LOCUS       DAOPLS010000001        14988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_4181_length_14988_cov_3.975825, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000001 DAOPLS010000000
VERSION     DAOPLS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 14988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14988
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4181_length_14988_cov_3.975825"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PKV52_00005"
     CDS             complement(<1..1232)
                     /locus_tag="PKV52_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98628.1"
                     /translation="MADPDAVKLGYLRQQQVKVGKTTKTVYSNKKQGGGTYAPSSTQA
                     PEAYQAYQASSQAYQASRKARQAGRQLKKAKQNPQGDVDGDGVPNNIDNNNLVKDNLT
                     PESRTINKETKAQAERREGLNARIQAALTAAQASTPLPPTWLDPLSNNTTLPKAKVVD
                     GPGGTKLVYALEKNNKGQSSWVFSKVEVDGKSYTEQVRNPKRIDNPNYNKKLDVDNDG
                     VSGTRKDYTLLSKQKAREDRETEGPVDDTTTGTTVTGGTTTTPGDSSPSSSPNRNRSA
                     ENRRNASDGRNSSRNNENNNNNNNNHQTGGNTLMKDPNGNNLLARFTKVYGEEGKAKL
                     REALDAFKAAGGDFNENYQIGQYGYNVTLKLDGKEVTDPDVIKEKLVAHASASSAMSS
                     QYADPASAQALTRLQLFGV"
     gene            complement(1318..2493)
                     /locus_tag="PKV52_00010"
     CDS             complement(1318..2493)
                     /locus_tag="PKV52_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPD98629.1"
                     /translation="MSLANRNGRTTWVPHSRAKVTKKYGVKDSQYSAGHHTGTDIAIQ
                     GSSGQKIIWAPSVKGKVVRRGYDPNGYGNYVVIKSENNRQWLLAHMRNPGPRVGTVLN
                     QGDKIGNIGATGNVSGPHLHLEEGKKNSPWVYGEVKKPKLLFGSEAPTRTPNGHTLVS
                     LDSQSNTQNKKASDPSVRIHNASKNNAATTGTLKIHHNLNDAIKEQPKISNTITNVQI
                     KEVDISTAAQKSQAIMVADLNKDGVVTKKEDSVLNAADRNESGVVSKKELNRFVAVAD
                     VNKDGIVVRTEAKAFKTVDSNDDHKISKKELKAFKSIAGKDGEISRKEAKQALKAQID
                     SKQDQREHDLAVEYLRRTSKQSGGSQEVGNQAAALVNAQQNKPKKQDNDGPGKKKKK"
     gene            complement(2566..4806)
                     /locus_tag="PKV52_00015"
     CDS             complement(2566..4806)
                     /locus_tag="PKV52_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPD98630.1"
                     /translation="MAKKKKTTKAKRKSKKIDETAPKTVSYFWRQVYAFALIVLAIFI
                     FLGGFNMGGKLPISLFDITAWLVGFVAYIIPFVLIFLAIHKLRHEEFMIPIGKLLASL
                     LFIVSLSGALHVFLSREDANSAAAAGTYGGHVGSIVSGVSLSVLNDVTSFILLLFLAW
                     VGFMLMFGLSPKVLLQKFKNLFIREEADTDLATLKEQPKKLRMNENVPVEHHKDDEQL
                     ASGPRRSTFKNSVEPLTATENHAPLVMAADPNWKVPPLSLLSAKQDKADPGDVEGNAE
                     RIKQALANFGIEVTMEDANISARVTQYTMRAPQGVKMSKIANLADNISYELGAHSVRV
                     EAPIPGKKAVGVEVPNKDSATVTLRSLLKRPEWVAQKSKLAFAVGQDIAGDPVYADLD
                     SMPHLLIAGQTKSGKSVMVNTLLTSLLYTNSPADVKLILVDPKFVEMEPYNDIPHLLT
                     PVINDPEKCVSALKWAVAEMERRYKILKQEKVREIHAYNEKMPNEKMPYIVIVIDELA
                     DLMQMAARDVEALIVRIAQKARAVGIHLVLATQSPRVTVITGLIKANVPARIAFTVSQ
                     EVESRIILDQSGAEKLLGYGDMLFYTTDMPKPIRVQGSLITTEETNKVTDFLREQQEP
                     TYDDEIVSMPVQFNGKGGIVPDVGPSNSEDSEFKDAVRVVVEGGKASTSLLQRRLRIG
                     YGKASRIIDEMEERGIVGPADGSRPREVLIHSVEEAFGSGATDDEDDITHIPVSDDPR
                     DEYLTH"
     gene            complement(4847..5335)
                     /locus_tag="PKV52_00020"
     CDS             complement(4847..5335)
                     /locus_tag="PKV52_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98631.1"
                     /translation="MVIKRKTPRHLQLVHSGEPQANTPYDIATILDIVGTYLNQQEDG
                     FDTSHTIAIHGEVVLDNTDIPVVPLTVELYDTEVQYGPAKPIVLARFIVARLSSDMQP
                     VPDEQDVYELRRYPFKNSYSLYRISEFSTDKGPRQVAHLCDEDQVNTVADLLDHFHEV
                     NE"
     gene            complement(5457..7493)
                     /locus_tag="PKV52_00025"
     CDS             complement(5457..7493)
                     /locus_tag="PKV52_00025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPD98632.1"
                     /translation="MTEQTNKRPASKGQNNKQPNNKINLNAPAVSRGAAIRAQRRAHD
                     DANRVIKQYLPANVEIAEQQTRRANVITDDFDKLKITYLGGQEEIGEKNMQIIEWKNS
                     AIVIDCGNWLGIELPGINYSINDTTYLEKIKHKIKGYIISHGHLDHLGGLKHIVPKFP
                     APIYGSSFTIGVIQKTFEEIPAEEDQSFVPRTIVMNMDNHERLKIGEFFIELVRVTHS
                     VPESSFIVVDTPVGRIINSGDWRLDPEPLDDKPTDVERLKQLGDEGVLLLMSDSTGTA
                     HLGRTPTEDTLQDSFHDIINHAEGRVFVAIFSSNMNRVQMIVNAAVQAGRKVALDGRS
                     MMNYAEIAIRQGILKIPKGTVTTMREIASLQDNKILVICTGGQGEPNAALQRMAEGNH
                     NHITLKPGDSVIVSSTPIPGNEVRYQTIGNELSARGLHLFRHPTHEVDGCGPLHVSGH
                     ARRDEYKELLHIIRPKYFVPIYAGHLHRRYYVDMAVEEGWPRKNTFLVTNGDSLLLTD
                     NSCEEGPKEPHGNILVDQTGAVVSNVVIRDRLLMGQDGLVVVVLTVDKKTSSNLTSPD
                     IISRGFIYMRDNESLMNDLRVELKRAVTQRFKRVELDRFKQEIKEHVTHFLYDRTQRS
                     PIVIPVINVVGGASSKQQNTKPNEQQDPQVIAEDQAKRFAQMRAQLLGQEVKTD"
     gene            complement(7477..8115)
                     /locus_tag="PKV52_00030"
     CDS             complement(7477..8115)
                     /locus_tag="PKV52_00030"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPD98633.1"
                     /translation="MTKQERLDQIKADIVTSNVCPDLASTATNLVFGDGNPNAEIVFI
                     GEAPGKQEDEQSLPFVGASGKFLNEMLGSINMRREEIYITNIVKYRPPHNRDPQPDEK
                     AEFLPFLQRQLAIIQPTLVVTLGRHSMTCFLPTAKISEVHGQAKRIQWGIGAVDGSKK
                     SEKIIILPLYHPAAALYNGSMRTVLQQDFSTIPTILTLIRKGKYRNNYDRTN"
     gene            complement(8217..10331)
                     /gene="pnp"
                     /locus_tag="PKV52_00035"
     CDS             complement(8217..10331)
                     /gene="pnp"
                     /locus_tag="PKV52_00035"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPD98634.1"
                     /translation="MGDIINPYNKPIITVEKDFLGRKLSLEVNRVGFRATASVIVRYG
                     ETVVLGTAMVSPERLSHFDYFPLSINYEEKMYAAGKVSGSRFIKREGRPSDDAILIGR
                     LIDRPIRPLWPKGYRHEVQGVATILSTDPEFRPDMIAMIAMSAAFSLTGAPFDGPVAG
                     LRVTQVDGEYKAFVKGDEYDKGVLDLVVAGTKNGVMMVEAGASEVSEETIADAIEWAF
                     NAIQPAIELQEELVKKVGVREQAFELDLPDETIQKTVNTWVEGKFGQQLRAPYPERND
                     LVYELRAAFHNAMEDEVGEDDYADLKREYDDAFTLALHKDVREGIIKDGVRPDGRGFE
                     DIRPLSSQTGLLPRAHGSSLFTRGVTQALNIVTLAPLSYAQLVDTMEKSGERRYIHHY
                     NAPGWTVGEVQRIGSPGRREIGHGYLAERALTAVLPSEADFPYMIRSVTEIMSQNGST
                     SMAATCSSCLALMDAGVPLKRPVSGIAMGLIMDGDTPIILSDIADAEDFAGDMDFKVT
                     GTSEGITALQMDMKVHGLPVSVLRKALAQGKTGRAHILQHMVSVMPAPSKELSPYAPR
                     VESIKINPDKIRDVIGKGGEMINRIIDETGAEIDIKDDGTVFIASPDGDSIAKTKEWI
                     ARLTEEPEVGKVYKGTVAKVMDFGAFVTILPGIDGMVHVSEMADKRVENPRDIVNEGD
                     EVMVKLMAIDDRGRLSLSMKKA"
     gene            complement(10601..10867)
                     /gene="rpsO"
                     /locus_tag="PKV52_00040"
     CDS             complement(10601..10867)
                     /gene="rpsO"
                     /locus_tag="PKV52_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPD98635.1"
                     /translation="MIATDKKTDIIKAHQKNKTDTGSPEVQVSIMTARISEITEHLKV
                     NKHDYMARRGLLQLVGKRKRLLKYLADTNPDAYLKLVKKLGLRK"
     gene            complement(10942..11634)
                     /gene="truB"
                     /locus_tag="PKV52_00045"
     CDS             complement(10942..11634)
                     /gene="truB"
                     /locus_tag="PKV52_00045"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPD98636.1"
                     /translation="MIEGLLLIDKPAGISSFGVVAKVRGIVRAKTGIKKIKVGHTGTL
                     DPAATGLLILAVGSYTKKVPLLMKQDKTYFVTMKLGQTSSTGDKEGEISAGNAYVPTQ
                     QEVHDVITFFTGSMMQTPPVFSALKLNGRRAYDLARQGKEFTMQPRPVMVHSSELNSY
                     TYPFVTFTTHVGSGTYIRSLVEDIGNKLGTGAYMSDLRRTKIGSYMLEEAVPLGELNT
                     ETIQSYLQIDLK"
     gene            complement(11683..13665)
                     /locus_tag="PKV52_00050"
     CDS             complement(11683..13665)
                     /locus_tag="PKV52_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98637.1"
                     /translation="MDQNAKAQAVERIKEAQNVLITVSTDPSIDQLSAAIGLTLLLNK
                     LDKRATAVFSGEIPSTLEFLRPEDTIEQTTDSLRDFIVSLDKAKADKLRYKVEDEVVR
                     IFITPYKTKLTEEDLIFSQGDFNVDVVLALGVDNREHIDQAIMAHGRILHDATVVSVM
                     AGEATSDVGSINWQDPNASSLCEMLMSISESLKSGTIDNQIATAFLTGIVAMTDRFKN
                     EKTTPKVMTMSAQLMAAGANQQLIAAELTPPEPEEPEEVPEDQLTEPSDDDDESQQEE
                     GVITLHHDEEPTQEEPTVPIEEVEDDRVHIDDEGEFSNAEQLREVVEQVQQNKHATST
                     HEKRIEPLPESDAPDSITVDEVLSPDAPTTENTDAVEPQTPETTTDSQPPIPGQEIQP
                     STDTSDDTEISLPSPVEQVDYPPITGQQAAYLENAPTDANQQDEFVMQTDEELPSIEP
                     AKEGSYSKFLKDKPTIDKPLNATNDTNAAEMADDPLASIPQAGPVAENYDDSPLLSTQ
                     HDAESPRATEVPTTEDTRLSDDGETGTKPNIPLGPNVSQDNTLSEIEANVENFENQVN
                     EVAGASEEDARQAVMEAISQAGPSEYEPPQQNIGAQFVPLDSEVPPSDGGQSQPAPEQ
                     EPTDPPPSVPPPIMPPQFPIPGAEVYEPSNEPPQPR"
     gene            13813..14238
                     /gene="rpsT"
                     /locus_tag="PKV52_00055"
     CDS             13813..14238
                     /gene="rpsT"
                     /locus_tag="PKV52_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPD98638.1"
                     /translation="MPIIKSAKKRVRVSQKATVRNAKTRRTMRETVKSFHKAIAGGKS
                     KEITDAHTQAVSAIDTAAKKNVIHKNKAARKKSQLSAAAKKAGVKPAAAKKPAAKTTV
                     AKKPAKTPVKPARKAAAKKPAAKKPAVTKPKTSKKNSSK"
     gene            complement(14258..>14988)
                     /gene="holA"
                     /locus_tag="PKV52_00060"
     CDS             complement(14258..>14988)
                     /gene="holA"
                     /locus_tag="PKV52_00060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPD98639.1"
                     /translation="AIETLLNRIADTTDVLFIESHLDARSVFAKTLKKQVNEVKHFES
                     IEGPQLQGWVVQEAAKLGGTIAPRDAQLLLDRVGHNQQLIHNELVKLILVDTTISTDL
                     IREMTHVTPSSSVFAMLDAIMQGNVGGASRMYYEQRAQGMEPQAILGMIIWQLHSLAI
                     TVVSRQLPADDIAKKTKLNPFVIRKNMTIAKKISKKQMVALLDAVIATDTTIKTGKAK
                     ADSAIHALFIELASIINPTQSAVS"
BASE COUNT         3636 a         3330 c         3073 g         4949 t
ORIGIN      
        1 acgccgaaca actgtagacg cgtaagagct tgcgcagagg caggatcggc atactgtgac
       61 gacatcgcag agctagcgct cgcgtgcgct acaagctttt ctttaataac atccgggtct
      121 gtaacttctt taccatcaag ctttaaggta acgttatacc catattgacc gatttgataa
      181 ttttcattga aatcaccgcc ggctgcctta aaggcatcaa gtgcctcacg taattttgct
      241 ttaccctctt ctccgtacac tttcgtaaat ctggcaagta aattattgcc atttggatct
      301 ttcatcaaag tattacctcc tgtttgatga ttattattgt tattgttatt gttctcgtta
      361 tttctactag aattacgccc atccgaggcg ttgcgcctgt tttcagcaga acggttacga
      421 tttggagagc ttgatgggct actatcacct ggtgtcgtag ttgtaccgcc tgttaccgtt
      481 gttcctgtgg ttgtatcatc gacaggacct tcagtttccc tatcttctcg ggccttttgt
      541 ttagatagta gtgtatagtc ttttctcgtc cctgaaacac catcgttatc tacatctagt
      601 ttcttattat aatttggatt gtctatacgt ttcggatttc taacttgttc ggtataagat
      661 ttgccatcaa cttcgacttt gctgaacacc caactactct gccctttgtt gtttttttct
      721 aacgcataca ctagtttagt accacctgga ccatctacta ctttagcctt tggtaatgtt
      781 gtgttgttag acaaagggtc aagccaagta ggtggtagtg gtgtacttgc ttgagcagcc
      841 gtaagcgcag cttgaatacg agcattaagc ccctcacgtc gctcggcttg tgctttagtt
      901 tctttgttta ttgttcggct ctctggagtc aaattgtctt taactaaatt attattatca
      961 atattgtttg gcaccccatc accatcaaca tcaccctgtg gattttgttt tgctttttta
     1021 agctgccgcc ctgcttgtcg agcttttcta gaggcctgat atgcctgact agaggcctga
     1081 tatgcctgat atgcctcagg tgcctgagta gacgaaggtg catatgtgcc acccccttgc
     1141 tttttattgc tatagaccgt cttagttgtt tttccaacct taacttgttg ttgtcgtaaa
     1201 taacctagtt ttacagcatc tggatctgcc atgtgtgttt ccttgagtgt taaatttgct
     1261 acattactct taattatata ctacaatgct tatgcctatg taagtataat ttaccagcta
     1321 ctttttcttt ttcttacctg gcccatcgtt atcttgcttt tttggcttgt tttgttgagc
     1381 gttaacaaga gcggcagctt gattaccaac ctcttgtgat ccacctgatt gcttgcttgt
     1441 acgtcttaga tattctaccg ctaaatcgtg ttcacgctgg tcttgtttgc tgtctatctg
     1501 ggcttttaag gcttgtttgg cttctttgcg gctgatttcg ccgtctttgc cagcgattga
     1561 tttaaaggct ttaagttctt ttttgctaat tttgtggtca tcgttgctat caaccgtttt
     1621 aaaggctttt gcttcggtgc gaacaactat gccatcttta ttgacatcag cgacggcaac
     1681 aaatctattc aactcttttt tactcacaac accgctttca ttacgatctg ccgcgttaag
     1741 tacactgtct tctttttttg taacaacacc gtctttatta agatctgcta ccatgatggc
     1801 ttgtgacttt tgagctgctg ttgagatatc aacttctttt atttggacat ttgttattgt
     1861 gttgcttatt tttggttgtt ccttgatggc atcatttaaa ttatgatgta tttttagtgt
     1921 tcccgtcgtg gctgcgttgt tctttgaagc attatgtatt cgaacagagg ggtcgctggc
     1981 ttttttattt tgcgtatttg attgactatc aagtgatact agtgtgtgac catttggagt
     2041 acgagtaggc gcttctgaac caaataatag tttgggtttt tttacttctc catacaccca
     2101 cggtgaattt tttttgccct cttctaaatg aagatgtggt cccgacacat tgcctgttgc
     2161 tccaatatta ccaatcttat caccttgatt taaaactgtg ccaacacgcg gaccaggatt
     2221 tctcatgtgt gctaaaagcc attgcctgtt attttcagac ttaattacga cataattgcc
     2281 atatccattc ggatcataac cacgccgtac caccttaccc tttacagacg gtgcccatat
     2341 gattttttga ccgctactac cttgaatagc tatgtctgtt ccagtatgat gaccagcgct
     2401 atactgacta tctttaactc cgtatttttt tgttacttta gccctggagt gtgggaccca
     2461 agtagtgcgc ccgttacgat tagccaatga cattttgttt ttcctttttt agttttttgt
     2521 gcttatggtt ttattaaata caattttaca gcaattgtaa agaggttagt gggtaagata
     2581 ttcatcacgc gggtcatcag acaccggtat atgcgttata tcgtcttcat catctgttgc
     2641 gccggaacca aatgcctctt ctacgctgtg tattaacact tctcttgggc gtgagccatc
     2701 agctgggcca acaatgcccc gttcttccat ttcgtcaata atacggctgg ctttgccgta
     2761 cccaatacgc aaacgccttt gcaacaagct ggtgctggct ttgccacctt ctacgactac
     2821 acgcacggcg tctttaaatt cgctgtcttc gctattgctc gggccaacat ctggcactat
     2881 gccgcctttg ccattaaact gcacgggcat gctcacgatc tcgtcatcgt aggttggttc
     2941 ttgttgttca cgcaaaaagt cggttacttt gttagtttct tctgtagtaa ttaagctccc
     3001 ctgaacacga attggcttag gcatatcggt ggtgtaaaac aacatatcac cgtaaccaag
     3061 tagcttttct gccccgcttt ggtctaagat aatacgcgat tctacctctt gcgacaccgt
     3121 aaaggcaata cgtgcaggca cgttagcttt aattaacccg gtaataaccg ttacacgtgg
     3181 gctctgtgtg gcaagcacca ggtgaatacc aacagcgcgc gctttttgag caatacgtac
     3241 aatcaatgcc tcaacatcac gagccgccat ttgcataaga tctgcaagtt catctataac
     3301 aatcacaata tatggcattt tctcgttcgg catcttttcg ttataggcat ggatttcacg
     3361 aactttttct tgctttaata tcttatagcg ccgttccatt tctgcgacgg cccacttaag
     3421 tgcactcaca cacttttctg gatcgttaat aactggtgtg agtagatgtg gaatatcgtt
     3481 gtatggctcc atttctacaa actttgggtc aaccaaaata agttttacgt ctgctgggct
     3541 attggtgtag agcaaactgg taagtagcgt gttcaccatg accgatttac ctgattttgt
     3601 ttgcccggca ataagtaggt gcggcataga atctaggtct gcgtagacag gatcgcccgc
     3661 tatatcttgg ccaaccgcaa aggctaattt gcttttttgt gccacccatt cgggtcgttt
     3721 gagtaagctg cgcagtgtaa ctgtggcaga gtctttattt ggtacttcta caccaacagc
     3781 ttttttgcca ggaatcggtg cctcgacacg tacgctatgc gctccaagct catatgaaat
     3841 attatccgcc aggttggcga ttttgctcat ctttacccct tgcggagcac gcatagtgta
     3901 ttgcgtcacc cgagcactaa tgttggcgtc ttccatggtg acttctatgc caaaattagc
     3961 tagtgcctgt ttaatacgtt ctgcgttgcc ttcaacatca cctgggtcag ccttatcttg
     4021 tttagcactt aatagcgaca gtggtggaac cttccagtta ggatctgccg ccataaccaa
     4081 tggtgcatga ttttctgttg ccgtgagcgg ttctactgaa ttcttaaagg tagaacggcg
     4141 cggaccactg gcgagttgtt cgtcgtcttt atggtgctcg acaggtacgt tttcgttcat
     4201 tcgtagcttc ttaggctgtt ctttcagcgt tgctagatcg gtgtctgctt cttcgcggat
     4261 aaagaggttt ttaaactttt gcaataacac ttttggcgaa agcccaaaca taagcataaa
     4321 gcctacccat gccaaaaata atagtaatat aaatgatgtt acatcgttta atacacttag
     4381 cgacacaccg ctgacaatac tgccaacatg cccaccgtat gtgcctgccg cggcagctga
     4441 attagcgtct tctctgctta aaaatacgtg caatgcacca gaaagtgaca cgataaatag
     4501 taaactagcc agaagtttac caattggtat cataaactct tcgtggcgaa gtttgtgaat
     4561 agcgagaaat attaatacaa acggaataat gtaggcaaca aagcccacta accatgcagt
     4621 aatatcaaac aaacttattg gcagtttacc gcccatatta aaaccaccca agaatataaa
     4681 aatagcaagc acaattaagg cgaatgcata tacttggcgc caaaaataac tcaccgtttt
     4741 aggagctgtt tcgtcaatct ttttggattt acgttttgct ttcgtggttt tcttcttttt
     4801 tgccattgct tatactattt taccctattt taatatgtac catgagttat tcatttactt
     4861 catgaaaatg atctagtaaa tctgccacag tatttacttg gtcttcgtcg catagatgag
     4921 ctacttgtct tggaccttta tctgtagaaa attcagatat acgatacaaa ctatacgaat
     4981 tcttaaacgg ataccgccta agttcgtaca cgtcttgctc gtctggtacg ggctgcatat
     5041 cagagctaag tctggcaaca ataaaacgcg ccaacacaat tggtttggct ggtccgtatt
     5101 gtacttctgt gtcgtataac tctaccgtaa gtggtacgac gggtatatct gtgttgtcta
     5161 aaacaacttc accgtgaata gcaatggtat ggcttgtgtc aaaaccgtct tcctgctggt
     5221 ttaagtacgt tccaacaata tctaggatag tcgcaatatc gtagggtgtg ttagcctgtg
     5281 gttcaccaga gtggacgagt tgtaaatgtc tgggggtttt tcttttaatt accatgtagt
     5341 atataatacc acactttact gcttatgtct agtttttagt gcagaattag tagcccgaca
     5401 caagactctc ttgtacaaat gatggttacg tggttcaacc tatctaatgc aaatgtttag
     5461 tctgttttaa cttcttggcc aagcagttga gcacgcatct gagcaaaccg tttggcttga
     5521 tcttcagcaa taacttgagg atcttgttgc tcgtttggtt tggtgttttg ctgcttagaa
     5581 gatgccccgc caaccacatt tatgactgga ataactattg gcgaacgttg cgtgcgatcg
     5641 tataagaagt gtgttacatg ttctttgatt tcttgtttaa acctatcaag ttctacacgt
     5701 ttaaagcgct gcgtaacagc tcgttttagc tcgacacgta aatcgttcat aaggctttcg
     5761 ttatcgcgca tataaatgaa accgcggctt atgatatctg gacttgtaag attactgctt
     5821 gtctttttgt ctactgttaa gacaacgacc actaacccgt cttggcccat aagtaaccta
     5881 tctctaatga caacattaga aacgactgca cctgtttggt ctaccaaaat atttccatgg
     5941 ggttctttag gaccctcttc acatgagttg tctgtgagca ataagctatc gccatttgtc
     6001 accaaaaaag tatttttgcg tggccaacct tcttctacag ccatatctac gtagtagcga
     6061 cggtgtaagt ggccagcgta tataggcaca aaatatttag gccgtataat atgcaacaac
     6121 tctttatatt cgtctctacg ggcgtgaccc gatacgtgca atggcccgca tccatctact
     6181 tcgtgggtgg gatgcctaaa gaggtgtaag ccacgcgccg atagctcatt accaatggtt
     6241 tggtaacgca cttcgttgcc tggaatagga gtagagctta ctatgactga gtcacctggc
     6301 tttaaggtaa tgtggttatg gttaccttct gccatgcgtt gcaaagcagc attcggctcg
     6361 ccctgcccac ctgtacaaat aacgagtatc ttgttatctt gcaagctcgc gatttctcgc
     6421 attgttgtaa cggtgccttt ggggattttt aatatacctt ggcgtatagc aatttctgca
     6481 tagttcatca tgcttctacc gtctagcgca acttttctgc cggcctgtac tgcagcgttt
     6541 acaatcattt gcacacggtt catattgcta gaaaatatcg ctacaaacac cctgccttct
     6601 gcatgattga taatgtcatg aaaactgtct tgaagtgtgt cttcagttgg ggttctgcct
     6661 aaatgagccg ttcccgtgct atcactcata agtaaaagta cgccttcatc accgagctgc
     6721 tttaatcgtt caacatctgt tggtttatcg tctagaggct ctgggtctaa gcgccagtca
     6781 ccactattga tgattctgcc aacaggtgta tctactacaa taaagctcga ttctggcact
     6841 gaatgggtaa cacgtacaag ctcaataaag aattcaccaa ttttaagccg ttcatggttg
     6901 tccatattca taacaatagt tcgcggtaca aaagattggt cttcttctgc cggtatttct
     6961 tcgaatgttt tttgaatcac cccaatggta aagctcgaac catagatagg tgccgggaac
     7021 ttaggcacaa tatgttttaa accacctaaa tggtctaagt gaccgtggct aataatatag
     7081 cctttaatct tgtgcttaat tttttctaga taagttgtgt cattaataga atagttaata
     7141 ccaggcagtt caatgcccaa ccagttgcca caatctataa caattgcact gttcttccat
     7201 tctataattt gcatgttctt ttcgccgatt tcttcttggc cgcccagata ggtaatttta
     7261 agcttatcga aatcatcggt gattacatta gctcttcttg tttgttgctc tgcgatttct
     7321 acgtttgctg gcaaatactg cttaatcaca cgattcgcgt cgtcatgggc acgccgctgt
     7381 gcacgaatag cagcaccacg cgatacggcg ggagcattca aattaatttt attatttggt
     7441 tgtttgttgt tttgcccttt agaagcgggc cgcttattag tttgttctgt cataattgtt
     7501 cctatatttt ccttttctaa ttaatgtgag aattgtaggt attgtactga aatcttgttg
     7561 tagtacagta cgcattgatc cgttatacaa tgctgcagct ggatgatata ggggtaaaat
     7621 aataattttt tcgctttttt tgctaccgtc tactgcccct atgccccatt gtattcgttt
     7681 cgcttggccg tgaacttcac taatcttggc tgttggcaga aaacacgtca ttgaatgacg
     7741 gcctaacgtc acgactaatg taggttgtat tatagcaagt tgacgttgca gaaatggtaa
     7801 aaattctgct ttttcgtcgg gttgtggatc gcgattgtgt ggtggccgat attttacaat
     7861 attggttata tagatttctt ctctgcgcat gttaatgctg cccaacattt cgtttaaaaa
     7921 tttaccacta gcaccaacaa acggtaagct ttgttcgtct tcttgtttgc ctggggcttc
     7981 tccaataaac actatttctg catttgggtt accatcgcca aatacaaggt tcgtggcggt
     8041 tgaggccaaa tctgggcaga cattgctcgt gacaatgtct gcttttattt gatctaaacg
     8101 ttcttgctta gtcatgttgg tctttgcaat tagtagacga agctacgaaa agtcctgtgt
     8161 acaacattat ttatattttc acgtacaaac atactcgctt attgctttat ccctatttag
     8221 gcttttttca tacttaggct caagcgaccg cgatcgtcaa ttgccataag ttttaccatc
     8281 acttcatcgc cttcattcac gatatcgcgc gggttttcta cccttttatc tgccatttca
     8341 ctgacatgca ccataccatc tatgccgggt aaaatagtaa caaatgcgcc aaagtccatt
     8401 accttggcaa cagtaccttt atagaccttg cctacttctg gttcttctgt aaggcgagca
     8461 atccactctt ttgtcttggc tatgctatcg ccatctgggc tcgctataaa taccgtaccg
     8521 tcgtctttga tgtctatttc tgcgccggtt tcatcgataa ttctattaat catttcgccg
     8581 cccttgccaa taacatcacg tattttatct gggttgattt ttatagattc tacccgtggg
     8641 gcatatgggc tgagttcttt gcttggggct ggcatgacac taaccatgtg ctgcaatata
     8701 tgtgccctgc cagtttttcc ctgagcaagt gctttacgta aaacagacac cggtaaacca
     8761 tgcactttca tatccatttg tagagcggtt ataccttcgc tggtacccgt tactttaaag
     8821 tccatatcgc cggcaaaatc ttctgcatca gcaatatcac ttaaaatgat tggggtgtct
     8881 ccgtccataa taagacccat ggcgataccg ctaaccggtc gcttaagtgg aacaccagcg
     8941 tccataagtg ctaaacaact actacatgtt gcagccatac tggttgagcc gttttgactc
     9001 ataatttctg tcaccgatcg tatcatgtaa ggaaaatcag cttcgcttgg taaaacagcg
     9061 gttaaagcac gttctgctaa gtaaccatgg ccaatttcgc ggcgaccagg gctgcctatt
     9121 ctttgcactt cgcctactgt ccagccgggt gcattgtaat ggtgtatgta ccgtcgttcg
     9181 cctgattttt ccattgtgtc tactaattga gcgtagctaa gcggtgcgag cgtgacgatg
     9241 tttaatgctt gagtaacacc gcgggtgaac aaactcgagc cgtgtgctcg tggcaataag
     9301 cctgtttggc tacttaaagg acggatatct tcgaaccctc tgccatctgg gcgcacgccg
     9361 tctttaataa tgccttctct gacatcttta tgtagtgcta aggtaaatgc atcgtcgtat
     9421 tcgcgtttta ggtctgcgta atcatcttca ccaacctcgt cttccattgc gttatgaaaa
     9481 gctgcacgga gttcgtatac aagatcattg cgctctggat atggagcacg tagttgttgg
     9541 ccaaatttac cctctaccca tgtattaacg gttttttgaa tcgtttcatc agggagatca
     9601 agctcaaatg cttgttcgcg aacgcctact tttttaacaa gttcttcttg tagttcaatg
     9661 gcgggttgta tggcgttgaa agcccattca atggcgtcgg caatagtttc ttctgaaact
     9721 tcgctggcac cagcttctac catcatgaca ccattttttg taccggctac aactagatct
     9781 agcactcctt tatcatactc gtcgccctta acaaacgctt tgtattcacc atcaacttga
     9841 gtgactctta aaccagcaac tgggccgtca aacggtgcgc ctgttaggct aaaagcagca
     9901 ctcatggcaa tcatagcaat catatctggt ctaaattctg ggtctgtgct tagtatcgtt
     9961 gcgacacctt gcacttcgtg ccgatagcct tttggccaca gtgggcgaat tgggcggtct
    10021 attaaacggc ctattaaaat agcgtcatcg ctcggacgac cttcgcgctt aataaaacgg
    10081 ctcccactga ctttaccagc tgcatacatt ttttcttcat agttaatgct gagcggaaaa
    10141 taatcaaaat ggctaagacg ctctgggctc accatagctg taccaagtac aacggtttcg
    10201 ccgtagcgta caataacgct cgctgttgcc cgaaatccta ctcggttaac ttctaaactg
    10261 agtttacgtc ctaaaaaatc tttttctacg gttattattg gtttattgta ggggttaatt
    10321 atatctccca ttatgggtat aattcctttc ttaagctgtc aaggcaaaag tagcagggtc
    10381 agatatacca agaacaacct ctatgcgtga catatgttct ttcgtatagc cgctccacca
    10441 ccgtcgcagt gtcatgcttg tattattttt taatgtaaga ggttatcgct agttttttta
    10501 gtggtacact caatcgttac acaagtgaga gttaccaacg tactggttag gcctctagca
    10561 tgtcgcaaga aacgtgtctt gcgctacgcg agtggtcaaa ctattttcgt aagcctagtt
    10621 ttttaactaa ttttagatat gcatcagggt tagtgtcagc aaggtattta agtaatcgtt
    10681 tgcgcttgcc aaccaattgc aagagtccgc gacgagccat ataatcgtgc ttattgacct
    10741 ttaagtgctc cgttatttcg cttatgcgag ctgtcataat actaacttgc acctctggtg
    10801 agcctgtgtc agttttgttc ttctggtgcg ccttaataat atcggttttc ttatctgtag
    10861 caatcaagta gtgttcctct ttcgtctaga ttaaatagtt agtattgatg gcgttagttg
    10921 tattctaaca gatatactat tttatttcaa gtctatttgt aggtagcttt gtatcgtttc
    10981 tgtattcaat tcgcctaagg gtaccgcctc ttctagcatg taggaaccaa tttttgttcg
    11041 ccgtaaatca ctcatgtatg caccggtgcc taatttgttg ccaatatctt ctaccagact
    11101 gcgtatgtac gtaccactac caacatgtgt cgtaaaggtg acgaacgggt atgtatacga
    11161 attaagttca ctactatgca ccataactgg gcgtggttgc atcgtaaact ctttgccttg
    11221 gcgtgccaaa tcgtatgctc ggcggccatt cagttttaag gcactaaaca ctggtggagt
    11281 ttgcatcata ctaccagtaa aaaacgtaat tacatcatgc acttcttgtt gtgttggcac
    11341 atacgcgttc cctgcagaaa tctcaccttc tttatcgcca gtagatgaag tttggccaag
    11401 tttcattgtc acaaaatatg ttttatcttg tttcataagc agaggcactt ttttagtata
    11461 tgagcccact gcaagaatca gtaatcctgt cgccgctggg tctagcgtac cagtgtgacc
    11521 taccttaatt tttttgattc ctgttttagc gcgtacaata ccgcgaactt tcgcaacaac
    11581 cccaaaactg gatattcctg ctggcttatc aattaacaat aacccttcta tcatctgttt
    11641 attgtacccc aagtctgccg tgggagtatg tacattaaaa tgttagcgtg gttgtggtgg
    11701 ttcgttgctt ggttcgtata cttcagcacc aggtattggg aactgtggtg gcattatggg
    11761 tggtggcaca cttggtggtg ggtctgttgg ctcttgctct ggagcgggct gtgattgccc
    11821 accatcactt ggcggcactt ctgaatctag cggtacaaac tgagcaccta tgttttgttg
    11881 aggtggttca tactcagaag ggccagcttg gcttatagcc tccataacag cttggcgagc
    11941 gtcttcttca gacgcaccag ctacttcatt tacctggttt tcaaagtttt ctacattagc
    12001 ttcaatttct gataacgtat tgtcttggct tacatttgga ccaagtggaa tatttggctt
    12061 ggtaccagtt tcgccatcgt cgctcaagcg cgtgtcttct gttgtaggca cctcggtagc
    12121 tctcggtgat tcagcatcgt gttgagtaga tagtagtggc gaatcatcgt agttttctgc
    12181 cacaggacct gcctgtggaa tgctcgcgag cggatcgtct gccatttctg ctgcatttgt
    12241 atcgttggta gcgttcagtg gtttatcaat ggttggttta tcttttaaaa attttgaata
    12301 tgaaccttct tttgcgggtt ctatacttgg taattcttcg tctgtttgca taacaaattc
    12361 atcttgctga ttcgcgtccg tcggggcatt ttctaaataa gcagcttgtt gaccagttat
    12421 tggggggtag tctacttgtt ctacggggct aggcaatgaa atttctgtat cgtctgaagt
    12481 gtctgtactt ggctgaatct cttggcccgg aatcggtggc tgactatctg tggttgtttc
    12541 tggtgtttgt ggttctacag catcggtgtt ttcggtggtc ggagcatcag gagaaagtac
    12601 ttcatcaacc gtaatactat ctggtgcgtc actttctggc aatggttcta tccgtttttc
    12661 gtgggtgctc gtagcatgct tgttttgttg cacttgttct acaacttcac gcaattgttc
    12721 tgcattagaa aattcacctt catcatctat gtgcacccga tcatcctcta cttcttctat
    12781 tggaacggta ggctcttctt gcgtgggctc ttcatcatgg tgtagtgtaa tcacaccttc
    12841 ttcttgctgg gattcatcgt catcatccga aggttccgtc agctgatctt ctggaacttc
    12901 ttctggctct tctggttctg gtggagttaa ctcagctgca ataagttgct ggttagcgcc
    12961 cgcagccatt aattgggcac tcatggtcat tactttaggt gtcgtttttt catttttaaa
    13021 cctatctgtc atagcgacga tacctgtcag aaacgcggtg gctatttgat tgtctatggt
    13081 tccagatttt agagattcac taatactcat taacatttcg cacagactac tcgcatttgg
    13141 gtcctgccaa tttatactcc cgacatcgct cgttgcttcg cctgccatga cacttacaac
    13201 ggtcgcatca tgtagtattc tgccgtgcgc cataattgct tggtctatgt gttccctgtt
    13261 gtctacacca agtgctaaaa cgacgtcaac attgaaatca ccttggctaa aaattaaatc
    13321 ttcttctgtg agtttggttt tatatggtgt gatgaatatt cgcaccactt catcttctac
    13381 tttatatcgt agtttatctg cctttgcttt atctaacgat acgatgaaat cacgcaatga
    13441 atcggttgtt tgctctattg tgtcttctgg tctaagaaac tctaatgtag atggtatttc
    13501 gccactaaat actgccgtgg ctcgtttatc gagtttattc aatagtaacg tcaagccaat
    13561 agcagccgaa agttggtcaa tagaaggatc agtacttacc gtaataagta cattttgcgc
    13621 ttcttttata cgttctaccg cttgtgcttt agcgttttgg tccattgttt ttggttttta
    13681 attagagttt acaatgccag cgtatcacgc cgattatgct tatgcaagca aattattgca
    13741 aatttaccaa ttttacagta tactttgctg ttgaagttta taacgataaa gcaatagtaa
    13801 agaaggagct tcatgccaat tattaaatcc gctaaaaagc gcgtgcgcgt tagccaaaaa
    13861 gcaacagtac gaaatgccaa aacaagaaga acaatgagag aaaccgttaa gtcgtttcat
    13921 aaagcaattg ctggtggtaa gtctaaagaa attacagatg cccacacaca agcagttagc
    13981 gctatagata ctgctgcaaa aaagaatgtt atacacaaga ataaagctgc ccgcaaaaaa
    14041 agccagctca gtgctgctgc taaaaaagct ggggttaagc ctgctgctgc taaaaagcca
    14101 gccgctaaaa ccactgttgc caaaaaacca gctaaaactc cagtaaaacc tgctcgtaaa
    14161 gctgctgcaa aaaagccagc cgctaaaaaa ccggctgtta caaaaccgaa aacatccaaa
    14221 aaaaactctt ctaaatagta ttctgttgta tgctttttta gctaaccgct gattgcgtag
    14281 gattaataat agacgcgagt tctatgaata aagcatggat ggcagagtct gctttagcct
    14341 tgccagtctt tatggttgtg tctgttgcaa tgacagcgtc tagtagagct accatttgtt
    14401 tcttagaaat ttttttagca atagtcatgt tcttccttat aacaaacggg ttaagtttgg
    14461 tttttttggc gatatcatct gcgggcagct gacgtgatac aacagtaata gctaaactat
    14521 gcagctgcca tataatcata cccaaaatgg cttgcggttc catgccttga gcccgctgct
    14581 cataatacat tcgtgatgcg ccaccaacat tcccctgcat aatagcatct agcatcgcaa
    14641 acacactgct acttggtgta acgtgtgtca tttctcgtat aagatcggta ctgattgtag
    14701 tgtctactaa aataagcttg acgagctcat tatgaataag ctgctggtta tggccgactc
    14761 tatcaagcag tagctgtgca tctcgcgggg cgattgtacc gccaagcttc gcagcctctt
    14821 gtaccaccca gccttgtaat tgtggtcctt ctatagattc aaaatgtttt acttcgttta
    14881 cctgcttctt aagtgttttc gcaaatacac tacgagcatc taagtgtgat tctataaaca
    14941 aaacatcagt ggtatcggca atcctgttaa gtagggtttc tatagcga
//
LOCUS       DAOPLS010000002        11656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_5793_length_11656_cov_4.125506, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000002 DAOPLS010000000
VERSION     DAOPLS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_11656_cov_4.125506"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..746
                     /locus_tag="PKV52_00065"
     CDS             63..746
                     /locus_tag="PKV52_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD98640.1"
                     /translation="MRILIVEDEHKIANAIKRGLEQHSYAVDVAYNGDDGASMAMSEP
                     YDLFILDRMLPGDIDGLGIVKQIRTENIQTPILMLTAKSRVLDKAEGLNAGADDYLAK
                     PFAFVELIARVRALLRRPQQTIDTTLIYDNLTLDLQAMQAARAQKPIDLTSKEYALLE
                     YFMRNPDRIVTKDQIIQHVWNYDADILPNTVEVYVGYLRSKIDKPFSSSATLLHTKRG
                     FGYYFGVQQ"
     gene            748..1776
                     /locus_tag="PKV52_00070"
     CDS             748..1776
                     /locus_tag="PKV52_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPD98641.1"
                     /translation="MPFALHKGSIRLAGLYLAIIMSISIFFSVTIYSLSINELGRGLR
                     GPRSVIVRPVGPGFSEEIKNDILRERSVLYTEAKERVLARLIVINIFILIGGGALSYY
                     LAVRTLKPIEEAHEAQNRFTADASHELRTPITAMLTENEVTLMDPKLSLQDAKRQLAS
                     NIEELQRLSALSDGLMRIAGLENRTLQKMDVSLQDIIDNAIHSIQTVASAKFIDIDKH
                     PKKSNVTVHGDSFSLQEVFTILLDNAVKYSNKNTKISVNIQKHQNEVTVDVVDRGMGI
                     PAKDVPYIFDRFYRADSSRTHQSVQGHGLGLSIAKDIITKHGGTISVKSTPKKGSVFS
                     VRLPLVKD"
     gene            1942..2793
                     /gene="atpB"
                     /locus_tag="PKV52_00075"
     CDS             1942..2793
                     /gene="atpB"
                     /locus_tag="PKV52_00075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPD98642.1"
                     /translation="MVGLLTFASEDGVPIAAETIFTIGNFGITNSMILGMLVAVGLII
                     TFVLTAIYTTVRPQSKFAFSMESALEFVTSNLKSTLGSEAKARAFLPLFAALFFFILL
                     NNLIGLFPAMGGTVYVVNDEGIKAALLRPFTTDLNGTLALAIISIGTVQYFAIKQRGG
                     WGHLKHYFSIMQPWWNPMNFFIGIIEILGELIRLLTLALRLFGVIYAGEVLLHVITNL
                     SGNFAPIAVFPVVLMEIFFSVIQAYLFLMLASTYLAIGTSTDGHDDIQPESQSTAHQK
                     SMAATNA"
     gene            2833..3051
                     /locus_tag="PKV52_00080"
     CDS             2833..3051
                     /locus_tag="PKV52_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPD98643.1"
                     /translation="MAEIDPAFIKGLTIALGAIGPAIGLGLVGSAAVRSVGRNPEAQS
                     KILTMALIMAGLVDAIMVFVLLIVFSTN"
     gene            3066..3611
                     /gene="atpF"
                     /locus_tag="PKV52_00085"
     CDS             3066..3611
                     /gene="atpF"
                     /locus_tag="PKV52_00085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPD98644.1"
                     /translation="MILLQFASESSAPPPESVTATFGISWQALLFQAITFLLVVWVLK
                     KFVVGKIYSVIDAREKAINDGLAQAAKAKEELANAESEIDAILNEARTQADVVVHNAK
                     KESAEIIKAAEDKASARADAIMHDAEEKLKLDVAKARQALESETARLISEVSGSILRD
                     KITAEKDQKLIMKELEKRREK"
     gene            3601..3996
                     /locus_tag="PKV52_00090"
     CDS             3601..3996
                     /locus_tag="PKV52_00090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HPD98645.1"
                     /translation="MKSRTKVAHFAAEALLNNTIPRDRLVAMLASWLKDTKNTRQSSY
                     LVQDIARQLAHSGYVFITITTAHPITLQTKSSIVDYIHSYYGANVTVETKEVINPAII
                     GGVQIDTPNGSLDASVKRKLIQIIKGVQR"
     gene            3993..5534
                     /gene="atpA"
                     /locus_tag="PKV52_00095"
     CDS             3993..5534
                     /gene="atpA"
                     /locus_tag="PKV52_00095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPD98646.1"
                     /translation="MSSLNIKELSQELQSAIATLDSDAAEFEQSGVVTRISDGIIWVF
                     GLSNCGFSDVIEIATDNGSVRAFALNLNEDEIGAVLLDTDVDVKAGARVTLTGTTLEV
                     PVGPELIGRVVDPLGRPLDGLGPIKTNRTGRVERTAPGVMDRKGVHEPLATGIVAIDT
                     MVPIGRGQRELIIGDRQTGKTAIAIDTMINQARQKTGVINVYVAVGQKRAKVAALVEK
                     LKQEGVMDQTIVVATSSSDPASLLYLAPYAGTAIAEYFRDNKQHALIIYDDLSKHAVA
                     YRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGSGSLTALPIIETQAGDVS
                     AYIPTNVISITDGQIYLETNLFHQGIRPAISVGLSVSRVGGAAQTKATKSVAGTLRLE
                     LAQFRELASFAQFASDLDEDTKKRIARGRLLTEVLKQKQYNPYSVAEQVIAITAATGG
                     SFDHLLAHEVESALHQLIVTVKKDHKKLIENLNKGTKPEESDVKKINDAAKSVSRGFK
                     KEKAS"
     gene            5538..6383
                     /gene="atpG"
                     /locus_tag="PKV52_00100"
     CDS             5538..6383
                     /gene="atpG"
                     /locus_tag="PKV52_00100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPD98647.1"
                     /translation="MASQQAVKQRIQSVSNTRQITKAMQLVAASKLRKSQEAVIGPKA
                     YIENAKALLATLAGHPGALVNPLFKTPNSKGALTIIVTSDRGLAGAYNANVLKAAAKH
                     LKDHPGMDRVITVGKYASRFISKLKDINSLGAYELDSHNLNSEVVTPVLAQSIDEFVA
                     GNVASVDVVYTKYISTVKHEVIIERVLPIKNITNQVKDRLFEPNAEYVFEVAITRALE
                     AQMLQYLLEARASEQASRMLAMKNATDNAGDLIDDLRLEYNNARQAKITQELAEISGG
                     AEALS"
     gene            6431..7813
                     /gene="atpD"
                     /locus_tag="PKV52_00105"
     CDS             6431..7813
                     /gene="atpD"
                     /locus_tag="PKV52_00105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPD98648.1"
                     /translation="MKVDVALKKGKVVQVVGVVVDAEFAPEDLPKLYDAITITRDTGT
                     IYLEVAQHLSETSVRAIALSSTDGVKRGDEIMATGSPITVPIGDETLGRMFNVIGEAI
                     DKKPQPKTKVTASIHRESPALTEQSGKIEILETGIKVIDLIAPVTKGGKVGLLGGAGV
                     GKTVLITELINNIAKYHSGNSVFAGVGERTREGNDLYHEMDESGVLDKTSLVFGQMNE
                     PPGARLRVALTGLTIAEGFRDQGKDVLLFIDNIFRFTQAGAEVSALLGRLPSAVGYQP
                     NLQQEMGALQERITSTKTGSITSVQAIYVPADDLTDPAPATTFSHLDSTIVLSRALTE
                     LGLYPAVDPLESSSTILDPEIVGEEHYQTAREVQRILQRYKDLQDIIAILGMEELSED
                     DKQTVARARRIQRFLTQPFHVAEVFLNKPGAYVSRDETIAGFKEILSGKHDDKPESAF
                     YLKGNIKEVK"
     gene            7814..8224
                     /gene="atpC"
                     /locus_tag="PKV52_00110"
     CDS             7814..8224
                     /gene="atpC"
                     /locus_tag="PKV52_00110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPD98649.1"
                     /translation="MHVTLVTLSGTRYDDVAKEVRLATPNGAMVVLPYHEPMTAVTTA
                     GAVIIVDKVGEEEVFASYGGLMEVSENSVRLLLDEADHARDLVEEEIKEALESAIQLK
                     AKAKDQTELTEAQRLIDRHEVRLGVAQLRRKTRR"
     gene            8335..9135
                     /locus_tag="PKV52_00115"
     CDS             8335..9135
                     /locus_tag="PKV52_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98650.1"
                     /translation="MDDKETKNIDGIKPPEQAQDGSSETINVNVTDTSDQEESADVAI
                     NSDGDIADSPDSVVTENVSDSVEPEASSSETTETEASTIQSEDSDTTTFSANQPAANE
                     PTQATENQPSVADPGIVPSVQPPANNEQPSTDTTRLKKKNKQLKIWVVVLMLLLVAVG
                     SALVVYFAQQNKSKSDLEAQQQTNASLQQQLNQQQQNSTQQTIDDLTTKLKEEQTKNT
                     ELQATIDSQNKQIQEYQTAIKKMLAACGSACSSIDYPTSTEADTNATN"
     gene            9224..9697
                     /locus_tag="PKV52_00120"
     CDS             9224..9697
                     /locus_tag="PKV52_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98651.1"
                     /translation="MDDIKPPRNFGSGPTPTNYGRTPTQQTGNVPPSGQQYAESHAPD
                     QQSHDQVPAESYKLPQKKTGKKRTIALWVLVVLTLAAGAFAVWEFMQIRDLQTQVDEL
                     KQENARLTEKVYSINYDNRDLRQKLELTTTENDSLKELNDKLLETCGAACSTIIP"
     gene            9754..10704
                     /locus_tag="PKV52_00125"
     CDS             9754..10704
                     /locus_tag="PKV52_00125"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD98652.1"
                     /translation="MQQTEITIPDTYVSRLNINGLRGRVLDIPSKRPSKHGKNILLVY
                     GHHSSIERMYSLASYLSTYGTVTMPDLPGFGGMDSFYIINKKPTLDAMAEYLATFIKL
                     HYRNKPIAICGMSYGFLVVTRMLQKYPHMHKQVNLLVSLVGFSKKNDFRFKPATYNTL
                     LYGSRFMSTLPFSFLAKHLFFTKPLIKASYAITAKSHVKMMDATPEERQKRIDFEVYL
                     WKCNDARTYFATSLCFLTVNLTTQKIPLALHHVSVKQDQYFDKAIVLKNLRRIYSKVS
                     AYTASLPNHAPTVISDEADAALLIPQRLKVLLKQYQKSKV"
     gene            10704..>11656
                     /locus_tag="PKV52_00130"
     CDS             10704..>11656
                     /locus_tag="PKV52_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD98653.1"
                     /translation="MKYMKIGIVCPYNMFQFAGGVQDIVVNLHTYLSAQGHSVKIITP
                     RPRAHQDSVSDHYILVGRSAKMNTPFNTMADIGFEADGDEIQAILDREQFDIIHFHEP
                     WVPVLSRQILSRSKSINVATFHAKLPESLLSKSIISTVVPYTKSILKYIDTYTAVSPA
                     AAEHLQSLSDEHVEIIPNGIRLEQFTHTNHLTRKNKKKRIVYLGRLEKRKGIEHLLSA
                     YASLRESHKDVELIIGGNGVKNNMLQRIVAQYEIPDVTFLGYVTEEDKPYLLASADVF
                     CSPALYGESFGIVLLEAMAVGTPVVAGNNSGYASVMTGKGRL"
BASE COUNT         3771 a         2366 c         2474 g         3045 t
ORIGIN      
        1 cggtgtatgt taataaatcg taaaatcagc taatattcag ttaattgatt acaatagtat
       61 atatgcgaat actgatagtt gaagatgaac ataaaattgc caatgccata aaacgcggac
      121 tcgaacagca tagctatgct gtagatgttg catataatgg cgatgatgga gcttctatgg
      181 ctatgagtga accatacgat ttatttattt tagacagaat gctgcctggc gatatagacg
      241 ggcttggtat cgtaaagcaa ataagaaccg aaaatattca aacaccaata ttaatgctca
      301 cggctaaatc tagagtttta gataaagcag aagggttaaa tgccggtgca gatgattatt
      361 tggccaagcc ttttgcattt gttgaactta tcgcacgagt acgcgcccta ctccgtcgcc
      421 ctcaacaaac cattgataca acattaatat acgataattt gacactagat ttgcaggcga
      481 tgcaagccgc gagagcacaa aaacctatag atttaacaag taaagaatat gccctgcttg
      541 aatattttat gagaaatcca gatagaattg tgacgaaaga tcaaatcata caacatgtat
      601 ggaattatga tgcagacatc ttgccgaata ccgtggaggt gtatgtcggc tatttgcgca
      661 gtaaaataga taaacctttt agtagcagcg caaccctact acatacgaaa cgtggttttg
      721 gctattattt tggggtacaa cagtagtatg ccttttgcac tccataaagg atcaatacgc
      781 ttagctggat tgtaccttgc tattattatg tctattagca tattctttag tgttacaatt
      841 tatagcttgt ctattaatga gctcggtaga gggctgcgtg gaccaagaag tgtgattgta
      901 cgccctgtcg gaccgggatt ttctgaagaa ataaaaaacg acattttacg tgaacgcagt
      961 gtactctata cagaagcaaa agagcgggtt ttggcgcggc ttattgtaat aaatatattt
     1021 attctcatag gtggcggtgc cctaagttac tatttagcag tccgaacgtt aaaacctata
     1081 gaagaagccc acgaagctca aaaccgcttt acagcagatg ccagccatga actcagaaca
     1141 ccgattaccg ctatgctaac agaaaacgaa gttacactaa tggaccccaa gcttagtctg
     1201 caggatgcca aacgtcagct cgcaagcaat atagaagaac tgcaacgatt aagcgccttg
     1261 tctgacgggc taatgcgcat tgctgggctc gaaaacagaa cactccaaaa aatggatgtt
     1321 tcactgcaag atataataga taatgctatt catagtatac aaacggtagc atcagctaag
     1381 tttatagata tcgataaaca tccaaaaaag agtaatgtta cagttcatgg cgactctttc
     1441 agtttacaag aagtgtttac tattttgttg gataatgcgg ttaagtacag caataaaaac
     1501 acaaagattt ctgtgaatat acaaaaacat cagaacgaag ttactgtaga tgtagtagac
     1561 agaggtatgg gaatacctgc aaaagacgtt ccttatatat ttgatcgttt ttaccgcgca
     1621 gattcgtcac gtacgcacca gtcggtgcaa gggcatgggc ttggcttgtc aattgcaaaa
     1681 gacattatta caaaacacgg tgggacgatt tctgttaaga gtactccaaa aaaaggaagc
     1741 gtatttagcg tacggctccc tttggttaaa gactaaacag ccgtaaccta ctgaagttta
     1801 tgagcaaatc gtaagattgt ttcattacaa atcatcataa cagaggttga ttgtacgata
     1861 atttttgcta caatataagt aaatgatact aataaaactg cgctgtaaat catcgcctat
     1921 agaagggtgt gttcggatat aatggtgggt ctgttaacat ttgcgtcaga agatggtgtg
     1981 ccaattgctg ccgaaacgat ttttaccata ggcaactttg gtatcactaa ttcgatgata
     2041 cttggcatgc ttgttgcggt aggtctaatt atcacgtttg tacttacagc catatacaca
     2101 accgtgcgac cacaatctaa atttgccttt tctatggaaa gcgcattaga atttgttacc
     2161 agtaatttaa agagcactct cggtagtgaa gcaaaagcac gcgctttttt gccactgttt
     2221 gctgcattat tcttttttat tctgctaaat aatttaatag gcttgttccc agccatgggc
     2281 ggtactgtat acgtcgtcaa cgatgaaggc attaaagcag cactgttgcg accattcacc
     2341 acagacttaa acggcactct tgctcttgcc attatatcta tagggactgt acagtatttt
     2401 gctatcaaac agcgtggagg ctgggggcac ttaaaacact actttagtat tatgcagccg
     2461 tggtggaatc ccatgaactt ttttattggc attatagaaa tacttggtga gcttatacga
     2521 ttattaacac ttgctcttag gttgtttgga gtaatatatg ctggtgaagt gttgctgcat
     2581 gtcattacta acttaagtgg taattttgcg cccattgcgg tgttccctgt tgtgcttatg
     2641 gaaatattct tttctgttat acaggcctac ttattcttaa tgcttgcctc tacctacctt
     2701 gctatcggca catctacaga tggccatgac gacattcaac cagaatcaca aagcactgct
     2761 caccaaaaaa gtatggctgc taccaatgcg tagtgtgaga taataactag taatataacg
     2821 aggagaaaaa taatggcaga aatagaccca gcatttataa aaggattaac cattgcactc
     2881 ggtgctattg gaccagctat tggtctaggg ctcgttgggt cggcagcggt acgatctgtt
     2941 ggacgcaacc cagaagcgca aagcaaaatt ttaaccatgg cgcttattat ggctggtctt
     3001 gtagacgcaa tcatggtatt cgtactactt atcgtattta gcacaaatta gcagtaggta
     3061 tatacgtgat actattacaa ttcgcatctg aatcgagtgc tccaccgcca gaaagtgtaa
     3121 cagctacatt tggtataagc tggcaagcgt tactgttcca agcaataacc tttttgcttg
     3181 ttgtgtgggt attaaagaaa tttgttgtcg gcaagatata ttcagtcatt gatgcccgcg
     3241 agaaagcgat caatgatggt ttggcacaag cagccaaggc aaaggaagaa ctcgccaacg
     3301 cagaatcaga aatagatgca attcttaatg aggctcgtac ccaagcagat gttgtcgtcc
     3361 ataacgctaa gaaagaatct gccgaaatta ttaaagctgc agaagacaaa gcaagtgcac
     3421 gcgcagacgc aattatgcat gacgctgaag aaaaacttaa actcgatgtt gcgaaagcgc
     3481 gccaagcact agaatcagaa acggctcgtc ttattagcga agtatcagga tctattctgc
     3541 gtgacaaaat cactgcagaa aaagatcaaa aactaatcat gaaagagttg gagaaacgcc
     3601 gtgaaaagta gaacaaaagt tgctcatttt gcggccgaag cgctccttaa caatacgata
     3661 cctcgtgaca gattagttgc aatgctcgct tcttggctaa aagatactaa aaataccaga
     3721 caatcgagct atttagtaca agatatcgca cgtcagcttg ctcattcagg ttatgttttt
     3781 attacaatta caactgccca ccccatcaca cttcagacga agagtagcat cgtagactac
     3841 atccattcgt attatggagc gaatgttacc gtagaaacaa aagaagtcat caaccctgcg
     3901 attattggag gagtacaaat agatactccc aatggatcac ttgatgcatc cgttaaacgt
     3961 aaattgatac agattattaa aggagtacaa agatgagttc actcaatata aaagagctga
     4021 gccaagaact gcaatcggcg attgctacat tagatagcga tgcggctgag tttgagcagt
     4081 ctggtgtagt tacacgcata agtgatggta ttatttgggt ttttggtctg agtaattgtg
     4141 gctttagcga tgtcatagaa atcgcaacag ataatggaag tgttcgggcg tttgcgctaa
     4201 acctaaatga agacgaaatc ggtgccgtac ttttagatac agatgttgat gtaaaagctg
     4261 gtgcacgcgt tacgcttacg ggcacaacac ttgaagtgcc tgttggtcca gagcttatag
     4321 gacgagtggt agacccgctg ggaaggcccc ttgatggcct aggacctata aaaacaaacc
     4381 gcacagggag agtcgaacga acagcacctg gcgtaatgga ccgtaaaggt gtccatgaac
     4441 cacttgccac aggtatcgtg gccattgaca cgatggtacc aattggtcgt ggtcagcgcg
     4501 agctcattat tggtgaccga caaaccggca aaacagccat agcaattgac acaatgataa
     4561 accaagcccg ccagaaaact ggggttatta acgtatacgt ggcagtaggt caaaaacgtg
     4621 cgaaagttgc ggctttggta gaaaagctaa aacaagaggg cgtcatggat caaaccattg
     4681 ttgtcgccac aagttcgtct gacccagcta gcctgttgta tttggctccg tacgccggca
     4741 cagctattgc agaatatttt agggacaata aacagcatgc cctgattatt tatgatgatt
     4801 tatcaaagca tgcggttgcg tatcgtcaga tgtccttgtt attacgccgt ccaccaggcc
     4861 gagaagccta cccgggtgat gtcttttatt tgcactcgag attacttgaa cgagctgcca
     4921 aattatctga tgatcttggt tcgggtagct tgacggcatt acctatcatt gaaacgcaag
     4981 ccggtgatgt gtctgcttat attccaacta atgtcatatc aattaccgat ggccaaatct
     5041 acctagaaac taacctattt caccaaggta ttcgccctgc tatttcggtt ggactaagcg
     5101 tgagccgcgt aggtggtgct gcccaaacaa aagcaaccaa aagcgttgct ggtaccttgc
     5161 gacttgaact ggctcagttt agagaattgg catcgtttgc acaatttgcc agcgatttag
     5221 acgaagacac caaaaagcgc atcgcacgag gtcgcttact tactgaagtt cttaagcaaa
     5281 aacaatataa cccatatagt gttgccgaac aggttattgc tattacagca gccactggcg
     5341 gatcttttga ccatttatta gctcacgaag ttgaatctgc tctacaccaa ctaattgtga
     5401 ctgttaaaaa agatcacaaa aaacttatcg agaatcttaa taaaggcact aaaccagaag
     5461 aatctgatgt taaaaagatt aacgatgccg ctaaatcggt aagccgcggg tttaaaaagg
     5521 aaaaagcgtc gtaaactatg gcttcccagc aagcagtaaa acaacgaatc caatctgtat
     5581 ctaacacgag gcagattacc aaagctatgc aactcgttgc tgcaagcaag ctcagaaaat
     5641 cccaagaagc agtcattggg ccaaaagcgt atatagaaaa cgccaaagca ttgttagcca
     5701 cactcgctgg gcatccgggt gcgcttgtga accctttgtt caaaacgcca aattcaaaag
     5761 gcgccttaac aataattgta acaagtgacc gcggccttgc aggagcctat aacgcaaatg
     5821 tcttaaaagc agctgccaag catcttaaag atcacccagg tatggataga gttattactg
     5881 tcggtaagta tgcctcgcgc tttatatcta aattaaaaga tattaattca ctcggtgcat
     5941 atgaactgga tagtcataac ctaaatagcg aggtggtaac accagtcctt gctcaatcta
     6001 tagatgaatt tgtggctggt aacgttgctt ctgtggatgt tgtctataca aagtacatat
     6061 caacggtaaa acacgaagtc atcatcgaac gtgtcttacc aataaaaaat attacgaacc
     6121 aggtaaaaga tagattattt gaaccgaatg ctgaatatgt ctttgaagtc gccatcacca
     6181 gagcgctcga agctcaaatg ctccaatatt tactcgaagc acgagccagt gagcaagcat
     6241 cacgcatgct cgccatgaaa aatgcaacag ataatgcagg tgatcttatt gatgatttgc
     6301 ggctagagta taacaatgct cgccaagcaa agatcaccca agaactcgct gaaattagtg
     6361 gcggagcaga ggcattgtca taaaatgaga ataaccacga taagaattat aagaaagcaa
     6421 tggaggcaat atgaaggttg atgtagcact aaaaaaagga aaggttgtcc aagtagttgg
     6481 tgtcgttgtg gacgccgaat ttgcacctga agatctccct aaactatatg atgcgataac
     6541 tattacacgt gatacaggca caatatacct agaagtagcc caacatctca gtgaaacgag
     6601 tgtccgtgct attgcactta gtagcaccga cggagtcaaa agaggtgatg aaatcatggc
     6661 gacaggtagc cctattaccg tgccaattgg tgatgaaaca cttggacgta tgtttaatgt
     6721 tattggcgaa gcaatcgata aaaagcccca accaaaaaca aaagtaaccg ccagtattca
     6781 ccgtgaatca cctgccctta ctgagcagag tggaaaaatt gaaattctag aaacaggtat
     6841 taaagttata gatttaattg cacctgttac caagggtggt aaggttggtt tgcttggtgg
     6901 tgctggtgtt ggtaaaaccg tgctgataac tgagcttatt aataacattg ctaaatatca
     6961 tagtggtaac tctgtatttg ctggtgttgg cgaacgtacc cgagaaggca acgacctata
     7021 ccacgaaatg gatgaaagcg gcgtgctgga caaaacgagc cttgtgtttg gacaaatgaa
     7081 tgaaccacct ggtgcacgtt tacgtgttgc cttgacaggc ctcaccatag ccgaaggatt
     7141 tcgcgaccaa ggtaaagatg tcttactttt tattgataat atctttcgtt ttacacaagc
     7201 aggcgcagaa gtgtctgcat tgcttggccg gttgccgagc gctgttggct accagccaaa
     7261 cttacaacaa gaaatggggg ccttgcaaga gcgtattaca agtacaaaaa ctggttctat
     7321 tacaagtgta caggctattt atgtaccggc tgacgactta actgacccgg ctccagctac
     7381 cacctttagc catctagaca gcacaattgt actttcacgt gctttgacag aacttggtct
     7441 ataccctgct gttgacccac tagaaagtag ttcaaccatt ttagacccag aaatagttgg
     7501 cgaggaacat taccagacag cacgtgaagt gcaacgcata ttgcagcggt acaaagattt
     7561 acaagatatc attgctatct tgggaatgga agaactatct gaagacgaca aacaaactgt
     7621 cgcgcgcgcg cggcgtattc aacgtttctt gacacaacca ttccatgttg ctgaagtgtt
     7681 ccttaataag cctggtgcat acgtatcacg cgacgaaaca atcgcaggct ttaaagaaat
     7741 actatctggc aagcatgatg acaaaccaga gtctgcgttt tatcttaaag ggaatattaa
     7801 ggaagtaaaa tagatgcacg ttaccttagt aacactaagt ggcacgaggt atgacgatgt
     7861 agctaaagaa gttcgcttgg ctacaccaaa tggtgcaatg gtcgtactac cctatcacga
     7921 gcctatgaca gctgtaacaa ctgcgggtgc tgttatcatt gtagacaaag taggcgaaga
     7981 agaagtattt gcatcatacg gcggattaat ggaagtgtct gaaaattctg tccggttatt
     8041 actagacgaa gctgaccacg caagagatct tgttgaagaa gaaatcaaag aagcgcttga
     8101 atctgctatt cagctaaaag caaaagcaaa agatcaaaca gaactcaccg aagctcaacg
     8161 attaatagat cgtcacgaag tacgccttgg cgtcgcacaa cttcgccgca aaacacgtcg
     8221 ctaaacattc atagaaggtt atctagaaat ataacgataa tataagaact ctatttgctt
     8281 tttataccat aacctttata tactattggt aataagtaaa aggggtatat caaggtggat
     8341 gacaaagaaa ccaagaatat agatggcatt aaaccgccag aacaagcgca agatggctcg
     8401 tcagagacta ttaatgtaaa cgtaacagac acgagtgacc aagaagaatc agcggatgtt
     8461 gctataaata gcgatggcga catagccgat tcacccgaca gtgtcgtaac agaaaacgtt
     8521 agtgatagcg ttgaacctga agcttcttct agtgaaacca cagaaacaga agcctcaacg
     8581 atacaaagcg aggattctga taccactaca ttttcagcaa atcaacctgc tgctaatgaa
     8641 ccaacacaag ctacagaaaa ccaacctagc gtggcggatc ctggaatcgt accgtctgta
     8701 cagccgccag caaataatga acaaccgtct acagatacaa cgcgactaaa aaagaagaat
     8761 aagcaactca aaatatgggt tgtcgtgctt atgctattgt tggtagcagt tggtagtgca
     8821 ctggttgtat attttgctca acaaaataaa tctaaaagcg acctcgaagc gcaacaacaa
     8881 accaatgcta gcctgcaaca acagcttaat cagcaacagc agaactctac gcagcagact
     8941 atagacgatt tgactacaaa gcttaaagaa gaacaaacaa aaaataccga actacaagca
     9001 acaatagact cacaaaacaa gcaaatacaa gaataccaaa cggctattaa aaagatgctt
     9061 gctgcttgtg gttcagcgtg tagtagtata gattacccta cgtctacaga ggcagatact
     9121 aacgcaacaa attagtgtaa ttttaaagtg tatagtagcc ttagactgct ggtatactag
     9181 tactagcaaa aacaataaat ctatgtgtaa ttgagaggaa aaaatggacg atattaagcc
     9241 cccacgcaat tttggctcag gccccacacc tacaaattat ggacgtaccc ctacgcaaca
     9301 aacaggaaac gtcccaccgt ctgggcaaca gtatgctgaa tcacatgcac ctgaccagca
     9361 aagccatgat caggtacctg ctgaatcgta caaactacca caaaagaaga caggtaaaaa
     9421 acgtactatt gcactatggg ttcttgttgt gctcacgctt gcagcaggtg catttgctgt
     9481 atgggagttt atgcaaattc gtgatcttca gacgcaagta gatgaactga agcaagagaa
     9541 tgcaagactg acagaaaaag tgtattccat aaattacgac aaccgtgact tacggcaaaa
     9601 actagagctt acaacaactg aaaacgattc actaaaagaa cttaacgata aattacttga
     9661 aacgtgtgga gcggcctgta gcacaataat cccctagcca ctctttttac gcctagcgat
     9721 ttacctccca ttctgttata aatagaacaa agtatgcagc aaaccgaaat tactattccg
     9781 gatacgtatg tatctaggct caacataaat ggtctgcgcg ggcgagtatt agatataccc
     9841 agcaagcgtc catctaaaca cgggaaaaat atcctgcttg tctatgggca tcattcaagc
     9901 atagaacgca tgtacagcct tgcatcttat ttaagtacct atggtacggt tacgatgcct
     9961 gatttgccag gctttggtgg tatggatagt ttttatatta ttaataaaaa acccactctt
    10021 gatgccatgg cggaatatct tgcaacgttc atcaaacttc attacagaaa taaacctata
    10081 gctatttgtg gcatgagtta tgggttttta gtcgtaacaa gaatgctaca aaagtatcca
    10141 cacatgcaca agcaggtcaa tctactggtg agtcttgttg gattttctaa aaaaaatgat
    10201 tttagattta aaccagcaac atataacacg cttctgtacg gatcaagatt tatgagtacc
    10261 ctgccgtttt cttttttggc aaaacatcta ttttttacta aaccacttat taaagcatcg
    10321 tatgctatta ccgccaaaag tcatgtaaaa atgatggatg ctacgcctga agagcggcaa
    10381 aaacgtatag attttgaagt atatctgtgg aaatgtaacg atgcaagaac atattttgct
    10441 acgtcactgt gttttttgac agttaatctt accacccaga aaataccctt agcattgcac
    10501 catgtttctg taaagcaaga tcagtacttt gataaagcaa tcgttttaaa aaacttacga
    10561 cgaatttatt ctaaagttag tgcctatacc gcaagcttgc ctaaccacgc ccccacagtc
    10621 attagtgacg aagctgatgc agcattacta atcccgcagc gtcttaaggt acttcttaaa
    10681 caatatcaga agagtaaggt ataatgaagt atatgaaaat tggcatcgtc tgcccctaca
    10741 atatgttcca atttgcgggc ggagtacaag acatagtggt gaatctgcac acttacctgt
    10801 cagcgcaagg acatagtgta aaaatcatca cccctcgccc tcgggcacac caagactctg
    10861 tgagtgatca ctatatattg gttggtcgta gcgccaagat gaatacaccc tttaatacaa
    10921 tggcagatat tggctttgaa gcagacggag atgaaataca ggctatttta gacagagaac
    10981 agtttgatat tatacatttt catgaaccgt gggtgccagt attatcaagg caaatattat
    11041 cacgttcaaa atcaataaat gtcgcgacat tccacgctaa gctaccagaa agtttattat
    11101 cgaaatctat tattagcact gtcgtgcctt atacaaaatc aatcttaaag tatatagata
    11161 catacactgc tgtgtcgcca gctgcagccg aacatttgca aagccttagt gatgaacatg
    11221 tagaaataat accaaatggt atacgactag agcagtttac acataccaac cacctaacac
    11281 gcaagaacaa aaaaaagcgt atcgtctatt taggacgttt agaaaaaaga aaaggtattg
    11341 aacatcttct aagtgcgtat gcttcactgc gcgaatcgca caaagacgta gagctcatta
    11401 ttggaggtaa tggtgttaag aataatatgc tacaacgcat tgtcgcccag tatgaaatac
    11461 cagacgttac gtttcttggg tatgttacag aagaagataa accataccta cttgccagtg
    11521 ctgacgtatt ttgctcaccc gctttgtatg gtgagagttt tggtatagtt cttttagaag
    11581 caatggcggt tggtacgcct gtagtagccg gtaacaatag tggttacgcc agtgtcatga
    11641 ctggcaaagg gcggct
//
LOCUS       DAOPLS010000003        11525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_5883_length_11525_cov_5.040017, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000003 DAOPLS010000000
VERSION     DAOPLS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5883_length_11525_cov_5.040017"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..118
                     /locus_tag="PKV52_00135"
     tRNA            42..118
                     /locus_tag="PKV52_00135"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Lys,seq:ttt)
     gene            223..504
                     /locus_tag="PKV52_00140"
     CDS             223..504
                     /locus_tag="PKV52_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPD98654.1"
                     /translation="MIQDIKKRATHRSKIIEGQFKGLVKAIEKEEYCIDILTQSLAIQ
                     QSLRSLNKLVLENHMKTHVKEGMTEGDAKTQQEIIDEMLKIYDLTNVRG"
     gene            509..2521
                     /locus_tag="PKV52_00145"
     CDS             509..2521
                     /locus_tag="PKV52_00145"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HPD98655.1"
                     /translation="MDHHEHNHHMNHSEHGGHDKHAGHNPDMFKQKFWLSLLFTLPVL
                     YFSQTIQDLLGYNAISFTGSEYIPAIFGVIIFFYGGLVFLRSAKAEIANRQPGMMTLI
                     SLAISVAFVYSSLITLNIVDGMDFWWELASLVTIMLLGHWLEMASVMNAQGALNELAK
                     LLPDEAELVTKSGTKVVSVAELKIGDKVLIRPGSQIPVDGVVTKGESKVNESMLTGES
                     KPVDKSKDSELAAGTVNKSGSLTMKVTKTGDDTALAGIMKLVSEAQSSKSKTQILADR
                     AAAYLFYIALVAAFVTAIGWSFAGESGGYTLERVVAVLIIACPHALGLAIPLVTAIST
                     SKAASAGVIVRERSALELVRNVDVVLFDKTGTLTTGEQGVVEIVADDKKELLAIAAGI
                     EQDSEHPIAKAIVTKAKDMNVKIKSANTFSALEGRGAKAKIGTATFYVGGPRMLKEQK
                     IKLNPKLQKATDKVHENGQTVVYTITGKEVLGAFMIADTIREESKSAVKTLRKAGKKV
                     AIVTGDSKGVASWVADQLGIEDYHAEVLPENKSDVVKNLQKDGMKVAFVGDGVNDAPA
                     LTQADVGIAIGAGTDVAIESAGIVLASSDPRGVSRIINLSKATYRKMQQNLVWATGYN
                     VMAIPLAAGALAGIGFVLSPAIGAVLMSLSTIIVAFNAQLLRTKEV"
     gene            2523..3383
                     /locus_tag="PKV52_00150"
     CDS             2523..3383
                     /locus_tag="PKV52_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98656.1"
                     /translation="MAKQEEIKDEKVVEEVKDTKSSSNKKTWFIKGGLVALAVLLLSG
                     GYYGYLYASTPEHLRNPEFEHYHLRTQIVVNGDPVDFSHHDFQEDYDKTSCSAELTGQ
                     PIDFHDEMDQMAHVHWSGITGGEFLKFYGWNLIGGEDDSLGRRYDQGMMRMHHVRTAG
                     DLLPDIPEGTNFYVYIGDENSYEQKDWIDFLNMDLEDFLGKKSYLNTDEETSFNPLNW
                     LTQKAYAHGMEDDGHDETELENDEARLERINNLIGNVVIFVQDTEPTEQQVQERFANL
                     VPLSDSVCGG"
     gene            3386..4264
                     /locus_tag="PKV52_00155"
     CDS             3386..4264
                     /locus_tag="PKV52_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98657.1"
                     /translation="MNNKIFWGVVVVVVVIFGFFVLSGSDSTETNEASDAVSLTLSDI
                     HGLEVDINDPDRLYLPNHQGLYVQESDGEIKKQSEISDDLMSFAVHPSDENIFYASGH
                     PRTGGNFGLIKSVDGGVSWQDVSEGLNGPVDFHTMAVDSANGDHIYGYYAGALQRTLD
                     GGETWQYLNDAPDKIIQLSAGNKQDQMYAATTSGLYRSNDQGNTWELAAFESETVIAI
                     EVNPSSDQMLANTNAQGLVLSNDGGETWEKSDFSTSDTVLYIASSASEPTKSYLITKS
                     LKIFQSTDNGSTWSAR"
     gene            complement(4331..4924)
                     /locus_tag="PKV52_00160"
     CDS             complement(4331..4924)
                     /locus_tag="PKV52_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98658.1"
                     /translation="MNLKIVIAVIAIIIVVGLGYMIFNTYEDNKTVSQQSITQLEENT
                     TAQNCVSDDCLKVENLEYPVDKLPDSVAQALYSALDDEYKAHATYAAVINTLGPIRPF
                     SMIIRAEEQHISSLKALFDKYALSIPADPYANIMAASSKADNCAVGVQAEIDNASLYR
                     DTLLPAVSNYPDITSVFTSLMNASQNNHLPAFERCAS"
     gene            5034..6008
                     /locus_tag="PKV52_00165"
     CDS             5034..6008
                     /locus_tag="PKV52_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPD98659.1"
                     /translation="MKAAVNETYGPPSVIKIKGVKKPTPRPNEILVEVHYSSVNRTDV
                     GFLRAKPFVTRFFTGIIKPKYLTLGCEYSGIVKDLGKDVKKFKLGDRVFGFDDSNFGG
                     YAEYKIVNENQMVETIPSNTTLEQAAVALEGAHYALFYIYKVPESKKTRVFVNGATGA
                     IGSAGVQILKALGYYVEASSTTKDIGKVKKLGADKVIDWEKQDITRVASKCDVYFDAV
                     GKSSFKQAKKILNPHGIYMSSELGPYGQNPLLALFNVVQRLFTKRNVYFPIPKTRLKE
                     ATIIKQYLANGTFIPLVDRTYSLVDIQKAFQYVESGKKVGNVVIKVKK"
     gene            6096..7115
                     /locus_tag="PKV52_00170"
     CDS             6096..7115
                     /locus_tag="PKV52_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HPD98660.1"
                     /translation="MNKNIATKNQPNNIVFSKTDGLHAALNMIPAFILILSGNLTAGI
                     AFALGTIPASQLGVAPNRKKRIVFSVISCVFGLGIIGGSLVLHNMSLWLAAIAFFIVA
                     FVASVIAAKKPIGIVMLAFFLPALAVGYSYSPSVGLSLGLVFILGSLWSGLTSMLWPE
                     SKIQNSADGAKAFVSSNPKIYGVLLGLTASVALVLGTYIDPVFIGWTATSALLIMRPL
                     RGMVGLRGLARTLSTIIGTLLAIITVQLDLPALVTALLISLMMIFIIGTRLSRWYIVP
                     MGTAFLILTLQLYEEQNINAIRQLGYSRILDNILGAAIALFFGLLVPLMLIKIQKHSH
                     TKAGT"
     gene            7202..7753
                     /locus_tag="PKV52_00175"
     CDS             7202..7753
                     /locus_tag="PKV52_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPD98661.1"
                     /translation="MPAITFITGNQSKADYVSRYLGFKVSHRKIELEEIQSLDLRVVV
                     EHKVKQAYAIINAPVLVEDVSLEFKALGRLPGTFIRFYVDEVPFETICRTLDGLSREA
                     TARCVFGYYDGNKLRLFESSLDGTIAMHPKGENGFGWDKIFIPQGYNVTRASLDEEAN
                     KITYLKIKPFEQLKSFLIATQPD"
     gene            7887..8819
                     /locus_tag="PKV52_00180"
     CDS             7887..8819
                     /locus_tag="PKV52_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPD98662.1"
                     /translation="MIKYYLRKSKHEEVVTSLSYEPGQWVQLIAPTEEEIHYISRELS
                     LEPSLLLDATDMDEMPRFEIDDDTAYLYTRFAWKNAGGTIHTEPVLFVVGKGVFVSLT
                     NRSFPALEDLLIRQKHLVTNRPKHLLLPVLNVSLQSYASQLAHIGRQIRTARNALRDE
                     KFSNKHFVRFVEIEDFLNDFLSELVPTNNILLNMVAGRKAFSFTETDHDILEDLQLET
                     AQLIDESKGYLKTIVNIREAYSNVMTNNLNRQIKILTTLTIVLTVPTIVGSFFGMNVG
                     IPLDNNPLAFPLIVGTTFGVAFLVLYFLKRKDWL"
     gene            complement(8864..10111)
                     /locus_tag="PKV52_00185"
     CDS             complement(8864..10111)
                     /locus_tag="PKV52_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98663.1"
                     /translation="MYSYRKPIRRKKSRKSKSLYVTAMVLAVLGIFAAKLLVIFPTVY
                     ATADIQDVKLANTLAIPWPAYGQSAVGVKGHGVVSASPNQSARPTASVAKLVTALAIL
                     EKKPLVNNEQGEQIVISDADIAIYDFYLSHQGVVVDIKPGQSISQYKALQYMLILSAN
                     NIADISAKWAFGSVDEYTAYANDMVKKMGLSTMHIDDASGLSPKTISSAEDLVRLGEY
                     ALDNPVIADIVAQESIDLPEGTQKINTNVFLNYQGNGVIGIKNGLTDEAGGVLLAAAK
                     RTVDNTQLTIISVVMGSERYFDSQKDAVALIDPTVSALSGSASIAAGTVVGHYDVPWG
                     ENADVITKEPITLNKWTNALDASYVELYPITTTTQKGTVVGKLTVPQDNANASEAQIM
                     LDSDVNNPSTKWRIMGLINKTSY"
     gene            10338..>11525
                     /locus_tag="PKV52_00190"
     CDS             10338..>11525
                     /locus_tag="PKV52_00190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPD98664.1"
                     /translation="MSFNRNRRGPSGSFGGPGRSFKNNNRRGAGSKKRTGEYINPAKF
                     VQAAKPSTQTEYVAQNSFDDFAINDVIKTNLASGGYSTPSPIQDQTIAYGIDGHDIVG
                     VANTGTGKTAAFAIPIIHNAMRFSDAYALIVAPTRELAQQIEQEAKKIGKNSGLHSAL
                     LIGGMPMGPQLGQLRAKPQIVIGTPGRIKDHIERQSFDVSKFNMVVLDEVDRMLDMGF
                     LPDVKHILQQLSPERQSYFFSATLDQRVKGLITQFAPKHISVSVKTAPSSENVDQNVV
                     QYGTTSHKIEKLHDILISHQDIKVIVFDETQRNVERLSKELQARGFSATAIHGGKTQG
                     QRKRALDEFKANKAKILVATDVAARGIDVKDITHVINYSLPQTYDDYVHRIGRTGRAG
                     KTGY"
BASE COUNT         3534 a         2215 c         2447 g         3329 t
ORIGIN      
        1 attgattttc tacatctgtt atgctttact attctagttg cgggcgatta gctcagctgg
       61 ttagagcacc tgccttttaa gcagggtgtc ctgggttcga gtcccagatg gccctccaaa
      121 gtaaattaca acatttgtaa ctcaaattcg cctcaaggca ggggcgtttt ttagttgcat
      181 gaaaagttgc attgataccc cctgggggta tacaatgagt ttatgattca agatattaaa
      241 aaacgcgcaa cacacaggtc aaagattatt gagggacagt ttaagggact ggttaaagca
      301 attgaaaagg aagaatattg catagatatt cttactcaaa gtttagctat ccagcagtcc
      361 ctgcgctctc ttaataaact cgtcctagaa aaccacatga agacccatgt taaagaaggt
      421 atgactgagg gagatgcaaa aactcaacag gaaattatcg atgagatgct gaagatttat
      481 gatttaacaa atgtaagggg ttaatttcat ggatcatcac gagcataacc atcatatgaa
      541 tcattcagag catggaggcc atgataaaca tgcgggccat aaccccgata tgtttaagca
      601 gaaattctgg ttatcgctac tgttcactct gccagtccta tatttttccc aaaccattca
      661 agatttattg ggctacaacg ctatttcgtt cacaggaagt gaatacattc cagcaatttt
      721 tggtgtaatt attttctttt atggaggact tgtttttcta aggagcgcaa aagctgaaat
      781 tgccaaccgt cagccaggca tgatgactct tatctcactg gcaatcagtg tcgcttttgt
      841 gtatagctca cttataacac tcaacattgt agatggcatg gatttttggt gggagcttgc
      901 atcactggtc accatcatgt tgcttggtca ctggcttgag atggcttctg ttatgaacgc
      961 acaaggcgca ttaaacgaac ttgctaagct tctacctgac gaagccgaat tagtgacaaa
     1021 atcagggact aaggtagtgt cggttgcaga gctgaaaatc ggtgataaag tcctcatacg
     1081 acccggctcc cagataccag ttgatggcgt agtgactaaa ggagagtcaa aagttaacga
     1141 gtccatgctg acaggtgaat cgaagccagt agacaaatca aaagacagtg agttggctgc
     1201 cggaacggtt aataaaagtg gctcacttac tatgaaagtc accaaaactg gagatgatac
     1261 agctttagca ggaataatga agcttgtttc tgaagctcaa agtagtaaat ctaagacaca
     1321 gattcttgct gacagagccg cagcctatct tttctatatt gcactcgttg ctgcatttgt
     1381 aactgcaatt ggatggtcat tcgctggcga atcaggcggt tacacacttg agcgagtagt
     1441 ggcagtacta attattgcct gtccgcatgc cttaggtctg gcgattccac tcgtaacagc
     1501 aatttctacg agcaaggcag cgagtgcggg agtaattgtg cgagaacgaa gcgctttgga
     1561 gctcgtgcgt aatgtagacg tcgtattgtt tgataaaacc ggaactctaa caactggtga
     1621 gcaaggtgtg gttgaaatag ttgctgacga taaaaaagaa ttgctcgcta ttgctgcagg
     1681 tatagaacaa gattcggaac acccaatagc caaagcaata gtcacaaaag ccaaggatat
     1741 gaacgttaag ataaagtcag ccaatacttt cagtgccctt gaaggtcgtg gcgccaaggc
     1801 aaagattgga acggcaacat tctatgttgg tggtcctaga atgctaaagg aacaaaaaat
     1861 taaactgaat cctaaactgc aaaaagctac cgacaaagta cacgaaaatg gtcagacagt
     1921 tgtctacaca ataactggaa aagaagtttt aggtgctttt atgatcgcgg ataccattag
     1981 agaagaatct aaatccgcag ttaagactct gcggaaagct ggcaaaaaag ttgcaatagt
     2041 cactggcgac tccaagggtg tggcttcttg ggtggctgat cagctcggta ttgaagacta
     2101 ccatgccgag gtgttgccag aaaataaatc agatgtcgta aagaatttgc aaaaggacgg
     2161 catgaaagtt gcctttgttg gagatggtgt aaatgatgcc ccagcactta cgcaagccga
     2221 tgtcggtatt gcaattggtg cgggtacaga cgtggctatt gaatcggctg gtatagtgct
     2281 tgcaagcagc gacccacgag gggtgtcgcg gatcataaat ctatcaaagg caacgtatag
     2341 aaaaatgcaa cagaatcttg tgtgggcgac tggttacaac gtcatggcta tcccactagc
     2401 tgctggggcg cttgctggaa taggttttgt tctgtcacca gcaattggcg ccgtattaat
     2461 gtcgctttca acaattatcg ttgcatttaa tgcacagcta ctaagaacta aggaggtgta
     2521 agatggcaaa acaggaagaa ataaaggatg aaaaagtagt ggaagaagtt aaagatacta
     2581 aaagctcttc caataagaaa acatggttta ttaaaggcgg actggtagca ctggctgtac
     2641 tgctgctgag tggtggttat tacggttact tgtatgcatc aacccctgaa cacctgcgaa
     2701 accctgagtt tgagcattat catttgcgga ctcaaattgt tgtaaatggc gatccagtcg
     2761 acttctctca ccatgacttc caagaagatt atgataaaac ctcttgtagt gctgagctga
     2821 ctggtcagcc aatcgatttc catgacgaaa tggaccaaat ggctcacgta cactggagcg
     2881 gtattactgg tggtgaattc ttgaagttct acggctggaa tcttattggt ggagaggatg
     2941 attcacttgg tcgacgttat gaccaaggaa tgatgcgcat gcatcatgtt agaacagctg
     3001 gcgatctgtt gccagatatc ccggaaggaa ccaatttcta cgtctatatt ggtgatgaaa
     3061 acagttacga gcaaaaagac tggatcgatt ttctcaatat ggacctcgaa gatttcttgg
     3121 gcaagaaaag ctacctaaat actgacgagg aaacctcgtt taatcctctc aattggctta
     3181 cccaaaaagc ctatgcccac ggcatggaag atgacggtca tgatgaaaca gagcttgaaa
     3241 atgatgaggc aaggttggag cgtatcaaca atctaatcgg taatgtcgtt atattcgtgc
     3301 aagacacgga accgaccgag cagcaggtac aagaacgatt cgctaaccta gtaccattaa
     3361 gtgatagtgt ttgtggaggt tagagatgaa taacaagatt ttttggggag tagttgtagt
     3421 tgttgtcgtc atattcggat tcttcgtcct ttcgggtagc gatagcaccg agacaaatga
     3481 agcatccgac gctgtgtcgt taacgctatc agacattcat gggcttgagg tagatattaa
     3541 cgatccagat cgactctatc tgcccaacca tcagggcttg tatgtgcaag aatctgacgg
     3601 agaaattaag aagcaaagtg aaatatccga tgatttgatg agttttgccg ttcatccatc
     3661 agatgaaaac attttctatg ccagtggaca cccacgaact ggtggtaatt ttgggcttat
     3721 taagtcggtt gacggtggtg tgtcgtggca ggatgtatcg gaaggcttaa acggaccagt
     3781 tgattttcac actatggctg ttgattcagc taatggtgac catatatacg gttactatgc
     3841 gggagcactt caacgaactc ttgatggtgg cgagacttgg caatatctca atgatgcgcc
     3901 cgataagatc attcagctca gcgcaggaaa caaacaagac caaatgtatg ccgcaacaac
     3961 aagtgggctc tatagaagca acgatcaggg taacacatgg gagctagcag cattcgaaag
     4021 cgaaacagta attgccattg aggttaaccc ttcatcggat cagatgcttg caaatacgaa
     4081 cgcgcaaggt ttagtgcttt ccaacgacgg tggcgaaact tgggagaaga gcgacttcag
     4141 cacatccgac actgtgctct acattgcgtc ttctgcttca gagccaacaa agtcatacct
     4201 cataacaaaa tcactcaaga ttttccaaag caccgataat ggtagtactt ggtctgcacg
     4261 ctaatcgcta gacagtctgc gcctcaattt tagttgcaac aagaagtacg gttacctaag
     4321 gcttttgcaa ttacgatgcg catcgctcaa atgcgggtaa atgattgttt tgtgatgcat
     4381 tcataaggct cgtgaatact gaagtaatat ctggatagtt tgaaactgca ggtagcaatg
     4441 tgtcgcgata taatgatgcg ttatcaattt ctgcctgtac accaactgcg caattgtcag
     4501 ccttagatga agcagccatt atattggcgt atgggtctgc cggaatgcta agggcgtatt
     4561 tgtcaaagag cgccttaagg gatgatatat gttgctcttc tgctcttatt atcattgaaa
     4621 acggacgaat gggacctaat gtattaatta ctgctgcgta tgtagcgtga gctttatact
     4681 catcatctaa tgccgaataa agagcctggg cgacactatc tggtagttta tctacaggat
     4741 attctaggtt ttcaactttc aggcagtcat cagaaacgca attttgagct gtggtgtttt
     4801 cttctagttg tgtaatactc tgttgcgata ctgtcttatt atcttcatag gtattaaata
     4861 tcatatatcc aagcccgacc actataatga tggcaataac tgcgataact atttttaagt
     4921 tcatactttc ctttcagtat cacttatact taggttatta ttctatatca tactgaataa
     4981 tgcctgaatg caaccaataa ttgttacata aataattttg atacaatgaa ccaatgaaag
     5041 ctgctgtaaa tgaaacctac ggtcctcctt ctgtcattaa gataaaaggg gttaaaaagc
     5101 caacaccaag gccaaatgaa attttggtag aagtgcatta ttcgtctgtt aatagaacag
     5161 acgtaggatt tttaagggcc aagccttttg taactcgctt ttttacagga ataattaaac
     5221 caaaatatct tacgctaggg tgtgaatatt ctggaattgt taaagaccta ggtaaagatg
     5281 taaaaaaatt caagttaggc gatagggtgt ttgggtttga cgatagtaac tttggaggtt
     5341 acgcggagta taagattgtt aatgaaaacc aaatggtcga aaccattccg agtaacacaa
     5401 cacttgaaca agcagctgta gcgcttgaag gcgcccacta tgcccttttt tatatatata
     5461 aagttcccga atctaaaaaa actagggtgt ttgttaatgg ggcaaccggt gctattggtt
     5521 ctgccggtgt acaaattcta aaagcattag gttactacgt agaagctagt agcactacta
     5581 aagatattgg aaaagttaaa aaattgggag cagacaaggt aattgactgg gaaaagcaag
     5641 atatcacgcg cgtagcatct aagtgcgacg tatactttga tgcagtagga aaaagttctt
     5701 tcaagcaagc taaaaaaata ctcaatcccc acgggatata tatgtctagc gagcttggac
     5761 catatgggca aaatccacta ttggcgctgt tcaatgtagt tcaaagacta tttacaaaac
     5821 gtaatgtata ttttcctatt ccaaagacca ggttaaaaga agctacgata attaaacaat
     5881 atcttgcaaa tggtacattc atacctctag tagacagaac atatagcctc gtagatattc
     5941 agaaagcttt tcaatatgta gagtctggca aaaaagttgg caatgtcgtt ataaaagtaa
     6001 aaaaatagta ttaccataac agttgagtca gcacttttct taaaaaatat tttttttgga
     6061 aattaaataa cactgtattt gctatagtta taacagtgaa taaaaacatt gccacaaaga
     6121 atcagcctaa taatatagtt ttttctaaaa cagatggctt gcacgccgcg ctcaatatga
     6181 tcccggcatt tatattaatt ttatctggca atctcacggc aggcattgca tttgctcttg
     6241 gtacaatacc ggcatcacag cttggcgtgg ccccaaatcg taaaaaaaga attgtgttta
     6301 gtgttattag ttgtgttttt ggattaggta ttattggtgg atctcttgta ctgcataata
     6361 tgagcctatg gctagcagcg attgcctttt ttattgtagc ctttgtagca tctgttattg
     6421 cagccaaaaa gccaattggt attgtaatgc tcgcgttctt tttaccggct ttggctgtag
     6481 gttatagcta cagtccgtct gttggtctta gtttgggcct agtttttatt cttggttcgt
     6541 tgtggtctgg gctaacttct atgctatggc cagaatctaa aatacaaaat tctgcagatg
     6601 gagcaaaagc atttgtatct agcaacccta aaatatacgg agttctgctt ggcttaactg
     6661 cctcagtagc tttggtgcta ggaacctata ttgaccctgt tttcattggc tggacagcaa
     6721 catctgcgct tttaattatg cgaccgttac gtggcatggt gggcttacga ggactagcca
     6781 gaacactatc tacaataata gggaccttgc tagcaattat tacagtacaa ctagacttac
     6841 ctgcgctagt gacggcatta cttattagcc tgatgatgat atttatcatt ggcacccgtt
     6901 tgagtcggtg gtacatagta ccaatgggta cagctttcct aattttgaca ttgcagttat
     6961 atgaagagca aaacataaat gcaattagac aattaggcta cagtagaata ctagataata
     7021 ttcttggtgc tgccattgcc ttattctttg gtttgcttgt accacttatg ttaattaaga
     7081 ttcaaaaaca ctcacacact aaagctggca cataataaat taatgcacgg tggttatgag
     7141 tattgaaaat agatagtttt taagcaccta cttttatatg tggttttgct ataatcagct
     7201 aatgccagca atcacattta taaccggtaa ccaatcaaaa gcagactatg tatctaggta
     7261 tttaggattt aaagtcagcc accgtaaaat tgaactagaa gaaatacaat ctttagacct
     7321 acgagtagtg gtagaacaca aggtcaaaca agcatatgca attatcaatg cgcccgtact
     7381 cgttgaagac gtgtcgttag agttcaaagc gcttggtagg ttaccaggta cttttatacg
     7441 attttatgta gatgaagttc cgtttgaaac aatctgcagg acattagatg gtttaagccg
     7501 tgaagcgaca gcgcgttgtg tatttggtta ctacgacggt aacaaattac ggttgtttga
     7561 gagtagtctc gacggcacca tcgcaatgca tccaaaaggc gaaaatggtt ttggctggga
     7621 caaaatattt attccacaag gatacaacgt aacaagagca tctctagatg aagaagctaa
     7681 caaaatcacc taccttaaaa taaaaccatt tgaacaatta aagtcttttc taatagctac
     7741 gcagccagac taagcatgta gctatcatat gcatggtact tattacttta cttaagaaaa
     7801 gcaatctaag tgatatcacc tactacaagt aattctattg caaatattag cacttatata
     7861 aacagtaatc tggtactatt aagtgtatga ttaagtacta cttaagaaaa agcaaacatg
     7921 aagaagttgt aacttcgttg agttacgaac ccggccagtg ggtgcagtta attgctccaa
     7981 cagaagaaga aatacattat atatcgcgtg agctttccct agagccatca ctgctgcttg
     8041 atgcgacaga tatggatgaa atgcctcgtt ttgaaataga tgatgatacc gcctatttgt
     8101 atacgcgctt tgcatggaag aatgccggtg gcacaattca tacagaacct gtgctttttg
     8161 ttgtcggtaa gggtgtcttt gtgtctttga caaatcgtag tttccctgct ttagaagacc
     8221 tgcttatacg gcaaaaacac ttggtcacga atcggcctaa acacttgtta ctacctgtat
     8281 tgaatgtgtc tttacaaagt tacgcttcac aacttgccca tataggcagg caaatacgca
     8341 ctgcccgcaa cgccctgcgt gacgaaaagt tttctaataa gcattttgta cgttttgtag
     8401 aaattgaaga ttttttaaac gattttctga gtgaacttgt acctacaaac aatattttac
     8461 ttaacatggt tgctggcaga aaagcgttta gttttacaga aacagatcat gatattttag
     8521 aagatttaca actagaaacc gctcaattaa ttgacgaaag taaaggctat ttaaaaacta
     8581 ttgttaacat tcgtgaagcg tacagcaatg ttatgaccaa caatttaaac cggcaaatta
     8641 aaatccttac aacgcttacc attgtactca ctgtcccaac cattgtgggc tctttttttg
     8701 gtatgaacgt tggtattccg ctagacaaca acccccttgc ttttccactg attgttggaa
     8761 ctacctttgg tgtagccttt ttagttttat attttcttaa acgcaaagac tggctttaat
     8821 actagccatt taagctcaat ataacgagtt gttatattta gttctagtat gatgttttgt
     8881 ttattaaacc catgatgcgc cactttgtac ttgggttatt aacatcagaa tcaagcatta
     8941 tctgggcttc gctagcattg gcattatctt gggggacggt cagcttgcct acaacggtac
     9001 ccttctgagt tgttgttgtt atggggtata actcaacata tgaggcgtct agcgcatttg
     9061 tccatttatt taaggtgatt ggttctttgg ttattacgtc tgcattttct ccccatggta
     9121 catcgtagtg cccaacaact gttcctgccg caatactggc tgaaccagag agtgcactca
     9181 ctgttggatc aataagtgcc acagcatctt tttgtgaatc aaaataacgt tcagaaccca
     9241 tcaccacact aatgatggtt agctgagtat tatcaacagt acgtttggca gctgctaata
     9301 atacaccgcc tgcttcatct gttaagccat ttttaatacc tataacaccg ttaccttggt
     9361 agtttaaaaa aacattggta ttaatttttt gcgtaccttc tggtaaatca attgattctt
     9421 gggcgacaat atccgctata actgggttat ctaaggcata ttcgccaaga cgcactaaat
     9481 cttctgctga cgatattgtt tttggtgaca atccacttgc atcatctatg tgcatagtgc
     9541 ttaaccccat tttttttacc atatcgttcg catacgctgt gtattcatct actgatccga
     9601 acgcccattt tgcagatata tctgcaatgt tattagcgga tagtataagc atgtattgca
     9661 acgctttgta ctgtgaaata gactgtcctg gtttaatatc tacaactaca ccttggtgac
     9721 taaggtaaaa atcataaatg gctatatcgg catcagaaat tacaatttgt tcaccctgct
     9781 cattatttac aaggggcttc ttttctaaaa ttgccagtgc ggttaccaat tttgcaacac
     9841 tcgccgtagg ccgtgcgctt tggtttgggc tagcacttac aacaccatgc cctttaacac
     9901 caacagcaga ttggccatat gcaggccaag gaatggcaag tgtattggct aatttaacat
     9961 cttgaatatc cgcggtcgca tatacggtag gaaatataac aagtaatttc gcggcaaata
    10021 tacctaacac cgccaatacc atagcggtaa catataaact tttcgattta cggctttttt
    10081 tacgacgaat aggtttgcga tacgaataca tacatcatct attttagcag agtaaaaaat
    10141 actcgttatt aagacctcat agcgtcatat atgtgattgc gcaagtatct cagtacatat
    10201 tatggttgct tttttaagat aataatgcta aagtataatc tgaattgttt aagatgaatc
    10261 caagattagt actgttaata cttaggtttc attaccacaa atcaaagtat taaactaaac
    10321 aaaaggattc attttttatg tcttttaacc gtaatcgccg tggaccaagc ggctcttttg
    10381 gcggccctgg ccgttcattc aaaaataata accgtagagg tgccggttct aaaaaacgca
    10441 cgggtgaata tattaaccca gctaaatttg tgcaagcagc taaaccatct acccaaacag
    10501 aatatgtcgc gcaaaatagc tttgatgatt ttgccataaa tgacgttata aaaacgaatt
    10561 tggcaagtgg tggctatagc acgccatcgc ctattcaaga ccaaacaata gcctacggca
    10621 tagatggtca tgatattgtt ggcgtagcta acacgggcac tggtaagacg gctgcctttg
    10681 ctatacctat tattcataac gctatgcgct tttctgatgc atatgcattg attgtagcgc
    10741 ccacacgtga gcttgcgcag caaatagaac aagaagctaa aaaaattggc aaaaacagtg
    10801 gactgcatag tgccctgtta attggtggca tgcctatggg cccacagctt ggccaattac
    10861 gagcaaaacc gcaaattgtt attggaacac ccggcagaat caaagatcat atagaaagac
    10921 aatcgtttga tgttagtaaa tttaatatgg ttgtactaga cgaagtagac cgtatgttag
    10981 atatggggtt cttgcctgat gtaaaacaca tattgcaaca attgtcacca gaacgtcaga
    11041 gttacttctt ttctgcaacg cttgaccaaa gagtcaaagg gcttattacc cagtttgcgc
    11101 caaaacatat ttctgtttct gtaaaaacag caccaagtag cgaaaacgta gatcagaatg
    11161 tcgtgcaata tggcacaaca agtcataaga tagaaaaatt acatgacata ttaattagtc
    11221 atcaagatat aaaagtaata gtatttgatg aaactcaaag aaacgtagag cgactatcta
    11281 aagaactgca agcgagaggc tttagcgcaa ccgctataca tggtggcaaa acacaaggcc
    11341 aaagaaagcg tgcattagac gaatttaaag caaataaagc caaaatactc gtagctactg
    11401 acgttgctgc tcgtggcatt gatgtaaaag acattacaca tgttattaac tactctctac
    11461 ctcagactta cgatgattac gtacaccgta taggacgtac aggccgagcc ggcaaaacag
    11521 gctat
//
LOCUS       DAOPLS010000004        10057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_7028_length_10057_cov_3.616177, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000004 DAOPLS010000000
VERSION     DAOPLS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10057
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_10057_cov_3.616177"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1005)
                     /locus_tag="PKV52_00195"
     CDS             complement(40..1005)
                     /locus_tag="PKV52_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98665.1"
                     /translation="MAEILQGNIPKNEADANPESARRAKLKRIFGVIAIFGFLGNAGV
                     AYLGYQQLNNTEKSNAESTSIGRLKQNLPRLTECDGTTLHTREELDSEDIASMCETLI
                     KVMSTLGIDEDIYYDDIPEGHNRSAFVETSKGEFIVLEASPYNIPANKTQDVLLHEAI
                     HAAYDELVNEVLENEENNIEGRADFVLNNLVSAFRSLQKINNDISSNGIEKNSDQREV
                     CTTVWSTFTEGCFARDFSGDDTTYGHPFDDITELTASGTNLLAGLDKDASKWWFRTSL
                     KNADKAEKTTIRQAIIAVLDFIFYVTEGQPEKIVKDYDSLMTKYS"
     gene            complement(1077..1292)
                     /locus_tag="PKV52_00200"
     CDS             complement(1077..1292)
                     /locus_tag="PKV52_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98666.1"
                     /translation="MSENTQSLPLAERRAAKEKKRQHEEITRMFIVFSIGVAAIYGLI
                     TSGYIDNPLTELHQDTSITVEDTSANK"
     gene            1355..2170
                     /gene="prmC"
                     /locus_tag="PKV52_00205"
     CDS             1355..2170
                     /gene="prmC"
                     /locus_tag="PKV52_00205"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPD98667.1"
                     /translation="MTQGEWIANATKALLKCAITTARLDALVLMSYVTKQDKAAILAH
                     PETALSSADIATLATALKRRCNHEPIAYIMGTKEFYGRNFLVTPNVLIPRPESEDFIN
                     LLKHYPPTKGHKLIDIGTGSGILAISAKKEFPALSVYVTDVSKAALRVAATNAKNHAV
                     TIQCIQSNLLHTVPGCFDYIFANLPYVPKDYSVSAEVLSEPQRAIFADKNGLALIFRL
                     ADQAKKSLTKDGLIFVESLIEQQEEICDHYQKAGLTAIDNAGFIQVFKKSVMS"
     gene            complement(2208..3395)
                     /locus_tag="PKV52_00210"
     CDS             complement(2208..3395)
                     /locus_tag="PKV52_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPD98668.1"
                     /translation="MAPAPVKQSEPTPKRPKSTTSIPTRVAVIVVALAFISGIAGGFL
                     GSRLNTSRITSLDSTATQQKIASSQSELISNIAKDVSPSVVSINVESTVGNSYFYGEQ
                     TQSSAGTGFIISSDGVIVTNKHVIPENTSKVAITTSEGTEYDNVDVIARDPRSNVDLA
                     FLKVNGAKDLKPVKLGDSSQMVVGDSVIAIGYALGQFQNTVTSGIISGLGRPVTASDG
                     SATSAESLTNLFQTDAAINPGNSGGPLLNMSGEVIGINTAVAGDAENIGFAIPINDVK
                     PQITSILEKGKLEVPYLGVRYVMLTEALQQRFELARSSGAWLKAGNEAQAVVNGSPAD
                     KAGLKEGDIIFKVNGEEVTIEKPLASVLSKYNVGDEIELTYNRDGKEQTAKATIEVAP
                     STN"
     gene            complement(3427..4179)
                     /locus_tag="PKV52_00215"
     CDS             complement(3427..4179)
                     /locus_tag="PKV52_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98669.1"
                     /translation="MAKNYLQFLRPKSGVSSWLYFVLNAALPILVLVFVRLDLASIAF
                     TLVLLSKWRIFAMRPRYWLPNLRANSVDIFVGLSIVVFMSGTKILLVQLLWTIFFLVW
                     LLWLKPKSSQIFVMIQALLAQIMALIAFYQASPDHAIATGVIAVWLISYLSARHFFGA
                     FEEPYTIQLSAIWGWFAATLTWVLEHWVIIYVTVPQVALIITLVGYILALLYYLHSNE
                     KLKNSVKHQLIGFITILLIIIIAFSDWQAKTI"
     gene            complement(4194..5867)
                     /locus_tag="PKV52_00220"
     CDS             complement(4194..5867)
                     /locus_tag="PKV52_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPD98670.1"
                     /translation="MDRYKKIKPKNQYSTDGFLVTGPRKGFQPKRTPVNATPKSPRLN
                     QLDNFSGRDGFRGQRTPQMQESGDFEHIELPGDDTRATSRKSFTLDSSQSLHTPRYKR
                     LFTKNKSGKKKHKSKRRAYAKMALFFVIAGVSIFGFIFGKAWWDAHKALGGGGSALAM
                     SKDIDPNSLNGEGDGRINVLLMGKGGDKHEGGQLTDTIMIASIDPINDGVVMLSIPRD
                     LWVKPDDLWPMKINAVYNSAKEQALYTNPKDKSAAEKTGINATKKVVQDYMGVNINYY
                     AMVDFTVFEESVNTLGGITVNLPAAYSDPTMKIGKQYLNLPAGPNELDGGHALGLARS
                     RYGSFKGQESRNDFGRGENQQLVLIAIKDKALSTGTFANPLKVSQLVSAFGNRVQTNF
                     STDDLMRLYELSKKIPNENITNVDLAMKDEAVVTTGSIGGQSVVYPIAGVDDYSEVKK
                     FVRLKLKDGFIIKENPTIIVLNASGKAGAATKRADELKSYGYNVIQVADAPVSNVPVT
                     QLIDTTKGAKKYTKRYLELRFNTQAVGKADGVDISPYIADYIVIIGQNG"
     gene            5948..6781
                     /gene="lepB"
                     /locus_tag="PKV52_00225"
     CDS             5948..6781
                     /gene="lepB"
                     /locus_tag="PKV52_00225"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPD98671.1"
                     /translation="MQPNEPHNPKSFDIKPSTPPLPQPPSEHPITKEQPAQFNSAPHM
                     VNNNPVPQNIQQPDQAAQLKQRRREERQNMLYTILLFILAPLFAVLMILFVFQSYVVD
                     GSSMEPTLQNGNRVFILKLPKTMAKLQNKEYTPARNEVIVFKKPTNDGTQLIKRVIGL
                     PGDRVVIENGNVTIYNIDNPGGFNPDKGTDHENTLEPIDSDGQKVVEDVGQGELFVLG
                     DNRGPGGSLDSHSGLGLVPIDNIIGRLWVRYFPLSEFQTFSSTFANAVLYDQTLFEKY
                     S"
     gene            complement(6726..7595)
                     /locus_tag="PKV52_00230"
     CDS             complement(6726..7595)
                     /locus_tag="PKV52_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPD98672.1"
                     /translation="MSKKGLGRGFDSLVPEGTNIDGVTIPKNEKIHKLAIDIVQPKSN
                     QPRQLFDEQQLQQLALSIEAQGILQPIIVAEIEHNLYSIIAGERRWRAAQLAGLRHVP
                     AIIKDVDPLQHLELSLLENVQRADLNAIETAKTIQRLHVEYQQSYEDIALRLGKGYTT
                     VVNAVRLLQLPEPMQQALMKGDISEGHGRALLSLAKHPHAQMVLFKAIQTKKLSVRQT
                     EALAANAKLDAERTKTKDDAKKSVTSTQLSKQLTKHLGLAVRVPKSNSKGKITISYTS
                     QKEFDRIIRHLQK"
     gene            complement(7588..8346)
                     /locus_tag="PKV52_00235"
     CDS             complement(7588..8346)
                     /locus_tag="PKV52_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007201567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD98673.1"
                     /translation="MKTIAVVNQKGGVGKTTTAINVAVYIARSGKNVLLVDLDPQGNA
                     TSGLGIDKYESDKSIYDVLVNDTSPDIVRHTTIEKNLHVITSNHNLSGAEVDLVSIAR
                     REYRLSTALSAIESEYDICIIDCPPSLGLLSINGLVAADYVLIPVQTEYYAMEGIGQL
                     VQTIQRIQQTLNPTLSIVGVVMTMYDTRTSLSSQVRAEIERVFGDLVCKTVIPRNVRL
                     AEAPSHGKSIATYDKWSKGARSYKSLAKEVMQRV"
     gene            complement(8421..>10057)
                     /locus_tag="PKV52_00240"
     CDS             complement(8421..>10057)
                     /locus_tag="PKV52_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98674.1"
                     /translation="CSQSGSSGPQYAVAGSSWTIAEKTAAPAGSPGSGFLQGGNSFGA
                     AAILGTSDVNDLEIVTGGLTRITVSASGDTTIAGLATISGNLVANANTLLNGDVTIGD
                     AATDVLTINSDTLQLPNGLDIANGTLVFDAAGNKIGINASASNTLSVNTAATADSAAE
                     VLIYTNGVNQKGLVLQSVSGQLANVFEVQTDGGVPVVAASSDGLLSLGSDISGSSNQG
                     RLLLHDGINANGFTSVLGVTSLTASRNIDLPDASGTVCISGSDACGFILIAPGSAQQD
                     NSTNNSLYINKTGASGDILNLQKNGTSVLRMVNSGALQLSLTSSTAFTVKNGSGVDYF
                     NVDTNGGLIRIGGASADANGVLLVLDTKNTSGDPTGTDGGMYYNSASGKFRCYEGGEW
                     IDCIGTRQVRSFIDTTSDSAVDSNTTSYWDTSGENNNSVPNFSPSTTAKSITGSISME
                     VSSATTADRSIVARVERSIGSPAACGSGTPVGTILSTFTTNNGEQASNTMIFLDSPNT
                     TSAVYYTLCSDTATSNASSMTINRIRITLEEANNSN"
BASE COUNT         2689 a         2235 c         2035 g         3098 t
ORIGIN      
        1 ggtgttgaac taattacgtt attgcgtgct actcccaatt taagaatact tagtcattag
       61 tgaatcgtag tccttgacta tcttttctgg ctgaccttct gttacgtaaa agataaaatc
      121 aagtacagca ataatcgctt gacggattgt ggttttttct gccttgtctg cgttctttag
      181 acttgttctg aaccaccact ttgaggcatc tttatcaagc ccagcaagca ggttagttcc
      241 tgatgctgtc aactcggtga tatcgtcaaa cggatggcca taggttgtat catctccgct
      301 aaaatcacga gcaaaacaac cttcggtgaa tgtactccat acagtagtac acacctctct
      361 ttgatctgag ttcttctcga tgccattgct ggatatgtca ttgttgattt tctgtaggct
      421 tctaaatgct gatactaggt tatttaacac aaagtcagct cgaccttcga tattattttc
      481 ttcattttca agcacttcat taacgagctc atcatatgcc gcgtggatag cctcgtgcag
      541 taacacatcc tgcgttttgt ttgccggaat attatacggg cttgcttcta atacaataaa
      601 ctcaccttta cttgtttcta caaaagcaga gcgattatgg ccttcgggta tatcatcgta
      661 ataaatatct tcatctatgc ccaaagtact cattacctta ataagtgttt cgcacatact
      721 agctatatct tcactatcaa gctcttctct agtgtgaagc gtagtgccat cacactctgt
      781 caatcttggt aagttttgct tcagcctccc tattgaagtg ctttctgcat tgctcttttc
      841 tgtattattg agctgttggt aacctaaata agccacacca gcatttccta aaaaaccaaa
      901 tattgcaatt actccaaaaa tacgctttaa tttcgcgcga cgcgcggatt cggggttggc
      961 atcagcttcg tttttaggaa tatttccctg tagtatctct gccataatct ataccttatt
     1021 caatatacat tgtagcattt tttagctatt ttgtcaattt ttgtgtgtgc tatatactat
     1081 ttattagcac tggtgtcttc gacggtgatt gaggtgtctt ggtgtaattc agtaagtgga
     1141 ttgtctatat agccacttgt tataaggccg tatatagcag ctacgccaat ggaaaaaaca
     1201 atgaacattc ttgttatttc ttcatgttgt cgtttttttt cttttgctgc acgtctttct
     1261 gctagtggca aagattgtgt attttcactc atttgatatg aaaactatag tacttttatt
     1321 atatggtgtc aagtggtgtg ctagaatggg cgatatgaca caaggtgagt ggattgccaa
     1381 cgcaacaaaa gcgttactga aatgcgctat aacaactgcg cggctcgatg ccctagtgct
     1441 aatgagttat gtaacaaaac aagacaaagc cgctattctt gcacatcccg aaactgccct
     1501 ttcgtcagcc gatatagcta cccttgctac agccctgaaa cgacgttgta atcacgagcc
     1561 gatagcatat attatgggca caaaagagtt ctatggacgt aattttttag tgactcccaa
     1621 cgtgctgata ccacgcccag aatctgaaga ttttattaat cttctaaaac actatccgcc
     1681 aacaaaaggc cacaaactca tagatatcgg tacaggcagc ggcattttag caattagtgc
     1741 aaaaaaagag ttccctgctt tgtctgtcta tgtaacagat gtatccaagg cggctttacg
     1801 cgtagctgct acaaatgcaa aaaaccatgc ggttaccata cagtgtattc aatcaaacct
     1861 gctacatacc gtaccgggtt gttttgacta tatttttgca aacttgccgt atgtaccaaa
     1921 agactattcc gtttcagccg aggtacttag cgaaccacaa cgggctattt ttgcggacaa
     1981 aaacggcttg gcgctgattt ttcgacttgc agaccaagcc aaaaaaagcc ttaccaaaga
     2041 cggcttgatt tttgttgaat ctcttattga acaacaagaa gaaatttgcg accactacca
     2101 aaaagccggc cttacagcta tagataatgc tgggttcatc caggttttta agaagtctgt
     2161 aatgagctaa agttctccac aaactactac atattaaata caaaatacta atttgtggat
     2221 ggggcgactt ctatggttgc tttggcagtt tgctctttac catcacggtt gtaggtaagt
     2281 tctatttcat cgccaacatt atatttgctt aatacagaag caagtggttt ttctatagtt
     2341 acttcttcgc catttacctt gaatataatg tcaccttctt ttaggccagc tttatctgct
     2401 ggacttccat ttaccaccgc ttgagcttcg ttaccagctt tcagccatgc accactactg
     2461 cgtgctaatt caaaacgttg ctgcagcgct tctgttaaca tgacataccg cacacccaag
     2521 tatggtactt cgagcttacc cttttctagt atgctggtta tttgcggctt tacgtcgttt
     2581 ataggaatag caaagccaat gttttctgca tcgccagcta ctgctgtgtt tatgccaatg
     2641 acttcgccac tcatgttaag cagtgggcca ccagagttgc ctgggtttat tgctgcatcg
     2701 gtttgaaaca aattagtgag tgattcggca cttgttgcgc tgccgtcgct ggctgttact
     2761 ggcctaccta gcccagaaat gatgccactg gttacagtgt tttggaactg accaagtgca
     2821 tagccaatag cgataacact atcgccaaca accatttggc tagaatcgcc gagtttaact
     2881 ggcttgagat ctttggcgcc atttacttta agaaacgcca agtctacatt agagcgtggg
     2941 tcacgagcta tcacgtctac attatcgtac tctgtacctt cgctggtcgt aatggctact
     3001 ttgctcgtgt tttctggtat gacgtgttta ttggtcacaa taacaccatc gctagaaata
     3061 ataaaacctg tcccggcact actttgggtt tgttcgccgt aaaaatacga attacccacc
     3121 gtactttcta catttataga aacaacactc gggctcacat ctttggcgat attgctaata
     3181 agttcactct gactggaggc aattttttgt tgcgttgctg tactgtctag ggatgtaatt
     3241 cggctggtgt ttaaacgact ccctaaaaag ccaccagcaa taccgctaat aaaggcgagt
     3301 gcaacgacaa taactgctac cctggttggt attgacgtag ttgatttagg acgcttgggg
     3361 gttggttcgc tttgttttac cggtgctggt gccatgacgg gtatacctct ctataattgt
     3421 tacaatttat attgtttttg cttgccaatc agaaaaagca atgataatta tcaataatat
     3481 agttataaac ccaataagct gatgttttac tgaatttttt aatttttcgt tactgtgcaa
     3541 gtagtacagc aacgctaaaa tatagccaac aagcgtaata atgagcgcaa cttgtggaac
     3601 ggtgacatat atgatgaccc agtgttctag cacccacgta agggtagcag cgaaccaacc
     3661 ccaaatagcg ctaagctgaa ttgtatatgg ttcttcaaaa gctccaaaaa agtgacgtgc
     3721 cgacaaatag cttatcagcc atactgctat cacgcctgtg gcaatggcgt ggtctggtga
     3781 tgcttgataa aaagcaatca gtgccataat ctgagcaagc agcgcctgaa tcataacaaa
     3841 tatttggcta gactttggct taagccacaa cagccacacc aagaagaata tcgtccatag
     3901 tagctgaacc aataaaattt tggtaccaga cataaaaacg acaatagata gtccaacaaa
     3961 aatatctacc gaattagcac gcaagtttgg cagccaatag cgtggacgca tggcaaatat
     4021 gcgccattta gaaagcagca ctaatgtaaa tgcaatgctt gcaaggtcta aacggacaaa
     4081 aactagtact aaaattggta gcgccgcgtt taatacaaaa tatagccacg aagaaacacc
     4141 tgattttggg cgtaagaatt gtaagtagtt tttggccata gacgctactt agcttacccg
     4201 ttttggccaa ttataacaat gtaatcagcg atgtaagggc ttatatctac accgtctgcc
     4261 ttgccaacgg cttgtgtatt aaaacgaagt tctaggtaac gttttgtgta ctttttagca
     4321 ccttttgtgg tgtctatcag ttgcgttaca ggtacattac tgactggtgc atctgctacc
     4381 tgtattacat tgtagccata gctttttagc tcatcggcac gcttagtggc agcaccagct
     4441 ttaccagatg cattcagcac aataatagta ggattttctt taataataaa accgtcttta
     4501 agttttaagc ggacaaattt ctttacttct gaataatcat caacacctgc aattgggtac
     4561 acaaccgatt ggccaccaat agaccccgtt gtaacaactg cttcgtcttt catggcgagg
     4621 tctacattgg taatgttttc gtttggtatt tttttagata gttcatacaa gcgcattaaa
     4681 tcatcagtag aaaagtttgt ttggactctg ttaccaaacg cgctaacgag ttgcgacact
     4741 ttaagtgggt tggcaaatgt gccggtgctg agggctttgt ctttaatggc aattaaaaca
     4801 agttgttgat tttcgcctcg gccaaagtcg tttctgcttt cttgcccctt aaacgaaccg
     4861 tatcgtgaac gcgccagccc aagagcatgg ccaccgtcta gctcattggg gccagctggt
     4921 aagttaagat actgcttacc tatcttcatg gttgggtcgc tataggcagc tggcaagtta
     4981 accgtaatgc ccccaagcgt gttaaccgat tcttcaaata ccgtaaagtc taccatggcg
     5041 tagtaattaa tattgacacc catataatct tgcacaacct ttttggttgc gttaatacct
     5101 gttttttctg cagcagattt atctttgggg ttcgtataga gtgcctgttc ttttgcactg
     5161 ttataaacgg cgttaatctt catgggccac aaatcgtctg gttttaccca taaatcgcgt
     5221 ggaatactca gcatcaccac gccatcattt ataggatcta tgctggcaat cataatagta
     5281 tcggttagct ggccaccttc gtgtttatcc ccgcccttac ccatcaagag cacgtttatt
     5341 cggccatccc cttcgccgtt taagctgttt gggtcaatat ctttagacat ggcgagcgcc
     5401 gacccaccac caccgagggc tttatgagcg tcccaccagg ctttgccaaa aataaaacca
     5461 aatatactta cccctgcaat gacaaaaaat agtgccattt ttgcatatgc ccgccgctta
     5521 cttttatgtt ttttcttacc tgatttattc tttgtaaata atcgcttgta gcgtggtgtg
     5581 tgcaatgact gagagctatc taatgtaaag ctttttctag aagtagcacg tgtatcatca
     5641 cctggtaatt ctatatgctc aaaatcacct gattcttgca tttggggggt gcgttgcccc
     5701 ctaaacccat cacgtcccga aaaattatct agctgattta agcggggtga ctttggtgtt
     5761 gcatttacag gtgttcgctt tggctgaaag ccttttcttg gtccggtaac caaaaaacca
     5821 tctgttgagt actgattttt tggcttgatt ttcttatatc tatccataat atgtacgtca
     5881 atacattata ccaaataatc ttttttttgg cacattgtaa cgcttttggt ttacaataat
     5941 gtagacgatg caaccaaacg agccacataa cccgaagagt tttgatatta agccatctac
     6001 gccgccatta ccacagccgc ctagtgagca tcctatcaca aaagagcaac ctgctcagtt
     6061 taacagtgcg cctcatatgg tgaacaataa cccagtgccg caaaatatcc agcaacctga
     6121 tcaagctgct caacttaagc aacgcaggcg tgaagagcgc caaaatatgc tgtacaccat
     6181 actgttattt atacttgctc ccctgtttgc ggtgttaatg atattatttg tctttcagag
     6241 ctatgtggtt gatggctcta gcatggaacc tacccttcaa aatgggaacc gtgtctttat
     6301 attaaagctc cctaaaacga tggcaaaatt acaaaacaaa gaatataccc ctgccagaaa
     6361 cgaagtaatt gtatttaaaa aacctaccaa cgatggcaca caacttatta agcgcgttat
     6421 tggcttgcca ggcgatagag ttgtgataga aaacggcaac gtcactatat ataacataga
     6481 taatcctggt gggttcaacc cagataaagg caccgaccac gaaaacaccc tagagccaat
     6541 agatagcgat ggccaaaaag tcgtcgaaga cgttggccaa ggtgaattat tcgtacttgg
     6601 tgataaccga ggccccggtg gctcacttga ttcccatagc gggcttggtt tggtaccaat
     6661 agataacata atagggagat tatgggtgcg ctacttcccc ttaagtgagt tccagacttt
     6721 ttcttctact tttgcaaatg ccgtattata cgatcaaact ctttttgaga agtatagcta
     6781 atagtaattt tccctttact gtttgacttt ggcactcgta ccgccaggcc aaggtgtttg
     6841 gtgagctgtt tagatagttg cgtacttgtt actgattttt ttgcatcgtc ttttgttttg
     6901 gtacgctcgg catctagctt tgcattcgct gctaaggctt ctgtctggcg gacagacaat
     6961 tttttggttt gaatcgcttt aaataacacc atttgagcat gtggatgttt tgctaaagac
     7021 aataaggccc tgccgtgacc ttctgatatg tcgcccttca ttagtgcttg ttgcattggt
     7081 tctggtaatt gcaataaccg tacagcatta acgacggttg tgtagccttt acctagccgt
     7141 agggcgatgt cttcgtagct ttgctggtat tcaacatgaa gccgttgaat tgttttggcg
     7201 gtttctatgg cgtttaaatc tgcacgttgt acattttcta gcagtgaaag ctctaagtgt
     7261 tgcagcgggt caacgtcttt tatgatagct gggacatgac gtagcccagc aagttgggcg
     7321 gcgcgccatc tgcgttcacc tgcaataatt gaatacaaat tatgttcaat ttctgcaaca
     7381 ataattggtt gtagtatccc ttgtgcttca atactcaaag ccagttgttg caattgttgt
     7441 tcatcaaaca gctgccgagg ttgattgctt tttggctgaa caatatctat ggcaagctta
     7501 tgtatttttt cattttttgg aatcgtcaca ccgtctatat tcgtaccttc tggtactaaa
     7561 ctatcaaagc ctcttcctaa ccctttttta gacacgttgc atcacctcct ttgcgagcga
     7621 tttatagctc cgcgctcctt ttgaccattt atcatacgta gcaattgatt taccatggct
     7681 gggtgcttct gccaaacgaa cattacgcgg aataactgtt ttacagacta aatcaccaaa
     7741 gactcgctct atttctgccc ttacctgcga acttaatgat gttctggtat cgtacatcgt
     7801 catgacgact cctacaatac taagcgtagg attaagcgtt tgttgaatac gctgaatggt
     7861 ttgcaccagt tggccaatgc cttccatggc gtagtattca gtttgcactg gtatcaatac
     7921 atagtctgca gcgaccaaac catttatact caaaagccct agtgatggtg ggcaatcgat
     7981 aatacatatg tcatattcgc tttcgatagc agacaaagct gtgctaagac ggtactctcg
     8041 tctggctatt gaaacaaggt ctacttctgc cccagaaagg ttatggttag acgtaataac
     8101 atgtaggttt ttctcaatag tagtatgtcg cacaatatcg gggctcgtat cattcaccag
     8161 tacgtcatat atgcttttgt ctgactcgta tttatctata cctaagccac tggtggcatt
     8221 accctgtgga tcaaggtcta ctaataagac gttttttccg ctacgcgcta tatatactgc
     8281 tacattaata gccgtggtag ttttaccgac cccgcctttt tggtttacaa cagcaattgt
     8341 tttcattttc tatacgtcac cttattctac cataagcgta ccacacctct agggttctcg
     8401 gctagtagtg gcgcaagtat ttagttgctg ttgttagctt cttctagggt gatacgaatt
     8461 ctatttatgg tcatgctgct tgcattgctg gtggctgtat cagaacacaa tgtgtagtac
     8521 acggcactgg ttgtatttgg gctatctagg aatatcattg tatttgaagc ctgttcacca
     8581 ttattggtag taaaagtact taggatggtt ccaacagggg taccactgcc acaggccgcc
     8641 ggcgatccaa tacttctttc tacccgtgca acaatagaac ggtcagcagt ggtggccgaa
     8701 gacacttcca tagatatgct accagttata gacttagccg tggtgcttgg tgaaaagtta
     8761 ggaaccgaat tattattctc accgctggta tcccagtatg atgttgtgtt agagtctact
     8821 gcagaatcag atgtcgtatc tataaagctc ctgacttgac gcgtaccaat acaatcaatc
     8881 cattcgccac cttcataaca tctaaactta cctgaagcgc tgttataata cataccacca
     8941 tccgtacctg ttggatcgcc agatgtattc ttagtatcaa gcactagtag cacaccgtta
     9001 gcatctgcac ttgcaccacc tatgcgtatg agcccaccgt tggtatcgac attaaagtag
     9061 tctacaccac tgccattttt aacagtgaaa gccgtgctac tggtaagtga tagctgtaaa
     9121 gcaccgctat tcaccattct tagcaccgac gttccattct tttgtaagtt gaggatgtcg
     9181 cctgatgcac ctgttttatt tatgtatagg ctattattgg ttgagttgtc ttgttgggca
     9241 ctccccgggg caattaatat aaagccacag gcgtcgctgc cgctaataca gaccgttccg
     9301 ctggcatctg gtaggtcaat attccttgat gcggtaagtg acgtaacgcc cagcacgctg
     9361 gtaaagccat tggcattaat gccatcgtgt aacagtaatc taccttgatt agacgatcca
     9421 gaaatatcac taccaagtga tagtaaacca tctgaactgg cggccactac tggcacgccg
     9481 ccgtctgttt ggacttcaaa aacgttggct aactggccag aaacagattg tagtactaaa
     9541 cctttttggt tgacaccatt ggtgtaaatt aagacttctg cagcggaatc tgctgtggca
     9601 gcagtattga cacttagcgt attagaagca gaagcattta taccaatttt attgcctgcg
     9661 gcgtcaaaga caagcgttcc attagcaata tctaggccgt ttggtagctg caaggtgtct
     9721 gagttaattg ttaaaacatc agtcgcagcg tcacctatag ttacatcacc gttcaagagt
     9781 gtatttgcgt tggctactaa attcccgcta attgtcgcga gacctgcaat ggtggtatca
     9841 ccgctcgctg aaactgttat acgcgtaaga ccaccagtaa caatttctaa atcattgaca
     9901 tctgacgtac caagtatggc tgctgcacca aaactattac caccctgtaa aaagccgctg
     9961 ccagggctac ctgcaggtgc ggcagttttt tcagctatcg tccatgaact gccggctaca
    10021 gcatattgcg ggccagacga accagattgc gaacata
//
LOCUS       DAOPLS010000005         9171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_7965_length_9171_cov_4.074375, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000005 DAOPLS010000000
VERSION     DAOPLS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7965_length_9171_cov_4.074375"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..387)
                     /locus_tag="PKV52_00245"
     CDS             complement(115..387)
                     /locus_tag="PKV52_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPD98675.1"
                     /translation="MSHVKAGGTSKNNHDSPGQRLGVKRFGGQTVRTGEVLVRQVGQT
                     KIAGEGTFIARNFTIHAAKDGVVHFMTRKVRTYTGKTVPRTEVIVK"
     gene            complement(408..1592)
                     /locus_tag="PKV52_00250"
     CDS             complement(408..1592)
                     /locus_tag="PKV52_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD98676.1"
                     /translation="MQYIAVLGRQPEFGLAELESLLGSQAITPIGGGVANIAYDHTIP
                     FARFGSVIKFAKVHQTIDTPSAHAVVSAFYKYIASEHPYEQGVKLKIGISDYSKTFSR
                     HTLGQLGLTLKKELKKEGYSVRIVPNKSPILGSAQVIHNNLTAPHGIEFVMFKQAGAL
                     HYATSVYEQDIEAYTARDQARPMRDARVGMLPPKLAQTIINLATGKTTDWQPAGHTVL
                     DPFCGTGVILQEALLMGYDVYGTDLEPRMIDYSQQNLLWLTERNAHIPKNIRLQVGDA
                     SSYTWQAPLDFIAAETYLGRPLTGLLQQSDLVDLVQSVNTLHKKVLINIHSQISANTR
                     LCLAVPAWRTKKGFAHLPILDQLDKMGYNRIEFTHAKAKDLIYIRDNQFVGRELVVLQ
                     KN"
     gene            1694..2761
                     /locus_tag="PKV52_00255"
     CDS             1694..2761
                     /locus_tag="PKV52_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPD98677.1"
                     /translation="MLSKRVKIIINAVTIIALIVLIYFSWPQITEGLKEIGGAKWSII
                     ALMIPLQVFNFFAVGMIYYSYFTSHRHSRVTKKNMFKVALELNFVNHVFPSGGVAGFT
                     YLGYRMKHYGVAVSRTTLAQSLRFALTFISFLILLFFGLFMLSFGDRSSGVTLYIGLS
                     IAFLTLFGTMLAVFIISDEGRIKAFTAFLPRLVNAVLKPFKKKRGNAIDLEKIEKLFG
                     DLHTDYKELMKDRKKLKKPFMWALLVNISEVLTIYLAYLALGQVVNPGAVILAYSVAS
                     FAGLVSILPGGIGVYEALMTTTLAASGVPKALALSATLIYRIFTMIIFVPTGFILYQL
                     ALRKGETEHISRDATVSTDTN"
     gene            2733..3857
                     /gene="xseA"
                     /locus_tag="PKV52_00260"
     CDS             2733..3857
                     /gene="xseA"
                     /locus_tag="PKV52_00260"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPD98678.1"
                     /translation="MQPLVLTPTDFIATTNQILETSYGFFYVEGELSQFRISKGKWVY
                     FDVIDEHAKVSFFGSVYMLPGPLTDGMIVRVGGTAKLHPQYGFSVTAQSIQPTGQGSI
                     TQAVALLAAKLQKEGLFATERKRLLPTIPTTVALVASVESAAYADFIKVTTARWPFAN
                     IEVYDTLVQGENAPDSLVRAVHAANTAPILADVLVMTRGGGSADDLAAFNDERVVRAL
                     SASRIPTMVAIGHEVDESLAELVADRRASTPSNAAELLFPDKAHELATVQAVRRSLAS
                     SASSFTAVEHSALRMQQQRLQRASETLLHAHQTMLAQQRQLLSSYNPSNVLARGYAIV
                     QSGAAVVQSAHLAVTKKQVHITFHDGTVAASVQKLIDNKE"
     gene            3862..4059
                     /gene="xseB"
                     /locus_tag="PKV52_00265"
     CDS             3862..4059
                     /gene="xseB"
                     /locus_tag="PKV52_00265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPD98679.1"
                     /translation="MPKKEFNYPAARQELNELLEWFESGAADLDKALANYKRAEELIS
                     QIESYLGDIEKKLHINVHKAE"
     gene            4059..4541
                     /locus_tag="PKV52_00270"
     CDS             4059..4541
                     /locus_tag="PKV52_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98680.1"
                     /translation="MFLIVIAAVILLFGFVVVIGAPYVPTLTQQQADALDLLALKKGQ
                     TVLDLGSGDGRFLRAAALSGYKAVGIEANPLLVLIAYVVCWRQRRNVTIIWGNMWQKQ
                     WPATDGIYVFLHSRFMQKLDNKVIQQYHGKNIKLVSYAFKIPSKKIVKKHSGMYLYHY
                     "
     gene            4646..5392
                     /locus_tag="PKV52_00275"
     CDS             4646..5392
                     /locus_tag="PKV52_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98681.1"
                     /translation="MPPNNQMPTAPNIFVPPAPGQVPNPMSPQLPPDPKTPVWLIVAM
                     IIATLLAIVFLVLTVLFYMQMSDYKNNSDQKSAAAVEQAKATQAEELKLQFTEQEKEP
                     LKSYTAPGSAASTKIVYPKTWSLYAVEGQNGNSVDAFFNPNIVPDTTNKGNVYALRMQ
                     VLDKPYATVVKEYDAPAKKGEVRISPYKPEQVSAAEQGVRIDGVVRDKVSGTMVILPV
                     RDKTIKIWTETENYVNDFNNFVLKNLEYSP"
     gene            5470..6141
                     /locus_tag="PKV52_00280"
     CDS             5470..6141
                     /locus_tag="PKV52_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98682.1"
                     /translation="MQIQTNTTHTATLLYELQQLLIQIKTCNELADDTSQATVGRLSQ
                     HGLQLLHYALFAIESQQTKLNFTSLSAGAAMHDVVEELLPLAASYGASIHFDASPNLD
                     PIYANEMSLKGSVYALLSAMITSSTNGVAPQITVAVQQTKPSVQRVGVYSDTTPISLA
                     MLRQTKQRTSRMDTTGLSHRSGLGFMISQLLAERLETAYASFEHNQLPGIGFYVPESA
                     QLQLL"
     gene            6145..6525
                     /locus_tag="PKV52_00285"
     CDS             6145..6525
                     /locus_tag="PKV52_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD98683.1"
                     /translation="MKILCIEPDVVLAKAFKTVFTRNHHDTIMLCLDPQAAIEQIDTQ
                     KPDAIVMELQLAPLSGLAFLHELRSYEDFSDIPVVVYSSVPQESFQADAATWRSLGVK
                     KYFYKSTDSLQKVARFIHGVLDEA"
     gene            6515..7198
                     /gene="recO"
                     /locus_tag="PKV52_00290"
     CDS             6515..7198
                     /gene="recO"
                     /locus_tag="PKV52_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPD98684.1"
                     /translation="MKPETTQAIVLTRTNYGEADKIVTSLTSHGKKISIIAKGVRKPK
                     SKLVAGTELFCVADICFVQGRGSMATLTSARLHSQHTRFLHDLNKVNLAYDVIKVVNK
                     HTEDSDGEYYFEILKQLFAALDNTSNDIDLVRIWAWMQMLHASGHQLRLDVQANGEQY
                     KEESAYDFDADSGGFVVSPNGNFAPTHIKLLRIAQTQPLSVLQRVNMAPTYAYELTPV
                     IKQFVENTY"
     gene            7215..7484
                     /locus_tag="PKV52_00295"
     CDS             7215..7484
                     /locus_tag="PKV52_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98685.1"
                     /translation="MSYKFKRISETKPNQMPDGLPRTINRVLGKFGIFLVDDEKPQIL
                     NIMAASEIGWAVVSFGNPKTGEITHQIEVPHGSRMSRDIEGSHDE"
     gene            7477..8817
                     /locus_tag="PKV52_00300"
     CDS             7477..8817
                     /locus_tag="PKV52_00300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPD98686.1"
                     /translation="MNKNEVQLEDIVSLAKRRGFVFQASEIYGGLAGFYDYGPLGTEL
                     ANKIKQEWWKAVVTNNDNIFGVDGAIIQNPKLWEASGHVAGFTDPLVDCKNCNARFRA
                     DHLADTDSTDLQELTLLLKDKKCPNCGKATLTEPRTYNMMMKTYIGPVEDSSAVAYLR
                     PETAGSIFTNFQNVRETMRAKIPFGIAQIGKAFRNEISPRDFIFRVRELEQMEMQFFI
                     NPHDQKDSYEQWRKFAWDFTTNKMGISDNNVAWHEHGEDERAHYAAAAHDIYFNFPFG
                     FKELWGTHNRTDYDLSNHARVSGKDLSYFDDVTKERYTPYVIESSVGVGRMFLAVLVN
                     AYQEDEQNGEKRIFLKLAEHLAPYRFAVSPLLKNKPELVAKAREVYEALKKKHGNVMW
                     DDNGNIGKRYRRQDEIGTPHCIVIDFETLENDTVTIRDRDTTQQNRVSIKELIE"
BASE COUNT         2799 a         1840 c         1999 g         2533 t
ORIGIN      
        1 ctgacagtat aattgatgca ttaattagtt ctgacatacc gctgagcgac accatagcat
       61 atacaaaaag agtccacgat gttatctgga ctccctatgg cgagtaagcc ataattattt
      121 aacgatgact tcggtacgag gaacagtttt accagtgtag gtacgtactt ttctggtcat
      181 aaagtgtaca acaccatctt tagcggcatg aatcgtaaaa ttacgtgcta taaatgtgcc
      241 ttcgccagca atttttgttt gaccaacttg gcgcacaagt acttcaccag tccgtacggt
      301 ttggccacca aagcgtttta caccaagccg ttggccggga ctatcgtggt tgtttttgct
      361 tgttccacca gctttaacgt gtgacatagg actccttacg ttttaggtta gttcttttgt
      421 aataccacga gctctcgacc tacaaattga ttatctctaa tatatatcaa gtctttagct
      481 ttagcgtgcg taaattctat acgattgtac cccattttat ctaattggtc aaggatgggc
      541 aggtgagcaa agcctttttt tgtacgccac gccggaacag caagacacaa gcgtgtgtta
      601 gcggaaattt gactatgaat attaatgagc acttttttgt gaagcgtatt tacactttgg
      661 actaaatcta caagatcaga ttgctgtagc aagcctgtaa gcgggcggcc aaggtacgtt
      721 tctgccgcaa taaaatctag tggtgcttgc catgtgtaag agctagcatc acctacctgc
      781 aaacgtatat tcttaggtat atgtgcgttg cgctctgtaa gccaaagaag gttttgctgg
      841 gaataatcaa tcattcgtgg ctctaggtcg gtcccgtaaa cgtcgtagcc cataagtaaa
      901 gcctcttgta atattactcc agtaccacaa aagggatcta gcactgtgtg gccagctggt
      961 tgccaatccg tcgtcttgcc cgtagccaag ttgataattg tttgcgccag cttggggggc
     1021 agcataccaa cccgagcatc gcgcatagga cgcgcctgat cacgagcagt atatgcttcg
     1081 atatcctgtt cgtacaccga tgttgcgtag tgcaatgcac cagcttgctt aaacatgaca
     1141 aattcaatac catgaggcgc cgttaaatta ttatgtatta cttgtgcgct cccaagtatt
     1201 ggtgatttat taggcacaat acgtactgaa taaccttctt ttttaagttc tttttttaag
     1261 gtaagcccaa gctggcctag cgtatggcga gaaaacgttt ttgaataatc actgatgcct
     1321 attttaagct ttactccttg ttcgtatggg tgctcgcttg caatatattt ataaaaagcg
     1381 ctcaccactg catgagcaga gggcgtatca atagtttggt gtacttttgc aaatttaata
     1441 acagaaccaa aacgtgcaaa gggaatggta tgatcgtagg caatattcgc tacaccaccc
     1501 ccaattggag taatagcctg cgaaccgagc aaactttcta gttctgccaa accaaattct
     1561 ggttgtctac ctaacacagc aatatattgc atactcggtc tcattataac gtactgtacc
     1621 gtattagtat gtacactatc tccagcttat ataggggtta cattatgttc atctgatata
     1681 ataaacagcg tctatgcttt ctaagcgcgt aaaaataatt atcaatgccg ttacaattat
     1741 tgctcttatt gtgctaattt acttttcttg gccacaaatt accgaaggcc tcaaagaaat
     1801 aggtggggca aagtggtcta ttattgcgct aatgataccg ctgcaagtat ttaacttttt
     1861 tgcagttggc atgatttatt acagctattt tacaagtcat cgccatagcc gtgtcacaaa
     1921 aaagaatatg tttaaggtag cgctagagct taactttgta aaccatgtgt tcccaagtgg
     1981 tggggtggct gggtttacgt atttggggta cagaatgaag cactatggtg tggcggtatc
     2041 acgcacaaca cttgcacagt cattacgctt tgccttaacc tttataagtt ttctgatact
     2101 actctttttt gggttattta tgctaagctt tggtgatcgc agtagtggcg taacattata
     2161 tattggttta tcaattgcat ttttgacttt atttggcacg atgctcgccg tgtttatcat
     2221 tagtgatgaa ggccgtataa aagcctttac agcttttttg ccacggttag taaatgcggt
     2281 cctaaagcca ttcaaaaaaa agcgtggcaa tgccatagac ctagaaaaaa tcgaaaagct
     2341 gtttggcgat ctgcacaccg attacaaaga acttatgaag gacagaaaaa agctaaaaaa
     2401 gccctttatg tgggccttgc ttgtaaatat ttctgaggtg ctcaccatat atttagcata
     2461 tttggcctta gggcaagtgg tgaatcctgg cgcggtgata ctggcgtatt cggttgcttc
     2521 ttttgctggt ttggtatcta tactacccgg tggtataggt gtatacgagg cgcttatgac
     2581 cactacatta gccgcatctg gcgtaccaaa ggcgttagcg ctttctgcaa cactaatata
     2641 tcgaattttc accatgatta tttttgtacc aactggcttt attttgtacc aactggcact
     2701 aagaaaaggt gaaacggaac atatatctcg cgatgcaacc gttagtactg acaccaactg
     2761 attttatcgc gacaaccaac cagattctag aaacgagtta tggctttttt tatgtcgaag
     2821 gtgagctcag ccaatttaga ataagcaaag gcaagtgggt gtattttgat gtcatagacg
     2881 aacacgctaa agtgtcattt tttggatctg tctatatgct gccagggcca ttgacagatg
     2941 gcatgattgt ccgggtggga ggtacggcca aactacaccc gcaatacggt tttagtgtaa
     3001 cggcacaaag tattcaacca acagggcaag gatctatcac ccaagcagtg gcacttttgg
     3061 ccgccaagct acaaaaagaa ggcttatttg ctactgaacg gaaacggctt ttacctacga
     3121 tacctactac cgtggcgctt gtagctagtg tagaatcggc tgcgtatgca gattttatta
     3181 aagtaacaac tgctcgttgg ccatttgcta acatagaagt atacgatacg cttgtgcagg
     3241 gagaaaatgc tccagatagt ttggtgcgcg ccgtgcacgc tgccaacaca gcgccgatac
     3301 tggcggacgt gttggtgatg acgcgcggtg gtggtagcgc ggatgattta gcggcattca
     3361 atgatgagcg tgtggtgcga gcgctatctg ccagcagaat acctactatg gttgcaatag
     3421 gccatgaagt tgatgaatca ctggcagaac tcgtcgcaga taggcgcgcg agcacaccaa
     3481 gtaatgctgc agaactatta tttcctgata aagcgcatga acttgcaacg gtgcaggctg
     3541 ttcggcgcag ccttgcttct tctgcatctt ctttcactgc agtggaacat agcgccttac
     3601 gcatgcaaca gcagcgcttg caacgtgcta gtgaaacgtt attgcatgcg caccaaacaa
     3661 tgcttgccca acaacgtcag ttactaagtt cgtataaccc gagtaatgtt ttggcaagag
     3721 ggtatgcaat tgtacagagt ggtgccgcag tggtgcaatc tgcgcatctg gcagtcacta
     3781 aaaaacaagt acacataacg tttcatgatg gaactgttgc cgcaagcgta caaaaactaa
     3841 ttgataataa ggagtaatgt tatgccaaaa aaagaattta attacccagc tgcacgccaa
     3901 gaacttaatg aacttttaga atggtttgaa tctggcgcag ccgacttaga caaagcactg
     3961 gctaactaca agcgggcaga agagctgata tcccagatag aatcatactt aggtgacata
     4021 gaaaaaaagc ttcacattaa cgtacataaa gcagaataat gtttcttatt gttattgcgg
     4081 cagtaatctt actatttggg tttgtcgttg ttataggcgc accgtacgtg cctacactga
     4141 cacaacagca agccgatgcg cttgatttac ttgcgcttaa gaaaggccaa acagtattgg
     4201 atctagggag tggagatggt agatttttac gggcggcagc tttaagtggg tataaagcag
     4261 ttggtatcga ggccaacccg ctactcgttc ttattgccta tgtagtgtgt tggcggcagc
     4321 gccgtaacgt aacaattata tggggtaata tgtggcaaaa acagtggcca gctactgacg
     4381 gtatctatgt ttttttacat agcagattta tgcaaaagct ggataataaa gttattcaac
     4441 aatatcatgg taaaaatatc aaacttgtga gctacgcctt taaaataccg agtaaaaaaa
     4501 tagttaaaaa acacagtggc atgtacttat accactacta agatgtgtaa agggtgtaag
     4561 aaaaacatat aattatggaa tacctctacc ttaacaaaac ctacgcatct accatctatt
     4621 gctaaaatct ggtagcataa gagggatgcc accgaacaat caaatgccaa ctgcaccaaa
     4681 catttttgtg ccacctgctc ctgggcaggt accaaaccca atgtcaccgc agttgccacc
     4741 agatcctaaa acaccagttt ggcttattgt tgctatgata attgctacgt tgttagctat
     4801 tgtttttttg gtgcttacag tgctgtttta tatgcaaatg tcagattaca agaacaatag
     4861 tgatcaaaaa tctgctgctg cagtagaaca agcaaaagca acgcaagcag aagagctgaa
     4921 gttacaattt actgaacaag aaaaagaacc gcttaaaagt tacactgcac ctggatctgc
     4981 cgcaagcacc aaaattgtgt acccaaaaac atggagctta tatgccgtag aagggcaaaa
     5041 tggcaatagt gtagacgctt tctttaatcc gaatatcgta ccagatacaa ctaacaaggg
     5101 aaatgtttat gcattgcgta tgcaagtgct cgataagccg tatgctacgg tggtaaaaga
     5161 atatgatgca ccagccaaaa agggtgaagt acgtatttct ccctataaac cagaacaagt
     5221 tagcgctgcc gaacagggtg ttcgtataga cggtgttgtg cgcgataaag taagtggcac
     5281 catggttata ctgcccgtac gtgataaaac tatcaaaatt tggacagaga cagaaaatta
     5341 cgttaacgac tttaataatt ttgtgttaaa aaacctcgaa tacagtccgt aatttctgct
     5401 atacttagta catagtaacg gtgaaatcca tctggtggag acggtttaag taaccgaacg
     5461 taagagggta tgcagataca aactaataca acacacaccg caacgctttt atatgagctt
     5521 cagcaattgc tcatacagat aaaaacatgc aatgaacttg ccgatgacac gtcacaggca
     5581 acagtgggtc gtctatcgca acacggctta caattgctgc actatgcatt atttgctata
     5641 gaatctcagc agacaaaact taattttaca tctttgagcg caggggctgc gatgcatgat
     5701 gttgttgaag aattattacc acttgctgcg tcttatgggg cgtctattca ctttgatgca
     5761 tcgcctaacc ttgaccccat ttatgccaat gaaatgtcac ttaaagggag tgtgtatgca
     5821 ctgctgagcg caatgataac aagtagtacc aatggtgttg caccacaaat taccgttgcg
     5881 gtacaacaaa cgaaaccttc tgtgcagcgc gtaggggtgt attctgacac aacaccaatt
     5941 agccttgcaa tgctccgcca aacgaagcag cgtaccagtc gcatggatac aactggcttg
     6001 tcgcacagaa gtgggctagg ctttatgatt tcgcagctgc ttgcagaacg actagaaact
     6061 gcatacgcat cttttgaaca caatcaactg cccggtattg gtttttatgt gccagaatct
     6121 gctcagctgc aattgctata atctatgaaa atattatgca tagaaccaga tgtagtactg
     6181 gcgaaagcct ttaaaaccgt ctttacgcgg aaccaccacg ataccataat gctgtgtttg
     6241 gacccgcagg ctgccataga acaaatcgac acgcaaaaac cagatgcaat tgttatggag
     6301 ctacaacttg caccactgag tggcctagca ttcttgcacg aactacgtag ttatgaagat
     6361 tttagtgata ttcctgtggt agtgtatagc agtgtgccac aggaatcgtt tcaggcagat
     6421 gcagcgacgt ggcggtcatt aggcgtaaaa aagtactttt ataaatcgac agattcgcta
     6481 caaaaagtgg cgcgttttat acatggggtt ttagatgaag cctgaaacta cacaagctat
     6541 tgtgcttacc agaacaaact atggcgaagc cgacaaaatt gttaccagtt taacgagcca
     6601 tggtaaaaag ataagtatta ttgctaaggg tgtacgtaaa ccaaagtcaa aacttgtagc
     6661 tggcacagaa cttttctgtg ttgctgatat atgttttgta caagggaggg gtagcatggc
     6721 aacactaacg agtgctcgct tgcattcaca acacaccagg tttttacatg atctaaataa
     6781 agtaaacctt gcctatgatg ttataaaggt ggtgaataaa catacagaag acagtgatgg
     6841 ggaatattat tttgaaattt taaaacaact ttttgctgct ttagataata catcgaacga
     6901 tattgaccta gtgcgaatat gggcgtggat gcaaatgcta cacgcaagcg gtcaccaact
     6961 tcgccttgac gtacaagcta acggcgaaca gtacaaagaa gaatctgctt acgattttga
     7021 cgctgacagt ggtggttttg tcgtatcacc taatggtaat tttgctccaa cacacataaa
     7081 attactacgc attgcccaaa cacagccact gagtgtattg caacgagtta acatggcacc
     7141 aacctatgcc tatgagctaa cgccggttat aaagcagttt gtagaaaaca cctattaata
     7201 gatatactac ctgtatgagt tataagttta aaagaattag tgaaaccaaa cctaaccaaa
     7261 tgccagacgg tttaccacgt accataaata gggttttagg caaattcggc atttttctgg
     7321 tagatgatga aaaaccgcaa attttaaata ttatggcagc atcagagatt ggatgggctg
     7381 tagtaagctt tggcaatccc aaaacaggtg agataacaca tcaaatagaa gtacctcacg
     7441 gatcacgaat gtcgcgtgat attgaaggtt cgcatgatga ataaaaatga agtacagcta
     7501 gaagatattg taagtttggc caagcgccgt ggctttgtgt tccaggcgag tgagatctat
     7561 ggcgggctag ctgggttcta tgattatggc cctttaggaa cagagctcgc taataaaata
     7621 aaacaagagt ggtggaaagc agtcgttacc aataacgata atatatttgg cgtagatggt
     7681 gcgataattc aaaaccccaa gctgtgggaa gctagtgggc atgttgcagg ctttaccgac
     7741 ccactggtag attgcaaaaa ttgtaacgcc cggtttaggg cagatcattt agcagatacc
     7801 gattctactg atttgcaaga actaaccttg ttactcaaag ataaaaaatg cccaaactgt
     7861 ggcaaagcca ccctaaccga accacgcacc tataatatga tgatgaaaac ctacataggc
     7921 ccagtagaag atagttcggc cgtggcttat ttacggccag aaaccgctgg ctcaatattt
     7981 acaaattttc agaacgtgcg cgaaacaatg cgtgccaaaa ttccattcgg tatcgcacaa
     8041 ataggaaaag cgtttcgtaa cgaaattagc ccacgtgatt ttatttttag ggtgcgtgag
     8101 ctagaacaaa tggaaatgca gttttttatt aatcctcatg atcaaaaaga tagctatgaa
     8161 cagtggcgca agtttgcttg ggactttaca acaaacaaaa tgggaatttc tgataataat
     8221 gtagcttggc acgaacacgg tgaagacgaa agagcacatt atgccgcggc agctcatgac
     8281 atttacttta attttccgtt tggctttaaa gaactatggg gcacgcataa tcgtacggat
     8341 tatgatttaa gcaaccatgc acgggtgagt ggtaaagatt taagctactt tgacgatgta
     8401 actaaagaac gttacacacc atatgttatt gaatcatcgg taggtgtggg aagaatgttc
     8461 ttagcagttt tggttaatgc ataccaagaa gatgaacaaa atggtgaaaa acgtatattc
     8521 ttaaagctcg cagagcacct agcaccttac aggtttgcag taagtccact attaaaaaac
     8581 aagccagagc ttgttgctaa agctcgcgag gtgtacgagg cattaaaaaa gaagcacggt
     8641 aacgtaatgt gggacgataa cggaaatatc ggtaaacgtt atcgccgcca agacgaaatc
     8701 ggcacaccac attgcatagt gatagatttt gaaacattag aaaacgatac cgttacaata
     8761 cgcgaccgcg acaccacaca gcaaaacaga gtttctatta aagagcttat agagtaatct
     8821 tacttatctg attgcaactt ttaccgctac aagttgcttg aaaagcgctg ggcgaaccta
     8881 caaaacatgt atatttagga gtatttcttt cttaaattaa ctagcttcca tctttggcat
     8941 ccagaatata atgtactttt tagctggtag aaaaagtaaa aaataactgt tattgccaat
     9001 aaagtgtcat taattaaact attaattacc caaaatacac gtagctgtgc atgtgtgtat
     9061 aaatttcggc tgtgctagta ggtcttttat tacccgtggt actatagtaa aaatgaatgt
     9121 attgcataaa gacattacga aacgtatata caaagacgct aaagacgtat g
//
LOCUS       DAOPLS010000006         8954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_8227_length_8954_cov_5.075739, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000006 DAOPLS010000000
VERSION     DAOPLS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8227_length_8954_cov_5.075739"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..304
                     /locus_tag="PKV52_00305"
     CDS             2..304
                     /locus_tag="PKV52_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPD98687.1"
                     /translation="MPGAVLDYPFGKGVAVYKVGADEAARQKMFALIAEDTTPLRLSL
                     KCDPQLAVVLREKYETVLPGYHLNKKHWNTIILTGQLTWEEVQDLIRHSYNLVVSG"
     gene            complement(385..960)
                     /locus_tag="PKV52_00310"
     CDS             complement(385..960)
                     /locus_tag="PKV52_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98688.1"
                     /translation="MDQNNYEFIMNQTSQKKPLFGGSKKQRIIIIVAGAALALLLGIL
                     LVTVISASSKNTQEVLAPVGGAQADIIAITEIGAKEARDTATINKTASVSLTVSSQNA
                     AIMGQLGKNAKKIIAPYQNSGYEAVLDDAKNSGSFDKTYQTILTERLDLYRQTLVTAY
                     SQTKSAKLKKELESYYAQIGVILGEPTTPKQ"
     gene            1090..2847
                     /gene="dnaG"
                     /locus_tag="PKV52_00315"
     CDS             1090..2847
                     /gene="dnaG"
                     /locus_tag="PKV52_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPD98689.1"
                     /translation="MDAVEEVKQRLSIEDVVAEYVELKRSGRNFKGLSPFTSERTPSF
                     IVSPEKQIWHDFSSGRGGDMFSFIMEMEGVDFRGSLEILARRAGVDLSLYDKGDGGGM
                     RKKKDAAQAALTMAVRYYQQSLLHSPSALAYVTDTRALRRQTIADFGIGYAPNTGTAL
                     VDALTKKGVSVHDMKLAGLANDRYGKVNDMFRGRIVIPLYDAQGNPLGFTARILHKED
                     SGPKYINTPQTILYDKSRHVFGLHAAKEAIRTSGFVVIVEGNMDVISTHQAGYKNVVA
                     TAGTAMTGFQIKALQRLTTDIRLCFDQDAAGVAAAERAIDVAQAAGVQLSIVSLPEGK
                     DPDELIQKNVALWEEAIHSPVYAVDWLIDRYKEQFDITSASGKRKFSDALAHIVNSLH
                     DSVEKDHYIKVLAEIAGVSESAIKTKLAVMASGEKASLKKRKNDAPTLGHDQEAYQDQ
                     LLSLLVMFPITRRFLETADTTYFVFSTPERQRVFEYVSQNPHVTITDSIPDDLKDVED
                     YVKILLLKAEELYAVGFDANERLRELQDLIEKLKSKYFVSQRQLLSAQIKQAEEAGDE
                     EKVNTLLEQFNQLLKKGQS"
     gene            2849..3961
                     /gene="rpoD"
                     /locus_tag="PKV52_00320"
     CDS             2849..3961
                     /gene="rpoD"
                     /locus_tag="PKV52_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPD98690.1"
                     /translation="MVDKPADLTDKKSKLLEKARISGKIEYKEILAMIPEAADNIELY
                     DTISSELVDAGIEVNGHEDPPLEVIAKVEDEWEAEDEEEIMTTQAALLDDVSDDSVRL
                     YLREIGKIPLLSSEEELALAHKVVAGDKRAKDKMAEANMRLVVSIAKRYVGRGLDLLD
                     LIQEGNTGLLRAVEKFDPDKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLLRTQRRLTQELNREPTNEEIAKEMEMEVEKVEHIMKIKQDIHSLDQSVRDDEEDSV
                     LGDFVEDEDTVTPEESATNQLLKEHVQELLSGLSEREQKIIRMRFGLDDGKNHTLEEV
                     GHEFSVTRERIRQIEAKALAKLRKHKESKRLLDYIR"
     gene            3973..4533
                     /locus_tag="PKV52_00325"
     CDS             3973..4533
                     /locus_tag="PKV52_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD98691.1"
                     /translation="MIIGLSGTLSSGKDTLADHLQEKFGLMHISTGDIVREIAQSQRG
                     SVERPVLYDVGNELRHSYGGGVLVDRALDRYQNSIRTYAGAVITGIRSLAEAKAIKEK
                     GGTIVFVNAPLELRFSRMQSRHRDGEARITLEAFKQREQKELASGLSDADFNITQIGQ
                     IADIHLQNNGSLEDFFAAAERELQLA"
     gene            4555..5667
                     /locus_tag="PKV52_00330"
     CDS             4555..5667
                     /locus_tag="PKV52_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPD98692.1"
                     /translation="MPKPTDTIETTDFTLFKQQLSTHKHTIDTELQRISEHLLADTNE
                     QFGEVPKEVVAAYAAVLLKGGKRIRGSLAMAAYALFEGDNQPMITQAACALEMLNTYI
                     LIADDIQDRSESRRGGKTAHIQMRDFHVKKHLKGDSLHFGEAMAINSFLIAQHYAANL
                     IATLPTSADIRIKALQNVNKCFIVTAHGQTMDIFVESNGAASSEEVNHILEWKTAYYT
                     FINPLQLGGILAGARKADMAKLADYGLHAGRAFQLVDDIIGTFGSSDMTGKSPLDDIK
                     EGKRTLMVLAALEKANASDSYFLEACLGSQQLTMHDFEKCQSIITKTGALDYTKEQAE
                     QSSKKAIAAISSETTWVPQAKQFLIDLAQYILVRNT"
     gene            5705..7177
                     /gene="crtI"
                     /locus_tag="PKV52_00335"
     CDS             5705..7177
                     /gene="crtI"
                     /locus_tag="PKV52_00335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPD98693.1"
                     /translation="MKKKIAIIGAGFGGISAAAYLARDGYDVTVFEKNSQPGGRAIVY
                     KHKGFTFDMGPSWYMMPDVFEDFFTDFNTSSEKYYQLKKLLPSYRVYNNTTSHDVSPV
                     DEGGLAVFDDLEPGAAKQVKQLLKKTGKEYRHIREHLLEKDYISPIDAIDSKALSMLA
                     NPELFFSYHSRIKKTSRNQDIQKILEFMTVFMGGSPQNIPGVYSLLSHVDLSLGIWYP
                     MGGFGAVVKGFESIAKQQGATFVYNAEVTRIHTTQHAVSHVEVAGVKHQFDAVIANAD
                     YHHVETKLVPRNYQSYPEKYWAKKTLSPSAMLVYLGVDKVVPGLLHHTMFFDADWHGH
                     FDQVFSKKEWSLRPLFYVSNPSKTDPTVAPKGKENIIMLAPLANGLHPTKDQINELVE
                     SLIARLEARTKTNISKHIVVKKVVLMDFFAETFNAYKGNAFGLAHTLGQSAMFRPRMQ
                     SKKLKNLYYVGQYTNPGTGVPMVVLSGKIIARVVGERVRQ"
     gene            7174..8016
                     /locus_tag="PKV52_00340"
     CDS             7174..8016
                     /locus_tag="PKV52_00340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HPD98694.1"
                     /translation="MTREKEIFKQGSTTYYWSSVFFPEQTKEDVFKLYSFVRIADNFV
                     DAKPQNKKSFIQLKKAWTALVNINKQPSKKMNSDIRLAVTNMYDVYKKYDFSKEWVSA
                     FLDAMEQDLGKKRYKTLDDIIAYMYGSAEVIGLMMAKIIGVPERGYEAARMQGRAMQY
                     INFIRDIAEDNQLGRAYFPANELKKYGLPSVSKEDVYRHPAAFREFIEAQLAYYTKWQ
                     KQADKGMKYIPRSQRIALRTAVDMYNWTAVAIAKNPFIVFDKKIKPSKSRVVVRALVR
                     SIHA"
     gene            8009..8692
                     /locus_tag="PKV52_00345"
     CDS             8009..8692
                     /locus_tag="PKV52_00345"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HPD98695.1"
                     /translation="MREFTYYLFNLLVFLPVLVLSFTTDVKPHRHVRGLLAGFLLVSV
                     PFMLWDIWAAQAGHWGFNVAYITGPYLFGVPLEEYLFFITVPFAMIYVWGVVKKYVTD
                     RATAGFFPLLAFGITAGYSVWLLVNYWGNGYTRSVAIAAIIAVLVAAVSRIAYTWRFW
                     TFQVVLLGLFLVFNSLLTMLPIITYGPDAIIGFKIGDIPIEDFLFNFAFINLFLLVYT
                     FVDQPRIVK"
     gene            8745..>8954
                     /locus_tag="PKV52_00350"
     CDS             8745..>8954
                     /locus_tag="PKV52_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98696.1"
                     /translation="MKPSPEAHQYQNVTDTISYFGNTYASWPVYTQVDQLLMASIGVG
                     GSIAPEELLGVINSKECSLSAKIFAV"
BASE COUNT         2784 a         1687 c         2063 g         2420 t
ORIGIN      
        1 catgcctggt gcagtgctcg attacccatt tggcaaaggt gtagcggtat ataaagttgg
       61 tgccgacgaa gcagcgcggc aaaagatgtt tgcgctgatt gcagaagaca ccacaccctt
      121 acgattgagc ttaaaatgcg acccacaact tgcggttgta ctccgcgaaa agtatgaaac
      181 ggtattgccg ggctatcact taaacaaaaa gcactggaac acgattattt taactggtca
      241 gcttacatgg gaagaagtgc aagaccttat tcgccatagc tacaatttag tagtaagtgg
      301 gtaagtatag aagagagtaa gctgctaagt attaagatac agcagcactc tactcactta
      361 ctcacttata aacttttcac cgctttactg ctttggggtg gtgggttcgc ccaagataac
      421 accaatttgg gcatagtacg attcgagctc ttttttaagc ttggcagatt ttgtttgact
      481 ataggcagtt acaagggttt ggcggtataa atctagccgt tcagtaagaa tggtttggta
      541 ggttttgtca aacgaaccag aatttttggc atcatctagc actgcttcat agccactatt
      601 ttggtatggg gcgattattt ttttggcatt tttgccgagt tgccccatga tagcggcatt
      661 ttgtgatgat acggttaagc taacagaggc agttttatta atagtggctg tgtctcgtgc
      721 ttctttggcg cctatttctg taattgcgat aatatcagct tgggcaccac caaccggcgc
      781 tagtacttct tgcgtatttt tactactggc agatatcacc gtgaccagta atatgccaag
      841 caataacgct agtgctgctc cggccacaat aataatgatg cgttgttttt tactaccacc
      901 aaacaatggt tttttttgcg atgtttggtt catgataaat tcgtagttgt tttggtccat
      961 tactatatga ttatagatga aacttttggt aatgtggaat aaaagcataa atagtatagc
     1021 gcacagtggg ctacgagtaa ggcgcgacac ggtctttacg ctaggcaata aacgctatac
     1081 ccttatagca tggatgctgt agaagaggtt aagcaacgtt taagtataga agatgtggtt
     1141 gctgaatatg ttgagctaaa acgttctggg cgcaatttta aaggtctgag tccgtttaca
     1201 tctgagcgta cacccagttt tatcgttagc ccagaaaaac aaatttggca tgattttagc
     1261 tctggcaggg gtggtgatat gtttagcttt attatggaaa tggaaggcgt agactttagg
     1321 ggttcgctag aaatattggc tcggcgtgct ggtgtagacc tgagtttgta cgataaaggc
     1381 gatggtggtg gtatgcgtaa gaaaaaagat gctgcccaag ctgctttgac tatggcggta
     1441 cggtattacc aacaatcgct cttgcatagc ccatcggcac tcgcctacgt aaccgacaca
     1501 cgggcgttac gccgccaaac tatcgccgac tttggcattg gctacgcacc aaacactggt
     1561 acagcacttg tggatgcact gaccaaaaaa ggtgtgtctg ttcatgacat gaaacttgct
     1621 gggcttgcga atgatcgcta cggcaaagtg aatgatatgt ttcggggtag aattgttatt
     1681 ccgctatatg atgcccaagg gaacccactt ggttttacag cacgtatttt acataaagaa
     1741 gacagtggtc ctaaatacat taatactcca cagacaattt tatatgacaa aagtcgccac
     1801 gtctttggct tacacgcagc caaagaagct atcagaacga gtggttttgt ggtgattgta
     1861 gaaggcaata tggatgtgat tagcacgcac caagcaggct ataaaaacgt tgtcgcgacg
     1921 gccggtactg caatgacggg ttttcagatt aaagccttac agcggttgac gacggatatt
     1981 cggctttgct ttgaccaaga cgcagcaggt gttgctgcag ccgaacgcgc catagatgtt
     2041 gctcaggctg caggtgtaca actttctatt gtttcgctcc cagaaggtaa agatcctgac
     2101 gagctcattc aaaaaaatgt tgcgctatgg gaagaagcga ttcattcgcc tgtttacgca
     2161 gtagattggc tgattgatag gtacaaagag cagtttgata ttaccagcgc tagtggcaaa
     2221 cgaaagttta gtgatgcctt ggcgcatatt gtgaatagtt tgcacgattc tgtagagaaa
     2281 gatcactaca ttaaagtatt agcagaaatt gcaggtgtga gtgaatctgc cataaaaacc
     2341 aagttagcgg ttatggcatc tggcgaaaaa gccagcctaa aaaagcgcaa aaacgatgcg
     2401 cctacgctcg gccacgacca agaagcatac caagatcaac tgctatcact actagttatg
     2461 ttcccaataa cgcggcggtt tttagaaacg gcagatacta cctattttgt gtttagcacc
     2521 ccagagcgcc aacgcgtatt tgagtatgtg agccaaaatc ctcacgttac aattacagat
     2581 tctatacctg atgatttgaa agatgtcgaa gactatgtca aaatactact actgaaagca
     2641 gaagaactct acgcagttgg ctttgatgcc aacgaaagat tgcgagagct gcaagattta
     2701 attgaaaaac taaaatcaaa atattttgtg agccaaagac aacttttgtc tgcgcagatt
     2761 aagcaggcag aagaggctgg cgacgaagaa aaagtaaaca cattgctaga acaattcaat
     2821 caactattaa agaaagggca atcatagagt ggtagataaa cctgcagatt tgactgacaa
     2881 aaaaagtaaa ttattagaaa aagcgcgtat aagcggcaaa atagaatata aagaaatatt
     2941 ggccatgatt ccagaagcag ccgataatat tgagctatac gacaccatta gctctgaatt
     3001 agtagacgct ggtattgaag taaatgggca tgaagatcca ccactagaag taatcgccaa
     3061 ggtagaagac gagtgggagg cagaagacga agaagaaatt atgactaccc aagcagcgtt
     3121 gcttgacgat gtttctgatg attcggtgcg cctatatttg cgtgaaatag gtaaaatacc
     3181 gctactttcg tctgaagaag agcttgcttt ggcccataaa gtggtggcgg gcgataaacg
     3241 cgctaaagac aaaatggcag aggccaatat gcgccttgtt gtttctatag ccaagcgata
     3301 tgttggccgt gggttagatt tattagactt aattcaagaa ggtaatacgg ggttgttgcg
     3361 tgcggtagaa aagtttgacc cagacaaagg ctttaaattt agtacgtatg caacgtggtg
     3421 gattcgccaa gcaattaccc gagcgattgc tgaccaagca cgaacgattc gtattcctgt
     3481 acacatggta gaaacgatta ataagttact tcgtactcag cgccgtttga cgcaagaact
     3541 taaccgcgaa ccaaccaacg aagaaattgc caaagaaatg gaaatggaag ttgaaaaagt
     3601 tgagcatatc atgaagatta aacaagatat tcactcactt gatcaatcgg tacgtgatga
     3661 tgaagaagat tctgtacttg gtgacttcgt agaagacgaa gacacagtaa cgccagaaga
     3721 atcagctacg aatcagctat taaaagagca cgtgcaagaa cttctaagcg gcctaagtga
     3781 gcgtgaacaa aaaattatcc gtatgcgatt tgggcttgac gatggcaaaa accatacact
     3841 agaagaagta ggtcacgaat ttagtgttac ccgtgaacgt attcgccaaa tagaagctaa
     3901 agcgttggca aaacttcgta aacataaaga aagtaagcga ttactcgact atatacggta
     3961 atataaaagc atatgattat aggattatct ggaacgctgt ctagtggtaa agacacactt
     4021 gccgatcacc tgcaagaaaa atttggtcta atgcatataa gtacgggtga tattgtgcga
     4081 gaaatcgctc agtcacagcg cggttcggta gaacgccccg tgctatatga tgtaggcaac
     4141 gaattacgtc actcttacgg tggcggagtg ctagtagata gggcactaga caggtaccaa
     4201 aatagtattc gtacctatgc aggtgccgtc attactggaa tacgcagttt ggctgaggcc
     4261 aaagccataa aagaaaaggg gggcacgata gtatttgtaa atgcccctct agaattgcgt
     4321 tttagccgta tgcaatcacg ccatcgtgac ggcgaagcgc gcataacgtt agaagctttt
     4381 aagcagcgtg aacaaaaaga acttgcaagt ggcctgagtg atgccgattt taatattaca
     4441 caaattggcc agatagccga tatacattta caaaacaacg ggtcattaga agactttttt
     4501 gcagctgcag aaagggagtt gcagctggcc taggaaagac aagcaggtag tatcatgcca
     4561 aagccaacag atacaattga gaccacagat tttacacttt ttaagcaaca actaagcacc
     4621 cataaacata ctattgatac agagctgcag cgcataagtg agcatctgct tgcagataca
     4681 aacgaacagt ttggtgaagt accaaaagaa gtggtcgcag cgtacgcggc tgtactactg
     4741 aagggtggta agcgtattcg tggctcactg gcaatggcgg catatgcttt gtttgaaggc
     4801 gataatcaac caatgattac ccaagcagcg tgcgctctag aaatgctcaa cacctatatt
     4861 ttaatcgcgg atgatattca ggatagatca gaatctcgcc gtggtggcaa aacagcacat
     4921 attcaaatgc gtgattttca tgttaaaaag cacttaaaag gtgattcttt gcactttggt
     4981 gaagcaatgg ctataaacag ttttttgatt gctcagcatt acgctgctaa ccttatagct
     5041 acgttgccaa cctcagcaga tattcgtatt aaggccctac agaatgttaa taaatgtttt
     5101 attgttaccg ctcatggtca aacaatggac atttttgtag agtctaatgg agctgcatct
     5161 tcagaagaag taaaccatat tttagaatgg aaaacggcgt actatacatt tataaatcct
     5221 ttgcagcttg ggggcatttt ggccggagct cgtaaagcag acatggcaaa gctggcagac
     5281 tatggtttgc atgcggggcg ggcgtttcag cttgttgatg atattattgg tacttttggc
     5341 tcatctgata tgacaggtaa aagtccgcta gacgacataa aagaaggtaa acgtacatta
     5401 atggtactcg cagccctaga aaaagctaat gcttcagaca gttattttct agaagcatgt
     5461 cttggcagcc aacaacttac catgcacgat tttgaaaaat gccaaagcat tattacaaaa
     5521 acaggtgcgc tagattacac caaagaacaa gcagaacagt ctagcaaaaa agcaatagcc
     5581 gctattagta gcgaaacaac gtgggttcca caagcaaagc aatttcttat agatcttgct
     5641 caatacattc tcgtgcgtaa cacgtagcac aattgatgac attaaaaggt atactaaata
     5701 gagtatgaag aaaaaaatag caataattgg tgctggtttt ggcggcataa gcgctgccgc
     5761 atatttagcg cgtgatggct atgatgttac agtgttcgaa aaaaacagtc agccaggtgg
     5821 ccgggcaatt gtatataaac acaaaggctt tacgtttgat atggggccaa gttggtacat
     5881 gatgccagat gtatttgaag atttttttac agattttaat acttcgtcgg aaaaatacta
     5941 ccaactaaaa aaactccttc cgtcgtatcg ggtgtataac aatacaacgt cgcacgatgt
     6001 tagtccggta gatgaaggtg gcttggcggt atttgacgat cttgaaccag gtgcagccaa
     6061 gcaagtaaag caactgctta aaaaaacagg caaagaatac cgacacattc gtgaacacct
     6121 actagaaaaa gactatatct caccaataga cgcaattgat agtaaagcac tgtctatgct
     6181 tgcgaatccg gaattgtttt tttcgtacca tagccgtatt aaaaaaacgt cacgcaacca
     6241 agatattcaa aaaattctcg aattcatgac agtgtttatg ggtggcagcc cacagaatat
     6301 tccgggcgtc tatagtttgt taagtcacgt cgatttgagc cttggtatat ggtacccaat
     6361 gggtggtttt ggggctgtgg taaaaggttt tgagtcgatt gctaaacaac agggcgcaac
     6421 atttgtgtat aacgcagaag ttaccagaat tcatacgaca cagcatgctg tgtcgcacgt
     6481 tgaagttgct ggtgttaagc accagtttga tgctgttata gcaaatgccg attatcatca
     6541 cgtagaaaca aagctggtgc caagaaacta ccaatcgtat ccagaaaagt actgggccaa
     6601 aaaaacacta tcaccgtctg ctatgttggt gtacttgggg gtagacaaag tagtgcctgg
     6661 tttgttgcat catacaatgt tttttgatgc agactggcat ggtcattttg accaagtatt
