttctg cgtgacaggg ggtttgacgt tcttggacca
     2821 gggaaagagg gagccatgct ggaggggagc aaggcctttg cgaagaactt catgtccagg
     2881 cacggtgtgc ccacagcagg tttcgatatc tgcgagaacc ttgagcaggc ggaaaaggcc
     2941 ctcctgaaga gaaaacctcc ctacgtggta aaggccgacg gccttgcagc cggcaagggg
     3001 gccttcgtga tcgactcttt catggaggcg atggagacct gcgctggcct tctcgtcaag
     3061 atgtttttgg gacgatccgg gaaaagggtg gttattgagg atcatctccc cggtgaggag
     3121 cttaccgttc tggtgttgac cgacggggtc tccttcagga tccttccgac aagccaggac
     3181 cacaagaggg catttgatgc cgacaaaaag cccaataccg gaaaaatggg agcctattca
     3241 cccgtcccgt gggcggacaa gcctttcatg gacagggtg
//
LOCUS       DAOPKQ010000159         3265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_33000_length_3265_cov_3.255763, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000159 DAOPKQ010000000
VERSION     DAOPKQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33000_length_3265_cov_3.255763"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /locus_tag="PK146_05865"
     CDS             <1..1895
                     /locus_tag="PK146_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPE91801.1"
                     /translation="NLDLLSALSILEDRFHPLDELEEVDLLALEGYPPIKMGVDVLSR
                     RNLPQYPASAVDGFAVKAAGTVNSSLRTPARISAGSYCWVNTGGGVDRSFDSVVMIED
                     TLMDGDTLLVTRTVTKGENIRPEGEDVTKGQVIARKGDHLDAFLIPLLIASGNSKISV
                     QRLPRSVFIPTGDEIIPAEEWVKEDGQSWDRVPETNSFMLKRLFHDWGFPLKIHGLVR
                     DDETLIEDAVRTALMDHDLVIIGAGTAKGKRDHSADVIGKFSEPLFRGLRMKPGRPVI
                     VGSSGNRPLVALPGFPMSALVICWSVIHPLLRILNGENPAVDLQKAVGAVETLKTSLL
                     MHHSSSQGVMEWIRVKCGDIGGTIYSWPLASGASSTFSTSDADGFALIGSSVLEMPKG
                     SEITVWTKRKIKLEKRAVYQGSNDPAIERIVSYMRERGGDLAVRSVGSLGGLSALARG
                     EGHVASCHLLDPETGVYNDPFIGRLDKTRSWRRFKLFTREQGFLVRKGNPERISSVRD
                     LADKGSIFVNRQPGAGTRVLFDQLLKEAGVQAGSIVGYDNIAITHFEAAARVSGGSAA
                     VTLGVRAAAEAFDTDFIPVTEEDFELVIPEEFISHPGIEILLETLNDGRWRREVDSLG
                     GYRWAF"
     gene            1883..2860
                     /gene="moaA"
                     /locus_tag="PK146_05870"
     CDS             1883..2860
                     /gene="moaA"
                     /locus_tag="PK146_05870"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPE91802.1"
                     /translation="MGVLKDRFGRHLNYIRISVTDRCNYRCLYCMPAEGLEWIPHERI
                     MTYEEILFLARVLKDLGVEKIRFTGGEPLVRKGLFPFLGSLRGFVPGVRTVLTTNGSL
                     IPQYIDEVARSGLSGVNVSLDTLDEGLFRFMTRNGNLQDVIEGIRLLAERTDIPLKIN
                     TVLMKGTNDGEVETLLDFARTHGAVLRLIEFMPLDDSVWRKDLFIPASEILQRLPERG
                     FWERMDNSSDHSAGPARYFRNVSTGQRIGVIEAVSHHFCQSCNRLRVSATGVMRPCLF
                     SEEGIDLRPALLSGNAASVEALVLQAVNSKPQSYCHANTGATHMSRIGG"
     gene            2863..>3265
                     /gene="moaC"
                     /locus_tag="PK146_05875"
     CDS             2863..>3265
                     /gene="moaC"
                     /locus_tag="PK146_05875"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPE91803.1"
                     /translation="MSVFSHLDDSGRPVMVDVGKKKPTLREAEAEALVRLSRKVLDSM
                     IEGKNAKGDVLRVAEIAGIMGAKRTPELVPLCHSIRLDHVSVVCELDEIEATARVRCT
                     ARGTEVTGVEMEAMTGVSVAALTLYDMCKGID"
BASE COUNT          751 a          825 c          987 g          702 t
ORIGIN      
        1 agaacctgga tcttctgtcg gccttgtcca ttctggagga tagattccac cctcttgacg
       61 aacttgagga ggtggatctt cttgccctcg aagggtatcc tccgataaaa atgggtgtcg
      121 atgtgctctc aagaaggaat ctcccccagt acccggcttc tgctgttgac gggtttgccg
      181 tcaaggcagc tggcacggtg aattcctccc tcaggacacc ggccagaata tccgcaggga
      241 gttactgctg ggtcaatacc ggaggcgggg tggaccgctc ctttgactcg gtggtcatga
      301 tagaggatac cctgatggat ggagataccc ttctggtaac aagaacagtt accaagggtg
      361 agaacataag accagaggga gaagatgtta ccaagggcca ggtgatcgcg agaaagggcg
      421 atcaccttga tgcattcctg ataccccttt tgatagcttc cggcaactca aagatcagcg
      481 ttcagagact gccaaggtcg gtctttatcc cgacagggga tgagatcatt cccgccgaag
      541 agtgggtcaa ggaagacggc cagagctggg acagggtccc ggagacaaac tccttcatgc
      601 tgaaaaggct cttccacgac tggggatttc ccctgaagat ccacggactg gtccgtgacg
      661 atgagacact gatagaggat gccgtcagga cggctctcat ggatcacgat cttgtgatca
      721 tcggagcggg aactgccaag ggcaagaggg atcacagcgc cgatgtgata gggaaattct
      781 cggaacctct cttcaggggc ctaagaatga agcccggaag gccagttatt gtgggatcgt
      841 caggaaacag gcctctggtg gcactgccgg gttttccgat gtcagccctt gtgatctgct
      901 ggagcgtcat acaccccctt ttgaggatac tcaacggcga gaaccccgct gttgacctgc
      961 agaaggccgt cggggcagtc gagaccctca agaccagcct tttgatgcat cactcctctt
     1021 ctcagggagt aatggagtgg attagggtca agtgcggtga tattggcggc accatctatt
     1081 cctggcctct tgcctccggg gccagttcca ccttttccac atccgatgca gacggatttg
     1141 cccttatcgg ctcatcggtg ctggaaatgc ccaaaggtag cgaaataacg gtctggacga
     1201 agaggaaaat aaaattggag aagagggccg tctaccaggg ttccaatgac cccgccatag
     1261 agaggatcgt ctcctacatg agagagaggg gcggggacct ggctgtcagg tccgtaggca
     1321 gcctcggggg cctttcggcc cttgccaggg gggagggtca tgttgcctcc tgccacctcc
     1381 tggaccccga gaccggggtt tataacgatc cttttatcgg caggctggac aagacccgga
     1441 gctggcgcag gttcaaactg ttcacaaggg aacaggggtt tttggtcagg aagggcaacc
     1501 cggaaaggat aagctccgtc agggacctcg cagacaaggg gtcaatattc gtcaacagac
     1561 agcccggcgc tgggaccagg gtcctgttcg atcaactgct gaaggaggcc ggggtccagg
     1621 caggatcgat agtggggtac gacaacattg ccatcaccca tttcgaagca gcagcaaggg
     1681 tatcgggcgg ttccgccgcc gttaccctgg gggtcagggc tgctgcggag gctttcgata
     1741 ccgatttcat tcccgtgacc gaagaggatt tcgagctggt cattcccgag gagttcatct
     1801 cccaccccgg catcgagatc ctgttggaga cgctcaacga cggaagatgg aggcgggaag
     1861 tggattctct gggggggtac cgatgggcgt tctgaaggac cgtttcggaa gacatctcaa
     1921 ctacataagg atctccgtaa cagacaggtg caattatcgt tgtctctatt gcatgcctgc
     1981 agaaggactg gagtggatcc ctcatgaaag gataatgacc tacgaggaga tcctttttct
     2041 tgcaagggtc ctgaaagacc ttggtgtaga aaagatccgt ttcaccggtg gggaaccact
     2101 ggtcaggaaa gggctcttcc cctttctggg atcccttcgg ggttttgttc cgggggtacg
     2161 gacggtccta accacaaatg gaagtcttat cccccagtac atcgacgagg tcgcccggtc
     2221 agggctttcc ggggtgaacg tcagcctcga cactctggac gagggccttt tcaggttcat
     2281 gacgaggaac ggaaaccttc aggacgttat tgaaggcatc cggctcctgg ctgaaagaac
     2341 tgacatccct ctcaagatta atactgttct tatgaagggg accaatgatg gagaggtgga
     2401 gaccctcctt gattttgccc gaacccacgg tgccgtcctc cggctgatcg aattcatgcc
     2461 cctcgacgat tcggtctgga ggaaggacct cttcatcccg gcttcggaga tactccagag
     2521 acttccggaa cggggttttt gggagaggat ggacaactcc tcggaccata gcgcagggcc
     2581 tgcccgttac tttcggaacg tatccacggg gcagagaata ggcgtgatag aggcggtctc
     2641 tcaccacttc tgccagagtt gcaacagact ccgcgtctcg gctacggggg taatgagacc
     2701 atgcctgttc tcggaggagg ggatcgatct cagacctgcc cttttatccg gtaatgccgc
     2761 ctcggtggag gcccttgttc ttcaggcggt gaacagcaag cctcaatcct actgccatgc
     2821 caatacagga gcgacccata tgtcgcggat agggggatga cgatgagtgt cttttcacac
     2881 cttgacgact ccggaaggcc tgtgatggta gacgtgggca aaaagaagcc caccctgagg
     2941 gaagctgaag ccgaggcttt ggtcaggctg tcccgaaaag tgctcgattc catgatcgag
     3001 ggcaagaatg ccaagggaga cgtcttgaga gtagcagaga tagcagggat aatgggtgcc
     3061 aaaagaacac cggaactggt gcctttgtgc cattcaataa ggcttgacca tgtctcggtg
     3121 gtctgcgagc ttgacgagat cgaagctact gcaagggtca gatgcacggc gagaggcacg
     3181 gaggtcactg gtgttgaaat ggaagctatg acgggagtat cagtggcggc cctgaccctt
     3241 tatgatatgt gcaagggtat tgaca
//
LOCUS       DAOPKQ010000160         3251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_33182_length_3251_cov_5.913016, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000160 DAOPKQ010000000
VERSION     DAOPKQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33182_length_3251_cov_5.913016"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PK146_05880"
     CDS             complement(<1..690)
                     /locus_tag="PK146_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPE91804.1"
                     /translation="MVRSVDEVPLPVSSLFGHFFDYLSEAVVMTGQNDQILRVNRSFT
                     EMFGYTPSEVTGSNIDDVVTSEGHLKDEARQFSAANASGKSILADTMRRKKDGSLFPV
                     SVHAIPVVFGSGRKVNFIIYRDRPEGEPQSLGRDEQISAALLEHSPNPILVINPDTSI
                     RYVNRSFEKLTGYALQEIAGARIPYPWWSGDRLEINRAGLLDSFENGADKIEYLFRSR
                     SGDPFWIEATGV"
     gene            820..1737
                     /gene="glsA"
                     /locus_tag="PK146_05885"
     CDS             820..1737
                     /gene="glsA"
                     /locus_tag="PK146_05885"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HPE91805.1"
                     /translation="MERALREAVELGRDVLGQGAVATYIPELAKADPSALAAAYCGID
                     GNTISAGDKGTKITVQSISKVASLGLALKICGENTVFSRVGVEPSADPFNSIMRLEMR
                     APHRPQNPLINSGAILVLSLLPFSDSTERLEAVLDTMRRMSGNEGLRVNEAVAASERD
                     TSDRNRSLGYFLRSVEAMDGDVENILDSYFRQCAIECSVEDLAAMGATLACGGVNPLT
                     GEQVFSERTALILRALMMTCGLYDGSGDFAVRVGFPAKSGVGGGIMGSVPARAGVAVL
                     GPALDKRGNSIGGVRTLEMLSESLFLRVL"
     gene            1883..>3251
                     /locus_tag="PK146_05890"
     CDS             1883..>3251
                     /locus_tag="PK146_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HPE91806.1"
                     /translation="MDGSGNLPNRDLPLSDNAMRVLEERYLLKDGDSKIIETPDEMFW
                     RVARSVATAEEDPSDDTIVKMFHDIMARLDFLPNSPTLMNAGRQGGQLAACFVLPVED
                     SMEGIFDSLKHMALIHKSGGGTGYNFSKLRPKGDKVSSTNGIASGPISFMGMFDHATE
                     VVMQGGMRRGANMGILDADHPDIFDFIRAKTEEGKLHNFNISVGTSDNFMRAVENDDY
                     WDLLNPRSRDVVRTVRARELFRLICEMAWKTGDPGMVFLDKLNKDNALAHLGAITSTN
                     PCVSGDSLIHTVEGPKPARDLCGRKVDLLLNGKRVSSSEAGFFKTGEKPVFRLETREG
                     FSIRLTEDHPILKVSRKTRYREDTEWVAAGQLKPGDRIKINDHRSWTDWEGFGTLEEG
                     YVMGLLVGDGTIKKDKTVLSLWVPGAKAAGEENMGQGSLAVMDEALKAVQTLPHRSDF
                     QGWIPV"
BASE COUNT          743 a          865 c          978 g          665 t
ORIGIN      
        1 tacaccggtg gcctcgatcc agaaggggtc tcccgatcgg ctacggaaga gatactcgat
       61 cttgtcggcg ccgttctcga aggagtcgag caggccggcg cggtttatct caagacggtc
      121 cccggaccac cacgggtagg gtatgcgggc accggcgatc tcctgaaggg catagcccgt
      181 gagtttttcg aaggaccggt tgacgtacct tatggaggtg tcggggttga tcaccagaat
      241 ggggttgggt gagtgctcga gaagggcggc gcttatctgc tcatccctcc cgaggctctg
      301 gggctcccct tcaggccggt cacggtagat tatgaagttc accttccgtc cgctcccgaa
      361 taccacggga atagcgtgga cggagacagg gaagagggac ccgtcctttt tcctgcgcat
      421 ggtatcggcc aggatgctct tgccagaggc gttggccgct gagaactgcc tggcctcatc
      481 tttgagatga ccttcggatg tgaccacgtc gtcgatattg ctgcctgtta cctccgaagg
      541 agtgtaaccg aacatctccg tgaaggatct gttcactctc aggatctgat cgttctggcc
      601 tgtcattacg acagcctctg agagatagtc aaagaaatgg ccgaagagag aacttacagg
      661 aagaggcact tcatcgacac ttctgaccat accccgctcc cctccgttca accagatgtt
      721 tcattacgaa aggaacccag agattattac caaaaaaatg gtcctggagc aagtataatt
      781 ttcattgctt ccgacggtaa agcaagggga gggagatata tggagagagc cctgagggaa
      841 gctgtcgaac tggggagaga cgtacttggg caaggcgcgg tggcgaccta catcccggaa
      901 ctggccaagg cggatccgtc ggctcttgcg gcagcatact gcggcatcga cggcaatacc
      961 atctccgccg gggacaaggg gacaaagata accgttcagt ccatctccaa ggtggcatcc
     1021 ctgggcctgg ccctcaagat ctgcggcgag aacaccgtct tctcccgggt cggggtcgag
     1081 cccagcgccg accccttcaa ctccatcatg cgcctcgaga tgagagcgcc ccaccgcccc
     1141 cagaaccccc tgatcaactc cggagccatc cttgtcctgt ccctgcttcc tttcagtgac
     1201 agcacggagc gcctcgaggc cgtgctggat accatgcgca ggatgtcagg gaatgaaggg
     1261 ctcagagtga acgaggcggt ggcagcctct gagagggata ccagcgaccg caacaggtcc
     1321 ctgggctact tcctcaggag tgtggaggca atggatgggg acgtggagaa cattctcgac
     1381 tcctacttcc ggcagtgtgc cattgagtgc tccgttgaag acctggcagc catgggagcg
     1441 accctcgcat gcggaggggt taacccactg acaggggagc aggtcttctc agagaggaca
     1501 gccctgatcc tcagggccct catgatgacc tgcggcctct acgacgggtc cggtgacttc
     1561 gcagtcaggg tggggttccc ggccaagagc ggagtgggtg gagggatcat gggttcagtc
     1621 ccggcgaggg ccggagtggc cgttcttggt ccggccctgg ataagagagg aaactcgatc
     1681 ggaggggtca ggacccttga gatgctttca gagtcccttt tcctgagagt tctctgagcc
     1741 agaggacctg tctttcgata tcggcaagga aggtattata accgccctgg ttttgtgata
     1801 cgctagatat ggagggccat accttcataa accacatatc aagtacctct gtggaaacga
     1861 atatgaaaag gagaccaaga ctatggacgg atccggaaac cttcctaaca gggacctgcc
     1921 tttatccgac aatgccatga gggtgctcga ggagaggtat ctcctcaagg atggcgacag
     1981 caagatcatc gagacccccg acgagatgtt ctggagggtg gcaaggtccg ttgccactgc
     2041 cgaagaggat ccttcggacg ataccatcgt gaagatgttc cacgatatca tggcccgact
     2101 ggactttctc cccaacagtc ccaccctgat gaacgccgga cgccaggggg gacagctcgc
     2161 agcctgcttt gtcctgcccg tagaggacag catggagggg atctttgact ccctgaagca
     2221 catggctctt atccacaagt caggtggggg gacggggtac aacttcagca agctccggcc
     2281 caagggcgac aaggtctcaa gcacgaacgg gatagcttcg ggtcccatct ccttcatggg
     2341 tatgttcgac cacgctacgg aagtggtcat gcaggggggt atgcgaaggg gagccaatat
     2401 gggaatcctg gacgccgacc atccggatat cttcgatttc atcagggcca agaccgagga
     2461 gggcaagctt cataacttca acatctctgt gggcacctcc gacaacttca tgagggccgt
     2521 ggagaacgac gactactggg acctgcttaa ccccagatcc cgggatgtgg tccggaccgt
     2581 cagggcgagg gaactcttcc ggctgatctg cgagatggcc tggaaaacag gagaccctgg
     2641 gatggtcttc ctcgacaagc tgaacaagga caacgccttg gcgcatctgg gcgctatcac
     2701 gtcaactaat ccctgtgttt cgggggattc cttgatccat actgtcgaag ggcctaaacc
     2761 tgccagggat ctttgcggcc gtaaagtaga ccttttgctt aacgggaagc gggtttcttc
     2821 ttctgaagcg gggtttttca aaaccgggga aaaaccggtc tttcgccttg aaaccaggga
     2881 gggcttcagc atcaggctta ccgaagacca cccgatactt aaggtatcaa ggaaaacccg
     2941 ctacagggag gatacggaat gggttgctgc cgggcaactc aaaccaggcg accggataaa
     3001 gatcaacgat cacaggtcgt ggacggattg ggaaggtttt ggaactcttg aagaaggtta
     3061 tgtcatgggg cttctggtcg gcgatgggac tatcaagaag gacaaaaccg tcctttccct
     3121 ttgggtaccc ggtgcaaagg ctgccgggga agagaatatg ggacagggtt ccctggctgt
     3181 aatggacgaa gctttaaaag cagttcaaac attgccccat cgaagtgatt tccagggatg
     3241 gatcccggta a
//
LOCUS       DAOPKQ010000161         3247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_33234_length_3247_cov_5.301065, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000161 DAOPKQ010000000
VERSION     DAOPKQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33234_length_3247_cov_5.301065"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PK146_05895"
     CDS             <1..751
                     /locus_tag="PK146_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KipI antagonist"
                     /protein_id="HPE91807.1"
                     /translation="GGARNRGARSWICIGGGIDTPPVMGSRSTYLRGGLGGHEGRRLE
                     RGDILPLGAPDIFSRRGEGFVVPRELRQNYIERPVIRVIPDPREALIAPEGVECFYTS
                     SYRIGPDSDRMGYRLQGPLIRHKESPDMISDAVPWGAIQVPGSGLPIIMMADCQTTGG
                     YPKIAGVISADLGLLSMMLPGDHLSFRRCAVTEAADASRALKGDLERVRVLLADHRSR
                     PGAPSLGIPADNGAMLLKFGGEIFDVTWRII"
     gene            791..1573
                     /locus_tag="PK146_05900"
     CDS             791..1573
                     /locus_tag="PK146_05900"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HPE91808.1"
                     /translation="MTVSVDLNSDLGEGLGPWRMGDDSRIMDHVTSINIACGFHAGDP
                     GIMVSTAELALERGIAIGAHPGYPDLVGFGRREMGCSPEEIYSFCLYQVGALSGIVRA
                     RGGRLQHVKVHGALYNRGAKDETVARAISLAAGDLGEDLVIVGPPGSCFEKVCKEAGV
                     LFAAEVFADRAYTSDGSLVPRSLKGSVIEDPREAATRAVRMATVGEVPTIDGGKLKLR
                     ADTICLHGDTPGSAGMASIIRSSLEEAGVSVLPLGKFLGKRS"
     gene            1787..>3247
                     /locus_tag="PK146_05905"
     CDS             1787..>3247
                     /locus_tag="PK146_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPE91809.1"
                     /translation="MSEKEVCRDLREGRKGYDERVARGLARQPEMKEVFTTGGGIPLE
                     RLYTPMDIEGLDYCRDLGFPGSYPYTRGVQPTMYRGRFWTMRQYAGFATAAESNARYR
                     YLLEQGQTGLSVAFDLPTQIGYDSDHPMARGEVGKVGVAIDSLADMETLFREIPLGKV
                     STSMTINAPAAVLLAMYIAVGEKQGVPASELSGTIQNDILKEYIARGTYIFPPKPSMR
                     LITDIFGFCSDNVPKWNTISISGYHIREAGSTASQEVAFTLADGVAYVQAAVKAGLDP
                     NVFGQRLSFFFNAHNDFLEEVAKFRAARRLWARLMKERMGVSEPKALMLRFHTQTAGS
                     TLTAQQPENNIVRVAIQALASVMGGTQSLHTNSFDEALALPTEKSVGIALKTQQIIAY
                     ESGATSTVDPLAGSYVIESLTSEIERQALEYIDKIEEMGGMLVAIEKGYPQQEIQDAA
                     YQYQKAVESDERVVVGVNRFQVDEDGGGRELLRVDPS"
BASE COUNT          701 a          861 c         1009 g          676 t
ORIGIN      
        1 cgggggcgcc cgaaacaggg gagccaggtc ctggatatgc ataggtggtg ggatcgacac
       61 tccacctgtc atgggtagcc ggtctaccta cctgagaggc gggctgggag gacatgaggg
      121 tcgccggctc gagagagggg atatcctccc tctcggggct ccggacatct tttccagaag
      181 gggagagggt tttgtcgtcc ctcgggaact tcgccaaaac tatatcgaga ggccggtgat
      241 aagggtcatc cctgatccac gggaagctct catcgctcca gagggggtcg agtgttttta
      301 cacttcttcc tatagaatag gtcctgattc ggacaggatg ggttaccgtc ttcaggggcc
      361 tttgattcgt cacaaggaga gtcctgacat gatctccgat gccgttccgt ggggggcgat
      421 acaggtaccc ggaagtggtc tgcccataat catgatggcc gactgtcaga ccacaggagg
      481 ataccccaag atcgccgggg taattagcgc cgaccttggt cttctctcca tgatgttgcc
      541 cggggatcat ctttccttcc gtcggtgcgc cgttaccgag gccgcagatg cttcgagggc
      601 cctgaaagga gacctggaac gggtcagggt tctcctggct gatcacaggt cccggcccgg
      661 ggctccctct cttgggatac cggccgataa tggggcgatg ctattgaagt tcggggggga
      721 aatttttgac gtcacctgga ggataatatg acagggccgc ctcgcgaggt tgcccggggg
      781 agggtgggag atgactgttt ctgtggatct caacagtgat ctcggggaag ggttaggccc
      841 ctggaggatg ggagacgact cgaggatcat ggatcacgtc acctccatca acatcgcctg
      901 cggtttccac gccggtgatc ccgggataat ggtctcaacc gcggaactgg ccctggagag
      961 gggcattgcc attggagccc accccggcta ccctgacctt gtcggtttcg ggaggaggga
     1021 gatgggttgc tcacccgaag agatctactc tttctgcctg taccaggtcg gggccctttc
     1081 gggcatcgtc agggccaggg gcgggaggct gcagcacgtc aaggttcacg gggccctgta
     1141 caacaggggg gcaaaggatg aaactgtggc aagggcgatc tccctggccg ccggggatct
     1201 tggggaagat cttgtaatcg ttggtcctcc ggggtcctgc tttgagaagg tctgcaagga
     1261 ggcaggagtt ctttttgcag cagaggtgtt tgctgaccgt gcatacacat ccgacggttc
     1321 tctggtccca aggtcactga aaggatcagt gatcgaggat ccccgtgagg ccgctacccg
     1381 ggcggtgagg atggcgacgg tgggggaagt tcccaccata gatggcggaa agctgaagct
     1441 gagggcagat acgatctgtc tccacggtga cacacccgga tcggcaggaa tggcctccat
     1501 tataagatct tccctggaag aggcaggggt tagtgtgcta cccctcggga agtttctggg
     1561 gaaacggtcc tgatgttgat cgcagaagag ccatcccgga taaattgaat agaattgtct
     1621 cgcaattcaa tgaaatggtt gcatcatttt gcctgttagg gaataatatt cccgtttgct
     1681 tgaagattca cgaacggacc gcagtttgaa gtacagttca gtcaattttg cctcagatcc
     1741 tgttgatagt gaagacggca gtttgccggg aaaggggcgt ttcagagtga gtgaaaagga
     1801 agtctgcaga gatttgcgtg aaggaaggaa agggtacgac gaaagggtcg ccagggggtt
     1861 ggcgagacag cccgagatga aagaggtctt tactaccggc ggagggatcc ccctcgagcg
     1921 tctctacacc ccgatggata tcgagggcct ggactactgc agggacctgg gtttccccgg
     1981 ttcatacccc tatacgagag gcgttcagcc caccatgtac cgcgggcgtt tctggaccat
     2041 gaggcagtac gccggttttg ccaccgccgc cgagtcgaac gcaaggtatc gctaccttct
     2101 tgaacagggg cagacggggc tgtccgtagc ctttgacctt cccacccaga tcggctatga
     2161 ctccgaccat cccatggcca ggggagaggt cggcaaggtc ggggtcgcca ttgacagtct
     2221 ggccgacatg gagaccctct tcagggagat acccctcgga aaggtctcaa catcgatgac
     2281 gatcaatgcc ccggccgcgg tccttctggc aatgtacatc gccgtcggtg aaaagcaggg
     2341 ggttcctgcg tcggagcttt ccgggaccat ccagaacgac atcctcaagg agtacattgc
     2401 aaggggtaca tatattttcc cgcccaaacc ctccatgcgt ctcatcaccg atatcttcgg
     2461 attctgcagt gacaatgtcc ccaagtggaa caccatctcc atttcgggct accatataag
     2521 agaggcaggt tctacagcct cccaggaggt tgccttcact ctcgccgatg gtgttgccta
     2581 cgttcaggca gcggtaaagg ccgggctcga cccgaatgtc ttcggccaga gactctcctt
     2641 cttcttcaac gcccacaacg atttccttga agaggtcgcc aagttccgtg ctgcaagaag
     2701 gctctgggcg agactcatga aggaacggat gggcgtcagc gagcccaagg cgctcatgct
     2761 tcgcttccac acacagactg caggaagcac tttgacagcc cagcagccgg agaacaacat
     2821 tgttcgcgta gctatccagg cccttgcctc ggtaatgggc gggacccagt ccctgcacac
     2881 caacagcttt gatgaggccc ttgcgcttcc aacagagaag agcgtgggga tagcgctcaa
     2941 gacacagcag ataatagcct acgagtccgg agcgacctcc acggtcgatc cccttgccgg
     3001 aagctatgtc attgagagcc tcaccagcga gatcgagcgt caggccctgg aatacattga
     3061 caagatcgaa gagatgggcg gaatgcttgt cgccatcgag aaaggctacc cccagcagga
     3121 gatccaggac gctgcctatc agtaccagaa agccgtcgag agcgatgagc gggtagtggt
     3181 cggggtcaac aggttccagg ttgacgagga tggaggcggc agggagctcc taagggtcga
     3241 tccctct
//
LOCUS       DAOPKQ010000162         3228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_33517_length_3228_cov_3.903876, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000162 DAOPKQ010000000
VERSION     DAOPKQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33517_length_3228_cov_3.903876"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2404
                     /locus_tag="PK146_05910"
     CDS             <1..2404
                     /locus_tag="PK146_05910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE91810.1"
                     /translation="VLVTIIVTVVLGYFLFRHESGHLERNVEQVEEIYLPFLIPALWV
                     TDHESLQSQIEGIVKFSYIDRVEVRNDEGDVFAAGADPEPSLEVISHDLVYSNRGQYR
                     KIGSISLFVDRNELFSHIFEWMGSLFLLQLLLAVALSSVMAFVYHMVIGRQLFRLAGF
                     VKSADLTDPGKVFKLEHPSRQGDELQLLVDHINEMRGSVRSHILEIQEWRDLLQYVIQ
                     HDPNAIMVLDRDMRFVFVSERLLKNRELERERVIGRTFMEVLPELPEKWIAMFRRALE
                     GVEQSSDLDYVARPDGSMDMISWWCRPWYEPDGSIGGTVLYSEVITERKQMERSLFLE
                     KELFRTTLLSVGDGIISTDDEGRILIMNRVAEELTGWDLTEAREKDFKSVFHIIDEFS
                     RERSPDPVRQVLDSGEPVEMEENCILVSRGGQETPVEDSAAPIRDKEGETTGVVVVFR
                     DCSEKRERQREIAYLSYHDQLTGLYNRRFFEEELSRLDVPRNLPLSLVMIDVNGLKLF
                     NDAFGHTAGDELLGKVAGVLREECRADDIVARIGGDEFMILLPLTDRDHLDPLMARIS
                     DAMSRERVKDLPISVSLGGATKTDVDESVEKVFDMAEDQMYRRKISEKSSYHHRSVNL
                     IVEGLYSKSPEEKGHSENVSALSEAIGREMGLGQPDLDDLKTAGVIHDIGKIGVSDRI
                     LEKSGPLDEDEWQEMKRHPEIGYSILSTVNEYSPFAEIVLSHHERWDGAGYPKGLKRG
                     EIPLFSRILAVAEAYDAMVSGRSYRKAMGREEAVAELRRCAGTQFDPEVVDVFIEKVF
                     GK"
     gene            2695..>3228
                     /locus_tag="PK146_05915"
     CDS             2695..>3228
                     /locus_tag="PK146_05915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPE91811.1"
                     /translation="MSQNNRFDHKLDLRTLLPEDFDAVKQIMEKVYPTMGGSWTLEQY
                     QAMIKRFPDGQLCVEDKGKVVAVALSLVIDSAVYGERHTYRQVVGDGTLKNHDPDGDY
                     LYGIDVFVDPDYREMRLGRRLYDARKELCETLNLKGIIIGGRIPGYARYAEEMKPGKY
                     IQMVKNREIVDPVLSFQL"
BASE COUNT          790 a          802 c          956 g          680 t
ORIGIN      
        1 tgtcctcgtg acgatcattg tcaccgttgt tctggggtat ttccttttca ggcatgaatc
       61 gggtcacctg gagcggaatg tggaacaggt tgaggagatc taccttccct tcctgatccc
      121 ggctctttgg gtgacggacc acgagtccct tcagagccag atagagggga tcgtcaagtt
      181 cagctacatc gaccgggtcg aggtcaggaa cgatgaaggc gacgtcttcg ccgcaggggc
      241 cgacccggaa ccctccctgg aagtgatatc ccacgacctg gtctattcca accgcgggca
      301 atacaggaag atcggttcga tctccctctt tgttgacagg aacgaactgt tctcccatat
      361 cttcgagtgg atggggtcgc tcttccttct tcagttgctc ctggccgtgg ccctctcgtc
      421 ggtcatggca ttcgtctatc acatggtgat cgggagacag ctcttccgcc ttgccggctt
      481 cgtcaagagc gcagatctga ccgatcctgg aaaggtcttc aagcttgaac acccatccag
      541 gcagggcgat gaactccagc tccttgtcga ccacatcaac gagatgagag gcagtgtcag
      601 gagtcatatc ctggagatcc aggagtggcg agacctgctc cagtacgtga tccaacatga
      661 tcccaatgcc attatggtac tcgacaggga catgaggttc gtcttcgtga gcgagagact
      721 gctcaagaac agagagctcg aaagggagag ggtgatagga aggaccttca tggaggtctt
      781 gcctgaactg ccggagaagt ggatcgcaat gttccgccgt gcgttggaag gagtggagca
      841 gagctccgat ctggattacg tggcccgtcc tgacggtagc atggacatga tcagctggtg
      901 gtgccgtcca tggtatgaac cggacggttc catcggaggt acagtcctct actctgaggt
      961 gatcaccgaa cgcaagcaga tggagcgctc cctcttcctt gaaaaggagc tcttcaggac
     1021 caccctgctg tccgtcggtg acggcatcat ctccaccgac gacgagggga ggatccttat
     1081 tatgaaccgt gtggccgagg agctgaccgg gtgggacctg acagaggcca gggagaagga
     1141 cttcaagtca gtcttccata ttatcgacga gttttctaga gagagatctc ccgacccggt
     1201 aaggcaggtc ctggacagcg gagaaccggt cgagatggag gagaactgta tccttgtctc
     1261 gagagggggc caggaaaccc ccgtagagga cagtgcggcc ccgatcaggg acaaggaagg
     1321 ggagaccacg ggagtggttg tcgtcttcag ggattgttcc gagaagaggg agaggcagag
     1381 agagatagcg tacctcagtt accatgacca gcttaccggc ctgtacaacc gccgcttttt
     1441 cgaggaggaa ctaagtcgtc tggacgttcc ccgaaacctt ccgctgtcgt tggtcatgat
     1501 agacgtcaac ggccttaaac tctttaacga cgctttcggt cacaccgcag gagatgaact
     1561 tctgggaaag gttgccggag tgctccggga ggagtgtcga gccgacgaca tcgttgccag
     1621 gataggaggg gacgagttca tgatcctgct gcccttgacg gaccgggacc atctggatcc
     1681 ccttatggcc aggatcagcg atgccatgtc aagagagcga gttaaggatc ttcccatttc
     1741 cgtgtccctt ggaggagcca cgaagaccga tgttgatgag agcgttgaga aggtcttcga
     1801 tatggccgag gaccagatgt atcgccgcaa gatatctgaa aagagcagct atcatcacag
     1861 gtcggtgaac ctgatagtgg aagggcttta ctccaagtct cccgaagaaa aaggacactc
     1921 cgaaaacgtc agtgcgttga gcgaggccat aggccgggag atgggactgg gacagccgga
     1981 tttagacgat ctcaaaactg ccggtgtgat ccacgatata ggcaagatcg gagtaagcga
     2041 caggatcctg gaaaaaagcg gtcccctgga tgaagatgaa tggcaagaga tgaaaaggca
     2101 ccccgagatc gggtacagta tcctcagcac agtcaatgaa tacagcccct tcgcagagat
     2161 cgtgctttcc catcatgagc gatgggatgg tgcgggctat cccaagggcc tgaagagagg
     2221 ggagatccct cttttctcga ggatcctggc agtcgcggaa gcctatgacg caatggtctc
     2281 agggagatcc tacaggaaag ccatgggcag ggaggaagct gtggccgaac tccgtcgttg
     2341 cgccggcacc cagttcgatc ccgaagtggt agatgtgttc atagaaaaag tctttggcaa
     2401 gtgatggtca agaaaaagtc gacgagtgaa ggatgcaggt gataggtctg aattacccca
     2461 ctggatctgt cagaaacaaa caagcatcac aatcctatcc ccttttcccg ctcaacagga
     2521 tctcctgtag gcagatccca tactgcaaat gagatcaaaa cagaggatct tacactgtga
     2581 cacaagagaa aggccctttg gcttcagatg aacccttgca ggtagtttcc ccattgctgt
     2641 cgagggctgt ctttgggtag gcgcataggt tcaaagtttg aaaggagttt ataaatgtcg
     2701 caaaacaacc ggttcgacca caaactggat ctgcgaactc tgctacccga agacttcgac
     2761 gcagtgaaac agatcatgga gaaagtttat cccactatgg gtggctcatg gacactggaa
     2821 caataccagg caatgatcaa gaggttcccc gacggccagc tctgcgtgga ggacaagggg
     2881 aaggttgttg ccgtggcact ttcgctggtg atcgattcgg ccgtttacgg ggagcgacat
     2941 acctaccgcc aggtagtggg ggacgggacg ctcaagaacc acgaccccga cggggattac
     3001 ctttacggca ttgatgtctt cgttgaccct gattacagag agatgcgtct gggacgaagg
     3061 ctttacgacg caaggaagga actctgcgag accctcaacc tcaagggcat aatcattggc
     3121 ggacgtatac ctggttacgc ccgttacgcg gaggagatga agcccgggaa atatatccag
     3181 atggtcaaga acagggagat cgttgatccg gtcctttcgt tccagttg
//
LOCUS       DAOPKQ010000163         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_33574_length_3224_cov_3.856422, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000163 DAOPKQ010000000
VERSION     DAOPKQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33574_length_3224_cov_3.856422"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2194)
                     /gene="lpxK"
                     /locus_tag="PK146_05920"
     CDS             complement(<1..2194)
                     /gene="lpxK"
                     /locus_tag="PK146_05920"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPE91812.1"
                     /translation="MGPLLNSYLRHIRGHGGVSPWSLLAPLGWFVGACSSARNFAYDH
                     GIAASSEPPVPVVSIGNLTHGGTNKTPFVEMLARKFSDMGLSPGIVMRGYGRRGKDSL
                     FAGDNPGSRDLLGDEALLLSDKLPGVPVAVAADRLSGVEFLAERGSGVAVADDGFQHR
                     RMGRDVDIVLVDATCPFGNGRLMPDGMLREPVTSLRRAHMVVISKADQVEGEKLVELK
                     KKIQDLTGSEPFTAFLELAGWSRFSGGSLEECSILPGLPLAAFSAIGNPSSFESFLLK
                     FGIRALFHQVFRDHHRYTATELDAVFARAELEGAGAMVCTEKDIFNLPEGWEPGLPLF
                     VPEVRASLADPVKFFGSLAELLKPRLVVASNGHGEDAIGSILAKELRRRFPGANVSGF
                     SLVGRGSEYRNRGVQVSSPPSETPSGGVVKYRLKDLLGDIRSGLFMHISRQAEAWRSL
                     RGRIRTVICVGDVYLLLHTLWGQGAKPLLVATAKSTRLHGHWGLEKRLLRSRCRRVWT
                     RDPETAADLLGYGVDAFYGGNPIMDLAGDTITGIEEDRPATARVLLLPGSRERAYEDL
                     PLLLEAGTIMAKEMDCSFTLVVASTIERERLLEKVQGWEICGAGNIRRTGGEPEVAVF
                     SGDVAHAAVASDILIGLGGTANQICAGLGLPVVSIDEKGKRVQKRLLGDAESLVPARG
                     RAMAEEALSILRDPARRQSMSRAGRERMGLPGAIGSVADYVSKGLGWGK"
     gene            complement(2175..>3224)
                     /locus_tag="PK146_05925"
     CDS             complement(2175..>3224)
                     /locus_tag="PK146_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91813.1"
                     /translation="LMKGVQTNAALTGIVEVILISALSVILWFGGRLVVSGELTPGEL
                     VAFLGYLALLVQPVRVFSRVVAQVQQGLAATERVFEILDTESEVVLPENPVFLEKVRG
                     DIEFRDVSFAYKGKSWVLNNISFRLSPGEKIAIVGPTGAGKSTIADLVSRFYDPQKGS
                     VLIDGVDLREVELKTFRRQTGVVPQDPVLLKGSIRFNISYGCEDASDCDVENAAKVAG
                     IYEFIMSLPEGFDTEVGERGVTLSGGQRQRVAIARAIVRDPSILIMDEATSSLDAAVE
                     QEIQDAMRKAMSGRTSIVIAHRLATVREADRILVIEKGRIVEEGTHERLKGSGGLYSR
                     LCMLQFGDGNGSPPQ"
BASE COUNT          642 a         1049 c          866 g          667 t
ORIGIN      
        1 ttttccccca tccaagtcct tttgatacgt aatccgcgac ggaaccaatg gctccgggaa
       61 gacccatccg ttccctgccg gcccttgaca ttgactgcct ccgggccgga tcccgaagaa
      121 tggagagggc ctcttctgcc atcgcgcgcc ccctggccgg gacaagggat tcggcgtcac
      181 caagaagcct tttctgtacc cttttgccct tctcgtcgat ggagaccacc gggaggccga
      241 gacccgcgca tatctggttt gccgttccac caaggcctat aagtatgtcc gaggcgactg
      301 cggcgtgggc tacatccccg gagaagacag ccacctccgg ctctccgccg gtccttcgaa
      361 tattcccggc cccgcagatc tcccacccct gcaccttttc caaaagtctc tccctctcta
      421 tcgtggatgc aaccacaaga gtaaaggaac aatccatctc ttttgccatg atggtaccgg
      481 cctccagaag aagaggcagg tcctcgtatg ccctctccct gcttcccgga agaagaagaa
      541 cccgggcagt ggcgggacgg tcctcttcga tccctgtaat agtatcaccg gcaaggtcca
      601 taatggggtt ccccccgtag aaggcatcca ccccgtaacc gaggaggtcc gctgccgtct
      661 ccgggtccct ggtccagacc ctcctgcacc tgctccttag cagtctcttc tccaggcccc
      721 agtgtccgtg aagtcgggtg gactttgccg ttgccaccag aaggggtttt gccccctgcc
      781 cccacagggt gtggagaagc aggtaaacgt cacccacgca tatgacagtc ctgatcctgc
      841 cgcgaaggga cctccaggct tctgcctgtc tggagatatg catgaacagg ccggagcgga
      901 tatccccgag gaggtccttg agacggtatt ttaccactcc gccgctgggt gtctcgctcg
      961 gaggcgatga gacctgcacc cccctgttcc tgtattcgct ccctcttccg acaagggaaa
     1021 acccgctcac attcgcgccg gggaatcttc tgcgcagttc cttcgcgagt atgctgccga
     1081 tggcgtcctc gccatgcccg ttggaggcta cgacgagacg gggcttaagc agttcggcca
     1141 gactcccgaa gaatttgaca ggatcggcaa gggaggccct gacttcagga acaaaaaggg
     1201 gaaggcccgg ttcccacccc tcgggcaggt tgaatatatc cttctccgta cagaccatgg
     1261 ccccggcacc ctcgagttcc gccctggcaa aaacagcgtc gagttccgtc gcggtatacc
     1321 tgtgatggtc gcgaaaaacc tggtgaaaaa gggcccttat gccgaatttc aggaggaatg
     1381 actcgaaact ggaggggttc cctatcgccg agaatgccgc cagcggcaaa cctggcaaaa
     1441 tggaacactc ttccagactg ccccctgaga acctggacca tcccgccagt tcaagaaagg
     1501 ccgtgaaagg ttcacttccc gtcagatcct ggatcttttt cttcagttcc acgagtttct
     1561 cgccctctac ctggtccgcc ttggaaatga ccaccatatg ggccctcctg agggacgtta
     1621 cgggctccct tagcatgccg tcgggcatga gccttccgtt accgaaaggg caggttgcat
     1681 cgacaagtac tatgtccaca tcgcgaccca tcctgcggtg ctggaacccg tcgtcggcca
     1741 cggcaacccc ggaacccctt tccgcaagga actcaactcc ggaaagcctg tccgcggcca
     1801 cggcgacggg aacgccgggc agtttatccg aaagaagcag ggcctcatct cccagaaggt
     1861 cacggctccc ggggttgtcg cccgcgaaga ggctatcctt gcccctgcgg ccgtaacccc
     1921 tcatcactat cccgggggac agtcccatat cggagaattt cctggcgagc atctccacga
     1981 agggggtctt gttcgtcccc ccgtgagtga ggttgcctat gcttacgacg ggtaccggcg
     2041 gctcgctgga agcagcgatt ccgtgatcgt aggcgaaatt cctggccgag gagcaggcgc
     2101 ccacaaacca tccaagggga gccagcaggc tccacgggga taccccgcca tgacccctta
     2161 tatgcctgag gtaactattg aggaggggac ccattgccgt caccgaactg gagcatgcag
     2221 agccgtgaat atagcccccc ggagcctttc agtctttcgt gggttccctc ttcgacgatc
     2281 ctgccttttt ctatgaccag gatcctgtct gcctccctga ccgtagccag cctgtgggct
     2341 ataacgatgg aggtgcgtcc cgacatggcc ttcctcatgg cgtcctgtat ctcctgttcc
     2401 accgccgcat ccagggaaga ggtcgcctcg tccatgatca ggatagaggg atccctgact
     2461 attgccctgg ctatggccac tctctgtctc tggcctccgc tgagggtcac gcccctctca
     2521 ccgacctcgg tgtcgaaccc ttccggcagg gacattatga attcgtaaat tccggcaact
     2581 ttggccgcgt tctccacgtc gcagtcagag gcgtcctcgc agccgtagga gatgttgaac
     2641 ctgatgcttc ccttgagcag gaccgggtcc tgtgggacca cccccgtctg gcgccggaag
     2701 gtcttgagtt cgacttcccg caggtccaca ccatcaatga ggacacttcc cttctggggg
     2761 tcgtaaaagc gggataccag gtcggctatc gtggattttc cagcgcccgt cggcccgact
     2821 atcgctatct tctcacccgg ggagagccgg aaactgatat tgttcaatac ccatgacttc
     2881 cccttgtagg caaatgaaac gtcgcggaac tcgatatccc ccctgacctt ttcaaggaag
     2941 accgggttct cgggcagcac tacctcgctc tccgtgtcga gtatctcgaa gaccctctcc
     3001 gttgccgcga gcccctgctg aacctgggcc accaccctgg aaaataccct gacgggctgg
     3061 acaagaaggg ccaggtatcc gaggaaagcc acaagttctc ccggggtcag ttctcccgac
     3121 acgaccaggc gacctccgaa ccagagtatc accgagagag cggatataag gatcacttcc
     3181 actattcccg tcagggcggc gttggtctgt acacccttca tgag
//
LOCUS       DAOPKQ010000164         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_33617_length_3220_cov_4.762717, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000164 DAOPKQ010000000
VERSION     DAOPKQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33617_length_3220_cov_4.762717"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PK146_05930"
     CDS             <1..184
                     /locus_tag="PK146_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPE91814.1"
                     /translation="GEFRIDYPFFELDNLLGSPHNTNLVDGIFPVAAGIAADNVRAFL
                     EGQNPRSIVDREDYLF"
     gene            221..1366
                     /locus_tag="PK146_05935"
     CDS             221..1366
                     /locus_tag="PK146_05935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE91815.1"
                     /translation="MTGHFSFELPTRIEFGPGVSSTVAEVLIELGSRRPLVVTDKGVI
                     DAGIFDRIKGFLERGGIEFSVFSSVEPNPKDRDVMNGASAAREFGADSIVAIGGGSPI
                     DCAKGIGVIITHGGKIRDFEGRNAVSKETLPMVAIPTTSGTGSEVTFSSVITDTGDRF
                     KFSIRHPRIAARVALCDPELTVSMPPMLTAATGMDALTHAIEGYTARPSEPIADACAL
                     HAIGMIVRNLPKAVGSGRDMGARSSMLMGSILAGISFSHSDVAAVHCIAEALGGLYDA
                     PHGMCNAVALAPVMRYNMSFCVERYARVAEAMGLRFTTDQEGAEKAVTRVEELAVDVG
                     LPPFSSLGVREEDRERIAEFSAMNGSNASNPRPMKKEDYLALLKMMG"
     gene            complement(1379..1999)
                     /locus_tag="PK146_05940"
     CDS             complement(1379..1999)
                     /locus_tag="PK146_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE91816.1"
                     /translation="MNERTGKILDFLLTMDRAKTVLRKGYLWDGNRRESIAEHMWHLA
                     LWAMLLKDEIGFETDLLRTLKLVLTHDLVEIYAGDTYAYDEAGVAAQRAKEEKAAERL
                     FSSLPDDLAEEFRGLWDEFEAEKTPESRFARSLDHMQGFVQNCISGGKSWKENGIVRE
                     RTFLRTDPPRRTDPLFAEILNHLYKVADEEDLWGEGEKRNDPEQRS"
     gene            2173..>3220
                     /locus_tag="PK146_05945"
     CDS             2173..>3220
                     /locus_tag="PK146_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE91817.1"
                     /translation="MKIDDMYMDIPGRRKEFPALSREHNGYPLAFFDGPGGTQVPQAV
                     IDAVVSYYCTCNSNTHGFFVTTNESDRLLECAREAVASFLGAGGGRNISFGHNMTTLN
                     YTLSRGIGKALFPGDEILITQLDHEANRGPWLSLREEGVVVREVALLPDGTLDYDDLR
                     TKINENTRLVAMGFSSNALGTVNEVGLVRELTHKVGAWLLVDAVHYAPHFPIDVNALG
                     VDFLLCSAYKFYGTHIGILYSRDGLLDRIPVYTLRTQEQHAPYCIETGTLNHAAIAGV
                     KAAVEYVSTFGTGETLRARIVSGMERIGEYEHILGREIYRGLKGIKGVEVVGPDFEKG
                     KRVPTVSFTMEGMDP"
BASE COUNT          705 a          882 c          942 g          691 t
ORIGIN      
        1 tggggaattc aggatagact accccttctt tgaactggat aacctgctcg gctcgcctca
       61 caacacgaac cttgttgatg gcatcttccc agtggcggct ggtatagctg cagacaacgt
      121 aagagccttc ctcgagggcc aaaaccccag gagcattgta gacagagagg attacctttt
      181 ttaaggaccg tgaagtagca gggaggggga agaacagcca atgacaggac atttcagttt
      241 cgagcttccc accaggatcg agttcggtcc gggagtttcg tcgaccgtcg cggaggtgct
      301 tatcgaactg gggagcaggc gccccctggt cgtgacagac aagggtgtta tcgatgcggg
      361 catattcgac aggataaagg gattccttga aagagggggg atcgagttca gcgtattcag
      421 ctctgttgag cccaacccca aggacagaga tgtcatgaac ggggcatcgg ctgcaaggga
      481 gttcggggcc gactcgatcg tcgctatcgg cggaggcagc cccatagact gtgccaaggg
      541 aataggcgtg atcatcaccc atgggggaaa gatcagagac ttcgaaggca ggaacgccgt
      601 ctcgaaggag accctcccca tggttgccat ccccaccact tccggaacgg ggagcgaggt
      661 cactttcagt tcggtcatca ctgacaccgg ggacaggttc aagttcagca taaggcaccc
      721 ccggatagct gcaagggtgg cgctctgcga ccccgagctg acagtctcga tgcccccgat
      781 gctgacagct gccaccggaa tggatgcact gacccatgcg atagagggat acacggcgag
      841 gccgtcggaa ccgatcgctg atgcctgtgc ccttcacgcc atcggaatga tagtccggaa
      901 cctcccgaag gcggtcggtt cggggaggga tatgggtgcc cgctcctcga tgctgatggg
      961 gagcatcctt gcggggatat ccttttctca ctccgatgtg gccgcagtcc actgtatagc
     1021 ggaagcactg ggtggcctgt acgatgcccc ccatgggatg tgcaacgccg tggccctggc
     1081 gccggtaatg agatacaaca tgtccttctg tgttgaacgt tatgcaagag tggccgaagc
     1141 aatgggattg aggttcacca ccgaccagga aggggcggag aaggccgtga cccgtgtgga
     1201 ggagctggcc gtcgatgtgg ggctccctcc tttcagttcc cttggtgtcc gggaggaaga
     1261 ccgcgagagg atcgcggagt tctcggcgat gaacgggagc aacgcgagca acccccggcc
     1321 aatgaaaaag gaggattacc tggcacttct caaaatgatg ggttagaaag cacttgcatc
     1381 aagaccgttg ttcagggtcg ttccttttct ccccctctcc ccaaaggtct tcctcatcgg
     1441 cgaccttgta gaggtgattg aggatctcgg cgaacagcgg gtcggtccgg cggggcgggt
     1501 ccgttctgag aaaagtcctt tccctgacga tcccgttctc cttccaggat tttccgccgg
     1561 agatgcaatt ctgaacaaaa ccctgcatat ggtccaggga ccttgcaaaa cgggactcgg
     1621 gagtcttttc ggcttcaaat tcgtcccaaa ggccccggaa ttcctcggcc aggtcgtcgg
     1681 gaagtgatga gaagaggcgc tctgccgcct tttcctcctt cgctctctgg gctgccacgc
     1741 cagcttcgtc gtaggcgtag gtgtccccgg cgtatatttc gacaaggtca tgggtcagta
     1801 cgagcttgag tgtcctcagt agatctgtct caaaaccgat ctcgtccttg aggagcatcg
     1861 cccacagagc aaggtgccac atgtgttcgg caatgctttc ccttcggttg ccgtcccaca
     1921 ggtagccctt tctgagtacg gtctttgccc tgtccatggt taaaaggaaa tcaagtattt
     1981 tgcctgttct ttcgttcatc cataacacct ccggtatcgg tcagaatcag gttcccgagg
     2041 tccacaccct tccgttctga cctgaggaga gccctgaaag gagtttgctt tcgggattga
     2101 atgcaatata ttgtatttga acaaaagatc ctaacccctt atcggggaaa aatccaagga
     2161 ggaacacttc agatgaagat cgatgacatg tacatggata ttccggggcg tagaaaggaa
     2221 ttccccgccc tctcaaggga acacaacgga taccccctgg ctttctttga cggaccgggg
     2281 gggacccagg tcccccaggc ggtgattgac gccgtcgtat cctattactg tacctgcaat
     2341 tccaataccc acggattctt cgtgaccacg aacgagtctg acagactcct cgagtgtgcc
     2401 agggaagccg tggcctcgtt cctgggtgcg ggtgggggca ggaatatctc cttcggccat
     2461 aatatgacca cccttaacta taccctgagc aggggcatag gcaaggccct tttcccaggt
     2521 gacgagatac tgatcaccca gcttgaccac gaagccaacc gcggaccgtg gctttccctg
     2581 agagaggaag gggtggtcgt cagggaggtg gcgcttctgc cggacgggac actggactac
     2641 gacgatctaa ggaccaagat caacgagaac acacgtcttg tcgccatggg cttttcttcc
     2701 aacgcccttg gtactgtcaa cgaggtcggg ctggtcaggg agttgacaca caaggtcgga
     2761 gcctggcttc tagtcgatgc ggtccattac gccccccatt tccccattga cgtcaacgcc
     2821 ctcggggtgg acttcctcct ctgttcggcc tacaagttct acggtaccca tatagggatc
     2881 ctctattcgc gggacggtct tctcgaccgt atccccgtgt atacactcag gacccaggaa
     2941 cagcatgcac cctactgtat tgaaacgggg accctcaacc atgcggccat tgcaggcgtc
     3001 aaggccgctg tcgagtatgt ttccaccttt ggcacaggcg agacccttcg tgccagaatt
     3061 gtctccggaa tggagagaat aggagagtac gagcatatcc tgggccgaga gatctaccgg
     3121 ggactcaagg gcatcaaggg ggtcgaggtg gtcggtcctg actttgaaaa ggggaagagg
     3181 gtgcccacgg tatccttcac gatggagggg atggacccca
//
LOCUS       DAOPKQ010000165         3219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_33628_length_3219_cov_5.259482, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000165 DAOPKQ010000000
VERSION     DAOPKQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33628_length_3219_cov_5.259482"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..241
                     /gene="rpmG"
                     /locus_tag="PK146_05950"
     CDS             92..241
                     /gene="rpmG"
                     /locus_tag="PK146_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPE91818.1"
                     /translation="MADQIGLQCTECKRRNYVTTVNKKKQQKKLEKKKYCKWCKAHTL
                     HKETK"
     gene            263..338
                     /locus_tag="PK146_05955"
     tRNA            263..338
                     /locus_tag="PK146_05955"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Trp,seq:cca)
     gene            370..549
                     /gene="secE"
                     /locus_tag="PK146_05960"
     CDS             370..549
                     /gene="secE"
                     /locus_tag="PK146_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPE91819.1"
                     /translation="MKLMSFIREAKAELKRVTWPSRQQVWYSTLVVIAVTFLVAAYLG
                     IIDVLLTAVFSRVIR"
     gene            634..1176
                     /gene="nusG"
                     /locus_tag="PK146_05965"
     CDS             634..1176
                     /gene="nusG"
                     /locus_tag="PK146_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPE91820.1"
                     /translation="MIDKRERHWYIIQTYSGYENRVKANLQQRIATMGMDEKIFHVLV
                     PIEERVTVKDGKSKRTKRKVFPSYVLVDMILDDQSWYVVRHTPGVTGFVGAGSYPIPL
                     TEREVSEIMNKIGKEQIKPKIEIDLKPGDVVRVKAGPFEGQVGPVAEVLEDKGKVKFS
                     VTVFGRDTLVEADYTDLDKI"
     gene            1235..1657
                     /gene="rplK"
                     /locus_tag="PK146_05970"
     CDS             1235..1657
                     /gene="rplK"
                     /locus_tag="PK146_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPE91821.1"
                     /translation="MAKKVIAQIKLQLPAGKATPAPPVGPALGQHGVNIMEFCKQFNA
                     KTADQPGMIIPAVLTVFADRSFTFILKTPPASVLLKKAAGLEKGSGEPNKVKVGKVTL
                     DQVREIAELKKVDLNANDVEAAMEMIKGTAVSMGIEVV"
     gene            1759..2469
                     /gene="rplA"
                     /locus_tag="PK146_05975"
     CDS             1759..2469
                     /gene="rplA"
                     /locus_tag="PK146_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPE91822.1"
                     /translation="MKRRSKRYLAIAEKVTKEPLGSLREAITLAKENATAKFDESLEM
                     HVRLGVDPRHADQQVRSTTVLPHGTGVVKRVLVIAAGEKVKEAQDAGADFVGGEDIVP
                     KIEGGWLDFDSVIATPDMMKSVGKLGKILGPRGLMPSAKTGTVTFDVAHAVSEIKAGK
                     VEFRVDKFGIIHNSFGKAGFSFEDLYDNGKALLGAIVKAKPPATKGQYIKSLAITSTM
                     GVSFKIDPNAAVKELTAE"
     misc_feature    2502..2652
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2663..3196
                     /gene="rplJ"
                     /locus_tag="PK146_05980"
     CDS             2663..3196
                     /gene="rplJ"
                     /locus_tag="PK146_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPE91823.1"
                     /translation="MPSQSRIQQVELLREKLQEAEAVFVCEYRGLNVSRITALRAAIR
                     GAGGEMNVAKNTLMEIALHQESMPVPESVMTGPNAFTIAKGDAAAVAKVIRDFSREKG
                     NEALVIKGAILGDHILSADQVRALADLPSREVLLAQLAGTMIGPVRGLVTVLSGTTRG
                     LVTCLTRIKEQKEEKAA"
BASE COUNT          821 a          850 c          973 g          575 t
ORIGIN      
        1 cgcgagggcg gcaggaccgt cggcgccggc gtcgtcacag agatactcga taactgatcc
       61 aactttattg tgagaagagg ttcgacaatt catggcagac cagatcgggc tccagtgtac
      121 cgagtgcaag cgccgtaact acgtgaccac cgtcaacaag aagaaacagc agaagaagct
      181 ggagaagaag aagtattgca agtggtgcaa ggctcatacc ttgcacaagg aaacgaagta
      241 gtgtagaata cctttcgcgc gcaggtccgt ggctcaattg gcagagcacc ggtctccaaa
      301 accgggggtt gcaggttcaa gtcctgccgg gcctgccatt atgttcaagt tcaggaggcc
      361 gaggctttta tgaaactaat gagttttatc cgcgaggcga aagcagagct caaaagggtc
      421 acctggccca gcagacagca ggtctggtat tcgacccttg tcgtcattgc ggtgacattc
      481 ctggtcgcgg cctacctcgg gataatcgac gtactgctca cggcggtatt ctccagggtg
      541 atccggtagg aaagacccct ggtcagttgt acagatgaaa cagagccact ttctcgggag
      601 gatagggggc cgggtaaggc cccctttcaa gtgatgattg acaagagaga aagacactgg
      661 tacataattc agacctattc agggtatgag aacagggtaa aggccaacct ccagcagcgc
      721 attgccacaa tgggcatgga tgagaagatc ttccatgtcc ttgttcccat cgaagagagg
      781 gtcaccgtaa aggatgggaa gagcaagcgt accaagagga aggtcttccc cagctatgtc
      841 ctggtggaca tgatactcga cgaccagtcc tggtatgtcg tacgtcatac gcccggtgtc
      901 acgggcttcg tcggcgccgg gagctacccc ataccgctca ccgagcggga agtcagcgag
      961 atcatgaaca agatcggcaa ggaacagatc aaacccaaga tcgagataga cctcaaacct
     1021 ggagacgtgg tacgggtaaa ggcaggaccc ttcgagggcc aggtgggccc agtcgcagag
     1081 gtgctggaag acaagggaaa ggtcaagttc tccgttaccg ttttcggccg ggacaccctt
     1141 gtggaggccg actacaccga tctcgacaag atctgaagca acgtcttttt tatcgcccgg
     1201 aagaagccgg gctatgtagg agggaaattg gcaaatggca aagaaagtaa tagcacagat
     1261 caaactgcag ctgcctgcag gtaaggcgac acctgcccca cctgtagggc cggctctcgg
     1321 tcagcacggc gtgaacatca tggagttctg caagcagttc aacgccaaga cggccgatca
     1381 gcctgggatg atcatcccgg cggtcctgac ggtctttgcg gacaggagct tcacctttat
     1441 cctcaagaca cccccggcaa gcgtcctcct caagaaggct gcggggctcg aaaagggttc
     1501 cggtgagccc aacaaggtaa aggtcggcaa ggtgacactg gaccaggtcc gcgagatcgc
     1561 cgaactcaag aaggtcgatc tcaacgccaa cgacgtagag gccgccatgg agatgatcaa
     1621 gggcaccgct gtttccatgg gcatcgaggt agtctagtcc gggaagaggt aacaagggat
     1681 ccaccgccgg caaggcccgg cgtatccggt gggagagccc gacaaaggct cgtctggacc
     1741 acagggaggt aaaaggacat gaagaggaga agcaaaaggt acctcgccat tgccgaaaag
     1801 gtgacgaaag aaccgctcgg cagcctcagg gaagccatta ccctggccaa ggagaacgct
     1861 actgccaagt tcgacgagag ccttgagatg cacgttcgcc tcggcgtcga cccacgccac
     1921 gcagaccagc aggtccgcag caccactgtt ctcccccacg ggaccggtgt cgtgaagaga
     1981 gttctcgtca tcgcagcagg ggagaaggtc aaggaagccc aggacgcggg cgccgacttc
     2041 gtcggtggag aggacatagt tccgaagatc gaaggcggct ggctggactt cgattcagtg
     2101 atcgctaccc cggacatgat gaaatccgtg ggcaaactgg gcaagatcct cggtccccgt
     2161 ggtctcatgc ccagcgccaa gaccggtacg gtgacctttg acgttgccca cgccgtcagc
     2221 gagatcaagg caggaaaggt cgagttcagg gtcgacaagt tcggtatcat ccacaactcc
     2281 tttggcaagg ccgggttctc cttcgaggac ctctacgata acggcaaggc ccttctgggc
     2341 gccatagtca aggccaagcc gccggcaacc aaggggcagt acatcaagag cctggctatc
     2401 acgtcgacca tgggagtatc cttcaagatc gatcccaacg ctgcggtaaa ggaactgacc
     2461 gcggagtaga cgtactggac taaaaagcaa cagctgctat aatttcaccg ttccaacgac
     2521 cgtagacagc gggtgcgccc gatgcgctta atgtcttgcc cgccgaggtc tggatgagcg
     2581 aagaacgcat gtattgttgg tgtgttcaga actcccgggc ccggagggtc cgggagttct
     2641 ttttatgtaa ggaggtgaat gaatgccttc gcaatcgagg atccagcagg ttgagttgct
     2701 gagggagaag ctccaggaag cagaagccgt tttcgtatgc gagtacaggg gactgaacgt
     2761 gtcaaggatc acagcactga gagcggcgat ccgcggagcc ggcggcgaga tgaacgtggc
     2821 caagaatacc ctgatggaga tagccctcca ccaggaatcc atgcccgttc ccgaatctgt
     2881 catgaccggt cccaacgcct tcaccattgc caaaggggat gccgcggcag tggcaaaggt
     2941 gataagggac ttctccaggg agaagggtaa cgaggccctt gtgatcaagg gagccatcct
     3001 gggagatcac atcctctcgg cggaccaggt cagggcactg gccgatctgc cttcgaggga
     3061 ggtcctgctg gcacagctcg caggaacgat gataggccca gtcagaggcc ttgtaacggt
     3121 gctttccggt accactcgcg ggctcgttac atgccttaca aggatcaagg aacagaagga
     3181 agaaaaggca gcctgaaaaa agagatcaaa tcccaacgg
//
LOCUS       DAOPKQ010000166         3192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_34022_length_3192_cov_4.277654, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000166 DAOPKQ010000000
VERSION     DAOPKQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34022_length_3192_cov_4.277654"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK146_05985"
     CDS             <1..589
                     /locus_tag="PK146_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HPE91824.1"
                     /translation="INSLTMLVLYGVLGGFLLGVGRLPVPWQALLLSIGVYVALPLAA
                     GYYSRKWMIGARGIQWFREQFLHYLTPVTILALLTTLVLLFSFKGEVIVANPLTILWI
                     AVPLFTQTILIFAIGYALAKKLGLSYDMAAPAAMIGASNHFEVAIATATMLFGLSSGA
                     ALATVVGVLIEVPLMLMLVRFCVRTRHWFPAPEIK"
     gene            complement(1041..2207)
                     /locus_tag="PK146_05990"
     CDS             complement(1041..2207)
                     /locus_tag="PK146_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPE91825.1"
                     /translation="MSDRKKFLVLAAVFALFYFLPVESPRVHSGAVEALAMLHEYARE
                     HVILCLVPAFFIAGAISVFVSQQAVMKYLGAEARRWVAYSVAAISGTVLAVCSCTVLP
                     LFAGIYKRGAGLGPATAFLYSGPAINVLAIILTARVLGFELGLARAVGAVVFSVVIGL
                     LMAVVFRSEETERMQGFVEIPETGPVRPLWKTSTHMAGMVVFLVFANWAAPRVPGGIW
                     ETVYKMKWIIAALAFVFTLSASRVWLDSDERSQWLGSTWGYALQVVPLLFGGVLVAGF
                     LLGRPGHEALIPGTWIETLVGGNSLGANFFASVVGAFMYFATLTEVPILQGLIGAGMG
                     KGPALALLLAGPALSLPNMLVIRSILGTKKTVAYTCLVVVLSTLAGVAFGTFFG"
     gene            complement(2296..2541)
                     /locus_tag="PK146_05995"
     CDS             complement(2296..2541)
                     /locus_tag="PK146_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPE91826.1"
                     /translation="MKIQVLGTGCPKCKKMAELAEAAAADLGLNYELVKVTGISEILD
                     FGVMATPGLAVDGKVLAAGGVPTPEQMKRLLAQAAGK"
     gene            complement(2564..2830)
                     /locus_tag="PK146_06000"
     CDS             complement(2564..2830)
                     /locus_tag="PK146_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPE91827.1"
                     /translation="MKDPRVGILKAMAHPVRLGLVETLASRRLRVGELANLFPVDRTT
                     ISKHIAILREAGILETVREGREIYYRLAMPCLSDLLGCLRRMTR"
     gene            complement(2917..>3192)
                     /locus_tag="PK146_06005"
     CDS             complement(2917..>3192)
                     /locus_tag="PK146_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HPE91828.1"
                     /translation="PADPFREDPLDINSLLVRNRASTFLMRSAGEIPGWSIEEGDILV
                     VDRSCVAREGDLAVIARDGELALREVASTRIPVWGVVRGVVRVLRRG"
BASE COUNT          691 a          951 c          894 g          656 t
ORIGIN      
        1 cattaattcc cttacgatgc ttgttctcta cggtgttctg ggcggtttcc ttcttggtgt
       61 cggcaggctc ccggttccgt ggcaggcctt gctgctgtcc ataggtgtct acgtcgctct
      121 cccgctggcg gcgggctatt actccaggaa gtggatgatc ggcgccagag gtatccagtg
      181 gttcagggag cagttcctgc attatctcac ccctgtgacc attctggccc ttctgacgac
      241 cctggtcctt ctcttcagct tcaagggaga ggtcatagtg gcaaatccgc tgacgatcct
      301 ctggatagcc gtccctttat ttacccagac gatcctgata ttcgccatag gatatgctct
      361 tgcaaaaaag ctgggcctct cctacgacat ggccgctcct gcggccatga taggggcctc
      421 gaaccatttc gaggtggcaa ttgctactgc cacgatgctc ttcggcctct ccagcggcgc
      481 agccctggct accgtggtgg gtgtgcttat agaggtaccc ttaatgctga tgctcgtgag
      541 gttctgcgta agaacgcggc actggttccc cgctcctgag ataaaatagg cagccttctc
      601 tatggaagta cagctgcagg gtagaaagaa acggttcgaa agaggtgagc attttgaagg
      661 aacggatccg tattacatct aagtactggt tgcatgaagt tgcaagacac gggaacgaat
      721 tcaacatcca tatggctgta aatatagccg ggtgatgaac tgtctacagg ggcccggtgg
      781 gcctctgggg ctatccggca gaaaatacag gaaagttcag gatattttat gtggagggtc
      841 atccagggat gaaatgccac attgaggaag aggtcttcca gtggatggaa gatgcggggc
      901 cgatgcctga ggggggctac ctcttcatgg ttgagggggt cggccgcctg aggttcagca
      961 ttgtttgaat aatggaaaga ccagtgatcg ctccgaaaag tgaagaagcc ccgtccctcc
     1021 tgttgacggt ttcccccttt tcagccgaaa aaggtgccga aggccacgcc cgcaagggtt
     1081 gaaagcacca caaccaggca ggtgtatgcc acggtctttt tcgtgcccag gatgctgcgg
     1141 atgaccagca tgttgggaag cgacagtgca gggcctgcaa gcagaagggc cagggcaggt
     1201 cccttgccca ttccggcccc gataagaccc tgcaggatgg gcacctccgt aagggttgca
     1261 aagtacatga aggctcccac caccgaggcg aaaaagtttg cccccaggga gttgccgccc
     1321 accagcgttt ctatccaggt gccgggaata agggcctcgt gccccggacg gccgagaagg
     1381 aatccggcca ccaggactcc cccgaaaagc agggggacca cctgcagtgc gtaaccccag
     1441 gtcgatccca gccactgcga gcgctcgtca ctgtcgagcc agaccctgga ggctgacagg
     1501 gtaaagacga aggcaagagc ggcaatgatc catttcattt tatagactgt ctcccagatc
     1561 ccgcccggga cccttggggc cgcccagttg gcaaagacca ggaagaccac catcccggcc
     1621 atgtgagtcg aagtcttcca cagaggtctc actggtccgg tttcggggat ctcgacgaaa
     1681 ccctgcatcc tttccgtttc ttccgaccgg aaaacaaccg ccatgagaag gccgatgacc
     1741 acgctgaaga ctaccgctcc cacggccctg gccaggccaa gttcaaatcc cagcactctg
     1801 gccgtaagta ttatcgcaag gacattaatt gccggtcccg agtaaaggaa tgccgtggct
     1861 ggacccaggc cggcgcccct tttgtagatc cctgcgaaaa ggggaagcac tgtgcaggag
     1921 cataccgcca ggacagtccc cgatatggcg gccacggagt atgcgaccca ccgtcgggcc
     1981 tcggccccaa gatacttcat cactgcctgt tgcgacacga agacgcttat ggcacctgcg
     2041 atgaagaaag ctggcacaag gcagaggatc acgtgctccc ttgcgtactc atggagcatc
     2101 gccagggcct ccacggcccc ggagtgcacc ctggggcttt caacgggcag aaagtagaac
     2161 agggcaaaga cagctgcgag gacaagaaat tttttccggt cgctcaaaaa tctcacctct
     2221 tgcaagtata tgtgtagata atgccaccta taaccgcgaa aaaatccccg cccgggaatt
     2281 caggcggggg aagggttatt tccctgccgc ctgtgcaagg aggcgtttca tctgctctgg
     2341 cgtcgggaca ccccctgcgg ccagtacctt tccatccacg gccagaccag gggtagccat
     2401 gaccccgaaa tcgaggatct cggagatccc cgtgaccttt accagctcat agttgaggcc
     2461 aagatccgct gccgcggcct cagccagttc ggccatcttc ttgcacttgg ggcaccccgt
     2521 tccaagcacc tgtatcttca tccactttac cccctctcga actctaccgg gtcatcctgc
     2581 gcaggcagcc caggagatca ctcaggcaag gcatggcgag cctgtagtat atctcccgcc
     2641 cctcgcggac ggtctccagt atcccagcct ccctgaggat ggcgatgtgt ttgcttatgg
     2701 tggtcctgtc caccgggaag aggttcgcca gttcgcccac cctgagcctc cgggaggcca
     2761 atgtctccac cagacccagc ctgacggggt gggccatcgc cttgagtatg cctaccctgg
     2821 gatccttcaa gggtaacatc cccctcatgc gcctcccttg agtgtgatgg aataacactc
     2881 aaaaccgtgg caattctacc acatttatgt ctttcgtcaa ccccggcgca ggacccttac
     2941 aacccccctt accacacccc agaccgggat ccgggttgag gcgacttccc tcagtgcaag
     3001 ttcaccgtcc ctggcaatga cggccaggtc gccttcccgt gccacgcagg agcggtccac
     3061 gacaaggata tcgccttcct ctattgacca gccggggatc tccccagccg agcgcataag
     3121 aaatgtggat gcgcggttgc ggaccagcag ggagttgatg tccaggggat cctccctgaa
     3181 agggtcggcg gg
//
LOCUS       DAOPKQ010000167         3171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_34304_length_3171_cov_5.475931, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000167 DAOPKQ010000000
VERSION     DAOPKQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34304_length_3171_cov_5.475931"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PK146_06010"
     CDS             complement(<1..966)
                     /locus_tag="PK146_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91829.1"
                     /translation="MKGRPLTVQTTVALLSAFLVAVGAWPSVAATRGELINVIVSSLG
                     LPEWPGETHFADTGPDHPFRHSVETAAALGILNPSEHFYPDIEASKAEAVMFSLQAMG
                     LRHEASIVNSLTPGRWPDLPVYIAPYMTLATQIQPSPPEQFLQQPWGQISAQDLSSLR
                     SWLGECASGLSWEKEFVGENSVLVLHREHAGRPPSEWGIQSVEFGTPEEASRAAAILR
                     GMGLEAVVQALEWSWVAKVGPFQHYVKAWETMMKIPSPEMTVVPFSTDPGGALFFAAL
                     GFDPSVSPPRIVTAASISGKRLPLDLIAVNSGAEGAINGGFFSGTR"
     gene            1369..2460
                     /locus_tag="PK146_06015"
     CDS             1369..2460
                     /locus_tag="PK146_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPE91830.1"
                     /translation="MKKEDPGSSFNCAGPVRRIGITFNLKKKELLQGEPDDRYEEYDC
                     LETVETLEREITRLGFETGHYEQDETFLEKISRNPPDFVFNIAEGRGTTRGRESQVPC
                     ILESLGIPFSGSDSTAIAITLDKWLTHHVLAGGGAKVPDLYMFSTESDLGRSISIFEK
                     HSKYIVKPRWEGSSKGVFPDSVVDNPVDMAKRVRRVWKNYRQPALVEEFLPGTEITVG
                     VMGNRNPAVIGMMAIDPKAEKGNTVYSLDHKRNWEEKVRYLGPETIDPALRELVGVEA
                     LNVFRILELKDIARVDFRQDISGAPKVIDINPLPGISEAYSDLPILYRMSGGSFPSLI
                     KGILREAFQRQGLRWPRPSMKTRHCNAAR"
     gene            2447..>3171
                     /locus_tag="PK146_06020"
     CDS             2447..>3171
                     /locus_tag="PK146_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPE91831.1"
                     /translation="MRRDRVIAVVAGIEETDREDVLDVLRCRQSVIEGLVSRGFRAFP
                     VDITREDLAVKGRVARKLRENSPDCIFNLFEGFGEASYLEIRFARILESLGIPFTGNP
                     SRTLAICLDKDLARKTLQAAGLPVAPGRCVYPGYDPDIAKGLDYPLFVKPRMEDSSVG
                     IDRRSLVTSEKDLFGLLDERLALYPDGLILEEFLEGREFNAGFMGSFPYDLMAVSSIR
                     FGIGGEGKAFLGYDSKWDPESEE"
BASE COUNT          731 a          870 c          957 g          613 t
ORIGIN      
        1 cctggtcccg ctgaagaacc cgccgttgat cgctccttcg gcgccagagt taacggcgat
       61 caggtccagg gggagcctct tccctgatat ggatgctgca gtgactatcc ttggagggga
      121 gacggaggga tcgaatccca gggctgcgaa gaagagagcc cctcccggat cggtgctgaa
      181 gggcacaacg gtcatctcgg gagaggggat cttcatcatg gtctcccagg ccttcacgta
      241 gtgctgaaag ggcccgacct ttgcgaccca ggaccactcg agggcttgca ccaccgcttc
      301 cagccccatg ccccggagga tcgccgccgc ccggctggcc tcttccggtg tcccgaactc
      361 aacggactgg atcccccatt cagagggcgg tcttccagca tgttcccggt gaaggactag
      421 aacagagttc tctccgacga actctttctc ccaggaaagg ccgctggcgc actctccgag
      481 ccaggagcgc agtgaagaaa ggtcctgggc tgagatctgg ccccagggtt gctggaggaa
      541 ctgctcagga ggggagggct gtatctgggt ggccagggtc atgtagggtg caatgtaaac
      601 agggagatcg ggccatcttc caggtgtgag ggagttcaca atagaagctt cgtgacgaag
      661 gcccatagcc tggagggaga acattacggc ctctgccttg ctggcctcta tatcggggta
      721 gaagtgctcc gaggggttca gtatccccag ggccgccgca gtctcaacgg agtggcggaa
      781 ggggtggtcg ggtcctgtgt ctgcaaaatg ggtctctcca ggccactcgg ggagccccag
      841 ggaggagact atgacgttaa tcaactctcc cctggtcgct gccactgatg gccaggcccc
      901 cacagccacg agaaaggcag atagcaaggc gaccgttgtc tgtactgtca aaggtcttcc
      961 tttcatcccg gtcatccctc cccgtcactc gagggagttc tcggattgaa aaacctcccc
     1021 cttggagcct attgtaatct acaaaatgag atgtataatc cgcaaaagat agccgatcat
     1081 caagagcacg accgttggca ttgctccagg aacggcgcca gaaagcccct tgaaggcccc
     1141 cgggtgccgg ttcttaggaa ggaccctcgg cagagttgga agaaataact agacagttgc
     1201 cgaggttaac cccaagaagc cgcgagagcg ggaaagggag caaaggcagg acgtcgggct
     1261 cgaaacagcg gaggtgaagt ctgtcgaagg actggatgaa ggggcatatc accagtcacg
     1321 acaccggccg aaagggcggt gtatcaatca acgcaggggg cgaaaatagt gaagaaggaa
     1381 gatcctggat catccttcaa ttgcgcaggt ccggtccggc ggatcggcat caccttcaac
     1441 ctgaagaaga aagaacttct tcagggcgaa cccgacgacc gttacgagga atatgactgc
     1501 ctggagacgg tcgaaaccct tgagagggag atcacccggc tcggtttcga gacggggcat
     1561 tacgaacagg acgagacctt tcttgagaag atctcccgga atcctcccga ctttgttttc
     1621 aacatagccg aagggcgcgg aactacaaga gggagggaat cacaggtacc ctgcattctg
     1681 gagagccttg gcattccttt ctccggttcc gattccaccg ccattgccat cactcttgac
     1741 aagtggctga cccatcatgt gcttgctgga ggaggagcga aagtccccga tctgtacatg
     1801 ttctccacag agagcgatct ggggcggtcc atctccattt ttgaaaagca ctccaaatac
     1861 atagtcaagc ccaggtggga aggatcttcc aagggggttt tccccgattc agtggtggat
     1921 aaccctgtcg acatggcaaa gagggttcgc agggtatgga aaaattaccg gcaaccagcc
     1981 ctggtagagg aattcctccc cggtacggag atcaccgtgg gagtaatggg caacagaaac
     2041 ccggccgtca ttgggatgat ggccattgat ccgaaggcgg agaaggggaa taccgtctac
     2101 tccctggacc acaagaggaa ctgggaggag aaggtccgct acctcggccc cgagaccatc
     2161 gacccggctc tgagggaact ggtcggcgtg gaggcgctca atgtgttccg catacttgaa
     2221 ctgaaggata tcgccagggt ggatttccgt caggacatct ccggggcacc caaggtgata
     2281 gacatcaacc cgctacctgg catctccgag gcttacagcg atctgcctat cctttacagg
     2341 atgagcggcg gttcattccc ctcccttatc aaggggatcc tgagagaagc attccagaga
     2401 caggggctca ggtggcccag gccctcgatg aagacgaggc actgtaatgc ggcgcgatag
     2461 ggtgatcgcc gtcgtggccg ggatcgagga gaccgacagg gaagacgtac tggacgttct
     2521 ccgctgcaga cagagcgtca tcgaggggct cgtctccaga ggttttcggg cattcccggt
     2581 ggacatcacc agagaagacc ttgccgtgaa gggaagggtt gccagaaagt tgagagaaaa
     2641 ctcccccgac tgcatcttca acctcttcga ggggttcggc gaggcctcct acctggagat
     2701 caggtttgcc cggatactag aatccctggg tatacccttt acgggaaacc cctccaggac
     2761 tcttgccatc tgccttgaca aggatcttgc cagaaaaacc ctccaggcgg cagggttgcc
     2821 ggttgcgccc ggaaggtgcg tctacccggg ctatgacccg gatattgcga aaggccttga
     2881 ctaccctctc ttcgtcaaac ccaggatgga ggacagcagc gtaggtattg accgaagatc
     2941 cctggtgaca tcagaaaagg accttttcgg tctcctcgac gaaagacttg ccctctaccc
     3001 cgacggcctg atattggaag agttcctcga aggcagggag ttcaacgccg ggttcatggg
     3061 atccttccct tatgatctga tggccgtctc gtccatccgg tttggcatcg gaggggaagg
     3121 caaggccttc ctgggctacg attccaaatg ggaccccgag agcgaagagt a
//
LOCUS       DAOPKQ010000168         3159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_34500_length_3159_cov_5.752899, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000168 DAOPKQ010000000
VERSION     DAOPKQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34500_length_3159_cov_5.752899"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..336
                     /locus_tag="PK146_06025"
                     /pseudo
     CDS             110..336
                     /locus_tag="PK146_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            421..570
                     /locus_tag="PK146_06030"
     CDS             421..570
                     /locus_tag="PK146_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91832.1"
                     /translation="MASYRPYRPAIGLKEALEEIETKSGILYDPEVVRVCLDLFREKG
                     YQLEE"
     gene            complement(598..1494)
                     /locus_tag="PK146_06035"
     CDS             complement(598..1494)
                     /locus_tag="PK146_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPE91833.1"
                     /translation="MPRIGLALGGGGARGAAHFGVLKVLEEERIPIDIIAGTSMGAIV
                     GGMYAQHPDVPEVIDRYEKFIRNMDYESFGLKYIAPDPGQEPHLLQKFAKTIAQRIYM
                     SSMGSRSGIMKPEFLDNALKELLDRGNIQDTKIPFGAVATDLNSGRTLLIREGSIRRA
                     VRRSSLIPGYLPPEEDNGRLITDGGVSEPVPVDSAKKMGAEVVIAVSVDPSAMPRIED
                     PNMINIMRRCDLIRGVYISRIQTEKADVCICPDMSDTHWSEFLSSREFMRIGEEEARK
                     RLPEIRKATRRRRHWLFRIFSR"
     gene            1624..1911
                     /locus_tag="PK146_06040"
     CDS             1624..1911
                     /locus_tag="PK146_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91834.1"
                     /translation="MRFLMGVIGYIVGHFVLSRVHGKTRLRVGGALAVTFLVLAFFTY
                     FATYYMPPEGLEESEVLSRIAEMNARRLFLVVGEAVGISHYLFRVYRRSLI"
     gene            complement(1900..2664)
                     /locus_tag="PK146_06045"
     CDS             complement(1900..2664)
                     /locus_tag="PK146_06045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPE91835.1"
                     /translation="MLRLTRLVQILLAAVIFFSTPSAGAGHGPVYHGPRSVPVVALTF
                     DDGPHQFTTPRLIELLDRLSVKGTFFIVGSQAVQFPQILSEIVARGHSVANHSWSHRN
                     ITLLNSEDLFKELAYCSQAVEAITGMRPRFFRPPGGRWDSSSLIAAHELGLTTVLWDV
                     SGRDMVQRAPQQIAASVIRAVRPGSVVLLHGGLEWTIEALPLIVEGLRAKGFIFASLD
                     QMLPFAPEAKVPLRETSPGVARSSVVHPIKVVPSDQ"
     gene            complement(2722..>3159)
                     /locus_tag="PK146_06050"
     CDS             complement(2722..>3159)
                     /locus_tag="PK146_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPE91836.1"
                     /translation="LFAVVQLDFAITWGVLTFLLNFIPNLGSFIAAFPPILISLIQYY
                     PELGPTIIVTVGLLVIQQTIGNFLSPKVMGEKLNLSPVVILVSLLMWGWLWGVVGAIL
                     SVPITSSIKIIFDNVEPLKPLGVLMSSGKFANRARAEENNNHS"
BASE COUNT          677 a          868 c          912 g          702 t
ORIGIN      
        1 tcttgagaga acgatcctgg tcctgaccca gaccgtggaa cggagagatc cctatacagc
       61 cggacaccag aggcgtgttt cgaacctggc tgccacaata gcccgggaga tgggcctgga
      121 caccgacaag gtggaacaga tcaggatatc agggtacgtc cacgatcttg gaaagatatc
      181 agtgccggcc gagatcctga gcaaacccgg tcgactttcc gaactggaga tggacatcat
      241 cagggagcac cctgctcatg ggagggatat tctcctggat gtcgagagcg ggtgggacct
      301 ggcagagatc gtctaccagc atcacgagcg ccggacggtt cgggctaccc ccaggggctc
      361 aaaggagaag ggataagcct ggaggcaagg atacttgccg tcgcagatgt ggttgaggcc
      421 atggcctcct accgccccta caggcctgcc atagggctga aggaggctct cgaggagatc
      481 gagacaaaga gcggtatact ttacgaccct gaagtggtga gggtttgcct ggacctcttc
      541 cgggagaagg gttaccagct ggaagaatag cccgctcccg agggggatat caagggttta
      601 tcttgagaag atccggaaca accagtgccg tctcctgcgc gttgctttcc gtatctcagg
      661 aagcctcttc cttgcttctt cttcccctat cctcatgaat tccctggagg agaggaattc
      721 gctccagtgg gtatcgctca tgtcaggaca gatacacacg tcggcctttt cggtctgtat
      781 cctcgatatg tagactcccc tgataaggtc gcaccggcgc atgatgttta tcatgttggg
      841 gtcttcgatc cggggcatgg cagaggggtc caccgatacg gcaattacaa cctccgcgcc
      901 catctttttc gcagagtcaa cggggacggg ctcgctcacc cccccgtcgg ttatcagtct
      961 tccgttgtcc tcttccggag ggagatagcc gggtatcaaa gagcttcttc ggaccgctct
     1021 ccttatgctg ccctcccgga tcagaagggt tcttccagag ttcaggtcag tcgccactgc
     1081 accaaagggg atcttcgtat cctggatgtt cccacggtca agcagctcct tgagagcgtt
     1141 gtcaagaaat tcgggtttca ttatccccga ccgggacccc atggagctca tgtatatcct
     1201 ctgggctatg gtcttggcga acttctggag tagatgcggc tcctggccag ggtcaggagc
     1261 gatgtatttc aggccgaagg actcgtagtc catgttcctg atgaacttct cgtacctgtc
     1321 tatcacttcc gggacgtcgg ggtgctgcgc gtacattccc cccacgatgg cacccatgga
     1381 tgtacccgct atgatatcga tagggatcct ttcctcctcg aggaccttca gaaccccgaa
     1441 gtgggccgct ccccttgccc ctccgccccc cagggccaat cctatcctgg gcaaaacaga
     1501 accccctttg caatgttcct cttttcaggt ttatatataa cacggttgtg ccggttggca
     1561 aaaccgtacc tgagaaggga aagtgcttca ctgatgtcga ccattggaat ggagtgatcc
     1621 ggtgtgcgtt tcctgatggg ggtcataggc tacattgtcg gtcatttcgt gctgtcccgg
     1681 gttcacggga aaacccgcct tagggtcggg ggagctcttg cagtgacctt tctggtcctg
     1741 gctttcttta cctattttgc gacctattac atgccgcccg agggccttga agagtccgag
     1801 gtcctttcca ggatcgccga gatgaacgcc cggaggctct tccttgtagt cggcgaagcg
     1861 gtcggcatat cccattacct cttcagagtc tacaggagat cactgatctg aggggacgac
     1921 cttgatgggg tggaccactg aagatcgggc cactccgggg gaggtttccc ggagtgggac
     1981 ctttgcctcg ggagcaaagg ggagcatctg gtcgagagag gcaaagatga acccctttgc
     2041 ccggagaccc tccacaataa gggggagtgc ctcaatggtc cactcaagcc ccccgtgaag
     2101 gaggaccacc gaaccgggtc tgactgccct gatgacggag gccgctatct gttgtggagc
     2161 tctctgtacc atgtcccttc cgctcacgtc ccagaggacc gtggtaagtc ccagttcatg
     2221 ggcggcgatc agggagctgc tgtcccatct cccccccggt ggcctgaaga acctcgggcg
     2281 catcccggta atcgcctcaa ctgcctggct gcagtatgcc agctctttga agaggtcttc
     2341 gctgttcagg agggtgatgt tccggtggga ccaggagtgg ttcgccacgg aatgtcccct
     2401 ggccactatt tccgacagga tctggggaaa ctgtacagcc tggcttccca cgatgaagaa
     2461 ggttccttta acagagagcc tgtcgagcag ttctattagc ctgggcgtag tgaactggtg
     2521 ggggccgtcg tcgaaggtaa gggccaccac gggcacgctc ctggggccgt ggtagaccgg
     2581 tccgtggccg gcccctgcgg agggagttga gaagaaaatg actgccgcca gcaggatctg
     2641 gacgagtcgg gtcaggcgca acatctatac tgccccttat tgagcaagtt aaagggacaa
     2701 tagttcacat gaaagcaaaa gtcaagaatg attattattt tcttctgctc tcgccctgtt
     2761 ggcgaatttt cccgaggaca tgagaactcc caacggcttc aggggttcaa cattgtcgaa
     2821 gatgatcttg atagaggatg ttatcggcac cgaaaggatc gctcctacaa ccccccagag
     2881 ccaaccccac attagcagtg agaccaggat cacgaccgga ctcagattca gcttctcccc
     2941 catgaccttc ggggacagga agttgccgat ggtctgctgt attaccagga gccccacggt
     3001 gacgataatg gtcggcccca gttccgggta atactggatt agcgatatca gaatgggggg
     3061 gaatgcggcg atgaatgatc ccaggttagg tatgaaattc agaaggaagg tcagtactcc
     3121 ccatgtgata gcgaaatcca gctgcaccac agcaaaaag
//
LOCUS       DAOPKQ010000169         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_34564_length_3155_cov_4.672903, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000169 DAOPKQ010000000
VERSION     DAOPKQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34564_length_3155_cov_4.672903"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..397)
                     /locus_tag="PK146_06055"
     CDS             complement(71..397)
                     /locus_tag="PK146_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPE91837.1"
                     /translation="MGEQTLFCGVLVVQLKIPGAASLKDRRQVTRSLLERARSRLNVS
                     SADLGPEGSFQDGFLAFSAVSSSPSEAGERLDSLLRMIRSMEEKGEFLLVREIKEVER
                     YAGFQD"
     gene            complement(397..2349)
                     /gene="infB"
                     /locus_tag="PK146_06060"
     CDS             complement(397..2349)
                     /gene="infB"
                     /locus_tag="PK146_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPE91838.1"
                     /translation="MSKIRVYELAKMLGKTNREMMDLLRDLGVEVKNHMSSIESETAQ
                     LVEDAVSEGRKGPCRDSVPSRKVVVPKGALINEVAIHLGKKPGELVKILVSSGHMIPA
                     NSPVTDEILKVLSSELAIEIVTGDEQSTEESAVVSRKPLFRGDNLLERAPIITVMGHV
                     DHGKTTLLDHIRKTRITETEAGGITQHIGASTVVHKGKRIVFLDTPGHEAFTAMRARG
                     AQATDIVILVVAADDGVKPQTVEALNHAKASGVPIVVSVNKIDKPEARPDRVRQQLSD
                     YGLVPEEWGGDTIFVDISAVTGHGIEQLLEMVLIVADMLELKADPSVSPEGTVIEARL
                     DKGKGPVATVLVQQGTLKRGDIIRTDSAWGKIRAMIGDSGNQTEEAGPSTAVEILGFS
                     GVPQPGEMFTVVLSEKEARQVMNERETARKEAGSGPARKITLEEMYDRMQMGETPTLP
                     VVLKCDVQGSLEALKASLKKIETSEVGIQFVHEGVGRISESDVMLASASNAIIIGFNV
                     RPDPNAKRMAEDEGIQIRLYRIIYDVIEDVHAALEGMLAPTIREQVLGQAEIRAVFKV
                     PKAGKVAGCYVTEGLIKRGGKVRLIRDGVVYWEGNLSALRRFKDDAREVTSGYECGMS
                     FSNQQDFHEGDIVEAFELVEEKRTLD"
     gene            complement(2327..2671)
                     /locus_tag="PK146_06065"
     CDS             complement(2327..2671)
                     /locus_tag="PK146_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91839.1"
                     /translation="MNDRLSGLLGIARRAGLLFSGQDTVKAELRRGKRLLIILSGDHS
                     RNVRSMIEGYRRRGMCRVMVTGNLGREELGRLLSTSQTQIVGLPLENGLSEKIEKLLT
                     EEADRYEQDQGL"
     gene            complement(2661..2984)
                     /locus_tag="PK146_06070"
     CDS             complement(2661..2984)
                     /locus_tag="PK146_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPE91840.1"
                     /translation="MIGTATGPGKRRRPRTCAGCGGEFRRTELLRIVRSPDGEIVVDP
                     TGRAPGRGVYLCRKRSCLELARKRKQLSRSLRSQVPDEIFVSISALLDEIADRSVPEG
                     NGHER"
     gene            complement(2981..>3155)
                     /locus_tag="PK146_06075"
     CDS             complement(2981..>3155)
                     /locus_tag="PK146_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HPE91841.1"
                     /translation="FVRPDHLSLAIGKAGQNVRLAARLTKWKIDIKVLEPEKLPTLQD
                     LFEDLITPGEDGA"
BASE COUNT          598 a          989 c          775 g          793 t
ORIGIN      
        1 caatgagtcg tcctttatcc ttatcctgat caggtcggag atctcacgaa gaagctctct
       61 gttgatcctt tcaatcctga aagccggcat atctttcaac ctctttgatc tcccgaacaa
      121 gaaggaattc gcccttttcc tccatcgaac ggatcatccg caacagcgag tcgagccttt
      181 ccccggcctc ggaaggagag gacgagacag cggaaaaggc caggaaacca tcctggaatg
      241 acccctcggg accaagatcg gccgaggaga cgttgagcct gcttcgcgcc ctttccagga
      301 gtgacctggt gacctgcctt cggtccttga ggctggcggc tccgggtatt ttcagctgga
      361 ccacgaggac accgcagaag agggtctgtt cccccatcaa tcgagggtcc tcttttcctc
      421 taccagttcg aaggcttcaa caatgtcccc ttcgtggaaa tcctgctggt tcgagaagct
      481 catcccgcac tcgtaaccgg aggttacttc cctggcgtcg tccttgaacc tcctgagagc
      541 tgacaggttc ccctcccagt aaacgacgcc atccctaatc agccttacct tccctcctct
      601 tttgatcaaa ccctcggtca cgtaacaccc ggcaaccttt ccggccttgg gcaccttgaa
      661 aacagcacgt atctcggcct gccccagcac ctgctccctg atggttgggg caagcatccc
      721 ttccagggct gcgtgaacat cttcgataac gtcgtatatt atcctgtaaa gcctgatctg
      781 tatcccttca tcctcggcca tcctcttggc gttggggtcg ggcctgacgt tgaaaccgat
      841 gatgatagcg ttggatgccg aagcaagcat aacgtctgat tcggagatcc ttcctacccc
      901 ttcatggacg aactggatgc ccacctcgct ggtctctatc ttcttcaggg aggcctttag
      961 agcttccagt gatccctgga catcgcactt gagaacaaca ggcagggtgg gggtctcccc
     1021 catctgcata cggtcgtaca tctcctccag ggtgatcttt cttgccggtc ccgatcctgc
     1081 ctctttacgt gccgtctctc tctcgttcat tacctggcgg gcttccttct cgctcaagac
     1141 aacggtgaac atctcgccgg gctgtgggac accggagaaa cccagtatct caacggcggt
     1201 gctgggaccg gcctcttcag tctggttccc ggaatctcct atcatggccc tgatcttccc
     1261 ccatgccgag tcggtccgga taatgtcacc tctcttaagg gtgccctgtt gcaccagaac
     1321 ggttgccaca ggtccctttc ccttgtccag cctggcctcg atcaccgttc cttcgggaga
     1381 gaccgagggg tctgccttga gctccagcat atcggccacg ataagtacca tttcaagaag
     1441 ctgttcgatg ccatgtcctg taactgccga aatgtcaacg aaaatcgtgt ccccgcccca
     1501 ctcctccggt acaagtccgt agtcggagag ctgctgtctg accctgtcgg ggcgggcctc
     1561 gggtttatca atcttgttca cgctgaccac gatgggtact cctgaggcct tggcgtgatt
     1621 cagggcctcc actgtctgcg gcttgacccc gtcgtcagcg gcaactacaa ggatcactat
     1681 gtccgttgcc tgggcccccc tggctctcat ggcagtaaaa gcctcatgtc cgggggtatc
     1741 caggaatact atccttttcc ccttatggac caccgttgag gctccgatat gctgggttat
     1801 tcctcccgcc tcggtttcgg tgatcctggt cttccttatg tgatccagaa gggtcgtctt
     1861 cccatggtca acgtgcccca tgacggtaat tatcggagcc ctttcaagga ggttatcgcc
     1921 cctgaagaga ggttttctgc taaccactgc actctcctcg gtggactgtt catcacctgt
     1981 tactatctct atcgcgagtt ctgacgaaag gaccttcagg atctcatctg taactggcga
     2041 attggcaggg atcatgtggc ctgaagagac caggatcttg accagttccc cgggtttctt
     2101 ccccagatgg atcgccacct cattgataag agcccctttg gggacgacca cctttctgga
     2161 aggaacgctg tcgcggcaag gcccctttct cccttcgctg acggcatcct cgaccagctg
     2221 ggcagtctcg gactctattg agctcatatg gttcttgacc tctacaccga gatccctaag
     2281 gagatccatc atctccctgt tggtttttcc cagcatcttt gccagttcat agaccctgat
     2341 cttgctcata tctatcggcc tcctcggtca gcagcttttc gatcttctcc gaaagtccat
     2401 tctcaagggg cagtccgacg atctgtgtct gactggtgga aaggaggcgc cccaactctt
     2461 cccttccgag attccctgtg accatgaccc ggcacatgcc tcttctcctg tagccttcta
     2521 tcatggacct gacgttccgg gaatggtcgc ctgacagaat gatcaggagc cttttgcctc
     2581 tcctgagttc agccttgaca gtatcctgcc ccgaaaacag taaccctgcc ctccgggcga
     2641 tccccaaaag acccgaaagc ctatcgttca tggccgttcc cctccggaac ggatctgtcc
     2701 gcgatctcgt caagaagtgc ggagatggat acaaatatct catctggaac ctgcgacctg
     2761 agagatcttg aaagctgctt tctctttcgg gcaagttcca ggcagctccg ttttctgcag
     2821 agatagaccc ctcttcccgg ggctctcccg gtgggatcga cgacgatctc tccgtcgggg
     2881 ctccttacga tacgcaacag ttcggtcctg cggaactctc ctccgcaacc ggcacaggtc
     2941 ctgggtcttc ttctctttcc gggtcccgtc gccgttccga tcatgcccca tcctctccag
     3001 gggttatcag gtcctcgaaa aggtcctgga gggtgggcag cttctctggt tcgagcacct
     3061 tgatatcgat cttccacttt gtcagacgag cggccagcct gacgttctgt ccggcctttc
     3121 ctatggcaag ggagaggtga tcaggcctga cgaac
//
LOCUS       DAOPKQ010000170         3146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_34722_length_3146_cov_3.395341, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000170 DAOPKQ010000000
VERSION     DAOPKQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34722_length_3146_cov_3.395341"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PK146_06080"
     CDS             complement(<1..542)
                     /locus_tag="PK146_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPE91842.1"
                     /translation="MIRRLLRDFVLALVMVTAVSAVIYIYVKKRLAESPVHAVSRWSR
                     ARAVSTRVSQLDGTVAAALSEMELQPEDLVEWQMENPESNLRRLWHMKPADQKNASVI
                     ALYILHQNNSISPMTAWREAISFVHYSRKYEVPLTLAVAVANTESHFDPGARSPYGAA
                     GVMQVVWRVHSHLLRAHAGL"
     gene            complement(663..2414)
                     /locus_tag="PK146_06085"
     CDS             complement(663..2414)
                     /locus_tag="PK146_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE91843.1"
                     /translation="MRTLKGRMTDLIATVLVLALLSGWFLVGNTVEERMVSQAKENLL
                     SRIAILSGVIQDTGIEGLITNVSRWSRDDGTRVTLVAMNGKVLFDNEADPETMDNHAG
                     RPEIREALLSGMGINTRYSRSIEADQIYVARLAADPSGEGIVVRLSISLADVGSAVAS
                     ARRRILASLAFAGFVSLLAGLYFIRQVSRPLEELTRSALESTDGTRLRFPSTGSVEVQ
                     RLAAALEGMSSRLDDALAALEREKAYLRSLLESLPAGVLVVDREKKIRYANAALGRLL
                     RDLPEKMDGALYTGAIRKPELIDLIDRAFKGDDRRESFVVREKAEIFLEAQAVSIADG
                     VLVVVHDMTERHRLEETRRTFIADAGHEFQTPLTSIRAAAELLLDDASPDPEKTRDMA
                     GKIVLQQERMTALVDDLLLLSRLESDITPEPGEPLDLKEMIEDVVNYQKETPQAAYIA
                     WEMELPDGAPFNGRPGEIGRALGNLVDNAVKYTRKRFGETPGGVIRVSMEEQDKGWKI
                     LVGDNGTGIDEKIRETVFERFKRGETSRYREGVSPGGYGLGLAIARRIIESHGGSIEA
                     QDISEGASISVFFPFKG"
     gene            complement(2401..3111)
                     /locus_tag="PK146_06090"
     CDS             complement(2401..3111)
                     /locus_tag="PK146_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE91844.1"
                     /translation="MVERILLVEDEKTISELVAESLKRRGYLVETAFDGDTGLFAVET
                     SLPDLVILDVMLPGMDGWEVCRRIRENPATRKIPVLMLTARREERDLLAGFDVGADDY
                     MKKPFSVNELAARVGSLLRRAGAKSPAERLIRSGRLSVDLERGEAFLSGTPLDLTSTE
                     FKLLEVLAENRGRIVSRDTLLARVWGYSAADTRTVDMHVFRLRRKIEEDPENPGILLT
                     VRGRGFRLQEEGEADENP"
BASE COUNT          591 a          976 c          873 g          706 t
ORIGIN      
        1 ctgagtcccg cgtgggccct gagaaggtgt gaatggactc tccacacaac ctgcatgaca
       61 ccggcggcgc cgtaggggct ccttgcgccg gggtcgaagt ggctctcggt attggcaact
      121 gccacagcaa gggtcagggg tacctcgtac ttgcggctgt agtgtacgaa ggagatggct
      181 tcgcgccagg ccgtcatggg gctgatgctg ttgttctgat ggaggatata gagagctatg
      241 accgaggcgt ttttctggtc ggcaggcttc atgtgccaga gtcttcgaag gttcgactct
      301 gggttttcca tttgccattc aacaaggtct tcgggctgaa gttccatttc gctgagggct
      361 gcggcgacgg tgccgtcaag ctgggaaaca cgggtcgaga cggcacgggc ccggctccac
      421 ctggagacgg cgtgcaccgg ggactccgcc aggcgcttct tgacgtagat gtatatcact
      481 gccgagactg ctgttaccat gacaagcgcc agaacaaagt ccctcaggag tcttcggatc
      541 aaaagcatac ctcccttggg atattgccac cccataggac ttattccggg tggctttata
      601 ccaacccggg tattatatat acaattacag aaaaagcaac aatagtatac aataaggcgc
      661 cttcagccct taaaggggaa aaataccgat atcgaggcac cttcgctgat gtcctgggct
      721 tcgatgctgc cgccgtgtga ttcgattatc ctccgggcta tcgcaaggcc caggccgtag
      781 ccgccggggg aaacgccttc acggtagcgg cttgtctcgc cccgcttgaa acgctcgaat
      841 accgtttccc tgatcttttc atctatgccg gtgccgttgt cgcccacaag gatcttccag
      901 cccttgtcct gttcttccat ggataccctg atgaccccgc ccggggtttc accgaagcgt
      961 ttccgggtgt acttcacggc attatccaca agattcccca gggcgcggcc gatctctccg
     1021 ggtcttccat tgaagggggc cccgtcaggg agttccatct cccaggcgat gtatgcagcc
     1081 tggggcgttt ctttctggta gttaactaca tcttcgatca tctctttaag atccagcggt
     1141 tcacccggct cgggggttat gtccgactcg agccttgaca gcagaaggag gtcatccacc
     1201 agcgccgtca tccgctcctg ctgaagtact atctttccgg ccatatccct tgtcttctcc
     1261 gggtcagggg aggcatcgtc aaggagaagt tccgccgcgg cccggatcga tgtcagcggg
     1321 gtctggaatt cgtgcccggc atcggctatg aaagtcctcc tcgtctcctc gaggcggtgc
     1381 ctctcggtca tgtcgtgtac gacgaccaga actccatcgg caatggagac ggcctgggct
     1441 tcaaggaaga tctcggcctt ctcccttacg acaaatgatt cccgccggtc atccccttta
     1501 aaggccctgt cgataagatc tatgagttcg ggtttcctga tagccccggt atagagggcc
     1561 ccgtccatct tttcaggaag gtccctgagg agcctcccca gggcggcgtt ggcgtaacgg
     1621 atctttttct cccggtccac cacaaggacc ccagcgggaa gcgattcaag aagggatcgg
     1681 aggtaggcct tctcccgctc cagggcagca agggcatcgt caaggcgtga tgacataccc
     1741 tcgagggctg ccgccagccg ctgcacctcc acgctgccgg tgctgggaaa acgcagcctt
     1801 gttccgtcgg tgctctccag ggccgacctc gtcagttcct ccaggggcct ggaaacctgc
     1861 cttatgaagt agagtccggc caggagagag acaaaaccgg caaaggcaag tgaggccagt
     1921 atccttctgc gggccgatgc cacagcgctt cccacgtcgg ccagggagat agaaagcctc
     1981 accactatgc cctccccgga cggatcggct gccagccttg ccacgtagat ctggtcggcc
     2041 tctatggacc tgctgtatcg ggtatttatc cccattcccg aaaggagggc ctccctgatc
     2101 tctggtcggc ccgcatggtt gtccatggtt tccgggtcgg cctcgttatc gaaaaggacc
     2161 tttccgttca ttgccaccag ggtcaccctg gtcccgtcat cgcggctcca cctgcttacg
     2221 ttggtaatga ggccctcgat ccccgtatcc tggataaccc cggaaaggat ggctatcctc
     2281 gaaagaaggt tctccttagc ctgggagacc atcctctctt caacggtgtt ccccaccagg
     2341 aaccagcccg ataggagtgc gagcaccaga acagtggcta tcaggtcggt catgcgcccc
     2401 ttaagggttc tcatccgcct caccttcctc ctggagcctg aatccccgtc ccctgacggt
     2461 aagcaggatc cccgggtttt ccgggtcttc ctctatcttt cttctgagcc tgaagacgtg
     2521 catatccaca gtgcgggtgt cggccgccga ataaccccag accctggcca ggagtgtgtc
     2581 ccgtgatact atccttcccc tgttctccgc cagtacctcc aggagtttga actcggtgga
     2641 ggtgaggtcc agcggtgttc cgctcaggaa agcctcgccc ctctccaggt cgacggaaag
     2701 tcgcccggac ctgatgagcc tctccgcagg ggacttggcc ccggctcttc gcaggagcga
     2761 tccgaccctg gcggccagtt cgttcaccga gaagggtttc ttcatgtagt cgtcagctcc
     2821 tacgtcgaag cccgcaagga ggtcgcgctc ctcccgcctg gcggtcagca tcaaaaccgg
     2881 gatcttcctg gtggcagggt tctcgcggat acgcctgcag acctcccagc cgtccattcc
     2941 cggaagcatc acgtccagga tcaccaggtc gggaagtgat gtctcgaccg caaaaagccc
     3001 cgtgtctccg tcaaaggccg tctccaccag gtagcccctt cttttgaggc tctcagcgac
     3061 cagttccgat atggtctttt catcctcgac gagaaggatc ctctctacca tggcctattc
     3121 gttcttccct tcatggggcc tcctga
//
LOCUS       DAOPKQ010000171         3107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_35351_length_3107_cov_3.979685, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000171 DAOPKQ010000000
VERSION     DAOPKQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35351_length_3107_cov_3.979685"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PK146_06095"
     CDS             <1..971
                     /locus_tag="PK146_06095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPE91845.1"
                     /translation="ALVVFRDDAILVTDRRYLEQASRQTDLSVVDQGVTPMMEVLEKE
                     ISSRSGVWVLGFQARRVTCDAFDGLKGLGRELVDISAEFSILRRRKDPDEAKIIEEAA
                     SIASSAFIDTLSIFSNGMSERELAARLEYEMRLKGAEGGWGGQDFIVASGTRSALPHG
                     RPEDRAIKPGENVLVDFGARHMGYVSDITRTFSQGAPEKWVLEAHELLFQAQSAAIAL
                     LEPGRPASEIDAVARGVIEKAGFGDLFIHSLGHGIGLELHEAPALSPRSSDVIAVGDV
                     VTVEPGIYFPGKGGVRLEDDHYISPDGVLCLTSALKRKLFRTGARP"
     gene            complement(949..1938)
                     /gene="galE"
                     /locus_tag="PK146_06100"
     CDS             complement(949..1938)
                     /gene="galE"
                     /locus_tag="PK146_06100"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPE91846.1"
                     /translation="MLLVTGGAGYIGSHLVLGLLDRGREVLVLDNLSRGHRELTGKAV
                     FEEADIRDLSQIRRILKKYPVDGVLHFAALSLVGDSMSDPQSYYRNNIGGTMNIITSM
                     LEAGIDRIVFSSSAAVYGEPRALPVNEEAPKVPTNPYGETKLFIESMLGRSFDAYGIR
                     SVSLRYFNAAGADPLARTGEDHSPETHLVPIALDAVLGRVEELKVFGNDYPTHDGTCV
                     RDYVHVTDLADAHIRALEGLEKGAFGCEAFNLGNGKGFSVLEIVSAVRSVTGRGFPLR
                     FAPRRPGDPAVLVASSAKAEKELRWKRRFKDMDEIIETAWKWHQKRFRVGRRS"
     gene            2041..2202
                     /locus_tag="PK146_06105"
     CDS             2041..2202
                     /locus_tag="PK146_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPE91847.1"
                     /translation="MKKYLCTVCGYVYDPAVGDPDGGIEPGVAFEDLPEDWVCPVCGV
                     AKDMFEPAD"
     gene            2361..>3107
                     /locus_tag="PK146_06110"
     CDS             2361..>3107
                     /locus_tag="PK146_06110"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HPE91848.1"
                     /translation="MLDMEIILEDGSAWSGFGKGGTAQGEVVFTTASSGYPQALSDPS
                     FAGQILVFAFPMVGNYGVDEEALESSRPWVRAIVVDSLEEGRSLGTSLGEWLSLFDIP
                     FMWGVDTRSMIRHIRSKGALMGCIAPSGEGFTVMGEGKGHPVMDVSIATTEVIEGAGP
                     TIVVVDYGLKHGIQRELSARGCRVVKVPHNTSSAEILSWKPDGVLLGNGPGDPSLLKE
                     ETEVVRALLGKIPLGGICLGLQVIALASGAS"
BASE COUNT          645 a          843 c          917 g          702 t
ORIGIN      
        1 gtgctcttgt cgtattcaga gacgacgcga tactggtgac agacaggagg tacctcgagc
       61 aggcttcccg gcagacggac ctttccgtcg tggaccaggg ggtcactccc atgatggagg
      121 tccttgagaa ggagatatcg tcccgttccg gggtctgggt gctgggtttc caggctcggc
      181 gggtcacctg tgatgccttc gacggcctga agggtctcgg cagggaactg gtggatattt
      241 cggccgaatt ctcgatcctc aggagaagga aggaccccga tgaggccaag atcattgagg
      301 aagcggcctc gatcgcctcg tcagccttca ttgataccct gtcgatcttc tccaatggca
      361 tgtccgagag ggagcttgcg gcaaggctgg agtacgagat gcgcctgaaa ggagcagagg
      421 ggggttgggg tgggcaggac ttcatagttg cctcaggtac caggagcgcc ctccctcacg
      481 gcagacccga agacagggcc attaaaccgg gagagaacgt cctggtcgat ttcggagcaa
      541 ggcatatggg ctacgtcagc gatatcacca ggaccttctc acagggtgcc cctgaaaaat
      601 gggttctgga agctcacgaa cttctcttcc aggcccagtc ggcggccata gcgctccttg
      661 aacccgggag gcctgcctcg gagatcgacg cggtggcgag aggggtaatc gaaaaggcag
      721 gcttcggcga tctcttcatc cacagtcttg gacacggcat aggtcttgag ctccacgaag
      781 cgccagccct gtctccccga tcaagcgatg tgatcgccgt cggggatgtg gtgaccgtgg
      841 agcctggtat ctatttccct ggaaagggcg gtgtgaggct ggaggatgat cactacattt
      901 cacctgatgg tgtcctatgc ctcacctccg ccctgaaaag gaaactcttc aggaccggcg
      961 cccgaccctg aacctcttct ggtgccactt ccaggccgtc tcgatgatct cgtccatgtc
     1021 cttgaagcgg cgcttccacc tcagttcctt ctcggccttt gcggaggaag ccaccagtac
     1081 ggccgggtct cctggtctcc tgggagcgaa cctgagcggg aatccccttc ccgtgacaga
     1141 ccggaccgct gaaactatct ccagcaccga gaagcccttc ccgttgccaa ggttgaaggc
     1201 ttcgcacccg aaagccccct tctcgagacc ttcaagggcc ctgatatgag cgtctgcaag
     1261 gtccgtcacg tggacgtaat cccttacaca ggtcccgtca tgggtcggat agtcgtttcc
     1321 gaagaccttc agctcctcga cccttccaag cacagcatca agggcaatgg gcaccaggtg
     1381 ggtttcgggt gagtggtctt ctccggtcct ggccaggggg tccgcccctg cggcgttaaa
     1441 atacctcagg gagacactcc tgatcccgta ggcatcaaag gacctcccca gcatcgattc
     1501 tatgaagagc ttggtctcac cgtaggggtt ggtgggcact ttcggggctt cttcgtttac
     1561 cggcagggct ctgggctccc cgtagacggc tgcgctggag gagaagacta tcctgtcgat
     1621 gcccgcctcc agcatggatg taattatgtt catggtgccc ccgatgttgt tcctgtagta
     1681 ggactggggg tccgacatgg agtctcctac caggctgaga gcagcaaagt gaagcactcc
     1741 gtccacaggg tactttttca ggatccttct tatctgggaa agatctctta tgtcggcctc
     1801 ctcgaaaacg gccttgcccg tcagttccct gtgaccccgg ctgagattgt ccaggaccag
     1861 gacctcccga ccccggtcga gcaatccgag gaccaggtga cttcctatgt agcctgcacc
     1921 gccggtaact agaagcaatt ttacccctcc cgacatattg tctgcgttgt attttaccgg
     1981 aagcactcat ataatcggaa gaggcacagc caaacagatg cggcaaaagg gggaagaatg
     2041 atgaagaagt acctgtgtac tgtatgtgga tacgtgtacg accctgcagt cggtgatcct
     2101 gacggaggga tcgaaccagg tgtcgccttc gaagaccttc ccgaggattg ggtctgcccg
     2161 gtgtgcggtg ttgcaaagga catgttcgaa cccgcggact gatcaggcat atcctgttgg
     2221 tgagaacggg aggccacaag gcctcccttt tttgttattg acatatcgac gacgacgaat
     2281 ataatcattc agacatgaac caaaggtacc tcttccctga aaaataaacc attatttata
     2341 ttccccggga ggaatttcgg atgctggata tggagatcat tctggaagac ggatccgcct
     2401 ggtccggttt tggtaagggc ggtaccgccc agggagaggt ggtcttcact accgcttctt
     2461 caggttatcc acaggcattg agcgatccgt cctttgcagg tcagatactt gtctttgctt
     2521 ttcccatggt gggtaattac ggtgtcgacg aagaagctct ggagagctcc aggccctggg
     2581 tcagggcgat cgtggtcgat tcactcgagg aaggccgaag cctgggtacc tctctcggtg
     2641 aatggctttc cctgtttgat atacctttca tgtggggcgt ggatacccgt tcgatgataa
     2701 ggcatattcg ttcaaaggga gctcttatgg gctgtatagc tccttcaggg gaaggtttta
     2761 ctgtaatggg agagggaaaa ggccatcccg tcatggatgt ttccatcgca acgacggaag
     2821 tgatcgaagg agccggcccc accattgtgg tggtagatta cgggctgaag catggtattc
     2881 agagggagtt atcagcgaga ggttgccggg tggtcaaggt ccctcacaac acctcttccg
     2941 ctgagatcct ctcctggaag cctgacggtg tcctgcttgg caacggcccg ggagatccct
     3001 cccttctgaa ggaagagaca gaagtggtca gggcgctgct cggaaagatc cctctcggcg
     3061 gtatctgtct cgggctccag gtaattgcac tggcttcagg agcatca
//
LOCUS       DAOPKQ010000172         3070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_35942_length_3070_cov_4.666667, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000172 DAOPKQ010000000
VERSION     DAOPKQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35942_length_3070_cov_4.666667"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK146_06115"
     CDS             complement(<1..195)
                     /locus_tag="PK146_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HPE91849.1"
                     /translation="MVEKEDRQPLPVFVVSDFTGETAEHVAKAALSQFASDAITLHRF
                     RYINNKDRALEVLEEAETERA"
     gene            complement(236..2302)
                     /gene="glyS"
                     /locus_tag="PK146_06120"
     CDS             complement(236..2302)
                     /gene="glyS"
                     /locus_tag="PK146_06120"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPE91850.1"
                     /translation="MGKRDLLLEIGTEEIPSRFIPPALSETAAIAEEEFRAERLVFEK
                     MHTLGTPRRIVLIVRSLEERQQDKTEEFKGPAWTSGFDPSGNPTKAARGFAKSKGVDV
                     DSLEKRDIGGQEFVVAVKREEGRATSEILPKILERIIKRLSFPKSMYWDGTFNRFARP
                     VRWLLSIYGEETLKVDFGTVVSDSFTRGHRFMGVRKIEVSTVGEYLEKLHDNFVIADP
                     EKRREKMLAGISSIEKEIGGKAVLDKDLVEENLFLVEYPVPFFGAFDQSFLGIPEEVL
                     VTTMKNHQRYFPVRDSSGALKPFFIGVSNNRATNMSVVREGNERVLRARLSDASFFWE
                     EDQKIPLSARVDPLKKIVHHEKLGTVYEKVSQVRKLCGSLCEMMSLSAEEAKMVDRAA
                     LLSKADSVTDMVYEFPELRGVMGREYALKNGENPRVALALYEQYLPAFSGDRVPSDII
                     GAMVGICDRIDTITGGFKAGLQPTGSQDPYGIRRASRTLNEILWGMDLDADLLALTEV
                     SSGSRGLSEDQKEAATEFILHRLHNQLREKGYSHELTTLAISVAGSRPKQALGLLEAF
                     QEVQGADWFGGLITSAVRVRNILQKTEVSGIGLDPGLLREDAERELYDAVELLSPQVE
                     EAVGSCDWTRLTKVLSKLEPFISAFFDKVLVMDNDEHIRNNRIALLERCDGLFKTSGD
                     LGLLKT"
     gene            complement(2307..>3070)
                     /gene="glyQ"
                     /locus_tag="PK146_06125"
     CDS             complement(2307..>3070)
                     /gene="glyQ"
                     /locus_tag="PK146_06125"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPE91851.1"
                     /translation="GPEPWRVAYVEPSRRPTDGRYGQNPNRLQHYYQFQVIIQPAPEE
                     IQEIYLESLKTLGIEPADHDIRFVEDDWESPTIGAWGLGWEVWLDGMEITQFTYFQQV
                     GGIDMDPVPAEITYGIERIAMFVQKVDNVYDLEWVGKIRYGDVHHKGEVEHSTYNFEV
                     SDTEMLFRLFSMYEAEARNALSRGLVLPSYDYVLKCSHTFNLLDARNAISVTERTGYI
                     SRVRALASACCQSYAGQREEMGFPLIGKFEERTGR"
BASE COUNT          609 a          936 c          783 g          742 t
ORIGIN      
        1 tgctctttcc gtctcggcct cttcaagcac ctcgagagcc ctgtccttgt tgttgatgta
       61 acggaaccta tgcagggtta tggcatccga agcgaactgg ctcagtgccg ctttcgcaac
      121 atgttcggcc gtctcgccgg taaaatcgga gaccacaaat accggaagag gttgacgatc
      181 ttctttttca accattacca gacctccagg acccatacgg gatctgttcc aactatcatg
      241 tcttcagcaa ccccagatcc cctgatgtct tgaacagccc gtcgcacctc tcaagaaggg
      301 ctattctatt gttcctgata tgttcatcgt tgtccatcac cagcaccttg tcgaaaaagg
      361 ctgaaatgaa cggctccagc tttgaaagga ccttggtcaa ccgggtccag tcgcatgacc
      421 ctacagcctc ctcgacctgg ggtgagagaa gttccactgc atcgtagagc tctctttcgg
      481 cgtcctccct taacagaccg gggtcaagtc cgataccaga gacctcggtt ttctgaagga
      541 tgttcctgac ccttacggcc gacgtgatca ggccaccgaa ccagtcggcc ccctggacct
      601 cctggaaggc ctccagcagt ccaagtgcct gttttggtct cgatcccgcc acagagatcg
      661 caagggtggt cagctcgtgg gagtagccct tctcccgaag ctgattatgg agcctatgga
      721 gaatgaactc cgtggcggcc tctttctggt cctccgaaag tcctctcgac ccggaggaga
      781 cctccgtcag tgccagaagg tctgcatcaa ggtccatccc ccacaggatc tcgttaagcg
      841 tcctcgaagc ccttcgtatt ccgtaggggt cctgggagcc cgtcggctgg agacctgcct
      901 tgaaccctcc agtgatggtg tctattctgt cgcaaattcc caccatggca ccgatgatgt
      961 cggaggggac cctgtcaccg gaaaaggcag ggaggtactg ttcgtagaga gccagggcca
     1021 ccctggggtt ttcgccgttc ttcaaggcat actcccgccc cattactccc ctcagttcag
     1081 gaaattcgta caccatatcg gtgacgctgt ctgccttgga gagcagtgcg gcgcgatcga
     1141 ccatcttggc ctcctctgct gaaagggaca tcatctcgca aagggagccg cagagttttc
     1201 tcacctggga aaccttttcg tagaccgtcc ccagtttttc gtggtggacg atcttcttga
     1261 gaggatccac tcttgccgaa agtggtatct tctggtcctc ctcccagaag aaggaggcat
     1321 cggagagtct cgccctcagg accctctcgt taccctctct gactacggac atgttggtgg
     1381 cacggttgtt gctgacgcct atgaagaagg gtttcagggc tccggaactg tcccttaccg
     1441 ggaagtaacg ctgatggttc ttcatggtcg tgacaaggac ctcctcgggg atgccgagaa
     1501 atgactggtc aaaggccccg aagaacggca ctgggtattc gacgagaaag aggttctctt
     1561 ccaccaggtc cttgtcaagg acggcctttc ctcctatctc cttctcgatg gatgatatgc
     1621 ctgccagcat cttctccctg cgcttttcag gatctgcgat gacgaagtta tcgtgaagct
     1681 tctcaaggta ttcacccacg gtggacacct cgatctttct gacacccatg aagcgatgtc
     1741 cccttgtgaa gctgtcggag accaccgttc cgaagtcgac cttcagtgtt tcctccccgt
     1801 aaatggacag gagccacctg acaggccggg caaaacggtt gaaagtaccg tcccagtaca
     1861 tgctcttcgg gaaggagagc cttttaatta tcctttcaag tattttcggc aggatctcgg
     1921 aagttgctct cccctcctcc ctcttgacag ccacgacgaa ctcctggcct cctatgtccc
     1981 ttttctcgag ggaatcgaca tcgacaccct tgctcttcgc gaaacccctt gcggccttgg
     2041 tcggattgcc gctgggatcg aaacccgatg tccaggcagg ccccttgaac tcctcggtct
     2101 tgtcctgctg ccgttcctcc agggaccgca ctatcagaac gatcctcctg ggagttccca
     2161 gtgtatgcat cttctcgaag accagccttt cggcccggaa ctcctcttcc gcaatggccg
     2221 ccgtctcgga aagcgcaggg gggataaacc tggaaggtat ctcctctgtc cctatctcta
     2281 gaagaaggtc ccttttcccc atcgttctac ctcccggtcc tttcctcgaa tttgcctatc
     2341 agcgggaaac ccatctcttc cctctgtccg gcgtaggact gacagcaggc gcttgccagg
     2401 gcacggaccc tggagatgta ccccgtcctc tccgtaacac ttatggcgtt cctcgcatcc
     2461 agcaggttga aggtgtggga gcatttcagc acgtagtcgt aggatggcag caccaggccc
     2521 ctggaaaggg cgttcctggc ctcggcctcg tacatggaaa agagcctgaa gagcatttcc
     2581 gtatcggaga cctcgaagtt gtatgtagag tgctctactt cccccttgtg gtgaacatcc
     2641 ccatacctta ttttccccac ccattcgagg tcgtagacgt tatccacttt ctggacgaac
     2701 atggctattc tctcgatccc gtaggttatc tctgccggta ccgggtccat atcaatgccg
     2761 cccacctgct ggaaataggt gaactgggtg atctccatgc cgtcgagcca tacctcccag
     2821 ccaaggcccc aggcgcctat ggtaggcgat tcccagtcat cctcaacgaa acggatgtca
     2881 tggtcggcgg gctcgatccc caacgtcttc aagctttcca ggtagatctc ctgtatctct
     2941 tccggggccg gctgaatgat cacctggaac tggtagtagt gctgaaggcg gttgggattc
     3001 tgcccgtatc ttccgtcggt ggggcgtctt gacggctcca cgtaggccac cctccacggt
     3061 tcgggcccaa
//
LOCUS       DAOPKQ010000173         3056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_36176_length_3056_cov_4.582806, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000173 DAOPKQ010000000
VERSION     DAOPKQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36176_length_3056_cov_4.582806"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="recA"
                     /locus_tag="PK146_06130"
     CDS             complement(<1..782)
                     /gene="recA"
                     /locus_tag="PK146_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPE91852.1"
                     /translation="MGKRKGSMTREDVLDQALTDIRSKFGEGAIMCLGESSGAPAEVI
                     STGVLPLDIALGIGGYPRGRIVEIFGPEGGGKTTLALHALAEAQKAGGIAAFIDAEHA
                     LDPRLAYSLGVKIEELYVAQPDSGEQALYILETLVRSGAVDVIVVDSVAALTPQAEID
                     GKIGDSQVGLQARLMSYALRRLTSAISRSNCVVIFINQLRAKISTMGYGGPQETTTGG
                     RALKFYSSVRIEVKRGKSISKGDEALGHELWIKVVKNKQAPP"
     gene            complement(785..1381)
                     /gene="thpR"
                     /locus_tag="PK146_06135"
     CDS             complement(785..1381)
                     /gene="thpR"
                     /locus_tag="PK146_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPE91853.1"
                     /translation="MNDDLIRCFVCVELGQPVRERLSRWVARFRPMAPELRWVRDDAY
                     HITLKFCGEIPYTYLYKLENALEHGLSLKRTRAFPLELAGVGAFPGFRQPRVLWAGIG
                     GEDYQVRRVASVVERAAVAAGIEREKRPFHPHVTLARVPPTIELPVGVLREMNNSSGS
                     WGEWSVSSVTLMRSELLPEGPRYTPISILEFSNDLEVQ"
     gene            complement(1371..1946)
                     /locus_tag="PK146_06140"
     CDS             complement(1371..1946)
                     /locus_tag="PK146_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPE91854.1"
                     /translation="MVDKCRLESFLPPVESVPAEARKCQFPPGVRDLSRAVMIEARSR
                     GIRICCAESCTGGLLAGAITGNAGSSNVFTGGVVCYSNESKITILKVPGEIIERYGAV
                     SEECALAMARGAKDLFGSDIACSITGIAGPGGATPGKPVGLVWFGVVSSEESSAFPRQ
                     FQGGRGKVRRMAVVSALEALLRSMGRMGNER"
     gene            complement(1949..2407)
                     /locus_tag="PK146_06145"
     CDS             complement(1949..2407)
                     /locus_tag="PK146_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPE91855.1"
                     /translation="MWRMDKLISTCLGLGCLTGMPGTLASFAAVLAAMVLPVNAALIA
                     AFAVMGGIASWSYAREKGEDDPPEVVIDEVVGMWISLYGFGPGLFIPALGLFRILDIV
                     KPFPVRNSETLPGGVGIMADDIVAGLLANLILRGISWLFLGGGFQVLAGV"
     gene            complement(2416..>3056)
                     /locus_tag="PK146_06150"
     CDS             complement(2416..>3056)
                     /locus_tag="PK146_06150"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPE91856.1"
                     /translation="RLLYLHPGRVGRSLIEKVATSRLFLNYLDLPVQHADPDILKAMN
                     RGGLEGDKALDLFAFARTLDPGFALRTTIMVGFPGEDDRRFQRLLEFLESARIDRVGV
                     FPFYPEEGTAAAEMEDQVDEEVKRERLERVVSLQESISLQRQKLFEGEVIRVLVDEVN
                     PEEGYMEGRSYREAPEVDGVVEASAPAPVFAGEFVMVRVLEALEHDLIGEVV"
BASE COUNT          626 a         1011 c          796 g          623 t
ORIGIN      
        1 aatggcgggg cctgcttgtt cttgaccacc ttgatccaga gctcgtggcc gagggcttcg
       61 tcacccttgg agatggactt gcccctcttg acctctatcc tcacagagct gtagaacttc
      121 agtgcccttc ctccggtggt ggtctcctgg ggccccccgt aacccatggt cgatatcttt
      181 gcccttagct ggtttatgaa aataaccacg cagttgcttc tggagatcgc cgaggtgagc
      241 cttctaagcg cgtaggacat gagccttgcc tgcaggccga cctggctgtc gccgatcttt
      301 ccgtcgatct cggcctgggg ggtaagggct gcgacagaat cgacgacaat tacgtcgacg
      361 gctccgctcc tgacaagggt ctcaagaata tacagggcct gttctccgct gtcgggctgg
      421 gccacgtaga gctcctcgat cttgacccca agggaatagg ccagtcgggg gtccagggcg
      481 tgctccgcat cgatgaaggc cgctatgcct ccggcctttt gagcctccgc cagggcatga
      541 agggcgagag tcgtcttgcc tcctccttcg gggccgaaga tctcgactat cctccccctg
      601 gggtaccctc cgatacccag ggcgatatca agggggagca ctcccgtgga gatcacttcc
      661 gcaggcgctc cggagctctc tcccaggcac atgatcgcac cctcgccgaa tttgctccgg
      721 atatcggtca aagcctgatc gagcacatcc tccctggtca tggaaccttt gcgcttgccc
      781 atggtcactg cacctccagg tcatttgaga attccaggat cgaaatgggg gtatacctcg
      841 gtccttcggg cagaagttcg ctcctcataa gcgtaacgga agagacactc cactcgcccc
      901 aggatccact gctgttgttc atctccctca aaactcccac cgggagttcg atcgtcggag
      961 ggaccctggc caacgtcaca tgggggtgga atggtctttt ctccctctcg atccccgccg
     1021 ccacagccgc cctctctacg acggaggcaa ctctccggac ctgataatcc tcgcccccga
     1081 tccccgccca cagtaccctg ggctgccgga agcccggaaa ggcccccact cccgccagtt
     1141 cgagagggaa agcccttgtc ctcttgaggc tcaggccgtg ctccagggcg ttctccagct
     1201 tgtacagata agtataaggt atctccccgc agaatttcag ggtgatatgg taggcgtcat
     1261 ccctgaccca ccgaagctca ggggccatag gtctgaaccg ggccacccac cgggagagcc
     1321 tctccctgac cggttgcccg agctccacgc atacaaaaca acggataagg tcatcgttca
     1381 ttgcccatcc tccccatact tcgtaacagg gcctccaggg ccgaaacgac agccatcctc
     1441 ctgacctttc ctctgccccc ctggaactgc ctgggaaaag cgctgctctc ttctgacgag
     1501 accactccga accagaccag acccacaggt ttacccggcg tcgccccccc gggacccgca
     1561 atgcctgtta tggaacaggc tatgtcggaa ccgaaaagat cttttgcgcc ccgggccatt
     1621 gcaagggcac actcttcgct caccgctccg tatctctcga tgatctcccc ggggaccttg
     1681 aggatcgtga tcttggactc gttggagtaa cagacgacac cgcccgtgaa gacattggaa
     1741 gaaccggcgt tcccggtaat ggcgcctgca agaagcccgc cggtacatga ttctgcgcaa
     1801 cagatcctga tacccctgga ccttgcctct atcatcaccg cccgcgaaag gtccctgact
     1861 ccaggtggaa actgacattt ccttgcttcg gccgggacac tctccaccgg cggcaggaag
     1921 ctctccagcc tgcacttgtc aaccatattc aaacccctgc caggacctgg aacccgccgc
     1981 ccaggaatag ccaggagatc cccctgagga taaggttcgc cagaagaccc gctacgatat
     2041 catcagccat gatccctaca cctccgggca gtgtttccga gttcctgaca gggaagggtt
     2101 tgacgatgtc gagtatcctg aaaagcccca gggcagggat aaagagaccg ggcccaaaac
     2161 cgtagagaga tatccacatc cccaccactt catcaataac cacctcgggt ggatcatctt
     2221 cacccttctc ccttgcatat gaccaggatg ctatcccgcc catgaccgcg aacgcagcta
     2281 tcagcgccgc attgacgggg aggaccattg ctgccagcac tgcagcaaag gaggcaaggg
     2341 tcccaggcat gcccgtgagg caacccagcc caaggcaggt actgataagc ttgtccatgc
     2401 gccacatctt cactgtcaca caacctctcc gatcaggtcg tgctccagtg cctccagaac
     2461 ccggaccata acgaactctc ctgcaaagac cggcgcagga gcgctcgcct cgaccacgcc
     2521 gtcgacctcg ggagcctccc tgtatgaacg gccctccatg tacccctctt cagggttgac
     2581 ctcatcgaca agcaccctga taacttcgcc ctcgaagagt ttctgtctct ggagagagat
     2641 gctctcctgg agcgatacga cccgctccag ccgttccctc ttgacctctt cgtcaacctg
     2701 gtcttccatt tctgcggcag ctgtcccctc ttccgggtag aaggggaata ctcccaccct
     2761 gtcgatccgg gccgactcga gaaattcgag gagcctttgg aaccttctgt cgtcctcgcc
     2821 cgggaaaccc accataatag tggttctgag cgcgaatccc ggatcgagcg ttcgcgcaaa
     2881 ggcgaataga tccagggcct tatcgccctc gagtcctccc cggttcatgg ccttcaggat
     2941 atcggggtcc gcgtgctgga caggaaggtc aaggtagttg agaaagagtc tgctggtagc
     3001 cactttttcg atcagggatc tgccaaccct gccggggtga aggtaaagga gccgga
//
LOCUS       DAOPKQ010000174         3006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_37021_length_3006_cov_5.223992, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000174 DAOPKQ010000000
VERSION     DAOPKQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37021_length_3006_cov_5.223992"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PK146_06155"
     CDS             complement(<1..1266)
                     /locus_tag="PK146_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPE91857.1"
                     /translation="MSNKAILLGNEAIARGIVEAGCEVAAAYPGTPSSEILPSIAKWP
                     DDLGTRTIVEWGANEKVAFEMAAAASFAGKRSCAVMKQVGLNVAADPFMSVSHFDIKG
                     GFLLVVADDPGPHSSQTEQDSRFFAMFAKIPCFDPATAVEAKEMVFDAYDLSERHKIV
                     TMLRPGVRVDHCRQDVELKDVIDISRPASFEKDPRRWVCLPASVRVNHPQLNAKREAI
                     REEFETGFSKYNYEVPAKKKARLGVIAGGVSFSIVMDILKAWGADDISVLKIGTPHPL
                     PLRMVEDFISRHDNVVVLEETYPVIEMQLNDRTKIKGRWNGWVPGAGELLPEFIEEIL
                     CRVLGKEAPEGASAELKKAAEDLNITPRRPMLCPGCPHRASFFSIRQTLPKAIYPSDI
                     GCYTLGVNQGMVDAVVDMGASITQASGLFM"
     gene            complement(1256..1699)
                     /locus_tag="PK146_06160"
     CDS             complement(1256..1699)
                     /locus_tag="PK146_06160"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPE91858.1"
                     /translation="MGPVITTIIRVRYGETDQMGVAYYGNYLSWFEIGRTEFCRSLSS
                     PYREWEKQGVFLPAVEAYVRYKNPLRYDERVLIKTSIREVTSHSITFDYRVTPEERSR
                     LAAEGWTRHAFCGPDGRLLGEPEPFYSWLKEISRSSLEEVVQREQ"
     gene            complement(1826..2257)
                     /locus_tag="PK146_06165"
     CDS             complement(1826..2257)
                     /locus_tag="PK146_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPE91859.1"
                     /translation="MKGKVIAVCVSESKGTPKTDVGEGYLLPDVGIEGDAHAGFAHRQ
                     ISMLAVEDIEIMKERLPDLVPGSFAENITVEDFDLSSLSIGDRVRVGEALLELSQIGK
                     ECHTRCAVYHSAGDCIMPRKGLFFRVIEGGRVATGDAVERS"
     gene            complement(2254..2643)
                     /gene="nifU"
                     /locus_tag="PK146_06170"
     CDS             complement(2254..2643)
                     /gene="nifU"
                     /locus_tag="PK146_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPE91860.1"
                     /translation="MYTEKVMELFMNPKNAGRMEDPDGVGEVGNPRCGDIMKIYIKVK
                     DERIVDISFETFGCASAIASSSMVTEMVKGLTLDEALAISNKDVVNALGGLPPQKVHC
                     SLLAEEGIQAAIEDYRAKRKAGVEAKA"
     gene            complement(2649..>3006)
                     /locus_tag="PK146_06175"
     CDS             complement(2649..>3006)
                     /locus_tag="PK146_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE91861.1"
                     /translation="LIDSVTSSIEDAYLTGHRTERLPFHASFCIGSLEGESMLLRLDA
                     AGIGASSGSACTSGSLEPSYVLLAMGITHELAHGSLRFSLGKDTTREDIVYVAENLKK
                     IVADLRALSPLWKNRG"
BASE COUNT          592 a          919 c          817 g          678 t
ORIGIN      
        1 cataaagagc cccgaggcct gtgttatcga agcgcccatg tcgacaacgg catcgaccat
       61 gccctggttt acaccgaggg tatagcagcc tatgtcagag gggtagatcg ccttgggcag
      121 ggtctgcctg atcgagaaga agctggccct gtgagggcat ccggggcaga gcatcgggcg
      181 ccgcggcgtg atgttgagat cctcggcggc tttcttgagc tccgccgaag ccccctccgg
      241 agcctcttta cccagtaccc tgcagaggat ctcctcgatg aattccggaa gcagttcccc
      301 tgctccgggg acccagccgt tccatcttcc cttgatcttc gtcctgtcgt tgagctgcat
      361 ctcgatcaca gggtaggtct cctcgaggac caccacgttg tcgtgccttg atatgaaatc
      421 ctccaccatt ctgaggggaa ggggatgcgg ggtgcctatc ttaaggacgg agatatcgtc
      481 ggccccccag gccttgagaa tatccatgac tatcgagaag gagacccctc cggcaatgac
      541 cccgagcctg gcctttttct tcgcgggaac ttcgtagttg tattttgaga atccggtctc
      601 gaactcctcg cggatggcct cccgcttggc gttcagctga ggatggttca ctcttacgct
      661 ggcaggaagg cagacccagc gccttgggtc cttctcgaag gaggctggcc tggagatatc
      721 gatcacgtcc ttcagctcca catcctggcg gcagtggtcg accctgacgc cgggcctcag
      781 catcgttact atcttgtgtc tttcggagag atcataggca tcaaagacca tctcctttgc
      841 ctccacagcc gttgccggat cgaaacaggg tatcttggcg aacatggcga agaaacggct
      901 gtcctgttcg gtctgggagc tgtgagggcc tggatcgtct gcgacaacca gcaggaaacc
      961 gcccttgata tcgaagtgtg agacgctcat gaaggggtct gctgccacgt tgagacctac
     1021 ctgtttcatg accgcacatg accgcttccc tgcgaaggat gcagcggcgg ccatctcgaa
     1081 ggcgaccttt tcgttggccc cccactctac tatggttctc gtcccgagat cgtcgggcca
     1141 ctttgctatt gagggcagga tctccgacga cggtgtgccc ggataggcgg ccgccacctc
     1201 gcaaccggcc tcgacaatac cccgggcgat agcctcgttt cccaggagga tcgctttatt
     1261 gctcacgctg taccacctct tccaatgagg atctggatat ttccttcaac cagctgtaga
     1321 acggttcagg ctctccaagg agtcggccat cgggcccgca gaatgcgtgc ctggtccacc
     1381 cctccgccgc aaggcggcta cgctcctctg gcgtcaccct gtaatcgaag gtgatgctgt
     1441 gagaagtcac ctcccggatg ctcgtcttga taagaaccct ttcgtcgtat ctcagcgggt
     1501 tcttgtagcg cacataggcc tcaacagcgg gcaggaagac cccctgcttc tcccactcgc
     1561 ggtaggggga tgaaagggat ctgcagaact cggttctccc tatctcgaac catgaaaggt
     1621 agttgccgta gtaggcgacc cccatctggt ccgtctcgcc gtacctgacc cttatgatcg
     1681 ttgtgatgac aggtcccaca ggcaagacct ccctgcagat ctcgtttccc tgggaaaagt
     1741 atatgacatc ggccagaaaa aacaaaaggg cccgacgacg ggcccctgac attctgaatg
     1801 atacttcgcc tctggtggcg gttcttcagg acctctccac tgcatcgccc gtagctaccc
     1861 ttcctccttc aatgaccctg aagaagagac ccttcctggg catgatgcaa tcacctgcgc
     1921 tatggtatac ggcgcaacgg gtatgacact cctttccgat ctggctgagc tcgagcagcg
     1981 cctcgcccac cctgacacgg tcgccgatcg aaagggatga aaggtcgaaa tcctctacgg
     2041 ttatgttctc tgcgaaactg cccgggacca ggtccggcag cctttccttc attatctcta
     2101 tgtcctccac agccagcatg ctgatctgcc ggtgtgcaaa accggcatgg gcgtccccct
     2161 ctattccgac atcgggaaga aggtatccct cacccacatc ggtcttgggt gtgcccttgc
     2221 tctcggatac acagacggca attacctttc ccttcacgcc ttggcctcca ctccggcctt
     2281 ccgtttggcc cggtagtcct ctatggcggc ctggatgccc tcttcggcaa gaagcgaaca
     2341 gtggaccttc tgcggtggca agcctcccag agcgttaaca acgtccttgt ttgatatggc
     2401 gagagcctcg tccagggtga gtcccttgac catctccgtt accatcgagc tcgatgcgat
     2461 ggccgaagcg caaccgaagg tctcgaagga gatgtccacg atcctctcgt ccttcacctt
     2521 gatgtagatc ttcatgatat ccccgcaccg gggattcccg acttcgccaa ccccgtcggg
     2581 atcttccata cgccccgcgt tcttcgggtt catgaatagt tccattactt tttcagtgta
     2641 catgacattt accccctgtt tttccagagc ggggagaggg ccctgagatc ggcgacgatc
     2701 ttcttcaggt tctccgccac atagacgata tcttcccggg tagtatcctt gccgagggag
     2761 aaacggagtg aaccgtgggc cagttcatgg gttatgccca tagccagaag gacgtagctc
     2821 ggctcaaggc tgccggaggt gcaggccgac ccgctggagg cacctatccc cgccgcgtca
     2881 aggcgaagga gcattgattc tccctccagg gaaccaatgc agaaactggc atggaaggga
     2941 agtctctccg tgcggtgacc ggtcaggtat gcatcctcta tggaggaagt gacgctgtca
     3001 ataagc
//
LOCUS       DAOPKQ010000175         2995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_37224_length_2995_cov_5.810204, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000175 DAOPKQ010000000
VERSION     DAOPKQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37224_length_2995_cov_5.810204"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..980)
                     /locus_tag="PK146_06180"
     CDS             complement(21..980)
                     /locus_tag="PK146_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE91862.1"
                     /translation="MLTLKKPGIRQGIAASVLSVFLMVSFLALSAHPGLSAQGMLTVG
                     SKIDTEGSLLGQMIVILLRSKGFKVIDRTGLGATSVVRQAIIAGEIDIYPEYTGNGYY
                     FFEDSDAGVWKDPESAHRRVSALDLEKNGIVWLSPAPANNTWAIAVRKDLAQQESLST
                     LEDLAAFVNSGGRIRLACSEEFATRPDALPAFEDAYGFKLDSGSLLILSGGNTAQTEQ
                     AASLGRDGVNAAMAYGTDGGIAVLDLLVLEDTLGVQPVYEPSPIIRKEILDAYPEIAG
                     IMDPVFERLDLLTLQTLNGRIAVEGQDPARVAGEFLRSQGFVD"
     gene            1245..2528
                     /locus_tag="PK146_06185"
     CDS             1245..2528
                     /locus_tag="PK146_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HPE91863.1"
                     /translation="MSVQATAASAVFVLTIIAIATGRMRRSVATLLGASLLMAFNVIP
                     AHRAVAYIDFNTIGLLMGMMIIVGVISRTGLFQYIAVMTVKATGGRTHLTFISVLAVT
                     ALLSAFLDNVTTVLLVSPVVISLVDLMDVNPIPFLVGEAFASNIGGTATLVGDPPNII
                     IGSFANFSFMDFIFYLTPAAVLVLAVVTAWLLFHFRKDLVGGERAAERIAQVDESRLI
                     KDRHLMVRASAAMILVVTGFGIHHLLELPASVIALLGAALLLAAVPVNGDEIIHRDIE
                     WPTILFFASLFIIVGALNETGVIAATSGLFGSLFGGRPLLAAIAVLWFSGLVCAFVNN
                     VAFTATFVYVVRDMATATGMDPGPLFWALALGACLGGNGTFMGSAANVIVADIAEKSG
                     NRIHFRTFMRTGLKVVVISLTTASLYVVVRWGGLF"
     gene            2602..>2995
                     /locus_tag="PK146_06190"
     CDS             2602..>2995
                     /locus_tag="PK146_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPE91864.1"
                     /translation="MLVGDLMDRDLTAVSPETTVAEAVEIMAGHRISGVPVVDEEGKV
                     MGFLSEKDVVRAALPGYFDLLQDSSFLPDYGQFRKRLQRVSLERVDKYMKSDVIVFEE
                     QDTDLHAAMALISRNLKRAPVVRDGIFSG"
BASE COUNT          622 a          803 c          838 g          732 t
ORIGIN      
        1 cacgcatatc cgggtggcat ctagtctacg aacccctggg aacggaggaa ttctccggcc
       61 acccttgcag ggtcctgtcc ttccacggcg atccggccgt tcaaagtctg aagtgtcagg
      121 aggtccagcc tttcaaagac cgggtccata atgccggcga tctctggata tgcgtcaagg
      181 atctccttcc tgattatcgg ggaaggctcg tagacgggct gtaccccgag ggtatcctcc
      241 aggaccagaa gatcaaggac ggctatgcct ccatcagtac cgtaagccat ggcggcgttt
      301 actccgtccc gaccgagaga tgcggcctgt tccgtctggg ccgtgttccc tccggagagg
      361 atcagcaggc ttccgctgtc aagcttgaaa ccgtaggcat cctcgaaagc cggtaaagcg
      421 tcgggccgtg tggcgaactc ctcggaacag gcaagtcgga tccttccgcc actgttgacg
      481 aaggccgcaa ggtcttcaag agttgacaga ctttcctgtt gggcaaggtc ctttcttacg
      541 gcaatggccc aggtgttgtt cgccggagcc ggacttagcc atacgatgcc gttcttttcc
      601 aggtccaggg cggacaccct ccggtgggcg ctttccgggt ccttccatac cccggcgtcg
      661 ctgtcctcga aaaagtagta gccgttcccg gtgtattcag ggtatatatc tatctctccg
      721 gcaattatag cctgcctgac cacgctggtc gctcccaggc cagttctgtc aatgaccttg
      781 aaaccctttg atctaagcag tataactatc atctgtccca gcaggcttcc ctcggtatct
      841 atctttgaac ccacagtcag catgccctgg gcggaaagac cggggtgagc cgaaagggca
      901 agaaaggata ccatcaggaa aactgaaagg accgaggctg ctatcccttg tcgtatgccg
      961 ggttttttta atgtcaacat gacgtcacct ccggttcgat tttgttcatc actttacaac
     1021 aatgatagtc cgtcgctcag atcctgtcaa aattgctgtg cggactgatg aatattgata
     1081 gttcctggga cctgggcgat cttcggcagg ggaggttgac gggatctttc gatctgcagc
     1141 cttgaacccg gagcgaggga cattgataat acattgataa tacgaggaaa gccaggatct
     1201 tccaatgcct gccgggaccg gatgcaggag aaagggtctc ctttatgtca gttcaggcaa
     1261 cagcagcatc tgcagtattt gttcttacga tcatcgcaat agctaccgga aggatgagga
     1321 ggagcgtcgc aacccttttg ggcgcaagcc tgctcatggc tttcaatgtc atacctgccc
     1381 acagggctgt tgcctacatc gacttcaaca ccatcggcct gctcatgggg atgatgataa
     1441 ttgtgggcgt catctccagg acaggcctgt tccagtatat cgcagtcatg acggtcaagg
     1501 ccaccggggg caggacccat ctcaccttca tctccgtcct ggccgtcacg gcccttcttt
     1561 ctgcttttct tgacaatgtg accacggtcc ttctggtcag cccggtggtg atctcactgg
     1621 ttgacctgat ggatgtgaac cctatcccct tccttgtagg agaggccttt gcctccaata
     1681 tcggagggac ggcaactctt gttggagatc cgccgaacat aatcatcggg tcattcgcga
     1741 acttctcctt catggatttc attttctacc tgacacctgc cgccgtactg gtcctggccg
     1801 tcgtcaccgc ctggctcctc tttcactttc gcaaggatct ggtcggcggg gagagggctg
     1861 ccgagagaat agcccaggta gatgagtcaa gactgataaa ggacaggcat ctcatggtcc
     1921 gtgcctcggc cgccatgatc cttgtggtta ccggtttcgg aatacaccat ttgcttgagc
     1981 tgcctgcctc ggtcatagct ctcctgggcg cggccctgct ccttgccgca gtacccgtaa
     2041 acggcgacga gatcattcac agggatatcg agtggcctac catactgttc tttgcttccc
     2101 tcttcataat tgtcggagca ctcaacgaga cgggtgtcat agctgccaca tcgggactgt
     2161 tcggttcact cttcggcgga aggcctctgc tggcagccat agccgttctg tggttctcgg
     2221 ggcttgtctg tgccttcgtc aataacgtgg cattcacggc cacctttgta tacgtggtcc
     2281 gtgacatggc aacagcaacc ggaatggatc cggggccgct gttctgggcc ctggctctcg
     2341 gggcatgtct gggggggaat gggaccttca tgggatctgc ggcgaatgtc attgtcgccg
     2401 atatcgctga aaaaagcggt aaccggatcc atttcaggac attcatgagg acgggactga
     2461 aagtagtagt aatatcactg accaccgcaa gcctttatgt ggttgtcaga tggggaggtc
     2521 ttttctgaca ggtgtggcct tgctttacct ctttgtctgt aaagaggata cttgtaggga
     2581 ccaaagaaag gaggttccct gatgcttgtg ggagatctta tggacaggga ccttacggcc
     2641 gtatccccag agacgaccgt agcagaggcc gtggagatta tggccggtca caggatatcg
     2701 ggagttcctg tggtcgatga agagggcaag gtgatgggct tcctgagtga gaaggacgtt
     2761 gtgagggcgg ctctccccgg ctattttgac ctcttgcagg actcctcctt tctcccggat
     2821 tacggtcagt tcaggaaaag gcttcagagg gtcagtctcg aaagggtcga caagtacatg
     2881 aaaagcgatg tgatagtctt tgaagaacag gatacggacc tgcatgcagc gatggccctt
     2941 atctccagaa accttaagag ggctccagtc gtgagagatg gcatcttttc cgggg
//
LOCUS       DAOPKQ010000176         2992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_37292_length_2992_cov_4.642833, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000176 DAOPKQ010000000
VERSION     DAOPKQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37292_length_2992_cov_4.642833"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PK146_06195"
     CDS             <1..653
                     /locus_tag="PK146_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPE91865.1"
                     /translation="IRYATSHPRDLTRDVVEVMSEHENICPAINLPIQSGSDRVLGLM
                     NRGYTVGQYCSIIDMIRSILPGPAVTSDLIVGFPGETSEDFKGSVRVVEDLRFDLVHT
                     AAFSPREGTPAARMEAQVEAEEKQRRLRVINSIQSVISREINQGLVGKVFTVLADGNA
                     PKGEGLLQGRTPTDKVVIFPGDLSMMGSFVDLRITAAGNWHLFGEPVPPSWYDRRE"
     gene            663..1319
                     /locus_tag="PK146_06200"
     CDS             663..1319
                     /locus_tag="PK146_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPE91866.1"
                     /translation="MEIPGSRKGLLFLAGGVLCLVLAGFLVATFSGKWDKSPSGQVAG
                     PAPVSSARASSTSGSPGGPDAPQLQGVSDGQWVIYITGAVMRPGVYRLPAGTRVYQAV
                     EQAGGLSSEADPEAFNMALPLSDGVHVHVPRKGDPPGQPAGYQTNDHGPVQGLVDINR
                     ADLATLQHLPGIGPKIADEIIRFREERGRFSSVEDLLSVKGIGEKKLSAIKDLVTVSP
                     "
     gene            1406..2728
                     /locus_tag="PK146_06205"
     CDS             1406..2728
                     /locus_tag="PK146_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPE91867.1"
                     /translation="MSVGASLMIVFFLVLAGSFEGLSPGSLDVFLLIVLFTLAGSVFF
                     LHRLHHEEFDPARVSSAGTVLYERPWGRGRVILVGTSRGKLVAHLPPSTIFREGDEVD
                     IDGVIEPFGRSSEEGSFDEARFWKARGAVGKIRRPDIRLTRDNPGGILWLRRNLREKI
                     LLNLPPLMRGHLLAVILGGRDPDLAEKHSEWGTSHILAVSGLHAGVVVLVIFLLVPPG
                     PLRIPAASLAMWIYILLAGASVSALRAGLMIQAGFTGRWLGRPPSAVNAVSVAGLILL
                     FWRPWFFWDLGWRLSMTAALVLSCLFDGGARKGWVAAPVMLWLATAGMVTGVFGAVPV
                     AGTIINILAVPFFSLLIPLAFFLAIPTLAGIPGGRVLTEAGEGIFYIWAGIADRATSL
                     LPWAVSHSWVLVFSGTTLTSYLLLAGIGFRRSHAAVFSVSIAIFLTLL"
     gene            2770..>2992
                     /locus_tag="PK146_06210"
     CDS             2770..>2992
                     /locus_tag="PK146_06210"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPE91868.1"
                     /translation="MLLVVDIGNTNTVLGIFDGRELIRHWRLISERHTSDELGIYLLN
                     LISLAEVTPADISGAVLCSVVPPLDAPWEE"
BASE COUNT          572 a          795 c          912 g          713 t
ORIGIN      
        1 ggatcagata tgccacgtcc catccgaggg accttacccg ggatgtggtg gaggtaatgt
       61 ccgaacacga aaatatctgt cccgctatca accttcccat acagtcgggg agcgaccggg
      121 tccttgggct gatgaacaga gggtacacag tagggcagta ttgttcgata attgatatga
      181 taaggtctat cctgcccgga ccggctgtta caagcgacct gatagtcggc tttccgggag
      241 aaacctctga ggatttcaag gggtcggtca gggtcgttga agatcttcgc tttgacctgg
      301 tccacaccgc cgccttttcc ccccgggaag ggactccggc agcccggatg gaagctcagg
      361 tcgaggcgga ggagaaacag cggaggctca gggttatcaa ctcgatccag tccgtgatat
      421 ccagggagat caaccagggt cttgtcggga aggtcttcac agtcctggcc gacggtaacg
      481 ccccgaaggg agagggcctt cttcaaggca ggacacccac agacaaggtg gtcatcttcc
      541 ctggtgatct ttccatgatg ggttctttcg tcgatctaag gatcactgcc gcggggaact
      601 ggcatctgtt cggggagccg gtacccccct cctggtatga tcgaagggag tgatgggatc
      661 ccatggagat ccctggttcc agaaaaggat tgttgtttct tgcaggaggg gtactctgcc
      721 tggtcctggc agggtttctt gtagctacat tctccggtaa atgggacaaa agcccctccg
      781 ggcaggtggc tggtcctgca cctgtgtcat ccgcgagagc ctcttcgacg tcggggagtc
      841 ccggaggacc ggacgctccg cagttgcagg gggtcagcga tggccaatgg gtgatctata
      901 ttaccggagc tgtgatgagg ccaggggtat accggctgcc tgccggaacc agagtctacc
      961 aggcggtgga acaggcaggc ggcctgagtt ccgaggcaga ccccgaagcc ttcaacatgg
     1021 cccttcccct ttcagacgga gttcacgtgc atgttccccg caagggcgat cctcccggcc
     1081 agccggctgg ttaccagacc aatgatcatg ggcccgttca aggtcttgtc gacataaaca
     1141 gagcagactt ggcgactctt caacaccttc ctgggatagg cccaaagatc gccgatgaga
     1201 tcattcggtt cagagaggag cggggaagat tttcaagcgt agaggatctt ctttccgtga
     1261 aggggatagg agagaagaag ctctcggcaa taaaggacct ggttacggtc tcaccctagg
     1321 tcctttatcc agggctcctg ccttcgtggc ccttgcggga tcatgcatcc ttcttttcct
     1381 ggacgggagg gggttcccaa tcccggtgtc ggtcggggcc tcccttatga tcgtgttctt
     1441 tctggtcctg gcagggtcat tcgaaggttt gtcgccgggg agcctggatg tctttcttct
     1501 gatcgtcctc tttacacttg caggttctgt cttttttctc catcgtctcc accatgaaga
     1561 attcgacccg gccagggtgt cttccgcagg gactgttctg tatgaaaggc cctgggggag
     1621 gggaagggta atacttgtcg gaacttcgag ggggaagctg gttgcccatc tccccccctc
     1681 gactatcttc cgggaaggcg acgaggttga tatcgacgga gtgatcgaac ccttcgggag
     1741 gtcttccgag gaagggagtt tcgacgaggc ccgtttctgg aaagctagag gtgccgttgg
     1801 aaagataaga cgtcctgata tcaggctgac ccgggataac cccgggggca ttctttggct
     1861 gaggagaaac ctgagggaaa agatactcct caacctcccg ccactgatga gagggcatct
     1921 tctggcggtg atcctgggtg gcagagaccc cgacctggcc gagaagcact cagaatgggg
     1981 gacatcacac atcctcgcag tctctggtct ccatgccggt gttgtggtgc tcgttatatt
     2041 tctgctcgtt cctcctggcc cgttgaggat cccggcagct tctctggcca tgtggatata
     2101 catccttttg gcgggagcct cggtcagcgc actcagggcg gggttgatga tccaggccgg
     2161 ctttaccgga aggtggttgg gaagacctcc ttcggctgtt aacgccgttt ccgttgcggg
     2221 gctgatcctc cttttctggc gtccctggtt cttttgggat ctggggtgga ggctgtcgat
     2281 gacagctgcg ctggtcctct cgtgcctctt tgacggcggg gcaagaaagg gctgggtagc
     2341 ggcgccggta atgctctggt tggccacggc aggtatggta accggtgtct tcggagccgt
     2401 gcccgtcgct ggcacgatca tcaatatcct ggcagtcccc tttttcagtc tccttattcc
     2461 tctggccttc ttccttgcaa tacccaccct ggcggggata cccgggggaa gggtcctcac
     2521 ggaggcagga gagggtatct tctatatctg ggcaggcatc gcggacaggg caacctcctt
     2581 gctaccatgg gcggtttccc attcatgggt gctcgtcttt tcagggacga ccctgacgtc
     2641 ttacctgctt cttgcaggaa taggtttcag gaggtcacac gccgctgttt tttcggtatc
     2701 tattgccata ttcctgactt tgctttagaa agaagcctgg tatgaagtgg ataaacagga
     2761 ggtgggacca tgctccttgt agtggatata ggcaatacca acactgttct cgggatcttt
     2821 gacggaaggg aactgataag gcactggagg ctcatttcgg agagacacac ctctgatgaa
     2881 ctgggcatct accttctcaa cctgatatcc cttgccgagg tcacgccggc cgatatttcg
     2941 ggtgccgtcc tgtgcagcgt ggtacctcca cttgacgccc cctgggagga gg
//
LOCUS       DAOPKQ010000177         2978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_37552_length_2978_cov_2.971262, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000177 DAOPKQ010000000
VERSION     DAOPKQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37552_length_2978_cov_2.971262"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PK146_06215"
     CDS             <1..746
                     /locus_tag="PK146_06215"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPE91869.1"
                     /translation="LGPSRDQGIFFALPGGLFPMQVAECDGSARGTVLTFPDGSVICT
                     VEHLLGVLSGLGVWSAEINVRGPEVPALDGSGAEFAGAIFEASIPGEKIEPIDISREI
                     AFADPSRGGFISAIPSERFEVSYVINYDARGVGCQAFDGEINPPSFLKDIARARTFVL
                     ESEVKGIIERGLGKGGARENVLVIEDSAPPDPRELRLQDEPVRHKVLDLLGDLAILGL
                     PLKGRVMAYRAGHGMHLELVRRIRRSISA"
     gene            766..1212
                     /gene="fabZ"
                     /locus_tag="PK146_06220"
     CDS             766..1212
                     /gene="fabZ"
                     /locus_tag="PK146_06220"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPE91870.1"
                     /translation="MLDIHKIFGSLPHRYPFLLVDRILEIDSERVIGLKNVTINEPFF
                     QGHFPAEPVMPGVLILEAMGQVAGMLITQRPDMAGVTIYLTSVEKAKLRRPVVPGDQL
                     VTEARIERIRGRVGKVRTVARVGDEVAAEAVLGFILAPRLTGEKAE"
     gene            1209..2015
                     /gene="lpxA"
                     /locus_tag="PK146_06225"
     CDS             1209..2015
                     /gene="lpxA"
                     /locus_tag="PK146_06225"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPE91871.1"
                     /translation="MSEFYVHPSSIVSKKARIGEGAWIGPFCMVGSDVEIGERTRLEG
                     YVRLLEHVRIGKSCRICENVVLGGEPQDHAFKGERSFVVIGDGVILRENVTVHRGSGA
                     DSVTSIGSGTWMMVGSHAGHNSTVGENCILANGVALAGYSSVGDGSVLGGMSGLHQFV
                     RVGRYCMIGGLTKVVRDIPPFTLADGHPARIYGLNVIGLRRNGFSQEQRLAVKRTYRD
                     LFDPSSLMKESVARLEGNTPDDPNVIEILDFVRNSRRGITPWGERRERDD"
     gene            2008..2487
                     /locus_tag="PK146_06230"
     CDS             2008..2487
                     /locus_tag="PK146_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPE91872.1"
                     /translation="MIKLFVMDVDGTMTDGAVYLDGNCGEFKRFDVRDGLGISMLLRQ
                     GIGAAIISGRKSAVTSRRAEELGIDRVLQGIKDKMKALRQMSEEISLDPSEIAYMGDD
                     ANDAECLEWAGMGFAPSDSRREALDAADVIVPSRGGYGAVRDAVEIILSGKVKEAAE"
     gene            2484..>2978
                     /locus_tag="PK146_06235"
     CDS             2484..>2978
                     /locus_tag="PK146_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPE91873.1"
                     /translation="MRSGKKESKKKIARKGLWTARILDRFIVTQMGGSFLFGVMVFSL
                     LLVAGDLLFQIANLVIDKGVSLGVVLRLFVYKLPEVVVMTLPMASLLSALLTFGRLSS
                     QSEIVALRAAGISFSRIVRPVLAASIFISLFAMLLNETLVPLSNRAADNIMRYEVARE
                     RPSLL"
BASE COUNT          629 a          772 c          956 g          621 t
ORIGIN      
        1 ccctggggcc ctccagggac cagggaattt ttttcgctct ccccggaggg ttgttcccga
       61 tgcaggtggc ggagtgcgat ggcagcgccc gggggacggt gctgaccttt cccgacggga
      121 gcgttatctg caccgtcgaa catctgcttg gtgtcctgtc gggcctcggc gtctggagcg
      181 cggaaataaa cgtcagaggg cccgaagttc ccgccctcga cggaagcgga gcggaatttg
      241 ccggggccat atttgaggcc tccattccgg gtgaaaagat tgaaccgata gatatttcgc
      301 gcgagatcgc ctttgccgac ccctcgagag ggggatttat ctccgcgatc ccgtcggaaa
      361 ggttcgaggt aagttacgtg ataaattacg atgccagggg tgtgggctgc caggcctttg
      421 acggggagat caatccccct tcatttttga aggatattgc cagggccagg acctttgtcc
      481 tcgagtccga agtcaagggg ataatagaga ggggactcgg aaagggcggg gccagggaga
      541 atgtacttgt aatagaagat tccgctcccc ccgacccccg tgaacttcgt cttcaggatg
      601 aaccggtcag acacaaggta cttgaccttc tcggcgacct tgcgatcctc ggtctgcccc
      661 tgaagggtag agtgatggcc tacagggccg gacatggaat gcacctggaa ctcgtgcgga
      721 ggataagaag gagtatctcc gcctgaaacc gaaaggagaa atataatgct tgatatccac
      781 aagatctttg gatcgctgcc ccacaggtac cccttcctgc tcgtcgacag gatactggag
      841 atagacagtg aaagggtgat cggcctcaag aatgtcacga taaatgaacc cttcttccag
      901 gggcactttc cggcggagcc ggtaatgccc ggcgtgctta ttctggaggc catgggccag
      961 gtggccggga tgctgataac acagcgcccg gacatggctg gtgtgaccat atatctcact
     1021 tccgtggaga aggcgaaact gaggcggcca gtcgtccccg gcgaccagct ggtcacggaa
     1081 gcaaggatag agaggataag gggtcgcgtc ggcaaggtcc gtaccgtggc gagggtcggc
     1141 gatgaggtag ccgccgaggc ggtgctggga ttcatcctgg ctcccaggct aacgggagag
     1201 aaggctgaat gagcgaattt tacgtgcacc cctcttcgat cgtgtcgaaa aaggcgcgca
     1261 taggtgaggg tgcctggata ggacctttct gcatggtagg atcagatgtc gagataggtg
     1321 aacggacccg ccttgagggt tatgtacgcc tcctggagca tgtgaggata ggaaaaagct
     1381 gcaggatctg cgagaacgtg gttctcggcg gcgagcccca ggatcacgcc ttcaaggggg
     1441 agcgttcctt tgtcgtgatc ggcgacggcg tcatcctccg cgagaacgtc accgtccaca
     1501 ggggtagcgg cgcggattcg gttacctcca tcgggagcgg gacctggatg atggtcggtt
     1561 cccatgctgg gcacaactcg accgtaggtg agaactgtat cctcgcaaac ggggttgccc
     1621 tcgcgggata ctcctccgtc ggagatggtt ctgttctcgg gggcatgtcg ggccttcacc
     1681 agttcgtaag ggttggccgc tactgcatga tcggtggcct gacgaaagtg gtgagggaca
     1741 tccctccctt caccctggcg gatggtcacc cggcaaggat ctacggtctc aacgtgatcg
     1801 gccttcgaag gaacggcttc agccaggaac agagactggc ggtcaagagg acctacaggg
     1861 acctcttcga cccttcctcg ctgatgaaag agtccgtcgc aaggctggaa ggcaataccc
     1921 cggatgatcc caacgtaatt gagatactgg attttgtccg gaattccaga agagggataa
     1981 ctccctgggg cgagaggcgg gaaagggatg attaaactct tcgtcatgga tgtggatggc
     2041 accatgaccg acggggcggt ctacctcgac ggcaactgtg gggagttcaa gaggtttgat
     2101 gtccgcgatg gtcttggcat ctccatgctt ctcaggcagg gtatcggggc agcgatcata
     2161 agtggaagga agtcggcggt cacctcccgc agggcggaag aactgggcat agacagggtt
     2221 ctccagggga taaaagacaa aatgaaagcc ctgaggcaga tgtcggaaga aatttccctc
     2281 gacccgtcgg agatagccta catgggggac gacgcgaacg atgccgaatg cctggagtgg
     2341 gcggggatgg ggtttgcacc ctcggactcc cgcagggagg cccttgatgc cgccgacgtg
     2401 atcgttcctt caaggggcgg ctacggggct gtccgtgatg ccgtggagat catcctttcc
     2461 ggcaaagtga aagaggccgc cgaatgaggt ccggaaagaa agaaagtaag aagaaaatcg
     2521 ccagaaaggg gttatggact gcccggatac tggacaggtt cattgtcacc cagatgggtg
     2581 gatcattcct cttcggggtg atggtcttct ccctgctcct ggtcgcgggg gacctcctct
     2641 tccagatagc caacttggtc atagacaagg gggtctccct cggggtagtt ctgaggctct
     2701 ttgtctacaa gttgcccgag gttgtcgtca tgacccttcc gatggcctcg ctcctttcgg
     2761 ccctgctgac ctttggccgg ctctcttccc agagcgagat agtggccctc cgggcggcag
     2821 gaatatcttt cagcaggata gtccggcctg ttttggcggc gtcaatattt atctctcttt
     2881 tcgcgatgct tctgaacgag acccttgttc cgctgagcaa cagagccgcc gacaatatca
     2941 tgcgttacga ggtggcaagg gagcggccct cactcctg
//
LOCUS       DAOPKQ010000178         2966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_37756_length_2966_cov_5.345242, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000178 DAOPKQ010000000
VERSION     DAOPKQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37756_length_2966_cov_5.345242"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /gene="guaB"
                     /locus_tag="PK146_06240"
     CDS             <1..1249
                     /gene="guaB"
                     /locus_tag="PK146_06240"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPE91874.1"
                     /translation="NMSWEKQVAEVDKVKRSESGVIVDPFYLHPEDLVKDAVELMDHY
                     HISGVPIVDDRRRLVGIITNRDLRFVTDYQQPISGVMTHENLVTAPVGTDLEGATKIL
                     MKHKIEKLPIVDRDGILKGLITIKDIQKARDFPQASKDSRGRLRVGAALGVGEGALER
                     AANLVEAGIDVLVVDTAHGHSRSVMETVGLVRRNFPGVALVGGNIATGEAAEALINAG
                     ADAVKVGVGPGSICTTRVIAGIGIPQLSAVMRVAKVAHSRGKTVIADGGIRYSGDVVK
                     AIAAGADSVMIGSLFAGTDESPGEVVIYRGRSFKSYRGMGSLGAMKGGESKDRYFQEG
                     QEEEKLVPEGIEGLVPYKGPMSAIAYQLVGGLRSGMGYVGASNIERLQEDANFVKITG
                     AGVKESHPHDVVITKEAPNYRME"
     gene            1489..2016
                     /gene="rimP"
                     /locus_tag="PK146_06245"
     CDS             1489..2016
                     /gene="rimP"
                     /locus_tag="PK146_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPE91875.1"
                     /translation="MTRQVEREAVHRMHGSIRELVEESGYEYVWSGLALEEGRTVLRV
                     LIDSLGGINVKDCEIVSRKLGRMLDDSDPGLPDRYFLQVSSPGIERPLFKISDYERFS
                     GKRAKIRLKSGLDGRKNLTGTVISVSGETVLFREDGSGEISVPFNDISRANLVFEMPG
                     PEKPVAKGKKKRGQQ"
     gene            2016..>2966
                     /gene="nusA"
                     /locus_tag="PK146_06250"
     CDS             2016..>2966
                     /gene="nusA"
                     /locus_tag="PK146_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPE91876.1"
                     /translation="MQLGRDFVRALKQITQERNLPLEVISSGLEAAMISAYRKYRGGD
                     QNIEVFVALESGEVSICEVRQIVEVVNDPDTELSMEEASKLGFDDVSFGDIVRVEVEP
                     EKFGRIAAQTARQVIIQRLKDAERQVIYEEFSDKIGDLVNGVVFKSENDQVLVRLNDR
                     TEAILPREERIVGESYSLGSRMKFFLVEVRQTTRGPKIVVSRSHPGLLRKLLELEIPE
                     IREGTIEIREIVRESGARAKVAVSTLDPNVDPVGACIGNAGARIKSISKELGGERIDV
                     VVWSSDPLVFIRNALSPARVLKVEPVLEQERSVQVFVRPDH"
BASE COUNT          741 a          696 c          939 g          590 t
ORIGIN      
        1 gaatatgagc tgggaaaaac aggtcgccga ggtcgacaag gtcaagcgct ccgaatcagg
       61 ggtcatcgtg gaccccttct acctccaccc cgaggacctg gtcaaggatg cagtcgagct
      121 catggaccac taccatatat cgggagtacc aattgtcgac gacaggcgta gactggtagg
      181 gataataacc aaccgtgacc ttcgtttcgt taccgactac cagcagccca tcagcggggt
      241 tatgacccat gagaacctgg tcacggcacc cgttggcacg gatcttgaag gcgccaccaa
      301 gatactgatg aaacacaaga tcgaaaaact ccctatcgtc gaccgtgacg ggatcctgaa
      361 aggtctgatc accatcaagg atatacagaa ggccagggac ttcccccagg catcgaagga
      421 ctccagggga agactcaggg tcggtgctgc gcttggtgtc ggggagggag ccctggaaag
      481 ggcagccaat ctggttgagg cgggcattga tgttcttgta gttgatacgg cccatggaca
      541 ctccaggtcc gtgatggaaa cagtaggact tgtcaggagg aacttccccg gagtcgcgct
      601 tgttggagga aatatcgcaa ccggagaggc ggccgaagcg ctcatcaacg ctggagcgga
      661 tgcggtcaaa gtgggggtcg ggcccggatc gatatgcacc acacgggtta ttgcgggaat
      721 cggcatcccc cagctttcag cggtcatgag agttgccaaa gtggctcact ccagaggcaa
      781 gacagttatt gccgacgggg ggataaggta ctcgggagac gtggtcaagg ccattgcagc
      841 cggagctgat tcggtaatga taggatccct tttcgccggt acggacgaga gtcccggaga
      901 ggttgtcatc taccggggaa ggtccttcaa aagttacagg gggatgggct ccctgggggc
      961 gatgaagggc ggcgagagca aggaccgcta tttccaggag ggccaggaag aggagaaact
     1021 ggtcccggaa gggatcgagg gactcgtccc ctacaaaggc ccgatgagcg ctattgcata
     1081 tcagcttgtg ggtggtctga gatcaggcat gggttacgta ggcgcctcca atatcgaacg
     1141 gctgcaggaa gatgccaact tcgtcaaaat aaccggcgct ggtgtcaagg agagccatcc
     1201 ccacgatgtg gtgataacca aggaggcccc gaactaccga atggaataga tatggaaagg
     1261 ctggaaagtc ggcgaacctg gtcccgaacg aagggtcttt caatcacaac cgtgtcgctg
     1321 tcggttctcg gaacaagttg aaaaaagtct tgagggcgtg gtatactcat caaagttgaa
     1381 ccgccttcaa agttctggat ggggcgaaga gcagggagat cctgctcttc gttttttatc
     1441 cgctcttaaa acaggtttaa cagttttgta gcaggtggtg ctttgagaat gacaagacag
     1501 gtcgaaagag aggctgtgca caggatgcat ggttccatca gagagctggt cgaggaatcc
     1561 ggttatgaat acgtctggtc cggactggca ttggaggagg gccgtacggt tctcagggtg
     1621 ctgatcgaca gtctgggcgg cattaatgtc aaggactgcg agatcgtgtc gagaaagctg
     1681 ggaagaatgc tcgacgacag tgaccctggt ttgcctgata gatacttcct gcaggtcagt
     1741 tccccgggga tcgagagacc ccttttcaag atctctgact atgaacgttt ttcggggaag
     1801 agggcaaaga tacgactcaa aagcggtctc gatggacgaa agaatctgac aggaactgtt
     1861 atttccgttt ccggggagac tgtcctgttc cgcgaagacg gatcggggga gatctctgtc
     1921 cccttcaatg acatttcaag agcgaacctg gtctttgaaa tgccgggccc ggaaaagccc
     1981 gtagccaagg gaaaaaagaa aagggggcag cagtaatgca gctgggacgc gatttcgttc
     2041 gagccctgaa gcagataacc caggagagga acttgcccct ggaggtcatt tcctcgggcc
     2101 tcgaggcggc gatgatatcc gcctaccgaa agtaccgggg gggagatcag aatatcgagg
     2161 tcttcgtggc cctggagagt ggggaagtat ccatctgcga ggtcaggcag atagtggaag
     2221 tggtgaacga tcctgacacg gagctgtcca tggaagaggc atcaaaactt ggcttcgatg
     2281 acgtttcttt tggagatata gtcagggtgg aggtcgaacc ggagaaattc ggtcgcatag
     2341 cagcccagac agcaaggcag gtcattattc agaggctgaa ggatgccgag aggcaggtca
     2401 tatacgagga gttctcggac aagatcggcg atcttgtcaa cggggtggtg ttcaagtccg
     2461 agaacgacca ggtcctggtg aggctgaacg acagaaccga ggccattctg cccagggaag
     2521 agagaatagt cggggaatcc tattctctcg gaagcaggat gaagttcttc ctcgtagagg
     2581 tgagacagac gaccagggga cccaagatcg tcgtctcccg gagccatccg ggacttttga
     2641 ggaagcttct tgagcttgag atccccgaga tcagggaggg gaccatcgag atcagggaga
     2701 tagtaaggga gtctggcgca agggccaagg tagccgtatc cacccttgat cccaacgtgg
     2761 atcccgtagg ggcctgtatc ggtaacgcag gtgccaggat caagtccata agcaaagagc
     2821 tcggcggcga aagaatagac gtggtcgtat ggagcagcga tcctctggtc tttatcagga
     2881 acgccctttc gccggcccgg gtcctgaagg tcgaaccagt tcttgaacag gagagatccg
     2941 tccaggtgtt cgtcaggcct gatcac
//
LOCUS       DAOPKQ010000179         2900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_38947_length_2900_cov_3.781019, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000179 DAOPKQ010000000
VERSION     DAOPKQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38947_length_2900_cov_3.781019"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1216)
                     /gene="lysS"
                     /locus_tag="PK146_06255"
                     /pseudo
     CDS             complement(8..1216)
                     /gene="lysS"
                     /locus_tag="PK146_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163402.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
     gene            complement(1170..2249)
                     /locus_tag="PK146_06260"
     CDS             complement(1170..2249)
                     /locus_tag="PK146_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPE91877.1"
                     /translation="MRWTEGRTEISFDAGSFPAKVGGVDVENPVWLAPLAGITIPPVR
                     SFFRRLGAGLVHTEMVSCAGLVHGSRKTFSMLRTADGERPVVLQLFAADTENLLEGAR
                     TALDRSSFEALGVNMACPMPKVTRRGAGSGLLERPEIAAAMVKALVRLGLPVWVKIRK
                     PPMTSPMDTLAFSGMLLEAGASHVCIHGRQPSQKYGGKADSSVVLGAARAFPGRIAAS
                     GDIFTPGDAKRMLDGGCSAVFVARGAFRDPFLVPEILNHCGFPVDPGNLADSPVSRMD
                     LMISLGERILSEEGEKAAIVLLKRFLSGIFRGLYGASRFRQAVSRSSSWGEMRAALEE
                     WKASPERGEQCDRRETFRRDRSPGG"
     gene            complement(2224..>2900)
                     /locus_tag="PK146_06265"
     CDS             complement(2224..>2900)
                     /locus_tag="PK146_06265"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPE91878.1"
                     /translation="HTSDELGIYLLNLISLAGVTPAEISGAALCSVVPPLDAPWEEGI
                     RRYLSVECLKVTNRTNIGIPVLYENPSEVGADRLVNAVAGIECFGSPLLVVDFGTAIT
                     IDAISTRGEYLGGSIAPGLVVSMEALFGKAARLPKVSFDLPEKVIGRNTRDSMQSGFL
                     YGFAGMVDSLVERIREEMGEEVRAIATGGQAEAVAPVSRTISSVEPWLTLEGLRLIYE
                     MDRGEN"
BASE COUNT          581 a          893 c          795 g          631 t
ORIGIN      
        1 cagcctacct ttgtgccagg ggagagatct ccgtagggag ccctagcaca aaggtaggct
       61 ggataaggga atcctccacg aactcatcga agagtttcgt caggaccagg aagcggctct
      121 cgtaaccctc catgtccact cccttctctt tggcaaaggc acgggccttc tcatcgtcct
      181 ccttcccgta ctggtagata tcgaaaccgg tcctctccct gacaagttct gccatggtgg
      241 cacgcctgaa gggggggtga aaactgacag gcgttccctg gtagaccacg tccctgctgc
      301 ccacggaatc agcggctgcg actatcagct cctcggtgag gtccattatg tcctggtagt
      361 ctgcataggc ccagtagacc tccatggagg tgaactccgg gttgtgcatt atgtctatac
      421 cctcgttgcg aaagttcttg ccgatctcgt agacccgccc cagcattccc acaatgagcc
      481 tcttgaggta tagctccgtg gctatcctca tgtacatgtt gatgccgaga gcgttatgaa
      541 aggtaacgaa ggggcgggcg ttcgccccac cggcaagggt ggccagtatc ggagtctcaa
      601 cctccagcga cccatgggcc tcgaggacac tcctgaaggt ggagattatt ctggctctct
      661 tcctgaaagt ctcacgtacg tcggggttcg cgatcaggtc cacgtacctc tggcggtatc
      721 tggtctcggt atccttgagg ccgtgccatt tctcaggaag ggggcgaaga gccttggcga
      781 gcaggacaca cctggtgacc aatatggaca gctcgcccct tctggtccgg aaggggtgcc
      841 cgactatgcc gagcatatca cctgtatcaa cccatttctt gaagaagccg tactcctctt
      901 cacccatggc atctacctga aagtagagct gcaagctgga ggtctcgtcg gcaaggttgg
      961 caaaggccgc cttgccgtgt ctcctgaggg tcatcaggcg cccggcggtc actatctccg
     1021 tatcgtccct ttcatcctcg gcaaggtggc tgaaattttt cttcaccaga tcgagggtgt
     1081 gtttctttat ccacttctca tggacgaagg ggtcgtaccc ctcctcctcg cgtagtctta
     1141 tcagcttgtc ggtgcgctgt ctgaggatct catcctcctg gacttcgatc tcttcggaat
     1201 gtctctcttc gatcacactg ctcgcccctt tcaggtgatg ctttccactc ttcgagcgcc
     1261 gccctcattt ctccccagct ggaagatctg gagacggcct gcctgaaccg tgaagctccg
     1321 tataaccctc taaagatacc cgagaggaag cgcttgagca agacaatagc cgctttttct
     1381 ccttcttcgc tcagtattct ttccccaagg gagatcatca ggtccatcct ggaaaccggg
     1441 gagtccgcca gatttcccgg atcgaccggg aaaccgcaat gatttagtat ttccgggacc
     1501 aggaagggat cacggaaggc ccccctggca acgaatacgg cggaacagcc cccgtccagc
     1561 atcctctttg catccccggg ggtgaagatg tccccgcttg ccgcgatcct tccgggaaat
     1621 gcgcgcgcgg ccccaaggac cacgctgctg tcagccttgc ccccgtactt ctgggaaggt
     1681 tgtctgccat ggatacagac atgggatgcc ccggcttcaa gaagcatccc cgagaaggcg
     1741 agggtgtcca ttggagatgt catgggtggt ttccttattt tcacccatac aggaagacct
     1801 agccgaacaa gggctttgac catggcggcg gcgatctcgg gacgttcgag aaggccggag
     1861 cccgcgcctc ttctggtcac cttgggcatg gggcaggcca tgttcacccc caaggcctcg
     1921 aaagaggaac ggtccagtgc cgtccgggcc ccttcaagga ggttctccgt atcggcagcg
     1981 aagagctgga gcacgaccgg tctctcccca tcggccgttc tgagcattga gaaggtcttt
     2041 ctgctcccgt ggacaagacc ggcacagctg accatttcgg tatggaccag gcctgcgccc
     2101 agccgcctga agaaggaccg gaccggtggg atcgtgatcc ccgcgagagg ggcaagccag
     2161 acggggttct ccacatccac tccgcccact tttgcaggaa aggagccggc atcgaaagag
     2221 atctcagttc tcccctctgt ccatctcata gatcagcctc agcccttcga gagtaagcca
     2281 gggctccacc gagctgatcg tccttgaaac aggtgccact gcctctgcct ggcctcctgt
     2341 agcgatggcc ctgacctctt ctcccatctc ttccctgatc ctctccacca gcgaatccac
     2401 cataccggca aagccataga gaaaaccgga ctgcatcgag tctctcgtgt ttctccctat
     2461 gaccttttcg ggcaggtcga aggagacctt gggcagcctg gctgccttcc cgaagagggc
     2521 ctccatgctc accacgagcc ccggcgcaat cgaaccaccc agatactccc cgcgggtcga
     2581 tatggcgtcg atcgttatgg ctgttccgaa gtccaccacc agaagggggg aaccgaaaca
     2641 ttcgatcccg gccacagcgt tcacaaggcg atcggccccc acttcggagg ggttttcgta
     2701 caggactgga atgccgatgt tggtcctgtt ggtgaccttc aggcactcaa cggaaaggta
     2761 gcgcctgatc ccctcctccc agggggcatc aagtggaggc accacgctgc atagggcagc
     2821 acccgaaatt tcggccggtg tgaccccggc aagggatatc aggttgagaa ggtagatgcc
     2881 cagttcatca gaggtgtgtc
//
LOCUS       DAOPKQ010000180         2870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_39539_length_2870_cov_4.049023, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000180 DAOPKQ010000000
VERSION     DAOPKQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39539_length_2870_cov_4.049023"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..579)
                     /gene="hslV"
                     /locus_tag="PK146_06270"
     CDS             complement(46..579)
                     /gene="hslV"
                     /locus_tag="PK146_06270"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPE91879.1"
                     /translation="MMKGTTILCVRRNNVVAMAGDGQVTLGDQVIKEGARKVRRLYDG
                     RVLTGFAGGTADAMTLLERFEKRLAEHGGNLIRSATELVKDWRTDKVLRRLEAMMLVA
                     DSRSTLLLTGAGDILEPEGEACSIGSGAGYALAAARALLESSDARADEIARRSLVIAS
                     RICIYTNDSITLEVLED"
     gene            complement(539..1471)
                     /locus_tag="PK146_06275"
     CDS             complement(539..1471)
                     /locus_tag="PK146_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPE91880.1"
                     /translation="MPGDAISESIDLFLEEMRSGRGGSGHTTLNYAVDLSQFADYLEG
                     LGVTAPGEVQSLHIRGFLRDVVGFGYARSSAARKLSAIRSWLRFLRERREIPSNPAEG
                     IRGPRLPGRLPRAISREDMQKLLETGPSGPEAHRDLASMELMYGSGLRVAETASLRWD
                     QVDLEERWIRVMGKGEKERLVPMGRYSVKALREWRDSCPVVSDYVFPGSRGEHITVRT
                     LHRSVLKAAARAGLSGVTPHVLRHSFATHMLEGGANLRVLQELLGHESLVTTQKYLKI
                     TVEQLRKSYTMAHPRAGSGDADDEGDNDPLRQEE"
     gene            complement(1500..2846)
                     /gene="trmFO"
                     /locus_tag="PK146_06280"
     CDS             complement(1500..2846)
                     /gene="trmFO"
                     /locus_tag="PK146_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPE91881.1"
                     /translation="MTVVGAGLAGSEAAWQLCRRGIPVTLIEMRPHRTTPAHRTGGFA
                     ELVCSNSLGAFEITSPGGILKAELDMMGSLILDCARRSRLPAGKALAVDRDSFSECIT
                     GALASDPLVAVERKEIHEIPEGPAIIATGPLTSDPLARSLSRTVGENFLSFFDAVSPV
                     VTAESIDMTRAFRGGRWGFGDDYINCPMNREEYEAFHAALVSAERAPRHDFEKDDRYF
                     EGCLPVEVIASRGVDTLRFGPLRPVGLSDPISGEEPWAVVQLRQENTSGTLYNLVGFQ
                     TNLRWPEQESVFRMIPALSGAEFVRLGVMHRNIYVDAPKVLDPFLRLKVQSDIFLAGQ
                     ITGVEGYVESTAMGLVAALNTAALVSGQEFPVWPRETAVGSLLAHLSDPQPRSFQPMN
                     TNLGIFPPLLQRIKKRPERCGKYASRSSESMKKFIEELPPLLRKIPTIQISSEDYD"
BASE COUNT          549 a          898 c          813 g          610 t
ORIGIN      
        1 aggtaactat tgccttgggg gtaagggcct cgttccttcc ttccctcaat cctcaagcac
       61 ctccagcgta atcgaatcgt tggtgtagat acagatcctg gaggctatga cgagtgacct
      121 tctggcgatc tcgtcggccc tggcatctga ggactccagg agggccctcg ccgcggccag
      181 ggcatatcct gcacctgaac cgatggaaca ggcctcaccc tccggttcca ggatatcccc
      241 ggccccggtc agaaggaggg tagatctgct gtcggccacg agcatcattg cctccagcct
      301 tctcaggact ttgtcggtcc gccagtcctt gaccagttcg gtggcggacc ttatcaggtt
      361 gcccccgtgt tcggccagtc tcttctcgaa gcgttccagc agggtcatcg catcggccgt
      421 accccccgcg aaccccgtga gcactcgacc gtcgtagagg cgcctgacct tcctggcccc
      481 ttcctttatt acctggtccc caagggtgac ctggccgtct ccggccatgg ccacaacatt
      541 attcctcctg acgcaaagga tcgttgtccc cttcatcatc cgcatcaccg cttccggccc
      601 tggggtgggc catggtatag cttttcctca gctgctctac cgttatcttc agatacttct
      661 gggtggtcac aaggctttcg tgtcccagca gctcctggag gaccctcagg ttggctcccc
      721 cttcgagcat atgggtcgca aagctgtgcc tcagaacgtg gggggtaacc ccggagaggc
      781 cagccctggc cgccgccttc aggacgctcc tgtgaagagt tcttacggtt atatgctccc
      841 ccctgctgcc ggggaatacg taatccgaga cgaccggaca ggaatctctc cactccctca
      901 gggccttgac ggagtacctg cccatcggga ccagccgctc cttctcaccc ttgcccatta
      961 ccctgatcca tctttcttca aggtccacct ggtcccagcg aagggaggcc gtctctgcga
     1021 cccgcagccc ggagccgtac attagctcca ttgacgccag gtccctgtgg gcctccgggc
     1081 cggaagggcc cgtctccagg agcttttgca tatcctcccg ggagatcgcc cttggtagcc
     1141 ttccaggaag cctgggtccc cggatcccct cagcggggtt cgacgggatc tcgcgacgct
     1201 cccggaggaa acgaagccag ctccttatgg cagaaagctt tctggctgca gaggagcggg
     1261 cgtagccgaa gccaaccacg tccctgagga agccccttat gtgcaggctc tgcacctctc
     1321 cgggggcggt gacccccaga ccctccaggt agtcggcaaa ctgggagagg tccacggcat
     1381 agttgagggt cgtgtgtccc gaacctcccc tgccggacct catctcttca aggaaaagat
     1441 cgatagattc ggaaatagcg tccccgggca tgtcaataat tgtaacactt tggagaggat
     1501 taatcataat cttctgaaga tatctgtatg gtcggaattt ttctcaaaag aggtggtaat
     1561 tcttcgatga acttcttcat ggactccgag gacctggaag catacttccc acatcgttcc
     1621 ggcctcttct ttatcctctg gagcaacgga ggaaagatgc caaggttggt gttcataggc
     1681 tggaaggacc tgggctgggg atccgagaga tgagcaagca gggagccgac ggcggtctcc
     1741 cttggccaga cgggaaactc ctgccccgag accagggctg ctgtgttcag ggcagccaca
     1801 agccccatgg cggtgctctc aacatagcct tcaacgccgg tgatctgacc agcgaggaag
     1861 atatcgcttt gaacctttag cctgaggaaa ggatcgagaa ccttgggagc atctacgtag
     1921 atgttccggt gcatcactcc gagcctgacg aattcggctc ccgaaagggc cgggatcatc
     1981 ctgaacacgc tctcctgttc aggccaccgg aggttggtct ggaaaccgac gaggttgtag
     2041 agggtacccg aggtgttctc ctgacggagc tggaccaccg cccatggctc ctcgccggag
     2101 atcggatcac tgagacccac aggccggaga ggaccgaacc gcagcgtgtc tacccccctg
     2161 gaggctatca cttccacggg gagacacccc tcgaagtaac ggtcgtcctt ttcgaagtcg
     2221 tgcctgggag ccctctcggc agagacaaga gcggcgtgga atgcctcgta ctcctccctg
     2281 ttcatggggc agtttatgta atcgtcacca aagccccatc ggccgccccg gaaagccctg
     2341 gtcatgtcta tggactcggc ggttaccact ggagagacag catcgaagaa ggacaggaag
     2401 ttctcgccta ccgttcgcga aagagatctg gccaggggat cgcttgtgag gggacccgtt
     2461 gcgataattg ccgggccttc ggggatctca tggatctcct ttctttctac cgccacaagg
     2521 gggtctgaag cgagggcacc ggtaatgcat tccgagaagc tgtcccggtc cacggccagc
     2581 gctttcccgg ccggtagccg ggaccttctg gcacagtcaa gaatgaggct gcccatcatg
     2641 tccagttctg ccttgaggat gcccccgggg ctagtgatct caaaggctcc cagggagttg
     2701 ctgcaaacca gctccgcgaa tccccctgtc ctgtgggcag gtgtcgttct gtgggggcgc
     2761 atctcgataa gggtcaccgg tatgcccctc cggcacagct gccaggcagc ctctgaacct
     2821 gccaggccag ccccgactac ggttatcctt ctagtcccgg gatctttctt
//
LOCUS       DAOPKQ010000181         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_39596_length_2867_cov_4.715149, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000181 DAOPKQ010000000
VERSION     DAOPKQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39596_length_2867_cov_4.715149"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PK146_06285"
     CDS             <1..666
                     /locus_tag="PK146_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPE91882.1"
                     /translation="PVRDISGIFRFTARIGTVLPIVRRGNGGPMTVAVPLRNEKGEAL
                     QVKALLSAEQASEGPLVLSEWNLAMVIDRILGTPYGWGDLGGNRDCSGSLRDLFTAFG
                     VWLPRNSAAQARAFPFSSFGEVTGDQKEQRLVREGVPFATLLRVPGHIMLYLGTFRGV
                     PFVFHSTWGVRTFRNGEEGRHVIGSAVITTLRPGMDVECFDPSRGDLADRLSEMTFLL
                     PCR"
     gene            complement(663..2378)
                     /locus_tag="PK146_06290"
     CDS             complement(663..2378)
                     /locus_tag="PK146_06290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPE91883.1"
                     /translation="MWGKLIVLLSLAFQVAASVVSLRLFKVPRRHPCVFLIAGVMILM
                     VWRRLITFIHILSGNSLSLKETVFESLGLAISVFLFLSALWAVPRLMQFISEKMSSWR
                     SIQNASQIIRDLAIPAFVIDRDHTVIHWNRACEKLTGIPAEEMVGTRSPWRAFYNEER
                     PVLADMIVDQASAEDLARLYGRSSHPSSTVKDGWEAEFFLPALNGGRWLFFSAGPLYT
                     PEGEICGSIETFQDTTSKMKTGIHLSRSTSRLRTLHDIVKEISGEKTVKSLLDRAASL
                     LKEEMNISNVTFLENTADKRERDVRLRLAASSSLDADTMERLTIELPRSGLGLSTRAA
                     VSRKNVVTANVWEDPEYIPFFDGTFSEIDVPILDGEQVLGVISVEGYAPFDSQDEELF
                     NILAGHLASLWRSIELVSEVECLALTDQLTGLPNRHALFQRLCSEENRLLRYGGRMSV
                     LMMDMTRFKEINDTYGHMMGDDAMRASAACIRDCLRGSDFVARFGGDEFVALLPETGS
                     DEASLVVERISKRLSDVRVSGIPLDLRADFGLALFPDDGKKMTTVIQKADNRMYENKA
                     GRVDT"
     gene            complement(2519..>2867)
                     /locus_tag="PK146_06295"
     CDS             complement(2519..>2867)
                     /locus_tag="PK146_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPE91884.1"
                     /translation="SDLPELKRVIVSSGGKVAWAETVDEALSTLLLRLHGAGLPRVAP
                     PETAPGEERPAARLPVIVSGTARDLAGQARDHFDEAQRASRMGDWARYGEELEKLREV
                     LEALLEETVPPSQ"
BASE COUNT          576 a          812 c          828 g          651 t
ORIGIN      
        1 cccgtcaggg acataagcgg catcttcagg ttcaccgcac ggatcgggac agtgcttccc
       61 atcgtaagga ggggtaacgg aggtcccatg acggtggcag taccattgag gaatgaaaag
      121 ggcgaagcct tgcaggtgaa ggccctcctt tcagccgagc aagcctcaga gggtcccctt
      181 gttctttcgg agtggaatct tgcgatggtc atcgacagga tcctcggcac accctacggc
      241 tggggggatc tcgggggtaa ccgggattgc tcgggaagcc tgagggacct tttcaccgcc
      301 tttggagtct ggctcccccg gaactcggcg gcccaggcca gggccttccc cttcagttcc
      361 tttggagagg tcacgggaga tcagaaggag cagaggctgg tcagggaggg ggtacccttc
      421 gcgacgttgc tcagggtgcc ggggcacata atgctctacc ttgggacctt caggggggtg
      481 ccctttgtct tccactccac ctggggtgtc aggacattcc ggaatggaga ggaggggcgc
      541 cacgttatcg gttccgcggt tattaccacg ctcaggcccg gtatggatgt ggaatgcttc
      601 gacccttcca ggggggatct ggcagacagg ctctcggaga tgaccttcct gctcccctgc
      661 cgctaggtat cgacccttcc cgctttgttc tcgtacatcc tgttatcggc tttttgaatt
      721 accgttgtca tctttttccc gtcgtcggga aagagggcaa gtccgaagtc ggccctcagg
      781 tcaaggggaa tgccgctaac tctgacatcc gacagccttt tcgagatcct ttctaccacc
      841 aacgaggcct cgtcggagcc tgtctccgga agcaaggcca cgaattcatc tccgccgaac
      901 cgtgccacaa aatccgatcc ccgcagacaa tcccttatgc aggccgccga agccctcatt
      961 gcatcgtcgc ccatcatgtg cccataagtg tcgttgatct ccttgaaccg ggtcatgtcc
     1021 atcatcagga ccgacatcct tccaccgtag cggagaagcc tgttctcctc gctgcagagc
     1081 ctctggaaca gggcgtgcct gttggggagg cccgtcagtt ggtctgtgag agcaaggcat
     1141 tcgacctcac tgaccagttc aattgatctc cagagcgatg ccagatgccc ggcaaggata
     1201 ttgaacagct cctcgtcctg tgagtcgaag ggggcgtacc cctcgacgga tatgactccc
     1261 agaacctgtt ctccgtcgag gatggggacg tctatctcgg agaaggtgcc gtcgaagaag
     1321 gggatatatt cggggtcctc ccaaacattt gctgtaacca cgttcttcct ggaaacagcg
     1381 gctctggtgc tcagtccaag acctgatcgc gggagctcga tggtgagtct ctccatggtg
     1441 tctgcatcga gcgaactgga ggccgccaac cgaagtctga catcccgctc tcttttgtct
     1501 gcggtgttct cgaggaaggt gacgttcgaa atattcatct cctccttgag caggctggcc
     1561 gcccgatcaa ggagggattt tacggtcttc tcccccgata tctcctttac gatatcgtgg
     1621 agggtgcgga gtctggatgt gcttctggag aggtggatac cggtcttcat cttgcttgtg
     1681 gtgtcctgga aggtttcaat ggagccgcag atctctcctt caggggtgta gagtggcccc
     1741 gccgaaaaga agagccacct gcctccgtta agtgcgggca ggaagaactc tgcctcccag
     1801 ccgtccttca cggtagagga ggggtggctg gatctgccgt atagtcttgc gagatcctcc
     1861 gccgatgcct ggtcaactat catgtcggcc agcacaggtc tctcttcgtt gtagaaggcc
     1921 ctccagggtg atctggtgcc caccatctct tcggccggga tgcctgtcag tttctcgcag
     1981 gccctgttcc agtggatcac ggtatggtcc ctgtcaatga caaaggcggg tatcgcaagg
     2041 tcccgtatta tctgtgaggc attttgaatg cttctccagg agctcatttt ttccgaaatg
     2101 aattgcatca gtcgggggac tgcccacaag gcggacagga acaaaaagac cgatatggcc
     2161 agtccaaggg attcgaaaac ggtttctttt aaagagaggg agttacctga aaggatatgt
     2221 ataaaggtta tcagacgacg ccacaccatc aggatcatga ctcccgcgat gagaaagacg
     2281 cagggatgcc ttcgggggac cttgaaaagc ctgagggaaa ctacgcttgc ggcaacctga
     2341 aaagcaaggg agagaaggac tatcagcttt ccccacattc cgggaccgct cctttgggat
     2401 cacaaggtgt taaaagctta aagcttttat aaatgataat caatctcatt tcatgaatgg
     2461 ctcctttttc aaggatctcc agaggaaaag gggctccccg gcggggagcc ccaaatgatc
     2521 actggctcgg tggtactgtt tcttccagga gggcctccag gacctcacgg agtttctcca
     2581 gttcctcccc gtatctggcc cagtcgccca tgcgcgaggc ccgctgggcc tcatcgaagt
     2641 ggtctcttgc ctgtccggcc agatccctgg ctgtcccgct cactattacc ggtagacgtg
     2701 cggcgggtct ttcttctccg ggagccgtct ccgggggggc caccctcggc agtcccgcac
     2761 cgtggagccg cagcaggagg gtcgaaaggg cttcatcaac tgtctccgcc caggcgacct
     2821 ttccgcccga agagacgatc accctcttga gttcaggcag atcgctg
//
LOCUS       DAOPKQ010000182         2839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_40181_length_2839_cov_3.174928, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000182 DAOPKQ010000000