     6721 ttcaaaaaaa gaatggtcgc tccgcccgct tttttatgtt tctaacccaa gtaagacaga
     6781 tccaacggta gcaccaaaag gcaaagaaaa tattatcatg cttgcaccgc ttgcgaatgg
     6841 cttgcaccca acaaaagatc aaataaatga actagtagaa agtttaatcg ctcgcctaga
     6901 agctcgtaca aaaacaaaca ttagcaaaca tattgtggtt aaaaaagttg ttctgatgga
     6961 cttttttgca gaaacattta acgcgtataa gggcaatgcc tttggtttag cgcatacact
     7021 aggccaaagt gcgatgttta ggccccgtat gcaaagtaaa aaattaaaga atttatatta
     7081 tgtagggcaa tacacaaacc caggtacggg cgtgccaatg gtagtgctat ctggtaaaat
     7141 tattgcaaga gtggtgggcg aaagggttcg ccagtgacaa gagaaaaaga gatatttaaa
     7201 caaggtagca caacatatta ctggagtagc gtattctttc cagagcagac aaaagaagac
     7261 gtatttaaac tttatagttt tgtacgtatt gccgataact ttgtagatgc caagccacag
     7321 aataaaaaat cttttattca attaaaaaaa gcgtggaccg cattggtgaa tataaataag
     7381 caaccgtcta aaaaaatgaa ttcagatatt cgcttggctg taactaatat gtatgacgta
     7441 tacaaaaagt atgatttttc taaagagtgg gtgagtgcat ttttagacgc catggaacaa
     7501 gatttaggaa aaaaaagata caaaacatta gacgacatca ttgcttacat gtacggctct
     7561 gcagaagtta ttgggctcat gatggcaaaa ataatcggtg taccagagcg gggctacgaa
     7621 gcggctcgta tgcaaggcag ggctatgcaa tatatcaatt ttatacgcga tatcgcagag
     7681 gataatcagc tgggtagggc atattttcca gcgaacgaac tcaagaaata cgggcttccg
     7741 agtgtttcta aagaagatgt gtatcggcac ccagcagcgt ttcgtgaatt tatagaagcc
     7801 caacttgcgt actatacaaa atggcaaaaa caggcagata aaggcatgaa atacataccg
     7861 cgatcgcagc gaatcgcact gcgtacagca gttgatatgt acaactggac agcagtagct
     7921 attgcaaaaa atccttttat agtgtttgat aaaaagataa aaccaagtaa aagccgtgtg
     7981 gtggtacggg cgttagtgag gtctatccat gcgtgaattt acatattact tatttaatct
     8041 actggttttt ttgccagttt tggtactgag ctttacgaca gatgtaaagc cacaccgtca
     8101 cgtacgtggc ttgttagctg gatttttatt agtgagcgta ccgtttatgc tgtgggacat
     8161 atgggcagcg caagcgggtc actggggctt taacgtggcc tacattacag ggccgtacct
     8221 gtttggtgta ccactagaag aatatttatt ctttattact gtgccattcg cgatgattta
     8281 tgtttgggga gtggtcaaaa aatatgttac cgaccgtgct acggctggct tctttccact
     8341 cttggccttt ggtatcacag cagggtattc ggtatggtta ctcgtgaact attggggtaa
     8401 tggctataca cgctcggttg caattgcagc tattattgct gttttagtgg cagcggtttc
     8461 acgtattgcc tatacgtggc gattttggac gtttcaggtt gtgctccttg ggctcttttt
     8521 agtatttaat tcacttctta caatgttgcc tattattact tatggcccag atgctattat
     8581 tggctttaaa attggcgata taccaataga agattttttg tttaactttg cctttattaa
     8641 tctgtttttg ctagtttaca catttgtaga tcagcccaga atagtcaaat aactacaaaa
     8701 gaccaaacat tgacaaaata gcaaaaatct gctatatttt atatatgaaa ccgagccccg
     8761 aagctcacca atatcaaaat gttacagata ctataagtta ttttggcaac acatatgcat
     8821 catggcctgt gtatacacaa gtagatcaat tgttaatggc gagtattggt gtaggcggta
     8881 gcatagcacc cgaagaacta ctaggggtaa tcaactctaa agaatgtagc ttgtcggcta
     8941 agattttcgc tgtg
//
LOCUS       DAOPLS010000007         8462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_8905_length_8462_cov_4.163435, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000007 DAOPLS010000000
VERSION     DAOPLS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8905_length_8462_cov_4.163435"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..397
                     /locus_tag="PKV52_00355"
     CDS             125..397
                     /locus_tag="PKV52_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98697.1"
                     /translation="MAKTKNKQKTPIEPDSVYFLKLVLYFLLGCLWIQVGGSNGISIP
                     VGLVIGIVFASHDNFQIDRKIEIAVLLIATVLSFIAPIGFVLTVCS"
     gene            497..2086
                     /locus_tag="PKV52_00360"
     CDS             497..2086
                     /locus_tag="PKV52_00360"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPD98698.1"
                     /translation="MATRYIFVTGGVLSGVGKGVTAASIGAVLKSRGLRVSIQKCDPY
                     FNVDAGTLNPAEHGEVFVTKDGAETDLDLGHYERFLNIELTQRSSTMSGRIYRQVIED
                     ERAGKYLGKTVQIIPHVTNAMQEAIIAAGKGYDVHIVEVGGTVGDYESTAFIEAIRQM
                     RTRIGPENCMYVHVVYLPFIAASQEVKSKPAQNAVRDLREAGIHCDLLVARADHDVPQ
                     SALKKLSLFSDIAIDKVVPMPTVDTIYEVPLILEKRGVGDIVCQHLGLPKATTNLDDW
                     AKLVNHIKDKKAPKVSVAIVAKYLDHTDTYTSVVEALKSAAWNSSHQVSITWIDAEKI
                     TKNNIAKLLDGMNGVVVPGGFGSRGIEGKIIAAQYALQNKLPYLGLCLGMQVACIGAV
                     RLSGTPTANSTEFDSDTEHPVIDLLESQKTLSGMGGTMRLGNYTCNLTKDSTVYKLYN
                     KSSILERHRHRYEFNPLYQASLEQQGVHIVGKNPDTGLTEVIEASNHPYYVATQYHPE
                     FNSRPLKPHPLFLGFIRACLA"
     gene            2332..2943
                     /gene="rplC"
                     /locus_tag="PKV52_00365"
     CDS             2332..2943
                     /gene="rplC"
                     /locus_tag="PKV52_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPD98699.1"
                     /translation="MKALITRKVGMTSIIADNGALTPVTLLSASPCVVTQVKTAETDG
                     YTAVQLGFEEAKKIGKAQAGHFKQSGITPKVVREFRIDEISDDITVGSKISADAFTVG
                     DTVHATGTSKGKGFAGTIKRHNFKRGRKTHGGQSYRRPGSIGSMYPQKIFKGKRMAGQ
                     MGHEQVTVRNLHVALVDTEHNVIGIVGAVPGPRKGIVILKGAN"
     gene            2943..3581
                     /gene="rplD"
                     /locus_tag="PKV52_00370"
     CDS             2943..3581
                     /gene="rplD"
                     /locus_tag="PKV52_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPD98700.1"
                     /translation="MAVSSYSASGTKSTTAVKLNKDIFAIEVANHELLKQAYITYKAN
                     GRLNLAKTLKKGEVSGGGKKPWAQKGTGNARTGSIRNPIWRGGGITFGPSGKENYTMK
                     LNASARKLSLRQALSLAVKNKPVCVIEDFVVKSGKTKDAATLLDKLGASAQALVVIES
                     ATPKTIQACANINTISLVKASNLSVVDVLDARNIVMTKAALAVIDQRLGGTK"
     gene            3581..3889
                     /gene="rplW"
                     /locus_tag="PKV52_00375"
     CDS             3581..3889
                     /gene="rplW"
                     /locus_tag="PKV52_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPD98701.1"
                     /translation="MNLVITPRVSEKAYGQASALNTYVFVVPLGANKIAVKRAIETEY
                     NVDVIDVNIARISGKTKKSYRKGGRTIVGTRSDTKKAYVRIKDGQTIPVFAAQEEEKN
                     "
     gene            3889..4728
                     /gene="rplB"
                     /locus_tag="PKV52_00380"
     CDS             3889..4728
                     /gene="rplB"
                     /locus_tag="PKV52_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPD98702.1"
                     /translation="MAIKVLKPNTPGQRGMTTQDFDEITTKKPVKSLLTIQKGSVGRN
                     NQGRITTRHRGGGVRRYYRHINFNLPVGTKATVEEIEYDPNRSARIARLKDHKGSYHY
                     IIAAQGLKKGDTVVSDEDAAVATANRLPLKSIPTGSTIYAIELQVGRGAQLVRSAGNG
                     AQLLAKEGEYAQVKLPSGEVRKIHINCSAHIGNVGNVQHQNVKIGKAGRNRRKGIRPS
                     VRGVVMNAVDHPHGGGDGGRHRMGKAPRTPWGQKTLGFKTRSRKASNSFIVKSRHQAK
                     RRG"
     gene            4732..5001
                     /gene="rpsS"
                     /locus_tag="PKV52_00385"
     CDS             4732..5001
                     /gene="rpsS"
                     /locus_tag="PKV52_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPD98703.1"
                     /translation="MSRSLKKGPFVDPKLAKKVAALGSEDRTIIKTWARSSTVSPDFV
                     GKTIAVHNGRVHVPVFITENMVGHKLGEFAPTRKFRSHGGKLAKK"
     gene            5012..5398
                     /gene="rplV"
                     /locus_tag="PKV52_00390"
     CDS             5012..5398
                     /gene="rplV"
                     /locus_tag="PKV52_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPD98704.1"
                     /translation="MNVQAVAKGVRMSPRKVGVVAALVRGRTVADAMVILDHTPRRSA
                     DPVYKAVASAKANAEHNHNLKPDTLKIVEISVSPGPRLKRYRPAARGRALPFQRRTSH
                     IRVVVTGDERVAKKTAAKPATKKEAK"
     gene            5398..6027
                     /gene="rpsC"
                     /locus_tag="PKV52_00395"
     CDS             5398..6027
                     /gene="rpsC"
                     /locus_tag="PKV52_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPD98705.1"
                     /translation="MGQKVNPISMRLQVNKNWRSKWFAPKREYAHYLAQDLAVRRYVA
                     KKFTNRSAINTVDIERSPNLVTVTIQTAKAGVVIGRGGAGAQELKAEFERIFGTTVRV
                     NIEELKKPELYAKLVAENIANQLERRIAFRRAMKSSAAATMRAGAKGVRIEVAGRLNG
                     AEMSRREKLINGSVPLHTLRADIDYGYERAHTPAGIIGVKVWIYKGEAK"
     gene            6027..6443
                     /gene="rplP"
                     /locus_tag="PKV52_00400"
     CDS             6027..6443
                     /gene="rplP"
                     /locus_tag="PKV52_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPD98706.1"
                     /translation="MLLPKKMKHRKEFKGKIRGHATRGNYIAHGDYAIQSLGHDRITS
                     RQIESARQAMTRYIKRGGKIWIRIFPQTPVTRKPQDVKMGSGKGSPEFFAAKVKPGTV
                     LFEMQGVTEETAREAMRLAAHKLPVKTKFIIRGEES"
     gene            6448..6708
                     /gene="rpmC"
                     /locus_tag="PKV52_00405"
     CDS             6448..6708
                     /gene="rpmC"
                     /locus_tag="PKV52_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPD98707.1"
                     /translation="MADKKATKKVPSKAAKKDTKDTLKKVDFKKMSEAELSKAIEALR
                     EDLVTLKRGTVMGEVQNVHAYGDRRKELARALTARNTAREVK"
     gene            6709..6987
                     /gene="rpsQ"
                     /locus_tag="PKV52_00410"
     CDS             6709..6987
                     /gene="rpsQ"
                     /locus_tag="PKV52_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPD98708.1"
                     /translation="MAHTITGRVSSDKTDKTIVVSVVTRKTHPIYKKQYSVTTKFMAH
                     DEDNQAKEGDLVSITEHRPISKRKRWTLVSVLETAQTKHIEPEVQEEA"
     gene            6990..7358
                     /gene="rplN"
                     /locus_tag="PKV52_00415"
     CDS             6990..7358
                     /gene="rplN"
                     /locus_tag="PKV52_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPD98709.1"
                     /translation="MIQQESRLGVCDNSGAKEILCIRVLGGTRRRYARVGDVIVATVK
                     QAAPNGNVKKKSVVRAVIVRTTNVIRRADGSTIRFDDNAAVIIDDDKQPKATRIFGPV
                     PRELREQGYAKIISLAPEVL"
     gene            7358..7687
                     /gene="rplX"
                     /locus_tag="PKV52_00420"
     CDS             7358..7687
                     /gene="rplX"
                     /locus_tag="PKV52_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPD98710.1"
                     /translation="MTQLYKIRLKKGDTVVVRSGKYKGQTGKVLATHPAENKVTVEGI
                     NIVKKHVKPNNKYPQGGIIELTKAIDVSKVGIQDPTTKKASRIAYSFDKSGKKTRIYA
                     SSKKEVK"
     gene            7687..8265
                     /gene="rplE"
                     /locus_tag="PKV52_00425"
     CDS             7687..8265
                     /gene="rplE"
                     /locus_tag="PKV52_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPD98711.1"
                     /translation="MVKTKQPTNARLKVQYNTTYRAELRTELGLSNINQVPKLEKIVV
                     NVGLGRAKDDKKLMEVATNTLIRITGQKPIQTTAKKSIASFKLREGNKIGLKVTLRGE
                     MMYEFLDRVVNIVLPRLRDFHGVSAGAFDKTGNYSLGIKDQSVFPELNFEETTTIHGL
                     QINFVFSNVTSVNQSKALLAKFGMPFEKERKA"
     gene            8268..>8462
                     /locus_tag="PKV52_00430"
     CDS             8268..>8462
                     /locus_tag="PKV52_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HPD98712.1"
                     /translation="MAKKSIVLRDQKRQAMHAKYAAKRAELKKLGDYEGLHRLPRNSS
                     PTRLKNRCSETGRPRAYMRQF"
BASE COUNT         2765 a         1714 c         1905 g         2078 t
ORIGIN      
        1 gatatttgga acaggcctcg tagtacgtat cgcaaccgaa cgctacagta ttgctgctag
       61 tagctacaaa aaacgcctgc aaaaaaccta atacgccacc actctatgat ataattacta
      121 ggcaatggcc aaaactaaaa ataagcaaaa aacacctatt gaaccagata gcgtatattt
      181 tttaaaactg gttctatact ttttacttgg ctgcctttgg atacaagtcg gtggcagtaa
      241 cgggatttct attcctgttg gcttagtcat aggcattgta tttgccagcc atgataattt
      301 tcagatagat agaaaaatag aaattgctgt cttactcata gcgacagtgc ttagctttat
      361 tgcaccaata ggcttcgtac ttacagtgtg cagctaaaaa atacttgctt tttttgctgg
      421 tggtatcata attaaagaaa acccctgaaa aaacatatat gtgtgaataa aaaagtttga
      481 tacgaggagt gttcttatgg caacgagata tatttttgtt actggtggtg tgctaagtgg
      541 cgtaggcaaa ggagttacag ccgcaagtat cggagcagta ttaaaaagtc gtggtttacg
      601 tgtgagtatc caaaaatgcg acccctattt taatgtagac gccggtacac tcaaccccgc
      661 agaacatggt gaagtatttg ttacgaaaga tggtgcagaa actgatctcg acctaggcca
      721 ctatgagcgt tttctaaata ttgagcttac tcaaagaagc tcaacgatga gtggtcggat
      781 ttaccgccag gtaatcgagg acgaacgagc aggcaagtac ttaggcaaga ctgtacagat
      841 tattccccac gttacaaatg ccatgcaaga agcgataatt gctgccggca aaggctacga
      901 cgttcatatt gttgaagtag gtggtactgt tggcgattac gaaagtacag ctttcattga
      961 agcgatacgt caaatgcgca cacgtattgg acctgaaaat tgcatgtatg tacacgtagt
     1021 atatttacca tttatagccg cgagtcaaga agtaaaaagt aagccagctc aaaatgcggt
     1081 ccgtgactta agagaagctg gtatacattg tgatttactc gtagcccgcg cagaccatga
     1141 tgttccacaa agtgccctaa aaaagttaag tttatttagt gatatcgcga ttgataaagt
     1201 agtccctatg ccaacagtag ataccatata tgaagtaccc cttatattag aaaaacgagg
     1261 tgtcggtgat attgtctgtc aacatttagg tttgccaaaa gcgacaacta atcttgacga
     1321 ttgggcgaag ctggttaatc atataaaaga taagaaagcg ccaaaagtgt cggtggcaat
     1381 agttgccaaa tatcttgacc atacagatac atatacaagt gttgtagagg cgttaaaaag
     1441 tgctgcctgg aacagtagcc accaagtatc tattacatgg atagatgcag aaaaaataac
     1501 aaaaaacaac attgccaaat tacttgacgg catgaatggc gtggttgtac ctggtggttt
     1561 tgggtcacgg ggtatcgaag gaaaaattat agctgctcaa tatgcccttc aaaacaaact
     1621 gccatattta gggctatgtc taggaatgca agttgcctgt ataggagcag tacgtcttag
     1681 tggcacgccc acagcaaatt caaccgagtt tgacagcgat acagagcatc cagtaataga
     1741 tttactagaa tcgcaaaaaa cactcagcgg tatgggcggg actatgaggt taggcaatta
     1801 cacatgtaac cttaccaaag acagtactgt ctacaagcta tacaataagt cttcaatcct
     1861 agagcgacac cgtcaccgct atgagtttaa ccctctctat caagcaagtt tagaacaaca
     1921 gggtgtacat attgtgggta aaaaccccga tactggtcta acagaagtta tagaagcatc
     1981 taatcatcca tattacgtcg ctacacaata ccacccagag ttcaatagtc gtccacttaa
     2041 accccatccg ttatttttgg gttttattcg tgcctgtttg gcataagtag ccatgcacat
     2101 gctcatgcac gtcaaagtag ccattcggta atgtatattt gttttataaa taaccctttg
     2161 acttatctgt aataatttga tagaatttga tggtatataa aaacagctcc ttggtgaaat
     2221 ggtaagttga gcgcactatt tgcctcaaaa ctaccaatac taccaagcat ttttatgccc
     2281 tatacacggt agtgtgctat tgaggtgagc gtaaataatt taaggagtac tatgaaagca
     2341 ctcattacaa ggaaagttgg catgacgagt atcattgctg acaatggtgc attaacacca
     2401 gttaccttgc tttccgctag tccctgtgtg gtgacacagg tcaaaaccgc agaaaccgac
     2461 ggttatactg cagtgcagct cggctttgaa gaagccaaga agatcggcaa agcccaagcc
     2521 ggtcatttta agcaatcggg catcacccct aaggtagtgc gcgaatttcg tatagacgaa
     2581 ataagtgatg atattaccgt aggatcaaaa atcagtgcag atgcatttac tgttggtgac
     2641 acagttcatg caactggtac cagtaaaggc aaaggttttg ctggcactat taaacgacat
     2701 aattttaaac gtgggcgtaa aacccacggt ggacaaagtt atcgtcgccc aggatctata
     2761 ggatctatgt atcctcagaa aatttttaaa gggaaacgaa tggctggcca aatgggccac
     2821 gaacaagtta cagttcgtaa cttacatgtt gctcttgtag ataccgaaca caatgttatt
     2881 ggcattgttg gtgcggtccc agggccacgt aagggtattg ttatcctgaa aggagcaaac
     2941 taatggccgt atcttcatat agtgcgagcg gcaccaagtc aacgactgct gtaaagctca
     3001 ataaggatat atttgcgata gaagtcgcta accacgaatt gcttaagcaa gcgtacatta
     3061 cgtataaggc gaatgggcgc ttgaatcttg ccaagacact taaaaagggc gaagtaagcg
     3121 gtggtggtaa gaagccatgg gctcaaaaag gaacaggcaa cgccagaacc ggttcaatac
     3181 gaaaccctat ttggcgtggc ggtggaatta cctttggccc tagtggcaaa gaaaactaca
     3241 ccatgaagct aaatgccagt gcacgcaaat tatctttgcg acaagcactg agcctcgcag
     3301 tgaaaaataa accggtgtgt gttattgaag actttgttgt caagagcgga aaaactaaag
     3361 acgcagccac gttattagat aaactcggtg catctgccca agcgctcgtt gttatagagt
     3421 ctgccacacc aaagacaatt caggcgtgcg ctaacatcaa taccatttca cttgtcaagg
     3481 caagtaatct atcagttgta gatgtcctag atgcacggaa tattgttatg actaaagctg
     3541 cacttgctgt gatagatcaa cgtctaggag gtaccaaata atgaatttag ttattactcc
     3601 acgtgtcagc gaaaaagcat acggacaagc tagtgcgctc aatacctatg tttttgttgt
     3661 acctcttggt gctaacaaaa tagcagtaaa acgtgcaatt gaaaccgaat ataacgttga
     3721 tgtgatagat gttaacatag cacgtatttc tggcaagact aagaaaagtt accgcaaagg
     3781 cggccgtact atagttggca cacgttcaga tacgaagaaa gcatatgtac gaataaaaga
     3841 cggtcaaacc attcctgtgt ttgccgccca ggaagaggag aaaaactaat ggccataaaa
     3901 gtactgaagc caaatactcc tggccaaaga ggcatgacta cccaggattt cgatgaaatt
     3961 acgacaaaaa agcctgtaaa gagtttactt acaatacaaa aagggtcggt tggacgcaat
     4021 aaccaaggtc gtattactac acggcatcgt ggtggaggag ttcgccgtta ctaccgtcat
     4081 ataaacttta atctccctgt tggcacaaag gccactgttg aagaaataga atacgaccca
     4141 aaccgtagtg cccggatagc tcgcttaaaa gaccacaaag gatcatacca ctacattatt
     4201 gctgctcaag gacttaaaaa gggtgacaca gttgtttctg atgaagatgc cgctgtagct
     4261 accgctaaca gattaccact gaagagtatt cctacaggta gcacgatcta tgcaatagaa
     4321 ttgcaagttg gccgtggagc acaacttgtt cgtagcgccg gtaatggtgc tcagctgctt
     4381 gcaaaagaag gtgaatacgc ccaagtaaaa cttccaagtg gtgaagtccg taaaatacat
     4441 attaattgca gtgcacatat cggcaatgtt ggtaatgtac agcatcaaaa tgtcaaaatc
     4501 ggtaaagctg ggcgcaatcg tcgcaagggc attcgcccta gtgttcgcgg tgttgtaatg
     4561 aatgctgtag atcaccctca cggtggtggt gatggtgggc gtcatcgtat gggcaaagcc
     4621 ccacgaacac catggggtca aaaaactctt ggttttaaaa cccgtagccg taaagcatct
     4681 aattcattta tagttaaaag tcgtcatcag gcgaagagga gaggttagtc aatgagtcgt
     4741 tcattaaaga aaggaccatt tgtagatcct aagcttgcta agaaagtggc ggctttaggc
     4801 tcagaagatc gcaccattat taaaacgtgg gctagatctt cgacagtttc accagacttt
     4861 gtcggtaaaa caattgcagt acacaacggt cgagtgcatg tacctgtatt cataacagaa
     4921 aatatggttg gacataagct aggcgaattt gccccaactc gtaagttccg cagccatggc
     4981 ggtaaattgg caaagaagta ggagtatcac tatgaatgta caagcagtcg caaaaggtgt
     5041 tcgtatgagt ccccgcaaag tcggagttgt agccgcttta gtacgcggtc gcacagttgc
     5101 agatgcgatg gttattttgg accatactcc tcgccgttca gccgatccgg tatataaagc
     5161 cgtagcaagt gctaaagcta acgcagaaca taatcataat cttaaacccg atactcttaa
     5221 aatagtagag attagcgtat cgccaggacc acgcctaaaa cgatatcgac ctgcagcaag
     5281 gggacgggct ttaccattcc agcgtcgaac gagtcatatt cgtgttgtcg taactggtga
     5341 tgagcgagtt gcaaaaaaga cagctgccaa gccagctacc aaaaaggagg ctaagtaatg
     5401 ggccaaaaag ttaatccaat tagtatgcgc cttcaggtca ataaaaactg gcgtagtaaa
     5461 tggttcgcac caaaacgcga atatgctcat tatcttgcac aagatcttgc agtgcgcaga
     5521 tatgtagcaa aaaagtttac taatcgtagc gctattaaca cggtagatat agagcgctca
     5581 cctaatctcg ttactgttac tattcaaaca gcaaaagctg gtgttgttat tggccgcgga
     5641 ggtgcaggtg cacaagaact caaggccgaa tttgaacgca tatttggcac gacagtgcga
     5701 gtaaatatag aagaacttaa aaaacccgaa ctctacgcta aattagtcgc agaaaacatt
     5761 gcgaatcagc tcgagcgacg cattgctttt cggcgagcta tgaaaagctc tgcagctgcc
     5821 actatgcgag caggggcgaa aggcgtgcgt attgaagtag ctggtcgtct taatggtgca
     5881 gaaatgtcac gccgtgaaaa acttattaac ggcagtgtgc cacttcacac actacgcgca
     5941 gacatcgact atggatatga acgagcacat acaccggctg gaattattgg cgttaaggtg
     6001 tggatatata aaggggaggc caagtaatgt tacttcctaa gaaaatgaag caccgcaaag
     6061 aatttaaggg caaaatacgc ggtcatgcga caagaggtaa ttatattgcc cacggtgact
     6121 atgcgattca atctttaggt catgatcgaa ttaccagccg acaaattgaa tctgctcgtc
     6181 aagcgatgac ccgttatatc aaacgtggtg gaaaaatttg gatacgtata ttcccacaaa
     6241 cacccgttac acgtaaaccg caagacgtaa aaatgggtag cggtaaaggt tcaccagaat
     6301 tttttgcagc taaagttaag cccggtacag ttttatttga aatgcaaggc gtaacagaag
     6361 agacagcacg tgaagctatg cgtttagcgg ctcacaaact tcctgtcaag accaaattta
     6421 taatacgtgg agaggagtct taatcgtatg gcagacaaga aagctacaaa aaaagtacca
     6481 tcaaaagcag ccaagaaaga cacgaaagat actctcaaaa aggtagattt taaaaaaatg
     6541 agtgaagcag agctgtcaaa agcgattgaa gcgcttcgtg aagatcttgt aacacttaaa
     6601 cgtggcactg ttatgggtga agtacaaaat gtacacgctt atggcgaccg ccgcaaagag
     6661 ctggcacgtg cattgactgc gcgtaacacc gcaagggagg ttaagtaaat ggctcacaca
     6721 attacaggtc gtgttagttc cgacaaaacc gacaaaacaa ttgtcgtgag tgtcgtaaca
     6781 cgcaagacac atccaatcta taaaaagcag tattctgtga cgactaagtt tatggcacac
     6841 gatgaagata accaagcaaa agaaggcgac cttgtttcta tcactgagca tcgtcctatt
     6901 tctaagcgta agcgatggac tctggtgagt gttctagaaa ctgcacaaac aaagcatatc
     6961 gaaccagaag ttcaggagga agcgtagtta tgattcagca agaatcacgc cttggtgtct
     7021 gcgacaatag tggtgcaaaa gaaattttgt gcatccgtgt tctgggtggt accagacgac
     7081 gctacgcccg agttggcgac gtaatagtgg caacagtcaa acaagcagca ccaaacggaa
     7141 atgtaaagaa aaaaagtgtc gtccgggctg ttattgtacg tacaaccaat gtcatacgac
     7201 gcgccgatgg ttcaaccatc cgctttgatg ataatgcagc tgttattatt gatgacgata
     7261 agcaacccaa agcaacacgc atattcgggc cagttccacg cgaattacgt gaacaaggct
     7321 atgcgaaaat tatcagtctt gcaccggagg tcctctaatg acacaactct ataaaatcag
     7381 attaaaaaaa ggcgacacag tcgttgtgcg ctcaggcaaa tacaaagggc aaactggtaa
     7441 ggtacttgca actcacccag cagagaataa agttactgta gagggcatca atattgtaaa
     7501 gaaacatgtt aaaccgaaca ataaataccc tcaaggcggt atcatagagc tcacaaaggc
     7561 aatagacgta tccaaggttg gtattcaaga tccaacaacc aaaaaagctt cacgaattgc
     7621 ctatagcttt gataaatcag gcaagaaaac ccgcatatat gcctcatcca agaaggaggt
     7681 gaagtaatgg ttaagacaaa acaacctacc aacgcacgtc taaaggtcca atataatacg
     7741 acataccgtg cagaactacg tacagaactt ggtttatcta atataaatca ggttcctaag
     7801 ctagaaaaaa ttgttgtaaa tgtcggcttg ggtcgcgcta aagatgataa aaagctgatg
     7861 gaagtagcaa ccaacacact tatccgcatt accggacaaa agcctattca aacaactgct
     7921 aaaaagagta ttgctagctt taagctacgt gaaggtaaca aaataggact aaaagtcacg
     7981 ttacgcggcg aaatgatgta tgagtttctt gatcgtgttg tcaatatagt gttaccacgg
     8041 ttacgggact ttcatggtgt ttctgcagga gcatttgata aaacaggaaa ttacagcctc
     8101 ggtataaaag atcaatcggt ctttccagaa ttaaatttcg aagaaacaac gaccatacat
     8161 ggacttcaaa tcaattttgt ctttagtaat gtgacatctg taaatcaaag taaggcattg
     8221 ctcgctaaat tcggcatgcc gttcgagaaa gaaaggaaag cgtaaatatg gcaaagaaat
     8281 caattgtttt acgtgatcaa aaacgacagg caatgcatgc taaatacgct gcaaaacgag
     8341 ctgagcttaa gaaactaggt gattacgaag gactccatag attaccacga aacagtagtc
     8401 caacacgcct taagaatcgc tgtagcgaga ccggtcgtcc tcgtgcctat atgcgccaat
     8461 tt
//
LOCUS       DAOPLS010000008         8337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_9085_length_8337_cov_4.095508, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000008 DAOPLS010000000
VERSION     DAOPLS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9085_length_8337_cov_4.095508"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKV52_00435"
     CDS             complement(<1..319)
                     /locus_tag="PKV52_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPD98713.1"
                     /translation="MYKYLKFFEELSIDDIPQVGGKNASLGEMYRHLRPQGVSLPNGV
                     ATTADAYRYFLESAGLNQGITDTLQGLDVSNVHDLAHRGTIIREMIINAQLPQDFQDE
                     IKQG"
     gene            422..1033
                     /locus_tag="PKV52_00440"
     CDS             422..1033
                     /locus_tag="PKV52_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPD98714.1"
                     /translation="MDTHIQTIKTWLGTGSINIFGLPFAGKDTQGRLFANYLEGVLIS
                     GGDILRKSQSNLELQRIMAAGEIIPTSLWEEIVVPFLANPEFAQKPLILSEVGRLQGE
                     ERIVEKATLATGHPQKAVILLQLTDQEVWNRFDAAQQEGDRGERADDRRDVIQTRLDS
                     YHHKVMPVIEHYRSNGLLIEVDGAQSRQDVTEQIITSLVARAT"
     gene            complement(1026..2705)
                     /locus_tag="PKV52_00445"
     CDS             complement(1026..2705)
                     /locus_tag="PKV52_00445"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPD98715.1"
                     /translation="MARIYFYDSTEIDKQQLTNGLIGTDHHWEFVDEPISLENLDPET
                     EVISVFITSSVTAEIIQALPKLRLIACRSTGYNNINMNAAEERNIIVENVPTYGEETV
                     AEYAFTLLLALSRKLVATLKSFQDNTPLDKLCGWDLHGRTLGVIGTGHIGRSSIKIAN
                     GFGMNVVAYDPYPNKDAAKELGFQYLTLDEVMSQSDAITIHAPYFPSNKHLIGSDQLA
                     RMKPTAVLINTARGELIDTKALLEVLSKKKIAGAALDVVEGERLIHVDDDVELLYRSN
                     VVGSDMLKYGVEILALSHMENVILTPHNAFNTLEAVGRINAVTCENIIKFWYGDTPNV
                     VKPPAKQTGKLTIVRHTESEWNATGQWTGITDVHLSGKGFRDAGYLGQEVAIQNLTFD
                     KAYCSQQIRTLETIEGILNASGQLDLEYEREAALNERDYGDYTGKNKWEVRDLIGEEK
                     FQRIRRGWDEPVPNGETLKDVYKRVVAFYKKTILPQLLDGKNILIVSHGNGIRALIKY
                     IESISDEDIEKVEMIFNTILVYDVDKNGHMTHKTTHVVEEKVPANSSPTTT"
     gene            2755..4026
                     /gene="eno"
                     /locus_tag="PKV52_00450"
     CDS             2755..4026
                     /gene="eno"
                     /locus_tag="PKV52_00450"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPD98716.1"
                     /translation="MEIINIIARQILDSRGVPTVEADVLLANGSSGRAAVPSGASTGI
                     HEAHELRDGEAAYHGQSVYKAVDNIHTHILPALRGIPADDQFKIDQIMIDLDGTPNKA
                     QLGANAILAVSLAVAHAAAKSRGILLYKHIADIAQPQQLLLPMPMLNVLNGGKHANGS
                     TDIQESMIMPLSAHTFSQALQMSVEIFQELKMLIHSKGYATTVGDEGGFAPHLKNGNA
                     EAFDLLSEATDKAGYTPGQDVAFAVDVAASELYKDGSYHFTSERKHFNTQDLIEWFKR
                     VVSQYPIVSIEDGLAEDDWDGWKHLKVALPTTQLVGDDLLVTNTERLQYAIQEDAANA
                     ILIKPNQIGTLTETIRAIQMAKENGWNTIVSHRSGETEDVSIVHIAIGTGAGQIKTGS
                     LSRSERVAKYNELLRLEAIDSSLLLQNPFTS"
     gene            complement(4147..4461)
                     /locus_tag="PKV52_00455"
     CDS             complement(4147..4461)
                     /locus_tag="PKV52_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98717.1"
                     /translation="MTIERRVYSEQDIVQELLEESRERGQELTHEQLNIAYESLQRVM
                     KRNNERGTSKRYPDTMGVAAYAQRVSPEAYDSFTQAEQAAIVAESIHRFRNAKVLIPD
                     LQ"
     gene            4990..5064
                     /locus_tag="PKV52_00460"
     tRNA            4990..5064
                     /locus_tag="PKV52_00460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5023..5025,aa:Glu,seq:ttc)
     gene            5372..6256
                     /locus_tag="PKV52_00465"
     CDS             5372..6256
                     /locus_tag="PKV52_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD98718.1"
                     /translation="MTNLTKLYGQYRGVKNINLTLQHGEVFGFLGPNGAGKSTTIRTI
                     LNFITPSEGSATIYGLDSVKDSVEIKNNIGYLAGDIALYDNMTGRNLLKFLTRLGKQT
                     DWAYVDELAHRLDANLVRPIHTLSKGNVQKIGLIQAFMHKPSLLILDEPTSGLDPLMK
                     QVFYDMVAELKAQGKTIFVSSHDLTEVQKICDRAAFIRQGKLIAIEDIQTAKAINLRK
                     YRARFAKKVDAKPFVHLANVSDVEVAGDELTLTVTGSIEAFITELAKHKPQDLNEQEV
                     ALEDLFIHYYKDESDNNA"
     gene            6234..7040
                     /locus_tag="PKV52_00470"
     CDS             6234..7040
                     /locus_tag="PKV52_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPD98719.1"
                     /translation="MSQTIMRNTIYSKAVWDRKISTGVWSISMAAFALMFAWVFEIYA
                     GEITNFSDNFPAELSAVIGDLAAATTPAGFLAVELYSLFLPLIVAIVGITFGAAAIGK
                     EEESGTLELLLASPISRRKILVQKFGAIATVLFVIPFATWLGIVVGKSIFPFDVNVWH
                     VALASFASFFLGMVYASAAFAGQSVSGKRGIGLGIGGGLLAVTYIADVVSKLVDRLEF
                     LQYISPFYYFDISNVLFGNGRIGNFAVLAGLVAVFYTVVHIAFKNRDTGV"
     gene            complement(7400..>8337)
                     /locus_tag="PKV52_00475"
     CDS             complement(7400..>8337)
                     /locus_tag="PKV52_00475"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPD98720.1"
                     /translation="QAAKVALDKNGKFMFAVMTPCGNPVTGKAVEPPKPKVQAISCDA
                     LKVTLDTTKKSVSASVSGSASNTKIDGYKIDFGDGTVVNKQSANHTYAADGKYTIVAH
                     VSGKVNDKTKTVTSAGCTKSVEFVIPPTPEPKPEPKPEHQAIKCTTLTLDAVNKEKKY
                     VKVTVKGTAENTKIDSYSINFGDGSKAVNKQTAEHTYTTYGDFKIVASVTGKVGGKDV
                     TVSGVNCTQTVSFAEVPVACPTNPSLPIDDPNCKPCPTNPELNFDDPKCTETPVTPTP
                     ELPNTGAGSVIGIMSGVSILGAFGHRLWISRKLGL"
BASE COUNT         2331 a         1710 c         1734 g         2562 t
ORIGIN      
        1 aaccttgttt aatttcatct tgaaaatcct gaggtaattg ggcattaata atcatttcac
       61 ggataatagt accccgatgt gctaaatcat gaacattact aacgtctagg ccttgtaagg
      121 tatctgtaat accctggttt aagccagcgc tttcaagaaa atatcggtaa gcatctgcag
      181 tcgttgctac cccattcggc aaactcactc cctgcggtct taagtgtcga tacatttcgc
      241 caagagaagc gtttttgcca cctacttgcg gtatgtcatc aattgatagt tcctcaaaaa
      301 attttaaata tttgtacatc tgttccacct tgttgttatt agtatccttt ttataatacc
      361 actaatgctt ataattttct tgagtgaaac acaagtttta ttgtggtgat atactatttc
      421 tatggataca cacatacaaa caatcaaaac atggctcggt acaggatcaa taaacatctt
      481 tggcttgcca tttgcgggta aagatactca gggtcgtctt tttgcaaact acttagaggg
      541 ggtgcttata tctggtggcg atatactccg taagagtcaa agtaacctag agctacaacg
      601 cattatggca gctggcgaga ttattcctac cagtttatgg gaagagattg ttgtgccatt
      661 tttagcaaac cctgaatttg cacaaaaacc gcttatttta agtgaagttg ggcggttaca
      721 gggcgaagaa cgcatcgtag aaaaggcaac cttagcgaca ggtcacccgc aaaaagccgt
      781 tattttactc cagctgaccg accaagaggt atggaacaga tttgatgctg cccaacaaga
      841 gggtgatcga ggtgagcgtg cagatgatcg tcgtgacgtt atacaaaccc gtttagatag
      901 ttatcaccat aaagtgatgc ccgttataga gcattaccgt tctaatggat tgctcataga
      961 agtagacggt gcacaatctc gccaagatgt cacagagcaa attattacaa gtttagtcgc
     1021 acgagctacg tagtcgtagg gctactattg gcgggtactt tttcttctac aacgtgggta
     1081 gttttgtgtg tcatgtggcc gtttttatcg acatcataaa caagaatggt gttaaatatc
     1141 atttcaactt tttctatgtc ttcatcacta atagattcaa tgtatttaat gagcgcccga
     1201 ataccgttac catgagatac aattaatata tttttgccat ctagtagctg cggtagaata
     1261 gtttttttat aaaatgcaac aactcgtttg tagacatcct tgagtgtttc gccatttggt
     1321 acaggttcat cccaaccacg cctaatgcgc tgaaactttt cttcaccaat taaatcacga
     1381 acttcccact tatttttgcc agtatagtca ccgtaatcac gctcgttaag tgcagcttct
     1441 ctttcatact ctaaatctaa ctgcccagaa gcgtttaata taccttctat tgtttctagc
     1501 gtgcgtatct gttgcgaaca atacgctttg tcaaatgtca ggttttgtat agctacctct
     1561 tggcctaagt agccagcatc acgaaagcct ttaccactta agtgtacgtc ggtaattcct
     1621 gtccactgac cagttgcatt ccattctgat tcagtatgtc gcacgatagt tagttttcct
     1681 gtttgtttcg caggtggctt aacaacattc ggcgtatcgc cataccaaaa tttgatgata
     1741 ttttcgcagg taacagcatt gattctaccg actgcttcta gtgtgttaaa cgcattgtgg
     1801 ggggtaagaa taacattttc catatggcta agtgctagta tttctacacc gtacttaagc
     1861 atgtcagagc caactacatt gctacgatag agcaactcaa cgtcgtcatc tacgtgtatt
     1921 aaacgttcac cttcaactac atccaaggct gcgccagcaa ttttcttttt agacaagacc
     1981 tcaaggagtg cttttgtatc tataagttca cctcttgcgg tatttattaa aaccgcagtt
     2041 ggtttcatac gggctaattg atccgaacca ataagatgtt tattagatgg aaaataaggt
     2101 gcatgaatcg ttatagcatc actttgtgac ataacttcgt ctagtgtaag atactgaaag
     2161 ccgagttctt tggctgcatc tttattggga tacggatcat acgcaaccac attcatacca
     2221 aacccgttcg ctatttttat tgacgatctg ccgatgtgcc ccgtcccaat aaccccaagt
     2281 gttcgtccgt gtaaatccca accgcatagt ttatctaacg gagtattgtc ttggaacgat
     2341 tttagcgtag cgacaagctt tctcgatagt gccagcaaca atgtaaacgc atattcagca
     2401 acggtttctt cgccgtaggt aggaacgttt tctactataa tatttctctc ttcggcagcg
     2461 ttcatgttga tattgttata gcctgtagaa cgacatgcaa ttaagcgcaa ctttggtagt
     2521 gcctgtatta tttctgccgt caccgaactg gttataaata cagatataac ttctgtttct
     2581 gggtctaggt tttctagtga tattggttca tcaacgaatt cccaatgatg atccgtgcct
     2641 ataagcccat tggttagttg ttgtttatct atttctgttg agtcataaaa atatattcgt
     2701 gccattacgt atactattat ggcataagca tgagctttat gagaacatat tggtatggaa
     2761 attataaata ttattgcccg ccaaatatta gattcacgtg gtgtccctac tgtagaagca
     2821 gacgttttac tcgcaaatgg ctcaagcggt agagcagccg tgccatctgg tgcatctacc
     2881 ggcatacacg aagcacatga actaagagat ggcgaagcgg cttatcatgg tcaatctgta
     2941 tataaagctg tagacaatat tcacacacat atattgccag ccttgcgcgg tattccggct
     3001 gacgatcagt tcaaaataga tcaaattatg attgatctag acggcacgcc aaataaggca
     3061 cagctcggcg caaacgccat attggctgta tctttagctg tagctcacgc tgcagcaaaa
     3121 tctcgtggaa tattgttgta taagcacatt gccgatattg cgcaaccaca gcagctattg
     3181 ttacctatgc caatgttaaa cgtgcttaac ggcggtaaac atgcaaatgg ttctacagat
     3241 atccaagagt caatgataat gcccctaagc gcccacacgt ttagccaggc gttgcaaatg
     3301 agtgtagaaa tatttcaaga gctcaaaatg cttattcaca gcaaaggcta tgcaactact
     3361 gttggtgacg aaggtggttt tgccccacat cttaaaaatg gtaacgccga agcatttgat
     3421 ttactatcag aagctacaga taaagctggg tacaccccag ggcaagacgt ggcctttgcg
     3481 gtagatgtgg cagcaagcga gctgtacaaa gatggttcat atcattttac gtcagaacgt
     3541 aaacatttta atactcaaga tctaattgaa tggtttaagc gtgtagtaag tcaataccct
     3601 attgtatcta tagaagacgg tttggcagaa gacgattggg acggctggaa gcaccttaaa
     3661 gtggcactac caaccacaca gctggttggt gatgatttgt tagtaacaaa caccgaacgc
     3721 ctgcaatacg ctatacaaga agacgctgcg aatgccattt taataaaacc aaaccaaata
     3781 ggtacgctta cagaaacaat tcgggcaatt caaatggcaa aagaaaacgg ctggaacaca
     3841 atagtgtcgc accgctcggg tgaaacagaa gacgtatcta ttgtacatat tgccattggc
     3901 actggtgccg ggcaaattaa aacagggagc ctttcgcgca gtgaacgtgt cgccaaatat
     3961 aacgaattac tccgcctaga ggccatagat agttcgttgc tattgcaaaa tccttttacc
     4021 tcttaaatca agtatttata tcactaaaaa tatcacgaat aaaaaatatt attgtgattg
     4081 ttaaaatttt ctaccaaaaa acttcaacga taaaagtatt tgctcaccag attatagaag
     4141 aaacttctat tgtagatcag gaattaacac tttagcgttg cggaatctat gtatagattc
     4201 tgcgacgata gctgcctgct ctgcttgtgt aaagctatca taagcttcgg gtgatactct
     4261 ttgagcataa gctgcgacac ccatagtgtc tgggtatctc tttgaagtac cacgctcgtt
     4321 attacgtttc atgactcgtt gtaatgattc atatgctatg tttagttgct cgtgagtaag
     4381 ctcttgccct cgttctctgc tttcttctaa tagttcttgc acaatatctt gttcagaata
     4441 cacgcgcctt tctattgtca tgtgttttat atgtcctttc tgttggtcca atttaaaaaa
     4501 cgcgggcaaa cagctatagc ctacgcttta ttttttggtt agttgttagt ttacagtggt
     4561 gatgtgttgt cacaccatcg tatgcatata agtatatgct catggtttag cacagtcaat
     4621 atgaaattgc tcttttgtat aatatgtatt ttcgcattgc aaatagcatt ttaataatta
     4681 ctaaaatgtt tagtaatttt gtagacaaca tacccctgtt atggtactat tgactagctt
     4741 atttatgcgc atactggctg gttcatccag atttttatgt cacacatata gcgcggtccc
     4801 gtcgtctaac gtgagagtag ccagtaggct gctcgcaaga aaaccttccg atgaaaactt
     4861 gttttcagta gcgaagtttt gaggccacag gatgtggcag gacatcagtt tgcaaagatg
     4921 ttgtgacggg ctcgctaaaa gtgagtcgat tcagaatcga acccagctgc agtataatta
     4981 agcttacatg gtcccgtcgt ctaacggtta ggacatcagg ttttcatcct ggaaacaggg
     5041 gttcgattcc cctcgggatc accaataaaa atacctcagc tagtctgggg tgtttttatt
     5101 ggttggttgt caacggcaac cggacacctg taaagggttc agataatccc cctaagcaaa
     5161 gcgacaaacg ggcggattat accctagtat tcccctcggg atcaccaaaa gcgactcaca
     5221 gtatgtgggt ctttttttag gaacgttaga tttctgatcg caaacttagt aaaacatact
     5281 gcccctaaag ccacccaggc cagcccataa gcgtctgtta taataaatac cgatgataca
     5341 gggtgattta caagccaata ctccgctaaa aatgactaat ctgaccaaac tatatggtca
     5401 atatcgtggc gttaagaata ttaatctcac actacagcac ggtgaagttt ttggcttttt
     5461 agggcctaat ggtgccggta aaagtaccac aatacgaact attttaaact ttataacgcc
     5521 ttctgaaggt tctgctacta tttatggctt agatagcgta aaagatagtg tagaaattaa
     5581 aaacaatatt gggtacttag ctggtgatat tgccctgtac gacaacatga cgggtcgcaa
     5641 tttactaaaa tttttaacgc gtctgggcaa gcaaacagat tgggcatatg tagacgaact
     5701 cgcccatagg ctcgatgcca acctggtaag acctatccat acactcagca aagggaatgt
     5761 ccaaaaaatt ggccttattc aggcatttat gcacaaacct agcttgctga ttctagacga
     5821 accaaccagt ggccttgacc cactcatgaa gcaagtattt tacgatatgg tggccgaact
     5881 aaaagctcaa ggcaaaacta tttttgtatc ttcacacgat cttacagaag ttcaaaaaat
     5941 atgtgataga gccgccttta tacgccaggg taaactcata gcgattgaag atattcaaac
     6001 cgccaaagca attaatctgc ggaaatatcg cgcccgcttt gctaaaaaag tagatgcaaa
     6061 gccctttgtg catcttgcaa acgtctctga tgtagaagta gcaggtgacg agcttacgct
     6121 taccgtcaca gggagtatag aagcttttat tacagagctc gctaagcata aaccacaaga
     6181 tctcaatgaa caagaagtag ccctagaaga tttatttatt cattactata aggatgagtc
     6241 agacaataat gcgtaatact atctacagca aagctgtgtg ggaccgtaaa attagcactg
     6301 gtgtatggag tataagcatg gcagcttttg cacttatgtt tgcatgggtt tttgaaatat
     6361 acgcaggcga aataaccaat ttttctgata attttccagc ggaactttct gcagtaatag
     6421 gtgatcttgc agctgctaca accccagctg gatttttggc agtagaacta tattcgctct
     6481 ttttaccgtt aattgtagcg atagtaggca taacttttgg cgccgcagca attggcaaag
     6541 aagaagaatc aggcacatta gagctgttac tggcgagccc aatcagccgt cgtaaaatat
     6601 tagtgcaaaa atttggcgct atagcaaccg tactttttgt tattccgttc gccacatggc
     6661 tggggattgt agtaggaaaa agtatcttcc cgtttgacgt aaacgtatgg cacgttgcgc
     6721 tcgcatcatt tgccagtttt ttccttggca tggtgtatgc atcagcagca ttcgctgggc
     6781 agtctgtaag tggcaaacgc ggtattgggc ttggtatcgg tggtgggtta cttgcagtta
     6841 cctatattgc agatgttgta tcaaagttag tagaccgctt agaatttttg caatatataa
     6901 gcccttttta ttattttgat ataagcaatg tgctatttgg taatgggcgt atcggtaatt
     6961 ttgcagtact cgcaggtcta gtagcggtgt tttatacagt tgtacatatc gcctttaaaa
     7021 atagagatac aggcgtatag ccaataccca cttaaactat agctaataca ctgttagcac
     7081 ataccataca tcttctgtac tattgcattt tgccatagcc agcccaacct ttgcgcaaaa
     7141 atttggccag gttacattag catggttact atttttaacc atgtacaacc actgcgtaca
     7201 atttttttgt gtacggaaaa gtgattgttt ttgatatatt gtttgtttag ctagcagcgg
     7261 tcagccataa tcgctatata gtagctagtt ctatataagc atattttgca aaatgatgcc
     7321 taggttatcc acaccaaaat cccctgctcg gaccggcagg ggatttttag tttaggggtg
     7381 tgtttgtgta tgtagggggc tataagccaa gcttgcggct aatccatagt ctgtgaccaa
     7441 aggcgcctaa gatgcttact ccactcatga ttccgataac cgaacctgca cctgtattag
     7501 gtagttctgg tgttggtgtt acgggtgttt cggtacattt agggtcgtca aagttcagct
     7561 ctgggttggt tgggcatggt ttacagtttg gatcatctat tggcaaacta gggttggttg
     7621 ggcaagcaac tggtacttct gcaaacgata cggtttgggt acagtttaca ccagatactg
     7681 ttacgtcttt gccacctacc ttacctgtca cacttgcaac gatcttaaag tcgccatagg
     7741 ttgtgtaggt gtgttcagca gtttgtttgt ttactgcttt tgatccgtca ccaaagttga
     7801 tgctgtatga atcaattttg gtattttctg cagtaccttt aacagttacc tttacatact
     7861 tcttttcttt gttaacagcg tctaaggtaa gtgttgtaca tttaatagct tgatgttctg
     7921 gtttgggttc tggtttgggt tccggtgttg gaggtattac aaactctact gatttagtac
     7981 agccagcgct tgttactgtt ttggtcttat cgttaacctt accagataca tgtgccacaa
     8041 ttgtatattt gccgtcagct gcatatgtgt ggttcgcgga ctgcttgttc acaacggtgc
     8101 catcaccaaa gtctattttg taaccatcta tcttggtgtt tgatgcactg ccgctaacac
     8161 ttgcggatac tgatttcttg gttgtatcta gcgttacctt aagtgcgtca cagctaattg
     8221 cttgtacctt aggttttggt ggctctacag ccttaccagt cactgggtta ccacaaggtg
     8281 tcattacggc aaacataaat ttaccatttt tatctaacgc taccttagca gcttggg
//
LOCUS       DAOPLS010000009         8280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_9161_length_8280_cov_4.568267, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000009 DAOPLS010000000
VERSION     DAOPLS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9161_length_8280_cov_4.568267"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKV52_00480"
     CDS             complement(<1..276)
                     /locus_tag="PKV52_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPD98721.1"
                     /translation="MKHLVIVESPAKAKTIAKYLGKDYDVKSSIGHIRDLPAKGLNID
                     IKQNFAPHYEVPPEKKKTVAELKKLSKSADHIWLASDEDREGEAIAWH"
     gene            complement(633..1487)
                     /gene="dprA"
                     /locus_tag="PKV52_00485"
     CDS             complement(633..1487)
                     /gene="dprA"
                     /locus_tag="PKV52_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPD98722.1"
                     /translation="MLILSEKNQSYPSQLSRLHTPPKQLYIESHNWPEILTMPMLAVV
                     GSRKPSPYGVSNTTQIVREVASKNICIVSGLALGTDSIAHKAALEVGGRTIAVLPSGL
                     ENVYPASHRNLAKDIVASGGALISEYTPKTNASYKGNFIQRNRIIAGLSNLIFIPEAA
                     IKSGSLHTAQFAIDSGIDICAMPGHITNPLAAGCHNLIRSGAAIITGADDILNMLGIS
                     ASERTMPMAANESEKTLLDLIARGVHEAEILLDTSKLPVQTFHQTLTMLEITGKIKPG
                     GANSWFLA"
     gene            1872..2474
                     /locus_tag="PKV52_00490"
     CDS             1872..2474
                     /locus_tag="PKV52_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98723.1"
                     /translation="MHSIRKLILPELSEVILYIMSAFTLLLVANMQLLWQQNSDGVSI
                     SAVWQSQPYSYLIADQITSFEANIDPRIIDFMIWATFGALTILIFFAAQHVTKEYTER
                     IALAQSIHNKTFRKTEFTALFKKIGIQLCGLVLFVMVLRTFFGYIFPSVSNDFVTALL
                     NVTNAMSFIIISKAVAITAIGLYAIALSLRVLLLRTRVFG"
     gene            complement(2471..3118)
                     /gene="pth"
                     /locus_tag="PKV52_00495"
     CDS             complement(2471..3118)
                     /gene="pth"
                     /locus_tag="PKV52_00495"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPD98724.1"
                     /translation="MGLFQRQPLHQSLKVPYTIGVAQTRRILIVGLGNPGKKYERTRH
                     NIGFLVLDSLAASLDFPSFTTNKDFKADISEHTIGDTKVILAKPTTYMNESGQAVQRI
                     AHFYKIPLQDIVVIHDELSITFGQIRMRVGGQSAGHNGVQSVIQQLGPEFGRIRIGIK
                     NEHTPKDDTSSFVLGTFSAQEKPHVASLVNEASLVANEYIYGGTLPHDTRSIILL"
     gene            complement(3121..4470)
                     /gene="der"
                     /locus_tag="PKV52_00500"
     CDS             complement(3121..4470)
                     /gene="der"
                     /locus_tag="PKV52_00500"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPD98725.1"
                     /translation="MSTNKTPIVALVGRANVGKSSLFNVMVGYREAIVAKEAGTTRDS
                     ISVKASFDGHDFWLVDTAGIKPAEDDFELTIQEQINQAADSADVILVTVSADVQITQD
                     DRRVATMALKSKKEVILVVNKIDKAPHDDLTRWQRLGIKTIFGTSTTQRAGIEELMEY
                     LAEILPRATIKEADDRIRLAILGRPNVGKSYLFNTLAKKQQAVVADRAGTTRDINRTV
                     IRYQGKEIELLDTAGIRRSGRIERGVEQFSVLRSLAAIEQADICLLLVDVNELSVALD
                     QKIAGMINDAGKGLILVVSKWDSVEEKDAYTRDALAPQIKRDFDFIPWAPLIFTSATT
                     GKNVTKLFDLALDITAARQKKIATPELNRWLRFCADAHPPAGLKNRHPKLNYIVQTDA
                     QTPTFKVFGAHTKLLHWSYKRYLEKQLRLSFGFEGTAIKMYYLEKSIDRHKTEGKKH"
     gene            complement(4602..5270)
                     /locus_tag="PKV52_00505"
     CDS             complement(4602..5270)
                     /locus_tag="PKV52_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPD98726.1"
                     /translation="MRINKYVALATGLSRRKADAAISQGEVTVNDTIADIGVQVEKGD
                     IVALRGKHIVVPHTFTTIMLHKPIGYVCSRDGQGCKTIYDLLPATYQNLNPIGRLDKD
                     STGLLLLTNNGTLSQSLMHPSSNKTKVYMVTLDKQLLQEHAQLISGEGVLLEDGMSRL
                     ELKQYTPRDATCWWVSMQEGRNRQIRRTFSALGYTVIRLHRTQFGEYQLKNLAIGKYK
                     LVTI"
     gene            complement(5347..6849)
                     /locus_tag="PKV52_00510"
     CDS             complement(5347..6849)
                     /locus_tag="PKV52_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD98727.1"
                     /translation="MAKILLVEDDNNLREIYGERLMAEGYDIVSASDGEEALQTAVTE
                     KPDLIISDVMMPKISGFDMLDILRQTPETKDVKIIMMTALSQAEDKSRADELGADKYL
                     VKSQVTLEDVARVVHDLLGDETGELSPPAEETTPTETTASLPTNEAPVAEPMPAPAPV
                     VSEPTTVALPVEPAAEAIPSVAPTDAPSQDTQIVTAPVTEPMPAPAPVVSEPSTTAPL
                     PQQTTSVVPTVPQQASASSTNTPDPVVTTTTDPATGMGNPPVVTASAPVNPISGANPE
                     PTSPAPTVAAADPEAMQAELNEVAQQIEEFASDNATPAASHQEPVHTPTQQDIIHAQN
                     AAEEPTIVSAQPATTQANVPPVVPINTSSPEPASARKKVIQPVNGQTLSMPDIHALYE
                     EEMAKEAADSPVINPGSGSVIESPPVANDTQDNPITTAPAIEENAPPLETADVTQIEG
                     MYANEEAAEEAAAQNIPAAPLQAAPPNPSVTAQAPESTPQNPNDPNNIAL"
     gene            complement(6928..8208)
                     /locus_tag="PKV52_00515"
     CDS             complement(6928..8208)
                     /locus_tag="PKV52_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD98728.1"
                     /translation="MLGFNKKQTPQSNQAPTLAKNLFDEKAKSDIILNAIDDGVILID
                     ANKTIQLFNPAAEKITGWAQKDALNIQYTSVLKLIDKKNEPYTQEMDPFYRIFTEIAT
                     IKDNQANILSSGGKLIALDITVTPLINQQNQITGAVGVFRDVTEQRSAEAQRAEFIST
                     ASHEMRTPVAAIEGYLALAMNERVSKIDSKARDYLEKAHTSTQHLGKLFQDLLTSAKA
                     EDGRLQNHPVVTEMGEYLEQLTQDLRFSAEKKGLGMEYLIGAVGQATSGNRGEAGGKK
                     VVRPLYYAHIDPDRMREVITNLFDNATKYTDSGKVSVGITGDDHVVQIRIQDTGAGIP
                     KEDVPHLFEKFYRVDNTATRTIGGTGLGLFICRKIVEMYNGRVWVESTLGQGSIFYIN
                     LPRLTTEKAQALQIQETAAASGTAGQPQVTQPTL"
BASE COUNT         2025 a         1771 c         1856 g         2628 t
ORIGIN      
        1 gtgccacgca atggcttcac cttcgcggtc ttcatcactt gccagccaaa tatgatcggc
       61 agattttgat aactttttta gttctgctac agttttcttt ttttctggtg gtacttcata
      121 atgtggggca aagttttgct tgatatcaat attaagccct ttagctggta aatctctaat
      181 atgtccaata ctacttttta cgtcataatc tttgccgagg tacttggcaa ttgttttagc
      241 tttagctggt gattctacga taacgagatg cttcatatat ataatactgt gattttagta
      301 tacgcttccc ttaggctact ggtaacccgc taaaaatgca agtagccccg cccaatccta
      361 acgtactgtt agaatgggcg gggctaaaat aaatgctacg tggaccccgc taaggcaatg
      421 tagaatgcac cactgcccca tataaatagc aagatagtga agaatgcaag ggtcgcagca
      481 tcatcgtccc aaccatcgcc ttcacggttt acctcccagg ccaatttacc agttgcaaag
      541 gatgcaacgt agaggatgat tccaacaaag agcaaaagta acatagccac tcccacgatg
      601 agccaaattg ggatatgtta actataccac ttttatgcca aaaaccaaga attagcccca
      661 cccggcttta ttttgccggt gatttctaac atagtaaggg tttggtgaaa tgtttgcaca
      721 ggtagcttag aagtatctag caatatttcg gcttcgtgga ctccacgagc aatcagatca
      781 agtaaggttt tttcggattc gttcgccgcc ataggcatcg tgcgttctga agcggatatc
      841 ccaagcatat tgagaatatc atctgcgccc gtgataattg ctgcaccgct gcggatgagg
      901 ttatgacagc cagctgctaa ggggttagtt atatgcccgg gcatcgcaca gatatcaatt
      961 ccgctatcta tagcaaactg ggctgtatgg aggctgccgc tcttaatggc cgcttcaggt
     1021 ataaatataa ggttactaag ccctgcgata atacgatttc tttgtataaa attcccttta
     1081 tacgaagcgt tggtttttgg tgtatattca cttatcagcg ccccaccact ggcgacaata
     1141 tcttttgcta aattacgatg actcgcagga taaacattct caagaccaga cggtaatacc
     1201 gcaattgtac gtccacccac ctcgagagct gctttgtggg caatgctatc tgtgcctaat
     1261 gctaagccac ttacaatgca tatatttttt gatgcaactt cacgcacgat ttgagtcgta
     1321 ttgctcacac cgtagggcga aggttttcta ctccctacta ctgcgagcat cggcattgta
     1381 agaatttctg gccaattgtg actctctata tacagttgtt ttggtggtgt gtgcaaccga
     1441 ctaagttgcg aaggatagga ctggtttttt tctgatagta taagcataag caaataatta
     1501 ataaaatagt attgacataa cagcaaggaa gtgctaatat aagttactgt aagcgttatt
     1561 tttatgacgc attacgcacc ttataccccc aaattctaac actattgtta gtagatgtta
     1621 gaaagtttta gaacgtgccg agtaattgtt gttaccctcc acggttattt agcgctgttg
     1681 gttttaccaa cttatcatac gttctaaaac ttactaacgc ctttaaccta atgaagctca
     1741 acccctcgga tttgtgtgag gtgggaaaat gtagcgtgtg ggacacactc cccgggttga
     1801 aggctgaaaa gagcctacag cggtgcccac cccctctgta ggctcttttt tgttatactt
     1861 atgtttatat tatgcactct attagaaaac ttatcttgcc agagcttagt gaagtaatat
     1921 tatatatcat gagtgcgttc acactactgt tagtagccaa tatgcagcta ttatggcagc
     1981 aaaatagcga tggggtgagc atatctgctg tctggcaatc acagccatat agctacctca
     2041 ttgctgatca aattacatct tttgaggcaa atattgatcc acgtattatt gattttatga
     2101 tatgggcaac ttttggtgca ctcaccatat tgatattctt tgctgcgcag catgtcacta
     2161 aagaatatac agaaagaatt gccttggcgc agtctataca caataaaaca tttcgtaaaa
     2221 cagagtttac tgcactattc aaaaaaattg gtatacagct ctgtgggtta gtactctttg
     2281 tcatggtact taggactttt tttggatata ttttcccatc ggtctcaaat gattttgtga
     2341 ctgctttact caatgtaaca aacgcgatga gctttataat aattagtaaa gccgtagcaa
     2401 taacggcaat cggtttatac gccatagcac ttagtctacg agtgctactt ttacgcaccc
     2461 gagtgtttgg ttaaagcaat ataatagaac gagtgtcgtg tggtaaagtt ccaccgtaaa
     2521 tgtactcgtt tgcgacgagc gacgcttcgt ttaccagcga cgctacgtgt ggtttttctt
     2581 gtgcagaaaa tgtaccaagc acaaagctac ttgtatcgtc ttttggtgta tgttcgtttt
     2641 taatgccaat gcgaatacgt ccaaattctg gtccgagctg ttgaatgacc gattgtactc
     2701 cattgtggcc agctgactgc ccacctacgc gcatgcggat ttgaccgaac gttatagaca
     2761 gctcatcgtg tatgactaca atatcttgca gcgggatttt ataaaagtga gcaatgcgtt
     2821 gtacggcttg acctgattcg ttcatgtacg tggtaggttt tgcgagaatt accttagtgt
     2881 caccaatagt atgttcggaa atgtcagcct taaaatcttt gtttgtggta aagcttggaa
     2941 aatctaatga cgcagcgagc gaatcaagta ccaaaaaccc aatattgtgc cgcgtacgtt
     3001 catatttttt acctgggttc ccgagcccaa caatgagaat gcgacgtgtc tgagctacgc
     3061 ctatagtgta gggcactttt aatgattggt ggagtggctg tctttgaaat aagcccattg
     3121 ttagtgtttc ttcccttctg ttttatgtcg atcgatactt ttttctaaat agtacatttt
     3181 tatcgcagtt ccttcaaagc caaagcttaa acggagttgt ttttctaggt agcgtttata
     3241 gctccagtgc aggagtttgg tatgggcacc aaagacctta aatgtaggtg tttgggcatc
     3301 tgtttggaca atatagttga gcttcgggtg ccgattcttt agccctgctg gaggatgcgc
     3361 atcagcacaa aaacgaagcc atctattaag ctctggggta gctatctttt tctggcgagc
     3421 agcggtgata tctagtgcta aatcaaatag tttcgtaacg tttttacctg ttgtcgcgct
     3481 ggtaaatatt aggggagccc acggtataaa gtcaaaatca cgttttattt gcggagcgag
     3541 tgcatcacgt gtgtaagcat ctttttcttc tacactatcc catttgctta ccactaaaat
     3601 tagccccttc cctgcgtcgt ttatcatgcc agcgattttt tgatcgagcg caacacttag
     3661 ttcgtttacg tctacgagca gtaagcaaat atcagcttgt tcaatcgcag caagagagcg
     3721 caacacacta aactgttcga ctcctcgttc gatccgccca ctgcggcgaa taccagctgt
     3781 gtctagaagt tcgatctctt taccttggta ccgtatcact gtacgattga tgtctcttgt
     3841 ggtgccagcc ctatctgcaa cgaccgcttg ttgttttttt gccagagtat taaaaaggta
     3901 gctcttgccc acatttggtc gccctaaaat tgctaatcga atgcgatcat cagcttcttt
     3961 aatagttgcc cttggtaata tttcggccag gtattccatg agctcttcta tgccagctcg
     4021 ttgggttgtg ctcgtaccaa atatggtttt aataccgagt cgttgccaac gtgttaggtc
     4081 gtcgtgtggt gctttgtcaa ttttattaac aaccagaata acttcttttt tgcttttaag
     4141 agccatagtg gcaacgcggc ggtcgtcttg agtgatttgt acgtctgcag aaacggttac
     4201 aagtataaca tctgcactat cggctgcttg gttaatttgt tcttgtatgg tgagctcaaa
     4261 gtcgtcttct gctggtttga tacctgcagt gtctacgagc caaaaatcat gtccatcaaa
     4321 actagctttc actgaaatac tatcacgtgt agtgccagct tcttttgcaa caatagcctc
     4381 gcggtagcca accataacat taaacagact gcttttgccc acgttggcac gccctacaag
     4441 ggcaacaatt ggtgttttgt ttgtactcat tgccatcagt ataacagata tagactcatt
     4501 gacaaataga cgtattgcat gcataactac atatgatgaa cagcatagat attttgagtt
     4561 atacgctacg catacatatg tagcgtttgt tgcgcgacgt gttatatagt aacgagttta
     4621 tatttaccaa ttgctaaatt tttgagttgg tattcaccaa actgagtccg gtgcagacgt
     4681 atcacagtgt agcctaacgc agaaaacgta cgacgaatct gacgattcct accctcttgc
     4741 atacttaccc accagcacgt ggcatcacga ggagtgtact gcttgagctc tagtctactc
     4801 atgccatctt ctaaaagtac accttcccca ctgataagct gtgcgtgttc ttgaagtagt
     4861 tgcttatcta gggtaaccat atagacttta gtcttattac tgctagggtg catcaggctt
     4921 tgtgagagcg taccattgtt tgtcaaaagt aataacccag tcgaatcttt atcaagccga
     4981 ccaatcgggt taagattctg gtaagttgca ggcagtaggt cataaatagt tttacatcct
     5041 tgaccatctc gactgcatac gtagccaata ggtttatgta gcattatcgt tgtgaaggtg
     5101 tgtggtacga cgatgtgttt gccacgtaga gcgacaatgt cacctttttc tacttgcaca
     5161 ccaatatcag caatagtgtc gtttactgta acttcacctt ggctaatcgc agcatctgct
     5221 ttgcgccggc tgagcccagt ggcgagcgct acatacttat taatacgcat atctatatac
     5281 taccagtgta tggcaaggta tcactagcga taaggttcat gtgtagccca ggtgtttgtc
     5341 gttgttttac agtgcaatat tgtttgggtc gttgggattt tgaggtgtgg actctggagc
     5401 ttgagctgta acactaggat taggtggtgc tgcttgcagc ggggcagctg ggatattttg
     5461 ggcggcagct tcttctgcag cttcttcatt agcgtacatg ccttctattt gtgtaacatc
     5521 agctgtttct aatgggggtg cgttttcctc gatggcaggt gctgttgtaa ttggattatc
     5581 ttgggtatcg tttgctaccg gcggactttc gatgacacta ccgctgcccg ggttaataac
     5641 cggagaatca gcagcctctt tagccatttc ttcttcgtat aaggcatgaa tatctggcat
     5701 tgacagcgtt tggccattaa ctggctgaat aacttttttt ctggcagatg ctggttctgg
     5761 actacttgtg ttaattggta caacaggagg tacgtttgct tgggtggttg cgggttgtgc
     5821 gcttacaata gttggctctt cggcagcatt ttgagcatgg ataatatctt gctgagtggg
     5881 tgtatgcaca ggttcttggt gtgatgctgc tggtgtcgca ttatctgacg cgaactcttc
     5941 gatttgctgg gcgacttcgt taagttctgc ttgcatagct tctggatcgg cggctgcgac
     6001 agttggtgct ggcgacgttg gttcaggatt tgcgccactt atagggttaa cgggcgctga
     6061 agctgtgacc actggtggat tacccatacc agtagctgga tcagtcgtag tggtaactac
     6121 cggatctggc gtatttgtag agcttgcact ggcttgctgc ggcactgtcg gcactacaga
     6181 agtagtttgc tgcggcaatg gtgctgtagt gctaggctca ctcacaacgg gtgcaggagc
     6241 tggcatgggc tcagtcactg gcgcggtgac aatctgtgtg tcttggctcg gcgcatctgt
     6301 tggcgcaaca gaaggtattg cttctgctgc aggttctact ggcagtgcta ccgtagtagg
     6361 ctcactcaca acgggtgcag gagctggcat gggctcggct actggcgctt catttgttgg
     6421 taggctggca gtagtttcag ttggagtcgt ctcttctgca gggggcgata attcgccagt
     6481 ttcgtcacca agtaagtcat gaactacgcg agctacatct tctagcgtga cttgcgattt
     6541 tacaagatat ttatccgcac caagttcatc tgcgcgtgat ttatcttctg cctgactaag
     6601 ggcagtcatc attataattt taacgtcttt tgtttctggc gtttgccgta agatatctag
     6661 catatcaaag ccacttatct ttggcatcat aacatcacta ataataaggt ctggcttttc
     6721 agttacggca gtttgtaatg cctcttcgcc atcgcttgca gacacgatat catagccttc
     6781 agccatgagc cgttcgccgt atatttctct taggttgtta tcgtcttcta ccagtaggat
     6841 ttttgccatg atatgtaagt tgccctttat tgattatgct tatctgatta ttgtagcatg
     6901 gagctgattt cttttactag ggtgcgacta caaggtaggt tgcgtcactt gtggttgccc
     6961 agcagtacca cttgcggcag cggtttcttg gatctggagc gcttgggctt tttctgtcgt
     7021 tagtcgcggc aaattgatat aaaagatgct tccctggcca agtgtgcttt ctacccatac
     7081 tcgcccgtta tacatttcta caattttgcg gcaaataaac aaaccgagac cagtaccacc
     7141 tatggtgcgt gtcgccgtat tgtctacgcg gtaaaacttt tcaaacaagt gtggtacatc
     7201 ttctttggga ataccagcgc cagtgtcttg gattctaatt tgaacaacat ggtcatcacc
     7261 agtaatgccc acgcttactt ttccgctatc tgtatatttt gtggcattat caaacaagtt
     7321 ggtaatcact tctctcatgc ggtctgggtc tatgtgagca taataaaggg gccttacaac
     7381 ttttttcccg ccagcttcac cgcggttacc gctagtggct tggccgacag cgcctataag
     7441 gtattccata ccaagaccct ttttttctgc actaaagcgc aaatcttgtg ttaattgctc
     7501 taaatattca cccatttctg tcactacagg gtgattttgc aaacggccgt cttctgcttt
     7561 ggcacttgtt aataaatctt ggaataactt acccaagtgc tgggtgctgg tgtgagcttt
     7621 ttctaaataa tcacgtgcct ttgagtctat tttagagacg cgctcattca tagcaagcgc
     7681 taagtaacct tctatcgcag ctactggtgt gcgcatttcg tggctggcgg tacttataaa
     7741 ttctgcccgt tgtgcttctg cgcttcgttg ttcggtgacg tctctaaata cgccaactgc
     7801 gcccgtgatc tggttttgtt gattgataag tggggtgact gtaatatcaa gcgcgatgag
     7861 cttgccaccg cttgataata tattagcttg attgtctttg atggtagcaa tttctgtaaa
     7921 aatacggtaa aacggatcca tttcttgcgt gtatggttcg ttctttttat ctatgagctt
     7981 taatacagat gtgtactgaa tattaagtgc atctttttgt gcccaccctg taattttttc
     8041 tgctgctggg ttgaaaagct gaattgtttt attagcatca attaaaataa cgccgtcgtc
     8101 tatggcattt aaaatgatgt cagatttggc cttttcgtca aataaatttt tggcgagcgt
     8161 aggcgcttgg tttgactgtg gcgtttgctt tttattaaaa ccgagcatta ttgcagcaca
     8221 atcccaccat tgctagtaaa acgcatacgt ttattattgc agttacaccc tgtaaaaaca
//
LOCUS       DAOPLS010000010         8222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_9258_length_8222_cov_4.757806, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000010 DAOPLS010000000
VERSION     DAOPLS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9258_length_8222_cov_4.757806"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /gene="rplU"
                     /locus_tag="PKV52_00520"
     CDS             complement(<1..177)
                     /gene="rplU"
                     /locus_tag="PKV52_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPD98729.1"
                     /translation="MKKAVITTGGKQYIVAEGDTLQVELLSEDKTVTFEPLLVIDGDK
                     TLVGTPVVTGAKVTA"
     gene            complement(260..3139)
                     /gene="ileS"
                     /locus_tag="PKV52_00525"
     CDS             complement(260..3139)
                     /gene="ileS"
                     /locus_tag="PKV52_00525"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPD98730.1"
                     /translation="MKFKKEQRKKPAEYEKEVVEYWKQNDTFNKSVANRPDKDRWVFY
                     DGPPFLTGTPHHGHLLISAVKDSVARYQTMKGKHVERRWGWDCHGLPAEVFVEKQLGI
                     TNKKDIGVKISIPDYVNACRASMVQTGTEWEDTVDRIGRWVEFKGAYKTMDNEYMESV
                     WWAFKELYEKGKIYEGEKILVYCTKDATPISKSEVAMENSYQVDTDPSVYVYFKLEET
                     DEHLLAWTTTPWTLPANVAIALHSKLTYALVEYDGKKVYVADAALERVFKDEKKQPLA
                     YKVLKKVKGSELVGKRYHPLFENHGPEAHRVLHAEFVTAEDGTGIVHEAPAYGEDDYE
                     LCKTAGIPIVSMVDENGSYTSGRWQEQNIWAVNKEIAKTLVAEGSAMKVEYIQHEYPH
                     CHRCGSKLMYRAHPSWFMDIQDQKQEMLVANSKTSWTPSTLRDGRFANIIDSAPDWNL
                     SRDRYWATPIPVWKGQRPDGTEVIKVVGSYAEFKELTGKSLSDYHLPNVMDVTFTVDG
                     VELKHIGKVIDCWFESGSMPFAQFHYPFENVAKFKESFPADFIIEAIDQTRGWFYSLM
                     AVNVGLFGKAPFKNLICTGFINAADGKKMSKKLKNYTDPNELMDAYGADSFRFLLLGS
                     PLLNGEDFSLKDKEVLDVQRKLAMVWNMYDFFTLYAEVDKWEYNPKNKPEISKLKNLL
                     DIWIVSRLHQLSIEVDKSFGDYDLPGAIKPILPFIDDASNWFVRRSRKRFWKTDNDTD
                     KQDAYATLHYVLMTLSRIMAPITPFMSEALYRNLTGKESVHLTDWPHFQPIDTSVIEQ
                     MAQIRELITIGLSQRADAGIKVRQPLASAQVNTSVQIPSHLADAYTAILQEELNVKTV
                     VLQHGKEPKVTIDTTLTDELLAEGMVRDVVRQVQSARKKADFNVEDRILCELSTPDKK
                     LSQAIEHHQAYISKETLSSPHVLQNPMYKDSVTIGGATLTIALGK"
     gene            complement(3388..3660)
                     /locus_tag="PKV52_00530"
     CDS             complement(3388..3660)
                     /locus_tag="PKV52_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98731.1"
                     /translation="MKVAVLYRSNSEHERLVSNFEREFEYRTGRSFVKYDVNTRDGAA
                     MASLYDVMRYPSVLALADDGQMLQMWQGDTLPLINEVTYYSPAMAG"
     gene            complement(3679..6435)
                     /locus_tag="PKV52_00535"
     CDS             complement(3679..6435)
                     /locus_tag="PKV52_00535"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPD98732.1"
                     /translation="MAETTTLGLGRYFTRSGKKAYDQLTWVKRDSEIINPMTGEAVFE
                     QKGVEFPEDWSLNAINIVAQKYFAGTPGEADREDGLKVLIDRIVDTITRAGVQEDYFI
                     DDKEAESFREELKFILATQRAAFNSPVWFNIGVPSRAQQASACFILGVEDSMPSILNW
                     YVEEGMIFKGGSGAGINLSNLRSSAESLSASGGKASGPVSFMRGADASAGTIKSGGKT
                     RRAAKMVILNADHPDIEEFIWTKAIEERKARALQEAGFDMSLNGKDNFSVQYQNANNS
                     VRVTDEFMQAVEADADWDLKAVTTGETVKTVRARDIFRQFAEAAWECADPGMQYDTTI
                     NHWHTSPNAGRINGSNPCSEYMHLDNSACNLASINLMKYIKDDNTFDVEAFKHTVEVV
                     FTAQEILVGYSSYPTDSIAKNAKAYRELGMGYANIGALLMAQGLPYDSEEGRAQAAAI
                     TAIMTGHAYATSARLARRVGPFAGYHKDKEAMTRVLRQHRAEVDKIDARRVAEDLLSS
                     AAETWDEAIELAEIHGVRNSQATVLAPTGTIGLMMDCDTTGIEPDLSLVKHKKLVGGG
                     TMSIVNRTVPRALRELGYTKAQTDEIVAYIDEEKTIVGAPHLKKEHLPVFACSMGDNT
                     IHYLGHVKMMAAVQPFISGAISKTVNMPEEATVEDIEQLHIDAWKMGVKAVAIYRDNC
                     KVAQPLSTAKKDSAQEQPADSLQATKDAYIVKSAMKRELPKRRNSKTYEFRVADAKGF
                     FTVGEYEDGKPGELFIHVAKQGSTLSGLMDSFAISVSHGLQYGVPLKSYVKTMMSRSY
                     APSGITDDPQIRTATSITDYIFKRLGHDYLSFDDKIELGLASIDDIDDLQQASLLDTP
                     EPQSEETIVSKSTDEVQPTPAQSTKKVAKDSTAPLCYNCGNQTQRSGSCYVCTACGST
                     TGCS"
     gene            complement(6638..7114)
                     /gene="nrdR"
                     /locus_tag="PKV52_00540"
     CDS             complement(6638..7114)
                     /gene="nrdR"
                     /locus_tag="PKV52_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPD98733.1"
                     /translation="MKCHNCNFADTKVIESREVADGESTRRRRKCPHCDARFTTYERR
                     ERPQLVVIKQDNSRQLFDRNKLLAGLHRACEKTPVTNLQLEQLVANIENKLASQGDPE
                     VSSRAIGELVMTELANLSEVAYVRFASVYRRFDDITGFEAELAQIRAKKNVADTAP"
     gene            complement(7249..>8222)
                     /locus_tag="PKV52_00545"
     CDS             complement(7249..>8222)
                     /locus_tag="PKV52_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013191011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPD98734.1"
                     /translation="KLRTEVDTLITVPNDRLLQTIDRKTPLLDAFKVADDVLRQGVQG
                     ISDLITVHGLINLDFADVRAVMSNAGSALMGIGRASGDDRATEAAHQAIESPLLEVSI
                     DGARGILFNVIGGLDMSMHEINAAAETITAAVDPEANIIFGATINPELEGEVIITVVA
                     TGFEASYFANRQQIRETQAPLDAAPSQDAPLVATPVSEVKASPEPTTDDTKVSLGDDT
                     SEETEKDDSKKLETVSDTKTMTEEDIKDINLDVDDKPHTASDFTDDKPVPNIWTFDAD
                     HKDEDEDKFDKPSFLRRFTRRGKKQQDEDDDDEKTDNKDDDKSADKK"
BASE COUNT         1990 a         1936 c         1645 g         2651 t
ORIGIN      
        1 tgcagtaact ttagcgcctg ttactactgg agtaccaacg agtgttttgt caccgtcaat
       61 caccaatagt ggctcaaatg tcacggtctt atcttcagaa agtagttcta cttgcaacgt
      121 gtcgccttct gccacgatat attgcttacc accggtggtg attacagctt ttttcattac
      181 tatcctctta tcatttatga attagtcaac tgatatggta ccagatatag ctcttttagt
      241 aaagagtagc tagggagaac tatttcccca aggctattgt tagtgttgca ccacctattg
      301 taacgctgtc cttgtacata gggttttgta atacgtgtgg tgatgacagc gtctctttag
      361 atatataagc ttgatgatgc tcaatagcct gggataactt cttatcagga gtacttagct
      421 cacataaaat tctatcttct acattaaaat ctgctttttt acgggctgat tgcacctgcc
      481 tgacgacgtc tcgtaccatg ccctcggcta acaattcatc tgttagagtg gtatcaatcg
      541 ttacctttgg ctctttacca tgctgtaaca ctactgtttt aacgtttagc tcctcttgta
      601 agattgctgt gtatgcatct gctaggtgtg acggtatttg tacagatgtg tttacctgtg
      661 cggatgctaa tggttgtctt acttttatgc ctgcatctgc acgttgtgat agccctatgg
      721 ttatgagttc ccttatctga gccatttgtt caataacaga tgtatctata ggctgaaaat
      781 gtggccagtc agtgaggtga accgactctt taccagtgag gtttctgtat agcgcttcgc
      841 tcataaacgg tgtgattgga gccataatac ggctaagtgt cattaatacg tagtgcaagg
      901 tcgcataggc atcttgtttg tcggtgtcgt tgtctgtctt ccagaatcgt ttacgactac
      961 ggcggacaaa ccagttactg gcatcatcta taaacggtag tatgggtttg attgcacctg
     1021 gcaaatcgta gtcgccaaag ctcttgtcta cttctataga gagttggtgt aacctagata
     1081 ctatccatat atctaagagg ttctttaatt ttgatatttc tggtttattc tttggattat
     1141 attcccattt atctacttct gcatacaatg taaagaaatc atacatattc caaaccatgg
     1201 caagtttgcg ctgcacgtct aaaacttctt tatctttaag tgagaagtct tcgccgttaa
     1261 gtagcggtga accgagtaac aagaaccgaa agctatcagc tccgtatgca tccatgagtt
     1321 cgtttggatc ggtatagttt ttgagttttt tactcatctt tttaccatcc gctgcattta
     1381 taaagcctgt acatattagg ttcttaaatg gcgccttacc aaataaacct acgttgaccg
     1441 ccattagtga gtaaaaccag ccacgtgtct ggtcaatagc ctctataata aagtcggccg
     1501 gaaaactttc tttaaacttt gcgacgtttt caaaaggata atggaactgt gcaaaaggca
     1561 tactaccact ttcgaaccaa cagtctatca ccttaccaat gtgctttagt tctacgccat
     1621 ctactgtaaa cgttacgtcc atgacatttg gcaaatgata atcgctaagg cttttacctg
     1681 ttaactcttt aaattctgcg taactgccta caaccttaat aacctctgta ccatcgggcc
     1741 tttggccttt ccaaactggt attggagtag cccagtatct atcgcggctt aagttccaat
     1801 ctggagcaga atctataatg tttgcaaagc ggccatccct taaggtgctt ggtgtccagc
     1861 tcgttttact atttgccaca agcatttctt gtttttgatc ttggatatcc ataaaccaac
     1921 tcggatgtgc ccggtacatt aatttagacc cacagcgatg gcagtgtggg tattcatgct
     1981 gtatatactc aaccttcatt gcagaacctt cggcgaccaa agtcttggca atttctttgt
     2041 ttacggccca aatgttttgt tcttgccaac ggccagacgt ataactaccg ttttcgtcta
     2101 ccattgacac tattggaata ccagctgttt tacacagctc gtaatcatct tcaccatacg
     2161 ctggtgcctc gtgtacaatg cccgttccgt cttcggcggt tacgaattct gcatgcagta
     2221 ctctgtgtgc ctctggacca tggttttcaa ataacggatg ataccgcttg ccaaccagct
     2281 cgctcccctt cacttttttt aacactttgt atgctaatgg ttgctttttt tcatctttaa
     2341 acacacgctc taaggccgca tctgctacat acactttttt gccatcgtat tccacaagtg
     2401 catatgtaag tttagaatgg agagcgattg caacattggc aggtaatgtc catggcgtag
     2461 tcgtccaggc aagtaagtgt tcatcggttt cttctagttt aaaatacaca tacacgcttg
     2521 ggtcagtatc tacttggtaa ctattttcca tggcaacttc actctttgat atcggggtgg
     2581 catcttttgt gcaatacacg agtatttttt cgccttcgta gattttgcct ttttcgtata
     2641 gctctttaaa agcccaccat accgattcca tgtattcgtt atccatggtt ttgtaagcac
     2701 ccttaaattc aacccagcgg cctattctat caacggtgtc ttcccattcc gttcctgtct
     2761 gcaccataga ggctctacac gcatttacgt agtctggtat gctaattttt acaccaatat
     2821 cttttttatt ggtaatacca agttgttttt ctacaaatac ctcagctggc aaaccatggc
     2881 aatcccaacc ccaacgacgc tcaacatgtt tacctttcat cgtttggtac cgtgcaaccg
     2941 aatctttgac tgcagagata agtagatggc catggtgtgg tgtcccagtt aagaatggcg
     3001 gaccatcata aaatacccac ctatctttat ctggtctgtt ggcgacagat ttgttaaacg
     3061 tgtcattctg tttccagtat tcaacaacct ctttttcgta ttcagctggc tttttacgtt
     3121 gctctttttt gaacttcaca ggtatccttt caaataaaaa accacccgtt gcttcggatt
     3181 ccgactattg ctagcggaag gtaccatccg aataacttgg tggagttact ttgttacaac
     3241 tacttacgtg agtttcaaac gttgcggtct agtgtagcgt tatgggctag ttcttcgcaa
     3301 tgcttcgtgg ctgataacca ctcatccgta aacggaatgc atctcataaa ttgtctgtta
     3361 ttactatatc tgttattggt cggaatttca accagccata gctggactat aataagttac
     3421 ttcattaatg agcggcagcg tgtcgccttg ccacatttgt agcatttgtc cgtcatcggc
     3481 tagcgccaac acactcggat agcgcatgac atcatataag cttgccattg cagcgccatc
     3541 acgtgtgttg acgtcgtatt taacaaaaga gcggcctgtc cgatattcaa attcacgttc
     3601 aaaattactg actaatcgtt cgtgctctga attgctccga tacaaaaccg ctactttcat
     3661 tgttccacct attctggttt aactacatcc tgttgtcgat ccgcacgctg tacatacgta
     3721 gcagctgcct gatcgctgag tttggttccc acagttatag cataacggtg cagtgctatc
     3781 tttggcaact ttttttgttg attgtgcagg tgtaggttgc acttcatctg ttgattttga
     3841 cactattgtc tcctctgatt gtggctcagg agtgtcaagt aatgaagcct gttgcagatc
     3901 atcgatatca tcgatactcg ccagccctaa ttcaatttta tcgtcaaaag acaagtagtc
     3961 atgcccaaga cgcttaaaaa tgtaatcggt tatagatgta gcagtgcgaa tttggggatc
     4021 atctgtaata ccagatgggg catacgaacg gctcatcatc gtcttgacgt aactctttag
     4081 tggcacaccg tactgtaagc catggctcac cgatatagca aacgagtcca tcaggccaga
     4141 tagtgtagat ccctgcttcg ccacatggat aaacaattca cccggcttgc cgtcttcgta
     4201 ttcgcctacc gtaaagaatc ctttggcgtc tgcaacccgg aactcatagg ttttactatt
     4261 acgccgtttt ggaagctcgc gtttcatggc acttttgacg atatatgcat ctttagtagc
     4321 ctgtaggcta tcagctggtt gttcttgggc ggaatctttt ttggccgtag aaagcggttg
     4381 tgcaaccttg cagttgtcac ggtaaattgc caccgccttc actcccattt tccacgcatc
     4441 aatatgtaat tgttcaatat cttctacggt tgcttcttct ggcatattca ctgttttgct
     4501 aattgcacca gaaataaacg gttgaaccgc agccatcatt tttacatgac ccaggtagtg
     4561 aatggtgtta tcgcccatag aacacgcaaa tactggcaaa tgctctttct ttaggtgtgg
     4621 cgcgccaaca atagtctttt cttcgtctat gtatgccaca atttcatctg tttgtgcttt
     4681 ggtatagcca agttcgcgga gtgcccgtgg taccgtacga ttaacgatac tcatcgttcc
     4741 accaccaact aattttttat gctttactaa gctaagatct ggctctatgc ctgttgtatc
     4801 acagtccatc atgagcccaa ttgttcctgt tggggctaaa acggtagctt ggctattgcg
     4861 tacgccgtga atttctgcga gttcaatggc ttcgtcccac gtttctgcag cactgcttaa
     4921 caagtcttca gcaactctgc gcgcgtcaat tttatctact tctgcgcggt gctgtctaag
     4981 cactcgtgtc attgcctctt tgtctttatg ataccctgca aatggcccaa cacggcgcgc
     5041 aagccgtgcg ctagtggcat aggcatgtcc ggtcataatg gcggtaattg cagctgcttg
     5101 cgcccgtcct tcttctgaat catatggtaa gccttgggcc ataagtaatg caccaatatt
     5161 tgcatagccc atgcctagtt cgcggtaggc cttagcgttt ttagcaatac tatctgttgg
     5221 gtaactagaa taacccacta aaatttcttg tgcagtaaat accacttcta cggtatgttt
     5281 aaacgcttct acatcaaaag tgttatcgtc tttgatgtat ttcataagat tgatacttgc
     5341 caaattacag gctgagttat ctaggtgcat gtattcacta caagggttag agccattaat
     5401 tctgccagcg tttggcgacg tatgccagtg gttaatcgtg gtgtcgtact gcataccagg
     5461 gtcggcacat tcccaggctg cctcggcaaa ttgcctaaag atatcacgtg cccgcactgt
     5521 tttgactgtt tctccggtgg taaccgcttt taaatcccag tcagcgtcag cttctactgc
     5581 ttgcataaat tcatccgtta cccgcaccga attattagca ttctgatact gcacgctaaa
     5641 gttatcttta ccgttcaagc tcatatcaaa gccagcctct tgcaaggctc gtgctttgcg
     5701 ctcttctatt gctttggtcc atataaattc ttcgatatct gggtggtcag cgttcagtat
     5761 gaccattttt gcggcccgtc ttgtcttacc accgcttttg atggtaccag cactggcatc
     5821 tgccccacgc ataaaactca caggcccgct tgccttgcca ccgctcgcac tcaatgattc
     5881 tgcactagag cgaagattag acaagttgat tccagcacct gagccaccct taaatatcat
     5941 gccttcttct acgtaccaat ttaatattga aggcatactg tcttctactc caagaataaa
     6001 gcaagcgctc gcctgctgag cgcgtgatgg tacgccaata ttaaaccaga cgggcgaatt
     6061 aaaagctgcc ctttgagtgg ctaatataaa ctttagttct tcccgaaagc tttctgcctc
     6121 tttatcgtct ataaagtaat cttcttgaac accggctctg gtaattgtat ctacaatacg
     6181 gtcaatcaat accttaaggc catcttctct gtcggcttcg cctggtgtgc ctgcaaaata
     6241 tttttgagcg acaatattaa tagcattcag cgaccaatct tctggaaact caacgccttt
     6301 ttgttcaaac accgcttcac ctgtcattgg attaataatt tcagaatccc gttttaccca
     6361 agtaagctga tcatacgctt tcttacccga acgcgtaaaa taccgcccta gccctagtgt
     6421 tgttgtttct gccatagtac taccctcact ttatgttaat tatttgatat gtttattaaa
     6481 gctcgccact ttttttgagc gaccaaatac cctcccacaa gtactcgtca ctatgttgtg
     6541 tggtgtgttc gtcttatagc gcgctttgct ttgtatctta agcctatggg cactacgcta
     6601 caaaacaaaa cgaacagcga cgattagata agtaagttta cggcgcagtg tctgctacat
     6661 tctttttcgc tcttatttgg gccagttctg cctcgaagcc agtaatatca tcaaaacgac
     6721 gataaacact cgcaaaacgg acatacgcaa cctcagaaag gttggcaagc tctgtcatga
     6781 cgagttcgcc aatagcgcga gaagagactt ctgggtcacc ttggctggca agcttgtttt
     6841 ctatgttggc cactaactgc tctagttgca aatttgttac aggagttttt tcgcacgctc
     6901 tgtgcaaccc tgccaataac ttgtttctat caaacaattg ccgagagtta tcttgtttta
     6961 tcaccacgag ctgtggacgc tctctacgtt cataggtagt aaaacgtgcg tcgcagtgag
     7021 gacacttgcg gcggcggcgc gttgattcac cgtcggccac ttccctagat tcaataactt
     7081 ttgtatcggc aaaattgcag ttatgacact tcacttggtt tccttactta tttaggcttt
     7141 aacgtacaat cgctatatat agcgtgtatg tactgtatga tatacccatt atatagcgtt
     7201 ttcaatacat ttaatgtcgt aatttagtac acagccaaat aatactagtt actttttatc
     7261 ggcagattta tcgtcgtcct tgttatcggt tttttcgtca tcatcgtctt cgtcctgctg
     7321 ttttttgcca cgacgagtaa atcgtcgcaa gaacgaaggc ttatcaaact tgtcttcatc
     7381 ttcatctttg tggtcagcat caaaagtcca aatattaggc actggcttgt cgtcggtaaa
     7441 atcactagca gtatgcggct tgtcgtctac gtctaaatta atatctttta tgtcttcttc
     7501 tgtcattgtt ttcgtatcgc tcactgtttc tagcttttta ctatcatctt tttctgtctc
     7561 ttctgatgtg tcatcaccaa gagacacttt agtatcatct gttgtgggct cgggcgatgc
     7621 ttttacttca gatactgggg tggcaaccaa tggtgcatcc tgtgatggtg cagcgtctag
     7681 aggtgcctgt gtttcacgaa tttgctggcg gttggcaaaa tatgacgctt caaagcccgt
     7741 tgcaacaacc gttataatta cttctccctc gagctctggg ttgatggttg caccaaaaat
     7801 gatgtttgcc tctgggtcaa cggcagcagt aattgtttcg gcagcagcat tgatttcgtg
     7861 catagacatg tctaagccac caatcacgtt aaataatatg ccacgagcgc catctataga
     7921 aacttctagt agtggtgatt ctatggcctg gtgagcagct tctgtggctc tgtcgtcacc
     7981 acttgccctt cctataccca ttaaggcgct accagcgttg ctcatgaccg cacggacgtc
     8041 tgcgaagtca aggttaataa gcccatgcac cgttattaaa tcagaaatac cctgcactcc
     8101 ctgtcttaaa acgtcatcag ctaccttaaa agcgtcaagc agtggtgttt tacggtcaat
     8161 cgtctgaaga agtctgtcat ttggtacggt tataagagta tctacttctg tacgaagttt
     8221 gt
//
LOCUS       DAOPLS010000011         8164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_9345_length_8164_cov_4.142804, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000011 DAOPLS010000000
VERSION     DAOPLS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9345_length_8164_cov_4.142804"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKV52_00550"
     CDS             <1..421
                     /locus_tag="PKV52_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98735.1"
                     /translation="IASTILLYALPIVGSLLEATFVYTIGALLMALSLYWLLVWWPAL
                     YIAMLPGTTPIGAMRAAAMLTKGRRFKIFSRQFAILVVLGSLFFVVVFPLAIVWQRLV
                     PLSVYVSIITLYGIGHIALFNLYRSLIDEQPTATPKN"
     gene            390..2402
                     /gene="ligA"
                     /locus_tag="PKV52_00555"
     CDS             390..2402
                     /gene="ligA"
                     /locus_tag="PKV52_00555"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPD98736.1"
                     /translation="MNSPQPRQRIKQLVELLNTYSYEYHVLDKPTVDDAIYDALHAEL
                     ENLESTYPELIEPSSPTQRIVSSPLSGFQKVEHKHRMLGLSDIFSTQELEDWLSRITK
                     IDSKVATTHFWASIKMDGLACAIIYQNGVLKTAITRGDGFVGEDITNNVKTIKSVPLA
                     LPHNHPFSDGRTELRGEIVMYKTDFAHINKQLQDAGEKVYANPRNLAAGTMRQLDAKV
                     AASRKLHFIPYDILRDDPEQISTYQFVYNTFAELGFISSSRTAKLLNSADDILAYYES
                     WKDKRHELPYNTDGLVIKVNDRQLYATLGVVGKNPRGAVALKYPAETATTILRDIFIS
                     IGRTGAATPVAVLEPVVVAGSTVQMATMHNDSEIQRKDVRIGDTVVIQKAGDIIPEVV
                     QPIKELRNGTETEFVMPVHCPECNTKLTRPTNEVVWRCINVNCPARTWRHIQHYASKA
                     ALDIEGLGEKNVQALLDAHLISDAADLYTLQKQTISTLDRFADLSASNLVEAIAKKKN
                     PPLAKFIFGLGIRHVGAQTAIELVNHYKTLEALSAASLDELQTIDGVGEVVAESIISW
                     FSDQENRKLLRKFAYNGVVPQSVTTTTQGSLQGMYIAITGSLSSMSREAAADKIRQRG
                     GTFQSSVGKNTTHLVAAGTIGSSKLAKAEKFGTKIIDEQAFLQMVQ"
     gene            complement(2409..2708)
                     /locus_tag="PKV52_00560"
     CDS             complement(2409..2708)
                     /locus_tag="PKV52_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98737.1"
                     /translation="MNDEDLQNLMQNDSNENVPTVEGNDTEPPKSNQNYAFITPTEAG
                     FVPTNPNEIAGLPRPQPGMTFTPTGGSVSSINHQLNRQKEDTESLNNETSPNQQQ"
     gene            2762..2838
                     /locus_tag="PKV52_00565"
     tRNA            2762..2838
                     /locus_tag="PKV52_00565"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2796..2798,aa:Ala,seq:ggc)
     gene            2900..3448
                     /locus_tag="PKV52_00570"
     CDS             2900..3448
                     /locus_tag="PKV52_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98738.1"
                     /translation="MHINELLTHHGLDTTSWGKDGAKDVDELQREVCNGESQFVQNGE
                     VLQRLLRVVEASIYYTDKSGDTYRLAEEKQVMRSGVKRVRTELASVSMAEKMKVNEYA
                     LQALWRGIKEELGIGKEDYRVISGPQIYYKERTSNSYPGLRTRYEIARYVIELAPQAY
                     IADGYIEEQLDKSVHFSWSRDI"
     gene            3510..4796
                     /locus_tag="PKV52_00575"
     CDS             3510..4796
                     /locus_tag="PKV52_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD98739.1"
                     /translation="MKKIIKSILTSIFVRQAKAILKAHNPLIVVVVGSVGKTGTKLAI
                     ANVLKQKYRVCYQDGNYNVPLTLSFVLTGQQLPTLTNPFGWLKVWLKGQKYIYGRFPY
                     DVVVLELGTDAPGDIQNFKNIVNPDIAVVSAISEEHMEFFEEVEKVAEEELSVAQFST
                     TLVVGSDDCDKKYLQLFVPSSKKVIKYGFNAPAQYKVSAHRTSHHSFKVHISIPSKHA
                     VETDVNVAAKHSVKPLAAATAVADILGLSTKQIQKGLSTITPPPGRMRLLDGIQNTII
                     IDDSYNSSPLATEAALHALYDMKAPQRIAILGSMNELGAVSQIAHQKIGALCHPDKLD
                     LVVTLGEQANTYIAEAAEQQGCKVIRTLSPVKAGKVAAQNASSGAIILVKGSQNKVFA
                     EEAVKQLLANPSDIEHLVRQSDFWMAKKVAQFTDMQ"
     gene            4818..>8164
                     /locus_tag="PKV52_00580"
     CDS             4818..>8164
                     /locus_tag="PKV52_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPD98740.1"
                     /translation="MNKYTPREIEPKWQKKWETSQIYATTEDSTKPKRYVLEYFPYPS
                     GAAMHVGHVRNYTIGDAMARFYRMQGFNVMHPMGWDAFGLPAENYAIKNNISPERAIA
                     ENTAKFKQQLTQMGFSYDWSREINSSDPRYYKWTQWLFILLLNRGLAYQKDSLQWWCE
                     TDKTVLANEQVESGKCWRCGNAVTKKPLKQWFFKITDYADRLVDDLESLDWSESIKSM
                     QRNWIGKSQGAEITFNVQESINYVLLHGYTGSSKDCFFPWLSEKLTQSNASVFSPDLP
                     NTDNPNADVQAEYVLNNTEFSASTVLLGHSYGAVTALKVLEKLDTPIKRLVLVAGFVQ
                     PGFLDAERKFENTTNWEFNFDKIKRNVEEIVILRANNDSIVPADRADYIYNNIGGRII
                     DFDAHTNHVCGTVEPVVLEYVTTSIKVFTTRPDTLFGATFMVLAPEHPLVQKITTKDQ
                     RKAVDTYIAATQKKSDVERQETDREKTGVFTGAYAINPVNNVAIPIWIADYVLYGYGT
                     GAIMAVPAHDKRDYEFAKKFDLPIIDVVAQDFGTYLPDSKDVTGSVVVVYDQNTKKFL
                     GIKTGTNDGLWLPGGGSESGESFEATAMRELAEETGFHKYQKMVALGEPVYSYYFNDV
                     KDVNKRSYGQGFLFFVDAKDQGKTNLEKHETFQLGWYDYDQIIRGVKKVKGGVDHWLE
                     MLRRAKQVIDGHELEVFCGEGILVNSGAYNGTSSSEARESIVADLEKKGVGKEVTNYK
                     IRDWLISRQRYWGAPIPIIHCASCGAVPVPLEQLPVQLPPVKSYQPTGEGTSVLASVS
                     DWVNVACPTCGKPAKRETDTMDGFACSSWYFLRFADPHNSEAPFSAEKAQFWLPVDDY
                     IGGAEHAVMHLLYARMWTKVMYDAGLITFDEPFKALRNHGMILAPDGRKMSKSWGNVI
                     APDELIDQGYGADAIRIMELFIGPWNQEANWSVEGMGGCYRFLNRVWTLTQEYLAAST
                     NSKNESGELLTATHKAIKKVTIDMHNMGFNTAIAALMEYVNELYIMKAQHGFITKDWG
                     FALATLMQLLAPFAPHISEELWHQLGYDSSVHVAPWPEYQERYLVEHVMKIAVQVNGK
                     LRGEIEVEHSASKEAIEQEARMHQNVAT"
BASE COUNT         2553 a         1612 c         1830 g         2169 t
ORIGIN      
        1 tattgcctct acaattctgc tgtacgcact accaattgtc ggttcactac tcgaagcgac
       61 atttgtgtac actatcgggg ctttactcat ggcgctcagt ctttactggt tattggtgtg
      121 gtggccagcc ctgtatattg ccatgttgcc aggtacaacc cccatcggtg ctatgcgagc
      181 tgccgcaatg cttacaaaag ggcgtcgttt taaaatattt agtcgccaat ttgccatatt
      241 ggtcgtgctt ggtagcttat tttttgtcgt agtatttcca ctggctattg tctggcagcg
      301 tcttgtaccg cttagtgtat atgtaagtat cattacccta tatgggatcg gacacatagc
      361 tttatttaat ttataccgga gcttaattga tgaacagccc acagccacgc caaagaatta
      421 aacaactcgt tgagctacta aacacatatt cgtacgagta ccatgtgctt gataaaccaa
      481 ctgtagacga tgccatatac gacgcattgc atgctgaact agaaaactta gaatctacct
      541 atccagaact tatagaacca tcatcaccaa cccaacgaat tgtcagctca cccttgagtg
      601 gttttcaaaa ggtagagcac aaacatcgta tgttgggttt aagtgatatt tttagcaccc
      661 aagagctaga agattggcta agtaggatta caaaaattga tagtaaagta gccactaccc
      721 atttttgggc aagtatcaaa atggatggct tggcatgcgc tattatctac caaaatgggg
      781 tgcttaaaac agcaatcaca aggggtgacg ggtttgttgg cgaagatatc accaataacg
      841 ttaagacaat taaaagcgtt ccacttgcgc tgcctcataa tcacccattt tctgatggtc
      901 gcacagaact tcgcggcgaa attgttatgt ataaaactga ttttgctcat atcaacaaac
      961 agcttcaaga tgcaggagaa aaagtatatg ccaaccctcg taatctagca gcaggtacga
     1021 tgcgccaact agatgctaag gtagcagcca gccgtaagct gcattttata ccgtatgata
     1081 tattgcgcga tgacccagaa caaatctcta cctatcagtt tgtgtataac acgtttgcag
     1141 aactagggtt tatttcaagt agtcgtacag caaagctatt aaacagtgcc gatgatatac
     1201 tggcgtacta cgaatcatgg aaagataagc gtcatgaact gccgtacaat actgacgggt
     1261 tagtgataaa agtaaacgat agacagctgt atgcaacgct gggagtggtt ggtaaaaacc
     1321 cacgtggtgc agtggcgtta aaatacccag cagagactgc tacaaccatc ctaagagaca
     1381 tcttcattag tattggcaga acgggagctg caacgccagt ggctgtctta gaaccagtgg
     1441 tggttgctgg tagcaccgtc cagatggcaa ccatgcataa tgattcagaa atacaacgta
     1501 aagacgtgcg cattggtgat actgttgtta ttcaaaaagc gggtgacatt atcccagaag
     1561 ttgtacaacc tataaaagaa cttcgcaatg gtacagaaac tgaatttgtg atgcccgtgc
     1621 attgccccga atgtaataca aaactaacac gaccaaccaa cgaagttgta tggcgttgca
     1681 tcaatgtaaa ttgtccagca aggacctggc gacatattca acactacgct tctaaggcag
     1741 cgctagatat agagggtctc ggcgaaaaaa acgtgcaagc cttgcttgat gcacatctta
     1801 ttagcgatgc ggcagaccta tataccctac aaaaacaaac aatcagcaca ttagataggt
     1861 ttgccgatct cagtgctagc aacttagttg aagccattgc aaagaaaaaa aatccaccgc
     1921 ttgcgaagtt tatttttggt ctaggaattc ggcacgttgg tgcccagaca gctatcgaac
     1981 ttgttaatca ttacaaaaca cttgaagctt tatccgcagc atcattagat gagttgcaaa
     2041 ccatagacgg agttggagag gttgtggcgg aaagtatcat ttcttggttt tcggaccaag
     2101 aaaaccgtaa actactacgt aaatttgctt acaatggtgt agtcccacaa tcagtgacca
     2161 caaccactca gggttcatta cagggtatgt atattgcaat aactgggtca ctaagtagta
     2221 tgagcaggga agctgccgca gacaaaatac ggcaacgcgg cgggacgttt caatcgtccg
     2281 ttggtaaaaa taccacacac ctcgtagcag cgggtactat tgggtcgagt aagcttgcca
     2341 aagcagaaaa atttggtaca aaaataatag acgaacaagc cttcttacaa atggtacagt
     2401 aaaacaccct attgttgctg gttaggagaa gtttcgttgt ttagcgactc tgtatcttct
     2461 ttttgtctgt tgagctggtg attgatactt gaaacagatc caccagttgg tgtgaatgtc
     2521 atgccgggtt gtgggcgtgg taacccagct atttcattag ggttagtagg cacaaaacct
     2581 gcttcagttg gtgtaataaa tgcataattt tggttacttt tgggtggttc ggtatcgttg
     2641 ccttctaccg ttggcacatt ttcattagaa tcattttgca ttaaattttg cagatcttca
     2701 tcgttcatcg tattattatt gtagcaaata aaccgtaaac tagggtataa tcattgtgtt
     2761 tggggcattg gcgcagttgg ctagcgcgcc tgcatggcat gcaggaggtc aggagttcga
     2821 atctcctatg ctccaccaat acagcatttt gtagactata ttatgaaaca tctaacaccg
     2881 tgaaagacgt tttcattaca tgcatataaa cgaactactt acacatcatg gtctagacac
     2941 cacatcttgg ggtaaagatg gtgcaaaaga cgttgatgaa ctacagaggg aggtgtgtaa
     3001 tggtgaatcg cagttcgtac agaatggtga agtattacaa agactattac gagtagtcga
     3061 ggcatctata tactacactg ataaatctgg tgatacatat cgtctcgcag aagaaaagca
     3121 agtgatgcgc agtggagtaa aacgtgtacg cacagaactt gctagtgtat caatggctga
     3181 aaaaatgaaa gtcaatgaat atgccctcca ggcactctgg cgtggcataa aggaagaact
     3241 tggtattggt aaggaagatt atcgtgtaat aagcggacca cagatatatt ataaagagcg
     3301 tacctcaaat agttatccag gtctacgtac acgatatgaa attgcgcggt atgtgataga
     3361 acttgcaccg caagcatata ttgcggacgg gtatatagaa gaacagttgg ataagtcagt
     3421 acatttttca tggtctagag atatatagat gcgctattat tatgcatatc gttgactttg
     3481 tatcgtgtag tgtcgtgtaa taaagagata tgaaaaaaat aatcaagagt attttgacaa
     3541 gcatttttgt cagacaagcc aaggcgatat taaaagctca taacccactt attgttgtcg
     3601 tagtaggtag cgtaggtaaa accggtacca aattagctat tgccaatgtc cttaagcaaa
     3661 aatacagagt atgctaccaa gatggtaatt ataatgtacc ccttacactt tctttcgtgc
     3721 ttactggcca gcaattacca accctgacaa atccttttgg ctggctaaaa gtttggttaa
     3781 aggggcaaaa gtatatatat ggaaggttcc catacgatgt tgttgtacta gaacttggta
     3841 cagatgcacc tggtgatatt caaaatttta aaaatattgt aaacccagat attgccgtag
     3901 tgagcgccat aagcgaagag catatggagt ttttcgagga agtagaaaaa gtagcagaag
     3961 aagaattgtc tgttgcacag tttagcacta ccctcgtagt tggtagcgac gattgtgata
     4021 aaaaatatct acaattattt gttccaagta gcaaaaaagt cattaaatac ggttttaatg
     4081 caccagcaca gtataaagtt tctgcgcatc gcacgtctca ccacagcttt aaagtacata
     4141 ttagtattcc atctaaacat gctgtagaaa cagatgtaaa cgttgctgcg aaacatagtg
     4201 taaaaccact cgcagcggca actgcggttg cagatatact aggactaagt accaaacaaa
     4261 tacaaaaggg cctcagtacc ataacgccac caccaggccg aatgcgttta ttagatggta
     4321 tacaaaatac cataattatt gatgacagct ataattcgag cccgttagct actgaggctg
     4381 ccttgcacgc tttatacgat atgaaagccc cgcaacgtat tgcaatactg gggagtatga
     4441 acgaattggg cgcagtatcg caaatcgcgc atcaaaaaat aggtgcattg tgccacccag
     4501 acaagctaga ccttgttgtt acactcggcg aacaagcaaa cacctatatc gctgaggcag
     4561 cagaacaaca aggctgcaaa gtaattcgta cacttagccc agtaaaagcg ggcaaggtgg
     4621 cagcacaaaa tgccagtagc ggagcaatta ttttagtcaa aggatcgcaa aacaaggtgt
     4681 ttgcagaaga agctgtaaag cagctgctcg ccaacccgag tgatattgaa catctcgtac
     4741 gccaatcaga cttttggatg gcaaaaaaag ttgctcagtt tacggatatg caataacaga
     4801 ttataatgag caagataatg aataagtaca ctccaagaga gattgagcca aagtggcaaa
     4861 aaaaatggga aacatctcaa atatatgcca ccacagaaga tagcacaaaa ccaaagcgct
     4921 atgttttaga atattttcca tacccaagcg gtgctgcgat gcatgttggg catgtacgta
     4981 actatacaat tggcgatgca atggctcgct tttaccgtat gcaaggcttt aatgtcatgc
     5041 acccaatggg ctgggatgcg tttggtctgc ctgcagaaaa ttatgctatt aaaaataata
     5101 ttagtccaga acgggcaata gctgaaaaca ctgcaaaatt taaacaacag cttacccaaa
     5161 tgggttttag ctacgattgg tcacgagaaa tcaacagtag tgatcctcgc tactacaagt
     5221 ggacacaatg gctctttatt ttactactta acaggggttt ggcttaccaa aaagatagtt
     5281 tacagtggtg gtgcgaaacc gacaaaaccg tccttgcaaa tgaacaagta gaatcaggca
     5341 agtgttggcg ctgtggcaac gctgttacca agaaaccttt aaaacaatgg ttctttaaaa
     5401 taacagatta tgcagacaga cttgtagatg atttagaatc attggattgg tcagaatcta
     5461 ttaaatctat gcaacgtaac tggattggca aaagccaagg cgccgaaatt acttttaacg
     5521 tgcaagaatc cataaactac gtattactcc atggttacac tggatcttca aaagattgtt
     5581 tttttccttg gctatcagaa aaactcactc aatctaatgc tagtgtattt agcccagact
     5641 tgccaaacac agataaccca aatgccgatg ttcaggcaga atatgttttg aataatactg
     5701 aatttagcgc aagcaccgtg cttttagggc atagttacgg cgcagtaaca gcacttaagg
     5761 tattagagaa attagataca ccaatcaaga ggttggtatt ggtagcaggc tttgttcaac
     5821 ctgggttttt agatgcagag agaaaatttg aaaatactac aaattgggag tttaattttg
     5881 acaaaattaa acgaaacgta gaagaaatag ttatactgcg tgcaaataat gattctattg
     5941 ttccggccga cagagctgat tatatttata ataatattgg tggaagaatc attgactttg
     6001 atgcacacac caatcatgtg tgtggtactg tcgagccggt tgtgcttgag tatgtcacaa
     6061 catctataaa agtttttact actcggccag acacactgtt tggggctacg tttatggtgt
     6121 tggcgccaga acatccactt gtccaaaaaa tcactacaaa agatcagcgc aaagcagttg
     6181 atacatacat agctgcaacc caaaaaaaat ctgatgtcga acgtcaagaa acagaccgtg
     6241 aaaaaactgg tgtatttacc ggtgcctacg caataaatcc ggtaaacaat gtagcaatac
     6301 caatctggat tgctgattat gtgctatatg gctatggtac cggtgctatt atggccgttc
     6361 ctgcccacga caagcgcgac tacgaatttg ctaaaaagtt tgacctacca attattgacg
     6421 ttgttgcgca agactttggt acatatttgc cagacagtaa agacgtaaca gggtctgtgg
     6481 ttgtagtgta tgaccaaaat acaaagaagt tccttggtat taaaacaggc acgaatgatg
     6541 gtttatggct acctggtggc ggatcagaat ctggcgagtc atttgaagcc acagccatgc
     6601 gtgaactggc agaagaaact ggttttcata agtatcaaaa aatggtcgct ttgggcgaac
     6661 cagtctattc gtattacttt aatgatgtca aggatgtaaa caagcgctcg tacggccaag
     6721 ggtttttatt ttttgttgac gcaaaagacc aaggcaaaac aaatttagaa aaacacgaaa
     6781 cgtttcagct gggctggtac gactatgacc agatcattcg tggtgtaaaa aaagttaaag
     6841 gcggggtaga ccattggcta gaaatgctcc gtcgtgccaa acaagttatt gacggccacg
     6901 agcttgaagt tttttgtggt gaaggtattc ttgtcaattc tggtgcatat aatggtacaa
     6961 gcagttcaga agcgcgtgaa agtattgttg ccgatttaga aaaaaagggt gttggcaaag
     7021 aagtaaccaa ctataaaatt cgcgattggc tgattagtcg ccaacgatac tggggtgcac
     7081 ctattccaat tattcactgt gctagctgtg gggctgtacc agtgccatta gaacaactac
     7141 ccgtacaact accgcccgtt aagagttacc aaccaacagg cgagggcaca agcgtacttg
     7201 caagcgtgtc tgattgggtg aatgtagcgt gccctacttg tggcaagcca gccaaacgtg
     7261 aaacagatac catggatggt tttgcttgta gtagttggta tttcttgcgt tttgccgacc
     7321 ctcataattc tgaagcacca ttttctgccg aaaaggctca attttggtta ccagtagacg
     7381 attatattgg tggtgccgaa catgccgtta tgcacctatt gtatgctcgt atgtggacga
     7441 aagtgatgta cgacgccggg ctcataactt ttgacgaacc gtttaaggcg ctacgtaatc
     7501 atggtatgat tttggcgcca gatggccgca agatgagtaa aagctggggt aatgtaattg
     7561 caccagacga actcatagac caaggctacg gggccgacgc gattcgtatc atggaactat
     7621 ttattggccc atggaaccaa gaagcaaact ggtctgttga aggtatgggt ggctgttatc
     7681 gctttttgaa tcgggtgtgg acactcactc aagaatacct cgccgctagc accaattcaa
     7741 agaacgaatc gggcgagttg cttacagcta cacacaaggc cataaaaaaa gtcaccatag
     7801 acatgcataa catgggcttt aacaccgcaa ttgccgcctt aatggaatac gttaatgagt
     7861 tatacattat gaaagctcag catggtttta taaccaaaga ttggggtttt gcgctggcta
     7921 cacttatgca attgctggca ccgtttgcac cacatatcag cgaagaatta tggcaccaat
     7981 tgggctatga ttcatcagta cacgttgctc cgtggccaga gtaccaggag aggtatttag
     8041 tagaacatgt tatgaaaata gcggtgcaag tcaacggcaa gttaaggggc gaaatagaag
     8101 ttgaacacag tgctagtaaa gaagccatcg agcaagaagc ccgtatgcat caaaacgttg
     8161 ctac
//
LOCUS       DAOPLS010000012         7732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_10074_length_7732_cov_4.176501, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000012 DAOPLS010000000
VERSION     DAOPLS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10074_length_7732_cov_4.176501"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKV52_00585"
     CDS             <1..743
                     /locus_tag="PKV52_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98741.1"
                     /translation="VTQGYGADTLLQRGMIVGLQKDDPRKVEPINSDDYDRLHGVVIG
                     ANESAVLLGRDDEKVYVASGGRFPVLVSSQNGNIAVGDYVAASSVKGVGMRAGDIEPV
                     ILGKAIEAFDSGNQDEIKSAVTVKDNNGNEQRLAVGIVTVDVSIGKNPMLKSNNDLPT
                     ALRRASELVAGKPVSPIRVYMSLAILVVATAISGSLIYSAVRSSITAIGRNPLSKKAI
                     MRGLFQVVIIGLIVFISGIFGVYLLLKL"
     gene            774..1394
                     /locus_tag="PKV52_00590"
     CDS             774..1394
                     /locus_tag="PKV52_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98742.1"
                     /translation="MTTTTIALIIAALVVGGAVFIWFILRGEKTEQSGRDIDTQLANT
                     AKTGVDKIFDEEFREELRNRGRLHFEKIIGENAMFLQQDLRLTTTQLNEYMKTEIKRT
                     LQSEFSKYEESITTAKDLALESIEKTQAVIEQQRLVLEKQMTDEAAAEKARMLSSFEK
                     NMADIVNHYILEAIGNEIDLTVQLDYIFGYLEENKQAIMEDIKSGS"
     gene            1384..1857
                     /locus_tag="PKV52_00595"
     CDS             1384..1857
                     /locus_tag="PKV52_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98743.1"
                     /translation="MDLKLPTTIASRKDIIRLHREIRSFIDVSTQSIMRHDNPVKYPA
                     ISDQLRALAVLNQIDMRDPKSCERLLQKIDTIKEHAPSVHISFPTEPSADILERLIVW
                     FRKEIDPFIVIQVGLQPTIAAGVVVRTPNHQFDFSLRRHLYANRHKLGEALQRAY"
     gene            1847..3274
                     /locus_tag="PKV52_00600"
     CDS             1847..3274
                     /locus_tag="PKV52_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HPD98744.1"
                     /translation="MPTRHFDFLIDKGHPIGEVIGVDRYLVRLRGMHPVNQHSLVLFE
                     DGSKGFVQYIYDDHVEVLHLGDSTLTVGMTAVMQHNELVCKVGKDFIGRVINVMGEPL
                     DGKGPIAADATRPVFNSAPPIYQRKQLSDQLETGIISIDSLFPIVKGQRMALLGDSKS
                     GKSTLVTQLALHQKSTNIPTVYVLIAKRRADVDLLLGRLQEEDALKNTIVIVSTLFES
                     LVLSYLAPYIGCSMAEYFWQDLDQDAIIIYDDLTSHAMAHREISLLAGVSPGRDSYPG
                     DMFYAHSSLLERAGRLARNTKTLTSFPMVHAAGGDITAYLPTNIMSITDGQWILDMDI
                     FRDGIRPALNTGLSVTRVGGVGHNKRQHEIAARVMKTLGSYRQALEFSRFGSELAIEA
                     KRDLNTGKIIFELMTQTPTETFSVMAQQLMFEIVLDLEDGELLDLQTLKSLSGELAAK
                     VKEDDSNYDETKAQLKKKSLLEMKK"
     gene            3271..4125
                     /locus_tag="PKV52_00605"
     CDS             3271..4125
                     /locus_tag="PKV52_00605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HPD98745.1"
                     /translation="MRRPIEIQKDVIGMTTLSELTSVFEGIASMRIAQIKNQVVQSQN
                     YFDRLWQMYSQMSVGSVFSFGRHTDKTKIIEKELYIVITAEGGFSGDIDQKLITWMLK
                     NYHKDKNDIVVIGHHGAIQLAQRGVSFKKYYKLPAKDENINVTPIIREVQKYASTIVY
                     YQTYVSLAVQDVKRISLSSAVQDQGKDVKKSEEVINELTYIFEPSTFAVVDHLERSMM
                     TIALSQVILESKLAQYASRFQAMTASHKRASDEVSDLKLQYNRSKRAIKDERLKEIIN
                     GMRKAHHV"
     gene            4122..5426
                     /locus_tag="PKV52_00610"
     CDS             4122..5426
                     /locus_tag="PKV52_00610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPD98746.1"
                     /translation="MTTGNVLIVKDLVVRVEFTSDMPEIGEVLVVQNEEKSLLLVDAL
                     ASDTVATCINLRSSKHIQKSMAVERTGKGIEIPVGEASIGRIFDALGEPLDGMDPVPE
                     GTPRRNILKLGAKSTTFEVAKPEILETGIKVLDFFTPLVKGRKIGVIGGAGVGKTVLT
                     MELIHNIARTGKGLSFFAGVGERVREGHELYETLQEYDLLKNTCMLFGQMNENPAQRS
                     LIAISATAMAEYFRDEEKKDILFFADNMYRFVQAKNELATIMDQIPNEGGYQPTIFSD
                     VKILQDRLSSNENGSITSLQTIYVPADDLSDPAVQVISHELDSTIVLSRKVAEQGIRP
                     AVDLNLTNSSLLSPDVVGDRHYMLSVRVQSILQKYEQLKGIIAIIGESELSPADRAEY
                     AKAKKLIQYFTQHMFVTEKLIGIKGEYFTRDETLKGIEEILV"
     gene            5444..5758
                     /locus_tag="PKV52_00615"
     CDS             5444..5758
                     /locus_tag="PKV52_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98747.1"
                     /translation="MAKKQSTPHAATSDANDAVPKDKFHLKVYAPFKVYYEGVVASIS
                     AENGTGPFDILANHHNFLTLLNPCELTIRTSTEDKEPEKIKITRGIMQVKANDVVVFL
                     DV"
     gene            complement(5792..6811)
                     /locus_tag="PKV52_00620"
     CDS             complement(5792..6811)
                     /locus_tag="PKV52_00620"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPD98748.1"
                     /translation="MEDNKPNPQDTSETVEPTSTEIEQGSTSTEASESLDATVNKNTE
                     TTDDTQRFIPKKGIGQRVQKTVSGINIYMLLFILVLFIVAFIGFASYSSNKKAENQSV
                     INGQELSQQDLENLASSDQQVGDAQQTLTIASNAVFNGRVLVRNDLDVAGTIRVGGAL
                     SLPGITVSGTSAFENVQVANNLSIAGNSAVQGTLTVQQNLSVGGSASFAGQISAPSLS
                     VERLILNQDLQINRHIDAGGPTPVASRGSAVGGAGTVSVSGTDTAGTVSINFGPGSAA
                     GIIASINFANNFSQTPHVVITPVGSSCANLNYYVNRTTGGFSIGTTNAGGPGTSCAFD
                     YVAID"
     gene            6933..7682
                     /locus_tag="PKV52_00625"
     CDS             6933..7682
                     /locus_tag="PKV52_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98749.1"
                     /translation="MRKQTLVRTSIITIALSLVALPGFLARAYAAPTYVSPNYGVDEV
                     FFGAGGVNDANSANYNARASLGDTGVGNSASSLYQLYGGFTTTEVPFLEFVVNAVNVD
                     LGTLTTASASTGTATFTVKAYLASGYVVSTISDPPTNASYTMANMPATAASSPGTEQF
                     GINLRSNTSPNTAGSDPVQLPDASFSFGQVSTGYDTPNQYKYVKNDTIAYSDKSSGIT
                     QYTMTYLMNIKSTTPGGVYVMNHNLVATGTF"
BASE COUNT         2364 a         1561 c         1707 g         2100 t
ORIGIN      
        1 cggtaacgca aggctacggg gcagatacgc ttttgcaacg tggcatgatt gtaggccttc
       61 aaaaagatga tccacgcaaa gtagagccta ttaattctga cgattacgac agactacacg
      121 gtgtagttat tggcgccaat gaatcagctg tattgcttgg ccgcgacgat gaaaaagtat
      181 atgttgcgag cggcgggcga ttcccagtgc tcgttagtag ccaaaacggt aatattgcgg
      241 ttggagatta cgtggcagca tcatcggtta agggtgtagg tatgcgcgca ggtgatatag
      301 aacccgttat tcttggcaaa gctatcgaag catttgattc aggtaaccaa gacgaaataa
      361 aatcggcagt cactgttaag gataataatg gcaacgaaca acgtttagca gtaggtattg
      421 taacggtaga cgttagtatt ggcaaaaacc cgatgctaaa aagtaataac gatttaccta
      481 cagcactacg gcgagcaagt gaactcgtgg caggcaaacc agttagccct atacgcgtgt
      541 acatgagcct tgctatttta gtggtagcga ctgctatatc tggtagtctc atatatagcg
      601 ccgtccgtag tagtatcact gccatcggtc gtaatccgct gagtaaaaaa gccatcatgc
      661 gtggtttgtt ccaagtagtt ataattggtt tgattgtttt tatatcgggt atttttggcg
      721 tatacttatt actaaagttg taagaataaa cagtatcagg gggtggcaat tttatgacaa
      781 caacaactat cgcacttatc atagcagcgc ttgttgttgg tggcgccgta tttatatggt
      841 ttatattacg tggtgaaaaa actgaacaat caggccgtga catagacacg cagttggcca
      901 ataccgccaa aactggtgta gataaaatat ttgatgaaga gtttagagaa gaacttcgta
      961 acagaggccg tttacacttc gaaaaaatta ttggtgaaaa cgccatgttt ttacaacaag
     1021 atttacgtct gactactacc cagttaaatg aatacatgaa gacagaaatt aagcgtactc
     1081 tacagtcaga gttctctaag tacgaagaat caatcacaac tgcgaaagat ttggctctcg
     1141 aaagtataga aaaaacgcaa gcagtgatag aacaacaacg tcttgtgcta gaaaaacaaa
     1201 tgacagatga agctgctgcc gaaaaagcac gtatgcttag tagttttgaa aaaaacatgg
     1261 cagatatagt aaatcactac atcctagagg caattggtaa tgaaattgac ctaactgtac
     1321 aacttgatta catctttggg tatttagaag aaaacaaaca ggccattatg gaggatatta
     1381 agagtggatc ttaaactgcc gactaccatt gccagcagaa aagacatcat ccgattacat
     1441 agagaaatac ggagctttat tgacgtatct acgcaaagca ttatgcgcca tgataatccg
     1501 gttaaatatc ctgctatttc agatcagtta cgagccttgg ccgtcctcaa ccagattgac
     1561 atgcgcgacc caaaaagctg cgaaagactc ctgcaaaaaa tagacacaat taaagaacac
     1621 gctccaagtg tacacattag ttttccaacc gaaccatcag ccgatattct tgagcgactg
     1681 attgtttggt ttcgtaaaga aatagatccg ttcatagtta ttcaagttgg actgcaacca
     1741 acaattgctg ctggggtagt agttcgcaca ccgaaccatc agtttgattt tagcttacgg
     1801 cgtcatttat atgccaaccg gcacaaatta ggggaggcgt tgcagcgtgc ctactagaca
     1861 ttttgatttt ttaatagata aagggcatcc aattggagag gtaattggtg ttgatagata
     1921 tcttgtgcgc ttacggggta tgcacccggt aaaccagcac agtttagtgc tgtttgaaga
     1981 tgggtcaaaa ggctttgtac agtacatata tgatgatcat gttgaagtac tgcatttagg
     2041 cgatagtacg ctgaccgttg gtatgacagc ggtgatgcag cacaatgagc tggtctgtaa
     2101 ggttggcaag gattttatag gccgggtaat taatgttatg ggtgaaccac ttgatggtaa
     2161 aggcccaata gcagcagatg cgaccagacc ggtttttaac agtgctcctc ccatttatca
     2221 gcgaaaacag ctttcagacc aattagaaac aggcattatt tctatagata gtttgttccc
     2281 tattgttaaa ggccagcgta tggcactcct tggcgatagt aaatctggta agagtacgct
     2341 cgtcactcaa ttagctttgc atcaaaagtc aacaaacata cccacggtgt acgtgcttat
     2401 tgccaaacgt cgtgctgatg ttgatttgtt gcttggtcgc ctccaagaag aagacgcttt
     2461 aaaaaataca attgttattg tgtctacatt gtttgaatct ttggtcctta gctacttagc
     2521 gccatatata ggttgttcta tggcggaata tttttggcaa gacttagacc aagacgccat
     2581 tatcatatac gacgatctca ctagccatgc tatggcacac cgtgaaattt cattactagc
     2641 gggagtcagc cctggtcgtg attcttaccc cggtgatatg ttttatgcac acagtagttt
     2701 actagaaaga gctggccgct tagcacgcaa cacaaaaaca ctgacgtcgt ttccgatggt
     2761 acatgctgct gggggtgata tcacagcgta tttaccgacc aatatcatgt ctattaccga
     2821 tggacaatgg atcctagaca tggatatatt ccgtgacggt attaggcccg ccttaaacac
     2881 tgggttaagc gtaacgcgtg tcggtggtgt tggccataat aaacgccaac acgaaattgc
     2941 tgctcgggtt atgaaaacac tcgggtcata ccgccaagcg ctcgaattct cacgatttgg
     3001 ctctgaactt gccatcgagg caaagcgtga tttaaataca ggtaaaatta tattcgaact
     3061 tatgacacaa accccaaccg aaacttttag tgttatggcc caacagttaa tgtttgaaat
     3121 tgtgctagat ctagaagatg gtgaactact tgatttacaa acactcaaat cgttgagtgg
     3181 tgaactcgcc gcaaaagtaa aagaagatga cagcaactac gatgaaacaa aagcgcagtt
     3241 aaaaaagaag agcttactgg agatgaagaa gtgagacggc caatagaaat ccaaaaagat
     3301 gtcatcggca tgacgacctt atctgagctt accagcgttt ttgagggtat cgccagtatg
     3361 cgtatcgccc agattaaaaa tcaggtggtg cagagccaaa actattttga tcggttatgg
     3421 caaatgtata gtcaaatgag tgtgggttct gtatttagtt ttgggcgaca cacagataag
     3481 actaagatta ttgaaaaaga actgtacata gttatcactg cagagggtgg ttttagtggt
     3541 gacattgacc aaaaacttat cacctggatg ctgaaaaatt accacaagga taaaaacgat
     3601 attgtagtta ttggtcacca cggcgctatc cagcttgccc aacgcggtgt gagctttaag
     3661 aaatattata agctacccgc caaagacgaa aatataaatg taacacctat tattcgcgaa
     3721 gtgcaaaaat acgcttctac gattgtgtat tatcaaacct atgtatcgct tgccgtccaa
     3781 gatgtaaaac gtatttcact aagttctgca gtgcaagatc aaggaaaaga tgttaaaaag
     3841 agcgaagaag ttattaatga acttacctat atttttgaac cgagcacctt tgcagtggtt
     3901 gatcacctag aacggtctat gatgacgata gctttaagcc aagttatttt agaatctaaa
     3961 ctcgcccaat atgccagtcg tttccaagct atgacagcat cgcataaacg cgcgagtgac
     4021 gaagtgagtg atctaaagtt acaatataac cgatcgaaac gtgccattaa agatgaacga
     4081 ttaaaagaga ttatcaatgg catgagaaag gcgcaccacg tatgacaaca ggcaatgtac
     4141 taatagtaaa agatcttgtt gttcgggttg aatttaccag tgatatgccg gaaattggcg
     4201 aagtgcttgt tgtgcagaac gaagaaaaga gcctcttgtt agtagatgcg ctcgccagtg
     4261 acactgtagc gacgtgtata aatctacggt ctagcaagca tatccaaaag tcaatggcgg
     4321 tagagagaac aggcaagggg atagaaattc ctgtcggaga ggcgtctata ggacgtatat
     4381 ttgatgcact gggtgaacca cttgatggta tggacccagt accagaaggt acccctcgta
     4441 ggaatatctt aaaattaggc gctaagagca cgacctttga ggtagctaaa ccagaaatcc
     4501 tcgaaactgg cataaaagtg ctcgatttct ttaccccact ggtaaaaggt cgtaaaattg
     4561 gtgtgatagg cggcgcaggt gttggaaaaa ctgttcttac aatggaactt attcacaata
     4621 ttgcccgtac cggtaagggt ctctcgtttt ttgcaggcgt tggtgagcgt gttcgtgaag
     4681 gacacgaact atacgaaacg ttgcaagaat atgacctact gaagaatacc tgtatgttat
     4741 ttgggcaaat gaatgaaaac cctgcccaac gtagtcttat tgctatatca gctacagcaa
     4801 tggctgaata tttccgtgac gaagaaaaga aagacatttt gttctttgca gataacatgt
     4861 accgttttgt gcaggctaaa aatgaactgg cgactatcat ggaccagatt ccaaacgaag
     4921 gtggctacca gccaactatc ttttcggatg taaagatatt acaagatcgt ctgagttcta
     4981 acgaaaatgg ctcaatcacg agcctgcaaa ccatttacgt gccagccgac gacctttctg
     5041 acccggctgt acaggtgata tcacatgagc ttgactcaac aattgtactt agccgtaaag
     5101 ttgccgaaca gggaatccgc ccagctgtcg atttaaacct tactaattcg agcttgttat
     5161 cgccagatgt cgtgggcgat cgccactata tgttgagcgt tcgagtacag tctatattac
     5221 aaaaatacga acagctcaaa ggtattattg cgattattgg tgaaagtgaa ttatcaccag
     5281 cggacagagc agaatatgcc aaagccaaaa aactgataca gtactttacg cagcatatgt
     5341 ttgtgacaga aaaattaatt ggtataaaag gcgaatactt tacacgtgac gaaactctta
     5401 agggtataga agaaatcctc gtgtaggtta gtgaaaaacc attatggcaa aaaagcaaag
     5461 caccccccat gcagctacat cggacgcaaa tgatgctgtg ccaaaagaca aatttcacct
     5521 aaaagtctat gcaccattca aggtgtatta tgaaggagta gtggcaagta tttcagcaga
     5581 aaacggcaca ggcccgtttg atatcttggc aaaccatcat aactttttaa cactacttaa
     5641 cccgtgtgag ctcactattc gtacatctac agaagacaaa gagccagaga agattaaaat
     5701 aacccgtgga atcatgcaag taaaagcaaa cgacgtggtt gttttcttag atgtctaggt
     5761 atcgcttatt ttgtaaaact acgttgttgt tttaatcaat ggcaacataa tcaaaggcgc
     5821 agctcgtacc aggaccccca gcatttgtgg tcccgatact aaatccacca gtagtacggt
     5881 ttacgtaata gtttaaatta gcacaacttg accctaccgg tgtaataaca acgtggggtg
     5941 tctgtgaaaa attattagca aagttaatgc ttgcaataat accagcagcc gagcctggtc
     6001 caaaattgat acttacggta cctgctgtat cggtaccact tacgcttact gtacctgctc
     6061 caccaacagc gcttccgcgt gatgcgacag gtgtaggacc acctgcgtct atgtgccggt
     6121 taatttgtaa atcttggttt aaaatcaatc gttccacact aagagacgga gcggatattt
     6181 ggccggcaaa acttgcactg ccgccaacac tgaggttctg ttgaacagtg agagtacctt
     6241 gtacggcaga attaccagcg attgataagt tattggcaac ttgcacgttt tcgaacgcac
     6301 ttgttccaga tacggtaata cccggcaagc tcagtgcacc accaacccgt atagtacctg
     6361 ctacatcaag atcgttacgt actaacactc ggccattaaa cacggcatta gaagcaattg
     6421 tcagggtctg ttgagcatcc cctacctgtt ggtcactaga agccagattt tctaggtctt
     6481 gctggctaag ttcttgaccg ttaataacag actgattttc ggccttttta ttactggaat
     6541 agcttgcaaa tccaataaac gcaacaataa aaagcactaa aataaacaaa agcatatata
     6601 tattgatgcc actgactgtt ttttgaacac gctggccaat gcctttttta gggataaatc
     6661 gttgggtgtc atcagttgtt tcggtatttt tattaacagt cgcgtctagc gattcacttg
     6721 cttctgtaga agtactgcct tgttctattt ctgttgacgt aggctctacg gtttcacttg
     6781 tgtcttgtgg gtttggttta ttgtcttcca tttttttgtg atttaaccac ctttaattgc
     6841 ctactattgt aacaaatact atatataagc gctagcaatt tgcacaagat tcataagcag
     6901 tatacaatat gtagtataag caaaagatac gtatgagaaa acaaacacta gtccgcacta
     6961 gtatcattac catcgcctta agtcttgtag cgctcccggg ctttttagct cgtgcttacg
     7021 ctgcgcctac ctacgtatcg cctaactatg gtgtagatga agtctttttt ggcgcaggtg
     7081 gcgtcaatga tgcgaacagt gcaaattata atgctcgggc gtctcttggc gacactggtg
     7141 taggtaattc agcgagtagc ctataccaac tttacggagg ctttaccact acagaagtgc
     7201 ctttcttaga atttgttgtt aatgctgtca atgtagatct tggtaccctg actaccgcat
     7261 ctgcgagtac aggaacagcc acctttactg ttaaggccta tttggccagt ggctacgtgg
     7321 taagtactat ttcagatccg cccacaaatg cgtcatatac catggccaac atgcctgcta
     7381 ccgcggcgtc tagtccaggt acagagcagt ttggtataaa cttacggagt aacactagcc
     7441 caaacacagc tggttcagat ccagtacagc tgcctgacgc ctcttttagt tttggccaag
     7501 tatcaactgg gtacgacaca ccaaatcagt ataagtatgt aaaaaacgat accattgcct
     7561 atagcgataa aagtagtggc attacccaat atacaatgac gtatcttatg aatataaagt
     7621 ccaccacacc aggtggtgta tacgttatga accataactt ggttgcaacc ggtacgtttt
     7681 agactgtgga taactttact gtaaagtgta ttgaattata cacgcttatg gt
//
LOCUS       DAOPLS010000013         7717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_10098_length_7717_cov_3.673714, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000013 DAOPLS010000000
VERSION     DAOPLS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10098_length_7717_cov_3.673714"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKV52_00630"
     CDS             <1..634
                     /locus_tag="PKV52_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPD98750.1"
                     /translation="VDSRQVKTGFAEKFRAVLRLDPDVVMVGEIRDNDTAKTALQSAL
                     TGHLVLSTYHAGSAAASLTRMLDAISENPLFASAIRVIMAQRLIRRLDDTTKQPYTPD
                     ASTIAWLKKIIDTLPTRIEKPNLDNVQLYKAVPSATSPFGYSGQFAVRELLLMTPTVE
                     AELKKPIREITERSIQQVAIQDGMTTMLQEGVLRALAGETTIEEISRVLV"
     gene            complement(631..1764)
                     /locus_tag="PKV52_00635"
     CDS             complement(631..1764)
                     /locus_tag="PKV52_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPD98751.1"
                     /translation="MNIYEEALRAHKKARGKLAIVSKVPLKSRHDLSIYYSPGVAAVS
                     ERLAKHPSETPLYTNTHNSVAVVSDGTSVLGLGNIGPEGALPVMEGKAILFKHYAGID
                     AIPIVLNTQDPDAIIETVVAIAPSFGGINLEDIAAPQCFYIEDELKKRLSIPIMHDDQ
                     HGTAVVVLAGLINATRYVKKDLSACKVVLVGAGAAGIAIAKLLTQYAQPTIVAVDSKG
                     VITTSRSDLTPEKQMLAQLSKNDGSTSLEQAIKGSDIFIGVSRSGLLTEAMVRTMAEN
                     PIIFALANPEPEILPTVAKAAGAAVVATGRSDYPNQVNNVLAFPGLFRGALDTHSAIT
                     DAHKMAAAEALAALVTHPTADAIIPSPFDRHVADNVAAVFKKR"
     gene            1872..2390
                     /gene="rplS"
                     /locus_tag="PKV52_00640"
     CDS             1872..2390
                     /gene="rplS"
                     /locus_tag="PKV52_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPD98752.1"
                     /translation="MQSVIQAIESKYKKPQVVDVRTGDSVKVHQKIKEGAKERVQVFE
                     GLVIRTDRKNSLTSSITVRRLASGIGVEKSFIIHSPNVLKVEVTKRSKVRRNYLSYMR
                     VRTGKSARLTSVDFDKRAVNTIHDAQAEAAEAKIHAEAEADHEAAAQAEAAEATAETK
                     KFEEVAKDRAND"
     gene            2404..2949
                     /locus_tag="PKV52_00645"
     CDS             2404..2949
                     /locus_tag="PKV52_00645"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPD98753.1"
                     /translation="MIGIDEVGRGSWAGPLVVCAARLNKPINGLKDSKLLSKKRREEL
                     FVHIEKNCDIGVGWVSADEIDDIGLSAALYYAAGCALAELSPTSEEVVIDGSIQLLPA
                     YEASTVRIKADNTVPAVSAASIYAKVIRDNWMCSQDSIYDAYDFWHNVGYGTLRHRKA
                     IEQYGITPLHRTSFSLPSRIV"
     gene            2966..3304
                     /locus_tag="PKV52_00650"
     CDS             2966..3304
                     /locus_tag="PKV52_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPD98754.1"
                     /translation="MNTTQVGRQAEQAAVQWLLQNGYQIIDQNWQTRWCEIDIITKKA
                     NVIFFVEVRYRRNDAWGDGIDSITPRKLQQMQFAAELWLTQAKWSHDASLCAMAVAGQ
                     PPVVSAFIEL"
     gene            complement(3291..5804)
                     /locus_tag="PKV52_00655"
     CDS             complement(3291..5804)
                     /locus_tag="PKV52_00655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPD98755.1"
                     /translation="MTDVARLYREFTPTHYDVHLTLDATLLRFSGTVTVFGDRKKSSP
                     HIRLHVKDLQIIGVTVDSTACDFSINEDILDIHIPNIKKTSISATVHFSGTITKAMHG
                     IYPAYTNDGSVILGTQFESHHAREAFPCIDEPEAKATFALQITTHEPIVLSNMPQQTS
                     HQNTDDSTTFIFETTPVMSSYLVAFVCGNLQKVSTQTENGTEITVWSSKDHDIESLVF
                     PLEVAVKTTEFFNEYFGVAYPLPKCDHVALPDFSSGAMENWGLITYREIALIADPDTI
                     STSAKEYIATVIAHEISHQWFGNLVTMQWWDDLWLNESFATLMEYLVIDAIYPEWNVM
                     LSFAAHDALSAFRRDVLPGVQPVATPVHHPDAISTLFDPSIVYAKGARLLYMAYHVVG
                     DRNFKKGLRNYFKHHAYQNTVGADLWNALGAASGIDVASIMHGWITQSGFPYIKITPV
                     DDTTLSVEQHQLTTTMHTSEKLWSIPLWSSDNSALDILDTKRKLVHVQKPHTRFNIYG
                     GHYVPYYTSDATRLAIYDDVKHHRISPDARLLLLHDPLLLARCGVGSLTDALTSLSYY
                     VNETEQSVWSVISLVIGDTRLMIEGNETAESSLKKLTYRLIEKQYTRLGLVSKVTDTN
                     NDKKLRSIIAGLAIYSEEPNVIKTATKIFNDCDDFEALPVDTRSAILTAVIKSGDKAM
                     FDSFLTAYPATKNGDIQLDIAGALCATKDTTRIKKLISRLKDQQFVRLQDLDRFLVYL
                     LRNQASRASAWQWLVTNWHWITEVFAQDKSYDNYPRYSAAVFSTQEWLNTYAEHFTPL
                     ADIPSLARNIDLGTKDITAKIEWRQRDQKAVESWLSTAL"
     gene            5924..7087
                     /gene="nusA"
                     /locus_tag="PKV52_00660"
     CDS             5924..7087
                     /gene="nusA"
                     /locus_tag="PKV52_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPD98756.1"
                     /translation="MDIETKQLVMAVRTIAEEKNLPEDVVQEAIEQALAAAWRRDFGD
                     KDQEVRSVININDGSVTVFITKTVVDNVTNEHLEISLKDAQAIRKDVALDETVEIEEK
                     VENFGRVAAQTAKQVILQRLREAEREVVLAEFEDKIHTVLNAIVTRIEGRMVRIEINR
                     AQGILPPSEQIQGERYHPGQRLKVYLKDVERGFKGAQLVVSRGCPEFIEHLFRNEVPE
                     MEGGAVEIKGIAREAGVRTKIAVASNLPGVDPVGTFVGGHGTRVQAVMSEIGEQEKID
                     IVVFASDTKEYIINALSPTKVVSVELNENAKKAKVTVEEDQLSIAIGRSGQNVRLASR
                     LTGYDIDVVGDKPAAAEVPEKKDTVVGASNAPKLKKKSDLESSLLEAIEEHGE"
     gene            7215..>7717
                     /locus_tag="PKV52_00665"
     CDS             7215..>7717
                     /locus_tag="PKV52_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPD98757.1"
                     /translation="MNESQDMQEFLNHLTDNALMSLKHADHVARTQGSAYVGTEHILL
                     GVLAQDNSVASKILTTAGVSYERAQLALNLTPKNLVINLGAKGLSETAKLALKVSWDY
                     AQDYGQDMCGTEHIVFSILQQKNSRATVLLRDMNINTDKLLNSFDDFLQKQQFDSDQS
                     RKRTATKS"
BASE COUNT         2300 a         1540 c         1743 g         2134 t
ORIGIN      
        1 tgttgattct cggcaggtta aaacaggttt tgctgaaaag ttccgggcgg tgctacggct
       61 cgacccagat gttgttatgg ttggagaaat tcgtgataat gacacagcaa aaaccgcttt
      121 acaatcggcc ttaacgggtc atttagtgct ttctacctac catgctggtt cggctgcagc
      181 ctcgcttact cgcatgctag acgctatttc cgaaaaccca ctgttcgcat ctgccatacg
      241 agtaattatg gcgcaacgac tcatacgtcg cttagacgac acaacaaaac agccatatac
      301 acctgatgcg tctacgattg catggttgaa aaaaattatt gacacattac caactagaat
      361 agaaaaacca aatctagaca atgtgcaact atataaagca gtaccaagcg ctacatctcc
      421 ttttgggtat agcggccaat ttgcagtgag agagttgttg ctcatgaccc caacagtcga
      481 agctgaacta aaaaagccta ttagagaaat tacagagcgt agtattcagc aagtagctat
      541 ccaagatggt atgaccacca tgctacaaga gggcgtgttg cgagcgcttg caggcgaaac
      601 tacaatagaa gaaatcagtc gcgtacttgt ctagcgcttt ttaaatactg ccgctacatt
      661 atcggcgaca tggcgatcaa atgggctagg aataatggca tctgcagtag ggtgagtcac
      721 cagtgcagct aatgcttcgg ccgccgccat tttatgtgca tctgtaatag cactatgtgt
      781 gtctaacgct cccctgaaca agccaggaaa tgccaagaca ttattgactt gatttgggta
      841 gtcgcttcgc ccagttgcca cgacagccgc tccagcggct tttgccacag taggtaaaat
      901 ttctggctct gggttggcaa gtgcaaatat aataggattc tctgccatag tacgcaccat
      961 agcttcagtt aacaaaccac tgcgtgaaac gccaataaat atatcactgc ctttaatagc
     1021 ttgttctaaa ctagtagatc catcattttt agataattgt gctagcatct gtttttctgg
     1081 agttaagtct gatctagaag tggttataac tcctttgcta tctaccgcta caattgttgg
     1141 ttgagcgtat tgtgtgagca gtttagcaat agcgattcca gccgctccag caccaacaag
     1201 aacaacctta caagcactaa ggtctttctt aacatagcgt gtcgcattaa tgagcccagc
     1261 taacacaact acagcagtac cgtgttgatc atcgtgcata atgggtatcg aaagacgctt
     1321 tttaagttca tcttctatat aaaaacactg tggagcagca atatcttcta aatttatacc
     1381 accaaagctt ggcgctatgg caacgactgt ttctataatt gcgtctgggt cttgcgtgtt
     1441 tagcacgata ggtatggcat cgatacctgc ataatgctta aacaggatag cttttccttc
     1501 cataactggc agggcaccct cggggcctat attgccaagt cctagcacac ttgtgccatc
     1561 tgacacgacc gcgaccgagt tatgagtatt ggtataaagt ggggtttctg aaggatgctt
     1621 cgctaacctt tctgaaacag cagcgacacc tgggctgtaa taaatgctta aatcgtgccg
     1681 tgactttaat ggtactttag atacaatggc gagtttgcca cgagcttttt tatgcgcacg
     1741 gagtgcttct tcgtatatgt tcatgcttgt atctattgta cacatctaat cttgtatttt
     1801 ttagcctttt atattgtggg tttatgtgct atagtaaacc actgattgta ataataattt
     1861 tgggataaat catgcaaagt gtcatacaag ctattgagtc aaagtataaa aaaccccaag
     1921 ttgttgacgt aagaaccggc gacagtgtta aggtacatca aaaaattaaa gaaggcgcaa
     1981 aagagcgtgt tcaggtattt gaaggtcttg tcataagaac tgatcgtaag aatagtctca
     2041 ccagtagtat tactgtaaga aggcttgcga gcggtattgg tgtagaaaaa tcattcatca
     2101 tacacagccc aaacgtttta aaagtagagg tcaccaaacg aagtaaagta cgtcgtaact
     2161 atttaagcta catgcgtgtt cgtactggta aatctgcaag actgacaagt gtagattttg
     2221 ataaacgagc ggtcaatacc atacatgatg cacaggcaga agctgccgag gcaaaaatac
     2281 atgccgaagc agaagccgat cacgaagcag ctgcacaagc cgaagccgcc gaagcaacag
     2341 cagagacaaa gaagtttgaa gaagtagcca aagatcgcgc taacgactag tacgtactta
     2401 taaatgatag gcatagatga agtgggaaga gggtcatggg ctggccctct tgttgtgtgt
     2461 gctgcccgtt tgaataagcc tattaatggt cttaaagact ccaagctgct tagcaaaaaa
     2521 cgccgcgaag agttatttgt acatatagaa aaaaactgtg acattggtgt aggctgggta
     2581 tcagccgatg aaattgatga catagggcta agcgcagcgt tatattatgc tgcagggtgc
     2641 gctttagcag agctcagccc gacatcagaa gaagtagtca tagacggttc tattcagcta
     2701 ttaccagcct atgaagcgag tacggtgcgc attaaggcag ataacactgt accagccgtg
     2761 agtgcggcga gtatttacgc aaaagtcata agagacaact ggatgtgctc tcaagatagt
     2821 atctatgatg cgtatgattt ttggcacaat gtaggttacg gtaccttaag gcatcgaaaa
     2881 gcgatagaac agtacggtat aacacccttg cacagaactt cattttcgct acctagtaga
     2941 atcgtctgat atagttttaa gtaagatgaa cacgacacaa gtaggacgcc aagctgaaca
     3001 ggctgccgta caatggttgc tgcagaatgg gtatcaaata attgatcaaa actggcaaac
     3061 tcgttggtgt gaaattgata ttatcactaa aaaagcaaac gttatatttt ttgtagaagt
     3121 acgatatcgt cggaatgatg cttggggcga tggcatagac tcaatcacac ctaggaaatt
     3181 gcagcaaatg caatttgcag cagagctttg gttaacacag gctaagtgga gtcacgatgc
     3241 atcgttatgc gctatggcgg tggctggtca gccacccgta gtcagtgctt ttatagagct
     3301 gtagaaagcc acgactctac tgctttttga tcgcgctgac gccactctat tttagcagta
     3361 atatctttgg tgcctaaatc tatattacgg gccaatgaag ggatatccgc tagtggtgta
     3421 aagtgttcag cgtaagtatt aagccactct tgtgtagaaa agacggcagc actgtaacgt
     3481 ggatagttat catacgattt atcttgggcg aatacttctg taatccaatg ccagttggta
     3541 accagccact gccatgcgct ggcacggctt gcctgattgc gtaaaagata taccaaaaac
     3601 ctatcaagat cttgtaatct aacaaattgc tgatctttaa gccggctaat cagttttttg
     3661 atacgagtgg tatcttttgt agcacataga gcaccagcaa tatcaagttg tatatcacca
     3721 tttttagttg ctgggtaggc agtaaggaat gaatcaaaca tcgctttgtc accagattta
     3781 ataaccgctg ttagaattgc tgatcgagtg tcaactggta gagcttcaaa atcatcgcaa
     3841 tcattaaata ttttcgttgc tgttttaatg acattaggct cttctgagta tatagctaag
     3901 cctgcaataa tgctgcgaag tttcttgtca ttattggtat cggttacttt agacacgagc
     3961 cctaaacgag tatactgctt ctctattaaa cggtatgtaa gtttttttag agacgactct
     4021 gctgtttcgt taccctctat catgagtctt gtgtcaccga taacaagact aatcacactc
     4081 cacacagact gttcggtctc gtttacgtaa taactcagag aagtcaaggc atctgtaagt
     4141 gaaccaacac cacagcgggc taatagtagt gggtcatgca ataacaataa acgtgcatct
     4201 ggtgagatgc gatgatgttt gacatcgtca taaattgcca atcttgttgc atcagacgtg
     4261 tagtatggca cataatgtcc accgtatatg ttaaaccgag tatgtggttt ttgtacatgc
     4321 accagtttgc gttttgtatc gagaatatcg agcgctgagt tgtctgaaga ccatagcggt
     4381 attgaccaca gtttttcaga tgtatgcatt gtggttgtca gctgatgttg ttctacactg
     4441 agtgtggtat catctaccgg tgtaattttt atgtacggga atcctgactg ggtaatccaa
     4501 ccgtgcataa tggaagcaac atctatacca cttgcagcgc cgagcgcatt ccacaggtct
     4561 gcgccaacag tgttttggta ggcatgatgt ttaaaatagt ttcgtagacc ttttttaaaa
     4621 ttacgatcgc ccacaacgtg ataggccata tataacaatc gtgcgccctt agcatatacg
     4681 atagaaggat caaaaagtgt gctaattgca tcggggtggt gtacaggtgt ggctactggt
     4741 tgcacaccag gcaatacgtc gcgtctaaat gcgctcagag catcgtgggc agcaaagctt
     4801 aacataacgt tccactctgg gtatatagcg tctatcacta agtactccat aagtgtggca
     4861 aaactttcgt tcagccacag atcatcccac cactgcatcg tcacgagatt accaaaccac
     4921 tgatgcgata tttcatgtgc aatcaccgtt gctatatatt ctttagctga ggttgatatg
     4981 gtgtctgggt cagcaataag ggcgatttca cggtaggtga tgagacccca attttccatg
     5041 gcgccactac taaaatccgg aagcgccaca tgatcacatt ttggtaacgg atatgccacc
     5101 ccaaaatatt cattaaaaaa ctcggtggtt tttaccgcta cttctagtgg gaagaccagt
     5161 gattctatgt cgtgatcttt actagaccaa actgtaattt ctgtgccgtt ttctgtttgt
     5221 gtagaaactt tctgcagatt accacacaca aatgcaacta agtaactact catcactgga
     5281 gtcgtttcaa aaataaaggt tgttgaatca tctgtattct gatgacttgt ttgctggggc
     5341 atattactca gcacaattgg ttcatgtgtc gttatttgta atgcgaacgt agctttagct
     5401 tctggttcat ctatacaagg gaacgcttca cgtgcatggt ggctttcaaa ctgggtgccg
     5461 aggataaccg aaccatcatt tgtgtatgct gggtaaatac cgtgcattgc ttttgttata
     5521 gtgccgctaa aatgtactgt cgcagatata cttgtttttt taatgtttgg tatgtgaata
     5581 tctagaatgt cttcgttgat agaaaaatcg caggcggtac tatcaactgt tacacctatg
     5641 atttgcaaat ctttcacatg aagccgtata tggggcgaac ttttttttct atcaccaaat
     5701 acggtaacgg tgccactaaa tcttaggagt gtcgcatcta gagttaaatg aacatcataa
     5761 tgagttgggg taaattcacg atatagtcgg gctacatcag tcaagggttt ctcctttttg
     5821 ctttaattag gttgttttag ctgccactct atggtatcat acgaagtaca ccgttccgac
     5881 catattggtc ggatttttta ctgaagaagg gagtagaaat aaaatggata tcgagacaaa
     5941 acaattagtg atggcagttc gaacaattgc cgaagaaaag aacttaccag aagatgtcgt
     6001 acaagaggct atagaacaag cattagcagc tgcatggcgc cgagattttg gtgacaaaga
     6061 ccaagaagtg cggtctgtga ttaatatcaa tgatggttcc gtaacagtat ttattacaaa
     6121 aactgtagta gataatgtaa cgaatgagca cttagaaatt tcactcaaag acgcacaggc
     6181 aattcgtaaa gatgtcgctc ttgatgaaac ggtagagata gaagaaaagg tagaaaactt
     6241 tggacgggtg gctgcccaaa cggcaaaaca agtcatacta caacgattac gtgaagccga
     6301 gcgagaagtt gtattggctg aattcgaaga caaaatccac accgttctaa atgctatcgt
     6361 aactcgcatt gagggtcgga tggttcgaat tgagattaat cgtgctcagg gaattttacc
     6421 accgagcgaa caaatacaag gagagcgtta ccatccgggc caacgtctga aggtgtatct
     6481 aaaagacgtt gagcgtggtt ttaaaggtgc ccaactggta gtaagccgcg gttgtcctga
     6541 atttattgaa cacttgtttc ggaacgaagt acctgaaatg gaaggtggcg ctgtagaaat
     6601 taaaggcata gctcgcgaag caggtgtacg aactaaaata gccgttgcca gtaatttgcc
     6661 aggtgttgac ccagtgggaa cttttgttgg tggtcatggg acgcgtgtac aagcagttat
     6721 gagtgaaatt ggtgaacaag aaaaaattga tatcgtagtt tttgcaagcg ataccaagga
     6781 gtacattatt aatgctctta gcccaaccaa agtcgtaagc gttgaactta acgaaaatgc
     6841 gaaaaaggcc aaagtaactg tagaagaaga ccaactatcg atagcgattg gcagaagtgg
     6901 ccaaaacgta cgacttgcca gtaggctcac tgggtatgat attgatgttg taggagataa
     6961 gccagcagct gctgaagtac cagaaaagaa agacactgtc gtcggagcct ctaacgctcc
     7021 taaactcaag aaaaaaagtg atttagagtc tagtctcctt gaggcaatcg aagaacacgg
     7081 cgaataaaag aggtctgtat tagacgtata cgagattgta attagcactc ttgattaatg
     7141 agtgctaatt tcatataatt aaatggtatg attgctacac tattaatatc tagagaaaga
     7201 ataagggtat tgtcatgaat gagtcacaag atatgcaaga atttctgaac cacctgactg
     7261 ataatgccct tatgagccta aaacatgctg atcatgttgc acgtacgcaa ggttcggcat
     7321 atgtcggcac tgaacacata cttctgggag tattggctca agacaattca gtagcttcta
     7381 aaatactcac cacagcaggc gtttcctatg agagagccca gctagccctt aacctaactc
     7441 ctaaaaatct cgtcattaat cttggtgcga aaggtctcag cgaaaccgct aaattagcac
     7501 tcaaggtgag ctgggattac gcacaagatt acggccaaga catgtgtggg accgagcaca
     7561 ttgtgtttag tattttgcaa caaaaaaatt cacgtgcgac agtactgctt agagatatga
     7621 atatcaatac tgataaattg ttgaattctt ttgatgactt tttgcagaag cagcagtttg
     7681 attcagacca atctcgcaaa cgcacggcaa ctaagtc
//
LOCUS       DAOPLS010000014         7648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_10223_length_7648_cov_4.830238, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000014 DAOPLS010000000
VERSION     DAOPLS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10223_length_7648_cov_4.830238"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKV52_00670"
     CDS             complement(<1..386)
                     /locus_tag="PKV52_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD98758.1"
                     /translation="MISLRKENKGFTIIELLIVIVVIGILAAIGFVAYGNVTKSARDS
                     DRQADASALAKKAEEYYASNGQYPGDIATLTAMEGVDAKTTTSPSGNAAQGGGSTLGA
                     CTTEPDSKANDFYCYIVVTDQSQMEIR"
     gene            complement(481..1695)
                     /locus_tag="PKV52_00675"
     CDS             complement(481..1695)
                     /locus_tag="PKV52_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD98759.1"
                     /translation="MAKFSYKGINQSNTVIKGTIDASSKQAVVDTLKKQGIKPLSVRQ
                     DAGFKLFSLSSKRVKTKDITIFTRQLSTLISAGVPLNRSFYTLQEQTDNKYFQVVIGD
                     IAKQIEGGASIGDALKKYPNVFDEVYINMVIAGEAGGILDKILKRLATQLEKNQAIKK
                     KIKSATTYPKVVFGITIIAFIVVMNVIVPKIGAMLLELGGEDAQLPILTRVMIGISDF
                     SKQYAVFIVIAGLIAAYFIRRYIKTPKGKLKYHTLLLRIPVLKNVITKVAISRFARTF
                     SSLMSSGVSVIEAIEITAGSIGNKVLENELLAAAKDVKAGKQLSEPILNSPHYPSIVG
                     QMLAIGEETGETDSILVKIADFYEEEVDATIESLSSILEPLMIVILGGMVGLVAASVM
                     GPLASLSGSITK"
     gene            complement(1705..3501)
                     /locus_tag="PKV52_00680"
     CDS             complement(1705..3501)
                     /locus_tag="PKV52_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPD98760.1"
                     /translation="MRVSDKLVEDLLAKSEKVTPDQIASLKEQEKNEHKPLQDIVLQA
                     NLISEKDLTSAFAEQIDVPFVEIIAKDIEKETLHLIPERIARQYGVVVYGIDKNGTKL
                     LAMEDPDDIQAISFLQKQLGNDVRISIATKTNILQAIDQYRENISSEITEIISGEDED
                     SESDEVNEEDLAEDSPVAQTVNLLIEGAIKSGASDIHIEPRENYVLVRYRVDGILREV
                     NKLPKKVQAALVSRIKILSNLKIDERRAPQDGRFKIGISNLIYALRVSTLPITDGEKV
                     VMRILSESTKALTLEQLGYWGPSLNTVNQAITQPHGMILVTGPTGSGKSTSLFSILSL
                     LNNPTVNISTIEDPVEYKIPGTNQTQVNVQAGMTFVNGLRALLRQDPNIIMVGEIRDS
                     ETAGLAVQAALTGHLVFATLHTNNAATCLPRLLDMGIEPFLIASTVRAVVGQRLVRRL
                     CQHCKQSFKPDDKMIKTIAQSFGIKDTSVMQYIYDLEAQAIAGGIGVNQEASSKTSSS
                     SAKGITRLWKANLEGCDECGHVGFKGRLGIYEALGNSTELQKLIISNATSGVINDQAI
                     KEGMVEMLIDGFVKALRGETTIEEILRVTAEA"
     gene            3661..4677
                     /gene="recA"
                     /locus_tag="PKV52_00685"
     CDS             3661..4677
                     /gene="recA"
                     /locus_tag="PKV52_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPD98761.1"
                     /translation="MAAKKEQEKSTAQSGKSQALDLAVEQIKKQFGQGSIMQLGADYK
                     VDVPTFSTGSLGLDIALGGGIPKGRIIEVYGPESSGKTTLTLHGVAEIQKQGGTAAFI
                     DAEHALDPSYAQRIGVDVANLLISQPDNGEQALEIVETLVRSNAVDLIVVDSVAALVP
                     QAEIEGDMGDAHMGLQARLMSQALRKLTGVINKSKCTVIFINQLRMKIGVMFGNPETT
                     TGGNALKFYASVRMDIRRISQIKTADSVIGNRVRVKVVKNKVAPPFREAEFDIMYNVG
                     ISREGETLDLAVHNDIVGKSGAWFEYKDAKIGQGREAAKQYLMDNPKVLAEIDKKVRD
                     VALA"
     gene            4681..5286
                     /locus_tag="PKV52_00690"
     CDS             4681..5286
                     /locus_tag="PKV52_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPD98762.1"
                     /translation="MVITKITLQQKDTQRANIFIDGTYELSLTIDQVLEQKLKVGVNV
                     DELALKRLRKLSEDGKVQARALEWCLSRPRSSKELRDYLYKKGASQELSDSICHTFAL
                     KKYQDDDYFTKWWIENRVRKNKSNLSIKTELKQKGINDELITKNLQVEVTPKIRLKNL
                     IVLKRLQTKYPDENKLKQYLAGKGFYYSDIVEVLAELKEGA"
     gene            complement(5238..5792)
                     /locus_tag="PKV52_00695"
     CDS             complement(5238..5792)
                     /locus_tag="PKV52_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98763.1"
                     /translation="MLFFGSSFNGMPLMSLRSAAKIGTITEPVINPHNLHIDGFYAQA
                     MNGQRDGIVVDIDVRDLSPTGMIIDDHEDIAEQEELVRLQPIITINFKLIGKNVYAGS
                     SKIGRVEDYVVDTKSLFIQKLYVQPSLLKLTSSQMIFDRQSIREVTDTKIVVSGPEVK
                     DTQKVSSRFQAPSFNSASTSTISE"
     gene            5854..>7648
                     /locus_tag="PKV52_00700"
     CDS             5854..>7648
                     /locus_tag="PKV52_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPD98764.1"
                     /translation="MIKRAMTLLLATTLCIATSSTAFAEDTPQSKPTENNVIITELQT
                     NGEGTGTTLQEFIELYNPLSEPVNMLGWSVVYKNSTGKETVVHTFTEPFLLLPSSYMI
                     GKNEFTVESYLPDVLADFTYSYGSSGLAATAGSVMIKNQTDIVVDELAWVNTMSFIDN
                     NTLNGLIGGMSAQRKCDAEQYFIDTNTVANDYFIGLPTPLSATCLSPSEDTQDNSQTD
                     TIEPLVPVEQKPPATAPRMRLPLLINELFIDPIAPLTDSKDEFVEITNHNETAVDISG
                     YTITAGTTTKYKYTFPSGSIIGPNGYLSITSAATTIALTNSGGTVQLADDLGTVVDSV
                     QYTAAVPGAAWARNNAGTWQWTTAPTNGSANVIIPPPQAQAKTKKTSASAAKKKSTKK
                     TTKKKTNFSVAPPIQINEVFPDPKSPQTDAKDEFIELYNPHPTAINITDYTISAGATR
                     KYTYTFPEGSVILPQSYIVVGSDSTSLSLTNSGATVQLINNFDKEIDTVTYDKAISGQ
                     SYARDDFGKWQWTSTVTKNEKNIITASASKKSSKTSVAGIVAGTDSKPLAPAPQPLPT
                     WVLAMLGFGAVCYAAYEYRFEARNIFYKFKAH"
BASE COUNT         2350 a         1528 c         1512 g         2258 t
ORIGIN      
        1 cgaatttcca tttgtgactg atctgtgacg acgatataac agtaaaagtc atttgctttg
       61 ctatcaggtt ctgtagtaca tgcgccaaga gtgctgccac cgccttgtgc tgcgttaccg
      121 cttgggctag tcgtagtttt agcgtccaca ccttccatag ctgtaagtgt agctatgtca
      181 ccaggatatt ggccattact tgcgtaatat tcttctgcct ttttagccag cgcgcttgca
      241 tcagcttgtc tatctgagtc acgggcgctc tttgtgacat taccatatgc tacaaaacca
      301 attgcagcca aaattccaat tacaacaatc acaatcagaa gttctataat tgtgaaacct
      361 ttattttctt ttcttagaga gatcatataa atcccaccct cctagttagt tattagttgc
      421 gatgctattt gtttagtcat cttgcttatg attttatatc tatggcttaa cagttgtcaa
      481 ctattttgta atactaccac taagggaggc aagcgggccc ataacacttg ctgctaccaa
      541 gccaaccatg ccgccaagaa tgacaatcat caaaggttct agtatagagc tcagtgattc
      601 tatcgtcgcg tctacttctt cttcgtaaaa gtcagcaatt ttgaccaaaa tactatcggt
      661 ttcacctgtc tcttcgccaa ttgcgagcat ttgcccaaca atagaagggt agtgaggact
      721 attgagaata ggttcagata attgtttgcc agcctttaca tctttagcag cggcaagaag
      781 ctcattttct aatactttat tgccaatact gcctgctgta atttcaattg cttctataac
      841 gctcacacca gaagacatca aactagaaaa agtacgcgca aaacgagaaa tagctacttt
      901 tgtgataacg tttttaagca ccggaatacg cagcagtaaa gtgtgatatt ttaactttcc
      961 tttgggtgtt ttaatatatc tacgtataaa ataagcagca ataagaccgg caatgactat
     1021 aaaaacagca tattgtttac taaaatcact aataccaatc attactcttg ttaagattgg
     1081 tagctgggca tcttcgccac ctaactccag taacatggca ccaattttcg gtacaatgac
     1141 attcataacg actataaatg cgatgattgt tatgccaaac actactttcg ggtatgttgt
     1201 agcgcttttg attttctttt ttatagcctg atttttttct aattgtgtag cgagcctctt
     1261 aagaatctta tctaatatac caccagcttc accggcaatc accatattaa tataaacttc
     1321 gtcaaacaca ttagggtatt tttttagagc atcgccaata cttgcgccac cttcaatctg
     1381 ctttgcaata tcgcctatca ctacctgaaa atacttatta tcggtttgtt cttgcagggt
     1441 atagaaagat cgattcaatg gcaccccagc tgaaataaga gtagataatt gccgtgtaaa
     1501 gatggtgata tcttttgtct taactcgttt gctcgaaagt gaaaagagtt taaatccagc
     1561 atcttgccgg accgaaagtg gttttatacc ttgtttttta agtgtatcaa caacggcttg
     1621 ttttgatgat gcgtctattg ttcctttaat gacggtattt gattggttga tacctttgta
     1681 agaaaatttt gccatatatt cttgctatgc ctctgccgtg acacggagaa tttcttctat
     1741 agttgtttca cccctgagag cttttacaaa accgtctatg agcatttcta ccatgccttc
     1801 tttaattgcc tgatcattga taactccact ggttgcatta ctgatgatga gcttttgtag
     1861 ctctgtcgag ttaccaagtg cttcataaat accaagtcgc cccttaaaac caacatggcc
     1921 acattcatcg cagccttcta ggtttgcttt ccagaggcgt gtaatacctt tagcagaaga
     1981 tgatgatgtt ttagatgatg cttcttgatt cacacctata ccacctgcaa tggcctgtgc
     2041 ctcgaggtca tagatatact gcataacaga agtgtctttg ataccaaaag attgtgcaat
     2101 agtttttatc attttgtcat cgggtttgaa tgattgcttg cagtgttgac acaatctacg
     2161 cactaaacgc tgccctacaa cggcacgcac agtgctggcg ataagaaaag gttcaatacc
     2221 catatcgagc aaacgcggta aacaggtggc agcattatta gtgtgtaggg ttgcaaaaac
     2281 taagtgacca gttagcgctg cttgcaccgc caacccggca gtttcgctat ctctaatctc
     2341 accaaccata ataatattgg ggtcttggcg caacagtgct cgcaaaccat taacaaaggt
     2401 catacctgct tgcacgttaa cttgggtttg gtttgttcct ggtattttat actcaaccgg
     2461 atcttcaatg gtagaaatgt tgacggttgg gttatttagt aagcttagta tgctaaacaa
     2521 acttgtagat ttaccggaac cagttggccc cgtgacgagt atcatgccgt gcggctgagt
     2581 aatagcttga ttgaccgtgt tgagtgaagg accccagtaa ccaagctgtt ctaaggtaag
     2641 tgctttggta gattcagata aaatacgcat aacaaccttt tccccgtctg taataggtag
     2701 ggtagaaaca cgtagggcgt aaataaggtt actaatacca atcttgaatc gtccatcttg
     2761 cggagcccgg cgttcatcaa tctttaaatt agataaaatt ttaatacgcg acacaagggc
     2821 tgcctgtact ttttttggaa gcttattgac ctctcttaaa attccgtcaa ctctataccg
     2881 tactaataca taattttctc ttggctcaat atgaatgtct gatgcaccgg atttaatagc
     2941 accttcaatt aacaaattta ccgtttgagc aactggtgaa tcttcggcga ggtcttcttc
     3001 attaacttca tcactttcgc tatcttcatc ttcgccagaa ataatttcag taatttcgct
     3061 actaatattt tcccggtact gatctattgc ttgcaagatg ttggtttttg tagcaattga
     3121 tattcggaca tcattaccga gctgtttttg caaaaaactg attgcttgga tatcgtctgg
     3181 atcctccatg gcaagtagtt ttgtgccatt tttatcaata ccatatacca caacaccgta
     3241 ttgacgagct attcgctctg gtattaagtg gagcgtttct ttttctatat ctttggcaat
     3301 gatttctaca aacggaacat ctatttgttc tgcgaatgca ctcgtaagat ctttttctga
     3361 aataagatta gcttgtaaaa caatatcttg cagtggtttg tgctcatttt tttcttgttc
     3421 ttttagggag gcaatttgat caggtgttac cttttcactt tttgcgagca gatcttcaac
     3481 taatttatca gatactctca ttgaattctc cgtgagtaac aagatagcaa catatgcgaa
     3541 tatttgtttt catgcttacg cttatggtat cgtatctagt tatatatagg caagtaatac
     3601 gccggtggtt ttgctataat ggcaggtagt attactaaca cgtggagggt tcttactata
     3661 atggctgcaa aaaaagagca ggaaaaatcg acagcacaga gcggcaaaag tcaggcattg
     3721 gatctcgccg ttgaacagat aaaaaaacaa tttggtcaag gttcaattat gcaacttgga
     3781 gctgactata aggttgatgt accaacattt tctactggta gcttagggct agatatagca
     3841 ttgggcggtg gaattccaaa aggtagaatc atagaagtct atgggccaga gagtagtggt
     3901 aaaacaactt tgacactgca tggtgtagca gaaatacaaa aacaaggtgg tacagctgca
     3961 tttattgatg cagagcacgc actagaccca tcatacgcgc agagaattgg tgtagacgtt
     4021 gctaatttat tgatttcaca gccagacaat ggcgaacaag cactcgaaat cgttgaaaca
     4081 ctcgtacgta gtaacgcagt agacttaatc gtggtagatt cagttgctgc tcttgtacca
     4141 caagcagaaa tagaaggtga tatgggtgat gcacacatgg gtttgcaagc tcgcctaatg
     4201 agccaagctt tacgcaaact gacgggggtt attaataaaa gcaagtgtac cgtcattttt
     4261 attaaccaac ttcgtatgaa gataggtgtt atgtttggca acccagaaac cactacgggt
     4321 ggtaacgccc taaagtttta cgcatcggtg cgtatggata tacgccgcat tagccagatt
     4381 aaaaccgcag actcagtaat tggtaacagg gttagagtga aagtggtaaa aaataaagtt
     4441 gctccacctt ttagggaagc tgagtttgac attatgtata acgttggcat aagtagagaa
     4501 ggtgaaacac tagacttagc agttcacaat gatatcgttg gtaaaagtgg tgcatggttt
     4561 gaatataagg atgccaagat tggacaaggc cgtgaagcag caaagcaata cttaatggac
     4621 aaccctaaag tacttgctga aattgacaaa aaagtccgag acgttgctct tgcgtagtaa
     4681 atggtaatta cgaaaatcac tctgcaacaa aaagatacac aacgggcgaa catctttata
     4741 gatggcacgt atgagctatc attaactatc gatcaagttt tagaacaaaa gcttaaagtt
     4801 ggcgttaatg ttgatgagct ggcgttaaaa cgtttacgaa agctttctga agatggcaag
     4861 gtgcaagcta gggcactaga atggtgtttg agccgaccaa gatcttcaaa agaattacgt
     4921 gattatttgt ataaaaaagg tgcaagccaa gaacttagtg attctatctg ccatacattt
     4981 gccttaaaaa aataccaaga cgatgattat tttactaagt ggtggataga aaacagagtt
     5041 cgtaagaata aaagtaatct tagtattaaa actgagctaa agcaaaaagg cattaacgac
     5101 gaactaatta caaaaaattt acaagtagag gtaacaccca aaattcgcct taaaaattta
     5161 atagttttaa agcgcttaca aacaaaatat ccagatgaaa ataagcttaa acagtattta
     5221 gctggcaaag gtttttacta ttcagatatc gtagaagtac tcgccgagtt aaaagaagga
     5281 gcctgaaacc tgctacttac tttttgagta tcttttactt ctggaccaga cacgacaatt
     5341 tttgtatcag taacttctct tatgctttgt ctgtcgaata tcatttggct tgatgtaagt
     5401 tttagcaatg acggttgtac atatagtttc tgaataaata aactttttgt atctacaacg