VERSION     DAOPKQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40181_length_2839_cov_3.174928"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PK146_06300"
     CDS             complement(<1..579)
                     /locus_tag="PK146_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPE91885.1"
                     /translation="MELRTIESNKLKGKKVLVRVDFNVPMDEGRVADDTRIRAHLKTL
                     ELLRRSGARIALVSHLGRPGGKVSPGLSLFPVARSLEDLTGWRIFFCEECVGDKISAT
                     IGSMGEGQICLLENVRFHPEEEANDPVFARKLGKPFDVFVMDAFSAAHRGHASTEGVT
                     RFLPSFAGKLMEKEVSVLSAVKENPKRPMVLIL"
     gene            complement(585..1601)
                     /gene="gap"
                     /locus_tag="PK146_06305"
     CDS             complement(585..1601)
                     /gene="gap"
                     /locus_tag="PK146_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPE91886.1"
                     /translation="MGMKRVAINGFGRIGRLALRALFEYENDGLFEIVATNSRTTAEQ
                     RAYLFKYDSVHRRFSGDVSVKGESLLINGKTVKVLAEAEVEDLPWKDMGIDLVIESSG
                     KYTDASKAAKHLEAGARKVLITAPAKNEDATIVMGVNEGIYDPSRHHVVSNASCTTNC
                     LAPVVKVIHEAFVIEKGLMTTTHAYTNDQKTLDASHKNLHRGRAAALSMIPTTTGAAS
                     AIGKVYPEVAGKMNGLAVRVPTPDVSLVDLVTVLSREVTSEEVNGALLKAAEGPMKRY
                     LAYEEDDIVSVDVTGDSRSSVFAARHTMTLGNLVKTLSWYDNEWGYACRVVDLANYML
                     SREA"
     gene            complement(1698..>2839)
                     /locus_tag="PK146_06310"
     CDS             complement(1698..>2839)
                     /locus_tag="PK146_06310"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPE91887.1"
                     /translation="TPMVKDLLHSASHCLSEMNGKRPKVFSGYPSVRESARETFVSVP
                     GLLLVTGRKGFAERLLRVWLERAVGNGHVMPSEVNPHTGALETRACDTGLWFFYALEK
                     FCDQSGQVELVRENWSDLAAVLENYRNGIPEIGISMDSDCLLELRSDDPGSNWMDGET
                     EGRPIVERRGKLVEVNALWYNSLRVMEKYSEIIGRESDGRDYGSVADECRRNFHKIFW
                     SEKGYLYDWVDGGTRDESIRCNQILAVSLPATVMDPERGRSVVETCWNELYTTFGLRT
                     LDPHHEKYKGRCEGRLDQREKARFMGMAWPWLLGHFITAYLRYNPDRKDIAWCFLRPF
                     LSHLRKGCLGGVAEIFDGSMPYKPHGDALYSPSVSELLRVIGEDM"
BASE COUNT          575 a          857 c          729 g          678 t
ORIGIN      
        1 caggatcagg accatcggcc gtttcgggtt ctccttgaca gccgatagga cgctaacctc
       61 cttttccatg agctttccgg caaaggatgg caggaatctg gtaactccct cggtggaggc
      121 gtggcccctg tgggcagcgc tgaaggcgtc cattacaaag acatcaaagg gctttcccag
      181 ttttctggcg aaaaccggat cgttggcctc ctcctcagga tggaacctga cattctccag
      241 aagacagatc tgcccctccc ccatcgatcc tatagtggca gagatcttgt cacccacaca
      301 ctcttcgcag aagaagatcc tccagcctgt cagatcttca agcgaccggg caacgggaaa
      361 gagggagagg cccggggaaa cctttccccc gggcctgcca aggtgggaaa caagggcgat
      421 ccttgcgccg ctcctcctga gcagttccag cgtcttcagg tgggccctta tgcgcgtgtc
      481 atcagcaacc ctgccttcgt ccatggggac gttgaagtcc acccggacaa gaaccttctt
      541 tcccttgagt ttattgcttt cgatggtcct aagttccatg tcgatcaggc ttccctggaa
      601 agcatgtagt tggcaagatc tacgacccga caggcgtacc cccactcgtt gtcataccag
      661 gaaagggtct tcaccaggtt gcccagggtc atggtatgcc ttgcggcaaa cacggaggac
      721 ctgctgtccc ctgtaacatc aacggagacg atatcatcct cctcgtaagc caggtatcgc
      781 ttcatgggtc cctctgctgc tttaaggaga gctccgttga cctcctctga ggtcacttcc
      841 ctggatagaa ccgttacaag gtcgacaagg gagacgtccg gggtcgggac cctgacggcc
      901 agtccgttca tttttccggc cacctcggga tataccttcc cgatcgccga tgcggcaccg
      961 gtcgtggttg ggatcatgga cagggcagct gcgcggcccc tgtgaaggtt cttgtgggag
     1021 gcatcgaggg tcttctggtc gttggtatag gcatgggtag tggtcatgag acctttctct
     1081 atcacgaaag cctcatggat cacctttacc accggtgcaa ggcagttggt ggtacatgag
     1141 gcattggata ctacgtggtg tctggatggg tcgtagatcc cttcattaac tcccatgact
     1201 atcgttgcgt cctcgttctt ggcaggggcc gtgataagga ccttcctggc gccggcttca
     1261 aggtgcttgg cggctttgga agcatcggtg tacttgcccg atgactcgat aaccaggtct
     1321 attcccatat ccttccatgg gaggtcctca acttcagcct cggcaaggac ctttacggtc
     1381 tttccgttga taaggaggga ctccccctta accgagacat caccgctgaa acgccggtga
     1441 acagagtcat acttgaaaag ataagccctc tgttctgccg tagtccggct gttggttgct
     1501 acgatctcga agagaccatc gttctcgtac tcaaaaaggg ccctgagggc cagacgccct
     1561 atcctgccga acccgttgat cgctacccgt ttcattccca ttccgatcgc ctccggttat
     1621 tttttcataa atacctgttc agcctacgat acttccgcac accccccgag gggcttcaga
     1681 ctgatctgga gggtatccta catgtcctcc cctatcactc tcagaagctc ggagacactc
     1741 ggagaataga gggcgtcccc gtggggcttg tatggcatgc tcccgtcgaa gatctccgcc
     1801 actccaccca gacacccctt tctcaggtgg gagaggaagg gcctgaggaa acaccaggct
     1861 atgtccttcc gatcaggatt gtatctaagg taggctgtga tgaaatgacc cagcagccac
     1921 ggccaggcca ttcccatgaa tctggccttt tctctctggt cgagccgccc ctcgcatcgc
     1981 cccttgtact tctcgtggtg agggtcaagg gtcctgagcc cgaatgtagt atagagttcg
     2041 ttccagcatg tctccaccac cgatctaccc ctttcggggt ccataactgt ggccggaagg
     2101 gagactgcaa ggatctggtt gcacctgatc gattcatccc ttgttccccc gtcgacccag
     2161 tcgtagagat aacccttctc gctccagaag atcttatgga aattccttct gcattcatcg
     2221 gccactgacc cgtaatccct gccatcactt tcacggccga tgatttccga gtacttctcc
     2281 atcaccctca gggagttgta ccagagtgcg ttgacctcta ccagcttccc ccttctctct
     2341 acaatgggtc tgccctcggt ctctccgtcc atccagttgc ttccgggatc gtctgacctc
     2401 agttccagca agcagtcgct gtccattgag ataccgatct cgggaatacc gtttctgtag
     2461 ttctcaagaa cagctgcaag gtcagaccag ttttccctga ccagttccac ctggccactc
     2521 tgatcgcaga acttttccag ggcgtagaag aaccacaacc ccgtatcaca ggccctggtc
     2581 tccagggcac ccgtatgggg gttcacctcc gagggcatta cgtgtccatt cccgacggct
     2641 cgctccagcc ataccctgag gagtctttcg gcgaaccctt tccggcctgt taccagcaag
     2701 aggccgggga cagagacgaa agtctccctt gcactctccc ttaccgaggg gtaacctgag
     2761 aacactttcg gccttttccc gttcatctcg gagagacaat gggatgcgga gtgaagaagg
     2821 tccttcacca tgggcgtct
//
LOCUS       DAOPKQ010000183         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_40497_length_2823_cov_4.953396, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000183 DAOPKQ010000000
VERSION     DAOPKQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40497_length_2823_cov_4.953396"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK146_06315"
     CDS             complement(<1..580)
                     /locus_tag="PK146_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE91888.1"
                     /translation="MSLAMFIQHFFNGLTLGSLYALIAIGYTMVYGILRLINFAHGEI
                     FMMGAYFVFWGVTLFRLPWFIAVIGAIIVTSFAGIMVDRVAYKPLRNAPRISALISSI
                     GVSFFLQNFAIVVFSAIPRQVQRPDWLVKVIVVGKVRILPLTIVVPVLSFVLMMGLLY
                     IVYKTKPGLGMRAISKDIETSRLMGVSVNRIIA"
     gene            complement(682..1920)
                     /locus_tag="PK146_06320"
     CDS             complement(682..1920)
                     /locus_tag="PK146_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE91889.1"
                     /translation="MKKGLLLGLIVVIAVIGFMMMQGQKAPEAPAPEPAAEAPAPEPA
                     EEPAEETAEEPVADAGPIHVGVYLPLTGQNAFGGQLELDGVRMAHEEKPEVLGRKVEL
                     FVVDNKSDKVESANAVKRLIEREKAVAVIGTYGSSLAMAGGEVAESAGIPMVGTSCTN
                     PLVTQGKKYIFRVCFIDPLQGAGAATYANKVIGAQKAALLTDVANDYAVGLAGFFERS
                     FLKLGGEMVSKLNYNSGDQDFTAQLTEIISKEPDILFIPAYFAEGAIIMKQAQELGVE
                     FQIMGGDAMDNPQIVEIGGDAVEGFVHTTFPYDPSMEDMNPVARKFTDNWMAKRSDDP
                     NVNAALGYDSYMILIDAIERAGSADPEAITAALAATKDFEGVTGLTTINETHDAEKPI
                     GIVQIKDGKKQYIATITPEL"
     gene            complement(2131..>2823)
                     /locus_tag="PK146_06325"
     CDS             complement(2131..>2823)
                     /locus_tag="PK146_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPE91890.1"
                     /translation="EEGWHRMTGFPVPLGGYLRISFIDWTGHVAPVLFLCGCNFRCPY
                     CHNPELVTPSGGRLPVPEVLEDIRQRAGFLDGVVISGGEPTIHDSLPEFLSELKSVTG
                     LRIKLDTNGSRPGMLRSVLAGGLVDSISMDVKAPWKMYARITGHGGGPVRESLEILSL
                     SGIPHETRTTFVPDLMSLEDLEVVRDQIPPGSPWIIQPFRGGRTLDPSLEKGTPPDRE
                     KLKINFPDAIIR"
BASE COUNT          607 a          823 c          777 g          616 t
ORIGIN      
        1 gggctatgat cctgttgacg gaaacaccca tcaggcggct tgtctcgatg tccttggata
       61 tggccctcat acccagtccc ggtttggtct tgtaaacgat gtacagcaac cccatcatca
      121 ggacgaagga gaggacaggg accacaatgg tcagcggaag gatccggacc ttccccacta
      181 cgatcacctt taccagccag tcaggcctct ggacctggcg ggggatcgcc gagaacacta
      241 cgatagcgaa gttctgaaga aagaacgaaa cccctatcga actgataagg gccgatatcc
      301 ttggggcgtt ccgaaggggt ttataggcta cacggtcaac cataattcct gcaaaggagg
      361 tgactatgat cgctccgatt actgcgatga accagggcag acggaacagc gttacacccc
      421 agaatacgaa ataggccccc atcatgaata tctcaccgtg agcaaagttg ataagcctca
      481 ggataccgta gaccatggta tatccgattg cgatgagggc atagaggctt cccagggtaa
      541 gtccgttaaa aaaatgctga atgaacattg ccaagctcat atgagctccc ccttgttatc
      601 acgtaaaagg cgggggagga aaaggtaccc tcccccgcac agactccgtt gactctcccg
      661 tggagggcgg ctccgaaaag cctagagttc aggagtgatc gttgctatgt actgtttctt
      721 gccatccttg atctgcacga taccgatggg tttctcggcg tcatgggtct cattgatggt
      781 ggtgaggccc gtcacaccct cgaagtcctt ggtagctgcc agagcagcgg tgatggcctc
      841 ggggtcggcg ctgccggccc gctctatggc atcgataagg atcatgtagg agtcatagcc
      901 gagagccgca ttgacgttcg ggtcatcgga gcgcttggcc atccagttgt cggtgaattt
      961 cctggcgaca gggttcatat cctccatcga aggatcatag gggaatgtgg tgtgtacgaa
     1021 cccttcgact gcatcgccgc cgatctccac tatctggggg ttatccatgg cgtcgccacc
     1081 catgatctgg aactcgacac cgagctcctg ggcctgcttc atgatgatgg ctccctcggc
     1141 gaagtaggcc ggaatgaaca gtatgtcggg ttctttgctg atgatctcgg taagctgggc
     1201 agtgaagtcc tggtcaccgg agttgtagtt gagcttggaa accatttcgc cgcccagttt
     1261 caggaaggat ctctcgaaga agcctgcaag acccacagcg tagtcgttgg caacgtcggt
     1321 cagcagggca gccttctgcg cccctatgac cttgttggca tacgtggcgg cacctgcgcc
     1381 ctgcagggga tcgatgaagc agacgcggaa gatatacttc ttgccctggg taaccagagg
     1441 gttggtgcag gatgtcccga ccatggggat cccggcactc tctgccacct caccgccggc
     1501 catggcaaga gaagatccgt aggtgccgat aacggcgacg gctttctctc tttcgatcag
     1561 gcgtttgacg gcgttagcgg actcgacctt gtcggacttg ttgtccacaa cgaacagctc
     1621 caccttgcgg ccgagaactt cgggcttctc ttcgtgggcc atcctgaccc cgtcgagctc
     1681 aagctgtccg ccaaatgcgt tctgacccgt cagcgggagg tatactccaa catggatcgg
     1741 gccggcatcc gcaacaggct cctctgccgt ttcctccgcc ggttcctcgg cgggctcggg
     1801 agccggagct tcggcagctg gttccggggc cggtgcctcg ggggccttct gcccctgcat
     1861 catcatgaac cctatgaccg cgatgaccac gatcaaacct aataagagac ctttcttcac
     1921 ttatgctcac ctccagatga ttgttcacag cacatcagat tctacacgat cctgccttgt
     1981 tcaggacatc aaggaatacc cgacaccaaa ggggctacca cctctcttcc cacaaaaggc
     2041 cgctcccggg cgaacaggca atgttcccca ccggcagcgg gcatggcctt gacaattacc
     2101 tctgtactac acgcggataa ttatactcta tcaacggatt attgcatccg gaaagttgat
     2161 cttcaacttt tccctgtcgg gaggggtgcc tttctccagc gatgggtcaa gggtcctgcc
     2221 gccccggaag ggctgtatta tccagggtga ccccggcggg atctggtctc tcaccacttc
     2281 gaggtcctcc agactcatca gatcggggac gaaggtggtc ctggtctcat ggggtatccc
     2341 cgacagggac agtatctcca gggattccct gacaggcccg ccgccatgtc cggtgatcct
     2401 ggcgtacatt ttccacgggg ccttcacatc catggatatg gaatctacga gcccacctgc
     2461 caggacggac cttaacatcc cgggcctgct cccgttcgta tccagcttga tcctgagccc
     2521 tgtcacgctt ttgagttccg agaggaactc cgggagcgag tcatggatag ttggttctcc
     2581 accgctgatg accactccgt cgagaaaacc cgccctctgt cttatgtcct ccagtacttc
     2641 agggacaggc agtcttcctc ccgaaggggt caccagttca gggttatggc agtagggaca
     2701 ccggaagttg cacccgcaaa ggaaaaggac cggcgccaca tgaccggtcc agtcaatgaa
     2761 ggagatcctg aggtatcctc ccagcgggac cgggaaacct gtcatccgat gccaaccctc
     2821 ttc
//
LOCUS       DAOPKQ010000184         2803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_40895_length_2803_cov_4.915575, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000184 DAOPKQ010000000
VERSION     DAOPKQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40895_length_2803_cov_4.915575"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PK146_06330"
     CDS             complement(<1..949)
                     /locus_tag="PK146_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPE91891.1"
                     /translation="MPHGIEISGESCRGCVNCIKACPTEAMRVIHGRLKIIAERCIAC
                     GECLRICRHRAISLKESEWASIADDRSMILVTDPALCTQFSWPPDPGILEDILRDIGF
                     DPIFEETALAFDLAALAQARSIEEDDPRELPMISTYCPSVVRLIQIRFPELIPNLSPV
                     ESPLETSIDLWRTRHNRYDVPSALVAPCPARIAMVNRPVGREASSIDHVVSTARVAKE
                     ILSRNYAPSREQDIPKANSRWVNWCVSGGEAMHVRLFENRPLKTLAVSGMRSVVGILQ
                     DIELRRLKGVDFIEARVCDMGCIGGIANAESSFLSRLKVE"
     gene            complement(971..1405)
                     /locus_tag="PK146_06335"
     CDS             complement(971..1405)
                     /locus_tag="PK146_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPE91892.1"
                     /translation="MSRQEPFVIQREVKPLDFLSAGETASDFKETLKSLGIIPEICRK
                     ASIVTYEAEMNAVIHGGGGVIAMELSGDRITIVAKDEGPGIENVSLAMQEGYSTAPDF
                     IREMGFGAGMGLPNIKKYSDELEIETEPGKGMTLRAVIYLRD"
     gene            complement(1411..1767)
                     /locus_tag="PK146_06340"
     CDS             complement(1411..1767)
                     /locus_tag="PK146_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPE91893.1"
                     /translation="MLLGDIMRILEAEPLSQEINFDVDLLSAFAADLMSDVLALARPG
                     TLMITGLTNIQIVRTASMVDMPAVVFVRGKKPPRETIELAEKLGIPFIMTSKTMFETC
                     GVLYQAGIKPIRCLDR"
     gene            2054..2293
                     /locus_tag="PK146_06345"
     CDS             2054..2293
                     /locus_tag="PK146_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPE91894.1"
                     /translation="MATVKVYSTPTCPWCVKLKDYLKSRNVDFENIDVSADRAAAMEM
                     VKKTGQMGVPVALIGDVYIVGFDQARIDEELAKLV"
     gene            2497..>2803
                     /locus_tag="PK146_06350"
     CDS             2497..>2803
                     /locus_tag="PK146_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91895.1"
                     /translation="MEGILNRGSERLLEGARNKDVGFGWFDLPDSPPERLFETVEWLR
                     GFDSVVQIGIGGSALGNRMISSALCHPFYNELTRKERGGPRFYSRQRRSRRDVGHPGH
                     "
BASE COUNT          577 a          757 c          792 g          677 t
ORIGIN      
        1 tctccacctt gagccgactg aggaaggagg actcggcgtt ggctattccc ccgatgcacc
       61 ccatgtcaca aacccttgcc tcaatgaaat cgaccccctt gagccggcgg agctcgatgt
      121 cctgcagaat gccgacgacg ctcctcattc ctgaaacagc cagggtcttg aggggtcgat
      181 tttcgaacag cctgacatgc atggcctctc ctccggaaac acaccagttg acccagcggg
      241 agttcgcttt cgggatgtcc tgttctctgg agggggcgta gttcctggag agtatctcct
      301 tggcgaccct tgcggtggag accacgtggt cgatggagct ggcctcccgg cctacaggcc
      361 tgttcaccat ggctatccgt gccgggcacg gcgccaccag ggcggaaggg acgtcgtatc
      421 tgttgtggcg cgtcctccag aggtcgatac tggtctcaag gggcgattcg acgggggaaa
      481 ggttggggat cagctccggg aaccttatct ggataagcct cactacagag gggcagtagg
      541 tcgatatcat gggcagttcc cggggatcat cctcttctat cgagcgggcc tgggccagcg
      601 ccgcgaggtc gaaggccaga gctgtctcct cgaagatggg atcgaagcct atgtccctca
      661 ggatatcctc caggataccc ggatccggag gccaggagaa ctgggtgcac agtgcggggt
      721 ccgtcaccag tatcatcgac ctgtcatcgg cgatcgaagc ccattcggat tccttcaggg
      781 atattgccct gtgccggcat atcctgaggc attcaccaca ggcaatgcat cgttcggcga
      841 tgatctttag ccggccatgg atcactctca tggcctcggt agggcaggcc ttgatgcaat
      901 tgacacatcc ccggcaggac tcccccgaga tctctatgcc gtgaggcact ggtcgttccc
      961 ccctctcact ttagtccctg agatatatta ctgccctgag ggtcataccc tttccaggct
     1021 cggtctctat ttcgagttca tcggagtatt tcttgatgtt ggggagtccc attccggcac
     1081 cgaaacccat ctccctgatg aaatcggggg cagtggaata accctcctgc atcgccaggc
     1141 taacattctc gataccgggg ccttcgtcct ttgccactat ggtgatccgg tcccctgaca
     1201 gctccattgc tattacgcct ccgcctccat gaatgacggc gttcatctct gcttcatagg
     1261 tcactatcga ggctttcctg cagatctcgg ggattatccc cagggatttc agggtctcct
     1321 tgaagtcgga cgccgtctca ccggcggaga ggaagtccag gggcttgacc tccctttgga
     1381 tcacgaaggg ttcctgtcgg ctcaaagact tcacctgtcg aggcagcgga taggtttgat
     1441 ccccgcctgg tagagcactc cgcaggtctc gaacatggtc ttcgacgtca tgatgaaagg
     1501 gatgccgagt ttttctgcga gctctatggt ctccctgggc ggttttttcc cccggacgaa
     1561 cacaactgcc ggcatgtcca ccatgctggc ggtcctgacg atctggatat tggtcagccc
     1621 cgtgatcatg agggtccccg gtcttgccag ggccaggaca tcgctcatga ggtccgcggc
     1681 gaaggctgat agaaggtcta cgtcaaaatt gatctcctgg ctgagtggtt cggcttcgag
     1741 gatcctcatg atatcgccca ggagcattcc tttccctccc ctgatctgaa aaagtgatta
     1801 caataactat atcatgtttc tttcagaaat gcttcctcta ttagtgaaaa gtttaccatt
     1861 ctttccttta gcttgaccct ttccggggat aggggtcgtt atcttcattt ttccctgttt
     1921 tgcaggagtt tctcttccgc cctgccggag gaggtgataa agtaatggtt gacatacagg
     1981 gacaggagtg taaaatacct aaggggggta tttaggctta ttctcccaaa atgatcatcg
     2041 ggggtggtag gtagtggcta ccgttaaggt ctattcgaca ccgacatgtc catggtgcgt
     2101 caagctcaag gactatctca aatccaggaa cgttgatttc gagaatattg atgtgagtgc
     2161 cgaccgggct gcagcaatgg aaatggtcaa gaagacggga cagatgggtg tccccgtggc
     2221 tttgataggc gatgtctaca tcgtagggtt cgaccaggcc aggatcgacg aggaactggc
     2281 caaactggtg tgacaaaccc ggatttcctg tttgactgta cctgcaggcg gctccccaaa
     2341 aggggccgcc tttttgttct gttaaaatga agtagctcga gatcctgaaa ggtcttcatc
     2401 attcacaggg cggtgatgtt gttgaaagag aagatgctcc gtctgggttt tggagcaggt
     2461 tttccgggtc atggaacgga ggaggttctc ctttccatgg aaggaatcct gaatagggga
     2521 tctgaacgac ttcttgaggg tgccagaaac aaagatgtcg gttttggatg gttcgatctc
     2581 cccgacagcc cccccgaaag actcttcgag accgtggaat ggctcagggg tttcgattcc
     2641 gtcgtccaga tcgggatagg cggatctgcc ctgggtaaca ggatgatatc gtcggctctt
     2701 tgccacccct tctacaacga actgaccagg aaagagagag ggggtccgcg cttctacagc
     2761 agacaacgcc gatcccgaag ggacgtcggc catcctggac atg
//
LOCUS       DAOPKQ010000185         2801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_40938_length_2801_cov_3.142025, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000185 DAOPKQ010000000
VERSION     DAOPKQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40938_length_2801_cov_3.142025"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..912)
                     /locus_tag="PK146_06355"
                     /pseudo
     CDS             complement(223..912)
                     /locus_tag="PK146_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
     gene            complement(909..1280)
                     /locus_tag="PK146_06360"
     CDS             complement(909..1280)
                     /locus_tag="PK146_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPE91896.1"
                     /translation="MARHLDLGMAGESLACRELSRLGYRIIETNVRMKMGELDIVAEE
                     GGEMVVVEVRTRTLGRMLPPEATVGPSKIRKLVRTGTAWMDSRGWEGPWRIDIAAITV
                     DGRGKPSLEIFKDITVGLIQP"
     gene            complement(1345..1920)
                     /locus_tag="PK146_06365"
     CDS             complement(1345..1920)
                     /locus_tag="PK146_06365"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPE91897.1"
                     /translation="MIVAGVDEAGRGPLAGPVVAAAVVLDRHQTKSLLSMGLNDSKKI
                     SPSRRERIFGGMNSMGVAWRAQAASHHRIDRDNILAATLWAMGRCVCALPVVPDLVVV
                     DGSVAIPMPGIRQKTLPGGDGIVPAIAAASVVAKVLRDRVMVALDALFPEYCFKRNKG
                     YPSREHVERLRACGPCPVHRRSFNVRGWSAS"
     gene            complement(1917..2756)
                     /locus_tag="PK146_06370"
     CDS             complement(1917..2756)
                     /locus_tag="PK146_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPE91898.1"
                     /translation="MTRTVWYPGHMARGMRKLRELSERLDLFIEVRDARAPLLTGSPF
                     SEQAARLRPVWIVLSKKDLADQEATARWLSFFRDKGLRAWALDLRKGGVEAVTREAGK
                     LRPPHRELRLAVIGIPNVGKSLFLNSIVGKSASPVGGIPGITRGVSWYRGKGCLVVDS
                     PGIVDPRSHREVHRRLAWLGSTKTDVIGGYEDLAEGLVDFLGRSPMLERALGTWEISA
                     EGLDRAEILQRIGARLGCLVKGGMVNRDLAGRRFIEAFSTGKLGRFTLEDPEERPFWE
                     DLS"
BASE COUNT          539 a          943 c          753 g          566 t
ORIGIN      
        1 cagccatatg atcattatag tcggctctgg aatgggcgaa aatgatatcg ggccggaaag
       61 acctgatatg cccgataacc tcgcggtaag aggcgttgtt tacacccaaa ctccaatcca
      121 ggcctttcag atttatatat tcctgaatgc ccagaacccg gcaaactgct tccgtttcct
      181 catggcgtat ccgaactatc gattttttat cctccggttt cgaatatcct tcagcgccct
      241 cgcgagaagg gtcttccccg atcccggcga gcccgtcaga agaacgttgt ggtgacccgc
      301 cgcagctatt tccagggctc tcttggcagc cgtctgcccc ttgatgtccg caaaatccgg
      361 atccggttcg tggaactcgt ctccgggtgg cgacgggacg atccgctcga gttgtccccc
      421 ttcacgaagg aaagagaaaa gatcccggag atgatccacc ctgtaggcct cgacgccctt
      481 cacgagggag acctccctgg cgttgccgcc gggaacgaag agtggcaggc ccctctccct
      541 tgccagaagc gcagcgggga cggctccccg gacccttcgg accctgccgt caagagcaag
      601 ttcacccata aagaccgaag gtcgggagac cctcacctcc cccatggcgt gggcaatgcc
      661 cacggcgatg gcaaggtcca gaagggcccc ctccttgggg ctgtctgcgg gcgcaagatt
      721 taccgcgacc ctccccctga cggatacccc cagcgatctc agagccgctc ggaccctctc
      781 cctggcctcc cttacagacg tatcgggaag cccgacgatg gagatcacga aaagtccgcc
      841 cgttatctcc acctccacct ccacccttat ggcctctatc ccctgaaggg tcacgcccag
      901 tacggcgttc atggctgaat gagccccacg gtgatgtctt tgaatatctc cagggagggt
      961 ttgccccttc cgtcaacggt gatggcagcg atgtcgatcc tccaggggcc ctcccacccc
     1021 cgggaatcca tccaggccgt cccggtcctg acaagtttcc ttatctttga gggcccgacg
     1081 gtggcctccg gcgggagcat cctcccgagg gtccttgtcc tgacctccac cacgaccatc
     1141 tcgccaccct cttcggcgac tatgtcgagt tcccccatct tcatccggac gtttgtctcg
     1201 atgatcctgt acccgagacg cgacagctcc ctgcaagcca ggctttcgcc cgccatgccg
     1261 agatcaaggt gtctggccat accggctcct ttcgaataac acgggattaa accgccgatg
     1321 atgcgagggc gggccctgcg gacgtcagga agcggaccag cccctgacgt tgaaacttct
     1381 cctgtgcacg gggcagggac cacaggcccg caacctttcc acatgttcac gggaaggata
     1441 ccccttgttc cttttgaaac agtattcggg aaaaagagcg tccagcgcca ccatgaccct
     1501 gtcccgcagg acctttgcca ccaccgatgc ggcggcgatg gcgggaacta tcccgtcacc
     1561 tcccggcagt gtcttttgcc ggatgcctgg catgggtatc gccacgctcc cgtcaactac
     1621 cacgagatcg ggtaccaccg gcagggcgca cacacacctg cccatcgccc agagtgtcgc
     1681 cgcaaggatg ttgtcgcggt ctatgcggtg atgagaggcg gcctgcgctc tccatgcgac
     1741 acccatggag ttcatcccgc caaagatcct ttccctccgg gaaggagata ttttctttga
     1801 gtcattcagc cccatcgaaa ggagcgactt cgtctgatgc cggtcgagaa ctaccgcggc
     1861 ggccacgaca gggccggcca ggggccccct gcccgcttcg tcaacccccg cgactatcaa
     1921 gagagatcct cccagaaggg cctctcttcg ggatcctcca gggtgaatct gccaagtttg
     1981 ccagtcgaga aggcctctat gaatcttctc cccgcaaggt cccggttgac cattccgccc
     2041 ttgaccaggc accccagtcg cgcgcctatg cgctggagta tttcggcccg gtcaagacct
     2101 tcggcggata tttcccaggt acccagggct ctctccagca tgggcgaacg gccgagaaaa
     2161 tccacgaggc cctcggccag atcctcgtac cccccgataa cgtccgtttt ggtgcttccc
     2221 agccatgcaa gccgccggtg aacctcgcgg tgcgagcggg ggtccactat cccgggcgaa
     2281 tcaacgacca ggcatccctt gcccctgtac caggagactc cccttgtgat cccgggtatg
     2341 cccccgacag gtgatgcgct cttacccacg atggagttta aaaacaaaga tttgcccacg
     2401 ttcgggatgc ctattacggc gagcctgagt tccctgtggg gaggacgcag tttgccggcc
     2461 tccctggtta ccgcctctac ccctcccttg cgaaggtcca gggcccatgc ccttaaaccc
     2521 ttgtcccgga aaaaggaaag ccaccttgcg gtggcctcct gatctgcaag gtcctttttt
     2581 gacaatacga tccataccgg gcgcagtctt gcggcctgtt cgctgaaggg cgaccccgta
     2641 agaagggggg ccctcgcgtc gcggacctcg atgaaaaggt ccagtctttc tgatagttcg
     2701 cggagttttc tcatcccccg ggccatgtgg cccgggtacc agacggttct ggtcattcct
     2761 cgtaaaggcc gcctatcctc gagagcggcc agtatcgcag a
//
LOCUS       DAOPKQ010000186         2789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_41178_length_2789_cov_3.765179, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000186 DAOPKQ010000000
VERSION     DAOPKQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41178_length_2789_cov_3.765179"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PK146_06375"
     CDS             <1..1178
                     /locus_tag="PK146_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HPE91899.1"
                     /translation="LSSPFSKPVMMKRSDRQRSGLPPAISLYVHVPFCRSKCPYCAFY
                     SFPPAVGDIPSFLQALEKEIALLGREGDLPRIRTAYIGGGTPSLLSVPDWRRLIRILE
                     RAFTFIPGAEVTVEANPASLTGGHLSLWKDWRVTRVSLGVQSLQDKDLLAAGRAHDRR
                     LALDSMTRISGTDLSLSVDLLFGLPGQHFRGWGETLREILSEGVDHVSIYQLTLEEGT
                     PWGERPPNDLAEGYPFYRWAQYYLEKKGLLQYEIASFARPGRWCRHNVSYWRGGEFIG
                     LGPSAWGYRKYQRTRNIPDLPEYCSMVARGKSPVCYSERLEGDRLASESAILALRTRW
                     GIRLQDLRRDWGDDLPERMAAALKELPRDLFRRSPGRIALSRRGMRVGNSIWERLVP"
     gene            complement(1193..1795)
                     /gene="yedF"
                     /locus_tag="PK146_06380"
     CDS             complement(1193..1795)
                     /gene="yedF"
                     /locus_tag="PK146_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPE91900.1"
                     /translation="MHKVDARNRPCPKPVIMTREALKSHGLPLEIVVDSGIPLLNVRR
                     FLESQNIRVSFSEAGGYATLVCTEGDQAEGALQAGTAEKRDERPEDVAILVCSEFLGH
                     SDRLLGDVLIKSFLGTLVEREQPPALIALMNEGVKLALPGSSASESLQALQARGTEIL
                     VCGTCSSHFGITDSISVGIISNMFDITGSLLRHQKSIVLG"
     gene            1941..>2789
                     /gene="selA"
                     /locus_tag="PK146_06385"
     CDS             1941..>2789
                     /gene="selA"
                     /locus_tag="PK146_06385"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPE91901.1"
                     /translation="MRDEIRSLLRDLPSMDELLQKRWTGRFQEILGREAVRSTFADVI
                     GEVRRDLIKGRIKSIDPGHIESEARSRLEKYSRKSLRRVINATGVVVHTNLGRSCLGA
                     GVLENVMQASGCYSNLEYDLEEGLRGHRNSHVEWLLTSITGADSALVVNNNAGAVLLC
                     LAALGRDREVIVSRGELVEIGGSFRIPDIMEFSGALMKEVGTTNRTHLSDYLNALSPA
                     TGMILKVHPSNFRVVGFQAEAAREDLASLAREKDLIFMEDLGSGVVVDLGAWGLQGEP
                     TVRQCIE"
BASE COUNT          606 a          715 c          865 g          603 t
ORIGIN      
        1 agctttcctc gcctttctca aagccggtga tgatgaagag gagtgacagg cagagatccg
       61 gcttgcctcc cgccatatcc ctgtacgtgc atgtcccttt ctgccggagc aaatgcccct
      121 actgtgcctt ttacagtttt ccgcccgccg ttggagatat accctcattc cttcaggctc
      181 tcgagaaaga gatagcgctt cttgggagag agggtgatct gccccggatc aggacagcat
      241 atattggggg aggcacccca tcgcttctct ccgttcccga ctggaggagg ttgatcagga
      301 tcctggagag agccttcaca tttatacccg gggcggaggt gacggtggag gccaaccctg
      361 cctcattgac gggggggcat ctctccctct ggaaggactg gagggttacc agggtaagcc
      421 ttggggtcca gagcctccag gacaaggatc ttcttgccgc aggaagggct catgaccgaa
      481 ggttggccct tgattccatg acaaggattt caggaacgga tctttcacta tcagtggacc
      541 ttctcttcgg gctgccagga cagcatttca ggggctgggg tgaaacactg agggagatcc
      601 tttccgaggg agtggatcac gtttccatct accagctcac ccttgaagag ggtacgccct
      661 ggggggaacg ccctccgaac gaccttgcag aagggtaccc cttttaccgc tgggcccagt
      721 actaccttga aaaaaagggt cttctccagt acgagatagc cagtttcgca aggcccggga
      781 ggtggtgccg gcacaacgta tcctactgga ggggggggga gttcatcggt ctggggccgt
      841 cggcctgggg gtacaggaaa taccagagga caagaaatat tcccgacctt cccgaatact
      901 gttcaatggt ggcacgaggc aagagtcccg tctgttattc cgagaggctg gagggggatc
      961 ggcttgcctc cgaatcggcc atcctggccc tgaggacccg gtggggcata aggctacagg
     1021 atctccggcg agactggggt gacgacttgc cggaaagaat ggcagctgcc cttaaagaac
     1081 tgccccggga ccttttcagg aggtcgccgg ggaggatcgc cctgagcaga aggggtatga
     1141 gggtggggaa ctccatctgg gagagacttg tgccctgatg gcaggggtgt tcctaaccca
     1201 gaacgatact cttctgatgg cgcaaaaggg atccggtgat gtcgaacatg ttggagatga
     1261 tccccactga aatggaatcg gtgatcccga aatggctgga gcaggtcccg catacaagta
     1321 tttccgttcc cctggcctga agggcctgaa ggctttcgga ggccgatgag ccgggaaggg
     1381 ccagtttgac accttcgttc ataagggcga tcagcgccgg aggctgctct ctctccacca
     1441 gggtccccag gaagcttttt ataaggacat cccccagtag acgatcactg tggcccagaa
     1501 actcggaaca gacaagaatg gctacgtcct ccggtctctc gtcgcgtttt tcggcagttc
     1561 cggcttgaag agcgccttca gcctgatcac cttctgtgca gaccagggtg gcgtagccgc
     1621 cggcctcgga gaaggagacc cttatgtttt ggctttcgag gaaacgcctt acgttgagaa
     1681 ggggtatccc gctgtctacg actatttcaa ggggcagccc atggctcttc agcgcctctc
     1741 tggtcattat caccggtttg gggcagggcc tgttccttgc gtccaccttg tgcatgttct
     1801 cctacctccc cttatacttg tgaggatgac gagagcaata ataccatcga cggaagctct
     1861 ggtatcattc tgttgggtca agggataaag ccggggacgg aaccgtagag gtccgcccct
     1921 cttcagggga tggtgcgaag atgcgtgacg agataagatc gttgttaagg gacctgcctt
     1981 cgatggatga acttctgcag aagagatgga caggccggtt ccaggaaatc ctgggacgtg
     2041 aagccgtcag atcgaccttt gcggatgtga taggtgaagt acgaagggac ctgataaagg
     2101 gtcgcataaa atccatagac ccaggtcata tcgaatcaga ggccagatca aggctggaga
     2161 agtactcaag aaagagtctc aggagggtga tcaatgccac aggggtggtg gtccacacca
     2221 acctgggaag gtcatgcctg ggagctggag tgcttgaaaa cgtcatgcaa gcctcggggt
     2281 gttacagcaa tctggaatac gatctggaag aggggttgag aggccacagg aactcccacg
     2341 ttgaatggct tctcacaagc atcaccgggg ctgattccgc gctggtggtg aacaataacg
     2401 ccggtgcggt tctgctctgt ttggctgccc ttggtcgaga cagggaggtt attgtctcac
     2461 ggggagagct ggtggagata gggggttctt tccggatccc tgacatcatg gaattctcag
     2521 gagcccttat gaaagaggtc ggtaccacca acaggaccca cctctctgac tacctgaacg
     2581 ccctgtctcc agccacgggc atgatactca aggttcaccc ctccaacttc agggtggtgg
     2641 gtttccaggc cgaggcggcc agggaagatc tggcctccct ggcaagggag aaggatctga
     2701 tcttcatgga ggaccttggc agcggagtgg ttgttgacct tggtgcctgg gggctgcagg
     2761 gagagcctac ggtcagacag tgtattgag
//
LOCUS       DAOPKQ010000187         2760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_41774_length_2760_cov_5.023290, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000187 DAOPKQ010000000
VERSION     DAOPKQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41774_length_2760_cov_5.023290"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PK146_06390"
     CDS             complement(<1..239)
                     /locus_tag="PK146_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HPE91902.1"
                     /translation="MPPVFFEIPLNADLETVKRQAGSRKFDARLVRGVSRRCRYGFPQ
                     VTVCDPLRKGHPFPTVFWLSCPCLSRKVDRIESAGG"
     gene            complement(275..697)
                     /locus_tag="PK146_06395"
     CDS             complement(275..697)
                     /locus_tag="PK146_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPE91903.1"
                     /translation="MVQEKGSQTTEVKVGDIVKGTVEQILPYGAFVRLSTGQKAMIHI
                     SELAYEYVKKVEDILQLQQEVEAKVIKIDERGRIDLSLKQLQARVERPRQPRGAPSSA
                     GDDFEKKLASFLKASDQKLSDLNRKNKEGSGGKPRGKK"
     gene            complement(842..1675)
                     /gene="codY"
                     /locus_tag="PK146_06400"
     CDS             complement(842..1675)
                     /gene="codY"
                     /locus_tag="PK146_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HPE91904.1"
                     /translation="MNTPKELIESRAKIADPSAMQDLLEKTRAVNRVLQSRRDPGTPD
                     YQRLARLLCELSAANVYMIDREGGILGYSWISEYNCPIMSELLEKGAMPEGYTEKVNQ
                     YHESELNHTDHGLCAYSDEPCTYSNKHVVYVPIHGAGERLGTLILARFGHPFDNRDLV
                     LGEYLATVVGLEILHSRARSIEDRARERLIVQMAMRALSYSEVESVRHMIRELGGSEG
                     IVVASRVADRVGVTRSVIVNALRKLESAGIIESRSLGMKGTFIKVLSPLFLEDLGVTG
                     H"
     gene            complement(1728..>2760)
                     /gene="hslU"
                     /locus_tag="PK146_06405"
     CDS             complement(1728..>2760)
                     /gene="hslU"
                     /locus_tag="PK146_06405"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPE91905.1"
                     /translation="VRTRERILDALIPPRKVQEKVSDFMHIFSGQPQKEERIPTEEEE
                     RAERQRLSTRERLGRMLDEGKLEGREIEIEVSESAQFGIPVMGGPGMEEMGMNIGEML
                     SGIFPKKKKRRTVTVETARSILEAEEAEKLVDHEAAAQEGLKKAQEEGIVFIDELDKV
                     ASAGGGHSGPDVSREGVQRDLLPIVEGCTVNTKYGPVRTDHILFIAAGAFHKMKPSDL
                     VPELQGRLPLRVELNPLAEGDLARILVEPEHSLVDQYKALLSTEQVTLSFSETALREI
                     ASLAARMNRETEDIGARRLHTMMENLLEEIQFEAPDLSGQNVEIDGELVRERLERLVA
                     DTDVRKYLL"
BASE COUNT          501 a          883 c          716 g          660 t
ORIGIN      
        1 cctccggccg actcgatcct gtcgactttc cttgagaggc atgggcagct cagccagaag
       61 accgtgggga aggggtgccc ctttctcaaa ggatcgcata ccgtcacctg tgggaagccg
      121 tacctgcacc ttcgggaaac gccgcgtacc aggcgggcat caaatttcct tgaaccggcc
      181 tgcctcttca ccgtttcgag atcggcgtta aggggtatct caaaaaaaac agggggcaac
      241 aagccccctg cagcaggcag atcctgctcc tgtcctattt ctttccccga ggttttcccc
      301 cggagccttc cttgttcttc ctgttcagat cggataattt ctgatcgctc gccttcagga
      361 atgaagccag tttcttctca aaatcgtctc ccgccgacga tggcgcgcct cgcggctgcc
      421 tcggtctctc tacgcgggcc tggagctgtt tcagggagag gtcgatacgg cctctttcat
      481 cgatcttgat gaccttggcc tctacctcct gctgcagctg caggatatcc tctaccttct
      541 tgacatactc gtaggcaagc tccgagatat ggatcattgc cttctgcccc gtcgaaagcc
      601 tgacgaaggc cccgtatggc aggatctgtt ccacagtacc ctttacgatg tctccgacct
      661 taacctccgt cgtttgggag cccttttcct ggaccatcaa ccagttttcc cctccgtata
      721 gatttgagag atgatctata aaaacaccgg cccgtacaga tccggttcac gccgacaagt
      781 atatcattat gtcaaggaca gggtacaact cctgtcatct tccgtaacgg cgtaacaaag
      841 atcagtgtcc ggtaacccca aggtcctcca ggaatagggg gctcaggacc tttatgaaag
      901 tgcccttcat gccaaggctc cggctttcga ttatacccgc gctctcgagc tttctcaggg
      961 cgttcacgat gacgctccgg gtcaccccca ccctgtctgc aacacggctg gctactacga
     1021 tcccttccga tcctcccagc tccctgatca tgtgcctgac ggactccacc tccgagtagg
     1081 acagggccct catggccatc tggacgatca ggcgctccct ggccctgtcc tctatgctcc
     1141 tggccctgga atggagtatc tccagaccta ccaccgtggc cagatattca ccgagcacaa
     1201 ggtccctgtt atcgaaggga tgaccgaacc gggccaggat aagtgtaccc aggcgctccc
     1261 ccgcgccgtg gataggaaca tatacgacgt gcttgttgga ataggtacag ggctcgtcgg
     1321 agtaggcgca gagtccgtgg tcggtgtggt tcagctccga ttcgtggtac tggttcacct
     1381 tttcggtgta cccttcaggc atggccccct tctcaagtag ctcagacatg atggggcagt
     1441 tgtactcgct gatccaggag tagcccagga tcccgccctc ccggtcgatc atgtagacgt
     1501 tggccgccga cagttcgcag agaagcctgg cgagcctctg gtagtccggt gtacccggat
     1561 ccctgcgact ctgcaggacc ctgttgacgg cacgggtctt ctcaaggagg tcctgcattg
     1621 ccgaaggatc ggctatcttg gcccttgatt cgatcagttc tttcggtgtg ttcatcgtca
     1681 cagagatccc tcccgatgtt ctcaggcgct gatcgggcag cgcccaacta taacaggtat
     1741 ttccttacat ccgtatcggc taccagcctc tcaagccgtt cccttacaag ttcgccatcg
     1801 atctccacgt tctgcccgga gaggtccggg gcctcgaact ggatctcctc caggagattc
     1861 tccatcattg tgtgaagccg gcgggccccg atgtcctcgg tctcgcggtt catcctggcg
     1921 gccagcgagg cgatctccct cagggctgtc tccgaaaagg aaagggttac ctgctccgtc
     1981 gaaagcagcg ccttgtactg gtccaccagg ctgtgctccg gttccacgag tatccttgcc
     2041 aggtccccct cggcaagagg gttgagctcg actctgagag gaagccttcc ctggagttca
     2101 gggacgagat ctgacggttt catcttgtgg aaggcaccgg cagctatgaa aaggatgtgg
     2161 tcggtcctca ccggtccgta cttcgtgttg accgtacacc cctcgacgat gggaagaagg
     2221 tctctctgga ccccctcgcg gctcacgtcg ggtcctgagt gaccgccacc tgccgaagcg
     2281 accttgtcga gttcgtcaat gaagactata ccctcctcct gggccttctt cagaccctcc
     2341 tgggctgcgg cttcatgatc tacgagcttc tccgcctcct cggcctccag aatgctcctg
     2401 gccgtttcga cggtaacggt ccgcctcttt ttcttcttcg ggaatatgcc gctaagcatc
     2461 tccccgatgt tcatgcccat ctcttccata ccgggcccgc ccatgacggg gatgccgaac
     2521 tgggcgctct cgctgacctc gatctctatc tcgcgaccct ctagcttgcc ctcgtcgagc
     2581 atacgcccca acctctccct ggtggagaga cgctgcctct cggctctctc ctcctcttcg
     2641 gtagggatcc tctcctcttt ctggggctgc ccggagaaga tgtgcatgaa gtcggagacc
     2701 ttttcctgga cctttctcgg cgggataagg gcgtcaagta tcctctccct cgtcctgaca
//
LOCUS       DAOPKQ010000188         2743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_42178_length_2743_cov_5.042411, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000188 DAOPKQ010000000
VERSION     DAOPKQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42178_length_2743_cov_5.042411"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="PK146_06410"
     CDS             complement(<1..90)
                     /locus_tag="PK146_06410"
                     /inference="COORDINATES: protein motif:HMM:NF015302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPE91906.1"
                     /translation="MAGGAFFSLASVIGIIRFPDIYTRIHAGTK"
     gene            complement(126..383)
                     /locus_tag="PK146_06415"
     CDS             complement(126..383)
                     /locus_tag="PK146_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPE91907.1"
                     /translation="MITAMIWALMISAALGFWRVIVGPTVPDRIVAADALSIIMTTTL
                     ALLGLVFNNPVFLDIAIAFAVLAFADVLIMAKFFEHGELHL"
     gene            complement(380..901)
                     /locus_tag="PK146_06420"
     CDS             complement(380..901)
                     /locus_tag="PK146_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPE91908.1"
                     /translation="MDPSRKNRFVLLVALVILWVMLTGSFEPASLGAGIAVSALIVQL
                     TWRPLLLGTRHVGAPSARPLDIKPLEILKIFPRFMFDILKASAEVAIIALKPRIDIRP
                     GIVRVDSHLKNKTALVFLANYITLTPGTLTVDIDTARHHVFIHALDLGTLDAPDIRNE
                     VAMLEKRLGRVLE"
     gene            1061..1717
                     /locus_tag="PK146_06425"
     CDS             1061..1717
                     /locus_tag="PK146_06425"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HPE91909.1"
                     /translation="MPQDKRIREGTLSKGRIEALADGIFAVAMTILVLDIRAPSYSVR
                     LDRKAFDAFVEGLGDPLMNYLISFLLLSLFWIWHHRQSTRIRRTDPVHLAINLLFLAA
                     VCLVPFSTSTLSRFMDILEADLIFHLNIFVLGSLMMANWWYATSGNRLVDSDIDPERV
                     RSGKRRSMVAPVLAVLGMVASFFIMGNSTIIYILTPFLTWMAGKGLSRPRQEGEGNWD
                     "
     gene            1747..2661
                     /locus_tag="PK146_06430"
     CDS             1747..2661
                     /locus_tag="PK146_06430"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HPE91910.1"
                     /translation="MYGCGALGSRLAVQFHEVGMPVRCLSRPGIHVERIRSRGLVYGG
                     SGQKTRTVCLEIHDDPSSCPPADLVIVLVKSWQNPEVAANLKGLLSPDGWVLTLQNGL
                     GNVEALQEHVPEGKLLAGSISYGAFRPEPGVVYCGGDGFIRFAPVTPGVDAKDVLGLF
                     RAAGFNADLEDEPWRAIWAKVIVNAGVNPVAALTRSSNAQILGSPFARKVMESLCREA
                     VSVADAEGIPFDPDEQWSALLEILELTSGNRPSMLQDILSGNRTEIEAISGEIMRRGI
                     RAGLEVPVTETVCYLVKALEVSVSRGVS"
BASE COUNT          610 a          722 c          833 g          578 t
ORIGIN      
        1 ctttgtgccc gcatggatac gggtatagat atccgggaac cttatgatgc ctataacgga
       61 ggccagagag aagaacgcgc cccctgccat cagcacatag gcagcggact cgcgaaataa
      121 cgtgctcata ggtgcagctc tccgtgttca aagaacttgg ccatgatcag gacatccgca
      181 aaggccagta ctgcgaaggc tatggcgata tcgaggaaga cagggttgtt gaaaaccagc
      241 cccaggaggg cgagggtagt ggtcattata attgaaaggg catcggctgc cactatcctg
      301 tcagggaccg tagggcccac tatgacccgc cagaaaccca gggcggctga gatcatcagt
      361 gcccagatca tcgccgttat cattcaagga ccctccctag acgcttctcg agcatggcta
      421 cctcgttgcg gatatccggg gcgtcaagtg ttccaagatc gagggcgtgg atgaacacgt
      481 ggtggcgggc cgtgtcgata tctacggtga gtgttccagg cgtgagggtg atatagtttg
      541 ccaggaagac cagggccgtc ttgttcttga ggtgtgaatc cacccttact atgccaggcc
      601 tgatatctat tcgcggcttg agcgcgatga tagccacctc tgccgaggcc ttgagaatgt
      661 cgaacatgaa gcggggaaat attttcaata tctccagggg tttgatgtca agggggcggg
      721 ctgagggggc acccacgtga cgtgtgccca gaagcagcgg cctccaggtc agctggacaa
      781 tgagagcgct tactgctatc ccggcaccca gtgaagccgg ctcgaaagac cccgtgagca
      841 tgacccacag gatgacaagg gcgacaagga ggacaaaacg gttcttcctg gagggatcca
      901 aggcggctcg agcccccttt cctcgggtga agactgatgg accggagaca acaagaatta
      961 tattggaaaa ggacctttgt tgcctaattt atgtatgcag aagcctgttc tcgcagatat
     1021 aatgtagagg tcgttaatat ttcaagaggg gggatgaagg ttgccgcagg acaaaaggat
     1081 ccgtgaaggc actctctcga agggtcgtat cgaggccctg gcggacggca tcttcgccgt
     1141 ggcgatgacc atccttgtcc tggacataag ggccccctca tattccgtca ggctggacag
     1201 gaaggccttc gatgcctttg tggaggggct tggggatcct ctcatgaact acctcatcag
     1261 tttccttctc ctgtctcttt tctggatctg gcatcacagg cagagcaccc ggataagaag
     1321 gaccgatccc gtccacctgg cgatcaacct ccttttcctt gcggccgtat gtctggtccc
     1381 cttctccacg tcgacattaa gcaggttcat ggacattctg gaggcagacc tgatattcca
     1441 cctgaacatc tttgtacttg ggtccctgat gatggccaac tggtggtacg ccacatcagg
     1501 caacaggctg gtagacagcg atatagaccc cgagagggtc aggtcaggaa agcggaggag
     1561 catggtcgcg ccggtgcttg ccgttttggg catggtggca tcctttttca taatgggaaa
     1621 cagcacgata atatatatcc tgacgccctt ccttacctgg atggccggca aaggtctctc
     1681 ccggcccaga caggaggggg agggtaattg ggactagaca ggaaagggtg atctgtcacg
     1741 ataaccatct atggatgcgg agccctgggg agtcgcctgg cagtccagtt ccacgaggtt
     1801 ggcatgcctg tccgttgcct ttctcgcccg ggaattcatg ttgaaaggat aaggtcccgc
     1861 ggactggttt acggaggatc cggccaaaag accaggacgg tgtgcctgga gatccacgac
     1921 gatccttcca gctgtccccc tgccgacctt gtgatagtcc ttgtcaagtc ctggcagaac
     1981 ccggaggtgg cagccaacct taagggcctt ctctcccccg atggatgggt cctgaccctg
     2041 cagaacggac tcgggaacgt ggaagcactg caggagcacg tccccgaagg aaaacttctt
     2101 gcgggatcta tcagttacgg agccttcagg ccggagccgg gagtggtcta ctgcggaggg
     2161 gacgggttta tccgtttcgc cccggtaaca ccaggggttg atgccaaaga tgttctcggg
     2221 cttttccggg ccgcaggctt caacgccgat cttgaagacg aaccctggag agcgatctgg
     2281 gcaaaggtca tcgtcaacgc cggtgttaat cccgtggcgg ccctgacccg ctcatccaac
     2341 gcccagatcc tgggttcacc ctttgccagg aaggttatgg agtccctctg ccgtgaggca
     2401 gtgtcagtgg ctgatgccga aggcataccg tttgaccccg acgagcagtg gtcggctctg
     2461 ctggagatcc ttgaactgac atcgggaaac cggccatcga tgcttcagga tatcctgtcg
     2521 ggcaacagga ccgagataga ggccataagc ggcgagatca tgagaagagg catccgggca
     2581 ggccttgaag tgccagttac ggagacggtc tgctatcttg tgaaggccct ggaggttagc
     2641 gtgagccgcg gggtttcctg agaaacctga tcaccggcac cgagacgcca gcggctgaaa
     2701 gggcgtaggc cagtgccctc atggatgtag agtcgaagtc ggt
//
LOCUS       DAOPKQ010000189         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_42379_length_2734_cov_4.165360, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000189 DAOPKQ010000000
VERSION     DAOPKQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42379_length_2734_cov_4.165360"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK146_06435"
     CDS             <1..194
                     /locus_tag="PK146_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPE91911.1"
                     /translation="SGRVVSIVPRGMAFEVVFQVEDLRLTGRMLSSALVREDVHPGGE
                     CRVGFCPEAIHVFKGDNPI"
     gene            211..1170
                     /gene="wtpA"
                     /locus_tag="PK146_06440"
     CDS             211..1170
                     /gene="wtpA"
                     /locus_tag="PK146_06440"
                     /inference="COORDINATES: protein motif:HMM:NF003196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HPE91912.1"
                     /translation="MGKFKRSVLLVTVLSLLVVATATAALAGEKVIVFHAGSLSAPMA
                     EIEGLYEKANPDVDVQREAGGSAALARKITDLGGACDVFLSADYMVIEKLLRPAVADW
                     NVLFASNAIVLMYGPKSKYAGEINKDNWTEVLMRTDVRWGHSEPDADPCGYRSLMVLQ
                     LAEEFYGDKDLYARAMEHPERAVRPKAIDLVAMVESGAMDYAFEYKSVAVQHGLNYLD
                     LPDEINLKDPGFAEKYAAASVELAGKEPGQKMTVKGEPIVYGLVIPKDAPNMEGALKL
                     VEFILAQDGGLKVFEEMGQDIVGPQTITPGEKVPAGVEALLVE"
     gene            complement(1251..1460)
                     /locus_tag="PK146_06445"
     CDS             complement(1251..1460)
                     /locus_tag="PK146_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPE91913.1"
                     /translation="MKLSARNALKGKVKEVKAGAVNTEVIVELKGGEEIVSMISKDSA
                     DKLGLKPGKEVYAVIKASNVMIGVD"
     gene            complement(1565..>2734)
                     /locus_tag="PK146_06450"
     CDS             complement(1565..>2734)
                     /locus_tag="PK146_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HPE91914.1"
                     /translation="TLYSRVFLLFQVIACGENIMAKIGSFEFSLRELGGSLGDLGTLL
                     PLAVGYITVCGIDPAGMLVFMGLANILTGIIYRLPMPIEPMKVLAVMAIAQAWTPDKV
                     FMSAFAMGVTWLILGFSGAITWIASRTPQAVIRGIQLSLGLLLAMQGFRMIDGAWILA
                     GLSILLILVLRDNRFAPASLVLVLGGVLLMAFRGDLSQIQSVTFTLPPLHPLRLELFW
                     PSMRDGGFSQIPLTATNAVIATSALISRYWPDRSVSARHLSINMGVMNLLGPFIGGIP
                     LCHGAGGLAGQYYYGARTGGANVIEGFLEILLGIFFAGHIATIFRSFPMAIVGAMMIM
                     VGVEMAKFTRSVRGPGEWSVVAVTVGFSLYLNMAAGFAAGLAAAWIIAGHGKTGN"
BASE COUNT          616 a          747 c          823 g          548 t
ORIGIN      
        1 tctccggccg ggtggtctcc atagttccaa gaggtatggc cttcgaagtg gtcttccagg
       61 tggaagatct tcgtctcacc gggaggatgc tctcctcggc acttgtcagg gaggacgttc
      121 accccggagg agagtgcagg gtaggattct gccctgaagc gatacatgtt ttcaagggag
      181 ataatccaat ttaacggagg tgcaagggta atgggtaagt tcaaaagaag tgttttactg
      241 gtaacggtcc tgtccctgct agtggtcgcg acggcgacag cggctctggc aggagagaag
      301 gtgatcgtct tccacgcggg aagcctttcg gcccccatgg cagagatcga ggggctttat
      361 gaaaaggcaa accccgatgt ggatgtgcag cgtgaggccg gcggcagcgc cgcccttgcc
      421 aggaagataa ccgacctggg gggtgcctgc gacgttttcc tgtcagctga ctacatggtg
      481 atcgaaaaac tgcttcgtcc ggccgtagcg gactggaacg tcctgttcgc aagcaacgcg
      541 atagtcctga tgtacggtcc caagtcgaag tatgccggag agatcaacaa ggataactgg
      601 acagaggttc ttatgcgtac cgatgtacgc tgggggcact ccgagcctga cgccgatccc
      661 tgtgggtaca ggagcctgat ggtccttcag ctggccgagg agttctacgg tgacaaggac
      721 ctttatgcca gggcaatgga acaccccgag agagcggtac gtccgaaagc catcgacctg
      781 gtggccatgg tcgagagcgg agccatggat tacgccttcg agtacaaatc tgtagctgtc
      841 cagcacgggc tgaactacct tgaccttccc gacgagatca acctgaagga ccctggtttc
      901 gctgagaagt acgctgccgc ttcggtggag ctggcgggca aggagccggg acagaagatg
      961 accgtcaagg gtgaacctat cgtatacggg ctggtgatcc caaaggatgc cccgaacatg
     1021 gagggcgctt tgaaactggt tgagttcatt cttgcccagg atggcgggtt gaaggtattc
     1081 gaagagatgg gccaggacat cgtcggcccg cagaccatca cgcctgggga gaaggttcct
     1141 gcgggcgtag aagcactgct ggtggaatag ccgaagtcat aaagaagggg ccccggttca
     1201 ggccggggcc cctgtctcac ctgaactggt cctgtattca gggcaggtta tcagtcaaca
     1261 ccgatcatca cgttggaggc cttgatgaca gcgtaaactt ccttgccagg tttcaggccc
     1321 agcttatcgg cggaatcctt tgagatcatg gagacgatct cctctcctcc cttgagctcc
     1381 actatcacct cggtgttcac tgccccggcc ttgacttcct taaccttgcc cttcagggca
     1441 ttgcgggcgc tcaacttcat ttcatccacc tccgtctaca tacccataag ggtttatcat
     1501 gcaagttagc gatatgatct tatcatatat cagctcctgc agcaagcggt cagagttgtc
     1561 ggcttcagtt gcctgtcttg ccgtgtcccg caatgatcca tgccgcagcg agtcctgcgg
     1621 cgaatcccgc cgccatgttc aggtacaggg agaaaccgac ggtaactgca acaactgacc
     1681 actctcctgg gcctctcaca gatctggtga acttcgccat ctctacgccg accatgatca
     1741 tcattgctcc cacgatcgcc atggggaagg acctgaaaat ggtagctatg tgaccggcaa
     1801 agaagatgcc gagcagtatc tcaaggaagc cttcgatcac gttcgctcct cccgttcggg
     1861 cgccgtagta gtactgtccg gcaaggccac ctgccccgtg acaaaggggg ataccgccta
     1921 tgaacgggcc caggaggttc attacgccca tgttgatcga gagatgcctt gccgagactg
     1981 atcggtcggg ccagtacctg gatatcagtg ctgaagtagc gatgaccgcg ttggtagctg
     2041 tcaggggaat ttgcgagaac cctccgtccc tcatggaagg ccagaacagt tccagacgga
     2101 gggggtggag aggagggagt gtgaaggtga cggactggat ctgggacagg tcacccctga
     2161 aagccatcag aagcacgccg ccgaggacaa gaaccagcga agccggggcg aagcggttat
     2221 ccctcaggac cagtataagc agtatcgaaa ggccggccag gatccaggcg ccatcgatca
     2281 tccggaagcc ctgcatagcc aggagcaggc ccagcgagag ctggatcccc cggataaccg
     2341 cctggggggt gcgggatgcg atccatgtta tggcccccga gaagcccagg atcagccatg
     2401 taactcccat agcaaaggcc gacatgaaga ccttgtcggg ggtccatgcc tgggctatgg
     2461 ccattacggc gagaaccttc atgggctcta tgggcatggg gagccggtag atgatcccgg
     2521 tgaggatatt cgcaagtccc atgaacacca gcatccccgc agggtcgatg ccgcagacgg
     2581 ttatatagcc cacggccagg ggaagcagag tccccaggtc gcccagggac cccccgagct
     2641 cccggaggct gaattcgaag gagccgatct ttgccataat attctctccg catgctatga
     2701 cctgaaacag caagaacacc cgtgaatata gggt
//
LOCUS       DAOPKQ010000190         2719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_42723_length_2719_cov_3.871997, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000190 DAOPKQ010000000
VERSION     DAOPKQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42723_length_2719_cov_3.871997"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PK146_06455"
     CDS             <1..567
                     /locus_tag="PK146_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPE91915.1"
                     /translation="DVAAPWDPGKLRNALDANLPPSISVIRTAPVTEVFSARRSASWR
                     EYLYFVWKDHGCPPQFAPWVWKNTAPWDREAMRSLCSLLRGEHDFGAFCRKADRPPGA
                     RRTILSAAVSGKGPLLRFRIRGDSFLTNMIRIILGSMDLVASGKRDLTWFEGLLHGGD
                     RCQAGPTAPASGLFLASVGYSPSPWNDS"
     gene            676..1719
                     /locus_tag="PK146_06460"
     CDS             676..1719
                     /locus_tag="PK146_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPE91916.1"
                     /translation="MWGMDIGIDLGTANVLIFMKNSGVVLREPSVVAVDQNTDRILAV
                     GNEAKNMLGRTPGNVVAIRPLKDGVIADYTMTEAMLRYFLKKVITGFGRMMRRRVMIC
                     VPSGATDVERRAVLEAAVEIGAKEAFLIEEPMAAAIGANLDISEPRGNMVVDIGGGTT
                     DIAVLSYGGIVVTESLRVGGDRFDENITRFIRKEYNLAIGEQTAESIKINIANCFSSQ
                     QKEETMAIKGRDLVHGLPRQIEITDRTVAKAIEETVNMIIEGIRQVLEKTPPELSADI
                     IDRGITLTGGGALLKGLPELITKKTLIPAYVADSPLECVAIGTGKALDDIDKLRSSGS
                     LIGASRRRERRVW"
     gene            1721..2200
                     /locus_tag="PK146_06465"
     CDS             1721..2200
                     /locus_tag="PK146_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPE91917.1"
                     /translation="MVSGSDGLSEVEIKGTGELLYKLWWKEGLLFSCLGCGRCCRGEP
                     GSVWLSPREAEDIAACLGMERERFLATYVTERRGRKTLRERLNGDCVMYEREGARCRI
                     YGVRPLQCRLFPFWPSVLRTEESWEDHSRECPGMGQGRHWPAEEIAALLEMSPFQDL"
     gene            2341..>2719
                     /locus_tag="PK146_06470"
     CDS             2341..>2719
                     /locus_tag="PK146_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HPE91918.1"
                     /translation="MAKVDRRVEAQNVFDEIRKWLLRDGFDIVIDMEKSKGSHMINKV
                     NGEDWLDFYTFFASAPFGMNHPKLDNPEFKETIFRAAINKVASSDIYTEQMAQFVKTF
                     GEVAVPEGFNHVFFIDYGTLAVEN"
BASE COUNT          621 a          726 c          829 g          543 t
ORIGIN      
        1 gacgttgcag ccccctggga tcccggaaag ctgaggaacg ccctggacgc caacctcccg
       61 ccctccatca gcgtgatccg cacggcaccc gtaacagaag ttttcagcgc caggcgaagc
      121 gcgtcctgga gggagtacct ctattttgta tggaaggatc acggatgccc accccagttt
      181 gccccatggg tctggaagaa caccgccccc tgggacaggg aggccatgcg gagcctgtgc
      241 tctctcctca ggggtgaaca cgacttcggc gccttctgcc gaaaggctga ccgcccaccg
      301 ggcgcaagaa ggacgatact gtcggcggcg gtctccggga aggggcccct actccgtttc
      361 aggataagag gggacagttt ccttacgaac atgataagga tcattttggg gtccatggac
      421 ctggtcgcct cagggaaacg ggatctcacc tggttcgagg ggcttctcca cggaggtgac
      481 aggtgccagg caggacccac cgcgcctgca tcgggtctct tccttgccag tgtgggttac
      541 agtccttccc catggaatga ttcttgaggg taacggcctt tataggatat agtctaacgg
      601 tgggactgag gtaagggtct tttcgacgct tttttattcg catgtccggt cacagttcga
      661 aggaggtagc cttgcatgtg gggtatggat attgggatcg atctggggac ggcgaacgtc
      721 ctgatcttca tgaagaacag cggtgtggtc ctcagggaac cctccgtagt ggccgtggac
      781 cagaacaccg acaggatcct ggccgtgggc aacgaggcga agaacatgct cggacggacc
      841 cccggcaacg tggtggccat caggccgctg aaggatggcg taatcgccga ctacaccatg
      901 acggaggcaa tgctccggta cttcctcaag aaggtcatca ccggtttcgg gagaatgatg
      961 aggcgcaggg ttatgatatg cgttccctcg ggtgctactg acgtggagag aagggccgtc
     1021 ctcgaggctg cggtggagat cggagccaag gaggccttcc ttatcgagga gcccatggcc
     1081 gctgccatcg gggcaaacct tgatatctcc gaacccaggg ggaacatggt cgtcgatatc
     1141 ggtggcggca ccaccgacat agccgtcctc tcctacggtg gaattgtggt gaccgaatca
     1201 ctcagagtgg gtggagaccg tttcgacgag aacattaccc gcttcataag gaaggagtac
     1261 aacctcgcca tcggcgagca gacggcggag tcgatcaaga taaacatagc caactgtttc
     1321 tcctcgcagc aaaaagaaga gaccatggcc ataaaggggc gtgatcttgt ccacggcctt
     1381 ccgcggcaga tcgagatcac cgaccgtacc gtggccaagg cgatcgagga gacggtgaac
     1441 atgatcatcg agggtatccg tcaggtcctc gagaagaccc cccccgaact ttcagcggac
     1501 atcatagaca ggggtatcac tcttactggc ggaggtgccc tcctgaaggg cctccccgaa
     1561 ctgatcacaa agaagaccct gatacccgcc tacgtggcgg actcccccct ggaatgcgtc
     1621 gccatcggga ccggcaaggc actggacgat attgacaagt tgcgttccag cggttcactt
     1681 atcggtgctt cccgtcgcag ggagagaagg gtctggtgac atggtatccg ggtctgacgg
     1741 actttcggag gttgaaatta agggtactgg tgagttacta tataaactgt ggtggaaaga
     1801 gggtttactt ttctcctgcc tgggctgtgg aagatgctgt cggggcgaac ccggttcggt
     1861 ctggctctca ccccgggagg cagaggacat cgcagcctgt ctggggatgg agagagagcg
     1921 cttcctcgcc acttatgtga ccgaacgcag ggggaggaag actctcaggg aacgccttaa
     1981 cggcgattgt gtcatgtatg aaagggaagg cgccaggtgc cgtatctacg gtgtaagacc
     2041 ccttcagtgc aggctcttcc ccttctggcc cagcgtcctc agaacagagg agagctggga
     2101 ggaccactcc cgggagtgtc cggggatggg ccagggtcgg cactggccgg cggaagagat
     2161 agcagccctg ctggagatgt cgccctttca ggatctttga ccctttttcc cggcagttcc
     2221 gggaacttgt cctggtggag gaatagtgaa ataataaaca agtaatactc acggtctcga
     2281 aacaggaaag agggatacca gcgggcatcc caagacaaat aaaacaggga ggtagctcga
     2341 atggcaaagg tcgacagaag agttgaagcg cagaacgttt tcgacgagat caggaagtgg
     2401 cttctccggg acggtttcga tatcgtcatt gacatggaga agtcaaaggg ttcccacatg
     2461 atcaacaagg taaacggcga ggactggctt gatttctaca ccttctttgc ttcagccccc
     2521 ttcggaatga atcaccccaa gctcgacaac cctgaattca aggagaccat cttcagggca
     2581 gccatcaaca aggtggcaag ctccgacatc tacactgaac agatggccca gttcgtaaag
     2641 accttcggag aagtggccgt tcccgagggt ttcaaccacg ttttcttcat cgactacggg
     2701 accctcgccg tggagaaca
//
LOCUS       DAOPKQ010000191         2715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_42820_length_2715_cov_4.056767, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000191 DAOPKQ010000000
VERSION     DAOPKQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42820_length_2715_cov_4.056767"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK146_06475"
     CDS             <1..316
                     /locus_tag="PK146_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE91919.1"
                     /translation="FSRRRDIMTGLLREMPLISFEEPRGAFYVFVNVLEALGGTSGGK
                     ILENDGTFCEALLEREFVAAVPGTAFLAPGYIRLSYANSEGEIREGMSRLKRFLQGIE
                     AR"
     gene            complement(369..926)
                     /gene="frr"
                     /locus_tag="PK146_06480"
     CDS             complement(369..926)
                     /gene="frr"
                     /locus_tag="PK146_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPE91920.1"
                     /translation="MADQEIRKMKEKMDKAVEHLKKELLGIRTGRAHPALVSDIKVDY
                     YGNPTPMSQVGSISTPDARQILITLWDKNAVKAAEKAIQASSLGINPSVDGENIRLII
                     PELTGERRIELSKLVKKSAEDARIAIRNLRRESNDALKKMEKDGSLSEDSLHDYLETV
                     QKTTDEFIKKVDGIAAEKEKEILED"
     gene            complement(932..1654)
                     /gene="pyrH"
                     /locus_tag="PK146_06485"
     CDS             complement(932..1654)
                     /gene="pyrH"
                     /locus_tag="PK146_06485"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPE91921.1"
                     /translation="MASYHRILLKLSGEVLAGDSSFGLDMNAISKICREIASLTENGL
                     EIAMVVGGGNIFRGAQSGEGYLDRTQGDYMGMLATVINSLALQSVLERLGVPTRVQTA
                     IEMRELAEPFIRRKALRHLEKGRIVIFAAGTGSPFFSTDTAAALRAAEISADCLLKAT
                     KVDGIYDKDPADNPDARLLPRITYVEALQKQLKVMDAAAFSLCMENRIPILVANILKE
                     GTLRDILIRGKQVGTIVHFEKE"
     gene            complement(1723..2316)
                     /gene="tsf"
                     /locus_tag="PK146_06490"
     CDS             complement(1723..2316)
                     /gene="tsf"
                     /locus_tag="PK146_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPE91922.1"
                     /translation="MSVDMNAVKELRYRSGAGVLDCRKALEECCNDIEKAVDHLREKG
                     LAKAAKKAGRTASEGRVFSYIHTNGKIGTLVEINCETDFVARTDEFQELGKEIAMQIA
                     AAAPLYLTTEEVPQGDLEREKEIYRQQALDEGKPEKIVDKIAEGRIAKFFEESCLMEQ
                     KYIRDPEKKIKDLVVQEIAKMGENIVVRRFARFSIGE"
     gene            complement(2323..>2715)
                     /gene="rpsB"
                     /locus_tag="PK146_06495"
     CDS             complement(2323..>2715)
                     /gene="rpsB"
                     /locus_tag="PK146_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPE91923.1"
                     /translation="MKDVPDALVVVDPKREDIAVLEARKLGIPIVGIVDTNCDPDLID
                     YPIPGNDDAIRAIKLIIGLLASAVIEGRQGVDNVSGESAEDSEPDVEEAVDPSKLEIT
                     EKLHENYGAVEEVIVDKELLERKGWKEA"
BASE COUNT          510 a          819 c          698 g          688 t
ORIGIN      
        1 gttctcccgg cgacgggata tcatgaccgg ccttctcagg gagatgcctt tgatatcctt
       61 cgaggaaccc cggggggcct tctacgtctt tgtcaacgtc ctggaggccc tcggtggtac
      121 ctccggaggg aagatcctag aaaacgacgg cacgttctgc gaggctctcc ttgaaaggga
      181 gttcgttgcg gcagtccccg ggaccgcttt cctggcccct ggttatattc ggctcagcta
      241 cgccaactcc gagggggaga tacgagaggg gatgtccagg ctgaaaagat tccttcaagg
      301 aatagaggcc agataaaaga aggggggcgc cgtcaagggc gccccccttc tacctggatc
      361 agggcccctc agtcttccag gatctctttt tccttttctg cggcgatccc gtctaccttt
      421 ttaatgaact cgtcggtggt tttctggacc gtttcgaggt agtcgtgcag cgagtcctcg
      481 ctaaggctcc catccttttc catcttcttg agagcatcgt tcgattctcg cctcaggttc
      541 ctgatggcga tcctggcatc ctcggcagac ttcttgacca gttttgaaag ctcgatccgc
      601 ctctcgccgg tcagctccgg gataatgagc ctgatattct cgccgtcgac cgacgggttg
      661 ataccgagcg atgaagcctg tatcgccttc tctgccgctt tcacggcgtt tttgtcccaa
      721 agggtgataa gtatctgcct ggcatcgggc gtggaaatgg agcccacctg cgacatgggg
      781 gtcgggttcc cgtagtagtc caccttgatg tcgctcacca gggcagggtg agcccttcct
      841 gtcctgatgc ccaggagctc cttcttaagg tgctccacgg ctttatccat cttctctttc
      901 atcttcctga tctcctgatc agccattctc atcactcctt ttcgaaatga acgatggtgc
      961 cgacctgttt gccgcggatc aggatgtccc ggagggtacc ctccttgagg atgttcgcca
     1021 caaggatggg gatccggttc tccatgcaga gcgaaaaggc ggccgcatcc atgaccttga
     1081 gctgcttctg gagcgcttca acataggtta tccgggggag cagtcttgcg tcgggattgt
     1141 ccgccgggtc cttatcatat ataccatcga cctttgtggc cttcaataga caatcggcgg
     1201 atatctctgc cgctctcagt gcagctgcgg tatcggtcga gaagaagggc gaccccgttc
     1261 cggccgcaaa gatcactatc cttcccttct caaggtgcct gagagccttt ctcctgataa
     1321 agggttcggc cagttctctc atctctatgg ccgtctgaac cctggtggga actccaagac
     1381 gttccaggac gctctggagg gcaagcgaat tgatgacagt tgccagcatc cccatgtaat
     1441 cgccctgggt tctgtcaagg taaccctctc cggactgggc gcccctgaaa atattacctc
     1501 cgccgactac catggctatc tccagtccgt tctccgtcag ggaggctatc tccctgcaga
     1561 tcttgctaat ggcgttcatg tccagcccga aggaggaatc accggccagg acttctccgg
     1621 aaagtttaag gaggatcctg tgatatgagg ccatgaagcg aacacctcct ctaaaataaa
     1681 aaggcaaaaa aagggcgggg tatcaccccg cccctgactg ggttattcac ctattgaaaa
     1741 acgtgcgaat ctcctcacta cgatgttctc gcccatcttg gctatctcct ggaccacgag
     1801 gtccttgatc ttcttctcgg gatcccggat gtacttctgc tccataaggc agctctcttc
     1861 aaagaatttg gctatgcgcc cctcggcgat cttgtccacg atcttttcgg gcttaccctc
     1921 gtcaagggcc tgctgcctgt agatctcctt ctccctctca agatccccct gggggacttc
     1981 ctcggtggtc aagtaaagtg gagcggctgc ggcgatctgc attgcgatct ccttgccaag
     2041 ctcctggaat tcgtcggtcc ttgccacaaa atcggtctcg cagttgatct ccaccagggt
     2101 accgatcttc ccgttggtgt gtatgtaact gaagaccctg ccctcggaag cagtcctgcc
     2161 tgccttcttt gctgctttgg caaggccctt ctcccggagg tgatccacgg ccttctcaat
     2221 atcgttgcag cactcctcca gggccttgcg gcaatcaaga acacccgccc cggagcggta
     2281 tctgagttcc tttacggcat tcatgtccac tgacattacg aactaggcct ccttccagcc
     2341 ttttctctcc aaaagttcct tgtccactat cacttcttca accgccccgt agttctcatg
     2401 gagcttctcg gttatctcaa gcttggaggg atcgacggcc tcctccacat cgggctcgga
     2461 gtcctcggca ctctccccgg aaacattgtc gacaccctgc cttccttcga tgacggcaga
     2521 tgccagcagg ccgatgataa gctttattgc cctgatggcg tcatcgtttc ccgggatcgg
     2581 gtagtcgatc aggtcggggt cgcagttcgt gtccacgatc cccacgatgg ggataccgag
     2641 cttcctcgct tcaagtaccg cgatatcctc ccgcttcgga tctacgacga caagggcgtc
     2701 gggaacatct ttcat
//
LOCUS       DAOPKQ010000192         2699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_43147_length_2699_cov_4.348714, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000192 DAOPKQ010000000
VERSION     DAOPKQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43147_length_2699_cov_4.348714"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /gene="lon"
                     /locus_tag="PK146_06500"
     CDS             <1..1324
                     /gene="lon"
                     /locus_tag="PK146_06500"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPE91924.1"
                     /translation="VLCFAGPPGVGKTSLGRSIARATGRKFVNMSLGGIRDEAEIRGH
                     RRTYIGALPGRIIQKLRQAGTCNPVMLLDEIDKIGLDFRGDPAAALLEVLDPEQNNAF
                     TDHFLEVPFNLGNVLFITTANTTHTIPRPLLDRMEVIRLPGYVTEEKQQIARKHLIPK
                     VLYEHGLKEGQITFTSASVRKIISDYTREAGVRELERQISKVTRKITRKIVESEETSR
                     GAKRKKKNFRVTPSDLLPYLGVPRHTGGVHLPASDQTGASIGLAWTETGGDILVIESV
                     RIPGKGSIVLTGNLGEIMQESGQTALGFLKAHCEELGISSIDWDKTDIHVHVPEGAIP
                     KDGPSAGITLAVSIYSALSGRPVRNDVAMTGEVTLRGDVLPVGGIREKVLAAKRYGFR
                     NVILPEGNRLEAGEMPRWVLRGLDLVFVSGLEEVFQYSMSRERDNGEH"
     gene            1311..1931
                     /gene="yihA"
                     /locus_tag="PK146_06505"
     CDS             1311..1931
                     /gene="yihA"
                     /locus_tag="PK146_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPE91925.1"
                     /translation="MGSTRRKRWEAFLAATCFSSDQFPPSRGPEIAFAGRSNVGKSTF
                     INGLIRTPLAHVSSTPGKTRSINFYRVDHGEGFCLVDLPGFGFANRARSERDAWAKLI
                     EKYITRRESLALLVHLVDFRHGLLENDRKLQEWAIEQSVPVQVVFTKIDKIPRGRWPS
                     FLRSYSVPQVSTVTDPFLVSMEKGWGAGDFILFLETYLKRVWAERR"
     gene            1998..>2699
                     /locus_tag="PK146_06510"
     CDS             1998..>2699
                     /locus_tag="PK146_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPE91926.1"
                     /translation="MASSKRTLGKTYDPAPIEGKWYRCWNDRGMFRGQIDSKKPHFSV
                     VIPPPNVTGSLHMGHALNNTLQDISCRFMRMCGFNVLWLPGTDHAGIATQNVVEKYLS
                     SQGISRHDLGRERFVEQIWEWKKEYGGRIINQLKRLGASCDWDRERFTMDEGLSRAVR
                     AVFVRLYKEGLIYRGNYIVNWCPRCLTALSDLEVEHRDEMGVLYHVRYPFVESDGHII
                     VATTRPETILGDVAVA"
BASE COUNT          649 a          708 c          799 g          543 t
ORIGIN      
        1 ggtgctgtgc ttcgccggac cgccgggggt agggaagacc tccctgggac gctccatagc
       61 aagggcgacg ggcagaaagt tcgtgaacat gtctttgggc ggtatcaggg atgaggctga
      121 gatccgtggt cacaggagaa cctatatcgg agcgctgccg ggcagaatta tccagaagct
      181 cagacaggcc ggcacgtgca accccgtcat gctccttgac gagatcgaca agataggcct
      241 ggacttccgg ggtgacccgg ctgcagcgct tctggaagtg cttgatcccg agcagaacaa
      301 cgcctttacg gaccacttcc ttgaggtgcc cttcaacctg gggaacgtcc tcttcatcac
      361 aactgccaat acgacccaca cgatacccag gcctctgttg gaccgtatgg aggtcatccg
      421 gcttcccggt tacgtcacgg aggagaagca acagatagcc aggaaacacc ttatacccaa
      481 ggtcctctac gaacacggcc tcaaggaagg tcagatcaca ttcacttcag cttcggtcag
      541 aaagataatt tccgattata cgagagaggc cggggtacgg gagctcgaaa gacagatctc
      601 caaggtaacc agaaagatca cccgcaagat cgtcgagtcg gaagagacat cccgtggtgc
      661 aaagagaaag aagaagaact tcagggtcac tccgtcggac cttctccctt accttggagt
      721 gccccgtcat accggaggag tccacctccc cgcttcggat cagaccggtg cctccatcgg
      781 gcttgcatgg accgagacgg gaggggatat tcttgtaatt gaaagcgtac gcatccccgg
      841 gaagggcagt atagtcctca cgggaaacct gggagagatc atgcaggaat caggccagac
      901 tgcactgggc ttcctgaagg cccactgtga ggaactgggg atctcctcta tcgattggga
      961 caagaccgat atacatgttc acgtcccgga aggggccatc cccaaggatg gaccttcagc
     1021 gggtatcacc ctggccgttt cgatctactc ggctctttcg gggagacccg tgcgcaacga
     1081 tgttgcaatg accggtgagg tcacattgag gggcgacgtt ctgcccgtcg gcgggataag
     1141 ggagaaggtc cttgccgcca aaaggtacgg attcaggaac gttatccttc cggaaggcaa
     1201 ccgtctcgag gcgggggaga tgcccaggtg ggtcctgcgg gggctggacc ttgtatttgt
     1261 cagtggactg gaagaagttt tccagtattc catgtcaaga gagagggata atggggagca
     1321 ctagaagaaa gaggtgggag gctttccttg ccgcgacctg tttctccagt gaccagttcc
     1381 caccttccag gggaccggag atcgccttcg ccggcaggtc gaacgtaggg aaatcaacct
     1441 ttatcaacgg tctgatccgg acccccctgg cccatgtcag ttccactccg ggcaagaccc
     1501 gaagcatcaa tttttaccgg gtcgatcacg gagagggatt ctgccttgtc gatcttccgg
     1561 gtttcggatt tgccaacagg gctcgttcag agagagatgc ctgggcgaag ctaatagaga
     1621 agtacataac ccgaagagaa agtctggccc tccttgtcca ccttgttgat ttcagacatg
     1681 gtttgcttga aaatgacagg aagctccagg aatgggccat agaacaaagc gttcctgtcc
     1741 aggtggtgtt cacaaagatc gacaagatcc ccagggggag atggccctcc ttcctgagga
     1801 gctacagcgt ccctcaggtc tccaccgtta ccgacccctt cctcgtttcc atggagaagg
     1861 gatggggagc cggggatttc attctgttcc tggagactta cctgaaaagg gtctgggcgg
     1921 aaaggcgtta gggtaaccgc ccgggaagtg aaggaaatgg ccgcgtacaa acaccggaaa
     1981 ggtcaaggag gaaaatcatg gcatcttcga agagaacgct gggcaagact tatgatccgg
     2041 cccccattga gggtaaatgg taccgctgct ggaacgacag aggaatgttc agggggcaga
     2101 tagacagcaa aaagccccat ttcagcgtgg tcatccctcc cccgaacgta acggggtccc
     2161 tccacatggg gcacgccctt aacaataccc tgcaggacat atcatgccgt ttcatgagga
     2221 tgtgtggttt caacgtcctc tggcttccgg ggaccgacca tgccggaata gctacccaga
     2281 acgttgtaga aaagtatctt tcctcccagg gcatttccag acacgacctt ggcagggaga
     2341 ggttcgtgga acagatctgg gaatggaaga aggaatacgg gggcaggatc ataaaccagt
     2401 taaaacgatt gggcgcctcc tgcgactggg atagggagcg cttcacaatg gatgagggac
     2461 tctcaagggc cgtcagggct gttttcgtaa ggctttacaa ggaaggtctc atatacaggg
     2521 gcaactacat cgtgaactgg tgccccaggt gcctgacagc cctctccgac cttgaggtgg
     2581 agcacaggga tgagatgggc gtcctttacc atgtgcggta ccccttcgtg gagagcgatg
     2641 gtcacataat cgtcgctacc acaaggcccg agactattct tggcgatgta gccgtggca
//
LOCUS       DAOPKQ010000193         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_43499_length_2683_cov_4.516362, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000193 DAOPKQ010000000
VERSION     DAOPKQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43499_length_2683_cov_4.516362"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK146_06515"
     CDS             <1..462
                     /locus_tag="PK146_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPE91927.1"
                     /translation="NPLAHLDPIGALMLLIFRFGWAKPVPIEPRHFRHPRRDLVIVSL
                     AGSTGNLLTAVACGLLVRLFPGPFIAIPALGRFIVLMVLINLGLGVFNLIPIPPLDGS
                     KLLYVFLPASWLRGFFWLERYGFFVLMALLVLGVVQAIISPIIMTLFHLIL"
     gene            479..1261
                     /gene="surE"
                     /locus_tag="PK146_06520"
     CDS             479..1261
                     /gene="surE"
                     /locus_tag="PK146_06520"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPE91928.1"
                     /translation="MKVLVSNDDGVFAPGIIVLTRKLHDSGYEPCVVAPDRERSSVGH
                     AITLNRPLRLWEVEGGGYPGCVKVYASDGTPSDCVVLGLEAVAPDSAMVISGVNRGPN
                     LADDLTYSGTVSAAMEGHFLGKPSIAVSLNWSPGDGQEHYDTAASAAVSLLRSFVEFP
                     FPEGVLLNVNVPNIAGDDLKGFRVTRKGVRIYREKVTPLKDPKGRPYYWVSGRPEDVM
                     EEGSDVRAVAEGFVSITPIHMDMTHYPSIELLREAGFEERQR"
     gene            1669..>2683
                     /locus_tag="PK146_06525"
     CDS             1669..>2683
                     /locus_tag="PK146_06525"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPE91929.1"
                     /translation="MEYKGKVVLAYSGGLDTSVAIKWLGDQGYDVVTLTADVGQTIDL
                     EGVKEKALKVGAVNAYVMDLRQEFVETMVWPALKANALYQGKYPLNSALSRPLIAKYL
                     AWVADMEGAVAVAHGCTGKGQDQVRIEVCTNALNPDLKVIAPVRDWHFSRDVEIEYAK
                     ENNIPVPVTLASPYSIDGNLWGRSIECGPLEDPWTEPPSDAFTMTVDPWEAPDEQEYI
                     EVSFEKGVPFAINGKEMDGVTLIAELNKIAGGHGVGRIDMLEDRLVGFKSREVYECPA
                     TVVLLQAHQALESMTLTKDIIAAKAELSLKYAELTYIGYWFSPLKKAFDAFFDSIQEH
                     VTGT"
BASE COUNT          567 a          732 c          824 g          560 t
ORIGIN      
        1 aaccccctgg ctcaccttga tcccatcgga gcccttatgc tgctcatctt ccgattcggg
       61 tgggccaagc cggtccctat cgaaccgagg cacttcaggc acccaagacg ggatctggtg
      121 atcgtgtccc ttgcgggttc aactggcaac ctgctcaccg cagtcgcctg cgggctgctg
      181 gtgcgccttt tccccggtcc cttcatcgcc attccggccc ttggaaggtt catagtcctg
      241 atggtcctga tcaatctagg cctgggggtc ttcaacctga tccccattcc tcccctcgac
      301 ggctccaaac tactttacgt gttcctgccg gcgtcgtggc tcagggggtt cttctggctg
      361 gagaggtacg ggttcttcgt cctgatggcc ctgcttgtcc tcggagtggt ccaggccatc
      421 atcagcccta tcataatgac cctttttcac ctgatactct aaaggagagt gcagcctctt
      481 gaaagttctc gtctcaaacg acgacggcgt ttttgcaccg ggaataatcg tcctgaccag
      541 gaagctccac gatagcggtt atgaaccgtg cgtagtggct cctgacaggg agcgcagcag
      601 cgtgggtcac gcgataaccc tcaacagacc tcttcgtcta tgggaggtcg agggcggtgg
      661 atatccgggg tgcgtcaagg tctacgccag cgacgggacc ccgtcggatt gtgttgtcct
      721 tggcctggag gcagttgccc ccgattcggc aatggtcatc tccggggtca accgggggcc
      781 caaccttgcc gacgacctga cctattcggg gacggtatcg gcggccatgg agggtcactt
      841 cctggggaaa ccttccatcg cagtctccct caactggtcc cccggcgacg ggcaggagca
      901 ttacgataca gcggcttctg ctgcggtgtc ccttctaagg agtttcgtgg agttcccctt
      961 tcctgaaggg gtcctgctta atgtcaacgt tcccaatatt gctggagacg accttaaggg
     1021 cttcagggtc acaaggaaag gggtccgtat ctacagggag aaggtgaccc ccctgaagga
     1081 tccgaagggg cggccatact actgggtatc gggaagaccg gaggatgtaa tggaggaggg
     1141 cagcgatgta agagcggtcg ctgaagggtt cgtctccatc accccgatac acatggacat
     1201 gacccactac ccctccatag agctccttcg tgaggctggg ttcgaggaac ggcagcgttg
     1261 acattctccg ggggtctgtg tataatctcc gctatcggtt ggaacgatct caacaaggtt
     1321 ggtgcggtta tggcagttac ccgtatgaga cgagcgagac gaataagacc tgttctatct
     1381 gcgtcggagt tcgcgcatga ggcggcgatg acggggaaga gtaggtcgtg tgctcgtcga
     1441 cagagagggg atgcaaaccg ctgaaagcgt tcctcgtcga gaccgactga atagagcccc
     1501 cgagccggcc cccgaagaaa gccctcttgc agggcaagta ggggggaacg gtttaaagcc
     1561 gtgagagtag gcctgggggc cgaaaagtag ggtggcaccg cgagtcaagc tcgccccttc
     1621 gaaagaaggg gcgagctttt ttgttactta caaaaggagg ggacaggaat ggaatacaag
     1681 ggaaaggtag ttcttgctta cagcggcggt cttgatacat cggtggcgat caagtggctt
     1741 ggggatcagg ggtatgacgt agtgaccctt acggctgacg tggggcagac catcgatctt
     1801 gagggggtca aggagaaggc cctgaaggtt ggagccgtca atgcatacgt gatggacctc
     1861 aggcaggagt tcgtggaaac catggtctgg cctgccctga aagccaacgc cctctaccag
     1921 ggcaagtacc cccttaattc tgccctttca aggcctctga tcgcaaagta ccttgcatgg
     1981 gtcgccgata tggagggcgc cgttgcggta gcccatggat gtacaggcaa agggcaggac
     2041 caggtaagga tagaggtatg caccaatgcc ctgaaccccg acctgaaggt catagctcct
     2101 gtcagggact ggcacttctc gagggacgtc gagatcgaat acgccaaaga gaacaacatc
     2161 cccgttccgg tcacgctggc gagcccctac tccattgacg gcaatctctg gggcaggtcc
     2221 atcgagtgcg gtcctctgga ggacccctgg acggaacctc ccagcgatgc ttttacaatg
     2281 accgttgacc cctgggaggc ccccgacgag caggagtaca ttgaggtatc ctttgaaaag
     2341 ggggtcccct ttgctatcaa cggcaaggag atggacggcg taacgctgat agccgagctg
     2401 aacaagatcg ccggcggtca cggagtgggg cgcatagata tgctggagga caggctggtg
     2461 gggttcaaga gcagggaggt ctacgaatgc ccagcgacgg tcgtccttct ccaggcgcac
     2521 caggccctgg agagcatgac cctgaccaag gacataatag ccgcaaaggc ggagctgtcg
     2581 ctcaagtacg cggagctgac ctatatcgga tattggttct cgccgctgaa aaaggccttt
     2641 gatgccttct tcgattctat ccaggagcac gtaacaggca ctg
//
LOCUS       DAOPKQ010000194         2671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_43785_length_2671_cov_3.725535, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000194 DAOPKQ010000000
VERSION     DAOPKQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43785_length_2671_cov_3.725535"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK146_06530"
     CDS             <1..334
                     /locus_tag="PK146_06530"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HPE91930.1"
                     /translation="GGVRELGRRIERDRTRDWRIFNLQYVLFRVFLLGPERSREIMEE
                     DPPAWLSLTATGVGGIRSGGDVSVKCLHLQAASMIGMGWHPGEDWLCERIGVQECDDP
                     LEWPCTTG"
     gene            319..1239
                     /locus_tag="PK146_06535"
     CDS             319..1239
                     /locus_tag="PK146_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HPE91931.1"
                     /translation="MHDRLRFGAVDLGSNSVRCLAVSVSGGVLEYVASGVWISRVTEG
                     IREGEYAVGGKALERTLEAVGKAFQLLERSGVTGGHISFCATESLRSAINSGEVVARM
                     ESLTGLTPRVLDPEEEARLGRKGASLGIPGAGWVFDLGGGSLDIGTPHRSFSFPLGAV
                     RMTALFGEEPEAVMEEVRSVIGKSLEGGPGNLVGVGGTSSAAVMMLEMVRVDDYRPSR
                     LHGRKVRSGDLDELVRRISATPLEERAMITGLEKGRADIIVTGLSVARALLGLMGVDE
                     YTHSETDLLWALCNDMAAERGSEALSVRMP"
     gene            1262..2671
                     /gene="argH"
                     /locus_tag="PK146_06540"
     CDS             1262..2671
                     /gene="argH"
                     /locus_tag="PK146_06540"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPE91932.1"
                     /translation="MWHGRFREDTAEIVQHFTQSLDLDWRLAPYDVYGSIAHVRMLEH
                     VGLLEQREAAVIEDGLRQVLQEIQEGHLKPLESMEDVHMNIEARLTELIGPLGAKLHT
                     GRSRNDQSATAVRLYLRKEMYSVGDALITLLDALLDRATAHRHIIIPGYTHLQQAQPV
                     SMGHYWLAHFWALARDFRRVLFALESVNECPLGSGALAGSTLPLDRGFTSIMLGFDRT
                     SDNSMDSVAHRDHLLDCQYAMTVLMIHASRMAEDMVIYNSREFGWVILPDAFCTGSSM
                     MPQKKNPDVLELVRGRTGRVIGDMVDLLTIIKGLPMTYNRDMQEDKRALWSSVQIARD
                     VLDVLPQLIARIEVDQERAVKAFEDGFALATDVAEYLVMRGVPFRLAHEQVGGVVKWC
                     LDRKRTFLSLSAGEWKELVPSAGDDLMAILNIESSVSRRNTQGGTSFKQVALQIERGM
                     ETLEVFRKKLASYPARFAL"
BASE COUNT          550 a          702 c          907 g          512 t
ORIGIN      
        1 cggaggggta agggagctgg gtcgtcggat agaacgtgac aggacccggg actggaggat
       61 cttcaatctt cagtacgtgc ttttcagggt ttttcttctg ggtccagaaa ggtcccggga
      121 gataatggag gaagatcccc cggcctggct atcccttacc gctacaggcg tagggggcat
      181 caggtcaggc ggggacgttt cggtaaaatg ccttcacctg caggctgcct ccatgatagg
      241 gatggggtgg caccccgggg aggattggct ctgcgaaagg atcggggtgc aggagtgtga
      301 tgaccccctg gagtggccat gcacgacagg ctaaggttcg gtgccgtcga tctgggttcc
      361 aactccgtcc ggtgtcttgc cgtttcggta tcgggagggg tgctggagta cgttgcctcg
      421 ggggtatgga tatcaagagt gaccgagggc atccgggaag gagagtacgc cgtcggagga
      481 aaggcccttg aaaggaccct ggaggccgtc gggaaggcct ttcagctcct cgaacgttcc
      541 ggcgtcaccg gtgggcatat atctttctgt gcgacagaaa gcctgaggag tgccatcaac
      601 tccggcgaag tggtcgccag gatggagtca ctgacggggc taactcccag ggtgctggat
      661 cccgaagagg aggcccggct cggcaggaaa ggggcctctc tggggatccc cggggcggga
      721 tgggtctttg acctcggcgg gggaagcctg gatatcggga ccccccacag gtccttctct
      781 ttccccttgg gggccgtaag aatgaccgcc ctttttggtg aggaacccga ggcagtaatg
      841 gaagaggtca ggagtgtcat cgggaaaagt ctagaaggcg gcccgggtaa tcttgtgggg
      901 gtgggtggca cttcttcggc agcggtaatg atgttggaga tggttcgcgt ggatgattac
      961 cgcccctccc ggcttcacgg acggaaggtg aggtccggtg acctggatga actggttcgg
     1021 aggatctcgg cgacacccct tgaggaaagg gcgatgataa cgggccttga aaaggggagg
     1081 gccgacatca tcgtgacggg gctttcggtg gcaagagccc ttctggggct tatgggggtt
     1141 gatgaatata cccacagcga gacggacctt ctgtgggcgc tttgcaatga tatggcggca
     1201 gagaggggat ctgaggccct ctcagtcagg atgccgtaaa gggaccggga ggtagatctt
     1261 catgtggcac ggacgattca gggaagacac ggcagagata gtccagcact tcacccagtc
     1321 gctcgacctg gactggcggc ttgcccctta tgatgtttac gggagcatcg cccacgtgag
     1381 aatgcttgaa cacgtcggtc tgcttgaaca gcgggaggcc gcagttattg aggacggcct
     1441 tagacaggta ctgcaggaga tccaggaggg ccatctcaag cccctggagt cgatggaaga