     5461 taatcttcta ctctaccaat tttagaagac ccagcgtaaa catttttacc tatcaattta
     5521 aagttgatag taataatagg ctgtaagcgt acgagttctt cttgttctgc aatgtcttca
     5581 tgatcatcaa taatcatccc tgttgggcta aggtcacgta cgtcaatatc tactacgatg
     5641 ccgtcgcgct gcccattcat cgcctgggca taaaagccat ctatatgtaa attatgtggg
     5701 ttgattacag gttcagtaat agtaccaatt tttgctgcgc tacgtaggct cataagaggc
     5761 ataccattaa aactactacc aaaaaataac atattgtctt tattgtaata aaaaatgctt
     5821 gtgttagcga attactatcg tacaatagtg ctcatgataa aacgtgcaat gacgttacta
     5881 ttagctacaa ccctatgcat tgcaacatcc agcacagcct tcgcagaaga tactccacaa
     5941 tcaaaaccta cagaaaacaa cgttatcatc actgagctac agacgaacgg ggaaggtaca
     6001 ggaaccaccc tacaagaatt tattgaacta tataatccac tatctgaacc tgtaaatatg
     6061 ttagggtgga gcgtcgtcta caaaaactct acaggaaaag aaaccgtagt acatacattt
     6121 acagaaccat ttttgctttt gcctagcagc tatatgattg gtaaaaacga atttaccgta
     6181 gaaagttacc tcccagatgt tttagcggat tttacctata gctatgggtc aagcggactg
     6241 gcggcgactg ctggtagcgt tatgataaaa aatcaaacag atatagttgt agacgagcta
     6301 gcgtgggtca acacgatgag ttttatagat aacaatactc tcaacggttt aataggggga
     6361 atgtctgcac aaagaaaatg tgacgccgaa caatatttta ttgatacaaa tacagttgct
     6421 aacgactatt tcattggctt accaactcct ttgtctgcga catgcctatc gccatcagaa
     6481 gatacccaag ataatagtca aacagatact attgaaccat tagtgcctgt ggagcagaaa
     6541 ccaccagcta cggcgccgcg tatgcgttta ccgctactta ttaatgagtt atttattgac
     6601 cctatagctc cgttaactga tagtaaagat gaatttgtag aaatcaccaa ccataatgaa
     6661 actgctgtag atattagtgg ctatacaatt accgccggta cgacaacaaa atataagtat
     6721 acgtttccga gtggcagtat cataggccca aatggctatt tgagtataac ctctgcggcg
     6781 actacaattg cattaacaaa tagtggtggc acagttcagc tggcagacga tctgggcact
     6841 gtcgtagata gcgtgcaata tactgcagca gttcctggag cagcttgggc cagaaacaat
     6901 gccggaacgt ggcagtggac aactgcacca accaatggtt cggcaaatgt catcattcca
     6961 ccgcctcaag cacaagctaa aaccaaaaag acgtctgcct ctgcagctaa gaaaaagagt
     7021 acgaaaaaaa caaccaaaaa gaaaactaac ttttcagttg cacctcctat acaaattaac
     7081 gaagtatttc ctgatccaaa atcacctcaa accgatgcaa aagatgaatt tatagaattg
     7141 tataatccac acccgacggc gataaacatt actgattaca caattagtgc tggagcgacc
     7201 cgtaagtata cctacacttt tcctgaaggc tctgtgattt tgccacaaag ctatatcgtt
     7261 gttgggtctg attctacgtc attgtcgtta acgaacagcg gagcaacagt gcagcttata
     7321 aataattttg ataaagaaat agatacagta acatatgata aagcaatcag cggtcagtca
     7381 tacgcaagag acgattttgg taagtggcaa tggacatcaa cggtcacaaa aaacgaaaaa
     7441 aacataatta ctgcttctgc ctccaaaaaa agtagtaaaa cctcggtggc aggtatagtt
     7501 gcaggaacag acagtaagcc cttggcgccc gcaccacagc cgttacctac atgggtgctt
     7561 gcgatgttag ggttcggcgc ggtatgctat gcagcgtatg agtaccgatt tgaagcgaga
     7621 aatattttct acaagtttaa agcacaca
//
LOCUS       DAOPLS010000015         7442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_10587_length_7442_cov_3.853120, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000015 DAOPLS010000000
VERSION     DAOPLS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10587_length_7442_cov_3.853120"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PKV52_00705"
     CDS             complement(<1..943)
                     /locus_tag="PKV52_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98765.1"
                     /translation="MNTKKHPKQASKKSAFKRAFAAFRSLSVWKKSLLIIIIFCIAFV
                     AQAYAIGYWYQKKHSSEPITFGVTFIPNYARFFELDPQETMLALRDDLGFKRFRLVSY
                     WKDIEKSPGQYDFSELDWQFEKVNEVGGEVSLAIGLRQPRWPECHAPQWVKAEDKDVW
                     YPQLKQFMQAVIERYGDNPALTSYQLENEFFLGVFGECGHYGAERERLIDEFNFVKNL
                     DPNTPIILSYANNYFGVATGQPQPDQVGVSVYKRVWEQTVTKRYFEYPFPSWYYSWRA
                     GLQEILTGKDSMLHELQAEPWTPSDVKSVSTEEQYKSM"
     gene            991..1824
                     /locus_tag="PKV52_00710"
     CDS             991..1824
                     /locus_tag="PKV52_00710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPD98766.1"
                     /translation="MSAKPPTISVVVPVYNDEPYVKACLGALQAQTVTPHEILFVDNN
                     CTDGSMAIARRYKNVKILHEPKQGLTYARNTGLNMATGDILLRIDADTVLPPTYIERL
                     QQLAVAHPDVAGFSGYGVSRYEFIPKISLLWAWSYFAFTKAYLAYPVLWGANMAIRKQ
                     YWHKAKKYLINNDMAVHEDQDVSLAIASVGGRVLVTRQLTVSVQMKAMFEYARYRKYG
                     LMLQLLKRLDKEHPRYQLATRVPPLAWPKRALYWVATVWAVYAFYLVTFAYELMRKLR
                     R"
     gene            complement(1821..2432)
                     /locus_tag="PKV52_00715"
     CDS             complement(1821..2432)
                     /locus_tag="PKV52_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPD98767.1"
                     /translation="MHSSKAPIQTPPKSRAKLLLSSVAIVGGLAAIVLISGIVRVLPQ
                     QKSVELPPKPGFYLVSHVFDGDTISVKMDGREEKVRLIGVDTPETIKPNSPVECYGVA
                     ASNNLKSLLSAKTVRLESDPINQNRDRYDRLLRYVYTQEGTLVNEQIIREGFGFAYLS
                     FPFTKAEEFRLAQVDARQNNRGVWGGQCDITNPNGDRPKTNEL"
     gene            2522..3142
                     /gene="rsmG"
                     /locus_tag="PKV52_00720"
     CDS             2522..3142
                     /gene="rsmG"
                     /locus_tag="PKV52_00720"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPD98768.1"
                     /translation="MDNILKHFLHLSAVQQQQLAALGALYHNWNAKINVISRKDIDLL
                     YPNHILHSLAIAKYIEFSAQDRVLDAGTGGGMPGIPLAILFPNTQFVLVDSTRKKLQV
                     VDAIAAELGLSNVQTEHIRLEEIHDQYDYVVSRAVTRLDVMWGWVHQRIRQSSQNKVP
                     NGLLYLKGGDISGELPNNCRVQKIPLQSLINEPQFVEKALVHICKK"
     gene            complement(3581..3718)
                     /locus_tag="PKV52_00725"
     CDS             complement(3581..3718)
                     /locus_tag="PKV52_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98769.1"
                     /translation="MSYEAIANWHQKHPIPHEIQLALGSTGTTRQLEISLSADSDLEQ
                     E"
     gene            complement(3784..4353)
                     /locus_tag="PKV52_00730"
     CDS             complement(3784..4353)
                     /locus_tag="PKV52_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPD98770.1"
                     /translation="MSFEDFKSKDSVVVVYTGEGKGKTSASIGLMARALGANWKVAYI
                     QFIKHWHVNEHTFIENITPIYGDNLLFMKGGAGFYNAGELSAKNVTENEHKAIALKTY
                     TAALQAATSGDYDLVICDEINNAVHDGLLTVQHLRELLEEHHPKTSICVTGRDFPNEL
                     MHFTDISTNMTKLKHHFDDKYLANPGIDY"
     gene            complement(4381..5004)
                     /locus_tag="PKV52_00735"
     CDS             complement(4381..5004)
                     /locus_tag="PKV52_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98771.1"
                     /translation="MRGQGMAKSIYGTFVPPAELLLEYDNNRHRAHIPMPRDSTETIE
                     PAPPAHYSAELISTSLDGPTSQDITRFLDTYYTVESAPKIGAFLLRLLEITRAIPKEH
                     VIEDTKQRHDQIMAAEQLLIDYELFIDETTGMFDQKRFNELITQELGEHIIALYPITR
                     ILQRAVITAHEQGTADKQHSLTNIYRLVLIAIAKQRGIYNSDQTTKP"
     gene            5137..5886
                     /locus_tag="PKV52_00740"
     CDS             5137..5886
                     /locus_tag="PKV52_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HPD98772.1"
                     /translation="MIKQRHVAALLVEFFGTLTLALVVLSVSLYRFPFFTALAAGVTV
                     AVFVSAFAKISGGHVNPAVTVGMFAIRQITALRAVGYIAAQMLAGFAAWQLYEYLTER
                     ALTNATTATVEWNVAAAEALGAFIFGLALTGAVAQKLKGYQLAFTAGAGLFIGLTVSG
                     LASNSLLNPAVAVAVRSFDPVTYVGAPVVGYLVAAALVAYVVVPMFGKKEVEKTTAVA
                     AAKPSVAKTATKKAPAKKPAAKKKAPAKKKK"
     gene            complement(5947..>7442)
                     /locus_tag="PKV52_00745"
     CDS             complement(5947..>7442)
                     /locus_tag="PKV52_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPD98773.1"
                     /translation="VETASSPTIASPFKSYYFSDTNLQNKPQAYLELLAALSAVRSSL
                     RSYKPDITHSLQDYIHFCDLAKKAHVSIPVKMMHATGKDPVNLLSAHKSKGLEFDTVY
                     ILSATQSIWNSKGRGGGISLSPNLLSIAHSPNPDDNLRLFYVAMTRAKRHLFMSSFMT
                     SDTGKEMIPYAPLEEKTVQAIIQSPVEPMAMPSTPHDILFRAERHWHDKHLSVPQATL
                     KDLLQDRISRYTLSATHLNNLLDVPNGGPHKFFLHNFLQFPSAMSPSASFGSAMHSTL
                     EYMHSYVVKHGSLPALAKSESFMASSLQRKGLAPNEYDKYHTQAINALNALYAQRSHE
                     FTPKQLPEQDFRRQGVRIGDALLTGKIDVLTVTKHSATVTDYKTGTSSTTWNAQGQTN
                     YAKQKLHRYKQQLYFYKLLVDGSRTWGEQGIRATKGELVFVEPNQQGSLDSLSLDITD
                     EQELTRLRKIIASVWRCIVNLDFPDISVFSEDAKGITEFEDWLIANK"
BASE COUNT         2027 a         1501 c         1620 g         2294 t
ORIGIN      
        1 ccattgattt atattgttct tccgtactca ccgacttaac gtcacttggc gtccagggtt
       61 cggcttgaag ttcgtggagc attgaatctt tgcctgttaa gatttcttgt agccccgctc
      121 gccagctgta ataccacgat ggaaacggat attcaaaata gcgtttggtt acggtttgtt
      181 cccagacgcg tttatacaca gatactccta cttgatctgg ctgtggttgg ccagtcgcaa
      241 caccaaagta gttattggca tagcttaata ttattggcgt attagggtct agatttttca
      301 caaaattaaa ttcgtctatg aggcgttctc gttcggcacc gtaatgaccg cattcaccaa
      361 atacacccaa gaaaaactca ttttctagtt gataacttgt aagggctggg ttgtcgccgt
      421 atctttctat gacagcttgc ataaactgct ttagttgtgg gtaccataca tctttatctt
      481 ctgcttttac ccactgtggt gcgtggcact ctggccaacg tggctgtcgt aaaccaatcg
      541 ccagcgacac ttcaccgccg acttcattga ctttttcaaa ttgccaatct agttcactaa
      601 aatcatactg gcctggtgac ttttctatat ctttccagta gctaactaac cgaaagcgtt
      661 taaaaccaag gtcatcgcgc agggcgagca ttgtttcttg tgggtcaagc tcaaaaaagc
      721 gagcgtagtt agggataaaa gtaacaccaa atgttattgg ctcgctactg tgcttttttt
      781 ggtaccaata acctatcgcg taggcttgtg ctacaaaagc tatacaaaaa atgataataa
      841 tcagtaacga tttcttccat acagaaagtg acctaaatgc agcgaacgcg cgtttaaagg
      901 cacttttttt acttgcttgc ttgggatgtt ttttcgtatt catagtggta atacctatta
      961 ttatacatgg tacaataaga gctaacgata atgagtgcaa aaccaccaac tatatctgtt
     1021 gttgtaccag tgtataatga cgaaccgtat gtcaaagcgt gcttaggtgc gttgcaagct
     1081 caaactgtta cacctcatga aatactattt gtagataata attgcacaga tggttctatg
     1141 gcaatcgctc ggcgctataa aaacgtaaaa atactgcacg agcctaaaca gggcttaact
     1201 tatgcgcgta atactgggct taatatggca actggtgata ttttgctgcg tatagacgct
     1261 gacacagtat taccaccaac atatatagaa cgtctacagc aactcgcggt tgcgcaccct
     1321 gatgtggctg gatttagtgg ctatggcgta tcgcgttatg aatttattcc aaaaatatca
     1381 ctcttatggg cgtggagcta ctttgcattt acaaaagcgt acctcgcata ccctgtgcta
     1441 tggggggcga atatggcgat ccgcaaacaa tactggcaca aggcgaaaaa atacctcata
     1501 aataacgata tggctgtgca cgaagaccaa gatgtgagcc ttgctatagc cagtgttggc
     1561 gggcgggtct tagtaacgag gcaattaact gtatcggtgc agatgaaagc gatgtttgaa
     1621 tatgctaggt atcgtaagta tggactgatg ctacaacttt taaagcgttt agacaaagag
     1681 cacccacgct accagcttgc tacacgagtg ccgcctttag cgtggccaaa acgggctcta
     1741 tattgggtgg cgacggtgtg ggcggtttat gcgttttatc ttgtaacgtt tgcatacgag
     1801 cttatgcgta aactacgccg ctaaagttcg ttcgtttttg ggcggtcacc attagggttg
     1861 gtaatgtcac actgcccgcc ccaaacgcca cgattgtttt ggcgagcatc tacttgggcg
     1921 agacggaatt cttcagcttt tgtaaatgga aaactaaggt acgcaaagcc aaatccctcg
     1981 cgaataattt gctcatttac tagtgttccc tcttgcgtgt acacatagcg aagtaggcgg
     2041 tcatagcgat cacggttttg gttaataggg tcagattcta gccgtaccgt tttagcacta
     2101 agcagtgact ttaagttatt actggcagca acaccatagc attctacggg gctgttaggt
     2161 ttgattgtct ctggtgtgtc cacaccaata agtctgactt tctcttcacg accgtccatt
     2221 ttgactgaaa ttgtatcacc atcaaatacg tgactcacca aataaaaacc tggcttgggt
     2281 ggcagctcta cacttttttg ttgaggtaat acgcggacta tcccagatat gagtactatt
     2341 gcagcaagtc cccctacaat cgctacgcta ctaagcagca gctttgctct tgattttggg
     2401 ggtgtttgaa taggcgcttt agaagaatgc attgattata tcttacggta gggacaaatg
     2461 agctacaata ggtacagtta cgcaaaggcg tgcgtatatc tttgcaaagg aattaaaata
     2521 tatggataac attctaaagc attttctgca cttgagtgcc gtgcagcagc aacagcttgc
     2581 agcgttaggt gcgttatatc ataattggaa cgcaaaaatt aatgttattt ctagaaaaga
     2641 tatagacctg ctttacccaa atcatattct gcatagtttg gctatcgcaa aatatataga
     2701 atttagtgcc caagatcgtg tgcttgacgc aggcacgggt ggtggcatgc caggtatccc
     2761 cttggcgata ctgtttccca acacccaatt tgtacttgta gatagcacgc gaaaaaaatt
     2821 acaagtagta gatgctattg ctgcggagct aggactttcc aatgtacaga cagaacatat
     2881 acgtttagaa gaaatacatg atcaatatga ttacgttgtt tcgagagcag ttacgcggct
     2941 tgatgtcatg tggggatggg tgcaccagcg catcaggcaa tcgtctcaaa ataaggtacc
     3001 caacggcctg ttgtatttaa aaggtggcga catttcaggt gagttaccga acaattgtcg
     3061 tgttcaaaaa ataccactcc aatcgttgat aaatgaacca cagtttgtcg aaaaagcgct
     3121 cgtacatatc tgtaaaaagt aattagtaca acaatatagc ttacaaaaaa agtcgaggct
     3181 ccgcgaggct ctcgacttat tttgaccctt tttgttctgt tagttagtgt agcgtagcgc
     3241 ttatgctgag gtcaagtatt tgtagtatat ttagcaacaa aagactaaaa aaagagcagc
     3301 cgtgggcgta ccatgctgtt cactagaatc taagtagcaa aggagggagc tacataggcg
     3361 tgtacgtgtg caatgaacgc gtgactgctc tttggtaagg tttcatagtt aaatcatgac
     3421 atttgcatct tcactgctcg taaaacatga ctacggcgtg caagcaaata catcatagat
     3481 tagtctaaaa ccctccgtct ggtagtaatc tcccaaaaag aattactact ctactatagt
     3541 agctgctttg gcacttacgt gtcaagagtg ctaatgtgtc ttattcttgc tctaaatcgc
     3601 tatcagcgct cagcgaaatt tctagttgcc tagtagtgcc agtgcttcca agtgcgagct
     3661 gaatttcgtg cggtataggg tgtttttgat gccaattggc aattgcttcg tacgacataa
     3721 tactccttaa tacttttata attactaatt tttttactat aacatatggt acttgcgcca
     3781 aggctagtaa tctataccag gatttgccaa atatttatca tcaaaatgat gttttagctt
     3841 tgtcatattt gttgagatat cggtaaaatg catgagttca ttaggaaaat ctctaccggt
     3901 aacacagata cttgtttttg ggtggtgttc ttctaaaagt tcgcgcagat gctgcacagt
     3961 aagtaaacca tcatggacag cattgtttat ttcatcacat ataacaaggt cgtaatcgcc
     4021 actggttgct gcttgaagag ctgctgtata tgtttttagc gctatagctt tatgttcgtt
     4081 ttccgtgaca tttttagcac ttagttcacc tgcattgtaa aaaccagccc cacctttcat
     4141 aaataataaa ttgtctccgt atataggtgt aatgttttct atgaacgtat gttcattaac
     4201 gtgccagtgc ttaataaatt ggatgtaggc aaccttccaa ttcgcaccaa gagcacgcgc
     4261 cattaagcca atgctcgcac ttgttttacc cttgccttcg ccagtgtata ccacgacgac
     4321 actatctttg cttttaaaat cttcaaaact catggtagtt atagcatacc atttttggta
     4381 ctaaggcttc gttgtttgat cagagttata aataccccgt tgttttgcta tagctatgag
     4441 tactaaccta tagatatttg taagactatg ctgtttatct gctgtaccct gttcgtgagc
     4501 ggttatgact gcacgttgta aaatacgagt aattgggtat aaagcgataa tgtgttcgcc
     4561 gagctcttga gtaataagct cattaaagcg cttttggtca aacataccgg ttgtttcgtc
     4621 tataaagagt tcataatcta ttaaaagttg ctcggctgcc ataatttgat cgtgacgttg
     4681 ctttgtgtct tctataacgt gttctttagg gatggcacga gttatttcta ataaacgtag
     4741 taaaaacgca cctatttttg gtgcactttc tactgtgtaa tacgtatcta aaaagcgagt
     4801 aatgtcttgc gacgtcggcc cgtctagtga ggtgcttatg agttcagcag agtaatgtgc
     4861 tggtggtgct ggctcaatcg tttcagtgct atcccgaggc atgggtatgt gggctctatg
     4921 gcgattatta tcgtattcta agagcagttc tgcgggtggt acaaatgttc catagattga
     4981 cttagccatg ccttgtcctc gcatatctct acgtacacta taactatata ctttttacaa
     5041 caaaatcgct acaggtagcc acccctatta ggtattgctt ttttttgata ttctttttat
     5101 tgtaagggta tatataaatg taaggagggt tctcttatga ttaagcaacg acatgttgct
     5161 gcgctgttgg tggagttttt tggcacactg acactcgcac tcgttgtact gagtgtaagc
     5221 ctgtataggt ttccattctt tactgcactt gctgcaggtg tgacggtggc tgtattcgtc
     5281 agcgcatttg caaaaatttc aggtgggcat gtaaatccag cggttaccgt tggtatgttt
     5341 gcaattcgtc aaatcactgc gctaagagca gttggctata ttgctgctca aatgctcgca
     5401 ggttttgctg catggcaact gtatgagtat ttaactgaaa gagcactaac aaatgctacg
     5461 acagctaccg tagaatggaa cgtagcagca gccgaagcac ttggtgcctt tatctttggc
     5521 ttggcactta ccggtgctgt agctcaaaaa cttaaagggt atcaattagc cttcactgct
     5581 ggagcaggcc tctttattgg tttaaccgta tctggtttag catcaaattc gttactaaac
     5641 cctgctgttg cagttgcagt acgaagtttt gatccagtta cctatgtagg tgcaccagtt
     5701 gtgggctatc tagttgctgc tgcgttggta gcgtacgttg ttgtaccaat gtttggcaaa
     5761 aaagaagtcg aaaagacgac tgctgtggct gccgcaaagc caagtgtcgc aaaaacagct
     5821 actaaaaaag ctcctgcaaa aaagcctgca gctaagaaaa aagctcctgc aaaaaagaag
     5881 aaataatcac aaataagctt taacgtatat taataacgtg gccatcaata cagtgccacg
     5941 ttatttttat ttgtttgcta taagccaatc ttcaaattcg gtaatgcctt tggcatcttc
     6001 actaaacaca ctaatatctg gaaaatctaa atttacgata catcgccata cacttgcaat
     6061 gattttccgc aacctggtaa gttcttgttc atctgtgatg tcaagcgaca agctatcgag
     6121 cgagccttgt tggtttggtt ctacaaacac tagttcacct ttagtagcgc gaataccctg
     6181 ttcgccccac gttcgtgatc catccacaag cagtttataa aagtataatt gttgtttata
     6241 gcgatgtaat ttttgtttag cgtagttcgt ttgaccttgc gcattccagg tcgtgctact
     6301 tgtgccggtt ttataatctg taacagttgc cgaatgtttg gtgacggtaa gcacgtctat
     6361 cttgcctgtt aataaagcgt cgccaattcg tacaccctgc ctacgaaaat cttgttctgg
     6421 taattgtttg ggggtaaact catgtgagcg ttgagcgtat aacgcattca gcgcattgat
     6481 ggcttgggtg tgatatttat cgtactcgtt tggtgctaaa ccctttcttt gtaaactaga
     6541 tgccataaac gattcgcttt tcgctaacgc aggtaatgag ccatgtttta ctacatagct
     6601 atgcatatat tcgagtgtac tatgcatggc gctaccaaaa ctagcgctcg ggctcattgc
     6661 agatggaaat tgaaggaaat tgtgtaaaaa gaacttatga ggcccaccgt ttggcacatc
     6721 caaaagatta tttaaatgag tagcgcttaa tgtataacgg ctaatacggt cttgtaatag
     6781 atcttttaaa gtagcttgag gcacagatag atgtttatcg tgccagtgac gttctgcgcg
     6841 aaataatatg tcatgtgggg tagaaggcat agccataggt tctacaggtg attgtatgat
     6901 tgcttggact gtcttttctt ctagtggggc atacgggatc atttctttgc cggtatcact
     6961 tgtcataaaa ctactcataa ataaatgacg cttagcacgt gtcatagcga cataaaacaa
     7021 tcgcaaatta tcatctgggt taggactatg agcaatactt agtaggttgg ggcttaaact
     7081 aatacctcca cccctcccct tactgttcca tatagattgg gtagcagaca gaatatatac
     7141 ggtatcaaat tcaagtcctt tgcttttgtg ggcagataat aagttaaccg gatccttacc
     7201 tgtagcgtgc atcattttca ctggtataga aacgtgtgct tttttggcta aatcgcaaaa
     7261 atgtatataa tcttgcaagc tatgagtgat atcaggtttg tacgatctca ggctcgagcg
     7321 tacggcgcta agtgcagcaa gtagttctaa gtacgcctgc ggcttgtttt gtaagtttgt
     7381 atcactgaaa tagtacgact taaagggcga tgcaattgtt ggagatgatg ctgtttccac
     7441 tg
//
LOCUS       DAOPLS010000016         7433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_10607_length_7433_cov_4.007997, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000016 DAOPLS010000000
VERSION     DAOPLS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10607_length_7433_cov_4.007997"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKV52_00750"
     CDS             <1..807
                     /locus_tag="PKV52_00750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPD98774.1"
                     /translation="KEAGDHYEVQVNGTNQEIVEYKLAGRTATVADGQDVTIGDVLAA
                     HEDGSDPIVASYSGTVAIKKTKVVLAPNTTSSMRYDIPGIKQLTVTDGAEVVAGSRLT
                     TGSINLQDLLRLRGPEATQRYIMNEINKIFAAQGQNIADKHLEIVIRQMFSKVVIDDS
                     GDSEFVIGDVVSKALVLEANQILAAEGKQLAEYSQMLLGITKVSTWADSFLSAASFQD
                     TTRVLISAATSGREDRLYGLKENVILGRKIPVGTGYKDIVLDDEPVEIVA"
     gene            831..1244
                     /gene="rpsL"
                     /locus_tag="PKV52_00755"
     CDS             831..1244
                     /gene="rpsL"
                     /locus_tag="PKV52_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPD98775.1"
                     /translation="MPTINQLVRKSRKSTGTKSKSPALHRVNNSLKNKTYEKSAPFKR
                     GVCVKVTTKTPKKPNSALRKVARVRLNNGHEVWAYIGGEGHNLQEHAVVLIRGGRVPD
                     LPGVRYHVVRRSLDLQGVANRKRGRSKYGAKKDAK"
     gene            1394..1873
                     /gene="rpsG"
                     /locus_tag="PKV52_00760"
     CDS             1394..1873
                     /gene="rpsG"
                     /locus_tag="PKV52_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPD98776.1"
                     /translation="MPRKKTKSLVRDIAPDRKYNSERVQRLINRIMLDGKKQVAERIV
                     YDAMDIAAKRLKQDNQLEVLNKALDNVRPSVETRSRRVGGANYQIPFEVHGQRQSHLT
                     LMWFVAAARGRKGMPMAQRVAAEIVDAYNNTGTAVKKKEDTHKMAESNRAFAHFARG"
     gene            2052..4139
                     /gene="fusA"
                     /locus_tag="PKV52_00765"
     CDS             2052..4139
                     /gene="fusA"
                     /locus_tag="PKV52_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPD98777.1"
                     /translation="MATKKTPMAKMRNIGIIAHIDAGKTTTTEGILYRTGLKHKIGAV
                     HEGGTTTDWMEQERERGITIVSAAVTCYWKEHQINIIDTPGHIDFTVEVERSLRVLDG
                     AVVVFDGKMGVESQSETVWRQADKYSVPRICFINKINQTGGDFYKSLESIHNRLSKRA
                     FPIHLPIGFEQKINGLVDLVTLKAYTYKAFTDHELVESDIPADMQEKVAKYRSLLIEN
                     AVAEDEPMLEKYLEVGEEGLTEADIKQSIRTATLSGNFFAVTGGDGRGVIVEKVLDLV
                     TDFLPSPEERGSTWGTNPKNDEEIERKPLESEPFSALAFKIATDPFVGKLAYFRVYSG
                     KVESGSYVFNSSTGNKERIGRIVRMQADKREDVSVVYAGDIAAIVGLKGTTTGNTLCD
                     PAHPIVLESITFPEPPVSIAIEPATKADQEKMSLALQRLAEEDPTFRIKVDHETGQTI
                     ISGMGELHLEIIVDRMKREFSVEANIGAPQVAYRETIRKDGVEIQGKFIRQSGGRGQY
                     GDVWLRLSQNEAGKGFEFINSIKGGVVPSEYIPAVKAGIIEAMANGVLAGYPVVDLKA
                     ELYYGSYHDVDSSEMAFKIAGSMALQDGVKLAKPVLLEPIMKVVVVVPEEYMGDVIGD
                     LNAKRGRIESMEDLQAGIKEITSFVPLGEMFGYTTNLRSMTKGRASSSMELDHYADVP
                     NHVTEAIIAKAGK"
     gene            4159..4383
                     /locus_tag="PKV52_00770"
     CDS             4159..4383
                     /locus_tag="PKV52_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98778.1"
                     /translation="MDSKFTFIDEYDGPHTRLLALDVKAMDSWSERIGYATGVLAAKT
                     VLEAMNLQGKALRSLGAVAHRAGVPRGKSS"
     gene            4550..5731
                     /gene="tuf"
                     /locus_tag="PKV52_00775"
     CDS             4550..5731
                     /gene="tuf"
                     /locus_tag="PKV52_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPD98779.1"
                     /translation="MADFDRSKPHVNVGTMGHVDHGKTSLTAAITMTLAKRLPSDVNK
                     AVNYDQIDNAPEEKQRGITIATSHQEYESEKRHYAHVDMPGHADYVKNMITGAAQIDG
                     AILVVSAADGPMPQTREHVLLAKQVGVPKILVFLNKMDLADPELVELVEMDVRELLAK
                     YDFDEDAPIIKGSATKAIEGDAANEDAVMELIAAMDDYIPEPARDLDKPFMMAIEDVF
                     SIKGRGTVATGRIEQGIVKVNDEVEIVGVKPTQKSVVTGIEMFKKSLNQGQAGDNAGI
                     LLRGIERDDIERGQVLCKPGSITPHTEFDAEVYILTKEEGGRHTPFFKGYKPQFYFRT
                     TDVTGEVELPADKEMVMPGDTVTFKVTLLAPIAMEQGLRFAIREGGRTVGAGVVTKIN
                     K"
     gene            5911..6225
                     /gene="rpsJ"
                     /locus_tag="PKV52_00780"
     CDS             5911..6225
                     /gene="rpsJ"
                     /locus_tag="PKV52_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPD98780.1"
                     /translation="MAETQQRIRIRLKAYDHKVIDQSAKQIVETALRTGATIAGPIPL
                     PTRKTVYTVVKSPHVYKKGREQFEMRVHKRLIDITNPTPKTIDSLMNLSLPAGCDAEI
                     KM"
     gene            6346..7392
                     /locus_tag="PKV52_00785"
     CDS             6346..7392
                     /locus_tag="PKV52_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98781.1"
                     /translation="MFKRLKKPAEKPEQPLYDRQPGLSRRDLLKRLAYTAGGVAVMGP
                     YVQRLDSIATHERWPDDDFMIIPSEGSETLANGGEEWLVFGGFGQKYSVNAAQELFSA
                     IGQCQVVASIMYPNQGFTIEELGACIEEYVQARHMSTLNIVGVSMGLPTALMALTTLR
                     AGAKPTSLPKINYLAAYSSPADVRDAIDGDLAQLVSDLGKYTDHMPVIPPKFIYSLLD
                     GDGDVQRFLNIQDNAQWVQHVRDSIKQTMNDCSPELALSQIKIISAFNVEEQWQSLRN
                     IVSPGNTKFIYCAPSGGDDTVDNTNAANKYRDALTRLSVPTTVLDTGLSGHANTVASA
                     QVLGRLVAHQTTIH"
BASE COUNT         2340 a         1473 c         1741 g         1879 t
ORIGIN      
        1 aaagaagcag gtgatcacta cgaggtgcaa gtgaatggta cgaatcaaga aattgtcgag
       61 tataaactgg ctggacgtac tgcaacagtt gcagacggcc aagatgtcac aataggtgac
      121 gtattagctg ctcacgaaga tggcagtgac ccaattgttg ccagttactc gggtacggta
      181 gcgattaaaa aaaccaaagt agtgttagct ccgaacacaa cgtcttcaat gcgttatgat
      241 atcccaggca tcaagcaatt aactgttacc gacggtgcag aagtagtggc gggaagtcgc
      301 ttaacaacag gttcaatcaa tttacaagac ctactgcgac ttcgcgggcc agaggcaaca
      361 caacgctata tcatgaacga aatcaacaag atttttgcgg cacaagggca aaatattgcc
      421 gacaagcact tagaaattgt gattcggcaa atgtttagca aggtagtgat agacgattca
      481 ggcgacagcg agttcgttat aggtgacgtc gtcagcaaag cgttggtact cgaagccaac
      541 caaatactgg cagcagaagg taagcaattg gccgaatata gtcaaatgct cctcggtatc
      601 accaaggtat ctacctgggc agatagcttt ttgagtgctg cgagcttcca agacaccacc
      661 cgtgtgttaa tatctgcagc aaccagtggt cgcgaagatc ggctgtatgg tttgaaagaa
      721 aacgtaatct taggtcgaaa gattccagta ggtacaggct ataaagatat tgtgctcgat
      781 gacgagccag ttgaaatagt agcgtaattc tgttaaagta ggaatttgta atgccaacaa
      841 ttaatcaatt agtaagaaaa tcacgcaagt ctacagggac gaaaagtaaa tctcccgctt
      901 tgcaccgcgt caataatagc ttaaagaata agacgtatga aaaatcggca ccctttaagc
      961 gtggcgtatg tgttaaggta accaccaaaa caccaaaaaa acctaactcg gcactccgaa
     1021 aagtagcccg tgtacgttta aataatggtc acgaagtatg ggcctacatt ggtggtgaag
     1081 gacataattt acaagaacac gctgttgtcc ttattcgtgg cggtcgtgtg ccggatctac
     1141 ccggtgtacg ctaccatgtt gttcgtcgca gccttgacct tcaaggggta gcgaaccgta
     1201 agcgtggtcg tagtaaatac ggcgcaaaaa aagacgctaa atagtattta ttgcagctga
     1261 tagtggagta gctacgagct actccataca tctgcaataa agcaggagta attttaataa
     1321 taagtggctt tcgtaacccc ccagtttgca catatgtaaa cgggagacgg aagaggaaag
     1381 gaacggttct taaatgccac gaaaaaagac gaagagtttg gtgcgagata ttgctccaga
     1441 cagaaaatat aattcagagc gtgtgcaacg ccttatcaat cgaattatgc tcgatggtaa
     1501 gaagcaagtt gcagaacgca tcgtatatga cgcaatggat attgcggcaa aacgtttaaa
     1561 gcaagacaat cagctagaag tactaaataa agcattagat aatgttagac caagtgtaga
     1621 aactcgctca cgccgcgttg gtggggcaaa ctaccagata ccgtttgaag tacacggcca
     1681 gcgtcagagc catttaacgc ttatgtggtt tgttgcagct gccagaggac gcaaaggaat
     1741 gccaatggct cagcgagttg cagctgaaat tgtagatgca tataataata caggcacagc
     1801 ggtgaagaaa aaagaagata ctcataaaat ggcagagtct aaccgagcat ttgctcactt
     1861 tgcccgtggg taagtaacgt acaggtaaac gtcttgtaaa aaggtgactc attaaaatac
     1921 atgacgatgt catcataaag ctcactttag aggcgcaaga tagaatatct agatacgatg
     1981 caaatagaac accaagtaat gtagaaatag aagaagtaga ctaatcataa tagaacgaag
     2041 aaggaataat aatggcaacc aaaaaaactc caatggctaa aatgcgtaac atcggtatta
     2101 ttgcgcacat tgatgctgga aagacaacca ccacagaagg catcctgtat cgtacagggc
     2161 ttaaacataa aattggtgca gtccacgaag gtggcactac taccgactgg atggaacaag
     2221 aacgtgagcg gggcataaca attgtatctg cagccgtgac gtgctactgg aaagaacatc
     2281 agattaatat cattgatacg ccgggtcata ttgattttac ggtagaagtt gagcgcagtt
     2341 tacgcgtgtt agatggcgcc gttgttgtat ttgacggcaa aatgggcgta gaaagtcagt
     2401 ctgaaaccgt atggcgtcaa gccgacaaat acagcgtgcc tcgtatttgt tttatcaata
     2461 aaatcaatca aacaggtggt gatttctata agagcctaga gtcaattcat aaccgtctta
     2521 gcaagcgtgc atttccgatc catctcccta ttggttttga acagaaaatc aatggccttg
     2581 tagacctcgt aaccctaaaa gcatatacct ataaagcatt taccgatcac gaactcgttg
     2641 agtcagacat accagctgac atgcaagaaa aagttgctaa atatcgcagc ttattaattg
     2701 aaaacgctgt cgctgaagat gaaccgatgc tagaaaaata cctcgaagtc ggcgaagaag
     2761 gtttaacaga agccgatatt aaacagtcta ttcgcacagc gacactaagt ggaaactttt
     2821 ttgccgttac tggcggcgat ggccgtggtg tcattgtaga aaaagtgcta gatctcgtta
     2881 ctgattttct accaagccca gaagaacgtg gttcaacgtg gggcacaaat ccaaaaaatg
     2941 atgaagaaat tgaacgtaaa ccattagaaa gcgaaccttt ttctgcattg gccttcaaaa
     3001 ttgcgacaga tccttttgta ggaaaactcg catactttag ggtgtattca ggcaaggtag
     3061 agtcaggtag ttatgttttt aatagttcta caggtaacaa agaaagaatt ggccgtattg
     3121 tccgtatgca agcagataag cgcgaagacg tctctgttgt atatgcaggt gatattgccg
     3181 ctattgttgg cttaaaaggt accacgactg gtaatacatt atgtgaccca gcacatccta
     3241 tagtgctgga aagcattacc ttcccagagc ctcctgtcag tatcgctatt gaaccagcta
     3301 ccaaggcaga ccaagaaaaa atgagtcttg ccttgcaacg actcgctgaa gaagatccaa
     3361 cttttagaat taaagtagac cacgaaaccg gccaaacaat tataagtggt atgggcgaac
     3421 tgcatttaga aatcattgta gatcgcatga aacgagaatt cagtgtagaa gcgaatattg
     3481 gtgcgccgca agtagcctat cgtgaaacaa tacgtaaaga cggggttgaa atacagggta
     3541 aattcattcg tcaaagtggc ggacgtgggc agtacggtga cgtatggtta cgtctcagcc
     3601 agaacgaagc tggcaaaggc tttgagttca ttaatagtat caagggtggg gttgtgccaa
     3661 gcgaatacat ccctgctgtt aaggccggta ttatcgaagc tatggctaat ggagttcttg
     3721 cagggtatcc tgttgtagac ctaaaggcag aactctacta tgggagctat cacgatgttg
     3781 actcaagtga gatggccttt aagattgcag gttcaatggc gctgcaagac ggggtaaagc
     3841 ttgcgaaacc agtgttgctc gagcctatta tgaaagttgt tgtggtggtc ccagaagaat
     3901 atatgggtga tgttattggt gatcttaacg caaagcgtgg ccgaatagaa tctatggagg
     3961 atttgcaagc tggaataaaa gaaataactt catttgtccc ccttggggaa atgtttggtt
     4021 acacgacaaa tctaaggtct atgacaaaag gtcgtgctag ctcaagcatg gaacttgacc
     4081 attatgctga tgtaccaaac cacgtcactg aagctataat tgcaaaagca ggcaagtaat
     4141 caacttgtta attgccagat ggatagcaaa ttcacattca ttgatgaata tgatggtccg
     4201 cacacccggc tgttagcgtt agatgttaaa gcaatggact catggtcaga gcgtatcggc
     4261 tacgctacgg gtgttttggc tgccaaaaca gttctagaag cgatgaacct tcaaggcaaa
     4321 gcgttacgtt cgttgggtgc agtagctcat agagcaggcg ttccacgggg caaatctagc
     4381 taaaatatta cgtaggcgta agattgcgta aaagcacaat cataaaataa aaaaacacct
     4441 ttacaacgag gctgtgtttt cggtacacta gtagggttat tgcgtgaggc gttgcctctt
     4501 tatggcgcaa aaaatgtata taaattaatc actcttaaag gagaaatgaa tggcagattt
     4561 tgacagaagc aagccacacg tgaatgttgg taccatgggt cacgttgacc acggaaaaac
     4621 ttcactaacc gctgctatca ctatgacact cgcaaagcgc ttgccaagcg atgttaataa
     4681 agcagtaaat tatgatcaaa ttgataacgc accagaagag aagcagcgtg gtattaccat
     4741 tgctacctct caccaagaat atgagagcga aaagcgtcac tatgctcacg tagacatgcc
     4801 tggtcacgcc gactatgtta aaaacatgat tactggtgca gctcaaattg acggagctat
     4861 tttggtagtg tctgcggctg atggccctat gcctcaaacc cgtgaacacg ttcttcttgc
     4921 taagcaagtg ggagtgccta agattctcgt gtttctcaat aaaatggacc ttgcagatcc
     4981 agaactcgta gagcttgtag aaatggatgt tcgcgagcta ctcgctaaat atgattttga
     5041 tgaagatgct ccgattatca aaggttcagc aaccaaggct atcgaaggcg atgcagcgaa
     5101 cgaagatgcc gtcatggaac tgattgcagc tatggatgat tacattccag agccagctcg
     5161 tgaccttgac aagccgttca tgatggctat agaagacgtc ttttcaatca aaggtcgtgg
     5221 taccgtggct accggccgta tcgaacaagg tattgtaaaa gttaatgatg aagtagaaat
     5281 tgttggtgtt aaaccaactc aaaagagtgt tgtaactggt atagagatgt ttaagaagag
     5341 ccttaatcaa ggccaagctg gtgataacgc tggaattttg ctccgcggta tcgaacgtga
     5401 tgacatcgag cgcggtcaag tgctgtgtaa accaggttct attacacctc acaccgaatt
     5461 cgatgcagag gtttatattc ttacaaaaga agaaggtggc cgacacacgc cgttctttaa
     5521 aggctacaag ccacagtttt actttagaac aacggacgta acaggcgaag tagaattgcc
     5581 agctgacaaa gaaatggtca tgccaggtga tacagtaacc tttaaagtta cgcttcttgc
     5641 acctattgca atggagcagg gtttacgctt tgcaatccgc gaaggtggtc gcacggttgg
     5701 tgctggtgtt gtaaccaaaa tcaacaaata gtaatttgta catatcttag tgcaacaaga
     5761 aaagcctcgc aagaggcttt ttttgataat acaagcaatt gtgttatgat atattagtac
     5821 attaattgta gtgactaaaa gtcttagtgc gtgctctttc atagcttgcc aaagatgtta
     5881 cactacgcat agaaagacag ggtattattc atggcagaaa ctcaacagcg tattcgcata
     5941 cgactcaagg cgtatgatca taaagtcatt gatcaatccg ctaagcaaat agtagaaaca
     6001 gcacttcgta ccggtgctac catagccggc cccataccat tgccgacacg aaagacggtg
     6061 tatactgtag tgaaaagtcc tcatgtttac aaaaaagggc gcgaacaatt tgagatgcgc
     6121 gttcataaac gacttatcga tattaccaac ccgacaccga aaaccattga ttcattaatg
     6181 aatctaagct taccagcagg gtgtgacgcc gaaattaaaa tgtaatttct ctgagtatta
     6241 agatacaaat gccgtagaca aaagtttacg gcatttgtat tacaaaatac attagaaatg
     6301 cttatgctac caaggttgtt ttcaacagtg tatacttttt tatgtatgtt taaaagatta
     6361 aaaaagccag ccgaaaaacc tgagcagcct ttatatgatc gtcagccagg tttgtcacga
     6421 cgcgatctcc tcaaacgttt agcttacact gctggaggtg tagcagtaat gggtccctat
     6481 gtacaacgat tagatagcat tgctactcac gaacgatggc ccgacgacga ctttatgatt
     6541 attccgtcag aaggttctga gactttagca aatggtggtg aagagtggct tgtatttgga
     6601 ggctttggtc aaaagtatag tgtgaatgct gcccaagaat tattttccgc tattggtcag
     6661 tgtcaggtcg tggcgtctat tatgtatcct aaccaaggat ttaccataga agaactgggt
     6721 gcttgcatag aagaatatgt acaagcacgc cacatgagta cgttgaatat cgttggtgtt
     6781 agcatggggc taccaaccgc ccttatggcg ctaactactt tacgagctgg tgccaagcca
     6841 acatcattgc ctaagattaa ttatcttgct gcatattcat cgcctgcaga cgtaagagac
     6901 gctattgatg gtgatttggc acagcttgta tcagacttag gtaaatatac tgatcacatg
     6961 cctgttatac cacccaaatt tatctatagt ttactagacg gcgacggtga cgttcagaga
     7021 tttttaaata ttcaggacaa tgcgcagtgg gtgcagcacg tacgcgatag tataaagcaa
     7081 acaatgaatg attgttcacc agagttagct ttatcacaga ttaaaattat cagtgctttt
     7141 aatgtagaag aacaatggca gtcgctccgc aatattgtgt cacctgggaa cacgaaattt
     7201 atatattgtg caccttcagg tggagatgac actgtagata atacaaacgc cgccaataaa
     7261 tatcgtgatg cccttacacg tctatcggta cctaccacag tattagacac tgggttgagt
     7321 ggtcatgcca acaccgttgc atctgcccaa gtattgggac gcctcgtcgc acatcaaaca
     7381 actatccatt gatgcttatc taaagcggtg tcacaagcta cgagccggtg act
//
LOCUS       DAOPLS010000017         7420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_10631_length_7420_cov_3.716768, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000017 DAOPLS010000000
VERSION     DAOPLS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10631_length_7420_cov_3.716768"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /gene="pilO"
                     /locus_tag="PKV52_00790"
     CDS             <1..672
                     /gene="pilO"
                     /locus_tag="PKV52_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPD98782.1"
                     /translation="TTVIVAAAAAAAIVVFSLFASKALIDKIGYQNKVISKRNVANKQ
                     LKENIDAKNQLVVSFAAFDESPESVLGTSEKNSKIVLDALPSKYDFPALTTSIDGIIT
                     GAGMSVESITGADQEISAEQSSVNPTPIAIPFEFGAIGTYEAAQKLLTDLQRSIRPFK
                     IHSVDFTASEGGVVSVKVTGETFYQPAKDLGIQYKKVDKNGKFIGGNEATPPATDTNT
                     TGSSN"
     gene            673..903
                     /locus_tag="PKV52_00795"
     CDS             673..903
                     /locus_tag="PKV52_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98783.1"
                     /translation="MKRKDIMVIVGIAIVSAIFSYVISNALFGTQTDQSKLLEAPEVQ
                     PISAEFPTPDERFFNPQSLNPTKNITIGDYSQ"
     gene            917..2689
                     /locus_tag="PKV52_00800"
     CDS             917..2689
                     /locus_tag="PKV52_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPD98784.1"
                     /translation="MPVLLEDTQKQVEQLLVKNGLIEKAQLDAVHAKAKEKGEPFFQL
                     LVTEEHVTGEELTKAIAQVTRVPYVNLSTARVNPKVLSLLPLEVAERYMAVPLGEMQN
                     RLVVAMLDASNVQAVDFLSNKIGRPLKVYMASQQGIEDVIKQYGVDVTKDVEKALEQK
                     KSDDAAGATKTKSIQTIVQDSPISKALAAIMDYAAKSRASDIHIEPLEKELKIRCRID
                     GVLREIMKLPKSTEPALISRIKILSNLKIDEHRIPQDGQFSIMAGGKAIDLRIAISPV
                     VWGEQCVIRLLDKSGTSLMLEDMGYSGRALRTIREGAKNPNGMILTSGPTGSGKSTSM
                     YALIQEIKSDEINIVTLEDPVEYKMEGINQIQVNSEVGLTFASGLRSILRQDPDVVMV
                     GEIRDAETASLAVQAALTGHLVLSTLHTNSAAGILPRLLDMGIEPFLIASTVRTVIGQ
                     RLVRRLHKPEPTESSPIEAKAITETIGSFLPTTEEAKPAVVEDLGYGDLPLANQNAYT
                     LYKGKDSPITPGGYKGRLGLYEVFSVTEEIQDLIIKHATSAQIQEMAQQQGMITMRQD
                     GYFKALSGRTSIAEVNRVAASDSA"
     gene            2709..3779
                     /locus_tag="PKV52_00805"
     CDS             2709..3779
                     /locus_tag="PKV52_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPD98785.1"
                     /translation="MQVAQPRIEVLLEEVIKKKASDLHLQVGLQPMLRVDGALKAVSG
                     TDVLTEETVESLVFALLDDDQKQILLKDKEFDFSFAFGDLGRFRVNAFHERGNLAAAL
                     RLIPSELLSVEQLGLPNIVNKFAEYPRGLVLVTGPTGSGKSTTLASMIHKINTERAEH
                     IVTIEDPIEFTHKSLKSVIVQREVHYDTFSFSAALRSSLRQDPDVVLIGEMRDLETIA
                     AAITIAETGHLVFATLHTNSAAQSIDRMIDVFPPHQQPQIRSQLSNILMAICSQRLIP
                     AIGGGRIAAAEILVATPAVRNIVREGKTHQLDAVIQTGAEFGMQSMDKTLVSLIHSGT
                     ITYEEARNYAVDLEELDRLMRG"
     gene            3784..4986
                     /locus_tag="PKV52_00810"
     CDS             3784..4986
                     /locus_tag="PKV52_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD98786.1"
                     /translation="MSTFTFTARNPQTGQKVTSTVQADNERAAAKAIEGQGLAVVEIK
                     VEKEGGFGKKVKAKDRILFARQLSTLINAGLPLVQSLRTVQRQTSSKPMKIIIAEVIG
                     QVESGKNFSDALAAHPKVFSTVFVSLVAAGEVSGTLDNSLERLAFQQEKDAEILGKIR
                     GALIYPLVVIGVMIAVIGFMLVGVLPQVKVLYDSLPGAELPLITKVLLWMSNMLVTYW
                     WVVIILIGFGVFFGSRWARTLGGKRYIDKFKMKAPLIGPLFMKMYMARFARTGTTLVA
                     SGVPLLQMLEITSKSVNNVYIEDSIDHAISQVKGGKSLADSLEGDPNFLELVPNMLRI
                     GEQSGQVESMLDKTAEYYEKEVDQQIKTINTIIEPVLMIVLGVVALIIVAAVLLPIYG
                     LANQGLGA"
     gene            5074..5520
                     /locus_tag="PKV52_00815"
     CDS             5074..5520
                     /locus_tag="PKV52_00815"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPD98787.1"
                     /translation="MFKKENKGFTIIEVLIVLAIAGLIMLIVFLAVPALQRNSRNTSI
                     KNDAQLFAGAVGDHRASYNGTTPTVTEGTGTITLSGGGTSTVTLNGSTPVAIPTDKPN
                     ADTMVPGTLYVASGRDCNYSQSARTVAIWFVTETSGRGEAVLCIEG"
     gene            5612..6481
                     /locus_tag="PKV52_00820"
     CDS             5612..6481
                     /locus_tag="PKV52_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPD98788.1"
                     /translation="MLKVYLFVLGLIWGSFINAWVWRLRQLLNDDGDPKKLSATKRKE
                     LSILHGRSMCPSCHHQLTARDLIPVLSWLLLKGKCRYCHAPISAQYPLVELITAVVFA
                     LSYIFWPISTAQYGWVLLAGWLVTLVFLLVLAIYDAKWYILPSKLIYLGIITYGASLL
                     FYAVLSGVTSTLVNAIAAGVLYLAGFFALFYVSYIVQQHKTGAKAWLGFGDVRLAFLL
                     GLIAGSPLNVFIAVFLASLVGLAVAIPGIVSKKLSVTSHIPFGPFLILGASLALFFGD
                     SLSSWYSTVLLGL"
     gene            6534..7268
                     /locus_tag="PKV52_00825"
     CDS             6534..7268
                     /locus_tag="PKV52_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPD98789.1"
                     /translation="MKLIKKQTGYTILETLIVLTVTGVLFTTTALLIQGQVEKTRYQD
                     SMRQIQQTVQQAIKDTENGYFPGATGNDTAGTVLAGKRIYFCTDAPDPNDDENSRQPC
                     TAGKSMIRVENIYADSGGSLDTDVELSNGKETYITYPGSINYKYHHKSNGTYPRESTG
                     FAVMFKNFSTTDYGSQTTSLYEAYIDDNNITTRSEDEWTKDRTPLYWNQGKRICFDGY
                     KKGSLIIGRNGSTNVEMNLDDPECIH"
BASE COUNT         2273 a         1529 c         1677 g         1941 t
ORIGIN      
        1 accacagtta ttgttgccgc tgcggcggct gcagccattg tcgtatttag cttatttgcc
       61 agtaaggctt taatagataa gataggctac caaaacaaag tgattagcaa aagaaatgtt
      121 gccaataaac agcttaaaga aaacatagac gcaaaaaatc agcttgttgt ctcgtttgcg
      181 gcgtttgatg aaagtccaga atctgtgctg ggcacatcag aaaaaaatag caaaattgtg
      241 ctcgatgcac taccaagtaa atacgacttt ccggccctta ccaccagtat agatggcatc
      301 attactggtg ctggtatgtc cgtagagagc attacaggag cagaccaaga aatttctgca
      361 gaacagagta gcgtgaaccc aacacctatt gctataccgt ttgaatttgg tgcaattggt
      421 acgtacgaag ctgcccaaaa attactcact gatctgcagc ggtctatccg accatttaaa
      481 attcattcgg ttgatttcac agcctctgag ggcggtgtag ttagtgtaaa agttacgggc
      541 gaaacgttct atcaaccagc taaagactta ggcattcagt acaaaaaagt agataaaaat
      601 ggtaaattta ttggtggtaa cgaagcaaca ccgccggcta cagacactaa cacaacaggg
      661 agtagtaact aaatgaaacg caaagatatt atggttattg taggtatcgc cattgtgagt
      721 gctatcttta gttatgtgat ttctaatgca ctgtttggca cacagacaga ccaaagcaaa
      781 ttgctagaag ccccagaagt acagccaata tctgctgaat ttcctacacc agacgaacga
      841 ttctttaacc cccaatcgct taacccaacc aagaatatta ctattggcga ctattcacag
      901 taaggagtac taaaaagtgc ccgtactact agaagatact caaaaacagg tagagcagct
      961 gttggttaaa aacggtctca tagaaaaagc ccagctagat gccgtgcacg ccaaagcgaa
     1021 agaaaaaggt gaaccgtttt ttcagttact agttaccgaa gaacacgtta ccggcgaaga
     1081 acttaccaaa gctattgccc aagtaacccg tgtaccgtac gtaaacctta gcactgcccg
     1141 cgtaaaccct aaagtgctgt ctttgttgcc gttagaggtt gcagagcgtt acatggccgt
     1201 accgcttggc gaaatgcaaa ataggctggt tgtagctatg ttagatgcca gcaacgtgca
     1261 ggctgtagac tttttatcta acaaaattgg tcgcccgctt aaagtgtaca tggcgagcca
     1321 gcagggtata gaagacgtta ttaaacagta tggtgttgat gttacgaagg atgtagaaaa
     1381 agccctcgaa caaaaaaagt cagatgatgc cgccggtgct accaagacaa agagtattca
     1441 aaccattgtt caagattcgc ctattagtaa agccttggca gcaattatgg attatgcagc
     1501 caaaagtcgc gccagtgata ttcatataga accactagaa aaagaactca aaattcgttg
     1561 ccgtatagat ggcgtgttac gcgaaattat gaagttacca aaatctaccg aaccagcact
     1621 tatatcgcgc attaaaattt tatctaacct taaaatagac gaacatcgca taccacaaga
     1681 cggccagttt agtattatgg ccggtggcaa agctattgat ctgcgtattg caatctcgcc
     1741 cgtggtatgg ggtgaacagt gtgttattcg tttgctagat aaatctggca ctagccttat
     1801 gctagaagac atgggctatt ctggtagagc cctccgtacg attcgtgaag gtgctaaaaa
     1861 tcctaatggg atgattttaa catctgggcc tactggttct ggtaaatcaa ccagtatgta
     1921 tgcgcttatc caagaaatta aaagcgatga aattaacatt gttactctag aagatcctgt
     1981 tgaatataaa atggaaggca ttaaccaaat acaggttaat agtgaggtag ggctaacctt
     2041 tgccagtggc ctcaggtcta ttttgcgcca agacccagac gtagtaatgg ttggggaaat
     2101 tcgtgatgca gaaacggcca gcctagcagt gcaagctgca cttacaggtc acttggtgct
     2161 gtctacgctg cacacaaata gtgcagctgg tattttgccc cgtttgctcg atatgggcat
     2221 agaaccgttt cttattgcca gtacggtgcg gacagtcatt ggccagcgtt tagtacgccg
     2281 tttgcataaa ccagaaccaa ctgaatctag ccccatagaa gcaaaagcca taacagagac
     2341 aatcggctct tttttgccaa ccacagaaga agccaaacca gccgttgtag aagatttagg
     2401 ctacggggac ttgccactgg ctaaccaaaa cgcttatact ttgtataagg gtaaagattc
     2461 accaatcaca cccggtgggt ataaagggcg cctaggactc tacgaagtct ttagcgtcac
     2521 cgaagaaata caagatttaa ttatcaaaca tgctaccagt gcccaaatac aagaaatggc
     2581 acaacagcaa gggatgataa cgatgcggca agatggctat tttaaggcat taagtggccg
     2641 aacttctata gcagaagtca accgcgttgc agcatcagat agcgcataac aaataataag
     2701 gtggaaccat gcaagtagca caaccaagaa tagaagtact cctagaagaa gtaatcaaga
     2761 aaaaggccag tgacctccac ttacaagttg gtttgcaacc catgttgcgt gtagatggtg
     2821 ctttaaaagc agtaagtgga acagatgttc ttacagaaga aaccgtagaa agcttagtgt
     2881 ttgcattgct agatgacgat caaaagcaga ttttgctaaa agacaaagaa tttgacttta
     2941 gctttgcgtt tggtgattta gggcggttcc gcgtaaatgc ctttcacgaa cgtggtaact
     3001 tggcagcagc gctgcgttta ataccaagcg agcttttatc tgttgagcaa cttggcttgc
     3061 caaacattgt gaataaattt gccgaatacc cgcgtggttt agtattggtt actgggccaa
     3121 ctggttctgg aaaatctact actttggcgt caatgattca taaaattaac accgaacgtg
     3181 ctgaacacat cgttaccata gaagatccaa tagaatttac ccataaaagc cttaaaagcg
     3241 tgatagtaca gcgcgaagtt cactacgata cgtttagttt tagcgcagcc ttgcgtagta
     3301 gcttgcgcca agacccagat gtagtactta ttggcgaaat gcgagatcta gaaacgattg
     3361 ccgctgctat tacaattgca gaaacaggcc acttggtatt tgcgacgcta cacacaaaca
     3421 gtgccgccca aagtatagac cgtatgatag acgtgttccc gccacaccag cagccacaaa
     3481 ttcgctcaca gcttagtaat attttaatgg ctatctgttc gcagcggctt atcccagcta
     3541 taggtggtgg ccgtattgca gctgcagaaa tactagtggc aactccggcc gtacgtaaca
     3601 ttgtgcgcga aggcaagacg catcagctag acgctgttat acaaactggc gcagaatttg
     3661 gtatgcaaag catggataaa acactagtga gccttattca cagcggaaca attacgtacg
     3721 aagaagcacg taattatgcc gtagacctag aagagctaga taggctcatg agaggctaaa
     3781 gctatgtcaa catttacctt taccgctcgt aatccacaaa ccggccaaaa agttacctct
     3841 actgttcagg cagataacga acgtgctgcc gccaaagcca tagaaggcca aggccttgca
     3901 gtagtagaaa tcaaagtaga aaaagaaggc ggctttggta aaaaggttaa agcaaaagat
     3961 cgtatactgt ttgcgcgcca gttaagcacg ctcattaacg ctggtttacc actggtgcaa
     4021 tcactccgta ccgtgcagcg ccaaaccagc agtaaaccca tgaagattat tattgcagaa
     4081 gttattgggc aggtagaatc tggtaaaaac ttttcagacg ccctagcagc ccaccccaaa
     4141 gtgtttagta cggtgtttgt gagccttgtg gcagcaggtg aagtgtctgg tacactagat
     4201 aactcgcttg aacggcttgc ctttcagcaa gaaaaagacg ccgaaattct tggtaaaata
     4261 cgtggcgcgc taatatatcc actcgttgtt ataggtgtaa tgatagcagt cattggcttt
     4321 atgcttgtag gtgtattacc gcaagtaaaa gttttatacg atagcctgcc tggtgcagaa
     4381 ctaccactca ttaccaaagt actactatgg atgtctaaca tgctggtgac gtactggtgg
     4441 gtcgtcatta tcttaatagg gtttggtgta ttttttggta gcagatgggc ccgcacactt
     4501 ggcggtaaac gctacataga taaatttaaa atgaaggcac cacttattgg cccactgttt
     4561 atgaagatgt acatggcgcg ttttgcccgt actggtacca cactggttgc cagcggtgta
     4621 ccattgctac agatgctaga aattactagt aaatcggtaa ataatgtata catagaagat
     4681 tctatagacc acgctattag ccaagtaaaa ggtggtaaaa gcctagcaga ttcactcgag
     4741 ggcgacccca acttcttaga gctagtgcca aatatgttgc gtattggcga acaatctggc
     4801 caggtagaat ctatgctaga taaaactgcc gaatattacg aaaaagaagt agaccaacaa
     4861 attaagacca tcaacaccat catagaacca gtacttatga ttgtgctggg tgtcgtggcc
     4921 cttattattg ttgctgcggt actactgcct atttacggct tggctaatca ggggctaggc
     4981 gcttaaaaaa cgcttgcatt taattggtaa tacagtatta ttaaggtatt ataagtaaac
     5041 ataatcatgt tcaaaagaag gggcaccaaa catatgttta aaaaagaaaa taaagggttt
     5101 acaatcatcg aggttttaat tgtgctagcc attgcaggct taataatgct catagtattc
     5161 ttagcggttc cggctttgca gcgtaatagc cgtaatacat caattaaaaa tgacgcgcag
     5221 ctttttgctg gtgccgttgg agaccatagg gcaagctata acggaactac cccaacggtg
     5281 accgaaggga caggtacaat tacacttagc gggggcggaa cttcaaccgt aacgctaaat
     5341 ggatcaacgc ctgtagcgat acctactgat aaaccaaatg ctgacactat ggttcctggc
     5401 acactgtacg tagctagtgg tagagattgt aactatagtc aaagcgcaag aactgttgcc
     5461 atatggtttg tcacagagac atcgggcagg ggcgaagcag ttctgtgtat tgaaggctaa
     5521 agggtatagt gcataaataa aagaaacggc tatagcagcc gtttcttttc ttttactcaa
     5581 tatctgtgag atacttttta ggatgagcat tttgttaaaa gtctatttat ttgtacttgg
     5641 gcttatatgg ggcagcttta ttaacgcgtg ggtgtggcgc ttgcgccaac tgctcaatga
     5701 cgatggcgac ccaaaaaagc tctctgccac aaaacgtaaa gaactgtcta tactgcatgg
     5761 ccgtagcatg tgccccagtt gccaccacca actgactgcc agagacttga tacctgtgct
     5821 tagctggctg ctactaaaag gcaagtgccg ctactgccat gcgccaatta gtgcccagta
     5881 tccgcttgta gagcttataa cggcagtagt atttgctttg tcttatatct tttggcccat
     5941 tagtacagcg cagtatggct gggtactgct tgctggctgg cttgttaccc tagtattttt
     6001 gctcgtgctg gcgatatacg atgcaaaatg gtacattttg ccatctaaac ttatttacct
     6061 aggtattatt acgtacggcg caagtttgct gttttatgca gtgcttagcg gtgtaactag
     6121 cacactggtt aatgcaattg ctgcaggtgt gctttattta gctggttttt ttgcgctttt
     6181 ttatgtgtca tatattgtgc aacaacataa aaccggtgca aaagcgtggc ttggctttgg
     6241 cgatgtacgc ttggcgtttt tacttgggct tatagctggg tcgcccctaa acgtttttat
     6301 tgcagtattt ttagctagct tagtaggttt ggcagtagca ataccgggga tcgtatctaa
     6361 aaaactcagt gtcacgtcgc acataccgtt tggcccgttt ttaatattgg gcgctagttt
     6421 agccttattt tttggtgata gtctaagcag ctggtacagc accgtacttt taggcctata
     6481 acagtgctac gagctctgtt gcaaaaattg tgcttatggt ataatgcagg gtaatgaaac
     6541 taataaaaaa acaaacaggc tacaccatac tagaaacgct tatcgtgtta acagttacgg
     6601 gtgtgctttt cacgactacc gccctactta tacaggggca ggtagaaaaa acacgttacc
     6661 aagatagcat gcgccaaata cagcagactg ttcaacaagc aataaaagat acagaaaacg
     6721 gttactttcc gggtgccacg gggaatgata cggctggaac ggtgctcgca ggcaaacgca
     6781 tttatttttg tactgatgct ccagacccga acgatgatga aaattctaga caaccatgta
     6841 cagctggaaa aagcatgata agagtagaaa acatatatgc agactccggg ggttcgctag
     6901 acacagacgt agagcttagc aatggcaaag aaacgtacat aacatacccg gggagcatta
     6961 actacaaata tcaccacaaa tctaatggta catacccaag agaatcaaca ggctttgcag
     7021 tgatgtttaa gaatttttca accactgatt atggctcgca aacaacaagc ctttacgagg
     7081 cttatataga tgacaataat ataacaacgc gctcagaaga tgaatggaca aaagaccgta
     7141 ctccacttta ctggaatcag ggtaaacgta tttgttttga cgggtataaa aaaggtagcc
     7201 taattattgg ccgtaacggt agtacaaacg tagaaatgaa tttggatgat ccagaatgta
     7261 ttcactaaaa agacataaat cacaaaaact tatagatcat tctggtgata cgatagttga
     7321 agtacttatt gctattaccg ttcttagttt tgctctcggg ttaagttttg ccactgttag
     7381 cagaagcaat gctggtttac aggcgaataa agaacagtac
//
LOCUS       DAOPLS010000018         7344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_10772_length_7344_cov_5.075731, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000018 DAOPLS010000000
VERSION     DAOPLS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10772_length_7344_cov_5.075731"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PKV52_00830"
     CDS             complement(<1..270)
                     /locus_tag="PKV52_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPD98790.1"
                     /translation="MPQVSPEIETFARIKVVGVGGAGGAAVNRMVEAGVTGVEFIVIN
                     TDAQAIHHSKAQTKLHIGGDSTKGLGAGADPQVGEDAANVSRDDIA"
     gene            complement(396..1628)
                     /gene="ftsA"
                     /locus_tag="PKV52_00835"
     CDS             complement(396..1628)
                     /gene="ftsA"
                     /locus_tag="PKV52_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPD98791.1"
                     /translation="MQKNSYFIGLDVGTSAVRCVIGMPEQNNPDDTMSVIGVGSAQNT
                     GMRKGCVSHVEDVTRAIGEAVSEAERMAGIRVQDVTVNVNGASIGAQMSRGMVSTNSP
                     DRIITRDDIARVEEQATVINMPNNRDIIQIFTKSYRVDGQDNIKDPVGMQGVRLEADI
                     LVVTAGLQCIRTLNDAVDAAQLHIAERTVSSVAAAEVVLDRRQKESGTALVDIGAGTT
                     NIAIIEDGEIVSLAVLPIGGQHLTHDLAIGLKADLEIAEKIKTKYASLKPEAAVSKKV
                     SFTDDGVVYEFDTSRIYDIIIPRFEELLEEVDKVFAKAKRSRRLPGGVVFVGGVANTP
                     GLADFAREQLELHTRIGKLHHIGGLADTVKSTEFATAIGLMNLDGILGLDAMNTQSIT
                     ARNLSSGVFTKIKSFIKR"
     gene            complement(1684..2433)
                     /gene="map"
                     /locus_tag="PKV52_00840"
     CDS             complement(1684..2433)
                     /gene="map"
                     /locus_tag="PKV52_00840"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPD98792.1"
                     /translation="MMTRIKTAQELVKQRESGKMNATILALVGNAIEVGITTKQLADI
                     AAKELRALGGKPTFLGYGGFPDVMCISVNNEVVHGIPGSYIVKDGDIVSIDFGVTYGG
                     MITDAARSFIAGVSTPQKKKLVKGTLAALDAGVGSLSDGVRVGTIAAAIEKEMQRNSF
                     GIVRDLVGHGVGHQLHEDPNIPNFSMRSSGPVLRAGMTIAIEPMSTLGADAVYTAADG
                     WTIVTKDNSLSAHFEDTVLITDTGAEILTRL"
     gene            complement(2430..2978)
                     /locus_tag="PKV52_00845"
     CDS             complement(2430..2978)
                     /locus_tag="PKV52_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPD98793.1"
                     /translation="MIIVVGVAGSGKSTQSKLLATVQGWHWISMGKLLRSSLTGDLEK
                     EMNSGRLLNDNEVEAILETELRKIAGSHKIILDGFPRRVAQTEWLLSTAHAINEKVEA
                     IVHLKAHEEVVLERLLHRGRPDDKPAAINERFLEYEKDIKPLLQVFANKGIPIIEVNG
                     EQPAEVVHADIKKGVVAAGISL"
     gene            complement(2975..3412)
                     /locus_tag="PKV52_00850"
     CDS             complement(2975..3412)
                     /locus_tag="PKV52_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPD98794.1"
                     /translation="MLDRVHNDANAALKAGDKKTTEALRLLYSALKNAQIEDGGVLSD
                     DRAVQVIKKEMKKRIEARDLYAANDRQEQAAQEEFERNLYSQYAPADLSAEQIDAMIQ
                     AVAPMAGEANFAKIMPLVMKEAQGKADGRLVSERVKNYIENIA"
     gene            complement(3412..3627)
                     /gene="rpsU"
                     /locus_tag="PKV52_00855"
     CDS             complement(3412..3627)
                     /gene="rpsU"
                     /locus_tag="PKV52_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPD98795.1"
                     /translation="MIQVTKKDVRESTESLIRRFNRKVQQAGVLAEVKQLQYHEKPIS
                     KRDRRTKAIIRNERKAQKNKRAKLGLR"
     gene            complement(3679..3984)
                     /locus_tag="PKV52_00860"
     CDS             complement(3679..3984)
                     /locus_tag="PKV52_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPD98796.1"
                     /translation="MLDVFITSRVRRKIIVVYAKFPDFKTHVRGLAKLIKEDAGNIQR
                     ELKRLEKVGFLTSEKQGNTKIYSTNKNFPIFKELQGIVLKSQRANQRPTSAGQLRRP"
     gene            4078..5994
                     /gene="priA"
                     /locus_tag="PKV52_00865"
     CDS             4078..5994
                     /gene="priA"
                     /locus_tag="PKV52_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPD98797.1"
                     /translation="MTYIEVSIAAPFTGRKFYTYESVDAIDIGTIVQIPFGTKKSLGI
                     ARNIVKKPTFKTKPIQQITPLIVSAKSLQLLRWMEHFYPYDFGEITNLFIPQNALTKS
                     RSASSVRPKVTATIQPKLTKDQTEALQIIQSNQHVLLHGDTGTGKTRVYLDYANNVLA
                     QGKSVLILTPEIGLTPQLKQTIAAACPYPLHVVHSQNTPAYRKLIWQTAAANTEPAVF
                     VGPRSSLFLPYQNLGLVVIDEAHDNSYKSMQSPKYNAVYVAAQLAKVHKAHFIQSTAT
                     PNVADYAAIQNHQVPIARMTTIAVGPIKASGQVIDLRDKQLFTKHRLIADELLRAIED
                     ALQNGEQTMLFINRRGSARIVQCNACGHIRACPTCGLPLTYHHDTHKMSCHVCNQTYH
                     ATSQCAACGSADLLYFSPGTKGLEQEIQSLFPKARVIRFDLDVSAKDTIQRKLSGLQT
                     GAYDIIIGTQLISKGFDLPHLSVVGVLNADSGFSIPDFRAEEITFQQIYQVTGRVGRG
                     HTLSKFFIQTRQPTHPVIEAALHRNWQDFYDYELHKRKLFTYPPFAYLAVLSITKKTK
                     ATAQKIAQKTYDLLNNTSQLELLGPTPSYHETSHGGYTWQILAKSTSRATLAAALVSL
                     SSEWSIDIDPTSVL"
     gene            6019..6600
                     /gene="def"
                     /locus_tag="PKV52_00870"
     CDS             6019..6600
                     /gene="def"
                     /locus_tag="PKV52_00870"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPD98798.1"
                     /translation="MTQNPRDLIISLPNPHLRQRSKRVGIVTNDTTKLIQTMIDATLD
                     WEDSRKHEVGVALAAPQIDTLLRVVIIREDFEDKTNRTFTILINPEITKYEGDLIEDY
                     EGCLSIQDIYGKVPRYNKVRVKALDANGKEFRFTAEGFLARVVQHEIDHTNGIVFIDH
                     IKDDPAAFYKLDDEGHLEPLNYEADIKANHLLW"
     gene            6563..>7344
                     /locus_tag="PKV52_00875"
     CDS             6563..>7344
                     /locus_tag="PKV52_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HPD98799.1"
                     /translation="MKQTLKPTIFFGSGPVAAKSLALLRVWHPVSLVVTKRTPPHHKE
                     PAPVELYAKTNNLPITYADTKQELDELSLPDANYGIVIDYGVIISEHVIQHFPRGIIN
                     SHFSLLPQWRGADPITYALLSGQSQSGVSLMIIDKGMDTGPLIATKALPIAANDTNQT
                     LTEKLLLLSDKLLQATLPRYVQSEVLPTPQPHTIATYSQKLTKQSGILNSNKLASELA
                     REVRAFAGWPGSRLCYNNVWLTVTQAVASNLAVPVGTLVFHGS"
BASE COUNT         2066 a         1800 c         1381 g         2097 t
ORIGIN      
        1 tgcaatatcg tcacgcgata cattagcggc atcttctcca acttgtgggt cagcacctgc
       61 gccaagccct ttggtagaat ctccaccaat atgaagttta gtctgagcct ttgagtggtg
      121 gattgcttga gcatcagtat taataacaat gaactctaca ccggtcacgc cggcttctac
      181 catcctattt acagcggcgc caccagcacc acctacaccc actaccttta tgcgggcaaa
      241 cgtttcaatt tctgggctta cttgaggcat acgataccac tatcctttcg agatacatta
      301 accttgtgtt gctatctatt gtacttgcca tagaaaatgt attcaagcat ctaaatataa
      361 aaaagatttt gtactaataa ttcgcacact atggactatc tttttataaa gctttttatt
      421 tttgtgaaca caccgctgct taaattacga gcagtaatac tttgtgtgtt catagcatct
      481 aagcctagaa taccatctag attcataagc ccaattgctg tagcaaattc tgtacttttt
      541 accgtatcag ctaaaccacc aatatggtgt aacttgccta ttctggtgtg aagctctagt
      601 tgttcacgag caaaatcggc gagccctgga gtattagcaa caccaccgac aaacactaca
      661 ccacccggca aacgccgtga ccgcttggct tttgcaaata ctttgtcaac ttcttctagt
      721 aattcttcaa atcgtggaat aataatgtca tatatacggg atgtatcaaa ttcatacacc
      781 accccatcgt cggtgaacga tactttttta gatacagcag cctctggctt aagtgatgca
      841 tatttagttt ttattttttc ggctatttct aaatcggctt ttagaccaat agcaagatca
      901 tgcgtaaggt gttgcccgcc tatcggcaat acggcaagcg acactatttc tccgtcttct
      961 ataattgcaa tattggtagt acctgcgcct atgtcaacga gtgcagtgcc agattctttt
     1021 tgtcgcctat cgagtacaac ttcagctgca gccacactag aaactgtgcg ctcagctata
     1081 tgcagttgtg ctgcgtcgac ggcatcgtta agggttcgta tacactgtaa ccctgctgtt
     1141 actactaaaa tatctgcctc taaacgcacg ccctgcatgc caactggatc tttaatattg
     1201 tcttggccat caactctata gctttttgta aaaatttgta tgatatctcg gttatttggc
     1261 atatttatga ccgttgcttg ctcttctaca cgggcaatat catctctggt aattattctg
     1321 tctgggctat tggttgatac cataccacgt gacatctgtg caccaatact ggctccattt
     1381 acattaactg taacatcctg tacacgaata cctgccatac gttcagcttc tgataccgct
     1441 tcgcctatag cacgcgtaac atcttctacg tgcgaaacac agccttttct cataccagtg
     1501 ttttgagcac tgccaacacc tattaccgac attgtgtcat ctgggttatt ttgctcgggc
     1561 atgccaatga cacaccgaac agcagaagta ccgacatcta aaccaataaa atatgagttt
     1621 ttttgcatta tcactagtat accgcctacg cttgggcaat cgcaagcaat ttacagagat
     1681 atattacagt cgtgtaagga tttcggcgcc agtatcggta attaacaccg tatcttcaaa
     1741 gtgagccgac aagctgttat cttttgttac aatcgtccaa ccgtctgcag ctgtgtatac
     1801 agcatcggca ccaagtgtag acattggctc tatcgctatt gtcataccag ctcgtagaac
     1861 agggccgcta ctgcgcatgc taaagttagg aatatttgga tcttcgtgta gttgatgccc
     1921 aaccccgtgc ccaaccaaat ctctcacgat tccaaaacta ttccgctgca tttctttttc
     1981 tatagcggcg gcaatggtac caacccgcac cccatcgctt agcgacccta cgccggcatc
     2041 taaggcagca agggtgcctt taacaagctt ttttttctgt ggagtagaca caccagcgat
     2101 aaaacttctg gcggcatccg ttatcatgcc accatacgtc actccaaaat ctatacttac
     2161 tatgtcgcca tctttcacaa tgtaagaacc gggtatccca tgtactactt cgttattaac
     2221 agatatacac ataacatcag gaaacccacc atagccaaga aacgttggtt taccacctag
     2281 tgcgcgtaac tctttagcag caatatcggc aagttgcttc gttgttatac ctacttcaat
     2341 tgcattgccc actaacgcaa gaattgtggc attcattttg ccactttcac gttgtttaac
     2401 aagttcttgg gcagttttga tacgtgtcat catagcgaaa taccagctgc cacgacacct
     2461 tttttaatat cggcatgaac aacctcggct ggttgctcac catttacttc tataatggga
     2521 atgcccttgt ttgcgaatac ctgaagcagt ggtttaatat ctttttcgta ttctaaaaac
     2581 cgctcattaa ttgctgcggg tttgtcatct gggcgccctc tgtgtagcaa cctctctagc
     2641 acgacttctt catgcgcttt taaatgaact atcgcttcaa ctttttcatt aatagcatgc
     2701 gccgtagata gcagccattc agtctgtgca actcggcgcg gaaacccgtc taatattatt
     2761 ttgtgactac cagcaatctt gcgtagttct gtttctaaaa ttgcttctac ttcgttatca
     2821 ttcagcagcc tgccactatt catttctttt tctaaatcac cagtcaagct acttcttagt
     2881 agcttgccca tagaaatcca atgccagccc tgtacagtgg ccagtaactt actctgcgtg
     2941 ctcttgccag atccagcaac ccctacgacg attatcatgc aatgttctct atgtagtttt
     3001 taacgcgttc agaaacaagt cggccatcgg ctttaccctg cgcttctttc ataactagtg
     3061 gcataatttt cgcaaaattt gcctcaccag ccataggcgc tactgcctgt atcattgcat
     3121 caatttgctc tgcagataaa tctgcggggg catattggct gtataggttc cgctcaaatt
     3181 cttcttgggc tgcttgttct tgcctatcgt tcgctgcata taaatcgcgt gcttcgatac
     3241 gctttttcat ttctttcttt ataacttgca cagccctatc gtcagataaa acgccaccat
     3301 cttcaatttg ggcatttttt aaggcactat acaaaagtct gagtgcttca gtagtttttt
     3361 tatcgcccgc ctttaacgct gcattggcat cgttatgtac acgatctagc attaccgtaa
     3421 gcctagtttg gctcgcttat tcttttgtgc tttgcgttcg ttacgaataa ttgctttggt
     3481 gcgacgatca cgcttgctaa ttggcttttc atgatattgc aattgcttta cttctgccaa
     3541 aacaccagct tgttgcacct tacgattaaa acgcctaata aggctttcgg tggactctct
     3601 cacatctttt ttagtaactt ggatcatact ttaaacaatt ctacggtact ttgacatatt
     3661 tttcaagggt aaaatagatt atggccgccg taattgacct gcagacgtcg gacgctggtt
     3721 agcgcgttgg ctttttagca caataccctg cagctcttta aatattggaa aattcttatt
     3781 cgtagaatat attttcgtgt tcccttgctt ttcgcttgtt aaaaaaccta ctttttctaa
     3841 gcgttttaat tcacgctgaa tattcccagc gtcttcttta ataagttttg caaggccacg
     3901 gacatgggtc ttaaaatcag gaaatttagc atagacaact atgatttttc tgcggacccg
     3961 agaagtaata aaaacatcta acattttttc acctattgtt tatagaacaa cgttaattat
     4021 agacgcattg ccttgttttg taaaaccgtt tttataaata tgccgtatca taactatatg
     4081 acatatatcg aggtgagtat cgcggcacct tttactggta gaaagtttta cacctacgaa
     4141 tcagtcgatg caattgatat tggaacaatt gtacaaatac cctttggcac aaaaaagtcg
     4201 ctcggtatcg caagaaacat agtcaaaaaa ccaacgttta aaaccaagcc tatacaacag
     4261 attacgccgc ttattgtttc tgccaagtcg ctacaactac tgcgctggat ggagcatttt
     4321 tacccatacg actttggtga aattaccaat ttatttatcc ctcaaaatgc attaacaaaa
     4381 tcacgcagtg cgtcaagcgt acgccctaaa gtaacagcaa ccatccaacc taagctcacg
     4441 aaagatcaaa cagaagctct gcagattata caaagtaatc agcacgtttt gctgcatggt
     4501 gataccggca ctggcaaaac acgagtgtat ttagactatg cgaacaacgt acttgcccaa
     4561 ggtaaatcgg tacttatttt aacaccagaa atagggctca cacctcagct aaaacaaacg
     4621 attgccgctg cctgcccgta cccactccat gtggtgcatt cacaaaacac accagcgtac
     4681 agaaagttaa tatggcaaac ggcggctgca aacacagaac ctgctgtatt tgttgggcca
     4741 cgctcaagct tgtttctgcc gtaccaaaat cttgggctcg ttgtcataga cgaagctcat
     4801 gataattcat ataaaagcat gcaaagtcca aaatataatg cagtctatgt ggcagcccag
     4861 cttgcaaaag ttcataaagc acattttata caaagtactg caacgccaaa cgtggcagat
     4921 tacgcagcga tacaaaatca ccaagtacct atcgcccgaa tgacgacaat tgccgttggt
     4981 cctatcaagg cttctggcca ggtaatagat ttacgcgata aacaactttt tacaaaacat
     5041 cgtcttatag cagatgagct actacgtgca attgaagacg ctttacaaaa cggcgaacaa
     5101 accatgcttt ttattaatcg ccgtggcagt gcacgtattg tgcagtgtaa tgcgtgtggc
     5161 catatacgtg cctgccctac gtgcggattg ccactcacct atcatcacga tactcacaaa
     5221 atgtcctgcc atgtttgcaa tcaaacctac cacgccacaa gccagtgcgc agcgtgtggt
     5281 tcggctgatt tattatattt ttcgccaggg acaaaaggtc tcgaacaaga aattcaaagc
     5341 ctatttccta aggcacgtgt tatacggttt gaccttgatg tatccgctaa agacaccata
     5401 caacgcaaac taagcggctt acaaaccggc gcttacgata ttattattgg cacccaactt
     5461 attagtaaag ggttcgattt accccaccta agtgttgtgg gggtgctcaa cgctgatagc
     5521 ggcttttcta ttcctgattt tagggcagaa gaaatcacct ttcagcaaat ttatcaggta
     5581 acaggtaggg ttggtagagg ccatactttg agcaaatttt ttattcaaac aagacaacca
     5641 acccaccctg ttatagaagc tgcactgcat agaaattggc aagattttta cgattacgaa
     5701 ctgcacaaaa gaaagctctt tacctaccca ccattcgcgt atttagcggt gcttagtatt
     5761 accaaaaaaa ccaaggcgac tgcgcaaaaa attgcccaaa aaacctacga tttattaaat
     5821 aacaccagcc agctagagct acttgggcca acacccagct accatgaaac ctcgcacggc
     5881 ggctacacct ggcaaatact cgctaagtca acgtctaggg caacccttgc agcagcactc
     5941 gtaagcttgt ctagtgaatg gagtatcgat attgacccca ctagcgtttt gtaagttacc
     6001 tctgttataa tgtcagctat gacacaaaat ccgcgagacc taataatatc tttgccaaac
     6061 ccccacctta ggcaacgttc aaagcgcgtt ggtatcgtca ccaatgacac taccaagctc
     6121 atccaaacga tgattgacgc aaccctcgat tgggaagaca gtcgcaaaca cgaagtcggt
     6181 gtcgccctcg ctgcgccgca aatagatacc ttgctccgcg ttgttatcat tagggaagat
     6241 ttcgaagaca aaacaaaccg cacatttacc atcctcataa atccggaaat tacaaaatac
     6301 gaaggtgatc ttatagaaga ttacgaagga tgtctaagca ttcaagatat ttacggaaaa
     6361 gttccccgct ataataaagt gcgcgttaaa gcgctagatg ccaatggcaa agaatttcgc
     6421 tttacggcag aaggcttttt ggcacgcgtt gtccaacacg aaatagacca caccaacgga
     6481 attgttttta tagatcatat aaaagacgac cctgccgcat tttataaact agatgacgaa
     6541 gggcatttag aaccgcttaa ttatgaagca gacattaaag ccaaccatct tctttggtag
     6601 tggacccgtc gctgctaagt cgctcgcgct gctacgcgta tggcacccag ttagtttggt
     6661 ggttactaag cgcacaccgc ctcatcataa agagccagca cccgtagaat tatatgcaaa
     6721 aacaaacaat ctacccatta cctatgcaga caccaagcaa gagctagacg aactatcatt
     6781 accagacgca aactatggta ttgtcataga ttatggcgtt attattagcg aacacgtaat
     6841 tcagcacttt ccacgcggaa ttatcaatag ccattttagt ctactgccgc agtggcgcgg
     6901 agccgatcca ataacatacg cgcttttaag tggccaaagc caaagtggtg tgagccttat
     6961 gattatagac aaaggtatgg atactggccc gcttattgct acaaaagccc tgccaattgc
     7021 agcaaacgat acgaaccaaa cgttaacaga aaagttacta ctgctaagcg acaagctctt
     7081 gcaggcaaca cttcctcgct atgttcagtc agaagtactg cctactcccc agccccatac
     7141 cattgccaca tattcacaaa agcttactaa gcagtctggc atactaaact ccaataagtt
     7201 agccagcgaa ttagcccgcg aagtacgtgc ctttgcaggg tggccaggca gtagactgtg
     7261 ctataacaac gtctggctga ccgttacaca agctgtggca agcaacctag cagtacctgt
     7321 cggcaccctt gtgtttcacg gttc
//
LOCUS       DAOPLS010000019         7294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_10869_length_7294_cov_3.516646, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000019 DAOPLS010000000
VERSION     DAOPLS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10869_length_7294_cov_3.516646"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKV52_00880"
     CDS             complement(<1..799)
                     /locus_tag="PKV52_00880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD98800.1"
                     /translation="MLVLVSTAFLVTSIVFEALSFLRVVQQTRSKHVLLILAVATNAI
                     SIVFMFFVTWQFAFLLLFVSLYRLINIAKNIIGRLPELHLRSVTWQSFVALATLQLLV
                     LCGIYVQQLNLMQVSVQDISIGVAMLSLLSAVVILYASVANILRSKPVATRVLADSDL
                     PTLTVAIAARNETPMLVDCLNSVVASDYPKLEVLVFDDNSQDTTADIIKSFAQNGVRF
                     IGWEHTPGDWLSKNRAYQTLLEHASGDYVFFMGVDVRLETSSLRKITE"
     gene            919..1329
                     /gene="secE"
                     /locus_tag="PKV52_00885"
     CDS             919..1329
                     /gene="secE"
                     /locus_tag="PKV52_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPD98801.1"
                     /translation="MATEKQAKAPRKKKMPETVRERAEKGSIKKSKPKKSAKVKATIH
                     RPLSKLRRIGAKEYHPIKTPDNKVGRVLGKRVRFIPKFITESWRELKQVTWPTKREAA
                     SKTGAVIAFAIVFAIFVQVLDLVFSKVVKEIILR"
     gene            1326..1871
                     /gene="nusG"
                     /locus_tag="PKV52_00890"
     CDS             1326..1871
                     /gene="nusG"
                     /locus_tag="PKV52_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPD98802.1"
                     /translation="MSKNRYDSSRQWYAIHTYSGYEEKVAVSIRQRAETLEMADKIFQ
                     VLVPKEKQIEIKNGKRKVVEKRIFQGYVLVEMKLSDDAWYVVRNTPNVTGFVGSGTEP
                     TPVSEDEMDKIKKRMGVDQPKHRIDYSVGEVVNIVDGPFKGFDGSISEIDTQKGKIKV
                     LVNMFGRETPVELDSLQVKKV"
     gene            1877..2035
                     /locus_tag="PKV52_00895"
     CDS             1877..2035
                     /locus_tag="PKV52_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98803.1"
                     /translation="MVVLHENTDPFNDINVWANWANELARSDKDFAKVAVMGTIACEY
                     ETTLDKTD"
     gene            2138..2566
                     /gene="rplK"
                     /locus_tag="PKV52_00900"
     CDS             2138..2566
                     /gene="rplK"
                     /locus_tag="PKV52_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPD98804.1"
                     /translation="MAKAVKTNLKMKIKGGQASAAPPVGSSLGQHGVNMMDFINPFNE
                     QTKDRMGQMLTVHITIYDDRTMEWRIVGTPTDDLIRAKLGIQKGSGRPNTEKVSKKLS
                     DAALTEIAEAKAGDMNTTDIPAVKKIVAGTARSMGVEIEG"
     gene            2568..3029
                     /locus_tag="PKV52_00905"
     CDS             2568..3029
                     /locus_tag="PKV52_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98805.1"
                     /translation="MTTAPGEPGYPAYTGNHFHGSNTSTFDDGVEKENPTDIDNLTKH
                     VPQQRDTTVVNTPEIAQPDIDHKKILEDYRQTAIGFATYELDKSKKTEFLRRAQAIEY
                     YLAIQPEPTYGQFMSLTYEEIVEAYYQAQLHKAYEKSAKNIGRLGVAPLHR"
     gene            3454..4143
                     /gene="rplA"
                     /locus_tag="PKV52_00910"
                     /pseudo
     CDS             3454..4143
                     /gene="rplA"
                     /locus_tag="PKV52_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
     gene            4278..4991
                     /locus_tag="PKV52_00915"
     CDS             4278..4991
                     /locus_tag="PKV52_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidine triphosphate deaminase"
                     /protein_id="HPD98806.1"
                     /translation="MSVFSNTQIRAAIEQGHIICAPYEPKHVSEASLDITLGYHYYKT
                     ELQYTRTIYNPFDREDVERYFAGPYKAMPHAQWATLNGFLPVKNIPDEHPVIALKPGE
                     RILAHSHEFFGIKPPGACELKSRSSWGRNGIAVCFDAGWIDPGYINRLTLEIYNLNKH
                     ETVLIPVGERIGQIIFHDTGPVDGSYGEARNGGFSGKYQQGTDINKIIKTWAPSQMLP
                     KAWQDSRSLPKRIEGVPYE"
     gene            4984..7284
                     /gene="tmk"
                     /locus_tag="PKV52_00920"
     CDS             4984..7284
                     /gene="tmk"
                     /locus_tag="PKV52_00920"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPD98807.1"
                     /translation="MSKGYYIVLEGPEGVGKTTQIELIARQLQKYGYAVRLFREPDSQ
                     SHLSARAIRQITQDPNYPLNTRAEVLLYNAARALSLEVIKQAREQGVICLVDRNYLTT
                     LAIQYYGRGDVPDYDTITSIINFAVGDMEPDLTVILDAPVDELGKRMKRRKHGERFDN
                     LDLGFLDRVRAGYLVEAKQRGYPVVFANESSEKVFRYVWEYVAALFNKQPAKTQPSVH
                     MSSPPPILRAPSSHEPNAQTAAYLDTTANPITITDAGRQKLSSIVTNNESNVYAFNDT
                     LDQVTVAAAMARLSRRGDDMRVTLLDEFVDKAGKDEGLIQRVVTAYGDDSVQQLVGIH
                     MVVENASNLLTKQIEWGRLASYLEQSTRYIYYDQKDASGRYKYYVPTTLSSATKKQYI
                     KTMDQLFDNYSKAVRGITDYLHKTDSTPQKERDGAWRSAIRAQACDAARYMLPVATKS
                     TVGIYASGQAIESLIMRLMADGSKEARLCGKALLTEARKIIPAFLERADKPERGGATV
                     AYRADTRQAMHALVQKLLPETYSSDQSAVTLTHYYPKNELDVVPHMVYEHTTMSEQEA
                     NAAIADLPYKHKVKLFSSYIGERLNRRHKPGRALERITYSWDIVCDYGIFRDLQRHRM
                     VSDLAWQPLTPRYGYDIPELIEKAGMTETYLDSFDTSLELHSNLVAAKKITESQYATL
                     FGHKMRWKATMNAREAMHFIELRTSPQGHPGYRKLAKIMYDKIAEVHPMVSEAMRFVN
                     KDEDAELTRLAAERYTQYKLKVLDKK"
BASE COUNT         2515 a         1436 c         1606 g         1737 t
ORIGIN      
        1 gttctgtaat tttacgtagt gatgacgttt ctagacggac gtctacaccc ataaaaaata
       61 cataatcgcc agacgcgtgt tctaggagcg tttgatacgc tctgttttta gataaccaat
      121 cacctggagt atgctcccac ccaataaaac gtacaccatt ttgtgcaaat gacttaataa
      181 tatctgctgt tgtatcctga gaattatcgt caaatacaag tacttctagt tttgggtagt
      241 cgcttgcaac aacagaattt aagcagtcta ccaacattgg tgtttcgttt ctggctgcaa
      301 tagcaacagt aagtgtgggt aaatctgaat ctgccagcac acgagtggct actggtttac
      361 tacgtaatat gttagctacc gatgcataca atataacgac tgcgcttagt aaagaaagca
      421 ttgccacccc aatacttatg tcttgcacac taacctgcat aagattgagt tgttgtacgt
      481 atatcccaca cagcactagt agctgcaaag tagccaaagc tacaaaagat tgccaggtaa
      541 ccgaacgtaa atgtagctct ggcaagcgcc cgataatatt tttagctata tttataaggc
      601 gatacaaaga tacaaacaac aataaaaatg caaattgcca cgttacaaaa aacataaata
      661 ctattgatat cgcattggta gctaccgcca gtataagcaa tacatgttta gaacgcgtct
      721 gttgcacaac tcttaaaaat gatagagcct caaaaacaat actcgtaaca agaaatgcag
      781 ttgaaacaag cacaagcata tactatagct tagcacgatt tatgctttga cttgagtgat
      841 acaaacgtgc tatattgaca gtgacgacct aaagttattg tactttaggc ttttttaata
      901 ggactggagc gagacttaat ggcaaccgaa aagcaagcta aggcgccacg taagaaaaaa
      961 atgccagaaa cggtacgtga gcgggcagaa aagggatcaa ttaaaaaaag taagcctaaa
     1021 aaatctgcaa aggttaaggc aacgattcat cgccctttga gcaaattgcg tcgtattggt
     1081 gcaaaagaat atcatccgat taaaacaccc gataacaaag tgggtcgggt actagggaag
     1141 cgtgtacgat ttataccgaa gtttattaca gaatcgtgga gagagcttaa acaagttact
     1201 tggccaacaa agcgagaagc agccagtaaa acaggggcag ttatagcatt tgcgattgtg
     1261 tttgccatat ttgtgcaagt gcttgatctg gtatttagca aagtagtaaa ggagataatt
     1321 ttacgatgag taaaaatagg tacgattcta gtcgccagtg gtatgcgatt catacataca
     1381 gtggctacga agaaaaggtt gccgttagta ttcggcagcg tgcagaaacg ctagaaatgg
     1441 ccgataagat attccaagta ttagtgccta aagagaagca aatagaaatt aaaaacggca
     1501 agcgtaaagt tgtagaaaaa cgcatttttc agggctatgt gcttgtagaa atgaagttgt
     1561 ctgatgatgc atggtacgtt gtgcgtaata cgccaaacgt gactggtttt gtcggcagtg
     1621 gtaccgaacc gacaccagtg agcgaagacg aaatggataa gattaaaaaa cgtatgggtg
     1681 tagaccagcc aaagcaccgc atagattact ctgttggcga agtagtaaac attgttgatg
     1741 ggccatttaa aggctttgac ggtagtatta gcgaaataga tactcaaaaa ggtaaaatta
     1801 aggtacttgt aaatatgttt ggtcgcgaaa caccagtaga actagacagc ctccaggtca
     1861 aaaaagtcta aaaattatgg ttgtattaca cgaaaataca gacccattta atgacataaa
     1921 cgtatgggca aactgggcaa acgagcttgc acgttcagac aaagactttg ctaaggttgc
     1981 ggtaatgggt actatagcgt gtgagtatga aactacccta gacaaaacag attaaaactg
     2041 ttacgatata tcggttacgc acttctaata ttaagaagga gagttagtac gcttacgcac
     2101 tacgcagcag aaaactcgtt ataaaggagt ttactatatg gcaaaagccg taaaaaccaa
     2161 tttaaaaatg aaaattaaag gtggccaagc aagtgcagca ccaccagtag gtagtagcct
     2221 gggccaacac ggtgttaata tgatggattt tattaacccg tttaacgaac aaacgaaaga
     2281 ccgcatgggg caaatgctta ccgtgcatat tactatttac gatgaccgca ctatggaatg
     2341 gcgcatcgtg ggcactccaa cagacgacct tattcgtgct aagttgggta ttcaaaaagg
     2401 ttctggccgc cccaatacag aaaaagtcag caaaaagctt agcgatgcag ctttaactga
     2461 aattgccgaa gccaaagcag gcgacatgaa cacgacagac ataccagccg ttaaaaaaat
     2521 tgtcgctggt acagcacgca gcatgggtgt ggagatagaa ggttaatatg acaaccgcac
     2581 caggagaacc aggctaccca gcatatacag gcaatcattt tcatggtagt aatactagca
     2641 ccttcgatga tggagtagag aaagaaaatc ctactgatat agataattta acaaaacatg
     2701 ttccacagca gcgtgacaca acagttgtta acacaccaga aatcgctcaa cccgacatcg
     2761 accacaagaa aatactagaa gactatcggc aaactgcaat aggttttgct acatacgaat
     2821 tagataaaag caaaaaaaca gaatttctac gtagagcgca ggcgattgaa tattacttag
     2881 caatccagcc agaacctact tatgggcaat ttatgtcgtt gacatatgaa gaaatagtag
     2941 aagcctacta ccaagcgcaa ttgcataaag cttacgaaaa aagtgctaag aatattggca
     3001 ggcttggtgt tgcgcctttg catcgatagt tactaggaat attaatcgtg ggaggcaata
     3061 gagccgtttg taccacaaag gagaataatt atggcagaag ccaaaaaacc atctaaaaaa
     3121 gaagcattac ttgcagaagc caaaaagctt aaactaagtg ttactgcaaa aaatactatt
     3181 gtgcaaatag aacaagccat tgcagaggca aaggcagcaa aagaatctac caagaaaatt
     3241 gaagaattgg tagaaaccga aaaaattgca gaagttattg tagaagcaga ggcagaactc
     3301 gcaaaagaag atgttaaaac agctaaggct ggtaagcgaa gcgccaaagg cttaaaagag
     3361 caagaagaaa aagcagcgaa agaagaaaga aaagcccatg cgtctgaaga agaaactaca
     3421 cctgtaaaaa agaaagtagt tacgagaagt cttgttgagc gtaaaggtaa aaagtaccgt
     3481 aaagcaattg aatctatcga taaaacaaaa ctctacagct tgcaagatgc tgccaaggta
     3541 gcgctagcta caagcactac tgcgtttgat gctaccgtag agttgcatgt acgacttggg
     3601 gttgacccac gacatgcaga tcagaatatt agggcgagta ttgttctacc agaaggtaca
     3661 ggcaaaaaag taacagtagc tgtttttgca gaagcagacg acgtagcaaa agccatagca
     3721 gcaggtgcag atattgctgg tgcagaagaa ttcttgcagc aattagataa gggattattt
     3781 aactttgatg tattaattgc tacaccggcc gtaatgccaa aacttggtaa atatgcgcgc
     3841 aatttaggac caaaaggttt aatgccgagc cctaaaagtg gcactgtagc agcagatgtt
     3901 accaaagcag tcaaagaagc caaaggtggt aaagtagaat atcgcgtaga cagcaacggg
     3961 attgtgcatc ttggtatagg taaagttagc tttggtgttg ataaagttgt atcaaacgct
     4021 aaaaccgtat ttgctagcat taaagcagca aagccagcta cggtaaaggg taattacgtg
     4081 atgagtatat atattacgac atctatgggc ccaagcgtta aaatagatac tgcagaaatc
     4141 taaatagccg tcactttata aaaaattagt gcatacacac gctttttaaa aagaagcgtg
     4201 tgtagtatat tagcaactat ttgctatact aatcactaag cggtgggaaa caaaaaataa
     4261 tgtgacgagg taatgaagtg agtgtattta gtaatacgca aatacgagca gccatagaac
     4321 aagggcacat tatttgtgcg ccgtacgaac caaagcatgt cagtgaagca agtctagata
     4381 tcacacttgg ctatcactac tacaaaactg agttgcaata caccagaacc atatataacc
     4441 catttgatag agaagacgtt gaacgctatt ttgcagggcc ttacaaggcc atgcctcatg
     4501 ctcaatgggc taccctaaat ggttttttgc cagtaaaaaa cattccagat gaacatccgg
     4561 tgattgcatt aaaaccaggc gagcgtattt tagcccatag tcatgagttt tttggtatta
     4621 aaccgcctgg ggcgtgcgag cttaaaagcc gctctagttg gggccgtaat ggtattgcgg
     4681 tatgctttga cgctggctgg atagaccctg gttatattaa tcggttaacc ctagaaatat
     4741 acaaccttaa taaacacgaa acagtactta tacctgtggg tgaacgcata gggcaaatta
     4801 tatttcatga tacgggccca gtagatggta gttacggcga agcccgtaac ggtggcttta
     4861 gcggaaaata tcagcagggt accgacatta ataaaattat taaaacctgg gcaccgagtc
     4921 agatgctacc aaaagcatgg caagattcgc ggtcactccc aaaaagaata gaaggagttc
     4981 cgtatgagta aaggctatta tattgttctt gaaggcccag aaggagttgg taaaactaca
     5041 caaatagaac taatcgcccg tcagctacaa aaatatggtt acgcagtccg cttgttcaga
     5101 gaaccagata gtcaaagcca tttatcagcc cgtgctattc gccagattac acaagatcca
     5161 aactatccac ttaataccag agcagaggtg ctactttata acgcagctcg tgcgttgtcg
     5221 ctagaagtta ttaaacaagc ccgcgaacaa ggtgttattt gtttagtaga cagaaactac
     5281 ctcaccacgt tagcgataca atattacggc cggggtgatg tgccagacta tgacacgata
     5341 accagtatta taaattttgc tgtcggagac atggaaccag atcttacagt aattcttgat
     5401 gctcctgttg atgaactagg taagcgcatg aagcgtcgca aacatggtga acggtttgac
     5461 aacctagacc ttggctttct agatagagta agggcaggct atctagtaga agccaaacaa
     5521 agaggctacc cagttgtatt tgcaaacgaa tcgtctgaaa aagtatttag atatgtatgg
     5581 gaatatgtcg ctgcactatt taataaacag ccagccaaaa cacaaccaag tgtacatatg
     5641 agtagcccgc caccaatctt acgtgcacca agtagtcatg aacctaatgc gcaaactgca
     5701 gcgtatttag atacaacagc aaacccaata acaattacag atgcaggacg ccaaaaatta
     5761 tctagcattg tcactaacaa cgaatcaaac gtatatgcgt ttaacgacac gctagaccaa
     5821 gtaactgtag ctgccgcgat ggcacgcctg agccgccgtg gtgatgacat gcgcgtaacc
     5881 ttgctagatg aattcgtaga taaagcaggt aaagacgaag gcttaataca acgagtcgtt
     5941 acagcgtacg gagacgattc tgtccagcag cttgtaggta tacacatggt agtagaaaac
     6001 gcatctaatt tacttaccaa gcaaattgaa tgggggcgtt tagcttctta tttagagcaa
     6061 tcaactcggt atatctatta tgaccaaaaa gatgcgagcg gtcgttataa atactacgtg
     6121 cctacaacgc tcagcagtgc tacaaaaaaa caatatataa aaacgatgga tcaattattt
     6181 gacaattact ccaaggctgt tcggggcatt actgactatt tgcacaaaac agactctacc
     6241 ccccaaaaag agcgtgatgg cgcatggcga tcagctattc gtgcccaagc ctgtgacgcc
     6301 gcccgttata tgctgccagt tgctacgaaa agtaccgttg gaatatacgc atcgggccaa
     6361 gcaatagaaa gtctcataat gagactgatg gctgatggtt caaaagaagc ccgtttatgc
     6421 ggtaaagcat tgcttacaga agcccgcaaa atcataccag cctttttaga acgggcagat
     6481 aagccagagc gtgggggcgc aactgtagcc taccgggcag acactagaca ggcaatgcat
     6541 gcccttgttc aaaaactttt accagaaact tacagctcag atcaatcagc agtaaccctc
     6601 acccattact atcctaagaa tgaactagac gtcgtaccgc acatggtcta cgaacacact
     6661 acaatgagcg agcaagaagc caatgcagca attgctgatc taccatacaa gcataaggtt
     6721 aagctattta gtagttatat tggcgagcga cttaaccgcc ggcacaaacc tggccgtgcg
     6781 ctcgaacgca ttacttatag ttgggatatc gtatgtgatt acggcatatt tagagattta
     6841 cagcgccata ggatggtaag tgatcttgcc tggcaaccgt taactccgcg gtatggctat
     6901 gatatacccg aacttataga aaaggcaggc atgacagaaa cctacctaga ttcatttgac
     6961 acaagcctag aactacatag taacttggta gcagccaaaa aaatcaccga atcacagtat
     7021 gctacgttat ttggccataa aatgcgttgg aaggctacca tgaacgccag agaagccatg
     7081 cattttatag aactgcgtac cagcccacaa gggcatccag gttatagaaa actggcaaaa
     7141 attatgtacg ataaaattgc tgaagtacac ccgatggtgt cagaagcaat gcgctttgta
     7201 aataaagacg aagacgcaga acttacccgg cttgctgcag agcgctatac acaatataag
     7261 ttgaaagtat tagacaaaaa gtagggtttg caga
//
LOCUS       DAOPLS010000020         7202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_11032_length_7202_cov_3.867357, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000020 DAOPLS010000000
VERSION     DAOPLS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11032_length_7202_cov_3.867357"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKV52_00925"
     CDS             complement(<1..568)
                     /locus_tag="PKV52_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98808.1"
                     /translation="MSIRELKRQARQGNKDAVRVYPVRAYGMQLWILLWTVLGFLTTS
                     IILLLKSLIGSPILIILINVPLIVVLHAILPWSKRPKPSLHMAAIVSPYLEWILRKTF
                     PIMRRLEKWVGRWIQPEPFMLIQSKDELLEILRHNADEFDRISKHELKIAENALLFGE
                     KLVQDHMTPLNTVHFVNADDELTPVVIGE"
     gene            756..1406
                     /locus_tag="PKV52_00930"
     CDS             756..1406
                     /locus_tag="PKV52_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPD98809.1"
                     /translation="MDKRFLIIMGIIVVAFLGIFFLGNSNNESQNTQNTGTVSNHTTG
                     NNAKNVELLVYGDFECPACGQFAAIEKQVIDKYKDDIAFTFRHFPIDTIHPNARAAAR
                     AAEAAGAQGKFFEMHDLLYQTQSQWSSQVTTNPQPIFENFAKEIGLDINKYTIDYASD
                     TTNSTINADKQEGNNKGVNGTPTYFINGVKIDNGQITSVEGFSKVIEDAISGANQN"
     gene            1575..3248
                     /locus_tag="PKV52_00935"
     CDS             1575..3248
                     /locus_tag="PKV52_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPD98810.1"
                     /translation="MELFVEISALLVIAAIISLIMRALKQPLIIGYIITGLLVGPAFL
                     GIVKSSDALETFSSFGIALLLFIVGLGLNPKVIKEVGKVSLLTGVGQVVFTTVIGFLI
                     IYGLGYNAVGALYIAIALTFSSTIIILKLLTDKRDQNKLYGKISIGFLLVQDIIATLA
                     LLVASASGNGGIDLEEIIILVAKGVAVVIGVILFVNIVLKRISKFMAKSQEMLFLFAM
                     AWGFGIASLTYLGGFSLEVGALFAGIAIASLPYSQEIALRLKPLRDFFIVLFFISLGS
                     SLEIAGVGPIVWQAILLSLFVLIGNPIIVMIIMGVMGYTRKTSFKAGLTVAQISEFSI
                     IFILLGQKNGQVSDEAVFLVTLVGIITIAISTYMIMYSNQLYDFLAKHLRYFFYDSKQ
                     KAEREHANSYTIVLVGYQRGGREFVKSFREMHKKFIVVDYDPEEIEALERNNIPHIYG
                     DIQDSDLLEEIAIHRSKLVVSTITDFEANIFLIQQLETINPSAVSIVHADNIHQAEQL
                     YAYGASYVMLPHYIGSERMGSFIKKNGFDKIEFRKFREKHLQYLGTHYR"
     gene            complement(3245..4930)
                     /locus_tag="PKV52_00940"
     CDS             complement(3245..4930)
                     /locus_tag="PKV52_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98811.1"
                     /translation="MYQALLTKEKSIHTPKNNDPLAHVRLLQESYDSAVAYHRMRVEF
                     ITDMGERLEDQPFMSQYSYTYDDSEITYDTGLQYMKSLQLSYEAAKHDVARGGFDVYH
                     EERQKAFLQQGELLLDWLQQPSNDTYLLLISPCPPEEELNPNEARRQSFKQKRMMASL
                     QLHAKNSLGQGQTIAFSLDGLTLERLNELLDTLNIHADVASSSLEQVTIPILVTADST
                     PSETASAIVEEYDSLRNHENPSAVYQQGVDTNKSVTEANSFVEKHPEAFDLYRTIIEE
                     VAVSMQVGVTRRLKQTIDDYLVRQYKEKAVPRFLKIDVGQTLTESIGSAMIDYLRKQA
                     IPEYLTQKLHSSNEPQMVNDTHGSYDIGSAGATAMSEGREYDSACASSSNAASVNPAI
                     HSGTQRRELGLMNKAAYETPKPIFSKGAYDVIPIGACPVCGNECGVGIRNKKSGKWYC
                     TRTNCSAFKQDIYAYVFDTDKSLKETKLSSAKQENLAEAKQNHYVLATRKDIRIQIQL
                     NQYAIWTLKNLMYETEVSPSKRAEMIRELIALYQTQGNLVRHVLGMTAHSKTT"
     regulatory      complement(4978..5122)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            complement(5145..5834)
                     /locus_tag="PKV52_00945"
     CDS             complement(5145..5834)
                     /locus_tag="PKV52_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98812.1"
                     /translation="MYTVNKTRSRKKLLIEFFVVLVGLFVILAFVIWFFVFRSNGETS
                     SNFTKTGGTVAVVAPATVDFVVDEFKISLPEGWVLNGKQNPYYNQVYYEFQSKVKDYE
                     NRWLKAYVDVIPQEYALNKLLPITVEGNRIQPGIMSEDCKSFTGAPKPGAGQTSAQTW
                     KATWQDVTFVCDMATQQNHIGTANATNGYNVPIVGPTKGKHTYFFVYIDHNIRPDTTI
                     LKNALKSFEAL"
     gene            complement(5908..6606)
                     /locus_tag="PKV52_00950"
     CDS             complement(5908..6606)
                     /locus_tag="PKV52_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98813.1"
                     /translation="MSKQSSPRFKKSMPFGSIALVVLFVGLAVFGGYFFVKYQNVNTK
                     YKEVTQSVEDRDRQLVQAVSKLYAVPAYEKEKPTVLLVSDKDQLKSASDASAKFFENA
                     ENNDYVLAYKDADISIIYRKGENRIIKIDNYSNFVAAANPINISLLAADTAQKSIQDK
                     ISQQFKNAVVSNKSTPKTAQTKGVVYATSPDFNDAAKQLAQLLGYDVGSLPAGEDAPA
                     QGTDIVIVAPVANQ"
     gene            complement(6710..7048)
                     /locus_tag="PKV52_00955"
     CDS             complement(6710..7048)
                     /locus_tag="PKV52_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98814.1"
                     /translation="MKSPWYEYLNKLDKEKRDIVVTILAIAKKHTLEPEKTMSYGVPT
                     LKSNGNYIIAVAAHKNHVGVYPFGEAPIIAVKHMLGDVKTSKGTICFPYNALPTEEQL
                     AAIVAFNLNR"
BASE COUNT         2083 a         1361 c         1359 g         2399 t
ORIGIN      
        1 actcgccaat aacaacaggt gttaattcat cgtcagcatt cacaaaatgc acagtattga
       61 gtggtgtcat atgatcttgt accagctttt ctccaaataa aagtgcgttt tcggctattt
      121 ttaactcgtg tttacttata cggtcaaatt catctgcgtt atgacgtagt atttctagta
      181 attcatcttt gctttgtata agcataaacg gttctggttg gatccatctt ccaacccatt
      241 tttctaatcg gcgcataata ggaaacgttt ttcggagaat ccattctaaa tatgggctta
      301 ctatagcggc catatgcaag cttggtttgg ggcgctttga ccacggtaaa atagcatgta
      361 acacaacgat aagtggtacg tttatcaaga taattagtat tggactacca attaaactct
      421 ttaaaagtaa aattatactt gtagtcaaaa aaccaagtac cgtccatagc agtatccaga
      481 gttgcatacc gtatgcgcgt actgggtaca cgcggacggc atctttattg ccttgtctgg
      541 cttggcgttt gagttcacgt atactcatga actctaggct tcttataagt aaaatgccat
      601 agaatgtaac accaatcaat acacttagta ataatgtttc tattagttcc actcatgttt
      661 attctacgtt atttttactg taaaatatag cgccaagctt atgtattatt gatacaatag
      721 aataacaaca tgcataacag ataggagtct gaacaatgga taaacgtttt ttgattatca
      781 tgggaataat tgtcgtcgct tttcttggaa tattcttcct tggcaattcg aataatgagt
      841 cacaaaacac tcaaaataca ggtacagtaa gcaaccacac cactgggaac aatgccaaaa
      901 atgttgaatt attagtctat ggtgactttg agtgtcctgc ctgtggccaa tttgcagcga
      961 ttgaaaagca agtgattgat aaatataaag acgacattgc tttcacattc cgccattttc
     1021 caatagatac catacaccct aacgcccgcg cagcagcacg tgcagcagaa gccgcaggag
     1081 ctcaaggtaa attctttgag atgcatgatt tgctatatca aacgcaatcc cagtggagtt
     1141 ctcaggtaac tactaaccct cagcctatat ttgaaaattt tgctaaagaa ataggattag
     1201 acatcaataa atatactatt gattatgctt ctgatacaac aaacagcact attaacgcag
     1261 ataaacaaga aggtaacaac aaaggcgtca atggaacgcc aacctacttt attaatgggg
     1321 ttaagataga caacggccaa attacgagtg ttgaaggttt ttctaaagta attgaggatg
     1381 ctatctccgg cgcaaatcaa aactaattta cgcgttttat aaagatatgt actgacatgt
     1441 caacgccttg ttggtctgta tccaatctag aattactgta cgtataagta ttttgaactc
     1501 ttaatgtatt gagtctataa ataagtagtc aagatcttta tgctttataa tgtagttaag
     1561 gggtagaaaa atatatggaa ttatttgtag aaataagcgc tctattagtt attgcagcca
     1621 tcatttcact gataatgcgt gccctaaaac agcctctcat tattggttac attataactg
     1681 gattattagt agggccagct ttcttgggta tcgtaaaatc ttctgatgca ttagaaactt
     1741 ttagcagttt tggcatagcc ctgctgcttt ttatagtcgg cttaggttta aacccaaaag
     1801 taataaaaga agtaggtaag gtatccctat taactggagt tggccaagtg gtgtttacta
     1861 ccgttatagg ttttttaata atatatggtc ttgggtataa cgcagtaggt gctctttata
     1921 tagcgattgc tttaactttt agcagcacaa ttattatctt aaaacttctc actgacaaac
     1981 gagaccaaaa taaattatat ggaaaaataa gtatcggctt tttgcttgtg caagatataa
     2041 tcgccacact agcgttgttg gttgcgagtg caagcggtaa cggtgggata gatttagaag
     2101 aaataattat tcttgttgcc aagggagtgg cggttgttat tggggtaatt ttgtttgtaa
     2161 atattgtatt aaaacgaatc tctaaattta tggcaaaatc tcaagaaatg ttgtttttgt
     2221 ttgctatggc gtggggattt gggattgcct cgctaacata cctaggcggc ttttcactag
     2281 aagttggcgc tttatttgct ggtatagcta ttgcatcttt accctattca caagaaattg
     2341 ccttacggct aaagccgttg cgcgattttt tcatagtttt attctttatt agtcttggtt
     2401 caagcttaga aatagccggt gttggaccca ttgtctggca ggctattcta ttgtcgttat
     2461 ttgtattaat aggtaaccca atcattgtga tgattatcat gggtgttatg ggatacacca
     2521 gaaaaaccag ctttaaggcc ggtttaacag ttgcccaaat tagtgaattt tctattatct
     2581 ttatattgct cggtcaaaaa aatggccaag ttagtgatga agcagtattc ttggtaacgc
     2641 tcgtaggtat tattactatt gcgatttcta cctatatgat tatgtactct aaccagctgt
     2701 atgattttct tgcgaagcat ttaaggtact tcttttacga cagcaagcaa aaagccgaac
     2761 gtgagcacgc gaacagttat actattgttt tggttggtta tcagcgtggt ggtcgtgaat
     2821 ttgttaaaag ttttagagaa atgcacaaaa aattcattgt agtagattat gatccagaag
     2881 aaatagaagc cttagaacgt aataatattc cacatattta tggtgatata caagacagtg
     2941 atttactaga agaaatcgct attcatcgtt ctaaattggt ggtatctacc atcacagact
     3001 ttgaagcaaa tattttctta atacaacagt tagaaaccat taacccaagc gctgtaagta
     3061 ttgtacatgc cgataatatt catcaggctg aacaattgta tgcgtatggt gctagttacg
     3121 ttatgctccc gcattatatt ggaagcgaaa gaatgggatc gttcataaaa aagaatggtt
     3181 ttgataaaat tgagttcaga aaatttagag aaaaacactt gcaatacctc ggcacacact
     3241 accgttaagt tgtctttgaa tgggctgtca taccaagcac atgccgcacc aaattaccct
     3301 gggtttgata gagggcaatc aattctctaa tcatctctgc ccgcttactg ggcgatacct
     3361 cagtctcata cattaagttc tttaatgtcc atatggcata ttggttaagt tgtatttgta
     3421 tgcgtatatc ttttctcgtt gctaatacat aatggttctg tttcgcttca gcgagattct
     3481 cctgttttgc cgaactaagt tttgtttctt ttaatgattt atctgtgtca aatacatacg
     3541 cataaatatc ttgtttaaat gcactgcaat ttgttcgcgt acaataccat ttaccagatt
     3601 ttttattacg tatcccaacg ccgcactcat taccacaaac agggcaagca cctataggga
     3661 tgacatcata tgcaccttta gaaaaaatag gtttaggtgt ttcgtatgct gccttgttca
     3721 ttagaccaag ttctcgacgc tgggtaccac tgtgtatggc tggattaacg cttgccgcat
     3781 tagaagaact agcacaagca ctatcatatt ccctcccttc actcatggcc gtagcaccgg
     3841 cgctcccaat atcgtaactt ccgtgtgtat cattcaccat ttgcggttcg ttactactat
     3901 gaagtttttg tgttaaatac tctggtatag cctgttttct taaataatca atcattgcag
     3961 aaccgattga ttccgtcagt gtttgtccta cgtctatctt tagaaatcgc ggtactgctt
     4021 tttctttata ctgacgcacc aaataatcat caatcgtttg ttttagtcta cgggttacgc
     4081 ccacttgcat agaaacggca acttcttcta tgattgtccg gtatagatca aaagcttcag
     4141 ggtgtttttc tacaaaacta ttggcttctg taacagactt attggtatct accccttgct
     4201 gatacacagc agatggattt tcatgatttc gtaatgaatc gtattcttct acgattgcac
     4261 ttgcagtttc gcttggagta gaatctgctg taactaaaat tggtattgtc acttgctcta
     4321 agctagacga cgcaacatct gcatgaatat tcagtgtatc tagtagctca ttcaaccttt
     4381 ctaatgttaa gccgtcaagt gaaaatgcta ttgtttggcc ttggcctaaa ctattttttg
     4441 catggagctg tagactcgcc atcatgcgtt tttgcttaaa actttgtcgt cgtgcttcat
     4501 tagggtttaa ctcttcttct ggggggcatg gtgagattag taacaagtac gtgtcattac
     4561 taggctgctg tagccaatca agaagtaatt cgccctgttg caaaaaagcc ttttgacgtt
     4621 cttcatgata cacatcaaat ccaccacgtg cgacatcatg ttttgcagct tcatacgaaa
     4681 gttgtaaaga cttcatgtac tgcaagcctg tgtcatacgt aatctcgcta tcgtcatacg
     4741 tatacgaata ttggctcata aagggctggt cttcaagacg ctcacccata tcggtaataa
     4801 attctacacg cattctatga taggctactg cactgtcgta tgattcttgc aacagtcgaa
     4861 catgtgccag cggatcattg ttttttggag tgtgtataga tttttctttt gtaagtaacg
     4921 cttggtacat gaataatcct gctctatcta caaagcagga ttatatagtc agtccaccaa
     4981 tgacctagta tcctgctcgg gatatatgtt ggtcgtagtc ttagtaatgt atctgactta
     5041 ttgcgtatgt agcaatttac agttgcggca cagtccatga atcacacatg gttccattac
     5101 aaaaagcacg aagtatattg ttaataactt tattatagca aatgctacag cgcttcgaaa
     5161 gattttaaag cattctttag tattgttgta tctggtcgta tattatgatc tatgtaaaca
     5221 aaaaagtagg tgtgtttgcc cttagtaggt ccaactatag gaacgttgta gccgtttgta
     5281 gcgttagccg taccaatatg attctgctgc gtcgccatat cgcatacaaa cgtaacatct
     5341 tgccaagttg ccttccacgt ctgagcactc gtttggccag caccaggctt tggtgccccc
     5401 gtaaagctct tgcaatcttc tgacataata cccggttgta tgcggtttcc ttcgacagtt
     5461 atgggcaata gtttgtttaa agcatactct tgcggaatca catctacata cgctttcagc
     5521 cagcggtttt cgtagtcttt aacttttgat tgaaattcgt aatatacctg attgtaatag
     5581 gggttctgct tgccgtttaa tacccatcct tctgggagtg atattttaaa ctcgtctaca
     5641 acaaaatcaa cggttgctgg tgccacaacc gcgacagtac cacctgtttt tgtaaagtta
     5701 gaagaagttt ctccgttact tctaaatacg aaaaaccata ttacaaaagc caatatgaca
     5761 aacaagccca ctaagactac gaagaattct attaagagct tttttctact tctggttttg
     5821 tttactgtgt acatgtgttc cttttcgtac cagttcactc taataatgac agaattaaac
     5881 gcttatggca atatttgcac atcctgttta ctgatttgcg accggagcta ctattacaat
     5941 atctgtgccc tgtgcagggg catcttcgcc agctggcaaa ctacctacgt cgtatcctaa
     6001 taactgagca agttgctttg cggcatcatt aaaatctggc gatgttgcat acactacacc
     6061 ttttgtttgt gctgtcttgg gggtgctttt attgctaaca actgcattct taaattgctg
     6121 actaatttta tcttggattg atttctgggc agtatctgct gccaataaag atatgtttat
     6181 tgggttagct gctgcaacaa aattactata attatctatt tttataattc tattttcgcc
     6241 tttacgataa atgatagaaa tatcagcatc tttatatgct aaaacgtagt cgttattttc
     6301 tgcattttca aaaaactttg cagacgcatc tgaggcactt tttagctgat ctttgtcact
     6361 cactagtagt actgttggtt tttctttttc gtatgcagga acagcatata atttagaaac
     6421 agcctgcacc aattgtctgt ctctatcttc tactgattgt gttacttctt tgtattttgt
     6481 attcacattc tgatatttaa caaaaaagta gccgccaaac acagctaacc caacaaatag
     6541 aacaacaaga gcaatactgc caaatggcat tgatttttta aaacgtggtg agctttgttt
     6601 tgacatagta tttcagatgt atattataaa gtaagcatag gcacattgca aataatagta
     6661 gtgttctgac actttctgtc tgcaggtgat tcaaagtaaa tatgcgaggc tatctattca
     6721 aattgaaagc tactatagcg gctagctgtt cttcagttgg taaagcgtta tacggaaagc
     6781 aaatagtacc ttttgaggtc tttacatctc caagcatgtg tttgacagca attattggtg
     6841 cttcaccaaa tggatacaca cccacatgat ttttatgagc tgcaacggca ataatatagt
     6901 tgccgttgct ttttaacgta ggaacgccgt agctcattgt tttttctggc tcaagagtat
     6961 gtttttttgc aattgccaga attgttacaa caatatcgcg cttctcttta tctagtttat
     7021 ttaggtactc ataccaagga gatttcatag tactaatgta tcatatagcg aataagtcat
     7081 tcgctataga tagcatcgcc aatataagat accattagtc aatgagacaa aaagctttcc
     7141 aaataattgg tgtgataggt gtaattatat ctgtcgtaat atttataaaa aatccaagtt
     7201 tt
//
LOCUS       DAOPLS010000021         6688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_12168_length_6688_cov_4.519976, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000021 DAOPLS010000000
VERSION     DAOPLS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12168_length_6688_cov_4.519976"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKV52_00960"
     CDS             complement(<1..446)
                     /locus_tag="PKV52_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HPD98815.1"
                     /translation="MKQFSIHMWKHHQELVVLAITSFFLLAGVVQYAIGLTYVLRLTP
                     VVIGIIAALVMLYWHAPPRRKASLIMVAFLVGFIVEIVGVNTGVLFGDYTYGSAMGLK
                     IAGVPILIGITWALVTIAAWQIVSMSPYSRGAKIFLAACLVVIFDL"
     gene            complement(443..1882)
                     /gene="crtI"
                     /locus_tag="PKV52_00965"
     CDS             complement(443..1882)
                     /gene="crtI"
                     /locus_tag="PKV52_00965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPD98816.1"
                     /translation="MKHKQCIVIGAGVAGLSSAALLASKGYDVMVIEQSSTYGGKVGE
                     RMVGHAYFDSGPSVCTDSRVIDLVYTKCGKNPRDYWQYQKLLENTAYFWPSDDTRYTM
                     PIGTARIERSIVHTFHERPKKVRAYSKRTQALYALTARYYIDRRIALKTLFHPKAFVG
                     AMKLLPLLFSTVNTRNSRYFSNPKTVQLFNRYATYSGSNPYKAPAVLQFAAIPELTQG
                     VYFPDGGMRSIATGLYKLCEDVGVKFLFNKKITSLQQSNNFVTSVRVNKQQFLCDTLL
                     YSGDVVRLYELLGDTTHKHKIAQTERSTSAIVFYWEVRGTFKQFGLHNVIFSDDYQKE
                     YAQLQRGEIPQDPTIYLNITSKVDRSHAAKGTENWFVMVNVPAGTKDVAILALKDVVK
                     AKLETMLGGYITILHEHTMSPTDIATSTSAWQGAIYGQASNSLQSLLFRPKNHAKTPY
                     NIYRVGGTVHPGGGIPLAVRSALITTDML"
     gene            complement(1901..3412)
                     /locus_tag="PKV52_00970"
     CDS             complement(1901..3412)
                     /locus_tag="PKV52_00970"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPD98817.1"
                     /translation="MATLKELRDERLRKLDDLHTLGVNPYPASSYRTHIATDVTNRYE
                     ETKGQTVTLAGRITSIRKFGKIAFIVIKDNSGEAQLFLKAGSVQPLDAANSYLGLDNI
                     SLLDSGDFIEAAGTVIKTQTGEPSVEVAKLRLLTKSLRPMPTKQDGFTNKEERLRRRY
                     IDINVNDAVKARFIRRSTFWQATRDYLNEHGFIEINTPVLEHTTGGADATPFVTHMNA
                     LDQDFYLRISHELPLKRLIGAGYEKVYDIGPRFRNENYSDEHLPEHIAMEWYWAYADW
                     RDGMQFMTDMYRYVAKQTFGRLQFTIGSMQVDLAQEWEEWDYATVIQEHYDLNVFDCT
                     LDQVKAALSAHKLEVEKTENIARGIDKLWKNIRKDVVGPVWLVNTPTFISPLSKSNPN
                     NPNTVQRFQPIIAGSELGNGFSELNDPQDQLQRFLEQQKMRDAGDEEAMMLDIDYIEM
                     LEYGMPPACGWGWAERVFWVLEGVSAREGVPFPHLRHEIDDVTKTIYPDINLT"
     gene            complement(3480..3638)
                     /locus_tag="PKV52_00975"
     CDS             complement(3480..3638)
                     /locus_tag="PKV52_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98818.1"
                     /translation="MQRKTSLKLLFIGVGLAVLGAFLALVASLTLVGFITTAIGVALI
                     SLVAFRIV"
     gene            complement(3646..4107)
                     /gene="greA"
                     /locus_tag="PKV52_00980"
     CDS             complement(3646..4107)
                     /gene="greA"
                     /locus_tag="PKV52_00980"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPD98819.1"
                     /translation="MNKAFKLTQSGIDELQAELDDLIAQRPEITERIKTAREMGDLSE
                     NAEYASARSEQDRCELRIKEIEHIIKNAEIIKQPKSANKVHLGSKVVLKSDGGKTQQY
                     QIVGTVEANPLEGKISDESPIGLALMNKTVGDSVEIVTPAQTTLYKVTDIS"
     gene            complement(4192..5895)
                     /locus_tag="PKV52_00985"
     CDS             complement(4192..5895)
                     /locus_tag="PKV52_00985"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPD98820.1"
                     /translation="MRVTELFTKTSKTVPADETAKNAQLLIQAGFIHKEMAGAYAYLP
                     LGLRVIENIKTIVREEMNATGSQELLMTVLQPKEIWEKTNRWDDAVVDNWFKTTLLNG
                     TELGVGLTHEEPIVDAAKGSISSYKDLPKSVYQIGTKFRNEKRAKSGLLRGREFIMKD
                     AYTFAKDQTQHEEIYEKIAAAYHKVYERLGLGDSTYRVKADGGIFTKRYSDEFQTASP
                     LGEDTIFTVPDTDIFFNAEIAPSQATQGVQPTDMLPMEHLKTPGVTGVQHLSEVLGVP
                     VERTVKTMLYIVDGVLTAVAVRGDYDVNEIKLRTALGATKVALADEATIKETTGAAVG
                     YAGLIGLPNDVQIIVDDSLKTLVNFELGANKTDYHCINVNWGRDLPLPEKLYDVKMAK
                     SGDIHPETGKVYTTFKGVEVGNIFPLETKFTDALDLYYSDVNGERKQIIMGSYGIGVS
                     RLMGVIAEHFADDKGLVWPENIAPFLVYLVQIGTDEAVAQAAKNLYTELAAKGIEVLW
                     DDRDARPGEKFADADLYGIPYRIVVSNKTLQSNQYEVKKRSEKDSEMISKEKLVNMFT
                     T"
     gene            complement(5991..>6688)
                     /locus_tag="PKV52_00990"
     CDS             complement(5991..>6688)
                     /locus_tag="PKV52_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98821.1"
                     /translation="GRRHDENSQILGYFNRTDEVVINISGIKVKALNAAVSYNETLSA
                     VLVHEVLGHALEKLIFNNNESFNEHFDYSEERITGEIYTSIHQDISPIEETLTETTQP
                     VREYGWMNPAEDLATTVDGLVTRVMGWDLEKIKNFKSNPDRYREEIIMRMMDMAAAKA
                     REMGASGVVGSRITFILDEHGNTKVRPAQQPHVTTVTGEEYIKKTIDDAYKEIIETIP
                     RTIRVYLAPEMVM"
BASE COUNT         1689 a         1530 c         1319 g         2150 t
ORIGIN      
        1 attaaatcaa atattactac taaacaggct gctaaaaata tctttgctcc cctgctatac
       61 gggctcatac ttactatttg ccacgcagca atggtcacta aggcccaagt aatacctatg
      121 agtatgggga ctcccgcgat ttttaacccc atcgcactcc cgtacgtata atcaccaaac
      181 aaaactccag tgtttacccc cactatttct acgataaagc ctactaaaaa agctaccata
      241 ataaggctag cttttcgtcg tggtggtgca tgccaataca gcatgacgag tgctgcaata
      301 atacctatca caacaggcgt caggcgcaag acgtatgtga ggcctatcgc gtactgcaca
      361 acaccggcca gcaaaaaaaa gctggtaatt gccagtacga caagctcttg atgatgtttc
      421 cacatatgta tactaaattg cttcatagca tatccgttgt tatgagggcg gatcgtaccg
      481 ccaaaggtat tccaccacct ggatgcacag taccacctac cctatatata ttgtacggtg
      541 ttttggcatg atttttaggc ctaaatagca aggattgtaa tgaattactc gcttggccgt
      601 aaatcgcccc ttgccatgcg ctcgtgctcg tagcaatatc tgtagggctc atggtatgtt
      661 catgtaaaat cgtgatgtaa ccgcccaaca tcgtttctag tttggcttta acgacatcct
      721 taagagcgag tatggcaacg tcttttgtac ctgcgggtac attaaccatg acaaaccagt
      781 tttcggtacc ttttgcagca tgtgagcggt cgactttgct ggtgatattc aagtatatcg
      841 ttggatcttg tggtatttcg ccacgttgca attgggcata ttctttttgg taatcatcgc
      901 taaaaataac gttgtgtaaa ccaaattgct taaaagtacc gcgtacttcc cagtaaaaaa
      961 caatcgcaga ggtagaacgc tcagtttggg caatcttgtg tttatgcgtt gtatcaccaa
     1021 gtagttcata caggcgcaca acatcaccac tatatagtaa ggtgtcacat aaaaattgtt
     1081 gcttattaac acgaacagac gttacgaaat tattgctctg ctgcaaagac gtaatttttt
     1141 tattaaacaa aaacttcacc cccacatctt cgcagagttt gtatagccct gtcgcaatag
     1201 accgcatacc tccatcagga aaataaacac cctgggttag ttctggtatg gcggcaaatt
     1261 gcaatacagc tggggcttta tatgggttac taccactgta cgtagcgtag cggttaaaga
     1321 gttgtactgt cttggggtta gaaaaataac ggctattgcg agtattaacc gtactgaaaa
     1381 gcagtggcag taatttcata gcaccaacaa atgcctttgg atgaaataat gttttcagcg
     1441 caatccttct gtctatataa tagcgtgccg ttagtgcata taacgcttgg gtacgctttg
     1501 aataggcccg tacttttttg ggccgctcat gaaaagtgtg cacaattgac ctttctatac
     1561 gtgccgtgcc tatgggcatc gtatagcgcg tgtcgtctga aggccaaaaa taagctgtgt
     1621 tttctaacaa tttttggtac tgccaataat ctctagggtt tttaccacac tttgtgtata
     1681 ccaaatctat gacacgtgag tcggtgcaaa cacttggacc actgtcaaaa taggcgtgcc
     1741 ctaccatacg ttcgcccact ttaccaccgt aggtgctact ttgctcgata accataacat
     1801 cgtagccttt actggcaagc aaagccgcac tgctaagccc tgccacacca gcgcctatga
     1861 cgatgcattg cttgtgcttc acgtacggtc ggctcctttt ttatgtaaga ttaatgtcag
     1921 gatagatggt ttttgtaacg tcgtctattt cgtggcgcaa atgtggaaac ggcacacctt
     1981 cacgcgcact tacaccttct agaacccaaa aaacacgctc tgcccatccc cagccacatg
     2041 ctggtggcat gccatattcg agcatttcaa tgtaatcgat atcaagcatc atcgcttctt
     2101 cgtcacctgc atcacgcatt ttttgctgtt ctaaaaaccg ctgcaactgg tcttgtgggt
     2161 cattgagctc actaaaccca ttaccaagtt cactaccagc gataataggc tgaaagcgct
     2221 gtacggtgtt agggttattt ggattgctct tactaagcgg actaataaac gttggcgtat
     2281 ttacgagcca caccggccca actacgtctt tgcgaatatt cttccatagc ttatctatgc
     2341 ctcgggcgat attttcggtc ttttctactt ctagtttgtg agctgaaagc gctgccttca
     2401 cttggtctag ggtgcaatca aacacgttta agtcatagtg ttcttgaata accgtagcat
     2461 aatcccactc ttcccactct tgtgccaagt ctacttgcat agaacctata gtaaactgca
     2521 gtcggccaaa tgtttgctta gcgacatacc gatacatgtc tgtcataaat tgcatgccat
     2581 cacgccaatc ggcgtacgcc caataccatt ccatcgcgat atgttctggc aaatgctcat
     2641 cgctgtaatt ttcgttccga aaccgtgggc caatatcata taccttttcg tacccagcac
     2701 caatgagacg ctttagtggt aattcatggc tgatacgcaa gtaaaaatct tggtcaagcg
     2761 cattcatgtg tgtcacaaaa ggcgtagcat cagcgccacc tgtcgtatgt tctagtactg
     2821 gcgtattaat ttctataaag ccgtgttcat tcaaataatc tctggttgcc tgccaaaatg
     2881 tactacgacg aataaaccgc gccttcacag cgtcattcac attaatgtct atgtaacgtc
     2941 ggcgcaggcg ttcttcttta ttggtaaagc cgtcttgctt ggttggcatg ggccgtaaac
     3001 tcttggtaag caagcgtaat tttgcaacct ctactgaagg ctcgcctgtt tgtgtcttaa
     3061 taacagtacc agcagcctct ataaaatcac cactatctag cagactaata ttatcgagcc
     3121 ccaaataact attggctgcg tcaagcggtt gcacgctacc agcttttaag aaaagctggg
     3181 cctcgccaga gttgtctttg atgacgataa aagcaatctt tccaaacttt ctaatacttg
     3241 taattcgccc tgcaagggtt accgtttgac cttttgtttc ttcgtatcta ttggtcacat
     3301 cggtagcaat gtgggtacga tacgaagacg ctgggtatgg attaacgcca agtgtatgta
     3361 aatcgtctag tttgcgcagg cgttcgtcgc gcagttcttt tagtgtagcc attgtaaatt
     3421 agtatacagc aaaatgagca ttttgccttt taggtgcctc tattgaaaaa tagcttaacc
     3481 tacactatac gaaatgcgac taatgatatg agtgctacgc ctatagcagt agttataaag
     3541 cccacaagcg tgagcgacgc aacaagcgct aagaatgcac ctaaaactgc cagccctacc
     3601 cctatgaata gcagttttaa ggaggttttt ctctgcatta tgtgtttagg aaatgtcagt
     3661 tactttataa agggttgtct gggcaggagt tacaatttct acgctatcac cgacagtctt
     3721 gttcattaag gcaagcccaa ttggtgattc atcactaatt ttaccttcta gcgggttagc
     3781 ttctaccgta cccacaattt gatactgctg cgttttgcca ccgtcgcttt ttaggacaac
     3841 tttgctgcca aggtgtactt tgttagcaga ttttggctgc ttaataattt cggcattttt
     3901 aataatgtgt tcaatttctt taatacgtag ctcgcaacga tcttgttcac tgcgggcact
     3961 ggcgtattct gcattttcac ttaaatcacc catttctctc gctgttttta tgcgttcagt
     4021 aatttctggg cgttgggcaa ttaaatcatc tagttctgcc tgtagttcgt ctattcctga
     4081 ctgcgttaat ttgaatgctt tattcattct accctcttat ttttccgtta aatgagctgc
     4141 gttgctcctt tttcacacct gtctgcagcc gtagtagtat agcatatggt actaggttgt
     4201 aaacatattg acaagttttt cttttgatat catttctgaa tctttttcag aacgtttttt
     4261 tacttcgtat tggttgctct gtagtgtctt attacttacc acaatgcggt acggtatgcc
     4321 atataaatcg gcatcggcaa atttttctcc tgggcgagca tccctatcgt cccacaggac
     4381 ttctataccc ttggcagcca gttctgtgta taagttttta gctgcttgcg ctacagcttc
     4441 gtctgtacct atttgtacaa gatacactaa aaacggtgcg atgttttctg gccaaaccaa
     4501 ccctttgtca tcggcaaaat gctctgctat aacccccatt aatcggctaa cccctatacc
     4561 atagcttccc atgataatct gttttcgttc accgtttacg tctgaatagt ataaatctag
     4621 tgcatctgta aattttgttt ctaaggggaa tatattgccc acttctacac ctttaaaggt
     4681 cgtgtatact ttgccagttt ctggatgaat atcaccagat ttggccattt taacgtcgta
     4741 cagcttttct ggcagtggca aatctctgcc ccagtttacg ttaatgcagt gatagtctgt
     4801 tttatttgca ccaagctcaa agttaacgag tgtttttaac gaatcatcca cgataatctg
     4861 aacatcgttt ggcaaaccaa ttaatccggc gtagcctacg gctgcaccag tggtttcttt
     4921 aatcgttgcc tcatctgcta aagccacctt cgtagcacca agcgctgtcc ttagcttgat
     4981 ttcgtttaca tcgtaatctc cccgtactgc cacggcagta agtaccccgt ctacaatata
     5041 gagcattgtt ttgactgttc tctctacggg tacgcctaag acttctgata gatgctgcac
     5101 gcccgttaca ccaggagttt ttagatgctc cattggtaac atatcggtcg gttgtacacc
     5161 ttgcgttgcc tgagacggtg ctatttctgc attaaaaaat atatcagtgt ctggcactgt
     5221 aaatattgta tcttcgccca aagggcttgc cgtctgaaat tcgtcgctat atctttttgt
     5281 aaaaatccca ccatctgcct ttacgcggta tgtgctatcg cctagaccta agcgttcata
     5341 tactttgtgg taagcagccg caattttttc atagatttct tcatgctggg tttgatcttt
     5401 ggcaaatgta taggcgtctt tcataataaa ttctcggcct cttaataggc ccgatttcgc
     5461 tcgtttttca ttacggaact ttgtacctat ttggtagaca cttttaggca aatctttata
     5521 agaagaaatt gaacccttgg ccgcatctac gattggttct tcgtgggtta agcctacccc
     5581 tagctctgtg ccgttaagta acgttgtttt aaaccagtta tctaccactg catcgtccca
     5641 gcgattggtt ttttcccaaa tttctttggg ttgcagaacc gtcattaata attcttggct
     5701 ccctgtggcg ttcatttctt cacgcacaat ggttttgatg ttttctatca cgcgcaagcc
     5761 aagtggcaaa taggcgtagg ctcctgccat ttctttatga ataaaaccgg cttgtataag
     5821 aagttgtgcg tttttagcgg tttcgtctgc tggtactgtt ttgctggttt tggtgaatag
     5881 ttctgttact ctcatgtcta gcaattatac cagaagtact gtaccaatag gctgttgtta
     5941 tgggtgtgta taatataaaa atgtagagat ctaaataaac tcttttgttt ttacataacc
     6001 atttcaggcg ctaagtatac gcgtatagta cgtggaatag tttcgattat ttctttatat
     6061 gcatcgtcta ttgtcttttt gatatattct tcgcccgtta cggttgtaac gtgtggttgt
     6121 tgcgcaggtc ttacttttgt attgccatgc tcatctagta tgaatgtaat gcggctcccc
     6181 acaaccccag aggctcccat ttctcttgcc ttagcggcag ccatatccat catacgcatg
     6241 attatctctt ctctatatct atcaggatta cttttaaaat ttttaatttt ttctaagtcc
     6301 cagcccatca ctcttgttac taatccatct acggtagtag ctaagtcttc agcgggattc
     6361 atccaaccat attcacgtac cggttgtgta gtttcagtaa gcgtttcttc tatagggctt
     6421 atgtcttggt gtattgaggt atatatctcg cccgtaatac gttcttcaga gtagtcaaag
     6481 tgctcattaa atgattcatt attattgaat atcagttttt ctaaggcgtg tcctagtact
     6541 tcatgcacga gtacagctga aagtgtttca ttatacgaca ctgcggcatt tagggctttt
     6601 actttaatgc cgctaatatt gataacaact tcatctgttc tgttaaaata gcccagaatt
     6661 tgagaattct cgtcgtgtct tcttccta
//
LOCUS       DAOPLS010000022         6407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_12933_length_6407_cov_4.172072, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000022 DAOPLS010000000
VERSION     DAOPLS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12933_length_6407_cov_4.172072"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKV52_00995"
     CDS             complement(<1..208)
                     /locus_tag="PKV52_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98822.1"
                     /translation="MTYLYYGENTQERDAAVKALLGSFIAVNGDMAVDVIDTEMAEPA
                     DVIDAVTTVPFLSPKRLVVLRYVSS"
     gene            complement(210..1091)
                     /gene="mutM"
                     /locus_tag="PKV52_01000"
     CDS             complement(210..1091)
                     /gene="mutM"
                     /locus_tag="PKV52_01000"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPD98823.1"
                     /translation="MPELPEVETVRIGLKKLIIGKTIVNVSFDWPKSFPNDPQQTAAF
                     ILNARIQNVRRRAKVLMIDVSTDYSLVIHLKMTGQLVYRGEQTFGAGHPNDSLVGDLP
                     DKSTRVIITFADNSQLFFNDQRKFGWMRLIPTLEIPNIDFMKKVGPEPLEPSFTLQVF
                     QDQLLRRKNTNIKAALLDQTVVAGIGNIYADESLFMAGIHPKSIVGAIPKRRINLLHD
                     SVVHVLQLSINKGGSTDKNYVNAEGKKGSYMSFAKVFRQEGNICPNGCGSIIEKIKVA
                     GRGTHICKKCQKLYKVA"
     gene            1178..1414
                     /locus_tag="PKV52_01005"
     CDS             1178..1414
                     /locus_tag="PKV52_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98824.1"
                     /translation="MDSIKDLLAKKDVDEPTEVTALKHYCKQQFNFTAKISLRNDILW
                     LSVPNGILATELRMRQTDIITRCGITKKFRIKIG"
     gene            complement(1411..2568)
                     /locus_tag="PKV52_01010"
     CDS             complement(1411..2568)
                     /locus_tag="PKV52_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD98825.1"
                     /translation="MNREDPWLLEQPSSHSSYPIRHEEITIKTSSFRKQDISVLLYHP
                     ETPANNPLERELVLCESGWTETRLDKQARSIGRVGLHAACFNHPRRGALRDLWSLLVA
                     SRNLRANWQELSNAIWEEKDLERAEGVLIDMLADQVADEDDRKTLFSAAVSAGKSRRD
                     NLLAVTDRLREENFDLHLLGHSLGGIDVTMATKAAPQHVKSLSLAGPGGVMINDSVGG
                     IIPRVAATVVGERAEVFHNVRELARMAVNTVLFIAENPALTIYEGGYAATSRLEKSLW
                     DIMADYGIPTTLILGEKDKMFPTDIVTQDIQPIPFAGTVIIPGAGHNMTLHQADIVAR
                     IMRNIAMDVDDRRSGRLEVNMKNALAMRAHLRPTDTMSMTDVEIDLFKRND"
     gene            complement(2606..3184)
                     /locus_tag="PKV52_01015"
     CDS             complement(2606..3184)
                     /locus_tag="PKV52_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPD98826.1"
                     /translation="MIYRAIIFDFFGVVGQSTYQLVGEDYTYTEAQNAQLHDLHKAFD
                     NGFVGDQEFLQTYARIIGLPYEKFIKKYYTSEQRFRNSYAVLALVESLKKDYKVGLLS
                     NVGQDAYTKFIEPIVHHFDEVVTSYDAQLAKPERAIFELMAHKLGVDVSECIMIDDSE
                     SNCEGARAAGMQAICFTTLDNLKQTLRIYLIQ"
     gene            complement(3242..3676)
                     /gene="dut"
                     /locus_tag="PKV52_01020"
     CDS             complement(3242..3676)
                     /gene="dut"
                     /locus_tag="PKV52_01020"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPD98827.1"
                     /translation="MNVLFKKLRADAVLPQYQTDDSAGMDVCAAIDEPVVLGHLDRAT
                     IPCGFAMAIPRGYEIQVRARSGLAAKHGIGLVNGIGTIDSDYRGELGVILINYSKDSF
                     TIKNGDRIAQIVLAQTERIHWKTVDNLDTTGRGQGGYGSTGV"
     gene            complement(3820..4407)
                     /locus_tag="PKV52_01025"
     CDS             complement(3820..4407)
                     /locus_tag="PKV52_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98828.1"
                     /translation="MDPRVIRIIRIVLILLVLFLFIWLIIWLFRPSKPSNEPAQPTTP
                     EATAQQEQLQNVRYVQDGEITGPEQHYSIVITVNATSRRIDIYNGYNVGPMRSESYIN
                     NQASYDAYYSALKNVGFFSTRDNTDNVDRTSYCPLGIRYSYIAGNDLTDPVLNSWSAS
                     CNRRAGTFAGNAGNVKTLFKNQIPDYATLTKGINL"
     gene            complement(4469..4969)
                     /locus_tag="PKV52_01030"
     CDS             complement(4469..4969)
                     /locus_tag="PKV52_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98829.1"
                     /translation="MNTFIGGITPQLDFPRQDLSDDNAQMLEVMLSNPHVLNVFHETA
                     ESVNAVYRVGHPIVKITIEQLYDSQHAWAASVGTAVYEAIAALVQKPTTDISPVMLEH
                     LQSPDTKEALVYTLQSELQAFYRDMPNTAAVVESASSRVTADTTYAVLGAVVTRNFEL
                     VDANYQ"
     gene            5040..5408
                     /locus_tag="PKV52_01035"
     CDS             5040..5408
                     /locus_tag="PKV52_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HPD98830.1"
                     /translation="MTSQKQRWPKYIYRVGEEPDARVSLANERTALAGLRTALALVAT
                     GLGAAALKNSVSVPVAFSVAAIILSIGGAVLAIVSVFRWANVEIAIRTKTPLPAPKGL
                     FTLAVVILIVGIISILAIIT"
     gene            5424..5705
                     /locus_tag="PKV52_01040"
     CDS             5424..5705
                     /locus_tag="PKV52_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98831.1"
                     /translation="MKKEPSNASGRTLFAWRRTALALVAIAALAMKLAGITQQPIGII
                     TATIALFSALVVAHLSSPKQTPLHSSYARLWLSATSVVLVAISALVCFW"
     gene            complement(5680..6183)
                     /locus_tag="PKV52_01045"
     CDS             complement(5680..6183)
                     /locus_tag="PKV52_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD98832.1"
                     /translation="MTHLSSHTTQYTARNNYGIGAGGVVYKRINTGVQFLLLGRTSAN
                     AEVTYHLPKGTLHIDEPIEACALREITEEAGVKVALKTYLGGVCASYDLDGHHYEKII
                     HYYAAEYVSEADPMDAEHDFKEWCDTTDALEKLSHNPKREDKLIEKCSTYLRSHKKPF
                     TKNKPKH"
     gene            complement(6200..>6407)
                     /locus_tag="PKV52_01050"
     CDS             complement(6200..>6407)
                     /locus_tag="PKV52_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPD98833.1"
                     /translation="LAMIKLENALPKTAKQLLQIHDSILVEVLDNDIGIAADIMKSTM
                     ETIHPQLGINLKVDVKTGKHWGEL"
BASE COUNT         1741 a         1432 c         1255 g         1979 t
ORIGIN      
        1 tactacttac gtatcgtaaa accactaagc gcttaggcga caaaaacggc acagtagtaa
       61 cagcatctat aacatcagca ggctctgcca tttctgtgtc tataacgtct accgccatgt
      121 cgccattaac agcaataaaa gaaccgagca acgcctttac ggctgcatct ctttcttggg
      181 tgttttctcc gtaatataaa tatgtcatgc tacgctactt tatacagttt ttggcacttt
      241 ttgcatatgt gcgtgcccct gcctgctact tttatctttt ctattatact gccacaaccg
      301 tttgggcaga tgtttccttc ttgtctaaat acttttgcaa aactcatgta actacccttt
      361 ttgccttctg cattcacata gtttttatca gttgatccac ctttattaat acttagttgc
      421 aaaacatgca ctaccgaatc gtgcaataga ttgatacgcc gcttagggat tgctccgacg
      481 atgctttttg ggtgaatacc agccataaac aacgattcat cggcatagat attgccaatg
      541 ccagctacga cagtctggtc tagaagcgct gctttgatgt tcgtgttttt acggcgcagt
      601 aattggtcct gaaatacctg cagagtaaag ctaggttcta gtggttctgg gccaactttt
      661 ttcataaaat caatatttgg tatttctagc gtagggataa gcctcatcca gccaaattta
      721 cgttgatcat taaaaaatag ctgggaatta tctgcaaaag ttatgataac tcgcgtagat
      781 ttatctggta aatctcctac caagctatca ttcgggtgac ctgcaccaaa tgtttgttca
      841 ccgcggtata ccaactgccc cgtcattttt aaatgaataa ctaagctata gtctgtggat
      901 acatctatca tgagcacctt ggctctgcgc cggacgtttt gaatgcgtgc gtttagtata
      961 aaggccgctg tttgctgcgg atcattagga aaactttttg gccaatcgaa cgaaacatta
     1021 acaatggttt taccaatgat taactttttc aaacctattc tcactgtttc tacttctggt
     1081 aactctggca tgcttttagt ctacagcaga ctactggcag attacagctt aagaactact
     1141 gtttacgtac gtctcgcaac tcgttactat taagtagatg gacagtatta aagatttact
     1201 tgctaaaaaa gacgtagacg aaccaacaga agtaactgct ctaaagcact attgcaagca
     1261 gcagtttaat ttcactgcta agatttctct tagaaatgat attttatggc tcagtgtacc
     1321 taacggcata ctagccaccg aactacgtat gcgccaaaca gatatcatta cccgttgtgg
     1381 tattaccaaa aaatttcgta ttaaaattgg ttagtcgtta cgtttaaata aatctatctc
     1441 tacatctgtc atactcatgg tatcggtagg tcgtaaatga gctcgcattg caagcgcatt
     1501 tttcatattt acttctaacc tacctgagcg cctatcatca acatccatag cgatatttct
     1561 cataattcta gccacaatat cagcttggtg cagggtcata ttatggcccg cgccaggaat
     1621 tatgactgtc cctgcaaaag gtataggctg tatatcttgt gtaacaatgt ctgttgggaa
     1681 cattttatct ttttcgccca atatcaaagt tgtgggtatg ccgtagtcgg ccataatatc
     1741 ccataatgat ttttctaaac ggctcgttgc cgcgtagcca ccttcgtaaa tggtaagggc
     1801 tgggttttct gctataaaga gtaccgtatt aactgccatt cgggcgagtt cacgtacgtt
     1861 atgaaacacc tctgctcttt cgccaacaac tgtggccgca actcgcggaa ttatcccacc
     1921 aacagaatcg tttatcatca caccaccagg acctgcaagc gagagcgact taacgtgctg
     1981 tggtgctgct tttgttgcca tcgtaacgtc aattccacct aaggaatgtc caagtaaatg
     2041 caagtcaaag ttttcttcac gcagtctatc cgttactgcc agtaaattgt cgcggcgaga
     2101 tttacctgca gacacagcag cactaaacag cgtttttcta tcatcttcat cggcaacttg
     2161 atctgcgagc atatcaatca atacaccttc ggcacgctct aggtcttttt cttcccaaat
     2221 ggcattagaa agttcttgcc agttcgcccg aagattgcgg ctagctacaa gcagtgacca
     2281 taaatcacgc agggctccac ggcggggatg attaaaacat gctgcatgta aaccaacccg
     2341 gccaatagac ctggcttgtt tgtctaaccg ggtttcggtc cagccagatt cgcacaaaac
     2401 aagctctctt tcaagcggat tatttgcagg ggtttctggg tgatacaaaa gaaccgaaat
     2461 gtcttgcttg cggaatgatg aggtttttat ggtaatttct tcgtggcgaa tggggtatga
     2521 actatgtgag ctaggttgct ccagaagcca cgggtcttct ctgttcataa ttatctagtg
     2581 gtatattata ccatgtttag caaacttatt gtattaaata tatacgaaga gtttgtttta
     2641 aattgtctaa tgttgtaaaa caaatagcct gcataccagc agcgcgtgca ccttcgcaat
     2701 tagattctga atcgtctatc ataatacatt cgctgacatc tacgccaagc ttgtgcgcca
     2761 taagctcaaa tattgcccgt tctggtttgg ctaattgtgc atcatagctg gtaactacct
     2821 cgtcaaaatg atgaacaatt ggttcaataa attttgtata cgcgtcttgc cctacgttac
     2881 taaggagccc aactttataa tcttttttta atgattccac aagagccaaa acagcataag
     2941 aattacgaaa tctctgttca gatgtgtagt atttttttat aaatttttcg tatggcaacc
     3001 caataatacg tgcgtacgtt tgcaaaaatt cttggtcacc gacaaagccg ttatcaaatg
     3061 ctttgtgtaa atcatgcagt tgtgcgtttt gcgcttctgt atatgtatag tcttcaccaa
     3121 cgagctggta ggtagactgc cctacaacgc caaagaaatc aaaaattatt gctcggtata
     3181 tcattgcact attgtagcac taccctgtgg cgttacgtgc gctgcatata aggcacaaca
     3241 gctacacacc tgtgctgcca tagccccctt ggccacgacc ggtagtatca agattatcta
     3301 cagttttcca gtgtatacgt tcggtttgcg ccagcacaat ttgggcaatt ctatcaccat
     3361 ttttaatagt aaacgaatct ttagagtaat taatcagtat cacccctagt tcaccacggt
     3421 aatctgaatc aattgtccct ataccattta cgagacctat gccatgcttg gcagctaacc
     3481 cgcttctggc gcgcacctga atttcgtacc cacgtggaat tgccatagca aaaccacacg
     3541 gaatagtagc acggtctaaa tgccccaaaa ctactggctc atcaatggct gcacacacat
     3601 ccatacctgc actatcgtct gtctggtatt gtggtaaaac cgcatctgca cggagctttt
     3661 tgaataatac gttcatcggt tactattgtg ccacacttgc gcgaaattgt aatgcgcaat
     3721 aaagataaat taattatttt gtaagcgtca tttcatatat attaataata cgcttagcgc
     3781 aaatgaatgc tacaaactac gctataaaat taaacgattt taaaggttaa tacccttggt
     3841 gagcgttgcg taatctggaa tttgattttt aaacagcgtc tttacattgc cagcattacc
     3901 agcaaaggta cctgctcttc tgttacatga tgcgctccag ctatttaata ctgggtctgt
     3961 aagatcgttg cctgcaatat aggaatagcg aatacccaaa ggacaatagc ttgtacgatc
     4021 tacgttatct gtattatctc tagtgctgaa gaatcccaca ttctttagcg cactgtagta
     4081 tgcatcatac gacgcttggt tgtttatata gctctctgaa cgcattggac ctacattgta
     4141 gccgttatat atgtctattc ggcgacttgt agcatttact gtaataacaa ttgaataatg
     4201 ctgctctggg ccggtaattt caccatcttg aacgtagcgt acattctgca gctgttcttg
     4261 ctgggcagtt gcttctggtg tcgttggttg ggcaggttca tttgatggct tgcttgggcg
     4321 gaaaagccaa ataattaacc aaataaacaa aaacagcacc aacagtatga gcacgatgcg
     4381 gataattcta ataactcttg gatccatatt tatattatat aacttttata agtatatttc
     4441 aacttcggcg cgtgttttaa ctcttacgct attgatagtt agcatcgact agttcaaaat
     4501 tccgcgttac aaccgcacca agcactgcgt acgttgtatc tgccgtaacg cgtgacgacg
     4561 cactttctac gacagctgcc gtgtttggca tatcacgata aaaagcctgt aattctgatt
     4621 gtaacgtata tactaatgct tctttggtat ctgggctttg taaatgctcc agcataactg
     4681 ggctaatgtc tgttgtaggc ttctgcacca acgcagctat cgcctcatac acagcagtac
     4741 ctacactcgc agcccatgca tgttgcgaat catatagttg ctctatagta atttttacga
     4801 ttggatgccc tactctatac acagcattga ccgattcggc tgtttcatga aaaacgttta
     4861 atacatgtgg attgcttaac ataacctcga gcatctgagc attatcgtct gataaatctt
     4921 gccgaggaaa gtccaactgt ggcgttatgc caccaataaa tgtattcatg gcactagtgt
     4981 acaacgttga tactattgtg caaccataag gggttatcat cgtgctatag ttgatgtata
     5041 tgacttcgca aaaacaacgt tggccaaaat atatttaccg tgtcggcgaa gaaccagacg
     5101 cccgcgtgtc actggcaaac gagcgtactg cactagcagg gctacgcacc gcattggcgc
     5161 tggtagcaac tggcttagga gcggccgcct taaaaaatag tgtttctgtg ccggtggctt
     5221 ttagtgtagc ggcaatcatt ttatctattg ggggtgcagt attggcgatt gtctctgtat
     5281 ttagatgggc aaatgtagaa atagccatac gtacaaaaac accgctgccg gcacccaaag
     5341 gcttgtttac cttagcagtg gttattttga tagttggcat aattagtatt ttagcgataa
     5401 taacatagcg ttatgtggta tctatgaaga aagaaccatc taatgcttct ggtagaaccc
     5461 tttttgcatg gcgacgcaca gcacttgcgt tagtagcaat agcggctctt gctatgaaac
     5521 ttgctggcat tacccagcaa cctattggca tcattacggc aacaatagca ctcttttctg
     5581 cacttgttgt tgcgcaccta agctcaccca aacaaacccc tctgcatagt tcatatgcac
     5641 gcttatggtt gagcgccaca tcagtagtgc tcgtagcaat cagtgctttg gtttgttttt
     5701 ggtaaaaggc tttttgtggc tgcgtaagta cgtactacac ttttctatta atttgtcttc
     5761 cctttttgga ttgtgtgata atttttctaa cgcgtcagtc gtgtcacacc actctttaaa
     5821 atcatgttca gcatccattg ggtccgcctc cgaaacatac tccgcagcat aataatgaat
     5881 gattttttca taatgatgcc catcaagatc gtacgatgca cacacgccac ctaaatatgt
     5941 ctttaatgct acctttaccc ccgcctcttc tgttatctct cgcaaagcac acgcttcaat
     6001 aggctcatca atatgtagcg tacccttagg gagatgatag gtcacctctg cattcgcaga
     6061 agttcttcct aataacaaaa attgcacacc agtattgatg cgtttataca caacaccacc
     6121 ggcgccaata ccatagttat tccgtgctgt atattgtgtt gtgtggctag aaagatgtgt
     6181 catatattct gtatctttat tatagttcac cccagtgctt accggtctta acgtccactt
     6241 tcaggttgat accaagctga ggatgaattg tttccattgt tgatttcata atgtctgcag
     6301 ctataccaat atcgttatca agaacttcta ctaaaatact gtcatgtatt tgcagaagct
     6361 gtttggcggt tttaggcaaa gcgttttcta gtttaatcat ggcgagt
//
LOCUS       DAOPLS010000023         6292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_13285_length_6292_cov_4.236011, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000023 DAOPLS010000000
VERSION     DAOPLS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13285_length_6292_cov_4.236011"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2237
                     /locus_tag="PKV52_01055"
     CDS             <1..2237
                     /locus_tag="PKV52_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HPD98834.1"
                     /translation="FYTQQYATSRVLRSLKNHSMQYVAVLRNNKQVTLSSIDLVPGDI
                     IVVSEGERIPADARIIHTASLQVNESSLTGESVPVHKHASTLATDKQLYEQDNMVFQG
                     TYVLAGMAHALVVETGSRTEFGKIAELATDKQAKSPVQAKIDNLVTLLIKVIGLLAAL
                     VFVLSLARGIPAAESLRFVLSLAVSAVPEGLPVALTVIIVLGMRRMAKQKALVRSFKA
                     IEDIGLITTVATDKTGTLTKNHLKVVDTWSVGSEDTRSVALLTIDQNEKATDPLDIAI
                     SESLDAAHTTKVDTLYPFDLSSRMSGAYIKHSQTLYIKGSPEHVLTLSTLTSEQHRSA
                     ESKMHEFTSKGYRVIAVAMCKLVQAGELKNLAELTKDTLHFVGFIAFADEMRPEAPKA
                     IKAALDAGIQVRLITGDHYETAYNIGNRIGLASHHGQVIQGTDLPKDEQALALAIKDK
                     TIFARILPEDKYRILRALKRTEITAMTGDGVNDVPALANAHVGIAMGSGSDIAKDTGG
                     IVLLDDNFATIIKAISEGRKIFDNIRRMLFYLLSTSLGEVLTMIGALLLGLPLPVTAI
                     QILWINLVTDTAMVLPLGLEPAEDGHMKRPPRKPKDPLLSKLLLSRMALVATTMAIVT
                     IGIVISLEQSGFSTSYIQTVAFMALIVAQWTNAFNARSELNSSFSRFKTVNKGMFVGL
                     MVAVSLQALVMFGPLGYVFDIQEVPLSTLAISSGVMMVAVLGVAELHKLLYKIYLQKK
                     IVAN"
     gene            2288..2521
                     /locus_tag="PKV52_01060"
     CDS             2288..2521
                     /locus_tag="PKV52_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98835.1"
                     /translation="MEVPKPITHQIYDTVFDILEENPQGVRWADLLKEVEKRLPDAHP
                     KTVNGCVWKLVEKYPSRVYKPSKGLFCLVKYKS"
     gene            complement(2588..3814)
                     /locus_tag="PKV52_01065"
     CDS             complement(2588..3814)
                     /locus_tag="PKV52_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPD98836.1"
                     /translation="MSKKKVVFISIVVIILGILGVLTVWLYNNTSLFTTKISNKDLAK
                     NNLNESLNYELVPQKINGKTINIPKGFTLRVFAEDIGSARFLSISPDDTLFVGTKDND
                     KIYTAKDANNDGVADDVKDIDSGLNSPHSVFYYKNDLYVGEENRVSAYRNITSDGTYS
                     KKDVLVDNLPAGNRLTGGGHKTRTVVIGPDEKMYVSIGSSCNVCEENDERRATIMRFN
                     IDGSNGEIFATGLRNTVGFDFKNGQLYGADMGRDQIGDDIPPEEINIIQKNKDYGWPY
                     CYGDNIANPEFPEKTDFCKTKTEKPFANIQAHSAPLGVSFLSQAAQSTWPKQYQDGML
                     VALHGSWNRTIPTGYKVVYIDMSTDEPKQYNFLSGWLENNAEAWGRPVGMVFDSKGNL
                     YLSDDKQGLIYKLSLQ"
     gene            3995..6130
                     /locus_tag="PKV52_01070"
     CDS             3995..6130
                     /locus_tag="PKV52_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD98837.1"
                     /translation="MQPQDEPKKPLETYGQDFTALAKEGALDPVIGRDEEIRRCMQVL
                     SRRNKNNPVLIGEPGVGKTAIVEGLAQRIIKGDVPQSLKDKRLIGLEISNLIAGASYR
                     GQLEERLKNVLQEIEDAAGEIILFIDEIHTIVGAGKAEGAVDIANMLKPMMARGKLHL
                     IGATTLNEYRQYIEKDAALERRLQPVYVGEPNFDDTVAILRGLQEKYEVHHGVKILDE
                     AIIAAARLSDRYITERFLPDKAVDLIDEATSALKMQLDSKPIELDRLNRKIMQLQIEL
                     TSLKKDKSDSAKARKTEIEKDINSYQQKAKLLEDAWQQEKGLIDKLNLASEKLEKLKM
                     QLELAERDGDLALASRIKYGDIPAEEQKLASLRRSLDSIPVEKRLLRDQVMADDIAAV
                     VARWTGIPVDRLKESETKKLSHLENAIQERVVGQDRAVNAVSNAIRRSRAGIGDQNRP
                     IGSFLFLGPTGVGKTELARSLANALFNDERAMTRIDMSEYMEQHAVARLIGSPPGYVG
                     YEQGGQLTEAVRRRPYSILLFDEIEKAHQDVFNVLLQVLDDGRLTDGQGRTVNFSNTV
                     IIMTSNIGSQYIQDWNGKDFASLEATLSNELKKYFRPEFINRVDDIVIFDRIQAEHMK
                     EIVGVQLQRTAEQIKKTKDITLQFTDKAEEALAMQGFDPAYGARPLKRVIQTKILNPL
                     AAKIIEGTVQENDHLLVDVKNGKYTFKTM"
BASE COUNT         1984 a         1212 c         1321 g         1775 t
ORIGIN      
        1 tattctacac gcagcaatat gcgacaagta gagtgttacg tagccttaaa aatcatagta
       61 tgcagtacgt agcagtgttg cgcaataaca agcaagttac tttatcgtcc atagaccttg
      121 tacctggaga tataattgtt gtttctgaag gcgaaaggat tccagcagat gctcggatta
      181 ttcatactgc gagtttacag gtaaatgaat cgtcattaac aggagagtcg gttcctgtac
      241 acaaacatgc atctacactt gcaacagaca aacagcttta cgaacaagac aacatggtgt
      301 ttcagggtac ctacgtactc gcgggcatgg ctcatgctct agttgtagaa acaggttcta
      361 gaactgaatt tggcaaaatt gctgaacttg caacagacaa acaagcaaaa agccctgttc
      421 aagcaaaaat agataatcta gttacacttt taataaaagt gatagggctt ttagctgcat
      481 tagtatttgt gttgtctctg gcacgaggta taccagcagc agaatctcta aggtttgtgt
      541 tatctcttgc tgtttctgcg gtgccagaag gtctccctgt tgcactgaca gttatcatcg
      601 ttctgggtat gaggcgcatg gccaaacaaa aagcgcttgt gagatcgttt aaagccatag
      661 aagatatcgg attaattaca actgttgcaa cagataaaac aggcaccctc acaaaaaatc
      721 acttaaaagt tgtggataca tggtcggtgg gctcagaaga tactcgtagt gtcgctttac
      781 taactataga ccaaaatgaa aaagcaaccg acccactaga tattgcaatt agcgaatcat
      841 tagacgctgc tcacacaact aaagtagata cgctttaccc atttgacctt tctagccgca
      901 tgagtggtgc gtacattaaa cattcgcaaa cactttatat aaaaggatct cctgaacatg
      961 tacttacgct tagcacacta acaagtgaac aacatcgtag cgctgaatct aaaatgcatg
     1021 aatttacttc caaaggttac cgagttattg ccgtggctat gtgcaaattg gttcaagccg
     1081 gcgaactaaa aaacctggca gaacttacta aagatacttt gcactttgta ggctttatag
     1141 catttgcaga cgagatgcgg ccagaggccc ctaaggcgat taaagcagca ctagatgctg
     1201 gtattcaggt taggcttatt acaggcgacc attacgaaac agcctataat attggtaacc
     1261 gtattggcct tgcgagtcac catggccaag tgatacaagg cacagacctg ccaaaggatg
     1321 aacaagctct tgcgcttgct ataaaagata aaacaatttt tgcgcgtata ttgccagaag
     1381 ataaatatag aattttacga gcgcttaaac gtacagaaat taccgccatg acgggtgacg
     1441 gtgtgaatga cgtgcctgcg cttgctaatg ctcatgttgg catagcaatg ggttctggaa
     1501 gtgatatagc aaaagacacc ggtggaattg tgcttctaga tgataatttt gcaacgatta
     1561 taaaggcaat atcagaagga agaaaaatat tcgacaacat acgtcggatg cttttttacc
     1621 tattatctac gtctttaggt gaggtcttaa caatgattgg tgcactcctg ctgggcttac
     1681 ctttgcctgt aacagccata cagattctgt ggataaacct cgttaccgat acagccatgg
     1741 tgttaccgct cgggctagaa ccagctgaag atggtcatat gaagcgacct ccccgcaaac
     1801 ccaaagatcc actattaagt aaattgcttt tgagtagaat ggctttggtc gccacgacaa
     1861 tggcgattgt tacaattggt attgtcatta gccttgaaca aagtgggttt agcacttcat
     1921 atatccagac ggttgcattt atggcgctta ttgtagccca atggacaaat gcttttaatg
     1981 cgcgtagcga attaaattct tcttttagca gatttaaaac tgtgaataaa ggaatgttcg
     2041 ttggtttgat ggttgcggtt agcctgcagg cactggtgat gtttggtcct cttggctacg
     2101 tctttgatat tcaagaagtg cctttatcaa cactcgcaat cagctctggt gtaatgatgg
     2161 ttgcagtact cggtgttgct gaactacaca aacttcttta caaaatatat ttgcagaaaa
     2221 agattgtagc gaactaaacc actacataaa tgcgacaatt acacctagct acgatatact
     2281 gtgtggcatg gaagtaccaa agcctattac gcaccaaatt tacgacaccg tctttgatat
     2341 tttagaagaa aatccacaag gtgtgcggtg ggcagattta ctaaaagaag ttgagaagag
     2401 gcttccagac gcgcacccaa aaacagttaa tggctgcgtc tggaagttgg tagaaaaata
     2461 tccaagccgt gtgtacaaac catcaaaagg cttgttttgc ttagtaaaat ataaatctta
     2521 aaatagggta tttgcaaggt tagtacgaga atcgctacat ctatacaatt ttatttgtta
     2581 cctacaacta ctgcaaagat agcttatata taaggccttg cttgtcatcg ctaagataca
     2641 agttgccctt actgtcaaaa accataccca cagggcgtcc ccacgcttcg gcattatttt
     2701 ctaaccagcc acttaaaaaa ttatattgtt ttggttcatc agtagacata tctatatata
     2761 caactttata accagtaggt attgtacggt tccaactccc atgcagtgca accaacatgc
     2821 catcctgata ttgttttggc catgtagact gagctgcttg gcttaaaaag cttactccaa
     2881 gtggcgcaga gtgagcttgt atgtttgcaa acggtttttc ggtttttgtt ttacaaaaat
     2941 ctgttttttc tggaaactcc gggtttgcaa tattatcgcc ataacagtat ggccacccat
     3001 aatctttatt tttttgtata atatttattt cttcaggcgg aatatcgtct cctatttgat
     3061 cacgacccat gtctgcgccg tacagttgcc catttttaaa atcaaagccg acagtattgc
     3121 gtaaaccagt ggcaaaaatt tcgccgttac taccatctat attaaaacgc attatagttg
     3181 ctctacgttc gtcattttct tcacacacat tacaactact gccaatacta acgtacatct
     3241 tttcatctgg gccaatcaca acggtgcgcg tcttgtgccc tccaccagtt aatcgattac
     3301 cggccggtaa attgtctact aaaacgtctt ttttagagta tgttccgtca gatgttatat
     3361 tgcggtatgc actcacacgg ttttcttcgc caacatatag atcatttttg tagtaaaaaa
     3421 ccgaatgtgg actattgaga ccactgtcta tatctttaac atcatcggcc accccgtcgt
     3481 tatttgcatc tttcgctgta taaattttat cgttgtcttt tgtgcctacg aacaacgtat
     3541 catccggact tatacttaaa aatctagcag aaccaatatc ttcagcaaat actcttaagg
     3601 taaaaccttt tggtatattt atagttttac cattgatttt ttgaggtact aactcataat
     3661 ttaagctttc attaagatta ttcttggcta aatctttatt gctaattttt gtagtaaaca
     3721 aactggtgtt gttgtataac caaacagtta gcacgcccag aatacctaaa ataataacta
     3781 caattgaaat aaacaccact tttttcttgc tcatagtata agtatatcaa tgagcaatac
     3841 ggttacatat aagtaatgac acacaacaaa agctagacta acgtttttgt tggcaattta
     3901 ttacataacg agcgacatac tacgtagtaa attagcactc ttgctatgag agtgctaatt
     3961 tactataatt aagcgtagta aggagatatt tattatgcaa cctcaagatg aacctaagaa
     4021 gccattagaa acctatggtc aagattttac cgcccttgcc aaagagggag cgttagatcc
     4081 ggtgatagga agagacgagg agatacgtcg gtgcatgcaa gtactttcac gtaggaataa
     4141 gaataacccg gttcttatag gtgaaccagg tgtcggtaaa actgcaattg tagaaggact
     4201 cgcccaaaga attattaaag gcgatgtacc gcaatcactt aaagacaagc gactcattgg
     4261 gctagaaata tctaatctta tagctggtgc gagctatcgt ggccagctag aagagcgtct
     4321 taagaatgtt ttacaagaaa tagaagatgc tgctggtgaa atcattttat tcattgacga
     4381 aattcacacc atagtaggtg ccggtaaggc agagggtgca gtagacattg caaatatgct
     4441 aaaacctatg atggctcgtg gtaaattaca tcttattggt gcaacgacgt taaacgaata
     4501 ccgccaatat atagaaaaag atgccgccct agagcgccgt ttacagccgg tgtatgttgg
     4561 cgagcctaat tttgatgaca ctgttgctat tttgcgtggt ttacaagaaa agtacgaggt
     4621 acaccacggt gtgaaaatat tagacgaagc aattattgct gccgcacgtt taagcgatag
     4681 gtatatcact gaacgattct tgccagacaa agcagttgat ttaatagacg aagctacctc
     4741 tgcactaaaa atgcaactag attctaagcc aatagagcta gatagactta atagaaaaat
     4801 tatgcagctg caaatagaat tgacttcttt aaaaaaagat aaaagtgaca gtgcaaaagc
     4861 tcgtaaaacc gagattgaaa aagacatcaa ttcataccaa caaaaagcaa aattattaga
     4921 agacgcgtgg caacaagaaa aaggcttaat agataaatta aatttggcaa gtgaaaaatt
     4981 agaaaaacta aaaatgcagt tagagctggc agagcgtgac ggcgatttag cacttgcgag
     5041 tcgtataaaa tacggggaca taccggcaga agagcaaaag ctcgccagct tgaggcgatc
     5101 actagatagt attccggttg agaagagatt actaagagac caggttatgg cagatgacat
     5161 tgcggctgtg gttgctcgct ggactggtat tcctgtagac agacttaagg aatcagaaac
     5221 aaaaaaactc agccatctag aaaatgctat acaagagcga gttgttggcc aagatagggc
     5281 tgtgaacgca gtttctaatg ctattcgtcg ttctagggcg ggtataggcg atcaaaatag
     5341 accgataggt tcgtttttgt ttttgggtcc aacgggtgta ggcaaaacgg aactcgccag
     5401 gagtttggca aacgccttat ttaatgatga acgtgctatg acgaggatag atatgagtga
     5461 gtacatggag cagcatgctg tggcgcgttt aataggttca cccccaggct atgtcggcta
     5521 cgaacaaggc ggtcaactta cagaagcggt gcgtcgtagg ccgtatagca ttttgttatt
     5581 tgatgaaata gaaaaggcgc atcaggatgt gtttaacgta ctactacagg tactagatga
     5641 tggacgatta actgatggcc aaggcaggac cgttaacttt agcaatacag tcattattat
     5701 gacaagtaac attggttcgc agtatattca ggattggaat ggcaaagact ttgctagtct
     5761 tgaagctact cttagcaatg agctaaaaaa atattttaga cctgagttta ttaaccgggt
     5821 cgacgatatt gttatttttg accgaattca ggcagagcac atgaaagaga ttgtgggtgt
     5881 acagttacaa cgcactgcag agcagattaa aaaaaccaaa gatattaccc tacagtttac
     5941 cgataaagca gaggaagcgc tggctatgca aggctttgac ccagcatatg gtgcacgccc
     6001 attaaaacga gtcattcaga cgaagatatt aaacccgttg gcggctaaaa taattgaagg
     6061 taccgtacaa gagaacgatc atctacttgt tgatgtgaaa aacggaaaat atacttttaa
     6121 aactatgtaa gatctttact acatagatta aaaaagtaca aaatatccca tacgttgttt
     6181 atttggcgtt ctaagtcgat acttattttg aagtttttta gcagcatgct gtagtatctt
     6241 tgctatgtgc ggcgtggcat aaaaaacggc tattgcgagc attgcaccaa gt
//
LOCUS       DAOPLS010000024         6145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_13724_length_6145_cov_4.728079, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000024 DAOPLS010000000
VERSION     DAOPLS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13724_length_6145_cov_4.728079"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /gene="tilS"
                     /locus_tag="PKV52_01075"
     CDS             <1..787
                     /gene="tilS"
                     /locus_tag="PKV52_01075"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPD98838.1"
                     /translation="HGIRKDSGVDAMFVEELANNSGYTYETTQLSLGNDTSEDLARQK
                     RYEFLYRMQKKHQAAAIITAHHQDDMIETMFLNIMRGTKRKGIASLQSTDTIIRPILY
                     VPKRDIYNYAKAHNLQWREDSTNIDTAYARNNIRHTVVAKMNTQQRAQALAIIQNTQL
                     LNTKIDKELRLLITRGLHKDTLVLNRSWFCKLPHDISTEVVHMLLRRANAANIDTPTI
                     EKVTVAIKTMLPGKVIQVSGVDIQLTKRSARFISRSQTDEKTV"
     gene            797..2662
                     /gene="ftsH"
                     /locus_tag="PKV52_01080"
     CDS             797..2662
                     /gene="ftsH"
                     /locus_tag="PKV52_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009628744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPD98839.1"
                     /translation="MDKKKVPQKKPDNKKRLYKNLGFAVIVALFVGVFLLSFNKPAEL
                     AQKPYNDVMNRANRGEISKLVVSGEKIEVTLNDQDKASEVTRGEAGSSIYGPGGLTNR
                     DVSVEFKEASSSGATITALLINLLPVIIIVGVLIFLLRSAQGQGNQALGFGKSSARLY
                     GNEKEVVKFNDVAGNDEAKVDLQEVVEFLKFPKKFQAVGARIPKGVLLVGPPGTGKTM
                     MARAVAGEANVPFFSISGSEFVEMFVGVGASRVRDLFSKAKKNAPCIIFVDEIDAVGR
                     RRGSGMGGGHDEREQTLNQILVEMDGFEQGQNVIVLAATNRMDVLDPALLRPGRFDRR
                     VAIGLPDRKDRLEILKVHFSKKPLQKGVDLESLAAKTAGSSGADLANIANEAAIVAAR
                     QDRTKITEQDITEAFEKVAIGPERKSKVMSEKEKELTAYHEAGHAIVGHVLPDSDMVH
                     KVTIIPRGGTGGVTWFIPPEDKSYHSIIEYKDVLARMLGGRIAEEVIYGTERVTTGAG
                     NDLQKAAELARDMVVNQGMGKQLRDQVFHVEGGMMLDRLVSERQYSDDTAKIIDDEVE
                     ALITEAAHRARVVIRANKQYLDSLKDALLEKETVEAEEVIKLLSGSTMPSAAALY"
     gene            2702..4273
                     /locus_tag="PKV52_01085"
     CDS             2702..4273
                     /locus_tag="PKV52_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPD98840.1"
                     /translation="MNRFLNRANKRISLGTAAALLAGTSFLGIFLGILRTKLINANFN
                     VFTSDAYFAAFKIPDFIFFTLASGALGVAFLPILSDRLAKNRQSAWEVSSYILNFLGL
                     IALVASILLMLLADPLMRYVVAPGFTPQQLELSVSIMRIVSVNVFIFSISTILSTVQQ
                     AVGRFFFIAIAPLFYNTSIIASIFIFGDSLGIVGLSLGVALGSILQLLVVALGMAGLK
                     FKYTPLIDFKNQSFREVLRVLPARAIDQGVDSINAIVETRFASRLLTGSVTWYENALV
                     LHFAPISLIGNSISTAAFPRLTDRLAQGRTDLFRKEFIKVLRTMIWIALPVVVVSFFA
                     RAYLARIIFARSSSEIAMIFGFLSVAIFFRIIYTIISRYFYAHKDTKTPLYVSLFVIA
                     LNVFLAYTLSKPSAYGVAGLAIAQSIVALTEVMILVAIMIQRDRKLFTGDFVQAITRM
                     IAITGFSAFATYVAIQFFPLLQTDTGFVLMIKLGIITSITFTVHTAMSYVFDMPEARA
                     VVQKVRTMALAVIKV"
     gene            4270..6051
                     /gene="lepA"
                     /locus_tag="PKV52_01090"
     CDS             4270..6051
                     /gene="lepA"
                     /locus_tag="PKV52_01090"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPD98841.1"
                     /translation="MNVEKIRNFCIIAHIDHGKSTLADRLMELTNTVDKRNMKAQLLD
                     KMDLEREKGITIKLAPVRMVYDNHELNLIDTPGHVDFSYEVSRSLEACEGAILVVDAS
                     QGIQAQTLANVYLAMAADLTIIPVLNKIDLPAADTEKVSAEVIALLGCTKEDILHISA
                     KTGKGVETVLDRIIADVPPPQGSADQPTRALIFDSYYDDYRGVILYVRVFDGAIQKND
                     AIHMIGTRSLGIALEVGCLQPGMIVTNTLDTGEIGYIVTNLKSTREARVGDTVTLEKT
                     QAHEALPGYKEVKPFVYAGFFPDSNENYQLLKDAIEKLSLSDSALQFTPENSPVLGFG
                     VRIGFLGLLHMDIIKERLEREYGLELVITNPSTDYHVTLITGETLDIKSASELPDPAK
                     IETIKEPWIKGEVVCPKEYVGSVIQLIVSKRGSYKNVSYVDATLALISFDAPLANLLT
                     DFYDQLKSITSGYGSFNYELHNYRAEDLVRLDFYVAGERVDALGIMVHRSEAEHLGRV
                     TVKKLKDVIPRQNFQVALQAAIGGRFIAREDISAYRKDVTTGLYGGDVSRKKKVLAKQ
                     AKGKKRMKRFGKVDIPAEAFTVMLKRD"
BASE COUNT         1861 a         1154 c         1380 g         1750 t
ORIGIN      
        1 ccacggtata cgaaaagact ctggcgtaga tgctatgttt gttgaagagc tagcaaacaa
       61 tagtggctat acatacgaaa caacgcagtt gtctttaggc aatgatacca gcgaggattt
      121 ggcccgccaa aaacgttatg aattcttata tagaatgcaa aaaaaacatc aagctgctgc
      181 aattattact gctcatcacc aggacgatat gattgaaaca atgtttctta acattatgcg
      241 aggcacaaaa cgcaaaggta ttgcttcgtt gcaaagtacc gatacgatta ttcggccaat
      301 actttatgtt cctaagcgtg atatctacaa ttacgccaaa gctcataatt tgcagtggcg
      361 tgaagacagt acaaatattg atacagcata cgccagaaat aacattaggc atactgtcgt
      421 tgccaagatg aatacccagc aacgcgctca ggcactggct ataattcaaa acacgcaatt
      481 attaaacaca aaaattgaca aagagctccg gctacttatt acccgcgggc tacataaaga
      541 tacactggtg ttaaatagaa gctggttttg caaattgccc cacgatatct ccacagaagt
      601 agtgcatatg ttgctacgaa gagctaacgc ggcaaacatt gatactccca ctattgaaaa
      661 agtaaccgtt gctatcaaaa cgatgttgcc cggaaaagtg attcaggtgt ctggtgttga
      721 tattcaatta actaaacgtt ctgccagatt tatttcacgt agtcaaacag atgaaaaaac
      781 ggtataattg attgatatgg ataaaaagaa agtacctcag aaaaaacctg ataataaaaa
      841 aaggttgtat aagaacctag gttttgcagt tattgttgcg ctattcgttg gcgtattttt
      901 gcttagtttt aataaaccag ccgaactcgc tcaaaagcct tataacgatg taatgaatcg
      961 tgctaatagg ggtgaaatat ctaaacttgt tgtctctggc gaaaaaatag aagtaacgct
     1021 caatgatcaa gacaaagcca gtgaggttac ccgtggcgaa gcaggttcga gtatttatgg
     1081 gccaggaggc ttaacaaata gagatgtatc tgttgagttt aaagaagctt cgtctagtgg
     1141 cgcaacaatt actgcactgc tcattaattt attaccagta attatcattg taggtgtgct
     1201 tatttttctt ttaagatctg cgcaaggaca agggaaccaa gcgcttggtt ttggcaagag
     1261 cagtgctcgg ttgtatggta atgaaaaaga agttgttaaa tttaacgatg ttgccggcaa
     1321 cgacgaagca aaggtagacc tccaagaagt ggtagaattc ttaaaattcc ctaagaaatt
     1381 ccaagcagtt ggcgctcgta ttcctaaggg tgtattacta gtaggccccc ctggaacagg
     1441 taaaactatg atggctcgag cggttgctgg tgaagcaaat gtacccttct ttagcatatc
     1501 tggttcagaa tttgttgaaa tgtttgttgg tgttggtgct tcccgagttc gtgatctttt
     1561 tagtaaagct aaaaagaatg ccccttgtat aatttttgta gacgaaatag acgccgttgg
     1621 ccgccgccga ggcagtggca tgggcggagg tcacgacgaa cgtgagcaaa cactaaatca
     1681 aatacttgta gaaatggatg gctttgaaca aggccaaaac gttattgtat tagccgctac
     1741 aaaccgcatg gatgtgcttg atcctgcttt gttgcgccct ggacgatttg acagacgggt
     1801 ggccatagga ttacccgacc gtaaagatag gttagaaata ctaaaagtac atttttctaa
     1861 aaagccgcta caaaaaggtg ttgatctaga atctttggca gctaaaacgg ccggctctag
     1921 cggggcagat cttgcgaaca ttgcgaacga agctgctatt gtagctgccc gccaagatcg
     1981 taccaagatt actgaacaag acattactga agcgtttgag aaagttgcaa taggaccaga
     2041 acgtaaaagt aaggtaatga gtgaaaaaga gaaagaactt accgcctacc atgaggctgg
     2101 acacgctatt gttgggcatg tattgcctga tagcgacatg gtccataaag taacaattat
     2161 accgcgaggt ggcacaggtg gtgttacgtg gtttatcccg ccagaagaca aaagctatca
     2221 cagtatcatt gaatacaaag atgttttggc gcgtatgcta ggtggccgta ttgccgaaga
     2281 agtaatttat ggtacagaac gtgtcactac tggcgctggt aatgatttac aaaaagctgc
     2341 cgaacttgct cgcgacatgg tagtcaatca aggtatgggt aagcagttac gtgaccaagt
     2401 gtttcacgtt gaaggcggta tgatgcttga taggctggta tctgaacgtc agtattcaga
     2461 tgatacggca aaaatcattg acgatgaggt agaagcactc atcacagaag ccgcccaccg
     2521 tgcgcgggta gtaatacgtg cgaataagca atacctagat tctttaaaag atgcattgct
     2581 agaaaaagaa actgtagaag cagaagaagt aataaagcta ttatcaggat ctacgatgcc
     2641 gtcagctgcg gccctttact aggggttctg tcgccgacat agcgcataca aaactcgtcg
     2701 tatgaaccga tttttaaaca gagccaacaa acggattagc ctcggcacag cagcagcgct
     2761 cttggcgggt acgtcattct taggaatatt tttaggtata ctgcgcacta aattaattaa
     2821 tgcgaacttc aatgttttta cttctgacgc atattttgct gcatttaaaa tcccagattt
     2881 tatctttttt actcttgcat cgggtgctct tggagtcgct tttttaccga tattaagtga
     2941 tagactcgct aaaaatagac aatctgcatg ggaagtctca agttatattc ttaacttttt
     3001 gggtttaata gcacttgtgg cttctatttt gttgatgttg cttgccgacc ctctgatgcg
     3061 ttacgttgtg gcaccagggt ttacaccaca acagcttgaa ctatcggttt ctattatgcg
     3121 cattgtttct gttaacgtgt ttatattttc tatttctacg atattatcta cggtgcagca
     3181 agccgttggg cgttttttct ttatagccat tgcaccattg ttttataaca cttctattat
     3241 tgccagtata tttatctttg gtgactcgct cggtatcgtt gggctgagtc ttggtgtcgc
     3301 gcttggttct attttgcaat tacttgtagt ggcgcttggt atggctggtc ttaaatttaa
     3361 atacactcca ttaatagatt ttaaaaacca aagctttaga gaagtgctac gggtgcttcc
     3421 agctcgtgct atcgaccaag gtgtagatag tatcaatgca attgtagaaa cgaggtttgc
     3481 ttctcggttg ctgactggat cggttacttg gtatgaaaat gctctggtat tgcactttgc
     3541 gccaatttca ttaattggaa actctatatc gactgctgcg ttcccccgac taacagatag
     3601 gctagcgcaa gggagaactg atctttttag aaaagaattt attaaagtac taagaacaat
     3661 gatctggata gctctgccag tcgttgttgt tagctttttt gctcgggcat acttggctag
     3721 aattattttt gcacgaagca gtagtgaaat tgctatgatt tttggatttt tgagtgtggc
     3781 aatatttttt agaataatat acacaataat ttctcgctat ttttatgctc ataaagacac
     3841 caagactccg ctatatgtat cactttttgt gatagcactg aacgtatttt tagcgtatac
     3901 actatctaaa ccgagtgcct atggagttgc cggtttagca attgcccaaa gtattgttgc
     3961 gctcacagag gttatgattt tagttgcaat catgattcaa agagatagaa aattatttac
     4021 aggagatttt gttcaagcga ttactcgtat gatagctatt acaggattta gcgcttttgc
     4081 tacatatgta gcaatccaat tctttccact actacaaacg gacacaggtt ttgtgcttat
     4141 gataaaacta ggcattatta cttcaatcac gtttacggtg cacacagcca tgtcgtatgt
     4201 ttttgatatg ccagaagcgc gtgcagttgt acaaaaagta agaacgatgg cattagccgt
     4261 cataaaggta taaacgtgga aaagattaga aatttttgta ttattgccca tattgatcac
     4321 ggtaaaagca ctttagccga taggctcatg gaacttacca atacagtaga taagcgcaac
     4381 atgaaggcgc agctcttaga taaaatggat ctagaacgcg aaaagggaat aaccattaaa
     4441 ttggcgcctg tacgcatggt gtacgataat catgagttaa atcttataga cacaccaggg
     4501 catgtagact ttagttacga ggtgtctcgt agtctagagg cttgtgaagg tgcaattcta
     4561 gtggtagatg ccagccaggg tattcaagcc cagacgttag caaatgtgta tttagcgatg
     4621 gctgcagacc tgacgattat ccccgtactt aataagattg atctgccagc cgcagataca
     4681 gaaaaagtta gcgcagaagt cattgcacta ttaggctgta caaaagaaga tatattacat
     4741 atttctgcta agacgggcaa aggagtagaa acagtacttg atcgtattat tgcagatgtg
     4801 ccgccaccac aaggctcggc agaccaacca acccgcgcat taatctttga tagctattac
     4861 gatgattatc ggggggttat tttatatgtg cgtgtttttg acggagcaat ccaaaaaaac
     4921 gatgcaatac acatgattgg tacccgcagc ttgggaatcg cccttgaagt aggttgtttg
     4981 cagcctggca tgattgtaac taacacatta gacactggtg aaatcggcta cattgtcact
     5041 aaccttaaaa gtacccgcga agcaagggta ggagatacgg taacgttaga aaaaacacaa
     5101 gcacatgaag cattgcccgg ttacaaagaa gtaaaaccat ttgtttatgc gggatttttt
     5161 ccagacagta acgaaaatta tcaattgcta aaagacgcca ttgaaaagct cagcttaagt
     5221 gattcggcat tacaatttac accagaaaat tcaccagtgc ttggctttgg cgttcgtatt
     5281 ggctttttgg ggttactcca tatggatatc attaaagaga ggctcgaacg agagtacggg
     5341 ctggaacttg taattaccaa cccaagcact gactaccatg taactcttat tactggtgaa
     5401 acccttgata ttaaaagcgc cagcgaactg cctgatccgg caaaaataga gactataaaa
     5461 gagccctgga taaagggtga agtcgtgtgt ccaaaagagt acgtaggctc tgtcatacag
     5521 ctcattgtca gcaaacgcgg tagctataaa aatgtgagtt atgttgatgc tacactagct
     5581 ttaattagtt ttgatgctcc actcgcaaac ttactgacag atttttacga tcagcttaaa
     5641 agtatcacga gtggctacgg atcatttaat tatgagcttc ataattacag ggcagaagat
     5701 ttagtgcgac ttgactttta cgttgctggt gaacgcgtag atgcgctagg aataatggta
     5761 caccgcagtg aggcagagca tctaggccgc gtaacagtaa aaaaacttaa agatgttata
     5821 ccacgtcaaa attttcaagt agcgttgcaa gcagcgatag gtgggcgatt tattgcaaga
     5881 gaagacatta gtgcgtaccg taaagatgtt actacggggc tatatggtgg ggatgtatct
     5941 cgtaagaaaa aggttctcgc gaagcaggct aaaggtaaaa aacgtatgaa acgtttcggc
     6001 aaagttgata tcccggcaga agcattcact gttatgctta agcgtgatta actgcttgta
     6061 catcacgcaa tacttcccct atatgggtag caggatttac attttcataa gtttttacta
     6121 ttgtaccatc gggactaatg agata
//
LOCUS       DAOPLS010000025         6071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_13964_length_6071_cov_3.482879, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000025 DAOPLS010000000
VERSION     DAOPLS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13964_length_6071_cov_3.482879"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PKV52_01095"
     CDS             complement(<1..970)
                     /locus_tag="PKV52_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD98842.1"
                     /translation="MQRIQRKIGNHPLLAVFATVFVDLVGVGILIPIFPLLVTPGSPD
                     SILPNGWTITQGVILLGWLSAAYPLAQFIAAPILGQLSDKYGRKIVLTLSIIGTALGY
                     VLFAIGLATKNIPLLFASRILDGFTGGNISVAQAAIADISTAKNRAKNFGLIGMAFGL
                     GFILGPYIGGKLADPSVMSWFNATTPFWFAAGLSVFNVILITLFLPETLKVRKTARID
                     FSRPFHNVVTAFSRPGLRSVMPTSFLFTAGFTFFTTFFGVVLAQKFNFSVSNTGDYFA
                     YVGLWIAIAQGVFVGIVAKRFKDYQVLRVSVFITAVSLLSYAMIPAG"
     gene            complement(1014..1805)
                     /gene="rsmA"
                     /locus_tag="PKV52_01100"
     CDS             complement(1014..1805)
                     /gene="rsmA"
                     /locus_tag="PKV52_01100"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPD98843.1"
                     /translation="MVVDSRPNKQLGQHWLTDNETLSNIVAAADISAADTVLEVGPGL
                     GTLTQALLATGASLTAVEYDAALATQLMKNIHEPNVQVIEHDILTFDFRSLPKDYKVV
                     ANIPYYLTSKLVRIVCESANPPKTTVLLVQKEVAQRIAAQPGAMSLLSISAQVYNTTT
                     LGVVVPAALFTPPPKVDSQVIIMQRRPLPLVPHSLQKTFFRVVKAGFNERRKKLRSSL
                     SGGLHLEKQQVDELLASVSINASARAQELSIDDWVRLTKKYIANQ"
     gene            complement(1796..2989)
                     /locus_tag="PKV52_01105"
     CDS             complement(1796..2989)
                     /locus_tag="PKV52_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HPD98844.1"
                     /translation="MNIHTSVTSFFHKHAFALGIFGILFGFTFLSSSFVVANGQTIGP
                     EDSHIVSLYINGEETVVPTRAKTVGELLTKTDIALTESDLVEPAKDTAIDNDNFKVNV
                     YKARPVSIIDAGKVVRAFTPYQGAKLVAEKAGVKVYPEDTYTLESGDSFVDEQIIGEK
                     ITIHRATPVTINLYGSGPVTYRTHVKTIGDLLAERNITPEEDATLSPAADTPLTPNLA
                     IFISKFGKQVVTTEESIPFETATTPDPSAPLGRITVTSPGKPGVKKVTYEIETRDGRE
                     VGRVVLQEVRVTEPTTQTQTKGTKANVSGDKLEWMRAAGIAESDFGAVDFIIGHESGW
                     RPAAMSGSGCGGLGQACPSSKLARACPDWQVDPVCQLRFFSGYSGRYGGWQGAYQAWL
                     VKGWW"
     gene            complement(3098..3559)
                     /locus_tag="PKV52_01110"
     CDS             complement(3098..3559)
                     /locus_tag="PKV52_01110"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD98845.1"
                     /translation="MKHSRALGKITYICTYPFIRIWLYGSHRAYVILVVKDEVLLTKN
                     WLGLHQRWRLPGGGVHASESVLDGLVREISEEIGCSLPIKKVHTLGQQRYYKASKLYT
                     YSLYTVKLNKKPRLHINHNEIADHAWFPIAKAKTQPLAEETRHALQLLDIA"
     gene            complement(3556..5697)
                     /locus_tag="PKV52_01115"
     CDS             complement(3556..5697)
                     /locus_tag="PKV52_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPD98846.1"
                     /translation="MSQFTLGLNAEQQRAVESTDGPLLILAGAGSGKTKTLTHRIAHL
                     LDTQLATQYEILAVTFTNKAAKEMRERVAQLLAYRRDDKAFMPYMGTFHGICVRILRK
                     DGQYSGVPNNFVIFDESDRTTLIKQLSKQLSIDEKQHTARAIGAIISAAKNDLITSAE
                     YASLAQTPTQRAAARVYPLYQQALKDHAALDFDDLIGKVVSLLENNAEVRARWQDRFK
                     YILIDEYQDTNTAQYKLIKLLLNEKRNICVVGDDWQSIYSWRGADFKNILNFERDFKN
                     ATVIRLEQNYRSTNHILDAAHAVIAKNAQRSDKKLWTAISGGAPVQVLSAYNERHEAE
                     QIIQIIRQRTDTRTFAYKNIALLYRTNAQSRALEEQFVRFNIPYKMVGGARFYDRTEI
                     KDILAYLRLLYQPEDIISFQRIVNTPTRGFGKTSLDKFLQWRQVQGLTLTDALDNVDT
                     SPLTARAKQALFALHELLSVYRQEMDHLSAASLLESVIKRLDYYNYLDDGTLQSEARI
                     ENVKELLSVAKAYSDEGLASFLEEVALVSDLDTLNPNSDAVTLMTLHAAKGLEYDVVF
                     MTGMEESIFPHSRALFDQSQMEEERRLCYVGMTRAKKELYLTYADSRVLYGGVQHYVP
                     SRFIAEINAQHIKKAAYTGYASAGVLDNNQPESSEPRYVPELHPGDTVNHQVFGRGIV
                     VDLDGEVAAVQFGGKGIKRLNISFAPLEKVS"
     gene            complement(5742..>6071)
                     /locus_tag="PKV52_01120"
     CDS             complement(5742..>6071)
                     /locus_tag="PKV52_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98847.1"
                     /translation="SATTPEPTVTNENPPEAPNEPVEHAQEQKSSISPTAYEASDSLP
                     DMSEKRTTELTDEMQTPRIYDTKEYIVPIHDTLHSHGTTGKIVAGIVSGLVVLIIVIV
                     AAYAIGG"
BASE COUNT         1642 a         1329 c         1256 g         1844 t
ORIGIN      
        1 cacctgctgg tatcatggcg tagctcagca aacttactgc agttataaat acacttactc
       61 ttagtacttg gtagtctttg aagcgttttg caacaatacc aacaaaaact ccttgagcga
      121 tggcaatcca taacccaaca taagcaaaat aatcacctgt attactgacc gaaaagttaa
      181 atttctgggc aagtacaaca ccaaaaaacg tggtaaaaaa ggtaaacccg gcagtaaaca
      241 aaaagcttgt tggcataaca ctgcgcaaac ctgggcggga aaaggccgtc actacattat
      301 gaaacggccg tgaaaagtct atacgtgcgg ttttacgtac ttttaatgtt tctggcaaaa
      361 atagtgtaat taatatcacg ttaaagacac ttaaaccggc cgcgaaccaa aatggtgtag
      421 ttgcattaaa ccagctcata acagatgggt cggctaattt tccaccaatg tatggaccta
      481 atataaagcc tagtccaaac gccatgccaa ttaagccaaa gtttttagcc cgatttttag
      541 cggtactaat atcggcaatg gcagcttgag caactgatat attaccaccc gtaaagccgt
      601 ctaggatgcg cgatgcaaat aacagtggaa tatttttggt tgctaagcct atggcaaaga
      661 gtacataccc aagagctgtc ccaataatag acagcgtaag aacaattttg cggccatatt
      721 tatctgataa ttgccctaag ataggtgctg caataaactg tgcgagtgga tatgccgcac
      781 tcaaccagcc tagtaaaata actccttggg taatcgtcca accatttggc aaaatagaat
      841 ctggcgaacc tggcgtcacc aacagcggaa aaatcggtat tagtatacca acaccaacta
      901 aatcaacaaa taccgtggca aacacggcga gcagtggatg gttaccaatt tttctttgta
      961 tacgttgcat gagacttata actttaaaat atatagttag tatacgacag tttttactga
     1021 ttcgctatat attttttggt aagacgtacc caatcatcta tagaaagttc ttgcgcacgt
     1081 gccgacgcgt ttatagatac actggccagt agttcgtcta cctgctgctt ttctaaatgt
     1141 aagccaccac tgaggctgct gcgtagtttt tttcggcgtt cattaaagcc agccttaacg
     1201 acccgaaaaa aggttttttg aagactgtgt ggcactaatg gtaatggcct acgctgcata
     1261 ataattacct gtgaatctac ttttggtggt ggcgtaaaaa gcgcagctgg tacaacaaca
     1321 cctagagttg ttgtgttata gacttgcgcg ctgatactta gcagcgacat agcaccgggt
     1381 tgagcagcga tacgctgtgc gacttctttt tgaacaagca acacggtggt ttttggtgga
     1441 ttagcagatt cgcatacaat acgcacaagt ttagacgtta aataatacgg aatatttgca
     1501 accactttgt aatcttttgg taatgaccta aagtcaaacg ttaatatgtc atgctcgata
     1561 acctgtacgt taggctcgtg aatatttttc atgagctgtg tggcgagtgc tgcatcatat
     1621 tccactgctg tcaggctcgc accagtggct aataatgctt gggtgagcgt accaagacca
     1681 ggaccaacct cgagaactgt atcagcagcg ctaatatcgg ctgccgccac aatattcgat
     1741 aatgtttcgt tatctgtaag ccaatgttgg ccgagctgtt tatttggccg tgaatctacc
     1801 accatccttt tactaaccac gcctggtagg caccttgcca gccaccataa cggccagaat
     1861 atccactaaa gaatcgtaat tgacatactg ggtcaacttg ccaatcaggg cacgctctgg
     1921 cgagtttaga cgatggacac gcttggccaa ggccaccgca cccactacca ctcattgcag
     1981 ctggtcgcca gccagattca tgacctatga taaaatcaac agcaccaaaa tcagactctg
     2041 caataccagc tgctcgcatc cattcgagct tatcgccact aacattggct ttggtacctt
     2101 tggtttgtgt ctgcgttgta ggttcggtaa cacgaacttc ttgcaacact actctgccaa
     2161 cttcacggcc atctcttgtt tctatttcat aggttacctt tttaacacca ggcttacctg
     2221 ggctggtaac ggtaattctc ccaagtggtg cgcttggatc tggagttgtt gctgtttcaa
     2281 aaggtattga ctcttctgtt gtaacaacct gcttcccaaa cttagaaata aatattgcta
     2341 aatttggagt aagaggtgtg tctgctgccg gcgacaatgt agcgtcttct tctggcgtga
     2401 tattacgttc tgctaacaga tcaccaattg tctttacgtg ggtgcgatac gtcaccggtc
     2461 cagaaccata caagttaatc gtcactggtg ttgcccgatg aatggttatc ttttcgccga
     2521 taatttgttc gtccacaaac gaatcgcctg attcaagtgt gtaggtatct tctgggtaca
     2581 cttttacacc agctttttcg gcgacgagtt ttgcgccctg atatggcgta aatgcacgca
     2641 ctactttgcc agcatctata atgcttacag ggcgcgcttt atatacgttt accttaaaat
     2701 tatcattatc tatagctgtg tctttagcag gttctacaag gtcgctttct gtaagggcta
     2761 tatctgtttt tgtgagcagc tcgcctaccg tttttgcacg tgtgggtacc acggtttctt
     2821 caccgttgat atatagactt acaatatggc tgtcttctgg ccctattgtc tggccattgg
     2881 ccacaacaaa actgctcgat aaaaaggtaa atccaaaaag aataccaaag atacctaagg
     2941 caaaagcgtg tttatgaaaa aatgatgtta cagatgtatg tatattcatg aacaaaaacc
     3001 tctactttgg agcaaatgcg ttattggtat aaacaaatgt ttacttctta acgttcggtt
     3061 ctcaaaataa aggtactgaa catcattata gcatatttta ggctatgtct agcagctgta
     3121 aggcgtggcg cgtttcttct gctaatggct gtgttttcgc ttttgctata gggaaccatg
     3181 catggtcagc gatttcatta tggttgatat gcaagcgagg ttttttattc agttttaccg
     3241 tgtataaact atatgtgtag agtttgcttg ctttgtagta gcgctgttgc cccagagtgt
     3301 gcaccttttt aataggtaaa ctgcagccaa tttcttcgct tatttctcta actaggccat
     3361 caagcactga ttctgatgca tgcacaccac cgcctggtaa tcgccacctt tggtgtaggc
     3421 caagccagtt ttttgttagt aaaacttcgt cttttactac tagtattaca tacgcacgat
     3481 gtgagccata tagccagata cgtataaacg gataggtaca aatataggta atttttccta
     3541 acgcgcggga atgcttcatg ataccttttc tagaggggca aaactaatat ttaaccgctt
     3601 aatgcccttg ccaccaaact ggactgcagc tacttcacca tctagatcta ccacgattcc
     3661 cctcccaaac acttggtgat taactgtgtc gcctgggtgt aattctggca catatcgtgg
     3721 ctctgatgat tcgggctgat tattgtctaa aactcctgcc gatgcatagc ctgtgtaggc
     3781 agctttttta atatgctgtg cgttaatttc ggctataaac ctactgggga cgtaatgttg
     3841 aacaccacca tacagaaccc tactatctgc ataggtaaga tatagttctt tttttgcacg
     3901 cgtcatacca acgtaacaga gccggcgctc ttcttccatt tgtgattgat caaacaaagc
     3961 ccgtgaatgt ggaaaaattg actcttccat gcccgtcata aatactacgt catattctag
     4021 acctttcgcg gcgtgaagtg tcattaatgt cacagcatct gaattagggt taagcgtatc
     4081 tagatcgctc acgagtgcaa cttcttctaa aaagcttgca agcccttcat cactgtaggc
     4141 tttggccaca cttaatagct cttttacatt ttctatacgg gcttctgatt gcaacgtacc
     4201 atcgtctaaa taattgtaat agtctaaacg cttaatgaca ctttctaaaa ggctcgctgc
     4261 agataggtga tccatttctt gccgatagac acttagcagt tcatgtagcg caaataatgc
     4321 ttgtttagcc cgagctgtga gtggtgacgt atctacatta tcgagagcat ctgtaagtgt
     4381 aagcccttgt acttggcgcc actgtaaaaa tttatctaat gatgtcttac caaaacctct
     4441 tgtaggtgtg ttcacgatac gctgaaaact aataatatct tctggttgat acagtagtcg
     4501 taaatacgca agaatatctt tgatttcggt tctgtcataa aaacgagccc cgccaaccat
     4561 cttatagggt atgttaaagc gaacaaattg ctcttctaga gcacggctct gagcatttgt
     4621 tctatagagt agtgcaatat ttttgtaggc aaaagtacgg gtatcggtgc gttgtctaat
     4681 aatctgtata atttgttcgg cttcgtgccg ttcgttgtat gcactgagta cctgcaccgg
     4741 tgcaccacca cttatggctg tccagagttt tttgtctgaa cgctgtgcgt ttttagctat
     4801 aacagcatgc gcggcatcta gaatatggtt cgtactacga taattttgct cgagacgaat
     4861 aacggtagcg tttttaaaat ctcgttcaaa atttaatatg tttttaaaat cggcaccacg
     4921 ccaactataa atactttgcc aatcatcgcc taccacacaa atattacgtt tttcatttaa
     4981 aagcagctta atcaatttgt actgggcggt gttggtgtct tgatactcat caataagaat
     5041 gtatttaaat ctatcttgcc atcttgctcg cacttcggca ttattttcta gtaaactcac
     5101 taccttacct ataagatcat caaaatctaa ggcagcgtga tctttaagag cttgttgata
     5161 caacgggtac acgcgagctg ctgctcgttg agtaggtgtt tgtgctaatg aagcgtattc
     5221 agctgacgtt attaaatcat ttttggcagc actaataatc gcccctatgg cccgcgcagt
     5281 atgttgtttt tcgtctatac taagctgttt agataattgt ttaatgagtg ttgtacgatc
     5341 actctcgtca aaaataacaa aattgttagg tacgccacta tattggccat ctttacgtaa
     5401 tatccgcaca catatcccat gaaacgtgcc catgtagggc ataaaagctt tgtcgtcacg
     5461 acggtacgca agcaactgag cgacacgttc acgcatttct ttggcagctt tatttgtaaa
     5521 cgtcacggcg agtatttcgt attgagttgc cagttgtgtg tctagtaaat gagcaattcg
     5581 gtgtgtcagc gttttggttt taccacttcc agcacctgcc agaataagca atggaccatc
     5641 ggttgattct acggcacgct gttgctcagc atttaagcct aaagtgaact gtgacattat
     5701 tatattttag caacctaagg tgtgcataag tgttgtgtaa tctatccacc tatagcgtat
     5761 gccgcaacaa ttactataat aagcacaaca agaccactta caatacccgc tacaatttta
     5821 cctgtcgttc cgtgactgtg cagagtatcg tgtattggga caatatattc ttttgtatcg
     5881 taaattcggg gtgtttgcat ctcgtctgtt aactcggtgg tacgtttttc tgacatatct
     5941 ggcaacgaat cagacgcctc gtaggcggtc gggcttatac tactcttttg ttcttgggcg
     6001 tgttctacgg gttcatttgg cgcctccggc ggattttcat ttgtaaccgt tggttcgggc
     6061 gtggttgcgc t
//
LOCUS       DAOPLS010000026         6007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_14171_length_6007_cov_5.192708, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000026 DAOPLS010000000
VERSION     DAOPLS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14171_length_6007_cov_5.192708"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2988
                     /locus_tag="PKV52_01125"
     CDS             <1..2988
                     /locus_tag="PKV52_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPD98848.1"
                     /translation="DRRRKIPVTTLLKALGMSETAIKEAVKHVNTGTTDYVAATIEKD
                     PSKGAQEALIEVYRRLRPGDLATIDNARSLIESMFYTYKRFDFSRVGRYKINQRLDLD
                     VENTLENRVMRLDDMLAIVSEIIRLNNTQEPADDIDSLANRRIKLVGELVQRQFRIGL
                     LRMDRNAKDRMSMSEIETVSPSQLVNARPIVAAVREFFASSQLSQFMDQINPLSELAH
                     KRRLSSMGPGGLSRERAGFEVRDAHATHYGRICAVETPEGANIGLVLNMATYARVNQY
                     GFLETPYRKVINAVSASDAEGHIAAVDLEDGDGKTIVKKGAKITAADAKKLASVKNIV
                     TWPIKALVTDEIVYLDASGEGKAVIAGFGNEIDDQGYFVEERVSARNRLVAGEVDADS
                     VTHVDAASKQIIGSSAALVPFIEKNYVLRSLIGSNQQRQAVPLINPKSPIVGTGIESY
                     VARNTGQLVTAEADGTVLSATADEVVVDYGKVKKTYNPLHYIRSNEGSSINQKVAVSS
                     NDKVKKGDVLIEGMSIENGELALGQDVLIAFMPWGGYNFEDAIVISERLVKEDAFTSV
                     HIIDYMTEVRETKLGPEIVTRDIPNVSEEALRHLDDDGIVRIGAEVHPGDILVGKITP
                     KGEQELSSEERLLRAIFGEKAKDVRDTSQRMSNGKHGKVVGVKIFSREKGHELKAGVI
                     MQIQVFVAQMRKVAVGDKLAGRHGNKGVIARVLPVEDMPFSADGTPVDVVLNPLGVPT
                     RMNLGQLFETHLGMAAHLLGMKVASPPFDGVPLEKIESLLKEAGLPADGKQQLYDGRT
                     GEALKERTTVGMMYMIKLNHMIADKIHARSTGPYTMVTQQPLGGKAQNGGQRFGEMEV
                     WALEAYGAANTLQEMLTIKSDDVYGRAKAYESIIKKTEIVGPKVPESFNVLVKELQGL
                     GLKVDLVGSNQAEIDAEALLQESIKEESENQSDIDVPQQENNVLDVTEDAVADEFMVL
                     DMQEETVEATVAPDGDKDEEVTL"
     gene            2988..>6007
                     /gene="rpoC"
                     /locus_tag="PKV52_01130"
     CDS             2988..>6007
                     /gene="rpoC"
                     /locus_tag="PKV52_01130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPD98849.1"
                     /translation="MRNYSTGTNISDFDAVRLSVASPEEVLSWSYGEVLKPETINYRT
                     QKPERDGLFCERIFGPVKDINPHDAKYKGMRSREAAVDKNGEIVTKSIVRRERMGHIS
                     LASPVAHIWFLRGTPSAIGLVLNMTVKNLERIAYFASYVILDVKTEKRDQMLLDKEAE
                     AAAAKTAIEARFEQLAAEENADVKKLATERSKELEDTLAEYEKLKAQLESLVENALIS
                     ENDYRNLPDDVQELATVGMGGEAIKKMLTKVDLESLITTLQSDVEEAKGQRKKKIMKR
                     LRILENMQKAGIKPYSMCLSVIPVIPPDLRPMVQLTGGRFATSDLNDLYRRVINRNNR
                     LKKLVDLNAPEVIKRNEMRMLQEAVDALIDNNAARSGRAVSSTGQRRRLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVAGPELKLDECGLPKMMALELFKPFVIGNLIANDYA
                     HNIRSATRLIEAGETTVWDALDEAIKGKYVLLNRAPSLHRLSIQAFKPRLIEGKAIQL
                     HPLVCAGFNADFDGDQMAVHLPLSDKAQYEAREIMAANKNLLKPADGTPILHIEQDIV
                     LGTYYMTYDKYKDETPRSYSDINEAIMALDEDVIKLQTPITLLFRDERITTTVGRALF
                     NELLPTDFPYQNQAMTKKAIKKVMAQIYEKYGQERTAEIADELKDLAFEYATISGLSM
                     GMDDFPEIVGIDKAIAGGESRSAEISSQYEAGMLTNDERYRLTVEGWVKVDGQVQNML
                     AEQLKHQDNSMAIAVTSGARGNISQMKSAVGLLGVLSDTSGRAIELPVKSGYKHGLSQ
                     LEYFTGTRGVRKALIDIALNTADSGYLTRRLVDVSQDVFTVDEEADDPGFPIYRSDSD
                     ETGIPFAARLEGRYAAEDFVVDGATIVAKGELITRSAAQAVSDSTLDAVKIMSVLTSG
                     TIRGVSRKSYGLDPATGNIVENRHPVGVIAAQSIGEPGTQLSLDSKHRAGAVIADETA
                     QGLTRVEELFEVRTPKGQAYLADISGVVSVKEAG"
BASE COUNT         1885 a         1211 c         1444 g         1467 t
ORIGIN      
        1 gatcgtcgcc gtaaaatacc tgtgactact cttctgaagg cgcttggcat gagcgaaacg
       61 gctataaaag aagctgttaa gcacgtgaac actggcacaa ccgattacgt agctgcaact
      121 attgaaaaag atccaagtaa aggtgctcaa gaagcattaa ttgaagtata ccgacgcttg
      181 cgaccgggcg atcttgcaac aatagacaac gcgcgaagtc tcattgaaag catgttttac
      241 acctataagc gttttgactt tagccgagtt ggtcgttaca aaatcaatca acgattagac
      301 ctagacgtag aaaatacctt agaaaaccga gttatgcgac ttgatgacat gttggcaatc
      361 gtgtcagaaa ttatacggtt aaacaacacg caagaaccag ccgatgatat agatagcttg
      421 gcaaatcgac gcataaaact cgtaggcgag cttgtgcaac gccaatttag aattgggtta
      481 ttgcgcatgg atcgcaatgc gaaagatcgt atgagcatga gcgaaataga aacagtgagt
      541 ccaagccagc tggtaaatgc acgcccaatc gttgctgctg ttcgcgaatt ttttgcgagc
      601 tctcagttat ctcagtttat ggatcaaatc aacccacttt cagaacttgc tcacaagcgt
      661 cgtttaagct caatgggacc tggtggtctt tcacgagaac gtgcaggttt tgaagtgcgt
      721 gatgctcacg caacacacta tggccgcata tgcgcagtag aaacaccaga aggcgcgaat
      781 attggtcttg tacttaatat ggccacctat gcacgcgtta atcagtacgg ctttttagaa
      841 acaccgtacc gaaaagtcat taacgctgta tctgcgtctg acgcagaggg tcatattgct
      901 gcggttgatt tagaagacgg cgacgggaaa actattgtga aaaaaggtgc taaaatcacc
      961 gctgctgatg caaaaaaatt agcgagtgtt aaaaatatag tgacatggcc aatcaaggca
     1021 cttgtaacag acgaaatcgt ctatcttgat gcgagtggcg aaggcaaagc cgtcattgca
     1081 ggttttggta acgaaattga tgaccaaggc tacttcgtag aggaaagagt ttcagcacgt
     1141 aacaggcttg tcgcaggaga agtagatgcg gatagtgtaa cgcacgtaga tgccgcgagt
     1201 aagcaaatta ttgggtctag tgccgcacta gtgcctttta tagaaaagaa ctacgtactc
     1261 cgtagtctta ttggaagtaa ccaacaacga caagcggtac cactcattaa tccaaagtca
     1321 ccaattgtcg gtactggaat agaaagttat gtcgctcgta acactgggca gctagttact
     1381 gctgaggctg atggaacggt actcagcgca accgctgatg aagtagttgt agattatggc
     1441 aaggttaaaa agacctataa ccctctgcat tacattagaa gtaatgaggg ctcaagtatt
     1501 aaccaaaaag tagcagtatc tagcaacgat aaagttaaaa aaggtgatgt acttatagaa
     1561 ggtatgagta tcgaaaacgg cgaactagct ttaggccaag acgtcctgat cgcctttatg
     1621 ccgtggggtg gctataattt tgaagacgcc atcgttatta gcgaacgact tgtcaaagaa
     1681 gacgcattta cgagcgtaca catcattgac tatatgactg aagtacgcga aaccaaactc
     1741 ggaccagaaa tcgtcaccag agacattcct aacgtatcag aagaagcgct tcgtcactta
     1801 gatgacgacg gcatagtacg cattggtgca gaagtacacc caggtgatat tttagtagga
     1861 aaaattaccc ctaagggcga acaagagctt tctagcgaag agcgattatt gcgagcaatt
     1921 tttggtgaaa aagccaaaga tgtgcgtgat acctcgcaac gtatgagcaa tggtaagcat
     1981 ggtaaagttg tgggtgtaaa aatctttagc cgtgaaaaag gccacgaact aaaagccggt
     2041 gtaattatgc aaattcaagt gtttgttgca caaatgcgta aagttgcagt tggtgacaaa
     2101 ttagcgggtc ggcacggaaa taagggtgtt atcgctcgtg tcttacctgt agaagacatg
     2161 ccgttttctg ctgatggtac tccagtagac gtcgtgttaa acccccttgg tgtgcctact
     2221 cgtatgaacc ttgggcaatt gtttgaaaca cacctcggta tggctgctca tttacttggt
     2281 atgaaagtag cgagtccgcc gtttgatggc gtaccactag aaaagataga aagtttacta
     2341 aaagaagctg gtttgccggc tgatggtaaa cagcaactgt atgatggacg caccggcgaa
     2401 gcacttaaag aacgaacgac agttggcatg atgtatatga ttaagcttaa tcatatgatc
     2461 gccgacaaga tacatgcacg ctcaacgggt ccgtacacaa tggttaccca gcaaccactt
     2521 ggtggtaaag cccaaaatgg tggccagcga tttggagaaa tggaagtgtg ggcacttgag
     2581 gcgtatggtg cggctaacac cttgcaagaa atgctcacca ttaaatcaga tgatgtctat
     2641 ggccgtgcta aggcatatga gtctattatt aaaaagaccg aaattgttgg accaaaggtc
     2701 ccagaaagct ttaatgtgct tgttaaagag ctgcaaggac taggccttaa ggtagacctt
     2761 gtggggagca atcaggcaga aattgacgcc gaggcgttgt tgcaagaaag tataaaagaa
     2821 gagtcagaaa atcaatctga cattgatgtt ccacagcaag aaaataacgt cttagacgtg
     2881 accgaagacg ctgttgctga cgagtttatg gttctagata tgcaggaaga aacagttgaa
     2941 gcaacggttg ccccagatgg cgacaaagat gaggaggtaa cactataatg cgtaattact
     3001 ctaccggtac gaacatatcc gactttgatg cagtgcgact atctgttgcc agcccagaag
     3061 aagttctatc atggagctac ggagaagttt taaaaccaga aacaattaac tatcgtacac
     3121 aaaagccaga acgtgatggt ttattttgtg aaagaatttt tggccctgtc aaagatataa
     3181 acccacacga tgcaaaatat aaaggtatgc gttcacgtga agccgcagtt gataagaacg
     3241 gtgaaattgt cactaaatct atcgttaggc gcgagcgcat gggccacatt agtctcgcga
     3301 gtcccgtcgc tcatatttgg tttttacgtg gtacgccaag tgctattggt ttagtgctaa
     3361 atatgacggt taaaaaccta gaacgaattg cctactttgc aagctacgtt attctagacg
     3421 ttaaaacaga aaagcgtgac caaatgctgc tagacaaaga agcagaagcc gccgctgcta
     3481 aaacagccat cgaggctcgt tttgagcaac tcgctgcgga agaaaacgca gacgttaaaa
     3541 aactcgctac agaacgcagc aaagaactcg aagacacttt agcagagtac gaaaaactta
     3601 aagctcagct ggaaagttta gtagaaaatg cgcttataag tgaaaacgat taccgcaatc
     3661 tgccagacga tgtacaagaa ctagcaactg ttggtatggg tggcgaagca atcaaaaaaa
     3721 tgcttactaa ggtagattta gaatcgctta ttacaacact gcaaagtgat gtcgaagaag
     3781 cgaaaggtca gcgcaaaaag aaaattatga agcgcttacg tatcttagaa aacatgcaga
     3841 aagcggggat taagccctat agcatgtgct tatctgttat acctgtaatt cctccagatc
     3901 ttcgacctat ggtgcagctt actggtggtc ggtttgcaac gagcgatctc aatgatttgt
     3961 atcgccgtgt aatcaaccgt aacaaccggc ttaagaaact tgtagatctt aatgctccag
     4021 aagtaattaa gcgcaacgaa atgcgtatgt tacaagaagc tgtagatgcg ttaattgata
     4081 ataacgctgc ccgcagcggc cgagccgtaa gcagtactgg tcagcgacgt cgactgaaga
     4141 gcctcagtga tatgctcaaa ggtaagcaag gtcgttttcg ccaaaactta cttgggaaac
     4201 gagtagatta ttctggccgt tctgtaattg tagctggtcc tgagctcaaa ctagacgagt
     4261 gcggtctacc aaagatgatg gcgctagaac tgtttaagcc gttcgtcata ggaaatttaa
     4321 ttgcaaatga ctacgctcat aatattcgtt ctgcgacacg tctaattgaa gcaggtgaaa
     4381 cgacagtttg ggacgctctc gacgaggcca tcaagggtaa gtacgttctt cttaacagag
     4441 caccgagctt gcaccgtttg agtattcaag cctttaaacc acgcctaatt gaaggtaaag
     4501 ctattcagct acacccactt gtctgtgctg gttttaatgc tgattttgac ggagaccaaa
     4561 tggcggtaca tctgccactg tctgacaaag ctcagtatga ggctcgcgaa atcatggctg
     4621 ctaataaaaa cctattgaaa cctgccgatg gaacaccaat tctacatatc gaacaagata
     4681 tcgtacttgg cacatactac atgacgtacg ataaatacaa agatgaaaca ccacggagct
     4741 attcagacat caacgaagca atcatggcac tagacgaaga cgtcataaag ttacaaacgc
     4801 caatcacact actattccgc gatgaaagaa tcactaccac tgttgggcga gctttgttta
     4861 atgagttgtt gcctactgat tttccttacc aaaatcaagc gatgaccaaa aaagctatta
     4921 aaaaagtgat ggctcagata tacgaaaaat atggccaaga acgcactgca gaaattgccg
     4981 atgagctaaa agatctcgca tttgaatatg caacaatatc tggtctttct atgggtatgg
     5041 atgacttccc tgaaatcgtg ggaatagata aagcaattgc aggcggtgaa tcccgctcgg
     5101 ccgaaattag ttctcagtac gaagcaggta tgcttaccaa cgatgagcgc tatcgattaa
     5161 ctgttgaagg ttgggtaaaa gttgatggcc aagtacaaaa tatgcttgca gagcagctaa
     5221 agcatcaaga taattctatg gctattgctg tgacatcagg tgctcgtggt aatatcagcc
     5281 aaatgaaatc tgcagttgga ctgcttggtg ttctttcaga cacaagtggc cgagcaattg
     5341 agctcccagt aaaatctggc tataagcatg gtctatctca gctagaatac tttacgggta
     5401 ctcgtggtgt acgaaaagcc ctcattgaca ttgcacttaa tacagcagac tcagggtatc
     5461 tgacacgccg tttagtagat gtaagtcaag atgtctttac cgtagacgaa gaagccgatg
     5521 atccaggatt tccaatttat cgttcagatt cagacgaaac cggtatacct tttgcagcac
     5581 gcctagaggg tcgttatgca gcagaagatt ttgtggtaga tggcgcgacg attgtagcaa
     5641 aaggtgagct cattacacgt tctgctgctc aagcggtgag cgatagcaca ctagatgcag
     5701 ttaagataat gagcgttctt acgagcggta cgatacgagg agtctctaga aagagctatg
     5761 ggcttgatcc tgcgactggt aatattgtag aaaatcgtca cccagtaggt gtaatcgccg
     5821 ctcaaagtat aggtgagccg ggtacgcaat tgtcactaga ctccaaacac cgagcaggag
     5881 ctgttattgc cgacgaaacc gcacaaggtc taacacgtgt agaagagctg tttgaagtac
     5941 gtacaccaaa aggtcaggca tatttagcag acataagtgg tgtagtaagc gttaaagaag
     6001 caggtga
//
LOCUS       DAOPLS010000027         5856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_14655_length_5856_cov_3.800207, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000027 DAOPLS010000000
VERSION     DAOPLS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14655_length_5856_cov_3.800207"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..791
                     /gene="trmD"
                     /locus_tag="PKV52_01135"
     CDS             126..791
                     /gene="trmD"
                     /locus_tag="PKV52_01135"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPD98850.1"
                     /translation="MKIYIISLFPDMFAGVLGNSMLWKAQDKQLIDFEVVPLREYGLG
                     PRKMVDDTPYGGGDGMLLMIEPLFAAVNYCKKSSPSARVLLMTPRGYDYTQSDAQRLA
                     SAGADLIIICARYEGYDERITTIVDEQICIGHYVLTGGELAAMVVADSVTRLIPGVLG
                     GEKSAEIESFSEGNNREYPQYTRPEEYNGLHVPKVLLSGHHAEIDKWRQDQSNRFTKP
                     SIK"
     gene            840..1229
                     /gene="sodN"
                     /locus_tag="PKV52_01140"
     CDS             840..1229
                     /gene="sodN"
                     /locus_tag="PKV52_01140"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HPD98851.1"
                     /translation="MIKPVYAHCDIPCGIYETDSMRHAAETCLRMIEKIEALGELDSQ
                     DKWNNFVRMVDIKEKHAQRVKDEAYILWSDYFKPEHLTRFPQLHDVFWRLAKQASIVK
                     QTVDKAACEQLLHNVTEADTLFTDSKK"
     gene            1234..1536
                     /locus_tag="PKV52_01145"
     CDS             1234..1536
                     /locus_tag="PKV52_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPD98852.1"
                     /translation="MLLRRVVGNSMQPSLHSGDYIICKKARHLKIGDIVVAEVNKREV
                     IKRITNIEKEYITLQGDNAPYSTDSRVYGPVAQNSIKAIYLAKLRIPRFVKNRLSH"
     gene            complement(1505..2353)
                     /locus_tag="PKV52_01150"
     CDS             complement(1505..2353)
                     /locus_tag="PKV52_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPD98853.1"
                     /translation="MKFVKIILLATLLFIFVWGVYYIMARQGDGIKSAQQDNVEIQQE
                     PTPLPPSFDNEALQTKLQEVLDQYPTLETAVAVQSTTDNQSVAIDADAAYTAASTTKV
                     IVATYALKQIEQGAVSFTTYISGETLQAHLEKMIGKSDNDSWKSLLEYFGYKKIGAYA
                     NENGAPSFDAIANTIPPQDMANFLKNLQAGSLINQGNVQYLENLMKESYTGPIALSDE
                     HSTIIRKAGWLGDRAHLVGVITSGDKAVSYAIYTKTISNSAYPFTSGSALINELLSAI
                     TDSLQI"
     gene            2460..3695
                     /locus_tag="PKV52_01155"
     CDS             2460..3695
                     /locus_tag="PKV52_01155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HPD98854.1"
                     /translation="MNDKKHIVIIGAGTAGVMLANKFAKTSDVMVTVVDPAEFHYYQP
                     GFLLYPFNRHSFDEIHKRTSTLLHEHVYLLQQHVTDIAPSKKTIVTEDGTEISYDVLI
                     IATGTQIDPLLTDGLMNEQWHISIFDFYTPDGAEAVRGALQAFEGGRLVVQITEMPIK
                     CPVAPLEFVFLADDYFKKKGVREQVELLFVTPLSGAFTKPVASEKLGHLLTEKNIQVV
                     TDFYTEKVDGPNKTVYCYDGRSFDYDMLVTIPTNVGTKLLQKQDFTDDLGFVEVDPHT
                     LQSSKYPDIFAIGDSTNVATSKAGSVAHFEAHVVQENVLAYLYNQPLTGFFDGHSNCF
                     VETGGGKSLLLDFNYDTQPYEGTFPFAGIGPMKLLRPSRINHLGKLAFRYIYWYLLLP
                     GRPIPFIPNKMSLKGKKVV"
     gene            3708..4016
                     /locus_tag="PKV52_01160"
     CDS             3708..4016
                     /locus_tag="PKV52_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPD98855.1"
                     /translation="MQKEIAGQHIDVDIEGYMTNPDQWNGDIAQALASELHIELTQKH
                     WDVLNWLREQHYQGQEVNIRKVGKSGIVDIKQFYQLFPGGPLKNASKIAGLKKPTSCL
                     "
     gene            4027..4527
                     /locus_tag="PKV52_01165"
     CDS             4027..4527
                     /locus_tag="PKV52_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPD98856.1"
                     /translation="MEETVQQPQMGAQHLKKMMIVVSKSGIDGVYAGLIMANGARSEG
                     IEVDMFFTFFGLDAITKKRMEHLKVAIAGNTGMHMPELVGVFPGMENLATHMMMKEME
                     KLDVPPVPEFLEIIKAADGHIFGCKLAMEMFHLGKEDLWEGVDDVLSVGQFYNRYEPG
                     SQIIFI"
     gene            complement(4542..5387)
                     /locus_tag="PKV52_01170"
     CDS             complement(4542..5387)
                     /locus_tag="PKV52_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPD98857.1"
                     /translation="MSTLWQVFIFSLIGGIVSLIGGILLLANKNNAHRLALYATPFAA
                     GALLAAAFIDLLPEAAHQGNVDTALYATLGGILVFFMLERFLRWFHHHHEHEHAQNSS
                     KSDPRVPLIIVGDTVHNFIDGIAIAAGFLVDPATGIIVTLAVAAHEIPQEIGDFGLLL
                     SKGVARKNVIVINVLSALATTVAAVVFFTIGQSHTIPMDVVLGIVAGFFIYVAVSDII
                     PSIHKSEDKVIAGPQTLLLLIGVFIVGSITSVLHQYIDSGHDTHNESETHLLEESVDY
                     HQEDN"
     gene            5484..>5856
                     /locus_tag="PKV52_01175"
     CDS             5484..>5856
                     /locus_tag="PKV52_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPD98858.1"
                     /translation="MQTTLLKERLKAHGMSITKPRTRIFEYFLMAERPVTIQELVAEN
                     NTIHYATIYRTIHSLMQAGVIQQVPIGFTYTYELSDVFKPHHHHVTCEVCHKSMIVHS
                     RDIENLMHRVTSMEMFLMVWVI"
BASE COUNT         1861 a         1125 c         1241 g         1629 t
ORIGIN      
        1 gattgcttca aaaaagttaa gcaaaaggga gctttatgtg ataaaaacac ccggtataaa
       61 caccgggtgg ttttatttta tggcactaac aatagtaacc aaactgtttg ctatactatt
      121 aacagatgaa aatatatatc attagtttgt ttccagatat gtttgcaggt gtacttggca
      181 atagtatgct ctggaaagcc caagataagc agcttattga ctttgaagtt gtgccgctgc
      241 gcgaatatgg gcttggccca cgaaagatgg tagatgacac gccatatggt ggcggcgacg
      301 gtatgctttt aatgatagaa cctctgttcg cagcagtaaa ttattgtaaa aaaagttcgc
      361 cgtctgcaag agtgctcctc atgacgcctc gtggctatga ctacacacaa tcagacgcac
      421 aacgcttggc aagtgccggg gcagacttaa taattatttg tgctcgctac gaaggctacg
      481 atgaacggat tacgacaatt gtagatgaac agatctgtat aggtcactac gtgctaacag
      541 gtggagagtt agcggcaatg gttgtagccg atagtgtgac acggctgatt cctggtgtcc
      601 ttggtggtga aaaaagtgca gaaatagaaa gttttagcga aggtaacaac agagaatatc
      661 cgcagtacac acgcccagaa gaatataatg gcttacacgt acccaaggtg ctccttagtg
      721 gtcatcatgc agaaattgat aagtggcgcc aagaccaaag taaccgtttt acaaaaccat
      781 ccataaagta ggtatactaa tatatataaa tggaggtgat gcgtgtgata atacgaaaaa
      841 tgattaaacc agtgtatgca cattgtgata taccgtgtgg gatatacgaa acagacagta
      901 tgcgccacgc ggcagagact tgtctacgta tgatagaaaa aattgaagca cttggtgaac
      961 tagatagcca agataaatgg aataactttg ttcggatggt agatattaaa gaaaaacacg
     1021 cccaacgtgt gaaggatgaa gcatatattt tatggagcga ttactttaaa ccggaacatc
     1081 ttaccaggtt tccgcagcta catgatgtat tttggcgcct cgccaaacaa gcaagtatcg
     1141 ttaaacaaac ggtagataaa gctgcgtgtg aacaactatt acataacgtt acagaagcag
     1201 atacactatt tactgattca aaaaaataat tctatgttgc taaggcgcgt cgttgggaac
     1261 agtatgcaac caagtctgca ctcaggtgat tacataatat gcaagaaagc acgtcatcta
     1321 aaaataggcg acattgttgt cgcagaagtt aataaacgag aagtgataaa acgcatcact
     1381 aacattgaaa aagaatacat tacactccaa ggagataatg ctccctacag tacagacagc
     1441 cgtgtatatg ggcctgttgc acagaacagc ataaaagcta tctatctggc caagttacgt
     1501 attcctagat ttgtaaagaa tcggttatcg cactaagtaa ttcatttatg agcgccgatc
     1561 cagacgtaaa tgggtacgct gagttactta ttgtttttgt gtaaattgca tatgaaacag
     1621 ctttgtcacc agatgtaatg acacccacaa ggtgcgcacg atcgccgagc cacccagcct
     1681 ttctaattat tgtactgtgt tcatcactaa gtgctatcgg cccagtgtat gattctttca
     1741 ttaagttttc taaatactga acatttcctt gattaataag acttcctgct tgaagatttt
     1801 ttagaaaatt ggccatgtct tgcggtggga tagtatttgc aatcgcatca aaactaggtg
     1861 ctccgttttc attggcatat gccccgattt ttttatagcc aaaatattca aggagacttt
     1921 tccaagagtc attatcactt ttgcctatca tcttttctag gtgagcttgt aacgtttcac
     1981 cacttatgta ggtggtaaag ctgacagcgc cttgctcgat ttgcttaagt gcataggtag
     2041 ccacgattac ttttgttgta cttgctgctg tgtaagctgc atcagcatct atagcaaccg
     2101 attgattatc ggttgtagac tgaaccgcta ctgctgtctc tagcgtggga tactgatcta
     2161 aaacttcctg tagtttcgtt tgcaacgctt cgttatcaaa agaaggcggt agtggtgtgg
     2221 gctcttgttg aatttctaca ttgtcttgtt gggcgctttt aatcccatcc ccttgccggg
     2281 ccataatgta atatactccc caaacaaaga taaacaacag tgtagctaac agaatgattt
     2341 ttacaaattt catatacctc tgactattat accgtacaaa gttgaagaga acctgagtgg
     2401 ttagattgcc atattaagaa aacagcgtat tatattaagt ataaccatta tatataaaaa
     2461 tgaacgataa aaaacatata gtcataatag gtgctggaac agcgggtgtt atgctcgcta
     2521 ataagtttgc caaaacatct gatgttatgg tgacggtagt agatcctgca gaatttcatt
     2581 attatcagcc tggtttcttg ctgtatcctt ttaatcgcca ctcttttgat gaaatacaca
     2641 aaagaacaag tacgctgtta catgaacatg tttacttgct tcagcagcac gttacagata
     2701 ttgcaccaag caagaaaacg attgtaacag aagacggtac agaaatttct tatgacgttt
     2761 taattattgc tacgggtaca caaatagacc cactcctgac tgatggatta atgaatgaac
     2821 agtggcatat aagtatattt gatttttaca ctccagacgg agccgaagca gtccgaggtg
     2881 cattacaagc ctttgagggt gggcggcttg tagtgcagat aacagaaatg ccaataaaat
     2941 gccctgttgc tccactagaa ttcgtattct tagcagatga ttactttaaa aagaaaggcg
     3001 tcagggaaca agtagagtta ctatttgtca caccactctc tggagcattt actaagcctg
     3061 tcgcatccga aaagttgggc catttactta cagaaaaaaa catacaagtt gttaccgatt
     3121 tttacacaga aaaagtagac ggaccaaata aaacagtgta ttgctatgat ggtcgttcgt
     3181 ttgactatga catgttggtg acgatcccta cgaacgtagg tacgaagcta ctccagaaac
     3241 aagactttac cgatgattta gggtttgttg aggttgaccc acatacctta caatccagta
     3301 aatacccaga tatttttgcg ataggtgact ctacgaacgt cgccacttct aaagccggat
     3361 cagtcgcaca ctttgaggcg cacgttgtac aagaaaatgt gctcgcctat ctctataacc
     3421 agccattgac aggctttttt gacgggcact caaactgctt tgtagaaaca ggcggcggta
     3481 aatcgttatt gctagacttt aattacgata ctcagccata tgaaggtacc tttccatttg
     3541 ctggcattgg gcctatgaaa ctcttacgac cgagtagaat caatcatctt ggcaaattag
     3601 cattccggta tatatattgg tatttgctcc ttccgggtag gccaattcca tttataccaa
     3661 ataaaatgag cctaaaaggt aaaaaagtag tgtaaaaggg ggtatatatg caaaaagaaa
     3721 ttgcagggca acacatagac gtagatatag aaggctatat gaccaatcca gatcagtgga
     3781 atggtgatat cgctcaagcg ctggccagcg aactacatat tgaacttaca caaaaacact
     3841 gggacgtgct caattggcta cgcgaacaac actaccaagg acaagaagtt aatattcgta
     3901 aagtaggcaa gtctggcatt gtagatatta aacaatttta ccaattattt ccgggggggc
     3961 cacttaagaa tgcttccaaa attgccggat taaaaaaacc aacaagctgt ttataggaga
     4021 atacacatgg aagaaacagt gcaacaacca caaatgggcg cacagcacct aaaaaaaatg
     4081 atgatagtcg tttcaaaatc tggcattgat ggtgtatatg ccgggttaat tatggccaat
     4141 ggggctcgtt cagaaggcat agaagttgat atgttcttta cgttttttgg gcttgatgcc
     4201 attacgaaaa aacgaatgga acatttaaaa gtagcgatag ctgggaatac cggcatgcat
     4261 atgccggaat tagtaggtgt gtttccagga atggagaatc ttgcaacaca tatgatgatg
     4321 aaagaaatgg aaaaactaga tgttccgcca gtaccagagt tcttagaaat tattaaggca
     4381 gccgatggac atatttttgg ctgtaagctc gctatggaaa tgttccactt gggcaaagaa
     4441 gacctatggg aaggtgttga cgatgtacta tctgttgggc agttttataa ccgttacgaa
     4501 cccggttctc agataatatt tatctaagta tatcgtatgc tttaattgtc ttcttgatga
     4561 taatccacag actcttctaa aagatgcgtc tcagattcgt tatgggtatc gtgcccactg
     4621 tcgatgtatt ggtggagtac gctcgtaata ctaccaacaa taaacactcc tataaggagt
     4681 aatagcgttt gggggccagc aattactttg tcttcacttt tatgaatgct tggaataata
     4741 tcactgaccg caacgtaaat gaagaatccg gccacaatcc caagtacgac atccataggt
     4801 attgtgtgtg actgcccaat agtaaagaac acgacggcgg caactgtcgt cgctaatgca
     4861 cttaaaacat taataacaat gacattttta cgggccacgc ccttgctcaa cagcaagcca
     4921 aaatctccaa tttcttgtgg aatttcgtgc gcagcaacag caagcgtaac aataatacca
     4981 gttgctgggt ctactaaaaa gccggctgca atcgcgatgc catctataaa gttgtgtact
     5041 gtgtcaccaa caataataag cggtactctt gggtcagatt tgctagagtt ttgcgcgtgt
     5101 tcatgttcat gatgatgatg aaaccagcgt aagaatctct ctaacataaa aaagacgaga
     5161 atacccccaa gcgtcgcgta taaggcggtg tctacattgc cttggtgtgc ggcttctggt
     5221 agcagatcta tgaaagctgc cgctaaaagg gcacctgcag caaacggtgt tgcatacaaa
     5281 gctaaacgat gggcattatt tttgttagca aggagcaata ttcctccaat gaggcttaca
     5341 atgccgccaa tcaaactaaa aatgaatact tgccataacg tggacattta ttgacttctt
     5401 tctttacgat tttactattg tgctagttaa aatatacaag tctttatatt aaaattgcaa
     5461 attatttgca ataatatata aatatgcaaa cgacattact taaagagcgt ctaaaagcac
     5521 acggtatgag tatcacgaaa cccagaacac ggatatttga atactttctc atggctgaaa
     5581 gaccagtcac tatacaggaa ctcgtggcag aaaataatac cattcattat gccacaatat
     5641 acagaacaat ccattcgctg atgcaagctg gtgttataca gcaagtaccg attggtttta
     5701 catacacgta cgaattgagt gatgtattta aaccacatca ccaccacgtt acatgcgagg
     5761 tatgtcataa aagtatgatt gtacatagca gagacataga gaatttaatg cacagagtaa
     5821 ccagtatgga aatgtttttg atggtttggg taatcg
//
LOCUS       DAOPLS010000028         5796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_14891_length_5796_cov_4.260930, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000028 DAOPLS010000000
VERSION     DAOPLS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14891_length_5796_cov_4.260930"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /gene="topA"
                     /locus_tag="PKV52_01180"
     CDS             <1..2028
                     /gene="topA"
                     /locus_tag="PKV52_01180"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPD98859.1"
                     /translation="EGEAIAWHISEELKLDHTKTKRIVFHEITESAIKNALKHPRDID
                     IHLVDAQQARRVLDRLVGYELSPILWKKIRPGLSAGRVQSVAVRLIVEREREIENYTP
                     EITYKITGEFNKDSMVLVAELKNRLTSDKAVKKLLQTCAQSTFTVSAVTKKPGERNPS
                     APFTTSTLQQEAARKLGFSVKQTMTLAQRLYENGHITYMRTDSTNLSGLSLAATSQYI
                     TKTYGEAYAKTRTYKTKNSSAQEAHEAIRPTRVEVLQAGEDTNQTKLYQLIWKRTLAS
                     QMAPAKIERTDIDIAISSSTEVFSAKGEIIVFDGFYKVYGGNKEDQVMPNVKQGDQLH
                     YTEITAKQSYSRPPARYSEASLVRTLEELGIGRPSTYAPTISTIQSRGYVERADIEGK
                     QRTSEVYSLRAGEVQHTQESELAGADKNKLIPTHLAGVTTDFLVKQFGSIVNSGFTAQ
                     AETELDIIAQGEKKWQKAISDFYKQFHPLVIEAEGASRQEAAQAKLLGKDPKTGEPIY
                     ARYGKFGPMLQRGETQDDKKPAFAPMPENARIDTVTLEEALTMFTLPRLVGTTKDGKE
                     ITANIGRFGPYIQVDRIFVSIKPMGPFEVDEATAQRLYEEKLEQNAKKDIKTFAAEGL
                     NVLNGPFGAYVTDGSKNARIPKSIVPKDITLEQAKELIAKAKPPKKRKKSK"
     assembly_gap    2130..2229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2230..3101
                     /locus_tag="PKV52_01185"
     CDS             <2230..3101
                     /locus_tag="PKV52_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98860.1"
                     /translation="QLEKSVITRLKTAAEKDEIPGLAEVTTAIIDILQTNGHVMRVMQ
                     LSDLLTLQPSKISQARIAFFAELAPELSVIDESDHFYNAVGERSVHDEKHIREAVGKI
                     VDAIKKNGQPEPIEQIAASVHSSDTAHTQALASISKYLASLNGVWGLTKWPTVNPKNI
                     RDKIYVILSEKGSHMHFSEIADAIKESDFKRKDVTTQAIHNELIKDKRFVLIGRGIYA
                     LNEWGYKKGTVADIITEVLRKAGEPLHRDEIVKRVLKNRYVKETTILLNLQGKPQFKR
                     VAKATYALDEASL"
     gene            3206..>4272
                     /locus_tag="PKV52_01190"
     CDS             3206..>4272
                     /locus_tag="PKV52_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98861.1"
                     /translation="MADKASLLLVEIPRTNDKKELSAEQMFAALHGILKPKKSYFGDT
                     HQSQDKISFEIAAIGQRIRFYIWVPKHLQAFVEGQVYAQYPTAQISETEYDYTERPLN
                     QSVIHSTELVLTDNETLPIKTFPSFEVDPLAAITATLAKLNQPNEEMWVQVIVRPVSD
                     EWHKKSAKMVRRIKAGGWSSSGSGLSYLGQALGAIVKPPEPGASTTSYEPTERDKTRM
                     TAIEEKSRKLGYQVKVRIIYTGNDEATARQRMQAMVGTFKQFNTTNLNGFQSKYPSFD
                     RMKVNEYANRAFTDKGYILNIEEVASLYHLPHTSVETPNIVWATTKTAEPPSTLPTYG
                     SVPEESLSLFGITNFRGTHEM"
     assembly_gap    4273..4372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4373..5539
                     /locus_tag="PKV52_01195"
     CDS             <4373..5539
                     /locus_tag="PKV52_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HPD98862.1"
                     /translation="ILRYTILALLDYPNATLLDITRMLVDKPFRNDVIAHVDDPVVKN
                     FWLSEFASWNEKFASEAVAPVLNKVGAFTANPLVRNIVGQPKSTFNIRQAMDESKILI
                     VNLSRGLVGEDNAAILGSLFVTKIQLAAMSRADIPDLANRRPFYLYVDEFQNFATDSF
                     AVILSEARKYGLNLTVANQYISQMAEPVRDAVFGNVGSMVSFRVSPDDSPFLVKYFEP
                     QFEATDLSQFHNRHFVASMSINGEKASPFSATTLNIPPVLEDNSDKIIEHSRKTYAGD
                     LHTVEQQIRAVTLANTPKSSSPAQSNPLQQRNSSASTSTRPMSKHVIGVTATRAQQPE
                     PQKLSESQKRRLRRKRAAEAQARDAASATNPKPQAQSLDETNQTLTKDNTLHLR"
BASE COUNT         1893 a         1234 c         1162 g         1307 t
ORIGIN      
        1 gaaggtgaag ccattgcgtg gcatataagc gaagaattaa agctagacca tacaaaaacc
       61 aagcgaattg tatttcatga aattactgaa agcgccatta agaatgctct caaacaccca
      121 agagatatag atatacatct cgtagatgct cagcaagcac gccgcgtgct cgaccgcctt
      181 gttgggtatg aattatcgcc tattttatgg aaaaaaatta ggcccggatt aagcgccgga
      241 agggtgcaat ctgtcgctgt gagactcatt gtagaaagag aacgagaaat agaaaattat
      301 acgccagaaa ttacctacaa aataaccggt gaatttaaca aagattctat ggtgctcgtt
      361 gccgaactca aaaaccgcct tacgtcagat aaagcagtga aaaaattact acaaacctgt
      421 gctcaaagca cctttacagt cagtgcagta accaaaaaac caggcgagcg taacccatcg
      481 gcaccattca ccaccagtac cttgcagcaa gaagcagctc gtaaattagg cttcagtgta
      541 aaacaaacga tgacactcgc gcagagattg tatgaaaacg ggcacatcac ctatatgcgt
      601 acagacagca ctaatctatc agggctgtca ctcgctgcga ctagtcagta cattaccaaa
      661 acgtacggtg aagcttatgc caaaacccgc acctataaaa caaagaatag ttctgcccaa
      721 gaagcacacg aagcaattag accaacaagg gtagaagtcc tacaagctgg cgaagatact
      781 aatcaaacaa agctgtatca acttatctgg aagcgaacat tagcgagcca aatggcacca
      841 gccaagatag aacgcaccga tattgacatt gccattagct cctcgacaga agtattcagc
      901 gcaaagggtg aaatcattgt atttgacggg ttttacaaag tatacggtgg taacaaagag
      961 gatcaagtaa tgcccaacgt caaacaagga gatcagctac actatacaga aattactgcc
     1021 aaacagtcgt atagccgacc acctgcacgc tattcagagg cgagtttggt acgcacgcta
     1081 gaagaactcg gcattggacg accaagcacc tatgcgccaa caattagcac tatacaatca
     1141 agaggctatg tagaaagagc cgacatagaa ggcaaacagc gtacttcaga agtctatagc
     1201 ttacgagctg gcgaagttca acacacacaa gagtccgaac tcgctggggc agataaaaat
     1261 aagcttattc caacacacct cgcaggggtg acaacagatt tcttggtaaa gcagtttggt
     1321 tctattgtga atagcggctt tactgcgcag gctgaaaccg aactagacat cattgcacaa
     1381 ggtgaaaaaa agtggcaaaa agcaattagt gacttttata aacaatttca tccactagtc
     1441 atagaagcag aaggcgccag tagacaagaa gctgctcaag caaaactact aggcaaagac
     1501 ccgaaaacag gcgaacccat ctatgcgcgg tatgggaaat ttggaccaat gctacaacgt
     1561 ggcgaaacgc aagatgataa aaaaccagcc tttgcaccaa tgccagagaa cgcccgcatc
     1621 gacaccgtta cattagaaga agcgctcacc atgtttacgt tgccacgact tgtcggtaca
     1681 acgaaagacg gcaaagaaat aaccgctaat attggccgtt ttggaccata tattcaagtt
     1741 gatcgtattt ttgtatctat aaaaccaatg gggccttttg aagtagacga agccactgcc
     1801 cagcgcctat acgaagaaaa gctcgagcaa aacgctaaaa aagacatcaa aaccttcgca
     1861 gcagaaggct taaatgtact taacggccca tttggtgcct acgttacgga cggctcaaag
     1921 aatgcgcgta ttcctaaaag tatcgtcccc aaagacatta cgctcgaaca agctaaagag
     1981 ttaatcgcta aagccaagcc ccctaaaaaa cgcaaaaaat ctaaataagc aataccgttt
     2041 tgttggggtt ttttccaata tgtttactaa tgacttataa aatacaatgt atgctaaata
     2101 gtctattttg tgctacaata atgttagtan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnng acaattagaa aaatcagtta tcacacgcct taaaactgct gcagaaaagg
     2281 acgaaatacc tggtttagct gaagtcacta cagcaatcat tgacattttg cagacaaacg
     2341 gccacgtaat gcgtgttatg caactatcag atcttctgac tttgcagcca tctaaaatta
     2401 gtcaagctcg catagctttc tttgctgaac tcgcgcccga acttagtgtt atagatgaaa
     2461 gcgatcactt ttataatgct gttggtgaac gctcggtaca cgatgaaaaa catatccgtg
     2521 aagctgtagg aaaaattgtt gatgcaatca agaaaaatgg gcagccggaa cccatagagc
     2581 aaattgctgc ttcagtgcac agtagtgaca ccgcgcacac acaagcactc gccagtatat
     2641 ctaagtactt ggcgtcgtta aatggtgtat ggggtcttac gaaatggcca actgttaacc
     2701 caaagaatat tcgcgataaa atatatgtta ttttaagcga aaagggaagc catatgcatt
     2761 ttagtgagat agccgatgct attaaagagt ctgattttaa gcgaaaagat gttaccaccc
     2821 aggcaatcca caatgagctc attaaagata agcgctttgt gctgattggg cggggcatat
     2881 atgcactaaa cgaatggggc tacaaaaaag gcacagttgc tgacattatt acagaggttc
     2941 ttcgcaaagc tggcgagccg ctacatcgtg acgaaatcgt taaacgggtg cttaaaaacc
     3001 gctacgttaa agaaacgacg attttgctta atctccaagg caaaccgcaa tttaaacgcg
     3061 ttgcgaaagc cacttatgca ctcgacgaag cttcattgta aaaacgttat ctaaaaaatg
     3121 catccctaac aagtgacttc atcattctat ttagcgcctt taaagggtat aatataagct
     3181 gttcttaaaa aatacttaag gttatatggc agacaaagcc tcactattat tagttgaaat
     3241 accacgcacc aacgacaaga aagagctctc tgcggagcag atgtttgctg cgttacacgg
     3301 aatattgaaa cctaagaagt catatttcgg tgatacccat cagtcgcaag ataaaataag
     3361 ctttgaaatt gctgcaattg gccaacgcat ccggttttat atttgggtgc caaagcattt
     3421 acaagccttt gtagaagggc aagtctatgc tcaatacccc actgcacaaa tatcagaaac
     3481 tgagtatgat tacacagaac gtccactgaa ccaaagtgta atacattcta ccgaactagt
     3541 attaaccgat aacgaaacgt tacctataaa aacctttcct agttttgaag tagacccatt
     3601 ggcagcaatc actgcgaccc ttgcaaagct taaccaaccg aacgaagaaa tgtgggtgca
     3661 ggtaattgtc cgccccgtga gtgatgaatg gcataaaaaa agtgccaaaa tggttcggcg
     3721 tattaaggct ggtggatggt ctagtagtgg cagcggtctg tcatatctgg ggcaagcgct
     3781 cggtgcgatt gtaaagccac cagagccagg tgcgtcaact acaagctacg agcccacaga
     3841 gcgcgataaa acacgcatga ccgcaataga agaaaagagc cgcaagctag gctaccaagt
     3901 aaaggttcgc attatctata caggtaatga cgaagctacc gcccgtcaac gtatgcaagc
     3961 catggtaggc acttttaaac agtttaatac cacaaatctt aatggttttc agagtaaata
     4021 ccccagtttt gaccgcatga aggtcaatga atatgctaac cgagcattta ccgataaagg
     4081 ctatattctc aatatagaag aggtagcgag tttataccat ttaccacata catcagtaga
     4141 aacaccaaat attgtctggg ctaccactaa aacagctgag ccaccaagta cattgcctac
     4201 ctacggctct gtaccagaag agagtctcag cctgtttggt attactaatt ttcggggaac
     4261 acatgaaatg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcttgcg
     4381 ttacacaatt ttagcgctct tagactatcc gaacgcaaca ttactagata ttacccggat
     4441 gcttgttgat aagccgttta gaaacgatgt catagctcat gtagacgacc ctgtcgttaa
     4501 aaacttttgg cttagtgagt ttgcaagctg gaacgaaaag tttgcctcag aagcagtcgc
     4561 gccggtactg aataaggttg gtgcatttac ggcaaaccca ctcgtacgaa atattgttgg
     4621 gcaaccaaaa agtaccttca atatccgcca agcaatggat gaaagtaaaa tattaatcgt
     4681 caatctatcc cggggacttg ttggtgaaga taacgctgcc attttagggt cgctatttgt
     4741 aactaaaata caattagccg cgatgagccg tgcagatata ccagatcttg ctaatcggcg
     4801 cccattttac ctgtacgtag atgaatttca gaactttgcc accgatagct ttgcggtcat
     4861 tttaagtgaa gccagaaaat atgggctaaa tttaacggtt gctaatcagt atatttcaca
     4921 aatggcagaa cctgtccgtg atgcagtgtt tggcaacgta ggcagcatgg taagttttcg
     4981 ggttagccct gatgatagcc cttttttggt caaatatttc gaaccacaat tcgaagccac
     5041 tgatctatca caattccata ataggcattt tgtcgccagc atgagtataa acggagaaaa
     5101 agcgagtccc tttagcgcca caacacttaa tatcccgcca gtgctagaag ataattcaga
     5161 taaaatcatc gaacattcca gaaagaccta tgcgggcgat cttcacaccg ttgaacaaca
     5221 aatacgagca gttacactcg cgaatacacc taaatcgtcg tcaccggcgc aatcaaaccc
     5281 tctccagcaa cgtaacagca gcgcatcaac gtctacgcgc cccatgagta agcatgtcat
     5341 aggtgtaacc gctacacgtg cccagcaacc agagccacaa aagttgagtg aaagccaaaa
     5401 aaggcgatta cgaagaaaaa gagccgccga agcgcaagcg cgcgacgctg cgtctgcaac
     5461 aaaccctaag ccacaggcac aatcgttaga cgaaaccaac caaactctta caaaagataa
     5521 tacgctccat ttgcgataat aggttcgccc actgtgaatt atgtggcaat atataaatta
     5581 tgaacatttt gcgaactttg ttaataaagc aacaataatt atatataaat agtaactcgt
     5641 gtatgtaaaa gaattatagc caaaccatag cgcgaaggtc acacaacgaa aatatataca
     5701 tatgggcaat gaaaaagtca cgaagcatat gaacatatac tttgtaatgc acaaaaagca
     5761 gtagcagagt agtcccccag actgctatgc ataatg
//
LOCUS       DAOPLS010000029         5717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_15171_length_5717_cov_3.093783, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000029 DAOPLS010000000
VERSION     DAOPLS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15171_length_5717_cov_3.093783"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..307)
                     /locus_tag="PKV52_01200"
     CDS             complement(89..307)
                     /locus_tag="PKV52_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98863.1"
                     /translation="MAKKKLEGKAKKQIDAALRRRHQQPALAAKHLREVLGYMSPDIV
                     WQQVARTNPSAVNELKAVLRTHHIGRPA"
     gene            complement(557..1288)
                     /locus_tag="PKV52_01205"
     CDS             complement(557..1288)
                     /locus_tag="PKV52_01205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD98864.1"
                     /translation="MELGKDSWMDMANRLRNGGPSAVLARFALMLAGNAALADLLEHA
                     ANSDKDSLTHIRNRGALEEDIKELLLSPESGALIVIDLVNFGQVNNQRGQSGGDKLLQ
                     IIAEGLTRVTSRRRNDIIYTQHSRTEPSQQDEEGYRVGGDEFVALLKNGDSLKNADIV
                     AITHTKLRQILEDPAVTEFLTSENDDPDKVISLFGIRAGACIIDANKHASYADVQSDA
                     DPKLGTVCEYQLRLVDGTYELDCTI"
     misc_feature    1351..1474
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1534..2064
                     /gene="rplJ"
                     /locus_tag="PKV52_01210"
     CDS             1534..2064
                     /gene="rplJ"
                     /locus_tag="PKV52_01210"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPD98865.1"
                     /translation="MALSKEQKHQVVADVAELLATSKLTVVAKYKGLSVKDMQALRKS
                     ATQSQTVIKVVKNRLVRQALMQTDALKDVDTGLLVEQLVYAFNSEDEVAPAQSLHVFA
                     KTNPSLEFVGAITPEGLFLHADDVKALANLPSKDQLRGQLVGTIAAPLTSFIRVLQGS
                     QRGILYALTARAESIN"
     gene            2084..2494
                     /gene="rplL"
                     /locus_tag="PKV52_01215"
     CDS             2084..2494
                     /gene="rplL"
                     /locus_tag="PKV52_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPD98866.1"
                     /translation="MSETDNKVTVPKEFAGIVKELDKLSALEISKLVTFLEEYWNVSA
                     AAPAVAVAAAPAADGGAPAADEKSEFDVVLKDAGSQKVAVIKAVKEITGLGLGESKAI
                     VDGAPKPVLEKANKEDAEAAKKALEEAGGTVELA"
     gene            2733..3965
                     /locus_tag="PKV52_01220"
     CDS             2733..3965
                     /locus_tag="PKV52_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPD98867.1"
                     /translation="MRTATKEYDHSLFRSGVLDLTSVKHTMNDVSAEYVPYVTPVYDR
                     LPLTARMDLLLPEDHMGNLTPLTDRQEYVGNNRLMQLDLATYSPIGSFKFRGAWNAIA
                     SVDYHQLLEHGVVTASVGNHAQGVAMGVRRLNELFDAHSFSGRNGRRVHADIYVPSSV
                     APVKLEGIKNIGGDDVSIVVHGNTYDEAYEAACQRTDNLKGATLIHPYDNDLTVSGQS
                     MVGYAVARELQKQQINPSEVILVVPVGGGGLLAGIMKALRTIYPDANARPEVVLAQLE
                     GADSAVQSWQYFKKHNQLKAVPVAGDVNAFADGAAVRTIGAHGLQALPYVSNTVLVTN
                     SQLANGYKRHLDSIYMNLMEVYANHSVDPFFGLQEPASMIADVAAYQYAQRQHGKTVI
                     HLKTGINVNQELVEQLLG"
     gene            4139..4648
                     /locus_tag="PKV52_01225"
     CDS             4139..4648
                     /locus_tag="PKV52_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98868.1"
                     /translation="MSDLPEKQVKRLKALIREAETNLQAAHELLVSLVGEDPEMTAHA
                     KENIGGKILEGVFDGQNMVGSDGKTYPVPANYASKSKLVQGDILKLTIGDDGAFMYKQ
                     IGPIPRKQVVGMLIQKEGHYFVEVGEKMYRVLLASVTYFKAKPGDQISVNIPEDDADA
                     EWAAIEAAL"
     gene            4739..5326
                     /locus_tag="PKV52_01230"
     CDS             4739..5326
                     /locus_tag="PKV52_01230"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPD98869.1"
                     /translation="MAKLYFRYSAMNSGKSTSLLQTAYNYEERGMSVIVTKPIVDTKG
                     DSMIVSRISTAREVDFLIPPDMDIAHHVRNLSLGSVACVLVDEAQFLQPQQVDQLFDV
                     AITDNIPTIAYGLRTDFQTHGFPGATRLLEIAHDLQELKTICRCGQKAIFNGRKIGGK
                     LVFEGSQVAIDGQDNVEYESLCGACYKSAQANDIL"
     gene            5385..>5717
                     /locus_tag="PKV52_01235"
     CDS             5385..>5717
                     /locus_tag="PKV52_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD98870.1"
                     /translation="MSQALYRKYRSRSFDEIVGQKGVVTALSHSLKRKQMSHAYLFTG
                     PRGVGKTSIARIFAYAINDVPYNDEELPVDIIEIDAASNRGIEQVRDLREKVRIAPIV
                     GPYKVYIID"
BASE COUNT         1756 a         1102 c         1289 g         1570 t
ORIGIN      
        1 caataggatt actatggttg tacatttcgc cctgggtact gcatggcaaa agcgtgcggt
       61 tgatggtgca ctacttttat gtggtgactc aggcgggacg gccgatgtgg tgcgtccgta
      121 ataccgcctt gagttcattg actgcacttg ggttggtcct tgcgacttgc tgccagacaa
      181 tgtctggtga catatatccg agtacttcac gcaagtgttt agcagcaagt gcaggctgtt
      241 ggtgacgtcg tcgtaaggca gcatcgattt gtttctttgc cttaccttcg agtttctttt
      301 tagccatgaa ctgatcaccc ctatttgttt gagagcctct gcctacagac ggttgtctgt
      361 aaacagagcc agtaaaacaa aaaaccgggc ttcttgcccg gtgccgtatt cttgtatttg
      421 ctcgttttta tgcaacaaaa gaatcagcac cataccgtag tagcactaga cctaaaatct
      481 gtgtatacat actgcgcgtt gctgttatta cttgttgcat atcgtatagt atgccttggc
      541 tagtttattt ttgcaactat attgtacaat ctagctcgta tgtaccatct acgagtcgga
      601 gctgatattc acatacggta cctaatttag ggtctgcgtc actttgtaca tcagcgtaag
      661 acgcgtgttt attcgcatca attatgcacg cgcctgctcg tatgccaaat aaggatatta
      721 ctttatctgg atcgtcattt tcactagtaa ggaattcggt gactgcgggg tcttctagta
      781 tttggcgaag tttcgtatgg gtgatagcaa caatatcggc attttttaat gaatcaccat
      841 tttttaatag tgcaacaaat tcgtccccac ccacacggta accttcttcg tcttgttggc
      901 tcggttcagt acgtgagtgt tgtgtgtata ttatatcgtt gcggcgccta cttgtgactc
      961 tcgtgagccc ctcggctata atttgcaata acttatcacc acctgattgg ccacgttggt
     1021 tatttacctg cccaaagtta acgaggtcta ttacaatgag cgctccactt tctggagaca
     1081 aaagtaactc ttttatatct tcttcaagcg ctccacgatt acgtatatgt gtcaagctat
     1141 ctttatctga atttgctgca tgttctaaaa gatccgccaa agcagcattt cccgccagca
     1201 taagagcgaa tctcgctaat actgcgctag gaccgccatt acgtagacga ttcgccatat
     1261 ccatccagct gtccttacct aattccataa taatattata tcataaaaca gtaataataa
     1321 caatatttgc ttgtcacgag gcatgatatc tgttacaatt acctttaacc aaagacagtg
     1381 acgtctctat aaagagatat aagaatgtca ccgaggtaaa gatttgtttt attgtctttg
     1441 gtgcacgtgt ggccaaagat tttttatttt taccacatat gcacagtaat aaattttggt
     1501 cgatataatt acataaccca cataaggata tacatggctt taagtaaaga acaaaagcat
     1561 caagtggttg cagacgtggc tgaactactc gctacgtcta aactaacagt agtcgctaag
     1621 tacaaaggcc tgtcagtgaa agatatgcag gcactcagaa aaagtgcaac acagtcacag
     1681 acggtcatta aggtagtaaa aaatagatta gtccgccaag cactgatgca aactgacgca
     1741 ctaaaggatg ttgatactgg tttgctcgta gagcagttag tgtatgcatt taatagtgaa
     1801 gacgaagtag cgcccgccca gagcctccat gtttttgcaa aaacgaaccc aagtttagag
     1861 tttgtaggag caattacacc agaaggttta tttttacacg cagatgacgt aaaagcactc
     1921 gcgaacttac caagcaaaga tcaattacgt ggtcaattag ttggtacaat tgcagctccg
     1981 cttacatcgt ttatacgtgt attacaaggg agccaacggg gtattttata tgccttaaca
     2041 gcccgagcag aatcaataaa ttaaggaaca aggagataat acgatgtcag agacagataa
     2101 caaagtaaca gtgccaaaag agttcgcagg cattgtcaaa gaattagata aattaagtgc
     2161 cctagaaatt agcaaattag taacgtttct agaagaatac tggaacgtaa gtgcagctgc
     2221 accagccgtt gcagttgctg cagcgcctgc agctgacggt ggtgcaccag cagctgatga
     2281 aaagagcgaa tttgatgttg ttcttaaaga cgctggtagc cagaaagtcg ctgttatcaa
     2341 agcagtcaaa gaaattaccg ggcttggcct tggtgaatca aaagctattg tagatggtgc
     2401 accaaaacca gtgctagaaa aagctaacaa agaagatgca gaagccgcta aaaaagctct
     2461 agaagaagct ggcggaactg tcgaattagc ataagtagct acctaagcac cttacggtgt
     2521 actaagcgct ccgttacgta cggagcgttt tttagttgac aacatgctgc tataaggcgt
     2581 ataaccgtaa gtatatgcaa gctatacgcg caattattgc catcattatt atgccgcacc
     2641 gagcggtctg tttaatgatg caataatagt gcaacaataa aaagacatcc gctcggcaac
     2701 taccgagcgg attttttata taaggagtaa ctatgagaac agcaaccaaa gaatatgacc
     2761 acagcttatt tagaagtggg gtcttagacc tcacgtctgt taaacatact atgaatgatg
     2821 taagcgcaga atatgtacct tatgtgactc cagtgtatga taggttacca ctgactgctc
     2881 ggatggactt attgttgcct gaagatcata tgggtaatct tacaccactc acagatcgcc
     2941 aagagtatgt tggcaacaac cggctcatgc aactagactt agctacatat tcacccatag
     3001 gttcatttaa atttcgtggc gcatggaatg ctattgcatc ggtagattat catcagctcc
     3061 ttgagcatgg ggtagttacg gcgagcgttg ggaaccatgc acagggcgta gcaatgggag
     3121 ttcggcgcct gaatgaatta ttcgatgcac attcttttag cggtagaaat ggacggcgtg
     3181 tacacgctga catatatgtt ccatctagtg tcgcaccagt aaaattagag ggtattaaaa
     3241 atattggtgg tgatgatgta agtattgtcg ttcatggcaa cacttacgac gaagcctatg
     3301 aagcggcttg ccaacgtaca gataatttaa aaggtgcaac acttattcat ccgtacgata
     3361 acgacctaac tgtaagtggg caaagtatgg ttgggtatgc tgtcgctcgt gagctgcaaa
     3421 aacagcagat aaacccaagc gaagtaatat tggtagtacc tgttggagga ggtggcttgc
     3481 tggcaggtat tatgaaagca ctccgtacaa tttacccaga tgctaacgca agaccagaag
     3541 ttgtattggc acagctagaa ggcgctgata gtgccgtgca aagttggcaa tactttaaaa
     3601 agcacaatca actaaaagca gtgccggtcg caggagatgt gaatgcattc gcagatgggg
     3661 ctgcggtgcg aacaatagga gcccacggct tacaggccct accatacgtt tctaatacag
     3721 tactagtgac taatagccaa ttagcgaacg gctataaaag acatctagat agtatctata
     3781 tgaatttaat ggaagtgtac gccaaccata gtgtagaccc attctttggg ttgcaagagc
     3841 cagcaagcat gatcgctgat gtagctgcat atcaatatgc tcagcggcaa catggtaaaa
     3901 ctgttattca tttaaaaact ggtatcaacg taaatcaaga attggtagag caactgctgg
     3961 ggtagtgttt tgttacttta tatacaacgc tattaatagc tgtatataaa tatataaaaa
     4021 tgttagtttt ccacagaata ggtacataat aattgcgtaa tatcgtatta tatgctacgg
     4081 ttatgacaag gactttaaaa ggtgttttag tagaaaaaca gaaaaaggag aagctgcgat
     4141 gtccgattta ccagagaagc aagtaaagcg cttgaaagcg ctaatcagag aagcagaaac
     4201 aaatttacaa gctgcccatg aactattggt gagtttagtg ggtgaagacc cagaaatgac
     4261 tgctcatgcc aaggaaaata taggtggcaa aatactagaa ggtgtgtttg atggtcagaa
     4321 catggttggt tctgatggca agacctaccc tgtgccagcc aattatgcta gtaagtctaa
     4381 actcgtacaa ggtgatatct taaagcttac cattggtgat gatggtgcgt ttatgtacaa
     4441 gcagattggg cccattccac gtaaacaagt agtaggtatg cttatacaaa aagaaggcca
     4501 ctactttgta gaagtaggcg aaaaaatgta tcgtgttcta cttgccagtg tgacttactt
     4561 taaggcgaaa cctggtgatc agataagtgt taatattcca gaagacgacg cagatgctga
     4621 atgggctgct atagaagctg ccttgtaaac tattagccaa cgtataatta agacgacatt
     4681 tagtcgtctt ttttatatac tagaacacag taatagtgca atcaataagg agtagcaatt
     4741 ggcaaagctc tattttcggt atagtgcaat gaatagtggt aagtctacat cgctattgca
     4801 aactgcatat aactatgaag aacgtggtat gtctgttatt gtcacaaaac ctatcgttga
     4861 tacaaaaggt gatagtatga ttgtgtctag aataagtacg gctagagaag tagactttct
     4921 aataccgcct gatatggata tcgcacacca tgtacgtaat cttagccttg gttcagtggc
     4981 ctgtgtcctg gtagacgaag cacaattttt acaaccacag caagtagacc aactgtttga
     5041 tgtcgcaatc acagataata tcccgacgat tgcttatggt ttacgaacag attttcagac
     5101 acatgggttt cctggagcga cccgcttatt agaaattgcc catgatttac aagaactaaa
     5161 aacgatatgc cggtgtggtc aaaaagcaat atttaatggg agaaaaatag gtggaaaatt
     5221 agtatttgaa ggctctcagg tggctataga tgggcaagat aatgtagaat acgaatcatt
     5281 atgtggtgca tgctacaaat cggctcaagc caacgatatt ctgtagtaac tacaacagac
     5341 aaataagtgc cttagcggta tactataggt aaaagagggg aagaatgtcg caagcgttat
     5401 atagaaagta ccgttcaagg tcatttgatg aaatcgtcgg ccaaaaaggc gttgtgactg
     5461 cattgtcgca ctctttaaaa cgtaaacaaa tgagtcacgc ctacttattt acagggccta
     5521 gaggcgtcgg aaaaacgagt attgcgagga tattcgccta cgctatcaat gacgtacctt
     5581 ataatgacga agagctaccg gtagatatta tagaaattga tgccgcgagt aatagaggaa
     5641 tagagcaggt tcgcgatcta cgtgaaaaag taagaatagc gccaatcgtc gggccatata
     5701 aggtgtatat tattgat
//
LOCUS       DAOPLS010000030         5509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_15967_length_5509_cov_4.149248, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000030 DAOPLS010000000
VERSION     DAOPLS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15967_length_5509_cov_4.149248"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="sbcB"
                     /locus_tag="PKV52_01240"
     CDS             complement(<1..776)
                     /gene="sbcB"
                     /locus_tag="PKV52_01240"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HPD98871.1"
                     /translation="MANDTLYFYDLETSGINPRSARIMQFAGQRTTLDLEPIGPPDNT
                     LIKMSDDILPEPQAILVTGIAPQQTIADGISEREFCRYFIESIATPYTTILGFNSIRF
                     DNEFIRFLLYRNFYDAYEWSYANGRSVWDMLDVVRMTRALRPDGIEWPFASDGRPSNS
                     LELLTSVNHLQHENAHDALSDVYATIAVAKLIKQTQPKLFSYLYAMRTKQAVQAVVNS
                     GEPFVYTTGRYNGAYEKTSVAVLLADHPVYPGNRALVYDLR"
     gene            complement(813..1820)
                     /locus_tag="PKV52_01245"
     CDS             complement(813..1820)
                     /locus_tag="PKV52_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPD98872.1"
                     /translation="MKHKVRSYSHQAYSMLFMSGVLWGFSALFYKKSLAVVSISVLLA
                     VRFILGAVFIYATERKKFVRLSVRVLGLIALFTIVDTVIINFLYSAGIQRTTAIHAAV
                     IHLSTPFIVYFLAAIFLSEQPHKRVIVGAVIAVMGMLGIILGSVSATNDTVSSVTGDI
                     LLFFMAFLTASAIVIGRKLLTKYKKIPGEQLGFIEYSLAVIPFGVFAIFSGNLSELGA
                     LNVSSWGWIIGASILCGAIPLTLYNRSIRTLPAPRLADVSFISPTTGALVGVFLLGEQ
                     ISASFVIGAALVIAGLLISHNKIHPVYIAHHLRADVSTLQAIFRLPKRAYEHIRVEVK
                     W"
     gene            complement(1810..3186)
                     /locus_tag="PKV52_01250"
     CDS             complement(1810..3186)
                     /locus_tag="PKV52_01250"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPD98873.1"
                     /translation="MEKSNISELALRTKIINTLPNANGQSVIYVMSRDQRITDNFGLA
                     YAQDYALRHKLPLAVVFCFSPVKGRAREHVVFMIEGLREIETTLAKLAIPFIALFGPH
                     DVVLRNIAHHTKPALMVTDYSPLRGTLRWQNVLSQTVPLCIVDSHNIVPPWLVSNKQE
                     YSARTIRPKIYSHYNDFIHHHSTIKKHPYRWPGAVMNLKEVAENLTLYINQHTPNNTH
                     SGFTAGETAAQQHLQHFIDTKLHGYAHSRNDPTKQHTSDLSPYLHFGQIGSVSVMNAV
                     SAAVVANAALQNDADALQEELVIRKELADNYCNYNQDYTTLRGAPEWSRRTLQKHETD
                     PRNYIYSLQELETANTHDLAWNAAQRQLTATGKMHGYMRMYWAKKVLEWSKDAQEAQQ
                     KLIYLNDFYSIDGGDPNGYVGALWSIAGLHDRPWGERPVYGTVRSMVYNGLKRKFDIE
                     AYIQKYET"
     gene            complement(3167..4450)
                     /gene="obgE"
                     /locus_tag="PKV52_01255"
     CDS             complement(3167..4450)
                     /gene="obgE"
                     /locus_tag="PKV52_01255"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPD98874.1"
                     /translation="MFVDIVTIDVLAGDGGNGIVSFRHEKFVDRGGPDGGDGGKGGDV
                     ILQATRNENTLATFRYQKRIQAEHGQAGSKRKKRGRSAKDTVVLVPIGTTIVNAETGD
                     VIADLTEDNQAAVIAKGGSGGFGNAHFTSSVRQAPRVAEKGEQGEQRTVILEMKLVAD
                     IGLIGLPNAGKSTLLSVVSNAKPEIANYPFTTTVPNLGVVDIDSHSLLIADIPGLIEG
                     ASKGKGLGDDFLRHVSRCKVLLHLIDVTSNDVATDYTMIQKELSAYNQELAQKQQIIV
                     FTKAELVDEEIIAMQKELLSEVIPDGSTICVVSAHAHTKIKELLHKAAKLVIEADEKQ
                     SELHADEDALPLITLEQNDTQWVASKVKDGLFLVTGKKIELFAKRTDYENDFGVMRLR
                     DILKKMGITNQLLKLGIEYGDHIQIGDPSCGKIQY"
     gene            complement(4546..>5509)
                     /locus_tag="PKV52_01260"
     CDS             complement(4546..>5509)
                     /locus_tag="PKV52_01260"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPD98875.1"
                     /translation="ASQGSSATLSFIGQQSSTDESAPLDTLSSADIAANVALATSMPE
                     EVMVINQADSYKAQLTQANTEESVVAKPQVVTGASESKNDITTYVAVEGDTVTKIAAQ
                     FGVSADSIKWSNGIAIEDIPSGKELKIPPSDGIVYQVADGDTIESLAAKYRASAEKLV
                     AVNDTELTGLKPGDTIFIAGGQPPVTPRASRTSSTAAANRSSNTAPAYNFVARYGGNG
                     YYYGYCTWYAANRVAVPSNWGNANTWDNRARSSGWTVSKTPVPGAVAQTDAMSWWGHV
                     AVVEAVSADGTMMKYSDMNGIAGFGRVGYSDWVPTSTFQNYIYR"
BASE COUNT         1526 a         1213 c         1091 g         1679 t
ORIGIN      
        1 cgtaaatcat acacaagtgc acgatttccc ggatagactg gatgatctgc tagtagtaca
       61 gcaacagacg ttttttcgta tgcaccgtta taacgaccgg tcgtataaac aaaaggctct
      121 ccgctattaa ctactgcctg tactgcttgc tttgttcgca tggcatacaa gtacgaaaat
      181 agttttggtt gtgtttgctt tataagtttt gccacggcaa tggtagcata cacatcagat
      241 agtgcatcat gtgcgttttc gtgctgcaaa tgattaacac ttgtaagcag ctccagcgag
      301 ttagaaggcc ggccatcgct tgcaaagggc cactctatac catctggtcg caaggctcgt
      361 gtcatcctaa cgacatctag catatcccat acagatctac cgttagcgta tgaccactcg
      421 taagcgtcat aaaaatttct gtaaagtaag aatcgaataa attcgttgtc aaatcgaata
      481 gaattaaaac cgagtatagt agtatagggt gttgcaattg attcaataaa atagcgacaa
      541 aattctcgct ccgaaattcc gtcggcaatt gtttgctggg gtgcgatacc tgtcactagt
      601 atcgcctgcg gttcaggcaa aatgtcatct gacattttaa tgagggtgtt atctggtggt
      661 ccaattggct caagatccaa tgttgtccgc tggccggcaa attgcataat acgtgcactg
      721 cgcggattaa tgccacttgt ttctaaatca taaaaatata atgtgtcatt cgccataatc
      781 taaaccaatg gtagcagaaa tatatacaaa tactaccatt taacttctac acgaatgtgc
      841 tcatatgcac gcttcggtaa cctaaatata gcttgcagag tacttacatc agcacgcaaa
      901 tgatgggcta tataaactgg atgaatttta ttatggctta taagtagtcc agctataact
      961 aatgcagcgc caatcacaaa agacgctgat atttgttcac ctaataaaaa aacgcccact
     1021 agtgcgccgg ttgttggaga tataaaactc acgtcagcga gtctgggtgc tggtaatgtt
     1081 ctgattgatc gattatataa cgttaatggt attgctccac acagtataga agccccgata
     1141 atccatcccc acgaagacac gtttaatgca cctaactcac tcaaattacc actaaatatt
     1201 gcgaatacac caaacggtat tactgctagt gaatattcaa taaacccaag ttgttctcct
     1261 ggtatttttt tatattttgt taggagcttt ctaccaatta caattgcgga tgctgtcaag
     1321 aaagccataa agaataataa tatatctccg gttacagaag agactgtatc gttagtagca
     1381 gagactgatc ctagtataat tccgagcata cccattaccg caatcaccgc tccaacaatt
     1441 acacgtttat gcggttgttc tgataaaaat attgcagcca aaaagtatac aataaacgga
     1501 gtagacagat gaataacggc agcatgaata gcagtagttc tttgaatacc agcagaatat
     1561 aaaaaattaa ttatgactgt atctacaatg gtaaaaagtg caataagccc cagtactcgc
     1621 acagataacc gaacaaattt tttgcgttct gttgcataaa taaaaactgc acctaaaata
     1681 aatcgaaccg ccaataatac cgaaatagat actacagcta aagatttttt ataaaataat
     1741 gcagaaaacc cccataatac cccactcata aacaacattg agtatgcttg atgtgaataa
     1801 ctccgtactt tatgtttcat atttttgtat gtaggcttca atatcgaact tacgtttaag
     1861 cccattatat accattgatc gtactgttcc atataccggc cgttcgcccc aaggtctatc
     1921 gtgcagccct gcgatactcc aaagtgcacc aacataacca ttgggatctc cgccgtcaat
     1981 tgaataaaaa tcatttaagt atatgagttt ttgctgtgct tcttgcgcat cctttgacca
     2041 ctcaagtact ttttttgccc aatacatgcg catatagcca tgcatcttac cggtcgcagt
     2101 taactgtcgc tgagcagcat tccatgcaag gtcgtgcgta ttcgcagttt ctagctcttg
     2161 taggctatat atgtagtttc gtgggtctgt ttcgtgtttc tggagtgtac gacgtgacca
     2221 ttcaggcgct cctcgtagcg tcgtgtaatc ttggttatag ttgcaataat tatcagctaa
     2281 ttctttacgt attacgagct cttcttgtag tgcatcagcg tcattttgca acgctgcatt
     2341 agcaactacc gcagccgaaa ctgcattcat tacagataca ctccctatct gaccaaaatg
     2401 tagatacgga cttaggtctg atgtatgttg ctttgtagga tcatttctac tgtgtgcgta
     2461 gccatgtagc ttcgtgtcaa taaagtgctg taaatgctgc tgtgctgctg tttcacctgc
     2521 ggtgaaacca ctgtgagtat tgtttggtgt gtgctggtta atatataggg taagattttc
     2581 agctacttct tttagattca tgacagcacc cggccagcga tacggatgtt tttttatcgt
     2641 agaatgatga tgtataaaat cgttatagtg tgaatatatt ttaggacgta tcgtacgagc
     2701 actatattct tgtttattac tgacgagcca cgggggcaca atattatgag aatcaacaat
     2761 acatagtggc actgtttgag ataagacatt ttgccagcgt agtgtgccac gaagtggtga
     2821 ataatcagtt accatcagtg caggtttggt gtggtgtgcg atattcctta gcacaacatc
     2881 gtgcggacca aatagggcaa taaaaggtat cgcgagttta gctaatgttg tttctatctc
     2941 gcgcaagcct tcaatcatga atactacatg ctcgcgggcg cgacccttaa caggagaaaa
     3001 acaaaataca actgcgagtg gcagcttatg acgcagagca taatcttgcg cataagctaa
     3061 cccgaaattg tcggtaattc tttgatcacg actcattacg tagattactg attgaccgtt
     3121 tgcatttggt aaggtattga ttattttggt acgaagagct aactcactaa tattggattt
     3181 ttccacaact aggatctcca atttgtatgt ggtcgccata ttctatacct aatttaagta
     3241 gttgattcgt tatgcccatt tttttcaaaa tatcgcgtaa acgcatcacg ccaaagtcgt
     3301 tttcgtagtc tgtgcgtttt gcaaataatt ctattttctt accagtgact aagaatagcc
     3361 catctttcac cttacttgcg acccattgtg tatcgttttg ttctaatgtt attaacggta
     3421 gtgcatcttc atcggcgtga agctcgctct gtttctcgtc agcttctatt acgagttttg
     3481 ctgctttgtg caataattct ttgatttttg tatgggcatg cgcagataca acacatattg
     3541 tactaccgtc tggtattact tcactcagta attctttctg catcgcaatt atttcttcat
     3601 ctacaagttc tgcctttgta aatacaataa tttgctgttt ttgggcaagt tcttgattat
     3661 aggctgatag ttctttctgt atcatagtgt aatcggtagc gacatcattg ctcgtcacgt
     3721 caattaaatg caatagcact ttgcagcgcg aaacatggcg taaaaagtca tcaccaagac
     3781 ctttgccttt gcttgcccct tctataagcc ctggtatatc tgcgataagc agtgaatggc
     3841 tatctatgtc taccacaccg aggttaggca cagttgtcgt aaaaggataa ttggctattt
     3901 ctggcttggc attgctcaca acacttagga gcgtactctt accagcattt ggtaagccaa
     3961 tcaatccaat gtctgcgaca agtttcattt ctaatataac cgttcgttgc tcgccttgtt
     4021 cacccttttc tgctactcgt ggtgcttgtc gtacagaact cgtaaaatgt gcattaccaa
     4081 aaccgccaga gccaccctta gcaatgactg ctgcctgatt atcttctgtt aaatcagcaa
     4141 taacatcacc tgtttcggca ttcacaattg ttgtgcctat tggtacgagt acaacagtgt
     4201 ctttggcaga acgaccacgt tttttccgtt tacttcctgc ctggccatgt tctgcttgaa
     4261 tacgtttttg atacctaaac gtcgcaaggg tattttcatt tctcgttgcc tgcaatatga
     4321 catcgccacc cttaccgcca tctccgccat cagggccacc tctgtctaca aatttttcat
     4381 gtctaaaact aacgatgcca ttaccaccgt caccagccaa tacgtcaatc gtcactatat
     4441 ctacaaacat actgttacta gtgtaccaga taggctcaaa gaacagtgta ggcgaagcaa
     4501 atgaataagc tcttgtgagg ttgtgttgct agaattatcg gtaacttaac gatatatata
     4561 gttttggaac gtagacgttg gtacccaatc gctgtagcct actctaccaa agcctgcaat
     4621 accattcata tcagaatact tcatcatagt gccatcagct gaaacagcct ctacaacagc
     4681 aacgtgtccc caccaactca tggcatcggt ttgcgcaaca gcaccaggga cgggtgtttt
     4741 gctaacagtc cacccactag atcgggctct gttatcccac gtattggcat ttccccagtt
     4801 gctcggcact gcaaccctgt tggctgcata ccacgtacaa tagccgtaat aatagccatt
     4861 gccaccatac cgggctacaa agttatatgc aggagcagtg tttgaacttc tattagctgc
     4921 ggctgtagat gaggtacgtg acgcacgagg agttactggc ggttgaccac cggcgataaa
     4981 aatagtgtcg cctggcttta ggccagtgag ttctgtatcg ttgacggcta caagcttttc
     5041 tgcggaagct cggtactttg ctgccaaaga ttcaatagta tctccgtcag caacctgata
     5101 cacaatacca tcagatggtg gtatcttgag ctctttgcct gaaggtatgt cttcaattgc
     5161 tatgccattt gaccacttaa tagaatctgc cgaaactcca aattgagcgg cgattttagt
     5221 taccgtatcg ccttctacag caacatacgt agtaatatcg tttttagatt ctgaagcacc
     5281 agtcactact tgtggtttgg cgacaacaga ttcttctgta tttgcttgtg tgagctgagc
     5341 tttatatgaa tctgcttggt taataaccat cacttcttct ggcatactcg tcgccaatgc
     5401 aacatttgca gcaatatctg cagatgacag ggtgtcaagt ggtgcagatt cgtctgtgct
     5461 tgattgctgc ccaataaatg acaacgtagc acttgagcct tggctggca
//
LOCUS       DAOPLS010000031         5324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_16725_length_5324_cov_3.608654, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000031 DAOPLS010000000
VERSION     DAOPLS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16725_length_5324_cov_3.608654"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..693
                     /locus_tag="PKV52_01265"
     CDS             127..693
                     /locus_tag="PKV52_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HPD98876.1"
                     /translation="MTSAISLESAFKRAPRADFLPSNVKDDANIDAPLPIGFGQTNSQ
                     PSTVYNMLNWLQVEPGNNVLDVGAGSGWTTALLAYLVGKEGHVTATEIVPELATFGRH
                     NCQSLPLQNITFLTAKQQLGYKNNAPYDRILVSAAAKTIPEELVSQLSSDGIMVIPLQ
                     SSIMVMTKTNSTLTIDEHPGYAFVPLIN"
     gene            complement(690..2507)
                     /gene="typA"
                     /locus_tag="PKV52_01270"
     CDS             complement(690..2507)
                     /gene="typA"
                     /locus_tag="PKV52_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPD98877.1"
                     /translation="MHTPDKIRNIAVIAHVDHGKTTIVDGFLKQSHTFRDNQAEMQQT
                     LLMDSGDQERERGITITAKITAVHWNDYRINIIDTPGHADFSGEVERTLNMADGCLLI
                     VDAQEGPMPQTKFVLSKALQMGLKPIVIINKIDKPGSRIFEVKDELDNLFLELAVNED
                     QLHYPIYYSIGREGKAWDEMPEDTSVDANLNPIFDAIIADIPAPDVAPDKPFQLLVTA
                     LSWDNFQGKYAVGRVSRGSAVPGMPVALCKKNGSIVKGKIEKIYESQGLTKLVIPEAI
                     SGDIVSVTGVADAQIGETIADINHPEALPVIAIEPPTLQIYLGPNTSPFKGKEGDFTT
                     TRQISDRLQKELETNVGLRVEQEGLGFKVSGRGELHLSVLIEAMRREGYEFEVGRPQV
                     VTMNEDGVVKEPIEHVTIEVPSEHVGAVQMELGTRRAELQSQHTTSSGAMRLTYVMPT
                     RAMLGMRNVLLTSTKGTVIMNSISAGYEPLGADLRQLRNGVLIAWEQGTATPYALQTA
                     QERGTCFIGPGANVYAGQIIGLNTRSGDLEINVAKEKHLTNMRSSSSDGVVQLTPPVT
                     MSLEQCIDFIENDELLEVTPKNLRLRKKELNHNQRKRKT"
     gene            2650..3624
                     /locus_tag="PKV52_01275"
     CDS             2650..3624
                     /locus_tag="PKV52_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPD98878.1"
                     /translation="MQVVVFLLIAAVCSGLNVALMSLNVTDLRRLSALGNKQAKVVLP
                     LRQNRHLSLAGILFTNVAANSAVAIVLNQYLIGLIAGFLSTILLVLFSEIMPQALFMR
                     NALSVCYHLSWLIKLMIIITYPLSKPTQLLLDRLFRDSPGQLHSRRELGFIFNEHAYD
                     SRSELDDDEVEIIQNTLLLSEKRVSQIMTPIEHVYWLPYTAAITQQTLQNIIDKNFSR
                     VPVFNDTLTACYGVILMKDFVQIEENNLPISLQDIPLYQTKVIGSRTVLDTLFRKFIA
                     AKTHLIPIEKDNHIIGIVTIEDLLEEIIGHEIEDESDYKRNLATLNST"
     gene            3652..5172
                     /locus_tag="PKV52_01280"
     CDS             3652..5172
                     /locus_tag="PKV52_01280"
                     /inference="COORDINATES: protein motif:HMM:NF022794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HPD98879.1"
                     /translation="MLRKFYKNKFVFIPSVVLGVLILAYVSFGLWQYTTTSSQFAAST
                     TLYGINIGNQSVNDAKATVNTQLANSKVIITANDVTIEDTAANLGVYISDSQLSQALS
                     AQRLNRLVNPLFYNKYTAPLVSIDELQFQKSTLPAIPQDKQPPKNASFVVAEDQVTIQ
                     DAVSGNSILLSDVAQNIVNIVFNPTNANGTIQTTLKQVTPVLNTEILSKLKDKAQAIY
                     NNTYSLSDGTNNYEISKPRLITMLIPNSNYTELTLRESDSLILLEEAAAKANKPAVNE
                     ITTNYKSGKPQAVTTQGADGRNANNIGKIAQQLVTAVNQQTAFTSQLSFDTVPFQKKQ
                     ITVDDTVRSVTYTYRIITWGNTKSSLDDFAAKVAQTLADGRGWAQAGVTFARVSGASN
                     FDIVLSEPSELPARYPGTCDSTYSCRVGRYVIINDDRWRLATPSWNAAGGSLRDYQHM
                     VVNHEVGHRLGRGHEFCSAAGQPAPVMQQQSISLQGCTFNPWPLPYEIAAVQRSNR"
BASE COUNT         1562 a         1174 c         1040 g         1548 t
ORIGIN      
        1 aatacttatt agatagttat tttttagaag agtctgcccg ttttatttca agcaggcttt
       61 ctattgttag tatacaaaaa ttagcactct gctgtcaaga gtgctaattt gctgtatact
      121 aacagtatga catcagcaat atctttagaa tcagcattta agcgtgcgcc acgcgctgat
      181 tttttgccaa gtaacgtaaa agatgacgcc aatatagatg ctccattgcc tattgggttc
      241 ggacaaacaa atagccagcc aagcactgtt tacaatatgc tcaactggct acaggtagaa
      301 cctggtaata atgttttaga tgtaggcgca ggttcgggtt ggacaacagc tttactggca
      361 tacttagtcg gtaaagaagg acatgttact gcaacagaaa ttgtgccaga attagctact
      421 tttggtaggc acaattgtca aagcttgcca ctgcaaaata tcacattctt aactgcaaaa
      481 cagcaactag ggtataaaaa caatgcacca tacgacagaa tattggtgag cgcagctgcc
      541 aaaacaatac cagaagagtt agtaagccag ctgtctagtg acggcatcat ggtaatacca
      601 ctgcagagca gcattatggt aatgacaaaa actaacagta cactaacgat tgacgaacat
      661 ccagggtacg cttttgtacc ccttataaat taggttttgc gtttacgctg gttgtggttg
      721 agttcttttt tgcgcaatcg taggtttttt ggagttactt caagcagctc atcattttct
      781 ataaaatcaa tacactgttc taggctcatc gtaactggtg gggtaagctg tacaacgcca
      841 tcggacgaag aactacgcat attggtaaga tgtttttctt tggcaacatt aatctctaaa
      901 tcaccactgc gcgtattaag gccaataatt tgcccggcgt aaacattcgc gcccgggcct
      961 ataaagcagg tacctctttc ttgtgcagtt tgcagggcat acggtgttgc cgtgccctgt
     1021 tcccaggcta tcaatacacc attacgtagt tggcgcaaat ctgcacccag aggttcataa
     1081 ccagcactaa tactattcat aattacggtg ccttttgtgc tcgttaaaag cacgttgcgc
     1141 attccaagca tggcgcgtgt aggcattacg taggttagtc gcatggcgcc actacttgta
     1201 gtgtgttgtg attgtagctc agcgcgccgc gtgcctagtt ccatctgtac ggctccaaca
     1261 tgttcgcttg gcacttcaat tgtaacgtgt tcgatgggtt ctttaacgac accatcttcg
     1321 ttcatggtaa ctacctgtgg acgaccaacc tcgaactcgt agccttcacg acgcattgct
     1381 tctattaaaa cgctcaagtg gagttcacca cggccagata ccttaaaccc aagaccctct
     1441 tgttctaccc gaagaccgac attagtctcg agctcttttt gcaaccgatc actaatttgt
     1501 cgtgttgtcg taaaatcacc ttctttgcct ttaaatggac tcgtattggg ccctaggtat
     1561 atctgtaatg ttggaggttc tatggcaatg actggcaacg cttctgggtg gttaatatct
     1621 gctatggttt caccaatttg ggcatcagct acgcccgtta cacttacaat atctccagaa
     1681 atagcttctg gaatgacaag tttagtgagc ccttggcttt cgtatatttt ttctatttta
     1741 ccttttacaa tactaccgtt ttttttacag agagctacgg gcattccggg cacagcactt
     1801 cctctgctga ctcttcctac agcgtatttc ccctgaaaat tatcccatga tagagcagtt
     1861 actaaaagtt gaaatggttt atctggcgca acatctgggg caggaatatc ggcaataatt
     1921 gcatcaaaaa ttgggtttaa gttggcatca acactagtat cttctggcat ctcatcccac
     1981 gctttacctt cacgaccaat tgaataatat attgggtaat gtagctggtc ttcattgacg
     2041 gctagctcta agaataagtt atctagctcg tcttttactt caaagattcg gctaccaggt
     2101 ttatctattt tgttgattat tacaattggc tttaagccca tctgaagcgc tttagataat
     2161 acaaattttg tttgtggcat tgggccttct tgtgcgtcaa caataagcaa acagccatct
     2221 gccatgttaa gggtacgttc tacttcaccg ctaaaatcgg cgtgcccagg tgtatcaata
     2281 atatttatgc ggtaatcgtt ccaatgcaca gcagttattt tggcggtgat ggttatgcca
     2341 cgttctcgct cctggtcacc actatccatt agtagtgttt gttgcatctc tgcttggttg
     2401 tctctaaagg tgtgcgattg ttttaaaaaa ccatcaacaa ttgttgtttt gccgtgatct
     2461 acatgtgcaa taactgcaat atttctaatt ttatcaggtg tatgcatggt gcctttacaa
     2521 taaaaatgac cagtacgatt accggtcgct tatgaatata acaattatac catatttcgt
     2581 cacaatatct tatttgttca catatagtat tcgttttata gtaatgatat cgacatactt
     2641 tatattatca tccaagtagt agtattttta cttatagcag cggtttgttc ggggctaaac
     2701 gtcgcattaa tgtcgttaaa cgtaaccgat cttcgcaggc tcagtgcatt gggtaataaa
     2761 caagccaaag ttgttttacc gctgcggcaa aataggcacc ttagcttagc aggtatacta
     2821 tttaccaatg ttgctgccaa ttcggccgtc gcaattgtac taaaccaata ccttattggc
     2881 ctcatcgcag ggttcttaag tactattttg ctagtcttat ttagtgaaat aatgccgcaa
     2941 gcgctcttta tgcgtaatgc gttgtctgtt tgctaccact taagctggct tataaaactt
     3001 atgattataa tcacgtatcc gctttctaaa cctacccaac tactgctaga tcgtctgttt
     3061 cgtgattcac ccggacaact gcatagccgc agagaacttg gttttatttt taacgaacac
     3121 gcctacgatt ctcggagcga gctagatgac gacgaagtag aaatcataca aaatacccta
     3181 ctcctcagcg aaaaacgtgt cagccaaata atgactccaa tagaacacgt ctattggctg
     3241 ccatatactg ctgctattac gcagcaaaca cttcagaaca taatagataa aaactttagc
     3301 cgtgtacccg tttttaatga cacgcttacc gcgtgttatg gtgtaatcct tatgaaagat
     3361 tttgtacaaa tagaagaaaa taatttacca attagcttgc aagacattcc actctaccaa
     3421 acaaaagtaa ttggtagtcg cacggtgctt gatacattat ttagaaagtt tattgctgcc
     3481 aaaacacacc ttataccaat agaaaaagac aaccacatca taggtattgt tactatagaa
     3541 gatttactag aagaaataat cggccacgaa atagaagacg aatccgacta caaaagaaac
     3601 ctcgctacgt tgaattctac ctagtatctt attggtatat actagatgta aatgctgcga
     3661 aaattttaca agaataaatt cgtctttata ccaagcgtag tgcttggtgt acttattttg
     3721 gcatatgtga gttttggctt atggcaatac accaccacga gtagccaatt tgcagctagt
     3781 acaaccttat atggtataaa cattggtaac caatcagtca atgatgccaa agcaaccgta
     3841 aatacacagc ttgccaatag taaagttatc attactgcta atgatgtaac aatcgaagac
     3901 acggcagcaa atttaggagt atacattagt gatagccaac tttctcaagc actttctgca
     3961 caacgattaa atcggttggt taatccactt ttttacaata aatacacagc accactcgta
     4021 agcatagatg aattacagtt ccaaaaaagt accttaccag ctataccaca agacaaacaa
     4081 ccaccaaaaa acgcgagttt cgttgtagca gaagatcaag ttactattca agatgctgtt
     4141 tcaggcaaca gcatactact cagtgatgtc gctcaaaaca tagtaaacat cgtatttaac
     4201 cccacaaatg ctaacggcac aatccaaacg acgctcaagc aggtcacccc tgtccttaat
     4261 accgaaatcc tttctaagtt aaaagacaaa gcacaagcca tctacaacaa cacgtattcg
     4321 cttagtgatg gtacaaataa ttatgagatt tctaagcctc gtctcattac tatgctcatt
     4381 cccaatagca attatactga gctaacacta cgtgagagcg acagcctgat tctgttagaa
     4441 gaagctgccg ccaaagctaa caaaccagct gtcaacgaaa ttaccaccaa ctataaaagt
     4501 ggcaaaccac aagctgtgac cacccaaggt gccgacggta gaaatgccaa caatataggt
     4561 aaaatcgcac aacaattagt taccgccgta aaccagcaaa cggcgtttac atcgcaacta
     4621 tctttcgaca ctgtgccgtt tcaaaaaaag caaataacag tagacgatac cgttagatca
     4681 gtcacgtaca cgtacaggat tattacctgg ggcaacacta aatctagtct agatgacttt
     4741 gccgcaaaag tagcccaaac acttgccgat ggcagagggt gggcacaagc tggcgttacg
     4801 tttgcacgtg ttagtggagc atctaatttt gatattgtgc tttcagaacc ttcagaacta
     4861 cccgcacgct acccaggcac atgcgactct acctatagct gtagggtagg tcgatacgtt
     4921 attatcaatg acgacagatg gcgtttggcg acaccaagtt ggaacgcagc tggaggctca
     4981 ctccgtgact accaacacat ggttgtcaat cacgaagtcg gtcatcggct tggcagaggc
     5041 catgaattct gttctgcagc tggtcagcca gcaccggtta tgcaacaaca gtccattagc
     5101 ttacaaggtt gtacgtttaa cccgtggcca ttgccatacg aaatcgctgc ggtacaacgc
     5161 tctaatcgtt agatctctct taatgatacg ttagcgtaga aacaccgtat gtttatctat
     5221 aatagtgggg taaaataaca acatgtttgt acaagtgctt ataggtttta tggtgctgct
     5281 gggtacgtgg gctgtcgtta acatagtatt atggcgcact catc
//
LOCUS       DAOPLS010000032         5289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_16887_length_5289_cov_3.314673, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000032 DAOPLS010000000
VERSION     DAOPLS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16887_length_5289_cov_3.314673"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1105
                     /locus_tag="PKV52_01285"
     CDS             44..1105
                     /locus_tag="PKV52_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPD98880.1"
                     /translation="MSTKVTMDDLLTQHEVTALKAGDVVEATISTIKKHEIWLDLGPR
                     GIGVVMRREIGNQKLEEGQVVSASVVDPEMEEGHALLSLKKAVKDRGWDELARVAEAE
                     EGIEVTAYDANRGGLLIELEGIRGFMPVSQLSADHYPRVSGADKDEILQKLNALINKP
                     LRVRILDASRKDNKLIFSEKAAIKDDMQARFAELNVGDVVEGAVTGVIDFGAFVNVDG
                     IEGLIHISEISWERVEDPRKYVKNGEVIKAKVIAIDKDRLSLSIKQLSEDPWLHDVDQ
                     FKKGQTVEGTITRITPFGAFVQLTPAVEALVHVSEITNGEEDAVDPEKIFKLDEKKQF
                     KVLEINKEARKIALSLKDA"
     gene            1205..2974
                     /gene="infB"
                     /locus_tag="PKV52_01290"
     CDS             1205..2974
                     /gene="infB"
                     /locus_tag="PKV52_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPD98881.1"
                     /translation="MARTIEITEQITVGALADKLSLPASKLITELFKNGIMVTINETI
                     DAETAQIIKDELGLDIELVVKKVDVVAETLATRERKVSDKAVQRPPVVAVMGHVDHGK
                     TSLLDVIRSSHVVDKEAGGITQHISAYQVEHNKRKITFLDTPGHEAFAALREHGARLT
                     DVAIIVIAADDGIKPQTLEAIRFARKAGVHMVVAATKIDKPGANIELLKQQLAEQEML
                     PEEWGGNTIVVGVSSKTKEGLDKLLDMVLLVADIEELKADTDVPAEGLIIESHMEHGK
                     GPIAVALVEQGTLKRGDFVVAGDTYAKVRVLESTTHGVLQQAPPSTPVVIIGFKALPE
                     FGESFSTVSSEKEARNAAEAYHISHAASAMRSNMSSKDLIRLINQKTKQQELNIVVKA
                     DVQGSLTSVVDALKTLDTDEVAIRIVGSSVGSVTENDIRMAASSNAIIYGFHVSMPQS
                     VKQLASRDNVRVRLFKVIYELIDDTRAEMSNLLAPEEVITEQGRLVVRGVFKTTKTEL
                     ICGGEVTKGQLVIPAFANIYRDDTQLAKDLVVTNLKHGPTDTKLVSQGEMCGISIETT
                     ARLDIQEGDRIELYTSETHERTL"
     gene            3119..4744
                     /locus_tag="PKV52_01295"
     CDS             3119..4744
                     /locus_tag="PKV52_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98882.1"
                     /translation="MSETVNLETPPHNPEDSAGGVSPDEGVDQYGANEANSQEISIGN
                     TITILVDEALHQGTVVDIRTDAHGNVEKYVVQRLDQSDENRYLIAPETVVAENFISTT
                     KDNFETRIATKSEELKDNADKLAEMLYARSGRVVTAKNYTKEQIDESAKEYKEKLDTI
                     CAEFIESFPEDQQEQAQIILNDRIAEFKAEYKTEVSRRVTQLRAEKLETLGPVRKKML
                     DGWKKLSQFSRSDKKWKKFAGIGGKAAVFAPIGVVGALLTPVVGGTAVAVGAVAGATA
                     IARGVARFKLDQLSEESEMHETEAAATDIDEEVEASSGVDSEPTAAATELPDDPESLE
                     KAVQESHDRKKANQKRAALALAVAATAFIGTRLIGEWIHSSHTPAPKLEEGGETQPDN
                     SLIDEKSGEPTDQPSAVAPTDPTETGGPDTGDTIETSGPDAGDTTETGGGTNAPDQET
                     SDSGADSNAPNLTDAQHEFEKSGAKYPWDAAADVYGRENASDALFKAVKAANKDGLNV
                     KWHGSGENAWISVKGNSNGGYVIKQLAQYMPKK"
     gene            4792..5073
                     /locus_tag="PKV52_01300"
     CDS             4792..5073
                     /locus_tag="PKV52_01300"
                     /inference="COORDINATES: protein motif:HMM:NF039821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HPD98883.1"
                     /translation="MNIKEEIVKRVQTLNLPEAEQLPAVEFIRVNLLQRIGVRIAEDA
                     PEETLTEFSRLLDINESDALEYLDSKLPNFHRMVLEELDILVEEYALMS"
BASE COUNT         1797 a         1006 c         1249 g         1237 t
ORIGIN      
        1 attgttaacg aggctcagaa atatatctat aaggagtaaa tatatgtcaa ctaaggtaac
       61 catggacgac ctattaacgc agcacgaagt gactgcgctc aaagctggtg atgttgtaga
      121 agcaactatc agcaccataa aaaagcatga aatttggcta gacctaggcc cacgaggcat
      181 tggtgtagtc atgcgccgag aaattggtaa tcaaaaatta gaagaaggcc aagtggtaag
      241 tgccagtgtg gtagacccag aaatggaaga aggccacgca ctactcagcc ttaaaaaagc
      301 tgtcaaagac cgcggttggg atgaactcgc ccgcgttgca gaggcagaag aaggtataga
      361 agtaaccgca tacgatgcta atcgtggtgg tttactaata gagttagaag gtatacgtgg
      421 ctttatgcca gtaagccaac ttagtgccga tcactaccca cgggtgagtg gtgcagacaa
      481 agacgaaatc ttgcaaaagc ttaatgcact tattaataag ccactaagag tacgcatttt
      541 agatgctagc cgcaaagata acaaacttat ctttagcgaa aaagcagcta ttaaagacga
      601 catgcaagca cgttttgccg aactaaatgt tggcgacgtt gtagaaggag ccgtaacagg
      661 tgtcatagat tttggagcgt ttgttaatgt agacggtata gaagggctta ttcatatttc
      721 tgagatatct tgggaacgtg tagaagaccc acgtaaatac gttaaaaatg gcgaagttat
      781 taaagctaag gtgattgcca ttgataaaga ccggctgagt ctgagtatca aacagttatc
      841 tgaagacccg tggttacacg atgttgatca gttcaaaaaa ggccaaaccg tagaaggtac
      901 tattacacga attacaccat ttggtgcgtt tgtgcagctt acgcccgcgg tagaagcatt
      961 ggtacatgta tcagaaatta ccaatggcga agaagatgct gtagacccag aaaaaatctt
     1021 taaactagat gaaaagaaac agtttaaagt gttagagatt aacaaagagg ctcgcaaaat
     1081 tgcgttaagc ctcaaagatg cataacagct accctaacgt tatgtagcta aaacaacaac
     1141 aataattggg ggcgctacat tgagcgggcg ccccctgcgg gtaagaggaa ggagctcata
     1201 ggcaatggca cgaacgattg aaattaccga acaaattact gttggcgccc tcgctgataa
     1261 gctgtcttta ccagcatcta aactaattac agagctgttt aagaatggca tcatggtgac
     1321 tataaacgaa actatcgatg ctgaaacagc ccagataatt aaagatgaac taggcttaga
     1381 tatagagctt gttgtaaaaa aagtagacgt tgtggcagag acattggcaa cgcgcgaacg
     1441 caaagtatct gataaagcgg tgcaacgacc accagttgtg gccgtaatgg gccatgtaga
     1501 ccatggcaaa acaagcttgc tagacgttat tcgtagtagc catgtagtag ataaagaagc
     1561 aggtggtata acccaacaca tatctgcgta ccaagtagaa cataacaagc gtaaaattac
     1621 ctttttggac acgccagggc acgaagcctt tgccgctttg cgggaacacg gggctcgttt
     1681 aacagacgta gctattattg tgatagcagc cgacgatggc ataaaaccac aaacgctaga
     1741 ggcgatacgg tttgctcgta aagccggcgt gcatatggtt gtagccgcta ccaaaattga
     1801 caaaccgggc gcaaatatag agcttttaaa gcagcagctt gccgaacaag agatgttacc
     1861 agaagagtgg ggaggtaata ctattgttgt aggagtgtca tcaaagacta aagaaggcct
     1921 cgataaacta ctagacatgg tgctacttgt agcagacata gaagagctca aggcagatac
     1981 cgatgtacct gcagaaggct tgattataga atcgcacatg gaacatggca aggggccaat
     2041 tgctgttgcg cttgtagagc aaggtaccct aaagcgaggc gattttgtag tggctggcga
     2101 tacgtatgca aaagtacgtg ttctagaatc tacaacacat ggtgtattac aacaggcacc
     2161 tccatctaca ccagtagtca ttattggctt taaggcgctg ccagaatttg gcgaaagttt
     2221 tagtactgtt tcatctgaaa aagaagcaag gaatgctgca gaagcttacc atatatcgca
     2281 tgccgctagt gccatgcgta gcaacatgtc gagcaaagat ttaatacggc tgattaacca
     2341 aaaaaccaaa caacaagaac taaatattgt tgtaaaagca gacgtacaag ggtctttgac
     2401 atctgttgta gatgccctta aaacgctaga cacagacgag gttgcgatac gcattgttgg
     2461 atctagtgtt ggcagtgtaa cagaaaacga tatccgtatg gcagccagta gtaacgcaat
     2521 tatttatggt tttcatgtaa gcatgccaca aagcgtcaag caactcgcct cgcgcgataa
     2581 cgtacgggta agattgttta aggttatata tgaattaatt gacgataccc gcgccgaaat
     2641 gagtaacctc cttgcaccag aagaagtgat tacagaacag ggtagattag ttgttcgggg
     2701 agtatttaaa acaaccaaga ctgaactcat ttgtggtggt gaagtcacaa aaggccaact
     2761 tgttatacca gcctttgcca acatctatcg tgatgatacg cagcttgcca aagatttagt
     2821 tgtcaccaac ttaaagcatg gcccaaccga caccaagctc gttagtcagg gcgaaatgtg
     2881 tggcattagt atagaaacca ctgcgcgcct agatatacaa gaaggcgatc gtatagaact
     2941 ttacaccagc gaaacccacg aacgcaccct gtaaccaaca ttacacctat tatttgacta
     3001 ttgacatttt tatgttctta tgctagtatt tatttaaaca acaatagtaa tacgtaaata
     3061 acataaaaac ccataacact ccaaccgtaa gaaacctaag gaaaggtaat tattagccat
     3121 gtcagaaacg gtaaatttag aaactccacc acacaatcct gaagatagcg cagggggagt
     3181 atcgccagac gaaggagtag atcaatatgg agcaaacgag gcaaacagcc aagaaattag
     3241 tattggtaat actatcacga ttttagttga tgaagcccta catcaaggta ctgtagttga
     3301 tattcggaca gatgctcatg gaaatgttga aaagtatgtt gtgcagcgtc tggaccaaag
     3361 tgatgaaaat cggtatttaa tcgcaccaga aacagttgta gcagagaatt ttatctcaac
     3421 cacaaaagat aacttcgaaa caagaatagc tactaaatca gaagagctca aagataatgc
     3481 tgataaacta gcagaaatgc tctacgctcg gtctggccgt gttgtaaccg caaagaacta
     3541 cacaaaggaa caaatagacg aatccgcaaa agaatataag gaaaagctag atactatttg
     3601 tgctgaattt attgagagtt ttcctgaaga ccaacaagag caagctcaga ttattttaaa
     3661 tgaccgcatt gctgaattta aagctgaata taagacagaa gtaagccgta gggtaaccca
     3721 gttgcgagcc gaaaagttag aaaccttagg gcctgttcgt aaaaaaatgc ttgatggatg
     3781 gaaaaaacta tcacagttta gcagaagtga taaaaagtgg aagaagtttg ctggcatagg
     3841 tggtaaggcc gccgtgtttg ctcccatagg tgttgtaggt gcactattaa caccagttgt
     3901 tggaggcaca gctgttgcgg taggagccgt tgctggtgcg accgctatag ctcgtggtgt
     3961 ggcacggttt aagttagatc aactttcgga agaatcagaa atgcatgaga ccgaagccgc
     4021 tgccactgat attgacgaag aagtcgaagc aagttctggt gtagatagtg aaccaactgc
     4081 agcagctacc gaattgcctg atgatcctga atcattagaa aaagcagttc aagaatcgca
     4141 cgacagaaaa aaagcgaacc aaaagcgggc agcacttgct ttagctgttg cagccaccgc
     4201 atttattggt acgcgactca taggtgagtg gatacatagc agccataccc cagcaccaaa
     4261 actagaagaa ggcggtgaaa cacaaccaga caacagttta attgatgaaa aaagtggaga
     4321 gccaacagac cagccgagtg cagtagcacc gacagatcca acagaaaccg gtggtcctga
     4381 taccggcgac actatagaaa ctagtggtcc cgatgctggt gacacaacag aaactggtgg
     4441 tggaacgaat gcaccagatc aagaaacgag tgatagtggc gcagatagta acgcaccaaa
     4501 tcttactgac gcacaacatg aatttgaaaa aagtggtgct aaatatcctt gggatgcagc
     4561 cgctgacgta tatggcagag aaaacgcgtc agatgcatta tttaaagctg ttaaagcagc
     4621 caacaaagac gggcttaacg taaagtggca tggtagtggc gaaaatgctt ggatatcggt
     4681 taagggtaac tctaatggcg gctatgtcat taagcagtta gctcaataca tgcccaagaa
     4741 ataatacgcc gtttgatata cttagactat taaataatta aggacacaaa aatgaacatt
     4801 aaagaagaaa tagtaaaacg agtgcaaact cttaatctgc cagaagcaga gcaattaccg
     4861 gcagtagaat ttattagggt taacttattg caaagaatag gcgtaaggat tgcagaagat
     4921 gctccagaag aaacgctcac tgagtttagt aggcttctcg atatcaatga aagtgacgcg
     4981 ctagaatacc tagattcaaa attgccaaat tttcatcgga tggttttaga agagttggat
     5041 attcttgttg aagaatatgc attaatgagc tagacaccaa ggtgtaccac tgcggtatta
     5101 ctgctgtagt ggtatactac atataaataa ttagtaaaaa aggagacgta acctagcatg
     5161 aagatagaca atgattttat agcagaacta ggtttggcag cattgccaga ggcagaaaag
     5221 caagctttaa ttgctcaaat agtagaaaca cttgaaatgc gtgttggtac cgtattggcg
     5281 agtggctta
//
LOCUS       DAOPLS010000033         5228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_17173_length_5228_cov_3.675237, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000033 DAOPLS010000000
VERSION     DAOPLS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17173_length_5228_cov_3.675237"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKV52_01305"
     CDS             complement(<1..352)
                     /locus_tag="PKV52_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98884.1"
                     /translation="MLHITNKHHKKIILIAAAIVFAVSPVLVYVNHGAKAETSEELRN
                     RAKALEEQIKQNNAAAEQLANEADGLKRKIGELDAQITGVNAQIQLTNVKLQELEIKL
                     NDAQKELDRQKELLR"
     gene            complement(444..1382)
                     /locus_tag="PKV52_01310"
     CDS             complement(444..1382)
                     /locus_tag="PKV52_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPD98885.1"
                     /translation="MMRHLITLRRIFAAGIKNFFRNSWLSIAATAVMVVALVIVLMAV
                     VLNVVTRSAIAELSTNLKVSVYLQDNTDEALRKELQNEFFTNSNVDKVTFISKEEAQK
                     QFAASFQEDKKLLEGLALIGGNTLPASFEVGVTDLSKLEDVGNTAKQEKYASIVESVS
                     LGKTDVKKTIDRAANAQNFITSASIIAASIFAVVSMLIIFNTIRMAIFTRSEEIRTQK
                     LLGATPGYIRGPFLVESSMYGVIAGIVASSIVLAAIYSLGNKVSGQAEFIESYDFLTK
                     PSTIFAMYAASMLLGILVGIFSSTLAMGRYLKLKHW"
     gene            complement(1379..2074)
                     /gene="ftsE"
                     /locus_tag="PKV52_01315"
     CDS             complement(1379..2074)
                     /gene="ftsE"
                     /locus_tag="PKV52_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPD98886.1"
                     /translation="MRMILLDRVTKSYGRKSAALKGINLHVENKEFVVIVGQSGAGNS
                     TLLKLLTREEKPSSGKIIVGGIDYDKLKDKNVPYLRRKIGVVFQDFKLIQKKTVFENV
                     AYALEIVGMGNKEIKHTVPRVLNIVGLSNKADSYPSELSGGERQRVAIARAIARQPRI
                     LIADEPTGNLDPKHAWDVIEVLERINKYGTTVLLTTHNQDIVQKLNRRIVTIQDGKVV
                     SDKAGNARGRQTI"
     gene            complement(2278..3090)
                     /locus_tag="PKV52_01320"
     CDS             complement(2278..3090)
                     /locus_tag="PKV52_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD98887.1"
                     /translation="MARTQLQFEQKPAWVMSFVDSLVMIKRSSAHIVRNTDQLLGTFF
                     QPIMFLVLFSAVFGGAISLALPEGVSYLSFLMAGIIVQTVAFGSTTTAVAITNDLQKG
                     IVDRFRSLPMANSAVLNGHVVSDVFRNGISTAVMLVAGLALGFRSSASLLDWLLIAGI
                     LLLFTLSFSWLMAIVGVLAKSVEGVQWLSFVIIFPLTFASSAFVPTEGMNQYLKVFAE
                     NQPISQVIEAVRALMLGTPMGDHLFWAVFWCVLMLVIAVPIAINLFKKKTNN"
     gene            complement(3090..4049)
                     /locus_tag="PKV52_01325"
     CDS             complement(3090..4049)
                     /locus_tag="PKV52_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD98888.1"
                     /translation="MTKYAITVKKLKKAYKDNEVLKGVDLQVEHGTMLALLGPNGAGK
                     TTTVKILSTLLNSDEGQVTVGGFDVKRQPDKVRGVIGLTGQSAAVDELLTGKENLVMM
                     GRLYRLTKQSALARADELLQDFDLVEAANRPAKTYSGGMRRRLDLAVSLIATPPIIFL
                     DEPTTGLDPRSRIAMWNIITELKKQGTTILLTTQYLEEADQLADQIAVIDGGKVIAKG
                     TSAELKKAIGNDRLVLAFTQSNYVKAKKLFVSEKTMQHNDEDHALSFALKNTTKDTSV
                     ILQTLQKADIAVAAMDIHKPTLDDVFLSLTGKKQAKSTDKEQK"
     gene            4258..4704
                     /locus_tag="PKV52_01330"
     CDS             4258..4704
                     /locus_tag="PKV52_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98889.1"
                     /translation="MSERFNTHPHPARDGGDPIIGMGKFYLSEVQLGNLNEDSRAEYE
                     EALRRAEQSAREYGSLVSSAPKNPTSEIGRIGEKTSEINRLRIIHGMAITALAELIEG
                     LTFSGASSEEPIVLPPAPLRPDTAPEEPVIDLTKVDTPESAELINA"
     gene            complement(4764..>5228)
                     /locus_tag="PKV52_01335"
     CDS             complement(4764..>5228)
                     /locus_tag="PKV52_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPD98890.1"
                     /translation="SIFAKVDVLPKIDAPEEVAIDEKDLKVDVYRAGGHGGQSVNTTD
                     SAVRITHLPTGITVAIQNERSQLQNKETALTIVRSRLAQLQLEQHTQDIAKLKGPNEQ
                     AAWGNQIRNYVLHPYSMVKDTRTKYETSDVQSVLDGSINSLIEAYAEYAISS"
BASE COUNT         1441 a         1143 c         1026 g         1618 t
ORIGIN      
        1 cgcgcaataa ttctttttgc ctgtctagtt ctttttgggc atcatttaac ttaatttcta
       61 attcttggag tttgacattc gttagttgga tttgagcatt gacaccagta atttgcgcgt
      121 ctagttcacc aattttgcgc tttaagccat cggcttcgtt agcaagctgc tcggcggcgg
      181 cattgttttg cttaatctgc tcttctagtg ctttggcacg gttacggagt tcttcgctcg
      241 tttctgcctt ggcaccatga tttacgtata ccaaaacagg cgaaacagca aatacgattg
      301 ctgctgcgat taaaatgatt tttttatggt gtttgtttgt aatgtgcagc attgttacgc
      361 ttatgattat accactttca tagcataagc gtcaatacca gtgcgtacaa tcttaacacc
      421 tgtaagcgaa caaaatagaa aagttaccag tgcttgagtt ttaaataacg ccccatcgcc
      481 agcgtactag aaaatatacc gactaaaatg cccagtaaca tactggcggc gtacatggca
      541 aagatggtgc ttggctttgt taaaaagtca tagctctcta taaattcggc ttggccagat
      601 actttgttac ctaacgaata tatcgctgcc agtacaatac tagatgccac aatacctgct
      661 ataacaccat acatgctaga ttcaaccaaa aatgggccac gaatgtaacc gggtgtcgca
      721 cctaataatt tttgggtgcg gatctcttct gaacgggtaa aaatagccat acgaattgtg
      781 ttaaatataa taagcatgct cacgaccgca aaaatgctcg cagcaattat actggcagat
      841 gtgataaagt tctgggcgtt agctgccctg tctatggttt ttttaacatc tgttttacca
      901 aggctaacac tttctacaat actggcgtat ttttcttgct tagcggtatt gccgacgtct
      961 tctagcttag ataaatcggt cacaccaact tcaaacgatg ccggcaatgt gttaccacca
     1021 attaaggcca agccttctag taatttttta tcttcttgaa agctagccgc aaactgcttt
     1081 tgggcttctt ctttgctaat aaaggtcact ttatctacat tactattggt aaaaaattcg
     1141 ttttgcagtt ctttacgtaa cgcttcatcg gtattatctt gtaaatagac agacactttt
     1201 aagttggtag agagctctgc tattgcgcta cgtgttacaa catttaatac gactgccatc
     1261 agcacaatta ccagcgctac taccataacg gccgtcgctg caatagagag ccatgaattt
     1321 ctaaaaaaat tcttaattcc cgccgcaaaa atacgccgca aagttataag atgcctcatc
     1381 atatagtttg cctacccctt gcgttaccag ctttatcgct cactacttta ccgtcttgaa
     1441 tcgttacaat acggcggttg agcttttgga caatatcttg gttgtgggtg gtaagtagta
     1501 ccgttgtacc atatttatta atgcgttcga gcacttctat gacgtcccac gcgtgtttgg
     1561 ggtctaggtt accagtaggc tcatcggcta taagtatgcg cggttggcgg gcaatggcac
     1621 gggcaatagc aacacgttgg cgttcaccac cagaaagttc gcttgggtag ctatctgcct
     1681 tgttgcttaa gccaacaata ttgagcacgc ggggcacggt gtgtttgatt tctttgttac
     1741 ccataccaac aatttctagt gcgtacgcaa cgttttcaaa cactgttttc ttttgtatga
     1801 gtttaaaatc ctgaaacacc acaccaattt tacggcgtaa atacggcacg tttttgtctt
     1861 ttaatttatc gtaatctatg ccgccaacaa taatcttgcc agacgatggt ttttcttcgc
     1921 gcgttagtaa cttaagcaaa gtagaattgc ccgccccgct ctggccaaca ataacgacga
     1981 actctttgtt ttctacatgc aaattaatgc cttttaaggc tgcgctttta cgtccataac
     2041 tttttgttac cctatccagt aaaatcattc ttatggtaat tataccaaat ttgcgccata
     2101 catgttagag gctggtgttt gatggtaatt ctttaagtac ctgctcacgt aggttttata
     2161 gtgccagagt cttggttttt tgctggtaca gctttaataa aagcacggcg cattcaacca
     2221 catggtgccc aaccgcgcgc cctcatcaat atcaattttc tctaactcaa ccatatgtta
     2281 attgttggtt ttctttttga atagattgat agcaattgga actgctatca ccaacataag
     2341 tacacaccaa aatactgccc agaacaaatg gtcgcccatt ggcgtgccaa gcatcagtgc
     2401 tcgcaccgcc tctattactt gcgatatagg ttggttttct gcaaatacct ttaaatattg
     2461 gttcatgcct tctgttggta caaacgcgct actagcgaat gtaagcggaa atataatgac
     2521 aaagcttaac cattgtacgc cttctacgct ttttgcaagc acaccaacaa ttgccattag
     2581 ccaagaaaag cttagcgtaa acagcagtag tatgcctgca ataagtaacc aatcgagcag
     2641 cgatgcgctg cttctaaagc cgagtgccag gccagcaact aacataactg ctgtagatat
     2701 accgttacga aatacatctg acaccacgtg gccatttaac accgcgctgt ttgccatagg
     2761 cagtgaacgg aatctatcta caataccttt ttgtaaatcg ttcgtgattg ctactgctgt
     2821 agtggtagaa ccaaaagcaa ctgtttgcac aataatacct gccattaaaa agcttaggta
     2881 acttacgcct tctggtaaag cgagcgatat tgctccgcca aaaactgcag aaaataacac
     2941 caagaacata attggctgaa agaacgtgcc cagtagctga tcggtattac gcactatgtg
     3001 cgcgctacta cgcttaatca ttaccaatga atcgacaaat gacattaccc aggcgggttt
     3061 ttgctcaaac tggagctgtg tacgtgccat tacttctgct ccttatctgt agattttgct
     3121 tgttttttac cagtgaggct caaaaagacg tcgtcaagcg ttggtttatg aatatccata
     3181 gctgcaactg caatatctgc tttttgcaaa gtctgtaata taacagaagt atctttggtc
     3241 gtgtttttta acgcaaatga taacgcatgg tcttcatcat tatgttgcat agtcttttcg
     3301 ctaacaaata gctttttggc ttttacatag ttactttgtg taaaagcaag cactaatcgg
     3361 tcattaccaa ttgccttttt tagctccgct gacgtacctt tagcaataac cttaccacca
     3421 tctatgaccg caatctggtc tgctagttgg tcggcttctt ctaaatattg tgttgtcagc
     3481 agtatagtcg taccctgctt tttaagttct gtaatgatat tccacatagc aatgcgtgat
     3541 cgtggatcaa gccccgttgt aggttcatcc aaaaatatta tcggtggcgt tgcaataagg
     3601 ctaacggcta aatctaagcg ccgacgcata ccaccagaat atgttttggc tggccggtta
     3661 gctgcctcta ccaagtcaaa atcttgtagt agttcatctg cacgcgcaag tgcactctgc
     3721 ttagtgagcc ggtataaacg acccatcatt acaagattct ctttacctgt taataattcg
     3781 tctactgctg cagattgacc ggttaaacct attacaccac gtactttgtc tggctggcgc
     3841 ttaacatcaa aaccacctac agtaacctgt ccttcatcag aattaagtaa agtacttagg
     3901 atttttactg tggtcgtttt acctgcacca tttggcccaa gtagcgccag catcgtgccg
     3961 tgctctacct gtaaatcgac accttttaag acctcattat ctttataggc ttttttaagc
     4021 ttttttacag tgatagcgta tttagtcatg gtaatttagt aagtatacaa cataaagcca
     4081 ctcatttata tgaaatattt ctctttaaaa aacactacag gtggtgtatg ttttggttgc
     4141 atagccactt ctatgttgta caatgtagta tagtgtaatt ttgacaattt agcataagta
     4201 tgatacatta agtttatact gacaaaaatt aacaaaaaaa taggacaaaa aataaatatg
     4261 agcgagcgct ttaatacaca cccccaccct gcccgagatg gcggagatcc gattattggt
     4321 atggggaaat tctacttaag cgaagtacag ttgggtaatt taaatgaaga ttctcgagcc
     4381 gaatatgaag aagccctccg cagagctgaa caaagcgccc gagaatacgg tagtcttgta
     4441 agttctgcac cgaaaaaccc tactagtgag attgggcgta taggagaaaa aacgagtgaa
     4501 attaataggt taagaattat acatggaatg gcaatcacgg ccctcgctga gctcatagaa
     4561 ggcctcacat ttagtggagc aagtagcgaa gagcctattg tacttccacc agcaccccta
     4621 cgtccggata ctgcacccga agaaccggtt attgatttaa ctaaagttga cacacctgag
     4681 tcagccgagt taattaacgc ataagaaaca gcatacttag taaaatcgta acagatagat
     4741 ctctacatat agttaattta tgtttaacta ctgatggcat actctgcgta cgcttctata
     4801 agactgttta tagagccgtc taaaacactt tggacatctg acgtttcgta tttggtacgg
     4861 gtgtctttta ccatactgta gggatgtagc acataattac gtatttggtt gccccaggcg
     4921 gcttgttcgt ttggaccttt tagtttggca atgtcttgag tgtgttgttc gagctgtagt
     4981 tgcgccaaac gtgaacgcac aatcgtcagg gctgtttctt tattttgcaa ctgtgatcgt
     5041 tcattttgaa tagccaccgt tatgcctgtt ggtaggtgcg tgatacgaac agcactatct
     5101 gttgtattaa cactttgccc accatggcct cctgccctat agacatctac ttttaagtct
     5161 ttttcgtcta tagcaacttc ttctggtgca tctatttttg gcagtacgtc taccttggca
     5221 aaaataga
//
LOCUS       DAOPLS010000034         5189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_17348_length_5189_cov_4.309505, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000034 DAOPLS010000000
VERSION     DAOPLS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17348_length_5189_cov_4.309505"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1302)
                     /locus_tag="PKV52_01340"
     CDS             complement(277..1302)
                     /locus_tag="PKV52_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HPD98891.1"
                     /translation="MSAKIVVIGGGTGSFTVLSGLKKKFSDITAIVNMADDGGSTGQL
                     RDELGVLPPGDVRQCLVALSESPKLRELFTYRFEEGALKGHAFGNIFLTALEKMTGSF
                     ADAIDLADEVLQVNGRVVPVTLDNVRLIMRQRKGHDIIGEYKIGNMPFTLGEQRPILE
                     LSPPARLNPEAKRAIQAADMIVIAPGNLYGSVAPALLVKGMTRALQETKATIVYVCNL
                     VTKPGQTDGFFVHDFADEIERFIGARVLDFVLYNTQEPETSLRAKYIKDNEYNVAYDE
                     QILAQSHFTALGSAFLSGEAVQHSKADTIATSRSYIRHNTNAITQHLIAILERQKSNF
                     DNKYAQE"
     gene            complement(1409..1849)
                     /locus_tag="PKV52_01345"
     CDS             complement(1409..1849)
                     /locus_tag="PKV52_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98892.1"
                     /translation="MSIETIPPIGPNCVDTQDQTVVHEPIAPQRYEELDEVALYFLWE
                     NEEKRKKLERQRKELARWFKLEETASWEEINYHVELANAERLETVRLGRLALYRKERY
                     VVPGQEELDDRLSKILSDRFWTDQNPTRRLHLDQHASINHGQNY"
     gene            complement(2051..3289)
                     /locus_tag="PKV52_01350"
     CDS             complement(2051..3289)
                     /locus_tag="PKV52_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPD98893.1"
                     /translation="MSTHRKMYWGASTASHQVEGGTHNQWSVWELENANELAKTAKHR
                     LGWLANWQDIKSQASDPSNYISGEGVDHYTRYQEDFDLLQSLNLNAFRFGIEWARIEP
                     EQGVWDKAALAHYKQYIASLNQRGIEPFINLWHWTMPVWFTEMGGFEKRSNITYFEAF
                     VQKVSEELLHDVRYVITINEPNVYMGLSYSDGEWPPQKKSKLLAMYVYYNLAVAHKKA
                     YKIIKQKHPHMQIGVAQQLANIQAFRPGHWFDETVVKLMRYFWNWWWLNRTNKQQDFI
                     GFNYYFTDYYKGFRKQNPTKPINDLGWYMEPKGVLPLLQRIAYHYPKKPIFITENGVA
                     DAHDTYRQWWLQETMVAIETAKSQGIPVAGYLHWSLLDNFEWKYGWWPKFGLVAVDRK
                     TMKRTVRPSSKWWAHYIDKT"
     gene            complement(3757..5052)
                     /gene="rlmD"
                     /locus_tag="PKV52_01355"
     CDS             complement(3757..5052)
                     /gene="rlmD"
                     /locus_tag="PKV52_01355"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPD98894.1"
                     /translation="MRKRRVLPIESVHIDKLVHGGQGMGILADGRKCFVWGALPNEDV
                     RVQLTKRKKDWAEGVVEEIITASNDRIPPKEPDIYLATSPWQILHYKKEAIAKQAILE
                     ETYVREGVDVSWQPFYQQANPYHYRNKMEYNFWYDNDSQQVSLALHKRGSHQKIAVTG
                     SALASETINKAGAELIAYINAHAIQARPLKSVIIRSTATGICGATIFVNDKNVAKMFD
                     NYKPANTIVEIVYSNPNSPASISTEVVTPAEQQLSDTLLGNTFSYSARSFFQVNVPVY
                     EKLLQIVKGFVQKNSLLHILDLYSGVGSLGLSVLGKSQQLTMVEIDDLSCELAQQNSR
                     IVKNSAVVLATAENSLHYITGQELVIVDPPRAGLHKDVVARLIETKPATILYVSCNPS
                     TQARDVKALIEVGYTIHYAQGFNFFPRTPHIESFVVLNR"
BASE COUNT         1329 a         1182 c         1021 g         1657 t
ORIGIN      
        1 tgttcgcaca acactacata tagcgcacgt aataataaaa aagacactct tcttaaacca
       61 aagtgtcttt tttatgaatg gttggcttta ttcagtaccg cggacaaaga ctccttcgta
      121 gccaaagtct gggtctagtt aagtacgcca gtctccccta gccctttaac aacacggaat
      181 ccttcgtgta tttgatcttc acgagctgct gttggaccaa attcttgtga tatacgcatt
      241 ttttgtctct ttttgttaca ttgttctcta tatagtttac tcctgcgcat atttgttgtc
      301 aaagttactt ttttgacgct ctagtattgc aatgaggtgc tgtgtaatcg cattggtgtt
      361 gtgccgaata tatgatctac tggtagcaat tgtatccgcc ttactgtgct gcacagcttc
      421 accgctcaaa aaggcagaac caagtgccgt aaaatggctt tgtgcgagta tctgctcatc
      481 gtaagcaacg ttatactcat tatcttttat gtactttgcg cgtagcgacg tttctggctc
      541 ctgtgtgtta tataaaacaa agtctagcac tcgcgcccca ataaagcgct ctatttcatc
      601 cgcaaaatca tgcacaaaaa agccatctgt ttggccaggt ttcgttacga ggttacagac
      661 gtatacaatt gtagcctttg tttcttgtaa ggcgcgtgtc atgcccttca ctaacaatgc
      721 aggcgccaca cttccgtata aattgcccgg agcaattaca atcatatcgg cggcctgtat
      781 ggcacgcttg gcttctgggt ttaatcgtgc cggtggtgac agttctaaaa ttggccgctg
      841 ttcaccaagc gtaaacggca tattgcctat tttatattcg ccaataatat cgtggccttt
      901 acgttgccgc ataataagac gaacattatc taacgttact ggcactaccc taccattcac
      961 ttgcaacact tcatcagcca gatcaatagc gtctgcaaaa ctacctgtca ttttttctag
     1021 tgctgttaag aaaatattgc caaaagcatg gccctttagt gcgccttctt caaatctata
     1081 tgtaaataac tcgcgcaatt taggtgattc agataacgcc accaaacatt ggcgcacatc
     1141 accaggcggt aagacgccta gctcatcacg taattgccca gtagagcctc cgtcgtctgc
     1201 catatttaca atagcggtta tatcagaaaa cttctttttc agaccgctca gcaccgtaaa
     1261 gctacccgtt ccaccaccaa tcaccacaat tttcgcactc atagaattca acatactact
     1321 taattctact agatataccg ttatgtacaa ttatgaaact ttgtatatgc aaaaaatata
     1381 gtgtaagcgc ttagtctaaa gtgtattatt agtaattctg tccgtgattt attgatgcgt
     1441 gttgatctag atggagccgt ctggttgggt tctgatctgt ccagaatctg tctgatagga
     1501 ttttactcag acgatcatca agttcttctt ggccgggtac cacataacgt tctttacgat
     1561 aaagagctaa cctacctaat cttacagttt ctaaacgttc tgcgttggcg agttctacgt
     1621 ggtaatttat ttcttcccag ctcgcggttt cttctagctt aaaccaccta gccagctctt
     1681 ttctctgtct ctctagcttt ttcctctttt cttcattttc ccaaagaaaa tacagcgcaa
     1741 cttcatccaa ctcttcgtat cgttgcggtg ctataggctc gtgtaccaca gtctggtctt
     1801 gtgtatcaac acaattaggt ccaattggtg gtattgtttc gatagacatt tttgttacct
     1861 ttcgcttatg ccctaggtat actccccgta cattagtttg tcaatagcac gcttcgctgt
     1921 gtaagctgcc ggcagcatct tgtatcccct aagctaacgt agctgtagca aaattataaa
     1981 atatgcactg cagccaatag tgtgtgagcc ataacagccc tacctctcta taattattat
     2041 actctattag ctatgttttg tcaatatagt gcgcccacca tttactgctt ggccggacgg
     2101 ttcgtttcat tgtttttctg tctactgcca caaggccaaa ttttggccac cacccgtact
     2161 tccattcaaa gttatctagt aaactccaat gtaaatagcc tgccactgga ataccttggc
     2221 tttttgccgt ttctatcgct accattgttt cttgtaacca ccactgtcta tatgtatcat
     2281 gagcatctgc cacaccgttt tctgtaataa aaataggctt tttggggtaa tgatacgcaa
     2341 tgcgctgcaa aagtggcagt acgccctttg gctccatgta ccagcctaaa tcattgattg
     2401 gttttgtggg gttttgctta cgaaagccct tgtaataatc ggtaaaatag taattaaacc
     2461 ctataaaatc ttgttgcttg tttgttctat taagccacca ccagttccaa aagtaccgca
     2521 ttagttttac gacggtttca tcaaaccaat ggccaggtct aaacgcttgt atgttcgcca
     2581 gctgttgggc gacgccaatc tgcatatgtg ggtgtttttg cttgattatt ttatatgctt
     2641 ttttgtgagc aactgctaaa ttgtaataca catacatcgc aagtagctta ctcttttttt
     2701 gtggtggcca ttcgccatcg ctatagctaa gccccatgta gacgttcggc tcattgattg
     2761 ttataacgta ccgaacgtca tgtagcaatt cttcagacac tttttgtaca aaagcttcaa
     2821 agtatgtaat gttacttcgt ttttcaaagc cccccatttc tgtaaaccac acgggcatag
     2881 tccagtgcca caggttaata aaaggctcta ttccacgttg gttcaaactg gcgatgtatt
     2941 gtttgtaatg cgcaagagca gctttgtccc acacaccttg ttcgggttcg atgcgtgccc
     3001 attcaatgcc aaatctaaat gcgtttaaat tgagtgattg taatagatca aagtcttctt
     3061 ggtagcgcgt atagtggtct acaccttcac cggaaatgta attacttggg tccgaagctt
     3121 gagatttgat atcctgccag ttggctagcc agccgagtct gtgtttggcc gttttagcta
     3181 gttcatttgc gttttctaac tcccacacgc tccactgatt atgtgttccg ccttctactt
     3241 gatggctcgc ggtgcttgcg ccccagtaca tttttctgtg tgtgctcatt accgtatagt
     3301 atacatctaa acaacttagc cgtggtagca ctattggttt gatgcaaata tagtgctatg
     3361 aaaaaaaccg caccgggcat attcttggta aagaatttta gttgcctatc tgtgtgcggt
     3421 cacaagcggc gagaacctgg ctactagtat taagagccgc ggttgtcgcc aaaccaaaag
     3481 atgtgttcgg gtggagcggg caatacacgt gttgtactgc ccgctccatg gtgtgttttt
     3541 acttttttac tacttggtac aggtcggcgt agtgaaccgc cgcctgctgg cgacgctcac
     3601 tgtgagacgt agtgaacgcc tgcgccactg cgtagagtgc gagcaaaaac agtatgataa
     3661 ccaggaaagt catcattatt tcaccccttt caggtcatac taattatagc atattcggta
     3721 tagtatagac aacactgact ctagagcgaa caattattat ctatttagta ccacaaatga
     3781 ttcaatatgt ggtgttctag ggaagaagtt aaagccttgg gcatagtgta tggtgtagcc
     3841 aacctcaatc agtgccttca catcccgtgc ttgtgtgctg gggttgcagc tcacatacaa
     3901 aatagtggct ggtttggttt cgatcagtcg cgctactaca tctttatgta ggcctgctct
     3961 tggcgggtct acaatcacaa gttcttggcc tgttatatag tgcaaagaat tttcggctgt
     4021 tgccaaaaca accgcactgt ttttaactat gcgactgttt tgctgtgcaa gctcgcacga
     4081 caggtcgtca atttctacca ttgtgagttg ctgtgactta cccaaaacgc tcagtcctag
     4141 gctccctact ccactatata aatctaaaat atggagcaaa gaattttttt gcacaaaacc
     4201 cttaactatc tgtagcaatt tttcatatac tggcacattt acctgaaaga aagagcgggc
     4261 cgaataggaa aaagtattac ccaatagtgt gtcagataat tgttgttcgg caggtgtaac
     4321 tacttctgta gaaatacttg cagggctatt tggatttgaa tacacaattt ctacaatagt
     4381 gttagcaggt ttataattat caaacatttt tgctacattt ttgtcattta caaatatcgt
     4441 ggcaccacat atgcccgttg ctgtgctacg aataattacg ctttttaacg gccgtgcctg
     4501 tatggcgtgt gcgttaatat aggcaatgag ttctgcaccc gccttgttaa tagtttccga
     4561 cgcaagcgcg ctaccagtaa cggcaatttt ttggtggcta ccacgcttat gcagtgccag
     4621 acttacctgc tgtgaatcgt tatcgtacca aaagttatat tccattttat tacgataatg
     4681 gtacgggttg gcttgttggt aaaacggctg ccaactaaca tcaacacctt cacgcacata
     4741 ggtttcttct aaaatagctt gtttggcaat tgcctctttt ttataatgta aaatctgcca
     4801 cggactggtt gccaagtaga tatctggttc tttaggcggt attctatcgt tgctggctgt
     4861 aataatttct tcgacaacac cttctgccca atcttttttg cgtttggtaa gttgcactcg
     4921 tacgtcttcg tttggcaagg caccccatac aaaacatttg cgaccgtctg ccaatatgcc
     4981 cataccttgt ccgccatgaa caagcttatc tatatgaaca gactctattg gtaatactct
     5041 tcgttttctc ataaatactc tcgtcattat accaatgaat gcatcctaaa acgaacgcat
     5101 accaaaaagc tataacagat gttttttaag tgcgtattga ctggcgatgt agccgggtat
     5161 aagcggtagc caaaagataa caaatctat
//
LOCUS       DAOPLS010000035         5112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_17665_length_5112_cov_4.769231, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000035 DAOPLS010000000
VERSION     DAOPLS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17665_length_5112_cov_4.769231"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKV52_01360"
     CDS             <1..353
                     /locus_tag="PKV52_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPD98895.1"
                     /translation="YSENNQNIIGTLYLRDAIRLQKAKPVTKIMRDDVYYINEAQPLD
                     VALHGFIKTRHHLFVVVNEFEDVVGVLTIEDVIEQIIGHPIHDEDDRFDDLRLVAKSK
                     AATKRSSRKSNIIS"
     gene            398..2152
                     /gene="aspS"
                     /locus_tag="PKV52_01365"
     CDS             398..2152
                     /gene="aspS"
                     /locus_tag="PKV52_01365"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003140471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPD98896.1"
                     /translation="MRTLVTETINSIGKSVVVQGWVNSRRDHGGLVFVDLRDHTGLLQ
                     LTIDATKEGFHAAEELRDEYVIEATGLVKERDEHLKNNNIPTGGIELVVENLRILNKS
                     EPLPILVNTNQKVNEEHRLKYRYLDLRRQKMQDMLTKRAQYYATIRQYMETNAFTEIA
                     TPILANSSPEGARDFLVPSRVHSGKFYALPQAPQQFKQLLMVGGVNRYYQIATCFRDE
                     DPRADRLYGDFYQLDLEMAFVEDGETVRQTVEQLIIDLVTTFAGKSLAESKIPRITYQ
                     EALDTYGSDKPDLRFDMHLVDLTKELESCGFGVFTNAIKANGVVKAICAKSQFSRSQI
                     DTFTDIAKQNGAGGLPYMTVEDGKLHSSIAKFFSDEELNTIQKTTHAQDGDTIFFGAD
                     RKTVVNAALGVLRSHLGDVLGLKDDNTVALAWIVDFPFYEYDEKTKKLDFGHNPFSMP
                     KGGLGTLENEDKLSIVADQFDMVMNGYEICSGAVRNHNPEVMYKVFDVLGYKKSYVES
                     KFGAMLQAFKYGAPPHAGCAFGLDRIFMVLTNEDNIREVVAFPKNGSGVDVMMGSPST
                     VDETQLEELNIRIIESKD"
     gene            2167..2685
                     /locus_tag="PKV52_01370"
     CDS             2167..2685
                     /locus_tag="PKV52_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD98897.1"
                     /translation="MAHIHTLSGQHDFTVSVYIVRQFDDIDWRIALHVHKKHNSLLPF
                     GGHIELDETPWQAVARELKEESGYDMSSLSIVLSKPKLGLVGCDEHFVPFSVNTHAVP
                     GDVPHYHTDLTYAFVAKEEPTNAIADDESSDIRWLSLQEVEELSEKEIYQDARQLSVY
                     VLQSVQNMPAQI"
     gene            2732..4663
                     /locus_tag="PKV52_01375"
     CDS             2732..4663
                     /locus_tag="PKV52_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98898.1"
                     /translation="MDKNKDNTFDVPAAEIQDKITLLMGLPPEEATARLDKNSAPRRI
                     SSRRYKSTDDTSKSAEKVTDDPPADTKPKPLFDEAGNETDDTVVDEVIAQEHNTNTES
                     IAASSSDYTITPPKQNGIIRFAKAWWNHRILRTVTLLAVFGAVVFIVAFPQARYYVLN
                     TVGVRASAQFRVVDVISGRPIKNVTVSIDDKTAKSDEEGLVKINDIKLGPTLINLQKR
                     SFKEEQIPVTIGWGSNPFDGPLELNPTGTTFVFTAKDWLSGSALQNVEVSDGESVALS
                     DENGLAKLTIEPTEKDIAVTFTYNTYRTETLTIPADTIANEEVLLVPSSQDVFISKRS
                     GKYDIYSRYVDGTQETLLLPGTGTEQSDTHLLPNPKSSVVALVSSRDGLRNESGSMTS
                     SVYLLDTTTKQVEKIDTSSSERVRLIGWDGDTVVFVKTATNQLADKGSTQTIVAYDAA
                     KKEVHEVATASNFTDVRLIKDAVYYVLPVSDGSQSKGLTKSSLNNNEKKVLLAKNIWA
                     VYRITNDTLQISAEGDEWYELTLENGSIKQLEGAPAVPKNITYTSNPITTDVAWLEER
                     DGKTVLLVSSSNANDSKEITKQSGINYPIRWLNSTTILYTVATAGETADYIVSTTNGQ
                     VKKVGDVTLSVYADSQYYY"
     gene            4669..>5112
                     /locus_tag="PKV52_01380"
     CDS             4669..>5112
                     /locus_tag="PKV52_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPD98899.1"
                     /translation="MHNYSLFIDKVWQFYDTQKRDLPWRHINELTALDRGYRVLVSEM
                     MLQQTQVSRVVPKYHSWLEQWPTLADFMEATLADAIVAWSGLGYNRRAKYLFESLHAV
                     FAQYNGIVPPDVRALTKLPGLGTNTAAAVFVYTHNLPVAFIETNIR"
BASE COUNT         1747 a          926 c         1095 g         1344 t
ORIGIN      
        1 tatatagtga aaacaatcaa aatattatag gtacactata tttacgtgac gcaatacgtt
       61 tacagaaagc caagccagtt acaaaaatta tgcgtgatga tgtgtattac atcaatgaag
      121 cgcagccact cgatgttgca ttgcatggtt ttataaaaac tcggcatcat ttatttgtag
      181 tggtaaatga gtttgaggac gtggttggtg tactaacgat agaagatgtg atagaacaaa
      241 ttataggaca tcctattcac gatgaagatg ataggtttga tgacttgcgc ttagtcgcaa
      301 agagcaaagc agccacaaaa cgctcgtcaa gaaagtcaaa tatcatttct tagttaccat