     1501 cgtccacatg aacatagagg cgaggctcac ggagctgatc ggccctctcg gagccaagct
     1561 gcacaccggg aggagccgca acgaccagtc ggccacggcg gtcaggctct acctgaggaa
     1621 agagatgtac tccgtgggcg acgcccttat taccctgctt gacgctctgc tggacagggc
     1681 cacagcccac aggcacatca taatcccggg ctacacacac cttcagcagg ctcagcccgt
     1741 aagcatggga cactactggc tggctcactt ctgggctctc gccagggatt tccgcagggt
     1801 tctgttcgcc ctcgagtcgg tcaacgaatg tcccctgggc agcggggccc tggcagggtc
     1861 aacgctcccc ctcgaccggg ggttcaccag catcatgctc ggattcgaca ggaccagcga
     1921 caacagcatg gactccgtgg ctcacaggga tcacctcctt gactgccagt acgcaatgac
     1981 ggtcctgatg atccatgcaa gcaggatggc ggaggacatg gtcatctaca acagccggga
     2041 gttcgggtgg gtgatcctcc ccgatgcctt ctgcacaggg tccagcatga tgccccagaa
     2101 gaagaacccc gacgtgcttg aactggtgcg ggggcggacg gggagggtca tcggcgacat
     2161 ggtggacctt ctcaccatta tcaaggggct tcccatgacc tacaacaggg acatgcagga
     2221 ggacaagaga gctctctggt catcggtgca gatagccagg gatgtcctcg acgttctccc
     2281 ccagctgata gcccgcatag aggtggatca ggagagggcg gtcaaggcct ttgaggacgg
     2341 cttcgccctg gcgacggacg tggccgagta tctggtcatg aggggcgtcc ccttcaggct
     2401 cgcccacgag caggtaggag gagtggtcaa gtggtgcctt gaccggaaga ggacgtttct
     2461 gagcctttca gcgggggagt ggaaggagct tgttccctct gcgggcgacg acctgatggc
     2521 aatcctgaac atcgagagct ccgtctcaag gaggaacaca cagggcggga cctcgttcaa
     2581 acaggttgcg ctgcagatcg agaggggcat ggagacactg gaggtattcc gcaagaaact
     2641 tgcctcctac ccggcgaggt ttgcattgta a
//
LOCUS       DAOPKQ010000195         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_45015_length_2621_cov_4.107171, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000195 DAOPKQ010000000
VERSION     DAOPKQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45015_length_2621_cov_4.107171"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /gene="tilS"
                     /locus_tag="PK146_06545"
     CDS             complement(<1..542)
                     /gene="tilS"
                     /locus_tag="PK146_06545"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPE91933.1"
                     /translation="MNRERLVKEGWSHEARSLFKSFEIAGRDQGWWDHEGPIVAAVSG
                     GIDSMALLWLLCFRWRGEVIAAHLEHGFRKETALRDAVFVEGICREWDIECHIEHRDI
                     PSLKGRGETLEEAGRRERYAFLDRVASSRGASFIATGHNADDQAETVFLNLLRGTGIR
                     GLRGIPRTREGIVRPVIPDP"
     gene            complement(594..2366)
                     /locus_tag="PK146_06550"
     CDS             complement(594..2366)
                     /locus_tag="PK146_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPE91934.1"
                     /translation="MYRIYTGDPGTVFADPGPDTDLKRVIVRMREEQPERGGNFIEQI
                     IREDLVSGKVSKVMTRFPPEPNGYLHIGHAKSICLNFGLAEQFDGECNLRFDDTNPSK
                     EETEYVESIMEDVAWLGFRWANLCFASDYFDQFHEWALELIRAGKAYVDHQTAEEIRK
                     TRGTLKEPGVGSPYRDRPVEENLELFRKMKEGHFAEGECVLRARIDMAHPNLNMRDPV
                     MYRILHQSHHRTGDKWCVYPMYDFAHGYEDAIEGITHSICTLEFEDHRPLYDWFLDNV
                     SIPSRPRQYEFARLNLTYTVMSKRKLLELVSKQFVSGWDDPRMPTISGLRRRGYTPSS
                     IRSFCKKIGVSRANSLVDVELLHHCIRDELNASAPRAMAVLKPLKLVIENYPEGPGEE
                     IEVVVNPESETPSFRKVPFSRELYIERDDFMEDPPKKYFRLSPGAEVRLKHAYIVKCK
                     GFEKGSDGSVVEVRCEYDPRSRGGEAPDGRRIRGTLHWVSASHAVKAEVRLYDHLFTL
                     RDMDDMEEGTDYKDHLNPRSLEIITGCMVEPYLAKAAPMDRFQFLRQGYFIADPDHTG
                     EKPLFNRTVPLKDSWAKEMSKDPR"
     gene            complement(2483..2559)
                     /locus_tag="PK146_06555"
     tRNA            complement(2483..2559)
                     /locus_tag="PK146_06555"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2523..2525),aa:Met,seq:cat)
BASE COUNT          523 a          781 c          662 g          655 t
ORIGIN      
        1 cagggatcag ggatcactgg acggactatc ccttcccttg ttctgggtat cccccttagc
       61 cccctgatcc ctgtaccccg gagcaggtta agaaagaccg tctcggcctg atcgtcggca
      121 ttatggcccg ttgcgatgaa agacgccccc cgactggaag ccactcggtc aaggaaggcg
      181 tacctctctc ttcttccagc ctcttcaagg gtctcccctc ttcccttcag ggaagggata
      241 tccctatgct caatgtgaca ctcgatatcc cattcacggc aaattccttc cacgaaaacg
      301 gcatccctca gggcggtctc ctttcggaag ccatgttcca gatgggcggc aattacctcc
      361 cccctccacc tgaagcacag gagccacagg agggccattg agtctatacc tcccgagacg
      421 gcggccacga tcggtccttc gtggtcccac cagccctggt ccctgcctgc gatctcgaag
      481 ctcttgaaga gagaccgggc ttcatggctc catccttcct tgacgagacg ctccctgttc
      541 aagatgcctc catttttttc acgaaatgat gccccgttcc cggggacagg ggtctagcgg
      601 ggatccttgc tcatctcctt ggcccaactg tccttgagcg ggaccgtcct gttgaacagg
      661 ggtttttccc ctgtgtgatc gggatcggcg ataaaataac cctgtctcag gaactggaac
      721 ctgtccatgg gagccgcctt tgcaaggtac ggctccacca tgcagccagt gataatctcc
      781 agggatcgag ggttcaggtg atccttgtaa tctgtcccct cttccatatc atccatatcc
      841 ctcagggtga acaggtgatc gtacagtcgg acttccgcct ttaccgcgtg agatgcgctc
      901 acccagtgaa gggtccccct gatcctgcgt ccatctggag cttctccccc cctggatcgg
      961 ggatcgtatt cacatcttac ctcgacgaca ctgccgtcgg accctttctc aaaaccttta
     1021 cattttacaa tataggcatg cttcagtctc acctcggccc ccggtgaaag cctgaagtac
     1081 ttttttgggg ggtcctccat gaaatcgtct ctttcaatgt aaagctccct gctgaatggt
     1141 acctttctga aggaaggggt ttcggattcg gggttgacga ccacctctat ctcctcaccc
     1201 ggaccttcag gatagttctc tatgaccagc ttcaagggct tcagcactgc catggccctg
     1261 ggggcactgg catttagctc atccctgatg cagtgatgaa gtagctccac gtccacaagg
     1321 ctgttggccc tggaaacccc tatcttctta cagaaggagc gtatcgagga aggtgtgtag
     1381 ccccgccttc tcaacccgct gatcgtaggc atccttggat cgtcccatcc tgatacgaac
     1441 tgctttgaga ccagttccaa cagttttctc ttgctcatca ccgtataggt caggttgagc
     1501 ctggcgaact cgtactgcct tggcctggag gggatagaga cgttgtcgag gaaccagtca
     1561 taaaggggac ggtgatcctc gaactccagg gtgcagatcg agtgcgtgat cccctcgatg
     1621 gcatcttcgt agccgtgggc gaagtcatac atggggtata cgcaccattt gtcaccggtc
     1681 ctgtgatgtg actggtgaag gatccggtac ataacaggat ccctcatgtt taggttgggg
     1741 tgggccatat cgatcctggc cctcagaaca cattcacctt cagcaaagtg cccttccttc
     1801 atcttccgga atagctccag gttctcctcc acaggtctgt ccctgtaggg ggatcctact
     1861 ccaggctcct tcagggtgcc tcgtgtcttc cgtatctctt cggctgtctg gtgatcaacg
     1921 taggcctttc ctgcccttat cagttcaaga gcccattcat ggaactggtc gaaataatcc
     1981 gaggcaaaac acaggttggc ccaacggaaa ccgagccagg ccacgtcctc cataatggat
     2041 tcaacgtact ctgtctcttc cttgctgggg ttcgtatcgt cgaacctcag gttgcactcc
     2101 ccgtcgaact gctctgccag tccaaaattg agacagatcg acttggcgtg tcctatgtga
     2161 agatacccgt tgggttccgg tgggaaccgt gtcataactt tgctgacctt tccggatacc
     2221 aggtcttccc ttattatctg ttcgatgaag ttgcctcctc tttccggctg ttcttctctc
     2281 atcctgacga tcaccctctt cagatcggtg tctggtcccg gatcggcgaa aaccgttccc
     2341 ggatccccgg tatagatacg atacactata ataactcaat ttataccggg gatgttccgg
     2401 cccgctcacc cgggtcgcca aagggatcct gaggggtata aaattgcccc cggctggagc
     2461 cgggggcctt attgtctcca agtggtggcg gtgagtggaa tcgaaccact gacactgcgg
     2521 gtatgaaccg catgctctag ccatctgagc tacaccgccg ggaaaattgg ttgcgggggc
     2581 aggatttgaa cctgcgacct tcgggttatg agcccgacga g
//
LOCUS       DAOPKQ010000196         2620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_45043_length_2620_cov_3.397661, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000196 DAOPKQ010000000
VERSION     DAOPKQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45043_length_2620_cov_3.397661"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /gene="der"
                     /locus_tag="PK146_06560"
     CDS             <1..1205
                     /gene="der"
                     /locus_tag="PK146_06560"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPE91935.1"
                     /translation="GEVELGERTFLLVDTGGLLPEGGDSITGNIRRQVLLAVEESDLL
                     ILVVDGKDGMTTLDREVALFLRKRQKPVIVVANKIDDIVHEGREAEAHTLGFKDVIGV
                     SAAHNRNIGELLDRIVSLVPEKGPGQVAPREEEVAVAIVGRPNVGKSTLLNLLSGSER
                     SLVSPMPGTTRDSVDSQVTIGGRHFRFIDTAGLRKKSRIASDVEFYSLVRTHESIGRS
                     DIAVLLMEGEEICTDQDKKMAGLVIEKGKGLIIAVNKWDLLQKDPDLGDRISKKIRDE
                     MPFADFAPLIFISGLTGRGVHKLPEMLSAVQANRSRWLDTSKIGVLVRDVLAFERMPT
                     APGGRMLRIKGCTQVAVNPPAFAFSVNDRDIVTRSFERYVAKRIRDMDDFEGTPLRIF
                     WRSGRKGA"
     gene            1318..1593
                     /locus_tag="PK146_06565"
     CDS             1318..1593
                     /locus_tag="PK146_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPE91936.1"
                     /translation="MTKADLINAVAKSAGITKKQAHEAVGSTFGAIVKSLAKGEKVQL
                     VGFGTFEVRKRAPRKGRNPQDPTKVINIPGKKVPAFRPGKALKDKVR"
     gene            complement(1612..2478)
                     /gene="rsmA"
                     /locus_tag="PK146_06570"
     CDS             complement(1612..2478)
                     /gene="rsmA"
                     /locus_tag="PK146_06570"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPE91937.1"
                     /translation="MPESFLRPNTRIGQNFLVDKNILGVILDSSDVGPGDVVLEVGAG
                     KGVLTEGLLERGASLVHSLEIDRRLEPFLDEIRTRYSNLRILWGDALKGGLPIELDPP
                     PNKMIANIPYHITTPLIWKVLELYSPVGLRYMLLMVQKEAAEKICAPARSKDRYPLGV
                     TIHAMGEARILRRVPPEVFNPSPEVTSALLEITITKGLELPGDKVWRTLIRSAFSHRR
                     KTLLNNLAALLPGTRHEKAALLEKAGLSVSSRAEELELKHWSALLEILSPLIQGEKRQ
                     GTKSPLPANLKP"
BASE COUNT          593 a          678 c          790 g          559 t
ORIGIN      
        1 atggcgaggt ggaactcggg gagagaacgt tcctgctggt tgataccggg ggccttctcc
       61 ccgagggggg agactccatt accggcaaca tcagaaggca ggttcttctg gctgtggaag
      121 agagcgatct cctgatcctt gtggtcgacg ggaaggacgg aatgacgacc ctcgacaggg
      181 aagtggccct gttcctcaga aagaggcaga agccggtcat cgttgttgcc aacaagatcg
      241 atgatattgt ccacgagggc agggaggccg aggcgcatac cctgggattc aaagacgtca
      301 tcggtgtcag cgcggcccac aacaggaaca tcggagagct gctcgaccgg atcgtatccc
      361 tggtcccgga gaagggacca ggccaggttg ccccaaggga agaggaggtt gccgtggcga
      421 tcgtgggtcg tcccaacgtg ggaaaatcca cgctcctcaa ccttctttca ggatccgaaa
      481 ggtctttggt aagcccaatg ccaggcacta caagggattc ggtggattcc caggtcacga
      541 tcgggggcag gcacttccgg ttcatcgata cggccgggtt gagaaaaaag agcaggatcg
      601 cctcggacgt tgaattctac tcactggtaa gaacccatga aagtattgga agatccgaca
      661 ttgcggtcct tctgatggag ggggaggaga tctgcacaga ccaggacaag aagatggctg
      721 gcctggtgat agagaagggc aaggggctga taatcgcggt caacaagtgg gacctcctac
      781 agaaagaccc cgacctgggg gacaggattt caaaaaagat cagggatgag atgcctttcg
      841 ccgattttgc cccgctgatc ttcatttcag ggttgactgg aaggggtgtc cacaaattac
      901 ccgaaatgct ctccgcagtt caggcgaaca ggtcccggtg gctggatacc tccaagatcg
      961 gtgtccttgt ccgggacgtg cttgcatttg aaaggatgcc gacagctccc gggggcagga
     1021 tgctcaggat caaggggtgc acacaggttg cggtgaaccc ccccgccttc gccttttccg
     1081 tcaacgaccg ggacattgtt accagatcct ttgaaaggta cgttgcaaag aggataaggg
     1141 acatggatga tttcgagggg actcccctca ggatcttctg gagatcgggg cggaaggggg
     1201 cctgatctgc actcagcact tgaccacttc tgctgcagat gatattagtg tatctggccg
     1261 tcagtgataa gactaacggc catactgaca tcctgaagag ctggaggtgc gggcaaagtg
     1321 actaaggcgg atctgatcaa cgcggtcgca aaatctgcgg gcatcaccaa gaaacaggcg
     1381 catgaggctg taggttccac ttttggggct atcgtcaagt ctctcgccaa gggggagaag
     1441 gttcagctgg tcggcttcgg gactttcgag gtaaggaaga gagcccccag aaagggaaga
     1501 aatccccagg atcccaccaa ggtcatcaac atcccgggga agaaggtccc cgctttcagg
     1561 cctggcaaag ccctgaaaga caaagttcgc tgacccttcc gatcttattc atcaaggttt
     1621 aaggtttgcg ggcagggggc tctttgtccc ctgccttttt tccccctgga tcaggggaga
     1681 gagtatttct aagagagcag accaatgttt cagttccagt tcttcggccc tggaagatac
     1741 agaaaggcct gccttctcga gcagggcggc cttttcatgc ctggtccccg gcaaaagtgc
     1801 cgcgaggttg ttcaggagag tctttcttct atgggaaaaa gccgagcgga tcagagttcg
     1861 ccataccttg tccccgggaa gttctagacc ctttgttatg gtgatctcga gcagggcgga
     1921 cgtgacctcg ggggaaggat tgaaaacttc cggggggacc ctcctgagga tgcgcgcctc
     1981 ccccatggca tggatagtca cccctagggg gtaacggtcc tttgatcgcg ccggggcaca
     2041 tatcttctcc gcggcctctt tctggaccat cagcagcatg tagcgaaggc cgacagggga
     2101 atagagttcg agcactttcc atataagtgg agtggttata tggtagggga tattggcgat
     2161 catcttgttc ggtggcgggt ccagttcaat gggcaggccg cccttcaggg cgtcccccca
     2221 gagtatcctg agattggagt acctggtcct gatctcatca aggaatggct ccagtcttcg
     2281 atcgatctcc agcgaatgta ccagtgatgc tcccctctcc aggagccctt ccgtgaggac
     2341 tcccttgcct gcaccaactt ccagcacaac gtcaccggga cccacgtcgc tgctgtcgag
     2401 gatcacgcca aggatatttt tgtcgacgag aaagttctgt cctattcggg tgttaggtct
     2461 gagaaagctc tcgggcatta tccatcgacc ccccggtggt tacagatttc ggtatcctgc
     2521 aatgaaaaag cgggaggacc ggaaggtctc ccgctttttg atccctggtc gggacgagag
     2581 gatttgaacc tccgacccct tgacccccag tcaagtgcgc
//
LOCUS       DAOPKQ010000197         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_45220_length_2613_cov_5.195074, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000197 DAOPKQ010000000
VERSION     DAOPKQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45220_length_2613_cov_5.195074"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PK146_06575"
     CDS             complement(<1..821)
                     /locus_tag="PK146_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE91938.1"
                     /translation="MKISMRASKMEPSATLAVVNKAKALKSQGRPVISFGAGEPDFDS
                     PRGAVTYAERAISEGQTHYTQSSGLPELREAVASYYSERFGLDCPKDQIIVGAGAKPL
                     IYETLAALVDPGDEVLIFSPAWVSYVEQIRLLDGREKIVDTTSTGCVPLASRVAEAIT
                     PGTVGMLVNTPNNPTGAVYDEETLRDIAKLAVEHDLWIIFDEIYERLVYGTSTHRNIV
                     SLVPEVMDRTIQVNGVSKAFAMTGWRIGYALAPEWLAPKIGAIQGHLTSNACSIA"
     gene            1020..1562
                     /gene="raiA"
                     /locus_tag="PK146_06580"
     CDS             1020..1562
                     /gene="raiA"
                     /locus_tag="PK146_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPE91939.1"
                     /translation="MDVRFVTRNAELQDELQDYMEKKLVKLEKFFDRIRDTQVEVSFN
                     RGMNVVEITADVNGVIMRGEDYAPDIRKAFDKCLKSIERQIKKHKSFLKDKARMKSAE
                     VSFEIEGFINELSEPEEKKEGIVKTKRFPFRPMSPEEATMQMDLLGHTFFVFRNAENG
                     NVNVVYRRKDGGFGLLIPEE"
     gene            1635..2015
                     /locus_tag="PK146_06585"
     CDS             1635..2015
                     /locus_tag="PK146_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HPE91940.1"
                     /translation="MEPPVPSETFNGGIRLITVHEKDYINGQVNGTSCEKVMTPLARL
                     SRAKIVSLGLFQGDMAKIRSPRGRLVGRVHADEGSSECIFIMDQSVDGANRLTSPTVS
                     PGHGSFCYESSVDIPPLGQEAENA"
     gene            2008..2346
                     /gene="hypA"
                     /locus_tag="PK146_06590"
     CDS             2008..2346
                     /gene="hypA"
                     /locus_tag="PK146_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPE91941.1"
                     /translation="MHELSLVSSVVGSLEELAMGKGWKRITKVTLKIGKMRQVIPEVM
                     VFSFSVASRGTIMEGSELEILEVPVRNRCLSCGENWEGPTFLCPGCGGSEVETESGME
                     MDLESVEVEE"
     gene            2348..>2613
                     /locus_tag="PK146_06595"
     CDS             2348..>2613
                     /locus_tag="PK146_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPE91942.1"
                     /translation="MPRRIVIQQSVMAADEKIAARIRKDLSQRGVLMLNLIGSPGAGK
                     TALLESTLTGAPFRSVVLEGDVATSRDAERIARTGTPVVQINTRG"
BASE COUNT          625 a          657 c          785 g          546 t
ORIGIN      
        1 tgtgctatgg agcaggcatt tgaggtaagg tgtccctgga tagcgcctat tttgggtgcg
       61 agccattctg gggccagcgc gtaaccgatc ctccagccgg tcattgcaaa agccttgctc
      121 acgccgttga cctgtatggt gcgatccatg acctccggga ccagggatac aatgtttcta
      181 tgggtcgagg ttccgtaaac cagcctctcg tagatctcgt cgaagatgat ccagaggtcg
      241 tgttccacgg cgagctttgc tatgtcccga agggtctctt catcgtagac cgccccggta
      301 gggttgttag gggtgttaac gagcatgcca actgttcccg gagttatcgc ctcggcaacc
      361 cttgatgcaa gagggacaca gcccgtcgaa gtggtgtcga caattttctc cctcccgtcc
      421 aggaggcgga tctgctccac gtagctaacc caggcaggcg agaatatcag cacctcgtcg
      481 ccggggtcta caagtgcggc cagggtctca taaattaaag gtttggcacc ggcaccgacg
      541 atgatctggt ctttggggca gtcaagaccg aagcgctccg agtaatagga tgccactgcc
      601 tcacggagtt cggggagccc gctggactgg gtgtagtggg tctgaccctc gctgatcgcc
      661 ctctcggcgt aagttacggc ccctcggggg gagtcgaagt caggttcacc agcaccgaaa
      721 gagatgaccg gtctgccctg gctcttcaag gcctttgcct tgttgaccac ggcaagggtc
      781 gccgaaggtt ccatcttcga tgctctcatt gagatcttca ttctcttcca tctccgttcc
      841 tctgatcttt ttgggagtgt actcctgtca ttataggcct ttacagcgcc gggagaagag
      901 agcatgaagg gtatagtgct aaagggctaa acaggtagac tcattgaatg ggtcttcctt
      961 cgatggtaga atttaagaca gtcatttccc cgaagataaa ggagattgga ggatgagcca
     1021 tggatgtccg gtttgttacc cgaaatgccg aactgcagga tgaacttcag gactatatgg
     1081 aaaagaaact cgtcaaactt gaaaaattct ttgaccgcat ccgagacacc caggtagagg
     1141 taagcttcaa tcggggcatg aacgtggttg agataaccgc tgacgtgaac ggggtgatca
     1201 tgaggggcga ggactacgcc ccggatattc gcaaggcctt cgacaagtgc ctgaaaagta
     1261 tcgagcgcca gatcaagaaa cacaaatctt tcctcaagga caaggccagg atgaagtcag
     1321 ccgaagtctc cttcgagatc gaaggtttca tcaacgagct ttcggagccg gaagagaaga
     1381 aggaaggaat agtcaagacc aagagatttc ccttccgccc gatgtctccc gaggaggcga
     1441 caatgcagat ggaccttctc ggccacacct tctttgtgtt caggaacgct gagaacggca
     1501 atgtgaacgt ggtctacagg agaaaggacg gaggtttcgg actccttatc cctgaagagt
     1561 gatcagggcc ccagataagg aaaagacctg acaaacctac gggtggggtc tctcccgcgg
     1621 ccccacccgt tcgattggag ccccccgtcc caagcgaaac gttcaatgga gggataagac
     1681 tgataacggt ccacgaaaaa gactacatca atggacaggt gaacggtacg tcgtgcgaga
     1741 aggtcatgac acccttggcc cgcctttccc gggcgaagat agtaagcctg ggtcttttcc
     1801 aaggggacat ggcaaagatc cgttctcccc gcgggaggct tgtcggcaga gtgcacgctg
     1861 acgaaggttc gtcagaatgc atcttcataa tggaccagag tgtcgatggc gcgaacagac
     1921 tgacgtcacc caccgtcagc cccggtcacg gatctttctg ttacgaaagt tccgtcgata
     1981 tccctccctt ggggcaggag gcagaaaatg catgaactgt ccctggtgag ttccgtggtc
     2041 ggatccctgg aggagttggc catgggaaag ggctggaaga ggatcacaaa agtcacactg
     2101 aaaataggca agatgaggca ggtcattccc gaggtcatgg tcttttcctt ttcggtggcc
     2161 tcaaggggca cgatcatgga aggctcggaa ctcgagatcc tggaggtgcc tgtaaggaac
     2221 cgttgcctct cctgcgggga gaactgggaa ggtcccacct tcctctgtcc agggtgcggc
     2281 ggaagcgaag tggagaccga aagcgggatg gaaatggatc tggaatcggt ggaggtggag
     2341 gagtaacatg ccccgaagga tagtgataca gcagtcagtg atggccgccg acgaaaagat
     2401 cgcagccagg atcaggaaag acctgtcgca gagaggagtt ctgatgctca atcttatagg
     2461 gtcgccaggc gcaggcaaga cggcgctgct ggagtcgacc ctgacaggag caccattcag
     2521 gtcggtggtt ctggaaggcg atgtggccac atccagggat gccgagagga tcgcccggac
     2581 gggaactccg gtggtccaga tcaacacaag ggg
//
LOCUS       DAOPKQ010000198         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_45236_length_2613_cov_3.111024, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000198 DAOPKQ010000000
VERSION     DAOPKQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45236_length_2613_cov_3.111024"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="PK146_06600"
     CDS             <1..1927
                     /locus_tag="PK146_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPE91943.1"
                     /translation="RTLQGASTITQQLAWNLFLTKEKTLQRKIKEAIISIRLERIYSK
                     EQILEMYLNMIYFGHGAWGIHSASQLYFNKEPSELDLSEASLLAWIVKGPEYYTPLRH
                     PERASERQAYVLGRMAQEGMITPEQAQAARKIDLDFQTLKKPAIMFDEAPYFISHILF
                     EHLLPTYGSDMVYRGGLRVHTTLDLDLQHAAEEAIKGLKSEGAIVALDPGTGEILALV
                     GGKDFEQTKFNRATQAFRQPGSSFKPFVYTAALMKGLRPVDHILDAPVSYDNGIDERG
                     DRDIWEPGNYTGDFKGEVTLLQALVHSYNTVAVRLAHITGIPEIIDVARRFGFTSPHI
                     PNDLSIALGSANVTPLEMASAYSVFANGGSRITPYAIRKINTAAGETLEQYGPNLSPA
                     VPEAIALSMRSVMMEVVRSGTGTRARVPGYEVFGKTGTTNDYTDAWFAGGIPGLVVVV
                     YAGNDDNKTLGRPGTGGVVAAPVWQAFVSKAVKHLTLRESFEIPERAGIDTITVCRDS
                     GFLSAGSCPSTAIFLPSGLAPYSTCPIHGGSWIMAESDRNEPRMLLSPRDEEVLGIPA
                     RMARLYSISISGIAAAPPMEELPTYQPPQMPTYTPPSPPATTPVTPSRQSKPRERTPE
                     EIEDRYQELLKQYGISD"
     gene            complement(1945..>2613)
                     /locus_tag="PK146_06605"
     CDS             complement(1945..>2613)
                     /locus_tag="PK146_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPE91944.1"
                     /translation="SGSAWRAKSRGLEGFSHARQSLRVVDCLEKDGQGLNLSVEVRDG
                     ILKHSKGQVDVREGFSGGEPTLTHEARVVRISDSIAYLNHDLDDAMRAEIVKFGDVPS
                     SVVSRLGSGHGARIGRMVESVVSNSGSDGVNMGSELLGAVEELRSFLYQRVYDSERVL
                     GEEPKVTHVLSTLFEHFEKNPAEDIDCRGYGPALFALDHISGMTDRYAVALFEKMVLP
                     SPWP"
BASE COUNT          589 a          790 c          711 g          523 t
ORIGIN      
        1 caggactctc cagggtgcaa gtacgataac ccagcagctg gcctggaacc tcttccttac
       61 caaagagaag accctccaga gaaagatcaa ggaagcaatt atatcgatcc gtctggagag
      121 gatctactcc aaggaacaga tcctggagat gtacctcaat atgatctact tcggccatgg
      181 ggcctggggg atacattcgg cttcacagct ctacttcaac aaggaaccat ctgaactgga
      241 cctctcggaa gcgtcccttc tcgcatggat agtcaaggga cccgagtact acactccgtt
      301 gcggcacccg gaaagggcct ctgagagaca ggcctatgtt ttgggaagaa tggcccagga
      361 gggaatgatc accccagagc aggcccaggc cgccagaaag atcgatctgg attttcagac
      421 cctgaagaaa ccggccataa tgttcgacga ggccccctac ttcatctccc acatcctctt
      481 tgagcacctc cttccgactt acggaagcga tatggtctac aggggcgggc tcagggtaca
      541 cacgaccctg gacctggacc tgcagcatgc ggccgaagaa gcaataaagg ggctcaagag
      601 cgagggagcc atagttgctc tggaccccgg gacgggcgag atccttgcac tggtgggagg
      661 gaaggacttc gaacagacca agttcaaccg ggccacccag gcctttcgcc agcccgggtc
      721 gtccttcaag ccctttgtct acaccgctgc ccttatgaaa gggctcaggc cggtggacca
      781 cattctggat gctcctgtct cctacgacaa cgggatcgac gagcggggtg atagggatat
      841 ctgggaaccc ggcaactaca ccggggattt caagggagag gtgacattgc tccaggccct
      901 tgtccactcc tacaataccg tcgcggtcag gcttgcccat attactggca taccggaaat
      961 aatcgacgtg gctcgccggt tcggctttac ttcgccccat atacccaacg acctgtccat
     1021 cgctctgggg tcggccaacg tgacccccct ggagatggca tctgcttaca gcgtcttcgc
     1081 caacggcgga tcaaggatca caccctacgc cataagaaag atcaacaccg ctgcaggcga
     1141 gaccctggag cagtacggac cgaacctttc accggctgtc cccgaggcta tcgccctttc
     1201 aatgcgttct gtcatgatgg aggttgtaag gtccggtacg gggaccaggg ccagggttcc
     1261 cggttatgag gtcttcggca agacggggac gaccaacgat tataccgacg catggttcgc
     1321 cggaggtatt ccgggtctcg tggttgtagt atacgccggt aacgacgaca acaagaccct
     1381 gggcagaccg ggtacggggg gtgtagttgc agcccccgtc tggcaggcct ttgtaagcaa
     1441 ggctgtcaaa cacctcaccc tcagggaatc cttcgagatc cctgaaaggg cgggtattga
     1501 cactataact gtctgcaggg actccgggtt cctttcggct ggcagttgtc cttcaacagc
     1561 gatctttctc ccctcgggcc tggccccata ttcgacgtgc cccatccatg ggggttcatg
     1621 gataatggcc gagtccgacc ggaacgaacc gagaatgctg ctctcgccga gggacgagga
     1681 agtcctgggg atccccgccc gcatggcaag gctttacagt atctccatat caggcattgc
     1741 agcagcccct ccaatggagg aacttccaac ctatcagcct cctcagatgc cgacctatac
     1801 tcctccgtct cctcctgcca ccaccccggt cacaccctcc agacagtcca aaccaaggga
     1861 gaggaccccc gaagagatag aggaccggta ccaggagctg ctcaagcagt acggcatctc
     1921 cgattgatcc tgtcggggcc ggcctcaggg ccaggggctg ggcaggacca tcttttcgaa
     1981 aagggcaacg gcgtacctgt ctgtcattcc agagatgtgg tcgagggcga aaagggcagg
     2041 accatatccc cggcagtcga tatcctcggc ggggttcttc tcgaagtgtt caaagagagt
     2101 tgaaaggacg tgcgtcacct tgggttcctc cccaagcacc ctctctgaat cgtagaccct
     2161 ttggtaaagg aaggagcgta gttcctctac ggctccaagg agttctgatc ccatgttcac
     2221 accatcggag ccgctgttgg agacgacgct ctccaccatt ctcccgatcc ttgcgccgtg
     2281 gccggaacca agcctggaaa ctaccgaaga cggaacatct ccaaacttca caatctccgc
     2341 tctcatcgca tcgtcaaggt cgtgattgag ataggcgatg gaatcagaga tacgtacgac
     2401 ccttgcttcg tgtgtcagcg tcggttcacc gccggaaaaa ccttccctga catccacctg
     2461 ccccttggag tgcttcagga taccgtccct gacctctaca gaaaggttca ggccctggcc
     2521 gtctttttcg aggcagtcaa ccactctcag gctctgcctg gcgtgggaga aaccctcaag
     2581 acccctgctt ttcgcacgcc aggcagagcc tga
//
LOCUS       DAOPKQ010000199         2563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_46508_length_2563_cov_3.872010, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000199 DAOPKQ010000000
VERSION     DAOPKQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46508_length_2563_cov_3.872010"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PK146_06610"
     CDS             complement(<1..1275)
                     /locus_tag="PK146_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE91945.1"
                     /translation="MSAKDIILALRSRGADPGFIVFTGQGDERLAVEMMRLGAFDYLL
                     KGLDMLDLLPRVLERLFLQRDTQRRLADAINSLRESEERFKALHNASFGGIAIHDKGI
                     ILDCNLGLSEISGYSLEELRGMDGLLLIAERSRDLVMNNILSGYEKPYEAFGLRKNGE
                     EYPLRLEARAVPFRGKTVRSVEFRDITGEKLAEKKIRESERMYRRLTENMKDVIWVID
                     PYELRFSYVSPSVKDLRGYTAEEVMASPLEEAFTTAGSKHLLERVKTGMDGFLAGRIT
                     SNDYFTEEIEQPCRDGSTVWTEAITRFVADGESGKVEIHGVTRDITARRKAEREYQTL
                     FEEMVEGFALHEIICDEKGVPVDYRFLAVNPAFESMTGLRADEITGKTVLEVLPGTEK
                     YWIKTYGRVALTGEPAIFDNYSREMDKHFSVTA"
     gene            complement(1277..1537)
                     /locus_tag="PK146_06615"
     CDS             complement(1277..1537)
                     /locus_tag="PK146_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE91946.1"
                     /translation="MAMHIEEKSGRVLVLEDDEGLRGLILKRLRKEGYKAEGASSGAE
                     ALELAAADPEMVLLLDHLLPDMSGSAAANRSRNGPAAGSPAP"
     gene            complement(1537..1989)
                     /locus_tag="PK146_06620"
     CDS             complement(1537..1989)
                     /locus_tag="PK146_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE91947.1"
                     /translation="MAMNEEVVILIAEDDEGHAGLIRKNLFRGGIVNETIHFTDGQAV
                     LDFLFRKGAGQIREAGRAYVLLLDIRMPSVDGIEVLSRVKTDPELRKIPVVMITTTDD
                     PGEISRCHQLGCSSYISKPVDYEAFVEAIRQLGLFLSVVQIPELNGGK"
     gene            complement(1976..>2563)
                     /locus_tag="PK146_06625"
     CDS             complement(1976..>2563)
                     /locus_tag="PK146_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPE91948.1"
                     /translation="AASLNYIRSGTLQMDSLLKGLLKLSRAGRVGLDIVPLDMNEIVS
                     GIVSAMAFQINEADVEVDLGDLPPCLGDPVQVGSVFSNILGNALKYLDSQRRGKISVR
                     GWVEKNMSLYRVFDNGVGIPSDQQERIFGLFVRLDRSGTEGDGIGLTLARNVLGRMNG
                     EIRVESVPGKGSSFYVTLPSSGNGAEPQGRFNGDE"
BASE COUNT          532 a          793 c          635 g          603 t
ORIGIN      
        1 cgcggtcacg ctgaaatgct tgtccatttc tcgtgaatag ttgtcgaaaa tggcgggttc
       61 tcctgtcagg gcaaccctgc cgtatgtctt gatccagtac ttctcggtcc ctggaagaac
      121 ctcgagaacg gtctttcctg ttatctcatc cgccctgagc ccggtcatgc tctcgaaggc
      181 agggttgacg gcaaggaacc tgtaatccac gggcacgcct ttttcgtcgc agattatctc
      241 gtgaagggca aaaccctcta ccatctcctc aaagagggtc tggtactccc gctcggcttt
      301 tctcctggcg gtgatgtccc ttgtaacccc gtggatctcc accttacccg actccccgtc
      361 ggccacgaaa cgggttatcg cctctgtcca tactgtggaa ccgtccctgc atggctgctc
      421 aatttcctcg gtaaaataat cgttagatgt gatcctgcca gcaagaaaac catccattcc
      481 ggtttttacc cgctccaata aatgcttgct tcctgccgtg gtaaaggcct cttccagggg
      541 actggccatg acctcttctg ccgtataccc cctcaggtcc ttgaccgagg ggctgacgta
      601 gctgaacctc aattcatagg gatcaataac ccagatgacg tctttcatgt tctccgtgag
      661 tctccggtac atccgctcgc tttcgcgaat ttttttctcg gcaagcttct ccccggttat
      721 atcgcgaaat tccaccgacc ggactgtctt tcccctgaag ggaacagccc tggcctccag
      781 cctcagtgga tactcctccc cgttcttgcg gagcccgaag gcctcgtagg gcttctcgta
      841 acccgaaagt atattgttca tgaccagatc cctggacctt tcggctatca gaagaaggcc
      901 gtccatcccc ctgagttctt ccagggagta tcccgatatc tccgaaagac ccaggttgca
      961 gtcgaggatg atccccttgt cgtggattgc aatgcccccg aaggaggcat tgtgaagagc
     1021 cttgaagcgt tcctcgctct ccctgaggga gtttatcgca tcggcgagac gcctctgggt
     1081 atccctctga aggaaaagcc tttccagaac cctggggagc aggtcgagca tatccagccc
     1141 cttgaggagg tagtcgaagg cccccagcct catcatctca acggccagcc tctcgtcccc
     1201 ctggccggtg aacacgatga atcccggatc ggcaccccgg ctccggaggg ccaggataat
     1261 gtcctttgcc gacatgtcag ggagcaggtg atccagcagc aggaccattt cgggatctgt
     1321 tggccgccgc agatcccgac atgtcaggga gcaggtgatc cagcagcagg accatttcgg
     1381 gatctgcggc ggctaactcc agggcttcgg ctccggagga agcgccttct gccttgtacc
     1441 cttccttccg gagtctcttc aggatcaggc ccctgagccc ttcatcatcc tcgagaacaa
     1501 gtacacgacc cgatttttct tcgatatgca tcgccatcat ttacctccgt tcagttccgg
     1561 tatctgaaca acagaaagga aaaggcccag ttgccggatg gcctcgacaa aggcctcgta
     1621 gtccacgggc tttgagatgt aactgctgca accaagctga tgacagcggg agatctcccc
     1681 ggggtcatcg gtggtggtta tcatcaccac cgggatcttc ctgagttccg gatcggtctt
     1741 gactctggag agcacctcta tcccgtctac ggatggcatg cggatgtcaa gaagcagcac
     1801 gtaggccctg cctgcctccc tgatctgacc ggctcccttt ctgaaaagaa agtccagcac
     1861 agcctgtccg tcggtgaagt ggatcgtttc gttgactatc ccgccccgga agaggttctt
     1921 tctgatcagg ccggcatgac cctcatcatc ttccgcgata aggatgacca cttcttcatt
     1981 catcgccatt gaacctcccc tggggttccg ccccgttccc cgaggaagga agtgtcacat
     2041 agaaactgct gcccttcccc ggcacggact ccactcttat ctccccgttc atcctgccca
     2101 gaacgttcct tgcaagggtg agtcctatgc catccccttc ggtccccgac cggtccagcc
     2161 ggacaaaaag cccgaatatt ctctcctgct ggtccgaagg aatccccaca ccattgtcga
     2221 aaacacggta aagagacatg tttttttcga cccatccccg gacggagatc ttgccccttc
     2281 tctgagaatc aaggtattta agggcattgc ccagaatatt cgaaaaaacg gaaccaacct
     2341 ggacagggtc acccaggcag ggagggaggt ccccgagatc cacttccacg tcggcctcgt
     2401 tgatctggaa agccattgca gaaactatac ccgatactat ctcgttcata tcaagaggta
     2461 ctatgtcgag accgaccctg ccggccctgg acaacttcag caaccccttg aggagggagt
     2521 ccatctgaag tgtgccgctt cttatgtagt tgaggcttgc ggc
//
LOCUS       DAOPKQ010000200         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_46684_length_2556_cov_4.155138, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000200 DAOPKQ010000000
VERSION     DAOPKQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46684_length_2556_cov_4.155138"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..490)
                     /locus_tag="PK146_06630"
     CDS             complement(65..490)
                     /locus_tag="PK146_06630"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPE91949.1"
                     /translation="MSKGETAMDALLNFIKALADIQRVRIIYALSEGELCLCSLQEIL
                     ELSPSTVSRHVSVLKQAGLVVSRREGKWRYFRLAPGLEKTPGGDLVKWLVRNLEDDHK
                     AGIQAEKVALLREQQNERCAPCSDGLKKRQKRNGRGQVG"
     gene            complement(593..1804)
                     /locus_tag="PK146_06635"
     CDS             complement(593..1804)
                     /locus_tag="PK146_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPE91950.1"
                     /translation="MKFSKRITSMQSSPIRRLVPYAEAAKAEGKKVYHLNIGQPDIVT
                     PPGFLESIKKFAGDVIAYSASHGSYELIDAMIDYYKGFDINLERKNLIVTNGGSEALS
                     FALMATCDPGDEVLVPEPFYANYNAFASSVNVKLVPITTRAREGFHLPPKAEILKKIT
                     PLTRAILFSNPGNPTGVIYTREEMEMIADIALEKDIYVIADEVYREFVYDGLKYTSFG
                     NIKRLEDRVIIVDSISKRYSACGARIGCILSRNEELMAQTMKLAQGRLCVPTLEQVGA
                     VALYKTPKSYLEEVNKEYQARRDILISKVREMPGVICEEPKGAFYVVAKLPVDDAEKF
                     VIWMLKDFHVDNETVMMAPAEGFYATPGLGRDEARLAYVLKKEDLSRAMDILKAGLEA
                     YPGRIKSASNF"
     gene            1946..2302
                     /locus_tag="PK146_06640"
     CDS             1946..2302
                     /locus_tag="PK146_06640"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPE91951.1"
                     /translation="MPLMSLKQFRHNAEEIAGSLPTELFSRLNGGIIVEPSIREDGDY
                     VIMGEYLEDPGLGNLIILYYGSFREMLGSAPFEEWNEEIRETMIHELRHHVESLAGVD
                     DLSVEEDQVLREESSA"
     gene            complement(2224..>2556)
                     /locus_tag="PK146_06645"
     CDS             complement(2224..>2556)
                     /locus_tag="PK146_06645"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPE91952.1"
                     /translation="RNARAFLDLAEREGSFSTWLWDFADGRPIVGGWDTQEQIPARTP
                     LSQHVSRELKSRGFGFMGPVVVYSHMQAVGLVNDHLRDCFRYAELSSRRTWSSSTERS
                     STPARDST"
BASE COUNT          559 a          690 c          696 g          611 t
ORIGIN      
        1 catagggtaa ggtaacgctc gaaaacgttg gtcatctttc gttcgctgtt gttgaatttc
       61 tggctcatcc aacctgtccc cttccattcc tcttctgtct ctttttcagt ccgtcactgc
      121 agggagcgca tctttcattc tgctgttccc tcaagagggc taccttttca gcctggatcc
      181 cggctttgtg atcgtcctca aggtttctga caagccattt gacaagatca ccccccggtg
      241 ttttctccag tccgggggca agtctgaaat accgccattt gccttcccta cgggatacta
      301 ccaggccggc ctgttttaaa accgatacat gccttgagac agtcgaaggg gacagctcga
      361 gtatttcctg gagactgcag aggcacagtt ccccttccga cagggcatag atgatcctta
      421 ccctctgaat atccgccagt gccttgatga agttcagaag agcatccatc gccgtttctc
      481 ctttgctcat ttggccaaac aacgaaatgt taccagacca gccgttcaag gtcaagaaaa
      541 aaccgcccct ggaaggggcg gctaaagcgt tcttctgacc ggttcactaa agctagaagt
      601 tggaagcgga cttgatcctt cccgggtaag cctcgaggcc ggccttgagg atgtccatgg
      661 ccctggacag gtcctccttc ttgaggacgt atgctagccg ggcctcatcc cttccgaggc
      721 cgggcgtagc gtaaaagccc tcggccgggg ccatcatgac tgtctcgttg tcgacgtgaa
      781 agtccttcag catccagatg acgaactttt cggcatcgtc gacggggagc ttggccacca
      841 cgtaaaaggc acctttgggc tcttcacaga taactccggg catctccctg accttggaga
      901 tgaggatgtc ccttcgcgcc tggtactcct tgttgacctc ctcaagatag gacttggggg
      961 tcttgtagag tgccacggcg cccacctgct ccagggttgg tacgcagagc ctcccctggg
     1021 caagcttcat ggtctgggcc atgagctctt cgttcctgga gaggatgcaa cctatcctgg
     1081 ccccgcaggc gctgtagcgc ttggagatgg agtcgacgat gatgaccctg tcctcgagcc
     1141 tcttgatgtt gccgaagctg gtgtatttga gaccgtcgta gacgaactcc ctgtagacct
     1201 cgtcggcaat gacgtagata tccttctcaa gggctatgtc ggcgatcatc tccatctctt
     1261 ccctggtgta gatgactccc gtggggttac cagggttgga gaagagaatg gcccttgtaa
     1321 ggggggtgat cttcttcagt atctccgcct tgggaggcag gtggaaaccc tcccttgccc
     1381 tggtggtaat ggggaccagc tttacgttga ccgaggaggc aaaggcgttg tagttggcgt
     1441 agaagggttc ggggacaaga acctcgtcac cggggtcaca ggtggccatg agggcaaagg
     1501 acagagcctc gctgccaccg ttggtgacga taaggttctt tctctccagg ttaatatcga
     1561 aacccttgta gtagtctatc atggcatcaa tgagctcgta actgccgtgg gatgccgaat
     1621 aggcgatcac gtccccggca aatttcttga tggactccag gaaaccaggc ggggttacta
     1681 tgtcgggctg gccgatgttg aggtggtaaa cctttttgcc ctccgccttt gcagcctcgg
     1741 cataggggac aagcctgcgg atcggcgaag attgcatcga ggttatcctc ttggagaatt
     1801 tcattaaaaa cctacctccc tgtaggaatg atatttacca aatgttattt tagcacaatt
     1861 tttcacatac ataatacact tgtcacgccc cgccagtcag ctgcaaaatt atggcaggga
     1921 gaaattctaa ttgggaggtc cttagatgcc gctcatgagc cttaaacaat tcagacacaa
     1981 cgccgaagag atagccggat cgctgccgac cgaacttttc agtcgtctga acggaggaat
     2041 aatagtcgaa ccctcgatcc gggaggacgg cgactacgtc atcatggggg agtacctgga
     2101 agatcctgga ctgggaaacc tcatcatcct ctactacggg tccttccggg aaatgctcgg
     2161 gagtgctccc tttgaagagt ggaacgaaga gatcagggag acaatgatac acgaactcag
     2221 acatcacgta gagtctctcg caggagtgga cgacctctca gtagaggagg accaagtcct
     2281 tcgggaggaa agttcggcgt agcggaagca gtcccggaga tggtcattta ccagtccgac
     2341 cgcctgcatg tgggagtaga ccaccacggg tcccatgaag ccgaaccccc ttgacttgag
     2401 ctccctgctt acatgttgcg acaagggggt tctggcgggt atctgttcct gggtatccca
     2461 acctccgaca atgggtctgc cgtcagcgaa atcccatagc caggttgaga atgaaccctc
     2521 tcgttccgcc aggtcgagga aggcacgggc attcct
//
LOCUS       DAOPKQ010000201         2543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_46991_length_2543_cov_5.584003, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000201 DAOPKQ010000000
VERSION     DAOPKQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46991_length_2543_cov_5.584003"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="PK146_06650"
     CDS             <1..1127
                     /locus_tag="PK146_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA activase"
                     /protein_id="HPE91953.1"
                     /translation="VIGDLISFLEREAREKGQERVCSEYLVFMPTTSGPCRFGKYAEV
                     LALSLDSLGFGSIPIVSPTTEKGYLDPKILGITWPLMTRTGFFRDIYNAIRAADLFDD
                     LVLRFRPYSFDRGSFNKTARERLSLLEETFRREGASLKGIVEWGRRTAEVFSSLCPES
                     HERSPLVLYLGEIYMRQHDPYTGQVVELLEREGLEMVRGPVTEWLRYVIHIASRKSPG
                     LELKAAALYMNFAEKMIERGMNDILGDRKVLPHPEDTLPEVEREGIYHSDITGESSIS
                     IGLFNAFLSGRLKEDPENPVCGVFHVGPFTCMQEGVATARIKGMLKARRRVDPGLVAP
                     VMHASFGESPNPNLEAEVAAFRDQCLIMRRSLKGSLAKAL"
     gene            complement(1131..1796)
                     /locus_tag="PK146_06655"
     CDS             complement(1131..1796)
                     /locus_tag="PK146_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91954.1"
                     /translation="MSKEAYPLVLVIMASVAAVLLLVRGPGQIGRKMLRLMGLVFPLV
                     VFPLWGIGVYRTCVILPAAVLALLEAARFSGRRDRSTLGRLLSPIAKEGEGRGFFGFS
                     TYFWGSALASLAPGGTGPAVTAMATLCDPWASMAGTAWGGQGSSLGKTWVGSLTCLLV
                     SSMTGLSFAVLFPRAGISLQQTLLASVAMTAAERYSPGEFDNLSIQVVGAAVVTLSSF
                     GSL"
     gene            1900..2436
                     /locus_tag="PK146_06660"
     CDS             1900..2436
                     /locus_tag="PK146_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPE91955.1"
                     /translation="MSFLDLARERRSVRSYRTDPVARDAIDRCLEAARLAPSACNSQP
                     WRFVVVDTPEFLEKFVEAAYSGIHTMNAFARTAPVQIAVVRENARTAARLGGFIRGVD
                     FTLIDIGIACEHLVLQAAEEGIGSCILGWFNERSVRKILGLPRSARVDLLICMGYAKT
                     PGPALKHRKTLEDIRSYL"
BASE COUNT          584 a          732 c          769 g          458 t
ORIGIN      
        1 gtgtgatcgg tgatctgata tccttcctgg agcgggaggc cagagagaag ggtcaggaga
       61 gggtatgcag cgagtatctc gtcttcatgc ccaccacgag cggaccctgc cggttcggga
      121 agtacgcaga agtgctggcc ctgagtctgg acagcctggg attcgggagc attcccattg
      181 tctcgcctac gacagagaag ggctacctcg acccgaagat cctcggcata acctggcctt
      241 taatgacaag gaccggtttt ttccgcgaca tctacaacgc cataagggcc gcggacctct
      301 tcgacgacct ggttctccgc ttccgcccct actctttcga caggggatcc ttcaacaaga
      361 cagcccggga aaggctaagt cttcttgaag agaccttccg ccgggaaggg gcctctctca
      421 agggcattgt tgagtggggg aggagaacag ctgaggtctt cagctctctc tgccctgaaa
      481 gccatgaaag atccccactg gtcctctacc tgggcgagat ctacatgaga cagcacgatc
      541 cttacactgg gcaagtagtc gagctgctcg agagggaggg gctggagatg gtccggggac
      601 cggtcaccga gtggctgcgg tatgtcatcc atatagcatc aaggaagagt cccggactgg
      661 agttgaaggc cgcggccctg tacatgaact tcgccgagaa aatgatagaa aggggcatga
      721 acgacatcct cggcgacagg aaggtgctgc ctcatcccga ggacaccctt ccggaagtgg
      781 agcgcgaggg gatctatcac tccgatataa ccggagaatc atcaatatcc atcggactgt
      841 tcaacgcctt cctctcggga aggctcaaag aggatccgga gaaccccgta tgcggggtct
      901 tccacgtggg gcccttcacg tgcatgcagg aaggggtcgc cactgcaagg ataaagggaa
      961 tgctcaaggc gagaaggagg gtcgaccctg gacttgtcgc accggtcatg cacgcctctt
     1021 tcggggagtc ccccaacccg aacctggagg ccgaagtggc cgccttcagg gaccagtgcc
     1081 ttatcatgag gaggtctttg aaagggtccc tggcaaaagc cctctaggcc tcagagagag
     1141 ccaaaggaag agagggtgac aaccgctgcc ccgacaacct gtatcgagag gttgtcgaac
     1201 tcccccgggg agtacctttc tgccgctgtc atcgccaccg aggccagcaa tgtctgctgc
     1261 agcgagatgc cagcccgggg gaagaggacg gcgaaggaaa gaccggtcat cgatgatacc
     1321 agcaggcagg tcaaactgcc gacccaggtc tttcccagag aggatccttg tcctccccag
     1381 gcggtaccgg ccatggatgc ccagggatcg cagagtgtgg ccatggccgt cacggcagga
     1441 cctgttccgc ccggtgcaag ggatgcaagt gccgatcccc agaaatatgt ggagaacccg
     1501 aaaaaacctc tcccctcccc ctcctttgct attggtgaca ggagtcggcc cagcgtcgac
     1561 ctgtcacggc gaccggagaa cctggccgct tcaaggaggg ccagaacggc agccggaagg
     1621 ataacacagg tcctgtaaac gccgatcccc cacaacggga agacaaccag gggaaagact
     1681 aggcccatta acctgagcat cttgcggcct atctgtccgg ggcccctgac aaggaggagg
     1741 accgccgcta cgcttgccat tatcaccagg accaaggggt acgcctcttt ggacattgtt
     1801 caaatacctc cttgcctgaa ggtggcgcca gatggtacaa gggaagccca ggggcattag
     1861 agcgaagacg gactgcccga cagggcgtgg aggtgagcta tgtctttcct tgatctggca
     1921 agggaacgaa gaagcgtaag atcctacagg accgacccgg tcgccaggga tgccatagac
     1981 aggtgtcttg aggctgccag gctggccccg tcggcctgca actcgcagcc atggcgcttt
     2041 gttgtggttg acactccgga gtttctggag aagttcgtcg aggccgccta ttcggggatc
     2101 cacaccatga acgccttcgc caggacagcc cccgtccaga tagctgtcgt tcgcgagaac
     2161 gccagaacgg ccgcccgttt gggcggtttc atcaggggcg tggacttcac cctgatagat
     2221 atcggtatag cctgcgaaca cctcgtcctc caggcggcgg aagagggaat aggaagctgc
     2281 atcctcgggt ggttcaacga aagatccgta aggaagatac tcgggctgcc ccgcagcgca
     2341 agggtcgacc tcctgatatg tatggggtac gcaaagacgc caggccccgc cctgaaacac
     2401 agaaagaccc tggaagatat tcggagctac ctttgaacca acctgctctc cccttgcaca
     2461 aggtcggcac agacgaaagc ttccgattcc tccccaaggg cggaacgggc catccttccg
     2521 atatggaagg acgcagcagt atg
//
LOCUS       DAOPKQ010000202         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_47210_length_2535_cov_3.413710, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000202 DAOPKQ010000000
VERSION     DAOPKQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47210_length_2535_cov_3.413710"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PK146_06665"
     CDS             complement(<1..814)
                     /locus_tag="PK146_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPE91956.1"
                     /translation="MKRLWSTAVAVAFVLITAGVSFSAVQPVVVGVDVTGNQEVVSEH
                     ILGVVGTKAGDQLSSEQIQQDIDTIYGLGFFSYVDVGVVEQFGGVFVEFRVQENPVVQ
                     DIRFSGNTVFTADELMEVVFTRPGSVFNRVFFRHDLQRIGEKYEKAGYVLVRVEDVGI
                     RDGIVDVRILEPKIGDIVIQGNKKTKTQVIRREFKLQPGDLFNATVMRHSLNKLNQMG
                     FFEDVSVGLEPTEDPSAVNIILTVEEGKTARVGLTIGHGSESGWTGGAMYEEL"
     gene            complement(914..1486)
                     /locus_tag="PK146_06670"
     CDS             complement(914..1486)
                     /locus_tag="PK146_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91957.1"
                     /translation="MEKNLKRVHRWIDRCIVACKSGKWDSALAEVECARAELESASEE
                     IWERASGAGNQYPVVRMVGLSFRSMAVAILVIMMAAIPISTGGYAPLGVADTRQLHLE
                     WVTADEQGVLSALRTSLSEMNLARDIPHQTSPPETEPRLGPPAMERVAPQIAREENGA
                     PAVALDEILTLIEIGQRALRGEGIIIRSPE"
     gene            complement(1596..2189)
                     /locus_tag="PK146_06675"
     CDS             complement(1596..2189)
                     /locus_tag="PK146_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPE91958.1"
                     /translation="MKDDGQDNPKREFLSREEEAALWGLCGEGDEEAREKLILAYRPL
                     VFWIARKFRVTPQRYQDLVQEGMLALIKAVDRFEPSRNIRFVTYGFYRIKGQMTNFLQ
                     RVESRAPCPVDDSEVLIPDPFDVDCLDWKICLDDGIEALSAREGEVVRALVLEGCKAR
                     DYAESIGMDVSNVYRIQRQAMAKLKAWLELEDATDRP"
     gene            complement(2249..>2535)
                     /locus_tag="PK146_06680"
     CDS             complement(2249..>2535)
                     /locus_tag="PK146_06680"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPE91959.1"
                     /translation="GESFSDLVSRVGRGLEEILSRDGDTLLVVCHGGTIRAALSFLAG
                     IPSTSAWKFRMDNCSITAVDMTPDRTTLRYVNDAIHTRVGPDLAVRFPLL"
BASE COUNT          553 a          770 c          638 g          574 t
ORIGIN      
        1 tcagttcctc gtacatggcc cctccggtcc atcccgattc ggacccgtgg cctatggtaa
       61 gacccacacg agctgtcttt ccttcttcaa ctgtgagaat gatgtttacc gctgatggat
      121 cttccgtagg ttcaagacca acacttacat cttcgaagaa tcccatctgg ttcaatttat
      181 tcagagaatg tctcattaca gttgcattaa aaagatctcc tggctgtagc ttgaactctc
      241 ttcttatgac ttgggtcttg gtctttttgt tgccctggat aacaatatct ccaattttgg
      301 gctcgagaat acgtacatca actatgccat cccggatacc cacatcctca acccgaacaa
      361 gaacgtagcc tgccttttcg tacttctctc ctatcctctg gaggtcgtgc ctgaagaaga
      421 cccggttgaa gaccgatccc gggcgtgtaa agacaacctc catgagttca tcagctgtaa
      481 agaccgtgtt accggaaaag cggatgtcct gtacaacggg gttctcctgg accctgaact
      541 cgacgaagac cccgccgaac tgctctacca caccgacatc aacgtaggag aagaagccaa
      601 gaccgtagat cgtgtcaata tcctgctgga tctgttcgga actcaactgg tccccggcct
      661 ttgtgccgac cacaccgagg atatgttcgg aaacaacctc ctggttcccg gtaacgtcga
      721 cgccgacgac gaccggctga acggccgaaa atgatacgcc ggccgtaata agaacgaacg
      781 ctacggcgac agccgtggac cataaccgtt tcactcgaac agcacccctt tccctgaact
      841 ggtctctccc gaggggcagt gaccccacgg ctcaattctc ttcggtcgta tcacaccatg
      901 gaattataaa ctattattcg ggacttcgga taattattcc ttcacccctt aaggctctct
      961 gtcctatctc gatgagggtc aggatctcgt caagggctac ggcaggggca ccgttctcct
     1021 cccttgcgat ctggggcgcc actctctcca ttgcaggagg tcccaaccgc ggttctgtct
     1081 ccggcggaga cgtctgatgg gggatatccc gtgccaggtt catttcactc agactcgttc
     1141 tcagtgctga aagaacgccc tgttcgtcgg cggtgaccca ctccaggtgc agctgcctgg
     1201 tgtcggccac cccgagaggt gcgtatcctc ccgtggatat gggaatggcg gccatcatga
     1261 tgaccaggat cgccacggcc atggacctga aggagagtcc aaccatacgt accaccgggt
     1321 actggttacc cgccccggag gctctctccc agatctcctc gctggccgac tcgagttctg
     1381 ccctggcaca ctcgacttcg gccagggcgg agtcccattt tccgctcttg caggctacaa
     1441 tgcacctgtc gatccatcga tgcacgcgtt tcaggttctt ctccatctct tatctgcctc
     1501 cgagaccgca ctggcaatat gcccccgaaa agggactatg ccccgaaaag ggactatgcc
     1561 ccgaaaaggg actatgccct tttattatca cagtatcagg gccgatcagt ggcatcctcc
     1621 agttcaagcc aggccttcag ttttgccatc gcctggcgct ggatcctgta gacgttgctt
     1681 acatccatac ctatgctttc agcgtaatct ctcgccttgc aaccctcaag cacaagggcc
     1741 ctgacgacct ctccctcccg ggcggagagc gcttcaatgc catcgtcaag acatatcttc
     1801 cagtccaggc agtccacatc gaagggatca gggatcagga cctcggagtc gtcaacaggg
     1861 catggggcac gggactccac tcgctgaagg aaattggtca tctgtccctt gatcctgtag
     1921 aaaccgtagg tgacgaaccg gatgttccgg gagggttcga acctgtcaac agcctttata
     1981 agggcaagca taccctcctg gacaagatcc tggtagcgct ggggggtgac ccggaatttg
     2041 cgggcgatcc agaagacgag agggcggtag gccagtatca gcttttcgcg ggcttcttcg
     2101 tcgccttccc cgcaaaggcc ccacaaagcg gcctcttcct ccctggaaag gaattccctt
     2161 ttagggttgt cctgtccatc gtccttcatc ggctacctcc ccggactcta ctggaccctt
     2221 tataccacga cgggaaggca tttgaccact acagcaaggg aaatctgacg gcaaggtcag
     2281 gtcccacgcg ggtatggatg gcatcgttga cgtaacgtag ggtcgtacgg tccggcgtca
     2341 tgtccactgc ggtaatggaa cagttgtcca tcctgaactt ccaggccgat gtggagggga
     2401 tgcctgccag aaaggacaga gcggcacgga tcgtaccccc gtgacagacc acgagaagag
     2461 tgtccccgtc gcgggaaagg atctcctcca gcccccgtcc cactctggaa acgagatcgg
     2521 agaacgactc tcctc
//
LOCUS       DAOPKQ010000203         2528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_47393_length_2528_cov_4.237768, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000203 DAOPKQ010000000
VERSION     DAOPKQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47393_length_2528_cov_4.237768"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1035
                     /locus_tag="PK146_06685"
     CDS             55..1035
                     /locus_tag="PK146_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPE91960.1"
                     /translation="MKEQFLSPKIFSQPEMVPTRFGYGDGLVELGKNNPSVVVLGADL
                     SSSLCVDRFRNAYPERFIQTGIAEQDMMALAAGLALSGKVPFVSTYGVFCTGRAWDQL
                     RTTVAYARLNVKIGSGHGGVSVGADGATHQALEDISITRTIPNVTVIVPADYHETFKA
                     TVAAASISGPVVIRFGREKVPVITTDRTPFTVGKAQIFKNGTDVTIAACGQMVYESLL
                     AAEALAKDGISARVINCHTIKPIDSKTLIRAAEETGAVVTAEEHQIMGGFGSAVAEVL
                     SQNCPVPIRMIGVQDTFGQSGDPDDLMEEYCLRAGSIVKAAVEAVGMKKG"
     gene            1043..2239
                     /locus_tag="PK146_06690"
     CDS             1043..2239
                     /locus_tag="PK146_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE91961.1"
                     /translation="MSVPFAERTKRLGTETAFAVGAEAASLAATGVKVYPFHLGDLNI
                     PTPEHIVEAANRAIREGKTSYCPTPGITPLREAMAEEIYRTRGFKIDADNVSIQTGGK
                     PVIGKFIMTLMNPGDEVLYPNPGFPIYESMINFHEGVGKPYGFRETDKGFSLDLEAIE
                     RQISPRTKLIIYNNYHNPTSAESDMEEMEHLADICLKHNIYVLSDEAYFDILYDGTPK
                     SITRFQGLPERTVILYTFSKKFAMTGWRLGAAIGPREVIEAISRFNVNDESCTNNFVQ
                     WAGVAALRGPLDDHRKMIDILRERRDTALSILNNTKGIHVATPDSTFYLFPNVTKAME
                     KLGMEEVEDFRKFVLEKTGVSFTTRNHFGTPLPGETQKYIRLAYSGIDTPEIIEGLGK
                     MKELLG"
BASE COUNT          648 a          691 c          709 g          480 t
ORIGIN      
        1 ttcaccggag caggccaagg cggcacttca tgagctttca cgggaggtca tccgatgaaa
       61 gaacaatttc tttctccgaa gatcttttcc caacctgaaa tggtccccac ccgtttcgga
      121 tacggtgacg gactggttga actggggaag aacaacccct cagtggtcgt ccttggagct
      181 gatctcagct cttctctctg cgtagaccgc ttcaggaacg cttatcccga acggttcatc
      241 cagaccggga tcgccgagca ggacatgatg gccctggcgg cggggctggc tctctccggc
      301 aaggtgccct tcgtaagcac ctacggggta ttctgcacag gaagagcctg ggaccagctg
      361 aggactacag tggcatatgc caggctgaac gtaaagatcg ggtccggaca cggcggggtc
      421 tccgttggag ccgacggcgc aacccatcag gcccttgaag acatctccat taccaggacc
      481 ataccgaacg ttacggtgat agtgccagcg gactatcatg agaccttcaa ggcaaccgtt
      541 gcggcggcat ctatctccgg cccggtggtt atccggttcg gcagggagaa ggtgcctgtg
      601 ataaccacag acaggacgcc ctttaccgtg ggtaaagccc aaattttcaa aaacggcact
      661 gacgtcacga tcgcggcctg tggacagatg gtctacgaat cgcttttggc ggctgaagca
      721 cttgcgaaag atgggatatc tgcaagggtg atcaactgcc ataccattaa accgatcgat
      781 tcaaaaaccc ttataagggc agccgaagaa acgggagctg ttgtgacagc agaggaacac
      841 cagataatgg gcggcttcgg aagcgccgtt gcggaagttc tcagccagaa ttgccctgtg
      901 ccgataagga tgatcggggt ccaggacacc ttcggacaat ccggtgatcc cgacgacctg
      961 atggaagagt actgcctcag ggcaggatcc attgtgaaag ccgctgttga agctgtagga
     1021 atgaagaaag ggtgatctct ccatgtcagt tcccttcgct gaaagaacaa aacgactagg
     1081 aacagagacg gcttttgccg ttggtgccga agcggccagt ctggctgcca caggggtgaa
     1141 ggtttacccc tttcacctgg gagatcttaa catccctacc ccagaacaca tcgtggaggc
     1201 agccaaccgg gcaatcaggg aaggcaagac gagctactgt cccacccctg gtattacgcc
     1261 cctgagagag gctatggccg aggagatcta caggaccagg gggttcaaaa tcgacgccga
     1321 caacgtgagc atccagaccg gcgggaaacc ggtgatcggc aaattcatca tgaccctcat
     1381 gaaccccggc gatgaagtac tctaccccaa cccggggttc cccatctatg aatcgatgat
     1441 caacttccac gaaggtgtag gaaaacctta cggcttcagg gagactgaca agggtttctc
     1501 actggatctg gaagcgatag aacgtcagat ctcccctcgg acaaagctca tcatctacaa
     1561 caactaccat aacccgacca gcgccgagtc tgacatggaa gagatggaac acctggccga
     1621 tatctgcctt aaacacaaca tttacgtgct tagcgacgag gcatacttcg acattctcta
     1681 tgacggtacc cccaagagta ttacccgatt ccaggggctt cccgagagga cggtcatcct
     1741 ctacaccttc tccaagaagt tcgccatgac gggttggcgc cttggagcgg cgatcggtcc
     1801 cagggaggtg atcgaggcca tctcccgttt caacgtgaac gacgaatcct gcacaaataa
     1861 cttcgtccag tgggcaggcg ttgccgcctt gagaggacca ctggacgatc accgtaaaat
     1921 gatcgatatc ctcagggaaa gacgcgacac agccttatcc atccttaaca acacgaaagg
     1981 tatccacgtc gctacccccg acagcacttt ctacctcttc ccgaacgtta cgaaggctat
     2041 ggagaagttg ggaatggaag aggtcgagga cttcaggaag ttcgtcctgg aaaaaactgg
     2101 cgtctcgttc accaccagga accacttcgg cacacccttg ccgggcgaga cccagaagta
     2161 cattcgactg gcctactcgg ggatcgacac ccccgagatc atcgaaggac tcgggaagat
     2221 gaaggaactg ctggggtagg agcgaaacgc gtaaacggtc aacgcgaagt cccgcggtct
     2281 ctgtggaggt aaacagtgaa cagaaggccg aagagcaagg cggcataccc tcgaggcaag
     2341 ctgaaggact tggtcccgac aaggtcccga gacgaattaa cgagggagga gttttaacga
     2401 ggggcctccg gccttcggcc tacggggcgc aacccaggct gaaggcaaca ggaagggcag
     2461 gatcaagggc gcaactctta actcgtgact tatgactggg aaaacaggag caggatcttg
     2521 tcattgcg
//
LOCUS       DAOPKQ010000204         2523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_47517_length_2523_cov_4.345219, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000204 DAOPKQ010000000
VERSION     DAOPKQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47517_length_2523_cov_4.345219"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK146_06695"
     CDS             <1..714
                     /locus_tag="PK146_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPE91962.1"
                     /translation="DVINKGITQKEMEDTFRKVFSRGWDRVKLYFMMGLPTETLDDIE
                     AIMEISLLARDMGRRLGKRPRIGVSVAGFVPKPHTPFQWEAQATVEDLAMKGGTLKRM
                     AKKAGIGLNYHEPAQTFLEGVMARGDRRIAAVIERAWRNGARFDGWTECFDMGRWMNA
                     FDSEGVDPKEYTGRTRGVEEVFPWEVVDVGVSRAFLWSERERALSGDLTPDCRGGSCN
                     LCGWQQDGCPVLEEKNGQG"
     gene            701..1321
                     /locus_tag="PK146_06700"
     CDS             701..1321
                     /locus_tag="PK146_06700"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPE91963.1"
                     /translation="MARVRMIFSKRGRVCFVPHVEMPALFCRALRRVGARIEMTEGMS
                     PHPRISLGPALPVGVPALGEPLEVWIGNWEEQFGGSVNSFLPRGVTVLRYGVVDGRQR
                     LSEECTAALYRIIFREGQAFEIIRKMISGGWVPVGHTLDISASGNWIDVAVSSPGQTG
                     GGTIVKSLVKEGVVGSWSELLVTRLAVGRWSGKSVSPLPCGGGGRG"
     gene            1318..>2523
                     /locus_tag="PK146_06705"
     CDS             1318..>2523
                     /locus_tag="PK146_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPE91964.1"
                     /translation="MICDRKIFANVIDPEETRVAIVEGGRLNEIFVERMWDRQRSGEI
                     YKARVDSVIPGMNAAFLNLGEGRNAFLYLEDARGTEVRQNLDLLVQVTKTARKGKGAR
                     VTTRISLPGRFLVLVPGGHETGVSRRVSEEQRERLRGYARAIRPDGFGIIVRTAAEGV
                     DQDILEEDLENLLHIWDGISRMAREQDAPCLLYQDLGLVGRILRDEPFGAVDEIIVDS
                     EEEYRNVLAFLERFPSNGSTPEVNLHRGNIPILEFYGIEKDLESALERKVWLGSGAYL
                     VIDQTEALTVIDVNTGKFTGDGDPRSTILRTNLESAREVARQLRLRALGGIVVVDFID
                     MEDEEDQRELLDTLRELFRNDRSRARVFGVTPLGLVELTRKRARSDIRASFTRGCPFC
                     GGTGVVPREE"
BASE COUNT          531 a          650 c          894 g          448 t
ORIGIN      
        1 gatgtgatca acaagggtat cacccagaag gagatggagg acaccttccg gaaggtcttc
       61 tccaggggct gggacagggt caagctttac ttcatgatgg ggttgccgac ggagaccctg
      121 gatgatatcg aggccataat ggagatctct ctcctggcca gggacatggg gcgcagactg
      181 ggcaagaggc ccaggatcgg ggtctccgtg gcggggttcg tccccaagcc gcacactccc
      241 ttccagtggg aggcccaggc aaccgtggag gatctggcca tgaagggcgg cactctcaag
      301 agaatggcca aaaaggccgg cataggcctt aactaccacg aaccggccca gacttttctg
      361 gagggggtca tggccagggg ggaccggagg atagctgcag tgattgagag ggcatggagg
      421 aacggtgccc gcttcgacgg atggaccgag tgtttcgaca tgggaagatg gatgaacgcc
      481 ttcgattctg aaggggtaga cccgaaggag tacacaggca ggaccagggg ggtcgaagag
      541 gtcttcccgt gggaggtcgt ggacgtggga gtatcccgcg ccttcctgtg gtctgagagg
      601 gagagagccc tgtcgggaga tctcactccg gactgcaggg gtggcagttg caacctctgc
      661 ggttggcagc aagatggctg ccccgtcctg gaggagaaaa atggccaggg ttaggatgat
      721 cttctccaag agagggaggg tctgcttcgt acctcacgtg gagatgcccg ccctgttctg
      781 ccgggccctc aggagggtag gggccaggat agagatgacc gaggggatga gccctcaccc
      841 caggataagc ctcggaccgg cccttccggt gggcgtccct gccctggggg agcctctgga
      901 ggtttggatc gggaactggg aggagcagtt cgggggatct gtcaacagct ttctgccccg
      961 gggcgtgacc gtcctgaggt atggtgtcgt cgacggaagg cagcggctga gcgaagagtg
     1021 cacggcagcc ctctacagga tcattttcag ggagggtcag gcctttgaaa tcatccggaa
     1081 aatgatctcc ggggggtggg tgcctgtcgg ccatactctg gacatttcgg cctccgggaa
     1141 ctggatcgat gtggcggtct cttctcccgg tcagaccggg ggtggcacga tcgtcaaaag
     1201 ccttgttaaa gagggcgtgg tcggctcctg gagcgaactg ctggtaacaa ggcttgccgt
     1261 gggcagatgg tcagggaaga gtgtaagccc tttgccctgc ggaggaggtg gtcgggggtg
     1321 atctgcgaca ggaagatctt cgcaaatgtc atagaccccg aggagacgag ggtcgccatc
     1381 gtagagggag gccgcctcaa cgagatattc gtggaacgta tgtgggaccg ccagcggagc
     1441 ggagagatct acaaggccag ggtcgacagc gtaatccccg gcatgaacgc tgcgttcctg
     1501 aacctcgggg aggggcgcaa cgccttcctg taccttgagg acgccagggg aacggaggtc
     1561 aggcagaacc tggacttgct tgtccaggtg accaagaccg ccaggaaagg caagggggcc
     1621 agggtcacaa ccaggatatc cctgcccgga agattcctgg tccttgtccc gggaggtcac
     1681 gaaacggggg tctcgaggcg cgtatcggag gaacagagag agcggttgcg ggggtacgcc
     1741 cgggcaataa ggcccgatgg gttcgggatc atagtcagga ccgcggctga gggcgttgac
     1801 caggatatcc ttgaagagga cctggagaac ctcctccata tctgggacgg catctcccgc
     1861 atggcccggg agcaggatgc cccctgcctt ctctaccagg acctgggact ggtggggagg
     1921 atcctcaggg atgagccctt tggtgccgtg gacgagataa tcgtcgatag cgaggaggag
     1981 taccggaacg tcctggcctt cctcgaacga ttcccatcca acggttccac ccccgaggta
     2041 aaccttcaca ggggcaacat acccatcctc gagttctacg gtatagaaaa ggacctggag
     2101 tcggccctgg aaagaaaggt atggctgggg tccggggcct acctcgtcat tgaccagacc
     2161 gaggccctta ccgtgatcga cgttaatacc ggcaaattca cgggcgatgg agatccgagg
     2221 agcaccattc tgaggaccaa cctggagtcg gcccgggaag tggccaggca gctgaggctg
     2281 agggcgctgg gtgggatagt ggtcgtggat tttatcgata tggaggacga ggaggaccag
     2341 agagagctcc tcgacaccct cagggaactg ttcaggaacg accgctcaag ggcgagggtc
     2401 ttcggcgtga cacccctggg gctggtggag ctcacaagga agagagctcg ctccgacatc
     2461 agggcctcct tcaccagggg atgtcccttc tgcggaggca cgggcgtggt cccgagggag
     2521 gag
//
LOCUS       DAOPKQ010000205         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_48625_length_2484_cov_3.649650, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000205 DAOPKQ010000000
VERSION     DAOPKQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48625_length_2484_cov_3.649650"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1539)
                     /gene="argH"
                     /locus_tag="PK146_06710"
     CDS             complement(130..1539)
                     /gene="argH"
                     /locus_tag="PK146_06710"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPE91965.1"
                     /translation="MWHGRFREDTAEIVQHFTQSLDLDWRLAPYDVYGSIAHVRMLEH
                     VGLLDQREAAVIEDGLRQVLSDIQEGRFTPRESMEDVHMNIEARLTEIIGPLGEKLHT
                     GRSRNDQSATAVRLYLRKELYSVGDGLITLLDALIDRATVHRHVIVPGYTHLQQAQPI
                     SMGQYWLAHFWALARDFRRVLFALESVNECPLGSGALAGSTLPLDRGFTSAMLGFDRT
                     SDNSMDSVAHRDHLLDCQYAMAVLMVHTSRMAEDLVIYNSREFGWVILPDAFCTGSSM
                     MPQKKNPDVLELIRGRAGRVIGDLVDLLTIIKGLPMTYNRDMQEDKRALWSSIQITRD
                     VLEVLPQLIARVEIDEEKAAASFGDGFALATDVAEYLVMKGMPFRSAHEKVGNLVKWC
                     LDRKRKLTSLGPEEWREIIPEVEEDLFPLLNIESSVSRRNTYGGTSFKQVALQIERGM
                     ETLEVFRRKLAAYPARFGL"
     gene            complement(1565..>2484)
                     /locus_tag="PK146_06715"
     CDS             complement(1565..>2484)
                     /locus_tag="PK146_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HPE91966.1"
                     /translation="RAGLKFGAIDCGSNSVRCLAVLHRDGILEYVDSGAWITRLTEGI
                     ESGEYRIAPKAVERTAVAITEAKDLLERSGVGPERTVFFATESLRSAANGAFAVAELE
                     RAAGLALRVLGGEEEAALGRRGALLGTRGAGCVFDLGGGSLEIGWDGSSLSIPAGAVR
                     MKARFEEDREKTGEEILNLLAGKGIPSPEEVVGTGGTSSSIVMMLQGVPVTDYHPARI
                     HGYLVSAAELDELAEKLVNAPVEDRMRIVGLDPKRADIIVAGIFVIRTLLDLFRVSGY
                     THSETDLLWGVCAEKAAAEGLPVFSAKIG"
BASE COUNT          455 a          829 c          638 g          562 t
ORIGIN      
        1 tgtctccttg cctttcggct cttgcctttc ctgcctccag catcctgttt cctgtctcct
       61 gtttcctgtc tcctgtctcc tgtttcctgt ctcctgtttc ctgtctcctg tctccttgcc
      121 ttaccgcccc tacaacccaa accttgccgg gtaggcagcc aacttcctgc ggaagacctc
      181 cagggtctcc attccgcgtt caatctggag agcaacctgc ttgaacgatg tccccccgta
      241 ggtgttcctg cgcgacacgg aactctctat gttgaggagg gggaacaggt cctcttccac
      301 ctcggggatg atctctcgcc actcttccgg cccgaggctt gtcagttttc tcttcctgtc
      361 gagacaccat ttgaccagat tccccacctt ctcgtgggcg ctgcggaagg gcataccctt
      421 catcacgagg tactccgcca catccgtcgc aagggcaaac ccgtcgccaa aggaagccgc
      481 ggccttctcc tcgtcgatct ccactcgggc tatcaactgg gggagcactt ccagaacatc
      541 cctggttatc tgtatcgacg accagagggc ccgcttgtct tcctgcatat cccggttgta
      601 ggtcatcggg agccccttga ttattgtaag gaggtccaca agatccccta tgactcttcc
      661 cgcacgtccc cttatgagtt ccagaacatc ggggttcttc ttctggggca tcatgcttga
      721 gcctgtacag aaagcgtcgg ggaggatgac ccagccaaac tcgcggctgt tgtagatcac
      781 aagatcctcc gccatcctgc tggtatggac catcagcacg gccatggcgt actggcagtc
      841 caggaggtgg tccctgtggg cgacggaatc catgctgttg tcgctggtcc tgtcgaaacc
      901 gagcattgcg gaggtgaacc cccggtccag gggaagcgtg gaacccgcga gggccccgct
      961 tcccagtggg cactcgttga cggactccag ggcaaagagg acccttctga aatcccttgc
     1021 cagcgcccag aagtgtgcca gccagtactg tcccatgctg atgggctgag cctgctgcag
     1081 atgggtgtag ccgggcacta tgacatgcct gtgcaccgtc gccctgtcta taagggcatc
     1141 aaggagagtt atcaggccgt cgcccacgga atagagttcc ttcctcaggt aaagcctcac
     1201 cgccgtggcc gactggtcgt tacggctcct gccggtatgc agtttctctc ccagcgggcc
     1261 gatgatctcc gtaagacgtg cctctatgtt catatggacg tcctccatcg actccctggg
     1321 ggtaaagcgc ccctcctgga tatcggaaag gacctgcctc aggccgtcct cgatgaccgc
     1381 tgcctcccgc tgatccagga ggcccacatg ctcgagcatc ctgacgtggg caatactccc
     1441 gtagacatcg tagggagcaa gcctccagtc gagatccaga gattgcgtga agtgctgaac
     1501 tatctccgcc gtgtcttccc ggaatcgtcc atgccacata aagatctacc tcccggatac
     1561 tgttctatcc aatctttgcg gaaaagacgg gcaacccttc cgcggcggcc ttttcggcgc
     1621 atacccccca cagaagatcc gtctcgctgt gggtgtagcc cgataccctg aaaagatcaa
     1681 gcagggtcct tattacgaag atccccgcga cgataatatc tgccctcttc gggtccagcc
     1741 cgacgatacg catcctgtcc tccaccgggg cattcacgag tttttcggca agttcgtcca
     1801 gttctgccgc cgaaaccagg tacccgtgga tccttgccgg atggtagtcc gtgaccggca
     1861 ccccctgaag catcataacg atggaagaag aagtcccgcc cgtcccgacg acctcctccg
     1921 gtgagggaat ccccttccct gcaagaaggt tcaggatctc ttctcctgtt ttttctctgt
     1981 cctcttcgaa ccgggccttc atcctcacgg ctcccgccgg gatcgacagg gacgagccgt
     2041 cccatccgat ctccaggctt cctccgccga gatcaaagac gcaaccggct ccccgggtcc
     2101 caaggagagc gcctcttctg ccgagagctg cctcctcctc tcctccgagg acccttaggg
     2161 caagtccagc tgcgcgctcc agttccgcga ccgcgaaagc gccattggcc gcgcttctga
     2221 ggctctccgt cgcgaaaaag accgttcttt ccggccccac tcccgatctt tcgagaagat
     2281 cctttgcctc ggtaatcgcg acggcggtcc tctcgacggc cttcggagca atcctgtatt
     2341 cgccggattc tatgccttcg gtaagcctgg ttatccaggc ccccgaatcg acatattcca
     2401 ggatcccgtc cctatgaagt acggccaggc agcggaccga gttggaaccg cagtctatgg
     2461 ctccaaactt caggcctgcc cgca
//
LOCUS       DAOPKQ010000206         2479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_48760_length_2479_cov_5.175743, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000206 DAOPKQ010000000
VERSION     DAOPKQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48760_length_2479_cov_5.175743"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="PK146_06720"
     CDS             <1..1433
                     /locus_tag="PK146_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE91967.1"
                     /translation="VGYLSLSRRADTLSSGESQRIRLATQIGSKLSGVLYVLDEPTIG
                     LHPRDTTRLLGTLEAIRDLDNTVLVVEHDRDTMTRADYIVELGPGAGEHGGMITACGK
                     PSEIVSGNSLSGPFISGQDSGTSFRRGELPDAPGWIVVKGAEENNLKSIDVSIPLGRL
                     VCISGVSGSGKSTLLNEVLYKGLKRKLDKNFRGRAGQHRDIEGWEALRNVVMIDQTPI
                     GRTPRSNPATYTQLFTPIRELFASLPESRIRGYKAGRFSFNVRGGRCEACKGDGEVRI
                     SMLFMPDVYVKCEVCHGKRYNRETLDIRYKDKNIAEVLEMSVDEARAFFGDIPRISRR
                     LSVLQQAGLGYIRLGQPATTLSGGEAQRVKLAEELGKQFRGHTLYILDEPTTGLHYLD
                     VRKLLVLLQKIIDQGNTVLLIEHNLDVLASADHIIDLGPEGGHQGGTVVATGSPDEIV
                     RNAKGHTARHLGEFLDSIKRREADVS"
     gene            1423..2223
                     /gene="rlmB"
                     /locus_tag="PK146_06725"
     CDS             1423..2223
                     /gene="rlmB"
                     /locus_tag="PK146_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPE91968.1"
                     /translation="MSVNPTGKKDPGSEDLVFGKNAVKAVLDSLPEHCSVVLVSERMA
                     TNEREQFLETCRRAGVRWQLVPPSRIDRICPGDRHQGIAARMLPVALTDLESFLKGLD
                     PAGGPSLIVLLDHVQDPQNLGAIARSAEIFGADGMVIPRRRSVLPTSTVMRTSSGAIA
                     RVPVVGVVNVVRTLEDLKGNGFWVVGLDHRKGSVLWECDLPERMVLVVGSEDRGMSRL
                     TSETCDLLTRVPMKGRTGSLNASTAAAIGMYEWMRSFTAGCVVQSVKE"
     gene            2402..2476
                     /locus_tag="PK146_06730"
     tRNA            2402..2476
                     /locus_tag="PK146_06730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2434..2436,aa:Val,seq:gac)
BASE COUNT          565 a          645 c          757 g          512 t
ORIGIN      
        1 gggtgggata cctatctctc tcaagacggg ctgataccct gagctctggt gagagccagc
       61 gcataaggct ggcaacccag ataggctcca agctgagcgg tgtcctttac gtccttgatg
      121 agccgaccat aggacttcat ccgagggaca ccacaaggct tctgggaact cttgaagcga
      181 tacgggacct ggacaacacc gttcttgtcg tcgaacacga ccgggacaca atgacaaggg
      241 ccgattacat cgttgagctg gggcccggcg ccggagagca tggcggcatg atcacagcct
      301 gtgggaaacc ctccgagata gtctcaggga actcactttc cggtcccttc atatcgggac
      361 aggattctgg tacttcattt cgcagggggg aactgcccga tgctccgggt tggatagtgg
      421 tgaaaggagc tgaagagaac aacctgaaga gcattgatgt ttcgatcccc cttggccgac
      481 tggtttgcat aagcggtgtc tcagggtccg gcaagagcac ccttcttaac gaagtgctct
      541 acaagggtct gaagcgaaaa ctggacaaaa atttcagggg aagggccggg cagcacaggg
      601 atatcgaagg gtgggaagcc ctgaggaacg tggtaatgat agatcagaca cccatcggca
      661 ggacgcctcg ctccaatccg gcgacctata cccagctttt tactcccata agagagctct
      721 ttgcctctct tccagaatca aggatcaggg gctacaaggc ggggcgcttc agtttcaatg
      781 tccggggagg tcgctgtgaa gcctgcaagg gagacggcga agtacggatc tccatgctat
      841 ttatgcccga tgtctacgtc aagtgcgagg tctgccacgg aaagaggtat aaccgggaaa
      901 ccctcgatat aaggtacaag gacaaaaaca tcgccgaggt tcttgagatg tccgtagacg
      961 aagccagggc ctttttcggg gatataccca ggatatcgag gaggctttcg gtgcttcagc
     1021 aggcaggact tggatacatt cgcctcggac agcccgcgac gactctcagc ggaggggagg
     1081 cccagagagt aaagctggca gaggaactgg ggaagcagtt ccgggggcac acgctttaca
     1141 tactggatga acctactaca ggccttcact acctggatgt aaggaaactg ctggtccttc
     1201 tgcagaagat catcgaccag ggcaacacgg tactgcttat tgaacacaac ctggacgtac
     1261 ttgcctctgc cgatcatatt atcgacctcg gtcccgaggg aggtcaccag ggagggaccg
     1321 tcgtggccac gggatctccc gacgagattg ttcggaatgc gaaaggtcat accgcccgtc
     1381 acctgggaga gttcctggat agtatcaaaa ggagggaagc cgatgtcagt taatccgaca
     1441 gggaaaaaag acccgggcag cgaggacctg gtgttcggga agaacgccgt caaggctgtt
     1501 ctggactcgc ttcccgaaca ctgctccgtg gtccttgtct cggagaggat ggcaaccaat
     1561 gaaagggagc aattcctgga aacttgcagg agggccggtg taaggtggca acttgtccct
     1621 ccctccagga tcgacaggat ctgcccgggt gatcgacacc agggaattgc tgccaggatg
     1681 ctacccgtgg ccttgactga cctcgagagt tttctgaaag ggctagaccc ggccggtggc
     1741 ccctccctga tcgttctgct cgatcacgtc caggatcccc agaacctggg agccatagcc
     1801 agatcggcag agatcttcgg cgccgatggg atggtcattc ccagaaggcg ttccgttctc
     1861 cccacatcga cggtgatgag aacgagttca ggggcaatag cgagggtccc cgtggtaggc
     1921 gttgtgaatg ttgtcaggac actcgaagac cttaagggta acgggttctg ggtagtgggg
     1981 ctggaccacc gtaaagggag tgtcctctgg gagtgcgatc tgcccgaaag aatggtcctc
     2041 gtcgtaggga gcgaggaccg agggatgtca aggctcacct ccgaaacgtg cgacctcctt
     2101 acaagggtcc caatgaaagg gcgcaccggc tcgctgaacg cttcgacggc ggctgcgatc
     2161 ggaatgtacg agtggatgag gtcctttacg gccgggtgcg ttgtgcagtc tgtgaaggag
     2221 tagcgcacac cggttcatat gttcccggtg atgacctttg ctcgggtggt ttccgttcct
     2281 tggctatttc ttttcgggag gtcctcccgg gtgtattcgg tcggccttgc gaactatccc
     2341 cctgaccctc accagctgtt gttgacaagc tatcgggcca cgggtaaaat tcaacacgct
     2401 tgggtggtta gttcagagga agaacgcttc cttgacgcgg aagaggtcgc aggttcgagt
     2461 cctgcaccac ccaccattt
//
LOCUS       DAOPKQ010000207         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_48915_length_2474_cov_5.451840, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000207 DAOPKQ010000000
VERSION     DAOPKQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48915_length_2474_cov_5.451840"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="PK146_06735"
     CDS             <1..1374
                     /locus_tag="PK146_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPE91969.1"
                     /translation="ADLDAATRRVMQMEIEEAALAREADKASMERLETLRKELQDARA
                     SADAMRARYDAEKQAIGKLQGIREEIESTKREIERAERDYDLNRAAELKHGRLPDLER
                     RLAAEEELLVTKQGGDKLLREEVTAEEISRIVSKWTGIPVSKMLEGEREKLLRLDDIL
                     HRRVIGQDEAVRLVADAVLRARSGIKDPRRPVGSFIFLGPTGVGKTELARTLAEALFD
                     SEENMIRIDMSEYMEKHSVSRLVGAPPGYIGYDEGGQLTEAVRRKPYSVILFDEIEKA
                     HHDVFNILLQILDDGRLTDSHGRTVNFTNTIIIMTSNIGAADLMEGITSSGEIPDAVR
                     ESVMKQMRASFRPEFLNRVDDIVLFKPLRIGEIEQIVDLLAAGLQRRLRDQGMDLEIT
                     PAARNLIAREGYDPVYGARPLKRFISREVETPIARLLVSQGLTEGSRIVVEDENGKIV
                     TRNTAKP"
     gene            complement(1534..>2474)
                     /locus_tag="PK146_06740"
     CDS             complement(1534..>2474)
                     /locus_tag="PK146_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE91970.1"
                     /translation="EVIVRYSPDWSVTFANDAYCRYYQTTPEEILGSPMTTHIPEKYR
                     KKTLERLANLTPVSPTLISEEETILPSGEIRWQQWFDTALFDSEGDIVEYQSVGRDIT
                     ERKQMEESLRVSSDRLERVLDETVRVLAGTMRIRDVYTADHQEGVARLARAMGEEMGL
                     SDDVLKGLWMAALIHDIGKLHIPGEILNKPTTLTALEYEIIKRHTEVGAETLRGISFP
                     WPIAHIISQHHERMDGSGYPRGLKGRQILPEVRILSVADVMEAMCSHRPFRPAYPVEV
                     ALNTIRSGAGTLFEEKAVEACLKVFGNGFEFVRQGT"
BASE COUNT          553 a          685 c          733 g          503 t
ORIGIN      
        1 gccgacctgg atgccgccac gcgaagggta atgcagatgg agatcgagga ggccgccctt
       61 gccagagagg ccgacaaggc aagcatggaa aggcttgaga cgctgagaaa agagctgcag
      121 gacgcccggg cgtcggctga tgcaatgagg gcccgatacg atgctgaaaa acaggccata
      181 gggaagctgc agggaatccg cgaggagatc gaaagcacaa agagggagat agagagagcc
      241 gaaagggact acgacctcaa cagggctgca gaactaaagc atggaagact gcctgacctt
      301 gagagaaggc ttgctgctga agaggaactc ctggtcacga aacagggggg cgacaagcta
      361 ctcagggaag aagtcaccgc cgaagagatc tccaggatcg tctcgaaatg gaccggcatc
      421 cccgtatcga aaatgctgga aggcgagagg gaaaagcttc tgcgtctgga cgacatccta
      481 caccggcggg tgatcgggca ggacgaagcg gtccgtcttg tagccgacgc agtgctcagg
      541 gcccggtcgg ggataaaaga tcccagaagg cccgtgggct ccttcatatt ccttggccca
      601 actggtgtgg gaaagacaga acttgcccgc acccttgccg aggcactctt cgactccgag
      661 gagaacatga tccggataga tatgtcggaa tacatggaga aacactccgt gtcccgactt
      721 gtcggcgctc ctccaggcta catagggtac gatgaaggcg gacagctgac cgaggcagtg
      781 aggagaaaac cctattcggt gatccttttc gacgagatcg agaaggccca ccacgacgtg
      841 ttcaacatcc tgctccagat actcgacgac ggaagactga ccgatagtca cgggcgaacg
      901 gtgaacttca ccaacaccat aatcattatg accagcaaca ttggggctgc ggacctcatg
      961 gagggcatta cttcctccgg cgagataccc gatgccgtcc gtgaaagcgt aatgaagcag
     1021 atgagggctt ccttcaggcc tgaattcctg aatcgcgtgg atgacatagt actcttcaaa
     1081 cctctgagga taggggagat cgagcagata gtcgaccttc tggccgctgg tctccagagg
     1141 cggctcaggg accaggggat ggatctggag ataactcccg cggcaaggaa cctcatcgcc
     1201 agggaggggt acgacccggt atacggagcc aggccgctaa agcgtttcat atccagggag
     1261 gtcgagaccc ccatagctcg cctgcttgtc tcccaggggc tgacagaggg ctcaagaatc
     1321 gtggtcgaag atgagaatgg gaagatcgta actcgtaaca ctgcaaaacc ataaaggcgt
     1381 gaacggcatc ctctgacggc cttggacttg gttctccatt cacgatctat gattcacgat
     1441 ctacgactca cggttcatgt cctgagtact tgaggctttc ctgaagcctg aagtctgtcg
     1501 ccacgctcca gggtctctaa cttttcacgc accttaggtt ccctgcctca cgaactcgaa
     1561 cccgttcccg aataccttca ggcaggcctc tacggccttc tcttcgaaga gtgtccccgc
     1621 cccggagcgg atagtgttca gggcgacctc gacggggtag gcaggacgga aaggtctgtg
     1681 ggagcacatg gcctccatca cgtcggcgac ggagaggatc cttacctccg gaagtatctg
     1741 tctgcccttc agacccctgg ggtagcccga gccgtccatt ctctcgtggt gctgggagat
     1801 gatatgggca atgggccagg ggaaggagat ccctctcagt gtttcggctc ccacctcggt
     1861 gtgccttttg atgatctcat actcgagggc cgtcagcgtt gtcggtttgt tgaggatctc
     1921 cccgggtatg tgaagcttcc ctatgtcgtg gatcagggcg gccatccaca gaccctttaa
     1981 cacgtcatcg gaaagaccca tctcctcccc catggctcgg gccagtcggg ctacgccctc
     2041 ctggtggtca gctgtataga catcccggat cctcatggtc ccagccagga ccctcaccgt
     2101 ttcgtcgagc actctttcga gcctgtcgct gctcaccctg agggactcct ccatctgctt
     2161 tctttccgtt atatcccgtc ccacggactg atactcgacg atgtcccctt cgctgtcgaa
     2221 gagggccgtg tcgaaccact gctgccagcg gatctcccct gagggaagga tcgtctcctc
     2281 ttccgagatc agggtggggg ataccggtgt caggtttgcc agtctttcca gggttttctt
     2341 cctgtacttc tcggggatat gggtcgtcat gggggatccg agtatctctt cgggcgttgt
     2401 ctggtagtat ctgcagtagg cgtcgttggc gaatgtcacg gaccagtcgg gggagtagcg
     2461 cacgataact tcct
//
LOCUS       DAOPKQ010000208         2443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_49793_length_2443_cov_4.692630, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000208 DAOPKQ010000000
VERSION     DAOPKQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49793_length_2443_cov_4.692630"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PK146_06745"
     CDS             <1..324
                     /locus_tag="PK146_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPE91971.1"
                     /translation="YILHPTFGYLLGFIAGAWTIGFISERSGEPSFALYLTACLIGTA
                     VIYTVGAFVLYLNLNHVAGKETSIRQVLEFGVAPFIAGDIFKMLGTAALASRVGSRLR
                     QISRR"
     gene            436..1311
                     /locus_tag="PK146_06750"
     CDS             436..1311
                     /locus_tag="PK146_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPE91972.1"
                     /translation="MDQEGVPGKQKIIILMFVLYVVWGSVFLANRFSLESFPPFMLNA
                     VRFLSAGIFLYVLLRVKGEEGLDLRGWLESFVVGAMLFIGGAGLLCVGQQWVASGLSA
                     TLLATTPLWTVLFAGIWEKMPTTMEWAGLGIGIAGVALLNLDRGISGNPLGAGIILVA
                     SIIWSLGSVLNNRFQGLGKPIASATEMIAGGLLLLILSLLVGERISWPLTARATMGMF
                     HLVVFSSIIGFTLYRFMLRTVRPALATSYTLVNPITATVLGALFAGESVSLVSIAAMA
                     VVLTGVFLVFRARAI"
     gene            1327..2052
                     /locus_tag="PK146_06755"
     CDS             1327..2052
                     /locus_tag="PK146_06755"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPE91973.1"
                     /translation="MSLDLVIESVSAGDLSVLTGAGMSTASGLPDFRGKDGLWKQKDP
                     RLLASLSAMENDRAEFVNFYRYRIRMFEGVKPNRGHHILAEWEARGLLKGVITQNVDG
                     LHQEAGSREVHELHGNLRRVRCSRCRAAYESSLFLERDDCPACGGSLRPGVVLFGEML
                     PEGPMDAAEELSGTASSFLVLGSSLEVSPANAYPMLAKRAGAKLFIINMTPTQLDYMA
                     DGVIHGDIVETLEKIDKSVTHGS"
     gene            complement(2195..>2443)
                     /locus_tag="PK146_06760"
     CDS             complement(2195..>2443)
                     /locus_tag="PK146_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPE91974.1"
                     /translation="VLPGTMRRMTLEIARDAGFPVEERCPLRDELPLCAEFFITGSIK
                     EILPVVRIGKTAVGGGKPGPVSAHLRRLYLQNLERWRE"
BASE COUNT          488 a          684 c          714 g          557 t
ORIGIN      
        1 tacattctcc atcccacctt cggctatctg ctgggcttta tcgccggggc ctggaccatc
       61 ggtttcatat ccgaaaggtc cggggagccc tccttcgccc tgtacctgac ggcgtgcctc
      121 attgggaccg ccgtgatcta caccgttggg gcatttgtcc tttatctgaa tcttaaccac
      181 gttgccggca aggagacctc catcaggcag gtactggaat tcggggtggc acccttcatt
      241 gcaggtgata ttttcaagat gctgggaaca gctgcccttg cttccagagt tggctcaaga
      301 ttgaggcaga tctccagaag ataggctcaa cgcagcggaa ggccatcggt ccccatcggg
      361 ggtgataaac agtgccactg gcgctacaat aatatgcaaa ttaaaacttt tcgtcttctc
      421 ccggggggct ttgaagtgga ccaggaaggt gttccaggaa aacagaagat catcatccta
      481 atgttcgtac tctatgttgt atggggttcc gtctttctcg ccaaccgttt ctcccttgag
      541 agttttcccc ccttcatgct gaacgcagta aggttcctct ccgcgggcat ctttctctat
      601 gtccttctcc gggtaaaggg ggaggagggg cttgaccttc ggggctggct cgaatcattc
      661 gtggtcggag cgatgctttt cataggagga gccggacttc tctgcgtcgg tcagcagtgg
      721 gtcgcctcag gactttcggc gacactgctg gcaaccacgc ccctgtggac agttctgttt
      781 gccggtatct gggagaagat gccaactacc atggagtggg ctggtctggg gataggtatt
      841 gccggtgttg cccttctaaa cctggacagg ggcatcagcg gcaaccctct gggcgccggg
      901 atcatacttg tcgcttccat catctggtcc ctgggttccg tgttgaacaa ccgcttccag
      961 ggcctgggaa aaccgatagc gtcagctacg gagatgattg cgggagggtt gctcctgctc