      361 acatagagtc gtaccatatc tgttataatg acaagttatg agaacattag taactgaaac
      421 gattaatagt ataggtaagt ctgtcgtagt acagggatgg gtgaacagca ggcgtgacca
      481 tggtgggctt gtgtttgtag atttaagaga tcacacaggt ttattacaac ttaccataga
      541 cgctacaaaa gaaggctttc atgcggcaga agaactaagg gatgaatatg tcatagaagc
      601 aacgggtctg gtaaaagaaa gagatgaaca tcttaaaaat aacaatattc caacgggcgg
      661 cattgaactc gtggttgaaa acttacgaat ccttaataag agcgagccat taccgatatt
      721 ggttaacacg aaccaaaagg taaatgaaga gcataggcta aagtatcgat acttagattt
      781 gcgcagacaa aaaatgcaag acatgttaac aaagcgtgca caatactatg caacgattag
      841 acagtacatg gaaaccaatg catttactga aatagcgaca ccaatattgg ctaattccag
      901 ccccgaagga gctcgagact ttcttgtacc gtcacgcgta catagtggta agttttatgc
      961 attaccacaa gctcctcagc agtttaagca actactcatg gttggtgggg taaatagata
     1021 ttatcagatt gccacttgtt ttcgcgacga agatccaaga gcagacagac tctatggaga
     1081 tttttatcaa ctagatctag aaatggcctt tgtagaagac ggcgagacgg tacggcaaac
     1141 tgttgagcaa cttattatag atctggttac gacatttgcg ggtaaatcat tggctgaatc
     1201 taaaattcca agaattacgt atcaagaagc acttgataca tatgggagtg ataagccaga
     1261 cctcagattt gatatgcatc tcgtagatct aacaaaagaa ctagaaagtt gtggttttgg
     1321 tgtgtttacc aacgctatca aagcaaacgg agttgttaaa gctatttgtg caaaatcaca
     1381 atttagcaga tcacaaattg atacgttcac tgatattgcc aaacaaaacg gagcaggcgg
     1441 gctgccttat atgactgtag aagatggaaa actacactca tctattgcaa aattctttag
     1501 cgatgaagag cttaatacaa tacaaaaaac tactcatgca caagacggag atactatttt
     1561 ctttggtgca gacagaaaga ctgtagtaaa tgcagcgctc ggagttctaa gatcacacct
     1621 aggagatgtt cttgggttaa aagatgataa tacagttgca ttagcttgga ttgttgactt
     1681 tcctttttat gaatacgatg aaaaaacgaa aaaattagac tttggacaca atccgttttc
     1741 tatgccaaaa ggagggcttg ggacgctgga aaatgaagac aaactatcca ttgtggctga
     1801 tcaatttgat atggtaatga atggatatga aatttgttca ggtgccgtaa ggaatcataa
     1861 tccagaagtc atgtacaaag tgtttgatgt gctgggctac aaaaaatcat atgtagaatc
     1921 aaaatttggc gcgatgttac aggcttttaa atatggagca ccacctcacg cagggtgtgc
     1981 tttcggatta gacagaatat ttatggtgct gacgaatgaa gacaatatta gagaagttgt
     2041 tgcatttcca aaaaatggct ctggtgtaga tgttatgatg ggatccccga gcactgtaga
     2101 tgaaacgcaa ctagaagaac ttaatattcg cataatagaa agcaaagatt aggtatttac
     2161 cgcccaatgg cacatattca cactctaagt ggccaacatg attttacagt gagcgtatat
     2221 atcgtgagac aatttgatga tatagactgg cgaatcgcat tacacgtaca caaaaaacat
     2281 aattcgttgc ttccttttgg tgggcatata gaactagacg aaacgccatg gcaggcggtc
     2341 gcgagagaac taaaagaaga atcagggtat gatatgagtt cgctgtccat tgttctttct
     2401 aagcctaaac taggactcgt aggttgcgat gaacattttg taccgttttc tgttaatact
     2461 catgctgtcc caggtgacgt acctcattac catactgatc ttacctatgc atttgttgca
     2521 aaagaagaac ctactaacgc aattgctgat gacgaatcta gtgatatacg atggctaagc
     2581 ctacaagaag tagaagagtt atcagaaaaa gaaatctatc aagatgcaag acagttaagc
     2641 gtatatgtgc ttcaatctgt gcaaaacatg ccagcacaga tatagagatg ttcaaactaa
     2701 gctataaaat ggtaaaatat caatagttct tatggataaa aataaagaca atacatttga
     2761 tgtaccagca gcggagatac aagacaagat aactttgctg atgggtttgc cgccagaaga
     2821 agctacggca agacttgata aaaattctgc tcctcgcaga atatcttcac gtcgttataa
     2881 aagtactgat gacacatcta aaagcgcaga aaaagtaacc gatgatccac ctgcagacac
     2941 aaagcccaag ccgctctttg acgaagctgg caacgaaacg gacgatacgg ttgttgacga
     3001 agttattgct caggaacata acacaaacac ggaatctata gctgcaagct catcagatta
     3061 tacgattact ccgcctaaac aaaacggtat tatccgtttt gctaaagctt ggtggaacca
     3121 tagaatttta agaacagtaa cactgttggc agtgtttgga gcggtggtct ttatagtagc
     3181 ttttccacaa gcacggtact acgttttaaa cacagttggc gtgagagcaa gtgcacagtt
     3241 tagagtagtc gatgtaataa gtggtaggcc aattaaaaac gtaacagtaa gtattgatga
     3301 taagactgct aaaagcgatg aagaaggtct agtaaagatt aatgatatca aattaggtcc
     3361 aacattaatt aatttacaaa aacgttcttt caaagaagag caaattccag taacaattgg
     3421 gtggggcagc aacccttttg atggaccttt agagctgaat cctactggta ccacgtttgt
     3481 ttttacagcc aaagattggt taagtggttc tgctttgcaa aatgttgaag tttcagatgg
     3541 tgaaagtgtg gcactttctg acgaaaacgg acttgcaaaa cttacaatag agccaacaga
     3601 aaaagatatt gccgttacat ttacctataa tacatataga accgaaacac tcaccatacc
     3661 ggctgacact atcgcaaatg aagaggtact acttgtacca agcagccaag atgtatttat
     3721 atcaaaaaga agtggcaaat atgatatata tagtcgctat gtagacggaa ctcaagaaac
     3781 cttattattg ccaggtacgg gtactgagca atccgatacc catttgcttc ctaatccaaa
     3841 aagttcagtc gtcgcattag tgtcttctag ggacggttta cgcaacgaat caggcagtat
     3901 gacaagtagc gtatatcttt tagatactac aacgaaacaa gtagaaaaaa tagatacaag
     3961 cagtagcgag cgtgttcgtt tgattggctg ggatggtgac actgtagttt ttgttaaaac
     4021 agctacgaat cagctagcag acaagggtag cacacaaaca atcgtagcct atgatgctgc
     4081 aaaaaaagag gtacatgaag ttgcaactgc tagtaacttt acagatgttc ggcttattaa
     4141 agacgcagtg tactatgtgc ttcctgtcag tgatggctcc caaagcaaag gtttgacaaa
     4201 atcgagcctc aacaataatg aaaaaaaagt gctactcgcg aagaacattt gggcggtata
     4261 tagaataacc aacgatactc tacaaataag cgcagaaggt gatgaatggt acgaactaac
     4321 ccttgaaaat ggatcaataa agcaactcga aggtgcgccg gctgttccaa aaaatattac
     4381 ctatacgagt aatccgataa ctacagacgt agcatggcta gaagaaagag acggcaagac
     4441 agtacttttg gtttctagta gtaatgcaaa tgattccaaa gaaataacca agcaatcagg
     4501 cataaactac ccaattcgtt ggttaaatag cacaacgata ctctacacag tagccacagc
     4561 cggtgaaaca gcagattaca ttgtcagcac aacaaatggc caggtaaaaa aagtaggtga
     4621 tgttaccctt tctgtgtatg ccgatagtca atactactat taaaatctat gcataattat
     4681 agtcttttta tcgataaagt ttggcagttt tacgataccc aaaaacgtga tttgccatgg
     4741 aggcacatta acgaactaac agccctagat agaggttaca gggttttagt atcagaaatg
     4801 atgctgcagc aaacgcaagt cagccgtgta gtgccaaaat accacagctg gttagagcaa
     4861 tggcctacgc ttgctgattt tatggaagca acacttgccg atgcaatagt agcttggagc
     4921 gggcttggtt ataaccgtcg ggcaaagtat ttgtttgaat ctcttcatgc cgtttttgcg
     4981 caatataatg gaatagtgcc gccagacgta cgagctctta ccaaactacc aggtctaggt
     5041 acaaatactg cagctgcagt atttgtttat acccataacc tacccgttgc gtttattgaa
     5101 actaacattc ga
//
LOCUS       DAOPLS010000036         5092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_17765_length_5092_cov_3.463768, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000036 DAOPLS010000000
VERSION     DAOPLS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17765_length_5092_cov_3.463768"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKV52_01385"
     CDS             <1..789
                     /locus_tag="PKV52_01385"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPD98900.1"
                     /translation="VGANTLVVASSYSGNTEETLSALAQAEEKGAKIAVIASGGTLAH
                     IAQEKNYPFLQLPQVSQPRFAALYGLNALVTLLASGGIVPQTAVQELQGNAEWLKQHV
                     LSWRPDVPTQDNYAKQIALDAIGTSPVIYSSTELFPAAYKWKISFNENAKNVAWCNAY
                     PEFNHNEFLGWSSHPIDKPYTVIDLRSNLDHPRVQKRFALSAKLISGKRPAPIVIDVV
                     GETHLQQLLWAVALGDFVSLYTGLLNGLNPTPVDLIEKFKKELA"
     gene            complement(857..2092)
                     /locus_tag="PKV52_01390"
     CDS             complement(857..2092)
                     /locus_tag="PKV52_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD98901.1"
                     /translation="MFNAHLKMAFASLRSAKIRSFLTMFGVVIGVSSVLIAVSVGQGV
                     KQQVLSQITQLGNDVVTVIPGKSFSTDSKGNITGFNASAIQGVSTLRNEDIEAIKKID
                     GITSVSPGVYISGVATTPERADYSGATIIATTPDIKDVLASKLEYGEFFTENDAQKKV
                     VVLGSNVANDIFNQRDPIGRLVTIRGEDFVVRGVLALAPESPINVGFNFNDTIYMPMK
                     TGEKLVGASGTPQINQITIKLANEKNATEISNAIKETLLKQHKGQEDFTVIQQEDFLQ
                     ATGQVFGLLTAFVAAVAGISLFVGGVGIMNIMLVSVSERTREIGIRKAVGATNRQVVG
                     QFLVEAVVLSVIGGIIGIAASFVAAYFIRLYTPIEPAISLPIIALAFGVSVMVGVVFG
                     IAPAIKAATKDPIQALRQL"
     gene            complement(2095..2853)
                     /locus_tag="PKV52_01395"
     CDS             complement(2095..2853)
                     /locus_tag="PKV52_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD98902.1"
                     /translation="MAIIDLKQVSKVYGFGDATTIAVDATDLKIEKGDFVAIMGPSGS
                     GKSSLLHIIGLLDRPSTGQYKLDNRNVSRFRSGKRAKTRRDKIGFVFQNFNLLSNMTA
                     LENVSLPLAYKGKSTLRRLNQSSVMLAKVGLQSREYYYPHQLSGGQVQRVAIARALIN
                     NPAIIIADEPTGNLDSASSKVIMELLKDIHKGGNTILMVTHNPEITVYANRVLYMLDG
                     KIVIDQPLKKGEKANLENLQKVQQKEDNHKKKDA"
     gene            3136..3223
                     /locus_tag="PKV52_01400"
     tRNA            3136..3223
                     /locus_tag="PKV52_01400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3170..3172,aa:Ser,seq:gga)
     gene            3276..3947
                     /locus_tag="PKV52_01405"
     CDS             3276..3947
                     /locus_tag="PKV52_01405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPD98903.1"
                     /translation="MTILSVYAHHEATSLTSALKNVTISVLSHQGHTVLESDLYGSGF
                     AAKAEKYDFTTTSGQHFNYMLEQKHAQLEGMSFAPDIVAEIEKLAQADLVIFHTPLWW
                     FSVPAVLKGWFDRVLAMGVAWDGGKIYENGLLRGKRAMLCVTAGGPNDYYRIDGKHKA
                     TIPQILHPIQHGTLAFCGFDVLEPYIVLNALGKNHQQLQSVLTEYQFKIEHLVDSPTY
                     FHKFD"
     gene            3976..4671
                     /locus_tag="PKV52_01410"
     CDS             3976..4671
                     /locus_tag="PKV52_01410"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HPD98904.1"
                     /translation="MKQPHIFRSTYGGVFMALVLFSLFCANLSFIRVSITHSTSLLFL
                     LWNLLLAWVPLIFAWLLYRKTLQSGLTWSKRNIIYFIFWLLFLPNAFYLTTDFVHLKG
                     YFDDPQRIFDIVLLISYAINGIILGMVALLLVHVSAVKRFKNNGHWLPVAVLLMSGFA
                     IYLGRYLRWNSWDVFINPFGLLFDVSDRIVNPSDHLLTFSTTLLFFSFYGFLYLIVWR
                     FYQLASTLQQESK"
     gene            4693..>5092
                     /locus_tag="PKV52_01415"
     CDS             4693..>5092
                     /locus_tag="PKV52_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98905.1"
                     /translation="MKQLTQAIQTSVKHLYGIDITNVDLTRPDTQFGDYATNTAMQLA
                     KQLKQPPRHIADALVADITSKNYPWLTSLSVAGPGFINAFLSDDFIFKNINPVPDKVL
                     ADKVVIAEYSDPNAFKALHAGHLYTTLVGNA"
BASE COUNT         1407 a         1085 c         1074 g         1526 t
ORIGIN      
        1 gtaggtgcaa acacgcttgt cgtggcctct agctattctg gcaatacaga agaaaccctg
       61 tctgcactcg cccaagccga agaaaaagga gctaagatag ctgttatagc aagtggtggt
      121 acgttagcgc atattgccca agaaaaaaac tatccctttt tgcaacttcc gcaagtaagt
      181 cagcccaggt ttgcagcgtt gtatggttta aatgcactcg ttaccctttt ggcaagcggt
      241 ggcatcgtgc cacagacggc agtacaggaa ctgcaaggca acgccgagtg gcttaaacaa
      301 catgtcttgt cgtggagacc agacgtacca acacaggaca actatgctaa acaaatagct
      361 ttagatgcca ttggaacatc gcccgtaata tacagcagca cagaattgtt tccggctgcg
      421 tacaaatgga aaattagctt taacgaaaac gccaaaaatg tagcatggtg caacgcctat
      481 ccagaattta atcataacga atttttaggc tggagcagcc atccgataga caaaccatat
      541 accgttatag atttacggag caacctagat cacccaaggg tgcaaaagcg gtttgcattg
      601 tctgcgaaac tcataagcgg taaacgccca gcgcccattg tcatcgatgt agtaggcgaa
      661 acacatctgc agcagctatt atgggcggta gcgcttggcg attttgtatc actgtatact
      721 ggcttgctaa atggcttaaa ccctacacct gtagatctta tagaaaagtt caaaaaagaa
      781 ctcgcctaag gcatcagtgt tgatccatca agtatcattg ttattacacg gcttatttta
      841 agtgcagaac ttgtaattat agctggcgga gggcttggat tgggtctttt gtggcagctt
      901 taatggctgg ggcaatacca aacaccacac caaccatcac agaaacccca aatgcgaggg
      961 cgataatagg cagtgatata gccggttcta taggggtgta taatcgtata aaatacgctg
     1021 caacaaacga agcagcaatt ccaataatac ctccaataac agacaacaca actgcttcta
     1081 ccaagaactg cccaacgacc tgtctattgg tagcgccaac agcttttctg atgcctattt
     1141 cgcgggttct ttcacttaca ctaactagca taatgttcat aataccaaca ccacccacaa
     1201 acaaagatat accagctacg gcagcaacaa acgcagtcaa cagaccaaat acttggcccg
     1261 tcgcttgcaa aaagtcttct tgctgtatta cggtaaaatc ttcctgacct ttatgctgtt
     1321 taagcaatgt ttctttaata gcattgctaa tttctgtggc atttttttcg tttgctagct
     1381 taattgtaat ttggttaatt tgtggagtgc ctgatgcgcc aaccaacttt tcgcctgttt
     1441 tcataggcat gtaaatggtg tcattaaaat taaaccctac atttattggg ctttctggtg
     1501 caagtgctag gacaccacgt actacaaaat cttcaccacg tatggttact aatcttccta
     1561 ttgggtcacg ttggttaaaa atgtcattgg cgacattgct gccaagcacc acaacttttt
     1621 tttgggcatc gttttctgta aagaattcac cgtactctag tttacttgca agcacatctt
     1681 ttatgtctgg tgtggtggcg ataattgttg caccagagta gtcagcccgt tctggtgtgg
     1741 ttgctacccc agaaatatac actccaggac ttaccgatgt tataccatct atttttttaa
     1801 tcgcttctat atcttcgttt cgtaaagtgc tcaccccttg aatagccgat gcattaaacc
     1861 cagtaatatt gcctttgcta tctgtagaaa acgattttcc tggtattacc gtcacaacgt
     1921 cgttaccaag ttgtgtaatt tggctgagta cctgttgttt aacgccttgc ccaacactca
     1981 cagcaattaa cacgctactc acacctatta ctactccaaa catcgttaaa aagctgcgta
     2041 ttttggctga acgtagacta gcgaatgcca ttttaaggtg ggcgttaaac attgttacgc
     2101 atccttcttt ttgtgattat cttctttttg ttgaactttt tgtaagtttt ctaggttggc
     2161 tttttcgccc tttttaagcg gctgatcaat cactatttta ccgtctagca tgtataacac
     2221 acggttggcg tacaccgtta tttctgggtt atgggtcacc attaatatgg tattaccgcc
     2281 tttatgaatg tcttttagca gttccataat gactttactg cttgcactat caagattacc
     2341 tgttggttca tcggctatga tgatggcagg gttatttatg agagcacgcg caatagcaac
     2401 acgttgaact tgcccaccgc ttaattgatg tgggtaataa tactctctag attgtaaacc
     2461 tactttggca agcatgacag aagactggtt tagtcttcgt agggtacttt tgcctttgta
     2521 ggcaaggggg agtgaaacgt tttctagggc tgtcatgtta gataataggt taaaattttg
     2581 gaacacaaac ccaattttat cgcgccgggt tttggcgcgc ttgccacttc taaagcgcga
     2641 aacattacgg ttatcgagct tgtattgccc tgtagatggc cggtctagta gcccaataat
     2701 gtgcaggagg ctacttttgc cagagccact tggccccata atggctacga aatcaccttt
     2761 ttctatcttt aaatcggtgg catcaacagc aatggttgtg gcgtcgccaa acccatacac
     2821 tttgcttact tgttttaaat caataattgc cataaaccgt actgctcatt ttacctctat
     2881 aaaacaactt tattcaagag aagaatagag tataatttac tatagtacgt tttttagcgc
     2941 ttggaggggt cgcctagtgg cctatggcgc acgcttggaa agcgtgctac agcgcaagcc
     3001 ctcgaggtga gaaatgccag tggcattttg caaggcaatt ttccgatgaa aacttgtttt
     3061 caatagtgaa attgcgggcc cgccgaaggt gggtcgaaat cccagggatt caaacccagt
     3121 acacttaaca atcatggagg ggtcgcctag tggcctatgg cgcacgcttg gaaagcgtgt
     3181 tgggcgcaag ccctcgaggg ttcgaatccc tctccctccg ccatgtacag aagctattca
     3241 ttaaacagtt tgtttgaagg tgctatcata aaagcatgac catattatct gtctatgcgc
     3301 accacgaggc tacgtcactt acgtctgcgc ttaaaaacgt tactattagc gtgctgtcgc
     3361 accaaggaca cacagttcta gaatctgatt tatatggcag cggctttgca gccaaagcag
     3421 aaaaatatga tttcacaaca accagtggcc aacactttaa ctatatgctg gaacaaaaac
     3481 atgcccagct agaaggtatg tcgtttgcgc cagacatcgt ggcagaaata gaaaagcttg
     3541 ctcaagcaga tttagttatt tttcacactc ctttgtggtg gtttagtgta ccggcagtat
     3601 taaaaggctg gtttgaccgt gtgctggcta tgggtgttgc gtgggacggt ggtaaaattt
     3661 acgaaaacgg gcttctaagg ggtaaacgtg caatgctatg cgtgaccgca ggcggaccaa
     3721 acgattatta tcgtatagac ggtaaacaca aggcgaccat accgcagatt ctccatccta
     3781 tccagcatgg tacattggcg ttttgcgggt ttgacgtgct agaaccgtac atcgtactga
     3841 atgcacttgg taaaaatcac cagcaacttc aaagtgtatt aacggagtat cagtttaaga
     3901 tcgaacattt agtagatagt ccaacatact tccataagtt cgactaatat ttataaatat
     3961 tttataatta ctgtaatgaa gcaaccacat atatttcgta gcacgtatgg tggtgtcttt
     4021 atggctttag tgctctttag cttattttgc gcgaatttaa gttttatacg ggttagtatt
     4081 acccactcta caagtttatt gtttttactg tggaacctac ttcttgcatg ggtaccttta
     4141 atctttgcgt ggctgctata ccgtaagact ttacaaagtg gtcttacgtg gtcaaaaaga
     4201 aatattattt actttatttt ttggttacta tttttaccaa acgcatttta cttgaccact
     4261 gatttcgtac acttaaaagg ctattttgat gacccgcagc gtatttttga tatcgtgttg
     4321 cttattagct acgccataaa tgggataatt ttagggatgg ttgcgctgtt actggtgcat
     4381 gttagtgctg taaaaagatt taaaaacaac gggcattggc ttccggtagc cgtgctgcta
     4441 atgagtggat ttgcaatata tttaggtcgt tatttacgat ggaacagctg ggacgtgttt
     4501 attaacccgt ttggtctgct atttgatgtt tcagacagga ttgttaaccc cagtgatcat
     4561 ttgcttactt ttagcacaac gttactattt tttagctttt atggattttt gtatttaata
     4621 gtgtggcgct tctaccagct ggcaagcacc cttcagcaag aaagtaagta atctgttaca
     4681 ataccaatcg ttatgaagca gcttacacaa gccatccaaa ctagtgttaa acatttatat
     4741 ggtatagata ttacaaacgt agatcttacg agacctgata cccaatttgg cgattacgca
     4801 accaatacag cgatgcaact tgccaaacaa cttaaacagc caccaaggca catagccgat
     4861 gcgctggttg ctgatattac ttctaaaaac tatccgtggc tgacgagttt gtctgtggca
     4921 gggcctggtt ttattaatgc ttttttaagt gacgacttta tatttaaaaa tattaatcca
     4981 gtaccagaca aggtactggc agataaggta gttatagccg aatattcaga cccaaacgct
     5041 tttaaggcac ttcatgctgg gcacttgtat actacgctcg tgggtaacgc gg
//
LOCUS       DAOPLS010000037         4902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_18709_length_4902_cov_4.251289, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000037 DAOPLS010000000
VERSION     DAOPLS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18709_length_4902_cov_4.251289"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1462
                     /locus_tag="PKV52_01420"
     CDS             29..1462
                     /locus_tag="PKV52_01420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPD98906.1"
                     /translation="MKSNASFIYALALILGDFIALLAAFIVAYILRVTLDSRSLINQI
                     SSIEYLKTWIMLLPVWLLIFGFLGLYRRNVYDYRWKEMVHLAIGSILGVMTIITYDFI
                     VDEPIFPARLVPAYGLVVGFLLLVLGRTLMRAARMFMWRTGFGVNNVLVIGAGQSLKN
                     FVSGLNHPSRTGYRVVGIATKDELPKKLQHLQIEDYKEALAVVGSTNVYTIVVTGLDD
                     DSRVANEALATAQANHIAYKYIPSQAGMLSNNIDVELFQGLPVVSVHQTALTGWGRIA
                     KRLFDIVASGFGIIILSPVYLLIALVIWLSDFGSPLFKQDRLSRFNTIIHIYKFRTHN
                     KRYSGMSPEEAFTAMGKPQLIKKYRENGDFLKKDPRVTPIGRFLRKTSLDELPQLFNV
                     FKADISLVGPRALVPQELKNYAYKDLILSVKSGITGLAQISGRKSIGFEERRALDLYY
                     VQNWSFWLDIKILFRTVIDVLTGRGAK"
     gene            1464..2582
                     /locus_tag="PKV52_01425"
     CDS             1464..2582
                     /locus_tag="PKV52_01425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD98907.1"
                     /translation="MAKKSTKKIAIVSDWLTSIGGAERVLLAMHELYPDAPIYTSQYD
                     PKKINWFDDADVKTTWLQSLPKKSTVRKLLPVLRRQAFQHIDLTQYDIIISSSGAEAK
                     AVKKLKKGATHITYCHAPTHYYWSRYQEYLKEPGFGMLNPVARAGLKIFGGRMRKWDY
                     KVAQRPDVLIANSNHIKQKIEEYYDRDAIVIHPPVDIDRFSKYAPVTKRKGLIITGRQ
                     VPYKRFDLAIAAAKKLNMPLTVIGNGSMHKKLVAQAAGHKNITFLTNVTDTELPKLLS
                     KAELFVFPGVEDFGIAPVEAMAAGTPVVAYGEGGALDYVTDATGVLFHRQTADALADA
                     IVTALGSKWDHKKIGKEVKKFNNNNFKKQFSAVVKQYL"
     gene            complement(2585..3247)
                     /locus_tag="PKV52_01430"
     CDS             complement(2585..3247)
                     /locus_tag="PKV52_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98908.1"
                     /translation="MKNQKGITAIVVLVVVLIVAVVGLAGYYVYNMQKDKNEPTVAPT
                     NSQQDNTKNTANQNTTLAKEICTKDKATCIKYPDFWEATIDTQEDEFAGPIQIETITI
                     RNEATGSELNYTSDVDGLGGGCMDGDISTNITKVVPSSNYQGASVVTVLSDTDQEEVY
                     LYKDETMTPTVGVSTSCAYAYISLNQNMTNDYVFSLQASSANKADTAVLENMVGSFTS
                     TH"
     gene            3367..>4902
                     /gene="glmS"
                     /locus_tag="PKV52_01435"
     CDS             3367..>4902
                     /gene="glmS"
                     /locus_tag="PKV52_01435"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPD98909.1"
                     /translation="MCGIVGYVGRGNALSPVITGLQNLEYRGYDSAGVAFCTDNTIRS
                     IKQAGRVQALESELAGLKQIKPNTAIGHTRWATHGAPTKNNAHPHSNSSKTIFVVHNG
                     IIENHEEIRAMLQKQGYNFVSHTDTEVVPHLIDYFYKKFERMDKAFEVAIRELKGAYA
                     IVMTTSQEPHKIYAAKLSSPLCIGIGKDANMVGSDASAVADAAQKIIYLEDYELAIIE
                     RKSYRIKNFLKKQPVNRPPEDIVLEDVAASKGHYAHYMLKEIHEAPKTIQNAILGRIK
                     LETNQIKLGGIESVANQLKHIDRIIIIACGTSYYAGLVGEYLLEEIANIPVEVQLASE
                     FKYRKEPLTANTAVLAISQSGETADTIAALKKVESSGVLRLGIVNAPGSTIARITDAG
                     IYCHAGPEISVASTKAFIAQSSVLALLALWLGKGYTSLYKPLLTELAHLPKKAEEVLT
                     LAPHIQKIAKKYAHHRDFMYLGRAYEFPMALEGALKLKEISYIHAEGYAAGEMKHGPL
                     ALID"
BASE COUNT         1475 a         1104 c          987 g         1336 t
ORIGIN      
        1 atacccctaa gaggtagtac caatacatat gaagagcaat gcatcattta tatacgctct
       61 cgccctcatc ttgggtgatt ttatcgctct gcttgcagcg tttattgttg cctatatatt
      121 gcgcgttacg ctagacagtc gttcgcttat taaccaaatt tcatctatag aatatttaaa
      181 aacctggatt atgctactgc ctgtttggct gttaatcttt ggttttttgg ggctgtatag
      241 aagaaacgtc tacgattacc gctggaaaga gatggtgcac ttggctattg gtagcatact
      301 tggtgtaatg acaattatta cctatgattt tattgtagat gagccaatct tccctgcacg
      361 gttagtgcca gcatacgggc ttgtggttgg cttcttatta ttggtgcttg gccgcacact
      421 aatgcgcgct gctcgtatgt ttatgtggcg cactggcttt ggggtaaaca acgttttggt
      481 tattggcgct ggccaaagcc taaagaattt tgtatcaggc cttaaccatc catcgcgcac
      541 cgggtaccgt gttgtaggta tcgctaccaa agacgagcta cccaaaaaat tgcagcacct
      601 acaaatagaa gattacaaag aggcgctcgc tgttgttgga tctaccaatg tttacaccat
      661 tgtagtcaca ggcctagatg atgacagccg cgtagcaaac gaagcgctcg ctactgccca
      721 agccaaccat atcgcttaca agtacattcc atcgcaggca ggcatgctga gtaataatat
      781 tgatgtagag ctgtttcagg gcttgcctgt ggtgtctgta caccaaaccg ctcttactgg
      841 ctgggggcgt atcgctaagc gattatttga tattgttgcc agtggtttcg ggattattat
      901 tttatcaccc gtgtatctcc ttattgccct tgtcatttgg ctaagcgact ttggttcacc
      961 gctctttaag caagaccgct taagccggtt taacaccatc atacatatct ataagtttcg
     1021 cacccataac aagcgctaca gtggcatgag cccagaagaa gccttcactg ccatgggtaa
     1081 accacagctt attaaaaaat accgcgaaaa cggcgacttc cttaaaaaag atcctcgggt
     1141 aacaccaatt ggtagatttt tgcgcaaaac aagcctagac gaactacctc agctttttaa
     1201 tgtttttaaa gcagatatta gcctcgtagg cccccgcgcc ctagtgcccc aagagctcaa
     1261 aaactacgcc tacaaagacc tcattctaag tgtaaaatct ggcataactg gtttggcaca
     1321 aatttcaggc cgtaaaagca ttgggtttga agaacgccgg gcgctagacc tctactacgt
     1381 acaaaactgg agtttttggc tcgatattaa aattttgttt cgcactgtca tagatgtatt
     1441 aacagggaga ggagctaaat aaaatggcaa aaaaaagcac taaaaaaata gcaattgtta
     1501 gtgactggct aacaagcatt ggcggtgccg agcgtgtact acttgctatg cacgaactct
     1561 acccagatgc acctatttat accagccaat acgaccctaa aaaaattaat tggttcgatg
     1621 acgcagatgt taaaacaacg tggctgcaaa gcctgccaaa aaaatctacc gtacgtaaat
     1681 tattacctgt tttgcgccgc caagcttttc agcacataga tctcacgcag tacgatatta
     1741 tcatttcgtc tagcggagca gaggctaaag ctgtcaaaaa actaaaaaag ggtgccaccc
     1801 acattaccta ttgccatgca cctacacact actattggag ccggtaccaa gaatacctta
     1861 aagaacctgg ttttggcatg ctcaacccag tagccagagc aggcttaaaa atattcggtg
     1921 gccgaatgcg taaatgggac tataaagttg cccagcgacc agacgtgctt attgccaaca
     1981 gcaaccatat taaacaaaaa atagaagaat attatgatcg cgacgccatt gttattcacc
     2041 caccagtaga tatagataga tttagcaaat atgcgccagt tacaaaacgt aaaggcctta
     2101 ttattactgg ccggcaagtg ccctacaaac ggtttgacct agccattgcc gcagctaaaa
     2161 agcttaatat gcccttaacc gtaattggca atggctctat gcacaaaaaa ttagtagctc
     2221 aagcagcggg tcataaaaat attacctttt taaccaatgt aaccgataca gagctaccca
     2281 aactactatc taaggccgag ttatttgtgt tcccaggtgt agaagatttt ggtattgcac
     2341 ccgtagaagc tatggcagct ggtacgcccg tagttgccta tggcgaaggt ggtgcactag
     2401 actacgttac cgatgccact ggcgtgctgt ttcatcgtca aacagcagat gcgcttgcag
     2461 atgctattgt tactgcactt ggttctaaat gggatcacaa aaaaattggt aaagaagtca
     2521 aaaagtttaa taacaataac tttaaaaaac aattttcagc agtcgttaaa caatatttat
     2581 agccctagtg ggtgctggta aacgatccaa ccatgttttc tagtactgca gtatctgctt
     2641 tgttggcact tgatgcttgc agtgaaaata cgtaatcgtt ggtcatattt tgatttaagc
     2701 ttatatatgc ataggcacac gacgtactca cacctactgt tggtgtcatg gtttcgtctt
     2761 tgtacaaata cacttcttct tggtcagtat cagataaaac agttactaca ctcgccccct
     2821 ggtaattgct agatggaaca acttttgtaa tattggttga aatatcaccg tccatacaac
     2881 caccaccaag tccatctacg tcactcgtat aatttaattc agaacccgta gcttcgtttc
     2941 ttattgtaat ggtttctatc tgtatagggc cagcaaactc atcttcttgc gtatctatgg
     3001 ttgcttccca aaaatcagga tattttatac atgttgcctt gtcttttgtg caaatttctt
     3061 tggcaagcgt ggtgttttga ttagcagtat tcttggtatt atcttgctga ctatttgtag
     3121 gagcaaccgt gggttcgttt ttgtcttttt gcatgttata tacgtagtag cctgcaagcc
     3181 caactaccgc cacaatcagc accactacaa gcactacgat tgccgttatg cctttttgat
     3241 ttttcatgga tgaattttcc ttctttatat tatattgctt attcttacca acattttatc
     3301 atcaccacct atcaaacccc aaactacgat atactaaata ctatctacta agcggagata
     3361 aaaaggatgt gtggaatagt cggatacgtc gggcgtggca atgcactgtc acctgttatt
     3421 accggtttac aaaacttaga atatcgcggt tacgattcgg ctggcgtagc cttttgcaca
     3481 gacaacacta ttcgcagtat aaagcaggca ggccgtgtac aagcgctaga atcagaactg
     3541 gcagggctta aacaaataaa acccaatact gctattggcc atacccgctg ggcaacgcat
     3601 ggtgcaccta ccaaaaataa tgcacatccg catagcaatt catctaaaac aatatttgta
     3661 gtgcataacg gcatcataga aaatcacgaa gaaatacggg ctatgttaca aaaacaaggc
     3721 tacaattttg tatcacacac agatacagaa gtcgttccac acttaattga ctacttttat
     3781 aaaaaatttg aacgaatgga taaagctttc gaggtagcta tacgcgagct aaaaggtgcc
     3841 tacgctattg ttatgaccac tagccaagaa ccacataaaa tatatgctgc aaaactatct
     3901 agcccactgt gtattggcat aggtaaagat gccaacatgg ttgggtctga tgcttctgcg
     3961 gtcgccgatg ctgcccaaaa aataatttat ctagaagatt acgaactcgc cattatcgaa
     4021 cgcaaaagct atcgtattaa aaactttctc aaaaaacagc ctgtaaaccg cccgccagaa
     4081 gatatcgtgt tagaagacgt tgctgccagt aaaggccact acgctcatta tatgctcaaa
     4141 gaaattcacg aagctcccaa aacgattcaa aacgctattc tagggcgcat taaattagaa
     4201 acaaaccaaa taaagctggg tggtatagaa tctgtcgcta accagctaaa acacatagac
     4261 cgaataatca ttattgcctg tggcacatct tactacgcag gtttagtagg cgaatattta
     4321 ctagaagaaa ttgctaatat tccggtagag gtacaactag ccagcgaatt taaataccgc
     4381 aaagagccgt taactgctaa tactgcagta ctagccatat cacaaagcgg cgaaacagca
     4441 gataccattg cagcgcttaa aaaagtagaa tctagcggtg tgctaaggct tggtattgtg
     4501 aacgcccccg gcagcaccat cgcacgtatt accgatgctg gcatttattg ccatgctggg
     4561 ccagaaatat ccgttgccag caccaaggca tttattgctc aaagttcggt actagcgctc
     4621 ttggcgctat ggctgggcaa aggctacaca agcctctata aaccgctcct aacagagctt
     4681 gcccatctgc caaaaaaggc agaagaagta ctaacattag caccacacat ccaaaaaatt
     4741 gccaaaaaat atgcacatca cagagatttt atgtaccttg gccgtgccta cgagttcccc
     4801 atggctctag aaggcgcatt aaagctcaaa gaaattagct acattcatgc agaaggctac
     4861 gctgcaggcg aaatgaaaca cggcccacta gcgctcatag ac
//
LOCUS       DAOPLS010000038         4848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_18993_length_4848_cov_3.693720, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000038 DAOPLS010000000
VERSION     DAOPLS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18993_length_4848_cov_3.693720"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PKV52_01440"
     CDS             complement(<1..1130)
                     /locus_tag="PKV52_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPD98910.1"
                     /translation="MNQQLHKHRQELLTNIPKDATAILVAGNAPLSRNADTTYPFRQD
                     SNILYLSGITLPNIALYIDTKTGNSYLVLPRHTKVEIMFDDVHEYEYAATAAGLNGCI
                     TQTELLVRLQKLSKKGEVLFNKPARARSHGQYANPYRRYWLNRLTKAGIVPIDVRPYV
                     AAMRMIKKPHEIAAITSAVQVTQTVLSKYINNPAELVGRTETSVANEITAAFYENNMM
                     NAYQPIVATDQNAVIVHHAPSDTPIKTGAGLLFDVGAEHCGYAADISRTIVVGHNQKL
                     QMLIDDVAAVQANIIASLKPGIFWKDIQLQAQTQLLDVAKKHALITQEPVQTVFPYAI
                     GHHVGLDVHDAADYSLSLSEGMVITIEPGLHSKKHGIGIRIE"
     gene            complement(1134..2630)
                     /locus_tag="PKV52_01445"
     CDS             complement(1134..2630)
                     /locus_tag="PKV52_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPD98911.1"
                     /translation="MNKALEKKLETIPSDPGVYFHKNSNGEIIYVGKAAVLKNRIRQY
                     FHKSRVRDAKTDALVSEIADTDWIIVADEVEALFLEAEMIRRYQPQYNILLRDDKSVA
                     YVRIDSKSRAPSVTITRRPLDDKADYYGPFLQTLPLRRSLKYLRKIFPYSTHHTLPNR
                     GCLQYHLGLCPGPETENYSEKDYKDNLKKLSLYLKGQRVALITQLEQEMNLAADNKAY
                     EEAAKKRNTITALKSLRARIIFSDRENLDLSNDHALFDFATLLGLATPPKRIEGYDIS
                     HMSGTDTVASMVVFTNGIADKGAYRKFKMRIPGNDDFAHMEEVIARRLKKSSQKTWQL
                     PDIFLIDGGKGQLAAALKARNAAGLTIPMIGLAKKYEEIVLHTKKSYVSVNLEKLHQL
                     QGYITDTSDDFQVLDLPNTAHLIKLLQRIRDESHRFAVSYHSTLKISRQRESQLDTIP
                     GIGPITKRTLLKKFGSLAGIANATLDEIASAVGSKKAHLIMSYIKKEE"
     gene            complement(2641..3531)
                     /locus_tag="PKV52_01450"
     CDS             complement(2641..3531)
                     /locus_tag="PKV52_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HPD98912.1"
                     /translation="MEKVILYYKFVPVADPETTKLWQRELCERYGLKGRIIISTHGIN
                     GTLGGPIDGLKAYVKAMNVSIFKKTVYKWSEGSADDFPRLSIKVRPELVAFGAPDAVK
                     VDNSGVIGGGKHLKPEEVNALVAERGDDVIFFDGRNAYEAQIGKFKNTVVPNVRYTRD
                     FLKELDDPKYNDIKDKPIVSYCTGGIRCELLSVHMKNKGFKEVYQIDGGIVKYGETYK
                     DDGLWEGKLYVFDNRMVTGFSKDAKDIGECVHCQGKTSNFENCANVACNDLILICKDC
                     HTTTQTCAKSSCKQTVATKA"
     gene            3668..>4848
                     /locus_tag="PKV52_01455"
     CDS             3668..>4848
                     /locus_tag="PKV52_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD98913.1"
                     /translation="MTKTANSSLSALKTMRIYLTYMKKYPLMLATIIILLPITVISAN
                     VLLPIIYAEAINTVSSLLGSGESFMPLFGTLLVQSIILIGISWTAWRIIGFVISTFEY
                     RVKRDLEQAIFKHLTTHSYDFHVNSFSGSLVAQTNRMTNAFERLFDSFYFDLYVLVIK
                     VTAIMVVLLPKSPTAAMIIIVWISLYIIMMSILQIMKMPYSRATAAADSNVTAALADS
                     LANIFTIITFGKKRSEQKKFNAVSQKRYRIALKDWYISEAIFAFQGIMMFTIEAVLIW
                     VTIDLALQGKIGIGEIVLVQIYIMSIIGSMWNFGRVIRNIERSLSDAYEMTLILGKDH
                     AIKDPRHPKPVAMTKGSIRFNKVVFSYEEKQPLFTDFNVSMKAGQKVGLVGPSGGGKS
                     T"
BASE COUNT         1320 a          957 c          954 g         1617 t
ORIGIN      
        1 tcttctatac ggatgccaat gccatgcttc ttgctgtgaa gccctggttc tattgttatg
       61 accatacctt cagataaaga cagcgaataa tctgctgcgt catgaacatc gagccctacg
      121 tgatgaccaa tggcataagg aaacacagtc tgcacgggtt cttgtgtgat aagtgcatgt
      181 ttttttgcta cgtctagtaa ttgggtttgg gcttgcaatt gtatgtcttt ccaaaatatg
      241 ccaggtttta aagaagcgat tatgtttgcc tgcacagcag ctacatcatc tatcagcatt
      301 tgaagctttt ggttgtgacc gacgacgatt gttcttgaaa tatctgctgc atacccacag
      361 tgttctgcac caacgtcaaa tagcagccct gcgcctgttt tgattggtgt gtctgatggt
      421 gcgtgatgca ctatcacggc attttggtca gttgctacaa ttggctgata ggcgttcatc
      481 atattatttt cataaaaagc tgcggtaatt tcgtttgcaa cagatgtctc tgtgcgacct
      541 acaagttctg ctggattgtt aatatattta gataaaacag tttgcgttac ctgtacggct
      601 gaagttatcg ctgcaatttc atgtggcttt tttatcatac gcatggctgc cacatacgga
      661 cgaacatcta taggcacaat acctgccttt gtaagcctat tgagccaata acgacgatac
      721 gggttagcgt attgtccatg gctacgtgca cgtgctggct tattaaataa gacctcgcct
      781 ttttttgata gtttttgtaa acgaacgagc aactctgttt gagttataca gccgtttaga
      841 ccagcggcag tcgcagcata ttcgtattca tgaacatcat caaacattat ttctacttta
      901 gtatggcgtg gtaacacaag atatgaatta cctgtttttg tatcgatata gagcgcaata
      961 ttaggcaaag taatacctga taaatagagt atgttactat cttgcctaaa tggatacgtt
     1021 gtatctgcat tgcggctcaa cggagcattg ccagctacca aaatagcagt agcatctttg
     1081 ggtatattgg taagtagttc ttgtcggtgc ttatggagct gttgattcat taattactct
     1141 tctttcttta tgtaagacat tattaggtga gcctttttgc tccctacagc gctagctatt
     1201 tcatctagtg ttgcattggc aatacctgca agtgatccaa atttttttaa taacgtacgc
     1261 tttgttatgg gcccaatccc tggaatagtg tctagctgac tttctctttg acgagatatt
     1321 tttagtgtac tatggtagct cactgcaaat ctatgtgatt catcacgaat gcgctgcaac
     1381 agttttataa gatgtgcggt attgggaaga tctaaaacct gaaaatcatc tgaagtgtct
     1441 gtaatatatc cttgcagctg atgtaacttt tcaaggttca cgcttacata actttttttt
     1501 gtatgaagta ctatctcttc atattttttg gcgagtccta tcattggtat ggttagacct
     1561 gctgcatttc gggcttttag agctgcagcc agctgacctt tcccaccgtc tattaaaaaa
     1621 atatccggta gttgccacgt tttttgtgaa gattttttaa gacgtcttgc aataacttct
     1681 tccatatgag caaaatcatc gttacctggt atacgcattt taaattttct atacgctcct
     1741 ttgtcagcaa taccattagt aaaaacgacc atactcgcaa cagtgtctgt accactcatg
     1801 tgactaatgt catacccttc tatacgtttt ggtggtgttg ctaaaccaag gagcgtggca
     1861 aagtcaaata atgcatgatc attagataaa tctaggtttt cgcgatcact aaatataatg
     1921 cgtgctctta gtgactttaa agccgttatc gtattgcgtt tttttgctgc ctcttcatat
     1981 gctttattgt ctgccgccaa gttcatttct tgttctagct gcgttatcag tgccacgcgc
     2041 tgacctttta aatagaggct tagttttttg agattgtctt tgtagtcttt ttcgctgtag
     2101 ttttcggttt ctggaccagg gcataaaccc aaatgatatt gcaaacaccc acgatttggt
     2161 agcgtgtggt gcgtagagta cggaaatatt ttacgtaggt actttaggct tcttctaagt
     2221 ggcagggttt gtaaaaatgg gccatagtag tcagccttgt catctaatgg gcgccttgta
     2281 atggtgactg acggggcgcg tgatttactg tctatgcgta cgtaagcaac cgacttgtca
     2341 tcacgtagta agatattata ctgtggttga tatcgccgaa tcatttcggc ttctaaaaat
     2401 aatgcttcaa cttcatctgc aacgataatc caatcggtat cagcaatttc tgaaacaagt
     2461 gcgtccgtct tagcatcacg gacccgactt ttgtgaaagt actgtcgaat acggtttttt
     2521 aagactgcag cttttcctac ataaataatt tcgccattgc tatttttatg aaaatacaca
     2581 cctggatcgc tgggtatagt ttctaatttc ttttctaggg ctttgttcat taccaaaacc
     2641 ttaggctttg gtagctactg tttgtttgca gctagattta gcacatgtct gggtggtagt
     2701 atggcagtct ttgcaaatta aaattaaatc gttacaagca acatttgcac agttttcaaa
     2761 attactggtt tttccttggc agtgtacaca ttcgccgatg tctttcgcgt ctttactaaa
     2821 gcccgttacc attcgattat caaacacgta caatttacct tcccatagac catcatcttt
     2881 ataggtttcg ccgtatttaa cgattcctcc gtctatttga tacacttcct tgaagccttt
     2941 gttcttcata tgaacactga gcaactcaca ccgaatacca ccggtgcagt acgaaacaat
     3001 tggtttgtct ttaatatcgt tatatttagg atcgtctagt tcttttagaa aatctctggt
     3061 gtagcgaaca ttcggaacga ctgtattctt aaatttacca atttgcgcct catacgcatt
     3121 gcgaccatca aaaaatataa catcatcacc acgctctgca actaaagcat ttacttcttc
     3181 tggtttaaga tgcttgccac cgcctataac accactatta tctactttca cggcatctgg
     3241 tgcaccaaaa gcaacgagtt ctgggcgcac tttaatagat aatctaggaa aatcatcagc
     3301 actaccctcg ctccacttat aaacagtttt tttaaatata cttacattca tagcttttac
     3361 gtacgctttt aagccatcta ttgggccacc aagcgtgccg ttaataccgt gtgtactgat
     3421 gataatgcga ccttttaaac cataccgttc acagagttct ctttgccata gtttcgtcgt
     3481 ctctgggtca gctaccggca caaacttata gtataaaatt actttttcca ttgcacctat
     3541 tataccagat atggtacact tacgaacaaa tgacgatgga taatttgtta tccctctcgt
     3601 gagtaacgct cttcgatgtc aattgtgacc gctcgactaa aacaataaaa gtaatcagga
     3661 cattagtatg accaaaacag ccaatagctc gctgagtgcg cttaaaacaa tgcgcattta
     3721 cctaacatat atgaaaaagt atccactcat gttggctact attattatcc ttttacccat
     3781 aacagttatc agtgcaaatg tgttattgcc tattatttac gcagaagcta ttaatacggt
     3841 aagtagtttg cttggtagtg gtgaatcgtt catgccacta tttggtacgt tgctggtaca
     3901 atcaattatt cttataggta tttcgtggac tgcatggcgt attattggct ttgttataag
     3961 tacgttcgaa tatcgggtaa aaagagattt agagcaagcg atatttaaac accttacgac
     4021 tcatagctac gattttcatg ttaatagttt tagtgggtct ttggtggctc aaacaaaccg
     4081 catgaccaat gcgtttgagc gattgtttga ttcattttat tttgatttgt atgtgcttgt
     4141 tattaaggta acggcaatta tggtggtgtt actaccaaag tcacccacgg ctgcaatgat
     4201 tattatcgtg tggattagct tatacataat tatgatgagt attttacaaa taatgaaaat
     4261 gccatactct cgtgcaactg ctgcagcgga tagcaatgtt actgctgctt tagcggattc
     4321 gcttgcaaat atttttacga taattacgtt tggcaaaaag agatcagaac aaaaaaagtt
     4381 caatgccgtt agccaaaaaa ggtaccgtat tgccttaaaa gattggtata tatctgaagc
     4441 aatatttgcc tttcaaggta tcatgatgtt taccattgaa gcagtactta tatgggtcac
     4501 gatagactta gcattgcaag gaaaaattgg tatcggcgaa attgttttag ttcaaatata
     4561 catcatgagt attattggga gtatgtggaa ctttggtaga gttatacgta atatagaacg
     4621 tagcttgtct gatgcctatg aaatgacgct aatactaggt aaagatcacg ccattaaaga
     4681 cccccgtcat ccgaagcccg ttgcgatgac taaagggagt attcgattta ataaggttgt
     4741 gtttagttat gaagaaaaac aaccgttatt taccgatttt aatgtatcta tgaaagccgg
     4801 ccagaaagta ggattagtag gccctagtgg aggtggcaaa tctaccat
//
LOCUS       DAOPLS010000039         4751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_19535_length_4751_cov_3.231261, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000039 DAOPLS010000000
VERSION     DAOPLS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19535_length_4751_cov_3.231261"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..464
                     /locus_tag="PKV52_01460"
     CDS             51..464
                     /locus_tag="PKV52_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98914.1"
                     /translation="MLRGINYIENANADSITAYAYFAPDDVYTSKIRYYLNGANNALL
                     ADVTPMTADYPIGTLLTGQTKTVTVVNKFYKISGNPTFLYYDANYNQLTSPVTDVQSI
                     KNITTQLYIKKYDSNANDYVSTSVSVSLRNRKYNL"
     gene            461..2029
                     /locus_tag="PKV52_01465"
     CDS             461..2029
                     /locus_tag="PKV52_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98915.1"
                     /translation="MKMNDGHVLTWVLGFMAAIGVILVSTASVISLVLSDAVSTNKNQ
                     LTLNIADAGVNYYLWHLSHAAADFKDGNPSATINTTGDFTGFYGPFVHSYKDDNNKEV
                     GTYTLYIKPKSVGSSVAIVRSIGSTLDGKFRRTIEAEIGAPSFASYSLAGDVALWFGN
                     TENANGPVHSNVGVRMDGGNTDDVTSANATYVPPSNLGGNGYTTRPGVWCNTAVTSPV
                     NCNTRSKADWRYPVPSIDFNAITGDLCNIKKTAFLSFANTAALASSPTACSNVSAPRT
                     DAYIPRVASSFNERRGYLIELNSNTTYNLYSISNETYSYSNNSSYTSSYTSALSRTLI
                     ANNIAIPQSGVIFVEDNVWVRSNPVFNGRVTIASGRLASASEQTSVVIADSLKYDQKD
                     GSDAIGIIAEEDVTIAPYAPPKPGDSTANYPFEVNGALIAKDGHVWFPADYLGRDTPD
                     WRGNSSKKLVYYGSIATRQSWTWLWTSGGTPIDGFEYNETNFDYNLLYAPPPSFPITS
                     TYTILKWREVLVTP"
     gene            2081..3154
                     /gene="pilM"
                     /locus_tag="PKV52_01470"
     CDS             2081..3154
                     /gene="pilM"
                     /locus_tag="PKV52_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPD98916.1"
                     /translation="MTQSILYKDKPLFGLDVGTSSIKVMQLEQIKSNQKTRRVQGYGS
                     IQYDKAAIADGEIKEPDLVAKAIKELFESSLIGDITTRRVAMSLPVARTYNRVLSLPL
                     LKTKELDEAVRLEAEQYIPVPIEELYIDYDVISSNDKGLEVLLSAAPKKIVDSYATLT
                     ELLGLEIVALETSISASGRLVGNAENNDVPTILVDCGSLSVDITIYDKQLIVTGTIPG
                     GGDTFTELIAKKLDVSNQIAHTIKTKYGIAFSKKQDEIIQAIEPILHSMIKEIKKMVR
                     YYEERDGSKKKIGQVITLGGGANLPGLSEYLTDKLRLPARMCNPWTNLDFHKLQPPNE
                     VEKTMYITAAGLSLINSKDIWHD"
     gene            3147..3749
                     /locus_tag="PKV52_01475"
     CDS             3147..3749
                     /locus_tag="PKV52_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98917.1"
                     /translation="MINLLPADVKQDIGYARKNKVLFSWIIILLILLLAIVITIGFGL
                     FFITQRANNLNRLATISEQRVVDQNLAEYQQKAEVFSNDLDIAVKLLENQLLFSKIIR
                     TIGSVLPPGVTLGTLDYSAEDEVLTLNVNGADQRLITTAFENISSSQEKTDNLFTKAD
                     LLKIDCNSLEGGCTGSIVVLLNKNSEFYLINNALKDKGQN"
     gene            3749..4342
                     /locus_tag="PKV52_01480"
     CDS             3749..4342
                     /locus_tag="PKV52_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98918.1"
                     /translation="MNPKKMYYLLVAALVVTLLAIGGVLYLGRGVMLAASEKVIDAKL
                     NIIKAEKTEETYLKNKQIYLANSATAEKLNYLVPTDKEQAAAVETIYSFANNANLTIT
                     SVVFPGSSLDPTVKQKTKVDISQATPVKDLKGVYEIPIEVSISGGGKNSISTNQILNL
                     IESIEASPRNMRITAITYDSTSGEIRLNISLFVKKPS"
     gene            4339..4635
                     /locus_tag="PKV52_01485"
     CDS             4339..4635
                     /locus_tag="PKV52_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98919.1"
                     /translation="MKNITAKFTDSLQQIVHFIVRYRVTLFIVISASILGVVLLGITA
                     MSDAEPSESQIADAEKSVKIIKFNEASVKVFDSLKANNVTIETLFDPNRYDPFN"
BASE COUNT         1597 a          921 c          903 g         1330 t
ORIGIN      
        1 gatggtcttg cgcgtagtaa acttgctgaa agttctttta gggtacaaaa gtgctccgag
       61 gtatcaatta tatagaaaat gctaacgcag atagtattac cgcttacgct tattttgcac
      121 cagacgatgt gtatacatct aaaattcgct actatctcaa tggtgcgaat aacgccctat
      181 tagcagatgt cacacccatg acagccgatt atccaattgg cacgctatta accggccaaa
      241 caaagacagt cactgtagta aataaatttt acaaaatttc tggaaatcca acttttttgt
      301 attacgacgc taattacaac caattaacca gccctgtcac tgatgtacaa tctataaaaa
      361 acattaccac acaactatat attaaaaaat atgactcaaa cgctaatgat tatgtatcca
      421 caagtgtttc tgttagttta aggaatagaa agtacaacct atgaaaatga acgacggcca
      481 tgtactaacg tgggtactcg gttttatggc agctattgga gttatcttgg tgtcgactgc
      541 gagtgtcata tcgctggtat tatctgacgc tgttagtaca aataagaatc agctaacact
      601 caatatcgcc gatgcaggtg tcaattatta cttatggcat ttaagtcatg ctgcggctga
      661 ttttaaagat ggaaacccaa gtgctaccat taacacgact ggcgatttta ctggttttta
      721 tggacctttt gtacacagct ataaagatga caataataaa gaagtaggta cgtatacttt
      781 atatataaag cctaagtcag tcggctctag cgttgcaata gtcagatcta ttggtagtac
      841 attagacggc aaatttagac gaaccataga agctgaaatt ggagccccat catttgcaag
      901 ttatagttta gctggtgatg tagctttgtg gtttggtaat accgaaaacg ccaacggccc
      961 agtacattct aatgtaggtg tccgaatgga cggcggaaat acagatgatg taacaagtgc
     1021 taacgccacg tatgtacccc cttctaactt aggtggtaac ggatacacaa ctcgtcctgg
     1081 tgtttggtgt aatacagcgg taacgagccc agtaaattgt aacactcgtt ctaaggctga
     1141 ttggcgctac cctgtaccat ctattgattt taacgctata accggagatc tttgtaatat
     1201 taaaaaaacc gcattcctat cgtttgctaa taccgcagcg ttagcctcta gtcctaccgc
     1261 atgttctaac gtgagcgcgc caaggacaga tgcatatata ccccgtgtgg cttcatcatt
     1321 taatgaacgg cgaggctatc ttattgagct caatagtaat acgacctaca acttgtatag
     1381 cattagtaat gaaacataca gttattccaa taactcaagc tatacgtctt catataccag
     1441 tgctctaagt cgcacattaa ttgctaataa tattgccata ccacagagtg gcgtcatttt
     1501 tgttgaggat aacgtatggg tacgttcaaa ccctgttttt aatggccggg taaccatagc
     1561 atccggccgt ctggccagcg catcagaaca aacttctgtc gttattgctg attcattaaa
     1621 atacgatcaa aaagatggaa gcgatgcaat aggcatcatc gcagaagaag atgtcacaat
     1681 tgcaccgtat gcgccgccaa agcctggtga cagcactgcg aactatccat ttgaggtaaa
     1741 cggcgctctt atagcaaaag atggtcatgt ttggttccct gcagattatt taggcagaga
     1801 taccccagac tggcgaggta atagtagtaa aaaacttgtc tattacggct ctatagcaac
     1861 tagacaatca tggacatggc tgtggacaag tggcggcact ccaatcgacg gttttgagta
     1921 taacgaaaca aattttgatt acaacctctt gtatgcacca ccaccgagtt ttcctataac
     1981 gagcacctat acaatcctaa aatggagaga agtccttgtt actccataaa tacaccgctt
     2041 atggtatact attggtacat aaagtgggca aaataaaagc atgacacaaa gcatcctata
     2101 caaagataag ccactgtttg gccttgacgt gggtacaagt agtattaaag tcatgcaact
     2161 tgagcaaata aaaagtaacc aaaagaccag aagagtgcaa gggtatggat ctatacaata
     2221 cgataaagca gctattgctg atggtgaaat caaagagcca gatttggtag ccaaagctat
     2281 taaagaacta tttgaatcat cccttattgg tgacataacg acgcgacgag ttgctatgtc
     2341 attacctgtt gctaggacat acaaccgtgt tttatcgctt cctctattaa aaacgaaaga
     2401 gctagacgaa gcagtacgct tagaagctga acaatacata cccgtaccaa tagaggagtt
     2461 atatattgac tatgatgtta tttctagtaa cgataaaggt cttgaagtat tattatctgc
     2521 agcaccaaaa aaaattgttg attcttatgc tacactcacc gaattgctag gattagaaat
     2581 tgtcgcacta gaaacaagta tatctgcgtc tggtcgtttg gttggaaatg ccgaaaataa
     2641 tgatgtccca acaatcctgg ttgattgcgg atcattatct gtggatatta caatctacga
     2701 caaacagctt atcgttacgg gtaccattcc gggtggtggt gatacattca cagagctcat
     2761 cgctaaaaag ttagacgtca gcaatcaaat agcacatacc attaaaacga aatatggcat
     2821 agcatttagc aaaaaacaag atgaaataat acaagcaatt gagccaatac ttcattcgat
     2881 gatcaaagaa attaagaaaa tggtcagata ctacgaagaa cgagatggca gtaagaaaaa
     2941 aattggacaa gttattacac tcggtggcgg tgcaaacttg ccagggctaa gcgaatatct
     3001 aacagataaa ttacgactac ctgcacgaat gtgtaatcct tggacaaatc ttgacttcca
     3061 taaactacaa ccacctaacg aagtagaaaa aactatgtat atcaccgccg caggattatc
     3121 gctcattaat agcaaggata tttggcatga ttaatttact cccagctgat gtgaagcaag
     3181 atatcggtta tgcccgtaaa aataaagtgt tattttcatg gattattata ttacttatct
     3241 tgttactcgc tatcgtcatc acaataggat ttggtctatt ttttataacc caaagagcaa
     3301 acaacttaaa cagattggct acaatttctg aacaacgagt agtagatcaa aaccttgcag
     3361 agtaccaaca aaaagcagag gttttctcta atgacttaga catagcagta aagttgttag
     3421 aaaaccaact gctgttttct aagattatac gtacaattgg gtctgttttg ccacccggtg
     3481 tcacgcttgg aacgctagac tactctgccg aagatgaagt gctgacatta aacgtcaatg
     3541 gcgcagatca gagactcatt actactgcat ttgaaaacat ttctagtagt caagaaaaga
     3601 ctgataactt atttacaaaa gccgatttgt taaaaattga ctgtaattca cttgaaggtg
     3661 gctgtactgg tagtattgtt gtgctattaa ataaaaattc tgaattttat ttaataaata
     3721 atgctttaaa agacaaaggc caaaactaat gaacccaaag aaaatgtatt acctacttgt
     3781 agcagcactt gttgtgacgt tgctcgcaat tggtggcgtg ctatatcttg gtagaggtgt
     3841 aatgcttgct gcatcagaaa aagtcataga tgctaaatta aatatcatta aagcagaaaa
     3901 aacagaagaa acgtatctta aaaataaaca gatataccta gctaattcag ctacagcaga
     3961 gaaattaaac taccttgtac ctacagataa agagcaagct gcagctgtag aaactattta
     4021 tagttttgcg aacaatgcaa atcttacgat tacatctgtt gtttttccag gttctagcct
     4081 agaccctacg gtaaagcaaa aaactaaagt agacatttct caggcaacgc ccgtaaaaga
     4141 cttaaaaggg gtatatgaaa ttcctataga agtaagtatc agcggcgggg gtaagaactc
     4201 tatctcgaca aatcaaatac tcaacctcat agaatcaatt gaagctagcc ccagaaatat
     4261 gcgaattact gctattacct acgattctac tagcggagaa atccgattaa atatatcatt
     4321 attcgtaaag aagccatcat gaaaaatata acagcaaaat tcaccgacag tcttcagcaa
     4381 attgtacatt ttatcgtaag gtacagggta acattattta ttgttatatc tgcatctata
     4441 cttggtgttg tactcttagg cattaccgct atgtcagacg ccgaaccatc tgaaagccaa
     4501 atagccgatg cagaaaaatc agttaagatt attaagttca atgaagcttc tgtaaaagtc
     4561 tttgattccc ttaaggcaaa taatgtaaca atagaaacac tatttgaccc caacagatac
     4621 gatccgttta actagttaac tacctaaaat accactacgt atttatattg atattatttc
     4681 tataggaacc tgcttggcca gtagcttgta taaaatcgtc ttttgcatca agtataattc
     4741 tcgcaaccct g
//
LOCUS       DAOPLS010000040         4712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_19768_length_4712_cov_4.058621, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000040 DAOPLS010000000
VERSION     DAOPLS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19768_length_4712_cov_4.058621"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKV52_01490"
     CDS             complement(<1..560)
                     /locus_tag="PKV52_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPD98920.1"
                     /translation="MAKASAPTKLATSKRTFFTKSEDIALPNLISHQNDSFAWFIKEG
                     LGELFAEISPIDDYTGTKLSLSFKSYYFGNPKMSEAEARENNVSYEAPLMANAELVNK
                     VTGEVKEQEIYLGDYPWMTDRGTFVINGAERVVVSQLIRSPGVFFTADQHGSTNTYGA
                     KVIPNRGAWLEFETAVNGAIYVKIDRR"
     gene            complement(758..1348)
                     /locus_tag="PKV52_01495"
     CDS             complement(758..1348)
                     /locus_tag="PKV52_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPD98921.1"
                     /translation="MHSMLIPTVIESDGRVERAYDIYSRLLKERIIFLGEDVNPHTAN
                     LVVAQMLFLAAEDAKKDIIFYINSPGGSVYDGMAIYDTMQYVKNDVQTVGIGLQASMG
                     AFLLSSGTKGKRMVLPHSKVMIHQPSSGTRGKVTDMEIDLKESLKVKHLLNEILAKNT
                     GQPVKRIQEDAERDYWMSAEEAKKYGLVDKIITTLK"
     gene            complement(1397..1771)
                     /locus_tag="PKV52_01500"
     CDS             complement(1397..1771)
                     /locus_tag="PKV52_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98922.1"
                     /translation="MSPERVISYDGDAEPYVIFEKFIRAHSPLSEEAIDAYLNEARKL
                     LEAGSTFFCRRNFYPTNGDVFDLYSKGKLIGFIAGDDDYSVDVFIDSEDGPQKIGETI
                     ESGSYAENMLEGIARLKSQLAE"
     gene            complement(1811..3100)
                     /gene="tig"
                     /locus_tag="PKV52_01505"
     CDS             complement(1811..3100)
                     /gene="tig"
                     /locus_tag="PKV52_01505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPD98923.1"
                     /translation="MKIIRTNPSDTTIVLTISGTATELAAAKNIAVAKLAPQVKMQGF
                     RPGHAPAELVEKQLNPNTLQEETLNEVLNALYETALRQENIRPVANPKIELKKFVPYT
                     DIEFTAEIEVIGTIKLGNYKKLKAKKQTTDATAKDVEEVLNRLQTQMAEYAEVARAAK
                     TGDRVWIDFDGTDAKGAPVNGAKGQDYPLALGSNTFIPGFEDNVLGMKKDDQKNFTIP
                     FPKDYGVKALQGKKVTFKVTAKKIEETILPPIDDEFAKKVGPVSTVKELKADIKKQLK
                     QERDHQADREYQDALIKELVAASDVPLPESMLTEQIEIVDKEFKQNLVYRGQTFKEYL
                     EATGLDEATYKEKELKPAAQERLKAGLVLSELADIEKVTITPEELEIRLQVLKGQYES
                     DQKMQSELEKPENRREVAARLLTEKTIAKLVSFQNSK"
     gene            complement(3133..4455)
                     /gene="hisS"
                     /locus_tag="PKV52_01510"
     CDS             complement(3133..4455)
                     /gene="hisS"
                     /locus_tag="PKV52_01510"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPD98924.1"
                     /translation="MALSTTPYKGTRDFYPKDFRIRQYIFDTWAETCKRFGYEQYDAP
                     VLEPLELYQAKTSEEIVGEQTYAFTDRGGRAVTLRPEMTPTVSRMVAAQRQELAYPLR
                     WFSIPNVWRYERPQRGRLREHWQLNVDLFGIQDDSADTEVIMLADAIMQAFKAKRDMY
                     TIKLNSRKLVNYILTEYLDLDNAETITIIRLIDRKDKMSYAEFTALLSASISPTQREQ
                     DRVDDKLLALLDCKTLEQLPKEIASHESIQMLQKVTAHLQGQGITNIQFDVSLMRGFM
                     YYTDIVFEVFDTDPANNRSLFGGGRYDGLVGQFGVEPLPTVGFGMGDVTIRDFLETHS
                     LLPKLATNIHAAVLLVDITMHEALPFISRLRSEGAYIAVDSTDRKLDKKLKYVAKSGI
                     AYAIIIGQTELADNAITLKDMRRGTQEQHSPERIISIIMADSDAVMLD"
     gene            4531..>4712
                     /locus_tag="PKV52_01515"
     CDS             4531..>4712
                     /locus_tag="PKV52_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98925.1"
                     /translation="MATSNAESMDFFEKVGYSFGLWNESWEAFKLNVWTFVVLYLVPI
                     GLTILLVTFYLLPLAA"
BASE COUNT         1172 a         1008 c          930 g         1602 t
ORIGIN      
        1 cgacgatcaa ttttaacgta aattgcaccg ttaaccgctg tttcgaattc tagccacgca
       61 ccacgattag gaataacttt ggcaccataa gtgttcgtag agccgtgttg gtctgcagta
      121 aagaacacac ctggactacg gatgagctga ctcacgacta cacgctctgc cccgttaatc
      181 acaaacgtac cacgatcagt catccatggg taatcgccaa ggtaaatctc ttgctctttg
      241 acttcacctg taaccttatt gacaagttcg gcgttagcca ttagaggtgc ctcgtagctc
      301 acattgtttt cgcgagcttc tgcttcagac atcttaggat taccaaagta atagctttta
      361 aatgataatg ataatttcgt acctgtatag tcgtctattg gactaatctc tgcaaacagc
      421 tcacccaaac cttctttaat aaaccacgca aaactatcat tttggtggct aattaggttt
      481 ggaagggcta tgtcttcgct ttttgtaaaa aaagttctct tgcttgtagc aagcttcgtt
      541 ggcgctgatg ctttggccat aaagagcaca ctcctccgtt agtaatttta ttaatatttg
      601 gatccgtatt tttcgcgcga agttccctgg cgagggagtt tttggtactc gtattttgga
      661 ggcacacgag cacagcctag ccgacactac ttcttgatat atattctacg caaatatatc
      721 aataatgtaa agtttactac tatgtaattt ttacaattta cttgagtgta gtgatgattt
      781 tatccactaa accgtatttt ttcgcttctt ctgccgacat ccaataatca cgctcggcgt
      841 cttcttggat tcgcttgaca ggttggccgg tgttttttgc taatatttca ttaaggagat
      901 gttttacttt taacgattct tttagatcta tttccatatc tgttacttta ccgcgtgtac
      961 cgctgctggg ttggtgaatc atgactttgc tgtgtggcaa aaccatacgc ttgcctttag
     1021 tgccactact aagtaaaaag gcacccatac ttgcttgcaa gccaatgccc acagtctgta
     1081 catcgttctt tacgtattgc atggtgtcat aaatggccat gccatcatac acgctaccac
     1141 cagggctatt tatgtaaaaa attatatctt ttttagcatc ttctgcagcc aaaaataaca
     1201 tttgtgcaac cactaaatta gcagtgtgtg ggttgacgtc ttcgcccaaa aatataatgc
     1261 gttctttaag taaacggcta taaatatcgt aggcgcgctc gacgcggcca tctgattcaa
     1321 ttacggttgg aataagcata ctgtgcatta gattatcata caccacccat tagcactcgt
     1381 caatatagag tgctagttat tctgctagtt ggctttttag gcgtgcaatg ccttcgagca
     1441 tgttttctgc gtaactacca gattcaatag tttctcctat tttttgaggg ccatcttcgc
     1501 tgtcaataaa tacatctaca ctgtagtcat catcacctgc gataaatcct atcagcttgc
     1561 ctttactata caggtcaaat acatcgccat ttgtagggta aaaattacgt cggcaaaaaa
     1621 atgtgctgcc tgcttctaaa agctttcttg cttcgttaag ataggcatct atagcctctt
     1681 cactaagagg gctgtgagca cgtataaact tttcaaatat cacatatggc tctgcgtcac
     1741 catcataact aataacacgc tcgggggaca tctcttacct cgcacttctc ccacctttta
     1801 tagtagtgta ctacttagag ttctgaaaag aaacgagttt tgcaatagtt ttttcagtta
     1861 acaaacgagc agcaacttca cgtctgtttt ctggtttttc aagctcggac tgcatctttt
     1921 gatcgctttc atactggcct tttaagacct gtaagcgaat ttctagctct tctggagtta
     1981 tcgttacttt ttctatgtcg gcgagttcac taagcacgag gccggctttt aaccgttctt
     2041 gggcagcagg ttttaactct ttttccttat atgttgcttc gtctaggccg gtcgcttcaa
     2101 ggtattcttt gaacgtttgc ccacgataca cgaggttttg cttaaattct ttatctacaa
     2161 tttctatttg ttcagtcagc atggattcag gtagaggtac gtcagatgct gctactagtt
     2221 ctttaataag cgcatcttgg tattctctgt ctgcttggtg gtctcgctct tgttttagtt
     2281 gctttttaat atcagctttt agttctttta cggtactaac aggaccaact tttttggcga
     2341 actcatcatc tattggtggc aagattgttt cttctatctt ttttgccgtc actttaaacg
     2401 ttactttttt gccttgtaaa gcttttacac cgtaatcttt aggaaatgga atagtaaagt
     2461 tcttttggtc gtcttttttc atacccaaca cattatcttc aaagccaggt ataaacgtat
     2521 tggaaccgag cgctaaagga tagtcttggc cttttgcgcc attgactggt gcacctttcg
     2581 catcggtgcc gtcaaaatca atccacacac ggtcgccagt ttttgctgct cttgctactt
     2641 ctgcgtactc agccatttgt gtttgcaacc ggttaagaac ttcttctacg tcttttgccg
     2701 ttgcatcggt ggtctgtttc tttgctttta gctttttata gttacccagt ttaatagtac
     2761 ctatgacttc tatctctgcg gtaaattcta tatctgtata cggaacaaac tttttaagtt
     2821 ctatctttgg gtttgctact gggcgaatat tttcttgccg gagagccgtt tcatatagtg
     2881 catttaatac ttcgttgagc gtttcttctt gcaacgtatt aggattaagt tgtttttcta
     2941 ccaattctgc aggagcgtgc ccagggcgaa aaccttgcat ttttacttgt ggtgcaagtt
     3001 ttgcgacagc aatgtttttt gccgcagcga gttctgttgc tgtgccactt attgtgagta
     3061 ctatggttgt gtctgatggg ttggttctaa taatcttcat atgggtactt actgtaccat
     3121 atctaggctt gtttaatcta gcataaccgc gtctgaatcg gccataatta ttgaaataat
     3181 acgttcaggt gagtgttgct cttgcgtgcc acgacgcata tctttgaggg ttattgcatt
     3241 atcggcaagt tcggtttgac caattattat tgcgtacgct ataccactct tggcaacgta
     3301 tttgagtttt ttatctagtt ttctatctgt actatctacg gcaatatacg caccttctga
     3361 acggagtcgt gaaataaatg gcagagcctc atgcattgta atatcaacga gcaggactgc
     3421 tgcatgtatg ttcgttgcga gcttaggcaa aagactatgc gtttctaaaa agtcacgtat
     3481 tgtaacgtcg cccataccaa aaccaactgt aggcaggggt tctacaccaa attgaccaac
     3541 cagtccgtca taacggccac caccgaacaa tgaacgatta tttgctgggt cagtatcgaa
     3601 tacttcaaat acaatatctg tgtaatacat aaacccgcgc atcagcgaaa catcaaattg
     3661 tatatttgta attccttggc cctgcagatg tgcagtgact ttctggagca tctgtattga
     3721 ctcatgactc gcaatttctt ttggcagctg ttccaatgtc ttgcaatcaa gcaaggctaa
     3781 cagtttgtca tcaacacgat cttgttcgcg ctgagtaggg ctaatactcg cgcttaagag
     3841 cgccgtaaat tctgcatatg acattttgtc ttttctatca atcaagcgaa ttatcgtaat
     3901 tgtttctgca ttatctagat ctagatattc tgtaagtata tagttaacga gttttcttga
     3961 atttaattta atggtataca tatcgcgctt agccttaaat gcttgcatta ttgcatcggc
     4021 gagcattatg acttctgtat ctgctgaatc atcttgtatg ccaaataagt ctacattgag
     4081 ttgccagtgc tcgcgcaggc gacctcgctg tggtctttca taccgccaca cattaggaat
     4141 gctaaaccaa cgtaatgggt acgctagttc ttggcgctgg gctgctacca ttctacttac
     4201 tgtaggggtc atttctgggc gtagtgttac agccctacca ccacggtctg taaaagcgta
     4261 tgtttgttcg ccgacaattt cttcgctggt tttcgcctgg tacagttcta agggttctag
     4321 tactggagca tcgtattgtt cgtacccaaa ccgcttgcat gtttctgccc atgtatcaaa
     4381 tatgtattgc cgaatacgga agtcttttgg ataaaaatct cttgtacctt tgtacggtgt
     4441 ggtgcttaat gccataacaa cccaagtata tcagatattg actgtattaa aataaggggt
     4501 tttaataagg ttataacata aggaggctta atggctacgt caaatgcaga aagcatggat
     4561 ttttttgaaa aagttgggta ttcgttcggg ctatggaacg aatcatggga agcgttcaaa
     4621 ctaaatgtat ggacctttgt cgtcttatac cttgtaccaa taggtcttac cattttgcta
     4681 gtaacatttt atttattacc acttgctgcg ag
//
LOCUS       DAOPLS010000041         4615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_20377_length_4615_cov_4.365351, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000041 DAOPLS010000000
VERSION     DAOPLS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20377_length_4615_cov_4.365351"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PKV52_01520"
     CDS             complement(<1..807)
                     /locus_tag="PKV52_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98926.1"
                     /translation="MDKSHIRPRLSAYQRNFAAPAAVNTHASVVADMPLVSPPRPFGS
                     VQPKSAPDFKVGAKTYKRSQIKRRALKGVAVFGIGILLLLLLLGVIGYVLNERYAGKA
                     LPYTYVGDISIGGLTQPEIKAALDKRAEEIRVTLTEGGLVRDVPVKQFGAQFDTQKAS
                     EDAITGFNPFSYLTKRTVTVPVQVNERYVDGYLRLHVANMQTDAENAEIVKAKKDLVI
                     VPETTGFRTSSAYVTEQLQKQLPTLDDPTVSLSAVTDKPSITQADLQDDID"
     gene            complement(996..1886)
                     /locus_tag="PKV52_01525"
     CDS             complement(996..1886)
                     /locus_tag="PKV52_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98927.1"
                     /translation="MTQNNHEQAPFHETDPTKLIPFNSFDINRHRIEQIAGIDGPIVD
                     ELAKVFGVEKPENGWDVASLGELISIVGPAKTLQDNIPAMEEILPGVDGKRFAIEWVK
                     QSGLLEPAFRNYENPDRLVPHLFETAVITGGVRNWMMRRAKVLIETLQNGTEILEVNL
                     VAGMREMRTAEGSDVLEGDTEASYMERVIKPLIESSTNVTVNLSTPETTSGNEIAAEV
                     AKITKGAESVLVACNAGNWIQNAGQIRRAMDEDKNKVFVVSDTFPVAENDEPPAEAQN
                     PLTAVGIIGRNLQELKRQTQ"
     gene            complement(1960..2658)
                     /locus_tag="PKV52_01530"
     CDS             complement(1960..2658)
                     /locus_tag="PKV52_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98928.1"
                     /translation="MAKRRERDPNGIIFNGHDIGIGSDRLGNELYEAVYELATRVSPD
                     RPYVQETAIEPYEVTVHDQYFAMLTHGLRIQPRSFFLMTNDELRHNQNDPIISTFLSA
                     LEEHEPSDDPTQGAGALTYARRNSVVEQHRRLARGSIMTAMQETMSSMPELGHGHSDI
                     VLKSLGIVAVLGKYSSPKRLMVYPEGSSTWLLSHRINGVPRGYRYNMQDPNVRDAAQW
                     AIDYASGLINEIDN"
     gene            complement(2651..2902)
                     /locus_tag="PKV52_01535"
     CDS             complement(2651..2902)
                     /locus_tag="PKV52_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98929.1"
                     /translation="MNKRNEQFGVYYELTNSGVPETRLVETYGSRDDALTRAQQLNAE
                     EGLDDAPFEGAGDIERYTVMTVLKDLGNIATENESGIDG"
     gene            complement(2905..3039)
                     /locus_tag="PKV52_01540"
     CDS             complement(2905..3039)
                     /locus_tag="PKV52_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98930.1"
                     /translation="MKGATIADAAITSSETLYEIVEALRIENENLKTENELLKVLLGE
                     "
     gene            3207..3995
                     /locus_tag="PKV52_01545"
     CDS             3207..3995
                     /locus_tag="PKV52_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD98931.1"
                     /translation="MINLSAAGLNPTEAHVYQVLLSKKEWKPADLAKNVGESRTNMYK
                     ILDKLTALELAIKFDKSNKIHYRATNPTRLVQLAQEHRAQLEQSQKELETSTQSLLNE
                     FIKTHEQPGVRFFMGKDDIERVYQDQVNDQKPIYFMLSPKAIDFYGYAQMHKLRMLAV
                     KAKIPRFAITPENTRVPSNYEETDKAYLLTRTWLEANDYTASFEWGVYGDKVYFVTFG
                     EDALAMIIESKAMAEGFKQIFSIIDSRQRSKPSYARLPKTYKYT"
     gene            complement(3992..>4615)
                     /locus_tag="PKV52_01550"
     CDS             complement(3992..>4615)
                     /locus_tag="PKV52_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98932.1"
                     /translation="DRVLVLGGVHSSNLDRLKFVYEQIESNKIELFSEQSIVLLGGTR
                     PIQEGETSNGESMLTESDSLREAAVAHFGNLILSQVHLRLGLLPKSHNLVSKRVLRSD
                     KEVFNVINANAVSRPQGAPRHTTASTTLDWLTQDNIEDGARVLFVSGNPYIHRTTQDV
                     LSVIRHSGRSFDVESCGPAATPNLLVRRAYGELGRIIFSESVLQELN"
BASE COUNT         1235 a         1037 c          844 g         1499 t
ORIGIN      
        1 gtctatatcg tcttgtaaat ctgcttgggt aatagatggc ttatctgtca ctgcactaag
       61 actcaccgtt gggtcgtcta gggttggtaa ttgtttttgt aattgttctg tcacatatgc
      121 agagcttgtt ctaaaaccag tggtttctgg aactatcacc aaatcttttt tggccttcac
      181 gatttctgca ttttcagcat ctgtctgcat gtttgccaca tgtaaccgca aataaccatc
      241 tacgtagcgt tcgttcacct gcaccggtac cgtcacagta cgttttgtta agtaactaaa
      301 cgggttaaac cctgtaattg catcttcgct tgctttttgg gtatcaaact gagccccaaa
      361 ttgttttacc ggtacgtcgc gtactagacc gccttctgtt agtgttacac ggatctcttc
      421 tgctcgttta tctagagccg ctttaatttc tggttgcgtg agcccaccaa tagaaatatc
      481 accaacgtag gtatacggca atgccttgcc cgcataacgc tcattcaaca catagccaat
      541 gaccccaagt agtaacaata gcaataaaat accaatacca aaaactgcca ctcctttaag
      601 agctcttctt tttatctgtg agcgtttata tgtttttgct cctaccttaa aatccggggc
      661 gctctttggc tgtacagagc caaatggccg cgggggcgaa accagcggca tatcagcaac
      721 taccgacgca tgggtgttta cagctgcggg ggctgcaaaa ttgcgttggt atgcagatag
      781 ccgtggacgt atgtgcgatt tgtccacgaa ttacctcttt ttttacattt tgtcttggtg
      841 ttcttttgtg cgagcgtttt gttttttacg ctatataccc ttctagcata accattattt
      901 gcattcacgt caaataaaaa acgccccgtg tgagacgctt tttaattgtg catatatatt
      961 tttcgtatat tcaacagaat tttctggtat gtatactact gcgtttgccg ttttagctct
     1021 tgcaagttac ggcctatgat gccaactgct gttaaaggat tttgggcttc tgcaggtggc
     1081 tcatcgtttt ctgcaaccgg gaatgtatcg cttactacaa aaaccttatt tttatcttca
     1141 tccatcgctc ttcttatctg acctgcattt tgtatccaat tacctgcatt acaggcaaca
     1201 agcacagatt ctgcaccttt tgtgatcttt gccacttcgg cagcaatttc attaccgctg
     1261 gtagtctctg gagtagacaa attcaccgtt acgtttgttg atgattctat caaaggctta
     1321 atgacgcgct ccatgtagct cgcttctgtg tcgccttcaa gaacatcgct accttcagct
     1381 gtgcgcattt cacgcattcc ggcaactaga ttaacttcta aaatctcagt accattttgt
     1441 agtgtttcta taagtacttt tgccctacgc atcatccaat tacgaacacc cccagtaata
     1501 actgcggttt caaataagtg aggtactaat cggtctggat tttcataatt gcgaaaagct
     1561 ggttctaaaa gacccgactg tttcacccat tcaatcgcaa atcttttgcc atccacacct
     1621 ggtagtattt cttccatggc aggaatattg tcttgcagtg tttttgctgg acctacaatc
     1681 gaaataagct caccaagcga tgcaacatcc catccatttt cgggcttttc tacaccaaat
     1741 actttagcaa gctcatcaac tattggacca tcgatacctg caatctgctc gatacggtgt
     1801 ctgttaatat caaatgagtt aaatggtatt aatttagtcg gatctgtttc gtgaaatggt
     1861 gcctgctcat ggttattttg tgtcatcgtt atattccttt cttaatttat acttgtgttt
     1921 gttttattac acttaattat taatatatca cacttatgct taattgtcaa tttcattgat
     1981 taatccactt gcgtagtcaa ttgcccactg tgcagcatca cgcacatttg ggtcctgcat
     2041 attataccta tagcccctag gaactccgtt gatacggtgt gataatagcc acgtactgct
     2101 accttctggg tataccatca gtcttttagg actactatac ttacccagca cagctactat
     2161 acctaaactt tttagcacaa tatcactatg cccatgccct agctcgggca tgctcgacat
     2221 cgtttcttgc atagctgtca taatactacc tcttgctaag cgcctgtgtt gctctacaac
     2281 actatttcgt cgtgcgtacg ttaaggcacc ggcaccttgc gtggggtcat ccgatggttc
     2341 gtgctcttct agcgcagaca aaaatgtaga tattattggg tcattctgat tatgtcttag
     2401 ctcatcattt gtcattaaaa agaaagatct cggctgtata cgtaacccat gggtcagcat
     2461 cgcaaaatat tgatcatgaa ctgtaacttc atatggttct atagctgttt cttgcacata
     2521 aggcctatcg gggcttaccc gcgtagcaag ctcatagaca gcttcgtata gttcgttgcc
     2581 aagtctatct gaacctatgc caatatcgtg gccattaaat atgatcccgt tagggtctct
     2641 ttctcttctt ttagccatct attccacttt cgttttctgt agcaatattt ccgagatctt
     2701 taagtacggt catgacggta tatctttcaa tatcgccagc accttcaaaa ggtgcgtcgt
     2761 ctaagccttc ttccgcattt aattgttgcg ctcgcgtgag tgcgtcatcc cttgaaccat
     2821 aagtttcaac taagcgtgtt tcgggtacgc cagagtttgt tagttcgtag taaacgccaa
     2881 attgttcgtt gcgtttgttc ataattattc tcctaataat acttttagta attcgttttc
     2941 ggtttttagg ttttcgtttt ctatacgcag ggcctctact atttcataga gtgtttcact
     3001 actggtgatt gcagcgtctg caattgttgc gcctttcata caaaaactcc tttataaacc
     3061 gattagtaat atagtattac gcattatata caatttttgt acttatgtca atagtatttt
     3121 cactattgtt ttaatttatt tatctgtttt tttataattg tgaattatta tttcacattt
     3181 taggtttttt gtgctatact acttgcatga taaatttaag cgctgcaggt cttaacccaa
     3241 cagaggcaca cgtataccaa gtattgctct ctaaaaaaga gtggaagcca gccgaccttg
     3301 ccaaaaacgt cggcgaatca cgcacaaata tgtacaaaat actagataaa ttaacagctc
     3361 tagagctagc tattaagttt gataaaagca ataaaatcca ttacagagcc accaacccca
     3421 cgcgcttggt acagcttgcg caagaacatc gcgcgcagtt agagcaatca caaaaagaac
     3481 tagaaaccag cacccaatca ctacttaatg agtttattaa aacgcacgaa cagcccggtg
     3541 tacgtttctt tatgggtaaa gatgatatag aacgtgttta ccaagaccaa gtaaacgatc
     3601 aaaaacctat ctattttatg ctttcaccta aagcgattga tttttatgga tatgcgcaga
     3661 tgcataaact ccgtatgctt gcggttaaag ccaaaatacc tcgctttgca ataactccag
     3721 aaaatacacg tgtgcctagt aattatgaag aaacagataa agcatactta ttaactagaa
     3781 cgtggttaga agcaaatgac tacaccgctt cgtttgaatg gggggtgtat ggggataaag
     3841 tctactttgt tacatttgga gaagacgcat tggcaatgat aatagaaagt aaagctatgg
     3901 cagaaggatt taaacaaatt tttagcatta ttgattctag acagcgcagc aaaccaagtt
     3961 atgcacgcct accaaaaacc tataaatata cttaatttaa ttcttgtaaa acgctttcac
     4021 taaatataat tcgtcctaat tcaccatagg cacgccttac gagcaagttg ggagtagctg
     4081 caggtccaca gctttctaca tcaaatgaac gaccggaatg tcttattaca gatagaacat
     4141 cttgtgtagt cctgtgtata tatggattac ctgatacaaa taacactctt gcgccatctt
     4201 ctatattatc ctgcgttaac caatctagcg ttgtagaagc cgtcgtgtgt cttggtgcgc
     4261 cttgagggcg cgatacagca ttagcattaa tgacattaaa cacctcttta tcactacgta
     4321 atactcgttt actcactaaa ttgtgactct ttggcaagag cccgaggcgc aaatgtacct
     4381 gagaaagtat taagttgcca aaatgcgcca cagccgcctc tcgcaagctg tctgactctg
     4441 taagcatact ttcaccgttg cttgtttcgc cctcttgaat aggtcttgtg ccaccaagca
     4501 gtactatact ttgttcacta aatagctcaa tcttgttaga ctctatctgc tcatacacaa
     4561 attttaacct atctaaatta ctggaatgca cacctcctag taccaatact cgatc
//
LOCUS       DAOPLS010000042         4599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_20490_length_4599_cov_4.726673, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000042 DAOPLS010000000
VERSION     DAOPLS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20490_length_4599_cov_4.726673"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKV52_01555"
     CDS             <1..347
                     /locus_tag="PKV52_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPD98933.1"
                     /translation="MRLGVYPILIKMSVDTDTPMGYIRTMKKENKSQAVRRLKLIEGQ
                     LRGLQKMVEEDKYCIDIITQASAIKAALSGVEDLLLENHLTTHVLEQVKNGEEEKATE
                     EILKVYKLKRKQ"
     gene            358..729
                     /locus_tag="PKV52_01560"
     CDS             358..729
                     /locus_tag="PKV52_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004050478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HPD98934.1"
                     /translation="MEDLLVIISATLLIGLIVWWFFGKRKSSETEAIMSNGRQTVEIT
                     VNGGYTPNTVVLKQGVAAELVFDRKDPSGCFSHVVFPDFGINEELPVNKQHPVVIDTS
                     KPGEYKYACGMNMFHGKVVIK"
     gene            729..2651
                     /locus_tag="PKV52_01565"
     CDS             729..2651
                     /locus_tag="PKV52_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HPD98935.1"
                     /translation="MNVKNRFIFSLIVSLPMLLEMILRPLFGFVLPGHAWTMFLLTTA
                     VMAVAAWPFIRTAWAAFKNHNANMDTLIAIGTSTAYVYSIYAMLVHQPVFFEIAAFVI
                     TFILLGQVFEEAMKGRASSAIEKLLGLQAKEAEVLRGGKLVRVPLAEVVAGDVLRVKP
                     GQKIAVDGLITEGSSTIDEAMVTGESMPVTKKAGDAVIGSTINKSGTFMFEATKVGAD
                     TLLAQIVDLVKRAQVSRAPIQKTVDKISNIFVPAVLIIAILTFVAWFVLLGADLVTAM
                     LFAVAVIIIACPCALGLATPTALMVGTGRGAKLGILIKSGEVLEAANDIQTVVFDKTG
                     TITIGKPVVTDIVGNEKSILSLAASLEHSSEHPLATAILEKAKALDIEVKNAKNFKAV
                     EGKGITATVAGEAAFIGNVKLLGKSSLDAKLEGEMERLQTEAKTVVIVGANNKPLGLI
                     AIQDTPKDTSATAIAALKNRGLRTVMITGDNQRVAKAIADEVGIDEVIADVLPSDKAD
                     HVKSLQQHGKVAFVGDGINDAPALAAADLGIAMGSGTDIAIESGGIVLVKNNLLDVVQ
                     ALTLSQKTFGRIKLNLFWAFIYNTLGIPVAAGLFVWAGLTLSPELAGLAMAFSSVSVV
                     TSSLLLNRTKLMTRVA"
     gene            2668..3540
                     /locus_tag="PKV52_01570"
     CDS             2668..3540
                     /locus_tag="PKV52_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HPD98936.1"
                     /translation="MTTKDIPKEFQAHCAVAGDLLDTRVAEEKGLVAEYEGEKYYFCC
                     QACKPTFLANPAKYTSGEQEVISLEFTDKEHVTDNIWGFHFTPSTVVSWQAGQYMRVE
                     IPHDSPDERGTKRWFTVSSAPFEQQITIVTRISESSFKQALANLHAGDTINMIDLPGG
                     DFVWTKGDNIPRVFAIGGIGITPLYSIVKQRQHDGLSLNATLLYANRTEAIPYRDYFD
                     SLAPLGLKVKYLVGRKLTTKDILAESTTDDTIFYLSGAKPMVRQLANNLTQEDVADDH
                     IISDEFPHYDETNY"
     gene            3551..4231
                     /locus_tag="PKV52_01575"
     CDS             3551..4231
                     /locus_tag="PKV52_01575"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HPD98937.1"
                     /translation="MKNKTTIITIVAAILVVAIAFVVGPLLNDKLGDNKNSHENMSMP
                     NESGNGSLVQKDSEDYKMYAALDGDDYDKAFMSGMITHHEGAVDMAELALTNAKRQEI
                     KDMAQDIIAAQNKEIDDMRTWQKQWGYVGSTDHSGMNHGSSGSTMNMADHMDMMMDEL
                     RGKTGDEFDKAFLEQMIMHHQGALNMAAPGQDNAKHQEIKDLTKAVVDAQTKEIKQMQ
                     AWQKEWGL"
BASE COUNT         1303 a         1028 c         1146 g         1122 t
ORIGIN      
        1 taatgcgact tggtgtatat cctattttaa tcaaaatgag tgttgacact gataccccca
       61 tggggtatat tagaactatg aagaaagaga acaaatcaca ggcagtacgt cgcctaaagc
      121 ttattgaggg tcagttgcgc ggcctgcaaa aaatggtcga ggaggacaag tattgtatcg
      181 acatcattac acaagcttcg gcgattaagg cggcgctatc gggagtagaa gacctactgc
      241 tggaaaacca tttaactacc cacgtccttg agcaggtcaa aaatggcgaa gaagaaaaag
      301 ctaccgaaga aatactaaaa gtatacaaac taaaacgaaa acaataagga ggtgtccatg
      361 gaagatcttt tagtaatcat cagcgctact ctactgatag ggcttatcgt gtggtggttc
      421 tttggtaaac gtaaatcaag cgaaacggag gcaatcatgt caaatggtag gcaaacagta
      481 gaaataacag taaacggtgg ctacacacca aatactgttg tattaaagca aggagttgcg
      541 gctgagttgg tatttgaccg caaagacccg tcgggttgtt tctcgcatgt cgtatttccc
      601 gattttggta tcaacgagga actgcctgtc aacaaacagc atcccgttgt tatcgacacc
      661 agtaagcccg gtgagtacaa gtacgcctgt ggcatgaata tgttccatgg aaaggttgtt
      721 atcaaataat gaatgtcaaa aaccgtttta tcttttcact tatagtatcg ctgccgatgc
      781 tgctagagat gatcttgcgg ccgctgttcg gctttgtgtt accgggtcac gcatggacaa
      841 tgtttttgct caccacggct gtcatggccg tcgctgcatg gccatttata cgcaccgctt
      901 gggcagcatt taagaaccat aacgccaata tggacacgct aattgcaatc ggcaccagta
      961 cagcatacgt ttacagtatc tacgctatgc tcgttcacca acctgttttc tttgaaatcg
     1021 cggcgtttgt tattacgttt atcttgctcg gtcaagtgtt cgaggaagcc atgaaaggcc
     1081 gcgccagcag tgccattgag aaattgcttg gcctacaagc caaagaagcc gaggtgctgc
     1141 gcggcggcaa gcttgtccgc gtgccactag ccgaagtagt tgcaggtgat gtattgcgcg
     1201 tcaaaccggg acaaaagatt gccgttgatg gtttgatcac cgaagggtcg tcgaccattg
     1261 acgaagctat ggtgacgggc gaaagcatgc cagtgacgaa aaaggctggc gacgccgtga
     1321 ttggttcgac catcaataaa tctggtacgt ttatgtttga agctacaaaa gttggtgctg
     1381 ataccctgct cgcgcaaatt gtcgatttgg taaaacgagc acaagtcagt cgcgccccaa
     1441 ttcaaaaaac agtcgataaa atctcaaata tatttgtgcc ggcggtactt atcatcgcta
     1501 ttcttacctt tgttgcttgg ttcgtattac tcggtgctga tctggtaaca gctatgttgt
     1561 tcgcagttgc cgtcatcatc atcgcttgtc cgtgtgcact cggactggcg acaccgactg
     1621 cgctcatggt tggtacaggt cgtggcgcaa agctcggtat tcttattaaa agcggcgaag
     1681 tcctcgaagc tgctaatgat attcaaaccg ttgtctttga taagacaggt acaatcacca
     1741 tcggtaagcc tgtcgtgact gatattgtcg gtaacgaaaa atccatactg tcactagccg
     1801 ctagccttga acactcatca gaacaccctc ttgccactgc tatcttggaa aaagctaagg
     1861 cgcttgacat cgaggtgaag aacgctaaaa actttaaggc tgtagagggt aaaggtatta
     1921 cggcgacagt agctggcgaa gctgcattta tcggcaacgt aaaattacta ggtaagtcaa
     1981 gcctcgatgc gaagctagag ggcgaaatgg aacgcttgca aactgaagct aaaactgtcg
     2041 tgattgtcgg tgcaaataac aaaccgcttg gtctcatcgc catacaagac acgccgaaag
     2101 atacctctgc taccgcaatt gcggctttaa aaaatcgtgg gttgcggact gtcatgataa
     2161 ctggcgacaa tcagcgtgtc gcgaaagcaa ttgctgatga ggttggtatc gatgaggtaa
     2221 ttgctgatgt gttgccaagt gataaagccg atcatgtcaa gtcattgcag caacacggca
     2281 aagttgcttt tgtcggcgac ggcatcaatg acgcgccggc gctggctgct gccgatctcg
     2341 gcattgctat gggtagtggt acggacattg ccatcgaatc gggcggtatt gtgctggtaa
     2401 aaaataattt actggatgtc gtgcaagctc tgacgcttag ccaaaagact tttggtcgca
     2461 tcaaactcaa tctcttttgg gcgttcattt acaacacgct cggtatccct gtagcagctg
     2521 gattgttcgt ttgggcgggc ttgacgctca gccccgaact tgcaggcctc gccatggcat
     2581 ttagctcggt gtcggtcgtt actagctcgc tactactcaa tcgaacaaaa ttaatgactc
     2641 gtgtcgccta agaaaaggag aactactatg acaaccaaag acataccaaa agaatttcag
     2701 gcacattgtg ccgtcgccgg tgacctactc gacacccgtg ttgccgaaga aaaaggtctc
     2761 gtcgccgaat acgagggcga gaaatactat ttttgctgcc aagcatgtaa gccgacattt
     2821 ctcgcgaacc ctgccaaata tacgagcggt gagcaagaag ttattagcct tgaatttacc
     2881 gacaaagaac atgttactga taacatttgg ggctttcatt ttacgcctag cacggtcgtg
     2941 tcgtggcaag ctggccagta catgcgcgtt gagataccac atgattcacc cgacgaaaga
     3001 ggtacgaaac ggtggtttac cgtgtcgtcc gcgccatttg aacaacagat aacgattgtg
     3061 acgcgcatat ctgaatccag cttcaagcaa gctttggcta acctacacgc tggcgatacg
     3121 atcaacatga tcgacttgcc aggcggtgat tttgtgtgga caaagggcga caatataccg
     3181 cgcgtctttg cgattggtgg tatcggcatc acgccgcttt atagtatcgt caagcaacga
     3241 cagcatgatg gcttatcgct caatgcaacg cttctctacg ccaatcgtac cgaagctata
     3301 ccgtaccgag actattttga cagccttgcg ccgctcgggc tcaaggtgaa ataccttgtg
     3361 ggtcgaaagc ttacgacaaa agatatttta gctgaaagta cgaccgacga cactatattt
     3421 tacctgtctg gtgcaaagcc gatggtgcgc caattagcca acaatctaac acaagaagat
     3481 gttgcggacg accacattat tagcgatgag ttcccgcact acgacgaaac aaattattaa
     3541 aggaggaaat atgaagaaca agacaacaat cattactatc gtcgcagcca ttttggtagt
     3601 agccatagca tttgtcgtcg gcccacttct gaacgataag ctcggcgaca acaaaaacag
     3661 ccacgaaaac atgagtatgc caaatgagag cggtaacggt tcattagtgc aaaaagatag
     3721 cgaggattac aaaatgtacg ctgcgcttga tggtgatgac tacgacaagg ctttcatgag
     3781 tggtatgatt acccatcatg aaggcgctgt tgatatggca gagctcgccc tcacgaatgc
     3841 caaacgccaa gagattaaag acatggcaca agatattatt gctgctcaaa acaaagagat
     3901 cgatgatatg aggacgtggc agaaacaatg gggttacgtt ggaagcaccg accatagcgg
     3961 tatgaatcat ggctcgtcag gttcaacgat gaatatggcg gatcacatgg acatgatgat
     4021 ggacgaattg cgtggcaaga ctggcgatga gttcgataaa gcattcctag aacagatgat
     4081 tatgcaccac caaggcgcac tcaatatggc cgctccaggt caagacaatg caaaacatca
     4141 ggaaattaaa gaccttacca aagctgttgt agacgcgcaa acaaaggaaa taaaacaaat
     4201 gcaggcatgg cagaaagagt gggggctgta aggtaaatag tatttagtga cgcatgtttt
     4261 tacttagcgt agctaggctg tatctagtat agtcatacca gatacagtct attttttatg
     4321 ctattcgatt gttggctaac cgctaaactt tcgggtcgag ggcgcaaagc gcggcggctc
     4381 tgccgccaga gcgaagcctc gacgcctctg ccatttatgg cagtcgcccc accagaaaac
     4441 acccttttca actaaaaaag aaattcggcg ggggaaataa aaaataattc aaagggtttt
     4501 tctggtgggg cgagcgggcg tttcacgccc gcaggcgtcg gggctgagct tagggatttt
     4561 ctcggaaatt ggttcggact tcggcgtaaa atcgcacca
//
LOCUS       DAOPLS010000043         4572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_20648_length_4572_cov_4.285809, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000043 DAOPLS010000000
VERSION     DAOPLS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20648_length_4572_cov_4.285809"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PKV52_01580"
     CDS             complement(<1..1158)
                     /locus_tag="PKV52_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPD98938.1"
                     /translation="MAKKTTTKKVKQTTFTKNDAKLLPKFSLFFFDRPRFTAALLLFI
                     FAFGVLSYTTLLKREGFPSIDFPVASINGTYIGDAEKVDTSVAKPISDAAMKQQGVAT
                     VQSQAFDNFFNVFVRYEESVNAQEAAAQLESTLKTENIIPEQAQVTVSAPYISPAGRQ
                     LEPVDIGISVYGKANQTTEQLVASAQLFADELNQKNLSTVKKAYVIDPFQQATNPFTG
                     QSQIIQRTFDAYSQREANQSTLYPSVLVGVTKTDSADLLQFDDEVNAAVGEIVANQSD
                     TSTSAVVSASFAPDIRDNISELQRELLIALVAVLVVGALVIALRASFITVIALIVIIF
                     GVIGLLYVLGYTLNVITLFALILSLALIVDDTIIMIEAIDAQRRRQKSARKA"
     gene            complement(1192..2961)
                     /locus_tag="PKV52_01585"
     CDS             complement(1192..2961)
                     /locus_tag="PKV52_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD98939.1"
                     /translation="MQSIWRIITLSKELWRYYVAISIFTVVLSIVGLAMPMLSGWAID
                     EMQKGTSADVQYMVMLALGILAIEVVATIGNNISGYWGDQLSMKLYKILSFRYYQHLL
                     DLPQTYFDTELSGKIINRLNRSINQITSFMQMLSNNFLQFIFSTIFALAIVAYYSWQV
                     ALLLGVLYPIYIFLTMRTSNTWQNYQKDKNLNADIASGRFAESVGQIKVVKSFIQEKY
                     ELSIFSKYIDKAVAINVPQSKYWHTKDVQRRLILNIIFFLVYLFIFVQGAKGVFTPGQ
                     AVALILYAMQIRIPIFTISFLVDNTQRAIADSKDYFEVMAIQSDITDAPDAKVFTSKK
                     PTIVFDSVSFGYGDKQVLKDISFHIQNGQKVALVGESGEGKTTLTNLLLRFYEPSSGC
                     ITVAGQDISSVTQKSLRKNIGVVFQEPALFSGTIKENIAYAHQNATEEDIIKAAKNAN
                     AHEFISKFEKGYQTEIGERGLKLSGGQKQRIAIARALLKNAPILILDEATSSLDSKSE
                     VAVQQAMDKLMKGRTTIIIAHRLTTIAHVDLIITLKNGQVDEIGTPQELSKTNGIYAQ
                     LLTISHRQDENAKKRLKSFELSM"
     gene            complement(3003..>4572)
                     /locus_tag="PKV52_01590"
     CDS             complement(3003..>4572)
                     /locus_tag="PKV52_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD98940.1"
                     /translation="LLFGAVTILFDVIRRITYYYDRKFSEQVNIYVSTLYSSKVSSFT
                     QEQLDNPNLQTSLSMASRELYTVQGAADTLQNIASSIAAYILAVVVVWQYAWGIAVLL
                     FVLIPILAVSNYYQTKRSRKSWEESTIHWRISWGLFNYITDPLRLFEIRIMGARDSIL
                     KLREKHLRKEMDIEMQAVRKNAFFSLFEDISSPLIEVGTRIWAILLVAGGKLAFDQFL
                     FVIGLIQQASSQTFILGYNISRAQETYLATAALKDVMDIPDAPDGTEKIQIGGGGIAI
                     ELRDISLKYQNGTQALKNVTLSISAGQKVAIVGENGAGKTSLLRIIAKQYEPTEGTLI
                     INGIATKQLSRESLYKHTSVLSQDYYLFDDLTIKQNLEVVAGRTLTKDDILAALDVVH
                     LKQKILSLPHQLDTRLDKSYKDGADLSGGQRQRLSIARTLLKPFDLLILDEPTSAIDA
                     KAERSIFNSIFEKSASATVLIVSHRFATVRKADYIYVVDDGHIIEQGTHKELMAQNGH
                     YAELYTIQAQDFMD"
BASE COUNT         1280 a          958 c          890 g         1444 t
ORIGIN      
        1 ggcttttctg gcactctttt gccgcctacg ctgagcgtcg atggcttcaa tcatgataat
       61 agtgtcgtca acaatgagcg ctaaacttaa gataagtgca aatagtgtta tgacattgag
      121 ggtgtagcca agcacgtata acagtcctat cactccaaag ataatcacaa tgagggcaat
      181 aaccgttata aaggatgccc gtaatgcaat cacaagtgct cccacaacca acacggctac
      241 aagtgcaatc agtagttcac gctgaagttc agaaatatta tctctgatat cgggtgcaaa
      301 actggcactc acaacagcac ttgttgacgt gtcagattga ttggcaacta tctcacctac
      361 agccgcattt acttcgtcat caaattgaag caaatcggct gaatctgttt tagtgacacc
      421 tacgagtacc gatggataca gtgtactttg gtttgcttct ctttggctat atgcatcaaa
      481 cgtcctttga atgatttgcg attgccctgt aaacggattg gttgcttgct ggaaaggatc
      541 tatgacgtaa gctttcttaa ctgttgataa gtttttttga ttaagttcat ctgcaaatag
      601 ttgggcagat gcaaccaact gctctgttgt ttggttagcc ttaccataca cagaaatacc
      661 aatatccaca ggctctagtt gtctgccggc agggctaata tacggtgcac ttaccgttac
      721 ttgcgcttgc tcaggaatta tattctctgt ctttagtgta gactctagct gtgctgcggc
      781 ttcttgagca ttcactgact cttcataccg tacaaatacg ttaaagaagt tatcaaaggc
      841 ttgtgattgg acggttgcca cgccttgctg tttcatagca gcgtcagaaa tcggttttgc
      901 aacagaagtg tctacttttt cggcatcacc aatgtaagta ccattaatgc tggcgaccgg
      961 aaaatcaata cttggaaagc cttcacgttt tagaagggta gtgtaactta gtacgccaaa
     1021 ggcgaaaata aacaacaaca atgccgcagt aaatcgtggc ctatcaaaaa agaataggct
     1081 aaattttggc aataatttag catcattttt agtaaaggtc gtttgcttga ccttttttgt
     1141 cgtagttttt tttgccatac tcccccttaa ttacttaaca atattaacaa attacatgga
     1201 taattcaaaa gattttaatc gctttttagc gttttcgtct tggcggtggc taatggttaa
     1261 tagttgtgcg taaataccat tcgttttact aagttcttgc ggtgtaccaa tttcgtctac
     1321 ctgcccgttc tttagcgtga ttattaaatc aacatgggca atagtcgtga gccggtgagc
     1381 aataataatc gtcgttctac ctttcataag tttatccata gcttgctgta cggctacttc
     1441 actcttgcta tctaggctgc tggttgcttc gtcaagtatc agtattgggg cattttttaa
     1501 gagtgctctt gcaatggcta tgcgctgctt ttgaccaccg cttaatttta agccacgttc
     1561 accgatttct gtttggtacc ccttttcaaa tttactaata aattcgtggg cattggcatt
     1621 tttagcggct ttaataatgt cttcttctgt agcattttgg tgagcatacg caatattttc
     1681 tttaatagtg ccactaaata aggcaggctc ctgaaaaact acaccaatat ttttacgtaa
     1741 tgatttttga gtaacactac ttatgtcttg gcctgctact gtaatacacc cgctcgatgg
     1801 ctcgtaaaag cgtagtagca aatttgtcag tgtggtctta ccttcgccac tttcgccaac
     1861 aagggcaact ttttgaccgt tttgtatatg aaaagaaatg tctttaagca cctgcttatc
     1921 gccatagcca aagctcacgg aatcaaaaac aatggtgggt tttttactgg taaacacttt
     1981 tgcatcaggt gcatctgtta tgtctgattg tatcgccata acttcaaagt aatctttact
     2041 gtctgcaata gcccgttgtg tgttgtctac caaaaaacta attgtaaata tggggatcct
     2101 tatttgcatc gcatacaata taagagcgac cgcttggcct ggagtaaaaa cccctttagc
     2161 tccttgcaca aaaataaata aatacaccaa aaagaaaata atattaagta tcagccgccg
     2221 ctggacgtct ttggtgtgcc aatatttaga ttgaggtacg ttaattgcta cagctttatc
     2281 tatatactta ctaaaaatac taagttcgta cttttcttgg ataaagcttt taacaacttt
     2341 gatctgccca acactttcgg caaaccgacc gctggcaata tcggcattta aatttttgtc
     2401 tttttggtag ttttgccagg tgttactggt gcgcattgtt aagaatatat atattgggta
     2461 taacacacct aagagtaatg caacttgcca actataatac gctacaatgg caagggcaaa
     2521 aattgtacta aaaataaact gcaaaaagtt attactaagc atctgcataa aactagtaat
     2581 ttggtttatc gagcgattaa gtcgattgat gattttacca gaaagctccg tatcaaagta
     2641 cgtctgtggc aaatctagca aatgctggta ataccgaaaa ctcaatattt tatataattt
     2701 catcgatagc tggtcgcccc agtaaccgct gatattattg ccaatggttg caactacttc
     2761 gatagctaaa atacccaatg ctaacatgac catatactgc acatcggcag acgtgccttt
     2821 ttgcatttcg tctatggccc agccagaaag cattggcatg gcgagcccaa ctatactcag
     2881 cactacagtg aaaatactaa tcgctacgta gtagcgccat agttctttac taagggttat
     2941 tattcgccaa attgactgca tatctcatca tagtatacgg catcaatttg ccggcttaaa
     3001 cgctaatcca taaaatcttg tgcttgtatt gtgtacagtt cggcgtaatg accattttgc
     3061 gccattaatt ccttgtgtgt tccctgctct attatgtgtc catcatctac aacgtatatg
     3121 tagtcagctt tacgaaccgt cgcaaaacga tgcgacacaa tgagtacagt ggcgctggcg
     3181 cttttttcaa agatactatt aaatatactg cgctcggctt tggcatctat tgcagaggtt
     3241 ggttcatcta atatcaataa atcaaacggt tttaacaatg ttctagcaat agataagcgt
     3301 tggcgttggc caccagataa gtctgctccg tctttgtatg atttatcgag ccgtgtatcg
     3361 agctgatgtg gcaggcttag aatcttttgt ttaagatgca caacatcaag agctgctaaa
     3421 atatcatctt tagtgagcgt cctaccggcc actacttcaa gattttgctt tatggttaga
     3481 tcatcaaaca aataataatc ttggctaaga acagatgtgt gtttatacag gctttcgcgg
     3541 ctcagttgct tggttgctat gccattgatt attagtgttc cctcagtggg ttcgtattgt
     3601 ttggcaataa tccgcagtag cgacgtctta ccggcaccgt tttcacccac aatagccacc
     3661 ttttggcctg cagaaatgct tagtgttacg ttttttagag cctgtgtacc attttgatac
     3721 ttcagagata tatcgcgtag ctctattgct atgccaccgc cacctatttg aatcttttcg
     3781 gtgccatctg gtgcatcggg gatatccata acatctttaa gggcagccgt agcaagatat
     3841 gtttcttgag cccgtgatat gttatagccc aatataaagg tttgcgaact agcttgttgt
     3901 atgagtccaa tcacaaataa aaactggtca aaggccagtt taccaccagc cacaagtagt
     3961 attgcccaga ttctggtgcc aacttctata agtggagaag atatatcttc aaataaagaa
     4021 aagaaggcgt ttttgcggac agcttgcatc tctatatcca tttctttacg taagtgtttt
     4081 tcgcgtagct ttaatatgct gtcgcgggcc cccataatac gtatctcaaa cagtcgcagc
     4141 ggatcagtaa tataattaaa taatccccaa gatatacgcc aatgtatggt actttcttcc
     4201 cacgatttac ggctacgttt tgtttggtaa taattactaa ccgctagtat tggtataagg
     4261 acaaaaagta gcacagctat accccatgca tactgccaca ccactactac ggcgagtata
     4321 tacgccgcaa tgcttgaagc aatgttttgc aacgtatcgg cagctccctg cacggtgtat
     4381 aattccctac tcgccatgct caaagaagtt tgcaaattcg gattatctag ttgttcttgg
     4441 gtaaacgaag atactttact gctatacagc gtgctgacat aaatatttac ttgctcggaa
     4501 aactttctgt cgtagtaata ggtaatacgc cgtattacat caaatagtat tgttaccgct
     4561 ccaaataata gt
//
LOCUS       DAOPLS010000044         4438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_21468_length_4438_cov_4.486881, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000044 DAOPLS010000000
VERSION     DAOPLS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21468_length_4438_cov_4.486881"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /gene="rpmH"
                     /locus_tag="PKV52_01595"
     CDS             complement(<1..132)
                     /gene="rpmH"
                     /locus_tag="PKV52_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPD98941.1"
                     /translation="MPKRTHQPKTRKRARAHGFMKRMSSRAGRLTLKRRRLKGRVKIS
                     "
     gene            454..1842
                     /gene="dnaA"
                     /locus_tag="PKV52_01600"
     CDS             454..1842
                     /gene="dnaA"
                     /locus_tag="PKV52_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPD98942.1"
                     /translation="MQDDTLWQAILGEVELSVSRGSYVTWFKNTRVLRSEDNKVVIGV
                     ANIFTKQQLEKKFKPLLDELVVKYANQGTVLEFKIHAGISPPTSKKSNQDDTVVVTQT
                     AKPQTQTTISQQNGSNSVTHSYRQGLNERYTFDSFIVGSNNELAYAACQAISHNPGTK
                     YNPMFVYGGVGLGKTHLIQAVGNGVLANNPKARVVYITSEQFVQEFLDAIRYKKITDF
                     AGHYRSADVLIVDDMQFIAGKEKTQEEFFHTFNALHQANKQIIISSDKPPKSIPTLEE
                     RLRSRFEWGMIVDIGTPDFETRCAILQNKAVQHEVELPQDVVEFLANHFQSNVRELEG
                     ALNQLLAFCEMRSLIPDVQIAAGLFGSIKSRPKHVSAKQIVERTAKQFQIGIDEILSP
                     KRDKDIVVPRQVAMYLLRSELHLSFPKIAHELGRKDHTTAIHSVEKIQKELHFDSVLK
                     DQVNEIKERLYA"
     gene            2100..3194
                     /gene="dnaN"
                     /locus_tag="PKV52_01605"
     CDS             2100..3194
                     /gene="dnaN"
                     /locus_tag="PKV52_01605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPD98943.1"
                     /translation="MKLQVTQENLSKALSNVAKIAMANRNPLPILNNVLIKTIDGRLS
                     LSATNLEIAITEKIGSKVKEEGAITVPARLMQEYISSLPGGVLDLELTDNKLHISTDQ
                     YSSTINGVVADEFPSFPELAESVSWNIPTKECKKGLHQVVFAASNDESRPVLTGLYLH
                     TVNGALFAAATDSYRLAEKKLIKTSQEISLLVPADAMQELLRILPDTIDNVSVTADAQ
                     QVLFKAGDIELVTRLIDATYPDYKKLIPRTYSTTATLARDELVSITKVSSLFARESAG
                     SVTLKVDDEEKVVSITSIALQLGENSSKAPAHVEGAGEVTLNSRYLLDALNAIGTKEV
                     TIGFNGKLEPFVITSAEAAGQTYLVMPLKS"
     gene            3232..3588
                     /locus_tag="PKV52_01610"
     CDS             3232..3588
                     /locus_tag="PKV52_01610"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPD98944.1"
                     /translation="MITSFTDLRVWQEGHGVVLSIHDACSRFPKEELHGLTSQLKRAS
                     ASIASNIAEGFGRSSTKVNKHFWIIADGSCYEVKNQLIIAKDLQFITKLEYKVLLHKA
                     NSTYKLLYGLLRPHNV"
     gene            3581..>4438
                     /gene="recF"
                     /locus_tag="PKV52_01615"
     CDS             3581..>4438
                     /gene="recF"
                     /locus_tag="PKV52_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPD98945.1"
                     /translation="MSDIYNPLSTINHLTSLQLQHFRSYEDFAVELSPAVNIVVGPNA
                     SGKTNLLEAVLLLSGASPYRASMQDVIMQEKPWARIEAMLANRKRSVSLERVGDIVKR
                     RYTVNEVHRQRLKFEDILPVVLFEPEDMRLLTGSPELRREFIDDILTTIDPQFASIKR
                     HYVRALMQRNNLLKQRASTIKDQVFVWNIRLSELAGYIVAARHALISGFNEQISDIYS
                     TVATKKTVVLVEYNTPFAISSYESELLSKLESNLAQDIERGFTSYGPHREDISLYIDN
                     TAAKVAASRG"
BASE COUNT         1460 a          924 c          894 g         1160 t
ORIGIN      
        1 agaaatttta acgcggccct taaggcggcg tcttttaagc gttaaacggc cagcgcggct
       61 agacatgcgc ttcataaagc cgtgtgcccg tgctcgctta cgagtttttg gttgatgagt
      121 tcgtttaggc ataatcaact gaatactata ccacgcacgc ataaattact caagtaccaa
      181 cagatacata agcattacga cgcttagtaa aaaatacacg aataacctag tttgcagcct
      241 cgtttgtcca catatttatg taagttatcc actatttctt tactgttgca tggcgttgtg
      301 aataactatg acctctgttt ttggtagttt cactataatt aagaaaatat ttcaaccaaa
      361 ctgtggaaaa ctattaacgc gtcctgtata gttagtagta atcgcactaa tagacagcaa
      421 cattaacgca atacagaggg gagcgtctag cacatgcaag acgatacatt atggcaagcc
      481 atacttggag aggttgaact gtctgtttct aggggtagtt acgtaacgtg gtttaaaaat
      541 acccgtgtgc tacgcagcga agataataaa gtagttattg gggttgcgaa catatttaca
      601 aaacaacaat tagaaaaaaa gtttaaaccc ctgctagatg aacttgtggt taaatatgca
      661 aaccaaggta ccgtattaga atttaaaatt catgccggta tatcaccacc tacgagtaaa
      721 aaaagtaatc aagacgatac ggtggtagta acacaaacag ccaagccaca aacccaaaca
      781 acaattagtc agcaaaatgg ctcaaattcg gttacccatt cgtaccgcca aggattaaat
      841 gaacgctaca cttttgatag ttttattgtt gggtcaaata acgaacttgc ctatgcagcc
      901 tgccaagcga tttcacacaa ccctggcacc aaatacaacc ctatgtttgt gtacggtggt
      961 gttgggcttg gtaaaaccca ccttattcaa gcggtaggta atggcgtgct cgccaataac
     1021 ccaaaagcgc gtgtcgttta tataaccagc gaacaatttg tgcaagaatt tttagacgcg
     1081 atacgctata aaaaaattac cgattttgca ggccattacc gcagtgccga cgtgcttatt
     1141 gtagacgaca tgcaatttat tgccggcaaa gaaaaaactc aagaagagtt ctttcacacc
     1201 tttaacgcac tacaccaagc aaacaagcaa attattataa gtagcgataa gccaccaaaa
     1261 tctataccca ccctagaaga gcgcctacgg agccgttttg agtggggcat gattgtagat
     1321 attggcacgc cagattttga aacccgctgc gctatattac aaaacaaagc cgtacagcac
     1381 gaagttgaac ttcctcaaga cgtcgtggaa tttttagcga atcactttca gagcaacgtg
     1441 cgcgaactag aaggcgccct aaaccaactg cttgcctttt gtgaaatgcg cagtctcata
     1501 ccagatgtgc aaatagcggc tggtttattt ggcagtataa agtcacggcc aaaacacgtt
     1561 tctgctaaac aaattgtaga acggaccgcc aaacagttcc aaataggcat agacgaaata
     1621 ctcagcccca aacgcgataa agacatcgtg gtgccacggc aagtagcgat gtaccttctt
     1681 cgtagcgagc tgcatcttag ttttcctaaa attgctcatg aacttggtag aaaagatcac
     1741 accacagcaa tacattcggt agaaaagatc caaaaagaat tacattttga cagcgtacta
     1801 aaagaccaag taaacgaaat taaggaacgg ttatatgcgt aatataggtt gtggattaat
     1861 ggtgcaaaga tatgtgtata aaaagaggat aagtctggtt acttttacac agtatacaca
     1921 ggccaaacaa acgggctata atttcgcaaa cttataccct attatgtact cagccatttc
     1981 acagtatatg cacaataaat gtatgcaaat caccccagtt tatggttcgt tttacacagt
     2041 atacacaggc cctataacta ctactacata taatatattt aagaaaggaa actcgtaata
     2101 tgaagttaca agttactcaa gaaaatctta gtaaagcatt atctaatgtt gccaagattg
     2161 ccatggctaa tcgcaacccc ctaccgatac ttaataatgt acttattaag acaattgatg
     2221 gccggcttag tctatctgct accaacctag aaattgccat aacagaaaaa ataggctcta
     2281 aagtaaaaga agaaggtgct ataacggtac cggcacgcct catgcaagaa tatatttcta
     2341 gcttacctgg tggtgtgcta gatctagagc ttacagataa taaattacat atcagcaccg
     2401 atcaatacag ctcgactatt aacggtgtag tggcagatga gtttccgtcg tttccagaac
     2461 ttgcagagtc tgttagctgg aatatcccta ctaaagaatg taaaaaaggc ttacaccagg
     2521 tggtgtttgc tgcgagcaat gacgaatcgc gccctgtttt aacggggttg tatttacaca
     2581 cagtgaatgg agcactgttt gcagccgcaa cagatagtta ccgattggca gaaaaaaaac
     2641 tcattaaaac tagccaagaa atatcacttc ttgtgccggc agatgctatg caagaactct
     2701 tacgaatttt gccagatacc atagataacg tgtctgtcac agcagacgca caacaagtat
     2761 tgtttaaagc tggtgatata gaactggtaa cgcgccttat agacgccacc tacccagatt
     2821 acaaaaaact tattccacga acctacagca caaccgctac gctagcccgc gatgaacttg
     2881 tgtctattac aaaagtatca agcctatttg cccgcgaaag cgcaggtagc gttaccctaa
     2941 aggtagacga cgaagaaaaa gtcgtaagta ttacctctat tgcattgcaa ttgggtgaaa
     3001 actcatctaa ggcacccgca cacgtagaag gcgctggtga agtgacgctg aattctcgtt
     3061 acctactaga tgcactcaat gctataggaa ccaaagaggt tactataggc tttaacggta
     3121 agttagaacc atttgttatt acaagtgcag aggccgctgg gcaaacttat ttagtaatgc
     3181 cgcttaaatc gtagactaga tattgaatgt tagatattgg atactagata agtgataacg
     3241 tcgtttacag atttaagggt ctggcaagag ggtcacggtg tagtgctttc tatacatgat
     3301 gcttgttctc ggtttcctaa agaagaactt cacggactta cttcacaact aaaacgagca
     3361 agcgcatcta tagcatctaa tattgcagaa ggatttggta ggtctagtac taaagttaat
     3421 aaacactttt ggataatagc agatggttca tgttacgaag taaaaaacca actaattata
     3481 gccaaagatc tacaatttat aactaaatta gaatataaag tactattaca caaagcaaat
     3541 agtacctata aactacttta tgggttactt aggccacata atgtctgata tctataatcc
     3601 attatctacc atcaatcatc taacaagcct gcaactgcag cactttcggt cgtacgaaga
     3661 ttttgcggtc gagctttcac ctgctgtgaa tattgtagta ggcccaaatg cgagtggtaa
     3721 aaccaattta ctagaagcag tcttgctctt gtctggtgcc agcccataca gagctagtat
     3781 gcaagatgtt attatgcaag aaaaaccttg ggcccgtata gaagccatgc ttgcaaacag
     3841 aaaacgaagt gtcagcctag aaagggtcgg cgacattgtg aagcggcgct acactgtaaa
     3901 tgaagtacat cggcagcggc ttaaatttga agatatttta ccagtcgtgc tgtttgaacc
     3961 agaagatatg cgcctactca caggctcacc agaacttcgc cgggaattta tagacgatat
     4021 tctcacaacc atagacccac agttcgcaag tataaagcgg cactacgtac gggcgcttat
     4081 gcaacgtaat aatttactca aacaacgggc atccaccatt aaagatcaag tatttgtgtg
     4141 gaatattcgg ctcagcgaac tcgcgggcta tattgtagcg gctcgccacg cactgataag
     4201 tggttttaac gaacaaatta gtgacattta cagtacagtt gccacaaaaa aaacagttgt
     4261 tttggttgag tataatacac cgtttgctat ttcatcgtac gaatcagaac tacttagtaa
     4321 actagaatca aatttagcac aagatataga acgtggcttt acgtcttatg gcccacacag
     4381 agaagatatt agcttatata tagacaacac tgctgccaag gtggctgcta gccgcgga
//
LOCUS       DAOPLS010000045         4291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_22485_length_4291_cov_3.458451, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000045 DAOPLS010000000
VERSION     DAOPLS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22485_length_4291_cov_3.458451"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKV52_01620"
     CDS             <1..1154
                     /locus_tag="PKV52_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD98946.1"
                     /translation="LSKKLRTGLTVFGIAIGIGAIFFLLSFGIGLQRLVTNQVIGNQS
                     IKTIDVSSPNAGLVKIDDVNTNRIKEIPNVDTVGQAYYFPGSFKISGSESDGIVYGVD
                     QPYQSLTYLNLIEGSLLGDETEKSGLIINKAALEAIGLAGKPADVIGKPIEVTIPLSN
                     ATEKAGTLNKELPIVGVIDSGSGAELFVSNTIFREAGVKNITQLKVGARNVEDVKTIR
                     AQIESLGLETSSPVDTLNEINQVFKFLNFILIGFGSIGMVVAVLGMFNTLTISLLERT
                     REIGLMVALGARSVDMRRLFVLEAMLLSFIGSVVGILGAYLIGRIVNTIMNVFALRRG
                     VRESFDLFASPPLMILAMVIFMTFVGLIVVYLPARRAEKINPIDALRRE"
     gene            1285..2457
                     /locus_tag="PKV52_01625"
     CDS             1285..2457
                     /locus_tag="PKV52_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98947.1"
                     /translation="MKNTSGNPESVCAVETECPISDTIAKMAINAAEIHDSSDRLATF
                     KELGKAAKAAEKICRACRMVSYMRGYIAPVNESAAINNETIEYPDVVLHEDPATLANE
                     TGATAPDIEDAPSWANPKPEAGHQFFLPNDRKEVQLIMQYFIQKKGEHVRPRQIVEHI
                     SNALGLSESAARQRWNKYVLPSLVSAGLITSEGYTSSKKFTWVGDVSSGVQQAEPDPT
                     ALNQTIPTPEIIAQRDPNLSQHDYAEEPDQESLEQDLTELTDWGLAVSKLPDDEQSSR
                     QWVPSFYIDGRRLLLEWDACYTLLGLANQPDGIDAVSLFEDLQHLDHRLTLKKVRSSI
                     GRCEAVFALSGNTKAIVKTINPDTNATHYALAGRSYTENADQEAIQDFLAHGRPVQ"
     gene            2613..2900
                     /locus_tag="PKV52_01630"
     CDS             2613..2900
                     /locus_tag="PKV52_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPD98948.1"
                     /translation="MAKLEALGLDKPTNGWESHAVCAQTDPEVFFPKKGGSTRKSKNI
                     CLSCPVQKDCLANALIHNYEHGILGGKSAGERRALNRKAKDIIAKKTDGPI"
     gene            3054..3752
                     /locus_tag="PKV52_01635"
     CDS             3054..3752
                     /locus_tag="PKV52_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HPD98949.1"
                     /translation="MLNNLINDTSKSKLNWLRAAVLGANDGIVSIAGLVVGVAGATSD
                     TNVIATAGIAGLVAGALSMAAGEYISVSTQRDAEKAYIANEKKELKEHPTEQLQELTN
                     VYKSKGLSTVTAKKVAKELTAKDALQAHLEVEFGLDEDDLTNPWHAAYASAISFTFGG
                     LIPVIAILATPQSVRIGITFISVLIALALTGYLSAKIGNAPVRRATIRVVMGGLAAMI
                     ITYGIGVLFGVSVA"
     gene            3754..>4291
                     /locus_tag="PKV52_01640"
     CDS             3754..>4291
                     /locus_tag="PKV52_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98950.1"
                     /translation="MKSIRPISAKELFSVQMALIMAIVLQTISWVLSPSLHFGPQYVV
                     FAIEIALVTIIGITAKRRHLENNIFHHTLSIILIGIITAANISSLVLVLNELIRSDRV
                     IAGYELLASAIAIFLTNIIVFSLWYWEIDSPGLSGKSWTKNDKDFQFTQQDLPHEFPG
                     WQPVFLDYLYLSVTNAVNF"
BASE COUNT         1334 a          810 c          927 g         1220 t
ORIGIN      
        1 tacttagtaa aaagttacgt acaggtctta cggtttttgg catcgcaatt ggcataggtg
       61 caatattctt cttattatct ttcggtatcg gtttgcagcg cctggtgacc aatcaggtta
      121 tcggcaatca gtctattaaa accatagatg ttagtagtcc taatgcaggg cttgttaaga
      181 tagatgacgt aaacacaaac cgcattaaag agattccaaa cgtagacacc gttggacaag
      241 cctactattt cccgggtagt tttaaaataa gcggtagtga gtctgatggc atcgtgtatg
      301 gcgtagacca accgtaccag agtttaacat atttaaatct catagagggt tcactcctag
      361 gtgatgagac tgaaaagagc ggtcttatta taaacaaagc agcactagaa gcaattgggt
      421 tagctggaaa gccagcagac gtcataggaa aacctataga ggttaccatt cccctttcaa
      481 atgctaccga aaaggcagga acactcaata aagaactgcc tattgtaggg gttattgact
      541 ctggtagtgg tgcagaactg tttgtatcga atactatttt tcgtgaagct ggtgttaaaa
      601 acataacgca acttaaggtg ggtgctcgta acgtagaaga cgttaaaaca atccgagcac
      661 aaatagagag tctagggctc gaaacttcat cacctgtaga tactttgaat gaaataaacc
      721 aagtctttaa gtttttaaac tttatcttaa taggctttgg aagcattggc atggttgttg
      781 ccgtccttgg tatgtttaat acactaacca tatctttgtt agagcggacc agagaaattg
      841 ggctcatggt ggcgcttggg gcacgatcag tagatatgcg tcgcttgttt gtgcttgaag
      901 caatgctctt atcgtttatt ggttctgtgg taggaatact aggcgcatat ttaattggta
      961 gaattgtaaa tacaatcatg aatgtatttg cactacgtcg tggtgttcgt gaaagttttg
     1021 atttatttgc ttccccccct ttgatgattt tggctatggt tatctttatg acttttgtag
     1081 ggctaattgt tgtgtactta ccagcccgta gagcagaaaa aatcaaccct atagatgcac
     1141 ttcgccgcga ataaccaata tttttctaaa gaatgtaaca aataacacga ttaacgagcc
     1201 atagcagaca tgttcatctt ataaaaattg acatattata ttttgtatgg tcataatatc
     1261 tattaaagct aataggagtt agaaatgaaa aatacaagtg gtaaccctga gtcggtttgt
     1321 gccgtagaaa cggaatgtcc cataagtgat actattgcaa aaatggcaat taacgcagca
     1381 gaaattcacg attctagtga tcgtttagca acgtttaaag agctaggtaa ggctgcaaaa
     1441 gctgccgaaa aaatctgccg ggcgtgccgt atggttagtt atatgcgcgg ctatattgca
     1501 cctgtcaatg aaagcgcagc tattaataat gaaactatag aatatccgga tgtagtttta
     1561 catgaagacc cagctacttt ggctaatgag accggtgcta cagcacctga tattgaagat
     1621 gcaccatcct gggctaatcc caaacctgaa gcaggtcatc aattcttttt acctaacgat
     1681 agaaaagaag tgcagctaat tatgcaatat tttattcaaa aaaaaggtga acatgtacgt
     1741 ccaagacaaa tagtagaaca cataagtaat gcattagggc taagtgaatc agccgctcgt
     1801 caacgttgga ataaatacgt tcttccgtca ctagtatcag caggcttgat tacatcagaa
     1861 ggttatacta gctcaaaaaa atttacgtgg gtaggtgacg tgtcaagtgg tgttcagcaa
     1921 gcagaaccag atcctactgc gctaaaccaa actattccta cacctgaaat tatcgcacaa
     1981 cgagatccaa accttagcca gcacgactat gccgaagaac ccgatcaaga atcattagag
     2041 caagatctta cagaactgac agattgggga ttggcagtca gcaaattgcc ggatgatgaa
     2101 caaagcagta ggcaatgggt tccgtcattc tatatagacg gtcgtagact attgctagaa
     2161 tgggatgcat gctatacgtt acttggctta gcaaatcaac cagatggtat tgacgctgtt
     2221 agcctgtttg aagatttaca gcaccttgat catagactaa cactaaaaaa agtaagaagt
     2281 agtatagggc gatgcgaagc cgtgtttgcg ctttcgggta acacaaaagc aattgttaaa
     2341 acaattaacc cagacacgaa tgctacacat tatgcactcg ctggtaggtc atatacagaa
     2401 aatgcagacc aagaggctat acaagatttt ttagcccacg ggcgccccgt acagtaaacg
     2461 gtgtgcacaa taaagaacga ctggtacgta ataaagacgt tagcaagaat cttcgagata
     2521 ctacaggtag ctcatttgac gaagctatcg aacctagcat ttccagaaag ccacacacag
     2581 ccccgctata tcctagtgtg gcacctagag atatggcaaa actagaagca ctcggtctcg
     2641 ataaaccaac aaatggctgg gaaagccatg ccgtatgcgc acaaacagat ccagaagtat
     2701 tctttcctaa aaaaggaggt tcaactagaa aatctaaaaa tatttgccta agctgtccgg
     2761 tacaaaagga ttgcttagca aacgcgctta tacacaatta tgaacatggt atcttaggtg
     2821 gcaaaagtgc tggtgagcgt cgcgcactta atcgtaaggc taaggatata atagctaaaa
     2881 aaacagacgg tcctatataa acttacacac atgtataagc agcgtacatt actaagagaa
     2941 gagttgtatt ttagaatata tgacgtgtag gcttcggcct gctttattgt cacctatccg
     3001 gcactataaa ctttgtatat taatgtgtag gactctgata ttatgtaatt gctatgttaa
     3061 ataatcttat aaatgatact tcaaaatcaa aattaaactg gctacgggct gcagtactgg
     3121 gtgcaaacga cggtattgta tctattgccg gtttggtcgt gggtgttgct ggggcgactt
     3181 ctgatacaaa tgtcattgca actgccggaa tagctgggtt agtagctggg gcgctttcta
     3241 tggctgctgg tgagtatatt tcagtcagca cacaacgtga cgcagaaaaa gcctatattg
     3301 ctaatgaaaa gaaagaatta aaagagcacc ctactgaaca attgcaagaa ttaacaaatg
     3361 tttataaaag caaaggccta tcaacagtaa cggcaaaaaa agtagccaaa gaacttactg
     3421 ccaaagatgc tttgcaagct catctagaag tagaatttgg tcttgatgaa gatgatctga
     3481 cgaatccttg gcatgctgcg tatgcgtctg cgatatcatt tacttttggt gggttaatcc
     3541 cagtaattgc cattctagcg actccgcaat cggtccgaat tggtattact tttatttctg
     3601 tacttattgc attagcttta acaggctatt taagcgcgaa gattggtaat gccccagtgc
     3661 ggcgcgctac aattcgtgtc gtgatgggtg gtttagcggc tatgattatt acgtatggta
     3721 taggtgtgct atttggagta tcggtggcat agtatgaaat ctattcggcc tatttctgcc
     3781 aaagagctct ttagtgtaca aatggcattg attatggcaa ttgttctaca aacgatatct
     3841 tgggtgctta gccccagttt gcactttggt ccgcaatacg tagtgtttgc aatagaaata
     3901 gcactcgtta ccataatagg tatcactgca aagcggcgcc atttagaaaa taatatattt
     3961 caccatacgc tctctattat attaattggc atcataacgg ccgcgaatat cagttcttta
     4021 gtgctcgttt taaatgaact tattcgttct gatcgcgtaa ttgctggtta cgaactactt
     4081 gcttctgcaa ttgccatatt tcttacaaat atcattgtat ttagcttatg gtactgggaa
     4141 atagatagtc ctggtcttag tggtaaatca tggacaaaaa acgataaaga ctttcagttt
     4201 acgcaacaag acttgccgca cgaatttccc ggttggcaac cagtatttct agactacctt
     4261 tatctttctg tcacgaatgc ggtgaatttt g
//
LOCUS       DAOPLS010000046         4285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_22546_length_4285_cov_3.090071, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000046 DAOPLS010000000
VERSION     DAOPLS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22546_length_4285_cov_3.090071"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..975)
                     /locus_tag="PKV52_01645"
     CDS             complement(145..975)
                     /locus_tag="PKV52_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98951.1"
                     /translation="MNEDKIYERFRKLSGKQRQIAANNIGEWQKIGESHRNFGKAILD
                     YAYPHSHERRDTNALPLPAHHLVSSLYQDIAEGAPVTNRVRRLVGKVLLPSLGIHLPE
                     ATLQTETAKTNYRYCSAAEIGFIDCLDQINDTEDLGQSRTSVYFYDEVPIIFRKSHDE
                     PTALMLEDASIDGLYVPQGTIVGVGPAMNTQPIGKKDFIGNCGVWSLEAYDVEEIHIT
                     PGRLSPWAHKHSSGLKPLFGVNNPRKKVVNPRRSGLVNSLRLSDFKKSSKKLRKKLTI
                     "
     gene            complement(1232..1657)
                     /locus_tag="PKV52_01650"
     CDS             complement(1232..1657)
                     /locus_tag="PKV52_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD98952.1"
                     /translation="MDTTSPKKIIIVEDNMDLAEIYRTRLELLGFQCYIAYNGITALY
                     FIQKEIPDLVLLDLMIPDISGGQVLETMRNSSWGKNIKVLVVSNLNETEAPSNLRQLG
                     IVGYQVKANMSLDQIDKLVIDALTPDQPEPQAATSPTIG"
     gene            1800..2042
                     /locus_tag="PKV52_01655"
     CDS             1800..2042
                     /locus_tag="PKV52_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98953.1"
                     /translation="MPRSASTLYDVYHDISNTSVMRMRITDQVMQRALAGHKNYKKNV
                     AKAFRERVKKDYGSDPGWVEFSDLDFVVIRELDAEV"
     gene            2158..2475
                     /locus_tag="PKV52_01660"
     CDS             2158..2475
                     /locus_tag="PKV52_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HPD98954.1"
                     /translation="MNTLLSPYGRINRATYWKTIIVLYVGYLLAVFLAAMVLESNVND
                     AAVFMAVIGLIFLAFAYFYVSLVLFLKRGRDAGNAVLWAAIGLIVPFGFIIVGILPSK
                     KTL"
     gene            2848..>4285
                     /locus_tag="PKV52_01665"
     CDS             2848..>4285
                     /locus_tag="PKV52_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98955.1"
                     /translation="MQSPLRAAPDILHDWDLTNAADYSYDDGIEVVSGAARLKAQNYT
                     SDAQTNALYHFDESGGAFIGDSSSNANNATLSNGNFASGNLNNAVSLNGTDTSISVPN
                     SPSLQLGQQQSIESWVKFNNNFTVSSSDRRNAVIDKGDYQVYFDNETGKLTYELANAN
                     ATDWTQAGGGWNVGGKRLVSSTVAIGNTVYAGIGNSVSDAEVWRWDGAAWTMIGGDGI
                     NNGWVDQTFEEVTSLATDGTNIYAGLGNTAGDGEVWVWNGSSWTKIGGDGINSGWGAS
                     TFENVPSLRFVNSQLYAGLGASANDAEVWRWNGSSWTKIGGDGINSGWAGGYESVYAL
                     TDDGTNIYAGLGNSTGDAEVWRWNGSSWTKIGGDGVSSSWNTSYESVRSLSYMGGQLY
                     AGIGDGTTDAEVWKYNGTTWSQIGGDGVSGSWNTNYESVYSLANDGSVLYAGLGSGNG
                     DGELWKYNGTTWSQIGGDGVNSSWSTNEG"
BASE COUNT         1211 a          804 c          995 g         1275 t
ORIGIN      
        1 tataattact ccagcaccaa aacgagcctc tagccgtttg atggtgtaaa cacttgggta
       61 tttattagca gacagttctt accaacgcat aaatacttaa ttaaatatta gctattaaat
      121 aaacatgcca attatgattt atagttaaat agtcagtttt tttcttaact tttttgatga
      181 ttttttaaag tcagacaaac gcagactatt taccagacct gatctacggg gattaacgac
      241 ttttttgcgt ggattattca caccaaagag tggttttaat ccgcttgaat gtttgtgcgc
      301 ccaagggctt aatcgcccag gagttatatg gatttcttca acgtcatacg cttctaatga
      361 ccaaacgcca caattaccta taaaatcttt tttacctata ggctgagtgt tcatggctgg
      421 tccgactccg actatagtac cttgtggtac ataaagacca tctatgcttg cgtcttctag
      481 cattaatgcc gttggctcat catgtgattt cctaaagata attgggacct cgtcatagaa
      541 gtaaacgctc gtcctacttt ggcccaggtc ttctgtatca ttaatttggt ctagacagtc
      601 tataaagcca atttctgccg ctgagcaata gcgataatta gtttttgcag tttcagtctg
      661 cagagtagct tctggcaggt gtattccaag tgagggtaaa agtaccttac caaccagacg
      721 tctcacgcgg tttgtaactg gtgctccttc tgcgatatct tggtataaac tgcttactaa
      781 atgatgcgcc ggaagaggga gtgcattcgt atctcgtcgt tcgtgactat gtggataagc
      841 atagtctaaa attgctttcc cgaaattgcg gtgagattcc cctatttttt gccattcacc
      901 aatattattg gcagctattt gtctttgctt gcctgaaagt ttacgaaacc tttcatatat
      961 cttgtcttca ttcataacta aataactgta caaaccacca tattaaaagt atataatata
     1021 acaaaacagt aataataaca aacttactac cttaaaagca ccctcattcc agcctagtaa
     1081 aaaaattata aaaatacata aatttaacac ggtcgtcgta aatatatgta ttttgattta
     1141 agtaggtgtt aagggtggat gaaatggttt aattctaagt atctttgtat atatacacag
     1201 tattaacggg gccacatatg ttgaagaatt attaacctat tgtgggcgaa gtggcagctt
     1261 gaggttctgg ctggtcgggc gtcagtgcgt ctatgacaag cttgtctatt tggtccagag
     1321 acatattggc tttgacctgg taaccaacta tgccgagttg ccgtagattt gatggagctt
     1381 ctgtttcgtt aaggttagat actacaagta ccttgatatt tttaccccag ctactgttac
     1441 gcatggtttc tagtacttgc ccaccactta tatctggaat cattaggtct agcagtacta
     1501 aatctggtat ttctttttga ataaaataaa gtgccgtaat accgttatat gctatatagc
     1561 attgaaagcc gagcagctct aaccgagtgc gatatatttc tgctagatcc atgttatctt
     1621 ctacaatgat gatttttttg ggtgatgtgg tatccatgat agtgcttatt ataaacgaca
     1681 ggttactaac ttaccaccgc aactactagc gcttgtgctt ggctagcgcc acaattaatg
     1741 gtataaatag cctgacacaa attgtgttgt actcctacct atgttaggcg gtggttctgg
     1801 tgccaagaag tgccagcacg ctgtatgatg tctatcatga catcagtaat acttctgtga
     1861 tgcgtatgcg catcaccgac caagtgatgc aacgagcgct tgcaggccac aaaaattata
     1921 agaagaacgt ggccaaggct ttccgggaac gagtcaagaa agattacggc tcggacccag
     1981 ggtgggtcga attcagtgat ctggactttg tcgtcatccg agagctagac gctgaagtct
     2041 agcacagagt acgggttata gggcggccga gcaatcggcc ccctaccctc tatatatatt
     2101 ttttggcaaa actatcatac atataaacaa aaactattaa gtgatatgat tatacaaatg
     2161 aatacactac tttctccata cggaagaatc aacagggcta cgtattggaa gactatcatc
     2221 gtactttatg tgggctattt attggcagtg tttttagctg cgatggtgct tgaatctaat
     2281 gttaatgatg cagctgtctt catggctgtg ataggtttaa tatttttagc gttcgcctat
     2341 ttttatgtta gtttggtact gttcttaaaa cgtggccgtg atgccggcaa tgcagtgttg
     2401 tgggcggcga taggcctgat agtgccattt ggttttatta tcgttggtat tttaccgtct
     2461 aaaaaaacac tgtagtatcg ttgattcaaa gggttacatg ctcatgcttt atgtggtata
     2521 attttaccta gatattcttt gtggagggga atcataaaaa ctacaaatat taatcgtaaa
     2581 aaaatacgaa aattgtatac atctatcaag aggtctatac agcactttag catgttattt
     2641 ttacggtatt ttagtgtagc gattattaaa tcgtatgtac ttatagccaa gtgtataagc
     2701 tatatacgac tccattacac aaaacctctt cgcgccaagt tgcttgttcg ttaccctgct
     2761 ttgcaaaagt gggatacatg gaagttcaga ccacacacca gaaatgctac gggtatactg
     2821 agcgcttttt taatagtgac actccttatg cagtcaccgc tgcgggctgc tccagatatt
     2881 ttgcacgatt gggatcttac caacgctgca gactatagtt acgacgatgg gatagaagtt
     2941 gtatctggcg cagctcgctt aaaagcccaa aattatacta gtgacgcaca aacaaatgct
     3001 ttgtatcatt ttgatgagtc tgggggtgca tttataggag acagctctag taatgccaac
     3061 aatgcaacac tcagtaatgg taactttgca tcgggcaatc taaataacgc agttagttta
     3121 aacggtacag acactagtat ctctgtgccg aatagtccga gtttacagct tgggcaacag
     3181 caaagtatcg aaagctgggt aaagttcaat aataatttta ctgtaagtag tagcgatagg
     3241 cgcaatgctg tcatagacaa aggtgactac caagtgtatt ttgataatga aactggtaaa
     3301 ttaacgtatg agctagcaaa tgcaaatgct acagactgga cacaagcagg tggtggctgg
     3361 aatgtcggtg gcaaacggtt ggtatctagt acggtggcca ttggtaacac ggtctatgct
     3421 ggtatcggta atagtgtttc cgatgctgaa gtatggcggt gggacggagc tgcatggacg
     3481 atgattggtg gcgatggcat caataatggt tgggttgacc agacctttga agaagtaacc
     3541 tcgcttgcta cagatggaac aaatatttac gctgggcttg gtaacacagc tggcgatggc
     3601 gaagtttggg tgtggaacgg tagctcttgg acgaaaatcg gtggcgacgg tatcaatagt
     3661 ggttggggag catctacttt tgagaacgtc ccttccctac gctttgtaaa cagccagtta
     3721 tatgcagggc ttggtgcgag tgctaacgac gcagaagtat ggcgctggaa cggtagctct
     3781 tggacgaaaa tcggtggcga cggcatcaat agtggctggg ctggtggtta cgaaagtgtt
     3841 tatgcgttaa cagacgatgg aacgaacata tatgctgggc ttggtaattc tacaggcgac
     3901 gcagaagtat ggcgttggaa cggtagctct tggacgaaaa tcggtggcga tggggtaagt
     3961 agcagctgga atacaagtta cgaatcagtg cgctcgctta gctacatggg tggtcagctt
     4021 tatgcaggca taggtgacgg cacgacagac gcagaagtct ggaaatataa cggtacaacc
     4081 tggagccaaa tcggtggcga cggtgtaagt ggcagctgga atacaaatta cgaatcagta
     4141 tatagcttgg cgaatgatgg gtcagtatta tatgccggtc taggcagtgg aaatggcgat
     4201 ggtgagctat ggaaatataa cggtacaacc tggagccaaa tcggtggcga tggtgtaaac
     4261 agtagttggt ccactaacga aggtg
//
LOCUS       DAOPLS010000047         4276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_22618_length_4276_cov_3.199479, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000047 DAOPLS010000000
VERSION     DAOPLS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22618_length_4276_cov_3.199479"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..96)
                     /locus_tag="PKV52_01670"
     tRNA            complement(4..96)
                     /locus_tag="PKV52_01670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60..62),aa:Ser,seq:gct)
     gene            complement(160..828)
                     /locus_tag="PKV52_01675"
     CDS             complement(160..828)
                     /locus_tag="PKV52_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPD98956.1"
                     /translation="MNILDTALSLVAPHQCVVCNAEGAVLCKSCAATLPTLPSICYVC
                     GKATKHNNPCQQHKSSSAPIAVYIATDYQGAIKKLIKAYKFDYKRSAAKDIAYFLQLN
                     IPYLDSDYVLTYVPATGSHIRERGFNHMQAVTKALAKMQKRKVIETLVRVTSVTQKGA
                     DRPTRKKQLANAFRPIAGHVAGKKLLIVDDVVTTGATIDATVKLLYKAGAQEVRVAVV
                     ARTP"
     gene            957..1427
                     /locus_tag="PKV52_01680"
     CDS             957..1427
                     /locus_tag="PKV52_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD98957.1"
                     /translation="MARRTADDDLLMEEELTAAFLGDEDLVSPAAAPAQPVAEEEEWD
                     ENEPILGQLAVDVYETKEKLVVKARTAGVNKNDLDVSIADNTLSIRGTLSAGGEEDVE
                     NYHLQECYWGEFSRSLVLPVPVKEDEIEAVLKDGVLTISFTKVQQDTVKKIQVL"
     gene            complement(1536..1955)
                     /locus_tag="PKV52_01685"
     CDS             complement(1536..1955)
                     /locus_tag="PKV52_01685"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPD98958.1"
                     /translation="MNRLKTLIIAAFTAVGMLAPLTVPAIAIAQSDIQSGLCGGANLQ
                     APNQAGDCTNINDEASEGVNDTVNLIINIFSWVVGVVSVIMIIYGGFRYITSGGDAGS
                     VTSAKNTILYAIVGLIIVALAQVIVKFVLGNVANTAS"
     gene            complement(2041..2412)
                     /locus_tag="PKV52_01690"
     CDS             complement(2041..2412)
                     /locus_tag="PKV52_01690"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPD98959.1"
                     /translation="MKAFLIGLAALATFAFVGPVLTTTTSYAQIKKGVEATCTGDQCA
                     GSAPAKVNTLVEDIINIFSWIVGVVSVIMVIFGGFRYITSGGDAGKVTSAKNTIVYAL
                     VGLVIAALAQVLVLFVLGNVT"
     assembly_gap    2539..2638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2639..2886)
                     /locus_tag="PKV52_01695"
     CDS             complement(<2639..2886)
                     /locus_tag="PKV52_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98960.1"
                     /translation="MKKLFLVIVAVFGLSAAALPALSLPIYASPVTDGINATCPDGNC
                     QQYDSEGNKIGVKAKANNLVQDIINIFSWIVGVVSVIMV"
     gene            complement(2900..3301)
                     /locus_tag="PKV52_01700"
     CDS             complement(2900..3301)
                     /locus_tag="PKV52_01700"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98961.1"
                     /translation="MKMFLTSLFATALLFLTISPLAMAASATEDLTEVCKNNPTSSLC
                     KGYDKGSDADPGDNAILILIKRIINILSFVAGALAVFMVIFGGFKYITSAGEPQKAAT
                     GRQTILYSLVGLVVVVLANRLILFAIAKLLN"
     gene            complement(3311..3589)
                     /locus_tag="PKV52_01705"
     CDS             complement(3311..3589)
                     /locus_tag="PKV52_01705"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98962.1"
                     /translation="MNILYTFSAIISSDYLPKTNTNSFGTILNYVLAIFGAVAVIMVV
                     YSGIQLIISQGNSEKVAQARRAIIYAVAGLVVIFFAQVIVLFIFNRVT"
     gene            complement(3592..4101)
                     /locus_tag="PKV52_01710"
     CDS             complement(3592..4101)
                     /locus_tag="PKV52_01710"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPD98963.1"
                     /translation="MQLVAKVLTSLALLLVPSMGIAVLGIVPSTVHVVAQSDNPENPR
                     QADGTEANIQAEGNKCRPGSGGLPLPTWYQYLPGQKVNGQCEIKTDGLGGTVIILILM
                     GIFDILLYVAGVIAVVMIIWGGFKLLTSDGEPQKIAAARTTLFNALVGMVIAVIASQI
                     VGFIAGGLA"
BASE COUNT         1225 a          898 c          842 g         1211 t
ORIGIN      
        1 atttggcgga ggaggaggga ttcgaaccct cgcaccaggt tgccccgatc taacgattta
       61 gcaaaccgtc cccttcagcc acttgggtac tcctccacta ctagtggtag ctgacagctt
      121 aaaattatag cagttagcag ctccttttaa aagaattttc taaggtgttc tcgcaacgac
      181 agccacgcga acctcttggg ctcctgcttt gtataataat tttactgtag cgtctattgt
      241 agccccagtt gtcactacat catcaactat aagaagtttt ttacctgcaa cgtgcccagc
      301 tatcgggcgg aacgcattcg ccaattgttt ttttctggtg ggcctgtctg cgcccttttg
      361 cgtaacagat gttacgcgca ctaatgtttc tattacttta cgcttttgca ttttggcaag
      421 tgcttttgta acggcttgca tatggttaaa accacgctct ctgatgtggc tacctgttgc
      481 gggtacatat gtaagcacgt aatctgagtc tagatagggt atattgagtt gcaaaaaata
      541 ggcaatatct tttgcagccg aacgtttata gtcgaatttg tacgccttga tgagcttttt
      601 aatagctcct tggtaatctg tagcaatgta tacggcaatt ggtgcagatg aacttttatg
      661 ctgttggcat gggttgttat gttttgtagc tttgccacat acataacaaa tacttggtag
      721 ggtaggcaac gtagctgcgc atgacttaca caaaactgcg ccctcagcat tacacacaac
      781 acattgatgt ggtgcgacta gtgatagggc ggtatctaaa atgttcatgt tgtaataatt
      841 atgttgtgaa tatagctatt aaagttgcta tctactctgt atgtcagtta taatctcatg
      901 taaagataaa aaatcaaatc ttaaaaagct gcaaataaat aatcgtggag ggtaacatgg
      961 cacgacgtac ggcagacgat gatctgttaa tggaagaaga gctaacggct gcgtttttgg
     1021 gtgatgaaga tctagtatct ccagctgccg caccagcgca accagtcgca gaagaagaag
     1081 aatgggacga aaacgaacca atcttaggac aattggcggt tgatgtatac gaaacaaaag
     1141 aaaaacttgt tgttaaggca cgaactgctg gcgttaacaa gaacgatcta gatgtgagta
     1201 ttgcagacaa caccttaagc attcgcggga cactatctgc gggtggcgaa gaagacgtag
     1261 aaaactacca cttgcaagaa tgctactggg gcgaattttc tcgtagctta gtactacctg
     1321 tgccagtaaa agaagatgaa attgaagctg ttttaaaaga tggcgttcta accattagct
     1381 ttacaaaggt acagcaagac acggttaaga aaattcaagt tttataagta gttgctaaag
     1441 taaaaaagac ttctgggtaa aactagaagt ctttttatat gtaaaaatcc cccttttgta
     1501 aaagggggat tacttaacgt ttggtgtgca gctatctagg atgctgtgtt ggcaacgttg
     1561 ccaagtacaa acttcacaat aacctgcgca agcgccacga tgataagacc tactattgcg
     1621 tacaatatgg tgttctttgc agaagtaacg ctaccggcat caccaccgct ggtaatgtag
     1681 cggaaaccgc catagataat catgatgaca gaaacaacac caacaaccca agaaaagata
     1741 ttaatgatga ggtttaccgt atcattaaca ccttctgatg cttcgtcatt gatatttgta
     1801 cagtcgccag cttgattggg tgcttgcaag tttgcgccac cacacaggcc actttgaata
     1861 tcactttgag caattgctat agcaggaacg gtcaatggag cgagcatacc tacagctgtg
     1921 aatgccgcta ttattaatgt ttttagtctg ttcatgtaaa ttccttattt aacgttagct
     1981 tatgatactt ttttaccata tattaatgtt tttgtaaata tataatcgtt cgttcactat
     2041 ttaggttaca ttaccaagca caaaaagcac aagtacttgc gcgagtgcgg cgattaccaa
     2101 accaaccagt gcatagacaa tggtattttt ggcgcttgtt actttaccag catcaccccc
     2161 gctagtgata taccgaaaac caccaaatat gaccataatg accgatacca caccaacgat
     2221 ccatgaaaag atgttgatga tgtcttctac cagagtgtta acttttgctg gtgcgctgcc
     2281 cgcacattgg tcaccggtac atgtagcttc gacacctttt tttatctgtg catagctcgt
     2341 agtagttgta agtacagggc ctacgaaagc aaatgttgcg agtgctgcta ggcctattaa
     2401 aaacgctttc atttttttac tcctttattt atctttaggt tacattacca agcacaaaaa
     2461 gcacaagtac ttgcgcgagt gccgcgatta ccaaaccaac cagtgcatag acaatggtat
     2521 ttttggcgct tgttacttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
     2641 cataatgacc gataccacac caacgatcca tgaaaagatg ttgatgatgt cttgcacgag
     2701 gttgtttgct tttgccttaa cacctatttt attgccctcg ctgtcgtact gctggcagtt
     2761 accatccgga cacgtagcat tgatgccatc tgttactggg ctagcgtata tgggtaaact
     2821 caatgctggc agggcagcgg cacttagtcc aaacactgct acgattacta aaaagagttt
     2881 tttcatttta tatcctttat tagtttaata actttgctat tgcaaataat atcaaccgat
     2941 ttgctaatac aacaaccact aacccaacaa gtgagtacaa tatagtctgc cgcccagttg
     3001 ctgccttttg cggttcacca gcgctggtaa tgtacttaaa accaccaaat ataaccataa
     3061 aaactgcaag cgcacctgct acaaaagata atatattaat tattctctta ataagaatta
     3121 gtattgcgtt atcgcccggg tcggcgtcac tacctttatc gtagccttta catagtgaac
     3181 tggttgggtt gtttttgcac acttcggtta aatcttctgt tgcgctcgca gccatcgcta
     3241 aaggacttat ggtcaaaaaa agtagtgctg tggcgaataa agatgtcaaa aacattttca
     3301 tatgtatatg ctacgtcacc ctattaaata taaacagaac aatgacttgg gcaaaaaata
     3361 ttacaaccaa ccccgcgacg gcatatataa tcgcacggcg agcctgggca accttttctg
     3421 aattgccctg cgaaataatc agttgtatac cagaatagac aaccataatg acggcaactg
     3481 ctccaaaaat agcgagaacg taatttaaaa tcgtgccaaa actatttgtg tttgtttttg
     3541 gtaaataatc agaactaatt attgctgaaa atgtgtataa aatattcata tttaggcgag
     3601 tcctccggca ataaagccaa ctatttgcga cgcgataact gcaattacca tacctaccaa
     3661 ggcattaaac agcgttgttc tggcagcggc aattttttgc ggttcaccat cgctcgtaag
     3721 taatttaaaa ccgccccaaa taatcatcac tacagcaata acgcctgcta catatagaag
     3781 gatatcaaat atccccataa gtattagaat aatgactgtt ccacctaggc catctgtttt
     3841 aatttcacat tgcccattta ctttttgccc cggcaagtac tgataccagg ttggaagagg
     3901 cagacctccg ctacctggcc ggcatttatt accttctgcc tgtatgtttg cttctgttcc
     3961 atctgcttga cgggggtttt ctggattatc tgattgcgca acaacatgca ccgtagacgg
     4021 tactatacct agcacagcaa tacccatact tggtacgagt aatagtgcta ggcttgtgag
     4081 aactttggca acgagctgca ttaaaacacg ccccccggta ttaaccactg tataaaggcg
     4141 tacaaaaaga agtaggccac taaaccaaat acaacgttcc atattttttc tttagccgct
     4201 tgtacgtttt gtgcgttgtc tcttgctgtc atgtactgta tgcctgccca cgcaatcatg
     4261 acagaaccac cagcta
//
LOCUS       DAOPLS010000048         4163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_23522_length_4163_cov_3.294304, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000048 DAOPLS010000000
VERSION     DAOPLS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23522_length_4163_cov_3.294304"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKV52_01715"
     CDS             complement(<1..371)
                     /locus_tag="PKV52_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98964.1"
                     /translation="MYKNASNIEYKKTGSPRPIMRIATGLNFSYGIVEATVGLMSGSS
                     AAFANGWHNFLDSISHGAHTSTHKKELSDEYVHKKVMRRRRLAATAIAAGAIMTGANA
                     AHSIANPEEAPLNVKALSAELG"
     gene            524..1012
                     /locus_tag="PKV52_01720"
     CDS             524..1012
                     /locus_tag="PKV52_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPD98965.1"
                     /translation="MDIVRRSPAGLVIKIIAIEISLEIIYLLLSTALVEVRSQAGESY
                     TLARAILSVMFLSLAITAVVTLVAQWANEGYYIKENELVVRRGIIHKTEISYPYANMQ
                     SVSVQQGFFGRLFNFGLVTIFIPTLGKDILFNEISAPKKFAQILKDHIPYAESGQFLI
                     RK"
     gene            1075..1755
                     /locus_tag="PKV52_01725"
     CDS             1075..1755
                     /locus_tag="PKV52_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD98966.1"
                     /translation="MMNKVNILVVEDNTDMRTAVSDYLTDNGYIVRSAPSAELAQEKN
                     YPAASDIAVLDISLPGITGLSFTRMLKEQGFDGPIIAITARDTVDDKIIGLEAGMDDY
                     LVKPFDLRELVARINAQLRTKGIQHDLAPIKTAHFKIEPKRHKFFIQDKEVKLTLVEF
                     RIMRKLMQNSHTTVSTQDIIESAWGDDAAITNPPIRIHISNLRNKINDPTLTIIQTIP
                     GVGYLLND"
     gene            1761..2774
                     /locus_tag="PKV52_01730"
     CDS             1761..2774
                     /locus_tag="PKV52_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD98967.1"
                     /translation="MDKVLATARKNLTFATVGVAIICALVASIAMYAYAEQRLQQNIS
                     AAKIGQLAQDNSELSAYIASQAPNHAHFETIAGIIQREDRNQITASLPFVLVAVIVMS
                     GLAGWWLSRRLLSPVKEAYLSQRRFMQDAAHELRNPLAAMSTMLQQAKNRPPAGKELN
                     TFIQSLGRQATHLSSITTDLLLLEHRDYPGTQEIDIAELLGDIVEELHHQAVAKHITV
                     NLKKPTTLNTKIDPQHFVYIAKNVLENAIKFSDKKGKPIDVQLTHSKTGWTLKVRDYG
                     VGIPKQDLPNITQRFYRAKNATNTDGTGLGMAIIDKFVSIYHGDVSITSTVGKGTTVI
                     VTL"
     gene            2844..3419
                     /locus_tag="PKV52_01735"
     CDS             2844..3419
                     /locus_tag="PKV52_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008281694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPD98968.1"
                     /translation="MESNKTAGIVAVVLAIAVAVGCIVWFANREDTTATNETSQSNMQ
                     PAEQQANRDIVVVASETESLSTLVTAVKAANLVETLQGAGPFTVFAPTNDAFAALPAG
                     TLDTLLKPENQDQLKSILTYHVVPGTVLAGDLTDGQVITTVQGGTLTVKIADGMVYLV
                     DATGNQVKVEKADVNADNGVVHVIGGVLLPQ"
     gene            3625..3801
                     /locus_tag="PKV52_01740"
     CDS             3625..3801
                     /locus_tag="PKV52_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98969.1"
                     /translation="MDFIRDLLGSVQNVSQQAIPEPQELMDIALGSTSIDESIQQFTE
                     NTDTVQTITDSLKR"
BASE COUNT         1310 a          884 c          860 g         1109 t
ORIGIN      
        1 cctagttcag ctgataatgc cttcacattt agtggggctt cttcagggtt tgcaatagag
       61 tgggcagcat tcgcgccagt cataatagcc ccagctgcaa tagccgttgc tgccaatcga
      121 cgccttcgca ttactttttt gtgaacatat tcgtcgctta actctttttt gtgtgttgat
      181 gtatgagcac catgtgaaat tgaatctaga aaattatgcc agccatttgc aaatgctgcg
      241 ctactaccgc tcataagacc tacggtcgcc tcgacaatac catagctaaa gttcaaacct
      301 gtcgctatac gcataatcgg tcttggagag cctgtttttt tatattctat attactagca
      361 tttttataca taccattata tagtatgtgc tcaaaaagta gcgattacaa gcgcaagcat
      421 tcatatacgg tatagctttt tgcttaggtt gagtagcagc tttacaggtc cttaacaagc
      481 aattctttat agttagtaat agtcacgtaa cagaaagggt gccatggata tagtgaggag
      541 aagcccagct ggtttagtaa taaaaattat tgctatagaa atatccttag aaatcattta
      601 tttgttactt tctacggcgc ttgtagaagt acgtagccaa gctggtgaaa gctatacact
      661 agcgcgagct attttatctg ttatgttctt aagtcttgcg ataactgctg tcgtcacctt
      721 agtcgctcag tgggcaaatg aaggctatta tattaaagaa aacgaattag ttgtcagacg
      781 gggtatcatt cacaaaacag aaatttctta cccctatgct aatatgcaat ctgtgtctgt
      841 tcaacaaggc ttttttggca gattatttaa ctttggttta gtaaccatct ttataccaac
      901 acttggtaaa gatatactat tcaacgaaat atcagcacct aaaaaatttg cacaaatatt
      961 gaaagaccac ataccgtacg ctgaaagcgg ccaattttta attcgcaaat agtttagcac
     1021 gaaagcagta acagcttaac gactgcttaa catcattgat gctaaactat aaatatgatg
     1081 aataaagtga atattctcgt cgtagaagat aatacagata tgcgaacagc tgtgtctgac
     1141 tatttgactg ataacggata tattgtccgg agtgccccaa gtgccgaact agcccaagaa
     1201 aagaactatc cagcggcctc tgatatagcg gttcttgaca tttcgttacc aggcataaca
     1261 ggtttatcat ttacaagaat gctcaaagaa caaggctttg atggcccgat aatagccata
     1321 acagcccgcg acacagtaga cgataaaatt ataggtttag aagctggtat ggatgattat
     1381 ttagtgaagc catttgactt acgggagcta gttgcccgaa taaacgcgca actacgtacc
     1441 aaaggtatcc aacacgatct tgctcctata aaaacggctc actttaaaat agagccaaaa
     1501 agacataaat tctttataca agacaaggag gtaaagctca cgcttgttga atttcgtatt
     1561 atgcgaaagt taatgcaaaa tagtcataca accgtgagta cgcaagatat tattgagtct
     1621 gcttggggcg atgacgctgc aataaccaac cccccaatac gcattcatat atctaacttg
     1681 cgcaataaaa ttaatgatcc tacgctaacg ataatccaaa ctatacctgg cgtcggctat
     1741 ttactaaatg attaataact atggataaag tacttgcaac agccagaaaa aatcttacat
     1801 ttgctacggt tggcgtagcg attatttgtg ctttggttgc atctattgct atgtatgcct
     1861 acgcagaaca gcgtttgcag caaaatattt ctgctgcaaa aattggccag cttgcacaag
     1921 acaattcaga actaagcgcc tacatagcca gccaagcacc aaatcatgcc cattttgaaa
     1981 ccattgctgg aatcatacaa agagaagaca gaaaccaaat taccgcgagt ttaccttttg
     2041 tgctcgtggc agtgattgtc atgagtgggc tggctggatg gtggctatcg cgccgcttgc
     2101 tcagccccgt aaaagaagcg tatctttcgc aacgacgttt tatgcaagat gctgctcacg
     2161 agttacgcaa tccgttagct gctatgagca caatgcttca gcaggcaaaa aatcggcccc
     2221 ctgcaggcaa agagctcaat acgttcattc aatctcttgg caggcaggca acacatctat
     2281 caagcataac aactgactta ctgctactag aacaccgtga ttacccgggt acacaagaga
     2341 tcgatatcgc cgaactatta ggagatattg tagaagagtt gcatcaccaa gcagtcgcca
     2401 agcatattac tgtgaatctt aaaaaaccaa caacgctcaa tacaaaaata gatcctcagc
     2461 attttgtgta catagcaaaa aatgtgctag aaaatgccat taaatttagt gacaaaaaag
     2521 gcaagccaat tgacgtacaa ctaacacatt caaaaacagg gtggacccta aaagtaagag
     2581 actatggtgt tggtatacca aagcaagatt tacccaatat tacacaacgt ttttacagag
     2641 caaaaaatgc gaccaataca gacgggaccg gcttaggcat ggcaataata gataaatttg
     2701 tttctatcta tcatggcgat gtcagcataa caagcaccgt cggtaaagga actaccgtaa
     2761 tagttacatt gtaactatac acattctata cacccctcca gtcataatag ttagtgtaat
     2821 taactaatta ggaggaacgt attatggaaa gtaacaaaac agcaggtatc gtagccgttg
     2881 tattagccat cgcagttgcc gttggttgta ttgtgtggtt cgcgaataga gaagatacta
     2941 cagctacaaa tgagacgagc caaagtaaca tgcaacctgc agaacaacag gccaaccgtg
     3001 acatagtggt ggtagcatca gaaacagaaa gcctcagcac cctagtgact gctgtaaaag
     3061 ctgcaaatct tgtagaaaca ttgcagggag caggaccgtt cactgtattt gccccaacca
     3121 atgatgcttt cgctgcattg ccagccggta cgctagacac gctgctcaag ccagagaacc
     3181 aagatcagct taaaagcatc ttgacctatc atgtcgtacc aggcaccgta ctcgctggtg
     3241 acctaacaga tggccaagtt atcactaccg tacaaggtgg cacacttact gttaaaatcg
     3301 ccgatggtat ggtctacctt gttgacgcaa caggcaacca agttaaggta gaaaaggctg
     3361 atgttaatgc agacaatggc gtcgtacacg tcattggcgg tgtactattg cctcagtaaa
     3421 tagcaggcgg catctttaac aaatattttg gttgtttctc agctaattat cagctagtaa
     3481 cgtaatacta atctgcgaca tagccgcata tatgcagtat gcacataagg taatccgcta
     3541 gaactattga ccctttatgt acatgtggtg tgtcttctgc gagggcacac cacataaaaa
     3601 ataataagca ttggaggtaa cgctatggac tttataagag acttacttgg gagtgtacaa
     3661 aacgtatctc agcaagcgat tccagagcca caagagctca tggatattgc actcggtagt
     3721 accagtatag acgaatctat tcaacaattt actgagaata ccgatacggt acaaacaatt
     3781 accgatagtc ttaagcgata gtgtcactct aggtgtagga gcgttgcgta tgcagcatta
     3841 cattcgctta acgtacgtca tatgaagcta ataattatca gtagtgtaag acgatgacat
     3901 gcaaatagta tgtaagacta gtaagtagaa tgcggctgat aattgcgaat acggcccggg
     3961 gatctccccc gggccgttcg ctagtttact gctttcgctc aacaaagagc catttggcac
     4021 cactctttcg ttgggcagga gtgggcaaga cagagacccg gaccacgttc ttgtagacga
     4081 cgtccactag cccagccagc ttggctctag tgagcttatc tgccttgagc gaagctctgg
     4141 cgacagactc ttttactacg atg
//
LOCUS       DAOPLS010000049         4161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_23534_length_4161_cov_4.115684, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000049 DAOPLS010000000
VERSION     DAOPLS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23534_length_4161_cov_4.115684"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PKV52_01745"
     CDS             complement(<1..771)
                     /locus_tag="PKV52_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPD98970.1"
                     /translation="MEFLDPEEKREKTLRLFMGYGFIAAVIIFSTFMISFILQGFDLF
                     NNNRDVRNGLLFIDSKPVSANIYINDVLEKRTDARFVLPENTYSVRLTEEGYRDWYKD
                     VDVIGGSVTYQIYPRLFPVDIRKQTTATYAQIPKIWTESPSRKWIVANANSTNPTLNV
                     TDTTKATEAPSQIVIPETVLSAQDMATSSLKVVEWSTDNRHFLVQKTASDGFISYVII
                     DREKPEESFDVTAKLSIAQGIDVRLRDKKYDQYYLLDAN"
     gene            925..2100
                     /locus_tag="PKV52_01750"
     CDS             925..2100
                     /locus_tag="PKV52_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98971.1"
                     /translation="MKTTIEINGKIYDAHTGKVVSSHPAKTDTSNAERKTPTRTQVTQ
                     PLQRGVIMDGVRKTPHHATNRPLSSAQPTPVKHVQKSQTLHRKAVQKPSTPKPVPHIH
                     STNANVHSSVEHSATGRGMLVKRVPDVRLSRALRASKSTLISKFGTPPNPKPHLTTEL
                     PVKEPPVHHQAPKSTHSSAGATTHQTKPVNTQPTTTSKETPTNPKKFVFEDHIKKANS
                     HLLKPVKKDSRRKRLVRSLKISPKVVSASAAVFALTLLAGFFAYQNVPMVSMRVAASK
                     AGFNGALPTNIPAGYSFNGPVQADKDVISLTYNSNSDARRFIVTQKPSNWSSEALLTN
                     YLLDSQFRYQAYKDNGLTIYIYNGSNATWVDKGIWYSVNGEDGSLSSEQLLELASSM"
     gene            2164..3606
                     /gene="gltX"
                     /locus_tag="PKV52_01755"
     CDS             2164..3606
                     /gene="gltX"
                     /locus_tag="PKV52_01755"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPD98972.1"
                     /translation="MHNITTRFAPSPTGFIHVGGVRTALFAWLLARQKGGTFILRIED
                     TDKQREVAGAEQHIIKSLQWLGIEWDEGPDKLGPHQPYRQSERLLLFADWAQRLVASG
                     RAYADPYTPAQVAAFREEAKRNKQPFLYRNHRPKNPPKWDGTQPLRFTSSPKAYAWHD
                     EVLGELSAGEDAVDDFILMKSDGYPTYNFCHIVDDSLMDVNLVIRSQEFIASVPKFLN
                     LYEALSVPIPTFATLPYVMAMNGNKKLSKRDGAKDILDYQNEGYLPEAMLNFLATLGW
                     NDGTEQEIFSKEELIKKFSLDKVQKSPARFDEKRLLWLNGQWIRRIDTDDLYNRVQPF
                     LPSSASSYSGTYKREVVALAQGRLKTLADIQAVAASFFEQPVPKPELVLAHKQLSKLS
                     PAVLSSLLAKTQSALQQQADFSIESIQDCLNSLLESTHQKPVILFSLIRLATTWAEYS
                     PDLAPSLALLGKDEVMQRLSKAEKYFDSIV"
     gene            3656..>4161
                     /locus_tag="PKV52_01760"
     CDS             3656..>4161
                     /locus_tag="PKV52_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HPD98973.1"
                     /translation="MDDIKTPKKDDSVPLSEPTLQSIAQKPTRLEDEIIEDEIIIDDS
                     KEPTNMPKSPKKSKNIFKKLTKKQLIILGVVCAIIVGFGIYYIFFTKDTTQPTTQNSI
                     TQEEPAPNQPPTSPLTGREVASAAIAARPITGIMIENSPESRPQSGLYQADMVIEAIA
                     EGGVTRFMA"
BASE COUNT         1338 a          897 c          838 g         1088 t
ORIGIN      
        1 gttcgcatct agtagataat attggtcata ctttttatca cgtaagcgta catcgatccc
       61 ttgtgcaata gatagctttg ccgtcacgtc aaatgactct tcaggttttt cgcgatctat
      121 tatgacatat gaaatgaaac cgtcggaggc cgtcttttgc accaaaaaat gacggttatc
      181 tgtagaccat tcaaccactt tcagtgaact tgtcgccata tcttgcgcgc taagaacagt
      241 ttctggtatg acgatttggc ttggcgcctc tgtcgctttt gtagtgtcag taacattaag
      301 cgtggggttc gtgctattgg cattcgcaac tatccatttt ctgctcggtg attctgtcca
      361 tatttttggt atttgtgcat acgtagcggt ggtttgttta cgaatatcta cggggaataa
      421 ccttgggtaa atttggtagg taacactgcc acctatgaca tctacatctt tataccaatc
      481 gcgatagcct tcttctgtaa gccgcactga ataggtattt tctggcaaga caaagcgggc
      541 gtctgtgcgt ttttctaata catcatttat gtaaatgttc gcactaaccg gtttactatc
      601 tataaataat aatccgtttc tgacatctct attattgtta aataaatcaa agccttgcaa
      661 aataaaagaa atcataaacg tactaaaaat aataaccgcg gctataaagc catagcccat
      721 aaataaacgt agggtttttt ctcttttttc ttcggggtct aaaaattcca tagtagtacc
      781 acaaattatt gtaacgcgta cacgttaaaa tcgctacggc atgttagcgg gtaagaacta
      841 acaaaaacac ttgcgtttat atactaaatg cttgtacgat agtagataag gcagaaattt
      901 aaaaaataaa ttttggtggc ataggtgaaa acaacgatag aaataaacgg caaaatatac
      961 gacgcacaca caggtaaagt agtatcatcg cacccagcaa agacagatac atctaacgct
     1021 gaacgtaaaa cgccgacacg cacacaagta acgcaaccat tacaacgtgg tgttattatg
     1081 gatggcgtgc gtaaaacgcc ccatcatgct acaaacaggc cgcttagtag cgcgcaaccc
     1141 acgccagtta aacatgttca aaaatctcag actctacatc gcaaagcagt acaaaaaccc
     1201 tctacaccaa agcctgtgcc acatatacac agcacgaatg caaatgtcca cagtagcgta
     1261 gagcacagcg ctactggtag aggtatgctt gttaagagag tccccgacgt gcgtctgagc
     1321 cgtgccttac gggcgagtaa aagtacgtta atatctaagt ttggcacgcc accgaaccca
     1381 aaaccacacc taacgacaga actccccgta aaagaacccc cagttcatca ccaagcccct
     1441 aaaagcactc attctagtgc tggtgctact acccatcaga ctaaacctgt taatacacaa
     1501 cccactacta cgagtaaaga aacgcctaca aatcctaaaa aatttgtttt tgaagatcac
     1561 attaaaaaag ctaatagtca cttgttaaaa ccagtaaaaa aagattcgcg tagaaaacgc
     1621 ctggtgcgat ctttaaaaat atcacccaag gtagtatcgg cgagcgcagc tgtgtttgcc
     1681 ttgaccctat tagctggctt ttttgcgtat cagaatgtac ctatggtaag tatgcgtgtc
     1741 gcagcaagta aagcaggctt taatggagca ttaccaacta acatacctgc agggtactca
     1801 tttaatggcc cggtgcaagc agataaagat gtaatttcac tcacttataa ctctaatagt
     1861 gacgcgcgac gctttattgt gacgcaaaaa ccatctaact ggtctagtga agcactactg
     1921 acaaattact tactagattc acaatttagg taccaagcgt ataaagacaa tggcttaacg
     1981 atttatatat ataatggttc taatgctaca tgggtagata aaggaatatg gtatagcgtt
     2041 aacggcgaag atggaagtct gtcatctgag cagctgcttg aactcgcatc cagcatgtag
     2101 taagttgttt ttcgcgttat aagttgagaa taactcgtgt atttgacata ctactaggcg
     2161 agtatgcata acattacgac acgatttgca ccgagtccaa ccggctttat ccatgttggt
     2221 ggcgtcagaa cagcactttt tgcgtggtta cttgcccggc aaaaaggcgg taccttcatc
     2281 ttgcgcatag aagataccga caaacagcgt gaagtagctg gggcagaaca gcatattata
     2341 aagagcttgc aatggctcgg catagaatgg gatgaggggc cagacaaact agggccacat
     2401 caaccatata gacaatctga acggctactg ctttttgcag actgggctca aagactggta
     2461 gcaagtggca gggcgtacgc ggacccctac acaccagctc aagtagccgc attcagagaa
     2521 gaagccaagc gaaacaagca accttttttg tataggaacc atcgcccaaa aaacccacca
     2581 aaatgggatg gtacacaacc gctcaggttt acatctagcc ctaaagccta cgcgtggcat
     2641 gatgaagtgc taggtgaact ttctgcaggc gaggatgctg ttgacgattt tattttaatg
     2701 aaaagtgatg gctatccgac atataatttt tgccatattg ttgatgattc gctcatggac
     2761 gtaaaccttg ttattcggag tcaagaattt attgcaagtg taccaaaatt ccttaatttg
     2821 tatgaagccc tgtctgtacc cattcctacg tttgcaacgt tgccatatgt tatggcgatg
     2881 aacgggaaca aaaaactatc taaacgtgat ggtgcaaaag atattttaga ttatcaaaat
     2941 gagggctatc tgccagaagc tatgctcaat tttttagcaa cgctcggctg gaatgacggc
     3001 acggaacaag aaatttttag caaagaagaa ctcataaaga aatttagtct agataaagtt
     3061 caaaaatcac cagcgaggtt cgatgaaaag cggttattgt ggctgaatgg tcaatggatt
     3121 cgccgaatag atacagatga tctgtataac cgtgtacaac catttttacc atcgtcagcg
     3181 tcgtcgtatt caggtactta taaacgtgaa gtagtcgcac ttgcacaagg aaggctaaaa
     3241 acacttgccg atatacaagc tgtcgctgca agcttttttg agcaaccagt gccaaaacca
     3301 gaactcgttt tagcgcataa acaactatca aaactatcgc cagctgtatt atcaagcctg
     3361 ttagccaaaa cccaatcagc ccttcagcaa caagcggatt tttctatcga atcaatacaa
     3421 gattgcctaa attcactact agaaagtact caccaaaaac ctgtcatatt atttagttta
     3481 attcgtttag cgaccacttg ggcggagtac agtcccgatt tagcaccttc actggcactc
     3541 cttggaaaag atgaagtcat gcagcgacta tctaaagctg aaaaatattt tgatagtatt
     3601 gtctaaaaag tggtacaatg accttaagaa gtaatgtcaa gggtacctat tacatatgga
     3661 tgacatcaaa acaccaaaaa aagacgattc agtgccacta agcgagccaa ccttgcagag
     3721 tattgcgcaa aagccaaccc ggctagaaga tgaaattatt gaagacgaaa ttattattga
     3781 cgactcaaaa gaacctacga atatgccaaa atcacctaaa aagagcaaaa atatttttaa
     3841 gaaattaact aaaaagcaat taattatcct tggtgttgtc tgtgcaataa ttgttgggtt
     3901 tggaatatac tatattttct ttacaaaaga cactactcaa ccgacaaccc agaatagcat
     3961 cacccaagaa gagcctgctc caaaccagcc acccacatcc cccctcactg gtagggaagt
     4021 agcgtcagct gcaattgcag ccaggccaat tactggtatt atgatagaaa atagcccaga
     4081 atcgcgtccg caaagtggct tataccaagc tgacatggtt atcgaagcga ttgcagaagg
     4141 tggtgtaacc aggttcatgg c
//
LOCUS       DAOPLS010000050         4157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_23570_length_4157_cov_3.379083, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000050 DAOPLS010000000
VERSION     DAOPLS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23570_length_4157_cov_3.379083"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1260
                     /locus_tag="PKV52_01765"
     CDS             46..1260
                     /locus_tag="PKV52_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPD98974.1"
                     /translation="MKQAISLYLHVHQPYRIRHYTIFDAGSDHQYFNDASPHALTNNR
                     FIVEKVAEKSYRPMNAVLKELLDTIEDFKFSLSIPGMLIDQLAEWAPDVLYSFQELVK
                     SGRVEIVAETYHHSLAFFYSRAEFEAQVLMHRQKVQEVFGVTPTAFRNTELSYNNDLA
                     YWADKAGYKAIITEGWDPILGWRSPNFVYRPAYTENIKLLLKNYKLSDDIAFRFGNQE
                     WEEWPLSAEKYVSWLGAAMDNAQVFNLFMDYETFGEHQWASTGIFDFMKAMPHIWKNS
                     RDDRTFMTISESVYSFEANDYVDIPQTITWADTERDLTAWLGNSMQHEAIHAIYQLED
                     KVLRCGDADIVADWRKLQTSDHFYYMCTKWFKDGDVHAYFSPYASPYEAFTNFMNAWH
                     DLQFRLAEKGFV"
     gene            1263..3197
                     /locus_tag="PKV52_01770"
     CDS             1263..3197
                     /locus_tag="PKV52_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HPD98975.1"
                     /translation="MARPVMLSNGQLMVGLNEAGLVHDFYYPYVGLDNLTTARSVHHK
                     IGVWVDGTFSWVDDDSWQVDIQLETDALIGRVELSHESLGIKISTKDFVDTQTNAFIR
                     RISVTNESNTRRDIRLFMHQVFEISKSGRADTAFYEPDGNYILDYKGRCALLICGQTK
                     DGHSLDQFAVGNYGIEGKAGTFIDAADGELSGNAVEHGGVDSVIRFSIPLDPHATAEV
                     DYWIIASSSQYESQAIHADFLRHGIDKREQIARNTWAKWLKQGDSALETIAPEYQDHV
                     KKSLLVIKAHIDKRGGILASGDSSIFNYGRDYYCYVWPRDGAYAIWPLIRMGYYKEAI
                     KFFEFCRDTMHKDGYLLHKYQPDRAIGSTWHPLVHNSRSELAIQEDETAIILIMLGEY
                     YDYSKDAEFTESLYATLIQPAAMFISKFIEEDTNLPHASYDLWEEKFLTTTYTTATVI
                     RALRRASSFADQFGYPDDAVQWMQVADSIESSLPLLYDPDRQAYRKGLLRLPDGNLQF
                     DNTLDMSTLYGLTMFTKVKLNDPTVVHTVEAIESNLVNKTIGGVVRYENDNYMKTSED
                     AQPNPWFVCTLWLAQYYVQRQQPDKAKELIDWSLSHSLDSGILSEQVHPETGYAVGVA
                     PLVWSHTELINTILDISGTQ"
     gene            complement(3249..3455)
                     /gene="rpmG"
                     /locus_tag="PKV52_01775"
     CDS             complement(3249..3455)
                     /gene="rpmG"
                     /locus_tag="PKV52_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPD98976.1"
                     /translation="MAKKSTKRKTIGLVCEETGHRHYYTRKNTQNSPDKLRLRKYNPV
                     VRRHTVYVETTKNLGRNVVKPRKK"
     gene            complement(3531..3716)
                     /locus_tag="PKV52_01780"
     CDS             complement(3531..3716)
                     /locus_tag="PKV52_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPD98977.1"
                     /translation="MKKLYRSRTNSVLFGICGGIAEYFNIDATVVRVATVVLGVLSGG
                     TIVLAYILCLFIMPKAQ"
     gene            complement(3722..3796)
                     /locus_tag="PKV52_01785"
     tRNA            complement(3722..3796)
                     /locus_tag="PKV52_01785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3762..3764),aa:Thr,seq:ggt)
     gene            complement(3776..3949)
                     /locus_tag="PKV52_01790"
     CDS             complement(3776..3949)
                     /locus_tag="PKV52_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98978.1"
                     /translation="MSNYDPHRFAETQSTHGLVYNPLGNIDADYLLDNQHNLGLLQEM
                     MGSTAYDAVVAQG"
     gene            complement(3955..4040)
                     /locus_tag="PKV52_01795"
     tRNA            complement(3955..4040)
                     /locus_tag="PKV52_01795"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4004..4006),aa:Tyr,seq:gta)
BASE COUNT         1248 a          842 c          914 g         1153 t
ORIGIN      
        1 tgtaggcgca tatacgcagc acgttcaact tgtgggggct aagtaatgaa gcaagctatt
       61 agcctttact tacacgttca tcagccctac cgtattagac attacactat atttgatgcc
      121 ggaagcgacc atcaatactt taatgacgca agcccgcacg cactaaccaa caaccggttt
      181 attgttgaaa aagtggccga aaaatcatac cgaccaatga acgcggtgct caaagaatta
      241 ttagacacaa tagaagactt taaatttagt ttgtctatac ctggtatgct cattgatcaa
      301 ctggccgagt gggctccaga cgtactatat tctttccagg aactcgtaaa aagtggccga
      361 gtagaaattg tggcagaaac ctaccaccac agtttggcct tcttttattc gcgtgcagaa
      421 tttgaagccc aagtacttat gcaccgccaa aaagtccaag aagtgtttgg tgtaacccct
      481 acggcattcc gaaacaccga acttagttac aataacgatt tagcttattg ggcagataaa
      541 gcgggttata aagccataat tacagaaggt tgggacccta ttttaggatg gcgtagccct
      601 aattttgtgt accgtccagc ttacacagaa aatattaaat tactacttaa gaattataag
      661 ctaagtgacg atattgcgtt tcggtttggt aaccaagaat gggaagaatg gcccctcagt
      721 gccgaaaagt atgtatcgtg gcttggtgca gcgatggata atgcccaagt atttaatttg
      781 tttatggatt atgaaacgtt tggcgagcac caatgggcga gcacaggtat atttgatttt
      841 atgaaagcca tgccacatat atggaaaaat agccgtgatg atcgcacgtt tatgactatt
      901 agcgaatctg tctatagctt tgaagccaac gattatgttg atataccgca aacaattaca
      961 tgggccgaca ctgaacgaga tttaactgca tggcttggta atagtatgca gcacgaagca
     1021 atacatgcca tatatcagct cgaagataaa gtactacgtt gtggtgacgc cgatatcgtg
     1081 gcagactggc gtaaactaca aaccagtgat catttttact atatgtgtac caaatggttt
     1141 aaagacggtg atgtacacgc atattttagc ccttatgcca gcccgtacga agcgtttacg
     1201 aactttatga atgcgtggca cgacttgcag ttccgccttg cagagaaagg gtttgtttaa
     1261 gcgtggctag accagtcatg ctgagtaatg gccagctaat ggttggcttg aatgaagccg
     1321 gcttagtcca tgatttttat tacccatatg tggggttaga taatcttaca accgccagaa
     1381 gtgtgcacca taaaattggt gtatgggtag atggtacgtt ttcttgggta gatgatgatt
     1441 cctggcaggt agatattcag ctagaaacag atgcgcttat tggtagagta gaactatcac
     1501 atgaaagtct tggcataaaa atcagcacaa aagattttgt agacacgcaa acaaacgcct
     1561 ttatacgacg catatcagta accaatgaaa gtaatactcg cagagatatt cgtctgttta
     1621 tgcatcaggt ttttgaaata tctaaatctg gccgtgccga cacagctttt tatgaacctg
     1681 acggtaatta cattttagat tataaaggcc gttgtgcctt gcttatatgt gggcaaacaa
     1741 aagacggtca ctctctagac caatttgctg ttggtaatta cggcatagaa ggcaaagctg
     1801 gtacttttat tgatgcagct gatggtgaat tgtctggcaa cgccgtagaa catggtggcg
     1861 tagattctgt catacggttt tctataccgc tagatccaca tgcaacggct gaagttgact
     1921 attggattat tgcgagtagc tcacagtatg agtcacaagc tatccacgca gattttttgc
     1981 gacacggtat agataagcgt gaacagattg cccgaaatac atgggcaaag tggttaaaac
     2041 agggtgacag cgcactagaa acaattgcgc ctgagtatca agatcatgta aaaaaatcat
     2101 tgcttgtcat taaagcacat atagacaaac gaggtggtat attagcgagt ggtgatagta
     2161 gcatatttaa ttatggccga gattactact gttatgtatg gccccgcgac ggggcatatg
     2221 ctatatggcc acttataaga atgggttatt acaaagaagc gattaaattc tttgaattct
     2281 gtcgtgatac gatgcataaa gacggctatt tgctccataa gtatcagcca gatagagcaa
     2341 ttgggagtac ctggcaccca ctggtgcaca atagtcgttc ggagctggca atacaagaag
     2401 acgaaactgc aataatcctt ataatgttgg gtgaatatta tgattatagt aaggatgccg
     2461 aatttaccga atcgctgtat gccacattaa tccagccggc tgcaatgttt attagtaaat
     2521 tcatagaaga agacacaaac ttgccgcatg cgagctacga tttatgggaa gagaaatttt
     2581 taacaacaac ctataccact gctactgtta tacgcgcatt acggcgagca agtagctttg
     2641 ctgaccagtt tggataccca gacgacgccg ttcagtggat gcaggttgca gatagtatag
     2701 aatcatcact ccccttgctg tacgaccctg ataggcaagc gtatcgtaaa ggacttctgc
     2761 gcctgccaga tggtaatcta cagtttgata acacgttgga tatgtccacg ttatacggac
     2821 ttaccatgtt tacaaaagta aaattaaacg atccaacagt tgttcacacg gtagaagcta
     2881 tagaaagtaa cctcgttaac aaaactatcg gtggagtcgt gcgatacgaa aacgataact
     2941 acatgaaaac atcagaagat gcgcaaccga atccatggtt tgtgtgcaca ttatggcttg
     3001 cgcagtatta tgtacagagg caacagccag ataaagccaa agaactcata gattggtcac
     3061 tgtcacatag cctagatagt ggcatactaa gtgaacaagt acacccagaa accggctatg
     3121 cggtgggcgt tgctccgtta gtatggagcc acacagaact tattaatacg attcttgata
     3181 ttagcggaac acagtaaata tccgctacac gataacgtag cggatgctat aactgctacg
     3241 actttaggct atttctttcg tggtttgact acgttacggc ctaggttctt ggtcgtttct
     3301 acatagacgg tgtgacgccg tactacagga ttgtacttgc gcagacgcag tttgtctggg
     3361 ctattttgag tgttcttacg ggtatagtaa tgccgatgtc ccgtttcttc acacactaat
     3421 ccaattgttt tacgcttggt tgattttttt gccataatta attagcctct acatactttc
     3481 aacttattat tttactgcaa atacccttta aaagcaatag ttggttcggc tcattgcgct
     3541 tttggcataa tgaataaaca taatatatat gcaagtacaa tggtaccacc agataataca
     3601 ccaagtacga cagtagccac tctaacgaca gtagcatcta tattaaagta ttcggctatg
     3661 cctccacata taccaaacaa tactgaattt gtgcgtgaac gatataactt tttcatattt
     3721 atggagccgt tgtcgggatt tgaacccgag accccttcct taccatggaa gtgctctacc
     3781 cctgagctac aacggcatca tacgctgtgc tgcccatcat ttcttgcagc aaccctaggt
     3841 tgtgctgatt atctaataga taatcagcat caatattgcc aaggggattg tacactaagc
     3901 catgtgttga ctgagtctct gcaaaacgat gtggatcata attactcata tatgtggtgc
     3961 agggtgtagg attcgaacct acgaaggcat aagccaacag atttacagtc tgtcgtgttt
     4021 gaccgcttca ctaaccctgc atgtaaaaaa atgatattaa aagattgcgt atcggcatga
     4081 tactggagcc ggtggaggga ttcgaaccca cgaccctctg tttacaaaac agatgctcta
     4141 acccctgagc tacaccg
//
LOCUS       DAOPLS010000051         4093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_24057_length_4093_cov_4.079495, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000051 DAOPLS010000000
VERSION     DAOPLS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24057_length_4093_cov_4.079495"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKV52_01800"
     CDS             <1..250
                     /locus_tag="PKV52_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98979.1"
                     /translation="MIPKLSGGEVLERMRASEWGKNIRVYVISNLNEREAPANLRSLG
                     IEGYAVKANLTDDDIDKLVDSILMPPEQKAEESSNVLE"
     gene            341..772
                     /locus_tag="PKV52_01805"
     CDS             341..772
                     /locus_tag="PKV52_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HPD98980.1"
                     /translation="MWFAQETITPVMLSPETTVTTTDSTSFWGAIAALWILYVVVIVV
                     AIVAQWKIFTKAGEAGWKSIIPFYNMYTLFTIAGRNGWWFLAFLVPIVNIFVALMLAI
                     DLAKHFGKSTAFGVVALWLFSIVGYLILGFGDAKYVGPKHD"
     gene            complement(801..1253)
                     /locus_tag="PKV52_01810"
     CDS             complement(801..1253)
                     /locus_tag="PKV52_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98981.1"
                     /translation="MYRFKKNYKNYLRYNKHIRACALCAPEDFTIIEQTEYAVVVVNN
                     YPYDIWEQSVVLEHLMVLPLEHKANLSELSPAIQNDIMALLAAYETMGYNIYARSANS
                     KHRSVAHHQHTHLIKITNDYARASLYIEKPYLLKIFGRHAKSKKTAQN"
     gene            complement(1321..3063)
                     /locus_tag="PKV52_01815"
     CDS             complement(1321..3063)
                     /locus_tag="PKV52_01815"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPD98982.1"
                     /translation="MKLHKIVPQLIVFLVLIIFVAPSTAHANTQNFTIASFDATYTLG
                     KDDNNRSILSVEETITAQFPNYDQNHGIERAIPNTYDGHSVSLSIESVNKPDGTNWSY
                     TTYQSNGNTVLRIGDANRYLQGEQIFIINYMMRDVTKNFSGGDEFFWDINGTDWQQTI
                     ERVQATVYLEDSIKAAFDNRLQCYSGVAGSTASDCTISASIDGVITATSTQPLAAQEN
                     LSIVVGFAPNTFSGYVAPPIPLWLKLFWFVVVPIGYVIFPILVLLWAVRTWRSKGRDV
                     TKQTTIVPQYIPPKNTSMLLNDTIINTKMRPKAVSASIIDLAVRHYLKIYEVKKKEYE
                     LEINKPVDGAHPDDVSVLQLLFGNSLQVGSRIALKDKKTIYKQVEQLGKDTYQAAIAS
                     GYMADTRAVQKKVYIVGGIFTFISIVTLNPLLFVAAITTLVLGAYMPARTAKGIELKS
                     YLDGTKMYMQVAEAERLKVLQSADSAIKVNTRSADQLVKLYERLLPLAMLYGIEKSWA
                     TQFAHLYTPETQPDWYVGNYSTFNAAVFAGSLASFGSSSVSSFSPPSNSSSSGFSGGG
                     SSGGGGGGGGGGGW"
     gene            3153..3818
                     /locus_tag="PKV52_01820"
     CDS             3153..3818
                     /locus_tag="PKV52_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HPD98983.1"
                     /translation="MRGKLFDNTENTTNENKTFVAQNDFTLLVNLDGTIYAKQGSMIA
                     YKGTVDFDYKGAGIGRFVKSVATGEGMSLMKVAGQGDVFLADRAAQIHVLDLENEALT
                     ITSRNVLAFTEGIDWDINVLKAGVMGFAAGGFFNTTLKGTGSISITSFGLPVVIAVDG
                     NEVYADVNSIIAWTSDLQVSIKSSFKAKSLIGRGSGESFQMAFRGKGHIVVQPGEYLT
                     ARA"
BASE COUNT         1160 a          802 c          872 g         1259 t
ORIGIN      
        1 gatgattcct aaactatctg gtggtgaagt actcgagcgt atgcgggcat ctgagtgggg
       61 taaaaatatt cgtgtatatg tgatatctaa cctgaacgag cgtgaagctc ctgctaattt
      121 acgtagtttg ggtatagaag gttacgccgt caaagctaat cttacagatg acgacataga
      181 taaactggtt gatagcatat taatgccacc agaacaaaaa gcagaagaat ctagcaacgt
      241 tttagaataa ttacttgttg atataatgcc tttttttgca atattgttaa tattacaaaa
      301 gtacgtatac tggtgatata acttaaggag gattatagtt atgtggtttg ctcaagaaac
      361 aataacacca gtaatgctgt cgcctgaaac tacggtgact acaactgata gcacgagctt
      421 ttggggtgca atagctgcac tttggatact atacgtcgtt gtcattgtgg tagctatcgt
      481 tgcacaatgg aagattttta ctaaagccgg cgaagctggg tggaagtcaa tcatcccttt
      541 ttataatatg tatacattat ttaccattgc tggccgcaac ggatggtggt ttttggcgtt
      601 cttggtgccg attgtgaata tttttgtagc tttaatgctg gctatcgatt tagcaaaaca
      661 ctttggaaaa agtactgctt ttggtgtcgt ggcgctatgg ctattttcaa tcgtaggcta
      721 tttgatacta ggatttggtg acgcaaaata cgttggtcca aagcacgact aaaatctggt
      781 ttgttctata aaaaaaccgt tcagttctgg gcggtttttt tagattttgc gtgcctaccg
      841 aatattttta aaagatatgg cttttcgata tatagtgatg ctcttgcgta atcgttggta
      901 atttttatta aatgggtgtg ttgatgatgc gccactgaac gatgcttgct attggctgat
      961 cgtgcgtata tgttataacc catagtttca tatgctgcaa gcaacgccat gatgtcattt
     1021 tgtatggctg gtgacagctc actcaaatta gctttatgtt cgagtggcag taccattaag
     1081 tgttccaata cgactgattg ttcccaaata tcatatgggt aattattcac gacaacaacg
     1141 gcgtactctg tttgttcaat aattgtaaaa tcttcaggtg cacaaagagc gcacgccctt
     1201 atatgtttgt tgtaacgtag gtaattttta taattttttt taaatctata cactaataca
     1261 atactaacac tcgaggtggg cttatgctag atagattgtg atcgccgcgc gcgtgcagta
     1321 ctaccaacca ccaccgccac caccgccacc accgccaccc gaactgcctc cacctgaaaa
     1381 cccactactg ctactattgc taggtgggct aaagctcgat acgctgctac taccaaaact
     1441 ggcaagtgaa cctgcaaaaa cagccgcatt aaaggtgctg taattaccaa cgtaccagtc
     1501 tggctgtgtt tctggtgtgt ataaatgagc aaattgcgtg gcccaacttt tttcaatgcc
     1561 atacagcata gcgagtggga gcaagcgttc atataattta acgagctgat cagcactgcg
     1621 tgtattcact tttatagcag aatcggcaga ctgtagaacc tttaaacgct ctgcttcggc
     1681 tacttgcata tacattttag tcccatctag gtagcttttt agttcgatgc cttttgcagt
     1741 acgtgctggc atatacgcac cgagcacaag cgtagttatt gctgcaacaa agagcagtgg
     1801 attaagggtt acaatactta taaatgtgaa gataccgcca actatataaa cctttttctg
     1861 gacagctctt gtatcagcca tgtacccact agcaatcgca gcttgatacg tatctttacc
     1921 tagctgttca acttgcttgt aaatagtttt tttgtctttt aaggcgattc tgctacctac
     1981 ttgcaacgaa ttaccaaaca atagctgcag cacagataca tcgtcagggt gtgcaccgtc
     2041 tacaggtttg tttatctcta attcgtattc ttttttctta acttcgtata ttttaaggta
     2101 gtggcgtacc gcaagatcga taatgctcgc agatactgct ttgggccgca tttttgtgtt
     2161 gatgatggta tcgtttagca acattgatgt gtttttgggc ggtatatact gcggcactat
     2221 ggtagtttgt tttgttacat cacggccttt tgagcgccaa gtccgcaccg cccaaagcaa
     2281 tactaagata ggaaatatta catagccaat tggcacaact acaaaccaaa agagcttgag
     2341 ccacagcggt ataggcggtg cgacgtaacc gctaaaagta tttggtgcaa agcctacaac
     2401 aatacttagg ttttcttgag ccgctagagg ttgcgtagac gtggctgtta ttaccccatc
     2461 tatagaggca cttattgtac agtctgacgc tgtggatcca gctactccac tgtagcactg
     2521 taacctatta tcaaaggctg ccttaatgga atcttctaaa tataccgttg cttgtactcg
     2581 ctcaatagtt tgctgccaat ctgtcccatt gatatcccag aaaaattcat caccaccaga
     2641 aaaattctta gtaacatcgc gcatcatgta attaatgatg aatatttgtt caccttgcag
     2701 atatctattg gcatcaccaa tgcgaagcac cgtattacca ttactctggt aggttgtata
     2761 cgaccaatta gtcccatctg gcttgttaac actctctata gataaactta ccgaatgacc
     2821 atcatatgtg tttgggatag cccgctctat accatggttt tgatcataat taggaaattg
     2881 tgcggtaatg gtttcttcta cacttagaat agacctgttg ttatcgtctt tgccaagcgt
     2941 gtatgttgcg tcaaaggacg caattgtaaa gttttgtgta ttggcatgtg cagttgatgg
     3001 cgctacaaaa ataatgagaa caagaaacac aataagttga ggtactattt tgtgtagctt
     3061 catgccttta ttgtagcaaa ctgcctaaat atgtagtggg cagtattgct ttgctatact
     3121 taggacagat taatactaga aagggcttac ttatgcgcgg caagctattt gataatacag
     3181 aaaacactac gaatgaaaac aaaacgtttg tagctcaaaa cgacttcacg ttgctcgtta
     3241 atttagacgg cactatttat gcaaaacaag gcagcatgat tgcctataag ggcaccgttg
     3301 attttgatta taaaggtgca ggaattggtc gttttgtaaa aagtgttgct acaggagaag
     3361 gcatgtctct tatgaaagtt gctgggcaag gagatgtatt tttagcagac agagctgctc
     3421 agatacatgt attagactta gaaaacgaag cactaacaat cacttctcga aatgtgcttg
     3481 catttacaga aggtatagac tgggatatta acgtactcaa agcaggtgtt atgggttttg
     3541 cagctggtgg tttttttaac acgactctaa aaggtactgg ctccatttca attacaagct
     3601 ttggtttgcc agtggttata gcggtagacg gcaatgaggt ctacgcggac gtcaatagta
     3661 ttattgcgtg gacaagtgac ttgcaagtat ctataaagag cagctttaag gcgaagtcac
     3721 ttattggccg cggaagtggt gaaagctttc agatggcctt tcggggcaaa gggcatattg
     3781 tcgttcaacc tggtgaatac ttaacggctc gcgcttaggc tactgactaa ttttacttag
     3841 tataaggaat attaaaacta ttaatcccag gcttcttctg aaataattta ctccactcac
     3901 aagttctaaa aatgcccagc tagcaagagc aacagtacct atggtactta aaactgtatc
     3961 tgccgtagaa ctagtactta tgctagtcgc cacaactgcg ccgaaccata caagaattgg
     4021 aatattcgga aactgggcaa tgactatgtg cccattttta tcagtaaata gcttacgtaa
     4081 tgcgctcacc tat
//
LOCUS       DAOPLS010000052         3933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_25409_length_3933_cov_4.092058, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000052 DAOPLS010000000
VERSION     DAOPLS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25409_length_3933_cov_4.092058"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..915)
                     /locus_tag="PKV52_01825"
     CDS             complement(40..915)
                     /locus_tag="PKV52_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPD98984.1"
                     /translation="MDHRFKKYLAVADTGSFSAAAKKLKVTQPAITLAVASLERAFGT
                     KLCVRNKHTITLTPDGEVVARSAKKIAVEIEKMEATLTNAQDKSQYNVGIIDSIAHLL
                     YNSTDALLKNKFEVMVDNSRRIVNDVLASKIDIGVITGQPTTIGNSVTTHKLHNEEFV
                     FVCAPYMAPNKAVTSIDDWLAINQDSTSYRHFVKLFKKEGLRVTPVFYSTSMELLKEM
                     AVAGKGTALLPRHLVSESIDNGTLDIVATKPLYRPIWAVTKKEQNAPVIIQLTRQIDD
                     LLASNIYKSTSMHAR"
     gene            1018..2133
                     /locus_tag="PKV52_01830"
     CDS             1018..2133
                     /locus_tag="PKV52_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPD98985.1"
                     /translation="MSKIKIAVVGAGNCFSSLYQGLEYYKDHDDSSIPGIMWATIGGY
                     HPRDIEVVAVYDVDKRKVGKPTGAAIFAKPNCARVFQPNVPSGPMVEMGTVLDGVSEH
                     MLTQPEDQGFRLSKEKPVDIVASLKKSKADILVNYLPVGSQAATEFYAQAAIDAQVAF
                     LNCIPVFIASDPTWEQKFIDAGLPIIGDDMRSQLGASVLSQVLQELAFDRGLIVDFHQ
                     QLNIGGNTDFNNMMVQSRLASKKKSKENVIRAQNDLRNIPVAEHSLFAGPSTYLPYLE
                     DNKVAYFNLRLRGFGDAPVTIDVKLSVQDSENSAGVVIDAIRYLKVARELGVVGALRG
                     PSAWTQKTPPQQMMYADAKNECEALAHRKLTNSVKRK"
     gene            2206..3537
                     /locus_tag="PKV52_01835"
     CDS             2206..3537
                     /locus_tag="PKV52_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPD98986.1"
                     /translation="MEQESLNAARVLMGDSLGFHIVFVMFGIGLPVVFSLLEFIGIKY
                     KKPRLIDAAKFISYIATVLVITGVVSGTLIAIQMSLMWPKLPEFGSAIIGLPFMFEGY
                     AFVLEALFLGYYMYTWDKIKGYRHWLLSLPIILGAFLSAFFITSVNSWMNKPGGFDIV
                     DGKIVNADVWAGILTPTTFFMVSHSVLGYYLAIFLAVIAGYAWYIMRHKPKGASKKTA
                     EYIISRFALISFAVVLSIGAIGHFQTQYLATSQPRKLAAIELVPQTTTNAPYIIGGEL
                     SADGQSVEGGLRIPNGLSILAGNSPNTEIKGLNEFPKEDWPNLIVNTFFEIKMALVGF
                     LIAVPLLFLMLYRFRRNKAFSKPLLIGFIITAPIAFVVVELGWMVTEFGRQPYVVNGY
                     LRTSEAFVNNPGVIQWGYIFPILFVLLLVVTVLAVRQVIKKYSYLVTGDKK"
     gene            3534..>3933
                     /locus_tag="PKV52_01840"
     CDS             3534..>3933
                     /locus_tag="PKV52_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98987.1"
                     /translation="MIAYAFVLLPILAYLPFFFIESWAAFQRIGKKNPTSNEYLHITW
                     EMTHTLLILGVNYFIWLYASIVVQVGQAVYWGLIIAGALFIIRAVLYLYIFYSRPSSP
                     RLHNGLADWLFAISHVLIIGCLLYVVIRAGI"
BASE COUNT         1112 a          741 c          820 g         1260 t
ORIGIN      
        1 aacgatactg ctgcggcgac tcgttagcta ggcaagactt tagcgtgcgt gcatagacgt
       61 actcttgtag atgttacttg caagcaaatc atctatttgg cgagtaagtt gtataattac
      121 aggcgcgttc tgttcttttt tagtcactgc ccagataggc cgatatagcg gttttgtcgc
      181 aacaatgtct aatgtgccgt tgtctataga ctcacttacg aggtggcgtg gcaaaagagc
      241 tgtccccttg ccggccaccg ccatttcttt tagtagttcc atactcgtag aatagaacac
      301 tggtgtcacc cgtaaacctt cttttttaaa taattttaca aaatgtctat agctcgtaga
      361 gtcttggttg attgccaacc agtcatcaat agaggttact gctttatttg gcgccatata
      421 tggtgcacaa acaaaaacaa attcttcatt atgtagctta tgtgtcgtta ctgaattacc
      481 tatggtagtt ggctgaccag ttattactcc aatgtctatt ttactcgcta atacgtcgtt
      541 aacaattcgg cgtgaattgt ctaccattac ttcaaattta ttttttagca atgcatcggt
      601 agagttatat aaaagatgtg caatgctatc tataatgcct acattgtact gacttttatc
      661 ttgcgcatta gtaagcgtag cttccatttt ttctatttct acagcaattt tttttgctga
      721 gcgtgctact acttcaccat ctggagttaa agtaattgta tgtttatttc ttacacatag
      781 cttggtgcca aaagcacgct ctaaagaagc taccgcaagc gttatagctg gttgggtaac
      841 tttaagtttt tttgcagctg cagaaaaact acctgtatct gctaccgcta aatacttttt
      901 aaacctatgg tccattaatc tctattatac agtattaggt tttcttattt ttattattag
      961 tttgttttat atgaattagt gaattattat gtatatatcg taacaattgg ggggtgtatg
     1021 tctaaaatta aaattgcagt cgttggagca ggtaactgtt tttcatcgct atatcagggt
     1081 cttgaatatt acaaagacca tgatgatagt tctattcctg gcattatgtg ggcaactatt
     1141 gggggatatc atcctagaga catagaagtg gtggctgtat acgatgtaga taaaagaaaa
     1201 gtaggtaaac ctactggtgc ggctatcttt gcaaaaccaa attgcgcacg ggtattccaa
     1261 cctaatgtac caagtgggcc gatggtagaa atgggtaccg tattagatgg tgtgtcagag
     1321 cacatgctca cgcaaccaga agatcaaggt tttcgcttat ctaaagaaaa gccagttgat
     1381 attgtagctt cacttaaaaa atccaaggca gatatactgg ttaattattt acctgtagga
     1441 tcacaggctg caactgaatt ttatgcgcaa gctgcaatag atgcacaggt tgccttttta
     1501 aactgcattc ctgtgtttat tgccagtgac ccaacatggg agcaaaaatt tattgacgct
     1561 gggttaccta taataggcga tgacatgcgc tcacaacttg gtgcaagtgt gttaagccaa
     1621 gttctccaag aactggcgtt cgatagaggt cttattgtag attttcacca acaactgaat
     1681 attggtggca acaccgactt taataatatg atggtgcaat cccgccttgc cagtaagaaa
     1741 aaaagtaaag aaaacgttat tagagcccaa aacgatttac gcaatatacc cgtagcagag
     1801 cacagtttat ttgcaggtcc tagcacttat ttaccgtatc tagaagataa caaggttgct
     1861 tattttaatt tgcgtttacg cggttttgga gatgcaccag tcactataga tgtaaaactt
     1921 tccgttcaag atagcgaaaa cagtgctggt gttgtaatag acgctatacg gtatttaaaa
     1981 gtcgctcgtg aattaggtgt tgtcggtgct ttgcgtgggc cgagtgcttg gacacaaaaa
     2041 acaccaccac aacaaatgat gtacgccgat gctaagaatg agtgcgaagc tctcgctcat
     2101 cgcaaactca ctaattctgt taaacgtaaa taagtaacag gtaaataagc attagcaata
     2161 tgctaatttt gtcgttataa ttgggtacag taagtatata aaaaaatgga acaagaaagc
     2221 cttaatgcag cacgagtact gatgggtgat agccttggct ttcacatagt gtttgtgatg
     2281 tttggtatcg gtttgcctgt tgttttttct ctactagaat tcattggtat taaatataaa
     2341 aaacctcggc taatagatgc ggctaagttt atatcctaca tagccactgt gcttgttatt
     2401 actggtgtag tatctggaac attaattgct attcagatgt cactcatgtg gcctaaacta
     2461 ccagagtttg gtagcgccat tattgggcta ccgtttatgt ttgaaggtta tgcgtttgtg
     2521 ctagaagcct tattcttagg ttattacatg tatacatggg ataagattaa ggggtaccga
     2581 cattggctgc ttagtcttcc tataatactt ggcgcatttt tatctgcgtt ttttattacc
     2641 agcgtaaact catggatgaa caagccaggg ggatttgata ttgtagatgg caaaatagtc
     2701 aatgctgacg tatgggctgg aattttaaca ccaacaacat tctttatggt gagccattcg
     2761 gtgctaggat attatttagc tatattttta gctgtcattg caggctacgc atggtacata
     2821 atgcgccaca aacccaaagg ggctagtaaa aaaactgcag aatatattat aagtagattt
     2881 gctctgatta gctttgcagt cgtactgagt attggcgcca taggacactt tcaaacacaa
     2941 tacctcgcta cgtcccagcc cagaaaactc gctgccatag aattagtgcc gcaaacaact
     3001 acaaacgcac cgtatattat tggtggagaa ctgagcgccg atggtcaaag tgtcgaaggt
     3061 gggttacgta taccaaatgg cctcagtatt ctagctggca acagccctaa cacagaaatt
     3121 aagggattaa atgaatttcc taaagaagat tggccaaatt taattgttaa tacgtttttt
     3181 gaaataaaga tggccttagt aggttttctt attgctgtac cgctactatt tttaatgctg
     3241 taccgcttcc gcagaaacaa ggctttttca aaaccactcc tcattggatt tattataacc
     3301 gcccctatag catttgttgt tgtagaactc ggctggatgg tcaccgaatt tggcaggcaa
     3361 ccttacgttg tcaatggcta cctaagaacg tccgaagcat tcgtcaataa tcctggcgtc
     3421 attcagtggg gttacatttt ccctatatta tttgtactgc tcctggtagt taccgtgctt
     3481 gctgtacgac aggttatcaa aaaatatagt tatttagtga ctggtgataa aaagtgatag
     3541 cctacgcgtt tgtattatta ccaattctag cgtatcttcc ctttttcttt attgaatcat
     3601 gggcggcgtt tcaaagaatc ggcaaaaaaa acccaaccag caacgagtat ctacatataa
     3661 catgggaaat gacgcacacc ttattgatac ttggtgttaa ttattttata tggctttacg
     3721 caagcattgt cgtacaggta ggccaagccg tgtactgggg tttaatcatt gctggagctc
     3781 tgtttataat aagagccgtc ctgtatctgt atattttcta ttcccgacct tcgagccctc
     3841 gtttacacaa tggactcgct gactggttgt ttgcaattag tcacgtactc attattggtt
     3901 gtttgttata cgtggtcata cgggcaggca ttg
//
LOCUS       DAOPLS010000053         3793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_26728_length_3793_cov_3.430979, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000053 DAOPLS010000000
VERSION     DAOPLS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26728_length_3793_cov_3.430979"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..565)
                     /locus_tag="PKV52_01845"
     CDS             complement(182..565)
                     /locus_tag="PKV52_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPD98988.1"
                     /translation="MSSIDQQFVEYIVKSLVGNPDAVEVTRTIDEKGVLLELTVAPED
                     LGRVIGKRGSTAQSIRSLLRALGTKNDARYNLKIVDPSGGVRPATSDNTDDKDETTDS
                     EDSSSHSSVVSKTRQELADLDDLDV"
     gene            complement(660..1049)
                     /gene="rpsP"
                     /locus_tag="PKV52_01850"
     CDS             complement(660..1049)
                     /gene="rpsP"
                     /locus_tag="PKV52_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPD98989.1"
                     /translation="MLAIRMQRTGRKKMPHYRVVVQDSRRTPTSGKVVAKLGSYNPHT
                     KELTLDKDLSQTYLNNGAQPSERVVSIFKKEGVVIPSWVKERTVKNRKVRKADKLRAN
                     QPAPEQETVAEEVAKDLPEEAPAPADA"
     gene            complement(1137..1856)
                     /gene="rnc"
                     /locus_tag="PKV52_01855"
     CDS             complement(1137..1856)
                     /gene="rnc"
                     /locus_tag="PKV52_01855"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPD98990.1"
                     /translation="MQDYSNYQTLASQVLGGKFSDVMLLVTAFTHRSYLNEHKKTVKE
                     HNERLEFLGDAVLELVVTEYLYRNYTEPEGILTNWRSALVRTESISAAGERLGFNDYL
                     RLSRGEKRGSDRARQQILANCYEAVIGAIYLDQGYDAASTFISNSLLTTFETILATGS
                     WMDPKSRLQEIAQSVDNATPQYKVLSEEGPDHEKLFTVGVFVDSQLKGQGAGPSKQAA
                     QQQAAELALASYTNKTPEPKS"
     gene            complement(1843..2346)
                     /locus_tag="PKV52_01860"
     CDS             complement(1843..2346)
                     /locus_tag="PKV52_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD98991.1"
                     /translation="MAINRPKPIEGFKKYFHRKRPSITEVVKEATAGGIVWRRNKKNK
                     IEILLHKDPKERWTIPKGHIEPGETARETAEREIREETGLQEMKVMNWLGKINFQYRR
                     QNSLILMTTHIYLVQAKGDTNNIRKEDFMKSIAWLDANDALEKIEYEDIGKLILLGLK
                     DIRDAGL"
     gene            complement(2352..2606)
                     /gene="nusB"
                     /locus_tag="PKV52_01865"
     CDS             complement(2352..2606)
                     /gene="nusB"
                     /locus_tag="PKV52_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPD98992.1"
                     /translation="MQPLAPDWPIDKIARIDRVILRMGLYELKYEKDVPPKVVINEAV
                     ELAKAFGGENSSKFINGVLGTSYRAIEAQKPKKASSKKAK"
     gene            complement(2594..2815)
                     /locus_tag="PKV52_01870"
     CDS             complement(2594..2815)
                     /locus_tag="PKV52_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98993.1"
                     /translation="MASNRHLGRIVALQTLYEAEFRISCNDAEFEMSSVLDRNIARYS
                     ETIEDKDFIVSLVTGVQDKKPKLVVSCNH"
     gene            complement(2854..3003)
                     /locus_tag="PKV52_01875"
     CDS             complement(2854..3003)
                     /locus_tag="PKV52_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98994.1"
                     /translation="MGKPKKRTTPRRTGARRSHEVVKLARQVNAKSPVKAYTTRRESG
                     KKADK"
     gene            3136..3492
                     /locus_tag="PKV52_01880"
     CDS             3136..3492
                     /locus_tag="PKV52_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD98995.1"
                     /translation="MSNAYPKHIKNTPNQQPSDERTGNLKKIIGAVAIGLVLFVGGAK
                     LVDAARPDYGFSKKTHTEVAHPGDTPWSIASNIEGADQIDTRLVVEYLIETNPNLSDG
                     LQLGEAVEVPNKVKVN"
BASE COUNT          936 a          817 c          721 g         1319 t
ORIGIN      
        1 aaacagtttg gttactattg ttagtgccat aaaataaaac cacccggtgt ttatgccggg
       61 tggttttatc acataaagct cccttttgct taactttttt gaagcaatcg gctcgcactg
      121 agcttatttt agtgttttgg gatttatagg ttttagacaa gtcgtaaacg acttgattaa
      181 attatacgtc cagatcgtct aaatctgcaa gttcttggcg tgttttgctt acaacggaac
      241 tgtgggaact gctatcttct gagtcagtcg tttcgtcctt atcatcagta ttgtctgatg
      301 tagctggtct tactccacca cttggatcta caatttttaa gttataacga gcatcatttt
      361 tcgtaccaag tgcgcgcagt aagctacgaa tactctgtgc ggtagaccca cgcttaccaa
      421 tcactcttcc taaatcttct ggtgcaactg ttaactctag taatactccc ttttcatcaa
      481 tagtccttgt gacttctact gcatctggat taccgaccag tgattttact atatattcta
      541 caaattgttg atcaattgac gacatattct gacatcctcc gtattaatac tgtacttact
      601 agtatagcaa aaagctcacc tttatacatc aattgtgagc tcttaaaatg gtcaactact
      661 tatgcgtctg caggtgctgg agcttcttct gggaggtctt tggctacttc ttcggcaact
      721 gtctcttgct cgggtgcggg ctggttggcg cgtaatttat ctgcttttcg tactttgcga
      781 ttttttactg tacgctcttt tacccaacta ggtatgacaa caccttcttt tttgaatatg
      841 ctcacaactc gttcagacgg ttgcgcgcca ttattcagat atgtctgtga taaatctttg
      901 tctagtgtta attctttggt gtgtgggttg tatgagccta attttgccac tactttacca
      961 ctggtagggg tacggcgtga gtcttgtaca actacacggt agtggggcat ttttttacgc
     1021 ccagtacgct gcattctaat tgctaacata tgcctcttta aaacgtttaa cgattaataa
     1081 taagttccga acgtatagtc tatctgtttt tagccttaaa gtcaagcaga tgacccctat
     1141 gattttggtt ctggggtttt attggtatag cttgccagtg ctaactctgc agcctgttgt
     1201 tgagcagctt gtttgctggg gccggcaccc tgccctttta attgactatc cacaaataca
     1261 ccaaccgtaa acaacttttc gtgatctggg ccttcttctg ataagacttt atactgaggc
     1321 gttgcattat ctacactttg agcaatctct tgtaaacgtg actttgggtc catccatgaa
     1381 ccagttgcca gtattgtttc aaaggtggtc agcaagctgt tacttataaa tgtgctggcg
     1441 gcatcatagc cttgatcaag atagatagcg cctattaccg cttcgtagca gttcgctaat
     1501 atttgctgcc gagcacggtc actaccgcgc ttttcacccc tagacagtcg taaataatca
     1561 ttaaagccaa gtcgctcacc agctgcacta atactttctg tccgcaccaa cgcactacgc
     1621 cagttggtta gtataccttc tggctcagtg taattgcgat ataaatattc ggttactacc
     1681 agttctagta ctgcatcacc caaaaattct aagcgttcgt tatgttcttt taccgtcttt
     1741 ttgtgctcgt ttaaatacga acggtgagta aatgccgtca ccaacaacat gacgtcagaa
     1801 aatttgccgc cgagtacctg gctagcgagt gtttgatagt tactataatc ctgcatctct
     1861 aatatccttt aagcccagta gtattaactt accaatatct tcatattcta ttttttctag
     1921 cgcatcgttc gcatcgagcc atgcaatact tttcataaag tcttctttac ggatgttgtt
     1981 tgtgtcaccc tttgcctgca ctaaatatat gtgagtagtc ataagaatca ggctattttg
     2041 gcggcgatat tgaaaattta tcttgcctaa ccagttcatc actttcatct cttgcagccc
     2101 tgtttcttca cgtatttctc gttctgcagt ttcacgggct gtttcacctg gttctatatg
     2161 cccttttgga atcgtccacc gttcttttgg gtctttgtgg agcaatattt ctatcttatt
     2221 tttcttattt ctacgccata caataccacc agccgtagcc tctttgacta cttctgtaat
     2281 acttgggcgt ttccgatgaa agtatttttt aaaaccttct ataggttttg gtctattgat
     2341 tgccatgcat attactttgc tttcttggag gaagcttttt tgggtttttg ggcctctatg
     2401 gctctataag aagtaccaag tacaccattt atgaacttag atgaattttc accaccaaat
     2461 gcctttgcaa gctctacggc ttcgttaatg acaacttttg gtggaacatc tttttcgtat
     2521 ttaagttcat aaagtcccat tcgtaagata acacgatcaa tccgcgctat tttatcaatg
     2581 ggccaatctg gcgctaatgg ttgcagctca ccaccaattt cggcttcttg tcttgcacac
     2641 ctgttactaa actcacgata aagtctttat cttctatcgt ttcgctatag cgtgcaatgt
     2701 ttctgtctag cacagaagac atctcaaatt ctgcatcatt gcagctaata cgaaattctg
     2761 cttcgtatag tgtctgcagt gctactattc ttcctaggtg tctatttgat gccatgctaa
     2821 ctgctttctg gcgtgcttat aaagtgcgag ccgttactta tctgcttttt tgccgctttc
     2881 gcgacgagtt gtgtacgctt taacaggtga ttttgcattc acttgtcttg ctagtttaac
     2941 aacttcgtgg ctacggcgtg ccccagttct gcgaggggtg gtgcgctttt ttggcttacc
     3001 caaggtgcta actcctaaag ttatctataa atttaattga tattcttggc tattatacca
     3061 gcaccttatc tgctttacaa gataagcttt atcatatact tgcttattaa taacgtttat
     3121 gctattatac ttatcatgag taacgcatac ccaaaacata taaaaaatac accaaatcaa
     3181 caaccctcag acgaacgtac tgggaattta aaaaaaatca ttggagccgt agcaataggt
     3241 ttagtgctat tcgttggcgg tgctaaattg gtagatgctg ctaggcctga ctatggtttt
     3301 agcaaaaaga cccataccga ggtagctcac cctggtgata caccctggag tattgcttca
     3361 aacattgaag gtgcagatca aatagacact cgtttagtag tagaatattt aattgagact
     3421 aacccaaatc ttagcgatgg tttgcaatta ggcgaagctg tagaagtgcc aaataaagtt
     3481 aaagttaact agtaatttta gggttttaca tcttttgtat atttggtagc tattattcac
     3541 cattcagctg gtatttgata attacagcaa tagttcaata gtatgctctt gctcgcaaca
     3601 cgagagattt attatattac agacgcacct taaacgacga agttgatgag ttttttgggt
     3661 acgtatatga cctttttggg gtcacttttt atataggtgg caacgttttg atgaatacgg
     3721 gcttcttgct cgatggctac tttactagca ctgtgttcaa cttctatttc gccccttaac
     3781 ttgccgttga ctt
//
LOCUS       DAOPLS010000054         3782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_26824_length_3782_cov_3.757177, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000054 DAOPLS010000000
VERSION     DAOPLS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26824_length_3782_cov_3.757177"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..111
                     /locus_tag="PKV52_01885"
     CDS             7..111
                     /locus_tag="PKV52_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD98996.1"
                     /translation="MNYSLPQTYDDYVHRIGRTGRAGKTGYALTFISN"
     gene            131..508
                     /locus_tag="PKV52_01890"
     CDS             131..508
                     /locus_tag="PKV52_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HPD98997.1"
                     /translation="MSEPLNNANQTIAKKEELVAELRAQLNNFRLLAVVVIMIVTIST
                     WFFHVVEKWDWLDSVYYVVVTIATVGYGDYTPKTDIGKIYAICLIIVGIATFGFFAQQ
                     LLKRQQLRALERQLGRTEKKKQT"
     gene            541..617
                     /locus_tag="PKV52_01895"
     tRNA            541..617
                     /locus_tag="PKV52_01895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Arg,seq:tcg)
     gene            complement(715..1254)
                     /locus_tag="PKV52_01900"
     CDS             complement(715..1254)
                     /locus_tag="PKV52_01900"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPD98998.1"
                     /translation="MKDAPFKSFGIKLKQIRSHAKETLVDVSGAVETDVTLLEKLEAG
                     THQPSEDLVLLLISHFALKEDEALRLWELAGYNLEKTGTSSINTDDSEQSVQKAYVTA
                     GDVRILYTDMVHVKSNRHGVVINFLQSLGSDDHTMAVARVGMSHAHAQSLLEVLQQTL
                     NATNKQQKLLDTNDSSNKS"
     gene            1340..2356
                     /gene="murB"
                     /locus_tag="PKV52_01905"
     CDS             1340..2356
                     /gene="murB"
                     /locus_tag="PKV52_01905"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPD98999.1"
                     /translation="MEILKNQPLASHITMKLGGDASYMAEVTTESDLQEAIAFAKERS
                     LNVMVLGGGSNIIFGDKGYDGLVIINEIKGVTIDEQSGVVRAGSGENWDDLVAASVAH
                     NLAGIEALSLIPGTVGAAPVNNIGAYGQEVKDTITRVRAYDTQANAMVELTNKECQFS
                     YRNSVFKTLQYGRYIITSVTFQLHVADDAYSPPSYASVQTMLQNQNITEPSVKTIRDI
                     VINIRKAKLPDPAVLANTGSFFKNPIVTKDICNNLLLSYPTMPYFEHNNNVKLAAGWL
                     IEAAGLKGLSKDGIRVYDKQALVLVNDGTRSFQSLLNMKNLIQQTVFDKFGVMLEPEP
                     EIIA"
     gene            2353..>3782
                     /locus_tag="PKV52_01910"
     CDS             2353..>3782
                     /locus_tag="PKV52_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPD99000.1"
                     /translation="MKISRRAFFKYIKKELPTVTFEEIVTIERAYEFAKTAHAGQTRA
                     NGDPYFQGHCVPVAMHVIDLGMDSTLICAALLHDTIEDTSVTVEDIQTEFGDDVASLV
                     EGVSKLGKLKYRGNERHVESLRKFFVSIARDVRVVILKLADRWHNLETLQYLPEEKQK
                     RIALESIMIHAPLASRLGMGKLVTTINDLAFPYAYPEEYTKTKKLMVARLKKAEGTIK
                     KIYRGMLVTVNASIGYTPQIDKRVKGTYSLYKKLERKHWNEDEIFDIVALRVITHTVA
                     DCYSALGAVHNHWRPVPGRIKDYIAVPKPNGYQSLHTAIFSGDGPIVEVQFRTQEMHE
                     FNEYGVASHHSYKNTTLQKGIYKETFAWIEQLREFQNAELTPSDYLKRLKTDFFQDRI
                     FVFTPKGDVIDLPFGATLLDFAYAVHTQIGEHANGGKINGKYMALKTILHNEDIVEVD
                     TNSKSHPSDKWLDMCITSNAQGRIKR"
BASE COUNT         1159 a          730 c          817 g         1076 t
ORIGIN      
        1 catgttatta actactcttt acctcagact tatgatgatt acgtacaccg tataggacgt
       61 acaggccgag ccggcaaaac aggctatgcg cttaccttta taagcaatta gtgttgtata
      121 ctgtactctt atgagtgaac cattaaataa cgcgaaccaa acaattgcaa aaaaagaaga
      181 actagttgcc gagttacgtg cacaattaaa taactttaga ctgctggcag tggttgtaat
      241 tatgattgta acgattagta cttggttctt ccatgttgta gaaaaatggg attggcttga
      301 ttctgtgtac tatgtagtag tcactatagc taccgtaggg tacggcgatt acacaccaaa
      361 aactgacata ggtaaaatct atgctatatg tcttattatt gtaggcatag ccacgtttgg
      421 attttttgcc cagcaacttt taaaacggca acaacttcgg gcgcttgaac ggcagttggg
      481 gcgaacagaa aaaaagaagc agacctaaac gcctgccatc tgttataata cctaacgcat
      541 gcacccgtag ctcagcggat tagagtactt ggcttcgaac caaggggtcg caggttcgaa
      601 tcctgccggg tgtaccacgt ctatatttaa gggcttctct atagaagtct tttttgtaac
      661 ttgtgcagac gttttcttaa aaaatagtat agccgatttg ttgtagttgc tctgttatga
      721 tttgttacta gaatcgtttg tatctagcag tttttgttgc ttattggtag cgttaagagt
      781 ctgctgtagc acttctaaga gtgattgggc gtgtgcgtgg ctcataccta cccgcgcaac
      841 agccatagtg tgatcgtcgc taccaagtga ctgtagaaag ttaatgacaa cgccatgccg
      901 atttgattta acatgtacca tatcggtata caatattctg acgtcccctg ctgtaacata
      961 ggccttttgc acactttgtt ctgaatcatc ggtattaatg ctagacgtac ctgttttttc
     1021 tagattatag ccggctaatt cccacagacg cagagcttcg tcttctttta gcgcaaaatg
     1081 gctaatgagg agcaaaacaa gatcttcgct cggctggtgt gtacctgcct ctagcttttc
     1141 tagcaacgtt acatctgttt ctacagcacc agatacatct accagcgttt ctttggcatg
     1201 tgagcgtatc tgtttaagtt tgattccgaa cgatttgaac ggtgcatctt tcataaaaat
     1261 gtctccgtga ttattactat attatactat gttttttatg ttctgcgcag atttacgtta
     1321 tagattatac tagtaccata tggaaatact aaaaaaccaa ccacttgcat cacacattac
     1381 tatgaaactg ggtggtgatg cttcgtacat ggctgaagta acgacagagt ctgatttgca
     1441 agaggccatc gcatttgcca aagagcggtc actaaacgta atggtacttg gcggtggttc
     1501 taatattatt tttggagaca aaggttatga tgggctggtg ataatcaatg aaataaaggg
     1561 tgtcaccata gacgaacagt cgggtgttgt acgcgcaggg tctggtgaaa attgggatga
     1621 tttagtagca gcgtctgttg cgcataatct tgccggcata gaggctttat cgttaattcc
     1681 aggtacagtt ggagctgctc cggtaaataa tattggcgca tacggccaag aagtaaaaga
     1741 caccattacg cgtgtgcgcg cctacgacac ccaagcgaat gccatggttg aactaacgaa
     1801 caaggaatgc caatttagtt atagaaatag cgtgtttaag acactgcagt atggcaggta
     1861 cattattacc agtgttacat ttcagttaca tgtcgccgac gatgcatata gcccaccaag
     1921 ctatgcttct gtgcagacaa tgctccaaaa ccaaaatata accgagcctt ctgttaaaac
     1981 aatacgtgat atcgtcatta atattcgtaa agccaaacta cctgatcctg cggtgctggc
     2041 gaacactgga tcatttttta aaaaccctat cgttactaaa gatatttgca ataatttact
     2101 tttatcgtac cctactatgc cgtactttga gcacaacaat aatgttaagt tggcggctgg
     2161 ctggcttata gaagcagctg gcttaaaagg gcttagtaaa gatggtattc gcgtgtatga
     2221 taaacaagcg ctcgtgctgg tcaatgatgg cactcgctcc tttcagtcac ttttaaatat
     2281 gaagaatctt attcagcaaa cggtgtttga taaatttggg gttatgttag agccagaacc
     2341 agaaattatt gcatgaagat aagtagacga gcctttttta aatacattaa aaaagaacta
     2401 ccaactgtaa catttgaaga aatagttacg atagagcgag catatgagtt tgcaaaaaca
     2461 gctcacgctg ggcaaacgcg agcaaatggc gacccctact ttcaggggca ctgtgtgcca
     2521 gtagctatgc acgttataga tttaggtatg gatagtactc ttatttgcgc agcgctcttg
     2581 cacgacacca tagaagatac gagcgtgacg gtagaagaca ttcaaacaga gttcggcgat
     2641 gatgtagcta gcttggtaga aggtgttagt aaattgggta aattaaaata tcggggcaat
     2701 gaacgtcatg tagaatcttt acgaaagttt tttgtttcta ttgctcgtga tgtgcgagtg
     2761 gtgatattaa aactggcgga ccgttggcat aatttagaaa cattacaata tctaccagaa
     2821 gaaaagcaaa aacgcattgc actagaaagt atcatgatac acgcaccact cgcatcacgt
     2881 ttaggtatgg gtaaattagt aacgactatt aatgatcttg cctttccgta tgcctaccca
     2941 gaagaatata caaaaacaaa aaaattaatg gtagctaggc taaaaaaagc agaaggcacc
     3001 attaaaaaga tatatcgtgg catgttagta accgtcaatg catctatagg ctatacacca
     3061 caaatagata agcgtgttaa ggggacatat agcctatata aaaaattaga aagaaagcac
     3121 tggaacgaag acgaaatatt tgatattgtc gcgctgcgtg tcattacaca taccgttgcc
     3181 gattgctata gtgcgcttgg tgcagtacat aatcactggc gacctgtacc aggcagaatt
     3241 aaagactaca ttgctgtacc taaaccgaac ggatatcaaa gcctacacac cgcaattttt
     3301 agtggagatg ggcctattgt cgaggtgcag tttagaacac aagaaatgca cgaatttaat
     3361 gaatacggtg ttgccagtca tcatagttat aaaaacacta ctcttcaaaa aggtatctac
     3421 aaagaaacgt ttgcctggat agaacaactt cgggagtttc agaacgccga actcacgccg
     3481 agcgattatt taaagcggct taaaacagac ttttttcaag acagaatttt tgtatttact
     3541 cctaagggtg atgtgataga tctgccattt ggagctacgt tacttgattt tgcctatgca
     3601 gtacataccc agattggtga gcacgctaac ggtggcaaaa ttaatggtaa gtacatggca
     3661 ctaaagacta ttttgcataa tgaagatatc gtagaagttg atacaaacag taaatcccac
     3721 cccagcgata aatggcttga tatgtgtata acatctaacg cacaaggccg catcaaacga
     3781 ta
//
LOCUS       DAOPLS010000055         3765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_26996_length_3765_cov_3.715903, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000055 DAOPLS010000000
VERSION     DAOPLS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26996_length_3765_cov_3.715903"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="metK"
                     /locus_tag="PKV52_01915"
     CDS             complement(<1..1028)
                     /gene="metK"
                     /locus_tag="PKV52_01915"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPD99001.1"
                     /translation="MSKHVKVSESVCAGHPDKLADQISDAILDAALTQDAYARVAAET
                     LVAKDTIILAGELSTTANLDFDSIARNVIKDNGYTHPDWGFSDRSVIINKLHQQSKEI
                     SIGVDDDGAGDQGLMYGYATNETDQYLPLPLVLSHTICRLLDEYRVNKTIPYLRPDGK
                     AQVVVEYDGYKPIAVKHITIAVPHDEAVTLDTVKDDLLKLIITPILHEYTLHTPNDII
                     VNGTGVWHNPGPASDVGLTGRKIVVDTYGGAAKVGGGAFSGKDPSKVDRSGAYAARYI
                     AKNIVANGLADRAEVCLAYYIGAKSPIIQTIDTFGTAHVSEESLRNAMHNLLDCSVKN
                     VIEKLDLR"
     gene            complement(1264..2916)
                     /locus_tag="PKV52_01920"
     CDS             complement(1264..2916)
                     /locus_tag="PKV52_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPD99002.1"
                     /translation="MNDLKQQISNIIEGEVDDSPEVKEFYSHDASMFELAPQIVAYPK
                     NAADIKKIVSFVAENKKNQPELSVTVRSGGTCMSGGAVNDSIVLDVQKHMNAIGDVSP
                     TKASAQPGMWYRDFEKETLKVKAIMPSYPASRDLCTVGGMVANNAGGEKSLQYGKTEK
                     YIDQLKVILGDGNEYIVKALNKSELDAKIAQNDYEGNIYKQLFELVDNNYDEIKNAKP
                     HISKDSTGYHIWDVWDRETGIFDITQVMVGSQGTLGIVTDVTFRLVPAPTHSGTLVVF
                     LRKIDDLGDLINKVLEHKPATFEGFDNYTLMLSFKLFFYFHKTLGWAGLTKLAFQLIP
                     DALMLFRGIPKMVLLVEFNGDTQDEVRQKVHHMREDLKPFGHEALFEEDGGQDQAKKF
                     WIMRRESFNLLRKKVKNKHTAPFMDDLIVPPQTLPHFLPEVRAIINKYKLLATIAGHM
                     GDGNFHIIPLMKIEDEKEKAKLEPAMHEINELVLKYGGSLSGEHNDGMIRGPWLKDMY
                     GPAVFAYFQTVKNIFDPENIFNPHKKTDSDWQFSMAHLREHF"
     gene            2985..>3765
                     /locus_tag="PKV52_01925"
     CDS             2985..>3765
                     /locus_tag="PKV52_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD99003.1"
                     /translation="MLKKHVVIVGGGWAGIRLARKLKKLAKNNHIAVTLVSNESNFRY
                     SAALYRVATGHKEKEALLPIHELLADLPDTKFTKATMTNIDPHKRTITLSKGKIVHYD
                     YAVLALGSVTTYFGIPGIAEHAHSIKTQKELRRFRTHIHQELLNEHAPDKHYVVVGAG
                     PTGVELAAAMSSYMRSVVKRHGLRKRRIHIELVEASSRVLPMSHPKASKITLQRLRLL
                     GITVHLNSKVEYESDTGLVVNGRTIPTKTVIWTAGVANNPFF"
BASE COUNT         1051 a          846 c          697 g         1171 t
ORIGIN      
        1 cttcttaagt ccagtttttc aatcacattc ttaacagagc aatctaaaag attatgcatc
       61 gcgttacgta aagattcttc agaaacgtga gcagtaccaa acgtatcaat tgtctgaata
      121 attggggact tcgcgccaat ataatatgct aaacacactt cagccctgtc ggctaagcca
      181 ttcgcaacta tgtttttggc aatatatctc gctgcatacg cacccgacct atcgacttta
      241 ctaggatctt taccactaaa cgctcctccg cctacttttg cagcaccacc atacgtatct
      301 acgactattt tgcgccctgt taaacctacg tcactggcag gccctgggtt gtgccataca
      361 ccagtgccat tcacaataat gtcgtttgga gtatgtaatg tatattcgtg caatattgga
      421 gtaattatta attttaatag gtcatcttta actgtatcaa gcgtaacagc ctcatcatga
      481 ggcactgcta tggtaatgtg cttaacagca atcggtttat agccatcata ttctacgact
      541 acttgtgctt taccatcagg ccttagataa ggtattgttt tgttcaccct atactcgtct
      601 aataatctgc atattgtgtg cgataacacc aaaggtaatg gcaagtactg atctgtttcg
      661 tttgttgcat acccatacat caatccctga tctccagccc cgtcatcatc tactccaata
      721 gaaatctctt tagactgttg atgtaattta tttataatta ccgaacgatc gctaaagccc
      781 caatcggggt gcgtatagcc attgtcttta attacattgc gggcaataga atcaaaatct
      841 aagtttgcgg tcgtacttag ttcaccagca agtataatgg tgtctttggc cacaagagtc
      901 tcggcagcaa ccctagcata tgcatcctga gttaatgcgg cgtctaatat agcatcacta
      961 atctgatctg ctaatttatc tggatgccca gcacacacag actcactcac ctttacatgt
     1021 tttgacataa aaaaatcctt tcttttagcg aagcagcacc acgtattgta tgcatgctcc
     1081 taagaaagga ttctatacat atctttccgt accaaagcgg actagatggg gcaccgttcg
     1141 cgacgtgcga tggttgccgg tgggttactg agctagttct ctacccacac tcgtgatatt
     1201 ttgtgcttct aactattaat gaacgcttat gattgtacca aatacattgt attcaaggca
     1261 actttaaaag tgctcgcgca agtgggccat actaaactgc caatcagaat cagtcttttt
     1321 gtgtggatta aagatatttt ctgggtcaaa tatattttta actgtttgaa aataagcaaa
     1381 tactgcaggg ccatacatat cttttagcca gggcccgcgt atcataccat cgttatgctc
     1441 accgcttagg ctgccaccgt attttagtac taattcatta atttcatgca tggctggctc
     1501 taatttagct ttttcttttt catcctctat cttcataagc ggaatgatat gaaagttacc
     1561 atcgcccatg tgccctgcaa ttgttgccag taatttgtac ttattaataa ttgcccttac
     1621 ttcaggcaaa aagtgaggta aggtttgtgg tggcacaata aggtcatcca taaatggggc
     1681 ggtgtgcttg ttttttactt ttttgcgcaa taagttaaaa ctctcgcggc gcataatcca
     1741 aaatttttta gcttggtctt gcccaccgtc ttcttcaaat agcgcttcat gcccaaacgg
     1801 ctttaagtct tcgcgcatgt ggtgtacttt ttgtctgact tcatcttgag tgtcaccatt
     1861 gaactcgacg agcaatacca tctttggaat cccgcggaat aacatcagtg cgtctggtat
     1921 tagctgaaaa gctagtttcg tgagccctgc ccagcctaga gttttgtgaa aatagaagaa
     1981 taatttaaaa ctgagcataa gtgtatagtt atcaaaccct tcaaatgtgg ctggtttatg
     2041 ttcaagaact ttattaatta aatctcctag atcatcgatt tttcgtaaga atacgactaa
     2101 agttcctgag tgtgtcggtg caggtacaag ccgaaacgta acatctgtaa caatacccaa
     2161 cgtaccttgc gatcccacca tgacttgggt aatatcaaaa atacccgttt ccctatccca
     2221 cacatcccaa atatggtacc ccgtagaatc cttacttatg tgtggtttgg cgtttttgat
     2281 ttcatcataa ttgttatcta cgagttcaaa taattgttta tagatatttc cttcgtaatc
     2341 attctgagct atttttgcat caagttcact tttatttagt gcttttacga tgtattcatt
     2401 gccatcacct aaaataactt ttagttggtc tatatatttt tctgtcttac cgtattgaag
     2461 agacttttca ccaccggcat tattcgcaac cataccccca acagtgcata aatctcttga
     2521 cgcaggatag ctgggcataa ttgcctttac ttttagtgtt tctttttcaa aatcgcggta
     2581 ccacatacca ggctgggcac tagctttcgt gggtgaaacg tcaccaatgg cgttcatatg
     2641 tttttggaca tcaagcacga tagagtcatt gactgcacca ccagacatgc atgtcccacc
     2701 actgcgtaca gtcacagata attctggctg gtttttctta ttttcggcca caaacgatac
     2761 aatttttttt atatccgcgg cgtttttagg ataggctacg atttgtggag caagttcaaa
     2821 catacttgcg tcatggctat aaaattcttt cacttctggc gagtcatcaa cttcaccttc
     2881 aataatgttt gagatttgtt gttttaaatc attcatcgct ttttactgta tcacttgctt
     2941 aaacataagc gcaactacat gatttgttgt acaatacttt atatatgcta aaaaaacatg
     3001 tcgtcattgt tggcggcggg tgggcaggca tccggcttgc aagaaaactt aaaaaactcg
     3061 ccaagaataa ccacatagct gtcacgctcg tatctaatga atctaacttt agatatagcg
     3121 ctgctctata tagggtagca acagggcaca aagaaaaaga agcactgctg cctatacatg
     3181 agcttctcgc ggatttacca gatacaaaat ttacaaaagc tacaatgaca aatatagatc
     3241 ctcacaagcg cactattacg cttagtaaag gcaaaatcgt tcattatgat tacgcagtct
     3301 tggctctagg ttcggtcaca acatattttg gtattcctgg tattgctgaa catgcccaca
     3361 gtataaaaac ccaaaaagag ctacggcgct ttagaacaca tattcaccaa gaactcctaa
     3421 acgaacatgc accagataaa cactacgtcg tcgtaggcgc aggccctact ggtgtcgagc
     3481 ttgcagctgc gatgtcgtcg tatatgcgtt cagtcgtaaa aaggcatggt ctccgaaaac
     3541 gtcgtatcca cattgaactc gtagaggctt cttctagagt actgcctatg tcacacccta
     3601 aagcttcaaa aattaccttg caacgcctac ggttacttgg cattactgta cacctaaata
     3661 gcaaggttga atacgaatcg gacacgggct tagttgtgaa cggtcgtact atcccgacaa
     3721 aaacagtcat ttggacagct ggcgtggcca ataacccatt ctttg
//
LOCUS       DAOPLS010000056         3760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_27046_length_3760_cov_3.369501, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000056 DAOPLS010000000
VERSION     DAOPLS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27046_length_3760_cov_3.369501"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PKV52_01930"
     CDS             complement(<1..1187)
                     /locus_tag="PKV52_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPD99004.1"
                     /translation="MGTQKLKRELGLPILTLYGLGNILGAGIYVLVGKVAGQAGTSTT
                     LAFLIAMSIAAITAFSYMELSGRYPVSASASVYTFKAFASKRLSMIIGVAMVLSGVAS
                     AAALSKGFAGYVNELIAIPAVVVSILLLVAIGLLAVRGIGESAKAAALFTIIEVVGLL
                     IIIYIGKGAIANYDYHTILTIDPSVGWAGVMSGAFLAFYAFIGFEDMVNVSEEAKSPR
                     RTMPLAVLLSLLGATVLYILVVIVATTLVSPSELAATNAPLTLVFQAGGYSASEIITI
                     IGMAATVNGVVVQIVMGSRMLYGMAKQKWINRRFASVHSTYQTPAFATFVVVGLMIIG
                     VIALPLVSLASVTSMLVLAIFATVNAALIVIKKQHPKNPGYISVPILLPWLGVISCAG
                     ILV"
     gene            1363..2361
                     /locus_tag="PKV52_01935"
     CDS             1363..2361
                     /locus_tag="PKV52_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPD99005.1"
                     /translation="MKKQYSPYGKYMWDTWFAIDSDGTYHAFYLESDVGDTRYATPSI
                     GHAKSKDLKNWEQLDTVLEPGNEGDWDDLALWTGSVFKHKDKWYMFYTGRNKRDQWQQ
                     AIGLAISDDLYHWQKVPGPVLEPGDSRYSPQTDFNEIDNPPAWRDPFIFYDDVTEKFH
                     MFFTARVNNGNTSFNGCIGRATSDDLLRWQTEEPVIVNEGFDEMEVPQMLYIKNRYYL
                     LFSYRKSHELKNKPKLSSGLYCFVSNEIGGGYRPIKGWYGQIMKYGNKYYGMRLIKQK
                     SNKHHFAIGWHHLKRGEFVGKMTDVYTVRLEPHAFSITDFSINIVDTFKKITQPKK"
     gene            2545..2793
                     /locus_tag="PKV52_01940"
     CDS             2545..2793
                     /locus_tag="PKV52_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99006.1"
                     /translation="MSEKKGDCLLQNGPWSHRELHNSDDETPDALGKFCVVQGCHRAL
                     IAANGKSHQSSTCLRGLVYSSDISNNQQHTISVNPEYL"
     gene            complement(2822..3331)
                     /locus_tag="PKV52_01945"
     CDS             complement(2822..3331)
                     /locus_tag="PKV52_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPD99007.1"
                     /translation="MAIDEKTGLPNDSKIISVPGYLKLGKIITWLLYIWIIFGVVMLA
                     LRTFLLATSANPTTFVDFVYRTSADFLQPFRGIFPSKPVGETGYLDVSAIFAIIIYLI
                     FAWLVSAGIKYIQAKIDDIKEQEAERIERLQAKQMLEQRSQIVKQTTYVNKTPASRQT
                     AQSQPKKRM"
     gene            3423..3725
                     /locus_tag="PKV52_01950"
     CDS             3423..3725
                     /locus_tag="PKV52_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HPD99008.1"
                     /translation="MKAVWNNQVLAEAPKEDLIFIEGNLYFPPSALHKEFFTANDEHT
                     SCFWKGQASYYDIIVGDKTNKSAAWYYPKPMDGSIEKVKKDFTNYVAFWRGVSVEE"
BASE COUNT         1209 a          763 c          774 g         1014 t
ORIGIN      
        1 tacacaagta tacccgcgca gcttatcact cccaaccaag gcagcagtat tggcacacta
       61 atgtatccag ggtttttagg atgttgtttt ttaatcacaa tcaaggcagc attaactgtc
      121 gcaaaaatag ctaaaacgag catacttgtt actgatgcga gcgagacaag aggcaaggct
      181 attacgccga ttatcatgag cccgacaacc acaaaggtag caaaagctgg tgtttgatat
      241 gtgctatgaa cactcgcaaa acgccgattt atccattttt gctttgccat accatatagc
      301 atgcgactgc ccattactat ctgtactacc actccattca ccgttgcagc cataccaata
      361 attgttatga tctctgacgc agaatagcca cctgcttgaa acactaaagt gagaggggca
      421 ttggtagcag ccaattcact aggacttacc aaggtggtgg cgacaatcac gacaagtata
      481 tatagtaccg tggcacccaa cagagataat aagactgcaa gaggcatcgt acggcgtggt
      541 gacttggctt cttcagacac attcaccata tcttcaaagc caataaaagc ataaaatgcc
      601 aaaaatgcgc cgctcattac tccggcccaa ccgacggaag gatcaatagt caatattgta
      661 tggtaatcat aattagcaat agcacctttg ccaatgtata taatgattaa aagaccaacg
      721 acttcaataa tagtaaatag tgcggctgct ttagcagatt cgccgatacc gcgcacagcc
      781 agtaaaccaa tcgccactag gagtagtatg ctaacgacaa ccgctggtat agcgataagt
      841 tcatttacgt acccagcaaa gcctttagaa agtgccgccg ccgaggcaac accacttagt
      901 accattgcta ccccaataat catagagagg cgtttgctgg caaatgcctt aaacgtgtat
      961 acagaagcac tcgcacttac tggatatctg cccgaaagct ccatgtaaga aaatgctgtt
     1021 attgctgcga tactcatagc aatcaaaaag gcgagggtag tgcttgtacc agcttggcca
     1081 gcaactttgc caacgagcac atatattccc gcacctaata tatttccgag cccatacagc
     1141 gtaagaatag gtaaaccgag ttcgcgtttt agtttttgcg ttcccacata tgtatggtac
     1201 cacttttttg gctacgttta attggctcgt atccatgtga gtgcatttaa aaccgtataa
     1261 atacacgacc catgctggca aacgtaggta cacaataggt ttatagcatg tgtaatttgg
     1321 tatggatatt ctgtttagta tgaactataa tcaatcaacg ttatgaagaa acaatacagt
     1381 ccatacggta aatacatgtg ggacacgtgg ttcgccatag attcagatgg cacgtaccat
     1441 gcgttttatt tagagtcaga cgttggtgat actcgatatg caaccccctc tataggtcat
     1501 gctaaatcta aagacttaaa aaattgggaa caactagata cggttctgga gccaggcaat
     1561 gaaggtgact gggacgattt agcactatgg acggggtctg tgtttaaaca taaagacaaa
     1621 tggtacatgt tttacacggg cagaaacaaa cgtgaccagt ggcaacaagc aataggtctt
     1681 gccattagtg atgatctata ccattggcaa aaagtcccag ggcctgtgct agaacccggt
     1741 gattcccgat attcacccca gaccgatttt aacgaaatag ataatccacc tgcatggcgc
     1801 gacccattta ttttttacga tgatgtaact gaaaaatttc atatgttctt tactgctcgt
     1861 gtaaacaacg gcaatacgag ttttaatggc tgcataggga gagcaaccag cgatgattta
     1921 ttacgttggc aaacagaaga accggtaatc gtaaacgaag gctttgatga aatggaagtg
     1981 ccacaaatgt tatatattaa aaaccgctat tatttgttat tttcatacag aaaatcgcac
     2041 gaattaaaga ataagcccaa gctaagtagc ggtctatatt gctttgtttc aaatgaaata
     2101 ggcggtggat atcgtcctat caaaggttgg tatggccaaa ttatgaaata tggcaataaa
     2161 tactatggca tgagattaat caaacaaaaa agtaataaac accactttgc tatagggtgg
     2221 catcatttaa aaagaggaga atttgtgggc aaaatgaccg acgtctatac cgtaaggcta
     2281 gaaccacatg cgtttagtat tacagatttt agtatcaata tcgtagatac attcaaaaaa
     2341 ataacacaac caaaaaaata gcagcacggc acgttattac ataatagtca aagaatgggt
     2401 aaaaactgaa gggcacctgc ggcgatgcta gaaggcacta ctaagtgtct acgtgctgca
     2461 ttaacacaat tatttactag atgcacgcta cgggttcatt ctaaaagtat agtactgcgt
     2521 atattaatca ggtatcattg gtgaatgagt gagaaaaaag gggattgttt actacaaaat
     2581 ggtccatgga gtcatcgtga attacacaat agcgacgacg agacgcctga tgcccttggt
     2641 aaattctgtg tcgtacaagg ctgtcataga gcattgatag cggcgaacgg caaaagccac
     2701 caaagtagca cctgcctgcg tggtcttgtg tattcaagcg atatttctaa taatcagcaa
     2761 catacaatta gcgtaaaccc agaatattta taagtaactg ttttttctaa caactacata
     2821 gttacattct tttttttggt tggctctgcg cagtctgtct tgacgctggt gttttattaa
     2881 catatgtcgt ctgttttact atctgactac gttgttctag catttgcttt gcttgtaagc
     2941 gttcaatacg ttcggcttct tgctctttta tgtcatcaat ttttgcttgt atatacttta
     3001 ttccggcaga cactaaccat gcaaatatga gatagataat tattgcaaat attgcagata
     3061 cgtctaaata gcctgtttca cctactggct tagaaggaaa aataccacgg aatggctgta
     3121 aaaaatcagc cgacgttcta tatacgaaat ctacaaatgt tgtagggttt gcagaagtgg
     3181 cgagtaagaa agttcttagc gcaagcatca caacgccaaa gataatccat atatacagga
     3241 gccacgtgat aattttaccg agtttaagat accctggtac ggaaataatt tttgaatcgt
     3301 ttggtaagcc tgttttttcg tctatggcca tttggttact ccttttttaa tactataact
     3361 atagcacagt agacaggtga gtgaactagc aacaggcgaa agtatgtgag atactaatac
     3421 acatgaaagc agtctggaac aaccaagtgc tcgcagaagc tcctaaagag gatcttatat
     3481 ttattgaagg taacctatac tttccgccga gcgctctcca taaagaattc tttacagcaa
     3541 acgatgagca cacgagctgt ttctggaaag gccaggctag ctactatgac ataatcgtgg
     3601 gtgataaaac caacaaaagc gctgcttggt actacccaaa accaatggac ggctctattg
     3661 aaaaagtaaa aaaagacttt acaaattacg tagcattttg gcgcggcgtt agcgtagaag
     3721 aataataggt gagcgcatta cgcaagctat ttactgacaa
//
LOCUS       DAOPLS010000057         3725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_27399_length_3725_cov_5.473297, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000057 DAOPLS010000000
VERSION     DAOPLS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27399_length_3725_cov_5.473297"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKV52_01955"
     CDS             <1..387
                     /locus_tag="PKV52_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99009.1"
                     /translation="SATMSPRSFSPEVTLDADWQKSYKRQLEVYQWLLRQNGFTVSDT
                     GYFVYANARFDGDIFNDTLTFTTKLIAHTGTDNWIEPTLVHIKEVLEGDMPPVGDSIM
                     GGECEFCAYARKRTELTLDHLQKSKR"
     gene            416..1714
                     /gene="serS"
                     /locus_tag="PKV52_01960"
     CDS             416..1714
                     /gene="serS"
                     /locus_tag="PKV52_01960"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPD99010.1"
                     /translation="MVDIQFIRENPDLVKEKSKQKGYDIDIDELLRLDSERLALLAQV
                     EELRQKRNENAAAIKKQGKPDQAAIDTGKQIKVELAHREALLTETTEAWGIAHKQVPN
                     MPLETVPIGNSEDQNVVVKEVGTKPNFDFTPQNHAHIAESKGWLDKERATKIAGARFV
                     YMKGDLVLLEFALWQYGLSVLTNSTLLKQIATDNALSVSTKPFIPVIPPAVAKQSVFE
                     ATGRLNKQEQTYKIEDEDLWLNASAEHTLSPMYLHEILSENELPIRLVGYTTAFRREA
                     GTYGKDTEGIFRLHQFNKLEMESFSAPEDSLSEHHFMVAIQEYLMQSLGLPYRVLEKC
                     TADIGRPNAKGVDIDVWLPSQQAYRETHTADYLTDYQARAMNTRVRRADGHIELVHTN
                     DATAFSQRPLIGIIENFQTKQGDVRIPDVLKPFMSGKELI"
     gene            1711..2088
                     /gene="raiA"
                     /locus_tag="PKV52_01965"
     CDS             1711..2088
                     /gene="raiA"
                     /locus_tag="PKV52_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPD99011.1"
                     /translation="MIKKIEVTSIHTETEPEVTKYVKKKIAKLDSYMPKHARKSARAE
                     VRLKEEKIKTKKQSSCEVVLYVPGEIITAKETTVNMYAAVDIVEEKLKSQLRKYKEQT
                     IITKKRGDAKIRKMFGKIVGKSR"
     gene            2159..>3725
                     /locus_tag="PKV52_01970"
     CDS             2159..>3725
                     /locus_tag="PKV52_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPD99012.1"
                     /translation="MSNFIKKVLGDNQNGALRRMRRKVKEINKLEDTYKAMPKKTLAE
                     QTQVLQKRAEKESLDKILPDAFAVVREAARRILKQRHFDVQLIGGITLHDGNVAEMKT
                     GEGKTLVATAPVYLNALTKKGVHVVTVNDYLARRDAGWMGEIYHFLGLSTAVIIPDES
                     YIYDPEYTNEDHTDPRFQHLRPCTRQEAYQADITYGTNNEFGFDYLRDNMVKETNQLR
                     QRELNFAIVDEVDSILIDEARTPLIISAPSTTSGQSYTQFARVVAQLKPEHYVVDEKE
                     KQVVLTDKGIEKVEKLLGIKNLYIGDNIRTLYHLEQALRAQTLFLRDKDYVVTKDGEV
                     VIVDDFTGRLLKGRRYNEGLHQAIEAKESVTVQEESMTLATISFQNYFRLYNKLSGMT
                     GTAATEAEEFHQIYKLEVISIPTNKKNIRKDLPDRIYKTKEAKTRAIIKEVQRLHKKG
                     QPVLIGTVAIENNEEISAALNKAKIPHQVLNAKNNESEAQIVAKAGHVGAVTLATNIA
                     GRGTDIVLGDGVRE"
BASE COUNT         1277 a          775 c          804 g          869 t
ORIGIN      
        1 tcggcaacaa tgtcacccag aagtttcagc ccagaagtga cgctagacgc cgattggcaa
       61 aaaagctata aacgccagct agaggtatac caatggttat tacgccaaaa tggttttacc
      121 gtgagtgata cggggtattt tgtgtacgcg aatgctcgct ttgatgggga tatttttaac
      181 gatacactga cctttacaac caaattaatt gctcacacag gtaccgataa ttggattgag
      241 ccaacacttg tacacattaa agaggtactc gaaggcgaca tgccaccagt tggcgattct
      301 ataatgggcg gtgaatgcga attttgtgca tatgctcgca agcgtacaga acttacgcta
      361 gatcatttgc aaaaatctaa acggtaagct tgcctaaaag gggtataata aaagtatggt
      421 agatattcag tttattcgcg aaaatcctga cttagtaaaa gaaaaatcca agcaaaaggg
      481 ctacgatata gatattgatg aactactgcg ccttgatagc gaacgacttg ctttgcttgc
      541 acaagtagaa gaattgcgcc aaaaacgcaa cgaaaacgca gcagctataa agaagcaagg
      601 taaaccagac caagcagcaa tagacactgg caaacaaata aaagtagaac ttgcccaccg
      661 cgaagcttta ctcacagaaa ctaccgaagc ctggggtatc gcacacaagc aagttcctaa
      721 catgccttta gaaactgtac caattggcaa cagtgaagac caaaacgtgg tagtcaaaga
      781 agttggcacc aagccaaatt ttgattttac accccaaaat catgcccaca tcgccgaaag
      841 caaaggctgg ttagataaag aacgggctac caaaattgcc ggcgcgcgct ttgtgtatat
      901 gaagggtgat ttagtgcttt tagaatttgc attgtggcag tatggtctga gtgttctaac
      961 aaatagcaca ctccttaagc aaattgctac agataatgcc ttatctgtca gtacaaaacc
     1021 atttattcca gttataccac cagcagtggc taagcaatct gtttttgaag ctacagggag
     1081 gttaaacaag caggagcaaa cctacaaaat tgaagacgaa gatttatggc ttaatgccag
     1141 tgccgaacat acgctatcac caatgtattt acacgaaata ctatcagaaa acgagctacc
     1201 cattaggttg gttggctata cgacggcctt ccgtcgcgaa gcaggcactt acgggaaaga
     1261 tacagaaggt atttttagat tacaccagtt taataaatta gaaatggaaa gctttagcgc
     1321 acccgaagat tcacttagcg agcatcattt catggttgct attcaagaat atttgatgca
     1381 aagcctaggt ttgccgtacc gtgtcctaga aaaatgtact gctgatattg gccgtcccaa
     1441 tgctaagggg gttgatattg acgtatggct cccaagccaa caggcatacc gagaaactca
     1501 tactgcagac tacttaacag attaccaagc cagggcaatg aacacacgag tgcgtcgtgc
     1561 agacggccat atcgagcttg ttcacaccaa tgacgcgaca gcattttcac agcggccact
     1621 aataggcatc atagaaaact tccaaactaa gcagggtgat gttcgtattc cagatgtact
     1681 aaagccattt atgagcggaa aggagcttat atgatcaaaa aaatagaagt aaccagtatc
     1741 cacacggaga ccgaaccaga ggtaaccaag tacgtaaaga aaaagattgc caaactagat
     1801 agctatatgc ccaaacacgc tcgtaaaagt gcccgtgcag aagtccgttt aaaagaagaa
     1861 aaaattaaaa ctaaaaagca atcgagctgc gaagtcgtgc tgtatgttcc aggcgaaatt
     1921 atcaccgcta aagagaccac cgtcaatatg tacgctgccg tagatattgt agaagaaaag
     1981 ctcaaaagcc aattacgcaa gtataaagag caaacaatta tcacgaaaaa acgcggtgat
     2041 gccaaaatta gaaaaatgtt tggcaaaata gtcgggaaaa gtcgttaaaa atacggtaca
     2101 atagacctag tattttctgt ttttaattcg ggtggtaaga aaggtcgttt agttttttat
     2161 gagtaatttt attaagaaag tccttggcga taatcaaaac ggcgcgctcc gacggatgcg
     2221 gcgcaaagta aaagagatta acaagctaga ggatacatac aaggcaatgc caaagaaaac
     2281 attggcagag caaacacaag ttttacaaaa gcgggcagaa aaagaatcgc ttgataaaat
     2341 tttgccagac gcctttgccg ttgtcaggga agccgctcgt cgtattctaa aacagcgcca
     2401 ctttgacgtg cagcttattg gtggcataac gcttcatgac ggtaacgtcg cagaaatgaa
     2461 gaccggtgaa ggtaagacat tagtagcgac tgcgccagta tatttaaacg cacttactaa
     2521 aaaaggtgtg catgtagtaa cggtaaacga ttacttagca cgacgcgacg ccggttggat
     2581 gggtgagatt tatcatttct taggcttgtc tacggctgtt attatccctg atgaatcgta
     2641 tatttatgat ccagaataca ccaacgaaga tcataccgac ccacgctttc aacacctgcg
     2701 tccatgcact cgccaagaag cctaccaagc cgatatcacc tatggcacaa acaacgaatt
     2761 tggttttgat tatttacgcg acaacatggt aaaagaaacc aaccagttac gccaacggga
     2821 acttaatttt gccatcgtag acgaagttga ctcaatttta attgatgaag cgcgaacgcc
     2881 acttattatt agcgcaccaa gtacgacaag tgggcaatcg tacacgcaat tcgcccgcgt
     2941 tgttgcccag ctaaagccag agcactacgt tgtagatgaa aaagaaaaac aggtagtact
     3001 caccgataaa ggtatagaaa aagtagagaa actactgggc ataaaaaacc tatacatcgg
     3061 tgataacata cgtacgttgt atcacctcga acaagcgttg cgtgcccaaa cgctattctt
     3121 gcgcgacaaa gattacgttg tcaccaaaga tggcgaagtt gtgattgtag atgattttac
     3181 cggccgttta ctgaaaggtc gccgctataa cgaaggcctc caccaagcta tcgaagcaaa
     3241 agaaagtgtt actgttcaag aagaatctat gacgcttgcc acgatatcgt tccaaaatta
     3301 cttccgttta tacaacaaat tgtctggtat gactggtacg gcagctaccg aagcagaaga
     3361 atttcatcag atatacaagc tagaagttat tagcatccca accaataaaa agaacatcag
     3421 aaaagattta ccagatcgta tatataagac aaaagaggcc aaaacacggg caattattaa
     3481 agaagttcaa cggttgcata aaaaaggtca accagtactt attggtaccg ttgccataga
     3541 aaataacgaa gaaattagcg cggccctaaa taaagctaaa atcccacacc aagtacttaa
     3601 tgctaaaaat aatgaaagcg aagctcaaat tgtagccaaa gctggccatg ttggtgcggt
     3661 aacgctcgca accaacattg caggtcgtgg tacagacatc gtcttaggtg acggtgtgcg
     3721 tgaac
//
LOCUS       DAOPLS010000058         3655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_28137_length_3655_cov_3.910278, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000058 DAOPLS010000000
VERSION     DAOPLS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28137_length_3655_cov_3.910278"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1690
                     /gene="secY"
                     /locus_tag="PKV52_01975"
     CDS             212..1690
                     /gene="secY"
                     /locus_tag="PKV52_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPD99013.1"
                     /translation="MNWRIIFKSFGSPDMRKRILAVLGVLIIFRFLAQIPIPLAEPAT
                     LKQVLESAFSQTSGSQLLGFLNILSGGALANFSIMLVGLGPYINASIITQLLTKAIPK
                     MEALNKEGEYGRKKIQQWTRLLTLPLAIVQSIGTIFLVRQTAASFGGLGDLVSQASLS
                     QWVLMVTVLTASAMLLMFLGEQITEQGIGNGISLVITVGILASLPGIIGNLLGSVIQD
                     GASFAIFGKFDFPINSAALWVTLGIVVVTVLVTVFVVYLNEAQRRITVNYAKRLQGNR
                     VYGGVTTTLPLRLITAGVIPIIFALAFLALPEFVGRLLSQNATGAMQQLGENLVTWFQ
                     VPTAASLSSGGWIAWLYPIAYFLLVFMFTFFYTSVVFNAKEISENLQKQGGFIDGVRP
                     GAQTEKYLMSTVNKLTLYGALALGFLAIMPIFGQAILGTDQIAIGGTSILILVSVALE
                     TLRQIESRALMVTYDDYTIDISQDGQKTGGKKRFSLKRKKKL"
     gene            1746..1967
                     /gene="infA"
                     /locus_tag="PKV52_01980"
     CDS             1746..1967
                     /gene="infA"
                     /locus_tag="PKV52_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPD99014.1"
                     /translation="MASTKEVIELTGKIIEALPGTQFRVELENGHQIIANLAGKMRKN
                     YIRLVPGDSVKVELTPYDLSKGRITYRLS"
     gene            2017..2133
                     /gene="rpmJ"
                     /locus_tag="PKV52_01985"
     CDS             2017..2133
                     /gene="rpmJ"
                     /locus_tag="PKV52_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPD99015.1"
                     /translation="MKVRASVKKISPDDILVRRKGRLYVINKNKPKHKQRQG"
     gene            2137..2523
                     /gene="rpsM"
                     /locus_tag="PKV52_01990"
     CDS             2137..2523
                     /gene="rpsM"
                     /locus_tag="PKV52_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPD99016.1"
                     /translation="MARLSGVTIPTEKQIGVALTYIHGIGPKSAQDILAKAKVDTTTR
                     VKNLTDAETTRIQDVINESYLVEGELQRTVVSNIKRLKEIKSYRGLRHSANLPSRGQR
                     TKTNARTRRGKKTTVGGTAKKVASKT"
     gene            2545..2940
                     /gene="rpsK"
                     /locus_tag="PKV52_01995"
     CDS             2545..2940
                     /gene="rpsK"
                     /locus_tag="PKV52_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPD99017.1"
                     /translation="MAELSTAAKKKKGKRSIPHGQVHISATFNNTIVSVTDSKGGLLT
                     AASAGACGFRGSKKGTAYAAQVAAEKAIVAAKQLHGLGKADVYVKGIGQGRDAAVRAL
                     SANDVAVETIVDVTGVPHGGCRPRKARRV"
     gene            2949..3566
                     /gene="rpsD"
                     /locus_tag="PKV52_02000"
     CDS             2949..3566
                     /gene="rpsD"
                     /locus_tag="PKV52_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPD99018.1"
                     /translation="MARDTQSITKMSRREGYALHPKAVKHMVKRSTIPGQHGRTNRRS
                     NPSQYALQLREKQKVKRLYGLLERQFSNLMREASRKTGQSGTVLLQFLEQRADNVVYR
                     AGFANSRRAARQLVSHGHFMLNGRRVDIPSIRLKSGDVLTVRPKSTSTEYFKHLDDIT
                     SQNDVVVSWIKVDRKKLTVTVTGLPPREEAEPEINEQLIVEYYSR"
BASE COUNT         1123 a          751 c          813 g          968 t
ORIGIN      
        1 cccacaaacc cacccatgat gctaataaac ccaagtggat cacttccaaa caacactacc
       61 ggaaggtatg cccctaatat actgccaatg gtgataccgc cgtaaatata tagcttggtc
      121 atatttttaa gtatacgtta aattattatg ttacaatact acgtgataat gaaaaccaaa
      181 tatagagagg tgtcttaaaa agtattttct gatgaattgg cgaataattt tcaaatcatt
      241 tggtagtcca gatatgcgca agcgtattct tgcagtgctt ggtgttctta ttatatttag
      301 gtttttggca caaattccta taccacttgc agagcccgct acattaaaac aagtattaga
      361 aagtgctttc agccaaacgt caggttcgca actgcttggc tttttaaata ttcttagcgg
      421 tggcgcactg gctaactttt ctatcatgct cgttgggctg ggcccataca ttaacgcgag
      481 tattattacg caacttctta ctaaggcgat tccaaaaatg gaagcgctca ataaagaagg
      541 agaatacggc cgaaagaaaa tacagcaatg gacacgcttg cttacattac cactggcgat
      601 tgtacaatct ataggaacaa tatttttggt acgacaaaca gcagccagct ttggtggttt
      661 gggcgatctt gtttcacaag ccagccttag ccagtgggta cttatggtaa cagtactaac
      721 agcttcggca atgctcctta tgttccttgg tgaacaaatt actgaacaag gtataggtaa
      781 cggtatttca ctagtaatta ccgtgggtat tcttgcatcc ttacctggta ttattggtaa
      841 cttgctgggt tccgtcatac aagatggcgc tagttttgcg atatttggta aatttgattt
      901 tccaataaat agtgccgcac tgtgggtgac gctgggaatt gtagtggtta ccgttctcgt
      961 gactgtattt gttgtctatc taaacgaagc tcaacggcgc attaccgtaa attacgcgaa
     1021 acgcttacaa ggcaaccgtg tgtacggcgg tgtaacaacg acgttaccac tacgccttat
     1081 tactgcaggt gttatcccta taatctttgc tctcgcattt ttggctctac cagaattcgt
     1141 cggtagactg ctaagccaaa acgctactgg cgccatgcag caattaggcg agaatcttgt
     1201 tacctggttc caggtgccta ctgcagcgag tttgtctagc ggaggctgga tagcgtggct
     1261 atacccaatt gcttatttct tgctggtgtt tatgtttaca ttcttttata catctgtcgt
     1321 gtttaacgcc aaagaaatat cagaaaatct ccaaaaacaa ggtggtttta ttgatggtgt
     1381 acgcccagga gcgcaaacag aaaaatatct tatgagtacc gttaacaagc ttaccctgta
     1441 tggagcttta gcacttgggt tcttagcgat tatgccaatt tttggacaag ctatactagg
     1501 tacagatcaa attgcaattg gtggtacgag tatcctcatc ttggtatcag ttgcactaga
     1561 aacgctccgc caaatagaat cacgcgccct gatggtaacc tacgacgatt acacgataga
     1621 tattagccaa gatggccaaa aaactggtgg taaaaaacga ttttcgctta aacgtaaaaa
     1681 gaagttgtaa ttttctcttt acaaacagtg gataagtgta gtaaactaga ctactgttgt
     1741 aacttatggc aagcacaaaa gaagtaatcg aactcacagg caaaataatt gaggccctcc
     1801 caggcactca atttagagta gagctcgaaa acggccatca gattatagct aatttggccg
     1861 ggaaaatgag gaagaattat attcgccttg tacccggtga cagcgttaag gtagaattga
     1921 ctccttacga tctctctaag ggtcggataa cctatcgctt atcgtaatat taaactaagc
     1981 agtgtgtcac cacagcatta aggagtgatg atccgcatga aagtgcgtgc aagtgtaaag
     2041 aaaatcagtc ccgatgacat tttggtacgt cgtaaaggcc gcctgtatgt tatcaataaa
     2101 aacaagccaa agcacaagca gaggcaaggg tagtctatgg cacgattaag tggtgtaact
     2161 attccaactg aaaagcaaat aggagttgca ttgacgtata tccacggaat tggacctaag
     2221 tcagcgcaag atatccttgc aaaagccaag gtcgatacaa cgactcgcgt caaaaattta
     2281 acagacgctg agacgacacg aattcaagat gttattaacg aaagctacct tgttgaaggt
     2341 gagttgcaac gtacagttgt ttctaatatc aaacgattaa aagagattaa atcataccgt
     2401 ggcttacgcc atagtgctaa tctccctagt cgtgggcaac gcactaaaac aaatgcacgt
     2461 actcgtcgtg gtaaaaagac tactgtcggt ggtactgcaa aaaaggtagc atctaaaacg
     2521 taatatacgt aggagttaat atatatggct gaattatcga ctgctgccaa gaagaagaag
     2581 ggcaaacgaa gcatcccgca cggtcaagtg catatcagtg ctacgtttaa taatactatt
     2641 gtatctgtca cagattctaa gggcggtttg ttaacggctg caagcgcagg agcttgtggt
     2701 tttagaggaa gtaaaaaagg cactgcctat gctgcacaag tagcagcaga aaaagcgatt
     2761 gtcgctgcaa agcagcttca tggtttaggc aaagctgatg tatatgtaaa aggcatagga
     2821 caaggacgtg atgccgcggt gcgtgcttta tctgcaaatg atgttgcagt agaaaccatc
     2881 gtagatgtaa caggtgttcc acatggcggt tgtcgtccac gtaaggccag gagagtatag
     2941 gtgtaattat ggcaagagat actcaatcaa tcacaaaaat gagtcgtcgt gaaggctacg
     3001 cactccaccc aaaagcggta aagcacatgg tcaagcgaag tacgattcct ggtcaacacg
     3061 gtcgcactaa ccggcgttct aacccaagtc aatacgcact acaactgcgc gaaaagcaaa
     3121 aagtaaagcg tctctacggg ttattggaac gacaattttc aaatctaatg cgcgaagcta
     3181 gccgtaaaac cggccagagt ggtacagtgt tactacaatt tttagaacag cgtgcagata
     3241 acgttgtcta tcgggcaggc tttgccaata gccgtagagc tgccagacaa cttgtgagcc
     3301 acggacactt tatgctaaat ggccgcaggg tagacattcc atctattcgt cttaaaagcg
     3361 gtgacgtact caccgtgcgt cccaaaagta cgtcaactga atatttcaag cacctagatg
     3421 acattacaag tcaaaatgac gtcgtggtga gttggatcaa agttgatcgc aagaaactta
     3481 ctgtaaccgt aactggtctt cctccccgcg aagaagctga gccagaaatt aatgagcaat
     3541 taatagttga gtattactca cgataaggag aggacaacaa ggtatgtcta aagttattca
     3601 cacaccatca ttggctaaca tcgatgagca cagcccaact agtgcaacgt tcgtt
//
LOCUS       DAOPLS010000059         3645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_28231_length_3645_cov_5.089136, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000059 DAOPLS010000000
VERSION     DAOPLS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28231_length_3645_cov_5.089136"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1145)
                     /gene="dnaJ"
                     /locus_tag="PKV52_02005"
     CDS             complement(39..1145)
                     /gene="dnaJ"
                     /locus_tag="PKV52_02005"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPD99019.1"
                     /translation="MTKRDYYEVLGVTKDASADEIKKAFRRMAVQHHPDKEGGDETKF
                     KELNEAYEVLKDTEKRKRYDQFGHAGVGGASGQGFGGGAYDFSGGGFNFNGQNINFDF
                     GDLGDMFGSFFGGQQRTRTRRGRDIEVAIELTFEEAVFGVEKSIKLNIESDCEHCKGK
                     GAEPGYELKTCKTCNGSGQVVTVTRTILGNIQQPQTCPDCHGTGKVPEKACSVCGGRG
                     VKRKEQKIDLKIPAGIDDGATIRLREYGEAIQDGQKGDLFVHIRVKPHKKFTREGDLI
                     LSHETVDMVTAALGGVLKVETVDGEVTMKIPAGTQNGDDFKLSGHGVPHLKKDTRGPH
                     IVTLDVKTPTKLSKKQKELLQEFQSASGKRSIFN"
     gene            complement(1138..1422)
                     /locus_tag="PKV52_02010"
     CDS             complement(1138..1422)
                     /locus_tag="PKV52_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HPD99020.1"
                     /translation="MSHVQSTVQRNKRIELYALIAALVQPLLTIPQIAHIYSNQSAQD
                     VSLATWLGYLFFGIVFLVYGFVFKLRPIFYGQLIWVTMQTITVIGILIYD"
     gene            complement(1422..3311)
                     /gene="dnaK"
                     /locus_tag="PKV52_02015"
     CDS             complement(1422..3311)
                     /gene="dnaK"
                     /locus_tag="PKV52_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPD99021.1"
                     /translation="MGKIIGIDLGTTNSAMAVMEGGKPEIIANAEGNRTTPSVVAINK
                     GGERLVGQTARRQAVTNPANTVYEMKRLIGRRWTDKEVQRDIKLMGYEMVKGSDGSVK
                     VKMGKKEFSPEELSAMVLGKLKADAESFLGQSVSEAVITVPAYFDDAQRQATKDAGKI
                     AGLEVKRIINEPTAAALAYGLEKKSTDEKIAVYDLGGGTFDVSILELGDGVFEVKSTN
                     GDTHLGGADFDRAIVNYLLDEFKKESGVDVTDDKAAMQRLRDEAEKAKIELSSSNEVD
                     INLPFLTATADGPVHFETKLTRAKLESLVADLVSRTAGPCEKALKDAGLKASDINAVV
                     LVGGMTRMPAVQAKVKDIFGKEPMKGVNPDEVVAIGAAIQGGVLAGDVKDVLLLDVTP
                     LSLGIETLGGVTTKLIPRNTTVPTSKSETFSTASDGQPQVEIHVLQGEREMSADNKSL
                     GKFVLDGIAPAPRGVPQIEVTFNIDANGILHVTAKDKGTGKEQSITIQNSGNLSKEDV
                     EKAAKEAEANAEADKKKREAIDAKNSLENGIYQAEKLKKDNDEKLSDDDKKELDAIIE
                     AAKKSLESEDKDTLEAASKELTEKLMPIGAKLYEAAAPEDENKADGPAEGEVVDEKDS
                     DKKEK"
     gene            complement(3414..>3645)
                     /locus_tag="PKV52_02020"
     CDS             complement(3414..>3645)
                     /locus_tag="PKV52_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99022.1"
                     /translation="PQYKQSLESLIAAFAWALGKLDMPPTTSWDTLSDSEQDHIQQTL
                     VKDIYKYATYELGLPKQWVDDCFDDVLNMGDV"
BASE COUNT          882 a          856 c          668 g         1239 t
ORIGIN      
        1 ggctgacgag ccgtatgcat agatattata gcaaattact agttaaatat gctacgttta
       61 ccgctagcag actggaattc ttgtagtaat tctttttgct ttttagataa cttagtaggt
      121 gtttttacat ctagtgtaac tatatggggg cctctagtat ctttcttaag atgcggtaca
      181 ccatggccag acaatttaaa atcatcacca ttttgcgtac cagcaggtat cttcatagtc
      241 acctcaccat caacggtttc tactttcaac acaccgccta atgctgctgt taccatgtct
      301 acggtttcgt ggcttagtat caagtcacct tcacgtgtaa actttttgtg cggctttacg
      361 cgtatgtgca caaataaatc tcctttttgg ccatcttgta ttgcttcgcc gtattcgcgc
      421 aagcggatag tcgcaccatc atctattccg gccggaattt taagatctat tttttgttct
      481 ttgcgcttga ctcctctccc accgcataca ctgcaggctt tttctggtac tttgcctgtt
      541 ccatgacaat cggggcacgt ttgtggttgt tgaatattac ctagaatcgt acgtgtaaca
      601 gtgaccactt gcccactgcc attgcaagtt ttacaggttt tgagttcata cccaggctct
      661 gctcctttac ctttgcagtg ctcacaatcg ctttctatgt tgagcttgat tgatttctct
      721 acgccaaaca ccgcttcttc aaaagtgagc tcgatagcta cctcgatatc acgcccacga
      781 cgggtgcgcg tacgctgctg accaccaaaa aagctaccaa acatatcgcc taagtcacca
      841 aagtcaaaat tgatattctg gccattaaaa ttaaaaccgc cgccgctaaa gtcgtaggca
      901 ccaccgccaa atccttggcc actagcgcct ccaacacccg catgtccaaa ctggtcatat
      961 cgtttgcgtt tttcggtgtc ttttaatact tcataggctt cgttaagttc tttgaacttg
     1021 gtttcgtcgc caccttcttt atcggggtga tgttgtacag ccatacgccg aaatgctttt
     1081 ttaatttcgt cggccgaagc atcttttgtg acacccaaca cttcataata atcacgttta
     1141 gtcataaatt agtatcccta tcactgttat tgtttgcatg gttacccaaa ttagttgtcc
     1201 ataaaatatg ggccgcaact taaagacaaa accatacact aaaaatacaa taccgaaaaa
     1261 taaatacccc agccacgttg ctagtgatac atcttgagca ctctgattac tataaatatg
     1321 tgcaatttga ggaatagtta aaagtggttg cacaagcgca gcaataagcg catatagttc
     1381 tatacgctta ttgcgttgta cggtagattg tacgtggctc actatttctc tttcttgtcg
     1441 gaatcttttt cgtcaactac ttcgccttca gccgggccat ctgccttatt ttcatcttct
     1501 ggagcagctg cttcgtatag ttttgcacca attggcatca gcttttctgt gagctctttt
     1561 gatgctgctt caagtgtatc tttatcttcg ctttcaagtg atttttttgc tgcttcgata
     1621 atagcatcta gttctttttt atcatcgtca gataactttt catcattatc ttttttgagc
     1681 ttttctgctt ggtaaatacc gttttcaaga ctattcttgg catcaattgc ttcacgtttt
     1741 ttcttgtcag cttctgcgtt agcttctgct tctttggcgg ctttttctac atcttcttta
     1801 ctaagattac cgctgttttg aatcgtgata ctttgttctt tgcctgttcc cttatcttta
     1861 gctgtaacat gcaatatacc gttggcgtct atattaaacg taacctcaat ttgtggtaca
     1921 ccacgaggtg ccggggcaat accgtcaagc acaaacttac caaggctttt attgtcggca
     1981 gacatttcac gttcgccttg taatacatga atttcaactt gtggttgacc atcagatgct
     2041 gtgctaaatg tttcactttt gcttgtaggg acagtcgtgt tgcgtggaat aagttttgtg
     2101 gttacaccac caagagtctc tatgcctaga gaaagcgggg taacgtctag gagtagtaca
     2161 tcttttacat cacctgcaag cactccacct tgtattgctg cacctatggc tactacttcg
     2221 tctgggttaa cacctttcat gggctctttg ccaaaaatat ctttcacttt cgcttgaacc
     2281 gcaggcatgc gtgtcatacc tccaaccagt acaactgcat taatatctga agcttttaag
     2341 ccagcgtctt ttagtgcctt ttcacatgga ccagcagtgc gactcactag atccgccacc
     2401 aaactttcta gcttggcacg tgtcaatttg gtttcaaagt gtactggacc atcagcagta
     2461 gctgttaaga atggaagatt aatatcaact tcgttagagc tagaaagttc aattttagct
     2521 ttttctgctt catcacgtaa tctttgcata gctgccttgt cgtctgtaac gtctacgccg
     2581 ctttcttttt taaactcatc aagtaaataa ttcacgatgg ctcggtcaaa atctgcgcct
     2641 ccaaggtggg tgtcaccgtt agtgcttttt acttcaaata caccgtcacc taactcaagg
     2701 atagatacgt caaaggtacc gccacctaaa tcgtaaacgg ctattttttc atcagtagat
     2761 tttttttcta gaccatatgc tagggcagct gcagttggtt cgttaattat acgctttact
     2821 tctagaccag caattttacc agcatctttt gtcgcctgac gctgagcgtc gtcaaaataa
     2881 gccggcacag taatgactgc ttctgataca gattgcccaa gaaaactctc ggcatctgct
     2941 tttaattttc ctaagaccat agcagataat tcttctgggc taaattcttt tttacccatt
     3001 tttaccttaa cactaccgtc agagcctttt accatctcgt agcccatcaa ttttatgtct
     3061 cgttgtactt ctttatctgt ccatctacgg ccaatcagtc gtttcatttc atataccgtg
     3121 ttagctggat ttgtaacagc ttgcctacga gcggtttggc ccacaagcct ttcgccgcct
     3181 ttatttatgg cgacaacact cggtgtggtt cggtttccct ctgcattggc gattatttct
     3241 ggcttaccac cctccataac agccatagca ctatttgtcg ttcctaagtc tattccaatg
     3301 atttttccca ttattatagg ttcctttcta aattagcagt aaatgtgatt gattactctt
     3361 gtttatcata cagagtttag cactcatgtg tcaagagtgc taattgttgt gttttataca
     3421 tctcccatat ttaatacatc gtcaaaacag tcgtcgaccc attgcttagg aagacctaat
     3481 tcataggttg cgtatttata tatatcttta acaagtgttt gctgaatatg gtcttgttca
     3541 gaatcactca aagtatccca ggatgtagtt ggaggcatgt ctagtttgcc aagcgcccat
     3601 gcaaaagctg caatgagcga ttctagactt tgtttatact gtggg
//
LOCUS       DAOPLS010000060         3604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_28713_length_3604_cov_4.901381, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000060 DAOPLS010000000
VERSION     DAOPLS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28713_length_3604_cov_4.901381"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /gene="tyrS"
                     /locus_tag="PKV52_02025"
     CDS             complement(<1..446)
                     /gene="tyrS"
                     /locus_tag="PKV52_02025"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPD99023.1"
                     /translation="MTLSEELTWRGFVNQTTYPDISVLDKDPITFYWGVDPSAKSMTV
                     GNFALAMMVLHFVRHGHKPILLVGGATGLIGDPDGKATERDLKTAEELQEYIEGIRNQ
                     YKSIFKDYDFELVDNYDWFKDIGYMEFLRNIGKHVPMSQMLGREFV"
     gene            572..656
                     /locus_tag="PKV52_02030"
     tRNA            572..656
                     /locus_tag="PKV52_02030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:606..608,aa:Leu,seq:tag)
     gene            complement(721..1290)
                     /locus_tag="PKV52_02035"
     CDS             complement(721..1290)
                     /locus_tag="PKV52_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPD99024.1"
                     /translation="MRIIAGAFGGRMLQSPHNNITHPMSEKMRGALFNALGDVTGLSF
                     FDPFAGTGACSLEAISRGAAHATLVEANTDSFKTILVNAKDLGVLDKLTVKRGNCAGW
                     SGNNKSVLFDIAIADPPYKPKDIDIALAFRMARHVRQGGIFVLSAPPAQAETVALRAK
                     QNPQISLLSHKTHGDGSLYFYRQNRTAVL"
     gene            complement(1333..1746)
                     /locus_tag="PKV52_02040"
     CDS             complement(1333..1746)
                     /locus_tag="PKV52_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99025.1"
                     /translation="MNSASKQSPEMPPNPNVFRRVIGTISSIGNLAHKEAITDIPRQA
                     AVAVESAANQFAGMGLEALERTLIDTPQEVETWGREKIISFVHMFIETANTAEGNGKY
                     GKIKPRESDEECVAFFGKLRTMVVMNAIYRKNSKN"
     gene            complement(1755..2384)
                     /locus_tag="PKV52_02045"
     CDS             complement(1755..2384)
                     /locus_tag="PKV52_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99026.1"
                     /translation="MRVNMGNSPEAKQPRIDLKTITGLFSYALAAQFGAERAEDAAQA
                     ALRSAERMARKAERTALQAQESLEEAFDVLRDALQDAHARRNIDAIPRIFQDYIDVVM
                     SAQLACIPPEHPDYDAITAEIAATRERLFANYERLFPENAATCMTIMQDPLPPINIQS
                     GKLTPPTIATTSAQNPTNPGITTMHIDPYDNRTYIINLCSVVVIATQEN"
     gene            complement(2464..3285)
                     /locus_tag="PKV52_02050"
     CDS             complement(2464..3285)
                     /locus_tag="PKV52_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99027.1"
                     /translation="MYSGSTLTNVSGNVIGAHQKIDRAARHALKDLLLDDSKFPSKKM
                     LLHFEGKNGPDSAKAKLDGEYAPWHFYDPFDPDDGHLLEQINAHYTTLVEQLKKGAIE
                     RSAFEASWLAHAIVDGLTPAHHYPFEQELERLRGEGKETRTTVAKKVFIHGETKREAL
                     HKNWEMWGAKGLFTTHALFELGAAMIFAPMGKYIGRPTHYDLKTVQHLGLEEYFKRVA
                     REVAMLNMYEDFYKRGWTPRLTKTARKELAPRMGRTVTVAWFMAAKQAGLVSSEA"
     gene            complement(3286..>3604)
                     /locus_tag="PKV52_02055"
     CDS             complement(3286..>3604)
                     /locus_tag="PKV52_02055"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99028.1"
                     /translation="GVHSGTCYLVMSDSNKPSKRMQAIANTSDGFELAEYDLELRGAG
                     AIYGTMQHGALDLRYVQLTDYALISEVRGAVTAFTKKGDFMVKYPQLADKITKTLQLT
                     YLN"
BASE COUNT          898 a          771 c          756 g         1179 t
ORIGIN      
        1 tttacaaatt cgcggccaag catctggctc attggtacgt gtttgccaat gtttcgtaaa
       61 aactccatgt agcctatgtc tttaaaccaa tcgtaattat ctaccaattc aaaatcataa
      121 tctttaaata tgcttttata ctggttacgt ataccttcta tgtattcctg cagctcttct
      181 gctgttttta gatcacgttc ggttgcttta ccatctgggt caccaatgag accagttgca
      241 ccaccaacaa gcaaaatagg cttatgccca tgcctgacaa aatgcaaaac catcattgct
      301 aatgcaaaat taccaaccgt catacttttt gcacttgggt ctacacccca gtaaaaagta
      361 attggatctt tatctaacac agagatgtct ggatatgttg tttggttaac aaaaccgcgc
      421 catgtaagtt cttctgagag agtcatgcaa atattatgtc cttaggttaa tgctttaagg
      481 atattttacc atatcttatg agctacatat ttattgctct atctgtaaat attgttataa
      541 ttatgtaagc aatttcatgt tagcacgata tgcgcgagtg gtggaattgg tagacacgct
      601 agtcttagga actagtgccg caaggcgtga aggttcaagt cctttctcgc gcaccaaaat
      661 aaatgcccca agattattgg ggtttttata tagttgatat ttatataaat ttactagtat
      721 ttatagtact gcagtgcggt tttggcggta aaaatacagg ctgccatcgc catgagtttt
      781 gtgtgatagc aagctaattt gtgggttttg tttagcccgc aaagctacgg tttctgcttg
      841 ggctggtggc gcagaaagta caaatatacc accttgcctg acgtgtctcg ccattctaaa
      901 agctagagct atgtctatat cttttggctt atagggcggg tctgctatgg ctatatcaaa
      961 taaaacactt ttattattgc cagaccagcc tgcacaatta ccacgtttta ccgttagttt
     1021 atctaagaca cctaaatctt tggcatttac tagtatggtt ttaaacgaat ctgtgtttgc
     1081 ttctacaagt gtggcgtgtg cagcgccgcg actgattgct tctaggctac aagcacccgt
     1141 gccggcaaat gggtcaaaaa aactaagccc tgttacgtcg ccaagtgcat taaataatgc
     1201 tccgcgcatt ttttcgctca tggggtgcgt aatattattg tgtgggcttt gcagcatccg
     1261 gccaccaaat gcaccagcaa ttattctcat agttgttagt atacgctacg caattttttt
     1321 gcaaaattgc tgttaatttt tactattttt cctatatata gcgttcatta ctaccattgt
     1381 gcgaagttta ccaaaaaacg caacgcactc ttcatcactt tcgcgaggtt tgatttttcc
     1441 gtatttgcca ttgccttcgg cagtattggc ggtttctata aacatatgta caaaactaat
     1501 aattttttct ctcccccatg tctctacttc ttgaggagtg tctatgagtg tgcgttcgag
     1561 tgcttctagc cccataccag caaactggtt tgcagccgat tctacagcta cggctgcttg
     1621 tcttggtatg tcagtaatgg cttctttatg tgctaagttg ccaatactgc ttattgtgcc
     1681 gataactcta cggaaaacat tggggttggg tggcatttct ggcgattgtt ttgaagcgct
     1741 attcatattt ttagttagtt ttcttgggta gcaattacta caacagaaca taaattaatt
     1801 atataagttc gattatcgta tgggtctatg tgcatagtag tgatacctgg gttagtgggg
     1861 ttttgtgcac tcgtggttgc tatagtaggt ggggttagtt tgccagattg gatgttgatt
     1921 ggagggaggg gatcttgcat tattgtcata cacgtggctg cattttcagg aaacaatcgt
     1981 tcgtaattcg caaaaagccg ttctcttgtg gctgctattt cggcagttat ggcatcataa
     2041 tctggatgct ctggtggaat acaagcaagt tgagcactca tgacaacatc tatatagtct
     2101 tgaaaaattc taggtatagc atctatgtta cgtctggcgt gtgcatcttg taatgcatca
     2161 cgtaaaacat caaaggcttc ttctagactt tcttgcgctt gcaacgcggt acgttctgcc
     2221 ttacgagcca tacgttcagc tgatcgcagc gcggcttgtg ctgcatcctc agctctttcc
     2281 gctccaaatt gcgcagctag tgcatagcta aaaagccctg ttattgtttt taaatctatt
     2341 cttggctgtt ttgcctctgg gctattgccc atgtttacac gcattggtgt tgctcctatt
     2401 gataatcgtt gaccattatg acagagattc gcatatttgc aagctcaagc ataatagatt
     2461 tatttatgct tcgctactga caagccctgc ttgtttggca gccataaacc acgctaccgt
     2521 aacggtgcgc cccatgcgcg gggcaagttc tttgcgagct gtttttgtta aacgtggtgt
     2581 ccagcctcgt ttgtaaaaat cttcatacat gttaagcata gcgacttctc tagcaacgcg
     2641 tttaaaatat tcttctagcc ctaggtgttg caccgttttt agatcataat gtgttggcct
     2701 gccaatatat ttacccattg gggcaaatat catcgctgcc ccgagttcaa acagcgcgtg
     2761 agtagtgaat aagccttttg cgccccacat ttcccagttt ttgtgcaaag cttcgcgttt
     2821 tgtctcacca tgaataaata ctttttttgc tacggttgtg cgggtttctt tgccttcacc
     2881 ccgtagacgc tccagctctt gttcaaatgg gtaatgatgc gcaggtgtta gcccatctac
     2941 aatagcgtgc gcaagccaac tagcttcgaa cgccgaacgc tcaattgcac ctttttttag
     3001 ttgctctacg agggtggtat agtgtgcgtt gatttgctct aacaggtggc cgtcgtccgg
     3061 atcaaacggg tcataaaaat gccacggagc gtattcacca tctagctttg cttttgcact
     3121 atcgggaccg tttttaccct cgaagtgtaa aagcattttt ttactaggaa atttgctatc
     3181 gtctagcaat agatctttta gtgcatggcg agcggcacgg tcaatctttt gatgggcacc
     3241 aataacgttg cccgacacat tggtaagtgt tgaaccagaa tacatttaat ttaaatacgt
     3301 caattgcaga gtctttgtaa ttttatctgc gagctgagga tattttacca taaaatcgcc
     3361 ctttttggta aaagccgtga ctgccccgcg aacttctgaa attagtgcat agtcggttaa
     3421 ttgtacgtaa cgcaaatcta gtgcgccatg ctgcattgtg ccatatatag caccagcgcc
     3481 acgtagctct agatcatatt ctgcaagctc aaacccgtcg cttgtatttg ctatcgcctg
     3541 catgcgcttg gatggtttat tagaatcgct cataaccaaa tagcaagtac cagaatgtac
     3601 cccg
//
LOCUS       DAOPLS010000061         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_28786_length_3598_cov_3.898391, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000061 DAOPLS010000000
VERSION     DAOPLS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28786_length_3598_cov_3.898391"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..1294
                     /locus_tag="PKV52_02060"
     CDS             482..1294
                     /locus_tag="PKV52_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPD99029.1"
                     /translation="MILAIDIGGSKTLIAPCDEQGNPVQEIKFPTPQKYSDFKKELAA
                     NVVAITTDYNLVVAAVPGKLDRQNGIALAFGNLPWKNVPIRADIAKITGKKTLIENDA
                     NLAGLSEANRIKPLPHVALYITISTGIGTGVITDGVLDPDFFDAEGGNMLLEHEGKLQ
                     IWEKFASGRAIVAKYGKRASEINDPKAWQDIAKLFAIGIVDLTCVLDPDIIIIGGGVG
                     THFKKYGAYLQSYIKEITPVLVATPKIVPAQAAEEAVVYGCAVLAKQYAQRS"
     gene            1380..1748
                     /locus_tag="PKV52_02065"
     CDS             1380..1748
                     /locus_tag="PKV52_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99030.1"
                     /translation="MTYHITNTNDTQSAWALIHEVAHASLNHIDYKSDVDLLRHEVAA
                     WQEAKTMALELGVPIDEDHIQDCLDTYRDWLHKRATCPSCTVVGLQRSDGTYGCFNCQ
                     TSWKVPSSPLCRVSRTIVTS"
     gene            2763..2960
                     /locus_tag="PKV52_02070"
     CDS             2763..2960
                     /locus_tag="PKV52_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HPD99031.1"
                     /translation="MAGTITGGKSAAATNKAKYGKDFYARIGAMGGKKGTTGGFAANR
                     ELARTAGAKGGRISRRTKATK"
     gene            complement(3318..>3598)
                     /locus_tag="PKV52_02075"
     CDS             complement(3318..>3598)
                     /locus_tag="PKV52_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99032.1"
                     /translation="NNIAAPQVTIEGNDTAGTITIVAGANTTAGDLAEIAFNAQFDIA
                     PRAILSAKNANTASLQIFNSTQQNTLIIQTLNAPITGQTYTFDYIIVQ"
BASE COUNT         1055 a          683 c          786 g         1074 t
ORIGIN      
        1 gagtgttgct accttttagc ttacttttgt cgcattttta gtgggaattt tccggctttc
       61 ttgttgttta tttgccattc atctatcagg agtctataaa gccaactgcg ccccagggcg
      121 cataaaaaga aaatagttca tttaccatcc gtcagctgac ggatggctgg gtctgggcat
      181 atcctcctcg tacagattag gatgatgtaa aacgtatcat ggctaagaca aaaaggtgtt
      241 gaagccggct attcattgat aaataagaaa aagccggaaa gttctttttg tgccatgtgg
      301 gttttactat cagcctagta agatgaggtg gctgcccagc gcttttgtta caacttttgg
      361 cggtaaaagt tgtacgttac ataaataaag tagaataagc tatttgttta cttttcttgc
      421 tggatgagaa aagtaagtat gtataggatt ctttgtgtag tacgtgtggt aagataaaag
      481 aatgatatta gcaatagaca ttggggggtc aaaaacactc attgccccat gcgacgaaca
      541 aggtaatcca gtacaagaaa ttaagtttcc tacgccacaa aaatacagcg attttaaaaa
      601 agaattggct gctaacgttg tggcaataac aactgattat aatctggtag tggctgctgt
      661 acctggcaag ttagacaggc aaaatggcat tgcccttgcc tttggcaatt tgccatggaa
      721 gaacgtaccc attcgggcag atatcgccaa aattactggc aaaaaaacac tcatagaaaa
      781 cgatgcaaac ttggctggtc tttcagaagc aaaccgtata aaaccactac cacacgtcgc
      841 gctgtacata actattagta ctggtatcgg aaccggcgtc attacagacg gtgtgctaga
      901 ccctgacttt tttgacgcag aaggtggcaa tatgctgctg gaacatgaag gcaaattaca
      961 aatatgggaa aagttcgcaa gcggcagagc cattgttgca aaatatggca aacgtgcatc
     1021 agaaataaat gacccaaaag cgtggcaaga catagccaag ctatttgcca ttggtatcgt
     1081 agatcttaca tgcgttcttg acccagatat cattattata ggcggtggcg taggtactca
     1141 ttttaaaaaa tatggtgcct atttacagag ttacattaaa gaaataacac cggtgttagt
     1201 agccacacca aaaatcgtgc ctgcacaagc agcagaagaa gctgtcgtgt atgggtgtgc
     1261 ggtacttgca aaacagtatg cccagcgtag ctaatgtctt atcggtccta aaaacacagt
     1321 accctaacgt gaattttgta aagggcaaca agtttgcgtg gtcgccaagt aataaaactg
     1381 tgacctacca tataaccaat acaaatgata cgcagtctgc ttgggcactt atacacgagg
     1441 tcgcccacgc tagccttaac cacatagact ataaaagtga tgttgatctt ttacgacatg
     1501 aggtcgcagc gtggcaagag gcaaagacca tggcgcttga gcttggtgtt cctatagatg
     1561 aagaccacat tcaagattgc ctagatacat atcgcgactg gttacacaaa cgcgcaacct
     1621 gcccttcttg tactgttgta gggttgcagc gtagcgacgg tacgtacggc tgttttaatt
     1681 gccaaaccag ctggaaggtc ccatcttcgc cactgtgccg cgtgtcccgc acaattgtca
     1741 ccagttaatt atgaatagtt agtatggaat catctagcgt catttaataa tttggtagat
     1801 ttcacttatt aatacttgtt taagcaacaa aattgcaaca aaactgtcga gaggaatact
     1861 agtactctta tctatttttg taatcctact ttgcatgcta tagtaggcaa accaccgcaa
     1921 tgtattctct ttctttgtct ggtcaaaata ccctatccaa ctttatgcgc taaagttggg
     1981 caaagcaccg gggctgcgtt ttacctatgc gtgcactatg gataactata acaactgcaa
     2041 aaagaatccg tcagtagacg gacaggctcc accctagctt tattacttca gataatggtg
     2101 gcttaacacc tttttgctta taagttgtta ttgttaccga ttgcatcctg ggtaaagccg
     2161 atgccccgga cccccatgcg ccacttgtga cgcttagaag aataccttct ttgtttgtgc
     2221 cgataggcac aaagggcatc ttctttgcat agtgatatag atgccgtatt tataacatgt
     2281 ccaaggcaaa aagatgtttg agcaatttat tctgaagtaa aaagcaaatt gttagttctt
     2341 tttgcgatat gtcagaaata ctggcaagta cgtcgttgca aagaagcgcc gaggcttttt
     2401 gttacttttt agccggcaga aaaagtaagc aaatactttt gtgccaatgt actcatttat
     2461 caatagataa gaaatgcagt gctacgccca gcgcttttac gcaacttttg gcggtaaaag
     2521 ttgcaaaaga tcagtctaaa tatgctattt ttatccgcta gctgacagac tgttagaaaa
     2581 agtaagtaat aattcttgga aagcagagta gaattaaatg catgtttgaa ataattggtt
     2641 ttaacataat tatattgact ttttagcaat agcatgttag attagtgttt atagagggtg
     2701 ttctctgtct atgcaggcag atggcactct ttttttattt agtaatcaag ggaggatact
     2761 aaatggcagg tacaataaca ggcggtaaaa gtgccgctgc gacaaacaag gctaaatacg
     2821 gcaaagattt ttatgcacgt attggcgcaa tgggcggcaa aaaaggcaca actggtggct
     2881 ttgcagccaa ccgtgagctt gctcgaacag caggcgcaaa aggtggccgt attagccgcc
     2941 gcacaaaagc tactaaatag taatttacat tacgtattga atagctagta aaaaacagga
     3001 tctttttagg atcctgtttt tgcattgtaa gcactatgtg ggtactaatt ttctggcaaa
     3061 agaaaaatgc aactaccagc tgcgagatta gtagttgcat ttaacttttt gaaatgtttt
     3121 tgtttgtact tacattattg cactgtaatg cttatggtgc aacacttttg cacaaaaata
     3181 gtgttcactt ttttttaccc ctgtaatttc tgtggataag tttggtaaaa ctacgcacca
     3241 tgcaacatat gtttgaatag ctctgccatt tttatactca gctttatgca cacatttcaa
     3301 taatttagta gcctatttta ctgcacaatg atgtagtcaa acgtatatgt ttggccggtt
     3361 attggtgcat taagtgtttg tattattagt gtgttttgct gcgtgctatt aaatatttgt
     3421 aggctagctg tgttggcatt tttggcagat aatatggctc ttggtgctat atcaaactgt
     3481 gcgttaaaag ctatttcggc taagtcgcct gctgtggtgt tggcgccagc aactatggtg
     3541 atggtgccgg cggtgtcgtt accttctatg gttacttgtg gggcagctat gttgtttt
//
LOCUS       DAOPLS010000062         3585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_28938_length_3585_cov_5.117280, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000062 DAOPLS010000000
VERSION     DAOPLS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28938_length_3585_cov_5.117280"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..364
                     /locus_tag="PKV52_02080"
     CDS             119..364
                     /locus_tag="PKV52_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99033.1"
                     /translation="MTSPESISPEFAARLAEKEQARKRTELKRALALFAFALAAVFFA
                     SQEGLIDNPFATHPDSSVSIEDGPDTSRHTIPTGTER"
     gene            463..1095
                     /locus_tag="PKV52_02085"
     CDS             463..1095
                     /locus_tag="PKV52_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99034.1"
                     /translation="MSHEQVPVRDPKGEHEVDSSWDLDKASKLAPLLLQDKGFRNKMN
                     DLSRDPENLSLKLETDEAAEGLIRFTEMLYDEIPELFENIPDEQKDDVARELYGLARK
                     EAEKTQDAERYEVCAAEIREWHKKKMPMGVAMSLGYGEFLSRREIDFAPDIALQMKNI
                     LNHYEMSDSDKSLAMIALFETLRDEANVASHDAGSAYEELFDKFLPTTEK"
     gene            1254..1754
                     /locus_tag="PKV52_02090"
     CDS             1254..1754
                     /locus_tag="PKV52_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99035.1"
                     /translation="MSEQMPMQSEVNTQLDKKPDTVVDPQKAEIMAYAENPIRSSAIL
                     KREIVDTLEEAAKLNPAKTFNSVNSLADYFVELGSQSEDNPFINWNTEYADAVANRII
                     KAQYGDKTELTVIDVINYFESDAEYLDIDANMEGERASDSFDALQKRMGEIAASRKGK
                     YTRTSL"
     gene            2056..2484
                     /locus_tag="PKV52_02095"
     CDS             2056..2484
                     /locus_tag="PKV52_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99036.1"
                     /translation="MKANKKNELVRNKQKAWYMAHAEKPYQERIIATEQIESMAIDLQ
                     EFHQEDIVVSKKTMQKMMGVGRLALLRYWLRGEPSYYISQCGKTAIISTIDFIYYGQA
                     KELSASEIEYQAEGLVGQLEVWSTTNAKTAWRMHKITSNI"
     gene            complement(2523..3578)
                     /gene="prfA"
                     /locus_tag="PKV52_02100"
     CDS             complement(2523..3578)
                     /gene="prfA"
                     /locus_tag="PKV52_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPD99037.1"
                     /translation="MEQKIHTLRAELAELKGRLNDTAIFSSPEYPKLAKRQNYLESTI
                     ALYDDYIAAQEQLTEAKELLSSGGDLAELAEIEVTELTTKLATLSEALDAALTPKDPN
                     DERDCIIEIRAGAGGDESSLFSADLYRMYVRYGELHSFKIELISESPSESGGFKEIIF
                     AIRGSDAYKTYKYEAGVHRVQRVPVTESQGRIHTSTATVAVLSEAEETDIELNPSDLR
                     VDVYRASGHGGQSVNTTDSAVRITHVPTGLVVTCQDEKSQIKNKDKAMGVLRARLMQM
                     KIDDEQKQLSDARKKLIGSGDRSEKIRTYNFPQDRITDHRIGYSRSNIPAALAGDIQD
                     LIDELANAERHALQDSA"
BASE COUNT         1151 a          648 c          770 g         1016 t
ORIGIN      
        1 cagtgtagaa cagccacatg ttatgtgttg caaagctgtt gagtcattac gcaaagcttg
       61 actaattagc actagcatga tactataggt ttataaagta acaataaata gataaataat
      121 gacatcacca gaatccatct caccagaatt tgccgccaga ttggcagaaa aagaacaagc
      181 aagaaaacgt acagaattaa aaagagcatt ggcgctattt gcgtttgcgc tagcagctgt
      241 cttctttgca tcgcaagagg gacttattga taacccgttt gccacacacc cagattcttc
      301 tgtatctata gaagatggtc cagatacttc acgccacact atacctactg gaacagaacg
      361 ttagtacact gtaaaaattt gacaaaacac tagcattatg ctactatgtt ctataacgac
      421 tgacaaacaa aaactactaa aaaataaaaa aggaataaat taatgagcca cgaacaagta
      481 ccagtacgtg atccaaaagg agaacacgaa gtagatagta gctgggattt agacaaagca
      541 agtaaattag caccactgct attacaagat aaaggatttc gtaataaaat gaatgattta
      601 tctagagatc ctgagaattt atctttaaag ctagaaacag atgaggccgc ggaaggctta
      661 ataagattta cagaaatgct gtatgatgaa atacctgagt tgtttgaaaa tatacctgat
      721 gagcagaaag atgacgtcgc acgagaacta tacggccttg ccagaaaaga ggctgaaaag
      781 acacaagatg cagaacgata cgaagtgtgt gctgcagaaa tacgtgaatg gcataaaaag
      841 aagatgccta tgggcgttgc tatgagtttg ggatatggcg aatttcttag tagaagggaa
      901 atcgattttg cgccagatat tgcacttcaa atgaaaaata ttcttaacca ctacgagatg
      961 tcagattctg ataagtcatt agcaatgatt gctttgtttg aaactcttcg tgacgaagca
     1021 aatgtcgcat cacacgacgc aggctctgcc tatgaagaat tatttgataa attcttacct
     1081 actactgaaa aataagaaga gtaaataagt ttgaagaatt gtataagtca ttagtcttag
     1141 gtgagataac agtaatatac tctgaaaatt tgacaaaaca ctagcattat gctactattg
     1201 atgctaacga ctgacaaaca aaaactaata aaaaataaaa aaggaataaa aaaatgagcg
     1261 aacaaatgcc catgcaaagt gaagtaaata cacaattaga taaaaaacct gatactgtcg
     1321 tagatcctca aaaagctgaa attatggcat atgcagaaaa tcctatcagg tcatctgcga
     1381 tattaaaaag agagatagtt gatacgcttg aagaagcagc taaacttaat ccagctaaga
     1441 cttttaattc tgtaaatagc ctagctgatt actttgtaga attgggttct cagtccgaag
     1501 acaatccttt tataaattgg aatactgaat atgccgatgc tgtggcgaac agaataatca
     1561 aagctcaata tggagataag actgaactta cggttattga tgtaattaat tactttgaat
     1621 ctgatgcaga atacttagac atagatgcta atatggaggg tgagcgtgct tccgattcat
     1681 ttgatgcgtt acaaaaacga atgggtgaaa tagctgctag cagaaaagga aagtatacca
     1741 ggacttcatt ataatagttt agggtactaa gttctaacgc aatacatcat tataacggat
     1801 gggtgaccat ccgttgttaa tttattctta taattcaaat cctaacctct aaaatctagt
     1861 tattttcttg gagggcgtgg cgttcggcgt ttgcgagttc atctataggg tcttgaatat
     1921 caccagccac agtaaatggt atttcacaaa atattaattt ctaagtagtt attaagcaaa
     1981 ctggtcatag tgtttgtggt tgcgtcgcct taagacagta ggatactatt acccttatta
     2041 aatatgaggt taagagtgaa ggccaataaa aaaaatgagt tagtgcgaaa taaacaaaaa
     2101 gcatggtata tggctcatgc cgaaaaacct tatcaggaac ggattattgc aactgaacaa
     2161 atagagagca tggccattga tttacaagaa tttcaccaag aagatatagt agtttcaaaa
     2221 aagacaatgc aaaaaatgat gggtgtaggc aggctcgctt tattaagata ctggctaaga
     2281 ggtgagccgt cgtactatat ttcgcagtgc ggaaaaacag caatcataag taccatagat
     2341 tttatctatt acgggcaagc taaagagctg tcagcttctg aaatagaata tcaggcagaa
     2401 ggtcttgtag gtcagctaga agtgtggtca acaacaaatg ctaaaacagc ttggcgaatg
     2461 cacaaaataa catccaatat ctagtatcca taatctaata tctataatcc aatactaata
     2521 atctatgcgc tgtcttgtaa ggcgtggcgt tcggcgttgg cgagttcgtc aataaggtct
     2581 tgaatatcac cagccagcgc ggcagggata ttgctgcggc tgtagcctat gcggtggtcc
     2641 gtaatacggt cttgcggaaa gttataggtg cggatttttt cgctgcggtc gccgctgcca
     2701 ataagttttt tgcgggcatc gctgagctgt ttttgctcat cgtctatctt catttgcatt
     2761 aaccgtgccc gtagcacgcc cattgcttta tctttgtttt tgatttggga cttttcgtct
     2821 tggcaggtaa caacaaggcc agtaggtacg tgggtaatac gcacagcact atctgttgtg
     2881 tttacacttt ggccgccatg gccgctagct ctatatacat ctacccgtaa gtcactgggg
     2941 ttaagctcta tgtctgtttc ttctgcttct gataacacag ccacagttgc tgtgctggtg
     3001 tgtatgcggc cttggctttc tgtaactggt acgcgttgca cgcggtgtac gccggcttca
     3061 tacttatatg ttttgtaggc atcgctccca cgaatggcaa aaataatttc tttaaaaccg
     3121 ccagattctg atgggctttc gctaatgagc tcgattttaa aactgtggag ttcgccgtag
     3181 cgaacataca tacgatacag atctgcacta aacagtgaag attcatcgcc accggcacct
     3241 gcccgaattt ctataataca gtcgcgttcg tcgtttggat cttttggggt gagggcagcg
     3301 tctagcgctt cacttagggt agcaagtttg gtggtgagtt cggttacttc tatttctgca
     3361 agttcggcaa ggtcgccacc actgcttagc agttcttttg cttctgtaag ctgttcttgg
     3421 gctgcgatgt aatcatcata taaggcgatg gtagattcta aataattttg gcgtttggcc
     3481 agttttgggt actctgggct actaaaaata gcagtgtcat taaggcgacc ttttagttcg
     3541 gcaagttctg cacggagggt atgaattttt tgttccatat ttata
//
LOCUS       DAOPLS010000063         3571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_29098_length_3571_cov_3.899317, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000063 DAOPLS010000000
VERSION     DAOPLS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29098_length_3571_cov_3.899317"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2077
                     /gene="valS"
                     /locus_tag="PKV52_02105"
     CDS             <1..2077
                     /gene="valS"
                     /locus_tag="PKV52_02105"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPD99038.1"
                     /translation="DDGLIYRGERLVNYCTHHRTGFADIEVEYQEKESPLYYLKYGPF
                     ELATTRPETKFGDTAVAVHPEDERYKDLVGKVISVEGVNGPFEIRVVADAMVDRTFGT
                     GVVKITPAHDFNDWEVAQRHNLEAIRVINHDGTMNHRAGKFEGLTVAEARKAVVDALK
                     EKDLLVKVDTHYKNRVGTCYKCGSVIEPMLMDQWFISMQKLAAPAIKSLQNKEITFYP
                     ASKRQQVITYLQSVKDWNISRQIAWGIPIPAFQNINDPDDWIFNTSVDQEVIELNGQH
                     YRRDPDVFDTWFSSGHWPFVTLGYPDSQDYKDFYPYSVMETGQDIFFQWVARMICIGI
                     YVTGKAPFKDVYLHGMVRAQDGKKMSKSLGNVIDPNNELSTYGADAVRVGMIAGRTAG
                     YSAAYAPSKVLAGRNFCNKLWNIARYCETVLGPDFSRENLKASSLADNWIIDRANTVI
                     TDVSKLLDTYRFSEAYDRVYHFVWDDFADWYVEASKAQLNKNVLAYVLEVALKITHPF
                     APFVTETIWQTLSWKQEELLIRASWPTVVKVDEKAATNFTDIKNIVTEIRYISSALDV
                     KNPSLYFTNNSFLKDNADIIARFGRLERVGEVESGRGLHLTTTKHNCWLDIDLHTAKV
                     YVTKLRVAKEDKRALIERLQQRLQNKAYTQKAPKAVVQQTKDQMLHEEELLKKLEAEL
                     TTFETLSQS"
     gene            complement(2468..3334)
                     /locus_tag="PKV52_02110"
     CDS             complement(2468..3334)
                     /locus_tag="PKV52_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPD99039.1"
                     /translation="MSEVGPNQELILAQAIAAARKAAGFTQQELCNTANLSYSTLAKI
                     ERGAIKTPSVFTVATIAAVTGTTVEALTGITTTQAPTAPAKQYQTAKNGVRFVYFDVN
                     GTLVRFFQRAFTQISADTGASADGIEAMFWHYNDDICKGQMPTEEFNSILAKRIGVPS
                     IDWGKYYLANIEPITELKECVAWVADHYKIGLMTNIMPGLLQQMLQTHILPDVAYDAI
                     IDSSEAGYIKPEPEIYQAAQDMAGCEPAEILLIDDSRPNLMAAERLGWHVLWFDDYRP
                     EEGAKRIIEALA"
BASE COUNT         1066 a          765 c          778 g          962 t
ORIGIN      
        1 ggatgatggg ctaatttaca ggggtgaacg tttagtaaat tactgcacac accaccgtac
       61 tggctttgct gatatagaag ttgaatatca agaaaaagaa agcccactat attaccttaa
      121 atatgggcca tttgaactcg ctacaactcg cccagaaaca aagtttggcg atactgctgt
      181 agctgtacat ccagaagacg aacggtataa agaccttgtt ggcaaagtaa tatcggtaga
      241 aggcgtgaat ggaccatttg aaatacgcgt ggttgcagat gcaatggtgg atcgtacttt
      301 tggtactggg gtcgttaaga ttacaccagc acacgacttt aacgactggg aagttgccca
      361 gcggcataac ctagaagcga tacgcgtaat taaccatgac ggcaccatga accaccgcgc
      421 tggcaaattt gaaggcttaa ccgtcgccga agctcgcaaa gcggtcgttg atgcactcaa
      481 agaaaaagac ttgcttgtaa aggtagatac gcactataaa aaccgagttg gtacctgtta
      541 caagtgtggc agtgttatag aaccaatgtt aatggaccag tggtttatta gtatgcaaaa
      601 actagctgca cccgccataa aaagtttaca aaataaagaa attacatttt acccagccag
      661 taaacggcag caagtgatta cctatttaca atctgtcaaa gattggaaca tcagccggca
      721 aatcgcatgg ggtataccta ttcctgcatt ccaaaacatt aacgacccag acgattggat
      781 attcaacaca tcggtagacc aagaagtcat agagttaaat ggccaacatt atcgccgtga
      841 ccctgatgta tttgacacct ggtttagcag cggacattgg ccatttgtca cgcttggcta
      901 tccagacagt caagattaca aagattttta cccctatagt gttatggaaa caggccaaga
      961 tatttttttc caatgggtgg cccgcatgat ttgtatcggc atatatgtaa ctggtaaggc
     1021 accgtttaaa gacgtttacc tgcacggcat ggtacgggca caagacggca aaaaaatgag
     1081 caagagtttg gggaatgtta ttgaccctaa caatgagctg agtacatatg gtgcagatgc
     1141 cgtacgcgtt ggtatgatag ctggtagaac ggctggctat agtgcggcgt atgcacccag
     1201 taaagtgctt gccggccgaa acttttgtaa taaactttgg aacatagcac ggtactgcga
     1261 aaccgtactg gggccagatt tttcacgtga aaacctaaaa gcatcgtcgc ttgcagataa
     1321 ctggattatt gaccgggcaa atacggttat tacagacgtt agcaagttac tagacacgta
     1381 tcgctttagc gaagcgtacg accgcgttta ccactttgtg tgggacgact ttgccgactg
     1441 gtacgtagaa gccagcaaag cgcaacttaa caaaaatgtg ttggcgtacg ttttagaagt
     1501 tgctcttaaa attacgcatc cgtttgcacc atttgtgaca gaaactattt ggcaaacact
     1561 tagttggaaa caagaagaac tattaattcg cgctagctgg cctactgtag tgaaagtaga
     1621 tgaaaaagcg gcaactaatt ttaccgatat taaaaatatt gtgacagaga tacggtatat
     1681 aagtagcgcg ttggacgtta aaaacccaag tttgtacttt acgaataatt cattcctaaa
     1741 agataacgct gatattatcg ccagatttgg gcggctagaa agggtagggg aagtagagtc
     1801 gggcagggga ctacacctta caactaccaa acacaactgt tggctagaca tagatttgca
     1861 taccgcaaaa gtgtacgtca caaaattacg tgtcgccaaa gaagataaac gtgcactcat
     1921 tgaacggttg caacaacgct tacaaaacaa ggcatatacg caaaaagcac caaaagccgt
     1981 tgtgcaacaa actaaagatc aaatgttgca cgaagaagaa ctcttaaaaa aactagaagc
     2041 cgagcttact acttttgaaa ctcttagcca aagctaacat attttaataa gctaaagaaa
     2101 agcccccgat atcgctcggg ggcaggcgtg tttgggggca gtggtcatac catgcccttg
     2161 taatgcagcc tgtgttgctt gcccgtgtaa ataacggctt gatttatgct ggtcacaggt
     2221 aagccgttcc aaacactgcc aaatggcagc atctgcccac agtacgcgtc taccgcgtag
     2281 gttattttcg caccacaact gcagtgcgta gtaccaccaa tatagttcat caaacagcag
     2341 gcggtctcgc tgtttgtatg atacaccagc acccacgttt ctggcattgc tcctcctaat
     2401 gtggaaaaac atagcactgt gctacataaa ctattatata atatttagtc tttttagtct
     2461 agccttctta ggcaagggct tctatgatgc gcttggcgcc ttcttctggg cgataatcat
     2521 caaaccacag tacgtgccac ccaagccgtt ccgcagccat caggttgggc ctgctatcgt
     2581 ctataagtaa tatctctgcc ggttcacagc ctgccatatc ttgagctgct tggtatattt
     2641 ctggttctgg cttaatgtac ccagcttctg atgaatctat aatggcatcg tacgcaacat
     2701 cgggcaaaat atgtgtttgt agcatttgct gtaacaatcc cggcataatg ttagtcataa
     2761 gccctatttt gtaatgatct gctacccacg caacacattc tttgagttcg gtgattggtt
     2821 ctatattggc aagatagtat ttaccccagt ctattgatgg gacaccaatg cgttttgcca
     2881 aaattgagtt aaattcttct gttggcattt ggcccttgca gatgtcgtca ttatagtgcc
     2941 aaaacattgc ctctatacca tctgcagacg caccagtatc ggctgaaatt tgtgtaaaag
     3001 cacgctgaaa aaaacgtacc aatgtaccat tgacgtcaaa gtacacaaac cgaacgccat
     3061 ttttggcagt ttggtactgt tttgcgggtg ctgtaggtgc ttgtgttgtg gtaatacccg
     3121 ttaaagcttc tacggtggta cccgtaacgg cggcaattgt tgctacagta aaaacacttg
     3181 gtgtctttat agcaccacgt tcaatttttg ctaaggtgct atacgacaaa tttgcagtat
     3241 tacaaagttc ctgctgcgta aacccagctg cttttctagc agcagcgatt gcctgcgcta
     3301 atattaattc ttggttagga cccacctctg acattactta ttattctagc acgatacggc
     3361 tagtagtata caaatattgc aaattaatct atattatcgg gcttgtattg caacgaaata
     3421 accgttgggt atgagcaacg gtacacgcat tgacatccac taccaagcca aacacactac
     3481 tcatcctcaa cactttaaat aaatgcttct tttgcataaa acacatatat ttaacacggt
     3541 cgtgttaaat atatgtgttt tatggttgtg t
//
LOCUS       DAOPLS010000064         3562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_29200_length_3562_cov_3.558597, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000064 DAOPLS010000000
VERSION     DAOPLS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29200_length_3562_cov_3.558597"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKV52_02115"
     CDS             complement(<1..882)
                     /locus_tag="PKV52_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HPD99040.1"
                     /translation="MSRLLHRHSPVRIFGISVVLTILISAISIAYNGAQVIPVLFALL
                     IIEITFSFENAIINAKVLTTMSKFWQTMFLTIGIFIAIFGMRVVFPIALVAIAANTSW
                     QTVLDTALNDPVAYSAQLEVAYPSIAAFGGAFLLMLALSFFFDKTKKTLWLTPIESRL
                     HRVSHWYMPSLMTAGVIAFFAALPRNDHPETTIAAGAVGVVTYIVVHGLAEFFTKIQS
                     GHNGKRATTTVRYGFAAFLTFLYLEVLDASFSLDSVIGAFAITNQVLLIAAGLGIGAV
                     WVRSMTVYMVRKGSLATY"
     gene            complement(879..1298)
                     /locus_tag="PKV52_02120"
     CDS             complement(879..1298)
                     /locus_tag="PKV52_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD99041.1"
                     /translation="METKPANGIKILCVEDEHFISELYARALRRAGYDVTTLLSGEDG
                     LRSALKDDYDIILLDLMIPGITGFEVLRELRAKAPNLKAKILITTNLDQEDESREEIE
                     KMADGYLIKAEFTPRQLVEIIDNVASGNDIAPQPTET"
     gene            complement(1300..2832)
                     /locus_tag="PKV52_02125"
     CDS             complement(1300..2832)
                     /locus_tag="PKV52_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPD99042.1"
                     /translation="MIAHLYPLRESIWRKIMFAAIGTSVLAISVYYGVSQILKVDTLM
                     SVYIALGTSVIVGIVLGRVVANEVIKTTDFLTRAIMVALKEDDEVDRPSEQDIGAPSR
                     EFLLKLAERAMDVSQALQQQGQMHDQELVYYRTLVNVLSLPLIVVNTDQQITFANEAA
                     LKYLELPANEVIGQYFYDACNLSFVSETTLEAWLQSCREGAVVENEVWERVRLNLPEN
                     KRKQFDLAAHYRKDDVTGIELALTFFDRTMQYERDDHDLSFVSLAVHELRTPLTIMRG
                     YIEVFEDELSPTLSSEQTAFMHNMAASAQRLSSFVSNILNVARVEENALLLRLKEEPW
                     ADVLQQACKDMQLRAQVHDKNLIIDIDPDLPTIAVDKVSIYEVLNNLLDNAIKYTHTK
                     EDIVIKSYLKDDMIETTVTDRGVGIPSSLIGHVFDKFYRAHQSKNSVGGTGLGLYLCK
                     AIVDAHGGTIWVQSKEGQGSTFGFLLPTYASVADQIDDQDNKEIIRGAHGWIKNHSLY
                     RG"
     gene            complement(2829..>3562)
                     /locus_tag="PKV52_02130"
     CDS             complement(2829..>3562)
                     /locus_tag="PKV52_02130"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HPD99043.1"
                     /translation="TRFMAVYQESQPDKIGPIRSARPYYVDYIAGLDGSYGHVGGSPE
                     ALQDIKSLGIKDLDQFYNANAYTRLSSKSAPHNVYSDFARLDSLNQSKGYTSSKFTGF
                     ARKIDVPQTPTANTVNLILSGPKYNAQFSYDSASNSYLRSQAGAPHKDEPTGKQINPK
                     VVIALVANKGNDPDGYHTNYTTTGSGKVFVFQDGIVSVGTWEKPDRKSSLALKDSFGL
                     PLKLNAGQTWLTLVASADDVRYDVQ"
BASE COUNT          979 a          765 c          731 g         1087 t
ORIGIN      
        1 ataggttgca agcgaccctt tgcgtaccat ataaacggtc atggatctca cccatacagc
       61 cccgataccg agcccagcag ctattagaag gacttgattt gtgatagcaa aagcaccaat
      121 cacactgtct aggctaaagc tcgcgtcgag cacttctagg tataaaaaag tcaaaaatgc
      181 agcaaaacca taacgtacgg tagtagttgc tcgtttgccg ttgtgcccag actgtatttt
      241 tgtaaagaat tcagccaggc catggaccac tatatacgtt acaacgccaa cagcacctgc
      301 tgcaattgtt gtttctgggt ggtcatttct gggcaaagcg gcaaaaaaag caataacgcc
      361 ggctgtcata agactaggca tgtaccaatg gcttacacgg tgcagacgag attctattgg
      421 tgtaagccat agtgtttttt ttgttttgtc aaaaaagaaa cttagggcaa gcattagcaa
      481 aaaagcacca ccaaatgctg ctattgacgg atatgccact tctaattggg cagaatacgc
      541 aacaggatca ttaagagcgg tgtctaagac ggtttgccaa cttgtgttcg ctgctatcgc
      601 tacgagagca atcggaaaca caactcgcat accaaatatt gcaataaaaa tacctattgt
      661 taaaaacatt gtttgccaaa atttagacat cgttgtaagt actttggcgt taataatggc
      721 attttcgaaa ctaaacgtaa tttcaattat taagagagca aacaagacag gaataacctg
      781 cgctccgtta tacgcaatgg atattgctga aattaagata gtgagtacaa cagaaatacc
      841 aaatattctc actggcgaat gacgatgcag tagacggctc atgtttctgt aggctgtggg
      901 gcgatatcgt tgccacttgc tacgttgtca atgatttcaa cgagttgccg aggcgtgaat
      961 tcagctttta tcaaataacc atctgccatt ttttctattt cttcacgaga ttcatcttct
     1021 tgatcaaggt ttgttgtaat gagtatttta gctttaaggt taggagcctt cgcacgtaat
     1081 tcacgcagta cctcaaagcc ggtaatacca ggaatcatga gatctagcaa tataatgtcg
     1141 taatcgtcct tgagtgcaga acgtagacca tcttcgccac ttaataaggt tgtcacatca
     1201 taaccagctc gcctaagtgc ccgtgcatat agttcactaa taaaatgttc gtcttctacg
     1261 cacagtattt taattccgtt tgctggtttc gtttccatgt taccccctat agagcgaatg
     1321 gtttttaatc catccatgcg ctcctcgtat tatttctttg ttatcttggt catcaatttg
     1381 gtcagctact gatgcatagg ttggtagtaa aaagccaaat gtgctgcctt ggccttcttt
     1441 actttggacc catattgtac cgccgtgagc gtctacaatg gccttacaaa gatataagcc
     1501 gagcccagta ccaccgacgc tgttcttgct ctggtgagct cggtaaaact tgtcaaatac
     1561 gtggccgatt aaactagatg gtatacccac acctcggtcg gtaaccgttg tctctatcat
     1621 gtcatcttta aggtatgatt ttattacgat atcttcttta gtgtgcgtgt atttaatcgc
     1681 attatctagc aagttattta atacttcata aatacttact ttatctactg caatcgttgg
     1741 caaatctggg tctatatcta taatgagatt cttatcatgt acttgagctc tcagctgcat
     1801 gtctttgcaa gcttgttgca agacgtcggc ccatggttct tctttaagcc gcaataatag
     1861 ggcattttcc tctacccttg ctacgttaag gatattagaa acaaaagatg ataatcgctg
     1921 agcacttgct gccatgttat gcatgaatgc agtttgttct gaacttagtg tggggctgag
     1981 ttcgtcttca aaaacttcta tgtaacctct cataattgtg agtggtgtgc gtagttcgtg
     2041 aactgctaaa cttacaaaac taagatcatg atcatcacgc tcatattgca tagttctatc
     2101 aaaaaatgtc agagccagct caatacccgt gacatcatct ttacggtagt gtgctgccaa
     2161 gtcaaattgt ttgcgtttat tttcagggag gttaagccgc actcgctccc aaacttcatt
     2221 ttctaccacg gccccttcac ggcaactttg taaccatgct tctagagttg tttcactcac
     2281 aaatgataag ttgcaggcat cgtaaaagta ttgcccaatc acctcattag ctggcagctc
     2341 taaatattta agtgctgctt cattagcgaa agtaatttgt tgatcagtat tgacgacaat
     2401 cagcggtaaa gaaagtacat ttaccaacgt tctgtaataa acaagctctt ggtcatgcat
     2461 ctggccttgt tgttgcaagg cttggctaac atccatcgct ctttctgcga gttttagtaa
     2521 aaactcgcga gacggtgcac ctatgtcctg ctcacttggt ctatctactt cgtcgtcttc
     2581 ttttagagca accataattg ctcttgttaa gaaatcggta gtttttatga cttcattagc
     2641 gacaactctt cccaatacta taccgacaat caccgatgtg ccgagtgcaa tatagacgct
     2701 catgagcgta tcaactttca agatttgaga aacgccatag tagacactaa tagccaggac
     2761 actcgtacca atggctgcaa acataatttt acgccatatg ctttcgcgca atgggtatag
     2821 atgtgcgatc attggacgtc gtatcgaacg tcgtcagcag atgcaacgag cgttagccat
     2881 gtttgaccag cattcaattt taatgggagc ccaaatgaat cttttagtgc gagagatgat
     2941 ttgcgatccg gcttttccca tgttccaaca ctcacaatgc cgtcctggaa gacgaatact
     3001 ttaccagaac ctgtggtggt gtaattggta tgatacccgt caggatcgtt gcctttattg
     3061 gcgactagtg cgataactac ctttggatta atttgcttgc ccgtcggttc gtctttatgg
     3121 ggggctcctg cttgtgagcg taaataactg ttagacgcac tgtcgtagct aaattgtgcg
     3181 ttatatttag gaccagataa tataagatta accgtattgg ctgttggggt ttgtggtaca
     3241 tcaattttgc gagcaaaacc tgtaaattta ctagatgtat atccttttga ttgattaagg
     3301 ctgtctaatc gtgcaaaatc actatacaca ttgtgtggag cagatttgct agatagtcta
     3361 gtgtaggcat tggcgttata aaactgatct aaatctttaa tgccaaggct ttttatatcc
     3421 tgaagcgctt ctgggctacc accaacgtga ccgtatgaac catctagtcc agcaatgtag
     3481 tcaacatagt acggacgcgc acttctaatc ggtccgattt tgtctggctg agattcttga
     3541 tacactgcca tgaacctggt ta
//
LOCUS       DAOPLS010000065         3489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_30084_length_3489_cov_3.192196, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000065 DAOPLS010000000
VERSION     DAOPLS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30084_length_3489_cov_3.192196"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..509)
                     /locus_tag="PKV52_02135"
     CDS             complement(12..509)
                     /locus_tag="PKV52_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99044.1"
                     /translation="MNNKPIFLHHYGDIRETIFQTIETLFRQYNHVHVAPNSGYSFVL
                     AHTHHDTTALELCSIGLVRLPTGVLIETAFPNQPKVHNRVWMIDANFGQITYIEITNP
                     DLRHSKELLFGDPDNNALELHAMPSDLRLLQTILTTALSAIKSTKYIGVSQPMNMQYM
                     PLLTQ"
     gene            complement(506..886)
                     /locus_tag="PKV52_02140"
     CDS             complement(506..886)
                     /locus_tag="PKV52_02140"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPD99045.1"
                     /translation="MIAVSDQEFEAYVTQSIDRLPSPYKENIKNIAFFVTDEPTMQQR
                     KRLGLRPCQSLYGLYEGVPLPARNGNDSNLLPDTITIFKIPHEQHANSEQELRLQIAN
                     TVWHEVAHYFGLDHARIHELENKL"
     gene            complement(958..2424)
                     /gene="cls"
                     /locus_tag="PKV52_02145"
     CDS             complement(958..2424)
                     /gene="cls"
                     /locus_tag="PKV52_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPD99046.1"
                     /translation="MQTEIASLLTLIAVLIIDIGVRVFALGVIPNNRKPSEATAWLLA
                     VFFFPLLGILIFLVFGHSKLSKKRRAKQLSINNLIYKNTLNKVAIAEENSPPNWFMSI
                     ATLNSNLGAMPLVGGNDVGLIQNYKQSLKDMVTAIDAAKKYVHVEFYIMSVDNTTKDF
                     FDSLRRAQERGVVVRVMFDHIGSLQYPGYKDMLSFFDQHDINYQLMLPIKPLQGKVQR
                     PDLRNHRKILVVDGVVGFMGSQNIIDSSYNKKKNIKRGLHWLELMVRVVGPVVYELDA
                     IFAGDWYAETDELLKTHIVSIGNPVVDKGNIDCQVVPSGPGFEGENNLKLFTSLIYAA
                     QQKIIIASPYFVPDESMLTAITTAVQRGIHVELYVSEIGDQLMVYHAQRSYYEALLKA
                     GVHIYMYKSPTVLHAKHFTVDDAVSVLGSSNMDMRSFNLNMEISLLIYSKEFVQAMRR
                     VETSYKIKSRQLHLDAWLHRPLRDKFVDNVARLTSSLQ"
     gene            complement(2466..>3489)
                     /locus_tag="PKV52_02150"
     CDS             complement(2466..>3489)
                     /locus_tag="PKV52_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPD99047.1"
                     /translation="IPIGLPVPDLPSIELYDLQNLIIPAMAMMLIAYANTIATARSFA
                     SRKKETVSSDQELLGLGLSNAASGIYGGIPVSASGVRTAVNYTSRAKTQVSQLFGALF
                     IGVVLAVLSPMLQYLPITMLAVIIILAVLPLFNVKELRSIWHAWRSEAVVAVVTMLGV
                     ATLGIYQGLLLAVMLAILNTLRKSAFPNDAILGVADDGSVRDMHRPPKTHEVPGLKMY
                     RFDAPLYFGNATYFRHRVLQIVDESKEPIHWFLWDAETITAIDSTSGQMLLGLIDELK
                     SRKVTFAVARMKGSIRTIVHRSHRLSSTLQHSPHYTSIGNAIEAYRQEYNVPRAYDVK
                     SEDKST"
BASE COUNT          997 a          722 c          708 g         1062 t
ORIGIN      
        1 agtacttgca attattgggt gagtagtggc atatactgca tattcatagg ttgtgatacc
       61 cctatgtatt ttgtactttt tatggcactg agggcagttg taagaatagt ttgtaacaaa
      121 cgtaaatcgc ttggcatagc atggagttct aaggcattat tatctggatc accaaacaat
      181 agctctttgc tgtgtcgtaa gtctggattg gtaatttcta tataggttat ctgaccaaaa
      241 ttagcgtcta tcatccacac gcgattatgc acttttggtt ggtttggaaa agcagtttca
      301 attaacacgc cagtcggtag ccttactaag ccaatagaac atagttctaa ggctgttgta
      361 tcgtggtgtg tatgtgctaa tacaaaactg tacccagaat ttggtgcaac gtgtacatgg
      421 ttatactgcc taaatagcgt ttcaatcgtt tgaaatatag tttcgcgtat atcgccataa
      481 tggtgcaaaa agattggctt attgttcata gtttattttc tagttcatgt atccgcgcgt
      541 gatctagccc aaaataatgt gctacctcgt gccacactgt gttggctatt tgcaggcgta
      601 attcttgctc gctgttggcg tgctgttcgt gtgggatttt aaaaatagta attgtatctg
      661 gcagtaaatt actatcatta ccattcctgg ctggtaatgg aacgccttcg taaaggccgt
      721 ataaagattg gcacggacgc aaccctaagc gcttgcgttg ctgcattgtt ggttcgtctg
      781 tcacaaaaaa agcaatattt ttaatgtttt ctttgtatgg ggagggaagc ctgtctatag
      841 actgagtgac gtatgcttca aattcttggt ctgatactgc tatcatagta ctctggtact
      901 atacgtagtt ctaagcatat tctcaagcta tagagcgtgt gtaagtatac gtaatactta
      961 ctgtaacgat gacgtaagac gggcaacatt atctacaaac ttatcccgga gtggccgatg
     1021 cagccatgca tcaaggtgca gttgacgact tttaatctta tatgatgttt ctacgcgtcg
     1081 catggcttgc acaaattctt tactataaat aagcagcgat atttccatat ttaaattaaa
     1141 tgaacgcata tccatattac tagagccaag cacacttacc gcatcatcta ccgtaaaatg
     1201 cttggcgtgc aatacggtag gcgatttata catatatatg tgtacaccag cttttaataa
     1261 cgcttcgtag taagaacgtt gcgcatgata caccattagt tgatcgccaa tttcagatac
     1321 atacagttct acatgtatgc cacgctgcac agctgttgta atagcagtga gcattgattc