     1021 atcctgtccc ttcttgtcgg ggagaggatc tcctggcccc tgacggccag ggctacgatg
     1081 ggcatgttcc acctggtagt cttttcctcg atcatagggt tcaccctcta ccgcttcatg
     1141 ctcaggacgg taaggccggc ccttgcgacc agctacaccc tggtaaatcc catcacggcc
     1201 acggttctgg gagcactctt tgcaggcgag agcgtgtctc tcgtatcgat cgccgccatg
     1261 gcggtggtcc ttacgggagt ctttctggtc ttcagggcca gggctatctg agaaaggggg
     1321 aggaacatga gcctggacct tgtcattgaa agtgtctccg ccggagatct ttcggtcctt
     1381 accggagctg ggatgagcac cgcctcgggg ctccccgatt tccgggggaa ggatggactc
     1441 tggaagcaga aagacccccg cctcctggca tctctttcgg ccatggagaa cgatcgagcc
     1501 gaattcgtga acttttatcg ttacaggata aggatgttcg aaggagtaaa acccaacagg
     1561 gggcaccata tcctggctga gtgggaggcc agggggctcc tgaaaggggt gatcacccag
     1621 aacgtggacg gactccacca ggaggcgggc tcaagggagg tccacgaact gcacggcaac
     1681 ctcagaaggg tccggtgctc ccgttgcagg gccgcctacg aaagcagcct ctttcttgaa
     1741 cgggatgact gtccggcctg cggaggatcc ttgagacctg gtgtggtcct cttcggagag
     1801 atgctccccg aggggcccat ggacgcagcg gaagagctct ccgggaccgc ttcctccttc
     1861 ctggtcctgg gttcttccct ggaggtatcc cctgccaacg cctaccctat gctggccaag
     1921 agggcgggag cgaagctctt catcatcaac atgaccccga cccagctgga ttacatggcc
     1981 gatggggtga tccacggcga tatcgttgaa acccttgaga agatcgacaa gagcgtaacg
     2041 catgggtcat gaaagcgtaa gaggttagaa gcaagaagaa gagaaaaaaa gaagccgaga
     2101 ccgagattgc acaacagagg acacggttga tctgttcttg tctttcccgt tcacccttca
     2161 tgccttgaag ggcttgcggt caagcgttac gcccctactc cctccatctc tccaggttct
     2221 gtagatagag ccgtctcaga tgggccgaaa ctggacctgg cttgccgccg cctacggccg
     2281 tcttgccgat ccggacgacg gggaggatct cctttatgct accggtaatg aagaactcgg
     2341 cgcagagggg gagctcatcc cttaaagggc atctctcctc gacggggaag cctgcatccc
     2401 tggctatctc cagggtcatt ctcctcatgg tcccgggaag aac
//
LOCUS       DAOPKQ010000209         2430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_50179_length_2430_cov_3.584000, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000209 DAOPKQ010000000
VERSION     DAOPKQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50179_length_2430_cov_3.584000"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PK146_06765"
     CDS             <1..634
                     /locus_tag="PK146_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPE91975.1"
                     /translation="VMLVRGGGSRDDLNPFDEEEVVRAVRSCPVPVVTGLGHQVDRTL
                     SDLAADLFAPTPSAAAEALFPDRMDLLREIRGIRARMASGISREMHRHQMLLEGRVNL
                     LARSFERSALLPAGTALERLRTRAVTAAERILTLSWSSFREIASAMDALSPLKVLGRG
                     FVSCSSGDGNVVSRAAEVSAGDDLIVAFIDGRVQTTVRSVQESSGKGGGR"
     gene            638..1675
                     /gene="mtnA"
                     /locus_tag="PK146_06770"
     CDS             638..1675
                     /gene="mtnA"
                     /locus_tag="PK146_06770"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPE91976.1"
                     /translation="MEYHAVRWDDEEDMLVLLDQRELPSGVEHILCRDVECVARAIED
                     LAVRGAPAIGIAAAFGLVLGSGGKESELSHAARRLISTRPTAVNLFWAIGRMLERALA
                     VPEGVDLKKELLEEAKGILEEDIAANRTMGILGQALLPEKCSVLSHCNAGALATGGFG
                     TALGVIRAARERGKEVKVFLDETRPILQGARLSSWELAEDGFDVTVICDSMAGALMRE
                     RKIDAVIVGADRIASNGDVANKIGTYSLAVLAKEHWVPFYVAAPWSTFDTSLASGEDI
                     IIEERSGEEVRLLPGGKRIPDRIKVWNPAFDVTPAALVTAIISEKGIFRAPYDLKDMG
                     ENDPEGQDVII"
     gene            1672..>2430
                     /locus_tag="PK146_06775"
     CDS             1672..>2430
                     /locus_tag="PK146_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPE91977.1"
                     /translation="MKGSTIAEASAYREGERKILWAKQRMPVLAGLVGIHGRERPLSG
                     ITVGACLHLEAKTACLLLSLKELGAEVIAAGSNPLSTQDDVCAALVKGGVGVYSRRGM
                     TDDEYFRNLEQVLMSSPQIIVDDGADLVVMAHEKRPDLLPGIRGGSEETTTGVRRLKA
                     MAEQGVLAFPVISVNDARSKFLFDNRYGTGQSVFDGLMRATNVLLAGKVFVVAGYGWC
                     GRGVAMRAKGFGARVVVVETDPHKAFEALMDGCQV"
BASE COUNT          480 a          663 c          809 g          478 t
ORIGIN      
        1 cgtgatgctc gtaaggggag gggggagccg cgacgacctc aatcccttcg atgaagagga
       61 ggtcgttagg gccgtaaggt cgtgccccgt tccggtagtg acagggctgg ggcaccaggt
      121 tgacaggacg ctctcggacc tggccgctga tctcttcgct cccacaccat cggctgccgc
      181 agaggcactc ttccccgaca ggatggacct cctcagggag atccgcggga taagggcgag
      241 gatggctagc ggcatctcca gggagatgca ccgtcaccag atgcttctgg agggaagggt
      301 taacttgctg gcaaggtcct tcgaaaggtc cgccctcttg ccggccggga ctgcccttga
      361 gagactcagg acaagggcgg tgacggcagc cgagcggatc ctcacccttt cctggtcctc
      421 cttcagggag atcgcttcgg ccatggatgc cctctctcct ctcaaggttc tgggcagggg
      481 ttttgtctcc tgttcttcgg gcgacgggaa cgtcgtctcc cgtgctgccg aggtctccgc
      541 tggtgatgac ctgatagtgg cctttatcga cggaagggtc cagaccaccg tgagatccgt
      601 ccaggagagc tccggtaaag ggggaggtag ataaaccatg gagtaccacg ctgtccgctg
      661 ggacgatgaa gaggatatgc tggtgttgct cgaccagagg gaactcccct ccggggtcga
      721 acatatcctc tgccgcgacg tcgagtgcgt tgcccgggcc attgaagatc ttgccgtcag
      781 gggggctcct gcgataggga tagctgcggc ctttggtctg gtcctgggat cggggggcaa
      841 agagagcgag ttgagccacg ccgcccgaag gcttatatcg acaagaccta cggcggtcaa
      901 cctcttctgg gcgatcggga ggatgcttga gagggctctt gccgttcctg agggagtgga
      961 cctgaagaag gaacttcttg aagaggctaa agggatactg gaggaggata ttgccgccaa
     1021 ccggaccatg ggtatcctgg ggcaggccct tctccccgaa aagtgcagcg tgctgagcca
     1081 ctgcaacgct ggtgccctgg ccacgggggg attcggcacg gctcttggcg tcatcagggc
     1141 ggcaagagag cgcggcaagg aggtcaaggt ctttctcgac gagacgagac cgatccttca
     1201 gggtgcccgt ctctcctcct gggaactggc cgaggatggt ttcgatgtca ccgtcatctg
     1261 cgactccatg gcaggtgccc tgatgaggga gagaaagatc gacgccgtta ttgtcggggc
     1321 cgacaggata gcctccaacg gggatgtggc caacaagata ggcacctact cgcttgccgt
     1381 ccttgctaag gagcactggg tacccttcta cgtggcggcg ccctggagca cttttgacac
     1441 ttccctcgcc tccggggagg atatcataat agaggagcgt tcgggtgaag aggtacggct
     1501 tctcccagga gggaagagga tccccgacag gatcaaggta tggaacccgg ccttcgacgt
     1561 tactcctgcg gcgctcgtta cggccatcat cagcgagaaa gggattttca gggcccccta
     1621 cgatcttaaa gacatgggtg aaaatgaccc ggaaggacag gatgtgatca tttgaaggga
     1681 tccacgatag cagaggcctc cgcatacagg gagggagaga ggaagatact ctgggcgaag
     1741 cagagaatgc ccgtactggc agggctcgtc gggatccatg gcagggagag gcccctttct
     1801 ggcattaccg tcggtgcatg tctgcatctt gaggccaaga ccgcctgcct cctgctctcc
     1861 ctcaaagagc tgggcgcaga ggtgattgcc gcgggcagta accctctctc cacccaggat
     1921 gacgtttgcg ctgccctcgt taagggaggg gtaggcgttt acagccgcag gggaatgacc
     1981 gacgacgagt atttcaggaa tctggagcag gtcctcatgt cctccccgca gattatcgtt
     2041 gatgacggcg cagacctcgt tgtcatggcg catgagaaga gacctgacct tctccctggc
     2101 atcaggggag gctctgagga gacgacaacg ggggtcaggc gactgaaggc gatggccgag
     2161 cagggggtcc tcgcctttcc ggtgatctcg gtcaacgacg ccaggagcaa attcctcttc
     2221 gacaaccgtt acggaacggg tcagtccgtc tttgacgggc tgatgagggc gaccaacgtc
     2281 cttcttgcag gaaaggtttt cgtcgttgca ggctacggct ggtgcggacg cggcgtggcc
     2341 atgagggcca agggtttcgg cgccagggtt gtggtcgtcg agaccgatcc ccacaaggct
     2401 ttcgaagcgc tgatggacgg ctgccaggtc
//
LOCUS       DAOPKQ010000210         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_51076_length_2401_cov_2.322677, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000210 DAOPKQ010000000
VERSION     DAOPKQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51076_length_2401_cov_2.322677"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PK146_06780"
     CDS             <1..907
                     /locus_tag="PK146_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPE91978.1"
                     /translation="ELNYRGLGHRVEIGFETGDREQYWLTYTEPYMDEYHYSWRVGVY
                     RREWDDLRDYSLGVYNATYDQDKQGIFLGAGKKFRHDPTLSWFVLLDWHEVEINVTDG
                     SLPPGKELDGRNFSVTGTLARNRLDPYLSYPKGDFLSLNVEQGLSSLGGDWDYTKYWF
                     EARYYWPLYHLFEEFLDREIGTEENPVIFASRIRVGYSSGDLPWAEQFFLGGASTLRG
                     YRDDEFYGDEMALGNFEIRIPVQEAISVVGFYDIGMAGDSAFSDLKSAYGFGFRVRTP
                     IGNIRLDFGTGDYETRTHFSFGEMF"
     gene            924..2174
                     /locus_tag="PK146_06785"
     CDS             924..2174
                     /locus_tag="PK146_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91979.1"
                     /translation="MRYDQFLHRAAAGFFAVALLFTAPAFASVEVAGVPEWIRPTIQG
                     PVDAVWEEVLRSRDPGSGLQTLRIVVERIFPGVVMEGIELSGEDLRLDLSFEDPPPGG
                     WEVSVKLPDLVPELEDLFNRDMGDVAGQLREMIAPLPVESLSWAGQAFQQETVRMLTS
                     LVPGWHPSILFVKGRGGEIEVLVSFNPLSPVIIAYSPRISSRTLPRILQTEITDNALE
                     AMAPFIGLPGDWVDRHRLSIEELVAGILETKWAAREVRGEVKVGITPERIAPVDIRVE
                     SDRYTLQAWAAVHLGSDERHPEINVHIGRMTSPIRGWDLEIYGEFVATTNDLGLESRW
                     GARWSAWPDIWVGAEIAYPGEDVWFRVWLDEILPRVYLWGRLNGEGDSVAGLGWRFGG
                     YLAWELYYDSRDEDRISVRLVGNL"
     gene            2211..>2401
                     /locus_tag="PK146_06790"
     CDS             2211..>2401
                     /locus_tag="PK146_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91980.1"
                     /translation="MDLNKEGTISQLAEILGGHPFGDTDLVVRGIQGTDGEDPSKIIV
                     YRDTRPARMPSAGIPAVLPP"
BASE COUNT          513 a          600 c          741 g          547 t
ORIGIN      
        1 ggaactgaac tacaggggac ttggccacag ggtagagatc ggctttgaga caggcgacag
       61 agagcagtac tggctcacat atacagaacc ctacatggac gagtaccatt acagctggcg
      121 agtcggggtt tacaggcggg agtgggatga cctaagggat tattcccttg gtgtttacaa
      181 cgcaacctac gatcaggaca aacaggggat cttcctgggt gcggggaaga agttccgcca
      241 cgatcctact ttgagctggt ttgttcttct tgactggcac gaagttgaga tcaatgtgac
      301 cgacggatcg cttccccctg gcaaggaact tgatggccgg aatttttctg ttactggtac
      361 ccttgcccgc aatcgcctcg acccttatct gagttatccc aagggagact ttttgagcct
      421 taatgttgaa caggggctca gttccttggg tggcgactgg gattacacta aatactggtt
      481 tgaagcccgt tactactggc ctctatatca tcttttcgag gaattcctgg acagggagat
      541 cggtacagaa gagaacccgg tcatattcgc ctccagaata agggtgggtt actcttcggg
      601 tgatctcccg tgggcggagc agttcttcct cgggggggcg agcaccctga gaggatacag
      661 ggatgacgaa ttctacggtg atgaaatggc tcttggaaac ttcgagatcc gtattcctgt
      721 tcaggaagcc atttctgttg tcggcttcta tgacattggt atggcgggag attctgcctt
      781 ttctgatctg aagagtgcat atggtttcgg ttttcgtgtc aggacaccga tcgggaacat
      841 ccgactcgat tttggcactg gggattacga gacccggacg cacttcagct ttggagaaat
      901 gttctagtcg ggagacctct attttgagat atgaccagtt tctccacagg gccgcggccg
      961 gctttttcgc cgtggccctg cttttcacgg caccagcttt tgcttcagtg gaagttgcag
     1021 gggtcccgga atggatccgc ccaaccatac agggacctgt ggatgctgta tgggaagagg
     1081 tccttcgttc aagggatccc ggctcgggtc tgcagaccct ccgtatagtt gttgagagaa
     1141 ttttcccggg agtagtcatg gaaggcattg aactgtcggg tgaggatctt cgccttgacc
     1201 tctcctttga ggatccgccc cccgggggat gggaagtatc cgtcaagctt cctgaccttg
     1261 tccccgaact tgaggacctt tttaacaggg atatgggaga tgtcgctggt caactcaggg
     1321 agatgatagc gccgttgccg gtggagtccc tctcctgggc agggcaggca ttccagcagg
     1381 aaacggtcag gatgctcaca tcgcttgtcc ccggatggca tccatcgata ctcttcgtaa
     1441 agggcagggg aggagagatc gaagtcctgg tcagctttaa ccccctttcg ccggtaataa
     1501 tcgcctattc gcccaggatc agctccagga cccttcccag gatcctgcag acagagataa
     1561 ccgacaacgc cctggaggcc atggcccctt tcataggact tccaggcgat tgggttgatc
     1621 gccacaggct gtcgatcgag gagttggtcg cagggatcct ggagacgaaa tgggcggccc
     1681 gggaggtccg gggagaggtc aaagtcggga taactcccga acggatcgcc ccggtggata
     1741 taagggttga gagcgacagg tacaccctcc aggcctgggc ggcggtccac ctggggtctg
     1801 acgaaaggca cccggagata aatgtgcaca taggcaggat gacctcaccg atcaggggct
     1861 gggaccttga gatctacggc gaatttgttg ccacaacaaa cgatcttggc ctggagagcc
     1921 gatggggggc gaggtggtca gcctggcctg acatctgggt aggagccgag atagcctatc
     1981 cgggggagga tgtatggttc agggtgtggc ttgatgagat cctcccccgg gtctacctct
     2041 ggggtcgtct gaacggagag ggggactccg tcgcagggct cggctggcgt ttcggcggct
     2101 acctggcctg ggagctctac tatgacagcc gtgacgagga caggatcagc gtaaggctcg
     2161 tcgggaacct ttgatcattt atatgagatt gaaacaaagg gagaagaggc atggacctca
     2221 acaaggaagg gacaatttcc caactggcag agatcctggg tggtcaccct ttcggtgaca
     2281 ctgatcttgt ggtaaggggc atccagggca cagacgggga ggacccctcg aagatcatcg
     2341 tctacaggga cactcgcccg gccaggatgc cttctgcggg tatccccgcg gtcctcccgc
     2401 c
//
LOCUS       DAOPKQ010000211         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_51123_length_2399_cov_3.854522, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000211 DAOPKQ010000000
VERSION     DAOPKQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51123_length_2399_cov_3.854522"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PK146_06795"
     CDS             complement(<1..631)
                     /locus_tag="PK146_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPE91981.1"
                     /translation="MVDLRVGHSEVGVHGKMRVVNAVNRFQDIAGEHADSLGLGVRDL
                     LPKGYTWVLHQLRLSFSRAAVAGEDLTIRTWYRPERNLYSLRNFSMEDANGKRIIFGE
                     SSWVVLDLKKGRPVRLSTAMPASYEENRSEDFPTGFSEIPELDEPDTEILFNVRLHDL
                     DINRHVNNARYLEWALEAIPMEVMGSSRPAVVEVLFRTPARYGEVIVSQV"
     gene            complement(679..1656)
                     /locus_tag="PK146_06800"
     CDS             complement(679..1656)
                     /locus_tag="PK146_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPE91982.1"
                     /translation="MGKRDNKLNVAIIQETPVFLNLKASVEKACSLTREAAREGARVV
                     AFPETWLPGYPVWIDSAPHAAIWGDDTARGLYRRLVENSLTIPGEEFGILVSTATEAG
                     VHMVMGAHELIGSTLYNTMLFISPDGTYRTHRKLTPTYTERLVWGMGDGSTLVSVDAD
                     FGVLGGLICWEHWLPLARAAMHARREVVHVAQWPWVRDLHQVASRHYAFEGQCFVLAA
                     GTVLTLDNVLEGYGSLPGVTTGGKEMLSSMGRAGELLQRGGSTVIAPDSTCIVEPLFD
                     ACGTIHAEVDLDLVTEGHLLMDSDGHYSRPDVFSLTVNTAPMQGVRFVP"
     gene            complement(1790..2293)
                     /locus_tag="PK146_06805"
     CDS             complement(1790..2293)
                     /locus_tag="PK146_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPE91983.1"
                     /translation="MKKEEILLIAKETEDLLVPFCSCGSLGLHGLSHLRRVAILSGRL
                     ASATGEDVEAAVVMGFLHDGARTDDKGGNGHAHDSAVLARQLLKMFYPHLEADRICDA
                     IARHADGEVTGDTLTACLWDADRLELKRIGRTIDLDLLSTRVAKRLARARAARMVPVA
                     LEGRQSR"
BASE COUNT          497 a          763 c          637 g          502 t
ORIGIN      
        1 ttacctggga gacgataacc tcgccgtaac gggctggcgt tcggaaaaga acttctacaa
       61 ccgcaggcct ggatgaaccc atcacctcca tgggtattgc ctccagggcc cattcaaggt
      121 aacgggcgtt gttaacgtgg cggttgatgt caagatcgtg aaggcgcaca ttgaaaagga
      181 tctcggtatc gggttcgtcc agttccggta tctcgctgaa accggtcggg aaatcctcgg
      241 acctgttctc ttcgtaggac gcgggcatgg cagtactcag cctcaccggg cgccctttct
      301 tcagatccag gacaacccag gaggattccc cgaaaattat cctcttgccg ttcgcgtctt
      361 ccatagagaa attcctcagc gagtagaggt tcctttcagg gcggtaccat gtacggatcg
      421 tgaggtcttc tcctgccact gccgccctgc tgaagctcag cctcaactgg tgaagcaccc
      481 atgtataccc cttcgggagc aagtccctta cccccagccc caggctgtcg gcgtgctcac
      541 cggcaatgtc ctggaaccgg ttgacggcgt taaccaccct catttttccg tgaaccccca
      601 cctccgagtg cccaaccctg agatcaacca tgtactccac cggctatcgc caccttttac
      661 agaaaaaggg tcactggatc aggggacgaa ccttactccc tgcatggggg ccgtattcac
      721 tgtcagggag aagacatcgg gcctggagta gtggccatcg gaatccataa gcaggtgccc
      781 ctcggtcaca aggtccagat ccacctcggc gtggatcgtt ccgcaggcgt caaaaagggg
      841 ctcaacgata caggtcgagt cgggggcgat cacggtgctc ccgcccctct gaagcagttc
      901 gcctgccctg cccatggagg agagcatctc ctttcccccg gtcgttaccc ccgggaggga
      961 tccgtacccc tcaagcacat tgtccagggt caggaccgta ccggcggcaa gaacgaagca
     1021 ctgaccctcg aaagcataat gcctgctggc gacctggtgg agatccctca cccatggcca
     1081 ctgggcaacg tgaaccactt ccctcctggc gtgcatggca gcccgggcaa ggggaagcca
     1141 gtgctcccag cagataagac cgcccagaac accgaaatcg gcatctaccg aaacaagggt
     1201 gctcccgtcg cccatacccc agaccagacg ctccgtgtag gtaggggtga gcttcctgtg
     1261 ggtcctgtaa gtcccgtcgg gggagatgaa gagcatggtg ttgtagaggg tgctgccgat
     1321 aagctcgtga gcgcccatga ccatgtggac gccagcctcg gtggcggtgc tgacaaggat
     1381 cccgaactct tcaccaggga tggtaaggga attctccaca agcctgcggt aaagtcccct
     1441 ggcagtgtcg tcaccccaga tcgctgcatg gggagccgaa tcgatccata cggggtagcc
     1501 tgggagccat gtctcgggaa aggccaccac cctggcgcct tctctggctg cttcccgggt
     1561 cagactgcag gccttctcca cgctggcctt caggttcagg aataccgggg tttcctgaat
     1621 tatcgcgacg ttcagtttgt tgtccctttt tcccataact gcctcctcaa gtcacgtgga
     1681 tcggcgtctg cgattatatg aggagatcat ccccacatat tccgacgcaa gatccttgcc
     1741 cttagcccac ggatacttcg acgctattgt cagcatcgac tcctgccact cagcgggact
     1801 gtcttccctc cagggcgacc ggcaccatcc tggccgccct ggcccttgca aggcgtttgg
     1861 caactctcgt ggatagcagg tccagatcta tggtcctgcc tatgcgtttc agctccagcc
     1921 tgtcggcatc ccagaggcag gccgtcagcg tatccccggt gacctctcca tcggcgtgac
     1981 gggcaatggc gtcacagatc ctgtcggctt caaggtgggg gtagaacatc ttcagcagct
     2041 gcctggcaag gaccgccgaa tcatgggcgt ggccgttgcc ccctttgtcg tccgtcctgg
     2101 cgccatcgtg caaaaaaccc atcactaccg ccgcctccac gtcctcccct gtagctgagg
     2161 ccagcctccc cgaaaggata gcaacccttc tcaagtgaga tagcccgtgc agccccagac
     2221 ttccacatga acagaaaggg accagtagat cctctgtctc ttttgcgata aggagaatct
     2281 cttctttttt catcttcaga cccttttcca taattccccc catgcatgaa agataccgga
     2341 taccatccgc ctgaccatcg gccctctggc cgacagagaa aaccacttat caacgtaca
//
LOCUS       DAOPKQ010000212         2397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_51193_length_2397_cov_3.512383, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000212 DAOPKQ010000000
VERSION     DAOPKQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51193_length_2397_cov_3.512383"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PK146_06810"
     CDS             complement(<1..622)
                     /locus_tag="PK146_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPE91984.1"
                     /translation="MPTSVIKPVMSAEEAVKRITDGSSVMIGGFNYGGIPYTLVEALV
                     DAGTKDLWLISNDTAYDDVGHGRLVVNGQVKKVTASHVGLNKSTQRLFNEKKMELELV
                     PQGTFVERIRAGGFGLGGVLTPTGVGTVHEEGKQVIEVEEKRYILELPLRADVALIRA
                     YRADRYGNLVYFGTNRNFNPAMATAADLVIAEVDAILEIGQINPNDV"
     gene            complement(624..>2397)
                     /locus_tag="PK146_06815"
     CDS             complement(624..>2397)
                     /locus_tag="PK146_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPE91985.1"
                     /translation="RAIHWMMMGAIIFLLYPFSRKRPRDRIDIWDWALAALLVAGCLN
                     ILLNWGAITAREGRSIPSDIYMGIAMTFLVIEATRRTVGWPLPIVALVAISYAVFGRS
                     LPGMLKHPGFPVSELAPFLYLRTDGIFGVPLGVSATFIFLFVLFGSVLNVSGAGSFFI
                     DLAMSLTGRSRGGPAKAAVVASALMGSVSGSSVANTVTTGSFTIPLMKKVGYPAHFAG
                     AVEAAASTGGQIMPPVMGAAAFVMAQFLGISYWSIVVAAVIPACLYFFSVFAMVHFRA
                     GKEGIEGIPKKDLPKVWEVLKKGWMLLLPVVTLIVFLALGYSAIKAVFWSIMLLIGAS
                     WLGPPEYRMTPRRIVNSFIDGGVTAVEVAAACACSGIIIGVVGITGLGLAFTSFIIGL
                     SRGILFLALVLTMIASIILGMGLPTTAKYIILSTLAAPAIHRMGVPMLAAHLFILYFG
                     VIADVTPPVALAAYAGAGIAGANAMRTGFNALGLAIAGFIVPFMFAYNQALLFQGSLV
                     NVLLSSVTAILGVIALAAGVQGFYLGRLNMPERVLFVATAIALIKPGILSDVIGIVVL
                     GGIYLLQRRSLKVKKNKETSGEEI"
BASE COUNT          581 a          695 c          641 g          480 t
ORIGIN      
        1 caacatcgtt ggggttgatc tgaccaatct ccaggattgc gtccacctcg gctattacaa
       61 ggtcggcagc ggttgccatg gcggggttga agttgcgatt ggttccaaaa tagaccaggt
      121 taccgtacct gtcggccctg taggccctta taagggcaac atcagccctg aggggaagtt
      181 caaggatgta tcgtttctcc tccacctcga tgacctgttt cccctcctcg tggaccgtcc
      241 ctactccggt aggcgtcagg acccccccga ggccgaaccc tcccgccctt attctctcaa
      301 caaaggttcc ctgcggcaca agttcaagtt ccattttttt ttcgttaaaa agacgctggg
      361 tgcttttgtt gagcccaaca tgagaggcgg tcactttttt gacctgaccg ttcaccacca
      421 gccggccatg acctacgtcg tcataggccg tatcgttcga gatcagccaa aggtcctttg
      481 tcccggcatc gaccagcgct tcgacgaggg tatatggaat acctccgtaa ttgaacccgc
      541 ctatcatgac cgaactgccg tcagtgatcc ttttgacggc ttcttcggca gacatgaccg
      601 gcttgatgac gctcgtaggc atttcagatc tcctccccac tggtttcctt gttcttcttc
      661 acctttaaag atcttcgctg aagaaggtag atccctccca gaaccacgat cccgatgaca
      721 tccgacagga tcccgggctt gatcagcgca atggcggtcg cgacaaagag gaccctctcc
      781 ggcatgttga gtcggccgag atagaatccc tggacccctg cggccagagc gatcacaccg
      841 aggatcgccg taacgcttga aagaagtacg ttaaccaggc ttccctggaa cagaagagcc
      901 tggttgtaag cgaacatgaa agggacgatg aacccggcta tcgcaagacc cagggcattg
      961 aagcccgtgc gcatggcgtt tgcccctgcg atccctgctc ccgcgtatgc ggcaagagct
     1021 accgggggag ttacatcggc aattacaccg aaatagagga tgaaaagatg ggctgcaagc
     1081 atcggcaccc ccattctgtg tatagccggt gccgccaggg tggagaggat aatgtacttt
     1141 gcggttgtgg gcaggcccat acccaggatt atggaagcta tcatggtcag taccagggcg
     1201 aggaagagga taccgcggga gagaccgatg ataaaagaag taaaggcaag accaagaccg
     1261 gtgatcccca ccactcctat gattattccg gaacatgcgc aggcagccgc cacttccacc
     1321 gcagtgacac ccccgtctat gaaggaattg acgatcctgc ggggggtcat cctgtactcg
     1381 ggaggcccaa gccagctggc tccgataaga agcatgatcg accagaagac ggccttgatt
     1441 gccgagtacc ccagagccag aaagacaatg agggtgacca cagggagaag cagcatccag
     1501 ccctttttca ggacctccca gactttgggg aggtcctttt tcggaatgcc ctcaataccc
     1561 tctttgcccg cacggaaatg gaccatggca aaaaccgaga aaaagtagag gcaggctggt
     1621 atcacggccg ccaccacgat gctccagtag gagatcccca ggaactgggc cataacaaag
     1681 gccgcagccc ccattactgg cggcatgatc tgacctcctg tagatgccgc cgcctcaacg
     1741 gcaccggcaa aatgtgccgg atacccgacc ttcttcataa ggggtatcgt gaaagatccg
     1801 gtggtgaccg tatttgcaac ggagctcccg gaaacactcc ccataagggc actagcgaca
     1861 acagctgcct ttgccggccc acccctgctc cttcctgtga gggacattgc aaggtcgata
     1921 aagaaacttc cggccccgga aacgttcagc accgatccga agaggacgaa aaggaatatg
     1981 aaggtggccg agacacctag agggacaccg aatatgccgt ccgttctgag atacaggaag
     2041 ggagccagtt cgcttacggg gaatcccggg tgtttcaaca tcccgggaag agaacggccg
     2101 aaaaccgcat aagatatggc taccagggca acgatcggaa gaggccaccc cacggtcctc
     2161 ctggtggcct cgatcacaag gaaggtcatg gcgataccca tgtaaatatc ggacggtatc
     2221 gatcgtccct cccttgctgt aattgccccc cagttcaaga ggatgttaag gcaccccgcg
     2281 accagtagtg ccgccagcgc ccagtcccat atatcgatcc tgtccctggg tcgtttcctg
     2341 gagaaagggt aaagcagaaa gatgatggca cccatcatca tccagtggat cgctctc
//
LOCUS       DAOPKQ010000213         2394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_51272_length_2394_cov_5.136811, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000213 DAOPKQ010000000
VERSION     DAOPKQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51272_length_2394_cov_5.136811"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK146_06820"
     CDS             complement(<1..534)
                     /locus_tag="PK146_06820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPE91986.1"
                     /translation="MSWKRTADVVVIGAGVQGCSIAYNLAKMGVKDIVVLEKGTICSG
                     STGRCGAGIRAQWGTEMNCRFGLAAVGKFEQLHEELGMDCGLNQSGYLMVAYKDSEFE
                     QLQKSMKLQNSLGINTRVVTYDEAKEICPGLAAEDAVGFTFHDRDGHADPFLTTFAYM
                     EAAKRLGVKFLRFTEATD"
     gene            complement(534..815)
                     /locus_tag="PK146_06825"
     CDS             complement(534..815)
                     /locus_tag="PK146_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPE91987.1"
                     /translation="MAGKNEDIIICRCEEVTLGEIREWIEKGYETFDELKRVLRVGMG
                     PCQGRGCREMILREISAKTKVSVADIHPGTFRPPAKPVKLAVLAKGGDK"
     gene            complement(818..1327)
                     /locus_tag="PK146_06830"
     CDS             complement(818..1327)
                     /locus_tag="PK146_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPE91988.1"
                     /translation="MNEKEKLFNSGVLVETRKGAVEPPVDLWQNKKNGLVIIECPQRI
                     PCNPCHTSCPTGAVKPFRDINDQPEIDYEKCTGCARCVAVCPGLACFVVDLTWGDEDT
                     ALMKLPYEMLPVPEEGKSVDCLNRVGEPISRGKVVKVLEPMHDRTMVVHVEVPRSKVM
                     EIRTIRVVR"
     gene            complement(1332..>2394)
                     /locus_tag="PK146_06835"
     CDS             complement(1332..>2394)
                     /locus_tag="PK146_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPE91989.1"
                     /translation="AGLSASLEAASLGCQVTLVDSDLALGGQLIKQTHKFFGSKDERA
                     GTRGFKISEILLEELDGYKDRVKVHMNCTATGFYREDGVVSCVIGEDEYFRIKPGKIV
                     MATGAQERLLPFPNNHLPGVYGAGAVQTLMNVYGVAPGKRVLMVGAGNIGLIVSYQLM
                     QAGVEIAGIVEAMDRVGGYWVHAAKVRRMGVPIYLRHTIKEALGDRVIAGAMIQEIDD
                     KFQPVGKEIQVDADIICMAVGLTPTCELLWQAGCEMVFVPQLCGHVAQRDLFMRTSHP
                     DIWVAGDAAGIEEASAAMVEGRIAGVGVARSLGKGDSSEHSKRLEEYRRRLADLRGGE
                     VGQKICQGLECCTVNEWGV"
BASE COUNT          462 a          769 c          594 g          569 t
ORIGIN      
        1 atcggttgct tctgtgaagc gtaaaaattt cacgcccagt cttttcgccg cttccatgta
       61 ggcaaaggtc gtaaggaacg gatcagcgtg tccatccctg tcgtggaaag taaaaccgac
      121 agcatcctcg gccgcaagac ccggacagat ctcctttgcc tcatcatagg taactaccct
      181 ggtattgatc ccgaggctgt tctgaagctt catgctcttc tggagctgct cgaattcgct
      241 gtccttgtag gccaccatca ggtaaccgct ctggttcagg ccacaatcca tccccagctc
      301 ttcgtgcagc tgctcgaact tccctaccgc tgcaagcccg aacctgcagt tcatctccgt
      361 tccccactgg gccctgatcc cggcgccgca tctcccggta gaacccgagc aaatggtccc
      421 tttttcgaga acaacgatat ccttgactcc cattttcgcc aggttatagg caatggagca
      481 accctggacc ccggctccga tcacaacgac atcggctgtc ctcttccagg acattacttg
      541 tctcccccct tggcgagaac ggccagtttg accggcttcg caggaggcct gaaggtcccg
      601 gggtgaatat cagcgacaga aacttttgtt tttgccgata tctccctcag gatcatctca
      661 cggcatcctc tcccctgaca cggtcccatg cctacccgta ggactctctt tagttcgtcg
      721 aaggtctcgt aacccttctc tatccactcc ctgatctcac ccagggtgac ctcttcacaa
      781 cggcagatga tgatatcttc gttctttccg gccatgacta cctcaccacc ctgatcgtgc
      841 ggatttccat cacctttgat ctggggacct cgacatggac gaccatcgtc ctgtcgtgca
      901 tgggttcgag gacctttacg accttgcccc tggatatggg ttcgcccacc ctgttcaggc
      961 agtccacgct cttgccctct tcggggacgg ggagcatttc gtagggaagc ttcattagcg
     1021 ccgtatcctc atcgccccag gtcaggtcga ccacgaagca ggcaaggccg gggcagaccg
     1081 ccacgcacct tgcgcaacct gtgcattttt cgtagtcgat ctcgggctgg tcattgatat
     1141 ccctgaaggg ttttactgct ccggtaggac agctggtatg acaggggttg cagggtatcc
     1201 tctggggaca ttcgatgatg accagaccat tcttcttgtt ctgccagagg tccacagggg
     1261 gttcaactgc ccctttgcgc gtttccacga gcaccccgct gttgaagagc ttctcttttt
     1321 cgttcaatgt atcacacccc ccactcgttg acagtgcagc attcaaggcc ctgacagatc
     1381 ttctgtccga cctcgccacc cctgaggtcc gcaagtcgtc ttctgtattc ctcgaggcgt
     1441 ttcgaatgct ccgaactgtc gcccttcccg agagacctgg ctacaccaac acctgcgatc
     1501 cggccctcga ccatggcggc cgatgcctcc tcgatgcctg cggcgtcacc ggcgacccag
     1561 atatcggggt gactggtcct catgaagaga tccctctgtg caacgtggcc gcaaagctgg
     1621 ggaacaaata ccatctcaca accggcctgc cagaggagtt cacaggtcgg ggtgagaccg
     1681 acagccatac agatgatgtc ggcatccacc tggatctcct tgcccacggg ctggaatttg
     1741 tcgtcgatct cctggatcat cgcccctgcg atgaccctgt cccccagggc ttcttttatg
     1801 gtatgcctga ggtagatggg gacccccatc cttctcacct tggcggcgtg aacccagtaa
     1861 cctccaaccc ggtccatggc ctcgactatg cctgcgatct cgacacccgc ctgcatcaac
     1921 tgataactga cgataagacc gatatttccg gctccgacca ttaggactct cttgccgggt
     1981 gccactccgt agacgttcat aagggtctgg accgcccccg caccatagac gccggggaga
     2041 tggttgttgg ggaacgggag aaggcgctcc tgggctcctg tggccataac gatcttgccg
     2101 ggtttgatcc tgaaatactc gtcttctccg ataacacagg aaactacccc gtcctcccgg
     2161 taaaatccgg ttgcggtgca gttcatgtgg accttgaccc ggtccttgta cccgtcaagc
     2221 tcctcgagaa ggatctcgga gatcttgaac ccccttgtcc cggctctctc atcctttgag
     2281 ccgaagaact tgtgtgtctg cttgatcaac tgtcctccaa gggcgaggtc gctatccacc
     2341 agagttacct gacaccccag cgaggctgct tcaagggagg cgctgagacc tgcg
//
LOCUS       DAOPKQ010000214         2388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_51470_length_2388_cov_3.894985, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000214 DAOPKQ010000000
VERSION     DAOPKQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51470_length_2388_cov_3.894985"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PK146_06840"
     CDS             <1..348
                     /locus_tag="PK146_06840"
                     /inference="COORDINATES: protein motif:HMM:NF021883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPE91990.1"
                     /translation="GMTVLDEVMKRNLDLSKGLVFSQRVLLGLVEKGFSREDAYAIVQ
                     GAAMQCWDQDRSFLEVLEDDPRVAGAFKGEELHSLFDTEYYLRFIDGIFERFPVKDPS
                     FTKEQATRKERYE"
     gene            345..1340
                     /gene="glpX"
                     /locus_tag="PK146_06845"
     CDS             345..1340
                     /gene="glpX"
                     /locus_tag="PK146_06845"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HPE91991.1"
                     /translation="MNKPERNMALELVRATEAAAMAAGRWMGRGDRNASDNAAVNGMR
                     YMLNTIEMNGTIIIGEGEKDEAPMLFNGEKLGTGNNPEVDIAVDPIDGTRLLSLGRPN
                     AVSVVALSERNTMFDPKHIFYMNKIAVGPSAAGVIDIEAPVYENLSRVAKALDKAVED
                     VTVVVLDRPRHEQLISEIREAGSRIRLISDGDIAGALMTCKNGSGIDLLMGVGGSPEA
                     VISACALKCVGGTMECKLWPRNTEEAARCRELGMDLNAVLSLDDLVSSDNVFFAATGV
                     TDGEMLKGVRYEGDRIVTNSIVMRSKSGTIRYIDAIHSLRKLDLISSIDYGPRFE"
     gene            complement(1402..1734)
                     /locus_tag="PK146_06850"
     CDS             complement(1402..1734)
                     /locus_tag="PK146_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE91992.1"
                     /translation="MILDWEPAVRWILLEGLRLGAARTSSAFHFGLAEAISDIALEIS
                     RRTGVRETVLSGGVWQNRRLLSLAIPLLERRGLRPLIHHALSPNDECISVGQAAVASV
                     HWGGRTTT"
     gene            complement(1752..>2388)
                     /locus_tag="PK146_06855"
     CDS             complement(1752..>2388)
                     /locus_tag="PK146_06855"
                     /inference="COORDINATES: protein motif:HMM:NF036810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPE91993.1"
                     /translation="LDTAVYYEKALRHFIRLFGLRPNLLAADLHPSYACTDIAQKVMG
                     KPEDTLLVQHHHAHMAACLVENGHEGTVLGIILDGTGLGSDRNIWGGEFLLGDISSFT
                     RVGHFREAPLPGGDRAVLEPWRFALALMEKALGPGDAERLAGEIWPTHSHLFGSILST
                     LDKAPVTSSCGRLFDAVAAVLGIRDVISYEGQAAMELESSARGDPVQPLSG"
BASE COUNT          544 a          644 c          724 g          476 t
ORIGIN      
        1 ggaatgaccg tcctggatga agtgatgaaa aggaatcttg acctctcgaa agggcttgta
       61 ttcagccagc gggttctcct ggggctggtg gagaagggct tctcccggga ggatgcctat
      121 gctattgttc agggtgcggc catgcagtgc tgggaccagg acagatcttt tctggaagtg
      181 ctggaggatg acccacgcgt cgcaggggcc ttcaaggggg aggagttgca ttcgctcttc
      241 gacaccgaat actatctccg gttcatcgac ggaatcttcg agaggttccc tgtaaaggac
      301 ccctccttca cgaaggaaca agcgacgaga aaggagaggt atgaatgaac aagcccgaaa
      361 gaaatatggc tcttgaactg gtcagggcaa cagaagcagc agcgatggct gccgggcgct
      421 ggatgggaag gggtgaccgc aacgcctcgg acaatgctgc cgtcaatggt atgcgataca
      481 tgctaaacac aatagagatg aacggcacga tcattatcgg agaaggagaa aaggacgagg
      541 ctcccatgct ctttaacggt gagaagcttg ggactgggaa taaccccgag gtggatatcg
      601 ctgtcgatcc catcgacggc accaggctgc tctctctcgg gaggcccaat gccgtgagcg
      661 ttgtcgccct ttctgaacgg aacaccatgt tcgacccaaa gcacatcttc tatatgaaca
      721 agatcgcggt aggaccttcc gcagccggtg tgatagacat cgaggcccct gtctacgaaa
      781 acctctccag ggtggccaag gctctagaca aggctgtgga ggacgtgaca gtggtcgttc
      841 ttgacagacc caggcatgaa cagctgatct ccgagatcag ggaggcgggg tcaaggatcc
      901 ggctgatatc ggatggggat atcgccggag cgctgatgac ctgcaagaac ggaagcggta
      961 ttgatctcct gatgggcgtc ggggggtcgc ccgaggctgt tatcagcgcc tgcgccctga
     1021 agtgcgtcgg gggcaccatg gagtgcaaac tatggccaag gaacactgag gaggctgcca
     1081 ggtgcaggga actgggtatg gacctcaacg ccgttctatc ccttgacgac ctggtctcct
     1141 ccgacaacgt cttcttcgca gctaccggtg ttacagacgg ggagatgctc aagggagtaa
     1201 ggtacgaggg agacaggatc gtgaccaact cgatcgtaat gcgttccaag agcgggacca
     1261 taaggtacat tgatgcgatc cacagcctca ggaaactaga tctgatcagc agtatagatt
     1321 acgggccgag attcgaatag gggtggtcca tcggtcggca agacgcacag ggtccttaag
     1381 cgggccctgt acctggaatg actatgtggt ggtccggccg ccccagtgta cggaagccac
     1441 tgccgcctga cccacggaga tgcattcgtc gttgggagac agggcatgat ggatgagggg
     1501 ccttagtccc ctcctttcaa gaagggggat cgccaggctg agcagccttc tgttctgcca
     1561 aaccccaccg gaaaggaccg tttccctgac tcccgttctc ctggagatct caagggcgat
     1621 atcagagata gcctctgcaa ggccgaagtg aaaagccgat gatgtcctgg cggcacccag
     1681 acggaggcct tccagcagga tccatcgaac agcaggctcc caatcgagga tcatctttcc
     1741 gttctgttcc cttatcccga aaggggctgg acgggatccc ccctggccga agactcgagc
     1801 tccatggcag cctgaccctc gtaagagatg acatccctta tccccagtac ggctgcgacc
     1861 gcatcgaaca gccttccgca ggaggatgtg accggagcct tatccagggt tgaaagaatg
     1921 ctgccgaaaa ggtggctgtg ggtgggccat atctctcctg caagtctttc agcatcacct
     1981 gggcccaggg ctttctccat aagggccagg gcaaaacgcc aaggttcgag gactgccctg
     2041 tcgccgcctg gaaggggcgc ctccctgaag tgacccaccc gggtgaacga acttatgtcg
     2101 ccgagaagga actctccccc ccagatattc ctgtcgcttc ccagtcccgt cccgtccaaa
     2161 atgatcccca gaaccgttcc ttcgtgcccg ttctcgacaa gacatgccgc catgtgtgcg
     2221 tgatggtgct ggaccaggag ggtgtcctcc ggttttccca tcactttttg ggcgatgtcg
     2281 gtacatgcgt aggaggggtg caggtccgcg gccaaaaggt tgggtctcag tccgaaaagc
     2341 ctgataaaat gcctcagggc cttctcatag tagacggctg tatcgagc
//
LOCUS       DAOPKQ010000215         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_51813_length_2377_cov_3.770457, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000215 DAOPKQ010000000
VERSION     DAOPKQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51813_length_2377_cov_3.770457"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PK146_06860"
     CDS             complement(<1..391)
                     /locus_tag="PK146_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE91994.1"
                     /translation="MLSIRDLNVHYGGIHAIKGISLEVPQGKIVTLIGANGAGKSSTL
                     RTIAGIVKKSKGSIQLDGREISGFAPEEIVKSGIMMAPEGRKILPHLTVLENLMLGAF
                     SRSDSDGIARDLDWVYELFPRLKERTWQ"
     gene            complement(384..1172)
                     /locus_tag="PK146_06865"
     CDS             complement(384..1172)
                     /locus_tag="PK146_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE91995.1"
                     /translation="MPVSDFILETEALTMRFGGLVAVRDFSVRVPRGSIVGLIGPNGA
                     GKTTCFNVVTGFYKPTEGRILFRGEDVTGWAPHKVCSAGIARTFQNIRLFSTASVLEN
                     VMVGARVRQSTSWWHALLSLPSYWKEEREVRDKAMFLLESVGLQKLAGEESGGLPYGA
                     QRRLEIARALATDPSFLLLDEPAAGMNPNETQELMGFVKDIRSRFDLTVLLIEHDMKV
                     VMGICEHIWVLDYGVTIAEGKPEEIQSNPRVIEAYLGEEFIQDA"
     gene            complement(1172..2221)
                     /locus_tag="PK146_06870"
     CDS             complement(1172..2221)
                     /locus_tag="PK146_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE91996.1"
                     /translation="MIDPKLKRNYTLNGVAVLLLAGILYWAGGNLDGYKIQIMNLIAV
                     NIILALSLNLIYGFTGLFSLGHAGFMAIGAYTCSLLILPAAHKQAMFLLQAPMWPFSV
                     IHAPFLVAVVIAGLVAAFFGILIGLPVLRLGGDYLGIATLGFAEIIRVLANNMPRVTN
                     GALGLKGIPSYSNLWWNYGWCLLTLFVIIRLVRSNFGNTLKAIRDDEVAARAMGINTF
                     RYRLTSFTIGAFFAGVGGALLASLLTTIDPKMFLFILTFNVLMIVVAGGLGSITGTVL
                     AGVVITVLLEWLRFVENPISLGNMTIPGIPGMRMVVFSLALLFIILFRHQGIMGRREF
                     SWDWFFGLFRKRGEA"
     gene            complement(2218..>2377)
                     /locus_tag="PK146_06875"
     CDS             complement(2218..>2377)
                     /locus_tag="PK146_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE91997.1"
                     /translation="IGGLILGFMEIMIVAFMPALAGFRDAFAFVLLIIILLIMPTGLM
                     GEKLEDKV"
BASE COUNT          564 a          700 c          626 g          487 t
ORIGIN      
        1 tctgccaagt cctctccttg agcctcggga agagctcgta gacccagtca aggtccctgg
       61 ctatcccgtc ggagtcgctc ctgctgaagg cacccagcat aaggttctcc agaacggtca
      121 gatgggggag gatcttcctc ccctcagggg ccatcattat ccctgacttg acgatctcct
      181 ccggggcaaa ccccgatatc tccctcccat cgagctgaat ggagcccttg cttttcttca
      241 caatgccagc aatggtccgg agagtggagc tcttgccggc accattggcc cctatcagtg
      301 tgactatctt cccctgaggg acctcgaggg agataccctt tatggcgtgg atgccgccgt
      361 aatgaacatt gaggtctctg atactaagca tcttggatga actcctctcc caggtaggcc
      421 tcgatgaccc tggggttgga ctggatctcc tcgggcttgc cctcggctat ggtcaccccg
      481 taatcgagga cccagatatg ctcacagatg cccattacca ccttcatatc gtgctctata
      541 agcagcaccg taaggtcgaa ccgggaccgg atgtccttga cgaagcccat cagctcctgg
      601 gtctcgttgg ggttcatccc ggcagcgggc tcgtcgagaa gcaagaagga cgggtcagta
      661 gcaagggccc ttgctatctc cagcctccgc tgtgccccat agggcaggcc tccggactct
      721 tcgccggcga gcttctggag tcccacggac tcgagcagga acatggcctt gtcccttacc
      781 tccctctcct ccttccagta ggatggaaga cttagaaggg cgtgccacca ggaagtgctc
      841 tggcgaactc tcgccccaac cattacgttc tccaggacgc tggcagtgct gaagagcctt
      901 atgttctgga aggtccttgc tatgcctgca gagcacacct tgtgaggagc ccagcccgtt
      961 acgtcctccc ccctgaagag tatcctccct tcagtgggtt tgtagaaacc cgtcaccacg
     1021 ttgaaacagg tcgtctttcc ggctccgttg ggccctatta acccgacaat gcttcccctg
     1081 ggtacacgga ccgaaaaatc cctgactgcg acaagaccgc caaagcgcat cgtgagcgcc
     1141 tctgtctcta gaatgaaatc actgaccggc atcaggcttc acctctcttc cggaagaggc
     1201 cgaagaacca gtcccagctg aactcccgtc ttcccatgat accctggtgc ctgaaaagaa
     1261 taatgaagag aagggccagg gagaatacca ccattcgcat cccgggaata ccgggtatgg
     1321 tcatgttgcc cagcgaaatg gggttctcga cgaacctcag ccactcaagg agtaccgtga
     1381 taaccacccc cgcgaggact gtccccgtta tagaaccaag gccgcccgca acaactatca
     1441 tgagaacgtt gaaagtgaga atgaaaagga acatcttcgg gtctatggtc gtcagaagac
     1501 tggccagaag ggcccctccg accccggcga agaatgcacc gatggtaaag gaggtcagcc
     1561 ggtagcggaa ggtgtttatc cccatggccc tggcagcaac ttcatcgtcc cttatggcct
     1621 tgagggtatt cccgaagttg ctccttacaa ggcgaatgat cacgaacagc gtcagcagac
     1681 accatccgta attccaccag aggttcgagt atgacggtat tcccttcagc ccgagcgcac
     1741 cgttggtcac acggggcata ttgttcgcca gcactcttat gatctctgca aagccaaggg
     1801 tagctatccc cagataatcc ccaccgagcc tcagtacggg aagacctatc agtatcccga
     1861 agaaggccgc caccagcccg gcgatcacca cagccaccag gaagggagca tgaatgaccg
     1921 agaagggcca catcggagcc tggaggagga acatggcctg cttgtgtgcg gcaggcagaa
     1981 tgagaagaga gcaggtatag gccccgatgg ccatgaaacc ggcatgtccc agtgaaaata
     2041 accctgtgaa accgtagatg aggttaaggc tgagtgcaag aatgatattg acggcgatca
     2101 ggttcataat ctggatcttg tagccgtcaa ggttgccgcc ggcccagtag aggatgccgg
     2161 ccaggagaag taccgcaacg ccgttaagcg tgtaatttct cttgagttta gggtcgatca
     2221 tactttatcc tccagcttct cacccattaa tccggtaggc atgatgagaa gaatgatgat
     2281 cagaagaacg aaggcgaaag catccctgaa accggccaaa gcgggcataa aggctacgat
     2341 cattatctcc atgaatccca ggatcaggcc cccaata
//
LOCUS       DAOPKQ010000216         2364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_52196_length_2364_cov_4.587700, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000216 DAOPKQ010000000
VERSION     DAOPKQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52196_length_2364_cov_4.587700"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..746
                     /locus_tag="PK146_06880"
     CDS             303..746
                     /locus_tag="PK146_06880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPE91998.1"
                     /translation="MGPVITARIRVRYGETDRMGIVYYANYLTWFEIGRTEFCRSLSK
                     PYTLWEESGLNLPAVEAHVRYKNPLTYDETVLIETRIREVTSHSVSFSYKVRPEERSR
                     LAAEGWTRHAFCGADGRLLREPEPFYGWLKELAGSSLEEVVPREQ"
     gene            736..2334
                     /locus_tag="PK146_06885"
     CDS             736..2334
                     /locus_tag="PK146_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPE91999.1"
                     /translation="MSNKAILLGNEAIARGVIEAGCEVAAAYPGTPSSEILPAIAKWA
                     DELKTKTVVEWGANEKVAFEMAAAASFAGKRSCAVMKQVGLNVAADPFMSVAHFQLKG
                     GFLLIAADDPGPHSSQTEQDSRFFAMFAKIPCFDPSTAVEAKEMVFDAFDLSERHGVV
                     TMLRPGVRVDHCRQDVELKDVIDISRPAAFEKDPKRWVCLPARVRVNHPQLNAKREAI
                     REEFETKFSRYNYEVPAKKKARLGIIAGGVSFSIVTDILRSWGAEDVSILKIGTPHPL
                     PLKMVEDFISRHDDVLILEETYPVIEMQLNDRTRVKGRWNGWVPGAGELLPEIIEEIL
                     CKVLDRKAPEGASAEIKKAMEEMKITPRKPMLCPGCPHRASFFSIRQTLPKAIYPSDI
                     GCYTLGVNQGMVDAVVDMGASITQASGLYMAYKADGKEQPIVASIGDSTFFHMGLPGL
                     ASAVYNRHAFVLAILDNSLTAMTGGQSHPGIGTKLRDGEEGHKVDLEGAVKGCGVKWI
                     KTTEAYDISSSRAIVKEAWEEEMS"
BASE COUNT          540 a          629 c          735 g          460 t
ORIGIN      
        1 accaaggaag caggatactg gaaacaggag actgggaaaa ccttaaaggc gtaaacggtg
       61 aacggtgaac gggaagggca agggcggatc ctcagaaaga aaggttttct ctgtgtggct
      121 tcaccctctg cgacctctgc ggtgagaaag atttgggtct tgcccttgcc cctcacaaag
      181 aaaggtttcc tctgtattcc tgcgtcctct gtggtgaaga agcatttaag gcgtgaacgg
      241 gatttgtttt tgtaccgtta tggaatatac ttttcaaagt gttcaacgaa acgggggaga
      301 tcatgggacc tgtgatcaca gcgcgtataa gggtgcgata cggtgagacc gaccggatgg
      361 ggatagtcta ttacgccaac tatctcacct ggttcgagat aggcaggacg gagttctgcc
      421 gttcactttc gaaaccgtac actctctggg aggagtcagg tctgaacctt cccgccgtgg
      481 aggcccatgt ccgctacaag aaccccctga cgtacgatga gacagttctt atagagacga
      541 gaatacggga ggtgacttcc cacagcgttt ccttctccta caaggtaaga ccagaggagc
      601 gtagccgcct tgcggcggag ggttggacca ggcacgcgtt ctgcggcgcc gatggccgac
      661 ttctccggga gcccgagcct ttctacggct ggctgaaaga actggccgga tcttcattgg
      721 aagaggtggt accgcgtgag caataaggca attctgcttg gtaacgaggc aattgcccgg
      781 ggagtgatcg aggccgggtg cgaggtagca gcagcctatc cgggaacgcc gtcatcggag
      841 atcctcccgg cgatagcaaa atgggcggac gaactcaaga cgaaaacagt tgtggagtgg
      901 ggagccaacg agaaggtcgc cttcgagatg gcggcagcgg cttcctttgc gggaaagcga
      961 tcctgcgccg taatgaaaca ggtgggtcta aacgtcgccg cggatccttt catgagtgtg
     1021 gcgcactttc agttgaaggg gggcttcctc ctcatcgccg cggacgatcc ggggcctcac
     1081 agttcacaga cagagcagga cagccgattc ttcgccatgt tcgcaaagat accctgcttt
     1141 gatccatcca cggcggtcga ggccaaggag atggtcttcg acgccttcga cctctcggaa
     1201 aggcacggag tggttaccat gcttcgcccc ggagtgaggg tggatcactg ccgccaggat
     1261 gtggaactga aggatgttat cgacatttcc cgcccggccg cctttgaaaa ggatccgaaa
     1321 cgctgggtct gccttccggc cagggtaagg gtgaaccatc cgcagctgaa cgccaagagg
     1381 gaagccatcc gcgaggagtt cgagacgaag ttctcccggt acaactacga ggtcccggca
     1441 aagaagaagg cgaggctggg gatcatagcc gggggcgtat ccttctcgat agttacggat
     1501 atcctcagat cctggggagc ggaggacgta tccatcctca agatcggcac gccgcacccg
     1561 cttcctctca agatggtaga ggatttcatc tccaggcacg atgatgtgct gatcctcgag
     1621 gagacctacc cggtgatcga gatgcagctg aacgacagga ccagggtcaa ggggcgctgg
     1681 aacggctggg tccccggcgc tggagaactg cttccggaga tcatcgagga gatcctctgc
     1741 aaggtcctgg acaggaaggc acccgagggc gcgtcggcag agatcaaaaa ggccatggag
     1801 gagatgaaga tcaccccccg caagccgatg ctctgcccgg gatgccccca cagggcaagt
     1861 ttcttctcca tcaggcagac cctccccaag gcgatatacc cgtccgacat agggtgctat
     1921 accctggggg tcaaccaggg aatggtggac gcggtggtgg atatgggcgc ttcgatcaca
     1981 caggcttcgg gtctgtatat ggcctacaag gcggacggca aggagcagcc tattgttgcc
     2041 tccatcggag actctacctt cttccatatg ggtcttccgg gcctggccag cgccgtttac
     2101 aacaggcacg cctttgttct ggccatcctg gacaacagcc tcaccgccat gaccggcggc
     2161 cagtcccacc cgggcatagg caccaaactg agagatggcg aggaagggca caaggtcgac
     2221 ctggaagggg ccgtcaaggg gtgtggggtc aagtggatca aaaccacaga ggcctacgac
     2281 atctcttcct ccagggccat cgtaaaggag gcctgggagg aagagatgtc gtaggcctct
     2341 gtggttttga tccacttgac ccca
//
LOCUS       DAOPKQ010000217         2354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_52514_length_2354_cov_4.204437, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000217 DAOPKQ010000000
VERSION     DAOPKQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52514_length_2354_cov_4.204437"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PK146_06890"
     CDS             complement(<1..1224)
                     /locus_tag="PK146_06890"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPE92000.1"
                     /translation="MAERYIIEVGGRPLTFETGKLAKQANGSVQVTMGETSILVTACI
                     ADKAREGLDFLPLLVDYEERFYSAGKIPGGFIKREGRPSETATLTSRIIDRSIRSLFP
                     GHIRNDIHVVATTLSVDQSNPPNIVAINGASAALMLSGIPWGGPVGAVRIGLVDGELV
                     VNPTEDMMKESALDLVVSGHAGGITMVEAGAREIPEQLMVDALDLAQREITAITEKIG
                     EMAKAFGKPVMELPEPPSFPEIDDWIGKELYGDIHQAVQIHDKKPRGEAIDSATLKAL
                     DNLLEKYPESESYIKTAVEEMVKKAMRSLIVEESKRADGRAMDEVRPITCEVGILPRV
                     HGSALFTRGETQALVTTTLGMVGEDDQLVDGLKHDEPSKRFMLHYNFPPFSVGEVRPM
                     RGPGRREIGHGALAER"
     gene            complement(1365..1631)
                     /gene="rpsO"
                     /locus_tag="PK146_06895"
     CDS             complement(1365..1631)
                     /gene="rpsO"
                     /locus_tag="PK146_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPE92001.1"
                     /translation="MLEKEKKQGIIEEFKTHNADTGSPEVQVAILTTRIRDLTEHLKI
                     HKKDHHSQRGLLKMVGRRRRLLQYLRNKDFGRYSDLIQKLGLRH"
     gene            1859..>2354
                     /locus_tag="PK146_06900"
     CDS             1859..>2354
                     /locus_tag="PK146_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE92002.1"
                     /translation="MKILIVEDNRDLVQVLSEGFNDLNYSVESAFDGIEGLNKIRNSD
                     YDCIILDIMLPEMDGYEVVERAREEGKDTPVIMLTAKDSIEDRVEGLNRGADDYKGVD
                     IVLSSPGGKVSAEYSPKAAKAGAVVIDNTSHFRMEPDVPLVVPEVNPQDVALHTKQGI
                     IANPN"
BASE COUNT          502 a          675 c          607 g          570 t
ORIGIN      
        1 cctctctgcc agtgccccgt gccctatctc cctgcgcccg ggccccctca tgggcctgac
       61 ttctccgacg gagaagggag ggaagttgta gtggagcatg aagcgcttcg acggctcgtc
      121 gtgcttgagg ccgtccacga gctggtcgtc ctctcctacc atccccaggg tggtcgtcac
      181 cagcgcctgg gtctcacccc tggtaaagag agctgaaccg tgtacccttg ggaggatccc
      241 cacttcacag gtgatgggcc ttacctcatc catggccctt ccgtctgccc ttttgctctc
      301 ttcgacgatg agggacctca tggccttctt gaccatctcc tcgacggcag tcttgatgta
      361 ggactccgat tcggggtact tctcaagcag gttatccagg gccttcaaag tggcggagtc
      421 gatcgcctct ccccgcggct tcttgtcatg gatctgcacg gcctggtgga tatctccgta
      481 gagttccttg ccgatccagt catcgatctc gggaaacgat ggcggttccg gtagttccat
      541 tacgggtttc ccgaaagcct tggccatctc cccgatcttt tccgtaatgg cggttatctc
      601 ccgctgggcc agatcaaggg catcgaccat taactgctcc gggatctccc gggcgccagc
      661 ctcgaccatg gttataccac cggcatgtcc gcttacgaca aggtcaaggg cgctctcttt
      721 catcatgtct tccgtaggat tgacaacaag ctcgccatca acaagaccta tccttactgc
      781 tcccacaggc cctccccacg gaatccccga gagcattagc gccgcagaag caccgttgat
      841 ggccactatg ttcggcgggt tcgactggtc cactgaaagg gtggtcgcaa caacatggat
      901 gtcgttgcgg atgtgccccg ggaagagcga ccttatcgag cggtcaatta tcctggacgt
      961 cagtgttgcc gtctcggagg gtctcccctc cctctttatg aagcccccgg ggatcttgcc
     1021 ggccgaatag aatcgttctt cgtaatccac aagcaagggc agaaagtcca gcccttccct
     1081 tgccttgtca gcaatgcaag ccgtcacaag gatcgatgtc tcccccattg tgacctgaac
     1141 cgaaccgttt gcctgtttgg caagctttcc cgtttcgaag gtcaggggac gccccccgac
     1201 ttcaataatg tatctttccg ccataattct gtttcctcct catatttttc ccgtcttttt
     1261 tcccgttaaa ggataacaca agcggacaaa aaagaacgag ggcaagccgc cctcgttcca
     1321 aaacatgccg tgtcctgcct tttggtcttc cagggattat gcgatcagtg cctcagaccg
     1381 agcttctgga tgagatcgct ataacgtccg aaatccttgt tcctgaggta ctgcagaagc
     1441 cttctcctgc gccctaccat cttgaggaga cccctctggg agtggtggtc cttcttatgg
     1501 atcttcagat gttccgtcag atccctgatc cgggtggtca ggatcgctac ctggacctcc
     1561 ggtgaacccg tgtcggcgtt atgggtcttg aactcctcga tgatgccctg tttcttctcc
     1621 ttttcaagca ctgccttcac ctcttggtgt atattcccgg aaccaagtag tgcgcatatc
     1681 cgcagaacct agtatcgggt gcaggaatta tatcaccata tagtgtgccg ggcaaacaaa
     1741 tccaattatg gaaaaaatat aaggttccgc tcattctgca ctcgacattt tcttaattat
     1801 atgctttcat tcttaatata gactctacag aatctgatcg aaaggaagga taaactccat
     1861 gaagatcctc atcgtagagg ataacaggga ccttgtgcag gtcctctcgg aagggttcaa
     1921 cgaccttaac tattcagtgg aaagcgcctt tgacggtatt gaaggactga acaagatccg
     1981 aaacagcgac tatgactgca tcattctgga catcatgcta cctgagatgg atggctatga
     2041 agttgtagag agggcaaggg aagaggggaa ggacaccccg gtgataatgc tcaccgccaa
     2101 ggactccata gaggacaggg tggaaggcct caaccgggga gccgacgact acaagggggt
     2161 cgatattgtg ctgtcctctc ccgggggaaa ggtatcggcg gaatattcgc ccaaagcggc
     2221 gaaggccggc gccgtcgtga tcgacaatac cagccatttc cgcatggagc cggatgtgcc
     2281 tttggttgtg ccggaggtga acccgcagga tgtggcgctg catacaaaac agggcattat
     2341 cgccaacccg aatt
//
LOCUS       DAOPKQ010000218         2352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_52573_length_2352_cov_4.004789, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000218 DAOPKQ010000000
VERSION     DAOPKQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52573_length_2352_cov_4.004789"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PK146_06905"
     CDS             <1..1120
                     /locus_tag="PK146_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPE92003.1"
                     /translation="SLIDCISDSPSADRRYGRKTPERVEIPKPDAYRGIPRRYWHFGL
                     TREELASRIISQPRPDLVLVTSSMTYWYKGVFWCIEQLRKILPGVPVILGGVYPVLCP
                     DHALLSGADEIQTVPMPLPGSMPAMDLYRRLSYGVAVTSTGCPRRCSYCASSLLWPSF
                     IQGDPLNLMAEIDLQVDLGAADLAFYDDALLIGKEDHFYPICEELTARHPGLRLHTPN
                     GLHVREIDHTCASTLFRTGFRTIRLSLEGIDTANRQAGARKTSPGDYSAAVANLLEAG
                     FRPDQVETYVLAGLPGQRACDVASSIGFVRSMGARAKLAQFSPIPGTPLFDEVVRTHP
                     EVQEEPLLQNNTFYSPCVSGEITPEELQSLKDMARTPG"
     gene            1189..2337
                     /locus_tag="PK146_06910"
     CDS             1189..2337
                     /locus_tag="PK146_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPE92004.1"
                     /translation="MNSFQALTAKFFTGNFAIMAFGDLYFILALYLQDMGITDPRTLG
                     WILGIYFAASTVTRPFSGFIIEKFSFRRVMLTASLLCLASGAGVALAGTSVSLILVFR
                     ALTGFSSSLFLVGLTTYQALVVPEEIRGSSFTLASAGTIAPLIAILPVAEWLLRNGHK
                     FIYIWSPLLPAILCLTVAAAIPPADDLALKKGNWGTYSEIFRIKASRTLLTSVVLFAM
                     TDAVIISMAGLAMERGVLASSFISSQAFTGLMVRLFGYRLMDRLPRSRLAALCFSVTA
                     FGSVAITFVNSNGGMIFWGIVYGIAMGYGFPLHLSLIGDAVPERLRPKGTSLVWFVMA
                     GCYFISPVLTGFIARWLNFSWAFRIICGTIILCAPLMHREFKRSFERA"
BASE COUNT          449 a          759 c          620 g          524 t
ORIGIN      
        1 aagcctcatc gactgcataa gtgattcgcc ctccgccgac aggagatacg gaagaaaaac
       61 ccccgaaagg gttgagatcc caaaaccgga cgcctaccgg gggatcccga gacgttactg
      121 gcacttcgga cttacccggg aggagctggc ctcaaggatc atctcacaac cccgccctga
      181 cctggtcctg gtcacatcct cgatgaccta ctggtacaag ggtgtcttct ggtgcataga
      241 gcagctacgc aagatccttc ccggcgtacc ggtgatcctc gggggggtct atccggtcct
      301 ctgcccggac cacgccctcc tctccggcgc ggatgagatc cagacagtac ctatgccttt
      361 gccaggatcc atgccggcaa tggaccttta ccgccggctg tcctacggcg tagccgtaac
      421 atccacgggg tgtccgcgaa gatgctccta ctgcgcctct tcccttctgt ggccttcgtt
      481 catccaggga gatcccctga acctgatggc agagatagac ctccaggtcg accttggggc
      541 cgccgacctg gctttctacg acgatgccct tcttatcgga aaggaagatc atttctaccc
      601 gatctgcgaa gaactaaccg cccgtcaccc cggactcagg ctccataccc ctaacgggct
      661 tcacgtacgt gagatcgatc acacctgtgc atccactctt ttccgtaccg ggttcagaac
      721 catccgcctc agcctggagg gcatcgacac cgccaatcgc caggcggggg cccggaagac
      781 ctcccccggg gattactcgg cagcagtggc caacctcctg gaggctggtt tcagaccaga
      841 ccaggtggag acctatgtcc tggcggggct cccgggccag agagcatgcg atgtcgcaag
      901 ttcgatcggt ttcgtccgtt ccatgggggc aagggcgaaa ctggcccagt tctcacccat
      961 ccccgggacc cctctcttcg acgaggtcgt ccggacgcac cccgaggtcc aggaagaacc
     1021 gctccttcag aacaacacct tctactcccc ctgcgtgtcc ggagagatca cacccgagga
     1081 gcttcagtcc ctcaaggata tggcaagaac ccctggttga cactttttcg acctcttgtg
     1141 ctaaactgct tcagatccgt ttgcatgcat gaagaaggag ccttgttcat gaactcgttt
     1201 caagcattga ctgccaaatt ctttaccggc aactttgcga taatggcctt cggagatctt
     1261 tacttcatcc tcgccctgta cctgcaggat atggggatca cagaccccag gaccctgggg
     1321 tggatcctgg ggatctattt tgctgcttca acggtcaccc ggccgttctc gggcttcata
     1381 attgaaaaat tctccttcag gcgggtaatg ctgacggcat cgctgctatg ccttgccagc
     1441 ggagccggcg ttgcccttgc cggtacctcg gtctccctga tcctggtctt ccgggccctg
     1501 acgggtttct catcgagcct tttcctggtg gggcttacca cctaccaggc ccttgtggtc
     1561 cccgaagaga taaggggttc ttccttcacc cttgccagcg ccggtaccat cgcccccctc
     1621 atcgcgatcc tccccgtcgc ggagtggctt ctgaggaacg ggcacaagtt catctacata
     1681 tggtcgcccc tcctcccggc cattctctgc ctgaccgtcg ctgccgccat accgcccgcc
     1741 gatgacctgg ccctgaaaaa gggcaactgg gggacctact cggagatatt tcgcataaaa
     1801 gcatcgagga cacttctaac gtcggtagtc ctcttcgcca tgacagacgc ggtcataatc
     1861 tccatggccg gccttgccat ggagaggggc gtgctcgcat cctcgttcat ctcgtcccag
     1921 gctttcaccg gccttatggt caggctgttc ggctacaggc ttatggaccg cctgcccaga
     1981 agcaggctgg ccgccctgtg cttctccgta acggcattcg gctccgtggc gatcaccttc
     2041 gtcaactcca acggggggat gatcttctgg ggtattgtct acggaatagc aatggggtac
     2101 gggttccccc tccacctgtc tcttatcggc gacgccgtcc cggaaaggct cagacccaag
     2161 gggacctccc tggtatggtt cgtaatggcg ggatgctact tcatctcacc cgttctgaca
     2221 ggcttcatcg caagatggct gaacttctca tgggccttca ggataatatg cggcacgatc
     2281 atcctctgtg cccccctgat gcacagggag ttcaagaggt cctttgagag ggcatgacca
     2341 tgacattctt ta
//
LOCUS       DAOPKQ010000219         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_53499_length_2324_cov_4.422653, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000219 DAOPKQ010000000
VERSION     DAOPKQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53499_length_2324_cov_4.422653"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PK146_06915"
     CDS             complement(<1..1057)
                     /locus_tag="PK146_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPE92005.1"
                     /translation="MKVMDNKEGRAMDRTFDRGATERLLSDLVRIPSPYFEEKEIMGF
                     ARDWLERRGVPAEFHHFHEKKVYDYKGINVIGTLEGGLPGPKVILNGHLDTVKLCSGW
                     TRDPFGASIEGNRMFGLGACDMKSGCATIMLALQEFVKDASPFRGSIHYSLVCDEEGP
                     FGLGTDALILDGKIPPDADVALITEPSSAFADVGFPSICLGARGGWNYSVKVRGRSAH
                     GAQPEEGINAVSEAAKILLELEKSELEPHEKLGPGSICCVEFHGGGAALSVPDEASFN
                     VFRHVVVGETRETVIREAEEAVRRACIKGKADIRFRVAPHDECDGFPSYVTPEDHEMV
                     RQMVSSIREATGREPAFS"
     gene            1277..>2324
                     /locus_tag="PK146_06920"
     CDS             1277..>2324
                     /locus_tag="PK146_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE92006.1"
                     /translation="MTMPRGNVAVLIENAGIWDTNRSRPFTGSLLIAGDRIVSAGEAE
                     QVTDHPASRNAFRIDAAGSSVIPGMTDSHLHLTALSRQRSALSLFEASSKEELLEMIR
                     RRASELGPGDWVYGVRFDNSRWADTTLPTLEELDSLGIPNPVLLLRVCAHLHVVNSRA
                     LQEAGLAAEDISGAGAVRDKKGCFTGTLRESFAQPVVQAMKRSSTGGTREAVALREAM
                     LELASQGITCIHTCSASSYGLEENLEAFHYLHDKGELPIRIRLYSDVAIPGWTKSGEG
                     DDWLCYGGKKFFLDGSLGGRTAAMTFPYEDDPSTRGMLNMDTGEFIEQVREEHLAGNQ
                     VKVHAIGDAAIDQFI"
BASE COUNT          537 a          672 c          604 g          511 t
ORIGIN      
        1 aactgaaggc aggctcacgg cctgtggcct ctcttatgga ggagaccatc tgccttacca
       61 tttcgtgatc ctcaggggta acataggaag gaaacccgtc acactcatca tggggggcga
      121 ccctgaacct gatatctgcc ttacccttaa tgcaggcccg cctgactgcc tcttctgcct
      181 cccttatcac cgtctccctg gtctctccca ccactacatg acgaaagacg ttaaaagatg
      241 cctcatccgg gacggaaaga gccgcccctc cgccatggaa ttccacacag caaatggaac
      301 ccggtccaag cttttcatgg ggttccagtt cgctcttctc taactccaac aggatctttg
      361 ccgcttcgct aacggcattt atcccctcct ctggctgtgc accgtgggcg gatctcccac
      421 ggacctttac agagtagttc catccacccc ttgctcccag gcagatgctg ggaaacccga
      481 catccgcaaa ggcgctgctc ggctctgtta tcaatgctac atcggcatca gggggtatct
      541 tcccatccag gatcagggca tcggtcccga ggccgaaggg cccttcttca tcacagacaa
      601 ggctgtaatg gatgcttccc ctgaaggggg aggcatcttt aacgaactcc tgaagggcga
      661 gcattatggt ggcgcatccg cttttcatat cgcaggcgcc gagaccgaac atcctgttcc
      721 cctcgatcga agcgccgaag gggtccctgg tccaaccgct gcaaagtttt accgtatcaa
      781 gatgtccatt caggatcacc tttggaccgg ggagcccccc ctccagggtg ccgataacat
      841 tgataccctt gtagtcgtaa actttctttt catggaaatg atggaattca gcggggacac
      901 cgcgtctctc taaccaatcc cgggcaaaac ccatgatctc cttctcttcg aaataggggc
      961 tcggtatcct gaccaggtct gaaaggagcc tctcagtcgc acccctgtca aatgttctat
     1021 ccattgccct gccttccttg ttgtccataa ctttcatgtc gggaccactc cccgcaaaca
     1081 aaatattttt aattaaagaa ggaaacagat agtttgacat ttcaatagat ataatctacg
     1141 ttaccagatt attacaatgc gtcaaattgt atgccttaag ggaacaacgg tttcatggac
     1201 tgtttaaggg ttgatgcttt ttaccgctgc ctttatcctc aattcgagat ctctttccat
     1261 ggaggaggga gggtggatga caatgccaag agggaacgta gccgttttga tcgagaatgc
     1321 cgggatctgg gacaccaaca gaagtagacc cttcacgggt tcgctgctga tagcaggaga
     1381 ccggatcgtt tcggctggag aagcggagca agtaactgac catcctgctt cccgtaatgc
     1441 tttccgaata gacgcagccg gctcctcggt tataccaggc atgacggact cgcaccttca
     1501 cctgacggcc ctttcgaggc agagatcggc cctgtcgctg tttgaggcat catcgaaaga
     1561 agagctcctg gagatgatcc gcagaagggc ctcggaactg ggccccgggg actgggtata
     1621 cggggtccgt ttcgacaact cacgatgggc cgatactacc ctgccgaccc tggaagaact
     1681 ggattccctt ggcatcccca accctgtcct tcttctcagg gtctgcgccc accttcacgt
     1741 ggtcaactcc agggcgctcc aggaggcggg gcttgcagca gaagacattt ccggtgctgg
     1801 cgcggtcaga gacaaaaaag gttgcttcac cgggaccctc cgtgaaagct ttgcccagcc
     1861 agtggtccag gcaatgaaaa gaagctccac agggggcacc agggaggccg tcgctctcag
     1921 ggaggccatg ctggaactgg catcacaggg gatcacctgc atccatacct gcagcgcttc
     1981 atcctacgga ctggaggaga acctggaagc cttccattac ctccacgata aaggtgaact
     2041 tcccatcaga atacgcctgt acagcgacgt ggccatccct ggatggacaa aaagtggcga
     2101 aggcgacgat tggctttgct acggggggaa gaaattcttc ctcgacggct cactgggagg
     2161 acgtacggcg gcgatgacat ttccctacga agatgatcca tcgaccaggg ggatgctcaa
     2221 catggacact ggggagttca tcgaacaggt cagggaggaa cacctggccg ggaaccaggt
     2281 gaaggtccac gccatcggcg acgcggccat cgaccagttc atca
//
LOCUS       DAOPKQ010000220         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_53605_length_2321_cov_3.679170, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000220 DAOPKQ010000000
VERSION     DAOPKQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53605_length_2321_cov_3.679170"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="lpxK"
                     /locus_tag="PK146_06925"
     CDS             complement(<1..533)
                     /gene="lpxK"
                     /locus_tag="PK146_06925"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPE92007.1"
                     /translation="MGALLTSYFRHIRGEEEGFPWTLLSPLGWLAGTCSRARNFSYDH
                     GLSISREMPVPVISVGNITHGGTNKTPFVEMLARMFMDAGLRPGIVMRGYGRKEKDCL
                     LAQECEPRRDLLGDEALLLSGRLGGVPIAVSADRAGGVLRLAEEGVDLAIADDGFQHR
                     KMGRDVDVALIDATCPFG"
     gene            complement(514..2289)
                     /locus_tag="PK146_06930"
     CDS             complement(514..2289)
                     /locus_tag="PK146_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE92008.1"
                     /translation="MKPLKKRQETGDRVYPRLLVYARPYVHRILGALACMVLSSACAV
                     VVPWLLKNIVDDVLISRNMVMLNIIAVGIVIIYTAKSVFYYGHQYLMSWVGQRVVLDL
                     RLRLYDHMQRMSFRYLYGHRVGEMLSRITNDVNILQNMITNVFIDIVVQGLSFVGIIG
                     FLLYINWKLSLLSFLVLPLAALVLDVASKRLRLVGHDIQQQLAGLSAIAAEALSAIRI
                     VRLFATEEQEFGRFESQSNAHFRALMRGVQTNAALTGIVEVILISALAIILWFGGRLV
                     VSGELTPGELIAFLGYLALLVQPVRVFSRVVAQMQQGLAAADRVFEILDIESEVQPPK
                     HPVRADDISGEITFRDVSFAYNEGAWVLENVSFHISPGEKIAIVGPTGAGKSTIADLI
                     TRFYDPQEGAVLIDGMDLKRLDLKGLRRKIGVVPQDPVLLKGSIAFNIAYGCEDLSKD
                     DIESAARVAGIYGFVMSLPDGFQTEVGERGVTLSGGQRQRIAIARAIVRDPRILIMDE
                     ATSSLDAAVEQEIQEAMKKAMSGRTSIVIAHRLATVREADRILVIDRGRIVEEGTHEV
                     LKEAGGLYSRLCSLQFGDHNGSPAH"
BASE COUNT          483 a          704 c          647 g          487 t
ORIGIN      
        1 ccgaaggggc aggtcgcgtc aatgagcgca acatcaacgt ccctgcccat tttcctgtgc
       61 tggaacccgt cgtcggctat ggcaaggtct actccctctt cagccagccg cagtacccca
      121 cctgcacggt ctgcagagac cgctatgggg accccgccaa gccttcccga gagaagaagg
      181 gcctcgtctc ccaggaggtc ccttctcggt tcgcactcct gggccaggag gcagtccttc
      241 tccttcctcc cgtagccgcg cataactatc cctggccgga gaccggcatc catgaacatc
      301 ctggccagca tttccacgaa gggggtcttg ttggtgccac cgtgggtgat attcccgacg
      361 ctgatcaccg ggacaggcat ctcgcgggag atagaaaggc cgtgatcgta agagaaattc
      421 ctggccctgg aacaagtccc tgcgagccac cccagtggag aaaggagggt ccaggggaac
      481 ccctcctctt cgcccctgat gtgcctgaaa tagctagtga gcagggctcc cattgtgatc
      541 cccgaactgg agggaacaga gcctggagta gagaccacca gcttctttca ggacctcgtg
      601 ggtaccctct tcgacgatcc tgcccctgtc tattaccagt atccggtcgg cctcgcggac
      661 ggtggcaagc ctgtgggcga tgactattga ggtcctccct gacatggcct ttttcatcgc
      721 ctcctggatc tcctgctcca cggcggcgtc tagagatgaa gtggcctcgt ccattatgag
      781 tatcctggga tcccggacta tggcccttgc gatggcgatc ctctgtcgct gacctccgct
      841 cagggtgaca ccacgctccc cgacctcggt ctggaaaccg tccggcaggg acatgacgaa
      901 accgtatatt cccgcgaccc gggcagcgct ctcgatatca tctttggaaa ggtcctcgca
      961 accgtaggca atgttgaagg cgatgctgcc tttaaggaga accgggtcct gggggaccac
     1021 tccgatcttc ctcctcaacc ccttcaggtc aagccttttc agatccatcc catcgataag
     1081 gacggctccc tcctgcgggt cgtaaaagcg ggtgataagg tcggcgatcg tggacttgcc
     1141 cgcgccggtg ggccctacaa tggcgatctt ttcgcctgga gagatgtgga aagagacgtt
     1201 ctcaagcacc catgcccctt cgttgtaggc gaaggacaca tcccggaagg tgatctcccc
     1261 gcttatgtca tctgccctca ccgggtgttt cggaggctgg acctcgctct cgatatccag
     1321 tatctcgaag accctgtccg ctgcggcaag cccctgctgc atctgggcaa ctaccctgga
     1381 aaagaccctg acgggctgga ccagaagagc caggtagccc aggaaagcga tcagttcacc
     1441 gggtgtgagt tctccggaaa caaccagacg acctccgaac cagaggatga ttgccagtgc
     1501 cgatatgaga atgacttcca ctatcccggt aagggcggcg ttggtctgca cccccctcat
     1561 gagggccctg aaatgggcgt tgctctggga ctcgaagcgg ccgaactcct gctcctccgt
     1621 ggcgaaaagc cggactatcc tgatagccga aagagcctcg gcggcgatgg ctgaaagccc
     1681 cgccagctgc tgttggatgt cgtggccgac cagcctgagc ctcttggagg ctacgtccag
     1741 cacaagtgcc gccaatggga gaacaaggaa ggaaaggagt gagagcttcc agttgatgta
     1801 gagcaggaag cctattatcc cgacgaaact cagcccctgg acgacgatgt ctatgaagac
     1861 gtttgtgatc atattctgca ggatattgac gtcgttggtt atcctggaaa gcatctcacc
     1921 gaccctgtga ccgtagaggt atcggaatga cattctctgc atgtggtcgt agagccggag
     1981 cctgaggtct aatacgaccc gctggcccac ccagctcatc aggtactgat ggccgtagta
     2041 gaaaacgctc ttggccgtgt agatgatcac tataccgaca gcgatgatgt tcagcatgac
     2101 catgttcctg ctgatcagga cgtcgtcgac tatattcttg aggagccacg ggaccaccac
     2161 tgcacaggcg gacgacagca ccatgcaggc cagggcgccc aggatacggt ggacgtaggg
     2221 tcttgcatag accagaagcc tcgggtatac cctgtcccct gtttcctgcc ttttcttcaa
     2281 aggtttcatc ccaccaactc tgatatttgc tctgctatcc t
//
LOCUS       DAOPKQ010000221         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_54323_length_2300_cov_5.976392, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000221 DAOPKQ010000000
VERSION     DAOPKQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54323_length_2300_cov_5.976392"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..98
                     /locus_tag="PK146_06935"
     tRNA            13..98
                     /locus_tag="PK146_06935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Leu,seq:cag)
     gene            270..>2300
                     /gene="rpoB"
                     /locus_tag="PK146_06940"
     CDS             270..>2300
                     /gene="rpoB"
                     /locus_tag="PK146_06940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPE92009.1"
                     /translation="MAEFVPVNSKRQRLTFGRTHDLVEIPDMVAIQRDSFRWFFQFNE
                     KGKLMDAGERSTQGLQELFDEVFPIESYDGSFALEFVDYFIDPPQITQEEASQRDLSW
                     SRSVKATIRLVNRKTEEIKEEEVYLGDFPMMTERGTFIINGTERVVVSQLARSAGVYF
                     SSEESIPGQERYAAKVIPDRGAWLEFDMTPGEVLSVNIDNRKKLPVTVLLKAFGVENN
                     DEILKLFGAVEEQIDLVEDEVRGRLVAETISDESGKSVVKKNERITKDILEKLWEMGR
                     TRVWVWKTDSAIEATMERDPTRQRDEAVMEMFRKLRPNEPARMENAKEYINGLFFDSR
                     RYNLGRVGRYKLNRRLGLEIPESRRLLLVEDVTQIVRKLMELRDGNERTDDIDHLGNR
                     RVRAVGELLQNQVRIGLLRMERIARERMTTIPDLTGATARDLINVRPISAALREFFGS
                     GQLSQFMDQTNPLAELTHRRRLSALGPGGLSRERAGFEARDVHYTHYGRVCPIETPEG
                     PNIGLVTSLSIYSRINEYGFLQAPKRKVVKGKVTDEVVFLGADEEDEFYIGRANTPLN
                     PDRTFSEKEIYVRDRGDIAVVEPEKVDFLDISPKQIVSASTALIPFLEHDDANRALMG
                     SNMQRQAVPLINGKAPVIGTGIEARVARDSGSCVVARSSGTVTRVDSTSIEIQ"
BASE COUNT          521 a          660 c          709 g          410 t
ORIGIN      
        1 atactggacg aaggggaagt ggcggaacgg tagacgcgct ggcttcagga gccagtgtcc
       61 ggaagggtgt gggggttcga atcccccctt ccccaccata aagttaaatc cgacccattg
      121 aaggatgggg tgaaacggtg gtcgcggtaa gagaaaaccg aagggttgcg ctgtgcctat
      181 tgacacagcg caacccttta gttatccttt gagtttgtca catcttgttc ctccaaatcc
      241 cctagagaca gggaggttat gtttctccca tggctgaatt cgttcccgtc aacagcaagc
      301 ggcagcgcct caccttcggt cgcacccatg accttgtcga gatcccggac atggtggcga
      361 tccagcgaga ctccttcaga tggttcttcc agttcaacga gaagggcaaa ctgatggatg
      421 ccggggagag gtccacccag gggctccagg agctcttcga tgaggtgttc cccatcgaga
      481 gctacgacgg ctcatttgcc ctcgagttcg tcgactactt catcgacccc ccccagataa
      541 cccaggagga ggcatcccag agggacctct cctggtccag gtcggtaaag gctaccatcc
      601 gcctggtgaa ccggaagaca gaggagatca aggaagagga ggtctacctg ggggatttcc
      661 ccatgatgac cgagaggggc acttttatca tcaacggtac cgagagggtt gtcgttagcc
      721 agcttgcccg ctcggcaggt gtctacttca gctccgagga gtccatcccc ggacaggagc
      781 gttacgctgc caaggtcatc cccgacaggg gggcatggct agagttcgac atgaccccgg
      841 gtgaggtcct ttcggtgaac atcgacaaca ggaagaagct acccgtgacg gtgcttttga
      901 aggccttcgg tgtcgagaac aacgacgaga tcctcaaact cttcggtgct gtcgaggagc
      961 agatcgacct ggtcgaggac gaggtcaggg ggagacttgt cgccgagacc atttccgacg
     1021 agtccggcaa gagcgtcgtc aagaagaatg aaaggatcac caaggatatc ctcgagaagt
     1081 tgtgggaaat gggtaggaca agggtctggg tctggaagac cgactcagcc atcgaggcga
     1141 ccatggagcg cgaccccacc aggcaaaggg acgaggccgt tatggagatg ttccggaagc
     1201 tccgtcccaa cgagccggcc aggatggaga acgccaagga gtacatcaac gggcttttct
     1261 tcgattcccg caggtataac cttggccgtg tcggccgcta caagctgaac cggcggcttg
     1321 gcctggagat ccccgagtcg aggcgcctgc tcctggtaga ggatgtgacc cagatagtaa
     1381 ggaaactcat ggaactgagg gatggcaacg agcgcaccga cgacatcgac catctaggca
     1441 accggagggt ccgcgccgtg ggtgagctcc tccagaacca ggtgcgtata ggcctcctca
     1501 ggatggagag gatcgcccgc gagaggatga ccaccatccc cgacctgacc ggggccactg
     1561 ccagggatct catcaacgta cgccccatat cggcggcgct cagggagttc ttcggctccg
     1621 gccagctctc ccagttcatg gaccagacca atcccctggc ggagctgacc caccggagga
     1681 gactctctgc cctggggccg ggaggcctga gcagggagag ggcgggcttc gaggccagag
     1741 acgttcacta cacccactac ggaagggtat gccccatcga gacccctgaa ggtcccaaca
     1801 tagggctggt gacctccctg tccatctatt cgagaataaa cgagtacggg ttcctccagg
     1861 ccccgaagag gaaagtggtc aaaggcaagg tcaccgacga ggtggtcttc ctgggcgccg
     1921 acgaggagga cgaattctat atcggaaggg ccaacacccc actcaacccc gacaggacct
     1981 tctccgagaa ggagatctac gtcagggaca ggggagacat cgccgtggta gagccggaga
     2041 aggtcgactt ccttgacatc tcccccaagc agatcgtctc cgcctcgact gcccttatcc
     2101 ccttccttga gcacgacgac gccaacaggg cgctgatggg ctccaacatg cagaggcagg
     2161 ccgttccgct gatcaacgga aaggccccgg tcataggcac ggggatcgag gccagggtcg
     2221 ccagggattc cggttcctgc gtggtggcgc gcagttccgg caccgtgacg agggtagatt
     2281 ccaccagcat cgagatccag
//
LOCUS       DAOPKQ010000222         2249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_56057_length_2249_cov_3.983136, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000222 DAOPKQ010000000
VERSION     DAOPKQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56057_length_2249_cov_3.983136"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK146_06945"
     CDS             complement(<1..489)
                     /locus_tag="PK146_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92010.1"
                     /translation="MGRKSIATLLLPPLVIFSAITVYHLLWIPSPNPVYATIRASGIL
                     AYAFSFLAIVASAFPREITRLTGDPFIRAHHIFAAGGLVLMAIHGLSVLLFFRTLSVF
                     VPDLGSLRDFLMFGGRVALPLFILTAATARFSRSVPFWRKIHILNYAAFFLVTAHALM
                     IGT"
     gene            579..1526
                     /gene="rarD"
                     /locus_tag="PK146_06950"
     CDS             579..1526
                     /gene="rarD"
                     /locus_tag="PK146_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HPE92011.1"
                     /translation="MEASDLSREETGSGLKAAIAAYTAWGLLPVYWKLMVNVPSLGIL
                     SHRIIWSLVFVAMIMIFQGRRRDMAVAFRSPRRAALLSLSSLFIAANWLIYIWAVNAD
                     KVLETSLGYYINPLVTVFFGFVFFREKMERVQWLAISIASVGVLFQVIRYGRFPWVAL
                     GLALSFAFYGLIRKKVVIDSLPGLFAETAFLSIPALLFLLVAGVRYRGAFLAGNPVQD
                     ILLVSTGAVTSLPLLWFAYGARRLKLTTLGFLQYIAPTLTFFLGVSVFREPLDMPKVV
                     TFVCIWVALGLYSWGSFAASRREQGALATVSDPGTGIKR"
     gene            1568..>2249
                     /locus_tag="PK146_06955"
     CDS             1568..>2249
                     /locus_tag="PK146_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPE92012.1"
                     /translation="MDPAKRVRKVPEKILLGPGPTPVEGRVLRAMSEPTLGHLDRDFV
                     AIMDETRDLLRMVFQTGNDLTVAMSGTGSAGMETACVNMIERGDKVIVCSHGVFGSRM
                     QDVAGRCGAGVVEVNAPMGRPVDPGEVEKAIRANPDAKLLGIVQAETSTGVLQPLDEI
                     ASLVHEAGMFMLVDAVTSLGGMEVPVERLGLDMVYSGSQKCLACPPGLAPVTVGPKAV
                     EHIKGRKTK"
BASE COUNT          472 a          590 c          704 g          483 t
ORIGIN      
        1 ggttcctatc atcagcgcgt gggcggtcac aagaaagaat gcggcgtagt tgaggatgtg
       61 tatcttcctc cagaagggga cggagcggct gaaccttgcc gtggcagccg ttaatatgaa
      121 aagggggagt gccaccctcc ctccgaacat gagaaaatcg cgaagacttc caaggtccgg
      181 cacgaacacc gagagggttc tgaagaataa aaggaccgag agtccgtgga tggccatcaa
      241 gacaagccct cctgcagcga agatgtggtg tgccctgatg aaggggtctc cggtaagccg
      301 tgtgatctcc ctggggaacg cagaagctac tatcgccagg aaggaaaaag cgtaggccaa
      361 tatgcccgag gccctgatgg tggcgtagac ggggttggga gagggtatcc acaacaggtg
      421 gtaaacggtg atcgccgaaa agatgaccaa cggtggcaga agaagcgtag caatgctttt
      481 acgtcccatc ccttgagcct cctttttttc tcctggcggt cgccatcttg aagtctaata
      541 gtaacagttt caaagaggat tcgggaggat gaaacagaat ggaagcatcg gatctgtcca
      601 gggaagagac cggatcgggg ttgaaggcgg ccatcgccgc atatacggcc tggggactgc
      661 tcccggtcta ctggaaactg atggtgaacg tcccctccct gggcattctt tcccacagga
      721 taatctggtc gctggttttc gtggcgatga taatgatctt ccaggggcgc cgaagggaca
      781 tggccgtcgc ctttagaagc ccccgcaggg ctgccttgct ttcattaagc agccttttca
      841 tagcggccaa ctggctgatc tacatctggg ccgtcaacgc cgataaagtg ctcgagacta
      901 gccttgggta ctacatcaac cctctggtaa cggttttctt cggcttcgtc ttttttcggg
      961 aaaagatgga gagggtccag tggctggcca tatcgatcgc ctctgtcggg gtgctcttcc
     1021 aggtcatccg ttacggaaga tttccctggg tggccctggg actggcgctt agttttgcct
     1081 tttacggcct gatcaggaag aaggtagtga tcgactccct gccgggtctt ttcgccgaaa
     1141 cggccttcct ctctatcccg gctctcctct tccttcttgt cgccggggtc cggtatcgtg
     1201 gggctttcct tgcggggaac cccgtgcagg acattctcct ggtatctaca ggggccgtta
     1261 cttcgcttcc cctgctatgg ttcgcctacg gtgcccggag gctgaagctg accaccctgg
     1321 gcttcctgca gtacatcgcc cccaccctta cctttttcct cggggtgtct gtattccgcg
     1381 aaccactgga tatgccgaag gtggtaacat ttgtctgcat ctgggtcgct ctggggctct
     1441 actcctgggg gagtttcgcc gcttcccgga gagagcaggg tgctttggcg acggtatcag
     1501 acccaggtac cgggataaaa agataggaca aggggcatta ttttttccac gacagggagg
     1561 gcttgaaatg gatccagcga aacgcgtgag aaaggtgcct gaaaagatcc tcctgggtcc
     1621 cggacccaca ccggtagagg ggagggtcct cagggcaatg tctgaaccga ccctcggcca
     1681 tctggacagg gatttcgtcg ccataatgga cgagacccgg gatctgctga ggatggtatt
     1741 ccagaccggt aacgatctga cggtggccat gtccggcacg gggagcgccg ggatggagac
     1801 ggcctgtgtg aacatgatcg agaggggcga caaggtcatt gtctgctccc acggggtatt
     1861 cgggtcaagg atgcaggacg ttgcgggaag gtgcggcgcc ggggtggtgg aggtcaacgc
     1921 cccgatgggc aggcccgtgg atcccgggga agtcgaaaag gccatcaggg ccaatcctga
     1981 cgcaaaactc ctggggatag tccaagccga gacctccacc ggggttcttc agccgctgga
     2041 tgagatcgca tccctggtac atgaggcagg catgtttatg ctagtcgatg ccgtgacgtc
     2101 cctgggagga atggaggtac ccgtggaacg cctcggtctg gacatggtct acagcggaag
     2161 ccagaagtgc cttgcctgcc ccccgggact tgcacctgta acagtagggc cgaaagccgt
     2221 ggagcatatc aagggtcgta agaccaagg
//
LOCUS       DAOPKQ010000223         2249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_56059_length_2249_cov_3.940292, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000223 DAOPKQ010000000
VERSION     DAOPKQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56059_length_2249_cov_3.940292"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /gene="mrdA"
                     /locus_tag="PK146_06960"
     CDS             complement(<1..1467)
                     /gene="mrdA"
                     /locus_tag="PK146_06960"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPE92013.1"
                     /translation="MSSDRISLPTRLNVWRAIIMVSLVVLVVALFRLQVLQSDLYVNL
                     AARNRLRLVRMPPARGRILDVNGTVLATNVQTFDLMVYPLDLQDKETAEEVSRFLKAR
                     GIPLEVEQMLERVRKEFTVPYRAVTLLPDLTLAQLSSLVSDPDFPRQLFPVPVWRRVY
                     PAGPLVAHVLGYVGEVTREELERFSPGDYRGGDHVGKGGVEEFYESVLRGTPGQEAIE
                     VDARGRKVKRVGFIEPGQGEDLELALDLGAQRLASELMAGRVGAVFAMDVHNGNVKVL
                     FSSPNFDANPLAWGISSREWNELMRDPVRPMLNRVIAGTYPPASTFKVVPAFAALAEN
                     AVTRRTTFTCTGGMRVGNRFFRCWKRDGGHGPVNVVKGLRDSCDVFFYQAGLRTGIDA
                     LVKWGAVFGVGEPTGIDLPGEARGNIAGPAWKLERFRERWYPGDTANYSIGQGFLLLT
                     PIQLARIYAAIANGGKLVTPRLLKGAKTSVSLQLPEGPL"
     gene            complement(1454..1921)
                     /locus_tag="PK146_06965"
     CDS             complement(1454..1921)
                     /locus_tag="PK146_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92014.1"
                     /translation="MVPVIFSWYVQDLFQVLLSGKFLVPDIFLVFLIYRMTREPKDVL
                     SVVWPAFVGGFLWDLRWTALPGLTAAIYSLIAGVCVVVWNQVPDSGRNARLFLVLALS
                     AQILAGLVRFISWGSSRGALVEALAFQQFSALPVVIVAALVVAAGVDKDNVKR"
     gene            complement(1923..>2249)
                     /locus_tag="PK146_06970"
     CDS             complement(1923..>2249)
                     /locus_tag="PK146_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPE92015.1"
                     /translation="DHSWVDILTSTHLYVPVVIDETRDLGVLAGNGKGEIDLLYIPEY
                     KVLRPGMRISTALVSDILPAGIPVGEISEKEPDIEGGFRVYHVSPGADISVMDSLLIM
                     TGEGRS"
BASE COUNT          497 a          708 c          624 g          420 t
ORIGIN      
        1 aaggggtccc tctggcagct gcagagatac cgaggtcttg gcccccttta gcagtctcgg
       61 cgtcacgagc ttgccgccgt tcgcgatggc agcgtagatc ctcgccagct ggatcggagt
      121 aaggaggaga aaaccctgtc ctatggagta gttggctgta tccccggggt accatctctc
      181 cctgaaacgc tccagtttcc aggcgggccc ggcaatgttg ccccttgcct cgcctggcag
      241 gtctatgccc gtaggctcgc ctaccccgaa taccgcaccc catttgacca gggcgtctat
      301 tcccgtcctc aggccggcct ggtagaagaa aacgtcgcaa gaatcacgga gccccttcac
      361 aacgttaacg ggaccgtgcc ccccatccct cttccagcac cggaagaatc tgttccccac
      421 cctcatgccg cccgtgcagg tgaaggtcgt tcttctggtc actgcattct ccgccagggc
      481 agcgaaggcg ggcaccacct tgaaagtcga agctgggggg tacgtgccgg cgatcaccct
      541 gttaagcatc ggccttacgg gatccctcat cagttcattc cactctctcg aggagatccc
      601 ccaggcaagc gggttggcgt cgaagttcgg ggacgagaag aggaccttca cgttcccgtt
      661 atggacgtcc atggcgaaga ccgcccccac cctgcctgcc ataagctccg aggcaagcct