     1381 atctggtaca aagtacgggc tggcaataat aattttttgt tgggctgcat aaataagtga
     1441 tgtaaatagt tttaagttat tttcgccttc aaaaccaggg ccacttggca ctacttggca
     1501 gtctatatta cctttatcta caaccgggtt acctatagaa acaatgtgcg ttttaagcag
     1561 ttcatctgtt tcggcatacc aatctcctgc aaaaatagcg tcaagttcat acaccacagg
     1621 gccaaccaca cgtaccatca gttctaacca atgaagccca cgtttaatat tcttcttttt
     1681 gttgtaactg ctatcaataa tattctgcga gcccataaag cccaccacac catcaactac
     1741 aagaatttta cggtgattgc gtaagtctgg ccgctgcacc ttaccttgca gtggtttaat
     1801 gggcaacatc agctggtaat taatgtcatg ttggtcaaaa aacgacagca tgtctttgta
     1861 gcctgggtac tgcaatgagc ctatgtggtc aaacataacc cgtactacta cgccgcgttc
     1921 ttgagcacgc cgtaaactat caaaaaaatc tttcgtagtg ttatctaccg acataatata
     1981 aaactctaca tgtacatatt ttttcgcagc atctattgct gtgaccatgt ctttaagaga
     2041 ctgtttgtaa ttttgtatta aaccaacgtc attgccaccg acaagtggca tagctcctag
     2101 gtttgaatta agcgtcgcta tagacataaa ccagtttggt ggtgagtttt cttctgcaat
     2161 tgctacttta tttagtgtat ttttataaat gaggttatta atagaaagct gcttggcgcg
     2221 tcttttttta gacaatttac tatggccaaa aacaagaaat ataagtatgc caagaagcgg
     2281 gaagaaaaag accgccagta accatgccgt tgcttctgat ggctttctgt tattaggaat
     2341 cacaccaagg gcaaaaactc tgacgccaat atcaataatt aagacggcaa taagcgttaa
     2401 caatgaggct atttctgttt gcatttagct acagtatagc aactctttag actcatacgg
     2461 taatatcagg ttgatttatc ttctgatttt acatcgtacg cacgtggcac attgtattct
     2521 tgccggtatg cctctatagc gttgccaata gatgtatagt gtggtgaatg ctgtagcgtg
     2581 ctacttaggc ggtgcgaacg atgcacaatt gttcttatag accctttcat cctcgcaacc
     2641 gcaaaggtta ctttacgtga ttttagctcg tctatgagcc ctagaagcat ttgcccagac
     2701 gtagaatcta tagcagttat tgtttctgca tcccataaaa accagtgaat aggctcttta
     2761 gattcatcta caatttgcag tacccggtgc cgaaaatacg tggcattgcc aaaatataga
     2821 ggtgcatcaa aacgatacat tttaagccct ggcacttcat gtgtcttggg tgggcggtgc
     2881 atatcgcgga ccgatccgtc atctgcaaca ccaagaattg catcgttagg gaaggcactt
     2941 ttgcgcaatg tattaagtat tgcaagcatc actgccaata gtaagccttg gtaaatacct
     3001 aacgtggcaa ctccaagcat tgttactaca gcgactactg cttcactacg ccatgcatgc
     3061 caaatagaac gtaactcttt tacgttaaac aaaggcaata ccgccaaaat tataatgacc
     3121 gccagcattg ttataggcaa atactgtaac attggtgata acaccgcaag taccacacca
     3181 ataaatagcg caccaaacag ttgcgaaact tgcgttttag cacggcttgt atagtttact
     3241 gctgtgcgta caccacttgc agacactggt ataccaccgt aaatgccaga cgctgcatta
     3301 gaaagcccaa gcccgagtag ttcttggtca gaactcactg tttctttttt cctagatgca
     3361 aacgaccgcg cggttgcaat agtattggcg taggcaatca acatcatcgc cattgctggt
     3421 ataattaggt tttgcaaatc atataactca atagacggca ggtcgggtac aggtaagcca
     3481 ataggaata
//
LOCUS       DAOPLS010000066         3480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_30201_length_3480_cov_5.417226, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000066 DAOPLS010000000
VERSION     DAOPLS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30201_length_3480_cov_5.417226"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..701
                     /locus_tag="PKV52_02155"
     CDS             138..701
                     /locus_tag="PKV52_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99048.1"
                     /translation="MSEKLRHNHEQQSNHTEKKPYHHETIRKHETHTSPERTHSKEAI
                     YKTIESQAKSKDSLNITHTEKEHHQRPHHYITRSVKLGVYTHTIKRVQSQLKPTERVF
                     SKIIHNDTIETISELGADTVGRSSAILGGGIVMVFAGLGLLLTARYFGFTIPLSSLIV
                     LYVIGFVLVFIVDIASKPVKNRRRKHN"
     gene            complement(726..>3480)
                     /locus_tag="PKV52_02160"
     CDS             complement(726..>3480)
                     /locus_tag="PKV52_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD99049.1"
                     /translation="DLESKGRDARDIADESIFKAQTLYNILASATKKGFKSNLYGQRH
                     ISLEIVAHKGKVYFYTAAPITLVPVIEQAILSAYPNSRLEEVAEHNIFSPVGKASGTI
                     GGELVLKESSVYPIATYQEIKRDTMQALLNALSSLEREDGAGVQILIRPTSSGWTKLA
                     STESSNLRQGKKNRKGADLAFWWVRQIFTAPVKPPEAKEGQNSVEPPTVSGTDQARAE
                     AIEEKARHNGFEVLVRVVASSNISHRSQVILNNIVAVFSLYEATGRNGFKFIPAKDID
                     SFVLAYILRFFPPEYRKSILNDVELATIFHLPDSSNVPTTQLERQQSKQVDGPRNLLE
                     DGLLLGYNVFRGAKKPIRLSENDRRRHMYIVGQTGVGKSVFLENLALQDMIEGRGFAF
                     IDPHGDTAETLLGMVPKERTEDVIYFCPSDMDYPLGLNLFEYSLPEQKDFLIQEVISM
                     LYKLYDPQHQGIIGPRYEHIFRNCALLLMADPHGGTFIDIPKLLTDYDYAKQKLKYTN
                     DRMVLDFWTKEFPNSQRSNESGEVTSWVVSKFGAFMSNEMMRNIIGQNTSSFDLRKIM
                     DEGKILLVNLSKGRTGELNSKLLGMIFVMKFQAAAMSRADIPEAQRRDFSLYVDEFQN
                     FSTDSFATILSEARKFGLNLIVANQFTTQLTEEVRDAIFGNIGTVISHRVGNQDAEVL
                     ERYFRPIFDIDDLQQLPNYNTIVKMLIGGVPTQPFSMANLPPLGHTNKQLSDALKQLS
                     AVKYGRPKAIVEKEIFDRLTTEEKPKPTLGARGGAFGGGIAPPKPPGQSSFLDDWLAK
                     RRAQDAKRITNPAMAAGPQQTPAQAAPTTPKAAETVPKGDISTNRNISSKTVDNQEID
                     SIANKLKESLNTKTDTTSSSQQGTSTQKDSIVAKTGTKGMFSHDDTIHIDKNGNLTSK
                     TSS"
BASE COUNT          949 a          757 c          694 g         1080 t
ORIGIN      
        1 aatactggtc caggaagcac gttactaatg agtacactga tagtagtcgt tgttgcctat
       61 ttttactatc gtaataaact actctcaaaa gaattgggta ttattcaaaa agagtttcaa
      121 gcgggaggac tataggtatg tcagaaaaac ttcgccacaa ccacgaacaa cagtctaatc
      181 acactgagaa aaaaccatac caccacgaaa ctattcgtaa acacgagacg cacacgtctc
      241 ctgaacgtac acattctaaa gaggctattt ataagacaat agaaagtcag gctaaaagca
      301 aagatagctt aaacataacc cacacagaaa aagagcatca tcagcgacct catcactata
      361 ttacccgcag cgttaaactt ggtgtgtata cccacacaat taagcgtgta caaagccagc
      421 taaagcccac agagcgcgtt tttagtaaaa ttatacataa cgatactatt gaaactataa
      481 gcgagcttgg cgcggacact gtgggacgtt ccagcgcaat tctaggcgga ggcatcgtca
      541 tggtcttcgc tggactcggc ttactactca ctgcccggta ttttggtttt acaataccct
      601 taagtagcct cattgtgtta tatgtaatcg gttttgtatt agtatttatc gttgatatcg
      661 caagcaaacc tgtaaaaaat cggcgccgta aacacaacta acgcacgtat ttcttttttc
      721 gttttttatg aactggtttt agaggttaga ttaccattct tgtctatatg tattgtgtca
      781 tcatgagaaa acataccttt tgtaccagtt tttgccacaa tactatcttt ttgggtagag
      841 gtaccttgtt gagaactact tgtggtatct gtttttgtat tgagtgattc tttgagttta
      901 tttgcaatgg aatctatttc ttggttatct acagttttac tactgatatt tcggttggta
      961 gaaatatcgc cttttggtac tgtctctgca gccttggggg ttgttggggc tgcttgagct
     1021 ggtgtttgtt gtggcccagc cgccatcgcc ggattcgtaa tacgtttggc gtcttgcgct
     1081 cggcgctttg ccagccaatc atctaaaaac gagctctggc cgggcggctt aggaggtgca
     1141 atccctccac cgaaagctcc tccccgcgca cccagggttg gtttgggttt ttcttctgtc
     1201 gtgagtctat cgaagatttc tttctccaca atcgcttttg gtctaccgta tttaactgct
     1261 gaaagctgtt taagagcatc tgatagttgc ttgtttgtat ggcctagtgg cgggaggtta
     1321 gccatactga aaggttgcgt gggcacgcct ccaatgagca tttttacaat tgtattatag
     1381 ttgggtagct gctgtaagtc gtctatatca aagataggtc taaagtaccg ttcaagcact
     1441 tctgcatctt gattaccaac acgatgtgaa ataacggtac caatgtttcc aaaaatagca
     1501 tcccgaactt cttcagttag ttgggtggta aactggttcg ccactataag gtttagtcca
     1561 aatttacgag cctcacttaa aatggtagca aaagaatctg ttgagaaatt ttgaaattcg
     1621 tctacatata aactaaaatc cctccgctga gcttctggta tatctgcccg gctcatggct
     1681 gctgcttgaa atttcataac aaaaatcatc cctaaaagct ttgagttaag ctcacctgtc
     1741 cgccccttac ttagattaac cagtaatatt ttaccttcgt ccatgatttt tctaaggtca
     1801 aaggaactag tattctggcc aataatgtta cgcatcattt cgttagacat aaaggctcca
     1861 aacttgctaa cgacccagct tgtcacttct ccggattcgt tagaacgttg tgaatttgga
     1921 aactctttcg tccagaaatc tagcaccata cgatcatttg tgtatttcag tttttgtttt
     1981 gcgtaatcat agtccgtaag tagtttaggt atgtctataa aagttccacc gtgaggatct
     2041 gccataagga gtaatgcaca atttctaaag atatgttcgt agcgtggccc aataattcct
     2101 tgatgttgag ggtcatataa tttatacaac atagaaatta cttcttgaat caaaaagtct
     2161 ttttgttcag gcaagctata ttcaaataag ttaagcccaa gaggataatc catatctgac
     2221 ggacaaaaat aaattacatc ttctgttctt tcttttggca ccattcctag cagagtctca
     2281 gctgtgtcac cgtgaggatc aataaaagca aacccacgac cttctatcat atcttgcagc
     2341 gcaaggttct ctaaaaatac agatttaccg acaccggtct gaccgacaat atacatatgg
     2401 cggcggcgat cgttttcgct cagcctgata ggctttttgg cacccctaaa aacgttgtag
     2461 ccaagtaaga gcccatcttc aagcaaatta cgaggtccat caacttgttt tgactgttga
     2521 cgctcaagct gtgtggttgg gacattagaa gaatctggca aatgaaatat cgttgctagt
     2581 tctacatcat ttaaaatact ctttctgtat tctggtggga aaaatcgtaa aatatacgcg
     2641 agaacaaagc tgtctatatc cttggctgga ataaatttaa acccatttct gccggtagct
     2701 tcatataaag aaaatactgc gacaatgttg tttaaaataa cttgcgatcg gtgcgaaata
     2761 ttagacgacg ctactacccg aaccaagact tcaaaaccat tatgccgagc tttttcttct
     2821 attgcttctg cgcgggcttg atcggtacca gaaacagttg gtggttctac tgagttttga
     2881 ccttcttttg cctctggtgg tttaaccggc gctgtgaata tttggcggac ccaccaaaaa
     2941 gccaaatctg caccttttct atttttcttg ccttgccgta aattacttga ctcggtactg
     3001 gctagtttgg tccagcccga gctggtaggc ctaataagta tttgaacccc cgcaccatct
     3061 tctctttcta gtgacgacaa ggcgtttaac aaggcctgca tagtatcacg cttaatctct
     3121 tgatacgtcg ctataggata taccgacgat tcttttagca ctaattcccc accaatggta
     3181 ccgctcgctt taccgacagg gctaaatata ttatgttcgg ctacttcttc taaccggcta
     3241 tttgggtagg cactcaagat tgcttgttct attaccggca ccaatgtaat tggagcagcc
     3301 gtataaaaat acacttttcc cttatgtgct acaatttcta agctaatatg tcgctgacca
     3361 tacaagttac ttttaaagcc tttcttggtg gcactcgcca gtatgttata cagagtttgc
     3421 gccttgaata tactctcgtc ggctatatcg cgagcatcgc ggcctttgct ttctagatcg
//
LOCUS       DAOPLS010000067         3431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_30798_length_3431_cov_3.860190, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000067 DAOPLS010000000
VERSION     DAOPLS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30798_length_3431_cov_3.860190"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1942
                     /locus_tag="PKV52_02165"
     CDS             53..1942
                     /locus_tag="PKV52_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPD99050.1"
                     /translation="MTAQHIRKAYLEYAKQNGHTIIPRASLVPQNDPTTLFTGSGMQP
                     LLPYLLGEQHPSGTRVVDSQPCFRSEDIEEVGDNRHTTFFEMLGNWSFGDYFKQDQLT
                     WYFAFLVDIVGLDPNKLYVTAFIGDSAAGVPKDSESAELWVELFKSKDITANIADIGS
                     EEDGYKRGIKQNERIFYYDAKKNWWSRAGVPANMPAGEPGGPDSEVFYEFSHIQHDTS
                     YGEHCHPNCDCGRFMEIGNSVFMQYIKNDDGSFSELPKANVDFGGGLERVAAASEGSG
                     DMFKISLLWPIIQKLEQLSGKKYEQHSTAMRVIADHLRAATFLAVDGVVPSNKTQGYV
                     IRRFLRRAIRYAFELGIEQNFLEQVVPTIADLYHDDYPEVAQNREQIIAVLAREEKVF
                     RQTLRSGVREFGKLTSGGLLTGAIIFKLYDTYGFPVELTKEEAFKQDVAVALDATEEF
                     DRFMAEQKARSQTAAKGVFKGGLADHSEITTKYHTATHLMYKALRIVLGDHVVQRGSN
                     ITAERLRFDFSHDQKVTPEQITQIEAIVNEQIKKDWPMSWREESTKEALANGVMGAFG
                     DKYGKTVKVYTVGDPDGDNYSREICGGPHVTHTGKLADDGKVFKITKEQASSAGVRRI
                     KAILM"
     gene            complement(1948..2829)
                     /locus_tag="PKV52_02170"
     CDS             complement(1948..2829)
                     /locus_tag="PKV52_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99051.1"
                     /translation="METERNYFRVNLLPEDVTSLNSLIKMVCTAKKHPYKYRHIKIDV
                     PGNFGMVDTSQITMYGSGYSSRDITLLDAGKDDDSADSGLMSCMLESHRKSGHDAIID
                     YDWKNKEAKLIPESEPWHYGMGECLVERYFDRRYHDAVKYMNDSTANQIEDLVGEQDE
                     KLSPFDIITRLIQHDNAAVTVEQFFSADYTGERLSHHEPKTHNQAQLRLIEYLKGTKA
                     LSTNFKLGIFTLLAVADNEDAVDLAYVLSANYDSQHDEVHDLTFEASIQAPLAITRIP
                     RISPFWEFGDAIAKIGY"
     gene            2937..>3431
                     /locus_tag="PKV52_02175"
     CDS             2937..>3431
                     /locus_tag="PKV52_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99052.1"
                     /translation="MQEESGQSTTSNSQEEPKPETKTKKSHTGRNVFIGLAIGLIIVI
                     GVPVLIAGYFGLVPGVSTLMGATKAKDLGVTYTPQDYQAFLTKTQTNLQNYANAPVSP
                     DNPAKKTVFADPVVVNNQVVSQEELTAAINESGWLWMPIKNAQVKLTDGTVEIAGNLQ
                     LDYIT"
BASE COUNT         1088 a          697 c          718 g          928 t
ORIGIN      
        1 ttttaacaac ggcttgtata tgccttagaa cctctgtaaa ataataggta atatgacagc
       61 acaacacatt agaaaagcct acttagagta tgccaagcaa aatggacaca ccataattcc
      121 acgggcaagt ttagtaccac aaaatgaccc aaccactctg tttacaggga gtgggatgca
      181 gccattacta ccatacttac ttggcgagca acatcctagt ggtaccagag tcgtcgatag
      241 ccaaccatgt tttagatcag aagacataga agaagtcggc gataaccgcc acactacatt
      301 ttttgaaatg ctcggcaact ggagctttgg tgattacttt aagcaagacc aactcacctg
      361 gtattttgca tttttagtag atattgttgg cctagaccca aataaactgt acgttacagc
      421 gtttataggc gacagcgcag caggtgttcc aaaagattca gaatcggccg agctgtgggt
      481 agaactattt aaaagtaaag atattacggc aaatattgca gatattggtt ctgaagaaga
      541 cggttacaag cgcggcataa aacaaaatga aagaatcttt tactatgatg caaaaaagaa
      601 ctggtggagc cgggctggtg tacctgcaaa catgcctgct ggtgagccgg ggggcccaga
      661 cagcgaagta ttttatgaat ttagccacat acaacatgac acgtcgtatg gtgagcactg
      721 ccatcctaat tgcgattgtg gtaggtttat ggaaattggt aactctgtat ttatgcagta
      781 catcaaaaat gacgatggca gttttagcga actaccaaaa gctaacgtag attttggtgg
      841 tggtttagag cgagtggctg ctgcttctga aggcagcgga gatatgttta aaataagttt
      901 gctgtggcca attatacaaa agctagaaca gctaagtggc aaaaagtacg aacaacacag
      961 taccgccatg cgcgtgattg ctgatcactt acgtgcggca acctttttag cagtagacgg
     1021 ggtagtgcca agcaataaaa ctcaaggcta cgtgatacgc cgttttttgc gtagagccat
     1081 ccgctacgcc ttcgaacttg gcatagagca aaacttttta gagcaagttg taccaaccat
     1141 tgccgattta taccacgacg attacccaga agtcgcccaa aatcgcgaac aaataattgc
     1201 agtgttagca cgtgaagaaa aggtattccg ccaaacgctc cgcagtgggg taagagaatt
     1261 cggcaaacta acgagcggtg gcttattaac aggtgcgatt atattcaagc tatacgacac
     1321 atatggcttt ccggtagaac tcaccaaaga agaagctttt aagcaagatg tcgcggttgc
     1381 gcttgatgcc acagaagaat ttgatagatt tatggcagaa caaaaagctc gtagccaaac
     1441 tgctgcgaaa ggtgtcttta aaggtggttt ggctgaccac tcagaaatta caacaaaata
     1501 ccacacagcc acacacctca tgtataaggc tttgcgtatt gtgttgggtg accacgtagt
     1561 gcaacgtggt agcaatatca ctgccgaacg tttacgtttt gactttagcc acgatcaaaa
     1621 agtaactcca gaacaaatta cacaaataga agcaatcgtg aacgaacaaa tcaaaaaaga
     1681 ttggccaatg tcatggcgcg aagaatcaac caaagaagca cttgcgaatg gtgtgatggg
     1741 tgcatttggt gataaatacg gcaaaacggt aaaggtctat actgttggtg acccagatgg
     1801 cgataattac tcacgcgaaa tttgcggtgg cccacacgtg acgcatactg gcaaattagc
     1861 agatgatggt aaagtgttta aaatcaccaa agaacaagca agttctgccg gtgttcgccg
     1921 tataaaagcc atacttatgt aatgaattta atagccaatt tttgcaattg catcaccaaa
     1981 ttcccagaaa gggctaattc ttgggattct ggttattgct agaggcgctt gaatactagc
     2041 ctcgaaagtc aagtcatgca cttcatcgtg ctggctatca taattagcag ataaaacata
     2101 cgcaagatca actgcgtctt cattgtcagc cacagctaat aaagtaaata tacccaattt
     2161 aaagtttgtg cttagggctt ttgtgccttt aagatattca attaaacgaa gttgagcctg
     2221 attatgcgtt tttggttcgt gatggctcaa acgctcacca gtataatctg cgctaaaaaa
     2281 ctgttctaca gtaacagctg cgttatcgtg ctggattagt cttgtaatta tatcaaaagg
     2341 agataatttc tcgtcttgtt caccaacaag atcttcaatc tgattcgccg tagagtcatt
     2401 catatattta acagcatcat gatagcgcct atcaaaatat ctctcgacaa gacattcacc
     2461 catgccgtaa tgccaaggct cggactcagg aattaatttt gcctctttat ttttccagtc
     2521 gtaatcgata atagcgtcat gaccgctttt tctatgagat tctagcatac agctcatcaa
     2581 gccactgtct gccgagtcat catctttccc tgcatctaat aatgtaatat ctctactact
     2641 ataaccgcta ccgtacattg taatctgact tgtatcaacc attccaaaat tacccgggac
     2701 atcaattttt atatgcctgt atttgtaagg atgttttttt gctgtgcaga ccatttttat
     2761 gagactattt aaagaagtga catcttcggg cagtaaattt actcgaaaat aatttctttc
     2821 agtttccata atatatacta tatcatatta taaaacttat gtctagtttt acgttcattt
     2881 tagctgtatt tggtctatac aacgggcgta taataaaaag taaaggagaa cgtgttatgc
     2941 aagaagaatc tggtcaatct acaacaagta atagccaaga agaaccaaaa ccagaaacaa
     3001 agactaaaaa gagccacaca ggcaggaatg tttttattgg tttagcaatt gggctcatta
     3061 ttgtcattgg tgtgccggta cttattgctg gttattttgg gcttgtgccg ggtgtatcta
     3121 cgcttatggg tgccacaaaa gctaaagatc taggtgttac ctatacacca caagactacc
     3181 aagcgttttt aaccaaaact caaactaatt tgcaaaatta tgctaacgcg ccagtaagcc
     3241 cagataatcc agccaaaaag accgtttttg cagaccctgt ggtcgtaaat aaccaagtgg
     3301 tcagccaaga agaactaaca gcagcaatta atgaatcagg ctggctatgg atgcctatta
     3361 aaaatgccca ggttaagctt acagatggca ccgtagaaat tgccggtaat ctgcagctag
     3421 actacattac c
//
LOCUS       DAOPLS010000068         3407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_31104_length_3407_cov_3.374105, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000068 DAOPLS010000000
VERSION     DAOPLS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31104_length_3407_cov_3.374105"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1753)
                     /gene="groL"
                     /locus_tag="PKV52_02180"
     CDS             complement(113..1753)
                     /gene="groL"
                     /locus_tag="PKV52_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379543.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPD99053.1"
                     /translation="MAKRVFYDEDARRRVLAGAEVLYNAVKTTMGPKGRNVVISKSYG
                     APSVTHDGVTVAKGVDLPEEDDETLGYKVGAELIKQAANKMNDMAGDGTTTVTVLTYH
                     ILSEANKLIAAGHNPMQLRKGLESAAHEVIKELSGVAEDIKDDKKRVAEVATISAGDS
                     EIGALIADVIAKVGKDGVVTVEQGQGLGLESEVVEGFTIDRGFVSPYFVTDPNRMEAV
                     YEKPAIVVTDKKITSVQEFLPMLEKLAQAGKKDLVLVAEDVEAEALATLVLNKLKGVF
                     NTVAVKAPAFGDRRKDILQDTAILTGATVITEEQGHTFENADLSFVGSARKIIVTKDD
                     TTIIEGSGNPVEVDARIKQIGAQADAATSEYEKENLDKRRAALKGKVAVIKVGGATET
                     EIEEKKYRVDDAVAAVKAALDEGIVSGGGVTLVNAAAVVKVTGTDAESAGKQLLKNAL
                     EQPFRILLTNSGLNADEWLPQVRLAKAGQGIDVNKPNKLVDLKAAGIIDPARVTKEAV
                     QNAVSIAGTTMTMGALVVEIPEKAAPAAPDMGAMGGMM"
     gene            complement(1834..2100)
                     /locus_tag="PKV52_02185"
     CDS             complement(1834..2100)
                     /locus_tag="PKV52_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013138933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPD99054.1"
                     /translation="MSVPIHPLADYVVAQQEEAESKTASGLYLPEKAAEKPKTAKVLA
                     VGKKVDGIKAGDRIIYKSYSSTDVKVDGVEYLIIDEEDILATVK"
     gene            complement(2201..2884)
                     /locus_tag="PKV52_02190"
     CDS             complement(2201..2884)
                     /locus_tag="PKV52_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99055.1"
                     /translation="MTSGANKAITQFNSEQERQLSAEAFEARNRLIETVYTFLDIALQ
                     ENKDQYANMQAAFARGKATYTFDHYVVTSDQDEHLAQRGDDYRPTILLRFKNVHNTAP
                     SHQVNSTTEIIIAIPTQDDFTVDRTTDISDIDLSDKSIYVYWNVGDDSESCHEISAEG
                     VRKAQLDINNAHTPETTFDFIYAPHFAPLDTAAALVARLRQDLRNLDLVPHFRVTVDA
                     SGSEISRQT"
     gene            2954..3253
                     /locus_tag="PKV52_02195"
     CDS             2954..3253
                     /locus_tag="PKV52_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99056.1"
                     /translation="MSNPVRKCTYNDTKKKVDTFEGTTRSKRHTGRAVVPHQWATAHR
                     RRAHEAAQPIAKNWWVAWQPYSEEWSDPSTAGKHGDRKSRSAPGQGGARHRTERS"
BASE COUNT          856 a          781 c          720 g         1050 t
ORIGIN      
        1 agttatagtt gcaatttcaa ataattgttg cataactaaa tgcttatgta aaagacccct
       61 tgtgttgtac gaggggtctt tcttgtgtag caactcttaa gccgagcggg tgctacatca
      121 taccacccat ggctcccata tctggagcag caggtgcagc tttttctggt atttctacta
      181 ctagtgcgcc cattgtcatg gtggtaccag ctatagatac cgcattctgg actgcttctt
      241 tagtaacgcg tgctgggtca ataataccag cagcttttag atcaacaagt ttatttggtt
      301 tgttgacatc aatgccctgc ccagccttgg ctaagcgtac ttgtggtaac cactcatcgg
      361 cgtttaagcc agagttcgta agtaaaatac ggaatggttg ttcaagcgcg ttctttagta
      421 gctgcttgcc agcagattca gcatcggtac cagtaacctt aacaacagct gcagcattta
      481 ctaatgtaac gcctccacca gatacaatac cttcatctag cgctgcttta acggcagcaa
      541 cggcgtcatc tacgcggtat ttcttttctt caatctcggt ttctgtggca ccaccaactt
      601 taataacagc tacttttcct ttaagggcag cacggcgctt atcgaggttt tctttttcgt
      661 attcactagt agcagcgtct gcttgagcac caatttgctt aatacgggca tcaacctcta
      721 ctgggttacc agaaccttct atgattgttg tgtcgtcttt ggtaacaatg attttacgag
      781 cactaccaac aaaacttagg tctgcgtttt caaaggtgtg accttgttct tctgtaataa
      841 ccgttgcacc agtaagaata gcagtatctt gtaatatatc tttacggcgg tcaccaaaag
      901 ctggcgcctt aacagcaaca gtgttaaata caccttttag tttattcagt accaatgttg
      961 ccaacgcttc tgcttctaca tcctctgcaa caagcactaa atctttttta cctgcttggg
     1021 caagcttttc tagcatgggg agaaattctt gtacgctagt gatcttttta tcagttacaa
     1081 caatagctgg tttttcgtat acagcttcca tgcggtttgg gtcagtcaca aaatacgggc
     1141 tcacaaaacc acggtctatg gtaaagcctt ctacaacttc actctcaagg ccaagcccct
     1201 gcccttgttc tacagttacg acaccgtctt taccaacctt ggcaatcacg tctgcaataa
     1261 gcgcgccaat ttcactatcg cccgcgctaa tggtagctac ttctgcaaca cgttttttat
     1321 cgtctttaat gtcttctgct acgccagaaa gttctttaat aacttcgtga gcagccgatt
     1381 ctaaaccttt acgtagttgc attgggttat ggccagcagc aatgagcttg ttggcttcgc
     1441 ttaagatgtg gtaggtaaga acggttacag tagtagtacc atcacctgcc atgtcattca
     1501 ttttgttcgc agcttgttta ataagttctg caccaacctt gtagcctaat gtctcgtcgt
     1561 cttcttctgg caagtctaca cctttggcaa cagtaacacc gtcgtgcgtg acactgggag
     1621 cgccatagct cttgctaata accacattcc ggcctttagg gcccatggtg gtctttactg
     1681 cgttatataa tacttctgca ccagccagca ctctgcggcg agcatcttca tcataaaaaa
     1741 ctctttttgc catttataat ctccttggtt tatttactat tgaatcgtca gtaaatgagt
     1801 ttacgtaaac tactcactac ctttttatct tgcttactta acggtggcta aaatgtcttc
     1861 ttcgtcaata attaaatact caacgccgtc tacttttaca tctgtagaac tgtaactttt
     1921 gtatataata cggtcgccgg cttttatgcc atctactttt ttaccaacag caagtacttt
     1981 tgcagtttta ggcttttctg ctgctttttc tggcaggtac aaaccgctgg cggtcttact
     2041 ttctgcttct tcttgctgag caacgacata gtcagcaagt gggtgtattg gtacgctcat
     2101 tatttcctcc atatttatca gttatacata tatattagca ctctaatgtc ctaagtgcta
     2161 atatatcgta cctcagtttg tttttaagcg tcaagacttt ttaagtttgg cgactaatct
     2221 ctgagccaga cgcatctaca gtaacgcgaa agtgcggtac aagatctagg ttgcgcaagt
     2281 cttgccttaa acgcgctaca agcgctgcag cggtgtctag tggggcaaaa tgtggagcat
     2341 aaataaagtc aaacgttgtt tctggagtat gtgcgttatt gatatcgagc tgggccttgc
     2401 gtacaccttc tgctgatatt tcatgacaac tttctgaatc atcgcctaca ttccaataca
     2461 cataaattga cttgtcggaa aggtcaatat cactaatatc tgtggtgcgg tctacggtaa
     2521 aatcatcttg agttggtatg gcaataatta tttctgttgt actattcacc tggtgactcg
     2581 gcgctgtatt atgtacgttt ttaaaccgta gcaatatggt tgggcggtaa tcatcgccac
     2641 gttgtgctaa atgttcgtct tgatctgacg ttactacgta gtggtcaaac gtataggtag
     2701 ctttaccgcg cgcgaaagca gcttgcatgt tggcgtactg atctttattt tcttgcaagg
     2761 caatatctag gaaagtatag acagtttcaa tcaggcgatt ccttgcctca aacgcttcgg
     2821 cagagagttg tctttcttgt tcgctattaa actgagttat cgccttattt gctccactag
     2881 tcataaacat attagaacaa aatatatttt atttgtcaat ttatttaaaa tagtgtataa
     2941 cagtacacag cttgtgagca atcccgttcg caagtgtacc tacaatgata caaagaagaa
     3001 ggtagatacc tttgaaggta ctaccagaag taaacgtcat acagggagag ctgtcgttcc
     3061 acatcagtgg gctacagctc accggcgaag agctcacgag gctgctcagc cgattgcaaa
     3121 aaattggtgg gtggcgtggc aaccctactc tgaagaatgg agtgatccga gtacggccgg
     3181 caagcacggt gatcggaaaa gtcgatcggc tccgggacaa ggtggagcgc gtcatcgcac
     3241 ggagcgctcg tgaagcagag ctgtacaagc ttgatccacg tacgtacatc acgtgggtca
     3301 agggcaaccg ctacactgtt cggttgctga cggcgtttgg ccttagcgag gaagacgtcc
     3361 gtgagctagt cgcaatcttg cgctcgaacc gcaaggtgaa agacgtg
//
LOCUS       DAOPLS010000069         3404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_31149_length_3404_cov_4.039116, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000069 DAOPLS010000000
VERSION     DAOPLS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31149_length_3404_cov_4.039116"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="tsaE"
                     /locus_tag="PKV52_02200"
     CDS             <1..412
                     /gene="tsaE"
                     /locus_tag="PKV52_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPD99057.1"
                     /translation="LGRAIGRSLKGGEVIQLVSDIGGGKTTLTKSIVAGTGCKAVVAS
                     PTFTVSKEYQAAHLKIRHFDFYRLQDAGVVGIELQEGFSDPSTVTIVEWSDIVKDVLP
                     TDTITITIQHTKEHERLFTIDVPTNKQYILRELA"
     gene            409..798
                     /gene="tsaB"
                     /locus_tag="PKV52_02205"
     CDS             409..798
                     /gene="tsaB"
                     /locus_tag="PKV52_02205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPD99058.1"
                     /translation="MILAVRTDKLEAELYVYKHANCVASYTWEAHRQLADTIHTKIHT
                     ILVNNDADWSSLQGLIVYKGPGSFTGLRIGVTVVNTLGYSLNIPIAGTTGENWLTEGL
                     VALQKVKQYTPVLPDYGGHIHITQPKK"
     gene            967..2481
                     /gene="rny"
                     /locus_tag="PKV52_02210"
     CDS             967..2481
                     /gene="rny"
                     /locus_tag="PKV52_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPD99059.1"
                     /translation="MEPIGLLLAVIAAGAGFGASQVVTRRKIGTAANEAEKQLAKANK
                     EAAAITRKAEEEAARKAEDARKADQQLRKELKDKESRIIDRETTLDKKLDELDKRSEK
                     LRYNEDEVESLKNEIRELRVKQQEKLEKVAKLTKKEAADKLMQMTERDIKNDLAGLIA
                     KLQNDAKDLAEETAGMIIVSAMERMASDVTAERTVTAVKLEDEEMKGRIIGKEGRNIQ
                     ALQRATGVDILVDDTPGMIILSSFDPIRREVARVALEMLLKDGRVHPGRIEEVVEKAQ
                     KHVHKEVIRAGEDAAREVGVVGIPRELLQLLGELKYRTSFGQNVLKHSTEMAHMAGII
                     AEQIGADVRVCKYAALLHDMGKALTHKIEGKHHHISAEMARKYGVPDDIVHAIEAHHD
                     DIEATTVEALVIRVVDAISAARPGARNISAENFAERMRELENVATSFSGIDKAYAISA
                     GREIRIIVQPNTVDDLSAIKLARDIATKIELTMQYPGTIKVNVIRETRAIEFAK"
     gene            2502..3293
                     /locus_tag="PKV52_02215"
     CDS             2502..3293
                     /locus_tag="PKV52_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPD99060.1"
                     /translation="MNILYIGDVMGQPGRKALAKVLPGLRISREIDVVIAQAENVSHG
                     KGMSLKHYRELEESGVDGFSGGNHSFERPDTVKLVQDTNSTVVAPANVKGPLENNYKI
                     IKNGVKKVAIVSLLGYTFPRGYNEDTINPLMCIDALLPKIQKNMPDAIIVNIHSDLSS
                     EKVMMGHYLDGKVTATVGDHWHIPTADTRLLPKHTAHVTDVGMCGVLNSSLGVDVDVA
                     LRRWKGEQVKNVMAKEKELQFNAVLIGTAPGSLMADSIERIQYYL"
BASE COUNT         1111 a          621 c          820 g          852 t
ORIGIN      
        1 gctcggtaga gcaattggtc gctcattaaa aggtggtgag gttattcagc tcgtgagtga
       61 tattggtggt ggcaaaacga ctttaacaaa aagtattgtt gcgggtactg gttgcaaggc
      121 ggtggtagca agccctacgt ttactgtttc aaaagagtac caagcagctc atctaaagat
      181 tcgtcatttt gatttttacc ggttgcaaga cgctggtgtg gttggtatag agctacaaga
      241 gggttttagc gatccaagca cggtgactat tgtagaatgg tcagatattg ttaaggatgt
      301 actaccaaca gacaccataa ctatcacgat tcagcacacc aaagagcacg aacggctatt
      361 taccatagat gtacctacga acaaacagta tattttaagg gaactggcat gatattagct
      421 gttcgtacag acaaactaga ggcagaactt tatgtataca agcacgcgaa ttgtgttgca
      481 tcgtatacat gggaagcgca tcggcaactt gctgatacta ttcatactaa aatacatact
      541 attttggtaa ataatgatgc agactggtca tcgttacaag ggctcattgt ctataaaggt
      601 ccgggcagtt ttaccggact tagaataggc gttaccgttg taaatactct cggctattca
      661 cttaatattc ctattgccgg aactactggt gaaaattggc tcacggaagg cttggtagca
      721 ttgcaaaaag ttaaacaata taccccagta ttaccagact atggtggcca tatacacatc
      781 actcagccaa aaaaataagt ttgtttattt acttgtgtca ggagtatgat agtacgtagg
      841 atatacacac tacagtgaag gtgatgtcca gacttgatta cttgattaat ttgattatcc
      901 taaaaaagct aatgatgtga tagtgatgct tttgtagggg tggtctgccg aacagaaagg
      961 gagaatatgg aaccaatagg attattattg gccgtaattg cggctggtgc tggttttggt
     1021 gccagccagg tagtaacgcg aagaaaaata ggtacagcag ctaacgaagc agagaaacag
     1081 cttgccaaag ccaataaaga agctgcagca atcacgcgta aagcagaaga agaagctgct
     1141 cgtaaagcag aagacgcacg caaagcagat cagcaactac gcaaagagtt aaaagacaaa
     1201 gaatcgcgca taatagacag agaaactacg ctagataaaa agcttgatga gttagacaag
     1261 cggtctgaaa agttgcgcta taatgaggac gaagtagaat cattaaaaaa tgaaattaga
     1321 gaattgcgtg ttaagcaaca agaaaagcta gaaaaggttg ctaagctcac caaaaaagaa
     1381 gcagccgata agctcatgca aatgacagag cgagatataa aaaatgatct tgccggttta
     1441 attgctaaac ttcagaatga cgccaaagat ctagcagagg aaaccgctgg aatgattatt
     1501 gtaagcgcca tggagcgtat ggcaagcgac gtgacggcag aacgaacagt taccgccgta
     1561 aagctagaag acgaagaaat gaaaggtcgc atcataggta aagaaggccg taatatacaa
     1621 gccttacagc gtgccactgg cgttgatatt cttgtagatg atacacccgg tatgattatt
     1681 ttaagcagtt ttgaccctat ccgccgcgaa gtagcccgtg tagctttaga aatgcttctt
     1741 aaagatgggc gtgttcatcc tggccgtata gaagaagtgg tagaaaaagc tcaaaaacac
     1801 gttcataaag aagtcattcg ggctggtgaa gatgccgcaa gagaagtagg tgttgttggc
     1861 ataccacgtg aactattaca gctgcttggt gagttaaagt atcgaaccag ttttggccaa
     1921 aatgtattaa agcattctac agaaatggcc catatggctg gtattattgc cgaacaaatt
     1981 ggtgcagacg tgcgtgtctg taaatatgct gcgctcctac acgatatggg caaagcgctt
     2041 actcataaaa tagagggcaa acaccaccat atttctgcag aaatggctcg taaatacggt
     2101 gtgccagacg acatcgtaca tgcgatagaa gctcaccatg atgacataga agctactact
     2161 gtagaagcac ttgtcatacg cgttgtagac gcaataagtg cagcacgccc aggtgccaga
     2221 aatattagtg ccgaaaactt tgcagagcgt atgcgtgaac tagaaaatgt tgcaacaagc
     2281 tttagtggta tagataaagc atatgccatc agcgctggcc gcgaaataag aatcattgta
     2341 cagccaaata cagtcgatga tttaagcgct attaaactag cacgagatat cgctacaaag
     2401 attgagttaa ctatgcagta cccagggact attaaggtta acgttatccg cgaaactcgt
     2461 gctatagaat tcgcaaaata acaacacagg agggaggctt catgaacatc ttgtacattg
     2521 gtgatgttat ggggcaaccc ggtagaaaag cattggcaaa agttttgcca gggctaagga
     2581 tatctcgtga aattgatgta gtgattgctc aagcagaaaa cgttagtcac ggtaaaggta
     2641 tgagcctaaa acattacaga gagctagaag aaagcggcgt agatggcttt agtggtggta
     2701 accatagttt tgagcgtcca gatactgtaa agctcgtaca agacacgaac agtactgtcg
     2761 ttgcacctgc gaatgtaaaa ggtccactag aaaataacta taaaataata aaaaacgggg
     2821 taaaaaaggt ggcaatagtt tcgttacttg gctacacttt cccgcgcgga tacaatgaag
     2881 atactattaa cccacttatg tgcatagatg cacttttacc aaaaattcaa aaaaatatgc
     2941 cagatgccat tattgtaaat atccatagtg atttatcgag tgaaaaggtg atgatggggc
     3001 attacttaga tggtaaagtt actgctacgg tgggcgatca ttggcatatt ccaacagccg
     3061 atactcggct actaccaaag cacacagcgc acgttactga cgtaggtatg tgtggtgtgc
     3121 tcaattcttc acttggtgta gacgttgatg tcgcactgcg tagatggaag ggcgaacagg
     3181 ttaagaatgt aatggccaaa gaaaaagaac ttcagtttaa tgcagtactt attggtacgg
     3241 caccaggtag ccttatggca gatagtattg aacgtattca gtactatctg taagcaggct
     3301 tttattttca tctgtttacg cgtataatta ccctagtaaa attattttac ggggattggc
     3361 gcagttggct agcgcgcgcg gtttgggacc gcgaggtcga aggt
//
LOCUS       DAOPLS010000070         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_31362_length_3388_cov_3.215422, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000070 DAOPLS010000000
VERSION     DAOPLS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31362_length_3388_cov_3.215422"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2619)
                     /locus_tag="PKV52_02220"
     CDS             complement(<1..2619)
                     /locus_tag="PKV52_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPD99061.1"
                     /translation="MSKSSKKKSRLGLKTKSSSAKNKALDSANIVNRNIGDRMRARKD
                     LRARRRADQKARMPKNPIKRFVYYLKPKNFAAYWFNRDGAIRALKVAGVSLAVMMVFL
                     LATFAYFRKDLPRNITNLDTCSQGASSLYYDNTGQTLLWASSGDVECYPVKLENINTH
                     LQKAVIAIEDKDFYNHGGFSTAGVTRAFFNNLRGESTQGGSTITQQFVKNSLLSQEQT
                     YTRKVKELILAIELERSYTKDEILNAYLNEISFGSTYAGAEAAARGYFDKPAKDLTLD
                     ESATLAALIPAPSYYSPTGDNTGELVERRNYVLQLMVDQGYITQDEANTAKEQDTIAK
                     VVPKKSKFNNIIAPYFVLEAQKRLEEQYGATNIRKSGFKVTTTVDLRLQGYAEEAVKN
                     GMATIVRGGGDNAAMVAVDAQTGKVLAMVGGRDFEYPGFGQINYATTPRSPGSTFKPY
                     DYAALMTKSQNWGPGSILYDLKTNFGGGYSPDDYDKKQPGALSMRQSLGGSRNIPAIK
                     AMYMAGIPYVHDTAKKMGLTSGVTGCYTPGVEDCQEILSTAIGDGGQVRLDEHVNAFA
                     TFSRMGNYKPITYYTKVEDNKGKVIYEWTDPAGERALDEQVAYSINNILSDGAARYVG
                     LDNRVRINGVTTAVKTGTTNNSDNGWLMGFNTKIAAGVWVGHHENKTLSGFMEHKTAP
                     IWREFFAKANKDLAGAGDKWTQPAGMKRVCLSPTTGYAAKSGGKCDIFPSWYKPQYPD
                     STKSATIDSISNKLATECTPERAKQAITSGGIRAELPTSDPNYNNWMNPIKARYGGAG
                     GGLIPTDKDDIHTCDPADKPTVSLSEVQKKPDGSYSVTATIKKGKYPLTSVSFSINGT
                     VLEGGSYDISDSVSIPF"
     gene            2746..>3388
                     /locus_tag="PKV52_02225"
     CDS             2746..>3388
                     /locus_tag="PKV52_02225"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99062.1"
                     /translation="MTLHSPITDLKGVGPALASKFTKVGIATIGDFLQYVPRQYNDYS
                     GVVPIANMMPGNVTLQVTFTDVTQRRVKRGLHITQAIAQDSTGKVRIVWFNQPYRASQ
                     LTHEEYLLRGVYEYKNNRLQIVNPSVELLSSVQKDVTILPTYPERVGLKSHQLRKLFT
                     TLFAQDLQVTDVLPRDIVIKHGLLSAYDAYKTIHLPNSEHALLDAKRRLAFEEL"
BASE COUNT          884 a          758 c          691 g         1055 t
ORIGIN      
        1 aaaaggaata gacacgctgt ctgaaatgtc ataagacccg ccctcaagca cagtaccgtt
       61 aatagaaaag ctcacgctcg ttaacgggta tttacctttt ttaatcgtgg cggtcacgct
      121 gtaactaccg tctggctttt tttgtacttc gcttaagcta accgttggtt tgtcagcagg
      181 gtcgcaggta tgaatatcat ctttatctgt tggtataagt ccaccaccag cgcctccgta
      241 gcgtgcttta attgggttca tccaattatt gtagtttggg tcactcgtgg gcagctcggc
      301 gcgtatacca ccacttgtga tagcttgttt ggcacgttct ggtgtacact cggttgcgag
      361 tttattagaa atagaatcaa tcgtggctga ctttgttgag tcagggtatt gtggtttata
      421 ccagcttgga aagatatcgc acttaccacc gctttttgca gcatagcctg tcgttgggct
      481 aagacatact cgtttcatgc cagcaggctg cgtccattta tcgccagctc ctgctaggtc
      541 tttgttggct tttgcaaaaa actcacgcca gataggtgcg gttttatgtt ccataaatcc
      601 gcttagtgtt ttattttcgt gatgaccaac ccatactcca gctgcaattt tcgtattaaa
      661 gcccatgagc cacccgttat cagagttatt tgtggttcca gtctttaccg ctgttgtgac
      721 accatttatt cttactctgt tatctaaccc tacgtatcgt gctgcgccat cgctaagtat
      781 gttgttgata gaatatgcta cttgctcgtc tagggcacgt tcaccggctg gatcggtcca
      841 ttcataaata actttacctt tgttgtcctc tacctttgtg taatatgtaa tcggtttata
      901 gtttcccata cgtgaaaacg tagcaaaggc attaacatgt tcatctaagc gtacttgacc
      961 accatcacca atcgcagtag ataaaatctc ttggcagtct tctacgcccg gcgtgtaaca
     1021 acccgttacg ccgctggtga gccccatctt ttttgctgta tcgtgtacgt atggtatgcc
     1081 tgccatgtac atggctttaa tagcaggaat atttctagaa ccacctaatg attgacgcat
     1141 tgatagtgca cctggctgtt ttttatcata gtcgtctggg ctataaccac caccaaaatt
     1201 agtttttaag tcatacaaaa tactacctgg gccccagttt tgcgacttcg tcatcaaggc
     1261 ggcataatcg taaggtttga aggtagagcc tgggctacgc ggagttgttg cgtaatttat
     1321 ttggccaaat ccggggtatt caaaatcccg cccaccaacc atagctagta ctttacctgt
     1381 ctgggcgtct accgcaacca ttgcagcatt atcaccgcca ccacgaacaa ttgtagccat
     1441 accatttttg accgcttctt cagcgtagcc ttgcagtcta agatctaccg ttgttgttac
     1501 cttgaaccct gattttctaa tgttggtagc cccatattgt tcctctagac gtttttgggc
     1561 ttcgagcaca aagtaaggtg caataatgtt attaaactta ctcttcttag gtacaacctt
     1621 agcaattgta tcttgttctt ttgctgtgtt tgcttcgtct tgcgttatgt agccttggtc
     1681 taccataagt tgcaaaacat aattgcgacg ctctaccagt tcacccgtgt tatcacccgt
     1741 tggtgaataa tacgatggcg cagggataag agccgctaag gtagctgatt catctagtgt
     1801 taagtctttg gctggtttgt caaagtagcc tcgtgcggca gcttcggcac cagcataggt
     1861 agaaccaaat gagatttcat ttaaatatgc gtttaagatt tcatcttttg tgtacgaacg
     1921 ctcgagctca atagctaaga ttaattcttt tacttttctc gtgtatgtct gttcttgtga
     1981 taacaaagag tttttaacga attgctgtgt aatagtagaa ccaccttggg tgctttcacc
     2041 acgcagatta ttaaagaacg cacgggtaac cccagctgta ctaaagccac catggttata
     2101 aaagtctttg tcttctatag caataactgc tttttggaga tgggtgttaa tgttttctaa
     2161 tttaacaggg taacattcta cgtcaccaga gcttgcccac aacaaagtct ggccagtgtt
     2221 gtcatagtaa agtgaagatg caccttgcga acatgtatct agatttgtaa tgtttctagg
     2281 caagtctttc ctgaaatatg caaaagtagc caataaaaat accatcatta ccgcaaggct
     2341 tacacctgct acttttaaag cacgtatagc accgtctctg ttaaaccaat aagcagcaaa
     2401 gttttttggc ttcaaatagt acacaaaacg ctttataggg tttttgggca ttcttgcctt
     2461 ttgatcagca cgcctacgcg cccgcaggtc tttacgagct cgcatacgat caccaatgtt
     2521 tcggtttacg atattagcag aatcgagtgc tttattcttg gcgctcgaac tcttcgtctt
     2581 taagccaaga cgtgacttct ttttactgga tttactcata tacctgtata tttacctcaa
     2641 tatcaccatc tattatacca aaattaccta caatatacac gttaaaaagt tagtgctttt
     2701 taggtcactg cgcacctgtt gcgttgtgcg gtacactgta ggtatatgac acttcacagt
     2761 ccaataaccg atcttaaagg agtaggccct gcactggcga gtaagttcac taaagtaggc
     2821 attgctacta ttggagattt tttacagtat gtaccaaggc aatacaacga ttatagtggc
     2881 gttgtaccta ttgccaatat gatgccgggg aatgttacct tgcaggtaac ctttacagat
     2941 gtaactcaac ggcgggtaaa acgtggattg cacattacac aagcaattgc ccaagattct
     3001 actggtaagg ttcgcatcgt atggtttaat caaccatacc gcgcgagtca actaacccat
     3061 gaagaatatt tacttcgggg cgtatacgaa tacaagaata atcgtttgca aatagtaaat
     3121 ccttctgtag aactactaag tagcgtgcaa aaagacgtta ccattttacc aacgtaccca
     3181 gaacgagtag ggttaaagag ccatcaactg agaaaactgt ttactacact gtttgctcaa
     3241 gatttgcagg tcactgatgt tttaccgaga gatattgtaa tcaagcatgg attactgtcg
     3301 gcgtacgatg cctataaaac aattcacctc ccaaattcag aacacgcctt attagacgca
     3361 aagcgacgtc ttgcctttga agagctat
//
LOCUS       DAOPLS010000071         3325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_32202_length_3325_cov_3.660245, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000071 DAOPLS010000000
VERSION     DAOPLS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32202_length_3325_cov_3.660245"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..904
                     /locus_tag="PKV52_02230"
     CDS             221..904
                     /locus_tag="PKV52_02230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPD99063.1"
                     /translation="MLKNRKMVIIGALTIGVIAALGGGFFAWQSAQQDSSQTKSSLST
                     GATVPLNYQNAGGDSISLTPKQNTPKDPNALSVDSSPTTSTNLGSGTTRQQTTNTGST
                     ADTTNFSDYEQYKDKNEVFFGDIVLGKGAEAVKDSKLAITYKGYLTNGQLFDQSRTGN
                     NGQLEPLLFTLGAGQLIPGFEQGVAGMKAGGTRRVIIPPSLGYGPNAQGSIPANSVLI
                     FDIQLTAAQ"
     gene            925..2154
                     /locus_tag="PKV52_02235"
     CDS             925..2154
                     /locus_tag="PKV52_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPD99064.1"
                     /translation="MNKIWHKKPPHMHNANVEATLAYIDQHWADLLYKNTQDTGTLIG
                     LPHDYIVPAAQANAQFNFEEQYYWDTYFILLGLNDQKYSQLAKGMLENLVALYKRFHM
                     IPNASRLYFLSRSQPPLLTSMILLYKETFGCSGEWFDEMMAIAEDEYTTVWMSTKHPH
                     WRKIGQLNRYYDINALHDLAEAESGWDMTPRFDRKCLDYYPIDLNSLLYKYEKDFADW
                     YRTKNDLKTAALWDNKAQKRAQIVDELLWGHVRKFYFDYNFEDEKRGLTWSLAGFYPM
                     WVGMVDDKKAKELVKQLGKFEKDGGLSTTSRPMINLSLLFGSVKTQWAYPNGWAPLQW
                     IVIKGLERYGYTEDANRLAKKWLKTCTDWYATHGVLLEKYNVVTPTREPIKGVYPSQT
                     GFGWTNGIYVALARDYA"
     gene            2154..2315
                     /locus_tag="PKV52_02240"
     CDS             2154..2315
                     /locus_tag="PKV52_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99065.1"
                     /translation="MQEEVLPCIDKLAFDTKKQAQAAATVAMYQHGVRLKPYVCPHCG
                     LWHLASDHS"
     gene            2354..2650
                     /locus_tag="PKV52_02245"
     CDS             2354..2650
                     /locus_tag="PKV52_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99066.1"
                     /translation="MKHLFTKKQKTKKPKPVAIENYKVSYLDKRSKRYHRIVMTAFSG
                     LFGLVGVLYVFTSNAATGEVAPNNNNTLVVVAIVLGGLFITGLMVYFYIRSKQK"
     gene            2686..>3325
                     /locus_tag="PKV52_02250"
     CDS             2686..>3325
                     /locus_tag="PKV52_02250"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPD99067.1"
                     /translation="MSKFYVTTSIPYVNSVPHLGHAMEFIQADVLARYHRQIGDDVLF
                     TTGTDEHGSKIAEKAKELGVSVEDFVAENSGAFKALLSTLNITNDKFIRTTDEDHEKR
                     VKIVWQNLQKYIYKNLYVGLYCVGCEEFVSEKIAKQNNNVCPAHNRVYEQIEEENYFF
                     ALSKFTSQIKQSITSDEFAVIPVSRKHEIMHVLEEGLDDISISRSKDKLMSSR"
BASE COUNT         1083 a          640 c          708 g          894 t
ORIGIN      
        1 gaaggcttac agttgctgtc tattacaccc caactagcgc tcagtattgt cggaattatt
       61 gcgcttatta cgctgctggc tgggctaata cctgctttta aagcgagccg gctagaccct
      121 atagaagcac tgcgctacga ataaatcaaa caccaataac cttttatgtc atagctcatt
      181 tgactttatt tactatcatt attagcgtac aattcttata atgcttaaaa acagaaaaat
      241 ggtaataata ggagcactca ctattggtgt catcgcggcg cttggtggtg ggttttttgc
      301 gtggcaatcg gcgcagcaag attcgtcgca aacgaaaagc tcgctcagca cgggtgctac
      361 cgtacctctt aattaccaga atgcaggagg tgattctatt agtcttacac cgaaacagaa
      421 tactcctaaa gatccgaacg cgctaagcgt tgactcttca ccaactacga gcactaactt
      481 aggttcaggt accacgagac aacagacaac gaatactggt agtaccgctg acacgacaaa
      541 ttttagcgac tatgaacaat acaaagataa aaatgaagtt ttttttggtg atattgtttt
      601 aggcaaaggc gccgaggccg ttaaagactc taaacttgcc attacctata agggttactt
      661 aacaaacggt cagctatttg atcaatcacg cacaggcaac aatgggcagc tagaaccact
      721 actgtttacg cttggtgctg gccaactgat acctggtttt gaacaaggcg ttgcaggcat
      781 gaaagcaggt ggcacgaggc gcgttataat accacccagc cttggctatg gtccgaacgc
      841 acaaggcagt ataccggcga attctgtatt aatatttgat attcaactta ccgcagcaca
      901 ataaatctaa ataggtagat tgttatgaat aaaatttggc ataaaaagcc accacatatg
      961 cataatgcga acgttgaagc gacgcttgcc tacatagacc aacactgggc tgatttacta
     1021 tataaaaaca cacaagacac aggcacgtta ataggattac cgcacgacta catagttccg
     1081 gctgcccagg cgaatgcgca atttaatttt gaagaacagt actactggga tacttatttc
     1141 atactgctgg ggcttaacga ccaaaaatat agtcaactcg ccaaaggaat gttagaaaat
     1201 ttagtagcac tgtataaacg gtttcatatg attccaaacg ctagtcggct ctatttttta
     1261 agtcgttcac aaccaccact gctcacgtct atgatacttt tatataaaga aacatttggt
     1321 tgttctggtg agtggtttga tgaaatgatg gcgatagctg aagatgaata tactaccgtg
     1381 tggatgagta cgaagcatcc acactggcga aaaattgggc agcttaatag atactacgat
     1441 atcaatgccc tccatgatct ggccgaagct gaaagtgggt gggatatgac accacgcttt
     1501 gatagaaagt gtctagatta ctatccgatt gacctaaatt cattactata taagtatgaa
     1561 aaagattttg ctgattggta tcggacaaaa aacgatctaa aaaccgctgc attatgggac
     1621 aataaggccc aaaaacgtgc ccaaattgtt gatgaattat tatggggtca tgtgcgaaaa
     1681 ttttattttg attataactt tgaagatgag aaaagaggct taacgtggtc acttgctggg
     1741 ttttacccaa tgtgggttgg catggtagat gataaaaaag cgaaagaact tgtaaaacaa
     1801 cttggtaagt ttgaaaaaga tggtggttta agcaccacat cgcgtccaat gattaattta
     1861 tcattgctgt ttggatcggt gaaaacacag tgggcgtacc caaatggctg ggcaccactg
     1921 cagtggattg ttattaaggg tctcgaaaga tacggatata cagaagacgc aaaccggctc
     1981 gcgaagaaat ggcttaaaac atgtactgat tggtatgcca cccatggcgt actattagaa
     2041 aaatataatg ttgttacccc aacaagagag cctataaaag gcgtataccc gagccagaca
     2101 ggatttggct ggacgaatgg aatatatgtt gcattggcaa gagattacgc gtaatgcaag
     2161 aagaagtgct gccatgcata gataaattag cctttgacac aaaaaaacaa gcccaagctg
     2221 ccgcgacagt tgctatgtat caacacggcg tacgccttaa gccctatgtg tgcccgcact
     2281 gtggcttgtg gcatttagct tcagatcaca gctaatgcta tactgcaaag gatagtagta
     2341 aaaaacaaac catatgaaac acctatttac taaaaaacaa aaaacaaaaa agccaaaacc
     2401 agtggcgata gaaaactaca aagtatcata tttagataaa cgatcaaaaa ggtatcatcg
     2461 tatcgttatg actgcttttt ctggactttt tggattggta ggtgtgttgt atgtatttac
     2521 gtctaatgct gctacaggag aagttgcacc caacaacaac aatactctgg tggtagtggc
     2581 tattgtttta ggcggtctct ttataaccgg tttgatggta tatttttaca tacgcagtaa
     2641 acagaagtag cgcatagttt tgtaaaactg ttacaatgaa cagatatgag taaattttat
     2701 gtcaccactt ctattcccta tgtaaactcc gtgccacacc ttggccatgc tatggagttt
     2761 atacaagctg atgttttagc ccgataccat cgtcagatag gcgacgatgt gctatttacc
     2821 actggcaccg atgaacatgg tagtaaaata gcagaaaaag ccaaggagct tggtgtatcg
     2881 gttgaagatt ttgtagcaga aaatagcggg gcttttaagg cgctacttag tacactcaat
     2941 atcacaaatg acaagtttat tcgtacaact gatgaagatc acgaaaaacg ggtaaaaata
     3001 gtatggcaaa acttacaaaa gtatatctac aagaacttat acgttggttt gtattgtgta
     3061 ggctgtgaag agtttgtgtc agaaaaaatt gccaagcaaa ataataatgt ttgcccagca
     3121 cataatcgcg tctacgagca gatagaagaa gagaattatt tttttgcgct gagtaaattt
     3181 accagccaaa taaaacaatc tatcacaagc gacgaatttg ctgtcatacc tgtttcgcgt
     3241 aagcacgaaa tcatgcatgt tcttgaagaa ggtctagatg acattagcat atctagatct
     3301 aaagataagc taatgtcatc tagac
//
LOCUS       DAOPLS010000072         3305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_32449_length_3305_cov_4.046462, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000072 DAOPLS010000000
VERSION     DAOPLS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32449_length_3305_cov_4.046462"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..708)
                     /locus_tag="PKV52_02255"
     CDS             complement(151..708)
                     /locus_tag="PKV52_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018809598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPD99068.1"
                     /translation="MERTLIILKPDTIKRAIAGEILTRFEKAGLKIIGLKMMTPSQDL
                     VTKHYPDALVPIVGNKTKKDWDNYGIDYKETAEEIGEMIVTATREFMRSSPVIAAVLE
                     GGHAVEVVRKMVGSTGPKDSAPGTIRGDYAHLSLGRASLKKKGAANLIHASGTVEEAE
                     KEIAMWFDDSELFEYETVHEVLTRV"
     gene            complement(731..1528)
                     /locus_tag="PKV52_02260"
     CDS             complement(731..1528)
                     /locus_tag="PKV52_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPD99069.1"
                     /translation="MSYLDAIILGLVQGLTEFIPVSSSGHLVIFNYLLGTPESFAFDV
                     LLNIGTLSALILFYRKRIKELIIRTFVGKEWALLSKLLAATIPAFAIGLSFGNQIKAL
                     NNMVWVVVIMLVLVGILMIIYGKPNPESDDSPLESSVTWPVALRVGFAQAIALIPGTS
                     RSGITILTGLRSNLSAARAAEFSFMLAIPTIAGATTKVMLLDGGWSYVTSNVGVVLVG
                     NIVSFASGLFAIGFLLKLLSTRGLRDFGWYRIGLAAVLSVLLVTGII"
     gene            complement(1630..2205)
                     /locus_tag="PKV52_02265"
     CDS             complement(1630..2205)
                     /locus_tag="PKV52_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99070.1"
                     /translation="MKNKKYTNIKKITNITMRAFVKTAGAAGAAGIIVVAPNATQAIT
                     LVAKKLNKKQPKRFSEQVKNSGYFSVQKIDDNRYAITLTPKGQSINTKVLFEDYKIPI
                     NGAWDNKWHLLMFDIPEQYRLVRDTLRYKIESLGMVLIQNSVYVYPYDISEFVAMVHT
                     NYPFASKHIISAEVTHLDGQNQLIQKFKSIL"
     gene            2281..3192
                     /locus_tag="PKV52_02270"
     CDS             2281..3192
                     /locus_tag="PKV52_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPD99071.1"
                     /translation="MDLETLIGDYLEYMAIERGRSKKTIANYSHYLTRLVDFAGDVTI
                     DEVTPDLIRKWRLWLNDLGSDRSDELGKATQNYHLIALRNFLKYCHKRDIAAMSADQI
                     ELAKVARTKVTFLDKDELERLLEQPNTQEIAGLRDRAILELLYSSGLRVSELVSLDTA
                     HINLKRREFTVRGKGQKDRPVFISQQAADWVQLYIDTREDALPALFISYGGKAATNTT
                     GNYRRLTPRSIQRMVARYALLAGITKHVSPHSLRHSFATDLLMNGADIRSVQAMLGHS
                     NIATTQIYTHVTDPHLRAVHEKFHNKA"
BASE COUNT          960 a          754 c          616 g          975 t
ORIGIN      
        1 agcttaggga ttttctcgga aattggttcg gacttcggcg taaaatcgca ccattacaaa
       61 atacctcgct ctatggcgag gtattttgta atcataactt tcgtttgtgt ctagcgtacg
      121 tataaatgta acttacaaac tattttttac ctacactcgg gttaacactt cgtgaacggt
      181 ttcgtattca aatagttcac tatcgtcaaa ccacatggca atttcttttt ctgcttcttc
      241 tacagtccca gaagcatgta ttaaattagc agcacctttc ttttttaacg atgccctccc
      301 tagacttaaa tgagcataat cgcccctgat tgttcctgga gcagaatctt ttggaccggt
      361 actgccaacc atttttctaa caacctctac cgcatggccg ccttcgagca cggctgcaat
      421 aactggcgag ctcctcataa attctctggt tgctgtcaca atcatttcac caatttcttc
      481 ggcagtttct ttataatcta taccgtaatt atcccagtct ttttttgttt tattaccaac
      541 aataggcaca agtgcgtcag ggtaatgctt cgttacaagg tcttggcttg gagtcatcat
      601 tttaaggcca attattttta agcctgcttt ttcaaatcgt gttaagattt cgcccgcaat
      661 cgctcgtttt attgtatccg gctttaatat tatgagtgtt cgttccatat ctatcctttc
      721 atttgcttgt ctatataata ccagtcacaa gcaatactga taagactgcc gctaaaccaa
      781 tacggtacca accaaaatct cgcaaacccc gcgtactaag taacttaagt aaaaagccta
      841 tagcaaatag gccactcgca aacgaaacaa tgttaccaac aagtacaaca ccaacattag
      901 acgttacgta tgaccagcca ccatctagaa gcattacttt agttgtagcg ccagctattg
      961 tgggtattgc taacataaag ctaaattcgg cagctctagc ggcgctaaga ttactccgta
     1021 atcctgtcag aattgttata ccactacgtg acgtaccagg tataagcgct attgcttgtg
     1081 caaaaccaac ccgtagtgcc actggccacg taaccgaact ttctagcgga ctatcgtctg
     1141 attctgggtt aggcttaccg tatataatca tcaaaatacc tactagaacc agcattataa
     1201 caacgaccca caccatatta ttgagcgctt ttatttgatt accaaacgac agaccaatcg
     1261 caaaagccgg gatagttgca gccagcaatt tactcaggag ggcccattct ttaccaacga
     1321 atgttcgtat gatcaattct ttgatacgtt tgcgataaaa caatataagc gcactgagcg
     1381 tacctatgtt tagtagtacg tcaaacgcaa aagattcggg tgtaccaagc aagtagttaa
     1441 atatcacaag atgccccgaa cttgagaccg gaataaactc tgttagtcct tgaactaacc
     1501 ccaaaataat tgcatctaaa tagctcatct tgttagcact tatgctacca cactacccca
     1561 tacatcccaa gtcacacacc aaccccatta cacatatatt tacgccgacc gtcgtaaata
     1621 tatgtgtaat tataatatag atttaaattt ttgtatgagc tggttttggc cgtctaggtg
     1681 cgtaacttct gcactaataa tgtgtttact cgcaaatggg tagtttgtgt gcaccatagc
     1741 tacaaattcg ctaatatcgt atgggtagac gtatacgcta ttttgaatta ataccatccc
     1801 taaagattca attttatagc gtagcgtatc ccgcactaat ctatattgtt caggaatatc
     1861 gaacattaat aaatgccatt tattgtccca agctccatta atagggattt tgtaatcttc
     1921 aaatagcacc ttggtattta tactttgccc ttttggtgta agcgttattg catacctatt
     1981 gtcatctatt ttttgaacac taaaataacc gctatttttt acttgttcac taaatcgttt
     2041 gggctgtttt ttgttgagct tcttagcaac caatgtaata gcctgtgtcg cattaggcgc
     2101 tacaacaata atacccgccg ctcctgcagc accagctgtt ttaacaaaag cgcgcattgt
     2161 tatattggta atctttttta tgttcgtata tttcttattc ttcataatac atatattata
     2221 cacgaccgtc gtaaatatat gtgtttttga gggatggggg gaatgtcgta caatataagt
     2281 atggatttag aaacgctcat tggggattat ttagaatata tggctataga gcgtggccgt
     2341 tcaaaaaaaa caattgccaa ctatagccac tacctaacgc gtttggttga ttttgcaggt
     2401 gacgtaacaa tagatgaagt aacgcctgat ttaatacgaa aatggcgcct gtggctaaat
     2461 gaccttggct ctgataggtc tgacgagctt ggtaaggcga cacaaaatta tcacctaatt
     2521 gccctgcgca actttttaaa atactgccat aaacgtgata ttgcagcgat gagcgccgac
     2581 caaatagagc tcgctaaagt agcgcgcacc aaagttacct ttttagacaa agacgagtta
     2641 gaacggttgc tagagcaacc caacacacaa gaaatagctg gtttgcgcga tagagcgata
     2701 ttagaattac tgtattctag cggtttgcgc gtttctgaac tagtgagtct agatacagcg
     2761 cacataaact taaagcgccg tgaatttacc gtacgtggta aaggccaaaa agatcgccca
     2821 gtgtttataa gccagcaagc ggctgattgg gtgcaacttt atatagatac gcgtgaagat
     2881 gcattacctg ccctgtttat tagctatggg ggcaaagcag caaccaacac aactggtaac
     2941 taccgtcggc tcacaccacg cagtattcag cgtatggtgg cgcgctacgc cctgctagcg
     3001 ggcattacca agcatgtgag cccgcacagc ttgcggcata gctttgctac cgacttactc
     3061 atgaacggtg ccgatatacg aagcgtgcaa gccatgcttg gccatagtaa cattgctacc
     3121 acccaaattt atacccacgt taccgaccca cacctaagag ctgtccacga aaagttccat
     3181 aataaagcct aattacggcg caggacaagc gccaccacct gatatgttaa agttattagc
     3241 gccatctact ttgatgtctt tacacaaact atcagctaat gctccaaaat atggttgcca
     3301 cgtgc
//
LOCUS       DAOPLS010000073         3282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_32768_length_3282_cov_4.018593, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000073 DAOPLS010000000
VERSION     DAOPLS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32768_length_3282_cov_4.018593"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..2493
                     /gene="gyrA"
                     /locus_tag="PKV52_02275"
     CDS             7..2493
                     /gene="gyrA"
                     /locus_tag="PKV52_02275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPD99072.1"
                     /translation="MEIIPAQTHSREVELTSVENIMEDSYLRYSMSVIVSRALPDVRD
                     GLKPVHRRILYTMNLEGLRAGARHRKSATVVGSVMGDYHPHGDSAIYDSMVRMAQDWS
                     MRYTLVDGQGNFGSMDGDPPAAMRYTEARMARVAEEMLVDIDKETVDWRDNYDGRKQE
                     PTVLPARLPNLLLNGQIGIAVGMATSIPPHNLGEIIDAITHLIDTPEASTDDLLQYVK
                     GPDFPTGGIVYGSESLTTAYKTGRGGVVVRGVADIQERSKKGAFRIVISEIPYNLNKA
                     SLIEKIADLVKDKKITGISDLRDESARGIVRIVVDLKKDAYPKKVLNQLYKLTPLQTS
                     FHYNMLALVDGIQPRVLSLSDILHEYIKHRQVVVRRRTEFELKKAEARAHILEGYKVA
                     LDNIDEVIRVIRASKTVEEASKNLMQQFKLSEIQAKSILALPLRTLTGLERQKIEDEL
                     AELIKLITNLKAILADETKILGIIKQELADLKERYGDERRTKIVSQELGKFSDEELIP
                     DEQVVVTLTTENYIKRSPAVDYKRQGRGGKGRRGMTTRDEDLIEHLVIASTHDFLLFF
                     TNKGRVFRLKGYEIPATGLNAKGVAIVNLLQLQPEEKVSALIRVSKSEENGYLFMATV
                     RGVVKKTAYEQYRNVRTSGLIAINLDDGDELKWIRFSTGDNEVVISTSLGQAIRFHEK
                     EVRAMGRVSRGVRGIRLRPNDYVIGMDIVHEDSNIFVISELGYGKRTKVAQFTAHKRG
                     GVGIRSAVVNKKTGNLIGVANLTEEANEVIIISSQGQTIRLGIKDISELGRATQGVRI
                     MRLNNGDSVVSLALVAKVLQEVEETADDSE"
     gene            2486..2905
                     /locus_tag="PKV52_02280"
     CDS             2486..2905
                     /locus_tag="PKV52_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HPD99073.1"
                     /translation="MNKYAILVAPFAGWIIAQLLKFIFTLRKDGIQFSDAIQSGGMPS
                     SHAAFTSSLTTAVGYQYGISSAYFAIAVAFTAIVMYDAMGVRRTTGEQTKALQQLAKK
                     TNIKLAVLHDARGHSPVEVLAGLVVGIITGLLTVVLL"
     gene            3225..>3282
                     /locus_tag="PKV52_02285"
     rRNA            3225..>3282
                     /locus_tag="PKV52_02285"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1001 a          612 c          767 g          902 t
ORIGIN      
        1 ccagatatgg aaattattcc tgctcaaaca cattcaagag aagtagaact tacctctgtt
       61 gaaaatatca tggaagatag ctacttacgc tactctatga gtgtcattgt gtcgcgtgca
      121 ttacctgacg tacgtgatgg acttaagcct gttcatcgtc gtattttata caccatgaac
      181 ctcgagggtc tacgtgccgg agcacgtcat cgtaaatccg caacggtggt aggttctgtg
      241 atgggcgatt accacccgca tggcgattct gccatttacg attctatggt acgtatggct
      301 caagattggt ctatgcgtta cacattggtt gatggccaag gtaactttgg ctctatggac
      361 ggagatccgc cagcagccat gcgatacaca gaagcccgta tggctcgggt cgcagaagaa
      421 atgctcgtag atatagataa agaaacggtt gactggcgtg ataactatga cggccgcaag
      481 caagaaccaa ctgtcttacc cgcacgttta ccgaatcttc ttttaaacgg acagattgga
      541 atagcggtag gaatggctac gtctatacct ccacataact tgggtgaaat tattgatgcg
      601 attacgcatc ttatagacac accagaagca agtaccgacg atctattgca atatgtgaaa
      661 ggtcctgatt tccctacggg tggcatagtg tacggtagtg aatcgttaac aactgcttat
      721 aagactggcc gtggtggtgt ggttgtcagg ggtgtggcag atattcagga gcgttctaaa
      781 aaaggtgcat tcagaattgt tatatcggaa attccttaca atctaaacaa agcgtcactt
      841 atagagaaaa ttgctgattt agtaaaagat aaaaagatta ccggcatatc tgacttacga
      901 gacgaaagtg cccgcggtat tgtgcgtatt gtggtagacc tgaaaaaaga tgcataccct
      961 aaaaaagtat tgaatcaatt atataaactt actccactgc aaacatcgtt tcattacaat
     1021 atgctcgcgt tagttgatgg tattcaacca cgagttctga gccttagtga tatattgcac
     1081 gaatatatta agcatcgcca agttgtcgta cggcgcagga ctgaattcga gctcaagaag
     1141 gcagaggctc gtgcccacat actagaagga tataaagtag ccttagataa tatagacgaa
     1201 gtaattcgtg tcatacgtgc ttctaagacg gtagaagaag cttccaagaa ccttatgcag
     1261 caatttaagc tgtctgagat tcaggcaaaa tctatcttag cgttgccact tagaacacta
     1321 acaggtctag agcgacaaaa aattgaagat gaacttgccg aacttatcaa acttattact
     1381 aacctcaagg ctattttggc agatgaaacc aagatattgg gtattatcaa gcaagaactt
     1441 gccgacctta aagagcgcta cggtgatgag cgacgtacca agatagtctc tcaagagctt
     1501 ggaaagttta gtgacgaaga actaattcct gatgaacaag tagtagtaac tctaacaaca
     1561 gaaaactata ttaaacgaag cccagctgta gactataaaa gacaaggtcg cggtggtaag
     1621 ggtcgtcgcg gtatgacaac cagagatgaa gatcttatag aacatcttgt tattgccagc
     1681 acgcatgact ttttgctgtt ctttaccaat aaaggaaggg tattccgact aaaaggttac
     1741 gaaattccag caactggcct caatgcaaag ggcgttgcta ttgtaaacct attgcagtta
     1801 cagcctgaag aaaaagtaag tgcacttata agggtaagca aatcagaaga gaacggatat
     1861 ttgttcatgg caacagtacg gggtgttgta aagaaaactg catacgaaca gtacagaaat
     1921 gtacgcacct ctgggctcat agcaattaac ctagatgatg gtgatgaatt aaagtggatt
     1981 cgctttagca caggagataa cgaagtagtt atttctacat cgttaggtca ggcgatacgg
     2041 ttccatgaaa aagaagttag ggctatgggt agagtatcta ggggtgtacg tggaatacgt
     2101 ttgcgcccga acgattacgt tatcggcatg gatatagttc acgaagattc aaatatattt
     2161 gttattagcg aactagggta tggcaagcgt acgaaggttg ctcagtttac tgctcataaa
     2221 aggggtggtg taggtattag atcagcggtt gttaacaaaa aaaccggtaa tctaattggt
     2281 gtcgccaatc ttacagaaga agcgaatgag gtgattatca tttcttctca agggcaaact
     2341 atacgtttag gcattaaaga tatatcagaa ctcggtcgcg caactcaagg tgttcgtatc
     2401 atgcgtttaa ataacggtga tagtgttgtt tctcttgcat tggtagcgaa ggtcttacaa
     2461 gaagtagaag agacggcaga cgacagtgaa taagtatgct attttagttg ctccttttgc
     2521 tggctggata atagctcaat tattaaaatt catttttacc ctaagaaaag atggtattca
     2581 atttagtgat gcaattcaat ctggtggcat gccaagttcg cacgctgcgt ttaccagttc
     2641 tttgactaca gccgtggggt atcaatatgg aatcagttct gcctattttg caatagctgt
     2701 agcatttact gcaattgtca tgtacgacgc tatgggggta agacgtacta ctggcgagca
     2761 aaccaaggcg ctgcagcagc tagcaaaaaa gacgaatatt aagcttgcag tactgcatga
     2821 tgctcggggg cactcaccag tagaagtctt agctggtctg gttgttggga tcatcaccgg
     2881 actgctaaca gtagtattat tgtagaaaaa tctactacat ttaatgtccg tacatgcttg
     2941 acccggcggt ggatttagag tagtattact aaagatattt tgaggcgcat tgtttaatgt
     3001 gctcaaaaaa tcggacgtta aaaattgaat agtgcagcgt caacgtcaag ttgataagta
     3061 aatttctttt taagtaattt caagataagt cagcgcagat tttttaaatc tgttctttaa
     3121 tggagagttt gatcctggct catgatgaac cctagaaggt tttatgacgg catccgccgc
     3181 cagcgaattt tttagaacta attatgtgtc actcaacctc cactttaatg gagagtttga
     3241 tcctggctca ggatgaacgc tggcggcgtg cctaatacat gc
//
LOCUS       DAOPLS010000074         3275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_32867_length_3275_cov_3.468634, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000074 DAOPLS010000000
VERSION     DAOPLS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32867_length_3275_cov_3.468634"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /gene="ppsA"
                     /locus_tag="PKV52_02290"
     CDS             <1..1846
                     /gene="ppsA"
                     /locus_tag="PKV52_02290"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPD99074.1"
                     /translation="SNGFDHMKVALSVGIQQMVAVRSECAGVLFTIDTESGFKNTVVV
                     SSIYGLGENIVQGHVNPDEFIVFKPTMAILKRHLGTKKMKMVPIGANRTKNLPVKVSD
                     QNRFSINDDQVKTLAHWGMLIEQHYGHPMDIEWALDEDDGQLYIVQARAETVQSRRDT
                     NIIEEYRLAQEGHVLATGTSVGSKIGTGKVSKIMSVKDIDDFKEGDVLLTEMTDPDWV
                     PIMKIASAIVTDKGGRTCHAAIVSRELGIPCVVGTGNASELIHDGQDVTVSCAGGSDE
                     GAVYEGILHYEVDKTDVKDLPRTKTKIMMNIGSPDHAFAYSQIPNDGVGLAREEFIID
                     SHIKIHPLALLRFNEVKDKAVRAQINQLTLGYEDKAQYFIDKLAEGIATIGAAFYPND
                     VIIRFSDFKTNEYANLIGGTQFEPQENNPMIGWRGASRYYSEQYRAAFELECQALKKV
                     RDEMGITNVKAMIPFCRDLEEARKVLEIMEYCGLKRGENGFEVYVMVEIPSNVLLARE
                     FAKLFDGFSIGSNDLTQLTLGVDRDNHEVAHVYSENNEAVKALIRSVVAVAKETNTKI
                     GLCGQAPSDYPEFARFLVETGIDSISLAPDTVVKTTIDIKKTEESLGR"
     gene            1871..>3275
                     /gene="pyk"
                     /locus_tag="PKV52_02295"
     CDS             1871..>3275
                     /gene="pyk"
                     /locus_tag="PKV52_02295"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPD99075.1"
                     /translation="MQSIVPKKPIRQYKRTKIIATCGPSSLEYDTIHKLIAAGANGIR
                     LNFSHGNHAQHATTIKRVRKASKALGKPVAIIQDLQGPKVRLGEFDGIFQVEKGQSIR
                     LRYNADFEREGVLPTQYDLSKKVKRGERLYFFDGKVKTTITSVKDGVVHVRAENAGYL
                     LARKGINVPDTNFDGDIITKKDKEDILFGATGDIDYVALSFVQTASDVAHVKRLLTNA
                     GSKARVISKIETRSAVENIEAIVEASDAVMVARGDLAIETSVEEVPILQRQIVRLGIT
                     HAKPTIIATHMLASMTDAPQPTRAEASDVATAVLSGTDAVMLSEETANGMFPIDAVKT
                     MKKIIRYAEENTHENITFNRTQQSHTRQTAISKAIIALAYDVSAIAIVAETKSGATGY
                     IIASYRSDLPIIAVTSSQLVAQQLSIVYGIKSFVRKDEMYQAVHLTDWLQRNKVLSKN
                     EIVVIVSGEHPGVVGTTD"
BASE COUNT         1065 a          606 c          765 g          839 t
ORIGIN      
        1 atctaacgga tttgaccaca tgaaagtagc gctttcggtt ggtattcaac aaatggtagc
       61 agtgcgcagt gaatgtgctg gagtactttt taccatcgat actgaatctg gatttaaaaa
      121 taccgtagtt gtgagttcaa tttacggctt aggagaaaac attgttcaag ggcacgttaa
      181 ccccgatgag tttattgtat ttaagccaac tatggctatc ctaaaacgac atcttggtac
      241 aaaaaaaatg aagatggtac caattggtgc taatcgtacc aaaaacctgc ctgtaaaagt
      301 aagtgatcaa aaccgttttt ctataaacga tgatcaagta aagacgctgg cgcactgggg
      361 aatgcttatt gaacagcatt acggtcaccc aatggacatt gaatgggctc tagatgaaga
      421 cgacggtcaa ttgtacattg tgcaggccag ggcagaaacg gtacagtctc gccgcgacac
      481 gaacattatt gaagaatatc gtttagctca agaaggtcac gtgctggcaa ctggtacgag
      541 tgtaggtagc aaaatcggta ctggtaaagt aagtaaaatt atgagcgtca aagacattga
      601 cgattttaaa gagggtgatg ttctgcttac agaaatgacc gacccagact gggtgcctat
      661 tatgaagata gcgagtgcta ttgttaccga taaaggtggg cgtacctgcc atgcagccat
      721 tgtatcgagg gaactaggca ttccatgtgt tgttggcaca ggcaacgcct cagaactcat
      781 acatgatggt caagatgtaa cagtaagctg tgctggcggt agtgacgaag gcgctgtgta
      841 cgaaggaatt ttgcactatg aggtagataa aacagatgtt aaagacttac cacggacaaa
      901 aaccaagatt atgatgaaca ttggttcacc tgaccatgcg tttgcatatt cgcaaattcc
      961 taatgatggt gttggcttag cgcgagaaga atttatcatt gattcgcata taaagattca
     1021 ccccttagca ttattgcgat tcaacgaagt taaagataaa gcggtacgtg ctcagataaa
     1081 tcagttaacg ctcggttacg aagataaagc acaatacttt atagataaat tagccgaagg
     1141 tatcgctaca ataggtgccg cattttaccc caatgacgtt attattcgtt ttagtgactt
     1201 caagacaaat gaatatgcta acttaattgg tggtacacag tttgagccgc aagaaaacaa
     1261 tccgatgatt ggatggcgtg gcgcgagtcg ttactatagc gagcaatacc gagctgcctt
     1321 tgagctagag tgtcaggcgc taaaaaaagt tcgtgatgaa atgggtatta caaacgttaa
     1381 agcgatgatt ccattttgcc gtgaccttga agaggcgcgc aaggtgttag agattatgga
     1441 gtattgtggt ctcaaacgtg gtgaaaatgg ttttgaagta tacgttatgg ttgaaatacc
     1501 atctaacgta ctattggccc gtgaatttgc gaaactattc gatggatttt caattggttc
     1561 taatgacctt acacaactga ctttgggtgt agacagggat aaccacgaag tcgctcacgt
     1621 ttatagcgaa aacaacgaag cagtaaaagc cttaattcgt agcgttgtgg cggtggcaaa
     1681 agagaccaac acaaaaattg gtttatgtgg tcaagcacct tctgattacc ctgagtttgc
     1741 acgctttttg gtagaaacag gcatagatag tatttcctta gctccagaca ctgtcgttaa
     1801 aacaactatt gatattaaga aaaccgaaga atcacttggt agataaacga atcaagtaat
     1861 ctataataca atgcagagta ttgttcctaa aaagccaatt cgccagtata aacgtacaaa
     1921 gataattgca acgtgtggtc ctagttcact agaatatgac acgatacata agcttatcgc
     1981 tgcaggcgct aatggaatac ggcttaattt tagtcatggc aaccatgcgc aacacgcaac
     2041 gacaattaag cgtgtcagaa aagcatctaa agcactcggt aagccagtgg cgattattca
     2101 agatttacaa ggtccaaaag tacgtttagg tgaatttgat ggcatatttc aggtagaaaa
     2161 aggtcaaagt attcggctgc gttacaacgc tgattttgag agagaaggcg tattacctac
     2221 acaatacgat ttaagtaaaa aagtgaaacg tggcgaacga ttgtactttt ttgacggaaa
     2281 agtaaaaaca accattacga gtgtcaaaga cggagttgtg catgtacgtg ccgaaaacgc
     2341 agggtattta ctcgcccgca aagggatcaa tgtaccagat accaattttg atggcgatat
     2401 cattaccaag aaagacaaag aagatatctt gtttggagca acgggcgaca tagattatgt
     2461 tgcactgagc tttgtgcaaa cggccagtga cgttgctcac gtgaaacgtc tacttaccaa
     2521 tgctgggagt aaggcgcgtg ttatctctaa aatagaaaca cgttctgctg tagagaatat
     2581 agaagctatc gttgaagcgt cagatgcagt tatggtagcg cggggcgatt tagctataga
     2641 aacaagcgta gaagaagtcc ctatattgca aagacaaatt gtgaggcttg gcattacaca
     2701 tgcaaaacct accataattg caacgcacat gcttgcaagt atgacagacg caccacagcc
     2761 aacacgagca gaggcgagtg atgtagcgac agcagttctc agtggtaccg atgcagtcat
     2821 gttaagcgaa gaaacagcca atggaatgtt tcctatagat gctgttaaaa cgatgaaaaa
     2881 gattattcgc tacgcagaag agaatactca cgaaaacatt acatttaacc gtacacaaca
     2941 atcgcacacg cgccagactg ctataagtaa agcaattatt gccttagcgt acgatgttag
     3001 tgctatagct attgtagcgg agacaaaaag cggtgcgaca gggtacatta ttgcgtcgta
     3061 tcggtcagat ttaccaatta ttgcagtgac aagtagtcag ctggtagctc aacaactatc
     3121 tattgtgtat ggcataaaga gtttcgtgcg caaagatgaa atgtaccaag cagtacattt
     3181 aacagattgg ctacaaagaa acaaagtact ttcaaagaac gaaatcgttg taattgtatc
     3241 aggcgagcat cctggcgtcg taggcacaac agaca
//
LOCUS       DAOPLS010000075         3266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_32985_length_3266_cov_4.246029, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000075 DAOPLS010000000
VERSION     DAOPLS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32985_length_3266_cov_4.246029"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKV52_02300"
     CDS             complement(<1..726)
                     /locus_tag="PKV52_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPD99076.1"
                     /translation="MWLIVLGLFLFVLLIVLHEYGHFLVAKRNGVDVEEFGIGFPPKL
                     FGKKLGKGIFEGYYTINLLPLGGFVRLKGEEDAATEKGSFGSVGLSAKLRIMLAGVAV
                     NLLAALVFFTIVAWVGMPQVIPNQFSVPSDSTVLRNDIFAGYVDADSPAAQAGLQVGD
                     TIVSLNNQPITNEASVREVASANAGNTVPIVYERDGQTTTATTTLLSEQQVADSKNTD
                     TPKGYLGVVPREYTLIQSTWSAPI"
     gene            complement(726..1415)
                     /gene="uppS"
                     /locus_tag="PKV52_02305"
     CDS             complement(726..1415)
                     /gene="uppS"
                     /locus_tag="PKV52_02305"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPD99077.1"
                     /translation="MVHNDTQPQHVGIVLDGNRRWAKQHGVSAQEGHKQGFETFRRIA
                     DAGLARGIPYLSVFAFSTENWNRAKDEVSFLMRFIQIVMNKYISELTKKDIRFIWLGS
                     EEGLDAKIIKQLRRAEESSKNNTTATFCLCFNYGGQQEIADAAAKLAAMGTPITQESI
                     ETALYGGSAVPPVDLLIRPSGEMRISNFMLWRAAYSELFFTDKLWPDFTPEDLDEALA
                     AYASRHRRFGA"
     gene            complement(1471..2034)
                     /gene="frr"
                     /locus_tag="PKV52_02310"
     CDS             complement(1471..2034)
                     /gene="frr"
                     /locus_tag="PKV52_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPD99078.1"
                     /translation="MSPNQLVEDAKTKLATAVNHFNDEIKKLRTGRAHPSMLDGITVL
                     AYGTPMPLNQVSNVTAPEAQQLQITPFDPSNIQAISDAIRNDQSLGLNPADDGRVVRI
                     SIPPLTTERRQQIVKQLNEKTEETHIACRNIRHEALDSAKQAKADKDISEDDYTRIEK
                     QVEDLMTKTKQDIETATKAKEQEIMTV"
     gene            complement(2115..2711)
                     /locus_tag="PKV52_02315"
     CDS             complement(2115..2711)
                     /locus_tag="PKV52_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPD99079.1"
                     /translation="MAIDIDTIKRLRELTGVGITDAKKALVEAEGDFDKALAAMRKKG
                     MTKAEKRGEREARAGVIGSYLHDGRIGVLVEVNCETDFVARNENFTTIVKDVAMHIAA
                     SSPEFVSPDNIPEQAKAEKRAEFTEKVKAEGKPDKMVPQIVDGMLNKYFAERCLLEQP
                     FIKNPDITVDQYVKEGIAKLGENIVVRRFSRMALGEVV"
     gene            complement(2724..>3266)
                     /gene="rpsB"
                     /locus_tag="PKV52_02320"
     CDS             complement(2724..>3266)
                     /gene="rpsB"
                     /locus_tag="PKV52_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPD99080.1"
                     /translation="FAEKQTAAGHQILFVGTKRQAKDIVKTAAVYAGQPYVVERWLGG
                     MLTNSTTTNARIKKLKDLEGKMESGELASRYNKLEVQRFQEQIDAMNHNYGGIKDMNG
                     RPGAVFVTDILEDQNAVNEAIKLNIPVIAIVDTNTDPSKIEYPIPANDDAVGTLQLIV
                     NYIADAAQEGRGKKVAKAAE"
BASE COUNT          759 a          758 c          667 g         1082 t
ORIGIN      
        1 aatgggtgcc gaccacgtag attgtataag ggtatattcc cttgggacaa cccccaaata
       61 cccctttggt gtatcggtat ttttactatc tgctacttgt tgttcactaa gcagcgttgt
      121 agtcgccgtg gtagtttgac catcacgttc ataaacaata ggcaccgtgt tccctgcatt
      181 tgcagatgct acttcgcgta ccgatgcttc gttggtaatt ggttggttgt taagagatac
      241 gatggtgtcg cctacctgca aacctgcttg cgctgcaggt gagtctgcgt ctacatagcc
      301 agcaaatata tcgttacgga gcaccgtgct atctgatggc acactaaatt gattagggat
      361 tacttgtggc atccccaccc acgcaacaat agtaaagaac accagcgctg caagtaaatt
      421 gaccgctaca cctgccaaca taatacgcaa tttagccgat aaacccaccg agccaaacga
      481 acctttttct gtagcagcgt cttcttcgcc ttttagccgt acaaaaccac caagtggtaa
      541 caagttaatt gtgtaatagc cttcaaaaat gcccttgccc aactttttgc caaatagttt
      601 tggtggaaag cctataccaa attcttctac atctaccccg ttacgttttg ctaccaaaaa
      661 gtggccgtat tcgtgcagca ctataagtag cacaaacaaa aataagccta aaacaattaa
      721 ccacattacg cgccaaacct cctatggcgt gaagcatacg cagctaaggc ttcgtctaaa
      781 tcttctggtg taaaatctgg ccagagcttg tcggtaaaaa acagctcgct ataagctgcc
      841 cgccataaca taaaattact aatgcgcatt tcgcctgatg gccgtataag taaatctaca
      901 gggggcacag cacttccgcc atacagtgca gtttctatgc tctcttgcgt aattggcgtt
      961 cccatagctg cgagcttggc agctgcatcg gctatttctt gttggccacc atagttaaaa
     1021 cacaaacaaa atgttgctgt agtattattt ttagatgatt cttcggcgcg gcgtaattgc
     1081 ttaataattt tggcgtctag cccttcttcg ctacctagcc atataaagcg tatatctttt
     1141 ttggtaagtt cgcttatata tttgttcata acaatctgta taaagcgcat taaaaaagat
     1201 acttcgtctt tggcgcggtt ccagttttct gtagaaaaag caaaaacact taagtaaggt
     1261 atgccccgtg caagaccagc atcggcaatt cgccgaaacg tttcgaaccc ttgcttgtgg
     1321 ccttcttggg cagacacacc gtgctgcttg gcccaacggc ggttgccgtc aaggactata
     1381 cccacatgct gtggttgtgt atcgttatga accatatgtt atttcatcct cacatcttga
     1441 tagttttgtt ttagccatta gattgcgtga ttataccgtc attatctctt gttctttggc
     1501 tttggtggct gtttctatgt cttgtttagt tttggtcatt aaatcttcta cttgcttttc
     1561 tattctggtg taatcgtctt cactaatatc tttatctgct ttagcttgct tggcagagtc
     1621 taatgcttcg tggcgaatat ttctacatgc aatatgcgtt tcttctgtct tttcgttaag
     1681 ctgttttaca atttgctgcc gacgttctgt ggtgagtggt ggaatagaaa ttcgcacaac
     1741 ccgaccatca tctgctgggt taagccctag tgactgatca ttgcgtatcg catcagaaat
     1801 cgcctggatg ttactggggt caaacggagt gatttgtagc tgttgcgctt ctggtgcagt
     1861 tacgttgctg acttggttta acggcatggg ggtaccatat gctagtaccg taatgccatc
     1921 gagcatgctc gggtgcgccc gaccagtccg taattttttg atttcgtcat taaaatggtt
     1981 tacggccgta gctagtttgg tttttgcatc ttctactaat tggtttggac tcatgattcc
     2041 cccattacta tattgttagt atataccaaa cagcgttagt gcagcagtgg tattatttgt
     2101 taagcgagct ttttttaaac aacttcgcca agtgccattc ggctaaaccg acgcactact
     2161 atgttttcac ctagttttgc gatgccctct ttaacgtact ggtctactgt aatgtctggg
     2221 tttttaataa acggttgttc taataggcag cgttctgcaa aatatttatt cagcataccg
     2281 tctacaattt gtggcaccat tttatctggc ttgccttctg cttttacctt ttctgtaaat
     2341 tcggctcgtt tttctgcttt ggcttgttct ggtatgttat ctgggcttac aaattctggt
     2401 gagctagctg caatgtgcat tgccacgtct tttacaatag tcgtaaagtt ttcgttccgt
     2461 gccacaaagt ctgtttcgca gtttacttct acaagtacgc ctattcggcc atcgtgtagg
     2521 taacttccta taaccccagc gcgggcttcg cgttcaccac gtttttctgc tttggtcatg
     2581 ccttttttac gcatagcagc caaggcttta tcaaaatcgc cctctgcttc taccagagct
     2641 tttttggcat cggtaatacc aacacctgtt agttcgcgta gtcgcttaat tgtatctata
     2701 tcaattgcca tacttacccc cttttattca gctgcctttg ctactttttt accacgacct
     2761 tcttgtgctg catctgcaat gtaattcaca atcaactgca acgtacctac tgcatcatca
     2821 ttagcaggaa taggatactc tattttgctt gggtctgtgt tggtatctac aattgcaatg
     2881 accggaatgt ttagtttaat agcttcattc acggcgtttt gatcttctag gatatctgtt
     2941 acaaaaacag cccccgggcg accgttcatg tcttttatgc caccatagtt atggttcatg
     3001 gcatctattt gctcttggaa ccgttgaact tctagcttgt tatacctgct ggcaagctcg
     3061 ccactttcca ttttgccttc taggtctttg agctttttga tgcgtgcgtt ggttgttgtt
     3121 gaattggtaa gcataccgcc caaccatcgt tcaacaacgt atggctggcc agcgtatacg
     3181 gctgctgtct ttacaatgtc tttggcttgg cgttttgtac caacaaacaa tatttggtgg
     3241 cctgcagcag tttgcttttc tgcaaa
//
LOCUS       DAOPLS010000076         3234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_33430_length_3234_cov_3.751180, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000076 DAOPLS010000000
VERSION     DAOPLS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33430_length_3234_cov_3.751180"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKV52_02325"
     CDS             complement(<1..749)
                     /locus_tag="PKV52_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPD99081.1"
                     /translation="MNPRLARHTPNQLPVARKHRPDYGLFITASILLAVGLIVMYAIS
                     PALAAQGGDVSDTYFVVRQLIAVVIGIGAFFIFSKISVENWSKWHKALIISAIVLSLG
                     TIVTGGLANRWIQLGAFSFQTVEYVKLVVIIVFAGYLSQVIDKGKLTDIHAWRVPMII
                     ASVLALIVVIIQRDLGSAAVLFTITLAMLYMAGFPLAKLTIAITIIAILGVLAVVSTP
                     YRRDRVLTFLQPTADCSDAGYHSCQALIAVGS"
     gene            complement(746..1786)
                     /gene="mraY"
                     /locus_tag="PKV52_02330"
     CDS             complement(746..1786)
                     /gene="mraY"
                     /locus_tag="PKV52_02330"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPD99082.1"
                     /translation="MSTLNIAIETATLTEIMYLGLLGFIVSMALTPIYTTVAYKYKWW
                     KKPRDTALTGEKATEFQKLHAAKHRRNIPTMAGIVTLVGVALVTLVFNLSREQTWLPL
                     ALMAAAGVVGLIDDYINIRGQGKGVAGLRAKLKFSMIGTVAFVGGWWFFAKNDITSFY
                     MPGLGDVTIGWLMIPLFMLVVIATANAVNISDGLDGLAGGLLASSFGAYAVIAFLQER
                     YGVAAFCMTMVGSLLAYIWFNIYPARFFMGDVGSFSLGTALGVVAMITNTLVLLPIIG
                     FVFVAEAGSVIIQTISKKTRNGKKIFRISPVHHHFEAIGWPEAKVTMRFWVIGQVAAV
                     VGLVLAIVGGFV"
     gene            complement(1826..>3234)
                     /locus_tag="PKV52_02335"
     CDS             complement(1826..>3234)
                     /locus_tag="PKV52_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPD99083.1"
                     /translation="PIQCSVGIIIIVAKKIDEAQSKRVEDLKIKGVGLKEISVRSYPQ
                     GTVAAHVLGFVNDDGVGQYGFEEYFNKELTGKSGQQKIITDVNGVPLAINNESVQTQP
                     DPGSDVTLSLDIRLQRIVEEQLKAGVDRTQTKRGSAVLIDPNNGEIKAMANYPTFDPS
                     QFASQADLSVFTNTAVSRAWEPGSVMKPLMLATALQEGAVTRNTSYFDPGYKKIADTT
                     VTNAHSYGARTMTIDDIITKSLNTGAVHLMQSLGGGDITEASRQTWYEYLTNHFMFNK
                     TTGIELANEQPGGVIPPQDNGAGINVTYANMAFGQGLTITPIQLAAAYAALVNGGTYY
                     QPTVIEEYKKDGTSVKQEPKVVRSDVVSSEVSAQVKDILRESLEENYKPAIRAGYTLG
                     AKSGTAELSDGQGNYREDAYDGAYVGYIEGKKLEYVMVVRLDEPKTSGFASRQATIVW
                     GDISNEIINSIAIEPKIQ"
BASE COUNT          919 a          794 c          606 g          915 t
ORIGIN      
        1 gacccaaccg caatcaaagc ctggcatgaa tgataaccag catcagaaca atcagccgtt
       61 ggttgtaaaa aagtgagtac acggtcacgc ctatatggtg tagaaacgac tgctaatacg
      121 ccgagtattg caatgatagt aattgcgata gttaacttcg caagtgggaa cccagccata
      181 tatagcattg cgagagtgat cgtaaagagc acagccgcag aaccaagatc cctctgtata
      241 atcacgacga tgagcgctag tacactcgcg ataatcatcg gcacacgcca cgcatgaata
      301 tcagtcaatt tacctttatc tatgacttgc gataaatagc cagcaaagac aataataacc
      361 acgagcttta catattcaac tgtttggaac gaaaaagccc caagttgtat ccatcggtta
      421 gccaaccccc ctgtcactat tgttcctaag ctcaaaacaa ttgcactaat aattaaggct
      481 ttatgccatt ttgaccaatt ttctacagaa atttttgaaa aaataaaaaa agcacctata
      541 ccaattacta cagcaataag ctgtcgaacg acaaaatatg tatcactaac gtcaccgcct
      601 tgcgcggcga gtgctggact tattgcatac attactatta ggccaacagc tagtagtata
      661 ctggcagtaa taaatagccc ataatcaggt cgatgtttac gggctactgg taactgattt
      721 ggtgtatgcc gcgcaaggcg cgggttcata caaaacctcc tactatagca agtactaggc
      781 caacaacagc ggctacttgc ccgattaccc aaaaacgcat tgtcactttc gcttctggcc
      841 aaccaattgc ttcaaaatga tggtgtacag gactaatcct aaatattttc ttaccattac
      901 gagttttttt agatatcgtc tgtataatta ccgaaccagc ctcggctaca aatacaaacc
      961 caatgattgg taataacacg agcgtgttcg ttatcatggc aacaacaccc agtgctgtac
     1021 cgagactaaa actcccaaca tcacccataa aaaagcgtgc tgggtagata ttaaaccata
     1081 tataagcaag cagcgatccg accatcgtca tgcaaaaagc agcgacaccg taacgttctt
     1141 gtaaaaatgc tatcacagcg taggcaccaa aagatgatgc caataagcca cccgccaatc
     1201 catcgagacc atcagaaata ttcacagcat tcgcagtcgc gataacaacg agcataaata
     1261 gcggaatcat cagccagccg attgttacat cacctaaccc cggcatataa aatgacgtaa
     1321 tatcattctt tgcaaagaac caccagccac caacaaacgc aaccgtacct atcatactaa
     1381 actttaattt tgcgcgtagc ccagcgaccc ccttgccttg ccctctgata ttaatgtaat
     1441 catctatcaa acccaccaca ccggctgcgg ccattaaagc aagcggcaac caagtctgtt
     1501 cgcgggataa attaaatacg agggtaacaa gcgcaacacc tacaagcgta acaataccag
     1561 ccattgtcgg aatattacgg cgatgtttgg cggcgtgcag cttttgaaat tcggtcgcct
     1621 tttctccggt tagtgctgtg tcgcgtggtt tcttccacca cttgtactta taggcaactg
     1681 ttgtgtatat cggtgtaagg gccatactta caataaagcc aagcaagcct aaatacatga
     1741 tttctgttaa ggtagcggtt tctattgcta tatttaatgt tgacatcttt gtatcttctt
     1801 ctttgtagtt attagtatac cataattatt gaatctttgg ttctatagcg atagaattaa
     1861 tgatttcgtt agaaatatct ccccaaacaa tggtcgcttg cctactggca aagccactgg
     1921 tctttggctc atctagccgc accaccataa catattctag ttttttgcct tctatgtacc
     1981 caacgtatgc tccatcgtag gcatcttcac ggtagtttcc ttggccatca gacagctcgg
     2041 ctgtaccact ctttgcacca agcgtatagc ctgcccggat ggctggcttg taattctctt
     2101 ctaatgattc acgtaaaata tcttttactt gcgcactcac ttcgctgctc actacatcac
     2161 ttcttacaac ttttggttct tgcttcacgg atgtgccatc ttttttatat tcttctatga
     2221 ccgttggctg ataatatgtt ccaccgttaa cgagcgcagc ataggctgcc gccagttgta
     2281 ttggtgtgat tgttaagcct tgaccaaaag ccatattagc ataggtgaca ttaatacccg
     2341 ccccattatc ttgaggggga ataacaccgc ctggttgttc attagcaagt tcaatgcctg
     2401 tcgttttatt aaacataaaa tgattcgtta aatattcata ccatgtctgc cggctcgctt
     2461 cagtgatatc accaccccca agcgactgca ttaagtgcac cgcaccagta ttgagtgact
     2521 ttgttatgat gtcatcgata gtcatcgtcc gggctccata actgtgggcg ttggttactg
     2581 ttgtatctgc aattttctta taacctggat caaagtatga agtattgcgt gtgacggctc
     2641 cttcttgcaa agcagtcgca agcataagcg gtttcatcac cgaacctggc tcccaggccc
     2701 tgctcactgc tgtattggta aacacactta aatctgcttg gcttgcaaat tgcgacggat
     2761 caaacgtagg atagtttgcc attgccttaa tttcgccgtt gttagggtct attagtacag
     2821 cgctccctct tttagtctga gttctatcaa cgccagcttt caattgctct tcgacaatac
     2881 gctgcaagcg tatatctaag cttaaagtca catcactccc cgggtctggc tgcgtttgga
     2941 cggattcgtt atttatggca agtggtaccc cattcacatc tgtaataatt ttctgctggc
     3001 cacttttgcc tgtcagctct ttgttaaaat actcttcaaa accatattgg ccaacgccgt
     3061 catcattaac aaacccaaga acgtgtgccg ccacagttcc ctgaggatac gaccgtacag
     3121 aaatttcttt aagaccaaca cctttgattt ttaaatcttc tactcttttt gattgtgctt
     3181 cgtctatttt tttggcaaca attattataa tgccaacact acactggata ggaa
//
LOCUS       DAOPLS010000077         3184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_34131_length_3184_cov_4.808565, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000077 DAOPLS010000000
VERSION     DAOPLS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34131_length_3184_cov_4.808565"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /gene="argS"
                     /locus_tag="PKV52_02340"
     CDS             <1..1297
                     /gene="argS"
                     /locus_tag="PKV52_02340"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPD99084.1"
                     /translation="LVGNAVANILEAVGGRVYRLNYGGDVGLHAAKAMWGIIDTLGGE
                     FPNKLQDIPESDRPSWVSQCYVAGNTAYESSEQHKQAIAEINQKVYAIHEQNDHESDF
                     AQIYWITRNWSYDGFKKMYKELQVHAFDEFIPESTVTPLGKQIVEEGLAKNVFETSDG
                     AIIYSEKKSGLHTRVFITSKGLPTYEAKELGLAVYKWQKYQFDKSLVITANDITEYMK
                     VLFSAIAHFYPEVEQRTTHLTHGMIKLSGGRKMSSRTGNVLMAADIINSATQANKESS
                     GKDDEAVAIGAIKYAFLKQRIGGDISYDPEESISLNGNSGPYLQYAHARARSILAKIP
                     TSSDSKVVVDTLDTHERALAVKLSDYAETINKAANDLMPHHICSYLYELAQVFNRFYE
                     NSKVVGDPRQAIRRTLVQQYANTLKTGLEILGITAPNKM"
     gene            1887..>3184
                     /locus_tag="PKV52_02345"
     CDS             1887..>3184
                     /locus_tag="PKV52_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99085.1"
                     /translation="MAETMYKGEPINGDFAGKDFISIQQVSRSDLEIVYSAADVLRVI
                     REEKLPISLLNNQYQLANVYYEPSTRTSSSHAAAWRRLGGGVDTINNVSFSSVVKGES
                     LKHTVQTLEAFSEMVVLRHGQVGAAALAALALDKPVMNAGDGVGEHPTQALLDGYTLE
                     RELGGIDDKVITIAGDLLHGRTTHSLMQLLALYKPRRVNLVAPPEYQMPQQYIDLVTS
                     KGIEVVLHVGLEAVLPETDALYMVRLQRERLESDTHLPVEKQVVKKVNKIINHGSALQ
                     KEALGTDFDAALERLFGTIKHSSSESERDMARFLLYAINGTLPEVKVLKNGNGFNDNI
                     YVLTPELMATMKDEAKVLHPMPIAGEIEDEVDADPRAVYLEQVENGMYVRGALMSEIM
                     DVNVHEAKLQYDMDQERVAAIRRYADYLKESDHVPPEINW"
BASE COUNT          980 a          633 c          747 g          824 t
ORIGIN      
        1 gctcgtgggt aacgcggtag ctaacatact agaagccgta ggtgggcgcg tgtatcggct
       61 taattatggt ggtgacgtgg ggttgcatgc tgccaaggca atgtggggta ttattgatac
      121 gcttggtggt gagttcccca ataagctaca agatattcca gaatccgatc gcccttcgtg
      181 ggtaagccaa tgttacgttg ctggtaatac ggcatatgaa tcgagcgaac aacacaagca
      241 agcaattgct gaaatcaacc aaaaagtgta tgcaatccac gaacaaaacg atcatgaatc
      301 tgattttgca caaatatact ggataaccag aaattggagc tatgatggct ttaagaaaat
      361 gtacaaagaa ttgcaggttc atgcgtttga tgaatttata ccagaaagta ccgtgacacc
      421 acttggtaaa caaattgtag aagaaggcct tgcaaaaaat gtgttcgaaa catcagatgg
      481 tgcaattatt tacagcgaaa aaaaatctgg ccttcataca cgggtattta ttacctctaa
      541 aggcctgcca acgtacgaag caaaagagct aggtttagca gtctataagt ggcaaaaata
      601 tcagtttgat aaaagccttg tcattacagc caacgacatt acagaatata tgaaagtgtt
      661 gtttagcgct atcgcgcact tttacccaga agtagaacaa cgtacaacgc accttacgca
      721 tggcatgata aaactcagtg gcgggcgtaa aatgagtagt cgcacgggta atgtgcttat
      781 ggccgcagat attataaatt ctgccacgca agcaaataaa gaaagttctg gcaaagatga
      841 cgaagctgta gcaattggcg caattaaata tgcattttta aaacagcgaa taggcggtga
      901 tatatcctac gatccagaag agtctattag tcttaatggc aacagtgggc cgtatttgca
      961 atacgctcat gcaagagcgc ggagtatatt ggccaaaata ccaaccagca gcgattcaaa
     1021 agtagtcgtt gacactttag acactcatga gcgtgcccta gcggttaaac tatcagatta
     1081 cgcagagact atcaacaaag cagccaacga tctcatgccg caccatatat gtagctattt
     1141 atacgaactt gcccaagtgt ttaaccgttt ttacgaaaac agcaaagtgg ttggcgatcc
     1201 gcgccaagca attaggcgaa cattagttca gcaatatgcc aacacgctaa aaactggcct
     1261 tgaaatccta ggcataaccg cccccaataa aatgtagtat ttacaaaaca ataataaagt
     1321 gctataatat atatgtctat tatagtggcc ggatagtccg gaacaaccac tactaccacg
     1381 acgagaggtg gtaaaaatgg actatgcagc aatcgtagca atagttggtc ctatcttggg
     1441 gctgacttcg tgctatcttg gtatctacag catgggggac gacaagttcc cgctgctggt
     1501 gagcctagtc atgttctgtt tgggcatgac aacgtgtgtg gtgtccgttc ttgcgacagc
     1561 aggaacgtac cccctgttgg tgagtgggag ccttgctctt gccttgatcc cgggcatatt
     1621 gttctgggtg agcaccattt tcgatgcgtg gcgtgagtag cgttgcaaaa aggctttcta
     1681 ggtgggccga ttcaaaccac ctaaatcgtc ccgcgtgtgg ctacaaaccc cgcgggactt
     1741 tctcttggct ctgaattgac acgtacctaa actatatgat tacctatagt gtagtatgaa
     1801 aatgttttgc aaaactaaat ttacgcaaat atagagtcgc caatggcggc tcttttttta
     1861 tatgtataac aagaaaggat taagtagtgg cagaaacaat gtataaaggc gaaccaatta
     1921 acggagattt tgctggtaaa gattttatat ctattcaaca agttagcaga agcgacctag
     1981 aaatagtata ttctgcagct gatgtgttgc gcgtaatacg tgaagaaaaa ttgcctatct
     2041 cattacttaa caatcaatac caacttgcta acgtgtatta cgagccttca actaggacat
     2101 catcatcaca tgccgctgca tggcgtaggt tgggtggtgg cgtagacact ataaataatg
     2161 taagtttttc ttctgtcgtt aaaggagaat cattaaaaca cactgttcaa accctagagg
     2221 ctttttcaga aatggtggtg ctgcgtcatg gtcaagttgg cgcagccgca ttggcggcac
     2281 tggcgctaga caagcctgtc atgaacgcag gcgatggcgt tggtgagcat cctacacaag
     2341 ccctgcttga tggctatact ttagaacgcg aacttggtgg catagatgac aaggtaatta
     2401 caattgcagg tgacttatta catggccgta ccacgcattc actcatgcag ctcttggccc
     2461 tgtataagcc acgccgggtt aatttagttg cgcctccaga atatcaaatg ccccaacagt
     2521 acattgattt agtaacttcc aagggtatag aagttgtgct tcacgttggc ctagaagcgg
     2581 ttttaccaga aacagatgct ttgtatatgg tgcgtttgca gcgtgagcgg ctagaaagtg
     2641 acactcattt gccggtagaa aaacaagtag taaaaaaagt taataaaatc atcaatcatg
     2701 gtagtgcgct gcagaaagaa gcattgggta cagattttga tgctgcactg gagcggttat
     2761 ttggaactat caagcattca tctagtgaat cagagcgaga catggcacgg tttttgctgt
     2821 atgcaataaa tggcacattg ccagaagtaa aagtactaaa gaatggcaat gggtttaatg
     2881 acaatatata tgtgctaacg cctgaactta tggctactat gaaagatgaa gcaaaagtct
     2941 tacaccctat gccaattgca ggtgaaattg aagacgaagt agatgccgat ccaagagcgg
     3001 tgtacctaga acaagtagaa aatggtatgt acgttcgtgg tgcattgatg tctgaaataa
     3061 tggacgtcaa tgttcatgaa gcaaagctac aatatgatat ggatcaagag cgcgtggcgg
     3121 ctattcgccg gtatgcagac taccttaaag agagtgatca cgttcccccc gaaattaatt
     3181 ggca
//
LOCUS       DAOPLS010000078         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_35829_length_3078_cov_3.679788, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000078 DAOPLS010000000
VERSION     DAOPLS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35829_length_3078_cov_3.679788"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PKV52_02350"
     CDS             complement(<1..838)
                     /locus_tag="PKV52_02350"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPD99086.1"
                     /translation="MVTKVAINGFGRIGRNAFKVAFERKDIEIVAINDLTDTKTLAHL
                     LKHDSNYGTYHHEVASDEEHIIVDGKPVRVYKQTDPALLPWGDLEVDVVIESTGFFVD
                     PAKARAHIDQAGAKRVVISAPAKGEGATTIVLGVNEDKLEGASDIISNASCTTNCITP
                     VMDVISQNFGIDKAMMTTVHSYTASQRLQDAPAKDLREARNAAENIVPTTTGASIAAG
                     QALPEIAGVFGGLSVRVPTPVVSLSDFVIVTKQTVTVEAVNDAFKKAASEPYYQGILA
                     ITN"
     gene            complement(904..1431)
                     /locus_tag="PKV52_02355"
     CDS             complement(904..1431)
                     /locus_tag="PKV52_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010198287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPD99087.1"
                     /translation="MADFNKVLSPGDWQKALVNTVIEIPKGSSHKIEWDREHAAFMLD
                     RVEPAIFAKPVNYGFIPGTLDEDGDELDTLVVTNEPLPTGIWLEAKVIGILNFEDDGE
                     MDYKIVCVPADDRQTGDSINSLEDLGTRWQQQIEHHFTHYKDLKKPGSTKVLGWGDVA
                     EAKTIIAECVERYSN"
     gene            1476..2123
                     /locus_tag="PKV52_02360"
     CDS             1476..2123
                     /locus_tag="PKV52_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPD99088.1"
                     /translation="MQIISHRCETLSAIHAAATYDVAYIEFDIRYTKNNKIVVYHNDK
                     TPSGKTIAHITLKALQREVEDTCELAPALVACGNRPALVESKCKGTIARSLPELKKNH
                     RAAVASFHADEILAARINLPSHKTFLLQRYHPFGIIRKALNVDAHGIGLNKNWILLFP
                     YYYNQAQKNELAIYTYTVNNLWLGRRLQALFKKLFICTDYPHKFTKTTPASSTKS"
     gene            2120..2506
                     /locus_tag="PKV52_02365"
     CDS             2120..2506
                     /locus_tag="PKV52_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HPD99089.1"
                     /translation="MTKRWRIIVVITILGLIIGSTTAYLLMKKSESRQDIPLPSLQKE
                     ITLADMQNHANETSCWTYIGGQVFDATTVIRDFPDYKTILLRTCGTDGSDVFVVKKYE
                     TQQLTKETLTQLRQTLNNHQIGLLIP"
     gene            complement(2529..>3078)
                     /locus_tag="PKV52_02370"
     CDS             complement(2529..>3078)
                     /locus_tag="PKV52_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPD99090.1"
                     /translation="LGIAIAGVSIWFIVYGGKDETTANDTATTNSQQSDATTQEQKDS
                     LDSILKKQKQIKIFNELLVEADVVTTLQGQGPYTVLAPTDDAFKALPEGLLDTLKKPE
                     NKDNLRKLMQYHIVKGNLSAESLQNGQKLPTVNGQEIIIEISDGKTSFVDAKGNKALI
                     KTADVAASNGTIHILTAVLLPQ"
BASE COUNT          811 a          686 c          587 g          994 t
ORIGIN      
        1 cattggttat cgctaaaatg ccttggtagt atggttcgct agcggctttt ttaaaagcat
       61 cgtttactgc ttctacagtt accgtttgct tggtgacaat aacaaagtca gacaagctga
      121 cgacaggtgt cggtacgcgc acacttaacc caccaaatac tccagctatt tctggtaatg
      181 cttggccagc tgcaattgat gctccagttg tagtaggtac aatgttttcg gcagcattgc
      241 gggcttcacg caaatctttg gccggtgcat cttgcaaacg ttggcttgca gtataactat
      301 gtactgttgt catcattgct ttatctatac caaagttttg gcttattacg tccattactg
      361 gtgtaatgca atttgtcgta cagcttgcat tacttatgat gtcacttgca ccttcaagct
      421 tatcttcgtt tacccctaag acaatagtgg ttgcgccttc accttttgcc ggtgcactaa
      481 tcactactcg ctttgcccca gcctgatcta tgtgtgcgcg ggctttcgca gggtcaacaa
      541 aaaagcctgt actttctatc acgacatcta cctcgagatc accccaaggt aaaagagcgg
      601 ggtcagtttg cttgtacaca cggaccggtt tgccatcaac aatgatatgt tcttcatcgg
      661 acgctacttc gtgatggtat gtaccatagt tactatcatg tttaagtaga tgggcaagcg
      721 tttttgtatc tgttaaatca ttaattgcta cgatttcaat atcttttctt tcaaatgcta
      781 ccttaaatgc attgcgccct atacgcccaa agccattaat tgctaccttt gttaccatgt
      841 gtccctccct gattgttgta cgtttatgtt tattgtatat gcttatggca ttcgcctcaa
      901 gcattagtta ctatatcgct ctacacactc tgcaattatt gttttggctt ctgccacgtc
      961 gccccagcca agcactttgg tactgccagg tttttttaaa tctttatagt gggtaaagtg
     1021 gtgttcaatt tgttgttgcc accgggtgcc taaatcttct aggctgttaa tgctatcccc
     1081 tgtctgacga tcatctgcag ggacgcatac gattttgtag tccatttcac catcgtcttc
     1141 aaaatttaat atgccaatca cttttgcttc tagccatatg cccgtgggca gtggttcgtt
     1201 cgtaacaacg agggtatcta attcatcgcc gtcttcatcg agcgtaccag gaataaagcc
     1261 atagtttact ggtttggcaa aaattgctgg ctctacacgg tctaacataa acgcggcatg
     1321 ttcacgatcc cactcaattt tgtgggaact ccctttcggt atttcaatta cagtgtttac
     1381 gagcgctttt tgccaatcgc ctggacttag tactttatta aaatcagcca tgtattctcc
     1441 taaattatct acctatgtag tatacaataa ttattatgca aattatttct catcgttgcg
     1501 aaacactcag cgctatccat gctgctgcta cgtacgatgt agcgtatata gagtttgata
     1561 tccggtacac gaaaaataat aaaatagttg tgtatcataa cgataaaaca ccatctggca
     1621 aaactattgc acatataacc ttaaaagcat tgcagcgtga agttgaagat acctgcgagc
     1681 ttgctccggc cttggttgcc tgcggtaaca ggcccgcttt ggtagaatca aagtgtaaag
     1741 ggacgattgc tcgttctctg cccgagctta aaaaaaacca tcgggcagca gtagccagct
     1801 ttcatgcaga tgaaatttta gctgcgcgta tcaacttgcc gtcacataaa acatttttgc
     1861 tgcaacgata ccatcctttt ggaattattc gcaaagcatt gaatgtagat gcgcacggta
     1921 tagggctgaa caaaaactgg atcttgctgt tcccttacta ttacaaccaa gcgcaaaaaa
     1981 acgaactagc tatctatacc tacactgtta ataacctatg gctagggcgt cgtctccagg
     2041 cgttattcaa aaaattgttt atttgtactg actatcctca taagtttaca aaaaccaccc
     2101 ctgcgagcag cactaaatca tgactaaacg ttggcgcatt attgttgtaa ttactatatt
     2161 agggcttatt attggcagta ccaccgctta cttattaatg aaaaaatcag aatctcgcca
     2221 agatataccg ttgccttcgt tgcaaaaaga aatcactttg gctgatatgc aaaaccatgc
     2281 aaacgaaaca agttgctgga cgtatatcgg aggtcaagta tttgacgcta caactgttat
     2341 acgggatttt ccagactata aaacaattct tttacgcact tgcggtacag atggttcaga
     2401 tgtgtttgtc gtaaaaaaat acgaaacaca acaactaact aaagaaacac tcacacaact
     2461 acgtcagaca ctgaacaatc accaaattgg tttactaatc ccttaatacc tacggtttta
     2521 ttgcttagtt attgtggcaa taagaccgct gttaaaatat gaatggtacc attagacgcc
     2581 gcgacatctg ccgtttttat aagggccttg ttacctttcg catctacaaa gctcgttttg
     2641 ccatcactta tttctatgat gatttcttga ccattgacgg ttggaagctt ttgaccgttt
     2701 tgcaaactct ctgccgataa attacctttg acgatatggt attgcattaa tttacgtaga
     2761 ttgtctttat tttctggttt cttaagtgta tctagtaaac cctctggcag agctttaaag
     2821 gcatcgtcgg ttggcgcgag tactgtatac ggaccctgcc cctgtaacgt agttaccaca
     2881 tcggcttcta cgagtagttc attaaatatt ttaatctgtt tttgcttttt taatatgctg
     2941 tctaggctat ctttttgctc ttgcgtggtt gcatcgcttt gctgtgaatt ggttgtagct
     3001 gtatcatttg ctgttgtttc atctttacca ccatatacaa tgaaccatat acttacacca
     3061 gctatggcga tccctagt
//
LOCUS       DAOPLS010000079         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_37563_length_2977_cov_4.404517, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000079 DAOPLS010000000
VERSION     DAOPLS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37563_length_2977_cov_4.404517"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKV52_02375"
     CDS             complement(<1..684)
                     /locus_tag="PKV52_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HPD99091.1"
                     /translation="MNGTELQTVLTDLQTQVSADMQRLDIASAEALISNITTAQQSPD
                     FWQDAQAAQASMQQLSKLNQKVQPWQALAGTIADTLELLALQDDALLPEITKTVEILQ
                     VEYAACKKLAQYNGPYDDHDVIITLYSGAGGTDAQDWAQMLLRMYTRWAEQNKYTVSL
                     LQESPGEEAGIKSATLQIGGALAYGKLKGEHGVHRLVRLSPFNADNLRQTSFAKVDVL
                     PKIDAPEEVA"
     gene            complement(694..1974)
                     /locus_tag="PKV52_02380"
     CDS             complement(694..1974)
                     /locus_tag="PKV52_02380"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPD99092.1"
                     /translation="MQHNVRHVTLKNGAEGLYIDMPGAGVVAIDIVFRAGDYLSPAGK
                     TDTAHVMEHLVLGANKKYPSSKEYSREFSKYGAYNNAYTGDYHMGYEAECEASEAARI
                     TDLLCDAIEAPLFTQTDFIAEIGNVREELKMRRNNDDVELSLMLEDAMGFVPKSFKQR
                     ESELSAITLADVHAHYKKTHTSSNMRFVIAGPMDHLQKPITERLASIALPKGTGYIEL
                     PDEQPKQLKSPLLVRNANLDNLCYRWEAVLPMFSSLVQRDSLSALQELLFNGIHSRVF
                     GDLREKGLVYGIFGSNYETKHNVVNVISGQVQTANIADVFKILHKEIATIANNGLPEK
                     EVQDLRKRAYGEVQRYNQTASQLSGWYRQPFITRNEIIYFDAFAERLEHVTSNSIQAI
                     AQAILDAPINGLGIMHNDTEVPDARQLFAKLKQG"
     gene            complement(1974..>2977)
                     /locus_tag="PKV52_02385"
     CDS             complement(1974..>2977)
                     /locus_tag="PKV52_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPD99093.1"
                     /translation="GSERHESRRIDNQLRGRTGRQGDPGATQFYVSTEDDLMRVFGGD
                     KIRSLMERLKVDEDMPLENRVVSKSLENAQKKVEGFNFDIRKNVVKYDDVMNRHRKVT
                     YETRRALLENANIADRIAKFIHEESVNLASLPGQDDEYEKLITDVFPFDDETLDRLFD
                     APADKFAQVLEQEATELYNARVTAFTPDVMRVVERDIYFQTLDNMWMQHLETMEHLRQ
                     GIGWTSVGQRDPLVEYRKRAQAIFETMQNSLRREVIRNVMHAMPLGYSAEHAQMHETE
                     LTRAARQSVDNADSITEAEIITQADFKPIKAQPKGTSTKKKRAARKVQRKNRKKSRR"
BASE COUNT          684 a          691 c          651 g          951 t
ORIGIN      
        1 agcaacttct tctggtgcat ctatttttgg cagtacgtct accttggcaa aactcgtttg
       61 gcgcaaatta tcggcattaa aagggcttaa gcgcaccaaa cgatgtacac catgttcgcc
      121 ttttagcttt ccgtacgcca atgctccacc tatttgtaag gtagcacttt ttattccagc
      181 ttcttcgccg ggcgattctt ggagtaagct aaccgtatac ttattttgtt ctgcccaacg
      241 ggtgtacata cgcagcagca tttgcgccca atcttgcgca tcggtgccac ccgcccctga
      301 atacaatgtg attataacgt cgtggtcatc gtacgggcca ttgtactgtg caagtttttt
      361 acatgctgcg tattctactt gaaggatctc cactgttttg gtaatttctg gtagcaacgc
      421 atcatcttgc aatgctaaaa gctctaacgt gtctgctatt gtgccagcaa gtgcctgcca
      481 cggctgtact ttttggttca gcttgctgag ttgctgcata ctggcttgcg ctgcttgtgc
      541 atcttgccaa aaatctggcg attgctgggc agtagtaatg tttgatatta aagcttcggc
      601 agacgctatg tctaatcgtt gcatgtcggc agaaacttgc gtttggaggt ctgttagtac
      661 ggtttgtagt tctgtgccgt tcatgtttag tttttaccct tgttttagtt tggcaaataa
      721 ctggcgtgca tcgggcactt ctgtatcgtt atgcataatg ccaagcccat taataggtgc
      781 atctaaaatg gcctgggcaa tggcttgaat actattactt gtaacatgct ctaagcgctc
      841 tgcaaacgca tcaaaataga taatttcgtt acgggtgata aacggctgcc tataccagcc
      901 acttagttgg ctggcagttt ggttgtagcg ctggacctcg ccgtaggcac gttttctaag
      961 gtcttgtact tctttttctg gcagaccatt gttggcaatt gtagcgattt ctttgtgcaa
     1021 aattttaaaa acgtctgcga tgttagctgt ttgcacttgc ccgctaatta catttacaac
     1081 gttatgtttt gtttcgtaat tactcccaaa aataccatag actaggccct tttcgcgtag
     1141 gtcgccaaac acgcgtgaat gaatgccgtt aaataatagt tcctgcaaag cactaaggct
     1201 atcacgctgt acaaggctac taaacatagg cagcactgct tcccatctgt agcaaagatt
     1261 atctaaatta gcattacgta caaggagtgg gctttttagc tgttttggct gttcgtcagg
     1321 aagctctata tagccggtgc cttttggcaa ggctattgat gctagccgct cagttatagg
     1381 tttttgtagg tgatccatgg gaccagcaat gacaaagcgc atattactag acgtgtgggt
     1441 ttttttatag tgagcatgca catctgccaa ggtaattgca gatagttcag attcacgctg
     1501 cttaaaactt ttgggtacaa accccattgc atcctcgagc atcaaactaa gttctacgtc
     1561 atcattatta cggcgcattt tgagttcttc gcggacattg ccaatttctg cgataaaatc
     1621 tgtctgcgta aataatggtg cttctattgc atcacagagc aaatcggtaa tacgagcggc
     1681 ttcgctggct tcgcattcgg cttcgtagcc catgtggtaa tcacccgtat aggcattgtt
     1741 atacgcaccg tatttactaa attctctgct atattctttg gaactcggat attttttgtt
     1801 cgcgccaagt actaaatgtt ccatcacgtg ggctgtatct gttttgccag ctggtgataa
     1861 ataatcacct gcccgaaaca caatatctat agccaccaca cctgcgcccg gcatatcaat
     1921 atacaagcct tctgcgccat ttttaagcgt aacgtgccga acgttatgct gcattaacgt
     1981 cggctctttt tgcgattttt acgttgtact ttacgagctg cacgtttttt ctttgtgctg
     2041 gtgccctttg gttgcgcttt tatgggttta aagtcggctt gtgtaatgat ttctgcctct
     2101 gtaatactgt cggcgttatc taccgattgg cgggcggctc gggtaagctc tgtttcatgc
     2161 atttgagcgt gttctgcact atagccgagt ggcatggcat gcatgacatt acggataacc
     2221 tcacgacgca aactattttg cattgtttca aaaattgctt gtgcgcgttt acggtattct
     2281 accaacggat cgcgttgccc aacactcgtc cagccaatac cttggcgcaa atgttccatc
     2341 gtttctagat gttgcatcca catgttgtct agcgtctgga aataaatatc acgctcgacc
     2401 acgcgcataa cgtctggtgt aaatgctgtc acccttgcgt tatacagttc ggtggcctct
     2461 tgctcgagca cttgggcgaa cttatcagct ggagcatcaa ataatctatc cagcgtttca
     2521 tcatcaaacg gaaatacatc agtaatgagt ttttcgtatt catcgtcttg gcctggcaaa
     2581 ctggccaaat tcactgattc ttcgtggata aatttggcta ttctgtctgc gatgttcgcg
     2641 ttttccaaca acgcacggcg cgtttcgtag gtgactttac gatgcctgtt catgacatcg
     2701 tcatacttaa ccacgttttt acgaatatca aagttaaagc cttctacttt cttttgtgca
     2761 ttttctaagc ttttactgac aaccctgttt tctagtggca tgtcttcgtc taccttgagc
     2821 cgctccataa gactgcggat tttatcacca ccaaagacgc gcataagatc gtcttctgtg
     2881 ctgacataaa attgtgtagc cccagggtca ccttggcgac cagtacggcc acgcagctgg
     2941 ttatctatgc ggcgtgattc gtggcgttca gaaccaa
//
LOCUS       DAOPLS010000080         2960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_37858_length_2960_cov_3.403787, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000080 DAOPLS010000000
VERSION     DAOPLS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37858_length_2960_cov_3.403787"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..872)
                     /locus_tag="PKV52_02390"
     CDS             complement(435..872)
                     /locus_tag="PKV52_02390"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HPD99094.1"
                     /translation="MLGPVDYIVVGFKGNNFDGSVLEELSIATQKGTIRVIDLLFIMK
                     DADGNVMGGELNDQPAELKEAFASFGIEAGHELITEEDIEKIGMAMENNTAAGVLVIE
                     QLWAKGLKAALIDAGGELLDEGRIHPEKIASALKELSEIVEEE"
     gene            complement(1008..1316)
                     /locus_tag="PKV52_02395"
     CDS             complement(1008..1316)
                     /locus_tag="PKV52_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99095.1"
                     /translation="MEPTSKKSSNGLSILLGVLLVIALLIILGLAYNQYQQNQKINDS
                     IDAVNKSINSLQESVKTTQQTITESQTQKTEDTTNTTPASPTTPSDTTTTTDNSSTTN
                     "
     gene            complement(1404..2363)
                     /locus_tag="PKV52_02400"
     CDS             complement(1404..2363)
                     /locus_tag="PKV52_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPD99096.1"
                     /translation="MSVLSLTKVRNISKRDLLLTGLMALATYALIQQITNIDFTEVWN
                     AIKSADLVWFMLAFFVAQLILVPNATSMMSAVTAPIPFKPTLILQSAIQFIGLAVPST
                     ASRVATNVAYLKKFKVPYVTAITQDALDSFTGFLVQMTILVIAIIFGDVNLNLKNNIN
                     TDWVIVLSIAGGIILIGLIVLYNAKTLRLKLINILKDVIGSFSILAEHPIRLVTLFGS
                     NLLSQLVLGTTMVITAQAFGVDISIATALVIVVSATLLGGIAPVPGGIGVQEAIISAG
                     LVSAGVDQSSAYAMTVSYRLFTFFLPPIWGAFSFRWLTKNKFI"
     gene            complement(2582..>2960)
                     /locus_tag="PKV52_02405"
     CDS             complement(2582..>2960)
                     /locus_tag="PKV52_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99097.1"
                     /translation="DEKYSRMLTLYRLPMALFGELVLLIEPILLVYIIYLSIAKAYPF
                     ALLGAYVTITLYVLWTVWPDEHMPNREKMRMSLYSPIMYFIFYVMNVVQLTSILRCIK
                     NNKKVLRKVDTGGSWISPERATI"
BASE COUNT          885 a          593 c          511 g          971 t
ORIGIN      
        1 attccgccac tcgcccatgt agatatacaa cagtatacca aaatggaggc acctacgggt
       61 aacgctcccg tctacgcagt tttgcagact gctgcctaac ttctcggcca aggcgcctta
      121 cttacaaaat tatacagtat acagggccac ttgtgcatga agttgtactt catgtagttc
      181 atcgcttcac aatctctacg atatttttaa ggtaaatgag tcattacatt atcttattgg
      241 atatcattaa gaaatataac cgtgctactt aattgtaaag tagcacccta cgataggtgc
      301 tacatactta gattgctatc ttttcacaga tcttttacaa gacaccacga cggcgacggc
      361 gcctattcac tcctgccaca gatactggtg ataaaggtcg tccaatcata atattgttcc
      421 tttcttagtt attactactc ttcttctaca atttcactta gttctttcag agcactagcg
      481 attttttcag gatgtattct accctcgtct aataattctc caccagcatc gataagcgct
      541 gcctttaagc ccttcgccca tagctgctca ataactaaga cacctgcagc tgtgttgttt
      601 tccattgcca taccaatttt ttctatatct tcttctgtta ttagttcatg accggcttct
      661 atgccaaaac tagcaaaggc ctcttttagc tctgctggtt gatcgtttag ttcgccaccc
      721 atcacattgc catctgcatc tttcataatg aaaagcaaat ctataactct tattgtaccc
      781 ttctgtgttg ctatggagag ttcctctagc acactaccat caaagttgtt acctttaaac
      841 ccaacaacta tataatctac tggtcctaac atatattacc tccattagct ttgttctagt
      901 tattattata ctcttgcatg caaggaaaag ctaagccata agcgttataa agagcataat
      961 aacgaagtgg aataaccccc ctggcttagc acgtgctatt aggatcttta attagtcgta
     1021 gagctgttgt cagtagtggt ggttgtatcc gatggtgttg taggtgatgc aggtgttgtg
     1081 ttggtagtgt cttctgtctt ttgtgtttgg ctttctgtaa tggtctgttg tgtagttttt
     1141 acagactctt gtagactatt gattgacttg ttgacagcat ctattgaatc gttaatcttt
     1201 tggttctgtt gatactgatt ataagctagc cctaaaatta taagtaaagc aattaccagt
     1261 aatactccta aaagaatact cagcccattt gatgattttt tacttgtagg ttccataatg
     1321 cctcccagcg tttatataac cattatactc ttaattgcta actatggctg tgattacact
     1381 cttctataca aggtggtgat gttttaaata aatttgtttt ttgtgagcca tctaaaagaa
     1441 aatgctcccc aaattggtgg taagaagaaa gtaaataatc tataagatac agtcattgca
     1501 taagctgaac tctggtctac gccagcacta actaacccgg ctgatatgat agcttcttgg
     1561 acaccaattc caccaggcac aggtgcaata ccgccaagca aagttgcact caccacaata
     1621 actaatgcag tcgctattga aatatctact ccgaatgcct gcgctgtaat aaccatagtt
     1681 gttcccaata cgagttgcga taacaaattg ctcccaaaaa gcgtaactag cctaataggg
     1741 tgttcagcga ggatagaaaa cgaccctata acatctttta atatattgat cagttttagt
     1801 ctaagagttt ttgcgttata aagtacaatt aagcctatta gaattattcc acctgcaata
     1861 gaaagcacta ttacccaatc tgtgttgata ttattcttta gattcaaatt tacgtcacca
     1921 aaaataattg caattaccaa aattgtcatc tgcactaaaa atccggtaaa gctgtctagt
     1981 gcatcctgtg ttattgctgt aacatatggg actttaaatt tctttagata ggcaacattg
     2041 gtagctaccc tactagcggt acttgggact gcaagaccaa taaactggat agcagattgc
     2101 aaaataagag ttggtttaaa aggtatcggt gcggtaactg ccgacatcat agatgttgca
     2161 tttggtacta aaatgagttg tgctacaaag aaagctagca tgaaccatac taaatcagcc
     2221 gattttatag cgttccaaac ttctgtaaaa tctatattgg taatttgctg tatgagcgca
     2281 tatgtcgcca gagccataag acccgtgagc aatagatcgc gttttgaaat gtttcttact
     2341 ttggttaggc taagtacact catatttagt agtctttttt accttatact ttagtctagc
     2401 acaccaaaat atttactgta cgattacaaa actatgctat atttgcagac acgtatagct
     2461 ttatagatgg catgattgta ttaaataaat tatttttata gcggtagtag aataaaaaac
     2521 tttgaatggc aattatttac agttagatag ttatatatac ttggtagttt ttaaagcaga
     2581 tttagatagt tgctcgttcg ggagatatcc acgagcctcc tgtatcaact tttcgtagaa
     2641 cttttttatt atttttaata catcttagaa tagacgtaag ctgaaccaca ttcataacat
     2701 aaaatataaa atacataatt ggcgaataca ggctcattct cattttttct ctattaggca
     2761 tgtgttcatc cggccaaact gtccaaagta catataacgt tatagttaca tatgcaccta
     2821 aaagggcaaa tgggtatgcc tttgcaatac taagataaat tatatacacg agaagtattg
     2881 gttctattag cagtacaagc tcaccaaaca gcgccatggg cagacgatac aaggttagca
     2941 tcctactata tttttcatca
//
LOCUS       DAOPLS010000081         2938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_38260_length_2938_cov_3.546306, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000081 DAOPLS010000000
VERSION     DAOPLS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38260_length_2938_cov_3.546306"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..699)
                     /locus_tag="PKV52_02410"
     CDS             complement(64..699)
                     /locus_tag="PKV52_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HPD99098.1"
                     /translation="MEPKLIAFDLNKTLIRENSWLNLNIAMGVSTAEDDILVRWSQQR
                     IITDAMGQQILCEIYKQRGDISYQAIWKILSNYTYVPHAKKVVALLQSRGYSVALVSG
                     AMDILVKAVAEELDIKHWRAANTFIFNEFDMLQRIDSVKNDAHYKAEMLLELCNELQC
                     TPKECLVVGDGANDIELFKLTGNGVTFTGSNIKHEARYIITGLDEILDIVV"
     gene            complement(778..1032)
                     /locus_tag="PKV52_02415"
     CDS             complement(778..1032)
                     /locus_tag="PKV52_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99099.1"
                     /translation="MAKTILNQKVDVTALYFTNKNGQLKSFPKRIEYDGGHHQFVESG
                     LQMLVQTTSGVIRIFEMTDGHNNFHLRQNPQKDWTLVATY"
     gene            complement(1571..2017)
                     /locus_tag="PKV52_02420"
     CDS             complement(1571..2017)
                     /locus_tag="PKV52_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99100.1"
                     /translation="MVSVSPLTNAVSDNIDKTIQDLEAGTSPALAIQGLLMRDVQAMF
                     HGGPEQGLHFVDAANAWFGDPKSSVATTYEDMHAAYFDGWMLIAEAVGRRFIDEHKAE
                     AVTFSQTALAENVVMQVINKVLELEDAVIYGAEVISAYFIAVTEPA"
     gene            2134..>2938
                     /locus_tag="PKV52_02425"
     CDS             2134..>2938
                     /locus_tag="PKV52_02425"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPD99101.1"
                     /translation="MKTALTFTITSRLKGTLARTGVIATPHGTIQTPAFIVGGTKATV
                     KALTPEQVKDLGGQSILANTYHLMLRPGADVVQRAGGLGAFMNYDGPTFTDSGGFQVF
                     SLGVAYKKGIDKVAHSQKGEAEAAKKSADQLAKVTEKGVHFRSHIDGQKLMMTAESSM
                     ELQHQIGADIHMAFDECPAPLADRTYIIDSLNRTHAWAERCLIRHKELNAEHAKNGEF
                     LQALYGIVQGARHEDLRKQSANFMVSRDFDGYGIGGVFEPGEIPTVVKWT"
BASE COUNT          825 a          616 c          640 g          857 t
ORIGIN      
        1 gaaactgcca tttaatatcc ttagctccac ccattttatg ctacggctat tatagcatct
       61 tacttatacc actatgtcta gaatttcatc taaaccagtg attatatacc ttgcctcgtg
      121 cttaatattt gacccagtaa aggtgacacc attaccagtt agtttaaata gttctatatc
      181 atttgcgcca tcaccaacca caaggcattc ttttggtgtg cattgtagtt cattacataa
      241 ttctaaaagc atttctgctt tataatgtgc atcatttttt acagagtcaa ttctttgtaa
      301 catatcaaat tcattaaata taaatgtatt tgccgcgcgc caatgtttga tgtctaattc
      361 ttcggctacc gctttaacca aaatatccat ggcgccagaa actaaagcaa cgctatatcc
      421 tctactttgt agtaaagcaa ccactttttt tgcgtgtggc acgtatgtgt aatttgacag
      481 tatcttccaa attgcttggt atgatatatc accacgttgc ttataaattt cacatagtat
      541 ttgctgaccc atagcatcgg tgattatgcg ctgttgggac catcgcacga gaatgtcatc
      601 ttctgctgtc gaaacgccca ttgctatatt taaatttagc caagaatttt ctctaatgag
      661 cgttttgttt aaatcaaagg cgattagctt tggttccatg catatactat agattgtttg
      721 tcatcaaaat aaaaaacgcc gcataaatat gcgacgattt tatacaagag cttgcagcta
      781 gtaggtagct acgagcgtcc agtctttttg tgggttttgg cggaggtgaa agttgttatg
      841 accatcagtc atttcaaaaa ttcggattac accacttgtt gtttgcacga gcatttgcaa
      901 gccagattct acaaactggt gatgtccacc gtcgtactca atacgcttag gaaagctctt
      961 gagttgccca tttttgttcg taaagtacaa agccgttaca tcgacttttt ggtttaatat
     1021 agttttagcc atattctttg tctccagact ttaaagtttt gtaaagccga acgggattgg
     1081 aggcgatagt aaccttataa caataccgta attttgaggt gatagattgg tttgttaaaa
     1141 atgcgatagt agagtgaaat atatctgtat gtatagaacg aaagtttcgc attttagcaa
     1201 ccaaggtgcg tatcaaaaca tggtagctgt tatagggtta ctatctaaat gttgatgatc
     1261 attacgcttg cgtaccgttc gtagctcgct agcgctaacc attgcaacaa atgaatatgg
     1321 tgtttgtagg gacgcttaac agccaattgg ctatcagtga gtgtgcaaac atcttgtcat
     1381 gcagcttgta aattaaatta gcagtttttg ccaaagtact cacatagcta cttatagtat
     1441 aaatggtata tgctacggat gcgaagactg tattgttgta gatttttagt attaaaaaaa
     1501 ccaccagaca aatggtggtt ggggtggggc ggtcaggacg gtaaagtcct aacgcgcccc
     1561 acccttgacc ctaggcaggc tcggtgaccg caatgaagta tgctgagatt acttcagcgc
     1621 cgtaaattac ggcatcttcc agctccagca ccttgttgat tacttgcatg acgacgtttt
     1681 cggcaagtgc agtctgtgaa aacgtcaccg cctctgcctt gtgctcgtcg atgaaacggc
     1741 gaccaactgc ttcggcaatg agcatccagc cgtcgaagta tgcggcgtgc atatcttcgt
     1801 acgtcgttgc gaccgagctt ttcgggtcgc cgaaccatgc attggccgca tccacgaaat
     1861 gaaggccttg ttctggccct ccgtggaaca ttgcttgcac atctcgcatt agcaatcctt
     1921 gaattgcgag agcaggcgaa gttccagcct ccaagtcttg gattgtcttg tcgatgttgt
     1981 cgctgacggc atttgtcagt ggactgacag aaaccatttt gcctccctaa agatctaagg
     2041 gcaccgggtt gtgccacttc taacgatata ttataccata tttgtcaatt agtgaatgag
     2101 acttctgctt atctgttaca atagtggttg attatgaaga cagctttaac gtttacaatt
     2161 acatcacggc tcaaaggcac gttagcgcga acgggtgtta ttgcaacacc ccacggcact
     2221 atacaaacgc ctgcctttat tgtaggtggc acaaaagcta ccgtaaaagc actcacccca
     2281 gaacaagtta aagatctcgg cgggcagtct attcttgcca acacctacca tcttatgctg
     2341 cggccaggtg cagatgtggt acaacgggca ggtggcctag gtgcatttat gaactatgac
     2401 ggaccaacat ttaccgatag tggggggttt caggtattta gcctgggtgt tgcctataaa
     2461 aaaggtatag ataaagttgc ccacagccaa aaaggcgaag cagaggcagc taaaaagtca
     2521 gctgatcagt tagcaaaggt aacagaaaag ggtgtacatt ttagaagcca tatagacggt
     2581 caaaaactga tgatgacggc agaaagctct atggagctac agcatcaaat tggtgcagat
     2641 attcatatgg catttgatga atgcccagcg ccattggcag accgtaccta cataatagat
     2701 tcgcttaacc gcacacacgc ctgggcagag cggtgcctga tccgtcacaa agaactaaat
     2761 gctgaacacg ctaaaaatgg tgaattttta caagcactat acggtattgt gcaaggtgct
     2821 cggcacgaag atttacgtaa gcaaagcgca aactttatgg taagcagaga ttttgatggg
     2881 tatggaattg gcggagtctt tgagccaggc gaaatcccca cagttgttaa gtggacca
//
LOCUS       DAOPLS010000082         2923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_38540_length_2923_cov_4.727336, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000082 DAOPLS010000000
VERSION     DAOPLS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38540_length_2923_cov_4.727336"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2923
                     /gene="dnaE"
                     /locus_tag="PKV52_02430"
     CDS             <1..>2923
                     /gene="dnaE"
                     /locus_tag="PKV52_02430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPD99102.1"
                     /translation="PVVVTCDAHYLKHEDQDAHEVLLCVGTGSYVSEEKRMSLKDFPL
                     HVEKPSEIIQRWGKDYPEVITNTKAVAERCDIEIELGNILIPQFPVPKEYTEKSYLHE
                     LVYKGLAERYAPKKSSHKEAKNQLPKEVISRAEYELDVIDSMGFNGYFLIIWDFVAWG
                     KKQGIMFGPGRGSAAGSIIAYALHITDLDPIKNDLLFERFLNPDRISMPDIDIDIQDN
                     RRGEVIEYCVAKYGDDRVANIVTYGRMAARAAVRDVARVLQVPYADADRMAKLVPPPQ
                     QGRHLPLATSIKENNDLKKEYTSNPQAKEVYDMAVRLEGTIRSHGVHAAGVVIAPNDI
                     VSYVPLEMAQKGVVSTQYPMGIIEELGLLKMDFLGLSNLTTIKNALRIIRKVYGVTID
                     INTLPLDDKKTYELLGKGDTTGVFQLESAGMKRYLKELKPTEFNDIIAMCALYRPGPL
                     KAGLVDMFVRRKNGKEAVSVPHKKFENALSSTYGTLVYQEQVMQISKEVCGFSGGEAD
                     TLRKAIGKKIREVMLKMQQKFIDGGVEHSGVPKDVMEKFWDDLMGFADYAFNKSHSAC
                     YALIAYWTAYLKAHYPAAFMAALMTSDYDDIDRLAIEITECKHMGITVLPPDVNESFH
                     EFSVVPHKQDEAPSIRFGMDAIKNVGHNAVENIIVARETYGQFNELADFLEHTNARQI
                     NKKVLESLIKSGALDRFGDRSALLESIDSIVAVSSKLSKEALDGQVDLFGAGEIAKVK
                     PEVEIVSTGLYQTHEYLQWERELLGLYLSSHPLESYDVILTEKAVPLATIRIDDDGKT
                     ATIGGSISDLRQITTKNGQAMAFVQVEDIVGDQIEVVVFPRVLEANSASIVKDKVVLV
                     KGRVNSKDKAGNAMAEPKLIADTFMLLSLDEAKAYQPTGVKTSLGKPKKTQSRPPIDT
                     PLPKTKRLYIRLQTHHTDDVLRDIKVILEQHLGNTETVLVVGDKKRPIRLSQKIKYSE
                     G"
BASE COUNT          958 a          558 c          656 g          751 t
ORIGIN      
        1 tacctgtcgt tgttacttgt gacgcgcatt acctaaaaca cgaagatcaa gatgcacacg
       61 aagtactgct ctgcgttggt acaggttcat acgtgagcga agagaaaagg atgtcactta
      121 aagattttcc tttacacgta gaaaaaccat cagaaataat tcagaggtgg gggaaagact
      181 atccagaagt tataaccaat acaaaagcag tcgctgaacg ctgcgatata gaaattgaac
      241 taggtaatat tcttattcct cagttcccag tgccaaaaga gtacactgaa aaatcatatt
      301 tacatgagct tgtttataaa ggtctcgccg agagatacgc accaaaaaag tcttcccata
      361 aagaagctaa aaatcaattg cctaaagagg taatttcgcg agccgaatat gagctagatg
      421 ttattgattc catgggattt aatggctatt tcttaataat ttgggatttt gtcgcctggg
      481 gcaaaaaaca aggcattatg tttggacctg gcagaggttc ggcagctggc tcaattattg
      541 catacgcctt gcatatcaca gatctggatc ctattaaaaa cgatttgcta tttgagcgat
      601 tcttaaaccc tgatcgcatt agtatgccag atatagatat tgacattcaa gataatcgcc
      661 gcggagaagt aattgaatat tgcgttgcta aatatggtga tgacagagta gcaaatatcg
      721 tgacatatgg acgcatggca gcgcgggcag ctgtccgtga tgtagcacgt gttttacaag
      781 taccctatgc cgacgccgac cgtatggcaa aactagtgcc tccaccacaa caaggccgtc
      841 acttgccact ggctacttca atcaaagaaa acaacgactt aaaaaaagaa tacacttcta
      901 acccacaggc aaaagaagtg tacgacatgg cggtgcgcct agaaggcacc attagaagcc
      961 acggcgtgca cgctgccggt gtagtgattg caccaaacga tattgtgtcc tatgtaccac
     1021 tagaaatggc tcagaaaggg gttgtatcta cacaatatcc aatgggtatt attgaagagc
     1081 ttggcctact aaaaatggat tttttggggc tttctaattt aacgactatt aaaaatgctc
     1141 taagaatcat tagaaaagtc tacggagtaa caatagatat aaatacctta ccgttagacg
     1201 acaaaaaaac atatgagtta ctaggcaaag gagacaccac cggcgtgttc cagctagaat
     1261 cggctggtat gaaacgatac ttaaaagagc tgaagcccac agaatttaac gatattattg
     1321 ccatgtgcgc cttgtatcgc ccaggacctt taaaagcagg cctcgtagat atgtttgtcc
     1381 ggcgaaaaaa cggtaaagaa gcagtcagcg tgccacataa aaagttcgaa aacgctttgt
     1441 catctaccta cggcacactt gtttatcaag agcaggtcat gcagattagt aaagaagtct
     1501 gtggctttag tggcggtgaa gctgatacgt tacgaaaagc gattggcaaa aaaatacgcg
     1561 aagttatgct caagatgcag caaaagttta tagatggtgg tgttgaacat agcggcgtac
     1621 ccaaagatgt aatggaaaag ttctgggatg atttaatggg atttgctgat tatgctttca
     1681 ataaatctca ctccgcatgc tacgcactaa ttgcatactg gacggcttat ttaaaagcac
     1741 actacccagc agctttcatg gcggcactca tgaccagcga ttacgatgat atagatcgat
     1801 tggcaattga aattaccgaa tgtaaacata tgggtattac cgtcttgcct ccagatgtta
     1861 acgaatcgtt tcatgagttt tcggtagtac ctcacaagca agatgaagca ccttctatac
     1921 gctttggcat ggatgccata aaaaacgtcg ggcacaatgc agtagaaaat ataattgttg
     1981 ctagagaaac ttacggtcag tttaatgaat tagcggattt tctcgaacac actaatgccc
     2041 gtcaaattaa taaaaaagtg ctagaaagtc tcataaaaag tggtgcacta gatcggtttg
     2101 gtgatcgaag tgcgctgcta gaaagtatag actcaattgt agcggtatca tcaaaattga
     2161 gtaaagaagc gcttgatggg caggttgatc tgtttggcgc tggtgagata gcaaaagtaa
     2221 agccagaggt agaaattgta tcaactgggc tgtatcagac ccatgaatat ttgcaatggg
     2281 aacgtgaact tcttgggcta tatttatcta gtcacccgct tgaatcatac gacgtaatat
     2341 tgaccgaaaa agcggtgcca ctcgccacga tacggataga tgatgatggt aagacggcaa
     2401 ctattggagg cagtattagt gatcttcgtc aaataactac caaaaatgga caagcgatgg
     2461 cttttgtaca agtagaagat attgttggcg atcaaataga agttgttgtg tttccgcgag
     2521 tactagaagc aaatagtgca agtatagtga aagataaagt tgtacttgtt aaagggcgag
     2581 tgaacagtaa agacaaagca ggcaatgcta tggctgagcc aaaattaatt gcagatacat
     2641 ttatgttact tagtttggac gaggctaaag catatcagcc gactggtgta aaaacgtcgc
     2701 ttggtaaacc taaaaaaacc cagtcacgtc caccaataga tacacccctc cccaagacaa
     2761 aacgtcttta cattcgtttg caaactcatc atacagacga tgtgctgcga gatatcaaag
     2821 tgattctaga acaacacctg ggtaatacag aaaccgttct ggtggtgggt gataaaaaac
     2881 gtcctatacg tctttctcag aaaattaagt acagtgaagg gtt
//
LOCUS       DAOPLS010000083         2915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_38681_length_2915_cov_3.080420, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000083 DAOPLS010000000
VERSION     DAOPLS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38681_length_2915_cov_3.080420"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKV52_02435"
     CDS             complement(<1..1101)
                     /locus_tag="PKV52_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD99103.1"
                     /translation="MIGRSRHTLPNWAPRLVGIVIASIVATIAAGYVLLQISNLLSWL
                     LVSLFLSFALEPIVNQLVHRGWSRNIATSVVLLAFGGMAVVLVAAMIPLFVQQVTEVV
                     KQSPDWLTSLLSTVNSVAGTNISSTDIIDKLSLSDQAVTNYVTNIANNVLGFGRQLLY
                     VLLQVLAILLFTFYFVSDGPYLRRVICSFLSPRQQKIVLTTWEIAIEKTGGFMVSRII
                     LGGISTVAHYIALTIIGVPFALPLAIWVGVVSQFLPIIGTYIAAALPLLVAVLQSPTD
                     ALVLMVFIVAYQQLENYILGPKIAAHTMELHPAVAFGAVIAGASIGGIIGAFVALPIA
                     AMLQEIAIVYLNRNEVIESKLTETTTDSVLKNK"
     gene            complement(1117..2418)
                     /locus_tag="PKV52_02440"
     CDS             complement(1117..2418)
                     /locus_tag="PKV52_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99104.1"
                     /translation="MPTKKSNRKPPQSQEIASLRAESKRLQQENDALKEQLVAITSKK
                     PKKSRKWYKNALTVLLLTVSVVSFMLFNVSSWVKNTILDTDTFVATMQPLISQPAVQE
                     NITQEISTRLFENVNVEEQLENVLPDQVAFLSGPLANQVVSVTEGRIQKVISSPQVYT
                     LWGEALGKAQTAVVGYIQNDNNDGVVDVNDVYTKISANVSSDDKLSFLFNRQLPPKVG
                     TITLAEIQWLPQARSYINMLSVAPPVFLATSLVTLLGAVLLSVKKRRLVIVFLVLSLV
                     MMFATIAALSLGNWQLGNSVNPENKGLAQAVYTTITEPLKNRTISYGTLLFAITIVAI
                     VSAPIAWIKRVRQYVDTTIVHLATKLLPTVSLPRWLIDFSAYISVISWTLFIALFVIV
                     GVRIPPDYNQVITGFAWATFASIVLYIVHVCVRVMNRPSAQ"
     gene            2533..>2915
                     /locus_tag="PKV52_02445"
     CDS             2533..>2915
                     /locus_tag="PKV52_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD99105.1"
                     /translation="MSSKVQSSPINQKVIITAVIISVICLMLFLQNYFILITLAAILA
                     FLFNPIYRWFLVKFKDRKGSAVSMTVAVMFITVAIPVSLLITVTVAQAIELVNNIQST
                     TSTGSFSDAVDNAVNSLNDQAARLP"
BASE COUNT          875 a          604 c          546 g          890 t
ORIGIN      
        1 tttgtttttt aacacactat cagttgttgt ttcggtaagc ttagattcaa tgacctcatt
       61 cctatttaag tatacaatgg ctatctcttg gagcattgcg gcaattggta gcgcaacaaa
      121 cgcaccaata attccgccga tgcttgcgcc ggctatcacc gcaccaaatg caacagccgg
      181 gtgaagttcc attgtatgtg ctgctatctt aggtcccaat atgtagtttt ctagctgttg
      241 atacgccaca ataaagacca tcaacacaag agcatctgtt gggctctgta ataccgccac
      301 caataacggt agtgctgctg caatatacgt acctataatt ggtaaaaact gggatacaac
      361 cccaacccat attgccagtg gtaaagcaaa tggcacacca attatagtaa gtgcaatata
      421 gtgtgctacg gttgatattc caccaagaat aatccgtgac accataaagc caccagtttt
      481 ttctatggca atttcccagg tagtaagcac gattttttgt tgtcttggtg acaagaaaga
      541 acaaatcacc ctacgtaaat atggtccatc agacacgaaa taaaacgtaa ataataatat
      601 cgctagcact tgtaacaata catacaacag ttgtcttcca aagcctagca cattgttagc
      661 tatgttagtt acataattcg ttaccgcctg atcacttaaa gataatttat ctattatgtc
      721 tgttgatgaa atgttcgttc ctgcgactga attaacagta gaaagtagag aggtaagcca
      781 gtctggtgat tgctttacta cctcagtcac ttgttgaacg aatagtggta tcatcgcagc
      841 aactagcacc actgccatac ctccaaatgc cagcaacaca accgatgttg cgatatttct
      901 tgaccagcca cggtgaacca gttggttgac aatcggctcg agtgcaaatg acaaaaatag
      961 cgaaacaagc aaccacgata gtaagttaga tatttgtagc aacacataac cagcagcgat
     1021 tgtagcaaca attgatgcga tgacaatacc aacgaggcgc ggcgcccaat tcggaagtgt
     1081 gtggcgtgat cttcctatca tagttctata gtaacactac tgggctgatg gtcgattcat
     1141 tacacgaaca catacatgta ctatgtatag cacaatgctc gcaaaggtag cccatgcgaa
     1201 tcctgtaatg acttgattgt aatcaggtgg aatccgtacc cctacaataa caaacaatgc
     1261 gataaataac gtccagctta ttacactgat gtacgcagaa aaatctatta accaacgagg
     1321 cagcgatacg gtcggtaaca gcttggtggc caagtgcaca attgttgtat cgacatactg
     1381 ccggacgcgt ttaatccagg caattggcgc acttacaata gcaacaatag ttatcgcaaa
     1441 taacagtgta ccgtaactaa tcgtcctatt cttgagtggt tctgtaattg tcgtatacac
     1501 agcttgggcg agccctttgt tttctggatt cacactattg cctaactgcc aattgcccag
     1561 cgaaagcgca gctatcgttg caaacatcat tactaatgag agtactaaaa atacgataac
     1621 taagcgtcgc ttttttacag ataaaagtac tgcaccgagc agggttacta aactagtcgc
     1681 taaaaataca gggggggcaa cactcagcat attaatgtaa ctacgtgctt gtggaagcca
     1741 ctgaatttca gcgagggtga ttgtgcctac cttggggggc agttgtctat taaacagaaa
     1801 acttaattta tcgtcagacg atacgtttgc agatattttc gtgtatacat catttacatc
     1861 cactactccg tcattattat cattttgaat atagccaacc acagcagtct gcgctttacc
     1921 caacgcttca ccccaaagtg tatatacttg tgggctacta ataacttttt gaatgcgtcc
     1981 ttcagttact gataccactt gattagcgag cggaccactt aaaaaagcca cttggtctgg
     2041 taagacattt tctaattgtt cttctacatt tacgttttca aacaatcttg ttgatatttc
     2101 ttgcgtaata ttttcttgaa cagccggctg actaataagt ggctgcattg ttgcaacaaa
     2161 tgtatcagtg tctagaattg tgttttttac ccaactagat acattaaaca acataaaact
     2221 aacaacagac acagtgagta ataacactgt taaagcattt ttataccact tgcgtgattt
     2281 ttttggcttt ttagatgtaa tggcaaccaa ctgctctttt agagcatcat tttcttgttg
     2341 taatctcttt gactctgccc taagagatgc gatttcttgt gattgtggtg gtttacgatt
     2401 acttttcttg gttggcatat ggcactactt cttgtaactt agctatattt aaaacatatt
     2461 atactacttt tttaatatta tttcaagttt ctttgccaaa aatgcttatt tctgtgtggt
     2521 atagttgtgc atatgagttc taaagtacaa tcctcaccca taaatcaaaa agtaatcatc
     2581 actgccgtta ttattagcgt gatatgctta atgctatttt tgcaaaacta ttttatatta
     2641 attacgcttg ctgcaatttt agccttttta tttaacccta tatataggtg gtttttagtt
     2701 aagtttaagg acagaaaagg tagcgcggtg agtatgactg tagccgtaat gtttattaca
     2761 gttgccatac ccgtttcact gctcatcacg gtaacggtag cacaggcaat agagttggta
     2821 aataatatac aatctactac ttctactggt tcattttctg atgctgtaga caatgcagtc
     2881 aatagtttaa acgatcaggc tgcaagatta ccatt
//
LOCUS       DAOPLS010000084         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_39315_length_2882_cov_3.551468, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000084 DAOPLS010000000
VERSION     DAOPLS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39315_length_2882_cov_3.551468"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..571)
                     /locus_tag="PKV52_02450"
     CDS             complement(176..571)
                     /locus_tag="PKV52_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99106.1"
                     /translation="MKRHVKRVIYDVVGILLILISPFLDWIPGPGGIPVFLAGLGLLA
                     VHNDWAKRILDWAKINADHLLASLFPRKKSIERVHDAIGILLIILSGVAFYYLTPPLQ
                     YILAIALLSSATFWLLYNRRRYHWFIKKH"
     gene            complement(665..1495)
                     /gene="pheA"
                     /locus_tag="PKV52_02455"
     CDS             complement(665..1495)
                     /gene="pheA"
                     /locus_tag="PKV52_02455"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPD99107.1"
                     /translation="MKKIAIQGQAGSFHHVAACSVYGEDIAIVACDTFKSVFTTLHQG
                     QADAAIVAIENSLYGSINEVYDLLLEYPFAIVGEVEEKIHQCLITLPGVHISDITAVH
                     SHPVALSQCTNFLDQKLPQATRYERYDTAESVTYIKRLQDPKHAAIASSASARMHNLP
                     IAKSNIQNHQSNSTRFFVIDAKKNNYSSTNKASLVIETTHTPGALYEALGVFKSYNAN
                     LTKLQSRPIQSTVWRYMFYVDIETTPQNMPKIIDSLQAKGCKTILLGMYTKNLTTVNA
                     "
     gene            1560..>2882
                     /locus_tag="PKV52_02460"
     CDS             1560..>2882
                     /locus_tag="PKV52_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPD99108.1"
                     /translation="MQEFIKVKGAREHNLKNISIDLPRNKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGLMEKPDVDQIEGLSPAISIDQKSTSRNPRSTVATV
                     TEIYDYLRLLYARIGIPHCPECGKAVNRQTIPAVVDLIHSSRMDKRILVLAPIVVDKK
                     GAFEHIPEQFQRAGFARARVDGVVYALDEFPVLDKAKKHSIDIVVDRLVVSQDVISRS
                     TQSIEQAFEIGDGKIIIHDVDNKEDIQYSLLYACVDHPDIAIPELEPRTFSFNSPHGA
                     CPVCTGLGNRLEVDPELVLPNGRLTIAEGAIRPWNRIRIDSWYLKKVEAMAEANGFSI
                     HVPTGTLSAKDLEKVLYGTGSQVYQVPLSGGRSYETTFEGVIPNLERRHKETESEFIK
                     KDIEKFMLQQPCHACKGARLKPAVLAVTVKNMSIIDVVNLSIDDAIEFF"
BASE COUNT          900 a          540 c          597 g          845 t
ORIGIN      
        1 caggaataat acctagtaca attaactcaa aaaagggttt ataggcgacg gtgtagtggg
       61 gcagcatgtg aaaccctgca agcagtataa taatttgtgc agaattgtag taaagccacc
      121 agatcatatt tgtaaagtat cataaatttt acaaaaaatc aatagtagta ataagctagt
      181 gtttttttat aaaccaatgg tacctacgtc tgttgtacag aagccaaaag gtagcacttg
      241 atagcaaagc tattgctaaa atgtattgca gtggaggggt gaggtaataa aaagcaacac
      301 cacttaaaat tataagcaat atacctattg catcatgcac gcgttcaatt gattttttgc
      361 gtggaaacag tgaagcgagc agatgatcgg catttatctt agcccaatct agaatacgtt
      421 tcgcccaatc attatgcacc gctagcaagc ctaatccagc aagaaacaca ggtataccac
      481 ctggtcctgg aatccagtct aaaaaaggac taataagtat caacaaaatg ccaacaacat
      541 catatattac gcgtttaacg tgtcttttca ctatagtatt gtaccaatta gtacttgatt
      601 tctactacga tagtcgttgt gtattacata tgatgtacag ttgctaattt aataacccta
      661 cgacttatgc gtttacagtt gttaaatttt ttgtgtacat acctagcaaa atcgttttac
      721 aaccctttgc ttgcaatgag tcaataattt ttggcatatt ttgtggtgtt gtttcaatat
      781 ctacatagaa catatagcgc caaacggtag attgaattgg tcgtgactgt agtttagtaa
      841 gattagcatt gtatgattta aaaactccga gtgcttcata caacgcccct ggtgtgtgag
      901 tggtttctat aacaagtgat gctttattag tactactata attgttcttt ttagcatcaa
      961 taacaaaaaa acgagtagag ttggattgat gattttgtat attagatttg gcgattggta
     1021 aattatgcat gcgggctgag gcgctactcg caatagctgc atgcttaggg tcttgtaacc
     1081 gttttatata cgttacactt tcagccgtgt catatcgttc gtaacgagtt gcctgcggca
     1141 gtttctgatc gagaaaattc gtacattgtg aaagcgcaac cgggtgagag tgtacggctg
     1201 tgatgtcaga aatatgtaca cctggcagcg taataagaca ctgatgaatt ttttcttcta
     1261 cttcgcctac gattgcaaaa gggtattcaa gaagtaaatc gtatacctca ttaatagacc
     1321 catatagtga gttttctatt gctacaatcg ctgcatctgc ctggccttgg tgtagcgtcg
     1381 taaatacaga tttaaaagta tcgcacgcaa cgatagcaat atcttcacca tagactgaac
     1441 atgccgctac atggtgaaac gatccagctt ggccttgtat ggcaattttt ttcatacttt
     1501 ccatatagta ctataagtat ctagaaaatt agatataatt ttgttacaat tgatggtcga
     1561 tgcaagagtt tataaaggtt aaaggtgccc gtgagcacaa tttaaaaaac atatctattg
     1621 acctgccacg aaataaactc gtcgttatta caggtttgtc aggatcaggt aagtctagtc
     1681 tcgcctttga taccatttac gcagaaggcc aacgtcgcta cgtagaaagt cttagtagtt
     1741 atgcacgaca gtttctaggg cttatggaaa aaccagacgt agatcaaata gaaggtttaa
     1801 gcccagctat atcaatagat caaaaatcaa ctagccgtaa tcctcgttcg acagtagcca
     1861 ctgtcactga aatatatgac taccttagac tgctttacgc aagaataggt atacctcact
     1921 gtccagagtg cggcaaagca gtgaaccgcc aaacaattcc tgcagtggta gatttaattc
     1981 attcttcacg aatggataaa cgtatattgg tattagcacc aattgtcgta gacaaaaagg
     2041 gagcgtttga gcacatccca gagcaatttc aacgagcagg ttttgcccgt gctcgtgttg
     2101 atggtgttgt ctatgcttta gatgaatttc ctgtgctaga taaggcgaaa aaacacagta
     2161 tagatattgt tgtcgatagg ctggtggtga gtcaagacgt cattagcagg tcaactcaat
     2221 ctatcgaaca agcatttgaa attggtgacg gcaagataat aatacacgac gtagataata
     2281 aagaagacat tcagtattca ctactatatg cttgcgttga tcatccagac atagcgattc
     2341 cagaactaga gcctcgtacg tttagtttta attcgccaca tggtgcatgc cccgtgtgta
     2401 cagggctagg taataggctt gaagtagatc ctgaacttgt tttgccaaac gggaggctta
     2461 ccattgccga aggtgcgata cggccctgga accgtattcg catagacagc tggtacttaa
     2521 aaaaagtaga agccatggcc gaagcaaacg gtttttctat acacgtacca actggaacgc
     2581 tcagtgctaa agatttagaa aaagtgcttt atggcacagg ttctcaagta tatcaggtgc
     2641 cactcagcgg tggtcgctcg tatgaaacaa cctttgaagg cgttatccct aacctagaac
     2701 ggcgccacaa agaaacagag agcgaattta ttaaaaaaga tattgaaaaa tttatgctcc
     2761 aacagccgtg ccatgcttgt aagggcgctc gtttaaagcc agcagtatta gctgttaccg
     2821 ttaaaaatat gtccatcatt gatgtagtaa atctatctat cgatgatgcg attgaatttt
     2881 tt
//
LOCUS       DAOPLS010000085         2837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_40211_length_2837_cov_4.207045, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000085 DAOPLS010000000
VERSION     DAOPLS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40211_length_2837_cov_4.207045"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKV52_02465"
     CDS             <1..352
                     /locus_tag="PKV52_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99109.1"
                     /translation="RALPTESFGAAGSLTHPTSNRPFESVVMAVDDITCTALSAVSLK
                     KTSDFMLTGSSAALAEFVMNPPKPIITARAAMKLNIFLIIDLPSFKGCLGGTSATRPL
                     WARFIIYSYLCYTQ"
     gene            complement(725..>2837)
                     /locus_tag="PKV52_02470"
     CDS             complement(725..>2837)
                     /locus_tag="PKV52_02470"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD99110.1"
                     /translation="AITVLASLKKQPYKYVFLSVLATVILWAVGDILMLTSDSTTTAY
                     FGAQLFYIAPLFTPVLLWLFAIQFPENSRLKKIHIYSSVVALLLATIPLLINVNFIIY
                     PIELAEPFNKINPRNPGFLLYGLFLIVFFLLVYITLLQKIRTLKGTARLQVLYTYFGV
                     VLSSSLALVTNVAMPLLGYVTYVWLGPPFTLINTIAMFMAMFKYQLFEFKFVVLRLIS
                     YTFLILFISVTYTMIVVLLSASLTQSALQFDKNFYVTVIATLLVVFTVGNLRSKFDKY
                     TNKIFFKDAYNSQELINQINSLLVSKNQLHDLIGRIALLIENNIKVTFCNYYINAAAT
                     VDFHIAGSNTTLFSKHEWHEVLEYVSGVSKKVIRIDDEDLPDTVKVAFKALGIELCVQ
                     MISSDVDVGYIIIGEKRSGNDYTKEDMQLLEIIADEVAIAVQNNLQFNEISQFNITLQ
                     KKIEEATKELQKSNEKLQKLDEAKDEFISMASHQLRTPLTSVKGYISMILEGDAGPVN
                     DTQKRFLDQAFLSSQRMVYLIADLLNVSRLKTGKFVIEANPTYLPDVVESEINQLQET
                     AKARELELVFTKPAEFVTLNLDETKIRQVIMNFVDNAIYYTPRGGRITVALKQLKNSI
                     EYTVTDTGIGVPKKDQHHLFTKFYRAANAKKSRPDGTGLGLYMAKKVVIAQGGAMIFK
                     TTEGKGSTFGFTFPIPETANE"
BASE COUNT          803 a          577 c          555 g          902 t
ORIGIN      