      721 ctgggctccg agatcgaggg caagttccag gtcctctccc tgtccgggtt ctatgaagcc
      781 cacccgtttg accttacgcc ccctggcatc cacctctatt gcctcctggc cgggggtacc
      841 ccttagaaca ctctcgtaga actcctcgac cccgcccttg cctacatggt cccctccacg
      901 gtagtcaccg ggagaaaacc tctcaagttc ctccctggtc acctctccga cgtacccaag
      961 gacatgggcc accaggggac ccgccgggta gaccctcctc cagacaggaa cggggaagag
     1021 ctgtctcgga aagtccgggt cgctcaccag cgatgaaagc tgggcaagag tgagatcggg
     1081 aagaagggta accgccctgt aaggtacggt gaactccttc ctgaccctct cgagcatctg
     1141 ttccacttcc agggggatac ccctggcctt gaggaaacgg ctgacctcct cggcagtttc
     1201 cttgtcctgc aggtccaggg ggtagaccat caggtcgaag gtctggacat tggtggcaag
     1261 aacagtcccg ttgacgtcaa gtatcctccc cctggcggga ggcatcctga cgagccgaag
     1321 cctgttcctg gcggcaaggt tgacgtagag gtcggactgg aggacctgga gcctgaaaag
     1381 ggccacaacc aggaccacca gggagaccat tataatggcc ctccatacgt tgagcctggt
     1441 agggagggag atcctatcgc ttgacattat ccttgtccac tccagctgcc accacgaggg
     1501 ccgccacaat taccaccgga agggccgaga attgctggaa ggccaaagcc tcgacgaggg
     1561 ctcccctgga ggacccccag gagatgaacc ggacgagacc ggcaaggatc tgtgccgaaa
     1621 gcgccaggac caggaaaagc ctggcattcc tccccgagtc gggcacctgg ttccagacca
     1681 ccacgcagac tcccgcgata agcgaataga tagcggcggt aagtccggga agagcagtcc
     1741 atctcaggtc ccacaggaaa ccgcccacaa aagccggcca gaccaccgaa agaacatctt
     1801 tgggctctct ggtcatcctg tagatcaaaa agaccaggaa gatatcgggg acaaggaact
     1861 tccccgaaag aagcacctgg aaaaggtcct gaacgtacca ggaaaagata acggggacca
     1921 tgtcaggacc ttccctcccc ggtcatgatg aggagggagt ccataaccga gatatcggcg
     1981 cctggagaga catggtatac cctgaatcca ccctcgatgt cgggttcctt ttcggatatc
     2041 tctcccacag gtatccctgc gggaagtata tcgctgacca aagcggtgct tatgcgcata
     2101 cccggacgga ggaccttgta ctcgggaatg taaaggaggt ctatctctcc ttttccgtta
     2161 ccggccagga cccccaggtc cctggtctca tctatgacca ccggtacata aagatgcgta
     2221 gaggtcagaa tatccaccca ggagtggtc
//
LOCUS       DAOPKQ010000224         2249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_56062_length_2249_cov_3.500000, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000224 DAOPKQ010000000
VERSION     DAOPKQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56062_length_2249_cov_3.500000"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PK146_06975"
     CDS             complement(<1..746)
                     /locus_tag="PK146_06975"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HPE92016.1"
                     /translation="MAKKTGTAPSRRTTKSRTRSKPVKKASQSGKKAGRTLVIVESPS
                     KARTLSKILGREYDIQSSVGHIRDLPKSRLAIDIENGFEPEYILVRGKGDIARNLRER
                     ASVSGRVLIASDPDREGEAIAWHLSGILGIDPTSLCRVRMYEITQKGVREAFEKVSTV
                     DMKKVDAQQARRILDRLVGYKLSPLLWNKIKRGLSAGRVQSAALKIICDREREIESFV
                     PQEYWHVPVAAEDEGSRNYSLRVDRLDGKSL"
     gene            complement(761..1003)
                     /locus_tag="PK146_06980"
     CDS             complement(761..1003)
                     /locus_tag="PK146_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92017.1"
                     /translation="MSVRFIPEGESRFLTDDALALVYRVCADETLSREVIENALTEAV
                     RLSLIGDCPVNADLFEALLQSICERQTAGLKELPLC"
     gene            complement(1035..2120)
                     /gene="dprA"
                     /locus_tag="PK146_06985"
     CDS             complement(1035..2120)
                     /gene="dprA"
                     /locus_tag="PK146_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPE92018.1"
                     /translation="MLNACGCFDARILTIIESRRISWEDLASGSGAAMDRLGMKESAR
                     KEFLGLLSSGWYDREVESCESMGVRLIFSGDPGYPSMLADMIDPPLVLYESGSGLPSG
                     ERVAVVGTRRCTSYGSNCASDLASVLVDVGVAVVSGGARGIDAAAHRGCMSSGGRTVA
                     VTGTGADISYPASHSALFREIREEGCLLTEFPLGTPPRPWNFPRRNRLIAGLCSRCVV
                     VEAPEKSGAVLTARLALEAGREVWAVPGRITDGVCKGSNALISDGAMPLVDIRVFAAL
                     VSGRQGALFPTGGRREDTAGPVLTPEEAEVLRILADHGEMTVDNISAEGKMSAAVVLR
                     GLGVLSAYGLVAPGGPGRWSKRTQGRG"
     gene            complement(2135..2242)
                     /locus_tag="PK146_06990"
     CDS             complement(2135..2242)
                     /locus_tag="PK146_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92019.1"
                     /translation="MARTIADLAGEENLSTVHLAEALAFREGGDLPWDR"
BASE COUNT          410 a          755 c          601 g          483 t
ORIGIN      
        1 agcgacttgc cgtcgagcct gtcgaccctc agcgagtagt tcctgctccc ctcgtcttcg
       61 gcggccaccg ggacgtgcca gtactcctgg ggcacgaagg attcgatctc cctctccctg
      121 tcgcagatta tcttcagggc tgccgactgt accctcccgg cggagagccc cctcttgatc
      181 ttgttccaca gcagcgggct cagtttgtag ccaacgagcc tgtccaggat ccgcctcgcc
      241 tgctgggcgt cgaccttttt catgtccacg gtggagacct tttcaaaggc ctcccttacc
      301 cccttctggg tgatctcgta catcctcacc cggcagaggg aggtggggtc gatcccgagg
      361 ataccagaga ggtgccaggc aatggcttct ccctccctgt cagggtcgga agcgattagc
      421 acccttccgc tcaccgaggc cctttccctg aggtttcggg ctatatcgcc ctttcccctt
      481 acaagaatgt attctggttc gaagccgttc tcgatatcga tggccagcct gctctttgga
      541 aggtcgcgaa tatgccccac actggactgg atatcgtatt ccctgcccag gatcttgctc
      601 agcgtcctgg ccttggatgg cgattcaacg ataaccaggg tcctgcctgc ttttttcccg
      661 gactgggaag cctttttcac aggcttcgac ctggttctgg actttgtggt ccttctggag
      721 ggagcggtcc cggtcttctt tgccatcaga tctccaccct tcagcaaaga ggaagttcct
      781 tcagtcctgc cgtctgtctt tcgcagatgg actgcaggag ggcctcaaaa aggtcagcgt
      841 tgacaggaca gtcgcctata aggctcaggc ggaccgcctc tgtcagggcg ttctctatta
      901 cctctctcga gagggtttca tctgcacaga cccggtagac aagagccagg gcatcatcgg
      961 tgaggaatcg gctctcgccc tcggggatga atcttacgga cattatctct cctcctgttc
     1021 ccatctgtgg cccgtcagcc tcttccctgg gtcctcttgc tccacctgcc gggaccacca
     1081 ggggcgacga ggccgtatgc cgagagtacc ccaaggcccc ttagcacgac agcggcgctc
     1141 attttacctt ccgccgagat gttgtcaacc gtcatttcac cgtgatcggc caggatccga
     1201 agaacctctg cctcctccgg agtgaggaca ggacctgccg tatcctcccg acgcccacct
     1261 gtggggaaga gcgccccttg tcttcccgag accagtgccg cgaagacccg gatatccacc
     1321 agaggcatag ccccgtccga tatgagggcg ttcgaccctt tgcagacgcc gtcggtgata
     1381 cgaccaggca cagcccatac ctccctgcct gcttcgagtg caagcctggc ggtcaggacc
     1441 gcgccgctct tctcaggggc ttcgacgacc acgcacctgg agcacaggcc ggctatcagc
     1501 ctgttgcgcc tggggaagtt ccatggcctt ggaggcgtcc cgaggggaaa ctccgtcaga
     1561 agacagccct cttctcttat ctccctgaac agagccgagt gggaggcggg ataggagata
     1621 tccgctcctg ttcctgtcac agcgacggtc ctgccgccgg aagacatgca ccccctgtgc
     1681 gcggcggcat cgatccccct ggcccccccg cttactactg caacgccgac atcgaccagg
     1741 acggaagcca ggtccgatgc gcagttcgag ccgtaggagg tacacctcct ggtacctacg
     1801 accgccactc tttccccact gggtagcccc gaaccgcttt cgtatagcac cagaggcgga
     1861 tcgatcatgt cagccagcat ggacgggtag ccgggatcac cggaaaagat cagcctgaca
     1921 cccatacttt cgcaactctc gacctccctg tcgtaccacc ccgacgatag cagacccaga
     1981 aactcctttc gggcagattc cttcatccca agtctgtcca tggccgcgcc agacccggag
     2041 gcaagatcct cccaggatat cctccggctc tcgatgatcg tcaagatcct tgcatcgaaa
     2101 cacccgcagg cgttcagcat gagcatcgcc ctctttaccg gtcccagggc aggtcacctc
     2161 cttccctgaa ggccagggcc tcggccaggt ggaccgtcga aagattttct tcgccagcca
     2221 ggtcggctat agtcctggcc accttgagg
//
LOCUS       DAOPKQ010000225         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_56256_length_2243_cov_2.947441, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000225 DAOPKQ010000000
VERSION     DAOPKQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56256_length_2243_cov_2.947441"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PK146_06995"
     CDS             <1..596
                     /locus_tag="PK146_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPE92020.1"
                     /translation="YHRMEYYHDHGHIHDYSIPRGAEGKACLGFNFRMSEIQGALGLV
                     QLDKLDGAIASLRSNKKRVLEGIELPADVRLRDLPDREGEIATQLVLLLPDAESAKRF
                     QKAAGDAGCGCGILSGNTWHYARHWETLREGAYYSRIRCPYDCPYAEDVPLYRPVEWP
                     RTHDILSRAAVYVMDIFMDDIRIASMQKAIRAGLKAL"
     gene            607..1710
                     /locus_tag="PK146_07000"
     CDS             607..1710
                     /locus_tag="PK146_07000"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HPE92021.1"
                     /translation="MSEGAGPGIVFVVSDGIRGHENQSVGIAWWLSEICGARLKTLEI
                     PKISGWSRFLWYKIRSRTLRKGTDGACRAWLGSVPEGESLLREVKKILEDSSVEGDRP
                     FFISAGSSAAPWCLALARVLGGRCCTIMTPSVLGTKPFDFAIVPEHDFPISAPNTLVT
                     LGAPNMIRPDLLEVESARLARMFPAAQEVRWAILIGGDDANYALSPSWVRKNIPLLLD
                     QAEMSGADVYITTSRRTSPAAEEAIIFAVRGRKSVRALILASREDWNPVPGMLGLCGK
                     IFCTEDSVSMISEAFTAGCEVEILRVERRRGIRALLQEAIRWLVSRGLISRKFLWGVP
                     RFDMMIDSFGRIDENDFNEARRAAMWMIEEWGV"
     gene            1683..>2243
                     /locus_tag="PK146_07005"
     CDS             1683..>2243
                     /locus_tag="PK146_07005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPE92022.1"
                     /translation="MDDRGVGRLKIAQILPEFEEGGVERHVLWLSKALSEMGHDVTVI
                     TAGGKLEEQLDPKVALVRLPVHKKNPVTALYCAISIASRTKREGWDILHAHSRVPAWI
                     AWWVAGFSGRPWVATAHAQYGLNAGLIPYRKANGVICVSRTVREHLGSFLPEKSEVIP
                     NGIPPARRKWEGKGFPENPRFLFVGRL"
BASE COUNT          496 a          592 c          677 g          478 t
ORIGIN      
        1 tctaccacag gatggagtat tatcacgatc atggccatat ccacgactat tccatccctc
       61 gtggagccga agggaaggcc tgccttggct tcaatttccg gatgagcgag atccaggggg
      121 ctctcggact tgttcaactt gacaaactgg atggggccat agccagcctc aggagcaaca
      181 agaagagggt tcttgaagga atagaacttc ccgcagatgt gcggctcagg gatcttcccg
      241 accgggaggg ggaaatagcg acacaacttg tgctgcttct gcccgacgcg gaatcggcga
      301 aacgcttcca gaaggccgcc ggtgacgcgg gctgcggatg cggaatactt tcgggcaaca
      361 cctggcatta cgccaggcac tgggagaccc tcagggaagg tgcatactac agccggatca
      421 gatgccccta cgactgcccc tacgcagagg acgttccact gtacaggccc gtggagtggc
      481 ccaggaccca tgacatcctt tcccgcgccg ccgtctacgt gatggacata ttcatggatg
      541 acatcaggat cgcgtccatg cagaaagcga taagggcggg gctgaaggcc ctttaggtta
      601 ttttaaatga gtgagggagc ggggccgggc attgtcttcg tcgtctcaga cggtattcgg
      661 gggcacgaga accagtccgt gggcatagcc tggtggctgt cggagatttg tggcgcgcga
      721 ctgaagaccc ttgaaattcc gaagatcagt ggctggtccc gtttcctctg gtacaagatc
      781 cggtcccgga ccctcaggaa agggacggat ggggcctgtc gagcctggct cgggagtgtt
      841 ccagaagggg aaagtctcct tcgggaagtg aaaaaaatcc ttgaagattc ttccgttgag
      901 ggagaccggc cattctttat ttcagccggg agctccgccg cgccgtggtg cctggccctt
      961 gccagggtcc tcggtggcag gtgctgcacg ataatgaccc cttcggtcct cgggacgaaa
     1021 ccctttgatt ttgccatcgt gccggagcac gattttccca tatcggcacc aaacaccctc
     1081 gtgaccctcg gtgcccccaa catgatccgt cccgatcttc tcgaggtaga atcggccaga
     1141 ctggcccgga tgtttccggc agcccaagaa gtcagatggg ccatactgat cggtggagat
     1201 gacgcgaact atgccctttc cccatcgtgg gttcgaaaga acattccgct tcttctcgac
     1261 caggccgaaa tgtccggtgc cgacgtttac atcactacgt cccgaaggac aagtcccgcc
     1321 gcggaagagg caatcatctt cgctgtacgg gggagaaaaa gcgtgagggc cctgatcctg
     1381 gcatcccggg aggactggaa ccctgttccc ggcatgcttg gcctctgcgg gaagatcttc
     1441 tgcacagagg actccgtttc gatgatatca gaggcattca cggcagggtg cgaggtcgag
     1501 atactcaggg tcgaaaggcg aagaggcatt cgcgcccttc ttcaggaggc gatccgatgg
     1561 cttgtatcga gaggtctcat ttcccgaaag ttcctctggg gtgtaccacg tttcgatatg
     1621 atgatagact cctttgggcg aatagatgaa aatgacttta acgaggcccg cagggcagcg
     1681 atgtggatga tagaggagtg gggcgtctga agatcgcgca gatactgccg gagttcgaag
     1741 aagggggtgt cgagcgtcac gtattgtggc tttcgaaagc tctctcagaa atgggtcacg
     1801 atgtcaccgt cattaccgcg ggaggcaaac tggaagaaca actcgatccg aaggttgctc
     1861 tcgtcaggct gccagttcac aaaaaaaacc cggttactgc actttattgc gctatttcaa
     1921 ttgccagcag gacaaaacgc gaagggtggg atatccttca cgcccactcc agggttccgg
     1981 cctggatagc ctggtgggtg gccggtttct cgggaaggcc gtgggtggcg acggcccacg
     2041 cccagtacgg tctcaacgcg ggtcttatac cttacaggaa ggccaatggt gttatctgtg
     2101 tcagcaggac cgtacgggaa catcttggat cttttcttcc ggaaaaatca gaagttattc
     2161 caaacggcat tcccccagcc agaagaaagt gggaggggaa aggttttccc gaaaatccca
     2221 ggtttctttt cgtggggagg ttg
//
LOCUS       DAOPKQ010000226         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_56259_length_2243_cov_2.820384, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000226 DAOPKQ010000000
VERSION     DAOPKQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56259_length_2243_cov_2.820384"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PK146_07010"
     CDS             <1..412
                     /locus_tag="PK146_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HPE92023.1"
                     /translation="REYGNTGIYRDRGEDRDIMVVDRVGVLFDLYGSADAAFVGGSLV
                     PRGGQNILEPAVWGVPFAHGSHMEDFAPHAKALGSLGASRMVRGSSELAREWERMISG
                     GGNPGLAGRKYVENLGGASALAWNRISDILKGRE"
     gene            431..1579
                     /locus_tag="PK146_07015"
     CDS             431..1579
                     /locus_tag="PK146_07015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPE92024.1"
                     /translation="MFMAGRIQFGPGVSSKAGDLVRGTGAKSALIVTDETMERLGVAE
                     RITEAVRKGGVEVSVFSKVEPEPSVETTDEAAKIARGMNAGAVIGLGGGSSMDVAKAV
                     AVLVNNEGSAANYQGLGLVKKPGVPKIMIPTTSGTGSEVTFTAVLIRKSDGVKGGINS
                     DFLYPEMSLLDPELAVTMPPRVTASTGMDALAHALEAYTSLKASPFSDIFAEEALVRI
                     GEWLRVATFNGNNIEARGEMMLAALFGGVALANAGVTACHALAYPLGGMFGVPHGIAN
                     ALLIPHVARWNAMASPSRFTAAAELLGYPREEGEPEHVSAMLCSEALGELADDLGLPE
                     TLEDLDVGITEDHFEEMAIKAMGVARPMENNPRVMTKDDCVKIYMEAM"
     gene            1582..>2243
                     /locus_tag="PK146_07020"
     CDS             1582..>2243
                     /locus_tag="PK146_07020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPE92025.1"
                     /translation="MPGFELFDQREIDAVTDVIKRKMVHRYSFQETRNDIYRVEEFEK
                     AVAAKFGAKHCLAVSSGSAALYVALRGLGIGPGDEIITSPFTFIASVEAILECGAVPV
                     LAEIDESLNLDPESVEELITERTRAIMPVHMFGAAADLDAFVEICEEYGLWLLEDSCQ
                     AMGATYDGRFVGTFGKWGPYSLDPYKLLTVGEGGLIVTDDEEVYHRMEYYHDHGHIHD
                     YS"
BASE COUNT          498 a          586 c          729 g          430 t
ORIGIN      
        1 ccgggaatac ggaaacacag gcatctacag ggacaggggt gaggacaggg atatcatggt
       61 cgtggacagg gtaggggtgc tgttcgacct ttacggttcg gccgacgccg ccttcgtggg
      121 aggtagcctt gttcccaggg gggggcagaa tatacttgag ccggccgtat ggggtgttcc
      181 ctttgcccat ggttctcaca tggaggattt tgctcctcac gccaaagccc tcgggtcact
      241 gggggcatcc aggatggtca gagggtcatc ggaactggcc cgggagtggg aaagaatgat
      301 atcaggcgga ggaaaccccg gactggcggg gcggaaatac gtggaaaatc tcggcggggc
      361 atctgccctg gcgtggaatc ggataagcga tattttgaag ggcagggagt gatagaaggt
      421 ggatttcgga atgtttatgg cggggcggat acagttcggc cccggggtct catcaaaagc
      481 gggagacctg gtccggggaa ccggcgcaaa gagcgcgctg atagtgaccg acgagacgat
      541 ggaacggctc ggagtggccg aaaggataac ggaagcagta cgtaaaggcg gtgtggaagt
      601 atcggtcttt tcaaaggtcg agccggaacc ttctgttgag accaccgacg aggccgcaaa
      661 gatcgccagg ggcatgaatg ccggggccgt gatcggtctt ggcggaggga gttccatgga
      721 cgtggccaag gcggtcgccg ttcttgtcaa caacgaaggt tccgccgcga attaccaggg
      781 acttggcctg gtgaagaagc cgggtgtccc taaaataatg atacccacaa catcgggaac
      841 gggttcggag gtcaccttca cggccgtgct cataagaaag agcgacggag tcaagggtgg
      901 tataaacagc gattttctct accccgagat gagccttctg gacccggaac ttgccgtgac
      961 tatgcctccc cgggtgacag catcgactgg catggacgcc ctcgctcacg cccttgaggc
     1021 atatacgagc ctcaaggcct ctcccttcag cgacatcttc gcggaagagg ctctggtcag
     1081 gataggggaa tggctgcggg tcgccacctt caacggcaac aacatagagg ctcgcggaga
     1141 gatgatgctt gccgcccttt tcggcggtgt tgccctggcc aacgcgggag ttacagcctg
     1201 ccatgccctg gcctaccccc ttggaggaat gttcggcgtt ccccacggga tagccaacgc
     1261 acttctgata cctcacgttg cccgctggaa cgccatggca tcgccctcca ggtttaccgc
     1321 cgcggcggaa ctgctggggt acccacggga agaaggggag ccggagcacg tctctgccat
     1381 gttgtgttcc gaggctcttg gagaacttgc cgatgatctt ggccttcccg agacgctgga
     1441 agaccttgac gtggggatca ccgaggatca tttcgaggag atggcaataa aggcgatggg
     1501 agttgccagg cctatggaga ataacccgcg cgtcatgaca aaggatgact gcgtgaaaat
     1561 ttacatggag gcgatgtagc gatgcccgga tttgaactgt tcgaccagag ggagatagac
     1621 gccgtaacag atgttatcaa aagaaagatg gtccacaggt actcattcca ggagacgagg
     1681 aacgacatct acagggtgga ggagttcgag aaggccgttg cggcaaagtt cggcgcgaaa
     1741 cactgcctcg ccgtcagcag cggcagcgcg gcgctttacg tggccttgag aggcctcggt
     1801 atcggtcctg gagacgagat cataacgtcc cccttcacct tcatcgccag cgttgaggcc
     1861 atcctcgagt gcggcgccgt cccggtcctt gcagagatcg acgagagcct caacctggac
     1921 cccgaatcag tggaggaact catcacggaa cgcaccaggg ccataatgcc ggtgcacatg
     1981 ttcggtgccg cggcagacct tgacgccttt gtagagatat gcgaggagta cgggctctgg
     2041 ctgctggagg attcctgcca ggccatgggg gcaacctacg acggccgatt cgtcgggacc
     2101 ttcggcaagt gggggcccta cagcctggat ccctacaaac ttcttacagt gggtgagggc
     2161 ggtctcatcg ttaccgacga cgaggaagtc taccacagga tggagtatta tcacgatcat
     2221 ggccatatcc acgactattc cat
//
LOCUS       DAOPKQ010000227         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_56310_length_2241_cov_4.060842, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000227 DAOPKQ010000000
VERSION     DAOPKQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56310_length_2241_cov_4.060842"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PK146_07025"
     CDS             complement(<1..404)
                     /locus_tag="PK146_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPE92026.1"
                     /translation="MKKILGILGSPRKGGNSETLTERVLVCAGERGAMTDVIRLQGLK
                     LSGCIDCRKCWSRGSPCVIDDDMGKVHDRIREADVLVFASPLYWYTWSSQMKPVWDRF
                     LPFLHDEAGWDLKGKKAVLVATAGDDKPDAFNG"
     gene            complement(401..1366)
                     /locus_tag="PK146_07030"
     CDS             complement(401..1366)
                     /locus_tag="PK146_07030"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPE92027.1"
                     /translation="MRFYYNGVLPIAKNLLKGSAVLELEGIYAPIPTPFNDDGNLDLD
                     ALSLNFDKWLRTPLDGIVASGSNGEWPLLSFPERAALFKACADLSGGRLKVIAGIHCP
                     STKESLDLGIEAARAGCDAVLVLPPHYYKGQNSREGLLAFFRTIADSVRLPVVLYNMP
                     SNTGFNLAPDIVATLSEHPNIIGIKDSSGDIVQIASICRDTPEDFGVFAGSGSFFLPS
                     LAVGCSGGTMGVANLFADACRDILLSFRKGDLERSRSLQLSIIEINQAVTKWFGVPGL
                     KAAMDHAGLYGGPVRPPLLPVEGKVRQEIGRIYDSFRSNYREVLS"
     gene            1401..>2241
                     /locus_tag="PK146_07035"
     CDS             1401..>2241
                     /locus_tag="PK146_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPE92028.1"
                     /translation="MDDLRVGTCSWSDRSLLASGWYPEKAKDPNSRLQFYSNSFDTVE
                     VDSTFYAIPGEEVFYQWVSRTAPGFLFNVKAFGLFTFHSLSLSALPGKLAPSGLSRGS
                     RLRIWEIPKSVRIALWDMFISRVLILNSMNRLGYLLFQFPPWVVYSEKNLNWFKRIGK
                     LAAPARVAIEVRHNSWLEEGNRERFMDLLRDQNMAFTAVDEPRLEWTVPPEWHITATW
                     GTVMRFHGRNTVAWNKREAKVAERFNYLYDISELGPFSERAQALAKEVGRVFLMFNNC
                     FRDN"
BASE COUNT          495 a          615 c          634 g          497 t
ORIGIN      
        1 ccgttgaagg catctggttt gtcgtccccg gctgtcgcca ccaggacagc cttctttccc
       61 ttgaggtccc aaccggcctc atcgtggagg aaggggagga agcggtccca gacaggtttc
      121 atttgtgaac tccacgtgta ccagtacaga ggggaggcga agacaagaac gtccgcttcc
      181 cttatcctgt cgtggacctt tcccatgtcg tcgtcaatca cgcaggggga gcccctggac
      241 cagcacttcc tgcagtcgat gcatccggaa agtttcagcc cctgaaggcg tattacatcg
      301 gtcatggctc ccctttcccc ggcgcagacg aggacccttt cggtcaaggt ctcgctgttg
      361 cctcccttcc ggggacttcc caggatccca aggatcttct tcacgataac acctccctgt
      421 aatttgatct gaaagagtcg tagatccgtc ctatctcctg ccttaccttt ccttccaccg
      481 gaaggagggg aggcctgacg gggcccccgt aaaggcccgc atggtccatg gcggccttga
      541 gaccgggcac accgaaccat ttggtgacag cctggtttat ctcgataatg gagagctgca
      601 gggaccttga acgttccaga tccccctttc ggaaagagag aaggatgtcc ctgcaggcgt
      661 cggcgaagag gttggcaact cccatggtac ctcctgaaca ccccacggca agtgagggga
      721 ggaagaaact ccccgatccg gcaaagaccc cgaagtcttc aggggtatcc cggcatatgg
      781 atgcgatctg tacaatatcg ccggagctgt cctttattcc gatgatattc gggtgctctg
      841 aaagagtggc cacaatatca ggggcaaggt tgaaacccgt gttagagggc atattgtaaa
      901 gaaccaccgg gagacgtacc gaatcagcga tggtccggaa gaatgcaaga aggccttccc
      961 tggagttctg ccccttgtag tagtggggag gcagcacaag gacagcatca cagcctgccc
     1021 ttgcggcttc gatcccgagg tcgagactct ccttcgtgga cgggcagtga atccccgcta
     1081 ttaccttgag cctccccccg gaaaggtccg cacaggcttt gaagagagct gccctttcag
     1141 ggaaactgag gaggggccac tccccgttgg aaccactcgc gacgattccg tcaagtggtg
     1201 ttctcaacca cttgtcgaag ttcagggaga gcgcgtcgag gtcaaggttc ccgtcgtcgt
     1261 taaagggtgt gggtatcggt gcgtagatcc cttccagttc caagacggca cttcctttca
     1321 gaaggttttt tgctatcggc aggactccat tataatagaa tcgcaagcgg atgccccatg
     1381 ttctttcgga gggcaaggca atggatgacc ttagggtagg gacctgttca tggtctgacc
     1441 ggtcgcttct ggcgagcggt tggtacccgg agaaagcaaa agaccccaat tcaaggcttc
     1501 agttttattc gaactccttc gataccgtcg aggtggacag taccttctac gccattccgg
     1561 gcgaggaggt gttctatcag tgggtttcga ggaccgctcc cgggttcctt ttcaacgtca
     1621 aggccttcgg cctcttcacc ttccattcac tgtccctgtc ggctcttcct ggaaagctgg
     1681 caccctcagg tctctcaagg ggttccagac tgaggatctg ggagatcccc aaaagtgttc
     1741 ggatcgccct ctgggatatg ttcatctcga gggtcctgat actcaacagc atgaacaggc
     1801 ttggttacct gctattccag ttccccccgt gggtggttta ttctgaaaag aacctgaact
     1861 ggttcaaaag gatagggaaa ctggcggccc ctgccagggt ggccatagag gtaagacaca
     1921 actcctggct cgaagagggg aacagggaaa gattcatgga ccttctgaga gaccagaaca
     1981 tggcctttac cgctgtggat gaacccaggc ttgagtggac ggtccctccc gaatggcata
     2041 ttaccgcaac atggggcacg gtgatgaggt tccacgggag gaacactgta gcgtggaata
     2101 aaagggaggc caaggtggcg gagcgcttca actatctgta cgatatatcc gaacttggac
     2161 ctttcagcga gagggcccaa gccctggcaa aagaggtcgg cagagtcttc ctgatgttca
     2221 acaactgctt ccgagacaat g
//
LOCUS       DAOPKQ010000228         2240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_56341_length_2240_cov_5.572540, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000228 DAOPKQ010000000
VERSION     DAOPKQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56341_length_2240_cov_5.572540"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK146_07040"
     CDS             complement(<1..192)
                     /locus_tag="PK146_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPE92029.1"
                     /translation="MSVRTIREYPDPVLRGRAEDVTDFGEELQSLLKDMWETMEKNDG
                     VGLAAPQIGVNLRVAVIGWR"
     gene            complement(262..1704)
                     /locus_tag="PK146_07045"
     CDS             complement(262..1704)
                     /locus_tag="PK146_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPE92030.1"
                     /translation="MESFLEKYEVGAIHRGKTVEGTVVESNEDGWLVDVGFKCEGFLP
                     RNEWSHRSLVEAKDVPSVGDTVRVQVVSLKQGEEAQLIVSRWRTEFDDRWDAMESSLT
                     GDDVLEVRGLRKVKGGLIVDCCGIEGFIPVSHLAEEGRGINPSRLVDDTFSAKVIERD
                     RRKRRIVLSRRALLENELMEEKRSFFEKTEAGQTLEGTVTSITSFGAFVNLGPVEGLV
                     HNTELTWGRNVKPKEIVNKGDRVMVKIIGIDPENTRVSLSIKQTQPDPWDSVAERYTE
                     GEVLTGKVTNLADFGAFVELEPGIEGLVHIGDISWQRIRHPREVLKKGQKVEVQILSV
                     DGENKRISLGYKQLHDPWKDIEEKYQVNGVYPVKIVRLTDFGAFVELEDGIEGLIHVS
                     QISRKRVEKPRDVVSEGQEVQARIIEIKPNERRIRLSMSVLEEPEPRKEREPEKRRRK
                     NEPRNDPSKFMDGETNVTIGDIIKESLQEE"
     gene            complement(1773..>2240)
                     /locus_tag="PK146_07050"
     CDS             complement(1773..>2240)
                     /locus_tag="PK146_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPE92031.1"
                     /translation="SSEGPVTVVDPSRLKSFEIEGLKKVGIVSQTTQRKEDLSALVSN
                     VSARASETRVFNTICDATSSRQGSVARLAGSVDGIIIIGGKNSANTEKLFEIANTSGT
                     PTLWIEDEEGLERGWLEDKRRIGIAAGASTPDWLIHRIATRLASAKSGREDGW"
BASE COUNT          384 a          733 c          538 g          585 t
ORIGIN      
        1 tctccacccg ataacggcca ccctcaggtt gacccctatc tgaggcgccg caagacccac
       61 gccatcgttc ttttccatgg tctcccacat gtctttcaga agggactgga gttcttctcc
      121 aaaatcagta acgtcctccg cccttcccct gaggaccgga tcggggtact cccggatagt
      181 tcttactgac atccagcgat cctccccttc tttcttcctt gaaaagcggc cccctgaaga
      241 gggccgcctg ttggtccggt actactcttc ctgcagggac tccttgataa tgtccccgat
      301 ggtcacattg gtctccccgt ccatgaactt ggaggggtcg ttgcggggct cgttcttcct
      361 gcgcctcttt tcgggctccc tctccttccg gggttcgggc tcctcgagta cgctcatgct
      421 tagccggatc cttctctcat tgggcttgat ctctatgatc cgggcctgga cctcctgacc
      481 ctctgagacc acgtccctgg gtttctcgac ccgtttgcgg ctgatctgtg agacgtggat
      541 cagaccctcg atcccatcct caagctccac aaaggctccg aaatcggtca gacgtactat
      601 ctttacagga tagacaccgt tcacctggta cttctcttcg atatccttcc agggatcgtg
      661 gagctgtttg tagccgaggc tgatcctttt gttctctcca tcgacggaaa gtatctggac
      721 ctccaccttt tgccccttct tgaggacctc tctggggtgg cggatcctct gccagctgat
      781 atcgccgata tggacaagcc cttcgatccc gggttcaagc tccacgaagg caccgaaatc
      841 ggccaggttg gtgaccttgc ccgtcagcac ctcgccttcg gtgtagcgtt ctgccacact
      901 gtcccaaggg tcgggctggg tctgttttat gctcagggac acccgggtgt tctcagggtc
      961 gataccgatg atcttgacca ttacccggtc gcccttgttt acgatctctt tcggcttgac
     1021 gttcctcccc caggtgagct ccgtgttgtg cacaagccct tcaacgggcc ccaggttgac
     1081 gaaggccccg aaggaggtta tggacgtgac cgtcccttcc aacgtctgcc ctgcctcggt
     1141 cttttcgaag aaggaccgtt tctcctccat gagttcattt tcaaggaggg ctctcctgga
     1201 aaggacgatc ctcctctttc tccggtccct ttcgatgacc ttggcgctga aggtatcgtc
     1261 gaccagtctc gaggggttga tccccctccc ctcctcggcg aggtgagaga cgggaatgaa
     1321 tccctcaata ccgcagcagt ccactatcag gccgcccttg accttccgga gccccctgac
     1381 ctccaggaca tcatcaccgg taaggctcga ttccattgcg tcccaccggt cgtcgaactc
     1441 cgttctccag cggctcacga tgagctgagc ctcctcgccc tgtttcaggg agacgacctg
     1501 caccctgacg gtatcgccca ctgacgggac atccttggct tccacaaggg acctgtggct
     1561 ccattcgttc cttggtagaa agccttcaca cttgaaaccc acgtcaacca gccatccgtc
     1621 ttcgttggac tccaccacgg tcccctcaac ggtctttccc cgatggatcg ctccaacttc
     1681 gtacttttcg agaaaacttt ccatcgattc atttccttcg ctagaaagcg cggtactgtc
     1741 gttactgttg atcgttctgt tctcttctgt cattaccatc catcctccct gccgctcttt
     1801 gcagacgcaa gtctcgtagc gattctgtgg atcagccagt cgggtgtgct tgcgcccgcc
     1861 gcgatcccga tccttctctt gtcctccaac catccccttt cgagcccctc ttcatcctcg
     1921 atccagaggg tcggtgttcc tgatgtattt gctatctcga agagtttttc cgtgttggcg
     1981 ctgttctttc cgccaatgat gataatacca tctaccgagc ccgcaaggcg tgccaccgac
     2041 ccctgcctcg aagaggtggc gtcacagatg gtgttgaaga ccctcgtttc ggacgccctg
     2101 gccgatacat tggataccag cgcggagagg tcctccttcc gctgggtggt ctgggataca
     2161 atgccgactt ttttcaaccc ttcaatctcg aaactcttta gccgtgaagg atcgacaact
     2221 gtcaccggcc cttccgagga
//
LOCUS       DAOPKQ010000229         2191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_58157_length_2191_cov_5.134831, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000229 DAOPKQ010000000
VERSION     DAOPKQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58157_length_2191_cov_5.134831"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..2061
                     /gene="typA"
                     /locus_tag="PK146_07055"
     CDS             187..2061
                     /gene="typA"
                     /locus_tag="PK146_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPE92032.1"
                     /translation="MHEVEKIRNLAIIAHIDHGKTTLIDSIFRAARVFRENARIEERV
                     MDSNELERERGITIRSKHCTVTWGDHLINLVDTPGHADFSGEVERVLSTVDSVLLLVD
                     ANEGPMPQTRYVLRHALAMGLRPLVFINKADRPGADLAETLNRTFDLFIELGATDEQA
                     DFPVIYGSGLQGWAVKDMGETAKPGDMKALFDMIIEYVPAPEADPEAPFLMQVTTLAW
                     NEYSGRIGCGKVLQGSLRQGEEFIRTTSRWFDPRKQEGDWEVTDSSREKAAQMWITRG
                     LDRVPVEEVNAGDIIWISGPKEIGIGDVFSSPVIEDPAMKPLSIEEPTISMFILVNNG
                     PFAGQEGQAITLRQIRSRIERETRMNIALHMEDLGRPDGIKISGRGELHLAILIEEMR
                     REGAEFCVSRPEVITEMIDGRLFEPMESLIIDVPEEYQGVVFEKIARRKGRMLGLLNP
                     SGGNTLRMEFSIPTRGLIGYRGEFLTDTRGMGIMSSSFNGYGPWTGEINPRNRGSVVS
                     LETGEATAYQIENLQERCTLFISPMDRVYEGMVVGENSRPGDMVCNPTKRKQMTNHRS
                     STQDQYTKLDTPRKMTLEKALEWIASDELVEVTPLSVRIRKAILDDNKRKKRLHREVS
                     "
BASE COUNT          545 a          597 c          640 g          409 t
ORIGIN      
        1 tattcaatat atccatgggt tctccagaag aagtttaata tccagatccc cggggggcaa
       61 gtttttccag gcccgggatc caaataatgg catattgcct gaacagcccc gaagatgtat
      121 cattcccagc ctgtgcacct tttttcaggg aacgcccaga accttttttc aggagatggc
      181 agaaaaatgc acgaagtcga aaagatccgc aaccttgcga taattgccca catcgaccac
      241 ggaaagacca cccttatcga ttctatcttc cgcgctgcca gggtcttccg cgagaacgcc
      301 cgcattgagg aacgagtaat ggacagcaat gagctggaga gggagcgggg cataaccatt
      361 cgctcaaaac actgtacagt aacctggggt gatcacctga tcaaccttgt ggatacccct
      421 ggacatgctg acttttccgg ggaagtggag agggtccttt cgaccgtcga ttctgtgctt
      481 ctgctggtgg atgccaacga gggtcccatg ccgcagaccc ggtatgtact ccgccacgcc
      541 ctggcaatgg ggctgaggcc gctggtgttc ataaacaagg cggatcgccc tggagcagac
      601 ctggcagaaa ccctgaacag gaccttcgac ctcttcatcg aactgggggc aacggacgag
      661 caggctgatt tccctgtcat ctacggatcg gggctgcagg gctgggcggt gaaagatatg
      721 ggagagaccg caaagccagg agatatgaaa gctcttttcg acatgatcat cgaatacgta
      781 cccgctcccg aggctgatcc ggaggccccg ttcctgatgc aggtgaccac cctcgcctgg
      841 aatgaatact ccggccgtat cggatgcggc aaggtactcc agggttccct tagacagggt
      901 gaagagttca tcaggaccac ctcacgctgg ttcgacccca ggaagcagga gggggactgg
      961 gaggtaaccg acagcagccg ggaaaaggca gcacagatgt ggataacaag ggggctggac
     1021 cgggtccctg tcgaggaagt taacgccggg gatataatat ggatatcggg accgaaggag
     1081 atcggcatcg gggacgtctt ctcttcccct gtgatcgaag acccggcgat gaaacccctc
     1141 tccatcgaag agcccaccat atcgatgttc atccttgtca acaacggtcc cttcgcaggc
     1201 caggagggcc aggccataac cctgagacag atccgctcca ggatcgaaag agagaccagg
     1261 atgaacatag ccctccatat ggaggacctg gggaggcccg acgggatcaa gatctcaggc
     1321 agaggagagc ttcatcttgc catactgatc gaggagatga gacgtgaggg tgccgagttc
     1381 tgcgtatccc gacctgaagt cataacagag atgatcgacg gccggctctt cgagccaatg
     1441 gaatccctga tcatagatgt tcccgaggag taccagggag tggtcttcga aaagattgcc
     1501 agaagaaagg ggcgcatgct cggcctcctg aacccttcag gcgggaatac gctccggatg
     1561 gagttctcga tccccaccag gggtctcatc ggctaccggg gggagttcct gaccgatacc
     1621 aggggaatgg gcatcatgtc ttcgtccttc aacggatacg gtccgtggac gggggagata
     1681 aatcccagga acaggggttc cgtggtcagc ctcgaaactg gagaagccac ggcttaccag
     1741 atcgaaaacc tgcaggagcg ttgtacccta ttcataagcc ccatggacag ggtctacgag
     1801 ggcatggttg tgggcgagaa ctcccgcccc ggggacatgg tctgcaaccc cacgaagaga
     1861 aagcagatga ccaaccaccg ctcatccacc caggatcagt acacaaaact ggacacccca
     1921 agaaagatga cccttgaaaa ggccctggag tggatagcct ctgatgagct cgtcgaggtg
     1981 acccccctgt ccgtcaggat caggaaggca attctcgatg acaacaagag gaagaagagg
     2041 ctgcacaggg aggtgtcctg atcacggccg gggaaatgaa ggacggccgc tccttgagta
     2101 gcggccgtca cctgtttcca tggtgcggag ggggagactt gaactcccac agcccgtaag
     2161 ccactaggcc ctcaacctag cgcgtctacc t
//
LOCUS       DAOPKQ010000230         2163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_59200_length_2163_cov_4.779412, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000230 DAOPKQ010000000
VERSION     DAOPKQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59200_length_2163_cov_4.779412"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PK146_07060"
     CDS             complement(<1..1444)
                     /locus_tag="PK146_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPE92033.1"
                     /translation="MRRLTLIMAAIAVLLTLALSSAASAGVVLVLSGGGTRGLAHIGV
                     LKVLEERNIPIDGIVGTSIGSIVGGLAACGYSSSELDEMIVTLDLASLLYDSKGESTV
                     PPGEDASAYIQSILRLEFNERGHITGPLGGLSGKKLLERLQQWSARCPDPDFFQLPIP
                     FAAVATDIVTGEAVVLRRGNLASAMRASMAIPGLFSPWTIDGRLLVDGGLVSNAPVLI
                     ARELFPGRPLVLVDVTGRGKDREDIRTVVDIVDQMITIMTQRNVQEEIGFADLVITPD
                     VGDLPMLEMAEHKEIVTAGEKAAWACLGLIEEVASRDRSSPGIVPSIPAVNVADIQVR
                     GLGPHLSEDVRKRHVGWIGRPADPSEIIQVCTDLRARDDVRTADFWLEHRQDGTAVVV
                     LNVEKEPVWEVAARGYASNLNPYSSLYLDITRRDLLSEGDSLRTHLGLGEHWRFGSRY
                     LSPVREGYRRWEISAKAGSRRLTPEGSDPLE"
     gene            1711..>2163
                     /locus_tag="PK146_07065"
     CDS             1711..>2163
                     /locus_tag="PK146_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPE92034.1"
                     /translation="MVEKKKLPLIWKIAIGFVAGILAGIVFGEKISFIAPIGTIFVAL
                     LQMLVVPLVFASLVVGTASIGDPLSLGRVGVKTIALYLFTTAIAIAIGLTLGNVISPG
                     VGMGLGADLSGFQVPTPPKIGEVIIGLFPRNPVASMAQANMLQIIVFAL"
BASE COUNT          468 a          632 c          590 g          473 t
ORIGIN      
        1 actcaagggg atccgaacct tcaggggtca gtcttctgct gcctgccttc gcgctgatct
       61 cccacctcct gtaaccctcc ctcaccggtg aaaggtatct cgaaccgaag cgccaatgtt
      121 cacccaggcc cagatgagta cggagggaat ctccctcgga aaggagatcg cggcgggtaa
      181 tgtccaggta gagggaagag taggggttaa ggttgctggc ataacccctt gccgcaacct
      241 cccatacagg ttccttctct acgttcagga ccactactgc ggtcccgtcc tgtctgtgtt
      301 ccagccagaa gtcggccgtc cggacgtcat ccctggccct gagatcagta cagacctgga
      361 ttatctccga cgggtccgct ggtctgccga tccagccgac gtgacgcttg cgtacgtcct
      421 cggagagatg ggggcccagc cccctgacct gtatatcggc gacgtttacg gcaggtatcg
      481 aaggaacgat cccgggagag ctcctgtccc tggaagcgac ctcctcaata aggccgaggc
      541 aggcccaggc tgctttctct ccggcagtca ctatctcctt atgctccgcc atctcgagca
      601 tgggcagatc cccaacgtca ggtgtgatca ccaggtccgc gaacccgatc tcctcctgga
      661 cgttgcgttg ggtcattatg gtgatcatct ggtccacgat gtccaccacg gtcctgatgt
      721 cctccctgtc cttccctcta cctgtaacat ctaccaggac aagagggcgt ccagggaaaa
      781 gctcccgggc gatcagcacc ggggcgttgg aaacaaggcc cccgtcaacg aggagccttc
      841 cgtcaatggt ccagggagag aagagtcccg ggatcgccat ggaggctcgc atggccgatg
      901 caaggttgcc tctccggagg acaactgcct cgccggtcac tatgtccgtt gctactgcgg
      961 caaagggtat gggtaactgg aagaaatcgg gatcggggca tcgtgcgctc cactgctgaa
     1021 gtctctccag gagcttcttc ccgctcagcc cccccagggg gccggtgatg tgccccctct
     1081 cgttgaattc aagccggagg atggactgga tgtaggcgga agcatcctct cccggaggga
     1141 cagtggattc ccccttgctg tcatagagga gggaggcgag atcgagggtc actatcattt
     1201 cgtcaagttc ggaggaggag taaccgcagg ctgcgagacc tcccacaatg gatccgatgc
     1261 ttgttccaac gatgccgtct atgggaatgt tccgttcctc gagcaccttg agaacgccga
     1321 tgtgggcgag acccctcgtc ccccctccgg agaggacaag gaccactcct gctgaagccg
     1381 ccgacgacag ggcaagggtc aaaagaacag caattgctgc cattattaaa gttagcctgc
     1441 gcaactggtc accctccttg caagaattga acttccacat acctgcaatg aatttttatc
     1501 ttaccagatt aaagctctca tcaaggtcat attggggaca aaggagtaga aaaacgatat
     1561 aatccaagtc tggacagcat gtatttttaa cttactctcc cgcattttct gaattatccc
     1621 ctgggggtga tatgagcaga attacaacag tgtgacaaga gcgaaagaga aaggcaaaac
     1681 ccgtgaacaa ttgaaaggag tgggatcaag ttggtcgaaa agaagaaact tccccttatc
     1741 tggaagattg cgatcggttt tgtggccggc atcctggccg gaattgtctt cggagaaaag
     1801 atctctttca ttgctcccat cggaacaatc tttgttgcac tgctgcagat gctcgtcgtt
     1861 cccctggtct tcgccagcct ggtggtcggt acggcctcca tcggcgaccc gctgtccctg
     1921 ggcagggtcg gggtcaagac catagccctc tatctcttca caaccgccat tgccattgcc
     1981 ataggtctta cccttggaaa cgtcatcagc cccggtgtag gtatgggtct gggggccgac
     2041 ctttcgggat tccaggttcc gaccccacca aagatcgggg aggtcataat tggtctcttc
     2101 cccaggaacc ccgtggcatc catggcacag gccaacatgc ttcagatcat cgtcttcgcc
     2161 ctg
//
LOCUS       DAOPKQ010000231         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_59266_length_2162_cov_3.376364, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000231 DAOPKQ010000000
VERSION     DAOPKQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59266_length_2162_cov_3.376364"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PK146_07070"
     CDS             <1..229
                     /locus_tag="PK146_07070"
                     /inference="COORDINATES: protein motif:HMM:NF023785.2"
                     /note="catalyzes the coenzyme A-dependent decarboxylation
                     of 2-oxoacids, such as pyruvate and 2-oxoglutarate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid ferredoxin oxidoreductase"
                     /protein_id="HPE92035.1"
                     /translation="KKNTFDLSEDHDPSDLKSALERALEGPPYPLGVIYQTEGRPTFE
                     DSMGCYHRDDTPLCRRAYGPERVEDLIASFQ"
     gene            270..623
                     /locus_tag="PK146_07075"
     CDS             270..623
                     /locus_tag="PK146_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92036.1"
                     /translation="MRKKTSMITILTVLFLVAASYAAAHPPTSIELDYSRDDGILGIW
                     IGHVVGNTSSHFIEEVEVKVGGREAAELRYASQGDRSGERIVVTIGRFAPGTRIEVKA
                     ECNRSGNMTGVLVVE"
     gene            complement(681..1397)
                     /locus_tag="PK146_07080"
     CDS             complement(681..1397)
                     /locus_tag="PK146_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92037.1"
                     /translation="MIILMLCVLGATVPASAQHLKVLTVPGHPVSLILETSEGIITSA
                     LLRSPAGIQKILPLEGFAYAGETYTEPYADGDFRKDLLWTITFTRPGDRSRGLYLWIG
                     VTTQIQRAWVIISPLGQTYWDTIPMKIYAPRGTALFVSPNLPAYDDLPQFGGNRTLTF
                     VYTIALTPEGPNFQPVPEVYRQLYRITATIRDAEQITERREAYSRLLEDYEALSRGGK
                     PSTEVIQNFTWKRILYLDWK"
     gene            complement(1474..1956)
                     /gene="folK"
                     /locus_tag="PK146_07085"
     CDS             complement(1474..1956)
                     /gene="folK"
                     /locus_tag="PK146_07085"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPE92038.1"
                     /translation="MLAGISLGSNIGDRLGTIRQASLLLKGMGIQIVASSDIFETAPW
                     GVKEQPWFLNACLLVDTSVSPRELLSRLQSIENRLGRVSRFRWGPREIDLDLLFMDGL
                     AVDEEDLVLPHPEMHNRSFVLVPLNQVAPDWVHPLLHRRVRELLGQVSVEEVLRITDF
                     "
     gene            complement(1956..>2162)
                     /locus_tag="PK146_07090"
     CDS             complement(1956..>2162)
                     /locus_tag="PK146_07090"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPE92039.1"
                     /translation="ARPLLIGHSRKSTIGKVLDLENPEDRLEGTLALSAFCAMKEVAL
                     IRVHDVAENIRAVRMIGALKGEGI"
BASE COUNT          479 a          589 c          647 g          447 t
ORIGIN      
        1 caaaaagaac acattcgacc tgtcggaaga tcatgacccc tcggacctta agtctgcctt
       61 ggagcgggcc ctggaggggc cgccctaccc cctgggggtc atctatcaga ccgagggaag
      121 gcccactttc gaggattcca tgggctgtta tcaccgtgat gacactccac tctgcaggag
      181 ggcctacgga ccggagaggg tggaggatct tatagcctcc ttccagtaaa ggaccgggtg
      241 gtaaaaattc agcagaaagg tgaacaccta tgagaaaaaa gacttcgatg ataacgatcc
      301 tgacggtact ttttcttgtt gctgcctcat acgccgcagc tcaccccccg acatcgatag
      361 aactggatta ttcccgggat gacggaatac tgggaatatg gatcggccat gtcgtcggca
      421 acacttcctc tcacttcatt gaggaggtgg aggtcaaggt cgggggaagg gaagcggcgg
      481 agctcaggta cgcaagccag ggcgacagga gcggggaaag gatagtagtg acgataggca
      541 ggttcgcacc cgggacccgg atcgaggtaa aggctgagtg caaccgctca ggtaacatga
      601 caggcgttct ggtcgtggag tgagtgccga aggacctgtc ggcaatcagc gaaagagaac
      661 ggccgccact ggttcatttc tcatttccag tcaaggtaaa ggatgcgttt ccaggtgaag
      721 ttctggatca cttctgtgga gggtttcccc cccctggaga gggcctcgta gtcctccagc
      781 agcctggagt aggcctcccg gcgctcggtt atctgctctg cgtccctgat ggtcgccgtg
      841 atccggtaga gctggcggta aacctcggga acgggctgga agttaggtcc ctcaggtgta
      901 agggcgatag tgtagacgaa ggtcagggtc ctgttccctc cgaactgggg gaggtcatca
      961 taggcaggca ggttagggga gacgaaaagg gccgttcctc ttggggcgta gatcttcatc
     1021 gggatcgtgt cccagtaggt ctgtcccagg ggactgatga tgacccaggc cctctggatc
     1081 tgggtggtga caccgatcca gaggtataac cccctggatc ggtcgccagg ccttgtaaag
     1141 gtgatggtcc agaggagatc cttccggaaa tccccatcgg cgtatggttc ggtgtaggtc
     1201 tcaccggcgt aggcaaaacc ctcaaggggg aggatcttct gtatccctgc gggggatctg
     1261 agaagcgccg aagtaattat cccctcgctg gtctcaagga tgaggcttac agggtgacca
     1321 gggaccgtaa ggactttcag gtgctgcgcc gatgccggca ccgtggcacc aagaacgcaa
     1381 agcatcagta taatcaaggc ggccaggtac cggacaggga caaagtatcc aggcttgctc
     1441 ggtctcaaag gcgtcactcc ccaaaatggt agttcaaaag tctgttatcc tcagaacctc
     1501 ttcgacagag acctgtccga gcagctccct caccctcctg tgcagaaggg ggtggaccca
     1561 gtcgggtgct acctggttca gagggaccag tacgaaggac ctgttatgca tctccgggtg
     1621 aggcaggacc aggtcttctt cgtccacggc aagaccatcc atgaagagga ggtcaagatc
     1681 tatctccctg ggaccccacc tgaaccttga gacccgacca aggcggtttt cgatggattg
     1741 cagcctggag agcagttccc tgggcgagac agaggtatcc accaggaggc acgcgttcag
     1801 gaaccagggc tgttccttca ccccccaggg ggctgtctcg aaaatgtcgc tggaagcgac
     1861 gatctggatg cccatgcctt ttaacaaaag tgaagcctgc cggatggtgc ccagcctgtc
     1921 accaatattg cttccaaggg agatacctgc tagcatcaga tcccttcccc tttcagagcc
     1981 ccgatcatcc ttacggccct tatattctct gccacatcgt gcacccttat cagggccacc
     2041 tccttcatgg cacagaaagc tgacagggcg agggtcccct ccagcctgtc ctcagggttt
     2101 tccagatcca gtaccttccc gatggtgctc ttccgggagt ggcctatgag cagtggcctt
     2161 gc
//
LOCUS       DAOPKQ010000232         2147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_59845_length_2147_cov_4.420172, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000232 DAOPKQ010000000
VERSION     DAOPKQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59845_length_2147_cov_4.420172"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="PK146_07095"
     CDS             <1..1344
                     /locus_tag="PK146_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HPE92040.1"
                     /translation="IFLLGTDVGILPMGHSIGAALVHRRNVPLILATGFFIGFFITIA
                     EPDVRVLASQIAWVAPSISQPLLLVTIALGVGFFVSVGLGRMLLRIPYYFLLIGFYLV
                     VFALAYFSAPRYLSIAFDAGGATTGPMTVPFIIALGVGVAAVRGTKEAESDSFGLVGL
                     ASIGPILAVLSMGVMDRGGDAGVSAAGAMGTSEGLAAAFTSILFETGHEAFMALTPIA
                     AILVVFQLVLLHMNRYQVIRMIKGLIYTFVGLVVFLVGVKGGFLPAGSVIGGMIGASP
                     FNWVLIPIGFVLGAVVVLAEPAVWVLNDQIEEVSGGHINKRFMLIAFSIGVAIAVGIS
                     MYRVLSGVSIWWFLIPGYGIALAMTFFCPPLFTAIAFDSGGVASGPMSSTFILAFTLG
                     ASVSSGGNPLTDAFGVVAMIAMVPLITIQALGLVFKRKEQEVERGFEERIEKEVA"
     gene            1344..2027
                     /locus_tag="PK146_07100"
     CDS             1344..2027
                     /locus_tag="PK146_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92041.1"
                     /translation="MTKNSEVHGSCGKLLLTIVKEGLGDRVVEITRKAGAKGGTLLPG
                     RGRSENPVAGFFCIGDFREEIVLTLVRGEDVSNVMNALRAFGGTAGKHREGISVQLDV
                     SQILHRAGKGSATCDLLERSGPMEIKTEQIVISIIANRGCADDIMSVARKAGASGGTI
                     LNARGTASEKDVKFLGVPLFPEKEVLLILAETARSATIFNALKECDCITRPGGGIAFS
                     IAVEDFVWF"
BASE COUNT          440 a          535 c          648 g          524 t
ORIGIN      
        1 attttccttc tgggaactga cgtgggcatc ctgcccatgg ggcacagcat cggggcggcc
       61 ctggttcaca gaaggaatgt gccgctcatt ctggccactg ggttcttcat cgggttcttc
      121 ataaccatcg ctgagcctga tgtaagggtt ctcgccagcc agatcgcctg ggtggcccct
      181 tcgatatcac agcctcttct tcttgtcact atcgcccttg gagtgggttt cttcgtatcc
      241 gtcggtctgg gcaggatgct cctgagaata ccctattact ttctccttat tggattctat
      301 ctggtggtct tcgctcttgc ctacttctcg gcgccacggt acctgagtat tgcctttgac
      361 gcaggtggcg ccactacagg acccatgacg gtacctttca taattgctct gggagtaggc
      421 gtcgccgcgg taaggggaac caaagaggcc gaaagcgaca gtttcggcct ggtgggactt
      481 gcctccatag gcccgatcct tgccgtcctt tccatgggtg taatggacag aggcggagat
      541 gccggtgttt ctgccgccgg ggccatgggg acatcggaag gactggctgc agcgttcaca
      601 agtatccttt tcgagacagg tcatgaagca ttcatggccc tcactcctat cgcggcaata
      661 cttgtggttt tccagctggt gctccttcac atgaaccggt accaggtgat aaggatgatc
      721 aaaggcctta tctatacgtt tgtgggtctg gttgtattcc ttgtcggggt aaagggaggg
      781 tttctccccg ccggttcggt gattggtggc atgatcgggg catcaccctt caactgggtc
      841 cttatcccga tagggtttgt gctcggggcg gtcgtggtcc tggccgaacc cgcagtatgg
      901 gtcctcaacg accagataga agaagtctcc ggcggtcata tcaacaaaag gttcatgctg
      961 atagcctttt cgatcggggt cgcgatcgcc gtgggcatct cgatgtaccg ggtgctctcc
     1021 ggggtcagta tctggtggtt tctgatcccc ggctacggga tcgccctggc aatgactttt
     1081 ttctgtccgc cgctcttcac ggccattgca tttgattcag gcggggtcgc ctcgggaccg
     1141 atgtcttcga cctttatcct ggcctttact ctgggtgcct cggtttcctc gggaggcaac
     1201 cccctgaccg atgctttcgg tgtggtggca atgatcgcaa tggtccctct gatcaccatc
     1261 caggcacttg gacttgtgtt caaacgtaaa gagcaagagg ttgaaagggg tttcgaggaa
     1321 aggatagaga aggaggtcgc ttgatgacga aaaattcgga ggtgcatgga tcctgcggaa
     1381 agcttcttct cacaatagta aaagaaggtc tgggcgacag ggttgttgag attacccgga
     1441 aagccggggc aaaaggcggg actctcttgc ctggaagggg gagatcggag aaccctgtcg
     1501 ccggattctt ttgtattgga gatttcaggg aggagatcgt gctgaccctg gtaagagggg
     1561 aagatgtctc caacgtgatg aatgccctta gggcctttgg cggaactgcg gggaaacaca
     1621 gagaggggat ttcagttcag ctggatgtat cgcagatact ccatcgagcg gggaaaggtt
     1681 cagccacctg tgatcttctg gaaaggagcg gacctatgga aatcaagacc gagcagatag
     1741 tgataagcat tatcgcgaac agggggtgtg ccgacgacat aatgtctgtc gcccgcaagg
     1801 ccggagcctc gggggggact atcctcaatg ccaggggaac ggccagcgag aaagacgtga
     1861 aattcctcgg ggttccgctc tttccggaga aggaggtcct gctgatcctt gctgagacag
     1921 cacgttcggc aaccatcttc aatgccctga aggagtgcga ctgcattact cgacccggcg
     1981 gggggatagc tttctccatc gcggtggagg atttcgtctg gttctagaac gaaccttaag
     2041 ggagcctcat aggcggaatc tcataggtga catgaacttc gtaggtgtca gacctacacg
     2101 gcctgttgcc caaaaggcga aaatgaaggc gaaagaatcg atcatag
//
LOCUS       DAOPKQ010000233         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_60076_length_2141_cov_3.488495, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000233 DAOPKQ010000000
VERSION     DAOPKQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60076_length_2141_cov_3.488495"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1400
                     /gene="murA"
                     /locus_tag="PK146_07105"
     CDS             117..1400
                     /gene="murA"
                     /locus_tag="PK146_07105"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPE92042.1"
                     /translation="MNYKMLIHGGKPLEGSIIVQGAKNAALPVIASSLLLRDRTLRLK
                     RVPKLQDIFTMADLLRHLGASIHFKDNEMSIFVPDELNWETPKSLVRKMRASSLVLGP
                     LLARTGRAILPLPGGCAIGSRPIDLHLKGLVKLGANIDLVHGAVHASADGLKGAKIYL
                     DFPSVGATENLLMAAVFAEGETVIENAAREPEIRNLIETLNLMGCSIEGAGTGTLHVR
                     GTGDLDGTGIEIIPDRIEASTYLIAGAATRGDVTVTRVIPEHLDAITAKLEEAGVGIE
                     TGEDSVRVFNRAPMKSVSLKTLPYPGFPTDVQPQFTAFMATTEGTSIIQESVFESRFL
                     HVSELKRMGANVELQGNTAVVTGVPCLNGADVRATDLRAGAALVIAGLSSSDETSLFG
                     LGHIWRGYEAMDEKIRSLGGQVKIVVSEENGDHKW"
     gene            1431..>2141
                     /locus_tag="PK146_07110"
     CDS             1431..>2141
                     /locus_tag="PK146_07110"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPE92043.1"
                     /translation="MWLRNVIRNDLAGVDKYRYGKPVSELQRELGLERVVRLSSNENP
                     WPLPGSVLNAIQTSLENLNRYPDPQSHRLRKLLAQKWGVSTGEVIIGAGTEGILHSLF
                     HSIIEFGDEIVCPVPTYPLYRVAAVASGARFIEVPACEGGTWEIGNIIRACTERTKAV
                     ILSNPCNPLGTIMERERMLNLAHELDSRQVLLIVDEAYAEYVEDPGYLSGIDLFRDIG
                     RIVITRTFSKIYGLASLRV"
BASE COUNT          509 a          559 c          635 g          438 t
ORIGIN      
        1 cagggagggc gtctgacagg atcgtagaga ctatcgagag cctgttcctg aaagagatga
       61 gataatgaaa agaaacgacc gtcaggctga tagaattttc cttgtggggt gatcatttga
      121 actacaagat gctgatccac gggggcaaac cccttgaagg gtccataata gtacagggag
      181 ccaaaaacgc ggccctgccg gtgatcgcgt catccctgct cctgagggac agaacgctgc
      241 gcctgaaaag ggttcccaaa cttcaggaca ttttcaccat ggccgatctt ttgagacacc
      301 ttggtgcgag tatccatttc aaggataacg agatgagcat ttttgtcccc gatgaactta
      361 actgggagac cccgaaatcg ctggtaagga agatgcgtgc atcttccctg gtattgggtc
      421 cgcttctggc caggaccggg agggcgatcc ttccccttcc cggaggttgc gccataggaa
      481 gcaggcctat cgatcttcac ctaaaggggc ttgtcaagct cggggcgaac atcgacctcg
      541 tacacggtgc ggtccatgcc agtgccgacg gcttgaaagg tgcgaagatc tacctggact
      601 tcccctccgt cggagccacg gagaaccttc tcatggccgc ggtctttgcg gaaggggaga
      661 ccgtcatcga gaatgccgct cgtgagcctg agataaggaa cctgatagaa acgcttaacc
      721 tcatgggttg ttccatagaa ggagccggga caggcactct tcacgtaaga ggcaccggtg
      781 accttgacgg aaccgggatc gagataatac ccgacaggat cgaggccagc acctatctaa
      841 tcgccggagc cgccacaaga ggtgacgtaa cggtcacccg tgtcatcccg gagcatctcg
      901 atgccattac ggcgaagctc gaggaagctg gagtcgggat agagacggga gaggacagcg
      961 tcagggtttt caacagggcc cccatgaaga gtgtctccct gaagaccctt ccatacccgg
     1021 gtttccctac cgacgtccag ccccagttca cagccttcat ggccaccacc gagggtacca
     1081 gcatcattca ggagagtgtc tttgaatccc gtttcctcca cgtgagcgaa ctcaagagga
     1141 tgggtgccaa tgttgaactc caggggaaca cggcagttgt caccggtgtc ccctgcctta
     1201 acggagccga tgtgagggcc acggacctga gggctggtgc tgcccttgtg atcgccgggc
     1261 tatcttcttc agatgagacc tccctcttcg gccttggtca tatctggcgg ggttatgagg
     1321 ctatggacga gaagatcagg tcccttgggg gtcaggtcaa gatcgtcgta tccgaggaga
     1381 acggagacca taagtggtga tcaaataata gagacgggga atgagaattg gtgtggctaa
     1441 gaaatgttat caggaacgat ctggcaggtg tggataaata ccggtacggg aaacccgtga
     1501 gtgagcttca gagagagctg ggattggaga gagtggttcg cctgagctcc aacgagaacc
     1561 cctggcccct cccgggatcg gtattaaatg cgatccagac cagtctggag aacttgaacc
     1621 gttatcccga cccccaatcc cacagactca ggaagcttct tgcccagaag tggggagttt
     1681 cgacggggga ggtcataatc ggggccggaa ctgaagggat cctgcactcc ctcttccatt
     1741 cgataataga gttcggcgat gagatcgtct gccctgtgcc gacctatccc ctctacagag
     1801 tggcggccgt cgcctcagga gcacggttca tcgaggtacc tgcctgcgaa ggcgggacct
     1861 gggagatcgg gaatataatc agggcctgta cggaaaggac caaggcagta atactgtcca
     1921 acccctgcaa ccccctcgga acgataatgg agagggagag aatgctcaac ctggctcatg
     1981 agctggattc caggcaggtc ctgctgatcg ttgacgaggc ctatgccgag tacgtggagg
     2041 accctgggta cctctcggga atagacctgt tcagggatat cggaaggata gtaattaccc
     2101 ggaccttctc caagatctac gggcttgcat ctctgcgtgt a
//
LOCUS       DAOPKQ010000234         2136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_60236_length_2136_cov_4.521384, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000234 DAOPKQ010000000
VERSION     DAOPKQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60236_length_2136_cov_4.521384"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK146_07115"
     CDS             <1..212
                     /locus_tag="PK146_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92044.1"
                     /translation="SLYRDVRFLYILELKLDGRVVFLVDSEPEGSPDESLPGDLLDKP
                     TPPLLNAFLTGKGNIEGPLEDSWGR"
     gene            270..>2136
                     /locus_tag="PK146_07120"
     CDS             270..>2136
                     /locus_tag="PK146_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE92045.1"
                     /translation="MDINTDLWTASVRRTNKPYQAFSTLLGILGLFGLFLFTWKVRSR
                     RATSKQGILLRYAEPLMVFAAGILVTFLLAYTADIHERLDRRETFTRLASKRISNIRD
                     TLSDIRNFHLEGLARFFEGSEIVSPSEFETFTEHLSREEAVRAWAWIPVVPHGERDHF
                     ELLMGTTGSSSPVFTEKGEEGLLKAGERDFFYPVLYLTPLHGNEVLQGFDLGSDPVLL
                     EALTAGSGQDCFSVAGPVRLFADEGSKGELLFLRPIYSEGQNGTSGFVMAICSPEKTL
                     LLCSGKGSLTDFSYLDLQLLLLEDDGKEVFLASTEGPGLVRMEEHTLSSLEGGFEQAV
                     PIFAMGKTFVATATPNEAFSDLYPRRAGWITAIAGILVALTLFFASLTEVRRRTGLEE
                     KVRERSLALAQSEHRYRSLFENSHSVMLIIDPTDGSIIDANPAAELFYGWPREEIIRM
                     KISSINTLSPEEVQEEMGKARKKGTTRLFFQHRLSNGSVREVEVFSGPIMIDNKALLF
                     SIVHDITDRRVAERALEESENRYDSFINTHRDIIFVKDDQLRYVVVNEAAKRFFGRER
                     EEILGLTDSELMDPADARHCYESDMKALMKGSAITIEERVGNRVFETTKFPLPLED"
BASE COUNT          476 a          589 c          581 g          490 t
ORIGIN      
        1 ggtcccttta ccgcgacgta aggttcctct acatcctcga actcaaactc gacggaaggg
       61 tggtcttcct tgtcgattcc gagccggagg gttcacccga cgagtccctg cccggagatc
      121 ttctggacaa acccaccccc ccgctcctga acgccttttt gacgggcaag ggcaatatcg
      181 agggccccct ggaggactcc tggggtagat gaatctccgc caacgtccca cttcacgaca
      241 ctaccacggg gaggatcttc ctccttggaa tggatatcaa taccgatctg tggaccgcct
      301 ctgtccggag gaccaacaag ccctaccagg ccttttcgac cctcctcggt atcctgggtt
      361 tattcggact gtttctcttc acctggaagg tgcgttcccg gagagcaacc agcaaacagg
      421 gtattttgct tcgttacgcc gagccgctga tggtatttgc agccggtatc cttgtgacct
      481 ttcttttggc ctacacggcg gacatccatg agaggcttga caggagagag accttcacca
      541 ggctcgcctc gaagaggatc tcgaacattc gggataccct gtcggacatc aggaattttc
      601 accttgaagg tcttgccagg ttcttcgagg ggagcgagat cgtcagccct tccgaattcg
      661 agaccttcac cgaacatctt tcccgtgaag aagccgtcag ggcctgggct tggatacccg
      721 tcgttcctca tggcgagagg gatcatttcg aactgttgat gggaactacc ggctcctcaa
      781 gtcctgtttt cacggaaaag ggggaagaag gacttctgaa agccggtgag cgggacttct
      841 tctatccggt gctttatttg acacccctcc atggtaatga agtccttcag ggttttgacc
      901 tgggatccga tccggtcctc cttgaagcat tgaccgccgg ctccggacag gactgttttt
      961 ctgtcgccgg tcccgtcagg ctgtttgccg atgaaggatc gaagggagaa cttctgtttt
     1021 tgaggcccat ttattctgag gggcagaacg gcacatccgg tttcgtaatg gcaatatgct
     1081 ctccagaaaa gacgctcctt ctttgttctg ggaagggttc cttgacggac ttctcctacc
     1141 tggacctcca gctcttactt cttgaggacg acggtaagga agtttttctt gcctctacgg
     1201 agggacctgg tctggtcaga atggaggaac acactctctc cagcctcgag ggtggcttcg
     1261 aacaggctgt tcccatattt gccatgggaa agacctttgt cgcgacagcc acacccaacg
     1321 aagcattctc cgacctttac ccgaggaggg caggatggat cactgctatc gcagggattc
     1381 tcgtggccct gaccctgttc ttcgcttccc tgacggaggt cagaaggcgg accggcctgg
     1441 aggagaaggt ccgggaacgg agccttgccc ttgcacagag cgagcatcgt tacaggtctc
     1501 tctttgagaa cagccactca gtcatgctca tcatcgaccc caccgacggg agtatcatcg
     1561 atgccaaccc ggcagcggag ctcttctacg gttggcccag ggaagagatc atccgtatga
     1621 agatctcctc gatcaacact ctctccccgg aagaggtcca ggaggaaatg ggaaaggccc
     1681 ggaaaaaggg gactactcgt ctgtttttcc aacatcgcct ttccaacggt tcggtcaggg
     1741 aagtggaggt tttcagcggc cccataatga ttgacaacaa ggcccttctc ttctccatag
     1801 ttcacgacat aacagaccgc agagtggccg agagggctct tgaggagagc gagaacaggt
     1861 atgactcatt cattaacacc catcgggaca taatcttcgt taaggacgac cagctcaggt
     1921 acgttgtagt gaacgaagca gcaaagagat ttttcgggag ggaaagggaa gagatactgg
     1981 gactgactga cagtgaactg atggaccctg ctgacgccag acactgttac gaatcggaca
     2041 tgaaggccct tatgaaaggc tcagccataa ccatcgagga acgggtcggg aaccgggtgt
     2101 ttgagaccac aaagttcccc ctgcctctgg aggaca
//
LOCUS       DAOPKQ010000235         2103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_61583_length_2103_cov_4.052246, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000235 DAOPKQ010000000
VERSION     DAOPKQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61583_length_2103_cov_4.052246"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PK146_07125"
     CDS             complement(<1..1176)
                     /locus_tag="PK146_07125"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPE92046.1"
                     /translation="MNPEGWVSLLPVTVLLVAVIVTRRALESLLIAIAVGFMLKDGLS
                     FFPSMLDTVYSVMQDPTIGFVIILCSVMGAFVAVLDHSGGTKGFGDWVSSRVSTRRGV
                     LLGTWLMSLFIFIDDFLISLTLGICMTPSADRYKVPREMTAYVVNSSAAPVASLVPIS
                     AWAVFIAALLEKYLALPAGEGLALFVRTIPFNFYAWFAFFMVPLVVTGIVPVIGPMRQ
                     AEERVRRTGELAPPGSELISMRKPHDMSEEGPKAMARNLVLPVLVLIAGTMWFGVNLF
                     KGGVIALSFCCAFYRIQGIMPVARFTETCLRGIGNMLFPMVFTCLAFTFNYVNQELGL
                     IPFIIGKATPLLSPALFPLIIFVILGFVAFLVGISWSLYLIAFPLVIPLAQALGADLV
                     "
     gene            1320..>2103
                     /locus_tag="PK146_07130"
     CDS             1320..>2103
                     /locus_tag="PK146_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPE92047.1"
                     /translation="MGTDARSRLTVLHVTPEMTPVSKVGGLADVAGSLPGALQRLGVD
                     ARVFTPSWPGVAERLRDLGARAISGPDRICVPFGGRKVCSDFSVVDLAGTSLCLLEDD
                     ELFSNPSIYPEKLDPLSAMPFAFLSLAAVEYSLSNEWKPQILHLHDWPSSLVPAALKW
                     HRVYGVAREGPRTVLTIHNIAHQGILPLSALDEWGFGPEALTLDGVEFYGSANLLKGG
                     ITASDAVVTVSPNYAREILSPEFGEGLEGVLEYRKSKITGILN"
BASE COUNT          521 a          580 c          595 g          407 t
ORIGIN      
        1 gaccagatcc gctcccagag cctgtgcgag agggatcacc agcgggaatg cgatgaggta
       61 caggctccag cttatcccca caaggaatgc gacgaaacca aggataacga agatgatcaa
      121 agggaagagg gctggagaga gaagaggtgt agccttcccg atgatgaaag ggatcagccc
      181 cagttcctgg ttaacgtaat tgaaggtgaa ggccagacat gtgaaaacca ttgggaacag
      241 catgttgccg atccccctca ggcaggtctc cgtgaaacgg gcaacgggca tgatgccctg
      301 gatacggtaa aaggcgcaac agaaggatag tgcaatgacc cctcccttga aaaggttgac
      361 accgaaccac atggtccccg ctatcagcac gaggaccggc agtaccagat tgcgggccat
      421 cgccttcggc ccttcttcac tcatatcatg tggttttctc atcgagataa gctccgaacc
      481 tggaggagcc agttcccccg ttctccttac cctttcttcg gcctgtctca tgggcccgat
      541 gacgggaaca atacctgtca ccaccagggg caccatgaaa aaggcgaacc atgcatagaa
      601 attgaagggg atcgtcctta cgaacagggc cagaccttcg ccggcgggaa gggccaggta
      661 cttttcaagc agcgcggcga tgaacaccgc ccaggcagaa attggcacca gcgaagccac
      721 aggagcggcg gaggaattga ccacgtaggc ggtcatctct cttgggacct tgtagcggtc
      781 tgcagaaggg gtcatgcaga tcccgagggt cagactgata aggaaatcat cgatgaatat
      841 gaagaggctc atgagccagg tccccaggag aaccccccgt cgggtggaga ccctggaaga
      901 gacccagtcg ccgaaacctt tggtgccccc tgagtggtcg aggacagcga caaaggcccc
      961 catcactgaa cagaggatta tcacgaagcc gatggtaggg tcctgcatta cactatagac
     1021 ggtgtccagc atggacggga agaaggatag accgtccttt agcatgaacc ccacagctat
     1081 ggcgatcaac agggattcca gggcgcgtct cgtaacgatc acggccacaa gcagaacagt
     1141 gactggcagc agggataccc aaccttcagg attcatatct tcggccccca ataccgggaa
     1201 gaaagaattc ttggtcaaac ctggacgatc atacaccctg ccaggaagtg gcgaagcccc
     1261 tttaatcata gtaaaatagc attagttcaa agaaacacga atatcgggag gagaccccaa
     1321 tgggaacaga tgctcgctcc cgtctgacag tccttcacgt tacccctgag atgacacccg
     1381 tctccaaggt gggtggcctt gccgatgtgg cgggatccct tccgggcgca ctccaaaggc
     1441 ttggagtgga tgcccgtgtg ttcacaccat cgtggccggg ggtggctgaa agactgaggg
     1501 atcttggagc cagggcgata tcaggacctg accggatatg tgtacccttt ggtggccgaa
     1561 aagtctgcag cgatttcagt gtcgtcgatc tggcggggac atccctctgc ctgctggaag
     1621 atgatgaatt gttctccaac ccctcgatct acccggaaaa actcgatcct ctatcggcaa
     1681 tgccctttgc cttcctctct cttgccgcag tggagtactc cctatcgaat gagtggaagc
     1741 cccagatcct gcaccttcat gactggccat cctcccttgt ccccgcagcc cttaaatggc
     1801 acagggttta cggcgtcgcc cgtgagggac cccggaccgt cctgactatc cataacatag
     1861 cccaccaggg catcctcccc ctttctgccc tggatgagtg gggttttggc cctgaggcac
     1921 tcactctgga cggggtagag ttttacggaa gcgccaatct gctgaagggt ggcataaccg
     1981 cttctgatgc tgtggtgacg gtaagcccta attacgccag ggagatcctt tcgcctgagt
     2041 tcggcgaggg gctggaaggg gtcctggaat acaggaaaag caagataaca gggatactca
     2101 acg
//
LOCUS       DAOPKQ010000236         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_61913_length_2095_cov_4.418137, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000236 DAOPKQ010000000
VERSION     DAOPKQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61913_length_2095_cov_4.418137"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..723)
                     /locus_tag="PK146_07135"
     CDS             complement(166..723)
                     /locus_tag="PK146_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPE92048.1"
                     /translation="MWTEPTGDLLEKCLEGESQSLEELLVLLTPLVRHLACRFSRPGD
                     LHFDDLLQEGYISVLRGVHGYDPSRGKFSSFVFSCIRNGMVTFLRREKTQRSIKRLLP
                     PELEVHHQLPAPKIIDLDLLEGLSVAESTVLDAFLDTGSVTSSAKILGWPRKRVDNAL
                     QRARRKLRRKLNGESQTGRGPGEGI"
     gene            complement(739..2061)
                     /locus_tag="PK146_07140"
     CDS             complement(739..2061)
                     /locus_tag="PK146_07140"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPE92049.1"
                     /translation="MNPVAFIESKRDGKEATTSEIRDFVNLAHKGEIPEYQVAAWLMA
                     VFFRGMSGMETRTFTEALSRSGRVINFPPDINPVDKHSTGGVGDKTSLVVVPLAAACG
                     VPVAKLSGRGLGFTGGTVDKLESIPGFSAHLEMGRFIDQVRSTGCAISGHSGDLAPAE
                     ALFYELRDVTGTIPSIPLITSSIVSKKMAGGSRAFVFDVKCGSGAFMEDQLSARELAE
                     SLVDLSASMGRGSMALITDMSQPLGMWVGNAAEVAEAVDLLLGRGPDDLRELSLRLTA
                     SMVHLGRPSISLDEALSLAERSLEEGKGFRKFEEMVRSQGGDLDRFQERVSRGEHLSR
                     TIREISAAESGILTRCDARRVGDAVRFLGGGRLSKKDAVDHGVSCQILRKIGDAVSAE
                     EPLARVFFSGEGVRWEEARAGLVSAFEIGESVTPPKLLLGSVGPREVD"
BASE COUNT          418 a          682 c          540 g          455 t
ORIGIN      
        1 gccgtaccga cccccaccgg gtttgaagcc cggggcactc accagagcac actcgtctcc
       61 gacagccaca atattttaga tcctggctgg ccccatgtcc ataaagaaag ggacctgaag
      121 tctctccaga agatggggcc tgggacacat ctggcgtcag ggacttcaga tcccctctcc
      181 aggtcctctt ccggtttgac tctccccgtt cagcttccgc ctcagcttcc gcctggctct
      241 ctgaagtgcg ttgtccactc tttttctcgg ccagccaagt atctttgccg atgaagtaac
      301 ggatcccgta tccagaaagg cgtccagtac cgtgctctcc gccaccgaaa gaccctccag
      361 cagatccagg tcgatgatct ttggggccgg gagttgatga tgcacctcca gctcaggcgg
      421 caatagcctc ttgatggatc tctgggtctt ttccctccgt aaaaaggtca ccatcccgtt
      481 ccttatgcat gaaaaaacga aggaggagaa cttgccccgg gaagggtcgt atccatgcac
      541 gcccctcagt accgagatat atccctcctg tagcaggtcg tcaaaatgaa gatcgcctgg
      601 tcggctgaac cggcaagcca gatgtcttac cagaggagta aggaggacga ggagttcctc
      661 cagagactga ctttcgccct caaggcattt ttcaagaagg tcccccgtcg gttccgtcca
      721 catatctccg gtcctgccct aatcaacctc cctgggccca actgaaccca ggagcagttt
      781 cgggggggtg acactttcgc cgatctcgaa agcactgaca agaccggctc gagcctcttc
      841 ccaacggacc ccttctccgg agaagaatac ccttgccagc ggttcctccg ccgacaccgc
      901 atcaccgatc ttcctgagga tctggcagga taccccgtgg tctactgcat ctttctttga
      961 gagccgcccc cctcccagga acctgacggc atcgcccacc cgtcttgcat cgcatctggt
     1021 caggattccg ctctcagcgg cggagatctc ccttatcgtt ctggaaaggt gttctcccct
     1081 ggaaaccctt tcctgaaagc gatcaaggtc gcccccctgg gacctgacca tctcctcgaa
     1141 cttgcggaaa ccctttccct cctcaaggga tctctcggcg aggctcaggg cttcatccag
     1201 cgatatggag ggtctcccga gatggaccat tgatgccgtc agtctcaggg agagctccct
     1261 caggtcgtcg ggccctctcc cgagcaggag gtcgaccgcc tcggcgactt ccgcggcatt
     1321 cccgacccac atccccaggg gttgggacat gtccgttatc agcgccatgg agccccttcc
     1381 cattgaggca gaaagatcca cgaggctctc tgccagttcg cgggcagaga gctgatcttc
     1441 catgaaggcg ccgctgccgc acttcacatc aaagacgaac gccctcgatc ccccggccat
     1501 cttcttgctg acaatgctgc tggtgatgag tggtatggac gggatagtac ctgtaacatc
     1561 cctcaattcg tagaaaaggg cttcagccgg tgccaggtcc ccggagtggc cgcttatggc
     1621 acaccctgta cttcttacct ggtcgatgaa tcgtcccatt tcaaggtggg ccgaaaaacc
     1681 cgggatggat tcaagcttgt cgacagtgcc accagtgaac cccaaccccc tgccgctgag
     1741 cttggctaca ggcactccgc aggccgcggc caggggcacc accacaaggc tggttttgtc
     1801 gcccacaccg ccggtgctgt gcttgtccac ggggttgata tccggaggga aattgatcac
     1861 tcttccggat ctcgagagag cttcggtgaa ggtccgggtc tccatgccgg acattcccct
     1921 gaaaaagacc gccatcaacc atgcggctac ctggtattcc gggatctctc ccttgtgggc
     1981 caggttgacg aaatcacgga tctctgaagt cgtcgcctct ttcccgtctc gttttgactc
     2041 gatgaaggct acagggttca tcggctcctg gcacccagcc tgcctatcat ggcct
//
LOCUS       DAOPKQ010000237         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_63503_length_2058_cov_3.977034, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000237 DAOPKQ010000000
VERSION     DAOPKQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63503_length_2058_cov_3.977034"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PK146_07145"
     CDS             complement(<1..116)
                     /locus_tag="PK146_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein"
                     /protein_id="HPE92050.1"
                     /translation="MTRTVWYPGHMARGMRKLRELSEKIDLFIEVRDARAPLL"
     gene            complement(113..631)
                     /gene="lepB"
                     /locus_tag="PK146_07150"
     CDS             complement(113..631)
                     /gene="lepB"
                     /locus_tag="PK146_07150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPE92051.1"
                     /translation="MAVKSWWREMIETVLWALVLALVLRALVIQAFWIPSGSMIPALL
                     PRDRVLVAKFWYSFAEPKRGQIIVFKYPVDPKRDFVKRLVALPGETVEIIDGEVIIDG
                     RSLEEPYVKNHDTLSFGPVTVPEGHYLMLGDNRPNSQDGRFWGFVPEANIKGPAFLRY
                     WPLSRIGGLYEE"
     gene            complement(695..1237)
                     /locus_tag="PK146_07155"
     CDS             complement(695..1237)
                     /locus_tag="PK146_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92052.1"
                     /translation="MKGSAEPGPEKGRGAPGGLPVFSPRNRLKGYSIAELLVVLLLVS
                     MFAAMLYPVVGGLLASRTGTPESARMEAKRVERWLNRILQTACIRQRPFDIVHQAGYK
                     DNILVHWYNPSEYERYRTGGRCTVYFKTTSCSRCYSPRWHTMSPAFTMSFHVPETEKR
                     KVAEIVVSVYCRVLLTEFFP"
     gene            complement(1221..1775)
                     /locus_tag="PK146_07160"
     CDS             complement(1221..1775)
                     /locus_tag="PK146_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPE92053.1"
                     /translation="MQYVIVGIGGQGILFSSRVLGHIALSGGERVIGSEVHGMAQRGG
                     SVISHFKVGDYNSPLVKAGEADVLLAFDQNEAVRNMHFLKHGGHVVVNTHDSSFENNA
                     SIRSWLADHDAKLHTIMGYEILKKEMGGRFLFLNVLILGAMCGAGVGGVSIEEVRDAV
                     SSLAPARFVEDNVKAVNLGYEGIR"
     gene            complement(1778..>2058)
                     /locus_tag="PK146_07165"
     CDS             complement(1778..>2058)
                     /locus_tag="PK146_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPE92054.1"
                     /translation="WEHARTNREPAVVIFRHPCMLFKEKQAVIPVKVDPEKCIGCKFC
                     INYFNCPGLVFDEESKKAYIDERFCVKCGVCVNVCPHGAILPTEGEEA"
BASE COUNT          436 a          640 c          532 g          450 t
ORIGIN      
        1 agaagggggg ccctcgcgtc gcggacctcg atgaaaaggt cgatcttttc cgaaagttcg
       61 cggagttttc tcatcccccg ggccatgtgg cccgggtacc agacggtgcg ggtcattcct
      121 cgtaaaggcc gcctatcctc gacaggggcc agtatcgcag aaaggccgga cccttgatgt
      181 tggcctccgg gacgaaaccc cagaacctcc cgtcctgtga atttggcctg ttgtccccca
      241 gcataaggta atgcccttcc gggacggtta cggggccaaa actcaaagtg tcgtggttct
      301 tgacgtaagg ttcctccagc gatctgccat ctatgatcac ttccccgtcg attatctcga
      361 cggtctcccc cgggagggcc acaagcctct tcacgaaatc cctcttcggg tcaacgggat
      421 acttgaagac gatgatctgt ccccgtttcg gttcggcgaa ggagtaccag aacttggcca
      481 ccagcaccct gtccctgggc aaaagggccg gtatcatcga gccgctcggt atccagaagg
      541 cctgaataac cagcgccctc agcacgaggg ccagtaccag cgcccaaaga acagtttcta
      601 tcatctctct ccaccaggat ttgacggcca tgatccattc ctccatatct cagtcttctg
      661 acgttcccgg ttatacccca aagggcaaaa gccttcaagg gaaaaactcc gtaagcagca
      721 cacgacagta aaccgacaca actatctcgg caacctttct tttctctgtt tccgggacgt
      781 ggaaggacat cgtaaaggcc gggctcatgg tgtgccacct gggtgaatag cacctcgagc
      841 aggaggtggt cttgaaatag accgtgcacc ttcccccggt cctgtatctt tcatactccg
      901 acgggttgta ccagtgtacc agtatgttgt ccttataccc cgcctggtgg acgatatcga
      961 aaggtctctg cctgatgcag gcagtctgaa gtatcctgtt aagccacctt tctacccttt
     1021 tggcctccat cctcgctgac tccggggtcc ccgtcctgga agccagcaac ccccccacaa
     1081 cggggtacaa catggcggca aacatggaga ccagcaggag gacgacgaga agctccgcta
     1141 ttgaataccc ttttaacctg tttcggggcg aaaaaacggg gagccctccc ggagctcccc
     1201 tgcctttttc cggaccgggt tcagcggatc ccttcatacc ccagattcac ggccttgacg
     1261 ttatcttcga cgaatctggc gggggcgaga ctgctcaccg cgtccctgac ctcctctatg
     1321 gaaactccgc cgacaccggc gccgcacatg gcacccagga tcagcacgtt aaggaaaagg
     1381 aaacgccccc ccatctcttt cttgagtatc tcgtaaccca ttatcgtgtg gagtttcgcg
     1441 tcgtgatctg ccagccacga tcttatcgat gcattgttct cgaatgagga atcgtgggtg
     1501 ttgaccacca cgtgtccgcc gtgtttcagg aaatgcatgt tccggacggc ctcgttctgg
     1561 tcgaaagcca gcaaaacatc ggcctctccc gcctttacca gggggctgtt gtagtccccg
     1621 accttgaaat ggctgatgac ggaaccgccc ctctgggcca ttccatgaac ctcgctgccg
     1681 atgaccctct caccgccgga gagggcgata tgcccgagga cacggctcga gaaaagaata
     1741 ccctggcctc caatgccaac tatcacgtac tgcatggcta tgcctcctcc ccttcggtcg
     1801 gcaggatggc cccgtggggg cagacattga cgcagactcc gcacttcacg cagaagcgct
     1861 cgtcgatata cgccttcttg ctctcctcgt cgaagacgag tccggggcag ttgaaatagt
     1921 tgatgcagaa cttgcagcct atgcactttt cgggatcgac cttgacggga ataacggcct
     1981 gcttctcctt aaaaagcatg cagggatgcc tgaaaatgac caccgccggt tcccggttgg
     2041 tcctggcgtg ctcccagg
//
LOCUS       DAOPKQ010000238         2050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_63889_length_2050_cov_4.705263, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000238 DAOPKQ010000000
VERSION     DAOPKQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63889_length_2050_cov_4.705263"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..616
                     /locus_tag="PK146_07170"
     CDS             284..616
                     /locus_tag="PK146_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HPE92055.1"
                     /translation="MPKNIAEEITEAEARARQILQDARTEGARLLAQAKAEAEDLAKG
                     SRQSYHRTLREKVLKMEEEADKVSEKTIEKGRDDSKELVKSHKAKVDDVANWVAEEVM
                     ARYGSNKS"
     gene            597..>2050
                     /locus_tag="PK146_07175"
     CDS             597..>2050
                     /locus_tag="PK146_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPE92056.1"
                     /translation="MAVTRVKKVRILSHQDILENVLAALQNLGCCEMISSDEQGVPGE
                     EYAAFPPDRARSERSSDLEASIGDCRSLLRFLEPLYEEEGGMLERLLGPKPPVTLSEL
                     ASLRSSTDLSDLATKARGLERRISEKRSETTMVTNHIKLLEPLKDLDLPLWIVSRGTE
                     KVTGVLGTIPAARKEELTDSFSSLVKGQGEVFLAAHGDKDVEVMAVLVFPRSIEREVL
                     ETASGAGFSKVDCPQGLEDQPDQEIVRLVSLREDLARDISSAEEEASRMALDVVPVAR
                     KINDFLSVLSARRDAGRSGVSTEKVSALRFWIPADKLDDLEKILEPYATLIDIHSEDP
                     APEDNPPIILDNPKWAVPFSPLTKLYGMPSYTGIDPTVLMAPFFFLFFGMCLGDGGYG
                     LVMGAFFLFALVRYRLSGDTRNFFILLILGSVSTVLVGALTGSWLGDMIDVFGIFAPI
                     RSMKDRLVLIKPMNDPITFLAISLALGVIQILFG"
BASE COUNT          483 a          528 c          581 g          458 t
ORIGIN      
        1 agcatcggct tcccaagccg agggtcgcgg gttcgaatcc cgttgtccgc tccatttttt
       61 ttgtgctatc cttgaccagg taacccttca tttcctttgg acccccttcc agtaaacgat
      121 accaggggag tcttgtcctc tcagatcatt gatatcggca agggccctcg gggagcgccg
      181 gggcgtatcg agttgacacg acaagggtgg ggcaattaga atcttgtagg tttgttaaaa
      241 aagtatcttt atacagtatt atgcatgctg gggggtaatt catgtgccga agaatatcgc
      301 ggaagaaatt accgaagcgg aggcaagggc cagacagata cttcaggacg ccaggactga
      361 aggagcaagg ttattagcgc aggccaaggc ggaagcggaa gatctggcca agggatcaag
      421 gcagagttat catcggaccc tcagggaaaa ggttctcaaa atggaggaag aagcagataa
      481 ggtttcagag aagacgatcg aaaagggaag agatgattct aaagaactgg tgaagagcca
      541 taaagccaaa gttgacgatg tcgctaactg ggtcgctgaa gaggtgatgg cacgatatgg
      601 cagtaacaag agttaagaag gtcaggatcc tctcccacca ggatatactg gagaatgtcc
      661 ttgcggcgct tcagaacctt gggtgctgcg aaatgatctc ctccgatgaa cagggcgttc
      721 ccggagagga gtacgcagct tttccccctg accgggcgag atccgaaagg tcatcggacc
      781 tcgaggccag tatcggtgac tgccgatccc tcctcagatt ccttgagccc ctctacgaag
      841 aagagggcgg aatgctcgaa aggctccttg gtcccaaacc ccctgttaca ctgtccgaac
      901 tcgcctcgct cagatccagt accgatctct ccgatcttgc aacgaaagcg cgaggcctgg
      961 aacgccgtat ctccgagaag agatcagaga cgacaatggt cacgaatcac attaagctcc
     1021 tggaacctct gaaagatctg gacctccccc tttggatcgt atcaaggggt accgagaagg
     1081 tgacgggagt tctgggcact atccctgcgg cacggaagga agagctgacc gacagctttt
     1141 ccagcctggt gaaaggacag ggagaggtct tcttggccgc acacggggat aaggatgttg
     1201 aggtcatggc cgtactggtc tttccccgtt cgatagaaag ggaggtcctg gaaacagctt
     1261 ccggcgcggg tttctccaag gtggactgtc cccagggact tgaagatcag cccgaccagg
     1321 agatcgtgag actcgtgtct ctcagggagg accttgccag ggatatctcc tcggcggaag
     1381 aggaggcctc ccgtatggca ctggacgttg ttccagtggc aaggaagatc aacgactttc
     1441 tttctgtgct ctcggcgagg agagatgccg gccgttcggg agtatcaacg gagaaggtgt
     1501 ctgccctcag gttctggata ccagcagata agctggatga cctggagaaa atcctggaac
     1561 cctatgcgac cctgatcgat atccattccg aggaccctgc acccgaggac aaccccccga
     1621 taatcctgga caaccccaaa tgggccgttc ctttttcccc tctcacaaag ctttacggga
     1681 tgccctcata caccggtatc gacccgacgg tgctgatggc cccgttcttc ttcctgttct
     1741 tcggcatgtg cctgggagac ggagggtacg gtcttgtcat gggggctttt ttcctatttg
     1801 ccctggtacg gtacaggctt tcaggtgaca cgaggaactt tttcatcctt ctgatcctgg
     1861 gcagcgtctc gacagtcctt gttggtgcac tgaccggaag ctggctaggg gacatgatcg
     1921 acgtttttgg gatctttgcc cccataaggt cgatgaagga cagactggtc cttatcaaac
     1981 ccatgaacga ccccattacc tttctggcga tctcccttgc ccttggcgtt atccagatac
     2041 tcttcggcat
//
LOCUS       DAOPKQ010000239         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_64668_length_2032_cov_4.455235, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000239 DAOPKQ010000000
VERSION     DAOPKQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64668_length_2032_cov_4.455235"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PK146_07180"
     CDS             complement(<1..1247)
                     /locus_tag="PK146_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPE92057.1"
                     /translation="MRMGFDMDQEKTTQTPLVQEIQEEELQSIVEKYDIESRYRRPAG
                     FSKIVVSVWLIAMSLFHLYTAGIGLLPTSIHRAVHLTFAIVAVFLLYPGRKGQDKTRI
                     PWYDWILAATAGLGTGYIVFFFNEIARRGAQVQPHEVWLGILTIVMVLEAGRRVAGKV
                     LPSLSILFLLYCYFGRYMPFMFMHRGYSINRIIQHMYLTQEGIFGVALGVSSTFVFMF
                     ILFGAFLGKSGGARFFNELALAIAGHSPGGPAKVAIVASGLLGTINGSSVANVATTGA
                     FTIPLMKKVGYKPEFAGAVEACASTGGQFMPPIMGAGAFIMSEFLGISYLRIAAAAII
                     PAVLYYSALFIAVHLRARHRNLEGLSKEVLPALKDVLVKDGHLVVPLVVIIGMLLNRF
                     TPLAAAFWGIISVVAVSLLRKHTR"
     gene            complement(1314..>2032)
                     /locus_tag="PK146_07185"
     CDS             complement(1314..>2032)
                     /locus_tag="PK146_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPE92058.1"
                     /translation="SGEAEVAFMDGLYYYAYLGMDKFEGNPQDHIRALVPLYPEPTQL
                     MVAKGSGIKTLQDFKGKRVSIGAVASGTEVTARQLLRLAGIDPDKDIQAENLGVGDTA
                     TAFSDNRIDAAVMVGSLGMAGVIEATTLGVVEFIDVPDDIRDKVIAETPYWVPFNIPA
                     NFYSNQPNEVKTYASWNILAIHKDVDGELAYQMTKALFEHKDDLLAVRATMQTMTPEN
                     IEYILIPMHYGARRYYDEVL"
BASE COUNT          415 a          554 c          613 g          450 t
ORIGIN      
        1 cgggtgtgtt tgcggagcag gcttacggca actacgctga ttatccccca gaacgccgct
       61 gccagaggtg tgaacctgtt cagtagcatg ccgataatta ctaccagagg gacaacaaga
      121 tggccgtcct ttacaagaac atccttaagg gcagggagaa cttctttcga aagcccttcc
      181 aggttcctgt gcctggccct gaggtgtacg gctatgaaaa gggcagagta gtagagtacc
      241 gcaggaataa tagcggcagc cgctatcctg aggtaggaga tccccaggaa ttcgctcatt
      301 atgaaggccc cggcccccat gatgggaggc atgaactggc ctcctgtgga tgcgcaggcc
      361 tcgacggctc ctgcgaactc cggcttgtag cccacctttt tcatgagcgg tatagtgaac
      421 gctcccgttg tggccacgtt ggcaacggag gagccgttga tagtccccag aagtccggag
      481 gccacgatgg cgacctttgc aggccctccg ggtgagtgcc ccgcaattgc cagtgccagt
      541 tcattgaaga atctggcccc tccgctcttg cccagaaagg cgccgaagag gatgaacatg
      601 aaaacgaacg tgctcgatac cccgagggca acaccgaaga tcccctcctg ggtcaggtac
      661 atgtgctgga ttatccggtt gatggagtag ccccggtgca tgaacatgaa gggcatgtat
      721 cgtccgaagt agcagtagag gaggaagagg atgctgagag atgggaggac tttgcccgca
      781 acccttcttc ctgcctcaag gaccatcaca atggtcagga tgccgagcca gacctcgtgg
      841 ggctggacct gggctcctct ccgggcgatc tcgttgaaga agaagactat gtatcctgtg
      901 cctaaacctg ccgttgcagc caggatccag tcataccagg gtatcctggt cttgtcctgc
      961 cccttgcgcc cggggtagag caaaaaaacg gcgacgattg cgaaggtaag gtggacggca
     1021 cggtggatcg aagtcggcag aaggcctatc cccgcggtgt acaggtgaaa gagggacatg
     1081 gctatgagcc agacggagac gacgatcttg gaaaagcctg cgggccttct gtatcggctc
     1141 tctatgtcgt acttctctac gatactctgg agttcttcct cctggatctc ctgtaccagt
     1201 ggtgtctggg tggttttttc ctggtccatg tcaaacccca ttctcataag ggcccgggcg
     1261 gcagattccc cggcccgggc cctgttgtca gatatcttta atgagtagtg ctcctatagt
     1321 acttcgtcgt agtaccttct ggcgccgtag tgcatgggaa taaggatgta ctcgatgttc
     1381 tccggggtca tggtctgcat ggtggcacga acggcgagga ggtcatcctt gtgctcgaac
     1441 agcgccttgg tcatctggta ggccagttcg ccgtccacat ccttgtggat ggcaaggatg
     1501 ttccagcttg cgtaagtctt gacttcattg ggctggttgg agtagaagtt cgccggaatg
     1561 ttaaagggta cccagtaggg ggtctcggcg atgaccttgt cgcggatgtc gtcggggaca
     1621 tcgatgaact cgacgactcc cagggtcgtg gcctcgataa cgcccgccat tcccagggaa
     1681 cctaccatca ccgcagcgtc gatcctgttg tcgctgaaag cggtggctgt gtcgccaaca
     1741 ccgaggttct cggcctggat atccttgtca gggtcgatcc ccgcaagcct gagaagctgc
     1801 cgggcggtga cctctgtgcc gctggcaacg gccccgatgg agactctctt gcccttgaag
     1861 tcctggaggg tcttgatacc gctccccttg gcgaccatga gctgggtcgg ctccgggtag
     1921 agggggacaa gggcccttat atggtcctgg gggttaccct cgaatttgtc cattcccagg
     1981 taggcgtaat agtaaagccc gtccatgaag gccacttcgg cctctccgct gc
//
LOCUS       DAOPKQ010000240         2030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_64763_length_2030_cov_6.654177, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000240 DAOPKQ010000000
VERSION     DAOPKQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64763_length_2030_cov_6.654177"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PK146_07190"
     CDS             complement(<1..658)
                     /locus_tag="PK146_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPE92059.1"
                     /translation="MELTSSVVLDWITLYALKLVAALLILVVGRIVAKGIRSLIRKAL
                     SKWNVDVTLVKFIATLSYFSIMAFVIVAALAQIGVQTASFVAIVGAAAFAVGLALQGS
                     LANFASGVLIILFKPFKAGDYVEGGGVAGTVEEVSIFTTVLRSPDNRVIIVPNGKIMA
                     DNIVNYSAREIRRVDLVAGVSYSDDIDKVRKVLEIILDEDERVLKDPAPTIGVLELAD
                     S"
     gene            complement(770..1168)
                     /locus_tag="PK146_07195"
     CDS             complement(770..1168)
                     /locus_tag="PK146_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPE92060.1"
                     /translation="METRMVTVTSEEMAKGIEEAGRIALYGIFFDTDKTDIKPESVPT
                     LEEIAKLMKALPELRVLVVGHTDNTGDREYNMGLSSRRAESVVKFLQDKFQIPATRMI
                     PAGLGMLAPVASNRTEGGRANNRRVELLEF"
     gene            1299..1736
                     /locus_tag="PK146_07200"
     CDS             1299..1736
                     /locus_tag="PK146_07200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPE92061.1"
                     /translation="MKVIEAGVFVQASSEKVWRVLSDLGKYPEWNPFILMISGELEEG
                     ARLSVKIGLPEGRPMTFSPVVRVAKPGVELRWLGHLWFPGLFDGEHSFRIDEAGKGHV
                     HLMQSEHFRGILVPFLPDSFFERTRQGFEEMNRALKERVESSL"
     gene            complement(1733..>2030)
                     /locus_tag="PK146_07205"
     CDS             complement(1733..>2030)
                     /locus_tag="PK146_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPE92062.1"
                     /translation="AFLVGISWSLYLIAFPLVIPLAQALGADLVLAVAATVAAGTLGQ
                     HCCFYSDGTILVSASTGCDNIRHALTQLPFAMIAAGLACVGFLVAGFLSTQGFH"
BASE COUNT          448 a          553 c          555 g          474 t
ORIGIN      
        1 tgctgtcagc cagttccagc actccgatgg tcggggcagg gtcctttagg acacgttcat
       61 cctcatccag tatgatctcc agcaccttcc tgaccttgtc gatatcgtca gaatagctca
      121 ctcctgccac aagatcgaca cgacggatct ctctggcgga atagttgacg atattgtcgg
      181 ccatgatctt cccgttgggc acaatgatca ccctgttgtc gggcgatctc aggacggtgg
      241 tgaagatgct gacctcctca acggtccctg ctacaccacc gccctcaaca tagtcccctg
      301 ctttgaaggg tttgaagagg attataagta cccctgacgc aaagtttgcc agcgaaccct
      361 gaagggccag acctacagcg aaagcggcag ctcccactat ggctacgaag gatgcagtct
      421 ggacgcctat ctgcgcaagg gcggcaacga taacgaaggc cattatggag aagtatgaga
      481 gagtggcaat gaacttcacc agcgttacgt ccacattcca ttttgaaagg gccttcctga
      541 tcaatgaacg tatccccttg gcgacaatgc ggcccacaac aagtatcagt agcgcggcta
      601 ccagtttcag cgcgtacaga gtgatccagt cgagcacgac cgatgatgtt agttccatga
      661 gagtacctcc gttcccttga tttggatcaa tgactatacc ttacttgaaa atatcaccta
      721 tgtccctgtg agggttatcg aattcaaaaa ctgcggggcg gagaaggtgc tagaactcca
      781 acagttctac cctcctgtta ttggctcttc ccccctctgt cctgttggaa gcgacgggcg
      841 ccagcattcc cagacctgcg gggatcatcc tcgttgcagg gatctggaac ttgtcctgga
      901 ggaacttcac aaccgattca gctctcctgc tggaaagccc catgttgtac tccctgtcgc
      961 cggtgttgtc ggtgtgcccc accaccagga ccctcagctc cggcaatgcc ttcatgagtt
     1021 tggcgatctc ctccagagtc ggcactgact ctggttttat gtctgtcttg tcggtatcga
     1081 agaatatccc gtagagtgct atccttcctg cttcctcgat ccctttcgcc atctcctctg
     1141 aggtgactgt caccatcctg gtctccatta taataggatg gcgatcagcg aacggggggc
     1201 aacattctta ggaaagtttt tcgttcctga cctgatccag tcaccggggt ctccatttgt
     1261 agctaccgga tgttcgaacc actcctggag gtgatgtctt gaaagtaatt gaagcagggg
     1321 tcttcgtaca ggccagctcc gaaaaggttt ggagagtact ctcagatctg gggaagtacc
     1381 ccgagtggaa tccgttcatt ctgatgatat ccggtgagct ggaagagggg gccaggcttt
     1441 cggtgaagat cggactgccg gaggggagac ccatgacctt ttctcccgtg gtcagggtcg
     1501 caaaacctgg agtggaactg cgatggcttg gtcatctgtg gttcccgggt ctttttgacg
     1561 gagaacactc cttcaggata gatgaggccg ggaaggggca tgtccatctg atgcagagcg
     1621 agcatttccg cgggatcctg gtcccattcc ttcccgattc tttctttgaa aggaccaggc
     1681 aaggcttcga ggagatgaac cgtgccctga aggagagggt cgagagttcc ctctaatgaa
     1741 agccctgtgt cgacaggaag cctgcgacca ggaatccgac acaggcgagg cctgctgcta
     1801 tcatcgcgaa aggtagctgg gtcagggcgt ggcggatgtt gtcacacccc gttgaggctg
     1861 acacaaggat cgtgccgtca gagtagaaac agcagtgttg gcccagggtc cctgcggcaa
     1921 cggtcgctgc cacggccagg accagatccg ctcccagagc ctgtgcgaga gggatcacca
     1981 gcgggaatgc gattaggtac agactccagc ttattcccac aaggaatgcg
//
LOCUS       DAOPKQ010000241         2030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_64769_length_2030_cov_4.692152, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000241 DAOPKQ010000000
VERSION     DAOPKQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64769_length_2030_cov_4.692152"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..878
                     /locus_tag="PK146_07210"
     CDS             51..878
                     /locus_tag="PK146_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPE92063.1"
                     /translation="MFPLPFHLWNSKGNIRVQLGPHAPYTVSLGVLKDIVGSAADLGS
                     GVHFHFLEAEWEAAFIREKFGLSPLAYLEEAGLLETRATILAHCVWFPEGEVPELAGT
                     SVTVAHNPTSNMKLGSGHAPLQCFLEAGVPVSLGTDGAASNNRLDIWGEMRLCALIHK
                     GIAKDPTAIAARDIFRMATLSGASAAGFENSGLIREGWQADLVMVDLDRPHYIGWDPD
                     NLIGFLVYAGSSSDILGTMAAGRWLFREGEFPGQDRGEVMGEISRCREDLLDRAVRR"
     gene            920..>2030
                     /gene="alaS"
                     /locus_tag="PK146_07215"
     CDS             920..>2030
                     /gene="alaS"
                     /locus_tag="PK146_07215"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPE92064.1"
                     /translation="MRWRSGKELRELFLSYFERKDHRRFPSFSLVPDDPSILFTIAGM
                     VPFKPYFLGMRVPSFNRATTSQKCVRTNDIENVGRTARHHTLFEMLGNFSFGDYFKSD
                     AAAFAWEFLTGEVGLDPDRLCPTIFREDDEAFSIWNGEIGVPADRIYRMGEEDNFWSV
                     GPVGPCGPCSELIYDQGPSFSCGRPDCGVGCDCDRFLEVWNLVFMQYNRLEDGSLEPL
                     PKKNIDTGMGLERLSSVVQQVAGDFQTDLFKPLVEKSCEICGIRYGEDPMGDMAARVI
                     SDHFRATAFMIADGVLPSNEGRGYVLRRILRRAVRFGRLFGVERPFLMEMYPTLLEVM
                     SDPYIELSEQRPLVEQVIEMEEDRFAEALHRVLKRG"
BASE COUNT          411 a          569 c          636 g          414 t
ORIGIN      
        1 ggacaccaag atagacaggg tagggggcgt agggtcccag ctggaccctg atgttcccct
       61 tgccgttcca tctttggaac agcaagggga acatcagggt ccagctggga ccccacgccc
      121 cttacactgt ctctctgggt gtcctgaagg atatcgtggg ttctgcggca gatcttggtt
      181 ccggtgttca ctttcatttt ctcgaggccg agtgggaggc agccttcata agggagaagt
      241 tcggtctctc ccccctggcc tacctggagg aggcgggact tctggagacc agggccacta
      301 tcctggccca ctgtgtatgg ttccccgagg gagaggttcc ggagctggca ggcacctccg
      361 taacggtggc ccacaatccg acgagcaaca tgaaactggg gagcggtcat gcgccgctac
      421 agtgcttcct ggaggcgggc gtgccagtgt cgctcggtac cgacggggcc gccagcaaca
      481 acaggctcga catctgggga gagatgaggc tgtgcgccct gatacacaag ggcatcgcca
      541 aggaccctac ggccatcgca gccagggata tcttcagaat ggccaccctg tcaggggcgt
      601 cggccgctgg tttcgagaat tctggcctta tcagggaggg ctggcaggct gacctggtga
      661 tggtcgatct tgaccgcccg cactacattg gctgggaccc cgataatctc atcggcttcc
      721 ttgtctacgc cggttccagt tccgatatac tggggacgat ggccgccggc aggtggctct
      781 tccgcgaggg ggagttcccc ggtcaggaca ggggagaggt catgggcgag atctccaggt
      841 gcagggagga cctcctggac cgtgccgtcc gccggtagtg atataatcgc cagaaaaaac
      901 agaaggagac atccggaaga tgcgatggag aagcggaaag gaactcaggg agctgttctt
      961 gtcctacttc gagagaaagg atcacaggag attccccagc ttttcgctgg tgcctgatga
     1021 tccgtccatt cttttcacaa tagctggcat ggtccccttc aaaccctatt tcctggggat
     1081 gagggtaccc tccttcaaca gggccaccac ctcccagaag tgcgtccgta ccaacgatat
     1141 cgagaatgtc ggcaggaccg ccaggcatca tacccttttc gagatgctgg gcaacttcag
     1201 cttcggcgac tatttcaaaa gcgatgcggc agcctttgca tgggagttcc ttaccggcga
     1261 ggtggggctg gaccccgaca ggctctgccc caccatcttc cgggaggacg atgaggcctt
     1321 ctccatatgg aacggagaga tcggcgttcc ggcggaccgc atctaccgca tgggcgagga
     1381 ggacaacttc tggtctgtag ggcccgtagg cccctgtgga ccctgttcgg agctcatcta
     1441 cgaccagggc ccctccttct cgtgcggaag gcccgactgc ggtgtgggtt gcgattgtga
     1501 ccgcttcctt gaagtgtgga acctggtctt catgcagtac aaccgcctcg aggacggatc
     1561 cctggagcca ctgccgaaaa agaatattga cacgggaatg ggcctggagc gcctctcttc
     1621 ggtggttcag caggtggccg gggatttcca gacggacctc ttcaagcccc ttgtcgaaaa
     1681 gtcctgcgag atatgcggta taagatacgg tgaagacccg atgggagaca tggccgcaag
     1741 ggtcatctct gaccatttcc gggccacggc tttcatgata gccgacgggg tactcccatc
     1801 caacgagggg cggggctacg ttctcaggag gatcctgcga agggccgtac gtttcggaag
     1861 actcttcggc gtcgaaagac cattcctgat ggagatgtac ccgacactgc tggaggttat
     1921 gagcgacccc tacatcgagc tttcggagca acgccccctt gtggaacagg tcatcgaaat
     1981 ggaggaggat cggttcgcgg aggcattaca tcgtgtgctc aaacggggcg
//
LOCUS       DAOPKQ010000242         2008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_65805_length_2008_cov_4.202765, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000242 DAOPKQ010000000
VERSION     DAOPKQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65805_length_2008_cov_4.202765"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK146_07220"
     CDS             <1..531
                     /locus_tag="PK146_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPE92065.1"
                     /translation="LPKDCSTGSVRNLVEALAVSGFKDRVLVDLSIARELDYYSGPVF
                     EVFCGSHGRPVGGGGRYDGLLSSFGVLGQAIGFSLNLDLLLYRGTSSVSKSLAVMTWS
                     GDLRPETAMKLAAELAACGVTVEMSWIDQRERSIELARRRGYRWWTDLSDGSIYDLEN
                     NSPVDRAYWLPDGGRT"
     gene            528..1178
                     /gene="hisG"
                     /locus_tag="PK146_07225"
     CDS             528..1178
                     /gene="hisG"
                     /locus_tag="PK146_07225"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPE92066.1"
                     /translation="MISIAIPTGRMMKECATILAQLGLPCSRLENPGRALVISERDRR
                     YLLAKPMDVPAYVHYGAADMALAGSDVLMERKVPLVELADTGAGRCRLVIAAPDPLVR
                     KFASPGNHLNGLKVATKYPNIATSFFQSLGLKLEVVHMHGSVELAPILGISDCILDIV
                     QSGETLKANGLKVIQEVAHVSMRLVASRRFTQTGWSMISGIMGELKPWKGRNYNAE"
     gene            1168..1737
                     /locus_tag="PK146_07230"
     CDS             1168..1737
                     /locus_tag="PK146_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HPE92067.1"
                     /translation="MPNEFERTTAETSVRVSFETEPGTPSIKTPCGFFSHMLELLGAN
                     MGWSLSIEASGREGEDLHHIVEDVGIALGRALFSWYPSSPRARYGWCALPMDGSLALI
                     SVDLSGRGGCYFRGSFPSERCGDLDLELVPEFFSALARESRTTIHARLLEADNSHHAA
                     EALFKATGRAFAQAFIPSQTSSSTKGVWP"
     gene            1734..>2008
                     /locus_tag="PK146_07235"
     CDS             1734..>2008
                     /locus_tag="PK146_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPE92068.1"
                     /translation="MIGLIDYGAGNIGNVERALKHLGRDSAALDSPDAPCSGVLEALL
                     LPGVGSFPSAMASLFSRGWPSFISEWVRMERPLIGICLGMQILCEGST"
BASE COUNT          405 a          556 c          616 g          431 t
ORIGIN      
        1 cttccgaagg actgttccac cggaagcgtc aggaaccttg ttgaagccct tgctgtctcg
       61 ggcttcaagg acagggtcct ggttgatctc agcatagcca gggaactgga ctactacagc
      121 ggacctgtct tcgaggtctt ctgcgggtct cacggccgtc ctgtgggggg tggggggcgc
      181 tacgacgggc tcctttcatc atttggcgtc ctgggccagg ccatcggttt ctcgctgaac
      241 ctggacctcc ttctctaccg gggcacctcg tccgtttcca aatctctagc ggtcatgacc
      301 tggtccggag atcttcggcc ggagacagca atgaagctgg ccgcggagtt ggccgcttgc
      361 ggtgtaaccg tagagatgag ctggatagac cagagggaaa ggtccatcga actggccagg
      421 aggcgaggct accgctggtg gaccgacctg tccgatggat ccatctacga cctggagaat
      481 aacagccctg ttgaccgggc ctactggttg cctgacggag gaaggacatg atcagcattg
      541 ccatcccgac gggaagaatg atgaaggagt gcgcaacgat actggctcaa ctcggactcc
      601 cctgctctcg ccttgaaaac cctggccgag ccctggtcat ctccgagagg gatcgacgtt
      661 atctgttggc caaaccaatg gacgtacctg cctacgtgca ctacggggcc gcagatatgg
      721 ccctggccgg gagcgatgtc ctcatggaac gcaaggtccc tctggtggag ctagccgata
      781 caggggctgg gaggtgcagg ctggtcattg ccgctccaga tcctctggtc agaaaatttg
      841 cctctccagg gaatcatctg aacggcctca aggttgccac caagtacccc aacatagcaa
      901 cgtccttctt ccaatccctt gggctcaaac tggaggtcgt ccatatgcac ggatcagtgg
      961 aactggcacc tattctcggg atcagcgact gcatacttga tatcgtccag tcgggggaga
     1021 cccttaaggc gaacggactg aaggtcatac aggaggtggc ccatgtctcg atgagactgg
     1081 tggccagcag gcggttcaca cagacagggt ggagcatgat atccgggatc atgggtgaac
     1141 tgaaaccatg gaaaggaagg aactacaatg ccgaatgagt tcgagagaac gactgcagag
     1201 acttcggtca gggtctcatt cgagaccgaa ccgggtacgc catctatcaa aacaccctgc
     1261 gggttctttt cccacatgct ggagcttttg ggggctaata tggggtggag cctgtcgatc
     1321 gaggcgtcgg gaagagaggg agaagacctt catcacatcg ttgaggatgt gggtattgcc
     1381 ctgggaaggg ctctcttctc atggtaccct tcttctcccc gagcccgtta cggctggtgc
     1441 gctctcccaa tggacggttc gctggcgctg atctcggtgg atttgagcgg aagagggggt
     1501 tgttacttcc ggggctcctt cccatccgaa agatgcggcg atctggacct tgaactcgtg
     1561 ccggagttct tctcggccct ggcaagggag tcgagaacga cgatccatgc caggctcctg
     1621 gaggctgaca actctcacca tgcggcagag gctctcttca aggccaccgg acgtgccttt
     1681 gcacaggctt ttatcccttc ccagacctcg tcttcaacga agggggtctg gccatgatag
     1741 gcctgatcga ctacggagcc gggaacatag gtaatgttga gagggctctg aagcacctcg
     1801 ggagggacag tgcggccctg gattctccgg atgccccatg ttcgggggtt ctggaggccc
     1861 tcctgcttcc cggagtgggg tctttcccgt cggcaatggc atccctgttc tcccgcggct
     1921 ggccttcctt catatccgaa tgggtaagga tggaacggcc cctgatcggc atctgcctcg
     1981 gaatgcagat cctgtgcgaa ggaagcac
//
LOCUS       DAOPKQ010000243         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_66022_length_2004_cov_3.880965, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000243 DAOPKQ010000000
VERSION     DAOPKQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66022_length_2004_cov_3.880965"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="PK146_07240"
     CDS             complement(<1..1687)
                     /locus_tag="PK146_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPE92069.1"
                     /translation="MVPNKWIPYKDFDGRPNYEQRNWLIGSGSIGGKAKGLSFAFDHL
                     QGSELMEDVKLPSMTYVITTEPFHDFLNDNVMDWIHDEPDLEKIILAFSQGRLRESFL
                     NDLDKVLQTLYDTPLAIRSSSLLEDSHLLSFAGKYFTTFAANVFDMEWRRRELADGIK
                     TVWASLYNQAARAYRTKHGFADRDEAMAVLVQPLIGHDHDGLYYPELAGTIFSRVYRR
                     PSPRIDKEQGVIRFCFGLGTRTVDRSLARVVYLSHPNMRPEGNLAREVVRYSQSSFDY
                     VDLEFGAFVSGDLRSLFPFIMEHHKSATAFVELFGDNILYWAGSRPMIPTTPFFTFGD
                     LPTRYPHFLETARKISMHFEKEMGIPIDMEFTYETQEKKMTLVQLRPLSSYEEMASVR
                     IPEVSHDKILLKGSRMVSNGIIESTPYLVYVDPFRYGNRENYFKIARALGKVNERLKG
                     TRYVLIGPGRWGSVNPDLGVPVDYAELCNCGCMVELGIVEKHFTPELSYGTHFFLDLD
                     VDNILYLPVFAGEKPDIFNRDWFEEHPYETGSEEEIRVYKGNFSVYLNGETEEG"
     gene            complement(1705..2004)
                     /locus_tag="PK146_07245"
                     /pseudo
     CDS             complement(1705..2004)
                     /locus_tag="PK146_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582826.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
BASE COUNT          428 a          537 c          551 g          488 t
ORIGIN      
        1 ctccctcctc ggtctctcca ttcagatata ccgagaagtt ccccttgtag acccgtatct
       61 cttcttcgga gccggtttcg taggggtgct cctcgaacca gtccctgttg aaaatatccg
      121 gtttttcgcc cgcaaagacg ggcaggtaaa ggatgttgtc cacgtcgagg tcaaggaaaa
      181 aatgggtccc ataggagagc tcaggagtga aatgcttttc cactatgcca agttcgacca
      241 tgcagccgca gttgcatagt tcggcgtagt cgacgggcac accaaggtcg ggattgacgc
      301 ttccccatct tccgggtccg atcaacacgt accttgtccc cttgaggcgc tcattcacct
      361 ttcccagagc cctggctatc ttgaagtagt tctccctgtt gccgtatctg aaagggtcaa
      421 cgtagacaag gtagggtgtc gattcgataa ttccgttgct gaccatcctt gaccccttga
      481 gaagtatctt atcgtgggac acctctggaa tcctgaccga cgccatttct tcataggagg
      541 agagtggcct gagttggacc agggtcatct ttttctcctg ggtctcgtag gtgaactcca
      601 tatcaatagg tatccccatc tccttctcga agtgcatgga gatcttcctg gcagtctcga
      661 ggaagtgggg gtaacgggtg ggaagatcgc caaaagtgaa aaaaggagta gttgggatca
      721 ttggtctcga gcccgcccag taaaggatgt tgtcgccaaa cagctctaca aatgccgtgg
      781 ccgacttgtg atgttccatg atgaagggga aaagggatct caggtcacct gagacgaagg
      841 cgccgaactc aagatccacg tagtcaaaac tgctctggct gtaccggacc acctctctgg
      901 cgaggttccc ctctggcctc atgttggggt gtgacaggta gaccaccctg gcaagggacc
      961 tgtcgacggt cctggtcccc agtccaaaac agaacctgat caccccctgc tccttgtcta
     1021 tccttggtga aggtctccgg tagaccctgg agaagatcgt tccagccagt tcggggtagt
     1081 agagcccgtc atggtcgtgt cctataaggg gttgtaccag gacggccatt gcttcgtccc
     1141 ggtccgcaaa gccgtgtttg gtgcgatatg cccttgctgc ctggttgtag agcgaggccc
     1201 aaacggtctt gatgccgtcg gccagttccc gccgcctcca ctccatgtcg aatacgttgg
     1261 ccgcaaaggt ggtgaagtac ttacccgcaa aggacagaag atgggagtcc tcgagaaggg
     1321 agctggacct tatggccagg ggagtgtcgt agagggtctg gagaactttg tcgagatcgt
     1381 tgagaaagga ctctctcaac ctcccctggg agaaggcgag aatgatcttt tccaggtcgg
     1441 gttcatcgtg gatccagtcc ataacgttgt cgttcaaaaa gtcgtggaaa ggttcggtgg
     1501 taatgacata ggtcatcgag ggcagcttga cgtcctccat gagctctgag ccctggagat
     1561 gatcaaaggc gaaggacaaa ccctttgcct tgccgccgat actgccgctc ccgatcagcc
     1621 agttgcgctg ttcgtagttg ggtcgtccat caaaatcctt gtaagggatc catttgttgg
     1681 gaaccatctc cagctctcac tcccttatgg gttaagtcgg tatattgttc tcgggaaggg
     1741 aatggtctcc ctgatatggg ggaggccgca gacccatgcg acaaccctct ctattcccat
     1801 cccaaagccg ctgtgtacga atgtgccgta tctcctcagg tcaaggtacc agccgtatga
     1861 gtcctcagga agtttctcct cgattattct tttgtgaagg agttcgaagg aatcctccct
     1921 ctgtgagccg cctatgatct ccccgtaacc ctccggtgcc aggaggtcgt cgcagaggac
     1981 cagatcctcc tttcagggtg ctgc
//
LOCUS       DAOPKQ010000244         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_66462_length_1996_cov_4.735703, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000244 DAOPKQ010000000
VERSION     DAOPKQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66462_length_1996_cov_4.735703"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1996
                     /locus_tag="PK146_07250"
     CDS             <1..>1996
                     /locus_tag="PK146_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPE92070.1"
                     /translation="QARKCGFDEKEYLEALAKVPRISRAKTEWAMSFFVKGADYISEL
                     GVRNLQLARVLEKNSVLLEHLATSEALLKIAGHLVSLGGWQFDIAENRLIWSDEVAEI
                     HEMPPGYTPNVDDGIGFYAPEYRERITEVFARCVETGEPYDEEMQIITGTGRRVWVRT
                     IGEAVRNGEGKIVKVQGGFQDISEQKMAEEALRRSEAQFRALVEGAPDAIYVHIDGRF
                     VYLNETALRMYGAKSPEEILGRCILELFHPSMHDVVRERLHTINVERKSVPTMEQIHL
                     RLDGGEIPVEVTSIPIIYDGKESALVFARDISERKEAREALRESEERFRSIFENATIG
                     LYRTSPDGKILMANPKLVAMLGYSSFEELSSRNLDEVGFEPGYPRSCFQEMIDRDGEI
                     RGLESAWTRRDGSVIFVRESARAIRDLSGRVLCYEGTVEDVTAQRRSEEELRKKEDLI
                     RFAGKLASLGGWSVDLPGNRTVWTDEAARIHDLPSDYSADVEEAKGFYAPGSRALIDR
                     VYAECVKNGIPYDEEFRKVTSTGRSIWVREIGEAVRDETGKIIRVQGAFQDITEWKRA
                     EEFLRRSEEKYRQITENMSDVVWTADLGMNTTYVSPSVRKLLGETPEEHLVRGMRDKF
                     PPEDLAKLMSVLEEELERERSGDAPKDRSRIVEVQHYRADG"
BASE COUNT          492 a          489 c          642 g          373 t
ORIGIN      
        1 caggccagga aatgcggctt cgatgagaaa gagtaccttg aagccctggc aaaggtccct
       61 aggatatccc gcgcaaagac agaatgggcc atgtcctttt tcgtcaaggg agcggactac
      121 atctccgagc tgggggtaag gaaccttcag ttggcgcggg tcctggagaa gaacagcgtt
      181 cttcttgaac atcttgcaac gagcgaagcg cttttgaaga tcgccggaca tctcgtctca
      241 cttggaggat ggcagttcga cattgccgag aaccgtctca tctggtccga tgaggtggcc
      301 gagatccatg aaatgccccc tggctacacc ccaaacgttg atgatggcat cgggttttac
      361 gctccggagt accgtgaaag gataaccgag gtctttgcca ggtgtgttga gactggtgag
      421 ccatacgatg aggagatgca gatcatcacc ggaaccggcc gacgggtatg ggtaaggacc
      481 atcggggagg ctgtccgcaa cggagaaggc aagatcgtga aagtgcaggg gggctttcag
      541 gacatttcgg aacagaaaat ggcggaagag gctctcaggc ggagcgaggc gcagttcagg
      601 gcgctggttg agggcgctcc tgacgccatc tatgtccaca tagacggcag gttcgtctat
      661 ctcaacgaaa ccgcattgag gatgtatggt gccaagtccc cggaggagat actgggaagg
      721 tgcatcctcg aactgttcca cccatccatg cacgatgtgg tccgggagag actccacacg
      781 ataaatgttg aaaggaagtc cgtccccacg atggaacaga tccatctgcg gctcgacgga
      841 ggagagatac ccgttgaggt tacttccata ccgataatct acgacgggaa ggaatcagcc
      901 cttgtctttg cgcgggatat ctctgaacgt aaagaggccc gggaggccct gagggagagc
      961 gaggaacgtt tcaggagcat cttcgagaac gccaccatcg gtctttaccg gacatcgccc
     1021 gatggcaaga tcttgatggc caatcccaaa ctggtggcga tgttgggcta ctcctccttc
     1081 gaggaactct cctctcgtaa cctcgacgaa gtgggtttcg agccggggta ccctcgaagc
     1141 tgtttccagg agatgatcga ccgggatggt gagatcaggg gcctcgagtc agcctggaca
     1201 agacgggacg gttccgtgat cttcgtcagg gagagcgcca gggcgatccg ggacctttcc
     1261 ggaagggtcc tctgctacga gggtaccgtg gaggatgtta cagcccagag gaggtccgag
     1321 gaggaactga gaaagaagga agaccttatc cggttcgcgg gcaaactggc ctccctgggg
     1381 ggctggagcg tcgatttgcc gggcaaccgc acagtctgga ccgatgaggc ggcaaggatc
     1441 catgacctcc cctccgatta ttcagccgac gtagaagagg caaagggttt ctacgcaccg
     1501 ggatcccgcg ctcttattga cagggtctac gcggagtgtg taaagaacgg aattccctac
     1561 gacgaggagt tcaggaaggt aaccagtacc ggcagaagca tatgggtgcg tgagataggg
     1621 gaagcggtaa gagacgagac aggaaagata ataagggtgc agggcgcctt ccaggatata
     1681 actgaatgga aaagagccga ggaattcctt cgccggagcg aggagaagta ccgccagatc
     1741 acagagaaca tgtccgacgt ggtatggacg gcagatctcg ggatgaatac cacctacgtc
     1801 agtccatcgg tcaggaagct gttgggagaa acacctgagg aacaccttgt caggggtatg
     1861 agggacaagt tcccgcctga ggacctggcc aaactcatgt cagttctcga ggaggaactg
     1921 gaacgggaga gatccggcga tgccccgaag gatcggtcca ggatcgtgga ggtgcagcac
     1981 tacagggccg acggga
//
LOCUS       DAOPKQ010000245         1977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_67423_length_1977_cov_4.209677, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000245 DAOPKQ010000000
VERSION     DAOPKQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67423_length_1977_cov_4.209677"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK146_07255"
     CDS             <1..572
                     /locus_tag="PK146_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPE92071.1"
                     /translation="VVEAEGGRTMMTPSDLPTGGDRVAFAAREFGFVDCVLNIQGDDP
                     LVGPDMIDPLVSMMASRPEVSLGVLGKAIEDPSEESDPNIVKMVFDLRGRALYFSRST
                     IPFRRNPGASMYKHIGPYAWRRDFLLDFCRWEQTPLERSESLEMLRVLENGYDISCIE
                     TLRDTIEIDSPEDVEKLERFFREYGDDLK"
     gene            569..1402
                     /gene="kdsA"
                     /locus_tag="PK146_07260"
     CDS             569..1402
                     /gene="kdsA"
                     /locus_tag="PK146_07260"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPE92072.1"
                     /translation="MIKIFSAGKVLFGDGGLVLVAGPCVLEDREMALSIARRVSGICD
                     NLGIPYVFKASFDKANRTSIHSFRGPGLERGLEWLSDIRSSVGCPVLTDIHLPDQARI
                     AAEVVDIIQIPAFLCRQTDLVVAAASTGKVVNIKKGQFLAPADMGQAVSKCLEAGNDK
                     VILCERGSSFGYGQLVVDMRSLAIMRSLGYPVMFDATHSVQMPGARGTSSGGDRRFVP
                     VLARAAAAAGIDALFLETHPDPDSAKSDGPNMVSLDSLENLLKRVLAIHQVASGTEGE
                     K"
     gene            1405..>1977
                     /locus_tag="PK146_07265"
     CDS             1405..>1977
                     /locus_tag="PK146_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPE92073.1"
                     /translation="MVSLPRERNSIDYDDERLLEVGRSVLLVEAEEISRAASRVGKEL
                     VEAARMINRCEGRLVVVGMGKSGLVGRKIAATLASLGTPAFFLHAAEGAHGDLGMVCR
                     EDAGLFLSNSGQTREVLEVLPFFRRLGAPVIAMTGNTSSRLARDSDVVIDSGVVKEAD
                     PLGLAPTTSTTLQLALGDALAGMVTELRGLK"
BASE COUNT          398 a          540 c          642 g          397 t
ORIGIN      
        1 aggttgtcga ggccgagggc ggccggacga tgatgacccc ttcggacctt cccacaggcg
       61 gtgacagggt ggcctttgcg gccagggagt tcgggttcgt cgattgtgtc ctcaatatcc
      121 agggcgacga tcccctggtg ggacccgaca tgatcgaccc gctggtctcg atgatggcca
      181 gcaggcctga agtatccctg ggggtccttg gaaaggctat cgaggacccc tcggaggagt
      241 cggatcccaa catcgtcaag atggtcttcg atctaagggg cagagcgctc tatttcagcc
      301 gttccacgat acccttcaga aggaacccgg gggcctcgat gtacaagcat atcggcccct
      361 atgcatggag aagggacttc ctcctcgatt tctgccgatg ggaacagacc ccgctggaga
      421 gatccgagag cctggagatg ctcagggtgc tcgagaacgg gtacgatatc agctgcatag
      481 agacgttaag ggatactata gagattgatt ctcctgagga tgttgaaaaa ctggagagat
      541 ttttcaggga atatggagat gatctgaaat gatcaaaatc ttttctgcag gcaaagtcct
      601 ttttggtgac gggggtcttg ttctggtcgc cggtccctgc gtcctggagg acagagagat
      661 ggctctctcc atagcgcgaa gagtctcggg gatctgcgac aaccttggca taccctacgt
      721 cttcaaggcc tccttcgaca aggcgaacag gacctctatc cacagcttca ggggacccgg
      781 cctggagagg gggcttgagt ggctttctga tatccgaagc agcgtgggct gccccgtcct
      841 gaccgatatc catctccccg accaggcaag gatcgcagct gaagtggtgg acataatcca
      901 gatcccggcg ttcctgtgca ggcagacgga ccttgtagtc gctgcagcgt ccaccgggaa
      961 ggtggtgaac atcaagaagg ggcaattcct tgctccggcg gacatgggcc aggccgtctc
     1021 caagtgcctt gaagccggca acgacaaggt catcctctgc gaaaggggga gctctttcgg
     1081 ttacggtcaa ctggtggtcg acatgcgctc cctcgcgatc atgaggtccc tgggataccc
     1141 cgttatgttc gatgccaccc acagcgtcca gatgcccggt gcacggggga cttccagcgg
     1201 tggggacaga aggttcgtcc cggtccttgc ccgtgcggcg gcagcggcgg gaatagatgc
     1261 ccttttcctg gagactcatc ctgaccccga ctcggcaaaa agcgacggtc ccaacatggt
     1321 ctccctggat tctctcgaga acctcctgaa gagggtcctt gccattcacc aggttgcctc
     1381 cggaacggaa ggagagaagt gaaagtggtc agcctgccga gggaaaggaa cagcattgac
     1441 tatgacgacg aaaggcttct cgaggtcgga cggagcgttc ttcttgtgga ggccgaagag
     1501 atctccaggg ccgcctcccg tgtgggcaag gagctggtgg aagcggcgcg gatgatcaac
     1561 aggtgcgagg ggcggcttgt agtcgtggga atgggcaaat cggggctggt cggccggaag
     1621 atagctgcga cgctggcttc tttgggtacg ccggccttct ttcttcacgc cgccgaagga
     1681 gcccacggcg accttgggat ggtctgccgg gaggacgcgg gccttttcct gagcaacagc
     1741 ggccagacca gggaggtgct ggaggtcctg cccttcttca gaaggctcgg cgccccggtt
     1801 atcgccatga cggggaacac ctcctccagg ctggccaggg attccgacgt ggtgatcgat
     1861 tcaggcgtgg taaaagaggc cgatccgctg ggactcgctc ctacgaccag cacgaccctg
     1921 cagctggccc ttggggacgc tcttgcgggg atggtgacag agcttcgggg gcttaag
//
LOCUS       DAOPKQ010000246         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_67800_length_1970_cov_3.466841, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000246 DAOPKQ010000000
VERSION     DAOPKQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67800_length_1970_cov_3.466841"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..944
                     /locus_tag="PK146_07270"
                     /pseudo
     CDS             195..944
                     /locus_tag="PK146_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
     gene            941..1894
                     /locus_tag="PK146_07275"
     CDS             941..1894
                     /locus_tag="PK146_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPE92074.1"
                     /translation="MKILFYPRTFEKLQGIVGPLLEGHEIEVADLSDIKDRIRMADVL
                     VTGPLDVDEELIRGASRLRLVHQWGVGVERIDLEACFRKGIDVCNVPSEGTGNAEGVA
                     EIALMHMLLLSRRFGRCQESIRKRRLFSPQGVALWKKRACVIGLGNVGRNIVTRLKGM
                     GMDVTGVNRTERGSFGSLGLDSFCSLDRLQDALPGCRFVILSLELNEDTRGIAGEDFF
                     SSMDPGSYLINVARSELVSRTALESSLGRGHLAGAGFDVFWDEPADPEDPLLENSAVT
                     LTPHIGGVTDEALMGVARSVAENVTRLARDLDLMNRLDPGK"
BASE COUNT          447 a          504 c          621 g          398 t
ORIGIN      
        1 gatctgcccc cgggccagga gagcctggat ctctgggacc gctcccccgg gaagccgtca
       61 gggaaggggt ccgagaacag caagaccccc gagacacagg agaaggggta attacagatc
      121 ggaggggtgc cttccttacg cgggatgtac agagcgaggg tgatatccgg aacattcccg
      181 tcgacagcgt aggtgtgaca ggattggcct acccgatcga ggtcagggac cggaacgaaa
      241 agatccagca cgctttagct aaggtggaga tgtccgtctc tctctccagg gactaaaggt
      301 ggatacatat gagtcgtttt attgagatcc ctaacttttg caacggccac gtgatactgc
      361 cgaatcttga gcgtatcgcc gcgaccctga gggatcatct cgatgtggag caggccgaga
      421 taaggttcag cttcccctat tttgccgtga ggaaggctcc cgtaagcggg gcgctcagcc
      481 tgacccgata cgatgtggcc ttcctggcct caagcgggga agagttcgat ttcaccctta
      541 ctgtcatagt ccctgtgaac accctttgca catgttcgag gaagatctct gaaaaggggg
      601 ctcacaacca gcaagcagaa gtgacgatca ccgtcaggat ggaccgcctg gtctggatcg
      661 aggaactggc cgaaatcgca gaagagggcg catcgtcccc ggtcctcact cttctcaaga
      721 gcgaggacga gaagttcata accgaaaagg catatgatac ccccaggttc gttgaggacg
      781 tggtcagaga tgttgcggtg cgtctggacg atgaccccag gatcctctgg tattcggtaa
      841 gggttgtaag ccgcaagagc atacatgccc atgaggcctt tgcaagtctg tccagggaca
      901 agggtgtgtg gatcgatcag cagagagggg accgtcaccg atgaaaatac tgttttaccc
      961 aaggaccttc gagaaactcc agggtatcgt cggaccgctc cttgaggggc atgagataga
     1021 agtggccgac ctgtccgata tcaaggaccg gataagaatg gcagatgtcc tggtcaccgg
     1081 tcccctggat gtcgacgaag agcttatcag gggagcctcg aggctcaggc tcgtccacca
     1141 gtggggagta ggtgttgaaa ggatcgacct ggaagcctgt ttccggaagg gcatagatgt
     1201 gtgcaacgta ccttcggaag ggacaggcaa tgccgaaggg gtcgcagaga tagcgctgat
     1261 gcacatgctc ctgctctccc ggaggttcgg aagatgccag gagagcatcc gcaaaagaag
     1321 gctcttctcg ccccagggtg tggctctgtg gaaaaagagg gcctgcgtga taggcctggg
     1381 gaacgtcggt cggaacattg ttaccaggct gaaggggatg ggcatggatg tcaccggggt
     1441 taacaggacc gaacgagggt cattcggctc cctgggtctt gacagtttct gctccctcga
     1501 ccgcctgcag gatgcactgc cggggtgcag gttcgtcata ctctccctgg agctcaacga
     1561 agacaccagg gggatcgctg gcgaggattt tttcagttcc atggatccag gttcctacct
     1621 gatcaatgtg gccaggtccg aactggtatc ccggacagca ctggagagtt cactgggccg
     1681 gggacacctt gccggggcgg ggtttgatgt attctgggat gaacctgccg atccggagga
     1741 tcccctgctt gagaactccg ctgtcacact tacgccacac ataggtgggg tcactgacga
     1801 ggcccttatg ggcgtggcca ggtccgtggc ggagaacgtc acccggctgg caagagatct
     1861 ggacctgatg aaccgattgg accctggaaa gtgaaggctt taatggaccc gtcagcctcc
     1921 attgagaaac agggcgtgaa aggtgctgtc ctcttcgatt tcgacatgac
//
LOCUS       DAOPKQ010000247         1963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_68149_length_1963_cov_4.217505, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000247 DAOPKQ010000000
VERSION     DAOPKQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68149_length_1963_cov_4.217505"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1963
                     /gene="leuS"
                     /locus_tag="PK146_07280"
     CDS             <1..>1963
                     /gene="leuS"
                     /locus_tag="PK146_07280"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPE92075.1"
                     /translation="KWQKRWESEGIFNVERDPDREKFYCLEMFPYPSGALHMGHLRNY
                     SIGDMLARFLMMEGKQVLHPMGYDAFGLPAENAAIKYETHPHKWTWDNIEHMTDQLKR
                     MGCSYDWRRRVETCNPNYYHWTQWLFLQFHRKGLAYRKTAPVNWCESCGTVLANEQVI
                     NDGHCWRCATPVVKKNLEQWFLRITDYAQELLDSLEDLPGWPERVKLMQRNWIGRSEG
                     VRLRFALYGNSVEIETFTTRIDTIFGVTFLALSPEHPAVSFIADRSPKKDEIEEFVTS
                     SMAQTEREQAAAGGEKRGIDTGLFAVNPVNGERVPVWIANYILMEYGTGAIMGVPAHD
                     QRDFDFARKYSIPVIPVINPPTGEVLDGKTMDVAFEEDGIQCNSGKFDGMPSSEALPA
                     MTQWFVSSGWGEREINFRLRDWLISRQRYWGAPIPIIYCDKCGAVPVPESDLPVELPL
                     DIKMRTGVNPLATAEEWKRVPCPKCGGPGIRETDTMDTFICSSWYFLRFASPWTGKAP
                     FETDDTDYWMAVDQYIGGIEHACLHLIYARFFTKFLADCGMLKVREPFTNLLTQGMVI
                     KDGAKMSKSRGNVVDPDEIITKWGADTARLFILFASPPEKDLDWSDQGVEGAHRFLQR
                     VYRLVETNQEGLRGASKEVLPMASISDKKA"
BASE COUNT          438 a          591 c          593 g          341 t
ORIGIN      
        1 agaagtggca gaagcgctgg gagagcgaag gaatcttcaa cgttgaaaga gaccctgaca
       61 gggagaagtt ctactgcctg gagatgttcc cataccccag tggggccctt cacatgggac
      121 acctcaggaa ctactccata ggagacatgc tggcccggtt cctgatgatg gagggcaagc
      181 aggtccttca ccccatgggc tacgacgcct tcgggcttcc ggcagagaac gccgccatca
      241 agtacgagac ccacccccac aagtggacct gggacaacat cgagcacatg acggaccagc
      301 tcaagaggat gggctgcagc tacgactggc gcagaagggt cgagacatgc aaccccaact
      361 actaccactg gacccagtgg ctcttccttc agttccacag gaaggggctg gcctacagga
      421 agacggcccc cgtcaactgg tgcgagagct gcggtacagt ccttgccaac gagcaggtca
      481 tcaacgacgg tcactgctgg aggtgcgcca cgccggtggt gaagaagaac ctcgagcagt
      541 ggttcctccg catcacagac tatgcccagg agctgctcga ctcccttgag gaccttccgg
      601 gctggcctga gagggtcaag ctgatgcagc gcaactggat aggccgttcc gagggcgtaa
      661 ggctcaggtt cgccctctac gggaacagcg tggagataga gaccttcact acccggatcg
      721 acaccatatt cggcgtcacc ttcctggccc tgtcgccgga acaccctgcc gtgtccttta
      781 tcgccgacag gtctccaaaa aaggacgaga tagaggagtt cgtcacgtcc agcatggccc
      841 agactgaacg ggaacaggca gctgccgggg gcgagaagag gggcatcgat acgggtctct
      901 tcgccgtgaa ccccgtaaac ggcgagaggg tccccgtctg gatagccaac tacattctca
      961 tggagtacgg caccggggcc ataatgggtg tccccgccca cgaccagaga gacttcgact
     1021 ttgccaggaa gtactctatc ccggtcatcc ccgtcatcaa cccgcctacg ggtgaggtcc
     1081 ttgacggcaa gaccatggac gtagcctttg aggaggacgg gatccagtgc aattcgggca
     1141 agttcgacgg gatgccgtct tcggaagctc tcccggccat gacccaatgg ttcgtatcca
     1201 gcggatgggg tgagagagag atcaacttcc gtctcaggga ctggctcatc tcgagacagc
     1261 gatactgggg cgcgccgatc cccattatct attgcgacaa atgtggtgcc gtgcccgtcc
     1321 ccgagagcga cctgcccgtg gagctaccgc tggacatcaa gatgaggacc ggtgtgaacc
     1381 ccctggcgac ggcggaggag tggaagaggg tcccgtgccc gaagtgcggc gggccgggga
     1441 tccgtgagac cgacaccatg gacaccttca tatgctcctc atggtacttc ctaaggttcg
     1501 cctcgccctg gacgggcaag gcccccttcg aaacggatga caccgactac tggatggccg
     1561 tcgaccagta cataggcggt atcgagcacg cctgcctgca cctgatctac gccaggttct
     1621 tcaccaagtt cctggccgac tgcggcatgc tgaaggtgag ggagcccttc accaaccttc
     1681 tgacccaggg gatggtcatc aaggacggcg ccaagatgtc caagtccagg gggaacgtgg
     1741 tcgaccccga cgagataatt accaaatggg gtgccgatac tgcccgcctc ttcatacttt
     1801 tcgcctcgcc cccagaaaag gacctggact ggtccgacca gggagtggag ggggcccacc
     1861 gcttcctcca gagggtgtac aggctcgtcg agaccaacca ggagggactc cggggtgcct
     1921 cgaaggaagt ccttcccatg gcatccatca gtgacaaaaa ggc
//
LOCUS       DAOPKQ010000248         1945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_69027_length_1945_cov_5.117460, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000248 DAOPKQ010000000
VERSION     DAOPKQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69027_length_1945_cov_5.117460"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /locus_tag="PK146_07285"
     CDS             <1..1642
                     /locus_tag="PK146_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPE92076.1"
                     /translation="ARSSGTVTRVDSTSIEIQAGKSGKTDCYNLVKFRRSNQGTIIHQ
                     KPIVFKGDRVKAGDIIADGQAVENAELALGNNVLVAFVSWEGYNYEDAILLSDRLVKN
                     DSFSSIHIEEYEVEARDTKLGPEEITRDIPNVGEDALKDLDETGIVRIGAEVVAGDIL
                     VGKVTPKGESDQSPEEKLLRAIFGEKAREVRDTSMRIPHGEGGKVVAVKQLSRSENGD
                     ELTPGVNEVVKVYVAKIRKITVGDKMAGRHGNKGVVSRILPEEDMPYLPDGTPIDVVL
                     NPIGVPSRMNLGQVLETILGFIASTNDWHVATAVFEGATENEILDLVEKTRQESFPEL
                     TFDGRMLLYDGRTGEPMMRPVTVGTMYMMKLIHLVDDKIHARSIGPYSLITQQPLGGK
                     AQFGGQRFGEMEVWALEGYGASHVLQEILTVKSDDIRGRLKTYERIVKGLNLTDPGVP
                     ESFRVLVKELQGLGLDVEIAYEDGTLGELLLEEDDEPVSSPRTRPREEKREGKVLNMD
                     DVFEDIFPEELSKGGEQEPVFSVLEVDSDNDPEEGADE"
     gene            1643..>1945
                     /locus_tag="PK146_07290"
     CDS             1643..>1945
                     /locus_tag="PK146_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92077.1"
                     /translation="MNRRGISGVRIKLASPERIREMSSGEVKKPETINYRTLRPEKDG
                     LFCERIFGPTKSFECACGKYKRSGPKFKGVVCDRCGVEIADNRVRRERMGHIELAAP"
BASE COUNT          468 a          539 c          629 g          309 t
ORIGIN      
        1 ggcgcgcagt tccggcaccg tgacgagggt agattccacc agcatcgaga tccaggccgg
       61 caagagcgga aagacagact gttacaacct tgtcaagttc aggagatcca accaggggac
      121 catcattcac cagaagccca tagtcttcaa gggcgaccgg gtcaaagccg gggacattat
      181 cgccgacggc caggcggtgg agaacgccga gctcgccctg ggcaacaacg tccttgtggc
      241 gttcgtgtcc tgggagggct acaactacga ggacgccatt ctgctaagcg acaggctggt
      301 gaagaacgac agcttctctt ccatacatat cgaggagtac gaggtggagg ccagggatac
      361 caagctgggg ccggaggaga taacccgcga tatccccaac gtgggcgagg atgccctcaa
      421 ggacctcgac gaaacgggga tcgttaggat cggcgccgag gtcgtggccg gagacatcct
      481 ggtggggaag gtcaccccca agggtgagtc cgaccagtcc cccgaggaaa aactgcttag
      541 agccatcttc ggcgagaagg caagggaagt cagggacacc tccatgagga tcccccacgg
      601 ggagggcggc aaggtggtcg ccgtcaaaca gctcagccgc tccgagaacg gagacgagct
      661 taccccaggg gtcaacgagg tggtcaaggt atacgtggcc aagatcagga agatcaccgt
      721 cggtgacaag atggccggcc gtcacggcaa caagggcgtc gtctccagga tcctccccga
      781 ggaggacatg ccctacctgc ctgacggaac gcccatcgac gtggtcctga acccgatcgg
      841 tgtcccgagc cggatgaacc tcggacaggt tctggagacc atcctcggct tcattgcatc
      901 cacgaacgac tggcacgttg ccacggccgt gttcgagggc gcgacggaga atgagatcct
      961 tgacctggtg gagaagaccc gccaggaaag tttccctgag ctaacctttg acggaaggat
     1021 gctcctctac gacggaagga caggggagcc catgatgcgc cctgtcaccg ttgggaccat
     1081 gtacatgatg aagcttatcc acctggtgga cgacaagatc cacgcaaggt ccataggccc
     1141 ctacagcctc atcacccagc agcccctcgg cggaaaggcc cagttcggcg gccagcgctt
     1201 cggggagatg gaagtatggg ccctggaagg ctacggggcc tcccatgtac tccaggagat
     1261 actcaccgtc aagtcagacg atatcagggg gaggctcaag acctacgaga gaatagtcaa
     1321 gggcctgaac ctgaccgatc ccggggtacc cgagagcttc agggtcctcg tcaaggagct
     1381 ccagggcctt gggcttgatg tagagatcgc ctacgaggac ggcactcttg gggagctcct
     1441 tctcgaggag gacgacgagc ctgtctcctc gcccaggacc aggccgcggg aagagaagcg
     1501 ggaagggaag gtcctgaaca tggacgacgt attcgaggac atattccccg aggagttgtc
     1561 aaaaggtggg gagcaggaac ctgttttttc ggtcctggaa gtcgatagcg acaacgaccc
     1621 ggaagagggg gcggatgaat agatgaaccg aagaggtatt tcaggggtaa ggatcaagct
     1681 ggccagcccc gaaaggatcc gggagatgtc cagcggagag gtcaagaagc ctgagaccat
     1741 caactacagg accctcaggc ccgaaaagga cggcctcttc tgcgaaagaa tattcggccc
     1801 caccaagagc ttcgagtgcg cctgcggaaa gtacaagagg agcggtccga agttcaaggg
     1861 tgttgtctgc gaccgttgcg gcgttgagat agctgacaac cgcgtccgaa gggagcgcat
     1921 gggccatatc gagcttgccg cgccg
//
LOCUS       DAOPKQ010000249         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_69148_length_1943_cov_3.675847, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000249 DAOPKQ010000000
VERSION     DAOPKQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69148_length_1943_cov_3.675847"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PK146_07295"
     CDS             <1..259
                     /locus_tag="PK146_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPE92078.1"
                     /translation="TEDLVNALLSVRGVRAALLFQEDEGCCRVSIRTRDPLNAREMAA
                     LWGGGGHERASGCRMGETPERSLKAFLSRLEELDEIGLSGN"
     gene            234..1154
                     /gene="truB"
                     /locus_tag="PK146_07300"
     CDS             234..1154
                     /gene="truB"
                     /locus_tag="PK146_07300"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPE92079.1"
                     /translation="MRSGFLVIDKAPGTRSTQCTNLVKRILGGSVRVGHAGTLDSTAS
                     GILVLLVGRATRASRFVMMLPKTYLVTARLGSSTDTDDLSGQVIALGDAGKADEAGID
                     RALLSFSGLRDQVPPSFSAVKVKGRRAHSMARRGFDVRLEARPVLVTSIRRAGPLEGD
                     LEVPLGITCHQGTYVRSIVRDLGAALGCGAVVSSLTRTSLGPFILPDGIPSDDLAAMN
                     AHSLQERILPLSVISSSFTTFTAHPPEEDLILSGRPVPFERLERRGWGAYPGEGLVVI
                     TGRRVLSFARIEAGKGRTMGRPVTNILLEG"
     gene            1161..>1943
                     /gene="ribF"
                     /locus_tag="PK146_07305"
     CDS             1161..>1943
                     /gene="ribF"
                     /locus_tag="PK146_07305"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HPE92080.1"
                     /translation="MIAVIGSFDGFHLGHRSLFHAAEVISKKISDSWCVVTFFPHPQA
                     VLGRRAFLPLFTEPEKDILGSCLGVPEIVRIPFTRELADMDPDDFLDSLERSLFIRGL
                     VVGEDFRFGRNRSGDAALLRELASVHGWEAEIVPALATSSGRKIGSSFIRESITRGNL
                     SDASADLGHPFMVMGIVVHGDGRGKTIGFPTVNLSLTNEKLIPARGVYSGGVAVGTDV
                     FPAAINIGLNPTFPGERDLRCEAHIPGFRGDLYGKEVSLFFLR"
BASE COUNT          405 a          513 c          603 g          422 t
ORIGIN      
        1 tacggaggat ctggtcaacg cccttctatc tgtcaggggt gttcgcgccg ctctactctt
       61 ccaggaggat gaggggtgct gccgtgtaag catccgtaca agggatccac tgaacgcaag
      121 ggaaatggct gccttatggg gtgggggagg acatgaacgc gcctcgggtt gcaggatggg
      181 agaaacccct gagagatcgc tgaaggcctt tctttcaagg ctggaggaac ttgatgagat
      241 cgggctttct ggtaattgac aaggcgccgg ggacgagaag cacccagtgt accaacctgg
      301 ttaagaggat ccttggcggc agcgtgagag tggggcacgc gggtacactt gactcgacgg
      361 cttcagggat ccttgtcctt cttgtcggaa gggcgacaag ggcctcccgg ttcgtgatga
      421 tgctcccaaa gacatacctt gtaacggctc gccttggatc tagtacggac accgacgacc
      481 tttcagggca ggtcattgcc ctgggggacg caggcaaggc cgacgaggca ggcatagaca
      541 gggcgctgct ctccttttcg ggtctccggg accaggtacc cccgtccttt tcggccgtca
      601 aggtaaaggg cagaagggct cactcaatgg cacgaagggg cttcgatgtc cggcttgaag
      661 caagaccggt tctggtaacc tcgatcagga gggcaggccc ccttgaaggc gacctggaag
      721 ttcctcttgg gatcacatgc catcagggca cctacgtcag gagtatagta agagaccttg
      781 gagcagccct gggatgtggc gcggtggtct cgagcctgac caggacgtcc ctggggccct
      841 ttatcttgcc cgacggtatt ccaagcgatg atctggccgc aatgaatgcc cactccctgc
      901 aggagaggat ccttcccctt tcggtcatct caagcagttt taccaccttc acggcacacc
      961 ctcccgagga ggacctcatc ttatcaggcc ggcccgtccc ttttgaaagg ctcgagagaa
     1021 gagggtgggg cgcataccct ggagagggcc tggttgtgat caccgggaga agggtgctct
     1081 cctttgcaag gatcgaggcg gggaaaggca gaaccatggg cagaccggtc acgaacatac
     1141 tactggaagg atgaccactg atgatagccg ttatcggatc tttcgacggt tttcacctgg
     1201 gacacaggag tctcttccat gccgcggaag ttatctctaa aaagatctcc gattcctggt
     1261 gtgttgtgac cttcttcccc catccccagg cagttcttgg gcgcagggcc tttctgcctc
     1321 tgttcaccga gccagaaaag gatattttgg gcagctgtct gggggtgcct gagatcgtaa
     1381 ggataccatt taccagggag ctcgccgaca tggatcccga tgatttcctg gattcgcttg
     1441 agagaagcct tttcatcagg ggtcttgtgg tcggagagga ttttcggttc ggcaggaaca
     1501 ggtccgggga tgcggccctg ctcagggagc tcgcctccgt ccacggctgg gaggctgaaa
     1561 tagtccctgc ccttgccaca tcttccggaa gaaagatcgg gagcagtttc atcagggaga
     1621 gcataaccag gggcaacctt tccgatgctt ccgcggatct ggggcacccc ttcatggtaa
     1681 tgggaatcgt cgttcacggc gacggaagag ggaagaccat tggattcccg accgtgaatc
     1741 tgtccttgac aaatgagaag ctgataccgg ccaggggggt ctactccggt ggggtcgcag
     1801 tagggaccga tgtctttccg gcagctatca acataggtct taacccgact ttccccggtg
     1861 aaagggacct gagatgcgaa gcacatatcc ccggattccg gggcgatctt tacgggaaag
     1921 aggtgtcact tttctttctg aga
//
LOCUS       DAOPKQ010000250         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_69711_length_1932_cov_4.165157, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000250 DAOPKQ010000000
VERSION     DAOPKQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69711_length_1932_cov_4.165157"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK146_07310"
     CDS             <1..721
                     /locus_tag="PK146_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92081.1"
                     /translation="IGLSSEAYEIESSRAERSKSLACWCRRHQPLALRSWDTGLLRRM
                     DDQTLQSIMAYEGRDMGLAPLSRSKTSRLLGLIRRGEGRWRFQWERDLEICGSRRSIS
                     LVERSLFSSPGPCGESFPLKGERGSFSWGPWSFEWEIAPGGGPFLGERACRIPVNGSE
                     VIEVGAAGSPGETGPSRAGIPWWAEMVWPEVRTSGTVWVPFSGPSCTSRRQSAGKDGP
                     SLELRARIGGSGLKGEIQDGI"
     gene            711..1247
                     /gene="hpt"
                     /locus_tag="PK146_07315"
     CDS             711..1247
                     /gene="hpt"
                     /locus_tag="PK146_07315"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPE92082.1"
                     /translation="MGFDLGEVLIRRKSIQERVRELAFDIGSAYGDRDLVLIGVLKGA
                     VVFLSDLIRELPVSMTTELDFLAVTSYGTSTESSGVVRIIKDLDININGRNVIIVEDI
                     VDTGLTLSYLVSILKEREPEMLEVCSLLDKPSRRKTEIDIAFKGFEIPDEFVVGYGLD
                     YSGRWRNLPEIYTLKKRD"
     gene            1296..1883
                     /locus_tag="PK146_07320"
                     /pseudo
     CDS             1296..1883
                     /locus_tag="PK146_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
BASE COUNT          443 a          470 c          606 g          413 t
ORIGIN      
        1 tatcgggctc tcctcggagg cctacgagat cgaatcgtcg agagcggaac ggtcgaagag
       61 ccttgcctgt tggtgcagaa gacatcagcc actcgcattg aggtcgtggg acactggact
      121 gctgagaaga atggatgacc agacgttgca gtcgataatg gcatacgagg ggagagacat
      181 gggacttgcg cccctttcaa ggagcaagac ttccagactc ctcgggttga tccgcagagg
      241 agaggggaga tggcgattcc agtgggagag ggaccttgag atttgcggtt caaggaggtc
      301 gatatctctc gttgaaagaa gtctattctc ctcccccggc ccttgtggag agtcatttcc
      361 cctgaagggg gaaagaggat ccttcagctg ggggccatgg tcctttgagt gggaaattgc
      421 ccctggcggc ggtccttttc tgggagagcg ggcctgccgg atccccgtga acgggtcgga
      481 ggtaattgaa gtaggggccg ctggatctcc aggggagacg gggccatccc gggcagggat
      541 accatggtgg gccgagatgg tctggccaga ggtcagaaca tcagggactg tctgggtccc
      601 tttttctggc ccttcgtgta cctcaaggag acagtcggcc ggtaaagacg gaccctccct
      661 tgagcttcgg gcgaggatcg gaggaagcgg actgaaagga gagatccaag atgggatttg
      721 atctcggaga ggtcctgata aggagaaagt ccatccagga gagggtcagg gaacttgctt
      781 tcgatatagg ttccgcttac ggggacaggg atctggtgct gatcggcgta ctgaagggtg
      841 cagtggtctt cctttccgac ctgataagag agctgcctgt atccatgacg acggaactgg
      901 acttcctcgc ggtgacatcc tatggaactt ccaccgaatc cagcggcgtt gtaaggataa
      961 tcaaggacct ggatatcaac atcaacggca ggaacgtgat cattgtcgag gatattgttg
     1021 acacggggct aaccctctcc tacctggtca gcatcctcaa ggaaagagag ccggagatgc
     1081 tggaggtctg ttccctgctt gacaagcctt ctaggagaaa gaccgagatc gacatagctt
     1141 tcaaggggtt cgaaatcccc gacgagtttg tggtaggcta tggcctggac tattccggga
     1201 gatggaggaa cctgcccgag atctacaccc tgaagaaaag ggactaggcc cttttccagg
     1261 agtacctgtt cttttgaacg agaggtggga cagtattggg aagaatggct aagaacctcg
     1321 ggctttacct tgtaatgatc gtccttgtgg taagcctggt gaacatgttc ctttcaccgg
     1381 cccagagccc agaggaacat gaactgataa gctatagcgc cttcctgggg tatttccggg
     1441 aagggaaact ggaacaggta tccatcgagg gcgacaatat caagggtacc cgggagggag
     1501 gggtcaaatt ccgcaccatc gcggtcggga tcggcgacct ggcaccccag ctggcagaaa
     1561 agggtgtaca ggtggaggtc cagcctccgg caagatcccc ctggtgggta acgatgatgt
     1621 cctccctgtt ccccaccctg cttctgatag gagcctgggt attcatcctc tatcacatgc
     1681 agggcggcag caacaaggtg atgagttttg ccaagaacaa ggccaagctt ttcctcgaca
     1741 acaggcccaa ggtgaccttc gccgacgtgg ccggctgtga cgaatccaag gaggaactca
     1801 aggaggtcgt tgactatctg agggatccag accggttttc caggcttggg gccaagatac
     1861 cgaaaggggt gcttcttctg ggtccctcag atagtcaacg acctccttga gttcctcctt
     1921 ggattcgtca ca
//
LOCUS       DAOPKQ010000251         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_70918_length_1909_cov_3.757821, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000251 DAOPKQ010000000
VERSION     DAOPKQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70918_length_1909_cov_3.757821"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PK146_07325"
     CDS             <1..562
                     /locus_tag="PK146_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92083.1"
                     /translation="TIRELWRDVVEGDRFPRLLRVLLAVILIAGTIWSAMLFRQMILT
                     AQARDVPPPPTGTAATDKEIESLKETAAGFRNAVLARSGSTQLTVLAATLARRPFSPS
                     EPEAALTEEGEIAVAQAPPNIWIKAVLIRGKEAAVVADIEGYGDGVILKRGTSFGGGL
                     GRVLGISQDKVVVTWSGQQVDIPVDR"
     gene            559..>1909
                     /locus_tag="PK146_07330"
     CDS             559..>1909
                     /locus_tag="PK146_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HPE92084.1"
                     /translation="MKRALGVLFLTVLLLSPFSALAASAQEAGADEEMGPAPPAILGL
                     TLVQGGSTLAVFQVTGEKLPVPDVLSTDENGLVLLWEGVILPSGYWERSFPTPLVQGV
                     VLRQEPEGVQMQIRSTRRVYLGKTVGDPPSPEMQLHLTTGDVAEEIPLPVTGVPKVVA
                     GDPFGSSAPVTLQLRDSDLRDVFRMLGSMVNMNVVIDPSVPATPVTISLNNVPMNEAF
                     QYLMRMYDVSYAIMGKTIIVGRKDNLSRAMGMEKTRAFRIAYADLGQIPALLQGLSGV
                     SSVVVDERLRTVYVTASEERLGDVAVTLQRIDHPGRQVMLQARIVEVSEGGSDELKGL
                     VNAVYERWYASFSSAGGVIGYYDDYNGGVFNPSRTNREINPPESITFPDMADSALSFF
                     DASLQILVKQDKAKMLASPSVVTLDGQKASIKLLTDIKYVSERDDQGRPTYGEVGSGP
                     "
BASE COUNT          385 a          559 c          608 g          357 t
ORIGIN      
        1 aaccatcagg gaactctgga gggacgttgt cgagggggac cgcttccccc ggcttctcag
       61 ggtgcttctg gcagtgatcc tgatagcggg aacgatctgg tcggcgatgc tcttcaggca
      121 gatgatcctt accgcccagg ccagggatgt gcccccgccg ccgacaggta cggccgcgac
      181 ggacaaggag atcgaatccc tcaaggagac cgcggccggt ttcaggaacg ccgtcctcgc
      241 aaggtcgggg agcactcaat tgaccgtcct ggccgccacc ctggcaaggc gacctttctc
      301 accgtcggag cccgaggcgg ctttgaccga agagggtgag atagcggtcg cccaggcgcc
      361 gcccaatata tggataaaag ctgtgctgat cagggggaag gaagcggccg tggttgccga
      421 catagaaggt tacggcgacg gcgtgatcct caagaggggg acctccttcg gaggaggcct
      481 cggaagggtc cttggcattt cacaagacaa ggtggtggtc acatggtccg gtcagcaggt
      541 agacattccc gtggacagat gaaaagggcc ctgggtgttc tcttcctgac ggtccttctg
      601 ctctccccat tttccgcact ggccgcgtcg gctcaggagg ctggggccga cgaagagatg
      661 ggaccggccc ctcccgccat tttgggactg acgctcgttc agggaggttc cacgctggcg
      721 gtattccagg taacggggga gaagcttccc gtccccgacg ttctgtctac cgacgagaac
      781 ggcctggtcc tcctctggga gggtgtgatc ctcccgtcag gttactggga gaggtccttc
      841 ccgacgccgc tggtacaggg ggttgtcctc cgccaggagc ccgaaggggt ccagatgcag
      901 atacgctcca ccaggagggt atacctcgga aagaccgtgg gcgacccacc cagtccggag
      961 atgcagctgc acctcaccac cggagatgtt gcggaagaga tacccctccc cgtaacgggc
     1021 gttccgaaag tagtggcagg tgaccccttt ggatcatcag ctccggtcac ccttcagctt
     1081 cgcgactcgg acctgaggga tgtcttccgc atgctgggat ccatggtgaa catgaacgtg
     1141 gtcattgacc cttcggtccc ggcgacgccg gtgacgataa gcctcaacaa tgtgcccatg
     1201 aacgaggctt tccagtacct gatgaggatg tacgacgtat cctacgccat tatgggcaag
     1261 accatcatcg ttggcaggaa ggacaacctc tcccgcgcca tggggatgga gaagacccga
     1321 gccttcagga tagcctacgc tgacctcggc cagataccgg cccttttgca gggactgtcg
     1381 ggagtctcaa gcgtggtcgt cgacgagagg ctcaggaccg tctacgttac agcctcggag
     1441 gagaggctag gggatgtggc cgtaactctc cagaggatag accatccggg gaggcaggta
     1501 atgctccagg ccaggatcgt ggaggtctcc gaaggcggct ccgatgagct caagggcctg
     1561 gtcaacgctg tctacgagag gtggtacgcc tccttctcca gcgccggcgg tgtgataggc
     1621 tactacgacg actacaacgg aggtgttttc aacccttcca ggaccaacag ggagatcaat
     1681 ccccccgaga gcattacctt tccggacatg gctgacagcg ctctcagttt cttcgacgcc
     1741 agcctgcaga tcctggtgaa gcaggacaag gcaaagatgc ttgccagccc gtcggtggtc
     1801 acccttgacg gccagaaggc ctcgatcaag cttcttacgg acatcaagta cgtctcagag
     1861 cgcgatgacc agggtaggcc cacctacggg gaggtggggt cgggaccgt
//
LOCUS       DAOPKQ010000252         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_71434_length_1899_cov_3.594902, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000252 DAOPKQ010000000
VERSION     DAOPKQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71434_length_1899_cov_3.594902"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK146_07335"
     CDS             complement(<1..246)
                     /locus_tag="PK146_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92085.1"
                     /translation="MGKKTRRFQDLFRLCRNERRFSQLKTLAVIPARYGSTRLPGKPL
                     IRIGGIPLVLRVFLQASRCSSITDVIVATDDERIVQVV"
     gene            complement(174..>1899)
                     /locus_tag="PK146_07340"
     CDS             complement(174..>1899)
                     /locus_tag="PK146_07340"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPE92086.1"
                     /translation="FGNGRLMPAGMLRESPRSLIRAHMVVITKADQVPPDRVREIAAT
                     IQEISGRSPFLSSLALAGWSRFLGPREGLRDSGMPGLPVAAFSAIGNPVSFGSFLEKL
                     GFPTIFHEAFRDHHIFSEEELDVICRRVISSGARSLVCTEKDIFNLPQEWSPPLPVYV
                     PRVRAVLGDERSFISALSHYLQPKVVVSSNGHGEDAIGSLLAERLKKKFPLSDVSGFP
                     IVGRGQEYRSRGISVLSPPSETPSGGVVKYRLRDLVRDIRSGLLRHIGAQAEAWSGLR
                     GKVRTVICVGDVYLFLHTLWGQGAMPVLLATAKSVRLHGHWGLEKHLIRLRCRRVFTR
                     DPETARELSERKADAVYRGNPIMDLASDTIKRSEGKRPFRVLLLPGSRERAYEDLVLL
                     LEAAAEIAKEEDCRFEMVVASTLDRKRLLGKVPSWMGVNGDLIRHDKGGPEVRLFFGE
                     VSEAASRSDILVGLGGTANQICAGMGVPVVSIDEKGKRVQKKLLGEAESLVPAKPALL
                     AREVLSILGDPVLRESMSRAGRERMGHGGAVDALVEYASEVLGWAKRHDVFRTFSDFA
                     ETKGDLAN"
BASE COUNT          394 a          602 c          519 g          384 t
ORIGIN      
        1 gacaacctgg acgatccttt cgtcgtcggt agccacaatg acatccgtta tggaagaaca
       61 ccgggacgcc tgaagaaaga ccctcaacac caggggtatc ccccctatcc tgataagggg
      121 tttcccaggt agacgggtgc taccgtaccg ggcaggtatg accgcaagag tcttcaattg
      181 gctaaatctc ctttcgtttc ggcaaagtct gaaaaggtcc tgaaaacgtc gtgtcttttt
      241 gcccacccaa gaacctcaga agcgtattcg accagggcat ccacggctcc accgtggccc
      301 atcctctccc tgccggccct ggacatgctc tcgcggagga cagggtctcc caggatggag
      361 aggacctctc tcgccaggag ggccggtttg gcgggcacca gagactctgc ctctccaagc
      421 agttttttct gtaccctctt gcccttctcg tcgatggaga ccaccggcac gcccatgccg
      481 gcacagatct ggttggccgt acctcccagc ccgacaagga tgtccgaacg ggatgccgct
      541 tcagagactt ccccgaaaaa gaggcggacc tcagggcctc ccttatcgtg tctgataagg
      601 tctccgttaa cgcccatcca ggaagggacc tttcccagca gtcttttcct gtcaagggtc
      661 gaggccacga ccatctcgaa ccggcagtcc tcctcctttg cgatctctgc agcagcttcg
      721 aggaggagga ccagatcctc gtaagctctc tccctgctgc cggggaggag caggaccctg
      781 aagggtctct tgccttcaga ccttttaata gtatcactgg caagatccat tatcgggttt
      841 cccctgtaga cggcatcggc cttcctctcc gacagctcac gggctgtctc cggatccctt
      901 gtgaaaaccc ttctgcacct cagcctgatc agatgcttct ccagacccca gtggccgtga
      961 aggcgaacgg attttgccgt ggcaagaagg accggcatcg ctccctgccc ccagagggtg
     1021 tgcaggaaaa ggtatacatc ccctacacag ataactgtcc tgaccttccc cctgagacca
     1081 ctccaggcct ccgcctgggc accgatatgc cggagaaggc ctgaccggat gtcacggacg
     1141 agatctctca gacgatattt gaccaccccg ccgcttggcg tctcgcttgg aggagacagc
     1201 acactgatgc ccctggaccg gtactcctgg cccctcccga ctatggggaa accggagaca
     1261 tcggagaggg ggaacttctt tttcagcctt tccgcaagga ggcttcctat ggcatcctct
     1321 ccgtgcccgt tggatgagac caccaccttc ggttgcagat agtgcgagag agccgaaata
     1381 aatgatctct cgtcgcccag tactgctcgg accctgggaa cgtaaacagg aaggggcggt
     1441 gaccattcct gtggaaggtt gaagatatcc ttttcggtgc agaccagcga ccgggctccc
     1501 gaggagatca ctctcctgca gatcacgtcc agttcttctt cgctgaagat gtgatggtca
     1561 cggaaggcct cgtggaaaat ggtcgggaat cccagcttct ccaggaagga accgaagctc
     1621 acagggtttc ctatggcaga aaaggccgcc acggggagtc cgggcattcc ggagtcccga
     1681 agcccctccc ggggaccaag gaaccttgac caccctgcca aagcaaggga ggagagaaag
     1741 ggtgacctcc cggatatctc ctgtatcgtg gcggcgatct ccctaacccg gtccggcggc
     1801 acctggtcgg ccttggtgat caccaccatg tgggccctga tcagcgaccg ggggctctcc
     1861 ctgagcatcc ctgccggcat cagccgcccg ttcccgaag
//
LOCUS       DAOPKQ010000253         1892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_71807_length_1892_cov_3.853566, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000253 DAOPKQ010000000
VERSION     DAOPKQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71807_length_1892_cov_3.853566"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..1064)
                     /gene="prxU"
                     /locus_tag="PK146_07345"
     CDS             complement(348..1064)
                     /gene="prxU"
                     /locus_tag="PK146_07345"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930177.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(813..815),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HPE92087.1"
                     /translation="MEEKEIGCARPTGALVGEPEPAPEEKAAPEKKEVQGMSIMVGRK
                     APDFEAPVYHKGKFGSVKLSDSLGKWVVLCFYPGDFTFVUATEVSAVADKYPVFQELG
                     VDVYSCSVDSQFVHKVWNDNELSKMVEGGVPFPMISDGGGNLGKIYGVYDEKAGVNVR
                     GRFIIDPDGVIQGMEVLTPPVGRNVEETIRQIKAFQLVRETKGKEVTPSGWQPGKTTL
                     KPGPDLVANVWKFWKPKDNK"
     gene            1226..>1892
                     /locus_tag="PK146_07350"
     CDS             1226..>1892
                     /locus_tag="PK146_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPE92088.1"
                     /translation="MELSSSLASERLYAEDLDRKDDLASFRERFSLIPGAVYMDGNSL
                     GLCPRDAEKAIAGTVEEWKGLGIRGWMEGNPPWFEMAERVGGDAAPLVGADPDEVVLA
                     GTTTVNLHALVSTFYEPTPERSVILADELNFPSDLYALGSQVALRGFDPDKSLRLAGS
                     PDERTLDEEKVIGEMKPDVCLVVLPSVLYRSGQLLDMERLAMAAHDRGIPIGFDCAHS
                     AGAI"
BASE COUNT          421 a          512 c          508 g          451 t
ORIGIN      
        1 cacacatata gtttttccgg ctgccctgcc cataaggggt cacttatggc gaaagcgaca
       61 agaccaagga taaacaaaag cgcgataaag ataacaggct tcttcatcgg ttcatcactc
      121 ccctttcaag gtatacctct atgggtatat aagaagtcta tatcctgatc aggaacaatg
      181 gatgagtagg atcgcctata tccgggggtt tgaaaaaaag atcgccccct ggcagggggc
      241 gatccggtcc tgttcacgat actgtaactt gcctttcctg tcgcggtttg aggtctttcg
      301 cctatctttt atgtacacaa ttgctgcaat tcaaggtctg accccaatta cttgttatcc
      361 ttgggtttcc agaatttcca gacgttggcg accaggtccg ggccgggctt gagggttgtc
      421 tttccgggct gccaccccga gggtgtgacc tctttcccct tggtctcgcg gacaagctgg
      481 aaggccttga tctggcggat ggtctcttca acgtttcttc caacgggagg agtcagaacc
      541 tccataccct ggataacacc gtcggggtcg attatgaaac ggccgcggac attgactccg
      601 gccttctcgt catagacacc gtagatcttt ccaaggtttc cgccgccatc ggagatcatc
      661 gggaagggca ctccaccttc taccatcttg gagagttcgt tgtcgttcca aaccttgtgt
      721 acgaactgac tgtcaacact gcaggaataa acatccactc caagttcctg gaatacgggg
      781 tatttgtcgg cgaccgccga cacttcagta gctcagacga aggtgaaatc acctggatag
      841 aaacagagga ctacccattt cccaagtgaa tcagacagct tcacgctgcc gaacttgccc
      901 ttgtgatata ccggtgcctc aaaatcaggg gcctttcttc ctaccattat cgacattccc
      961 tggacctcct ttttttctgg tgcagccttc tcttccggag ccggttccgg ctctccgaca
     1021 agggctcctg tcgggcgggc acaaccgatc tctttctcct ccaacgaaca cacctcccga
     1081 tggtatttac ctcaaaatga aataattttg gatatcattt cccggtaaca tgatagcata
     1141 ttcttcatgt ttaatgtgag agaatatttt atacagaacc agatcttcgg ggtgccggaa
     1201 agatcccgcc cccctacgga ggtgcatgga actgagctca tctctggcat cggaaaggct
     1261 ttacgcggaa gatctcgaca ggaaggatga cctggcctct ttccgggaga ggttctccct
     1321 tatccccgga gccgtataca tggacggcaa ctctcttgga ctatgcccca gggatgcgga
     1381 gaaggccatc gcgggtaccg ttgaagagtg gaaaggactg ggcattcgtg gttggatgga
     1441 gggcaacccc ccgtggttcg agatggcaga gagggtcggg ggtgatgctg cgcctctagt
     1501 gggagcggac cctgatgagg tggttctggc aggaacgacg acggtcaacc ttcacgccct
     1561 ggtatccacc ttctacgaac cgacgccgga gaggagcgtt attctcgctg atgaactgaa
     1621 tttcccatcg gacctctacg cccttgggag ccaggtggcc ctgagaggct tcgacccgga
     1681 taaatccctg aggcttgcag ggtccccgga cgaaaggacc cttgacgagg agaaggtcat
     1741 cggggagatg aagcccgatg tgtgcctggt agtcctgccg tcggtcctct acaggagcgg
     1801 gcagctgctc gacatggaga ggcttgccat ggcagcccat gataggggca taccgatagg
     1861 gtttgactgc gcccattcag caggggccat cc
//
LOCUS       DAOPKQ010000254         1861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_73511_length_1861_cov_5.993909, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000254 DAOPKQ010000000
VERSION     DAOPKQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73511_length_1861_cov_5.993909"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..945
                     /gene="fapR"
                     /locus_tag="PK146_07355"
     CDS             334..945
                     /gene="fapR"
                     /locus_tag="PK146_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HPE92089.1"
                     /translation="MERSAARKIRQTRIISELEANPLLTDEEIAGRLGVSVSTVRLDR
                     AMLGIPEVRERMRSMANQAGSRLRSLKREEVIGELLDLQPGEWALSTLQTSREMAFRH
                     THFVSDHYIYAMASSLAIAVIEADMVVTGSARVRFRSPARVGERLVARAKVGTKKGNK
                     NVVSVRIRVGEQEIFVGRFITVEKECLNGSFESERCDPDVAGS"
     gene            929..>1861
                     /gene="plsX"
                     /locus_tag="PK146_07360"
     CDS             929..>1861
                     /gene="plsX"
                     /locus_tag="PK146_07360"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPE92090.1"
                     /translation="MLLALDVMGGDNAPLETCAGAVMACERFTDLELALVGDTEAVRP
                     LLDKVPASVRTRLHLVHAEELITMDEPPAVAVRKKRHSSLRIAMQMVRSGEAQGCVSA
                     GSTGAIVAGGVLVVGRIPGIERPGLGVPLPAVNRISMLIDIGATVRCKPLNLYQFALM
                     GDVYMRKIIGVESPSIGLLSNGQEEVKGDELIIEAREFFRKSRLNFIGNVEGGTIPFG
                     AADVVVCEGLTGNILLKFIEGTGEAMYSLVREEISRHFLAKVGMLFMIPMLKDLWARF
                     NYEETGGTPLLGVNGAVIKAHGRSRARAICGAVRV"
BASE COUNT          418 a          474 c          572 g          397 t
ORIGIN      
        1 gatcctgatc cccttcccaa cgggttcatt accgaaagac cgagcaagat cagggggcga
       61 attccccccc ctgatctttt ttgtgcccaa ctttagaggt gacgcaactc gccctcttgg
      121 cgtcgtccgt cagtccctat gttccttata atgacctccg ctcggatggt cacggttccc
      181 tggccctgtt cctattctcc ttcgggaggt ccttccccgg tgtttcccac ccgccacatc
      241 ctgttcattt catattgact aacaaggtcc ggaacattat acttcctgat catataacct
      301 gatgttagca ccagatagca aaggagaaga gccatggaaa ggtctgctgc cagaaagatc
      361 cgccagacca ggatcatctc ggagcttgag gccaaccctc tccttacaga tgaggagatc
      421 gccgggagac tgggtgtaag cgttagcact gtcagactgg acagggcgat gcttggtatc
      481 cccgaggtca gggagcgtat gaggtcaatg gcaaaccagg ctggtagcag gctcaggtcg
      541 ctgaaaaggg aggaggtcat cggtgagctc ctggacctcc agcctggcga atgggccctc
      601 tcaactctcc agacatcgcg agagatggcc ttccgccaca cccatttcgt ttcggaccac
      661 tacatctacg ccatggcgag ttccctggca atagccgtca ttgaagcgga catggtggtc
      721 accggctcag caagggtccg gttcaggtct cccgccagag tgggcgagag gcttgtggcc
      781 agggcgaaag tcggtacgaa aaagggaaac aaaaatgttg ttagtgtgag aattagagta
      841 ggagagcagg agatattcgt cggaaggttc attacagtag agaaggaatg tctgaacgga
      901 tcctttgagt cagagaggtg tgatcccgat gttgctggct cttgatgtaa tgggagggga
      961 caatgctccc cttgagacct gtgctggagc ggtaatggct tgtgaaaggt ttactgatct
     1021 ggagttggcg cttgtgggag acacggaggc tgtcaggccc ctgttggata aagttcccgc
     1081 ctccgtcagg accaggctcc acctggtcca cgcagaagag ctgataacca tggatgagcc
     1141 ccctgcggtg gccgtgagga agaagagaca ttcaagtctt cggatagcca tgcagatggt
     1201 ccgttccggg gaggcccagg gctgtgtttc cgcggggagc accggtgcca tagtcgcggg
     1261 aggtgtactt gtcgttggac ggatccccgg aatagaaaga cccgggctgg gagtccccct
     1321 tccagccgtg aaccggatat cgatgctgat tgacatcggg gcgacggtcc ggtgcaaacc
     1381 cctgaacctc taccagttcg ccctgatggg tgatgtttac atgaggaaga taatcggggt
     1441 agagagcccc tccataggac ttctctctaa cgggcaggag gaggtcaagg gggatgaact
     1501 gatcatcgag gcgagagaat tcttcagaaa gagtcgcctc aacttcatcg gaaacgtgga
     1561 aggcgggaca attcccttcg gtgccgccga cgttgtggtt tgcgaagggc tgacggggaa
     1621 catcctgctc aagttcatcg aggggaccgg ggaggcgatg tactctctgg tccgggagga
     1681 gatcagcagg catttcctgg caaaggtggg aatgctgttc atgataccca tgctcaagga
     1741 cctatgggca cggttcaact acgaggagac aggaggaacg cctctccttg gggtcaacgg
     1801 ggccgtcatc aaggcccatg gaagatccag ggcaagggcc atatgcggtg cggtcagggt
     1861 c
//
LOCUS       DAOPKQ010000255         1834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_75092_length_1834_cov_4.671726, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000255 DAOPKQ010000000
VERSION     DAOPKQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75092_length_1834_cov_4.671726"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /gene="mutL"
                     /locus_tag="PK146_07365"
     CDS             complement(<1..1458)
                     /gene="mutL"
                     /locus_tag="PK146_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPE92091.1"
                     /translation="MKPIRQLSPDVSSRIAAGEVIENPASVVKELLENSLDAGAARIT
                     VELKDGGKTFIVVEDDGSGIPYGELPLAVERFATSKITEIDDLSDIGSFGYRGEALAS
                     VCAVSRLEIRSRRQGEEGGMLRAEGGSVSLHVTLPCRQGTRIQVEDLFFNLPARRSFL
                     KGSATEARRAIKVVREYSAAFPGVAFRALSENREVFSTDGCLERVEVLRKVWGTSETP
                     RHYIVASGPLRVETVWYPSPGSRRRDSIIFFNGRRVRDATVSAALSSCGEGASGNWMF
                     FITVPARLLDVNVHPGKAEVRCHSSLPVFDTVRRSVLDLVEGGFMTAEGMRTLSDLPV
                     NRGLVRRAGSGSFQGPPSEEGLFNRVGEPPFSPSIDAGGRREAGAGRFICRIGSGYLL
                     FEESEGLLIIDPHAAHERILFERISSRQSEAIPQKIPLPVDLPPSLSERAEGSRTELE
                     ELGFRFGQGQGSITLESVPGGSSNNGFLDPLDLLRS"
     gene            complement(1455..>1834)
                     /locus_tag="PK146_07370"
     CDS             complement(1455..>1834)
                     /locus_tag="PK146_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPE92092.1"
                     /translation="SGRLPGVKNLSMAVEETGEGIVFLYKVVPSPADRSYGIEVARLA
                     GIPKRVLERSMEILEEFEKNNGSVQVRPPRQTAGQLPLFSEDAEGLILELAGVEPDVM
                     TPIQALQLLYSLRDRATGVLRSR"
BASE COUNT          368 a          590 c          495 g          381 t
ORIGIN      
        1 ggatctcaga agatccagcg ggtcaaggaa cccgttgttg gaggacccac cgggtaccga
       61 ttccagggta atgctcccct ggccctggcc gaaccggaac cccagctcct ccagttcggt
      121 tcttgaacct tcagccctct cagagaggga ggggggcaga tcgacgggta agggtatctt
      181 ctgcgggata gcctcggact gcctgcttga gatcctctca aagagtatcc tttcatgggc
      241 ggcatgagga tcgataataa gaaggccttc gctctcctcg aaaaggagat atccagatcc
      301 gatccggcaa atgaaacgcc ccgctccggc ctctcttctc cctccggcat cgatcgacgg
      361 cgaaaaggga ggttcgccca ccctgttgaa gagaccctcc tcgctgggag gcccctggaa
      421 agatccggaa cctgcccttc ggaccaggcc tctgttcacc gggaggtccg aaagggtccg
      481 catcccttca gcggtcatga agcccccctc aacgaggtcc agaacggacc tccttaccgt
      541 atcgaagaca ggaagggagg agtggcagcg tacctccgcc ttgccggggt gtacgttcac
      601 atcgagaagt cgggccggta cggttatgaa gaacatccag ttccccgagg cgccctcccc
      661 gcaggacgaa agagcagcag agacggtggc gtcccgaacc cgccttccgt tgaagaatat
      721 tatggaatcc cttctcctcg atcctggcga aggataccat actgtctcca ccctcaacgg
      781 ccccgatgca acgatgtaat gccttggcgt ttcactggta ccccagacct ttctgagcac
      841 ttccaccctt tcgaggcatc cgtcggtgga gaacacctcc cggttttcgg agagggcacg
      901 aaaggctact cccgggaagg cagccgaata ctccctgacc acttttatcg cccttcttgc
      961 ctccgtggcg gaccctttca aaaaactcct tcttgcgggg aggttgaaga agagatcctc
     1021 cacctggatc ctggtcccct gtctacaggg aagggtaacg tgaagactga cgctcccgcc
     1081 ctcggctcgg agcattcccc cctcttcgcc ctgccttcgg ctcctgatct ccagtctgct
     1141 cacggcgcag acgcttgcca gcgcctcccc cctgtagccg aaggatccga tatcggagag
     1201 gtcatcaatt tcggtgatct tgcttgtggc gaacctctcc acggcgagag ggagttcccc
     1261 gtaaggaatg ccggaaccgt cgtcctcaac gacaatgaag gtcttgccgc cgtccttcag
     1321 ttccactgtg atcctggctg cccccgcatc gagagagttc tcaagaagtt ccttgaccac
     1381 ggaagccggg ttctcgataa cctccccggc agctatacgg gaagaaacgt ccggcgatag
     1441 ctgtctaatg ggtttcatct tgacctcaac acccccgttg cccggtccct cagggagtac
     1501 aacagctgga gagcctggat cggggtcatc acgtcaggtt ccactccggc aagttcaagg
     1561 atcagccctt cggcatcctc gctgaacagc ggtagctgtc ccgccgtctg acggggtggc
     1621 ctcacctgaa ctgacccgtt gttcttttcg aactcttcca gtatctccat ggacctttcc
     1681 aggacccttt tggggatacc tgccagcctg gccacctcga tcccgtagga tctgtccgcc
     1741 ggggacggca ctaccttgta aaggaagact atgccttcgc cggtctcctc gacggccatg
     1801 ctgaggttct tgacacccgg gagcctaccg gaga
//
LOCUS       DAOPKQ010000256         1814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_76337_length_1814_cov_5.146674, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000256 DAOPKQ010000000
VERSION     DAOPKQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76337_length_1814_cov_5.146674"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK146_07375"
     CDS             <1..483
                     /locus_tag="PK146_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92093.1"
                     /translation="QAQARKSPLLDLTWTGGDAVAQSTVILRDAGEAAAKVSVISPMY
                     SAPKSSKREELLAYAQDKIGETLDIYSLVAYDCAWLAALSAICTQSGDGEAIKDALPH
                     VAANYYGASGWTDLDELGDRKALNVEFFAVDEVDGELAWVKVSEYDAATGGLSEAPSK
                     "
     gene            558..1319
                     /locus_tag="PK146_07380"
     CDS             558..1319
                     /locus_tag="PK146_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPE92094.1"
                     /translation="MPEAMLEVCNVKKSFGGLKAVDGVSMSLPRGGITLLIGPNGSGK
                     TTFVNSITGFLHPDGGHVYLEGKEITGWPMYRICKEGMVRSFQLPAPFGGLSILGNLL
                     AACECGPGESLLWSPFKDMWRTKERENVREAFAVARAMELTSYWDAPMAETSTGHIKL
                     TEIGRALLAGAKLVILDEPICGVDPVMADEVFSFLVRLRDEQDLTFLVIEHRLDIALK
                     YADYVYVMNQGTLIAQGSVDEVLNNRQVKEVYLGS"
     gene            1316..>1814
                     /locus_tag="PK146_07385"
     CDS             1316..>1814
                     /locus_tag="PK146_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPE92095.1"
                     /translation="MSQLLRVQDLNAGYGAIHVLFDINLECDQGQAVAIVGPNGSGKT
                     TFLNSIFRLADIFSGRVVLDGKEIQREKAHRITSYGIGYLRQEGNVFPGLTVEENLKM
                     GAFNLPGKEVKERMDEVLSLIKPVEVFLRRTAGTLSGGEREMVALAAALMCRPKLLLL
                     DEPTSG"
BASE COUNT          421 a          477 c          537 g          379 t
ORIGIN      
        1 caggcgcagg ccaggaagag ccctctcctt gaccttacat ggacgggcgg cgacgctgtt
       61 gcgcagagta ccgtgatcct tcgggatgcc ggggaagcgg ctgccaaagt ctctgttatc
      121 tcgcccatgt actctgcccc gaaatcctcc aagcgggaag agctccttgc ctatgcccag
      181 gacaagatag gcgagaccct ggatatctac agtctcgttg cctatgattg cgcctggctt
      241 gccgcactgt ctgccatatg cacccaatcc ggtgacggag aggcgataaa ggatgccctg
      301 ccccacgttg ctgcgaatta ctacggggcc agcggctgga ccgatctgga cgaactcggc
      361 gacaggaaag cgctcaacgt agaattcttc gctgttgatg aagttgacgg agaactagcc
      421 tgggtaaagg tttcagaata cgacgcggcc acaggaggcc tctccgaagc accatcgaaa
      481 tagttgaatt agtggccctg tcccttaaca gggacagggc ccatttctct atttgatccc
      541 cagacggagg aatcaagatg cctgaggcaa tgctggaagt gtgcaacgtc aagaaaagct
      601 ttggcgggct caaagcggtg gacggtgtca gcatgtccct gccgcggggg ggtatcactc
      661 tcctgatcgg gcccaacgga agcggcaaga ccaccttcgt gaacagcatc accggctttc
      721 tccatcctga tgggggacat gtttaccttg agggaaagga aataaccggt tggccgatgt
      781 acaggatctg caaagaggga atggtgcgtt cttttcagct tccagctcca ttcggggggt
      841 tgagcattct ggggaacctt ctcgccgcct gtgaatgcgg ccccggagaa tccctcctgt
      901 ggtccccctt caaggatatg tggagaacaa aagagagaga gaacgttcgg gaggcctttg
      961 cagtggccag ggccatggag ctgacttcct actgggacgc cccaatggcg gaaacctcca
     1021 caggacacat caaacttacc gaaataggac gcgctcttct ggccggggca aaactcgtca
     1081 tcctggatga acccatatgc ggtgttgatc ctgtgatggc ggacgaggtc ttctcgttcc
     1141 tggtccggct aagggatgag caggacctca ccttccttgt tatagaacac agactcgata
     1201 tcgccttgaa atacgccgac tatgtctacg ttatgaacca gggaacgctg atcgcgcagg
     1261 gttcggtgga cgaggtgctg aacaatcgcc aggtaaaaga ggtgtatctg ggatcatgag
     1321 ccaattattg cgagtccagg acctcaatgc gggttatggc gccattcatg tccttttcga
     1381 catcaacctg gagtgtgacc aggggcaggc tgtggctatt gtcggcccta atggaagcgg
     1441 caagacgact ttcctgaaca gcattttccg cctcgcggac atattttcag gcagagtggt
     1501 ccttgacggt aaagagatcc aaagggaaaa ggctcacagg atcaccagct acgggatcgg
     1561 ttacctgaga caggagggta acgtctttcc cgggctcacc gttgaagaga acttgaagat
     1621 gggagcattc aatctgccag ggaaggaagt caaggagcga atggatgagg tgctctccct
     1681 cattaaaccc gtcgaggtgt tcctgcgcag gaccgcaggg acgctaagcg gcggggaaag
     1741 ggaaatggtc gcgctggctg ccgctcttat gtgcaggccg aaactccttc tcctcgacga
     1801 acctaccagc ggtc
//
LOCUS       DAOPKQ010000257         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_76864_length_1806_cov_5.326099, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000257 DAOPKQ010000000
VERSION     DAOPKQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76864_length_1806_cov_5.326099"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK146_07390"
     CDS             <1..571
                     /locus_tag="PK146_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92096.1"
                     /translation="SARNLEWKDMSSPYIRLVRGVVEALSGNHQATETLSGLPAQGMG
                     GDAQAMLCSARASIRDWEEARRMGRMALNSENGPFMWERQARRSREEAGYMASIAEAH
                     LGDWSAARDGFRRSASNLRHPGPVFALGVSLVALGKIDEAKVIQGQLRSSSVNLSKKL
                     EELIVQNSGIRKMLADPVDPGLLDLYAII"
     gene            651..1205
                     /locus_tag="PK146_07395"
     CDS             651..1205
                     /locus_tag="PK146_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPE92097.1"
                     /translation="MEILQEHNSEISHIRHALAVESAMRFFARLNGENEEEWGLAGLV
                     HDIDWETTNNDPSRHTHLGASWLRDAGYPDSIVRAVLAHGWGICSDVEPVSLMEKYLF
                     AIDELTGFVTAVALVRPGRSLQDLTVKSVKKKWKDKAFARGVDRDVIEKGTAMLEKPL
                     EELIEGVIEALRPVEKEIGLGEEA"
     gene            complement(1202..1672)
                     /locus_tag="PK146_07400"
     CDS             complement(1202..1672)
                     /locus_tag="PK146_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92098.1"
                     /translation="MKEKVPVLLALLMILLMGVSGVIFMRYLEVSEQNRVLREELLQA
                     TGEIHRIREEMPSESLPEGFPQPDWDDLRKRKIPDPRSWLVESLVSRRDLIPWEGIHG
                     GTMKIYDPSGVWFIGPRWCIAWVEDGHMGGYMLLRFQEDDGKPQWQLLDSELSD"
BASE COUNT          425 a          474 c          529 g          378 t
ORIGIN      
        1 cagcgccaga aaccttgagt ggaaggacat gtcaagccct tatattcgcc tcgtcagggg
       61 tgttgtggag gcgctttcag ggaaccacca ggcaacagag accctttctg gcctgcccgc
      121 ccagggcatg ggcggggatg cccaagccat gctgtgttcg gccagggcct ctatcaggga
      181 ttgggaagag gccagaagga tggggcgaat ggcacttaac agcgagaacg gtcccttcat
      241 gtgggagaga caggcaagaa ggtcccgtga agaggccggg tacatggctt ccatagcaga
      301 ggctcatctt ggagattggt ccgcggcacg ggatggcttc aggagaagcg cctccaacct
      361 caggcacccc gggccggtct ttgccctggg ggtttcgctg gtagcccttg gcaagataga
      421 tgaggcaaag gtcatacagg gtcagttgag atcctcttcg gtaaacctgt ccaaaaaact
      481 ggaggaactg atcgtccaga acagcggcat aaggaagatg ctggccgatc cggtcgatcc
      541 agggcttctt gacctatacg cgatcatctg atgtcccaag ggatcgatgg taacaatgat
      601 gataaaaagg gaggccacag ctcgtgaaac agtttgacag aaatgatgca atggaaattc
      661 ttcaggagca caacagtgag atcagccaca tccgccacgc ccttgccgtt gaatctgcca
      721 tgaggttttt cgccaggctc aacggagaga acgaggagga gtgggggctc gccgggcttg
      781 tgcacgacat agactgggag accaccaaca acgatccgtc caggcacacg caccttggcg
      841 cttcctggct gagggatgcc gggtatcctg acagtatcgt gagggctgtg ctggcccatg
      901 gctgggggat ctgctccgac gtggagcctg tctctctcat ggagaagtat ctgtttgcca
      961 tagatgaatt gaccggtttc gtcactgccg ttgccctggt aaggcctgga aggtctttgc
     1021 aggacctgac ggtcaagtct gtaaagaaga aatggaagga caaggccttc gccagggggg
     1081 ttgacaggga tgtcatcgaa aagggtacgg ccatgctgga aaaacccctg gaggaactga
     1141 tagagggcgt tatcgaagcc ctgagacctg ttgaaaagga gataggcctg ggcgaggaag
     1201 cctaatcgga caactcggaa tccagcaatt gccattgggg tttgccatca tcctcctgga
     1261 accggaggag catatagcct cccatgtgtc cgtcctctac ccaggcgatg caccacctgg
     1321 gcccgatgaa ccatacccct gaggggtcat atatcttcat agtcccgccg tggatcccct
     1381 cccaggggat caggtccctc cttgagacga ggctttccac caaccaggac ctcgggtcgg
     1441 ggatcttcct tttcctgaga tcatcccaat ccggttgggg gaagccttcg ggcaggcttt
     1501 ccgagggcat ctcctccctg atcctgtgga tctccccggt ggcttgcagc agttcttccc
     1561 ggagtaccct gttctgttcg ctgacttcaa ggtatctcat gaagatcaca cctgaaaccc
     1621 ccatcagcaa gatcatcagc agcgcaagaa ggacggggac cttttctttc atagccttgc
     1681 cctcccagac caaatcctag ggtatttgtg gtattgtctc atattaagat actctaatag
     1741 tgtaacatcg aactgtaaag gagagaggcc ccggaatgaa gaaacctctg acagctccag
     1801 aattga
//
LOCUS       DAOPKQ010000258         1795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_77543_length_1795_cov_3.828736, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000258 DAOPKQ010000000
VERSION     DAOPKQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77543_length_1795_cov_3.828736"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK146_07405"
     CDS             <1..551
                     /locus_tag="PK146_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPE92099.1"
                     /translation="LACFQDQLFAASEVAKEVAHRRDAFSAPGRGPVADFVGGEIRII
                     RKPNRPPALEGAIMPAKDVEILWASLGGGDWTLSCLSRSRSLEGLVLAGFGAGNIPPS
                     WIQYLKPLIKNDTAVVITTRCRRGHTNTLYGFEGSARRLLEMGVMDGGGLRPEHARLR
                     LAVGLGAHFSKEDLQLYLMGKK"
     gene            complement(599..1351)
                     /locus_tag="PK146_07410"
     CDS             complement(599..1351)
                     /locus_tag="PK146_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPE92100.1"
                     /translation="MSFFMLVLATLLGGVTGFLNVVAGGGSLLSLPFLIFLGLDASSA
                     NATNRVGILLQNVVAVWQFRRDKVLSLRESLSLGIPASIGAVGGALLAVQLDERFMKM
                     IIALLISFMAVLLVARPRMWETHRKTSWPTWAKWCLFIAIGAYGGFIQAGVGFLLIWA
                     LAGAVGKDLVRANALKVAIVLCFTVVSFIIFFSKGLINMQFGLALALGSMAGGWIGTR
                     FSVVKGNKWIRYILVVMVVVSALRMIFGAFGS"
     gene            1470..>1795
                     /locus_tag="PK146_07415"
     CDS             1470..>1795
                     /locus_tag="PK146_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPE92101.1"
                     /translation="MRYSVTEDMAFLRLSEGEELHRSISAACEEYSLDSAIIVGGLGM
                     AREIIFGWYTGKEYIRERFEGTFEVASLSGNVSMREGALYPHVHAVFNGMDHASLGGH
                     VLKVVT"
BASE COUNT          439 a          503 c          519 g          334 t
ORIGIN      
        1 tcctggcatg tttccaggac cagcttttcg ctgcttcgga ggtcgccaag gaggtggccc
       61 atcgaaggga tgccttctcg gctcccggga gaggccctgt ggccgatttc gtgggcggtg
      121 agatcaggat aatcaggaag cccaacagac ccccggcgct tgaaggagct ataatgcccg
      181 caaaggatgt ggagatactt tgggcatccc tcggcggagg ggactggacc ctttcatgtc
      241 tctccaggtc cagaagcctc gagggacttg ttctggctgg tttcggtgcg ggcaacatcc
      301 ccccctcgtg gatccagtac ctcaaacctc tgatcaagaa cgatacggcc gttgttataa
      361 ccacgcgatg tcggaggggg cacaccaaca ctctctacgg cttcgaggga agcgcaagaa
      421 gactgcttga aatgggcgtg atggacggag gagggctgag gccggagcat gccaggctaa
      481 ggctcgccgt aggcctggga gcgcactttt caaaggaaga cctgcagctt taccttatgg
      541 gtaaaaagtg aaaagagccc ggccgggggg tgcccccccc ggccgggctc ttttcccgtc
      601 agctcccaaa ggccccgaag atcatcctca gggcactgac aaccaccatt acaacaagaa
      661 tataccttat ccacttgttc cctttcacca ccgagaagcg tgtccctatc catcccccgg
      721 ccatactgcc caaggccagg gcaagaccga actgcatgtt gatgagcccc ttggaaaaga
      781 agataatgaa gctgaccacc gtaaagcaga gaacgatcgc caccttaagg gcattggccc
      841 tgacaagatc cttcccgacg gcccctgcaa gtgcccagat cagcaggaaa cccacccctg
      901 cctggatgaa acctccataa gcccctatgg cgatgaagag acaccacttg gcccaggtgg
      961 gccatgaagt cttgcgatgg gtctcccaca tccgcggcct ggcgacaagg aggaccgcca
     1021 tgaaggatat caggagggct atgatcatct tcataaagcg ttcatcaagc tggacagcca
     1081 ggagggcccc ccccaccgca ccgatggatg ccggaatacc cagggagagg gactctctca
     1141 acgacagcac cttgtccctc cggaactgcc acacggccac tacattctga aggagtattc
     1201 ccacccggtt ggtggcattg gcggaagaag cgtcaagacc gaggaagata aggaatggaa
     1261 ggctaaggag ggatccgccc ccggcgacaa cgttcaggaa acccgtaacc cctcccagca
     1321 gggtggcaag aacaagcatg aaaaagctca aatcggactc ctccatgaaa atacgggata
     1381 aaggacaggc gatattctaa cacatgatcc ccgaacaaca tcgcaatgat atggtaaaat
     1441 gatgtcacaa aggaagggag gagtgccctt tgcgatactc cgtaactgag gacatggcct
     1501 ttcttcgact gagcgaaggc gaggagctgc atcgctcaat atcagcggcc tgcgaggagt
     1561 actccctgga ttcggcgatc attgtcgggg ggttggggat ggccagggag atcattttcg
     1621 gatggtacac cgggaaggag tacatccggg agcggttcga aggaactttc gaggttgcgt
     1681 ccctgagcgg caacgtaagc atgagggagg gggctctcta cccccatgtt catgccgttt
     1741 tcaacgggat ggatcacgct tcgctgggtg gacacgttct gaaggtcgtt acctt
//
LOCUS       DAOPKQ010000259         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_78528_length_1779_cov_5.115429, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000259 DAOPKQ010000000
VERSION     DAOPKQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78528_length_1779_cov_5.115429"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..748)
                     /locus_tag="PK146_07420"
     CDS             complement(110..748)
                     /locus_tag="PK146_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPE92102.1"
                     /translation="MTIFLGAVDDDTGILYTLEAMASLQGWEMKTTTRAGEALDWIRK
                     DLVDMLLVDFHMPGMSGLEVIREARKISSETVLLVLTVEESPELAESLHMAGADDFIT
                     KPLRLADFTSRIALHAELGRYRREGRRDHPRKGLSEVIARKVLEIVSSGGNPTDVRTV
                     AEKAGISYPSAHRYLEFLVSKGSIAKKSLNVDGRPGRPRSIYHLPLKDAPRE"
     gene            complement(745..>1779)
                     /locus_tag="PK146_07425"
     CDS             complement(745..>1779)
                     /locus_tag="PK146_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPE92103.1"
                     /translation="HFGMPAVLGLASVLLVQYLTRGVTRWINRAVALVPLLVSFQWLD
                     VIPSLTRYGFGWGEISMAIKQIAELPNKTLVLDLVGVTFFLFLFAGALLTIELLVSYE
                     RQLEQFRRFRTQERELYSLREQQLQSRAVKEMQNLVHDLRRPLTTIVGLSDVIFEEGS
                     DKSRRHASVILKASRSMDQMISEILDAASARTVTVSALVDYSLSQVSPMDWSPSIEAD
                     IPHECGEVLLNVNAVRLSRALANLLDNAARASSPSPEGARIRLTARKSRDSVEISVED
                     NGPGFKQRPKAHRSGWGSTGLGLVFVQEVLRDHGGKVTFGKSSLGGASVTLSLQLYGC
                     GGEDKESARD"
BASE COUNT          358 a          531 c          507 g          383 t
ORIGIN      
        1 gagaaggccc aggggtttgg cccagaggtc gaagaaggca aaatctctca ccggtggatt
       61 gatccccagt atcctgcacc ctttcaggga tcccaggtat ccggagaggt cactcacggg
      121 gcgcgtcctt caagggcaga tgatagatgg accggggcct tcccggtctg ccgtccacgt
      181 tcaggctttt tttggcgatg cttcccttgg agacgaggaa ctccaggtat cgatgagccg
      241 aagggtagga gatgcctgcc ttttccgcaa cagttctgac atcggtaggg tttccgccgc
      301 tgcttacaat ttcaaggacc tttcttgcaa tgacctcgga aagacccttc cggggatggt
      361 cccgacgccc ctctcgacgg tatctgccca actctgcatg aagggctatc cgggaagtga
      421 aatccgccag cctgagaggt ttggtgatga agtcgtcagc tcctgccatg tgaaggctct
      481 ccgcaagttc tggtgactcc tcgacggtaa gaaccagcag gaccgtttct gaggagatct
      541 tcctggcctc cctgatcact tccaggccgc tcattcccgg catgtggaag tcaacgagca
      601 gcatgtccac caggtctttc ctgatccagt cgagcgcttc cccggctctg gttgtcgtct
      661 tcatctccca tccctgcagg gaggccatgg cctccagcgt gtagaggatg cccgtatcgt
      721 catctactgc ccccagaaaa attgtcaatc cctggcactc tccttatctt cccccccgca
      781 tccgtacagt tgcagggaaa gggtaaccga agccccgccc agggagcttt tcccgaatgt
      841 caccttgcct ccgtggtctc tgagcacctc ctgaacgaaa accagaccca gtccggtgga
      901 accccatcct gaacggtgtg ccttcggtct ctgtttgaaa cccggaccgt tgtcttcaac
      961 tgatatctcg acgctgtccc gtgattttct tgcggtcagg cggatcctcg ccccctccgg
     1021 tgatggggat gaagccctgg ccgcattgtc gagaaggttc gccagggccc tggagagcct
     1081 tacggcgttg acgttcagga ggacctcgcc gcattcgtgg gggatgtccg cctcaataga
     1141 ggggctccaa tccattgggc ttacctggct cagggagtaa tccaccaggg cggagacggt
     1201 cacggtacgg gccgaggcgg cgtcgaggat ctcggagatc atctggtcca tggacctgga
     1261 tgccttcagt atcaccgagg cgtgcctgcg ggatttatcg gatccctcct caaagatcac
     1321 gtccgaaagg ccgactatgg tcgtaagggg gcgcctcagg tcgtgaacaa ggttctgcat
     1381 ttccttgacc gccctggact ggagctgctg ctccctgagc gagtaaagct ctctctcctg
     1441 ggtcctgaaa cgccggaact gttccagctg tctctcgtag cttacaagaa gctctatggt
     1501 gagaagcgct cccgcaaaga ggaacaggaa gaaagtaacc cccaccaggt ccaggacgag
     1561 ggtcttgttc ggcagctccg cgatctgctt gatcgccatg gagatctctc cccagccgaa
     1621 tccgtatctt gtaagggaag gaatgacgtc aagccactgg aaagatacca ggagggggac
     1681 aagcgctacg gcgcggttga tccatctggt cacgccccgg gtaagatact ggaccaacag
     1741 gacgctggcc aggccaagca ccgccggcat tccgaaatg
//
LOCUS       DAOPKQ010000260         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_78922_length_1773_cov_4.647846, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000260 DAOPKQ010000000
VERSION     DAOPKQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78922_length_1773_cov_4.647846"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PK146_07430"
     CDS             <1..691
                     /locus_tag="PK146_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HPE92104.1"
                     /translation="ATCTRSSSDYIASDQFLEWMLDLGCKVGWFFQYIPTGDDPDLSY
                     MATPSQRLTLHRKVTEWRQKYPIFLGDFWNDGPFVDGCMAGGERYLHVISNGDVEPCV
                     FVHFAVDNINDRSLLDVIQSPFFQDIRERQPYDDDNLLCPCMIIDHPQVLREVVRTHG
                     ARATHPGSERILTELSEGLDSYSGNIHELFDPLWDGGGRERYLRSLEREDKPRPRGRL
                     NKRLQTEQRTG"
     gene            737..1123
                     /locus_tag="PK146_07435"
     CDS             737..1123
                     /locus_tag="PK146_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPE92105.1"
                     /translation="MKSYALAIAMAMLLAAVYAFQNTGEILVKFLVWELSFPQGIWEV
                     MLFAAGGVLMWLVSLFAMVESRSKYVRQIKEKDAKIREMEGERSSLMEAIRHSGPQYE
                     AEAQIRHSEVTHEEPAEEPGVEQEGQ"
     gene            1120..>1773
                     /locus_tag="PK146_07440"
     CDS             1120..>1773
                     /locus_tag="PK146_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPE92106.1"
                     /translation="MIGRIPVSRLMLREVDDDARTLAESVPCSPLTALLLKMRGISED
                     DPHEARAQLNPGLGDSMGRLDLGPFSSPAGDLWRSLEGAERVVVYGDYDADGACATAL
                     AMELALAAFREVRYFIPHRHREGYGVHEKVVRAIARNGCDLLIAVDCGTRDIAALRAA
                     REAGIPVIVFDHHSPGESLPEGALVVNPHAGGGPEAQTLCATGVLWAWARSEGIAAPE
                     "
BASE COUNT          392 a          486 c          557 g          338 t
ORIGIN      
        1 cgccacctgc accaggtcca gttccgacta catagcctcc gaccagttcc tggagtggat
       61 gctggatctg ggctgcaagg ttggttggtt cttccagtat atccccaccg gggacgaccc
      121 ggacctgagc tatatggcca ccccttcaca gagactgacc cttcaccgga aagttaccga
      181 atggaggcag aaatacccca tattcctggg cgacttctgg aacgacggcc ctttcgtgga
      241 cggatgtatg gcaggggggg agcgctacct ccacgtgatc tccaacggcg atgtcgagcc
      301 atgtgtcttt gtccatttcg cagtggacaa cataaacgac agaagcctcc ttgatgtgat
      361 ccagagcccc ttcttccagg atatcaggga aagacaaccc tacgacgatg acaatctcct
      421 gtgtccctgc atgataatcg accaccccca ggtcctgagg gaagtagtaa ggacccatgg
      481 agcgagggct acccacccgg gatcggagcg tatccttact gaactgtctg agggcctgga
      541 cagctattcc gggaatatcc acgagctgtt cgatcccctc tgggacggag gggggcgtga
      601 gagatacctg aggagccttg agagggaaga caaaccgcgc ccccggggac gtttgaataa
      661 aagattgcaa acagagcaga gaacaggata atctagtcac ggtacaggat acgtaattcc
      721 cgggaggggt gaggagatga agagttacgc ccttgcaata gccatggcga tgctcctggc
      781 ggcagtatat gcttttcaga acaccggtga gatcctggtc aagttccttg tgtgggaact
      841 gtcgttcccg cagggcatat gggaggtgat gctctttgcg gcggggggag tgctgatgtg
      901 gctcgtctcc ctcttcgcca tggtggagag cagatcgaaa tacgtccggc agataaagga
      961 aaaggatgcc aagatcaggg aaatggaagg tgagagatcc tcgctcatgg aagccatcag
     1021 gcacagcggc cctcaatatg aggctgaagc ccagataaga cattccgagg tcacccacga
     1081 agagccggcc gaggaacctg gagtggaaca ggaagggcag tgatagggag aatacccgtc
     1141 tcccggttga tgctccggga ggtggacgat gacgccagga cccttgcaga gagcgtaccc
     1201 tgctcacccc tgacggccct cctgctgaag atgagaggaa tttcagagga tgatccccac
     1261 gaggcccggg cacagctgaa cccgggcctc ggcgattcga tgggccgact ggacctgggc
     1321 ccattctcat ccccggcggg agacctctgg agaagccttg agggagcgga aagggttgtt
     1381 gtttacgggg attacgacgc tgacggagca tgtgcgacag cgctggccat ggaactggcc
     1441 cttgccgcct ttcgtgaggt caggtatttt attccccaca ggcatcgtga gggctacggg
     1501 gtccacgaga aggtcgtccg ggcaattgcc aggaacggtt gcgacctttt gatcgccgtt
     1561 gattgcggga ccagggatat cgcggcactg agggctgccc gggaggcagg catccccgtc
     1621 attgtcttcg atcaccactc cccgggtgag tctctgcccg aaggagccct ggtcgtcaac
     1681 ccccacgccg gaggcggccc tgaggcccag accctctgtg cgacaggtgt cctctgggca
     1741 tgggccagat cggaggggat cgccgccccg gag
//
LOCUS       DAOPKQ010000261         1732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_81668_length_1732_cov_5.269529, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000261 DAOPKQ010000000
VERSION     DAOPKQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81668_length_1732_cov_5.269529"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PK146_07445"
     CDS             <1..397
                     /locus_tag="PK146_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92107.1"
                     /translation="RWGLLNADRLFLMLVRDPESFDLEKAALDMASFSLFLLVNRHVY
                     LPGCFDIDVHQQSAFSWWFWATGTRKEAMDLASRLSQAGRGLIQPAEPADAWTISIED
                     LRVYGNRAESFYEFVDDLAIPVTRGGPGS"
     gene            403..1248
                     /gene="zupT"
                     /locus_tag="PK146_07450"
     CDS             403..1248
                     /gene="zupT"
                     /locus_tag="PK146_07450"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPE92108.1"
                     /translation="MLFAFGLTLFAGLATGVGSAIAFFADRTNYRFLSISTGFSAGVM
                     LYVSFVEILLKGSESLEEAFGASRGEWVNTAFFFAGIALIGVIDYFIPTAENPHEPRS
                     EEETAPLHEDSPKARKVRPPKGSDQEKGLMRTGLFTALAIGIHNFPEGLVTFLAALQD
                     PKLGFAIAVAVALHNIPEGISVSVPIFYATGKRSKAFAYSLLSGMAEPVGALIAYGAL
                     RLFTGGEFSPAVTGALFAMVAGIMVYISLDELLPTSRAFGKGHDSLIGLVTGMAVMAL
                     SLLLM"
     gene            1279..>1732
                     /locus_tag="PK146_07455"
     CDS             1279..>1732
                     /locus_tag="PK146_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPE92109.1"
                     /translation="MTSSSPGRFTPVLVLLLGVIGISTGSIFARFAEASPIVISFYRS
                     GIAAAVMAPLVLARHREELFGLQRKDFLRVILAGVFLALHFSTWITSLFYTSIASSVV
                     IVQTIPVWTAILSPFISGDRVSRQTWAGIILSFVGVVIISAGDFALGGK"
BASE COUNT          323 a          480 c          502 g          427 t
ORIGIN      
        1 ccgctggggt cttcttaatg cggaccgcct ctttctgatg ctggtcaggg acccggagag
       61 ctttgacctc gaaaaggccg ctctggacat ggcgtccttt tcattgttcc tcctcgtcaa
      121 ccgccatgtc tacctccccg gatgttttga tatcgatgtc catcagcaga gcgccttttc
      181 ctggtggttc tgggccaccg ggacccggaa agaggcgatg gacctggcgt caagactgag
      241 tcaggcggga agaggtctga tccagcccgc cgaaccagcg gacgcgtgga ccataagcat
      301 tgaggacctc agggtctacg gcaacagggc cgagtcattc tacgagttcg tggacgatct
      361 ggccattcct gttaccaggg gggggccggg gtcttgaacg aagtgctttt tgccttcgga
      421 ctgaccctct ttgcgggtct cgccacgggc gtcggaagtg ccatagcctt ttttgccgac
      481 aggacaaact accgtttcct ttccatctct accggttttt ccgcgggtgt catgctctat
      541 gtatctttcg tggagatact tctgaagggc tctgagtcac ttgaagaggc ctttggagcc
      601 tccagggggg aatgggtcaa cacggccttt ttttttgccg ggatcgctct tatcggggtt
      661 attgactatt tcattccgac tgcggaaaat ccccacgaac cgaggagcga ggaagagacg
      721 gccccccttc acgaagactc cccgaaagcc agaaaagtgc gacccccgaa gggaagcgac
      781 caggaaaagg gactcatgcg cacgggcctc ttcactgccc tggcgatagg gatccacaac
      841 tttcctgaag ggcttgtgac tttccttgcg gccctccagg atccgaaact tggttttgcc
      901 atagcggtgg cggtcgctct gcacaacata cccgagggca tcagcgtctc tgtcccgatc
      961 ttctacgcca cgggaaaaag atcgaaggca tttgcctact ctctccttag cggtatggct
     1021 gaaccggtag gggctcttat cgcttacggg gcgctgcgtc tgtttaccgg gggagagttc
     1081 tccccagccg ttaccggggc cctttttgcg atggtggccg ggatcatggt atatatcagc
     1141 cttgatgaac tgctcccaac gagccgcgct tttgggaaag gtcacgacag cctgatcggc
     1201 ctggtgacag gcatggcggt aatggcactg agtcttctgc ttatgtagac aaccgaggtc
     1261 aaagaagggg gttcccaggt gacatcatct tcaccgggac gattcacacc ggtccttgta
     1321 ctgcttcttg gggtcatagg gatatcgacg ggttcgatct ttgcgcgttt tgccgaagcc
     1381 tcgcctatag tgatctcctt ctaccgttca gggatagccg ctgccgttat ggccccactt
     1441 gtgcttgccc ggcacaggga agagctcttt ggactgcaga ggaaggattt tctccgggtg
     1501 atccttgccg gggtctttct tgccctgcac ttttcaacgt ggatcacgtc cctcttctat
     1561 acctcgatcg ccagcagcgt ggtgatagta cagaccattc cggtctggac ggctatcctc
     1621 tcccccttta tcagcggcga cagggtcagc aggcagacct gggctggtat tatccttagt
     1681 ttcgtgggtg ttgtcataat aagcgcaggg gatttcgcgc tggggggcaa gg
//
LOCUS       DAOPKQ010000262         1721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_82489_length_1721_cov_3.276110, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000262 DAOPKQ010000000
VERSION     DAOPKQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82489_length_1721_cov_3.276110"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="kdsA"
                     /locus_tag="PK146_07460"
     CDS             <1..721
                     /gene="kdsA"
                     /locus_tag="PK146_07460"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPE92110.1"
                     /translation="VSEICSRLGIQYVFKSSFDKANRTSIHSFRGPGLEKGLEWLSEI
                     RSATGCPVLTDIHLPGQAAIAAEVVDILQIPAFLCRQTDLVVAAAETGKVVNIKKGQF
                     LAPADMGQAVSKCLEAGNDKVVLCERGSSFGYGQLVVDMRSLFIMRSLGYHVMFDATH
                     SVQMPGAQGTSSGGDRRFVPVLARAAAAAGIDALFIETHPDPDSAKSDGPNMVSLDAL
                     EPLLEKILAIHTAASGTGRES"
     gene            734..>1721
                     /locus_tag="PK146_07465"
     CDS             734..>1721
                     /locus_tag="PK146_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPE92111.1"
                     /translation="MPGDSEHAEFSDGSLLETGRNVLSMEAEELQRAASRVGQELVEA
                     ARIIHRCEGRLVVVGMGKSGLVGRKIAATLASLGTPAFFLHAAEGAHGDLGMVCRDDA
                     GLFLSNSGQTREVLEVLPFFRRLGAPVIAMTGNPRSRLARDADIVIDSGVRQEADPLG
                     LAPTTSTTLQLALGDAIAGMVTRLRGLKADDFAMFHPGGSLGKKLLLRVCDVMATGER
                     VPRISDGVTVREALFEITSKGYGATVLEDGEGRISGIFTDGDLRRLLEDRGVEGLSLP
                     VREVMTKNPRTISPDRLAAEAVHIMEEREISCLVVSEEGSAAGILHIHEMLRA"
BASE COUNT          345 a          467 c          579 g          330 t
ORIGIN      
        1 ggtttcggag atatgttcaa ggctgggtat ccagtatgtt ttcaagtcat ccttcgacaa
       61 ggccaaccgg acctctatcc acagtttccg gggtcccggg ctcgaaaaag ggctggagtg
      121 gctttcggag atcaggtccg caacggggtg cccggtactg acggacattc atcttccggg
      181 acaggcggcc atcgctgccg aagttgtgga tatcctccag attccggctt tcctctgcag
      241 gcagacggat ctggtcgtgg cggcggcgga gacaggcaag gttgtaaaca tcaagaaggg
      301 acagttcctg gctcccgcgg acatgggtca ggcggtctcc aagtgtctcg aggctggcaa
      361 cgacaaagta gtcctgtgcg agaggggcag ttccttcggc tacgggcaac tggtggtgga
      421 tatgagatcc ctcttcataa tgcgctccct tggctatcac gtaatgtttg acgcgaccca
      481 cagcgtgcag atgcctggcg cgcaggggac atccagtggt ggcgacaggc gtttcgttcc
      541 cgttctggca cgggcagcgg cagcggccgg tatcgacgcg cttttcatag agactcaccc
      601 cgatcccgat tcagcaaaga gcgacggccc gaacatggtc tccctggacg cgctggagcc
      661 ccttctggaa aaaattctcg ccattcatac ggccgcttcg ggaactggaa gggagagttg
      721 acccctggac aggatgccgg gagatagcga acacgcggag ttcagtgacg gatccctcct
      781 tgagactggc cggaacgtcc tttctatgga ggccgaggaa cttcagcggg cagcatcgcg
      841 agtaggtcag gaactggtgg aggccgcgcg gataatccat cgctgcgagg gcagactcgt
      901 cgtcgtgggg atgggtaaat ccggcctggt ggggcgcaag atagcggcga cgctggcttc
      961 ccttggcact ccggcctttt tcctgcatgc ggcggagggg gcccacggag accttggaat
     1021 ggtctgcagg gatgacgccg ggctcttcct gagcaacagt gggcagacca gggaggtcct
     1081 tgaagtgctc cccttcttcc ggaggcttgg cgccccggtg atagccatga cagggaatcc
     1141 gcgatccagg cttgccaggg acgcggatat cgtcatcgat tccggggtca ggcaggaggc
     1201 ggaccctctg gggcttgccc cgaccaccag cacgaccctg caactggccc ttggagacgc
     1261 tatcgccggc atggtaacca ggctgagggg cctgaaggcc gatgattttg ccatgttcca
     1321 ccccggcggt tccctgggaa agaaactgct cctgagggtc tgcgatgtaa tggccacggg
     1381 ggagagagtt ccccgtatct ccgatggcgt gaccgtcagg gaggctcttt tcgagataac
     1441 cagcaagggt tacggcgcta ccgtgctgga ggatggggaa ggcaggatca gcggcatatt
     1501 taccgacgga gacctgcgga ggctcctgga ggacaggggt gtcgagggtc tatccctgcc
     1561 ggtaagggaa gtaatgacaa aaaacccgcg gacaatatcg ccggatcgcc tcgcggcgga
     1621 ggccgtgcac ataatggagg agagggagat atcctgcctg gtggtctccg aggaaggaag
     1681 cgccgcgggg atactgcaca ttcatgaaat gctaagggcc g
//
LOCUS       DAOPKQ010000263         1684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_85148_length_1684_cov_4.749540, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000263 DAOPKQ010000000
VERSION     DAOPKQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85148_length_1684_cov_4.749540"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="PK146_07470"
     CDS             <1..1348
                     /locus_tag="PK146_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPE92112.1"
                     /translation="GENKILVFDLGGGTFDVSVLDVGIEDGVIEVLATSGDNLLGGDD
                     WDQKIVDLMVSDFRKNEGIDLSRDRMALQRLREAAEKAKIELSTLSETSISLPFITAD
                     SSGPKHLEMSFTRARFEEITRPLLERVVEPTRRALNDSGLTREEINKVLLVGGATRMP
                     MVQKKIMELLGKEPTKGINPDECVAVGAAIQGAILTGEHKDIILVDVTPLSLGIETLG
                     GVFTKIIDRNTAIPVSKSQIFSTASDSQPQVEIHVLQGERAMAGDNVTLGKFFLDGIP
                     PAPRGVPQIEVTFDIDVNGILNVSARDKGTSREQHVTIQSSRLSEAEIEKMRRAAEDN
                     LEIDRKKKELVDARNEADSLIYSTEKMLTDLGEKVTDTERASIQGEIEEVRSALAGED
                     SGEIRKVCEKLTKSLHEMSTRLYGQDAGKPGAGPEQPSESADGETVDAEFEENGKS"
     gene            1362..>1684
                     /locus_tag="PK146_07475"
     CDS             1362..>1684
                     /locus_tag="PK146_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPE92113.1"
                     /translation="MPSEDSRDLYAILGVSREATQEEIKKAYRKLVRKYHPDANPGDA
                     EAAERFKKINLAYEILQDPQKRAAYDQYGTLGDAPPGGAPFGGFGPFGDIFGDECCCH
                     FLAHY"
BASE COUNT          414 a          435 c          534 g          301 t
ORIGIN      
        1 tggcgagaac aagatactcg tcttcgatct tggtggaggg accttcgacg tatcggtcct
       61 tgacgtgggg atcgaggacg gggttatcga ggtcctggcc acttcaggcg ataacctcct
      121 gggtggggac gactgggacc agaaaattgt agacctgatg gtatccgatt tccggaagaa
      181 cgaggggatc gacctctcca gggacaggat ggccctccag cgccttcgcg aggctgcaga
      241 aaaggcaaag atagaactct cgaccttgtc ggagacctcc atatcgctcc ctttcataac
      301 cgccgacagc agcggaccca agcaccttga gatgtccttt accagggcgc gtttcgagga
      361 gataacccgt cctctgcttg agagagtggt ggagcctacc cggagggcgt tgaacgattc
      421 ggggctgacc agggaggaga taaacaaggt ccttctcgtg ggcggtgcca ccaggatgcc
      481 gatggtccag aaaaagatca tggaactcct cgggaaggag cccacaaagg ggatcaaccc
      541 cgacgagtgc gttgccgtag gagcggctat ccagggcgcg attctcaccg gggagcacaa
      601 ggacataatc ctggtggacg taacgcccct gtcgcttggc atagagaccc tgggcggtgt
      661 cttcaccaag atcatagatc gtaatactgc catacccgtg tccaagagcc agatcttttc
      721 gactgcttcg gacagccagc cccaggtgga gatacatgtc ctccaggggg aacgggccat
      781 ggcgggggac aacgtcaccc tgggaaaatt cttcctcgac gggatacccc ctgcccccag
      841 gggggtgcct cagatagagg tcaccttcga tatcgatgtt aacgggatcc tcaacgtgag
      901 cgccagggac aaggggacct ccagggagca gcatgtgact atccagtcct ccaggctctc
      961 cgaggcggag atcgagaaga tgcgccgtgc cgcagaggat aacctggaga tcgacaggaa
     1021 gaagaaggaa ctggttgatg caaggaacga agccgactca ttgatataca gcacggagaa
     1081 gatgctgaca gatctgggcg aaaaggttac tgacacagag agggccagta tccagggaga
     1141 gatcgaggaa gtccgaagtg ccctggcagg ggaggacagc ggagagatcc gcaaggtatg
     1201 tgaaaagctg accaaaagtc tccacgaaat gtccacccgg ctttacggac aagatgccgg
     1261 taagccgggg gcaggtccgg aacagccatc ggagagcgcc gacggagaaa ccgttgatgc
     1321 tgagttcgaa gagaacggga agtcctaggg agtgaaaccc agtgccctct gaggacagcc
     1381 gggacctgta cgcgatcctt ggggtctccc gggaagccac gcaggaggag atcaagaagg
     1441 cctacaggaa actggtaagg aagtatcacc ccgatgccaa tcccggtgac gccgaggctg
     1501 ccgagaggtt caagaagatc aatcttgctt acgagatact tcaggatccc cagaagagag
     1561 cagcctacga ccagtacggg accctggggg atgcccctcc tggaggggct cctttcgggg
     1621 ggttcggacc cttcggggat attttcgggg atgaatgttg ctgtcacttt ttggctcatt
     1681 attg
//
LOCUS       DAOPKQ010000264         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_85725_length_1677_cov_3.488286, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000264 DAOPKQ010000000
VERSION     DAOPKQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85725_length_1677_cov_3.488286"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PK146_07480"
     CDS             <1..331
                     /locus_tag="PK146_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPE92114.1"
                     /translation="ILKTVTPEELSRAIRVVARGGLYLDGEVARSIEDGDSAFETLSA
                     REREVLLHAARGLSSKEIAGVLIISERTVQTHLASIYDKLGARNKTEAMLLALKYGIV
                     ALEELIE"
     gene            328..>1677
                     /locus_tag="PK146_07485"
     CDS             328..>1677
                     /locus_tag="PK146_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPE92115.1"
                     /translation="MRRHLLILLTVAIMIPSLSALLLGGAGLIQHEKTMEMVARSYVV
                     DIAESFASRLDLSWSRTRTFPLADRERFMRLRQLTWDLSIPGWVAVVDVQGNILLSTG
                     DANALPLLWERGIPIGAAIEVESREGEKFTLAAYPAGETGWYVVAAVSWDKLLGPMLR
                     FSRWPILVGMTGLLGLISVLALWKWLVAPLRVLGTEVSTLQLGKEIPEEDDPSAVFEI
                     RRLRQVLHHLARGAVERAELMKRYVGDIVRVQEEERSRIARDIHDGPLQDVTALIHQI
                     RLARMEGTERSGGLVRLENAEEGARHAVNEMRALCDELSPPWLDLGLSQALDELAERL
                     SRHLGVEISVEVHTEEDLPPDVTLAFFRVVQESVHNSVRHGEASRVNVRFLREGGAAV
                     LEVTDNGKGFDPPADFEELRVRGHRGLANMSERMSLIGGKLEVSRSQEGGTLVRCSWS
                     "
BASE COUNT          357 a          448 c          530 g          342 t
ORIGIN      
        1 cattctcaag acagtgactc ctgaggagct ctcccgggcc atcagggtag tcgcccgcgg
       61 aggtctttat cttgatggag aggtggcacg cagcatcgag gacggcgatt cagccttcga
      121 gacactgtcg gcaagggaga gggaggtcct cctccacgct gcccggggtc tctccagcaa
      181 ggagatcgcc ggagttctca taatcagtga gagaactgtc cagacccatc tggcttcgat
      241 ctacgacaag ctaggggcca ggaacaagac agaggcaatg ctactggccc ttaaatacgg
      301 cattgttgca ctggaggagt taattgaatg aggaggcacc ttctgatcct cctcaccgtt
      361 gccataatga tcccctccct ttctgctctt ctcctgggtg gggcaggtct tatccagcac
      421 gaaaagacca tggagatggt ggcacggtca tacgttgtgg acatcgccga gagctttgcg
      481 tccaggcttg acctgagctg gagcagaaca aggacattcc ctctcgctga tcgtgagagg
      541 ttcatgaggc tgaggcagct tacctgggat ctttcgatac caggctgggt ggccgtagtg
      601 gacgttcagg gcaacatcct gttgtccact ggtgacgcca acgccctgcc tctcctttgg
      661 gaaaggggca tccccatagg tgccgccata gaggtagaaa gcagagaggg cgagaagttt
      721 actcttgctg cctatcctgc cggggagacc ggatggtacg tggtggcagc ggtctcatgg
      781 gacaagcttc tcggtccgat gctccgtttc agccgatggc ccattctcgt cggaatgacc
      841 ggtcttttgg gactgatatc cgttctcgcc ctctggaaat ggcttgtggc ccccctgaga
      901 gtcctgggta cagaggtctc taccctccag ctcggcaagg agatccctga agaagacgac
      961 cccagcgcgg ttttcgagat caggaggctc aggcaggttc tccaccatct ggccaggggg
     1021 gccgtggaga gggcggaact tatgaaaaga tatgtcgggg atatagtcag ggtacaggag
     1081 gaagagcggt cccgaatagc cagggatatc catgacggcc ccctgcagga cgtaacagcc
     1141 cttatccacc agataaggct ggcgaggatg gagggaaccg agcgaagtgg tggtcttgtc
     1201 cgcctggaga acgccgagga gggtgcccga catgccgtaa acgagatgag agctctctgc
     1261 gacgaactct cgccgccgtg gctcgacctg ggcctttcac aggccctgga cgaactggca
     1321 gagaggcttt cgaggcacct gggggtggaa atatccgtgg aagtacatac cgaagaggat
     1381 ctgccacccg atgttacgct tgcctttttc agggtggttc aggaatcggt ccacaactct
     1441 gtccgtcacg gggaggcctc aagggtgaac gtcaggttcc tccgggaggg aggggctgct
     1501 gttctcgaag taaccgacaa cggtaaaggg ttcgaccccc ccgccgattt cgaggagctt
     1561 cgcgtcaggg ggcacagggg cctggccaat atgtccgaaa gaatgtccct catcggaggg
     1621 aagcttgaag tctcaagatc acaggaaggc gggacccttg tccggtgcag ctggtcg
//
LOCUS       DAOPKQ010000265         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_86606_length_1666_cov_2.965239, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000265 DAOPKQ010000000
VERSION     DAOPKQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86606_length_1666_cov_2.965239"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PK146_07490"
     CDS             complement(<1..291)
                     /locus_tag="PK146_07490"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPE92116.1"
                     /translation="MFRCPWSESHQLLMDYHDKEWGVPTHDDRVHFEFLVLESFQAGL
                     SWLTVLKKRENFRRAFEDFDPVRIARYRDSDLERLLCDEGIIRNRQKILAAVR"
     gene            complement(369..1565)
                     /locus_tag="PK146_07495"
     CDS             complement(369..1565)
                     /locus_tag="PK146_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPE92117.1"
                     /translation="MVDASELWKEVEEEIERFSKHAIDLSDWLAKNPEVSEQEFEACR
                     KHTTFLESAGYTVETPFMDIETSFFSRKGDPAGPKVCLMFEYDALPGVGHGCGHNVSG
                     AMSGLAAVGLGRVMDRIKGELVLVGTPAEETNGSKVVLAEKGIFDGMDLAMMIHSSDI
                     FTYAGYRSLAMDAIEFRFTGKPAHAAGEPWAGRNALNGVQLLFHAIDMLRQHIIPEAR
                     IHGIISEGGLAPNIVPEHAGARFYFRAPKRPILDQIVSRIYNCALGAAQATETEVTWR
                     NFEYSFDDMLKNDAGEKFVEDVLKDLGVPIVPSLGTKGSSDVGNVSYRCPALQPKLSI
                     VNEEMASHTHEFAEATLSDSAHEALVTGAKVMARTALAVFLDDDLRRRIREDFEKEKQ
                     EAQHYH"
BASE COUNT          330 a          508 c          448 g          380 t
ORIGIN      
        1 cctgacagcg gcgaggatct tctgcctgtt cctgatgatg ccttcatcgc aaaggagcct
       61 ttcaaggtcg ctatccctgt acctggcgat cctgacaggg tcgaagtcct cgaaggccct
      121 tcggaagttc tctcttttct taagtaccgt cagccagctg agccctgcct gaaaggactc
      181 aaggaccagg aactcgaagt ggacccggtc atcatgggtc ggcacccccc actctttgtc
      241 gtggtagtcc ataagaagct ggtgcgactc tgaccatggg caacgaaaca tgtttgggac
      301 ctccctgtcg agaccgggcc ccggtcattc gaccggggcc cggaagtctt ttattaacag
      361 gttcgggatc agtgatagtg ctgcgcttcc tgtttctcct tctcaaaatc ttcacggatc
      421 ctcctgcgga ggtcgtcatc gaggaacact gccagtgccg tcctcgccat caccttggca
      481 cccgtcacca gggcttcatg ggcgctgtcg gaaagggtcg cctcagcgaa ttcgtgggtg
      541 tgggaggcca tttcctcgtt gactatgctc agtttgggct gaagtgccgg gcaccggtag
      601 gatacgttac ccacatcaga agaccccttg gtcccgaggg acggaacgat cggcacaccg
      661 agatccttca gtacatcctc cacaaacttt tcaccggcgt cgttcttgag catgtcatcg
      721 aagctgtact cgaagttcct ccaggtcacc tccgtctccg tcgcctgggc tgcacctagg
      781 gcacagttgt agatccttga gacaatctgg tcgaggatgg gcctcttggg agcccggaag
      841 tagaacctgg caccggcgtg ctccgggact atgttggggg cgagaccccc ctcggatatg
      901 atcccgtgga tcctggcttc ggggataata tgctgccgga gcatgtctat ggcatggaag
      961 agcagctgca ccccgttgag ggcattcctc ccggcccagg gttccccggc cgcgtgagcg
     1021 ggctttccgg tgaagcggaa ctctatagcg tccatggcca ggcttcggta accggcatag
     1081 gtgaagatgt cgctgctatg gatcatcatt gcaaggtcca tgccgtcgaa gatccctttt
     1141 tcagccagta ccaccttgga gccgttggtc tcctcggccg gggtccccac caggaccagt
     1201 tcccccttga tcctgtccat aaccctgccc aggcccacgg ccgcaagacc cgacatggca
     1261 ccggatacgt tgtgaccgca tccgtgaccg acgccgggaa gggcgtcgta ctcgaacatg
     1321 agacagacct tcggacctgc aggatcacct ttcctggaaa agaaggaggt ctcgatatcc
     1381 atgaagggcg tctctacggt gtaacccgca ctctcaagga aggtcgtatg ttttctgcag
     1441 gcctcgaact cctgctcgga gacctccggg ttcttcgcaa gccagtcact gagatctatc
     1501 gcatgcttcg aaaaacgttc tatctcttcc tctacttcct tccagagttc acttgcatca
     1561 accatatcaa ctcaccccct cattctgcca gaaggatctg gccgttggga gaaccgacaa
     1621 tttcaccttc ctcgacggca acgtcaccgt tgaggataac ccattt
//
LOCUS       DAOPKQ010000266         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_86677_length_1665_cov_3.602484, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000266 DAOPKQ010000000
VERSION     DAOPKQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86677_length_1665_cov_3.602484"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PK146_07500"
     CDS             complement(<1..1213)
                     /locus_tag="PK146_07500"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92118.1"
                     /translation="MKVFLEVIVPGPWWHPLTYESERPAERGVRLSVPVAGRTRTGFA
                     TGSVSGTPPAASFRILKAKEIIDASPPLGWELFETALRMGRHFLCGPGEALKVVSPDH
                     ILAGEPTGDLPRSAPPGESFSERQCFLPFFGDRKEEYHRSILSSKAGVLVLFPEREGA
                     SSFWKGLPPEVKREGVLWTSSSGVAARKRWERVRTGEARIVVGSPAAVFAPLPSMDLV
                     IVEDEGNPAYNSERFPYFNFRSVAGSRARLWGAGLIYGGSVPSARSFLKGASWPGSSG
                     TGKYVFVSLRDARRIIVPGVNEPLNVCDTALSRTREIVQEKGTVIWVLDRKGYSSTVI
                     CGECGRLLTCERCGFPIRWDDGRQVFTCTCCGKENPVSDLCPSCGGRSLQGSRPGLEG
                     ARMVAESVLGED"
     gene            complement(1213..>1665)
                     /locus_tag="PK146_07505"
     CDS             complement(1213..>1665)
                     /locus_tag="PK146_07505"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HPE92119.1"
                     /translation="SADDMYEAAMEEAPSHDIIVKAAAVGDFSPSSFSTSKIKRREGK
                     PLVLEMVPNRDIAAALGASRRPGQFIVGFAAETEDPVENAMGKLRKKGLDLIVVNDVS
                     GPEGAFGSDSNSVRIIGCEGVLSSVEGSKEDVAEAIWDVVARRRRERG"
BASE COUNT          351 a          543 c          430 g          341 t
ORIGIN      
        1 gatcttcgcc cagaacactc tccgccacca tccttgcccc ttcaaggccc ggtctggatc
       61 cctggagaga gcgacctcca caggaggggc aaaggtccga aacggggttc tccttaccgc
      121 agcaggtaca ggtgaagacc tgtcggccat catcccatct gatggggaaa ccacaccttt
      181 cacaggtcag gagtctcccg cactctccac atattacggt gctggaatac cccttcctgt
      241 ccaggaccca gatcacggtc cccttctcct gcactatttc cctggtccgg ctcagagccg
      301 tgtcgcatac gttcaggggt tcattcactc cggggacgat aattctccgg gcgtctcgaa
      361 gagatacgaa aacatacttc cccgttccac tggatcccgg ccaggaagct cccttcagga
      421 aactcctcgc cgaggggacg ctccctccgt agatcaggcc tgcgccccac aggcgggccc
      481 tcgaacccgc aacgctccgg aaattgaaat aggggaacct ttcagagttg taggcagggt
      541 taccctcgtc ttcgacgatc acaaggtcca tcgacgggag aggagcaaag accgcagcag
      601 gggacccgac cactatcctg gcctctcccg tcctcaccct ctcccatctc ttccttgcgg
      661 caacaccgga acttgatgtc cacaggaccc cctctctctt gacctccggc ggaagccctt
      721 tccagaagga cgaagcgcct tccctctccg ggaaaagcac caacacacct gccttggaag
      781 aaaggatcga acggtggtat tcttcctttc tgtctccgaa aaaggggaga aaacactgtc
      841 tctctgagaa agactctccg gggggtgctg atcgggggag atcacctgtt ggctctccgg
      901 caaggatatg atccggggag accaccttga gggcctctcc tggtccgcac aggaaatgcc
      961 tgcccatgcg caaggcggtc tcgaacaact cccaacccag agggggggaa gcatcaatga
     1021 tctctttcgc tttcaggatc ctgaaagatg cagccggggg agttcccgat acggacccgg
     1081 ttgcgaaccc tgtcctggtc ctgccggcta caggaacaga aagcctgacg ccccgttcag
     1141 cgggtctttc ggattcatag gtcaggggat gccaccacgg tcctggtact atcacttcca
     1201 ggaacacttt cactaaccgc gttcccggcg ccgtcgggca actacgtccc agatggcttc
     1261 tgctacatct tccttcgaac cctccacgga ggagagcacc ccctcgcagc cgatgatcct
     1321 gacagagttg ctgtccgatc cgaaggctcc ttcgggtccg ctgacatcat ttacaacgat
     1381 aagatcgagc cctttcttcc tgagcttgcc catggcattc tccacaggat cctcggtctc
     1441 ggcagcaaaa ccgacaatga actgccccgg tctcctggag gctcccagtg ccgcggcaat
     1501 atccctgttg gggaccatct cgagaaccag gggcttgcct tcccttcgtt tgatcttgct
     1561 cgtagagaag gacgagggac tgaagtcccc cactgcagct gccttgacga ttatgtcatg
     1621 ggaaggagcc tcctccatag cggcttcata catgtcatcg gcgga
//
LOCUS       DAOPKQ010000267         1646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_88109_length_1646_cov_4.179761, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000267 DAOPKQ010000000
VERSION     DAOPKQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88109_length_1646_cov_4.179761"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1428
                     /locus_tag="PK146_07510"
     CDS             286..1428
                     /locus_tag="PK146_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPE92120.1"
                     /translation="MQDRIREVLERERENILSVYRDLHAIPELSAQEFRTSAYIADYL
                     EKRGFQVQRGVGGTGLVSYLRGEDPGPVLGVRADMDALGHLVNGEIQAIHSCGHDAHS
                     TMGIFTAVVARELGLVRKGALKMLFQPSEEQEETSGARAMIADGVLKDMDMCIGIHLR
                     PIQEARYGQAVSALKHGALSSVTAQIKGVAAHGGRPHLGKNVVDALAAIVNAVNAIWV
                     DPTVPASAKVTALNAGGANYSSIPEKGEMGADLRANTNEVMEELLEKVEHAIRTGAST
                     IGCSAEVRRVPGLPAAVFDRDMIRIADEAIREILGDEGALGERGTTGAEDFHEYVKAK
                     PELRTTYIGLGCDLEPGLHHPEMSFRQDALLDGVAILANMAGRILG"
BASE COUNT          414 a          394 c          520 g          318 t
ORIGIN      
        1 tggctcaagt acaactggaa actggtcact tactggtcca tcataggtgg gatagccgtt
       61 atcgtcgctt cactggttaa gctgggtccg ttctagggtc aacgaagaac ctgcaaggca
      121 tctaactgac agatcttttc aaaggaaccg cagccagacc tcagtctcgt ttcgcgaggt
      181 ctggctgcca tatccatcaa atatggaaaa aagcgggatc cccgggaaaa aggtgcatcc
      241 tgcctgtcaa ggatagatca atagcacgat aaaaggagga tccagatgca agacaggata
      301 agagaagtgc tggaaaggga aagggagaac atactcagtg tctacaggga ccttcatgct
      361 ataccggagt tatccgccca ggaattcaga acatcggcct atatagcaga ttaccttgaa
      421 aagaggggtt tccaggtcca gagaggagtc ggagggacag ggctggtctc ttaccttagg
      481 ggagaggatc ccgggccggt gctgggtgtc agggcggata tggacgccct ggggcacctg
      541 gtgaacggag agatccaggc cattcactcc tgcggccatg atgctcactc gacaatgggt
      601 atctttaccg ctgtggtggc cagggagctc ggtctggtaa ggaaaggagc cttaaagatg
      661 ctcttccagc cctcggagga gcaggaagag accagtgggg cgcgggccat gatagccgat
      721 ggtgtgctca aggatatgga tatgtgcata gggatacacc tcagacccat acaggaagca
      781 cgttatggac aggctgtatc agccctgaag cacggagcgc tttccagcgt gaccgcccag
      841 atcaaggggg tcgccgccca tgggggcagg ccccaccttg ggaagaacgt ggtcgatgct
      901 ctggccgcga tagtcaacgc cgtgaatgcc atatgggtcg acccgacggt gccggcttcg
      961 gccaaggtca cggctttgaa tgccggaggc gcgaactact cctccatacc ggagaagggg
     1021 gagatggggg cggacctgag agccaacacc aatgaggtaa tggaagaatt gctggagaaa
     1081 gtcgaacatg ccataaggac aggagcctcc acgataggat gcagcgccga ggtcagaagg
     1141 gttccgggtc ttcctgcagc cgtattcgac agggacatga tccggatagc cgatgaggcc
     1201 atccgggaaa tccttggaga tgaaggtgct ctcggggaga ggggaaccac cggggctgag
     1261 gattttcatg agtacgtcaa ggctaaacct gaactcagaa ccacctacat cggtctgggc
     1321 tgtgacctgg agcccgggct tcatcacccg gagatgtcct tcaggcagga cgccctgctt
     1381 gatggcgtgg ccattcttgc gaacatggca ggcaggatcc ttggctgaaa aagcataagc
     1441 ctgttcacga tcgatggccg gatgaagaag ggaggtgaaa gcggccagga cgacagcagg
     1501 aaggaccagg caaggagtcc ctttaaggga ccgggatgat ccttttcgtt tcgaagggag
     1561 gttacatgat gtcggttgaa acaggtagtg agaggaaaaa gcgcttcaag gtccctcata
     1621 cttacgtcat cctgttctcg gttgtc
//
LOCUS       DAOPKQ010000268         1599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_91892_length_1599_cov_4.733808, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000268 DAOPKQ010000000
VERSION     DAOPKQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91892_length_1599_cov_4.733808"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK146_07515"
     CDS             <1..194
                     /locus_tag="PK146_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92121.1"
                     /translation="YGIGWVARMASLNSRLSGIMKAKGLFVLEHSRREAIDPAGWKGW
                     DLYSRTYGETLLTFFSKET"
     gene            212..712
                     /gene="coaD"
                     /locus_tag="PK146_07520"
     CDS             212..712
                     /gene="coaD"
                     /locus_tag="PK146_07520"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPE92122.1"
                     /translation="MIRAMYPGSFDPITNGHIYIAERAAAIFDDLTVGVLNNSQKKYT
                     FSKEERETMTREALSHLPNVRVKHFEGLLVDFMRAEKCRVIIRGLRALSDFEYEFQLA
                     LMNRQLAPEIETFFIVTEARYSYLSSRAVKEVFHFGGAIHDMVPPGVFRRLRERFPPL
                     PGLRQA"
     gene            complement(699..>1599)
                     /locus_tag="PK146_07525"
     CDS             complement(699..>1599)
                     /locus_tag="PK146_07525"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPE92123.1"
                     /translation="MEDPEAPLETVAGILLEEPDTFKKVLKSRLDCGFSYAEARTAAI
                     EHLLPGAGKMLSSPNNILAQEYVQRIIERNYPLRITPIKRTGGGHHSGSSGAFAGAGW
                     IRNTVRNEGLSDTVRTVMPPEALSMLGTEISRGRCILSLDVFWRILRTVLMRDGPSRL
                     SRFAEMREGIENLLTASAARAGSFEEFINLCTSRRYPRSKIQRFACHSLMGFDHRANR
                     KAQRTGPPYIRVLGWDIKGQEILKEMRTASRLPVVTRPNGRPGTYQRMIADLEYRATS
                     IWENLVQSPELFSESGNLPVRPV"
BASE COUNT          321 a          446 c          459 g          373 t
ORIGIN      
        1 cctacggcat cggttgggta gcgagaatgg cctcattgaa ctcccgtctc tctggtatca
       61 tgaaagcgaa aggactgttc gtcctcgaac attcccggag ggaagcgatc gatcctgccg
      121 gctggaaggg gtgggacctt tactcccgga cttacggtga gactttgctc acctttttca
      181 gcaaggagac ttaaagggag ggagagacac cttgatcaga gcgatgtatc ccgggtcttt
      241 cgacccaata acgaacgggc acatatatat tgccgaaagg gcagcggcaa tattcgacga
      301 tctcaccgtg ggtgttctca acaacagtca gaagaaatac accttctcca aagaggagag
      361 ggagaccatg accagggagg ctcttagcca ccttcccaac gtaagggtaa aacacttcga
      421 gggtctcctg gtggatttca tgagagccga gaagtgccgt gtgatcatcc ggggtcttag
      481 agccctttcg gatttcgagt acgagttcca gcttgccctc atgaacaggc aacttgcacc
      541 cgagatagag accttcttta tcgtaaccga agcaaggtat tcctatcttt ccagccgcgc
      601 tgtgaaggag gtctttcatt tcggaggggc gatccacgac atggtgcctc ccggagtctt
      661 ccggaggctg agagagagat tcccgcccct gccaggtctc agacaggcct gacggggagg
      721 ttccctgatt cgctgaagag ctcggggctc tggaccaggt tttcccagat cgaagtggcc
      781 ctgtattcca gatcggcgat catccgctgg tatgtgcccg gcctgccgtt gggacgggtg
      841 acgacgggga gccttgaggc tgtcctcatc tccttgagga tctcctgtcc ttttatgtcc
      901 cagccgagaa cccttatgta gggagggccg gttctttgcg ctttcctgtt cgcacgatgg
      961 tcgaacccca tgagcgagtg gcaggcgaac cgctggatct tgctccttgg atacctccgg
     1021 ctggtgcaga ggtttatgaa ctcctcgaaa gagccggccc ttgcggcgct ggccgtcagc
     1081 agattctcga tcccctccct catctccgcg aatcttgaga gcctggaggg tccgtctctc
     1141 atcagcactg tccgcaggat cctccagaac acatccaggg agagtatgca tctgccccgg
     1201 gatatttccg tacccagcat cgaaagagct tcagggggca ttactgtcct taccgtatca
     1261 gagagacctt cgttccttac ggtgttcctg atccagcctg ccccggcaaa ggcccctgaa
     1321 gatcccgagt gatgtccgcc ccctgtcctc ttgatcggag ttatccggag agggtagttg
     1381 cgctcgatga tccgctggac atactcctgg gccaggatat tattggggga tgaaagcatc
     1441 tttccggcac cgggcagcaa gtgctctatg gcggcggtcc ttgcctcggc gtaggagaag
     1501 ccgcagtcga gtcggctttt gaggaccttt ttgaaggtgt ccggttcttc cagcaggatc
     1561 cctgccactg tctccagcgg tgcttcgggg tcttccatg
//
LOCUS       DAOPKQ010000269         1588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_92767_length_1588_cov_5.015656, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000269 DAOPKQ010000000
VERSION     DAOPKQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92767_length_1588_cov_5.015656"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..549
                     /locus_tag="PK146_07530"
     CDS             7..549
                     /locus_tag="PK146_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPE92124.1"
                     /translation="MKKDRGSPFPAWAVLIAFAIFAALSAYLGFEPGTRSASNFLSFV
                     KSMTGLLPPAFLLVGLFNVWVRRETVEKHLGKDSGVRGYFWAIVLAGTMVGGIYVALP
                     LCVVLQRKGASLGVLLTFLNAAAICRVPMTIFEASFLGVGFTVIRFAVSIPLVILTAR
                     AMSSWLGEGFRITGSAGNKS"
     gene            complement(552..980)
                     /locus_tag="PK146_07535"
     CDS             complement(552..980)
                     /locus_tag="PK146_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPE92125.1"
                     /translation="MNVDLIGKKLETLGRCISRLEQRKGAMDPELQDVIALDLEQGLQ
                     ICLDMASTIILSDHSAPSPTSMPERFDILTMKKVLSPELAEGMKRSIELRNIFLNSCR
                     SADRGTVVSMLASSIRSFREFAESVSRHTGGIGASSPCQG"
     gene            complement(1032..>1588)
                     /locus_tag="PK146_07540"
     CDS             complement(1032..>1588)
                     /locus_tag="PK146_07540"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPE92126.1"
                     /translation="LWQEVFSGSLFRVYTSDDVAGVEIGGAVKNVIAIAAGIAASREM
                     GYNTLAALASRGLAEVMRLGARWGAHPLTLAGLAGVGDLMVTCFSSLSRNYKLGKAVG
                     EGMTLRQAMESLGQVAEGAYTVDALVKHGREIGVELPLAEAVHRILYDGVTPECMVES
                     LFGRELKAELPPEMYWGRETDRRV"
BASE COUNT          281 a          496 c          432 g          379 t
ORIGIN      
        1 actaccatga agaaggacag aggatcgcca ttccctgcct gggcagtgct cattgccttc
       61 gcgatcttcg cggccctttc ggcgtatctg ggttttgagc ccggaacgag aagtgcctcc
      121 aactttctct ccttcgtaaa gtcaatgacg ggtcttctcc ctccagcctt tctcctcgta
      181 gggcttttca acgtctgggt gaggagggag accgtggaga agcatctggg aaaggactcc
      241 ggggtgaggg gttacttctg ggccatcgtg cttgcgggga ccatggtagg aggtatttac
      301 gtcgccttgc ccctctgcgt ggtcctccag aggaaggggg cttccctggg ggtcctgctt
      361 acctttctca acgctgcggc gatctgcagg gtccccatga ccatattcga ggcctctttt
      421 ctgggtgttg gcttcaccgt catccggttc gccgtatcta tccccctggt aatcctgacg
      481 gccagggcca tgtcctcctg gcttggagag ggtttcagga tcaccgggtc agcagggaat
      541 aaaagctgat actatccctg gcagggactg gaagccccga tccctcctgt gtgcctggaa
      601 acggattcag cgaactctcg gaaagatctt atgctggagg ccaacatcga gacgacggtc
      661 ccccggtcgg ccgagcggca gctgttgaga aagatgttcc gaagttcgat ggacctcttc
      721 atcccttctg ccagttcggg tgaaagcacc tttttcatag tcaggatgtc gaacctctcc
      781 ggcatggagg ttggcgacgg tgcggaatgg tctgagagga ttatggtcga tgccatgtcg
      841 aggcatatct ggaggccctg ttccaggtca agggctatta cgtcctggag ctcgggatcc
      901 atcgccccct ttcgctgttc aagtctggaa atgcatcttc caagcgtctc cagtttcttc
      961 ccgatcaggt cgacgttcat tgctcctacc tcctgctctc agcttcggcc acagcttcct
     1021 accgctctct ctcataccct cctgtccgtt tcccttcccc agtacatctc aggggggagt
     1081 tccgctttca gttcccttcc gaagagggat tccaccatgc actccggagt gacgccgtcg
     1141 tagaggatgc gatggaccgc ctctgccagt gggagttcga cgccaatctc cctgccatgt
     1201 ttcaccaggg catccacagt ataggccccc tcggccacct gacccagtga ttccatggcc
     1261 tggcgcagtg tcatcccctc tccgacagcc ttcccaagct tgtagttcct tgagaggcta
     1321 ctgaaacagg ttaccatcag gtcgcccact cctgcgagtc ctgccagcgt cagagggtga
     1381 gccccccacc ttgcgcccag ccgcattacc tcggccagtc ccctgctggc aagggctgcc
     1441 agggtgttgt agcccatctc ccttgaggcg gctataccgg ccgcaatggc gatgacattc
     1501 tttacagcgc ctccgatctc cacccccgcg acgtcatcgc tggtgtagac cctgaagaga
     1561 cttcccgaga agacctcctg ccacagct
//
LOCUS       DAOPKQ010000270         1564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_94856_length_1564_cov_4.260437, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000270 DAOPKQ010000000
VERSION     DAOPKQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94856_length_1564_cov_4.260437"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK146_07545"
     CDS             <1..203
                     /locus_tag="PK146_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92127.1"
                     /translation="LAGVPGRSCKAAIKISDSISLEEAEALLEDLFRCEEPTMCPHGR
                     PTFIRLRDRDLSRLFGRGQSNE"
     gene            196..1155
                     /gene="miaA"
                     /locus_tag="PK146_07550"
     CDS             196..1155
                     /gene="miaA"
                     /locus_tag="PK146_07550"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPE92128.1"
                     /translation="MNEGCREILSVIGPTASGKTLLSLELARRLKGEIISVDSRQVYR
                     FMDIGTDKISREIREEIPHHLIDVVNPDEIFSAADFAARAARAVEEIAARGRVPILAG
                     GTPFYYRALFSDIIDFGGQRWDDTRREYEKKWDKGGSDLLYRRLEGLDPVYAGKVHRN
                     DRFRVIRALSIIDRTGKNPTSIRRESQGRGSCLAPLYVGLYPGRAELYRKIGERVREQ
                     FSSGYPEEVRSLLDRGYGPDLPSMKGFGYRELVAFHEGKISIEEAIEGDIRATKAFSR
                     RQMTWFRRFSPGLWYYVTGVPLQDIARKVVDLWEARIRGQQGT"
     gene            1119..>1564
                     /locus_tag="PK146_07555"
     CDS             1119..>1564
                     /locus_tag="PK146_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPE92129.1"
                     /translation="MGSENKRTTGDLELVFAEPKGFCFGVRRAIASLEESLERHGRVY
                     ALGSPIHNPQEVERLLSKGLVIIETPSEMPMGGTVFVRAHGVSDLVMKELHSLNALVV
                     DGTCPFVSKAKEKAALLSREGYTVMILGDEDHPEVKAIRLSSEGPVT"
BASE COUNT          357 a          411 c          490 g          306 t
ORIGIN      
        1 ccctggccgg ggtccccggc aggtcctgca aggcagcgat caagatcagc gacagcatta
       61 gccttgagga ggcagaggca ctgcttgagg acctcttccg ttgtgaggag cccaccatgt
      121 gccctcacgg aagacctact ttcataaggc ttcgtgacag ggatctgtca agacttttcg
      181 gtagaggtca gagcaatgaa tgaggggtgc agggagatcc tctccgtcat tggtccgacg
      241 gcctcgggga agaccctact ctcccttgag ctggcccgaa ggctcaaagg ggagataatc
      301 tccgtggatt cccgccaggt ctatcgtttc atggatatag ggaccgacaa gataagccgg
      361 gagatccggg aggagatccc tcaccacctg atcgatgttg tgaaccccga cgaaatcttc
      421 tcggcagcgg atttcgccgc aagagccgcc agggctgtgg aagagatagc cgccaggggc
      481 agggtcccca ttctggcagg agggacccct ttctattacc gggccctttt ttccgacatc
      541 atcgactttg ggggccagag gtgggacgat accagaagag agtacgagaa gaagtgggac
      601 aagggaggat cggatctcct ttaccggaga ctggagggcc ttgacccggt ctatgccggc
      661 aaggtccatc ggaacgaccg gttcagggtc atcagggccc tttcgataat cgacaggacg
      721 ggcaagaacc ccacttctat ccggagagaa agccagggaa gaggatcctg tcttgcgccc
      781 ctttacgttg gtctctaccc tggaagggcg gagctttacc ggaagatcgg ggagagggtc
      841 agggagcagt tcagctccgg atatcccgag gaggtcagat ctctcctgga ccgcggctac
      901 ggacctgatc tcccttcgat gaaaggtttc ggctacaggg aactggtggc cttccacgag
      961 ggcaagatca gcatcgaaga ggcaatagag ggagatatca gggcgaccaa ggccttttcc
     1021 agaagacaga tgacatggtt cagaaggttt tcgccaggcc tgtggtatta tgtcacaggc
     1081 gttccgcttc aggatatcgc aagaaaagtg gtggatcttt gggaagcgag aataagagga
     1141 caacagggga cctagagctg gtcttcgccg aaccgaaggg tttctgcttc ggtgtgagaa
     1201 gggccattgc ctccctcgag gagtcccttg aaaggcacgg cagggtctat gccctcggga
     1261 gtcctatcca taacccccag gaggtagaaa ggctactttc aaaaggcctg gtgatcattg
     1321 aaactccctc ggagatgccc atggggggga ccgtatttgt ccgcgcccac ggtgtctccg
     1381 acctcgtcat gaaagagctg cactccctga acgccctggt tgtggacggc acatgcccct
     1441 ttgtgagcaa ggcaaaggag aaggccgccc tcctctcaag ggagggttac accgtaatga
     1501 tcctcgggga cgaagatcac ccggaggtga aggccatcag gctgtcctcg gaagggccgg
     1561 tgac
//
LOCUS       DAOPKQ010000271         1562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_95023_length_1562_cov_4.227605, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000271 DAOPKQ010000000
VERSION     DAOPKQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95023_length_1562_cov_4.227605"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK146_07560"
     CDS             <1..215
                     /locus_tag="PK146_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPE92130.1"
                     /translation="YKGASVVVGIKAEKSLYVETMATYSEGDAFDHEAAAGFIRIWGL
                     PVKLWRQVHPEVEAKGPELKIVSGAK"
     gene            complement(231..518)
                     /locus_tag="PK146_07565"
     CDS             complement(231..518)
                     /locus_tag="PK146_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPE92131.1"
                     /translation="MDGHKCVRVIITGIVQGVGFRWRGRQTAMALEVRGWIRNRPDGS
                     VEAVLQGPSDLVDEMLDWISKGPPGAVVRGITTSPCEADPDLSDFLILGSG"
     gene            602..>1562
                     /locus_tag="PK146_07570"
     CDS             602..>1562
                     /locus_tag="PK146_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HPE92132.1"
                     /translation="MKREVRIEEILERSGKKATIRVSGRAFRPSGGGQPGDSGWLEGE
                     GFRADVRDCTAAGNDLILGIVVKEGEIPLGLRVTATVDEERSRRLSRMHTGEHILSRA
                     LENQLPGLYVDKVSVGEKESTVFMTFDGEIGWDDLFRAEETANGVIRADLPVRVERLS
                     RGSEEGFKDVKIKWDRITDKDVSVVSIEGYDRIACSGSHVVSTAEVGGLIVTGFKGST
                     PEWEVRYTLDRQEILERHSRVVRVLSREIGCEEEKLPSVMARLQEERREASRKLDKAR
                     RYIEIPWERLSEEPGRVLFFGIENFPLELASSGAKRKIEEDPGC"
BASE COUNT          382 a          372 c          503 g          305 t
ORIGIN      
        1 tctacaaggg agcctcggtt gttgtcggga tcaaggccga gaaatccctc tatgtggaga
       61 ccatggctac atattcagag ggtgatgcct ttgaccacga ggcagccgca ggtttcataa
      121 ggatatgggg tctcccggtc aagctctgga ggcaggtgca ccccgaagtg gaggccaagg
      181 gccctgaact gaagatcgtg agcggagcaa aatgaaataa ggatggggca ctagcctgat
      241 cccaggatca ggaaatcaga aaggtccggg tccgcttcgc agggagaggt agtgataccc
      301 ctgacgactg ctccgggggg tcccttgctg atccagtcaa gcatttcatc aacaagatct
      361 gaaggacctt gaaggaccgc ctcgacgctt ccgtcggggc ggttccttat ccatccccga
      421 acttcaagag ccatggcggt ttgccttcct ctccaacgaa aacccacccc ctggacgatc
      481 cctgttatta tgaccctgac acacttgtgg ccgtccattc tatgttcacc tccgcgggtg
      541 tgccaagttt atcatcgtcg gcaaaagctt ctgaatatct ctttcgaggg aaggtcggat
      601 catgaagaga gaagtccgga tagaggagat actggaaaga tcggggaaga aggcaacgat
      661 aagggtatcc ggaagggcct tccgcccatc cggcggggga cagcccggtg acagcggctg
      721 gcttgaaggc gagggtttca gggcggatgt cagggattgc accgctgcag ggaatgatct
      781 cattctgggt attgtcgtga aggaaggaga gatacctctg gggctgcggg taacggcgac
      841 tgtcgatgag gagagaagca gacgcctttc cagaatgcac accggtgaac acatactctc
      901 aagggctctg gagaaccagc ttccgggact ctacgtggat aaggtctccg ttggcgagaa
      961 ggagagcacg gtgttcatga cctttgatgg agagatcggg tgggatgacc tcttcagggc
     1021 ggaagagacc gccaacgggg tcatcagggc cgacctgcct gttcgggtgg agaggctttc
     1081 ccgggggagt gaggaaggat tcaaggacgt aaaaataaag tgggacagaa taaccgacaa
     1141 ggatgtgtca gttgtctcca tcgaaggtta cgacagaata gcctgttcgg ggagtcacgt
     1201 cgtttccacc gccgaagtgg gcgggctgat cgttaccgga ttcaagggaa gcacccccga
     1261 atgggaggtc cggtataccc tggacaggca ggagatactg gagagacaca gcagggtagt
     1321 tagggttctt tcaagggaga tcggctgcga agaggagaaa ctcccctcgg taatggcaag
     1381 gcttcaggag gagcgcaggg aggcctcgag gaagcttgac aaggccagaa gatacataga
     1441 gatcccctgg gagcgtctat cagaggaacc tgggagggtc ctcttcttcg gtatcgagaa
     1501 tttccccctg gagctggcct cctcaggagc aaagagaaag attgaggaag acccgggctg
     1561 ta
//
LOCUS       DAOPKQ010000272         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_95687_length_1554_cov_4.625751, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000272 DAOPKQ010000000
VERSION     DAOPKQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95687_length_1554_cov_4.625751"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PK146_07575"
     CDS             complement(<1..527)
                     /locus_tag="PK146_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPE92133.1"
                     /translation="MPRRSSSPSSLTIAYNFLKRYPALVKDEPVADLANERFHFGNIE
                     WKFWTNRRVLLGALALACLNIGANISFGKITGSIAGVETNIDHLAIYSSLADIGSTLF
                     GGSPVEAIISGTATAPRPLLSSVLMMGIMAAILLFKLLPTIGRFVHRSSIAGFLFILG
                     TFVTFATNIQGALAMA"
     gene            complement(445..966)
                     /locus_tag="PK146_07580"
     CDS             complement(445..966)
                     /locus_tag="PK146_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92134.1"
                     /translation="MITDLLTAIAVVLNGIPQGILALSFGFAAFPTSIAFLVGILGSL
                     AFNSVATISFQAETITLAGTMGNDIRERLSLVFWGAVFLLIPSLLGLNEAIVQFIGPV
                     IVTSMMAGVGIMLAYVSVDLLRSERYSGLVSMVTALAVWFVTKDLAKTIIISVILNHS
                     LQLSEALPGPGKG"
BASE COUNT          422 a          372 c          466 g          294 t
ORIGIN      
        1 gccatggcga gggcgccctg gatattggtc gcgaaggtca cgaaggtgcc caaaatgaag
       61 aggaagcctg caatggaaga tcggtgaacg aaccttccaa tggtaggaag cagtttgaaa
      121 aggaggatcg ccgccatgat ccccatcata aggaccgacg aaagaagtgg ccgtggagct
      181 gtggcagtgc cggatattat tgcctccaca ggacttcctc cgaagagggt ggacccgatg
      241 tcggcgaggc tggagtagat ggccaggtgg tcgatgttgg tttccacccc ggcgatgctt
      301 cccgtgatct tgccgaagct tatgtttgca ccaatgttaa ggcaggccag ggcaagggca
      361 ccgagaagga ccctgcggtt ggtccagaac ttccactcga tgttgccgaa atggaaccgt
      421 tcgttcgcca gatccgctac gggttcatcc tttaccaggg ccgggtagcg cttcagaaag
      481 ttgtaggcta tggttaagga tgacggagat gatgatcgtc ttggcaagat ccttggtgac
      541 gaaccaaact gccagggcag taaccatcga cacaaggccg gagtatcttt cagacctcaa
      601 cagatccacc gaaacgtatg ccagcataat gcccacaccg gccatcatgg aggtaacaat
      661 gacggggcct atgaactgga ctatcgcctc gttcaacccc agaagtgagg ggatcagcag
      721 gaaaactgca ccccagaaga caagactcag gcgttccctt atgtcgttgc ccatggtccc
      781 ggcaagggtt atcgtctcgg cctggaaaga gatagtagca acggagttaa aggccagaga
      841 gcccagtatg ccgaccagga aggcaataga cgttggaaag gccgcgaaac cgaaactcag
      901 ggccaggatg ccctggggga taccgttgag cactacagct attgctgtaa gaagatcagt
      961 aatcatggac tcgaacactc cttgtcttac aattacgcct caaacggcgt taatgtgagg
     1021 tatgaaaacg gaagaagtat aacgccgttt tgccagaatg aaaacctctc tttattatct
     1081 caatttacgg atttcctctc gggcccccca caactatccc aactgcacct gaagagcagg
     1141 cgtcaggaag ctggaggcgg gacactggga aggcaagagc aaaagcgtaa acggtgaacg
     1201 gtgaacggga aacccgaggc ggcaggagac gggaggctgg agacagggaa ggcaagagct
     1261 tgtcattctg aggaatgaaa taacgaagaa tctgatttag gtccccggtt tttacaaaga
     1321 aaggttttct ctgcggagcc ccaccctctg cgtccactgt ggtgagaggg gcttagggcc
     1381 agatctccgc cggtaggcgg acgttccgct cctcaggatg acagaatcaa atcaaatgcg
     1441 taaacggtga acggtgaacg gtgaacggga aacccgaggc ggcaggagac gggaggctgg
     1501 agacagggaa ggcaagagct tgtcattctg aggaatgaaa tgacgaagaa tctg
//
LOCUS       DAOPKQ010000273         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_96818_length_1542_cov_4.669805, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000273 DAOPKQ010000000
VERSION     DAOPKQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96818_length_1542_cov_4.669805"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK146_07585"
     CDS             complement(<1..342)
                     /locus_tag="PK146_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPE92135.1"
                     /translation="MFLQTLITGLSIGGIYALMAVGYSLVFSVLNFSNFAHGAVIMLG
                     AYVGLALATKLNFGFGLVLAGSVAGAGIIAVLNEKLAYSSLRRRKAPSLYLMISAMGC
                     AVFLENLVYATI"
     gene            complement(466..>1542)
                     /locus_tag="PK146_07590"
     CDS             complement(466..>1542)
                     /locus_tag="PK146_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPE92136.1"
                     /translation="KIGHTVSLTGGASMWGQSEARALDMLVKKINENGGVLGKKIEFV
                     RYDNRNDAVESVNVARRLVSDGVAAVIGPAQSGNSIATAPVLEKAKIPMVVTTATNPY
                     VTIDQKTEKVRPFAFRPCFIDPFQGTVAARFAITDLGARKGAILYDVGSDYGQWLAKY
                     FEEAFTDKGGTIVAKEAFRTDELDYRAQLGKIKDLAPDVIFIPTSQKEAAMAAKQARD
                     IGITATLLGTDNWGSPDLIDLGGSAIDGGYFVNLTDLADPDIQDFVAEYKAAFGEDPV
                     LPNPVMAQDALIMIVEALKTAGTTDGEKLAEALANLSDIKVTSGVLTISPEDHNPLDK
                     PAVIQQVDVANKTFKFVKKYVSVD"
BASE COUNT          269 a          452 c          478 g          343 t
ORIGIN      
        1 tatggtggcg tagacaaggt tctccaggaa gacggcgcac cccatggcgc tgatcatcag
       61 gtacagcgaa ggcgccttgc gccttctgag gctggagtag gcaagctttt cgttcaggac
      121 ggcgatgatc cccgctccag cgacgctccc cgccaggaca agtccgaagc cgaagtttag
      181 cttggtggcc agggctagtc ccacgtaggc gcccagcatt atcaccgctc cgtgggcgaa
      241 gttgctgaag ttgagcacgc tgaataccag cgaatatccc accgccatca gggcgtatat
      301 cccgccgatg gacagacccg tgataagtgt ttgaaggaac actccctttc acacatcctt
      361 tgggggtgtc ggggcgacat gttcgacccc gttcccggca ggtcccgccc cccgggagac
      421 cttctgtcgg aggacccgcg gggggcagtg ttgcctgtct tcgaactagt ccacagagac
      481 gtacttcttg acgaacttga aggtcttgtt ggccacgtcg acctgctgga taaccgccgg
      541 cttgtccaga ggattgtggt cctcggggct gatggtcagg accccgctgg tgaccttgat
      601 gtcactgaga ttggcgaggg cttcggcaag cttttctccg tcggtggtac ctgccgtctt
      661 aagggcctcc acgatcatga tcagcgcgtc ctgtgccatt accgggttgg ggaggacggg
      721 gtcctcgccg aaggctgcct tgtattcggc gacgaaatcc tggatatccg ggtcggcaag
      781 gtccgtcagg ttgacgaagt agcctccgtc aatagcgctc ccacccaggt cgatgaggtc
      841 cgggctcccc cagttgtcgg tcccgagaag cgttgcagta atgcctatgt ccctggcctg
      901 tttcgcggcc atggcggcct ctttctggct ggtggggatg aagatcacgt cgggtgcaag
      961 atccttgatc tttccaagct gggcacggta gtcgagctcg tcggtgcgga aggcctcctt
     1021 ggccacgatc gtgccgccct tatcggtgaa ggcctcctcg aagtattttg ccagccactg
     1081 gccgtagtcg gagcctacgt catagaggat ggcccccttc ctcgccccca ggtcggtaat
     1141 ggcgaaccgg gccgcgacgg tgccctggaa ggggtctatg aagcaggggc ggaaagcgaa
     1201 gggcctgacc ttttcagtct tctgatcgat agtaacgtag gggttggtgg ccgttgtcac
     1261 caccatgggt atctttgctt tctccagaac cggggcggtg gctatggagt tgccgctctg
     1321 ggcgggacct ataacggccg ctactccgtc ggagaccagc ctccttgcga cgttgaccga
     1381 ttcgacggca tcgtttctgt tgtcatagcg gacgaactcg atcttctttc cgaggacacc
     1441 gccattctcg ttgatcttct tcacgagcat gtccagcgcc cgggcctcgg actgacccca
     1501 catggaggca cctcctgtga gggatacggt gtggccgatc tt
//
LOCUS       DAOPKQ010000274         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_98844_length_1521_cov_5.515007, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000274 DAOPKQ010000000
VERSION     DAOPKQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98844_length_1521_cov_5.515007"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PK146_07595"
     CDS             complement(<1..519)
                     /locus_tag="PK146_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPE92137.1"
                     /translation="MVVCYVDGVFKALHEVSLPLSDLAFQRGVGIFETIRTYDRRPMA
                     LSPHLERLASSAAGCRIALPLPLEDMKGIIRKGIDLVDGEVKIRPFITGGDAIDNELG
                     FTRPRLFIFFEPLCPLPEKDFREGASLHPVDQTRPMANIKSINYLESFLPLAEAPEAL
                     EVLYCPGGEITES"
     gene            649..>1521
                     /gene="buk"
                     /locus_tag="PK146_07600"
     CDS             649..>1521
                     /gene="buk"
                     /locus_tag="PK146_07600"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPE92138.1"
                     /translation="MASEILAINPGSTSTKIAWFSGKERLWQETVNHDPKEISSFSKV
                     ADQYEFRMKTIEDASRTNGSNLDSLDAVVGRGGLLDPIPGGTYRVDEDLINDLRRGKP
                     WEHASNLGGIIADAIARPRDIPAFIVDPVAVDELDDISRLTGLPEFSKRSLGHALNIK
                     ATVRMAAEDLGKPWDEIDVIVAHLGGGITVCAHRQGRMVDLNNANEFGPFSPERAGGL
                     PAGDLARLCFNGRYTMEELRKRLVGNGGLKAYIGTSDMREVSSRVDSGDEKAALVYRA
                     MALQISKEIASFAAT"
BASE COUNT          342 a          413 c          473 g          293 t
ORIGIN      
        1 tgactcggtt atctcccctc cggggcagta gaggacctca agggcttcgg gggcctctgc
       61 gagggggagg aagctctcca ggtagttgat gctcttgatg ttggccattg gccttgtctg
      121 gtctacgggg tgcagggagg ctccttccct gaagtccttt tcgggaagag ggcagagggg
      181 ttcgaagaat atgaaaagac ggggtctggt gaaaccaagc tcattgtcga tggcgtcacc
      241 gccagttatg aagggcctga tcttcacctc tccatcgacg aggtcgatgc cttttcttat
      301 gatgcccttc atgtcctcca ggggcagggg gagggcgatc ctgcagccgg ccgctgacga
      361 ggcaagcctc tcaaggtggg gagaaagggc catcggcctc ctgtcgtagg tcctgatggt
      421 ctcgaagatc cctacgcctc tctggaaagc aaggtcggaa agagggagag atacctcgtg
      481 aagtgccttg aaaacaccgt caacgtaaca gacgaccata agggagcctc cccgggagct
      541 ttgaattttg cgacggagaa cagtatactc catcaggtcg ggtttaagac tggaacaagt
      601 gtataatttc catgaaaagt tacaagattc cttcagggag gcgaagcgat ggcttccgag
      661 atactcgcta tcaatcctgg ctcgacgagc acgaagatag cctggttctc cggcaaggaa
      721 cggctgtggc aggagacggt gaaccacgat ccgaaggaga tctcctcttt ctcaaaagtt
      781 gccgaccagt acgaattcag gatgaaaacc atcgaggatg cctccaggac caacgggagc
      841 aaccttgatt cgctcgatgc ggtcgtcgga aggggtggac ttctggaccc catccccggc
      901 ggaacctaca gggtggacga ggatcttatc aacgatctcc gccgggggaa accctgggag
      961 cacgcctcca acctgggggg gataatagct gatgcgatcg cccggccccg ggacattcct
     1021 gccttcatcg tcgaccccgt agccgtggac gaactggacg acatctcgcg cctgacgggc
     1081 ctgccggagt tctccaagag atctctcggc cacgcgctca acatcaaggc cactgtcagg
     1141 atggctgccg aggacctggg gaagccctgg gacgagatcg acgtcatagt cgctcacctg
     1201 ggtggaggga taaccgtgtg cgcccaccgt caaggaagga tggtggacct caacaacgcc
     1261 aacgagttcg gccccttctc cccggagagg gcagggggtc tccccgcagg ggaccttgca
     1321 cggctctgtt tcaacgggcg ctacaccatg gaggaattga gaaaacggct tgtggggaac
     1381 ggcgggctca aggcctatat cgggaccagc gacatgaggg aggtatcctc cagagtcgat
     1441 tccggcgatg aaaaggcggc cctggtctac agagccatgg cacttcagat atcaaaggag
     1501 atcgcatcct tcgcggcaac c
//
LOCUS       DAOPKQ010000275         1516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_99306_length_1516_cov_3.749487, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000275 DAOPKQ010000000
VERSION     DAOPKQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99306_length_1516_cov_3.749487"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PK146_07605"
     CDS             <1..927
                     /locus_tag="PK146_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPE92139.1"
                     /translation="NTQNYGIIAFEGAYHGMTGASLAVTSDSGHRDGLGPLVPGVQFV
                     PFPYRYRNPFGCPEEMVDKFAADNLERVLDDTHSGYSKPAAVLLEAVQGEGGTIIPEP
                     EFLQRVREITRERGILMICDEIQCGLGRTGKMFAFEHAGIVPDIVTISKALGGAGFPI
                     SAIAYREELNTMPPGKSIGTFRGNMVAFAAGAAGLNWMIENRVPEYAAELGKKAMVRF
                     KEMEKDCKLLGEARGIGLMIALEIVEDKKTRKPAADLTKQLRRLAHQRGVMIEVGGHH
                     NNVARLLPPLVITEDLLMKAADILEEVLKELE"
     gene            complement(992..>1516)
                     /locus_tag="PK146_07610"
     CDS             complement(992..>1516)
                     /locus_tag="PK146_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPE92140.1"
                     /translation="PHTPPVDVSLPSVLFPVPFGEASFVVDVNVARLAGLLRLTGFDC
                     LYDPRLDDAMIAEVAGGERRILLTRDRELLKRNRVLRGRLIRSDRPWEQLAEVIRFFG
                     LRDRIDLFCRCPRCNVPLVNIDKGDVLDQLEPLTRMMYDRFTRCPGCGQVYWSGSHHS
                     RIREKLAAALDDLQ"
BASE COUNT          369 a          401 c          459 g          287 t
ORIGIN      
        1 aacacccaga actacgggat aattgccttt gaaggcgcat accatggcat gaccggagct
       61 tcccttgccg tgaccagcga cagcggtcac agggacggcc tgggtcccct tgttccggga
      121 gtccagttcg tgcctttccc ctacaggtat cgtaacccct tcggttgtcc ggaagagatg
      181 gtcgacaaat ttgctgccga caacctcgag agggtccttg atgacactca ctcgggttat
      241 tcaaaacctg cggcagtgct ccttgaggct gtccagggcg agggggggac gattatcccg
      301 gaacctgaat tcctccagag ggtcagggag atcacccggg agcgaggtat cctgatgatc
      361 tgtgatgaga tccagtgtgg cctcggaaga acgggaaaga tgttcgcctt tgagcacgcc
      421 ggtatcgtcc ccgatatagt taccatcagc aaggctcttg gcggcgcagg gttccccatc
      481 tccgccatag cctacaggga ggagctgaat accatgcctc ccggaaaaag tataggcacc
      541 ttcaggggca acatggtggc cttcgccgcc ggagcggcag gactaaactg gatgatcgag
      601 aacagggtac ccgagtatgc agcggaactg ggtaaaaagg cgatggtccg tttcaaggag
      661 atggagaagg actgcaaact cctgggcgag gccaggggga tcggcctgat gatagccctc
      721 gagatcgtgg aggacaaaaa gaccaggaaa ccagccgcgg atctcacgaa gcagttgcga
      781 agactggccc accagcgcgg cgtaatgatc gaggtcggag ggcaccacaa caacgtagcc
      841 aggctgcttc ctccgcttgt tatcactgag gatcttctga tgaaggcagc ggatatactc
      901 gaggaagtcc tgaaggaact ggaatagatc ctgaagggat caagtagata aggcttcagg
      961 gtgaacagga tccaaaagaa atttcaccac gttattgaag atcatccagt gctgcggcaa
     1021 gtttttcgcg aatgcgcgag tggtgtgaac cggaccagta aacctgcccg cagccagggc
     1081 agcgggtgaa gcggtcgtac atcatgcgtg tcaatggttc cagctgatcg agaacgtccc
     1141 ccttgtcgat attgacgagg gggacgttgc accttgggca acggcagaac aggtcgatac
     1201 gatccctgag accgaagaaa cggatcactt ctgcaagctg ctcccagggc ctgtcggaac
     1261 ggatcaggcg gcccctgagg accctgttgc gtttcagaag ttccctgtcc ctggtgagaa
     1321 ggatccttct ttccccccct gcaacttcag cgatcattgc atcatccagg cgtgggtcat
     1381 agaggcagtc aaatcctgtc agtctgagca gacccgcaag ccgggccaca ttcacgtcca
     1441 ccacaaagga agcctctccg aagggcacag ggaataaaac cgaggggagg gagacatcga
     1501 cagggggcgt atgggg
//
LOCUS       DAOPKQ010000276         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_99404_length_1515_cov_3.778082, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000276 DAOPKQ010000000
VERSION     DAOPKQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99404_length_1515_cov_3.778082"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PK146_07615"
     CDS             <1..425
                     /locus_tag="PK146_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HPE92141.1"
                     /translation="MGLILSGGQWWAVALGLSLGVLPWNYPVAKTFLGDGGSTLLGFL
                     CVSQFTRELPFLLEGKGIVIWLASMLLIGGVPVIDTLFSFARRVIKGSSPFRPDRGHF
                     HHLLSDRGLPLSGVLAILVSIHALSLWMAILLLGRHGT"
     gene            422..>1515
                     /gene="wecB"
                     /locus_tag="PK146_07620"
     CDS             422..>1515
                     /gene="wecB"
                     /locus_tag="PK146_07620"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPE92142.1"
                     /translation="MRKSSRTEQTRGTVCCVIGTRPEAIKMAPVILKLRESASLAPFV
                     LATGQHTRMMRQSLDFFGIEPDVDLALMKESQSLDYITSKVLEEVGRVLDELRPFFVL
                     VHGDTTTTMASSLAAFYRRSRLGHVEAGLRSGDIVRPFPEELNRMISDRVAGWWFPPT
                     EGAEQNLIKEGCDPSRVIRTGNTVIDALLLATSKVASPCCGDLLSIPAKAPVMLLTAH
                     RRESWGEPLRRICNAVSTIMKENPGLWAIVPMHRNPEVRKVFRDILSDESRVILCDPL
                     DYPDFIWAMKRSDLILTDSGGIQEETTILKVPTLVMRELTERPEAVDYGTSLLVGTDP
                     ERILKAARRALEYGLEGLSEGQPGETPPFGS"
BASE COUNT          300 a          424 c          444 g          347 t
ORIGIN      
        1 tgatgggcct catcctttcg ggtgggcagt ggtgggctgt ggctttgggt ctctctcttg
       61 gggtgctccc ctggaactat cctgttgcga aaaccttcct gggggacgga ggcagcactt
      121 tgctgggttt cctctgtgtt tcccagttca ccagggaact cccgttcctt cttgaaggga
      181 agggtattgt gatctggctt gcttccatgc tccttatcgg aggagtcccg gttatcgaca
      241 ctcttttctc ctttgccaga agggtgataa agggatcttc ccccttccgg cccgaccgcg
      301 gtcacttcca tcatctttta tctgacaggg gattgcccct ttctggagtg cttgccatcc
      361 tggtctcgat ccatgccctc tccctctgga tggctatcct tcttctgggg aggcatggga
      421 catgagaaag tcttcccgga cggaacaaac cagggggacc gtctgttgtg tgatcggcac
      481 cagacctgag gcgatcaaga tggccccggt catactgaag ctcagggaga gcgcctctct
      541 tgcccccttt gtcctggcca cggggcagca taccaggatg atgaggcagt ccctcgactt
      601 tttcgggatc gagcccgatg tggacctggc tctcatgaaa gagagccaga gcctcgacta
      661 catcacctca aaggtgctgg aggaggtcgg aagggttctc gatgaactgc ggcccttttt
      721 tgtactggtc cacggcgaca ccaccactac catggcttcc tccctggcgg ctttctacag
      781 acgatcaagg ctcgggcatg ttgaggctgg tctaaggagc ggagatatcg tccgtccctt
      841 ccccgaggag ctgaacagga tgatctccga ccgggtagca ggctggtggt tcccgcccac
      901 cgagggtgca gaacagaacc tgataaagga aggttgtgac ccctccaggg tcatcaggac
      961 aggtaacact gtcatagatg ccctgctcct ggctacctcg aaggtagcca gcccatgctg
     1021 cggtgacctc ttgtcgattc cggcaaaggc gccggtcatg cttctgacag ctcacagaag
     1081 ggagtcctgg ggtgagccgc tcagaaggat ctgtaatgcc gtaagcacta tcatgaaaga
     1141 gaaccccggc ctctgggcga tagtcccgat gcatcgaaac ccggaggtca ggaaggtctt
     1201 tcgtgacatc ctttccgatg agtcaagggt tatcctctgc gaccctctgg attaccctga
     1261 ttttatctgg gctatgaaga ggagcgatct gatccttacc gacagcggag ggatccagga
     1321 ggagaccacc atcctgaaag ttcccaccct ggtcatgaga gagctgacag aaaggcccga
     1381 ggctgtggat tacgggacct ctcttctcgt cgggaccgac cctgagagga tcctgaaagc
     1441 ggcaaggagg gcactggaat atggtctgga aggcctatcc gaagggcagc ccggggagac
     1501 cccacctttc ggatc
//
LOCUS       DAOPKQ010000277         1513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_3247
            NODE_99561_length_1513_cov_3.781893, whole genome shotgun sequence.
ACCESSION   DAOPKQ010000277 DAOPKQ010000000
VERSION     DAOPKQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864982
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,526
            CDSs (total)                      :: 1,513
            Genes (coding)                    :: 1,498
            CDSs (with protein)               :: 1,498
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99561_length_1513_cov_3.781893"
                     /isolate="AMR_MDS_3247"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PK146_07625"
     CDS             complement(<1..265)
                     /locus_tag="PK146_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPE92143.1"
                     /translation="MKGLPRGFKSIGGIEAERIERARQVFFEIFTGYGYRLLLPSSVQ
                     LFGPCWECLSGDVRSRMVSLGTPSGETGCLRPDLTLAAVSYLSS"
     gene            complement(262..>1513)
                     /gene="hisD"
                     /locus_tag="PK146_07630"
     CDS             complement(262..>1513)
                     /gene="hisD"
                     /locus_tag="PK146_07630"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPE92144.1"
                     /translation="KRRTTAVPEATRNITPRVTEIVEAVRRSGEKAVRQFSRTFDGYT
                     GDLRVSREAIASAEAALPPRVREALAQAAMNVSTFHSFQRSMLREEVVEVMKGVFAGI
                     RFEPVSSAAIYVPGGRYPLPSTAIMGVVAAREAGVGRIIAMTPPSGPDGPHEIVLGAL
                     SMLDVKEVWSIGGAQAIAACAFGIEGMERVDVIAGPGNAYVTEAKRLLFGAVGIDGLA
                     GPSEVTVVADGTARADLVAADLIAQSEHDSQARATLLCLDRELALSVLAEVESLLGKL
                     PDPGTARESWTCNGTVTFCSMEEAAAYANSESPEHLVLAVKAPGKALPLFRSFGSAFL
                     GPFTAQAFGDYVAGTNHILPTGGTARFAGGLWTGTFLRPQTFLETDIGGARRLAELGT
                     PISGAEGLPGHTLAMELRIGVDRS"
BASE COUNT          288 a          464 c          454 g          307 t
ORIGIN      
        1 gagagctcag gtatgatacc gctgccaggg tgagatccgg tctcaggcac cccgtctcac
       61 ccgaaggagt ccccagtgag accatcctgc tgcggacatc accagagagg cactcccagc
      121 aggggccgaa gagctggacc gatgaaggga gcagtagacg atatccgtag cctgtaaaga
      181 tttcgaagaa gacctgtctt gccctttcga tcctctcggc ctcgatgccc cctatgcttt
      241 tgaaacccct gggcaagcct ttcatgagcg atcgacccca atgcggagtt ccattgcgag
      301 agtgtggccc ggcaaaccct cggccccgga tatcggggtt ccgagttcgg ccagtctcct
      361 tgcgccacct atgtcggtct caagaaaggt ctggggtctg aggaatgttc ctgtccagag
      421 cccgccggcg aaacgggctg tgccccccgt aggaaggata tggttagtcc cggcgacgta
      481 atcgccgaag gcctgggcag tgaacggtcc aagaaaggca gagccgaagg acctgaatag
      541 cgggagggct tttcctgggg ccttcacggc caggaccagg tgttcgggcg attcggagtt
      601 cgcatatgct gcggcttcct ccatgctgca gaaggtaacg gtgccgttgc aggtccatga
      661 ctccctggcc gttccgggat cgggcaactt tcccaggaga ctttccacct ctgccagtac
      721 ggagagggcc agttcccggt ccaggcacaa aagggtggcc ctggcctggc tgtcgtgctc
      781 ggactgggcg ataaggtctg ccgcgacaag gtcggccctg gcggtaccgt cggcaacgac
      841 ggtgacctcg ctgggtccag ccagtccatc tatccctacg gcaccgaaga gaagtctctt
      901 ggcctcggtt acataggcat tgccggggcc ggcgatcacg tctacccgct ccatgccttc
      961 aataccgaac gcacaggctg ctatcgcctg ggctccaccg atggaccaga cctccttgac
     1021 gtccagcatg gaaagggctc ccagtactat ttcgtggggg ccatccggcc ctgaaggggg
     1081 ggtcatggcg atgatccttc caacaccggc ctccctggca gccactacac ccattatggc
     1141 cgtacttggc agagggtagc gtccaccggg gacatagatg gcagcgctgg agacaggttc
     1201 gaacctgata ccagcaaata cccctttcat gacctctact acttcttccc tgagcattga
     1261 cctctggaag gagtggaagg tgcttacgtt catcgctgcc tgggcgagtg cctcccttac
     1321 ccggggggga agcgccgctt ccgcggaggc gattgcttcc cttgatacgc ggagatcccc
     1381 ggtatacccg tcaaaggtgc gggaaaactg cctaaccgcc ttctctccgg atcgccggac
     1441 cgcctcgacg atctctgtta ctctgggggt aatgttcctg gtcgcttctg ggacagccgt
     1501 tgtccgtctt ttg
//