        1 ccgcgcctta cccaccgaga gctttggagc ggcagggtcg ttgacccacc ccaccagcaa
       61 ccgacccttc gagtcagttg tgatggctgt ggatgatatt acatgcactg cgctgtcggc
      121 ggtttccttg aaaaaaacct ccgacttcat gctgaccggg tcttcggcgg cgctggcgga
      181 gttcgtaatg aaccccccca agccaatcat caccgccaga gcggcgatga agctaaatat
      241 cttcctgatc atcgatctgc cctccttcaa ggggtgtctt ggcggaactt ccgccacacg
      301 ccccttatgg gcgcggttta ttatttacag ctatctgtgc tatacgcaat aaaccacgcc
      361 cattaggagg tcataacctg tagtagttag tgtcatatgg gcattcggcc acaagcttaa
      421 aaataagctt gggctgcaaa agacccaaat gactatctag tttgctttgt ttacgttatt
      481 tgtcttgtta tagacaacaa agcatatgtc taaattgtta agcatcacca agcaaaccgg
      541 acccacctcg ctcgttgatt ggattagtaa cataatatga cattattgac tttttgtcaa
      601 tagctataag caatatgttt ttggttatgc ttacccttgc tatgttttgc cgtacaggca
      661 ctttgcatca ccttgtaaca ttggtttata taagttacca gacagcctac tatatggtgt
      721 atttttattc gttagcagtt tctggaatag ggaacgtaaa gccaaaggta ctgcctttgc
      781 cctcggtagt tttaaatatc attgccccgc cctgtgcaat taccactttt ttggccatat
      841 acagccctag gccggtgcca tctgggcgtg attttttagc attcgccgcg cggtaaaact
      901 tggtaaacaa atggtgttgg tctttttttg gcacgcctat acctgtatct gttactgtgt
      961 attcgatgct attttttagt tgctttaggg caaccgttat acggccaccg cgcggcgtgt
     1021 agtaaatagc gttatctaca aaattcataa tcacctgccg aatttttgtt tcgtctaggt
     1081 ttagggttac aaattcagct ggcttggtaa acaccagctc taattcacgg gccttggcgg
     1141 tttcttgtag ctggtttatt tcgctctcga caacatctgg taaataggtt gggtttgctt
     1201 ctatgacaaa tttacctgtt tttaagcggc tcacattaag taaatcggca attaaatata
     1261 ccatacgctg gctacttaaa aatgcttggt ctaaaaagcg tttttgggtg tcatttacag
     1321 ggccagcgtc gccttctaaa atcatgctaa tgtagccttt tacgctagta agtggcgtgc
     1381 gtagctggtg tgatgccata ctaataaatt cgtctttggc ttcatcgagc ttttgcagct
     1441 tttcgttgct tttttgtagc tctttggtcg cttcttctat ttttttctga agtgttatgt
     1501 tgaactgact aatttcatta aattgtagat tattttgcac cgcaatggct acttcatccg
     1561 cgataatctc aagaagttgc atatcttctt ttgtgtagtc attaccactt cttttttcac
     1621 caataatgat atatccgaca tcgacatcac tagatatcat ttgtacacat agctctatac
     1681 cgagcgcctt aaatgctact ttgacggtat cgggcaaatc ctcgtcatct atacgtatta
     1741 ctttttttga aacaccactg acatattcta aaacttcatg ccactcgtgc ttagaaaaca
     1801 atgtcgtgtt tgacccagct atatgaaaat caactgttgc tgctgcattt atatagtaat
     1861 tacagaaagt gacttttata ttgttttcga ttaatagcgc aatccgacct ataagatcat
     1921 ggagttgatt tttagatacg agtagtgagt ttatttggtt aatgagctct tgggaattgt
     1981 aggcatcctt aaagaatatt ttatttgtat atttatcgaa cttagagcgt agattgccta
     2041 ctgtaaatac tacaagcagt gttgctataa ccgtgacata aaagttttta tcaaactgga
     2101 gggctgattg tgttaaacta gcgctcaaca gaacaacaat catggtatat gttacggata
     2161 tgaacaggat caagaatgta tagctgataa gccttagaac gacaaatttg aactcaaata
     2221 gctgatattt aaacatcgcc ataaacattg caattgtatt aatgagcgtg aagggcggcc
     2281 caagccacac atatgttaca tagcccaata atggcattgc aacattagtt acaagtgcca
     2341 ggctgctcga caacactacg ccaaaatatg tatataacac ttgtaatcga gccgtacctt
     2401 taagcgttct aattttttgt aataaagtaa tataaacaag taaaaagaaa actattaaaa
     2461 acaaaccata caatagaaag cctgggtttc gtggatttat tttgttaaaa ggttctgcta
     2521 attctatagg ataaataata aaattcacat ttattagcaa aggaattgtt gcaagtaaaa
     2581 gggctactac actactatat atatgaattt tttttaatct gctattttct gggaattgaa
     2641 tagcaaataa ccataaaagt acaggggtga ataacggtgc gatataaaat aactgtgccc
     2701 caaaataagc ggtcgtcgta gaatctgacg ttaacataag tatgtcaccc acagcccata
     2761 atatgactgt cgctaatacc gacagaaaga catatttata aggttgcttc ttcagagaag
     2821 ctaacacagt aattgca
//
LOCUS       DAOPLS010000086         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_40757_length_2810_cov_3.761162, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000086 DAOPLS010000000
VERSION     DAOPLS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40757_length_2810_cov_3.761162"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKV52_02475"
     CDS             <1..869
                     /locus_tag="PKV52_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPD99111.1"
                     /translation="DEIHRFNKAQQDAFLPHVEKGTITLIGATTENPSFEVIAPLLSR
                     MRVVVLEPLADADIIRIAKKALKILGYAHAVPKQAYQTLAHIAGGDARVALGTLELAL
                     SLSPTISMETIKQAAQTTMPSYDKAGEQHFAMVSAFIKSMRGSDVNATLYYLARMLQA
                     GEDPKFIARRMIIFASEDVGVAASPALNLAVSTFLAVERIGMPECEYSLYHCATVLAG
                     SKKSRAIPNAMGKAKQAAVATPHLHVPLHLRNASTSLMKELGYGKSYQWEADYKHPDG
                     FLPPEVKDLQVF"
     gene            complement(917..1324)
                     /locus_tag="PKV52_02480"
     CDS             complement(917..1324)
                     /locus_tag="PKV52_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99112.1"
                     /translation="MAKVSTKQEVSGWVGWVAFAGVLSMVLGTFQAVAGIVALFKNEV
                     YVLGPENLWLFDYTTWGWVHLLWGIFLVLAGGAILSGKTWGRVTGVILASVSALVNFA
                     FIPIYPLWSIVIIALCVFVIYALIVHGDEIAVE"
     gene            complement(1434..1619)
                     /locus_tag="PKV52_02485"
     CDS             complement(1434..1619)
                     /locus_tag="PKV52_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99113.1"
                     /translation="MTPSTNGYAQTSQMIEVILDDFGFFKNSYHEPKPAFVDNTFTTT
                     AIKSTIYPAKKKFKTTI"
     gene            complement(1651..2466)
                     /locus_tag="PKV52_02490"
     CDS             complement(1651..2466)
                     /locus_tag="PKV52_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPD99114.1"
                     /translation="MMTLFHKLFHVPYSLKITQDTKQGLPVIFLHGVASDSSTWKHVL
                     PKLPPHYRAITIDLLGFGSSPKPSHISYTMQDHARAVTKTIRRLGMNKPAVIVGHSMG
                     ALIAIDIAKNNPQLVHSLVLVGAPLYNPEDIVFATSKYANPKLSLGNSLFYMYDQIVY
                     NKKLTLKTAQRFVKIAPFRNSFILTKDTWEPFKLSLTNTIMKQNSTAELLGLHSKVVL
                     VYGKMDLLLQQKNYDKLASFHNENIILMPHRGAHMITKQSAPLVIQAIEEAAK"
     gene            2528..>2810
                     /locus_tag="PKV52_02495"
     CDS             2528..>2810
                     /locus_tag="PKV52_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99115.1"
                     /translation="MKQLIRMLSVAGLTGVLVAGIYFVFSAVVEQLQDFIWFTLIDTE
                     ENKLLVFPLAITAGVLFGFLLKILGEDTKHEANHSIASYASSASQINSSW"
BASE COUNT          820 a          563 c          594 g          833 t
ORIGIN      
        1 tagatgaaat tcatcgtttt aacaaagctc aacaagatgc atttttacca catgtagaaa
       61 aaggcacaat cacactaatt ggtgcaacga cagaaaaccc tagctttgag gttatagcac
      121 cgttgttgtc acgcatgcgt gttgttgtgc tagaaccgtt agcagatgct gatataatac
      181 gaatcgcaaa aaaagctctt aaaatacttg gttacgctca tgcagtgcca aaacaagcct
      241 atcagacact tgcgcatatt gctggtggtg atgctcgggt tgcgcttgga acactagagc
      301 tagctctaag cctatcccct actatttcta tggaaacaat aaagcaagct gcccagacca
      361 ctatgcctag ttacgacaaa gctggcgagc aacactttgc tatggtgagt gcatttatta
      421 agagcatgcg aggatctgac gtaaacgcaa cgttgtacta cctagctcgc atgctacagg
      481 ctggtgaaga cccaaaattt attgctcgca gaatgattat atttgcaagc gaagacgtag
      541 gtgtggcagc tagtccagca cttaatttag ctgtgagtac atttttagca gtcgagcgaa
      601 ttggtatgcc ggaatgcgaa tatagcttgt atcactgcgc aaccgtattg gctggttcaa
      661 aaaaatctcg cgcgatacca aatgctatgg gaaaagctaa acaagctgca gtagctaccc
      721 cccacctaca cgttccgttg cacctgcgta atgcgagtac ttcgcttatg aaagaattgg
      781 gatacggaaa gtcatatcaa tgggaagctg attacaagca ccccgatggg ttcttgccac
      841 cagaagttaa ggatttacag gtattttaat aagcagcctg tttaggggct gcttattaac
      901 acatttcact atagagttat tctacagcta tttcatcacc gtggacaatc agtgcgtata
      961 tgacaaacac acaaagtgcg ataatcacaa ttgaccagag tgggtagatt ggtataaatg
     1021 caaagttaac cagtgcactt acgcttgcaa ggataacgcc cgtaacacgt ccccatgttt
     1081 tgccagataa gatagcacca cctgctagta ccaaaaagat accccacagc agatgtaccc
     1141 agccccatgt tgtgtagtca aacaaccaga gattttctgg tcctaaaaca tacacctcgt
     1201 ttttaaacag cgctacaatg ccggcgacag cctgaaatgt accaagcacc atgcttaata
     1261 caccagcaaa ggctacccaa ccaacccagc cactgacttc ttgttttgta cttactttag
     1321 ccataaaacc tccttgtttt atagttgaat attacaccaa aacgtaacta caaacaaata
     1381 ttatgattaa gatgtagtgc aagatacagg tgtatttaaa tggtatactg tttttatatg
     1441 gtagttttaa acttcttctt agcaggatat attgtgcttt taatagcagt tgtggtaaat
     1501 gtattatcca caaaagctgg cttgggttcg tggtatgaat ttttaaaaaa cccaaaatca
     1561 tctaagataa cttcaatcat ttggctggtc tgggcgtatc cgttggtact tggtgtcata
     1621 gcctattttg ccgtcagtgt tgcgttttaa ttatttagcc gcttcttcaa tagcctgtat
     1681 aaccaatggt gcactctgtt ttgttatcat atgagcacct ctatgtggca taagtattat
     1741 gttctcatta tgaaaactcg ctagtttatc gtaatttttt tgttgtagta ataagtccat
     1801 tttaccgtag acgagcacca ccttactatg caaaccgagc aattctgccg tactgttttg
     1861 tttcatgatg gtgttggtaa ggctgagttt aaatggctcc catgtgtctt ttgtgagtat
     1921 aaaactgtta cgaaacgggg caattttgac aaagcgctgg gcagttttta gcgtgagttt
     1981 tttgttgtag acgatttggt catacatata aaatagacta ttaccgagac tcaattttgg
     2041 attggcatat ttactcgttg caaaaacaat atcttctgga ttatacagcg gtgcgccaac
     2101 cagaaccaga ctatgtacga gctgaggatt atttttagca atatcaatcg caattagggc
     2161 acccattgag tgacctacaa tgacggccgg tttattcata ccaagacgac gaattgtttt
     2221 cgtaacagca cgggcgtgat cttgcattgt atacgatata tgactaggct ttggcgaact
     2281 accaaaccct agtagatcta ttgttattgc tctgtagtga ggcggtagtt taggtagaac
     2341 gtgcttccac gtagagctgt cactagctac tccgtgtaaa aaaataacag gcagaccttg
     2401 tttggtgtct tgcgttatct taagggaata aggaacgtgg aatagcttgt gaaatagtgt
     2461 catcatcata ttctagtata cggtattttt gtcagaggtg aagaaagtat gctacgctaa
     2521 tggtaagatg aaacaactca tacgcatgtt gtctgttgct ggtttaactg gcgtgctcgt
     2581 ggccggtata tattttgtat ttagtgccgt cgtagaacag ctgcaggatt ttatatggtt
     2641 tactcttata gataccgaag aaaataaatt actcgtgttc cccttagcta tcacggcggg
     2701 tgtactcttc ggctttttgc ttaaaatatt aggcgaagat actaaacatg aagctaatca
     2761 cagcattgca agttatgcat ctagtgcaag tcagattaac agcagctggg
//
LOCUS       DAOPLS010000087         2806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_40838_length_2806_cov_3.356961, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000087 DAOPLS010000000
VERSION     DAOPLS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40838_length_2806_cov_3.356961"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKV52_02500"
     CDS             <1..217
                     /locus_tag="PKV52_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99116.1"
                     /translation="QPHLEHASKKQPLTSYTLKDELPDSETWWQQIGLEPTGTQEVYP
                     FGPDTRPSTEVRMQASSVKSVLHMLKR"
     gene            complement(241..1890)
                     /locus_tag="PKV52_02505"
     CDS             complement(241..1890)
                     /locus_tag="PKV52_02505"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HPD99117.1"
                     /translation="MQSVINILVEHQLLLLFVVITLGYAIGRINIFGIKLGAAAVLFV
                     GLFLSALDDRLSLPAIITTLGLVLFVYSVGVSSGASFFGSFKKFGLKDLALVSAFLFF
                     AAGLIATLTSYSGINGAISTGIFTGSLTNTPALAASINLLESGQFSESGIVPTTPTIG
                     YSIAYPLGVIFPIVAIFFWQKRFRIDYKKDIHKAKGLVSVGADIKNKAICITNARYIG
                     QSIRQLKDDNNWPVFFVRIKRSGKIHIISDLSTKIKRGDILSVIGTGKDIDVVAKALG
                     SYTDDVLQFETSNYIYRRVFVSEKNIIGKTIADLHLPKRYGAIVTRIRRGDADLVADS
                     NHVLEYGDRIRFVSPKEYEHKVAKVFGDSYRSISDIHPMSIGLGICLGLLVGMIPIHL
                     PGGIEFELGEAGGPLVVGLILSYLRRTGPFVWTISYSANHTIRELGLSLMLAGIGVRS
                     GQTFINGIQDGNGLQIFLIGGIISFIVPMIFIPLAYKKFKLPFAVVSGMVSAIHTQPA
                     VTGYASQQAKNDLPTYGYVRLFPLATIMKIIVAQIMLLALV"
     gene            2107..>2806
                     /locus_tag="PKV52_02510"
     CDS             2107..>2806
                     /locus_tag="PKV52_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD99118.1"
                     /translation="MTQQEALAIMDSGASVLLTGAAGTGKTHTLNVFIRHARASGKLV
                     SVTATTGLAATHLNGNTIHSWAGIGIKHQVHPDMLKKMPDSRKKIIKNTDILIIDEIS
                     MLHDYRLEMIDTVCKHVRQNDAPFGGLQVILCGDFYQLPPVNRADEPHGGFITQSSLW
                     HDGIFSVCYLVEQYRQKDDQVYTDILNGIRAGVLNRSQLTKLLDKQNTTLDATLAVTK
                     LLTTNVDTDSINQQH"
BASE COUNT          921 a          589 c          537 g          759 t
ORIGIN      
        1 gcagccacac ttagaacacg ctagcaagaa acaaccactc acatcatata ccctaaaaga
       61 tgagttgcct gattcagaga catggtggca gcaaataggc ttagaaccta cgggcacaca
      121 agaagtatac ccatttggtc ccgatacgcg accaagcaca gaagtgcgta tgcaagcatc
      181 gtccgtcaag tctgttttgc atatgctaaa aaggtgatgt aatttgtgct gtacgtagac
      241 ttacacaagt gcaagtaaca taatttgagc gacaataatt ttcatgatag tggcgagcgg
      301 aaataatcga acgtagccat aggttggcag atcatttttt gcttgctgcg aagcgtagcc
      361 cgtgacagct ggctgtgtat gtatagcaga taccatccca ctaactacag caaaaggtaa
      421 tttaaatttt ttataggcga gcggtataaa aatcatagga acaataaatg agatgatgcc
      481 tccaataaga aaaatctgca aaccattacc atcttgaata ccgtttataa acgtttgtcc
      541 agatcgcacc ccaatacctg caagcatcaa agacaggcca agctcacgaa ttgtgtggtt
      601 agcactgtac gatattgtcc agacaaaagg tcctgttcgg cgtaaataac tcaaaatcaa
      661 ccctactact aaaggaccac cggcttcacc tagttcgaac tctataccac cgggtaaatg
      721 aataggaatc atacccacca acaaacccaa acatatacca agaccaatgg acatcgggtg
      781 gatatcacta atagaccggt aggaatcccc aaagaccttt gctactttat gctcgtattc
      841 tttaggcgaa acaaaccgta ttctatcacc gtattcgagc acgtgattac tgtctgcaac
      901 taagtctgca tcgccacgac gtatgcgtgt aacaattgca ccataccgct ttggcaaatg
      961 taaatctgcg attgtcttac caataatatt tttttctgat acaaacactc tgcgatagat
     1021 gtaatttgat gtttcaaatt gtaaaacatc atccgtgtac gaacctaaag ctttagcaac
     1081 tacatcaata tcttttccag tgccaatgac gctcagtata tcgccacgtt taatctttgt
     1141 agataaatca ctaatgatat gtattttgcc agaccgtttt atgcgcacaa aaaatacagg
     1201 ccaattatta tcatctttaa gctgacggat tgattgacca atgtatctag cgtttgtaat
     1261 gcaaatggct ttatttttta tatctgcgcc gacactcact aaaccttttg ctttatgtat
     1321 gtcttttttg taatctattc taaaccgttt ttgccaaaaa aagattgcta caatcgggaa
     1381 aattacacct aaagggtatg caatagaata gcctatagta ggagttgttg gtacaatacc
     1441 actttcgctg aattggccag attctaataa gttaattgat gccgctaggg cgggagtgtt
     1501 cgtgagtgat ccggtaaaaa taccggtact tattgcaccg tttataccac tataactagt
     1561 aagggtagcg attaaaccag cagcaaagaa caagaaagca gatactaaag ctaggtcctt
     1621 taatccgaac tttttaaatg aaccaaagaa actagccccg cttgatacgc ctactgaata
     1681 gacgaataat actaaaccaa gtgtcgtaat aatagcaggt aaactaagcc gatcatctag
     1741 tgcgctcaaa aaaagcccga caaataatac tgcagcagct ccgagcttga tgccaaaaat
     1801 attaattcgt ccgattgcat agcctagtgt tatgacgaca aataaaagta gtagttgatg
     1861 ttcaaccaat atgttgatga ccgattgcat atgtctccgc tttggtttgg tcttgattgt
     1921 ctgtgtataa gtatagcgta ctacgaggct acgaacgaaa cgtatagctc ttatacaccc
     1981 agattaatta gtagtcgcct tagtttgcag ctagaataga gtatgtgaat acaatacaat
     2041 acaaaacaga atgttataca tgcccaacac gacaatatat tgggtaggtg aaaaggcagg
     2101 ctccttttga cacaacaaga agcactcgcg attatggatt ctggtgcgtc ggtacttctt
     2161 accggtgctg caggtacagg caaaacacat acactcaatg tctttattag gcatgcccgt
     2221 gcctcaggta aattagtttc tgtcacagcc acgacagggc ttgccgcgac tcatctgaat
     2281 ggtaatacaa ttcattcatg ggctggcata ggtatcaagc atcaagtgca tccagacatg
     2341 ttaaaaaaaa tgccagatag tcgtaaaaaa ataattaaaa atacggacat attaataatt
     2401 gatgaaatca gcatgctgca cgactatcgt ctagaaatga tcgatactgt ttgtaaacac
     2461 gtccgccaaa atgacgcacc attcggcggt ttacaagtta ttttatgtgg tgatttttat
     2521 caactaccac ccgttaatcg tgcagatgag ccacacgggg ggtttataac acaatcttca
     2581 ttatggcacg atggtatatt tagcgtgtgt tatcttgtag aacagtatcg tcaaaaagat
     2641 gaccaagttt acactgatat tttaaatggt atacgggcag gggtactcaa ccgtagccaa
     2701 cttacaaagt tactagataa acaaaatacc acgctagatg caaccttagc tgtaacaaaa
     2761 ctacttacaa caaatgtaga taccgacagt attaatcaac agcacc
//
LOCUS       DAOPLS010000088         2777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_41421_length_2777_cov_3.400808, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000088 DAOPLS010000000
VERSION     DAOPLS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41421_length_2777_cov_3.400808"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1553)
                     /locus_tag="PKV52_02515"
     CDS             complement(33..1553)
                     /locus_tag="PKV52_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99119.1"
                     /translation="MSTYGEGTPLSHTIEQVSTTEQDNNQRRWRIGRWFGSVALSVLA
                     SMGATQQNNSAPEHTGFTTVATMQTRSHEASIGIGLPDILPGDATLRLPGVEIAEQPD
                     DAKDTKSGTPESSQEIVKEQLRVALMEWARLFDKDAMAEKPVAMAQVQKIADTITDLI
                     QNDSEITDIRFIGSASDEDDYAYMYGGENPGLGIDSAKNEALALTRAKAVEKLVTALL
                     RQQLSAEEYEQVIDVMQKPKGIETQDKQLNKDIEQLADTMGIRTVDLVSLYNRNPQEL
                     PKDARETLAGLKRDRYVKAIITAKNNVVSEPETGVVNEEEDQPSSIVIVPIIIPFRRR
                     KSSQYPNPKHGTNGVKVSNGESFEDVTATGIGSTKLMAQMAGKSSKTGRIRMGGTTRF
                     MGARRQRGVSRSGIATGLYGVQKYRGKITPIITTNIESGVTDRPLEDTNLPPKDPTAS
                     IIPKRQRSKGKETTQHGGRGFRRKQPAQSNNGGNSGPRGQRNFFPRSRYTTRRTRH"
     gene            1722..2702
                     /locus_tag="PKV52_02520"
     CDS             1722..2702
                     /locus_tag="PKV52_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPD99120.1"
                     /translation="MAYSNNPNLPKARATAMKMLIRDRLPLQRVANKCGVHRSTIYRW
                     KQKWNCLNQNVQLTNDYRPSGAYAKPYPSSFRLNACLWRIATESAAPHTSPHAISQQV
                     IDLVLQLRYTLKRCAEVVWHHLANLLGIKISLSSVHRILRRHHEYRGSRKNRVRPDNP
                     RRPLVTAPGELVQTNTIHYVDISTKKRRYVYTVIDLHTRMAYAEVHSRILPGKAAEVI
                     ANAQNLFGFSFSMVQADNGPEFSRYFEQVLNSKSIQVRHSRLGRPNDNANIERFNRTI
                     QEECLGNYIDHKVSNVKIQAKINQYLDYYNHERVHLGIQLRTPIQMLQRS"
BASE COUNT          732 a          630 c          533 g          882 t
ORIGIN      
        1 tcctatatac tattcagttt cacgtgtgat acttaatgac gtgttcttct tgtggtataa
       61 cgtgaccgtg ggaagaaatt cctttggcct cttgggccac tgttgccacc attattggat
      121 tgggctggtt gtttacgcct aaaacctcgt ccgccatgtt gagtagtttc cttacctttg
      181 cttcgctgtc ttttaggtat aatgctagct gttgggtctt ttggtggtaa gttcgtatcc
      241 tctagcggcc tgtctgtaac accagactct atatttgttg ttatgattgg tgtgatttta
      301 ccccgatatt tttgtacacc atataggcca gtagcaatac cgctacgtga cacgcctctc
      361 tgtcgtcttg cgcccatgaa tctagtagta ccacccatgc gaattctgcc tgtttttgac
      421 gatttgccag ccatttgtgc cataagttta gtactgccaa tgcccgtcgc tgtaacatct
      481 tcaaatgatt ctccattaga cacttttact ccgtttgtac catgctttgg atttgggtat
      541 tgcgatgatt tccttcttct aaaaggaata attattggta ctatcacaat tgagctaggt
      601 tggtcttctt cttcatttac gacacccgtt tctggttcac tcactacatt attctttgct
      661 gttataattg cctttacata tctatctcgc ttcaagcctg ccagtgtttc tcttgcatcc
      721 ttgggcagct cttgtgggtt tctgttataa agcgagacta aatcaactgt acgtataccc
      781 atcgtatcag caagttgttc tatatctttg ttaagctgtt tatcttgcgt ttctataccc
      841 ttgggttttt gcataacatc aatcacttgt tcatattctt cagcactcag ttgttgcctc
      901 aatagagcag tgacaagttt ttctactgct ttggcacgag ttagggcaag tgcttcgttt
      961 ttagcactgt ctattcctag accgggattt tcaccaccat acatatatgc atagtcatct
     1021 tcgtctgatg cgctaccgat aaaacgaatg tcagttattt cactatcatt ctgtataaga
     1081 tcagttattg tgtctgcaat tttttgcact tgtgccatgg ctactggttt ttctgccatt
     1141 gcatctttgt caaaaagacg agcccactcc ataagcgcta cccttagctg ttccttgact
     1201 atttcttggc ttgattctgg cgtacctgac ttagtgtctt tagcatcatc tggttgctct
     1261 gcaatctcta caccgggcaa cctcagtgtc gcatctccgg gtaagatatc aggcaaacct
     1321 ataccaatac tcgcttcatg gcttcttgtt tgcatcgttg caacagtagt aaaaccagtg
     1381 tgttctggtg cactattgtt ctgttgtgta gcacccatac tggcaagtac agataaagcc
     1441 acagagccaa accagcgacc tatgcgccat cgtctttgat tattatcttg ttctgtagta
     1501 gatacctgtt ctattgtgtg tgataaggga gttccttctc catatgttga cattatttta
     1561 tttccttttt gatatgtttt tgtttgtaat tgtatgatta tacattagca cagttttgct
     1621 tatttgtcaa ttataacgta atccttcacc acctttgcaa cataaagtag gcccccgtaa
     1681 actgtagtta cgatcaaata attacagaaa ggaggcctac tatggcctat tctaacaacc
     1741 ctaacctacc taaggcgaga gctacggcta tgaaaatgct cattcgcgac cgactaccac
     1801 tacaacgagt cgcaaataag tgtggcgtac atagaagtac tatctatcgc tggaagcaga
     1861 aatggaattg cttaaatcaa aacgtacaac ttacaaacga ctatcgtcct agtggcgcct
     1921 atgccaagcc ttatcccagt agctttagac taaatgcttg cttatggcgt atagccacag
     1981 aaagtgcagc tccacataca tcgccgcacg caattagcca acaagtcatt gatcttgtgc
     2041 tacagcttcg ctatacgctt aagcgctgtg cagaagttgt atggcatcat ctagctaatc
     2101 tattaggtat taaaataagt cttagcagcg tgcaccgtat attacgtaga caccatgaat
     2161 ataggggttc aagaaagaat cgagtgaggc cagataaccc acgccgtcca ctggttacag
     2221 caccaggcga actggtgcaa actaacacca tccactatgt cgacatatct accaagaaaa
     2281 gacgctatgt ctatacagtc atagatctac atactagaat ggcttatgct gaagtacata
     2341 gcagaattct acccggcaaa gcagctgaag taatagctaa tgcccagaac ctatttggct
     2401 ttagcttctc tatggtacaa gctgacaatg gcccagagtt tagtaggtac tttgagcaag
     2461 tgctaaacag caaaagcatc caagttagac atagtaggct aggcagaccc aatgacaatg
     2521 ctaacataga acgattcaac agaaccatac aggaagaatg cttgggtaac tatatagacc
     2581 acaaggtatc taacgttaaa atacaagcaa agataaacca atatctagat tactataacc
     2641 atgagcgtgt acacttaggt atacagttac gcacgcctat tcaaatgttg caaaggtcgt
     2701 gacaggtata cgaccccgta agctgtaatt aaatacccaa aatctgcctc tttttggcgt
     2761 caaattcttc ttgcgta
//
LOCUS       DAOPLS010000089         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_41556_length_2771_cov_3.427099, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000089 DAOPLS010000000
VERSION     DAOPLS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41556_length_2771_cov_3.427099"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PKV52_02525"
     CDS             complement(<1..847)
                     /locus_tag="PKV52_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPD99121.1"
                     /translation="MDITLNSIIKELIYLAVAFIIALIVRRVIHIFIEQYFARLYKSA
                     QHRGRSINKKRLNTLARAFKSLAAVSVWIIFIFVLLGHFNVNTAALLTGAGAVGIFFS
                     IAGKDIIMDLYVGFMALVEDQYRVGDVITIDQDHSGTVQEITLRTIKLRDIDGNIHIV
                     PHSLARSIINKTYDYSVVNVELGVAYDADMDAIKDIVNKTGEQIASEEDWKQIFIEPV
                     QYQTMLRFDESQQTFRAQGKVKPGKQWAAASEFRMRIKDAFDDNGIEIPFPQRVVRTV
                     TESAPK"
     gene            917..>2771
                     /locus_tag="PKV52_02530"
     CDS             917..>2771
                     /locus_tag="PKV52_02530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPD99122.1"
                     /translation="MSNPKTPTLNAAQQQAVDTTEGPVMVIAGPGTGKTQLLASRVLH
                     ILQNNPSLSPANILCLTFTEAGQIAMQKRLIELMGEAGAHVAVHTFHSFGSEIINTHP
                     DYFFNNVQFTPADDLSIHEVITSVLDALPARHPLTAKHEGDYALIGSIKRRIQECKKA
                     LTPDELAGVISDGDAFVRYIEPAIIDTFGTNVFTKKQFPQVAELLEVGLAYNNSSKII
                     TGIKPLHVVFNESLAAAYGTAIEEDSTKPLTVWKKDWFAKDVSKKPICKQRVVLEKLA
                     AFAEVYSNYQHELHKRRLFDFDDMIMQAVNSIETRGDLAYELQEQYQYLLVDEFQDTN
                     VVQMRLLRALADAPVHEGLPNVMVVGDDDQAVFAFQGAELSNILNFTETYPSAMQITL
                     TENYRSSHNILQAARAVITQGEDRLENYVKGIDKTLLPNATFATAPRSERWLFDIQAH
                     EYEYVAKHIAAQIKQGTVASDIAVIAREHKQLEALLPYLFDTDVPVAYERRENALNNP
                     QLKELLVLAKVVYYLSIDKPQVANELMPELLSADYWQLPATEIWQISLASRQIKTEYE
                     ANKLWLQIMLTGTVKQKNIARFLLEAAKLSINSSLEEILDVLIGSTDAFVAD"
BASE COUNT          896 a          603 c          536 g          736 t
ORIGIN      
        1 ttttgggtgc cgattctgtt acggtgcgta caacacgttg tgggaaaggt atttctatac
       61 cattgtcatc aaacgcatct ttaattctca ttctaaattc gctggctgca gcccactgtt
      121 taccaggttt aactttacct tgtgcccgaa atgtctgttg tgattcatca aaacgaagca
      181 tcgtttgata ctgcactggc tctatgaata tttgcttcca atcttcttcg ctggctattt
      241 gttcacctgt cttattaaca atatctttaa tggcatccat atcagcatca taagcaacac
      301 ccagttcgac attcacaaca gaataatcat aggttttatt aataatagag cgcgccaaac
      361 tatggggcac aatatgaata ttgccatcaa tatcacgtag tttaatggtg cgcaaggtta
      421 tttcctgaac cgtaccgctg tgatcttgat ctatggtaat aacatccccg acacggtatt
      481 gatcttctac aagtgccata aaaccaacgt acaaatccat aattatatct ttgccggcta
      541 tgctaaaaaa gataccaaca gcaccagcac cagtgagtaa tgcggcagta ttaacattaa
      601 agtgtcctag cagtacaaat ataaatatta tccaaacgct taccgccgct aaactcttaa
      661 aggctcgggc aagggtatta agccgtttct tatttatact tctacccctg tgttgggcag
      721 atttatacaa gcgtgcaaaa tattgttcta taaaaatatg tatgacacgc cgaacaatca
      781 gggcaataat gaaagctacc gctaggtaaa ttaactcttt gataatactg tttagtgtta
      841 tgtccatgct tatgattata gggcattatg ctaacttatc tgtcataaat tgtaaaactg
      901 tataaaatat agctatatga gtaacccaaa aactcctaca ttaaatgccg cccaacaaca
      961 agccgtagac acaactgaag gccccgttat ggttatagct ggacccggca caggtaaaac
     1021 tcagctgctt gcaagtcggg tgctccatat tctacaaaat aaccctagct tgtcaccggc
     1081 caacatatta tgcctaacgt ttacagaagc tggccaaata gccatgcaaa aacggcttat
     1141 agaactgatg ggtgaagctg gtgcccatgt tgctgtacac acattccaca gctttggctc
     1201 agaaataatt aatactcatc cagattactt ttttaacaat gttcagttta ctcccgccga
     1261 cgatctgagc atacacgaag ttattaccag cgttttagat gcgttacccg cacggcaccc
     1321 actcaccgca aaacacgaag gagactatgc attaataggc tctataaaaa gacgcataca
     1381 agaatgcaaa aaagcattaa caccagatga acttgcaggc gttattagcg atggcgatgc
     1441 gtttgttcgt tacatagaac cagctattat tgatactttt ggaacaaatg tttttacaaa
     1501 aaaacagttt cctcaggttg cagagctgct agaagtaggg cttgcataca acaattcatc
     1561 aaagattatc acgggtataa agccgcttca cgtcgtattt aacgaatcac ttgcggcggc
     1621 ctatggaacc gccattgaag aagattcaac caaaccactg actgtttgga aaaaagattg
     1681 gtttgcaaaa gacgtatcta aaaaacccat atgcaaacag cgtgtagtat tagaaaaatt
     1741 agcagcattt gctgaagtct atagcaacta ccaacacgaa ctacataaac gccgcttatt
     1801 tgattttgat gacatgatta tgcaagcagt caatagcatc gaaacccgtg gagatttagc
     1861 ctatgaactg caagaacaat atcagtattt gctagttgat gagttccaag acacgaatgt
     1921 tgtgcaaatg cgtttgctgc gcgcccttgc agatgcaccg gtacacgaag gcttgccaaa
     1981 cgttatggta gtaggggacg atgatcaagc cgtatttgca tttcaaggtg cagaactatc
     2041 taatatcctt aattttacag aaacatatcc cagcgcaatg caaataacac tcactgaaaa
     2101 ctacagaagt agccacaata tcttgcaggc agcacgagct gtaattaccc agggcgaaga
     2161 tagattagaa aattacgtga agggcataga taaaacacta ctacctaatg ctacctttgc
     2221 tactgcgcct cgcagcgagc gttggctatt tgacattcaa gcccacgaat acgaatacgt
     2281 cgcgaaacat attgcagcgc agattaaaca aggtacggtg gcaagcgata ttgccgtcat
     2341 tgcacgtgaa cacaaacaac tcgaggcgct cttgccatac ttatttgata ccgatgtacc
     2401 agtagcatac gaacgccgcg aaaatgccct aaataatcct caacttaaag aattactcgt
     2461 tcttgcgaaa gtagtctact acctaagtat agacaaacca caggtagcga acgagctgat
     2521 gccagaactc ctaagtgccg attattggca attaccagcg accgagatat ggcagataag
     2581 ccttgcaagc aggcaaataa agacggagta cgaagcaaac aaattatggt tacaaatcat
     2641 gctcacaggc acagtaaaac aaaaaaatat tgcaagattt ttgctagaag ctgccaaatt
     2701 gtctattaat agtagtttag aggaaatttt agatgtgctc ataggtagca cagatgcgtt
     2761 cgtggctgac a
//
LOCUS       DAOPLS010000090         2746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_42123_length_2746_cov_3.568933, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000090 DAOPLS010000000
VERSION     DAOPLS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42123_length_2746_cov_3.568933"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKV52_02535"
     CDS             complement(<1..343)
                     /locus_tag="PKV52_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99123.1"
                     /translation="MIQKIINILFKKRHFWRNVGFDELSELYTSEFLRSLAMSLIGIF
                     VPIYLYKLGYSLTDIFGMQVIWAAIRPPLSYMAAKSVAIFGPKHTMVLASIVQIVYLA
                     TLMSMQSAQWPL"
     gene            403..1683
                     /locus_tag="PKV52_02540"
     CDS             403..1683
                     /locus_tag="PKV52_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPD99124.1"
                     /translation="MHIFFSGIGGTGIGPLALIAKQAGYEVSGSDKQASQYTTYLQKH
                     GIQLYIEQTKEAIAKMHQTKPIDWFVYTSALPLENPHHPELMYVHELGIRHSKRDDFL
                     QYFLTEKKLKLIAFAGTHGKTSSTAMAVWTAKQLGVPISYSVGAKISFGDMGHYDPSS
                     KYFLYECDEFDKNFLAYQPYISVLTKVDWDHHEQYPTRKNYIDAFRQFVSQSKNVIAH
                     KSELKYLSIESKNNVHEIDESLLPLLKIKGAHNRSNAAGVATAMAMIQRTNDYESIIR
                     ALNAYPGSNRRFEKLTDNIYSDYAHTPEEIAATLQLAKEYGKSIVVVYEPLTNRRQHF
                     MRDAYKDVFQQLDALYWLPSYLAREDPELDIIHPEQFIAQLPESAHAHTAKLNDELKS
                     TIIQASNSGALVLLLAGGGGGSLDEWARKAFVKR"
     gene            complement(1680..1979)
                     /locus_tag="PKV52_02545"
     CDS             complement(1680..1979)
                     /locus_tag="PKV52_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99125.1"
                     /translation="MKLIFRHPKPKELNLAWMIGLTIAVSAVLVFISFFVFLNSGAYA
                     TVKQISAAQQVLQSDLDDIDTTSPIQADDLEYYSRTLPQRVKSFNDAADFGPTGL"
     gene            complement(1981..2538)
                     /locus_tag="PKV52_02550"
     CDS             complement(1981..2538)
                     /locus_tag="PKV52_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99126.1"
                     /translation="MRLLVISVLLLVMGVAPNVLAEGQIDSKDRLMELQDKRSVSILP
                     QDRAVVSEQCINNKQTIKTLQTATDESAKKRIATYGAIQKEIKAIELRMSKQGADASE
                     VDLLIGKLQQSLDTFTEKSRYSQQLAEDITAVDCVNSPELYKAGIAEYNQTRQEMFTL
                     ASELKVTVLLSPQTTFSPLINRLRI"
     gene            complement(2557..>2746)
                     /locus_tag="PKV52_02555"
     CDS             complement(2557..>2746)
                     /locus_tag="PKV52_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99127.1"
                     /translation="FIGSRRQVRGSVILLLRQTVYLTRDELHRAIDDDRLDSVLADLI
                     KEGLITSNNTGSYRLASG"
BASE COUNT          864 a          542 c          527 g          813 t
ORIGIN      
        1 cgagcggcca ctgagcactt tgcatactca taagagtagc taggtataca atttgcacaa
       61 tagaagcaag caccattgtg tgctttgggc caaatatagc aacactttta gccgccatgt
      121 aagatagcgg tggccgtata gctgcccata ttacttgcat tccaaaaata tcagtcagtg
      181 aataaccaag tttgtataga tatattggca caaaaatacc aattaaactc atagctaagc
      241 tacgcaaaaa ttcactcgtg tacagttcac ttagttcgtc aaaaccgacg ttacgccaaa
      301 aatgacgttt tttaaatagt atgtttataa ttttttgtat catgaggtaa aaataccatg
      361 cactactgta agtatagcaa tttataaggt atgatgacaa ttatgcatat atttttttct
      421 ggcataggcg gtactggtat tgggcctctg gcattgattg ccaaacaagc tggctatgaa
      481 gtatctggat ctgataagca agcaagccaa tatacaacgt atttacaaaa gcacggcatt
      541 cagctttata ttgagcaaac aaaagaagca attgcaaaga tgcatcaaac aaagcctata
      601 gactggtttg tgtatacgtc tgcgttaccc ttagaaaacc cccaccatcc cgaattaatg
      661 tatgttcatg agttaggtat tcgtcactct aaaagagatg attttttaca gtacttttta
      721 acagaaaaaa aactaaaact aattgctttt gcaggtacac acggaaaaac cagttcaact
      781 gcaatggccg tatggacagc taaacaactc ggtgtaccaa taagctattc agttggcgca
      841 aaaattagtt ttggagacat gggccattac gacccatcga gcaagtattt tttgtatgaa
      901 tgcgatgaat ttgataagaa ttttttagct tatcagccat acatcagtgt actcacaaaa
      961 gtagactggg atcatcatga gcaataccct actagaaaaa attatattga tgcttttagg
     1021 caattcgtga gccaatctaa aaatgtaatt gcgcataaaa gtgaattaaa gtatttatca
     1081 atagaatcaa aaaataatgt tcatgaaata gatgaatcac tactaccgtt gctaaaaata
     1141 aaaggtgccc acaataggag caatgcagca ggtgtggcga cagctatggc aatgattcag
     1201 cgtacaaacg attatgaatc aattatacgc gccttaaacg cctatcctgg gtctaatagg
     1261 cgctttgaaa aactaaccga taatatatac agtgattacg cccacacacc agaagaaata
     1321 gctgcaacgc tacagcttgc gaaagagtat ggcaaatcaa tcgttgtggt gtacgagcct
     1381 ttaaccaaca gacgccaaca ttttatgaga gatgcctata aggatgtttt tcaacagtta
     1441 gatgcacttt attggttacc cagttatctt gcgcgcgaag acccagaact agatattatt
     1501 cacccagaac aattcattgc tcaattacct gagtctgcac atgcacacac tgccaagctc
     1561 aatgacgaac taaaatctac tatcatacaa gcttctaaca gcggtgcgct agtgttacta
     1621 ctcgcaggag gaggtggtgg tagcttggat gaatgggctc gtaaagcgtt tgttaagcgc
     1681 taaagaccag tcggtccaaa gtctgcagca tcattaaatg acttaacccg ttgaggtaat
     1741 gttcttgaat aatactctag atcatcagcc tgaatcgggc ttgtagtatc aatatcgtct
     1801 aagtcgcttt gcagaacctg ttgggctgca cttatctgct taacggttgc ataggctcca
     1861 ctatttaaaa aaacaaaaaa agatataaac actaaaactg cagacacggc gattgtaaga
     1921 cctatcatcc aggctaagtt gagttccttt ggtttagggt gtctaaaaat tagtttcatt
     1981 ttatattctt agtctattta tcagtggact aaatgtcgtt tggggcgata gcaatacggt
     2041 tacttttaac tcacttgcca aagtaaacat ttcttgccgt gtctgattat attctgcgat
     2101 accagcctta tatagttctg ggctgttgac gcaatctact gctgtaatgt cttctgctag
     2161 ttgttgagaa tagcgtgact tttcggtaaa cgtatctaaa ctttgttgta atttgccaat
     2221 caacaaatca acttctgatg cgtctgcgcc ttgtttggac atacgcagtt ctattgcttt
     2281 aatctctttc tgaatagcac cgtaagtggc aattcgcttt ttagcagatt catctgttgc
     2341 tgtctgcaaa gtcttgatag tttgtttatt gttaatacat tgttctgata ccaccgcgcg
     2401 gtcttggggt aaaattgaaa cacttcgctt atcttgcagt tccattagtc tatctttaga
     2461 atcaatttgg ccttctgcaa gtacattagg agcaacgccc attacgagga gtagcacaga
     2521 tataaccaat aatctcatta gtaatagtat atcattttac ccactcgcaa gacgataaga
     2581 acctgtattg ttactcgtta tgagtccttc ttttataaga tcggcgagga cactgtctaa
     2641 acgatcatcg tctatagcgc gatgtaattc atcacgggta agataaactg tttgacgtaa
     2701 cagcagtatt acgctacctc ttacctggcg tctagaccca ataaag
//
LOCUS       DAOPLS010000091         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_42386_length_2734_cov_3.472564, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000091 DAOPLS010000000
VERSION     DAOPLS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42386_length_2734_cov_3.472564"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..248
                     /locus_tag="PKV52_02560"
     tRNA            174..248
                     /locus_tag="PKV52_02560"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Met,seq:cat)
     gene            283..486
                     /locus_tag="PKV52_02565"
     CDS             283..486
                     /locus_tag="PKV52_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99128.1"
                     /translation="MYGVQDEILSAIAELVQIIQAISAELYDETEFIIPVFKINPARS
                     YIPAPATKKKSLAFARDFFFILG"
     gene            complement(666..1208)
                     /locus_tag="PKV52_02570"
     CDS             complement(666..1208)
                     /locus_tag="PKV52_02570"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD99129.1"
                     /translation="MKAPELKTTDSLITLIVPDVQRDTKLGVQWLNGELGHVTMQSMG
                     NTKDVIDNILPTTVETESERVRNFLERTDQLNWMIEYNGTVVGSIWVDLEDSHYLPGP
                     SVHIMVGDPAMRGKGIGSASVSAVIEYLQGLGSKVIYSRHLITNEASSRLLRQIGFVN
                     VGEPYTSDTLKFQNLVLEIS"
     gene            complement(1255..1812)
                     /locus_tag="PKV52_02575"
     CDS             complement(1255..1812)
                     /locus_tag="PKV52_02575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPD99130.1"
                     /translation="MAHIQVIVGSTRPGRVGRNVADWFMSQAQIPAGSTVEIIDLADI
                     NLPMLDEPKSAMMDQYSKDHTKSWSQIISKADGYVWVTPEYNHGTSAVLKNAIDYLYK
                     EWAYKPVAFVGYGGMGATRAIEHLVNIASELRMFPLKSRYHVLDVWAAFNNDGSIKPE
                     LVRGDASSLLSEVTKVSDATKSLRQ"
     gene            1921..2247
                     /locus_tag="PKV52_02580"
     CDS             1921..2247
                     /locus_tag="PKV52_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD99131.1"
                     /translation="MKTQSAPQELQKAGCIAKALQILGDKWTPLLIRELTLCPQTFSD
                     LEKLLIGISPRTLSQRLNMLVQEDIVDKRLYCEHPPRYSYQLSPKGRDLQTILQDMAQ
                     WSAKYK"
     gene            complement(2248..>2734)
                     /locus_tag="PKV52_02585"
     CDS             complement(2248..>2734)
                     /locus_tag="PKV52_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPD99132.1"
                     /translation="TLLISKTEMSKVPARWNDIAHIRTIRDSVAPHTLVVGNGDVLSR
                     QEGEQLAMQHRLDGIMIGRAIFHDPYVFATQSPWAYTSQVKKLQMYKQHIELFCQTYE
                     PKNDETRWVRNWESLKRMAKIYIHGIEGASAFRDVFMRSTTAAEMLQHLDNEITKLQN
                     N"
BASE COUNT          737 a          610 c          556 g          831 t
ORIGIN      
        1 tactattgca ggttcgagca gtggcagcat cgcgaaggct cataatcgcg atcagcgtaa
       61 gcggcgaata tatgataagc cagagaaaat ctaccattta caattaacta ttttcacgga
      121 gcgaagctat ataaccacaa ggttatcccc ttcacttctt aaccacccta ttttgcgggg
      181 tagagcagtg gcagctcgtg tggctcataa ccacaaggtc gcaggttcga gtcctgcccc
      241 cgcgaccaag aaaaatcact caccttgtgt gggtgttttt tcttgtacgg agtgcaggac
      301 gagatccttt cggcaatagc cgaactggtt cagataattc aagcgataag cgcagaatta
      361 tatgatgaaa ccgaatttat cattccagta tttaagataa atccggctag aagctatatt
      421 cctgcccccg cgaccaagaa aaaatcttta gcatttgcta gggatttttt ctttatcttg
      481 ggataagact cggttctgcg actcggcaag cttacttgac gatagtcgca ggttggaaga
      541 caaagcttgc gcgcaggtgc ttgcaccgag cacaagctgc aggcgcatcg agccttagca
      601 agatattcct gcccccgcga ccatgtaaaa gagttaggta cgagttaaaa ggtttagata
      661 atttactacg atatttctag tactagattt tgaaatttta gtgtgtcgct cgtatatggc
      721 tcgcctacgt taacaaatcc tatttgtctg agtaatctac tactagcttc gtttgtgatt
      781 agatgtcttg aatatatgac cttactgcca agcccttgaa gatactcaat aacggcacta
      841 acactagcag aaccaatacc tttaccccgc atagctgggt caccaaccat aatatgcact
      901 gatggaccag gtaggtaatg tgagtcttct aagtcaaccc aaatagaacc aactactgtg
      961 ccgttatatt caatcatcca gttcagctgg tctgtacgtt ctagaaagtt tctgaccctt
     1021 tctgattctg tttctactgt tgttgggagt atgttatcta taacgtcctt tgtgttcccc
     1081 atagattgca tagtgacatg tcctagttca ccattaagcc attgcacgcc aagtttggtg
     1141 tcacgctgta cgtctggaac tattagtgtt ataagggaat cagtagtttt aagctcgggt
     1201 gccttcatca ctgtatttta gcaggtattg tctgtggcgg tctttcttca agttttattg
     1261 ccgtaaactc tttgtagcgt ctgaaacttt ggttacttct gacagcaacg agctggcatc
     1321 accacgcacc agctctggtt taatagaacc atcgttatta aaggcagccc atacatcaag
     1381 cacgtggtac cgtgatttta gcgggaacat acgtagttcg ctggcaatat ttactaaatg
     1441 ctcaattgca cgggtcgcac ccatgccacc gtagccgaca aaggcaacag gtttatatgc
     1501 ccattcttta tataaataat ctatagcatt tttaagtacc gcagatgtac cgtggttata
     1561 ttctggggtt acccacacat aaccatctgc tttgctaatg atttgtgacc atgattttgt
     1621 atgatcttta ctgtattgat ccatcatcgc acttttaggt tcatctagca ttggcaaatt
     1681 aatatctgct aaatctataa tttcaaccgt tgaaccagcc ggtatttggg cctgagacat
     1741 aaaccaatcg gcaacgttac ggccaacacg tccagggcgg gtagagccaa ctattacttg
     1801 tatatgcgcc atatttgtat ctccttttct gtatatagta tatacgcttt actttgtata
     1861 gtcactacta tactgcatat cactatactt tgtaaagtct atttatggta tactttttgt
     1921 atgaagactc aatcagcacc ccaagaacta caaaaagctg gttgcattgc aaaagcactg
     1981 cagatacttg gtgataaatg gacgcccctg ctgattcgtg aactaacgtt atgcccgcag
     2041 acatttagcg atttagaaaa attactaata ggcattagcc cgcgcacact aagccagcgt
     2101 cttaacatgc ttgtgcaaga agatattgta gacaagcgct tgtattgtga gcacccaccc
     2161 cgctattcat accagctgag cccaaaaggt agagatttgc aaaccatact tcaggacatg
     2221 gcccagtgga gtgcgaaata taagtaatta gttgttttgt agctttgtaa tctcgttatc
     2281 tagatgttgc aacatttctg ctgctgttgt gctgcgcata aagacatctc taaaagcgct
     2341 tgccccttca ataccatgaa tatatatctt tgccattcgt tttagacttt cccagttacg
     2401 gacccaccta gtttcatcgt tttttggttc ataagtttgg caaaataatt ctatgtgctg
     2461 tttgtacatt tgtagttttt ttacttgtga agtatatgcc cacggacttt gtgtagcgaa
     2521 tacatatggg tcatgaaaaa tagcccgccc aatcataatg ccatctaagc ggtgctgcat
     2581 tgctagttgt tcaccctcct gcctgctaag tacatcacca ttgccaacaa caagagtgtg
     2641 aggcgccacg ctatcccgta tcgttcgtat gtgcgcgata tcgttccaac gagctggtac
     2701 tttgctcatt tctgttttag aaatgagcaa agta
//
LOCUS       DAOPLS010000092         2727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_42524_length_2727_cov_4.263473, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000092 DAOPLS010000000
VERSION     DAOPLS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42524_length_2727_cov_4.263473"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..856)
                     /locus_tag="PKV52_02590"
     CDS             complement(365..856)
                     /locus_tag="PKV52_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HPD99133.1"
                     /translation="MTKHEWYLILSAAVALKSKDPSTKVGCIMVDAQRGDVLAQGYNG
                     FPRHWPDETIATMSRTDKYALTIHAEQNAIANAAARSAALNGCIAYVTHHPCSDCTKL
                     MIQAGVAEVHFVTPQPELRQRWIDSFDLSIKMFSTVGIIHHEHKLDINELLSKAAAGA
                     HTP"
     gene            complement(907..1374)
                     /gene="smpB"
                     /locus_tag="PKV52_02595"
     CDS             complement(907..1374)
                     /gene="smpB"
                     /locus_tag="PKV52_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPD99134.1"
                     /translation="MKQKKPTRSTITNKKARFDYDIKTEYLAGIVLNGPETKSLRFSR
                     GSLRGAFVTIKNGEAWLNNLQIMPMNTNAAHLTEDQQTRARKLLLKRRELADIIASKD
                     QGNAIVPLALLTKGRYIKVHIATAKGKKAYDKRAKIKERDTHRDAKRDLKKYQ"
     gene            1443..2480
                     /locus_tag="PKV52_02600"
     CDS             1443..2480
                     /locus_tag="PKV52_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99135.1"
                     /translation="MKNTQPSVANSCYSQDYYPATGIYAPPTEISDEHTSAKLDEIPM
                     GTLLNVPHPTVVDLSDGSQIRLYNVTPRGDYDESRTIVYVPEFAAGMVPHRDAAALAI
                     AEVLGCRVIVAPHNELSTTRQERAQLRAGNVGFIAARLAEALAKVLPVGQDTTIYGYS
                     FGAQIAPDLSGEVSRHGVLDHKKLALLEPPNTVERSMYQLLKDFSKAGTEQWLQATRD
                     SRWQALIELVDSTYVHEDAGRFIRQARSLASLAMIGAMKRGTFAADLLAAHQALPPDA
                     RTTLGYVENSAMVAPFGLEDALDAVQASSNNPDAIRLVRFTSGSEAKGVGHAGGNNPL
                     VAISLLEKTTE"
     gene            complement(2470..>2727)
                     /locus_tag="PKV52_02605"
     CDS             complement(2470..>2727)
                     /locus_tag="PKV52_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99136.1"
                     /translation="KDSMLHELQAEPWTPTDVKTVSIEEQDKSMNADRLRERIQYGVA
                     TGFRDIDLWGGEWWYWRKQTQDDPSLWEAVREEKAKALSTQ"
BASE COUNT          699 a          600 c          632 g          796 t
ORIGIN      
        1 tctgtgactg gtactgtgct tcccagatca cgcactgctt actcttagca agcaataagc
       61 actgatgcga tctgggaaat gttcaacagt atttttagat taaactaacc gagtagccaa
      121 tgaccttcgt tcatttgtag aagcgtctat cttgtacctg gtttagtttc tctttttggt
      181 tcctgactcg ctaactgagc accttagtgt actgcaaggt cggtgcgcac tatatgagcc
      241 aggaatatca ctagaaaaga gcctgatatt tttgtttaat cgctacttta attaaatagc
      301 gtgagaacca ttatagtaca aaatactata aatgtcaata gatatactca tttagcaggc
      361 atcgttaggg tgtatgtgca ccggcggcag cttttgaaag aagttcgttt atgtctagct
      421 tatgctcatg atgaataatg cccactgtac tgaacatttt aatagataaa tcaaaagaat
      481 ctatccatcg ttggcgtaat tcgggctgcg gtgtgacaaa atgaacttcg gcgactcctg
      541 cttgaatcat cagtttagtg cagtcagaac acgggtgatg agtaacgtaa gcaatgcagc
      601 cattcagtgc tgcgctgcgc gcggcagcgt tagcaatagc gttttgttca gcatgtatag
      661 tcagtgcgta tttgtctgta cgtgacatag tggcaatagt ttcgtctggc cagtggcgtg
      721 gaaaaccgtt ataaccctgt gctagtacat cccctcgctg ggcatctacc ataatacagc
      781 ctactttcgt agagggatct ttgcttttga gtgcaactgc cgcgcttagg attaaatacc
      841 attcatgttt tgtcacacgt actgtattct tatgtttata cagctattgt agcgcttttc
      901 ggctatttat tggtactttt taagatctcg ttttgcgtcg cggtgagtat cacgctcttt
      961 tattttggca cgcttgtcgt aagctttttt gcccttggcg gtggcaatgt gcaccttaat
     1021 gtagcggcct ttggtgagga gtgccaatgg cacaatggca ttcccctgat cttttgaggc
     1081 aataatatcg gctagttcac gtctttttaa cagtagtttg cgggctctgg tttgttgatc
     1141 ttcggtcaaa tgtgcggcat tagtgttcat gggcataatt tgtaaattat tgagccatgc
     1201 ttcgccattt ttaatggtta caaaagctcc ccgcaaactc cccctgctaa agcggaggct
     1261 tttagtttct gggccattaa gcacaatacc agcaaggtat tcggttttaa tatcataatc
     1321 aaaccgcgct ttcttatttg taattgtact tcgtgtgggc tttttttgct tcataccgta
     1381 tgtattgtag caatcattcc gcttattgta aaaataatat aattgtgcta taataagtca
     1441 ccatgaaaaa cactcaaccc agcgtagcga atagctgcta ctcacaagat tattatccag
     1501 caaccggtat atatgcacca ccaacagaaa ttagtgacga acataccagc gcgaaactag
     1561 atgaaattcc catgggaaca ttactaaatg tgccacaccc tactgtggtg gatctatctg
     1621 atggttcaca aattcgcctt tataatgtta cgccaagggg tgactatgac gaatcacgca
     1681 caattgtgta tgtgccggaa tttgcagccg gcatggtgcc acatcgtgac gcagcagcgc
     1741 tcgctatagc agaagttttg gggtgccgtg ttattgtagc cccacataac gaactgagta
     1801 caacgcggca agagcgggca cagctccgtg ctggaaacgt aggtttcatt gcagcccgcc
     1861 tggcagaagc tctagctaag gtgttgccag taggccaaga cacaacaatt tatgggtata
     1921 gctttggtgc acaaattgca cctgacttgt caggagaagt aagccgtcat ggggtgctag
     1981 atcacaaaaa gctcgcacta ctagagccac caaacacggt tgaacgcagc atgtaccaat
     2041 tactaaaaga tttttcgaag gccggtactg aacagtggtt gcaggcgacg cgagattcgc
     2101 gctggcaagc cctgattgaa ctcgtagaca gtacgtacgt gcacgaagat gcgggacggt
     2161 ttattagaca agcacgtagc ttagccagct tagcgatgat tggcgcaatg aaacgcggca
     2221 catttgctgc agatttattg gctgcgcacc aggcgctgcc gccagatgca cggactaccc
     2281 ttggctacgt agaaaatagc gcgatggtag caccatttgg gcttgaagac gcgctagacg
     2341 ctgtacaagc cagcagtaat aatcctgatg ctattcgcct tgtaagattt acatctggat
     2401 cagaggcaaa aggcgttggt catgctggtg ggaacaaccc attggtcgcg atatctttac
     2461 ttgaaaaaac tactgagtag ataatgcttt ggctttttct tcacggacag cttcccaaag
     2521 acttgggtcg tcttgggttt gcttgcgcca ataccaccac tccccacccc ataaatcgat
     2581 atctctaaaa ccagtcgcta caccatattg tatgcgttca cgtaaccgat cggcattcat
     2641 tgatttgtct tgttcttcta tacttactgt tttaacgtcg gtgggtgtcc agggttcggc
     2701 ttggagttcg tggagcattg aatcttt
//
LOCUS       DAOPLS010000093         2721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_42663_length_2721_cov_5.034884, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000093 DAOPLS010000000
VERSION     DAOPLS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42663_length_2721_cov_5.034884"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..551
                     /gene="rnpB"
                     /locus_tag="PKV52_02610"
     ncRNA           190..551
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKV52_02610"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(612..956)
                     /gene="rplT"
                     /locus_tag="PKV52_02615"
     CDS             complement(612..956)
                     /gene="rplT"
                     /locus_tag="PKV52_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPD99137.1"
                     /translation="MRVKRGATSHKKHLKVRAATKGMTHSRRRSYRMGKQGVIKAMQY
                     SYRDRRNKKRTFRTLWNARINAAARLNNTTYSAFISSLKKTNITLDRKILSELAVNEP
                     TAFADIVKKASK"
     gene            complement(959..1153)
                     /locus_tag="PKV52_02620"
     CDS             complement(959..1153)
                     /locus_tag="PKV52_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HPD99138.1"
                     /translation="MPKLKSHSGTKSRVKITKTGKVMSRKSFGNHFLEKKSASRKRTY
                     AGMREMTGKTRTNIKRKLGV"
     gene            complement(1131..1658)
                     /gene="infC"
                     /locus_tag="PKV52_02625"
     CDS             complement(1131..1658)
                     /gene="infC"
                     /locus_tag="PKV52_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPD99139.1"
                     /translation="MNKHTRLNEAIRATELRVIDEDGNQLGVLSKQEALRAAQAAELD
                     LVEISPGATPPVAKIIDWGKYNYQKTKQLQKSRKNAKAGDLKQMRFGLKISDHDIAVK
                     LKKVVSFLEDGDKVRISVIFRGRELAHKEIGYALMDKIINIIGEIAVVDQPPQFAGRQ
                     ISAVIRSSHAKVKKP"
     gene            complement(1724..>2721)
                     /locus_tag="PKV52_02630"
     CDS             complement(1724..>2721)
                     /locus_tag="PKV52_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD99140.1"
                     /translation="PLIAAQPGLLSTNKISALVEPIDFAEIIGQQAAKRALIIAAAGG
                     HNILLSGPPGTGKSMLAKAFISILPDLTLEQSLETTHIHSLAGAEIEMLLTHPPLRSP
                     HHTSSNVAIIGGGHTLRPGEISLAHNGVLFLDELPEFPRSIIEALRQPLEDNVVTISR
                     AQSSTTYPSDFILMATSNPCPCGYYGSTKACTCNAHEIARYQKKISGPILDRIDMHFT
                     VSTVDHENLLKKTSLKESPDIKVIVSTARKIQKQRTDAPNARLSNKELKRVMNINKDA
                     ESFLNSAAQKMDISARSYMKTVKLARTIADLEKSNEITTAHITEALQYRPKQTVL"
BASE COUNT          700 a          558 c          589 g          874 t
ORIGIN      
        1 atatacaaaa ttaaaaacag ggagttgtgg ccaatacatc aagcaattaa gcaattgtgt
       61 gaacattctt tcgatagagt gtcatttact tacataccac gtgaatacaa taaagaggct
      121 gatgcaatgg tgaacgaagc cttggatgcg ttcgcgaata gtaagtaaga tgtactataa
      181 tagatgtagc tagattgccg gtcggtcgcc tgcattcaaa aaaatgcggg aggaaagtcc
      241 gggcagtata gggcacaaca gttgctaacg gcaaccaaga gtaatcttag ggaaagtgcc
      301 acagaaacga taccgcctaa ctatgacagc aatgcatact aggtaagggt gaaatgagtg
      361 gggtaagagc ccacagctta ctatggtgac atagtaggag ggtaaaccct gttggctgca
      421 aggattgggt agtgcttttt aagctagtgg cccgctatac ccaagtcaac ccgcttgaga
      481 ttcgtagcaa tacgattcct agatagatga tcgacgtatc acagaacccg gcttataggc
      541 aatctggcta cacaaaaacc catcatttgt gatgggtttt ttaggtgaaa gctaggcctc
      601 agtatggtta attacttaga tgcttttttc actatatctg caaatgccgt aggttcgttg
      661 acagcaagct ctgataagat ttttctgtct aatgttatgt tagtcttttt taaagaacta
      721 ataaacgcgc tgtaggtagt gttattgagt cttgctgctg cattgattcg cgcattccat
      781 aacgtacgga acgtacgttt tttgttacgc ctatcacggt acgaatactg catggctttt
      841 ataacaccct gtttgcccat gcggtagcta cgacgtcgcg agtgcgtcat gccttttgtg
      901 gcggcacgta ccttaagatg ctttttatgt gaggttgcgc ctcgtttaac tctcattgtt
      961 agactcctaa ttttcgctta atatttgtac gagttttacc tgtcatttcg cgcataccgg
     1021 cgtaggttcg cttccgagat gctgatttct tctctaagaa gtggttccca aagctctttc
     1081 gggacatcac tttaccagtt ttggtaattt ttacccgact ctttgttcca ctatggcttt
     1141 ttaactttgg catgactact ccttatgact gcactaattt gtctaccagc gaattgaggt
     1201 ggttgatcaa caactgcaat ttcaccaata atgttaataa ttttatccat taacgcatac
     1261 ccaatttctt tgtgagccag ctcgcgacca cgaaagatga cactaatgcg tactttgtct
     1321 ccatcttcta gaaaagaaac aactttctta agcttaacag caatatcgtg atcgctaatt
     1381 ttaagcccaa agcgcatttg ttttagatcg cccgctttgg cgttcttcct acttttttgc
     1441 agctgtttcg tcttttgata attgtattta ccccaatcta ttatttttgc aactggtggt
     1501 gtcgcaccgg gggaaatctc taccaaatct agttctgctg cctgagctgc acgaagagcc
     1561 tcttgtttag acagtacccc taattggttt ccatcttcat ctataactcg taattcggtc
     1621 gctcttattg cctcgttgag acgggtgtgc ttgttaatgt aatcttctcc tttttcagat
     1681 actgactata acagattacg tcactggggt caacaattta gatttataat accgtttgtt
     1741 ttggtctata ttgcagcgct tcggtaatat gtgccgttgt tatctcattt gatttctcta
     1801 ggtcggcaat cgtacgggct aactttaccg tcttcatgta tgaacgtgcc gatatatcca
     1861 ttttttgagc ggcactattg agaaatgatt ctgcatcttt gttaatattc atcacacgct
     1921 taagttcttt attagatagc cgtgcgttcg gggcatctgt gcgttgtttt tgtatctttc
     1981 tggctgtgct aacaatcact tttatgtcag gagattcttt aaggctagtt ttctttagta
     2041 aattttcgtg atctacagta gacacagtaa aatgcatgtc gatacgatct aatattggtc
     2101 ctgaaatctt tttttggtat ctggctattt cgtgggcgtt gcaggtgcat gctttggtgc
     2161 tcccataata cccacacgga catggattgc tggttgccat aagtataaaa tcacttgggt
     2221 atgtggtact agattgcgcc cgagagattg ttacaacatt atcttctagt ggctgacgaa
     2281 gagcttctat aatagaccga gggaactcgg gtagctcatc taaaaacaaa accccattgt
     2341 gcgcaagact tatttcacca gggcgtagcg tgtgcccacc accaataatc gcaacattac
     2401 tagaagtatg atgtggtgac ctaagtggtg gatgagtcag tagcatttct atttctgctc
     2461 ctgccaagct atggatatgt gttgtttcta gtgattgttc caaggtaaga tcgggcaaaa
     2521 tacttatgaa tgctttggca agcatacttt ttcctgtgcc agggggccct gataacagaa
     2581 tattatgacc gcctgctgca gcgataatga gtgctcgttt tgcagcttgt tgcccaataa
     2641 tttcagcaaa atcaatgggt tcaacgagag cagaaatctt gttcgtagac agcaaaccag
     2701 gctgagctgc tattaaaggt c
//
LOCUS       DAOPLS010000094         2719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_42716_length_2719_cov_4.720721, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000094 DAOPLS010000000
VERSION     DAOPLS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42716_length_2719_cov_4.720721"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..491)
                     /locus_tag="PKV52_02635"
     CDS             complement(21..491)
                     /locus_tag="PKV52_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99141.1"
                     /translation="MQTNKKQHKTTNLDRSTVFKFGLYAVGAILLVQLFLVQIIRHDY
                     YRAEALAEHVKKFEIPAKRGVISLSDGYSATPVVLNEKKFLVYADPKFIQNTEAAAKQ
                     LQEVVGGNADDIKNLLTTTSRYVVVINPAKIKKIITKKVNKKREIKNFPSVFTN"
     gene            complement(538..885)
                     /locus_tag="PKV52_02640"
     CDS             complement(538..885)
                     /locus_tag="PKV52_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99142.1"
                     /translation="MTYGKSITLSRAAPYQRNRNVVSLKSNRTGFGPVANSVIIVLIL
                     CLLGLVYLTQVTKTNALGYKVSELSSQQEQLSEEHASLELDSVRLQNLERIKNSQVAQ
                     NLQQAQPTAYVTQ"
     gene            complement(1038..1901)
                     /gene="rsmH"
                     /locus_tag="PKV52_02645"
     CDS             complement(1038..1901)
                     /gene="rsmH"
                     /locus_tag="PKV52_02645"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPD99143.1"
                     /translation="MHEPVLLQEVIAAMNPTSGERYLDATAGYGGHAREILAHTHNFS
                     ESVLVDRDKAAVDHLMAEFATTRVKIVRNDFYSAAEDLVAAGQQFDLILADLGVSSLH
                     LDKASRGFSFAAIGPLDMRMDQRQTLTAETVVNEWSAESLEQILRNYGEEPKARRIAK
                     AIVAHRPIATTSELANIVARTTGRWTGKHPATRTFQAIRIAVNDELRLLERVMPLWVA
                     ILRPGGRLGIISFHSLEDRLVKRFFLEHGGDRFDASLSIVTKQPVVAGSQEIAINPRS
                     RSAKLRVAAKI"
     gene            complement(2235..2594)
                     /locus_tag="PKV52_02650"
     CDS             complement(2235..2594)
                     /locus_tag="PKV52_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99144.1"
                     /translation="MTTVDYFERKLDDKRRLTIPAELRSEFKSGVVVTKGFGTYLHMY
                     SEEVWNSQVEPELTGYILDEKVADLNVQFRSGKTSAELDQKQGRVTLEQHLLDFAGIT
                     KDVVAVRAGAYWRLSAK"
BASE COUNT          665 a          570 c          568 g          916 t
ORIGIN      
        1 catgggcacc attaaactct tcaattcgta aacactgacg ggaaattttt aatttcccgt
       61 tttttattta ccttttttgt aataattttt tttattttag cagggttaat tacaaccaca
      121 tacctactcg tagtcgtaag taagttcttt atatcgtctg cgttgccacc aacaacttct
      181 tgaagttgct tagcagctgc ttcagtgttc tggataaact taggatcggc gtacactaag
      241 aacttttttt cattaagcac cactggagta gcgctgtaac cgtctgataa gctaatcaca
      301 ccgcgcttgg caggtatttc aaactttttt acatgctctg caagtgcttc tgcacggtag
      361 taatcgtgac gaattatttg taccaaaaaa agttgcacta ataaaatagc gcctaccgca
      421 tataagccaa acttaaaaac cgtactacgg tctaagttag ttgttttatg ctgttttttg
      481 ttcgtttgca tcgtaaataa cagataaact gcttatagtt atattaccac tttttggtta
      541 ttgtgtcacg tatgcagttg gctgtgcttg ttgcagattt tgtgctactt ggctgttttt
      601 gatacgttca agattttgta gacgaacaga gtctagctca agactagcgt gttcttcaga
      661 caattgttct tgttggctag aaagttcgct caccttatag cctagtgcgt tcgttttggt
      721 aacttgcgtc aggtatacca agccaagcaa acataatatc agcacaataa ttacgctgtt
      781 agcaactggc ccaaaacctg ttcggttgga ctttaagcta acgacattgc gattgcgttg
      841 atatggtgca gctcgtgata gtgtaattga ttttccgtat gtcatatgtt tgttttttgg
      901 tgattgtgca actcttgttt gaccaagagt tgcacaatct atgcgtcgat aaagtgccta
      961 tagtacgtga acgtgtactt tttgggctac cgaccgacca gtgactttaa ttttcatcgc
     1021 tggtcctttc tatttgttta tatttttgcg gcgacacgga gttttgcact tcgtgaacgc
     1081 ggattaatag caatttcttg tgatccggct acaaccggtt gtttggtgac aatagaaagc
     1141 gaagcatcga agcgatctcc tccatgttcg agaaaaaagc gttttacaag acggtcttcg
     1201 agtgagtgaa aacttatgat acccaaccgc ccacctgggc gcaagatagc aacccatagt
     1261 ggcatcactc tctctaataa ccgtagctcg tcgttgaccg caattctaat tgcctgaaag
     1321 gtgcgagtgg caggatgttt acctgtccac cggccggttg tacgggccac aatattggca
     1381 agttctgacg tagttgcaat ggggcgatga gccacaattg ctttagctat tctgcgtgct
     1441 tttggctctt cgccataatt acgcaatatc tgctctaggc tttccgcact ccactcgttt
     1501 acgactgttt cagccgtcaa tgtttgacgt tgatccattc gcatatctag tggtcctata
     1561 gcagcaaagc taaacccacg actcgccttg tcaaggtgca gtgacgatac gcctaaatct
     1621 gccaatatta gatcaaattg ctggcctgct gctactaaat cttccgcagc agagtaaaaa
     1681 tcgttccgca ctatttttac tctagtagta gcaaattcgg ccattaagtg gtctaccgca
     1741 gctttatcgc gatcaaccag aacactttca gaaaagttat gggtatgcgc taagatttcc
     1801 cgcgcatgcc caccataccc agcggtggca tctaagtacc tttccccgct cgtggggttc
     1861 attgcagcta taacttcttg tagtaatact ggttcatgca tattttttgt ttttattttt
     1921 gcaaggtcac cgattcagta gccaacgttt tgtttttttg tagtttttgt ttttgtataa
     1981 agtgtatagt ttttggtgat atatcatttt gactgactat ccaaatgtga atttgaaatg
     2041 tatgtcggct tttatagatg tatcgctata gactttgtag agacttatgt gtgaggtgga
     2101 gttttgggag gtgttttgct taagtaaggt gcgaggattt ttaacgtagt tcctaataat
     2161 gctacgtgtt gactaccgaa tcgctaatct cgagtcggtg tggtttggtg tgttaaattg
     2221 ttaatgttcg tagattattt agcagagagg cgccaatagg cccctgcccg tactgcaaca
     2281 acgtctttgg taatgccggc aaagtctaac agatgctgtt caagggtaac cctaccctgt
     2341 ttttgatcta gctctgcgct ggttttgcca ctgcgaaatt gtacatttaa gtcggccact
     2401 ttctcgtcga gaatatagcc tgtaagttcg ggttctactt gtgaattcca aacttcttct
     2461 gagtacatat gcagatacgt accaaatcct tttgttacga caacaccgct tttgaactcg
     2521 ctccgaagct cggccggtat tgttaaccga cgcttatcgt caagcttcct ttcgaagtag
     2581 tctacggtgg tcatttttta atctacttag tggttttttt attttgtgct gtggtgttac
     2641 ccacttttac ccactaagta cagtctacta cccactacta cccactgcaa gtacttttta
     2701 tgtatttatg tataaagtg
//
LOCUS       DAOPLS010000095         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_43448_length_2685_cov_3.797719, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000095 DAOPLS010000000
VERSION     DAOPLS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43448_length_2685_cov_3.797719"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKV52_02655"
     CDS             complement(<1..941)
                     /locus_tag="PKV52_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPD99145.1"
                     /translation="MSGVLWGFSPLIYKNSLLFFGFVSLLAIRFLVGAGIVYALSFRK
                     FIKISKRHLVLIIIMSLTSSVASMFLYLSGLKLTSAMHASIIGLSLPFFVYLLSVLIL
                     KEKVHRVVLIGSVLASAGLLVIIFGSNTNNGQASIAGDVLILLSEVFFSIGVILARKI
                     VTGTKKLPPEQLVFADYLLAGIVFAVAAFIVGNGSSMPTFTPIAILWLLLVVTVGGFL
                     PNILFLKSARRLPAEKLADTNFITPIVGIMLAVIFLNESMNVSFLIGGTLVFLGLTIS
                     NKKLTPRLPVLTEEVKAIELAFIHKSQALERIIYNQY"
     gene            complement(1031..>2685)
                     /gene="uvrA"
                     /locus_tag="PKV52_02660"
     CDS             complement(1031..>2685)
                     /gene="uvrA"
                     /locus_tag="PKV52_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002185582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPD99146.1"
                     /translation="ESEFIKKDIEKFMLQQPCHACKGARLKPAVLAVTVKNMSIIDVV
                     NLSIDDAIEFFDTIILTDTEKTIAHQILKEIRARLTFLRDVGLNYLSLSRSAVTLSGG
                     EAQRIRLATQIGSGLTGVLYVLDEPSIGLHQRDNERLIGTLKKLRDLGNTVLVVEHDE
                     ETIRTADYLLDIGPGAGIHGGEVVAHGTPKEVEKNSSSITGQYLSGIRTVPMPKKQRA
                     GNGKELVIVGAKENNLKNLTVTIPLGKLVVVSGVSGSGKSSLVNDILAKELQARLHRA
                     NLVAGKHTEIAGLKNLDKAIVIDQSPIGRTPRSNPATYTGLFTPIRELFSNTPESKIR
                     GYSPGRFSFNVKGGRCENCAGDGIIKIEMHFLPDVYVPCEECHGKRYNREALEIHYKD
                     KTISDVLAMTCEQALDFFENIPAVKRKLQTLVEVGLGYIQLGQSATTLSGGEAQRIKL
                     ATELSRRPTGKTLYILDEPTTGLHIDDVNRLLKVLHALVDTGNSMIVIEHNLDVIKSA
                     DYIIDMGPEGGEAGGKVVAEGTPQQIAKVKTSYTGQYLKKIL"
BASE COUNT          796 a          577 c          478 g          834 t
ORIGIN      
        1 ttgtattgat tatatataat tctttctaaa gcctgagatt tgtgtataaa ggccaattct
       61 atggctttaa cttcttctgt tagcacaggc aaacgaggtg taagtttttt gttactaata
      121 gttagcccaa gaaaaactag tgttccacca ataagaaatg aaacattcat actttcgttc
      181 aaaaatataa cagcaagcat aatacctact attggagtaa tgaaatttgt atctgctagc
      241 ttttctgcag gtagacgacg agcgctttta agaaacagga tattcggcag aaaaccacct
      301 accgttacaa ccaacagtag ccacagtatt gctattgggg taaatgtcgg catgctactg
      361 ccattcccca ctataaatgc tgcaaccgca aatacgatgc cggctaataa ataatctgca
      421 aagacaagct gttctggagg taacttttta gtacctgtga caattttacg agctagtatt
      481 accccgatag aaaaaaatac ttccgaaagc agaatcaaca catcgccagc aatactggct
      541 tgcccattat tcgtatttga accaaatata atgacaagta accctgcgct tgcgagtaca
      601 ctacctatca atacaactct atgaactttt tcttttaata tcagtactga taataaatat
      661 acaaaaaatg gtagagataa accaataata gatgcatgca tcgcacttgt taattttagc
      721 ccagataagt ataaaaacat tgacgccaca gaagacgtaa gtgacataat tattatgaga
      781 accagatgtc ttttgcttat tttaatgaat ttacggaaag acaaagcgta cacaatgcct
      841 gcgcctacca aaaatctaat cgcaagcaat gaaacaaacc caaaaaataa taaactattt
      901 ttatatatta aaggtgaaaa accccataag acaccgctca taacgagcaa tttataatat
      961 ttattgtgaa atattttaat ttttatcact atagtgttag tatagtcgat taattgctac
     1021 ttaagcaata ttataagatt tttttaaggt attggcctgt atagctagtt ttaacttttg
     1081 caatctgttg cggagtgccc tcagccacta ccttgccgcc ggcttcgccg ccctctggac
     1141 ccatatctat aatatagtcg gcacttttta taacatctaa gttgtgttct ataacaatca
     1201 tagagttgcc cgtatctact aacgcatgca aaacttttag taacctattt acatcgtcta
     1261 tatgtagccc agttgtcggt tcgtctaata tatacaaagt cttgccggta ggcctgcgag
     1321 atagctctgt ggcaagctta attcgttggg cttcgccacc acttaatgtc gttgccgatt
     1381 ggccaagctg tatataacct aagccaactt ctacgagtgt ctgtaacttg cgctttaccg
     1441 ctgggatgtt ttcaaaaaag tctaacgctt gctcacatgt catcgcaagc acatcactta
     1501 ttgttttgtc tttataatga atttcgagcg cttcgcggtt gtagcgttta ccatgacatt
     1561 cttcgcacgg gacataaacg tctggtaaaa aatgcatttc tattttaata attccgtcac
     1621 cggcacagtt ttcacagcgc ccacctttta cattaaagct aaatcggcca ggactgtatc
     1681 cacgtatctt tgattcagga gtgttagaaa atagctctct aataggtgtg aataacccag
     1741 tgtaggttgc tgggttactg cgcggcgtcc gaccaattgg tgactgatca attactattg
     1801 ctttatctaa gttctttaat cctgcaattt cagtatgttt cccagcaacc agatttgccc
     1861 gatggagcct cgcttggagc tctttcgcta agatatcatt gacgagacta gactttccag
     1921 atccactcac accacttact accactaatt tacccagtgg tatcgttacg gttaagtttt
     1981 ttaaattatt ttctttggca ccaacaatta ccagctcttt gccgttacca gcacgttgtt
     2041 ttttgggcat aggtacagtt ctaataccac tgagatactg gccagtgata ctgctgctgt
     2101 tcttttctac ttctttcgga gtaccatgag cgacaacttc tcctccgtgt ataccagcac
     2161 ccggccctat gtctaataaa taatcagctg tacgaatcgt ttcttcgtca tgttcaacaa
     2221 ccaatacagt gttgccgagg tcacgtaatt ttttaagcgt acctatgagt ctttcattat
     2281 cgcgttgatg caaaccaatt gacggctcat ctagcacata aagcacgcct gttaagcccg
     2341 acccaatttg cgtggctagc cgaatacgct gagcctcacc accactaagg gtgactgcgc
     2401 tccgtgatag cgataaataa tttaagccta cgtcgcgcaa gaatgtaaga cgagcgcgaa
     2461 tttctttaag tatttgatgc gcgatcgttt tttcggtatc tgtaagaatg atagtatcaa
     2521 aaaattcaat cgcatcatcg atagatagat ttactacatc aatgatggac atgtttttaa
     2581 cagtaactgc taatactgct ggctttaatc gggcaccttt acaagcatgg cacggttgtt
     2641 ggagcataaa tttttcaata tcttttttaa taaattcgct ctctg
//
LOCUS       DAOPLS010000096         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_44093_length_2658_cov_2.845179, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000096 DAOPLS010000000
VERSION     DAOPLS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44093_length_2658_cov_2.845179"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKV52_02665"
     CDS             complement(<1..605)
                     /locus_tag="PKV52_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPD99147.1"
                     /translation="MANSTLDLEHAPAHYEPQYGSASLPYPLPLNTIHSYEAIELGNP
                     DLPIYPHKSEIKSTVLLNDLQTIIRSGTGSGKSTQVVQYMLEAGFSKVIITQPRIVAA
                     RTLKDRISQELSWKIDGLPDDFVGYRTANEGDSTHESVVEIVTDGLEVRRLLSSGGLG
                     QDTILIVDEVHEANANITMLLALIKRIGIRAVIMSATFNTEG"
     gene            833..1489
                     /locus_tag="PKV52_02670"
     CDS             833..1489
                     /locus_tag="PKV52_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPD99148.1"
                     /translation="MKNNFVYVFMAIIVGGVFLYVLNISSSNSLEADQMNNRDLTKFT
                     QGNLDAPVKVTQYSDFLCPSCSQVSLGIVPKIIEKYVTTGNVYYTFVPMAFIAPGSQL
                     AAEGAFCAAKQDKFWEYHDVAYQAVWEDYFSKGVDPSRVPLYSEAGVKQLGQLAQVDT
                     DSFNSCIDNRVEKATVMAITQDAQNNGVQGTPYFVVNDTPIKGVPSFEILDAAISTKL
                     "
     gene            1494..2090
                     /locus_tag="PKV52_02675"
     CDS             1494..2090
                     /locus_tag="PKV52_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99149.1"
                     /translation="MIQRLQLLKTKAQLSLKASSNVLKSPKYLLFAAIVALLFIQLLY
                     WLLNASLFWYFFTARSLNIIEKMDIFSSIIVSYLSSLPLWQAFAVVALAITQGVVISV
                     LLFTVRNNQSVDKKAVGGTGIASIIAMLSVGCVSCGTSIVAPIIGLFVSGATASLSEA
                     INKIAIVVGLIIALYALYAVGLTAANIQAKQKSTSTTS"
     gene            complement(2080..2643)
                     /locus_tag="PKV52_02680"
     CDS             complement(2080..2643)
                     /locus_tag="PKV52_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPD99150.1"
                     /translation="MNDNIDPQLAQNGDPRNVQDTYKNWKLEAIKNDLQAKAFGVHVA
                     IENYQHDYNIGTIVRSANAFNVAGVHIIGKRHWNRRGAMATEKYLQLYHHPTVVDFIN
                     WVAPTTLVIIGIDNIANSIPLHQANIPENAILVFGQEGPGLSVEMQSYCKRIIAIEQF
                     GSTRSINVGVAAGIVLYEWVRRHHITM"
BASE COUNT          753 a          534 c          539 g          832 t
ORIGIN      
        1 ccttcagtat taaatgtagc gctcataatg actgctcgta tacctatacg ttttataagt
       61 gccaagagca ttgtaatgtt agcgtttgcc tcgtgtactt cgtctacaat aaggatcgtg
      121 tcctgtccta gcccaccact acttaataac cgccgtactt cgaggccgtc tgtaacaatt
      181 tctacaacgc tctcatgtgt actatcacct tcgttggctg tgcggtagcc aacgaaatca
      241 tctggcagcc catctatctt ccaagataat tcctggctaa tacgatcttt aagagttcgc
      301 gccgccacga tacgaggttg ggttatgata actttagaaa atccagcttc aagcatatat
      361 tgcacaactt gagtagactt gccgcttcct gttccagatc gaattattgt ttgcagatca
      421 tttaataaaa cggtagattt tatttcagac ttatgtgggt aaattggcaa atctggatta
      481 cctagttcaa tagcttcgta ggaatgtatc gtatttagtg gtagtgggta ggggagtgat
      541 gcagagccat attgtggctc ataatgtgcc ggtgcgtgtt ctaggtctag cgtgctattc
      601 gccacgcctt gtcgctttct gttttgaata tttttatata catattgtta tgtcaaaact
      661 ttcttttgtt taccgacagc tctcacactg ttactttcaa tatacaccca aaaatgtgta
      721 tgtatacaaa gaatgtaaat accacaacaa taaggttatg cttaccacta gaaattatta
      781 ttgtaaataa tttacaataa ggttatactt accatatcaa ggagtagtct tagtgaaaaa
      841 taattttgta tacgtattta tggcaattat tgtaggcggg gtatttctat atgttctgaa
      901 tatctctagt agcaacagcc tagaagctga tcagatgaat aatcgtgatt tgacaaaatt
      961 cacccaaggt aatctagatg cacccgttaa ggtgacccaa tattcagatt ttctgtgccc
     1021 atcttgttcg caagtaagtc tggggatagt accaaaaata atagaaaagt acgttacgac
     1081 aggcaacgta tactacacgt ttgtcccaat ggcatttatt gctccgggtt ctcagttggc
     1141 agctgaaggt gctttttgcg cagccaaaca agacaaattt tgggagtatc acgatgtggc
     1201 atatcaagct gtctgggaag actactttag taaaggtgta gatccatctc gggtaccact
     1261 ttatagtgaa gcaggggtaa agcaactcgg ccaactcgct caagtagaca cagatagttt
     1321 taatagttgt atagataaca gggttgaaaa ggctactgtt atggcgataa cgcaagacgc
     1381 gcagaacaat ggtgttcaag gtacaccgta ttttgttgtt aatgacactc ccatcaaggg
     1441 tgtacccagc tttgaaatat tagacgctgc aattagcact aagttataac agcatgatac
     1501 aacgtctaca actactcaaa actaaggctc aattgtcatt aaaagccagc agcaatgtgc
     1561 tgaagagccc aaagtattta ctcttcgcag ctatagtcgc gctgctattt attcagttac
     1621 tgtattggct tctcaatgct tcgctatttt ggtatttttt cacggctcgc agtctaaaca
     1681 tcatagaaaa gatggatata tttagcagta ttattgtcag ttatctttct agtctaccct
     1741 tgtggcaggc atttgctgtt gtagctctcg ctattacgca gggagtcgtt attagtgtgc
     1801 tgctatttac ggttcgcaac aaccaaagcg tggacaaaaa ggcagttggt ggtacgggca
     1861 tcgcaagtat aatcgcaatg ttgagcgtag gatgtgtttc gtgtggtact tctatagtgg
     1921 cgccaataat tggcttattt gtttctgggg caactgcaag tttaagcgaa gccattaata
     1981 agatagccat tgtcgttgga ttaattattg cgctttatgc attatatgca gttgggttga
     2041 ccgccgccaa tatacaggca aaacaaaaaa gtaccagcac tacatcgtaa tatggtggcg
     2101 ccgtacccac tcatacaaca ctatacccgc cgccacgcca acgttaattg agcgcgttga
     2161 accaaactgt tctatcgcaa taatacgttt gcaataagat tgcatttcta ccgataaacc
     2221 aggaccttct tgaccaaaca ctaatatggc attttctgga atatttgctt gatgtagcgg
     2281 tatgctatta gcaatattat caatgccaat aatgactaat gtcgttggcg ctacccagtt
     2341 aataaaatct acaactgttg ggtggtggta aagctgtaag tatttttctg tggccatagc
     2401 gccccgccga ttccaatgtc gtttgccaat aatatgtaca ccagctacat taaaagcatt
     2461 agcactgcga acaattgtac caatgttata atcgtgttgg taattttcta ttgccacatg
     2521 cacaccaaaa gccttggctt gtaagtcatt tttaatggct tctagtttcc agtttttata
     2581 tgtatcttgt acatttcgcg gatcaccatt ttgcgctaac tgcggatcta tattatcatt
     2641 catcattcat ctagagca
//
LOCUS       DAOPLS010000097         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_45378_length_2608_cov_2.213083, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000097 DAOPLS010000000
VERSION     DAOPLS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45378_length_2608_cov_2.213083"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..491
                     /locus_tag="PKV52_02685"
     CDS             207..491
                     /locus_tag="PKV52_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99151.1"
                     /translation="MANVLLSIKTDAATKKAVKEFAGELGVSTTALINMVLKQTLRDK
                     KIVLSTASEPTPYLEKIITMADVDYKEDKDITHTKGAKEALAHIDSLMNK"
     gene            495..755
                     /locus_tag="PKV52_02690"
     CDS             495..755
                     /locus_tag="PKV52_02690"
                     /inference="COORDINATES: protein motif:HMM:NF027074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HPD99152.1"
                     /translation="MNIDFTKSFNKQFAKLALKKQERARAAIELYVCDASDPKLRLHA
                     LVGTWQGHYSISAGGDLRLHLKIIDNNTILFVAIGSHSQLYK"
     gene            1226..1501
                     /locus_tag="PKV52_02695"
     CDS             1226..1501
                     /locus_tag="PKV52_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99153.1"
                     /translation="MKSIQYTIRNVPPKVDRYYKKIAKISGKSLNQVVLDKLSEKSGQ
                     GKNKTLLDSLDWFIGSNSIDEATYRAWEEDDKIQKELTRKQWLKDDN"
     gene            1491..1883
                     /locus_tag="PKV52_02700"
     CDS             1491..1883
                     /locus_tag="PKV52_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPD99154.1"
                     /translation="MIIDTNVYSALDRADPVVLQMLAGAPMAYIPIIVLGELRYGFTK
                     GSRTSDNEIRLKRFISLDTVETIYLTDQTAKVYGELSVLCSKKGRALSNNDVWIAALA
                     LENDMPLVTFDKDFEVFADVLGDGLIIL"
     gene            1880..2011
                     /locus_tag="PKV52_02705"
     CDS             1880..2011
                     /locus_tag="PKV52_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99155.1"
                     /translation="MRKLNYGYSINDRAEWADANRRDKEVPDSELEDWDITVGDGVE"
     gene            complement(2059..2571)
                     /locus_tag="PKV52_02710"
     CDS             complement(2059..2571)
                     /locus_tag="PKV52_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD99156.1"
                     /translation="MIIDNPEEVLDLVNEHDVVVGTILRAEVPQLAATSKGFVRAVGV
                     FLQNSKKQLFVPARSLHKKLLPGAYDFSAAGHVASGQSYIEAAVQETAEELNIQLNTS
                     SFRLIGTLAPLNGIPYFNHIFIVEYEETPLYNKEDFYKAEWLYPEELIESIKSGHPAK
                     QVILPALKLL"
BASE COUNT          868 a          450 c          499 g          791 t
ORIGIN      
        1 ccctttccca tgagctatac gataccccgg accccatatt tgtcttggcc atgattatga
       61 ctacaacaaa taaataaatt actacaaaga tgcactgata atgagaagct ccgtcaaaaa
      121 atattacata ccgcggtttt ccaaacagat ataggttgcg ttttattatt gacaaataca
      181 tacaatgtat atataatgta tgcgttatgg ctaacgtatt attgagtatc aaaacagacg
      241 cagctaccaa aaaggcagtc aaagaatttg ctggtgagtt aggcgtatct actacagctt
      301 taattaatat ggttttaaag caaactttac gtgacaaaaa aatcgttcta agcacagcca
      361 gtgagccaac accttaccta gaaaaaataa ttactatggc agacgtagac tataaggaag
      421 ataaggatat tacccacaca aagggagcta aagaagcttt ggcgcacata gatagtctaa
      481 tgaataagta gtatatgaat atagatttta caaaatcttt taataaacaa tttgccaagt
      541 tagcattaaa aaaacaagaa agagccagag ctgcaataga attatacgta tgcgatgcat
      601 ctgacccaaa gctgcgtctt catgccttgg ttggtacatg gcaaggacac tattcaatta
      661 gtgccggagg cgaccttagg ctacatctta aaataattga taataacaca atactttttg
      721 tagctattgg tagtcatagc cagctatata aatagtttat atatatattt tttctttaca
      781 cttttccaaa aaaattaact cttactttgc aatgcccaaa gtaacaaagc ctctgcgctt
      841 tgtagttaat tcttaagata ataaattatt tcaaaactat ataaatacaa ctttgggcag
      901 caaagttggg taaactgccg gggtgcgtat agctctttgc gtaagggctt gtaaaagaca
      961 acaactacaa aaagaactca ccattctatt attatttaag tacagaatgg cttaacacct
     1021 ttttgtttaa tagttgttgc ttttacgccc tttcccatga gctatacgat accccggacc
     1081 ccatatttgt cttggccatg atgatgacta caacaaataa atcaattact acaaagatgc
     1141 acctagcagc gtcttattaa ttcaaatatt acgtacttaa tttgtaaata ttgtaaaaag
     1201 caggcatgca tgctataatg taggcatgaa atcaatacag tatacaatta gaaatgtacc
     1261 accaaaggta gataggtact ataaaaagat agcgaaaatt agtggcaaaa gcctaaacca
     1321 ggttgtgctt gataaattat ccgaaaaatc tggccaaggc aagaacaaaa ctttactaga
     1381 ctcacttgat tggtttattg gtagcaatag cattgatgaa gcgacatacc gtgcatggga
     1441 agaagatgac aaaatacaaa aagagctaac acgcaaacag tggctaaaag atgataattg
     1501 ataccaatgt ctatagcgcg ctagatagag ctgacccagt tgtgctgcaa atgcttgctg
     1561 gcgcacccat ggcgtatata ccaataattg ttttgggtga gctaaggtat ggttttacta
     1621 aaggctcaag gacttcagat aacgaaatta gactaaagcg ttttatatct cttgatactg
     1681 tagagactat ttatttaaca gaccaaacag caaaagtata tggagagctt tctgttctat
     1741 gttcaaaaaa agggcgagca ttaagcaata atgatgtttg gattgcagct ttagctttag
     1801 aaaacgatat gccactggtt acttttgata aagattttga agtatttgcc gatgttttag
     1861 gtgatgggct aataatattg tgagaaaact taattatggt tattccatta atgatagagc
     1921 cgagtgggca gatgcaaacc gtagagataa agaagtccca gatagtgagc tagaagattg
     1981 ggatatcact gtcggtgacg gtgtagagta gcgcatatca caataaatgg actatatgtt
     2041 tgccgagtaa ggatagacct ataagagctt aagtgcgggt aatattactt gttttgctgg
     2101 gtggcctgat ttaatacttt ctattaattc ttctgggtat agccactctg ctttataaaa
     2161 atcttcttta ttgtataagg gggtttcttc gtattccacg ataaatatat ggttaaagta
     2221 gggtatgcca tttaatggcg ctagagtacc aattaaccta aatgagctgg tgtttaattg
     2281 tatattcagt tcttctgctg tttcttggac tgcagcttct atataagact ggccactagc
     2341 tacgtgacca gctgcactaa agtcgtatgc gccaggtagt agctttttat gcaatgacct
     2401 tgctggaaca aatagttgtt ttttagagtt ttgtaaaaat acacctacag ccctaacaaa
     2461 gcccttgctg gtagctgcta attgcggtac ctccgctctt agtatggtac ctacgactac
     2521 atcgtgttcg ttaacaaggt ctaatacttc ttctgggtta tctataatca tttattcgtc
     2581 attgagttct tctatgtagt tatttaga
//
LOCUS       DAOPLS010000098         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_45443_length_2605_cov_4.039608, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000098 DAOPLS010000000
VERSION     DAOPLS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45443_length_2605_cov_4.039608"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1434)
                     /gene="dnaB"
                     /locus_tag="PKV52_02715"
     CDS             complement(67..1434)
                     /gene="dnaB"
                     /locus_tag="PKV52_02715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPD99157.1"
                     /translation="MAKQKEAIIPPHNTEAESSLLGAILIDTDAIVRVADIISVEDFY
                     DERHQRIFEAIKKLYEKHSPIDVLTLSDQLKSSGFLDIIGGAGYLTELTNYVPTAAHV
                     EEYANIVAQKAIRRRLIKASQTMVTFGYDEEKPIQDLIESAESELFQVSQQHVKQDIS
                     SIEDILAESFERLDELHKDKGKIRGIPTGLRDLDNILAGLQRSDLIILAARPSMGKTA
                     LTLNLAHNIATKTELPVLVFSLEMSKEQIVDRMLSMESGVDAWNIRTGNLSDTDFEKI
                     GQAMGTLSEAPIYIDDTPGITVSDMRTKARREQHQRQLGAIMVDYLQLMSGGSRFGSD
                     ANRVQEISEISRGLKAIARELNVPVVALSQLSRSVESRSPQIPQLADLRESGSIEQDA
                     DVVAFIYREDYYNPDTDRKNLTDIFIKKHRNGPTGQVELYFDRERQRFKSLDKRQASS
                     HVPFE"
     gene            complement(1446..>2605)
                     /locus_tag="PKV52_02720"
     CDS             complement(1446..>2605)
                     /locus_tag="PKV52_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99158.1"
                     /translation="LKTLEEPPAHVIFILATTESHKLPETITSRTQRYNFTLATQKEV
                     VDHLADICTKESIDIEPSALTVIAQSSGGSLRDALSILDHARHIGDVITADDIRQHIG
                     VPSDEIVHDILSALRAQDAALVLEHLHEAQVSAVSSAALSRSLIDELTKQIQNNTTAM
                     PLEYITQLLQGLLQVELSNQEDVRLRVALLQSIHPTDNSHKVTDLSLSPKKTAHQSAA
                     PVRKVHADDDTKRASKKSQSSAVKELPPSQVATPKTNIAVPKEHQSIDTKELTDEGWH
                     SLLEDVRTTHNTLYSVLRMAEVDYSKANNNTIRLSFAFPFHKKRINESKNKDIISQKL
                     ASLGYGNFEIVCDNDSDQRIASTVINRSVSEEKDAMLSKIRGVFGSAEVLE"
BASE COUNT          652 a          523 c          525 g          905 t
ORIGIN      
        1 acaaaaccgc gccaatgctc tttaaaccat gattttgatt gttttattaa gtgattgatc
       61 ttcatattat tcaaatggta catggctaga ggcttgacgc ttgtctaggc tcttaaatcg
      121 ctgtcgttct ctatcaaagt ataattcaac ttgcccagtt ggcccgttgc ggtgtttttt
      181 aataaatata tctgttaggt tttttctgtc tgtatctgga ttgtaatagt cttctcggta
      241 aatgaacgct accacatctg catcctgttc aatagaccca gattcgcgta agtcggccaa
      301 ttgtggaatt tgtgggctgc gcgattcaac agaacgactt aattggctta atgcgacgac
      361 tggtacgttt aactcacgtg cgattgcctt taagccacgg ctgatttcag atatttcttg
      421 aacccggttt gcatcactgc caaatcgtga gccaccactc atcagttgta ggtagtcaac
      481 cataatggca cctaactgcc gttggtgttg ttcacgacgc gccttggtac gcatatcgct
      541 gaccgttata cctggtgtat cgtctatgta tataggagct tcagaaagcg ttcccatagc
      601 ttggccgatc ttttcaaaat ctgtatcaga tagattacct gttctaatat tccatgcatc
      661 tacaccagac tccatagata acatacgatc aacaatttgt tctttgctca tttctaggct
      721 aaatacaagc acaggcagct cagtttttgt cgcgatatta tgtgccaaat taagcgttaa
      781 agcggtttta cccatacttg ggcgggcagc taagattatt aagtctgagc gctgtaatcc
      841 tgctaatata ttgtctaaat cacgcaaacc ggttggtatc ccacgtatct tgcctttatc
      901 tttatgaagt tcgtctaaac gttcgaaact ttctgctaag atatcttcta tagatgatat
      961 gtcttgtttg acgtgttgtt ggctcacttg aaatagttcg gattcggctg attcaataag
     1021 gtcttggata ggcttttctt cgtcatagcc aaacgtcacc atagtttgtg atgctttaat
     1081 aagccgacga cgaatggctt tttgggcaac gatattcgca tattcttcca catgtgcggc
     1141 agttggcacg tagtttgtca gctctgttaa ataacctgct ccgcctatta tgtctaaaaa
     1201 gccactagac tttagttgat cagacaatgt taagacatca attgggctgt gcttttcata
     1261 taatttttta atagcttcaa atatacgctg atgtctttca tcataaaaat cttctacaga
     1321 aataatatca gcaacacgta caattgcatc tgtgtctatc agaatagcac ccagcaagga
     1381 actctctgcc tctgtgttat gcggaggtat aattgcctct ttttgctttg ccatcactct
     1441 taattttact ctaatacttc agccgagcca aaaactccgc gtattttact taacatagca
     1501 tctttttctt cgctgactga tctgttaata acagtagagg ctattctttg atctgagtca
     1561 ttatcacata caatttcaaa attgccatac cctaggctgg cgagcttttg gcttataatg
     1621 tctttgtttt ttgattcatt gattcttttt ttatggaatg gaaaagcaaa acttagacgt
     1681 attgtattgt tattagcttt actataatct acttcagcca tccgtaaaac actatagagt
     1741 gtattatgtg tcgtcctaac gtcttctaat aatgaatgcc atccttcatc tgtcaactct
     1801 tttgtatcta ttgattgatg ttcttttggc acggctatgt tcgtttttgg tgtagctact
     1861 tgtgaagggg gtaattcttt tacggcacta ctctgtgatt ttttagaagc gcgcttggta
     1921 tcgtcatctg cgtgtacttt acgtacaggt gctgcacttt ggtgagctgt tttcttaggg
     1981 cttaaggaca aatcggttac tttatgtgag ttatctgtcg gatgaatact ttgtagtagc
     2041 gcaacacgca aacgaacatc ctcttgattg gaaagttcta cttgcagtaa tccttggagt
     2101 aattgggtaa tatactctag tggcattgct gtagtgttat tttgtatttg ctttgtaagt
     2161 tcgtcaatga gtgaacgaga cagtgcagct gatgacactg cacttacttg agcctcgtgt
     2221 aggtgttcga gtactaaggc ggcatcttgt gcacgcaaag cactaagtat atcatgtacg
     2281 atttcatcac ttggaacacc aatatgttgc ctgatgtcgt ctgcagtaat aacgtcacct
     2341 atatgacgag cgtggtctaa tatacttagt gcgtcacgca aactgccacc acttgattgt
     2401 gcgataactg ttagcgcgct aggctctatg tctatggatt cttttgtaca aatatcagca
     2461 aggtggtcta ctacttcttt ttgggtggct aaggtaaaat tgtatcgttg cgtccttgat
     2521 gtgattgttt ctggtagttt atgactttct gtagttgcaa gaataaaaat tacgtgtgcg
     2581 ggcggttctt ctagtgtttt caaca
//
LOCUS       DAOPLS010000099         2589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_45873_length_2589_cov_3.520916, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000099 DAOPLS010000000
VERSION     DAOPLS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45873_length_2589_cov_3.520916"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PKV52_02725"
     CDS             complement(<1..712)
                     /locus_tag="PKV52_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019777014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD99159.1"
                     /translation="MVKSAVVKVKKLVKKYDNKKVVDGVSFSIAQGEIFGILGPNGAG
                     KTTTLEMIEGLRLIDAGEAYIDGINVATHPTDIRYRIGVQPQSPAFQDKTKAVEVLDM
                     FAAAYGVKSSSHDLLQKVSLLDKASTYTEQLSGGQLQRLSIAAALVHSPKVLFMDEPT
                     TGLDPQARRNIWELVKSIKANGVTVVLTTHYMEEAELLCDRVAIMDKGVVIAVDTPQI
                     LVEQLLMRGFKKKRQVNQA"
     gene            complement(799..999)
                     /locus_tag="PKV52_02730"
     CDS             complement(799..999)
                     /locus_tag="PKV52_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99160.1"
                     /translation="MAYQHTNSKGVTYHLNSKDVVLRGGKKQTIYYFSKDERPEACDL
                     PDGMSVNENPRNGFLTVKRDKK"
     gene            1166..1753
                     /locus_tag="PKV52_02735"
     CDS             1166..1753
                     /locus_tag="PKV52_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99161.1"
                     /translation="MEQLNRMNRPAPAGGQVPVQAPVNHGGASKNKKPKVGTIAKIAA
                     VAAVVVLLIALVFVFVIKSTQGISAEINKDKYQAVFLNSADGQVYFGKLSELNGDYYK
                     LNDIYYVRVEQVQPNPNEQPTQNISLAKLGNEIHGPEDVMYIRKEHVMFWENLKDDGQ
                     VVTAITEYKKNGGNPSQTQNQNQNQPQQPQQDTNN"
     gene            complement(1806..2414)
                     /locus_tag="PKV52_02740"
     CDS             complement(1806..2414)
                     /locus_tag="PKV52_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99162.1"
                     /translation="MINKSKAHHYWKAIRRPSLFVIVAGFVLFSALSIYGLRINNTRM
                     LELKQAVFTADEQNGDIEAALQNLRNHVNSHMNTQLRAPDATEPPIQLVNQFNRYVQA
                     EQLKLANQDTNKVYQDAQARCETGAIPLTARAQCIQEYIIANGGNAPQLSLPPKELYT
                     FDFASPKWSPDLAGISIVLAITFFTLLVIRLIVGRYMKRELQ"
BASE COUNT          783 a          531 c          503 g          772 t
ORIGIN      
        1 tggcttggtt tacctgacgt ttctttttga accctcgcat taacaactgt tctacaagta
       61 tctgaggagt atctactgct atcacgaccc ctttatccat aattgctaca cggtcacaaa
      121 gcaattcagc ttcttccata taatgcgtag taagcactac agtaacaccg ttggctttga
      181 tgctttttac gagctcccat atgttacgac gtgcttgtgg gtctaagcct gtagtgggtt
      241 catccataaa tagtactttc ggactatgta caagtgctgc agcaatgcta agccgctgca
      301 attgcccacc agatagttgc tcggtatacg tagaggcttt atctaggaga cttacttttt
      361 gaagcaaatc gtggctacta ctttttacac cataagcagc ggcaaacatg tctaatactt
      421 ctaccgcttt agttttatct tgaaaggctg gtgattgagg ttgcacacca atacggtaac
      481 ggatatctgt tgggtgtgta gccacgttga tgccgtctat atacgcctcg cctgcatcaa
      541 taaggcgcaa gccttctatc atttctaacg tggtcgtttt accagcaccg ttagggccta
      601 atataccaaa gatctcgccc tgagcgattg aaaaagaaac accgtcaaca actttcttgt
      661 tatcgtattt ctttacaagc ttttttacct tgacgaccgc tgattttacc atatatctat
      721 aactatacgc tgcttcatat aaaaagtcag cgacccacta aacgtgatcg ctgactaatg
      781 tattctctta gtaatttgct attttttgtc gcgctttact gtcaagaaac cgttacgagg
      841 attttcgttt acgctcatac cgtctggtag atcgcatgct tcaggtcgct catccttact
      901 gaagtaataa atagtttgct tcttgccacc gcgtagaact acatcttttg agttcaagtg
      961 atatgttaca ccttttgagt tcgtgtgttg atacgccata ctcttctcct tacactgtta
     1021 gtagtacttt taccataccc tcttgtggta actgttgtca acaggtttta tgtgagttga
     1081 aaaaaagttg ttgccaaaag ccttatgctt gattacacta taaggtacat aaactgttaa
     1141 ttaatataaa gaggtaaagg ggtatatgga acagttaaat cgtatgaata ggccggctcc
     1201 agctggtggg caagtacccg tgcaagcacc tgtaaaccac ggtggtgcat caaaaaataa
     1261 gaaacctaag gtaggaacaa ttgcaaagat tgcagcagtt gcagctgtag tagtacttct
     1321 cattgcacta gtctttgttt ttgtaataaa aagtacccaa ggtatttcag cagaaatcaa
     1381 taaagacaag taccaagctg tgttcttaaa tagcgcagat ggtcaagtat actttggtaa
     1441 attaagtgaa ttaaatggtg actactacaa gctcaacgac atttactatg tacgtgtaga
     1501 gcaagttcag ccaaatccaa acgaacagcc aactcaaaat atatcacttg cgaagttggg
     1561 taacgaaata cacggtccag aagatgtaat gtacatccga aaggaacatg taatgttctg
     1621 ggaaaaccta aaagatgatg gtcaagttgt tacagcaata actgagtaca agaaaaacgg
     1681 cggtaatcct agccaaactc agaaccagaa ccaaaatcaa ccacaacaac cacaacaaga
     1741 taccaacaat tagttagtac tttactgatt acaataaaaa agctccccgt aaacgaggag
     1801 cttttttatt gtaactctcg cttcatatac ctaccgacta ttagccgaat gactaatagg
     1861 gtgaaaaagg tgatggctag tacaatagat atgccagcta aatcgggtga ccacttaggg
     1921 ctagcaaaat caaacgtata gagttctttt ggtggtaaag acaattgtgg tgcgttacca
     1981 ccgtttgcaa ttatgtattc ttgaatacac tgtgcccgcg ccgtaagagg tatcgcaccc
     2041 gtttcacaac gtgcttgtgc gtcttggtat accttattcg tgtcttgatt agcgagctta
     2101 agctgttctg cttgtacgta gcgattgaat tgatttacca attgaatagg tggttctgtt
     2161 gcatctgggg cacggagttg cgtgttcata tgtgagttca cgtggtttct aagattttgc
     2221 aaagctgctt ctatgtcacc gttttgttca tctgctgtaa aaactgcttg cttaagttcg
     2281 agcatacggg tgttgtttat acgaagccca tatatactta gagcactaaa taatacaaaa
     2341 ccagcaacaa taacaaataa tgacggcctg cgaatagcct tccagtaatg atgcgcttta
     2401 gatttattaa tcatacttgt atattacctt aatgctgcaa aacgttgcgt taaaatacct
     2461 aatgaactaa tagcgccata tacaaataat gtttgcaaaa gcactttttc atcatgaata
     2521 aataaggatg taacaaacgc tactgaccaa aatataagca acccaaacat tctaaatgaa
     2581 tcaaatagc
//
LOCUS       DAOPLS010000100         2571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_46305_length_2571_cov_3.829889, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000100 DAOPLS010000000
VERSION     DAOPLS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46305_length_2571_cov_3.829889"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1836)
                     /locus_tag="PKV52_02745"
     CDS             complement(58..1836)
                     /locus_tag="PKV52_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPD99163.1"
                     /translation="MFNNYNRLRLRRKFRARKKAIATKAESANKNLERHIFRRAHNWQ
                     FAKRFFITWLVLVSLLAGGLLIQIRGLQAAYLVIKPTSGGVYNEGVIGNIKNVNPIFA
                     SSSADSSVSALLFASLLTYDENNQLIGDLAKEWKGDASSKVYTVTLRDDAYWHDGQKI
                     TADDVVFTYKSIQNPDTKSPLYTTWRGVKVEKVDDLTVRFTLPNAYSPFAHLLTTGLI
                     PEHVLKDVPKDQLRSSKFNTQEAIGSGPFKLKTVTVDKSTNKALSETISLVKNPQYHR
                     GPVQLDGFTIKTYPDEKALKNALNNKDVVGALVDPKDNQNDNVMRFNQTSAVMIFMNT
                     SRPLLSDVKMRQALLQATDPYKISSQLSYPTTAVRSPLLKGQIGYNDELLQQKPNPDA
                     VNTTLNELGWLWGEDEPYRKKDGKELTLQFVSENTTDYAAFSEEIQKQWSQYGIKTNV
                     VLENSDDISTTSLATKDYDILMYAINIGADPDVYVYWHSSQAKPEAKPGYNFSMYSST
                     KADQSLEDGRSRQDESLRAVKYKSFLETWRKDVPAIGLHQPKITYTTNIPVYNLNEMT
                     LNTAAERFKNVYNWQVNTTKAVQDNN"
     gene            complement(1842..2060)
                     /gene="secG"
                     /locus_tag="PKV52_02750"
     CDS             complement(1842..2060)
                     /gene="secG"
                     /locus_tag="PKV52_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPD99164.1"
                     /translation="MNSIYDYVTIGSAVLMTIFILMQTRGAGLGAGFGGSAEINSVRR
                     GSDKTLFQLTIVCATIFVLSIILGIVSA"
     gene            complement(2053..2421)
                     /locus_tag="PKV52_02755"
     CDS             complement(2053..2421)
                     /locus_tag="PKV52_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPD99165.1"
                     /translation="MRKQFILFLTRTAANYAGLAISAYAGILTINGGVRTYIIGALLL
                     AVLNAIVKPLLIIISLPLIAVTVGLFLIIINGVVLYILSFLYLPLEINNFWWAMVAGI
                     VIGLVNYIVTITYERLVSDE"
     gene            complement(2442..>2571)
                     /locus_tag="PKV52_02760"
     CDS             complement(2442..>2571)
                     /locus_tag="PKV52_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016133082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPD99166.1"
                     /translation="SLSLSEGMVITIEPGLHSRKHSIGIRIEDDVVITKNGAKILE"
BASE COUNT          716 a          530 c          480 g          845 t
ORIGIN      
        1 gccttttact atactcaata ataccataag cgctaggtaa ggataaggct ttcttattta
       61 attattgtct tgtaccgctt tggttgtatt gacttgccag ttgtatacat tcttaaaccg
      121 ttctgcggca gtattcagtg tcatttcatt aagattatac actgggatat ttgtcgtata
      181 tgtaattttt ggttgatgca agccaattgc aggtacgtct ttgcgccatg tttctaaaaa
      241 tgatttatac tttacagccc gaagtgattc gtcttgccta gaacgaccat cttctagtga
      301 ttgatctgct tttgtagatg aatacataga aaaattatag cctggtttcg cttctggttt
      361 cgcttgcgaa gagtgccaat acacgtacac gtctggatcg gcaccaatat taattgcata
      421 cataagtata tcgtagtctt ttgtggctaa actcgttgta ctaatatcat ctgagttttc
      481 taatacaacg ttcgtcttga taccatactg actccattgt ttttgtatct cttcagaaaa
      541 cgccgcataa tctgtcgtgt tttctgacac aaattgtagt gttagttctt tgccgtcttt
      601 cttcctataa ggttcatctt caccccacag ccagccgagc tcgttgaggg ttgtatttac
      661 tgcgtctgga ttgggttttt gttgcaataa ttcatcgtta tagcctattt ggcctttaag
      721 tagtggcgag cgaacggcag ttgtaggata cgaaagctgc gaagaaattt tatacggatc
      781 tgttgcttgc agcaacgctt gacgcatctt aacatctgac aacaaaggtc ggctggtgtt
      841 cataaagatc atgacggcac tcgtttggtt aaaacgcatt acattatcgt tttgattatc
      901 ctttggatct acgagcgccc caactacatc tttgttattt aaggcatttt ttagggcttt
      961 ttcatctgga tacgttttaa tagtaaagcc atctagttga actggcccac gatgatactg
     1021 cggattctta acgagtgata ttgtttctga tagggcctta ttggtacttt tatcaaccgt
     1081 aactgttttt agcttaaacg gaccactccc aatcgcttct tgtgtattaa acttgctact
     1141 acgtagttgg tcttttggga catccttaag aacgtgctct ggtattaagc cggttgtgag
     1201 caagtgtgcg aatgggctat aggcattggg caatgtaaac cgaacagtaa ggtcatctac
     1261 cttctctact ttgacaccgc gccatgtggt ataaagtggt gattttgtat ctggattctg
     1321 aattgattta tacgtaaata ccacgtcgtc tgcagttatt ttttgtccgt catgccagta
     1381 ggcatcatca cgaagcgtaa cggtatatac cttagagctt gcgtcacctt tccattcttt
     1441 tgctaaatca ccaatcaact ggttattttc atcgtaagtc aataacgagg caaataataa
     1501 tgccgatacc gaactgtctg cagatgaact tgcaaaaata ggattaacgt tttttatgtt
     1561 accaattacc ccctcattat agacgcctcc agaagtaggt ttaatcacta aatatgctgc
     1621 ttgcaaacct cgaatttgaa taagtaagcc gccagctagt agcgatacga gcactaacca
     1681 agtaatgaaa aaacgtttag caaattgcca gttatgagca cgcctaaata tatgacgttc
     1741 tagatttttg ttagccgatt ctgctttagt agcgatagct tttttgcgcg ccctaaattt
     1801 gcgtcgcaag cgaagcctat tatagttgtt aaacatacct gctatgcgct cactattcca
     1861 agaataattg acaacacaaa aatagttgcg cacacaattg ttaattgaaa tagtgttttg
     1921 tctgatccac gtcgtactga atttatctct gcgctaccac caaaacctgc gccaagtcct
     1981 gcgcctcttg tttgcattag tataaatatt gtcatgagca ccgcactacc tatcgtcacg
     2041 taatcataga tactattcat cgctcactaa cctttcatat gtaattgtca ctatatagtt
     2101 taccaagcct atcacaattc ctgcaaccat agcccaccaa aaattattaa tttctagggg
     2161 tagatacaaa aaacttaata tatataacac aacaccatta ataataatta aaaataaccc
     2221 tactgtgaca gcaataagtg gtaaagaaat aattatgagt agtggcttta caattgcatt
     2281 cagcactgca aggagcaaag cacctatgat ataggtacgt acacctccat taatcgtcaa
     2341 tatacccgcg tatgcgctaa tagctaagcc ggcgtaattg gctgctgtac gagtaagaaa
     2401 tagaatgaat tgttttcgca tagttctggt atagtatagc gctactccag tatttttgcg
     2461 ccatttttcg ttattactac atcatcttct atacggatac caatgctatg cttcctactg
     2521 tgaagccctg gttctattgt tatgaccata ccttcagata aagacagcga a
//
LOCUS       DAOPLS010000101         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_46541_length_2562_cov_3.435580, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000101 DAOPLS010000000
VERSION     DAOPLS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46541_length_2562_cov_3.435580"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..528
                     /locus_tag="PKV52_02765"
     tRNA            452..528
                     /locus_tag="PKV52_02765"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:486..488,aa:Lys,seq:ttt)
     gene            1289..>2562
                     /locus_tag="PKV52_02770"
     CDS             1289..>2562
                     /locus_tag="PKV52_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPD99167.1"
                     /translation="MNLRRTTYIAIACATTLIGLFLAKYITPIPGDWFFVSVVLLPLY
                     FSSKTRVIFVGTTFLILGLWRGGVLQADYQQFATHYGEKVTITGYAIEDATYSYNGQL
                     ETTLQLTTVNGQPASGKVTLRGYTEPALYRNDNIEATGKLQSTLGGKQGRMSYADLNV
                     VSRSNSIIEKVRKNFIVGMQNALPEPAASLGTGILVGQRSLLPDDVSSALQIAGLTHI
                     VAVSGYNLTIIINAVKRLSRRLSRFQTVGISAALIYVFLLITGFSPSIVRASLVAGLG
                     MAAWYYGREFRPIVLILFAAAVTGFVNPYYVWGDIGWYLSFLAFFGVLVVAPLLVKLF
                     AKGKTELPLLPTVAIESFSAQVMTLPLSMFVFAKISSIGFIANILIVPLVPFAMLASF
                     VAGLSGMFMSLYAGWVALPARVLLNTMIHTSE"
BASE COUNT          723 a          479 c          505 g          855 t
ORIGIN      
        1 tagccttcca gccttctacc cctgcttttt caaatatctt ccacattgct acaatttgaa
       61 tgactgcaat ggctagctat aagattaccc acaccaagag agctcccgct agtatcccag
      121 cgccagcagc atctgatgtt gttaacgtat aatcatagtt atacgtatct acttgtgcaa
      181 aaaacatata tcctcctctt ttaatattag tattatacgc ctagcgccat gtaaatagac
      241 agtttacatt ttgtaattga tatgcaataa ttacccctgt tgttttttaa cactcaacat
      301 atctacaaca ggtctgtagc tcagctggtt accttgcgat aaaaacttgt tttcaatcga
      361 gaagttcagg ggtcgcagaa tgtaccttgc ctcttccaag ctccgctcgc tataaagatt
      421 gaatagcact tttagatata atagatacta tgggccagta gctcagctgg ttagagcacc
      481 tgccttttaa gcagggtgtc gtgggttcga gccccactgg tccctccagt tatattaccc
      541 cagcttagct gggtttttaa tttgattatt catcgcgtat gcacgaacaa aaaacgtcag
      601 cataatgtta tgacgaataa ctcttggcaa ttattgccag agagtaaagc tttactatat
      661 tactgaaacc gtagaatatt gcattacaca ttctttctag tactgtgaac ctgctactat
      721 taattcatga agcctaacgc aaaattcttt aacaagaaac aaatggtatt ccaagagttg
      781 tctattggaa tactaattta tatggcagta cttggctttt ataacgacta tactaccgta
      841 gtagatgcta aaagtttttc gactataatg ttagcttcca tcgttctaga aatcatgaca
      901 tttgcaacct ttttgttaaa aaataaaata gtaaaaagat taaaacgaca ttcacaatta
      961 ttttataaga tacttcggtt ttttacggtt tggttcataa tgtttgtgtc taagttcgtt
     1021 tttgtatggg caataaattg gttattcggt gaatatatat caattaacgg cttctttggc
     1081 atattgctag tagttttaag cgtgacaata atgcacagat tagggtacct tatttttcat
     1141 tacctaggca gataaaagcc atttaatttt gtgttgaagt aataataaaa atacattaac
     1201 aaactaacat gtttgatata ctcatacata ttataaaaac cgtgcatagt aattggcact
     1261 cgtctataag caaaattact caaacatcat gaacctgcgc cgaacaacat atattgctat
     1321 agcttgtgct acaacactta ttggcttgtt tctagctaaa tacataacac caataccggg
     1381 agattggttt tttgtatcgg tggttttgct tccgttgtat ttttcttcaa aaacaagagt
     1441 tatttttgta ggtactacat ttttaatcct cggtctttgg cgtggtggcg tattacaggc
     1501 agactaccag cagtttgcta cgcactatgg tgaaaaagtt actatcactg gctatgctat
     1561 tgaagatgcc acctatagtt acaacggcca attagaaact acgcttcaac tgacaaccgt
     1621 taatgggcaa ccagcaagtg gtaaagtgac acttcgtgga tatactgaac cagccttgta
     1681 tcgtaatgat aatatcgagg caaccggtaa attacaatct acacttgggg gtaagcaagg
     1741 gagaatgtca tatgcagatc ttaacgttgt ttctagaagt aattctatta tagagaaggt
     1801 tcgtaaaaat tttatcgttg gtatgcagaa cgcactacct gaaccagccg ccagcttggg
     1861 cacagggatt cttgtaggcc agcgtagttt attgcccgat gatgtcagta gcgcgttaca
     1921 aatagctggt cttacgcata ttgttgctgt gtctggctat aatttaacaa ttattattaa
     1981 tgcggtaaaa aggctttcaa gacgtttgtc gcggtttcag acggtaggca tatcagcagc
     2041 attaatatat gtatttttgc ttattacggg tttttcaccg tctattgttc gggcgagtct
     2101 tgtggcggga ttaggcatgg cggcttggta ctatggtagg gaatttagac caatagtact
     2161 tattttattt gcagcagctg taacaggctt tgttaacccg tattatgtgt ggggtgatat
     2221 cggctggtat ttatcgtttt tggcattttt tggtgtatta gtagtggcac cgctcttagt
     2281 caagttgttt gcaaagggca agaccgaact gccactattg ccgacggtag ccatagaaag
     2341 cttttctgca caagtaatga cattgccgct aagcatgttt gtattcgcca aaatttctag
     2401 cattggcttt attgctaata tattaattgt gccgcttgtg ccgtttgcta tgcttgctag
     2461 ctttgttgcc ggtttgagcg gtatgtttat gtctctatat gctggttggg tagctttacc
     2521 tgcaagagtg ctgcttaata ccatgattca tacttctgaa tg
//
LOCUS       DAOPLS010000102         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_46546_length_2562_cov_3.137615, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000102 DAOPLS010000000
VERSION     DAOPLS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46546_length_2562_cov_3.137615"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKV52_02775"
     CDS             <1..366
                     /locus_tag="PKV52_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99168.1"
                     /translation="DLTVNGHIITAGNAPVATAGTAAGTEDTQNNIPAPQVTIEGNDT
                     AGTITIVAGANTTTGDLAEVTFNQAFSKVPKVILTAGNEQTTDLKFFRSAQTGKFLIN
                     LKNAPQAGQTYTFDYFVIE"
     gene            654..1217
                     /locus_tag="PKV52_02780"
     CDS             654..1217
                     /locus_tag="PKV52_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99169.1"
                     /translation="MAKNETTSAIVDGVMKAVVVTGSISMVLLAPNILKLIDPATRKF
                     LDSLDDRAREREMNRCLRYALREKLITENYQHGITISKKGKKRLVQRDYDTLVIDKPK
                     TWDGKWRLVLFDIPAIHNKNRAQFTKKLRLLGFQPLQQSIWIIPYACKTEVHFVADSL
                     NISKYITYIKTSHIDHEESLKDRFSIQ"
     gene            1889..>2562
                     /locus_tag="PKV52_02785"
     CDS             1889..>2562
                     /locus_tag="PKV52_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99170.1"
                     /translation="MSGKAAPYHKTKKKALHVVLVLLFVATSVISSIIYLYTPSSRVQ
                     AATNSTINFQARILTNTGALVPDGYYNVEFKLYTASSGGSTVWTETYYDSNGPTAGND
                     NRIQVKNGYVTANLGTLSTFANTIDWSQELWLSMNIGGVAHTATPTWDGEMAPRMKVT
                     AVPYAFRAAEATQLKTVNGANVSTLSILAPTGGNQVFQIQDQGAGGTYNLCIQNSSAC
                     GFALLAG"
BASE COUNT          900 a          559 c          472 g          631 t
ORIGIN      
        1 gacctaacag ttaacggcca cataataaca gctggcaacg ccccagtagc tacagcaggt
       61 acagcagctg gtacagaaga cacccaaaac aacataccag ccccacaagt aaccatagaa
      121 ggcaacgaca ccgccggcac cataactata gttgctggtg ccaacaccac aaccggcgac
      181 ttagccgaag ttacctttaa ccaagcgttc agcaaagtac ctaaagttat attaacggcc
      241 ggtaacgaac aaacaactga cctcaaattc tttagaagtg cccaaacagg caagttctta
      301 attaacctta aaaatgctcc acaagccggc caaacctaca ccttcgacta cttcgtcatc
      361 gaataatcca ccccccacac ctcaaaccct atagccacgc accccctcaa ccacccaacc
      421 aacccctata accacgcgcc ccttaaacca cacagccgaa caccacccga acccatccac
      481 caccgaacac cccggaaccc aacccaccca acccctcgaa aaatgtaaat aaatcacaca
      541 cctattcaat ttatttatat aaagaagctg aataagttac acatcaaaat caataacaga
      601 tgagaaatac cataagtatt gttaaataaa ctaaacaggt gttaaattta tttatggcta
      661 aaaatgaaac aacaagtgca attgtagacg gggtaatgaa ggcggttgta gtaactggat
      721 ctatttctat ggtgttactt gcgcctaaca tcttaaaatt gattgacccg gctactcgta
      781 aatttcttga tagtcttgac gatcgtgcac gagaaagaga aatgaataga tgtttgcggt
      841 atgctctcag ggaaaaactc attacagaga actaccaaca cggcataacc ataagcaaaa
      901 aaggtaaaaa gagactggtc cagagagatt acgacaccct agtaattgat aaaccaaaga
      961 catgggacgg taaatggaga ttagtattgt ttgacattcc agctattcac aataaaaatc
     1021 gtgctcaatt caccaaaaaa ctgcgattgc tcgggttcca accactgcaa cagagcattt
     1081 ggattattcc ttatgcttgt aaaacagaag tgcattttgt tgcagatagc ctcaatattt
     1141 ctaagtatat tacatatatt aagacctctc acatagacca cgaagaatcg ctgaaagaca
     1201 gattttccat acagtaattc ataaataaga taaacacctg ttaattttat ttatgaggaa
     1261 ttttgttctg gggttgggca tgtgaagggg gatcagatag gtaattagaa gatgaaaaat
     1321 caaaccatcg gtcacaccat gccacaggat ataaaatatt agctgtggaa aagtcagaaa
     1381 ttagtaatta tataatagta ataattacta caataaagtg tagtaaaaga gtatacagta
     1441 tataaagcaa ctgtgctcta tatacaataa ttatcagtaa tttatacaat ctaataacag
     1501 ataaaatatg ctactttatt gtctgattac gcgcagtttt attattttta tatgttataa
     1561 aaaaaactac gtatttatag tactatttta tacatttaca tgtattttag gtgaacaaaa
     1621 aattaggtgt aatgtgggtt gtattagttt cacggatgcg gtaacctgta ctcagagttt
     1681 gtcaaaagaa caaagcactg tttacaaaac aaaagctaaa aacaaagtcc aaaagaactt
     1741 aaaataaaag ctaaaacgta aacaaataga caagccgaca aaagcatttg ccgcacaaat
     1801 gctaaaacaa atttaaaaaa acaaatccgc agtgaataga ctaatccgca ctagtcgaat
     1861 ctttaactgc acccgtcgcc tctttgtatt gagtggtaag gccgcaccat accacaaaac
     1921 aaaaaagaag gcattacacg tggtgcttgt tttgctattt gtagctacct ctgttatttc
     1981 tagtattata tatctatata cgcctagcag ccgtgtacag gcggctacaa acagcacaat
     2041 aaactttcag gctcggatac tcacaaacac tggtgcatta gtgcctgatg ggtactacaa
     2101 cgttgagttt aaactgtaca ctgccagcag tggtggttct actgtgtgga cagaaactta
     2161 ctacgacagc aatgggccaa ctgctggtaa cgataaccgt atacaggtta aaaacggtta
     2221 cgtaacagcc aaccttggca cgctaagtac atttgccaac accattgatt ggagccaaga
     2281 gctatggctt agcatgaata ttggtggtgt tgcccatacg gctacgccaa cgtgggatgg
     2341 cgaaatggca ccgcgcatga aggttactgc tgtgccgtat gcgttccgtg cagcagaagc
     2401 cacccagcta aaaacagtca atggtgcaaa tgtgagcaca ttaagtattt tggcaccgac
     2461 tggcggaaac caggtgttcc agatccaaga ccaaggtgct ggtggaacgt ataacttgtg
     2521 tattcaaaac agcagtgcct gtggttttgc gctacttgca gg
//
LOCUS       DAOPLS010000103         2559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_46599_length_2559_cov_4.945288, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000103 DAOPLS010000000
VERSION     DAOPLS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46599_length_2559_cov_4.945288"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1629
                     /gene="gatB"
                     /locus_tag="PKV52_02790"
     CDS             166..1629
                     /gene="gatB"
                     /locus_tag="PKV52_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPD99171.1"
                     /translation="MDKKYLVQNRYYPTIGIECHVQLKTKTKLFAGVDNDAREASPNT
                     LISPLCIGMPGTLPVLNAAVIPLSIRAGLALRATISPTSHFDRKHYFYPDLPKGYQIT
                     QYERPIVVDGTVEAPMLDGSYVTVGIERAHLEEDAGKSSHPTGETYSLVDLNRAGTPL
                     LEIVSAPDMHSAEEARAYVHELYLLMKYADVSDVDLYHGNMRFDVNVSVSTDPKKLGT
                     RSETKNLNSFKSVEKAVEYEIKRQIRLLEKGQSIVQETRGWDDDTQQTVSQRSKEDAH
                     DYRYFPDPDIPPITVSQEAISYEAQTMPLLPAALREQFGELGLDTTQISTIIELPIIA
                     NIVRVTIEQHDTLTAKTIAHWCVGEITKLVADNKMTWLQVQSAVPLLVALASMQNDNK
                     VSSSAAKGILTEMITTNTEPMEVAKKHDLLQVSDEGELVLVVERVLAENQKAALDVRN
                     GELKAIGFLVGQVMKASAGKANPAIVQQLIKKQLGVK"
     gene            1631..2509
                     /locus_tag="PKV52_02795"
     CDS             1631..2509
                     /locus_tag="PKV52_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD99172.1"
                     /translation="MKKPTKAIIAAAGLGTRFLPQTKAMPKEMLPIIDKPVIQIIVEQ
                     LVASGVTDIIIVTGSTKRAIEDHFDRSDELENELRHKGKIKQADEIKKIAELANFIYV
                     RQKGTPKGNARPLLNASHLLDDDEPFFMFFADDFFRSKIPQATQLLKVYEATGASVIS
                     LITVNDADTSKYGIADIVGEQSKGYAQVKRLVEKPGPHAAPSRFAAVSGYLLTPDILP
                     VVAQENVGHSGEIVLADSINELAQQGKVYGKFIEGIYHDAGDKASYLRAIVDHALADK
                     TLGKEFRDYLVARLNK"
BASE COUNT          854 a          508 c          573 g          624 t
ORIGIN      
        1 tgatagctgg caaaaatgca ctacggcacc gtgatgatat atggacagca cataagttac
       61 gaaacaaaat tgtacacgaa accgcatttg agccgagtga aaagcaagtg aagtctgcat
      121 taagtgcata ccacaagaca tttaaagact taggggtttg gtaacatgga caagaaatac
      181 ctcgtacaaa atcgctacta tcctacgata gggatagagt gtcatgtgca attaaaaaca
      241 aagacgaagc tatttgctgg tgtagacaac gatgcccgtg aggcttcgcc taatacactc
      301 attagccctt tgtgtattgg tatgccgggt acattaccgg tactaaatgc agcagtgata
      361 ccgttgtcta ttagggcagg cttagctcta cgcgctacta tttcgccaac cagtcacttt
      421 gacagaaaac attattttta cccagatttg ccaaaaggtt atcaaattac acaatatgaa
      481 cgacctatag tagtagacgg cacggtagaa gcgccaatgc tagatggctc gtatgtcacg
      541 gtaggtatcg agcgggcaca cttagaagaa gatgcaggta aaagcagcca tccaactggc
      601 gaaacctaca gtctggtaga tcttaatcgc gctggtaccc cacttctaga aattgtatcg
      661 gcacctgata tgcattctgc tgaggaagct cgtgcgtatg tgcatgaatt gtatcttctg
      721 atgaaatacg ctgatgtttc cgatgtagac ctatatcatg gcaacatgcg ttttgatgtg
      781 aacgtaagtg ttagcactga tccaaaaaaa ttaggcacca gaagtgaaac gaaaaactta
      841 aatagtttta agagtgtaga aaaagcagtt gagtacgaaa taaaacggca aatacgctta
      901 ctagaaaaag gccagtctat tgtacaagaa acacgtggat gggatgatga tacacaacaa
      961 acggtatctc agcggagcaa agaagatgca catgactata ggtattttcc tgatcctgat
     1021 attccaccca ttacagtcag ccaagaggca attagctatg aagctcaaac tatgccgtta
     1081 ctacctgctg cactccgcga acaatttggt gaacttggtt tagatacgac acaaatttca
     1141 accattatag aattaccaat cattgccaat attgttcgag tgacaattga gcagcacgat
     1201 acactcacag cgaaaactat tgcccactgg tgtgtcggcg aaataaccaa gctcgtggca
     1261 gacaataaaa tgacatggct acaagtgcaa tcggctgtac ccctgcttgt tgcattggct
     1321 tctatgcaaa atgacaacaa agtgagcagc agtgccgcta agggcatact aacagagatg
     1381 attacaacca atacagaacc tatggaagtt gctaaaaaac atgatttgtt gcaagtgtct
     1441 gacgaaggag agctcgtatt agttgttgaa cgagtgctcg cagagaacca aaaagctgca
     1501 ctagatgtac gaaatggcga acttaaggca ataggctttt tggtgggcca agtaatgaaa
     1561 gcgagtgctg gcaaagccaa cccagcaatt gtgcagcagc tgattaaaaa gcaactcgga
     1621 gtaaagtaac atgaaaaagc caaccaaagc tattattgcg gctgcaggct tggggacacg
     1681 tttcttaccg caaaccaaag caatgccaaa agaaatgctt cccatcatag acaagccagt
     1741 catacaaata atcgtagaac agctggtagc atctggtgta acagatataa ttattgtgac
     1801 aggtagtaca aaacgagcga tcgaagacca ttttgataga agcgatgagc ttgaaaacga
     1861 gctccgccat aaaggtaaaa ttaaacaggc tgacgaaatt aaaaaaattg cagagttagc
     1921 aaattttatt tatgtgcgcc aaaagggaac gccaaaaggg aacgctcggc cgcttttaaa
     1981 tgcatcccat ttacttgatg acgatgaacc attttttatg ttttttgccg acgatttttt
     2041 tagaagcaaa ataccacaag ctactcagct gctaaaggta tatgaagcca caggagcgtc
     2101 tgtcatctcg cttattactg tcaatgatgc agataccagt aaatacggta tcgcagatat
     2161 agttggcgag caatcaaagg gctatgccca agtaaaaagg ctcgtagaaa agcctggccc
     2221 acatgcagca ccatcaagat ttgcggcagt gagtggctat ttactaactc ctgatattct
     2281 gccagtggta gcacaagaaa acgttggaca cagcggagaa atagtacttg ccgatagtat
     2341 taatgagctt gcccaacagg gtaaggtgta tggtaaattc atagaaggta tttatcacga
     2401 cgcaggtgac aaagccagct acttaagggc aattgtagat cacgccctcg cagataaaac
     2461 gcttggaaaa gagtttagag attatttggt cgctcgactt aacaagtaat gtgtgtaaaa
     2521 tatagtatat ggatgcattt gaaattttag tcattatac
//
LOCUS       DAOPLS010000104         2552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_46785_length_2552_cov_3.869043, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000104 DAOPLS010000000
VERSION     DAOPLS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46785_length_2552_cov_3.869043"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKV52_02800"
     CDS             <1..601
                     /locus_tag="PKV52_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPD99173.1"
                     /translation="LRRISMAKQSEVYMFDEHTKNMWWVLVLTGLLSVIFGFVAIIWP
                     GITIGVLALLLAVYIGITGIADIVSGIKKFSTGFFAAALTLLVGLLFLGVSVYLLSNI
                     GSGLAVMTLVLLIALSFIVRGVVAIVVAFADPDYRATRWLNVVVGSLSVLAGLVVAWY
                     PSAATLAWVWVIGFFALVSGAMQIGLGFTAKDHLEKGKK"
     gene            784..1854
                     /locus_tag="PKV52_02805"
     CDS             784..1854
                     /locus_tag="PKV52_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPD99174.1"
                     /translation="MDVITIVLIVLTIVLLATIMYLVYALRRSAAPAITNNEIQNLNN
                     RLDAIKDSLNITVNDRLDKNTKLMSDTMQQQFSQSAKLIADVTNRLTKLDETNKRVVD
                     VADELKMLQNVLQNPKQRGGIGEYYLDVVLGNVLPSKLYQLQYKFADGEIVDAVIKLD
                     KGRLLAIDSKFSLENYNRLVEETDKTKRVALAKLFKADLKNRIDETAKYIRPAENTLD
                     YAFMFIPSEAIYYDLLINQVGMTNTSARDLLEYAFIDKKVIIVSPTTLLAYLQTVLQG
                     LRSLQIEEQAKEIQKRVGELGKHIATHETYMQKLGTSLGTTVNHFNTAHKELKKMDKD
                     VIKIAHTDASVEPLVIEKPKED"
     gene            complement(1875..>2552)
                     /locus_tag="PKV52_02810"
     CDS             complement(1875..>2552)
                     /locus_tag="PKV52_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPD99175.1"
                     /translation="MRNIDALMAWDAILRLRRKHYDIDLILHRSLFAIEDVGFNAIFI
                     RNNQILRDIAKTLYRKLPKELDANMQQSEIAIESLWDETYSIYFSRDFITKKLLRQPT
                     ISSLLPLYSGAISKKRAETLVKILTTSSAFWTQFPVPSVPKNSRIFDEWRYWQGPSWL
                     NTNWLIIDGLERYGFTQESQALVSHTLQMVRNSGVWEYYNPNNGEGLGSADFTWTAAL
                     TLDLLHR"
BASE COUNT          780 a          469 c          548 g          755 t
ORIGIN      
        1 tttaaggagg atctctatgg ctaagcagtc agaggtgtat atgttcgatg aacatacgaa
       61 aaacatgtgg tgggtgttag tcctaacagg gctattatca gtcatctttg gttttgtggc
      121 tattatatgg ccgggtatta ccattggtgt tctagcattg ctacttgcag tgtatatagg
      181 aattacaggt atagctgata ttgtcagtgg cattaaaaag tttagcacgg gcttctttgc
      241 tgccgcgctt acactattgg ttgggctttt gttcttgggc gtatctgtct acctactaag
      301 taacattggt tctggcttag ctgtcatgac actcgtacta ctcattgcgc taagttttat
      361 tgtgcgtggt gttgttgcaa tcgtcgtagc atttgcagat cctgactatc gggctacgcg
      421 ctggcttaat gtcgttgttg ggtcattatc ggtacttgct ggtttagtag ttgcttggta
      481 tccgagtgca gcgacattgg catgggtatg ggtaattggt ttctttgccc tcgtaagtgg
      541 cgcaatgcaa attggacttg gctttactgc taaagatcat ctagaaaaag gtaaaaaata
      601 aatagagtat agttgctata aacgtgctgt agcgctacac taaaagtgga agttaaactt
      661 ccgccaaagc cgggtgaaac atcccggctt ttttaatggg tgcattgcat gtaccataga
      721 aggtatgacc ataagcgcgc acagattgat aatagataac agcactttat acgcaggttt
      781 tacatggatg taattaccat tgttctgata gttctgacaa tagttttgtt ggcaaccatt
      841 atgtatttag tgtatgcgct cagacgtagc gctgcccctg ccataaccaa caatgagata
      901 caaaacttaa ataatcgcct cgatgcaatt aaagacagtc ttaatatcac tgttaacgac
      961 cgcttagaca aaaacactaa gcttatgagt gatactatgc aacagcagtt tagccaaagt
     1021 gcaaagctaa ttgctgacgt caccaataga cttacaaaat tagacgaaac gaataagcgt
     1081 gtggttgatg ttgcagatga acttaaaatg ctgcaaaatg tgctccaaaa ccctaaacaa
     1141 cgtggtggta taggtgagta ttatttagac gtggttcttg gaaatgtttt accaagtaag
     1201 ctatatcaat tacagtacaa atttgctgac ggagaaatag tcgatgcagt gattaaacta
     1261 gataaaggca gacttctggc aattgatagt aaatttagcc ttgaaaacta taaccgatta
     1321 gtagaagaaa ccgataaaac taaacgtgtt gcactcgcaa aattatttaa agcggattta
     1381 aaaaatagga tagacgaaac agctaagtac atacggccag cagaaaacac gctagattac
     1441 gcatttatgt ttataccgag cgaagccatt tattatgatc tacttattaa tcaggtaggt
     1501 atgaccaata cgtctgcgag agatttacta gaatatgcat ttatcgataa aaaagttatt
     1561 attgtcagcc cgacgacatt actcgcatac ttgcaaacag tattgcaggg cttgcggagc
     1621 ttgcagatag aggagcaagc caaagaaatt caaaaaaggg taggcgagct tggtaaacat
     1681 attgcgacac acgaaacata catgcaaaag cttggcacaa gtttagggac aaccgtaaat
     1741 cattttaaca ctgcgcataa agagcttaaa aagatggata aagacgttat aaaaatagct
     1801 cacacggacg caagtgttga accacttgtt attgaaaagc ccaaagaaga ctagcttagc
     1861 ctatttagta gcgtttatct atgtaataaa tctagcgtaa gcgctgctgt ccaggtaaag
     1921 tcagcactgc ctaagccctc accgttgttt ggattataat attcccagac gccactgttg
     1981 cgcaccattt gtaatgtatg gctgacaagc gcttgagatt cttgtgtgaa accataacgc
     2041 tctaggccat ctatgatgag ccagtttgta ttgagccaac ttggtccttg ccagtaccgc
     2101 cactcgtcaa atattcgact atttttaggc acagacggta ccggaaattg cgtccaaaag
     2161 gcagaagaag tggttaatat ttttacaagt gtttccgctc tttttttgct aatagcacca
     2221 ctatacagtg gcaacagact agatattgtt ggctgccgaa gcagtttttt ggttataaaa
     2281 tcacgactaa aataaatact gtaagtttcg tcccacagag attctatggc aatttcgctt
     2341 tgttgcatgt tagcatctag ttctttaggc agttttctat ataaagtctt agcaatatct
     2401 cttagtattt ggttattgcg aataaaaatg gcattaaagc ctacatcttc tatagcaaat
     2461 aacgatctgt gcagaataag gtctatatca taatgtttac ggcgtaagcg taatatagca
     2521 tcccatgcca tgagtgcatc aatattccgc at
//
LOCUS       DAOPLS010000105         2532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_47289_length_2532_cov_4.291078, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000105 DAOPLS010000000
VERSION     DAOPLS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47289_length_2532_cov_4.291078"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PKV52_02815"
     CDS             complement(<1..438)
                     /locus_tag="PKV52_02815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD99176.1"
                     /translation="MANNSNYPKPEIIQTRAEYVTQSTWHIVSNIAYLAMREAINHEM
                     RTDEEIAYMTGWSDRRRPTYYDSMGDPNSQVGSTLRAHQQYWNTRLTMAVKDGTPVGY
                     AYSADNASGSWPIRQLKHRDPLQKKNYLWIRGVAVVPEEQGRGI"
     gene            complement(471..1115)
                     /locus_tag="PKV52_02820"
     CDS             complement(471..1115)
                     /locus_tag="PKV52_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HPD99177.1"
                     /translation="MAKLYLSSYRLPTPDDLIELIGKPAGEISVAIIPNAKDYYIPRA
                     KAVRISKLEQYYNELGLKTSVIDLLATPSQQLKAQLLTCDAVHVAGGNTFCLRYASKK
                     ANFDSIVESLLRKGIVYIGESAGAILAGISLKSVELDDEPLFAEEIIYEGLGLVPYFI
                     LPHADNPSFIKSNQLAKQTYQPQDMIVLNDNQAAIFDGATLPRIVTAHTLPNAL"
     gene            complement(1158..2270)
                     /locus_tag="PKV52_02825"
     CDS             complement(1158..2270)
                     /locus_tag="PKV52_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPD99178.1"
                     /translation="MKTAQALNYTKKLISISSDSGNEQAISSYIQETVATLGSVQTIE
                     QSVVLHIVGKDQTKALIFNGHIDTVPAGSSWQTQPLTAVEKNGKLYGLGASDMKSGVG
                     IMLQLANDFANQQPPCDMWFMFVEQEETDGRGTKKVLDVLPNFTNAYPGGVEGFILEP
                     TNSTYVAIGHKGNIFVTLTFDGQGGHGSMELPYQQRASHKAAHFIQQLPRIAQSWKTT
                     YTHAILGEPTINVTNIESVGSKALNAVPQSVLVSLDIRTTPKLSAQFAAAIDALATTY
                     GATYSYPYKPNGYGLCSEDSQLLAIAKKQFAVKDIRVFQGASDQLFFTANNIPMLIYG
                     PGSNAVMHQPNEYVEISAIDRTYDVLQSFIAHFTDQ"
     gene            complement(2280..>2532)
                     /locus_tag="PKV52_02830"
     CDS             complement(2280..>2532)
                     /locus_tag="PKV52_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99179.1"
                     /translation="SYHDEHIGTTLAWLATESALHEGKTALAFCNTASLNTFIRIGYS
                     EAPLHEVPQAALAECAGCKNCDFDKLKQPCADTIMIKNP"
BASE COUNT          632 a          531 c          558 g          811 t
ORIGIN      
        1 tattcccctg ccttgttctt ctggcacgac ggcaacacca cgtatccata aatagttctt
       61 tttttgtaaa ggatctcgat gttttaactg acggataggc cagctaccag atgcattatc
      121 tgcagaatat gcatagccga caggcgtgcc atccttgact gccattgtaa gccgagtatt
      181 ccaatattgt tggtgagcac gtaatgtgct accaacttgc gaattagggt ctcccataga
      241 atcgtaatag gtcggacgac gtctatcgct ccaccctgtc atgtaggcaa tctcttcatc
      301 tgtacgcatt tcatgattta tagcttcgcg cattgctagg taggcaatgt tgcttactat
      361 atgccaggtt gattgtgtta cgtattctgc tcgggtttga ataatttcag gtttggggta
      421 gtttgagttg tttgccatag ttacataagt ttacaattgt gcacgtgttt ttacaacgca
      481 ttgggtagag tatgagccgt aacgattcgc ggtaatgtag caccatcaaa gatagcagcc
      541 tgattatcgt ttagtactat catgtcttgt ggctgatacg tttgcttggc aagttggttg
      601 gattttataa aactaggatt atcggcatgc ggcaaaataa aatacggcac taaaccgaga
      661 ccttcataga ttatttcttc tgcaaataag ggctcgtcgt ctagttctac acttttaagt
      721 gaaatgcccg ccaatattgc cccggcgctt tctccaatat atacaattcc cttgcgtagt
      781 agtgattcaa caatactgtc aaaattggct tttttgctag cgtagcgcag acaaaacgta
      841 ttgccaccag caacgtgcac tgcatcgcaa gtgagcaatt gtgctttaag ctgctgtgat
      901 ggagtagcta gtaaatcaat tacgcttgtt tttagcccaa gctcattgta gtactgttcg
      961 agtttgctaa tacgcacagc cttagcacgc ggtatgtaat aatctttggc attcgggata
     1021 atcgcaactg atatttcacc tgctggctta ccgattaatt ctatcaaatc atcaggagtt
     1081 ggtaggcgat atgaacttaa atagagtttt gccataccgt tattgtacca actgttgttt
     1141 ggttaatcct attgatttta ctggtcagta aaatgtgcaa tgaatgattg caacacatca
     1201 tatgttctgt ctatcgcact gatttctaca tattcattgg gttggtgcat gactgcgttg
     1261 gagccagggc catagatgag cattgggata ttgttggcag taaaaaatag ttggtcagac
     1321 gcaccctgaa agactcgaat gtcttttaca gcaaattgtt tttttgctat agcgagcaat
     1381 tgactgtctt ctgagcagag tccatacccg tttggcttat acgggtagct ataagtcgca
     1441 ccataagttg tagcaagtgc atcaattgcg gctgcgaact gtgcactcag ctttggtgtt
     1501 gttctgatat ctaagcttac gagcaccgat tgcggcacgg catttaaagc tttgctacca
     1561 accgattcta tgttcgtgac gtttatggtt ggctcaccaa gaattgcatg ggtgtaggtg
     1621 gtcttccagc tttgagcgat tcgtggcagt tgctggataa aatgggctgc tttatgactt
     1681 gctcgctgct gatatggaag ttccatagaa ccatgcccac cttgcccatc aaaagtaagt
     1741 gttacaaata tgttaccttt gtgccctatc gcgacatatg tgctgttggt tggctctaaa
     1801 ataaaacctt ctaccccgcc agggtatgca tttgtaaagt ttggcaatac gtcgagcact
     1861 tttttggtac ccctaccgtc ggtttcttct tgctctacaa acatgaacca catatcgcag
     1921 ggtggttgct ggttggcaaa atcgttagca agttgcagca taatacctac gccacttttc
     1981 atatcactgg cgcccaggcc gtacaatttg ccattttttt ctacagctgt tagtggttgg
     2041 gtttgccacg acgaaccagc gggcacagtg tctatatgac cattaaaaat aagcgctttc
     2101 gtttggtctt tacccacaat atgtagaaca acagactgct ctatagtctg cacacttccc
     2161 agtgtagcga cggtttcttg aatatacgag ctaatagctt gttcgttgcc agaatcagac
     2221 gaaatactaa tcagtttttt ggtgtaatta agggcttgtg ctgtcttcat aggtatattt
     2281 tacgggtttt ttatcattat ggtatcggcg catggttgtt tgagtttatc aaaatcacaa
     2341 tttttacaac cagcacactc ggctagtgca gcttgaggaa cttcatgtaa tggagcctca
     2401 ctatacccta ttcttataaa tgtgtttagg ctcgctgtat tacaaaacgc caatgctgtt
     2461 ttgccttcgt gtaaggcgct ttctgtagcc agccatgcca aagtagtacc tatatgttca
     2521 tcgtggtagc tg
//
LOCUS       DAOPLS010000106         2528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_47396_length_2528_cov_3.826931, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000106 DAOPLS010000000
VERSION     DAOPLS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47396_length_2528_cov_3.826931"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..913)
                     /locus_tag="PKV52_02835"
     CDS             complement(56..913)
                     /locus_tag="PKV52_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99180.1"
                     /translation="MYPDQQNQQPVAQNQYGPPAVGMPQPGAPVVGQMSASANEAATA
                     PKSNPNSTQNVLQIAEIRDGVAIMNDGSFRSVLMCRSINFDLMSPEEREGVEYAYQNF
                     LNSLYFEVQILVRSSKVDIRPYLEKLDKIRSEHDNMLLGVLMEDYINYIAALADQTNI
                     MDKKFYIAIPFYSSLDTRKVAQASKSVLTTIFGHKEQVVTIDEATLEAAKTELKNRAQ
                     TVLSGLMQCGVQGAALDTQELIELYYDAYNPDTATRQPLKNFADLTAPVVTKGQGNAA
                     QPNLDREMQ"
     gene            complement(944..2065)
                     /locus_tag="PKV52_02840"
     CDS             complement(944..2065)
                     /locus_tag="PKV52_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HPD99181.1"
                     /translation="MATYKVIQDVEAEDKILGPLSLKQLIFAAIAAGSIFIAFRIVVA
                     TGVIYTAIPFLPFILVFGVLAAPLGRDQPTEMWLAAQIRFYTKPRVRIWDQSGIKNLV
                     NITAPIRIEKIYTDGLNQNQVRSRLKALASTLDSRGWAVKNVDVNMYTTPGYAQFNSD
                     RIIDPVSLPITVSDIDVRASDDILDVANNDTAQHFDNLMQTAAQTHHQELMAKMHSAA
                     VQQPSAALPSSDADDDALNYTPPAATPPSYDTPPPQQQATIPTDQPIHEITQEDEQKI
                     IEHAKLERQRSEVQYASHHKVVLTPQQQADLAKQQALKDQEQAQEAAESQPSQQTHLS
                     KTDTIELANANLKVSTIAGLAKHKTQEKQDPTEVVVKLH"
     gene            complement(2079..>2528)
                     /locus_tag="PKV52_02845"
     CDS             complement(2079..>2528)
                     /locus_tag="PKV52_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99182.1"
                     /translation="KRSDWIKQNNEAADPRMRPAPAYDTFGAAELARYHSSGIEYTDP
                     SGATRHGDLITYLETLEQNAKAPGASTEDVEKYDEFVSRMGSGLQQMLNDPEARTAAS
                     ANATTLKGYKTYLTRHLEEVATRTHSEGATAGYLTLEDNIDSNGTIK"
BASE COUNT          559 a          535 c          596 g          838 t
ORIGIN      
        1 tcggcaagtg ccacagggtc aagctgctgc ttttttttct taaaaagacc catatttatt
       61 gcatctccct atctaggttt ggctgggcag cgttgccttg gcccttagtc actaccggcg
      121 cagttaaatc tgcaaagttt tttaggggct ggcgggtggc agtatctggg ttgtaggcgt
      181 cataataaag ctcaattagt tcttgggtat ctagtgcagc accttgtaca ccacattgca
      241 tgagcccaga aagcaccgtt tgggctcggt ttttcagttc tgttttggca gcctctaagg
      301 tggcttcgtc tatcgttacc acctgttctt tgtggccaaa tatggtcgtg agcacacttt
      361 tgctggcttg ggcaaccttt cgcgtgtcta ggctgctata aaacggaata gcaatgtaaa
      421 actttttatc cattatattg gtttggtcgg cgagcgctgc tatgtagttt atgtaatctt
      481 ccatcaaaac acctaggagc atgttgtcgt gctcggagcg tattttatct aatttttcta
      541 ggtatggccg aatgtctact ttacttgacc taacgagtat ttgaacttca aaatacagcg
      601 aatttaaaaa gttttggtag gcgtattcta cgccttcgcg ttcttctggg ctcataaggt
      661 caaaattaat gctccggcac attagtaccg acctaaacga cccatcattc ataatggcca
      721 cgccgtctct tatttctgca atttgtagca cgttttgggt actatttggg ttgcttttag
      781 gcgcagtggc ggcttcgttg gcagatgccg acatctgccc cacaacgggt gcgccaggtt
      841 gaggcatccc gacagccggc ggtccatact gattttgtgc aactggttgt tgattttgtt
      901 gatctgggta cattcttatg ggctataacg agggtcccac cccttaatga agcttcacta
      961 caacttctgt tgggtcctgc ttttcttgtg ttttatgctt ggcaagacca gcaattgtag
     1021 ataccttcag gttagcatta gcaagttcta ttgtatcagt ctttgacagg tgtgtctgct
     1081 gggagggctg gctttctgct gcctcttggg cttgttcttg gtctttaagt gcttgctgtt
     1141 ttgctaaatc tgcttgctgc tgaggtgtta ggacaacttt atgatgcgat gcatattgta
     1201 cttcagagcg ttggcgttcg agtttagcgt gttcgataat tttttgttca tcttcttggg
     1261 taatttcatg tatgggctga tctgtaggta tggtagcttg ttgttggggc ggtggtgtgt
     1321 catatgaagg cggtgtagct gctggtggcg tatagtttag ggcgtcatca tctgcatcac
     1381 tactaggcag ggctgcactt ggttgctgca ctgcagcgct gtgcattttt gccattaatt
     1441 cttgatgatg ggtctgtgct gccgtttgca tgaggttatc aaaatgctgg gcagtatcat
     1501 tgtttgctac atctaaaata tcgtcagaag cacgcacatc tatgtcgctg acagttattg
     1561 gcaagctaac cgggtctata attctatcag aattaaattg ggcgtagcca ggcgtggtat
     1621 acatatttac atctacgttc tttaccgccc aaccacggct atctagggta gaagcaagtg
     1681 cttttagccg actacgcact tgattttgat ttaagccatc ggtgtaaatt ttttctattc
     1741 ttataggtgc agtaatgttc actaagtttt taataccact ttggtcccat atacggaccc
     1801 tcggcttggt gtaaaaacgt atttgtgccg ccaaccacat ttctgttggc tggtcacgcc
     1861 caagaggtgc cgccaacacg ccaaaaacta atataaatgg caaaaacgga atagcagtgt
     1921 atataacccc agttgcaacc acaatcctaa aagcaataaa tatagaacct gcagcaatag
     1981 cggcaaagat aagctgcttt aggcttaggg gccctagtat cttatcttct gcttctacgt
     2041 cttgtattac tttatatgtc gccatacatt taccgtgttt attttatggt accatttgaa
     2101 tctatattgt cttctagggt gagatatcct gctgttgcac cttcggaatg tgtccgggtt
     2161 gcaacctctt ctaagtgtct agtaaggtat gttttatatc cttttagagt tgttgcattg
     2221 gctgacgcag ctgttcttgc ctcggggtca tttaacattt gctgtaaacc agaacccata
     2281 cggctaacaa actcatcata tttttctaca tcttccgtag atgcacctgg tgcttttgcg
     2341 ttttgttcta gagtctcaag gtatgtaatc aggtcgccat gacgggttgc accgcttgga
     2401 tcagtatatt caatacctga agagtgataa cgtgcgagtt ctgcagcacc gaaagtatcg
     2461 taagcagggg cgggtcgcat tcttgggtca gctgcttcat tattttgctt tatccaatct
     2521 gaacgttt
//
LOCUS       DAOPLS010000107         2518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_47637_length_2518_cov_3.767763, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000107 DAOPLS010000000
VERSION     DAOPLS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47637_length_2518_cov_3.767763"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            500..2344
                     /gene="thrS"
                     /locus_tag="PKV52_02850"
     CDS             500..2344
                     /gene="thrS"
                     /locus_tag="PKV52_02850"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPD99183.1"
                     /translation="MTDQKLHAMRHSLAHIMADAVGQLWPHAKFGVGPVIENGFYYDI
                     DLGNTTISEDDFSKIEKKMKEIIKSNVSFVKTEKSIQDAIVWAEQSDQPYKVELLNDL
                     KRSGTTSAKDIDTEELGLPSKGTSKVETVSFYTSGSFTDLCRGPHVESTKDIGAFKLM
                     RVAGAYWRGKENNPQMQRLYGVAFGSKQELDEYLQMLEEAKKRDHRKLGQELDLFTFS
                     DLVGPGLPLYTPNGAVLIEELRNALNEIGRQYGMRQVSIPHIAKIELYETSGHAQKFS
                     GELFHVKSHYEQDFVLKPVNCPHHTQIYAGHPRSYRDLPLRYVEQTMQYRDEKPGQLG
                     GLQRTRGFTVDDGHTFCRADQIKEEASIIVEIIRDFYESLGLWGNHWVSLSVRDYANP
                     DAYIGDAADWEVAEQMLQEVSDIHGLGAKKMEGEAAIYGPKLDFMFKDALGRETQLAT
                     IQLDFAMPKRFGLTYVNEDGKDAIPVMIHRAILGSFERFIMLLIEHYAGRFPVWLAPE
                     QVRFITVNQTTPVVQVAEKAHEKAKQLGLRTSVDNANDSVGKKIRNAEKMKIPYTIVI
                     GEKEVESGETTPRIRNDIEVQLEHPIKIDNFLKTVANEAKTRTTKTTL"
BASE COUNT          812 a          479 c          581 g          646 t
ORIGIN      
        1 ttgaacctct gacctcttcc acgtcaagga agcgctctag ccaactgagc taaggaccca
       61 actaagcgaa tggtataagg catctaattg ttaaccaagt gcattactta aaacgatact
      121 ggtatcgctt aaacactttt gacgtaagta ccacactcat tgttatgtgc gcacaatagg
      181 ttgcacataa cacttgtgag tataacaata acacctctcg gcacgcaagt atccacatct
      241 gtggataagc taacaaaatg atactagtaa aaaaaggcta gcctgtatat actactacgt
      301 acaggtgaag ttttatcagg tgtgttgacg ccgaacgatc ggctacgatg ggtacacatc
      361 tattcatcgc tagcgatgtt gtcttataac ctttaaaagg ttgctgcgag gtaagataac
      421 atcgcttttt tagtacagag gtgttagtta tggagtacaa tatacaagaa ggcaagtgaa
      481 aaggtaagga ttttacgaca tgactgatca aaaactacac gcaatgcgcc atagtttggc
      541 acacataatg gcagacgccg taggacagct ttggcctcat gctaaatttg gcgtcggacc
      601 agtaatagaa aatggttttt attatgatat cgatttaggc aatacgacaa tttctgaaga
      661 tgatttttca aaaatagaaa agaaaatgaa agagattatc aagagcaacg tttcatttgt
      721 caaaacagaa aaatctattc aagatgctat tgtgtgggcg gagcaatcag accaaccata
      781 caaggtagag ttattgaatg atcttaagcg gtctggcacg acaagtgcaa aagacataga
      841 tacagaagaa ctcggcttgc catcaaaagg taccagcaaa gtagaaacag tatcttttta
      901 cactagtggt agttttacag atttgtgccg aggtccacac gtagaaagca cgaaggacat
      961 tggtgctttt aaactgatgc gtgtagcggg cgcgtattgg agggggaaag agaataatcc
     1021 tcagatgcag cgcctctatg gggtagcatt tgggtcaaaa caagaactag acgaatattt
     1081 acaaatgctt gaagaagcga aaaaacgaga tcatcgcaaa ctcggccaag agttagattt
     1141 gtttaccttt tcagatctcg ttggccctgg tttacccttg tatactccta acggggccgt
     1201 attaattgaa gaattgcgaa atgctttaaa tgaaataggt agacaatatg gtatgaggca
     1261 agtgtctata ccgcatattg ctaagataga actatatgaa acatctgggc atgctcagaa
     1321 atttagcggt gaattatttc atgtaaagag tcattatgaa caagattttg ttttaaagcc
     1381 tgtaaactgt ccgcaccata cccagatata cgctggacac cctagaagct acagggattt
     1441 gccactgcgc tacgttgagc agactatgca atatcgggac gaaaagccag gccagctcgg
     1501 cgggcttcaa agaacaagag gttttacggt agatgatgga cacacatttt gtcgtgctga
     1561 tcaaatcaag gaagaagctt ctatcatcgt tgaaatcatc cgtgattttt atgaatctct
     1621 tgggttgtgg ggtaatcatt gggtgtcact ttcggttagg gattatgcta acccagatgc
     1681 gtacattggg gatgcagcgg actgggaagt tgcagagcaa atgttacaag aggtatcaga
     1741 catccatgga ttaggtgcta aaaaaatgga aggagaagct gcaatatatg gtcctaaact
     1801 agactttatg tttaaagatg cgctaggccg tgaaacgcag ctcgcgacca ttcagctaga
     1861 ctttgctatg cccaaacgct ttgggctaac gtatgttaat gaagatggca aagacgctat
     1921 cccagtaatg atacatcgtg caattctcgg ttcgtttgag cggtttatta tgctccttat
     1981 agagcactat gctggcagat ttccagtttg gctggcccca gaacaggttc gttttataac
     2041 ggttaatcaa acaactccgg tagtacaagt ggcagagaaa gctcatgaaa aagcaaaaca
     2101 gcttggttta cgcaccagtg tagataatgc aaatgactcc gtaggaaaaa agatacgtaa
     2161 tgctgaaaag atgaagatac catacacaat tgttattggt gaaaaagaag tcgaaagtgg
     2221 tgaaacaaca ccgcgtattc gtaatgacat agaagtacag ctggagcatc cgataaaaat
     2281 tgataatttc ttaaaaacag tagccaacga agcaaaaaca cgaaccacta aaacgacatt
     2341 atagcgaacc atgcaaccga ggagtgattt tacaaaagct gtgtatgcag aaatagccaa
     2401 agtaccagca ggctctgtta tttcgtatag ccaacttgct gtttggtgtg gtcacccagg
     2461 ggctgctcgg gtggtgggcc aaattgcgca ttttggccca ccacccgagc agcccctg
//
LOCUS       DAOPLS010000108         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_48800_length_2478_cov_3.359884, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000108 DAOPLS010000000
VERSION     DAOPLS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48800_length_2478_cov_3.359884"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..859)
                     /locus_tag="PKV52_02855"
     CDS             complement(92..859)
                     /locus_tag="PKV52_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99184.1"
                     /translation="MTTFTSFSRDKARIEDRIGDNNKMLSGTLIMFLTGLALVILSVG
                     FAIPTDLFSSARWYDASGIRLVFEAENGFLANVSQHLPQKVSLESTVFALLLFFGQIM
                     LGIKAAMAGSWILDDSQYENVGLFRTLILIGRGDVVPEKLNAEKVTHFIMLVLLVALD
                     TFTDTDWKLSGSTANPFLALALSIFYNNLGSEWALVEGFRRAWAGGRLLWLGWRGEIT
                     FTNDIAPQTNQRNNKQRGNNQQQVNHRQHPSFIPPTE"
     gene            943..2010
                     /locus_tag="PKV52_02860"
     CDS             943..2010
                     /locus_tag="PKV52_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99185.1"
                     /translation="MSNLAGTTKKKSLRDILNIASFIAMMLGIEAEDVEDTEDVEDEL
                     PTEDMAKSAFQVFKDLSGQWRWFGWVSNKWVDREGEILVDAAHKDFVEWLDRHPERAP
                     ELWLYHARGTAHKNRADWWDYKNGFLMFSGILTDEEAKRYKDNKEPLGMSHGFWVYKR
                     IGRYITKYRTFEVTVLPLNRAANIHTGFSILEEMKMEKFNPHQREFLVGKLGEELVSK
                     LETNTEEQARLLEEAGVDQKALAAEYNALVQSEQDATVKPMIKAVADGVVKELKLEET
                     FALITERLNALTSLQKELEALKAEVKGLKELDDAKLASAFSPLAPLDWGLSVQKSDAA
                     AAPNVEEIVAEKQYSWLEELK"
     gene            2010..>2478
                     /locus_tag="PKV52_02865"
     CDS             2010..>2478
                     /locus_tag="PKV52_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99186.1"
                     /translation="MDNMLQKFEAFLKAEYKHTVGPTGAHVGPNVHGPDSLFGVAGLE
                     RDVISSRVVGNGLAAVLPVIGSNTTTPMFPYITGKTAAAGSQVGSSCQLAPEAGHLKT
                     CIQTAKFGLYQFTTRELEVNTIGRLINAGERVDLRYINDPLAAQMGRIWLNIPD"
BASE COUNT          795 a          493 c          527 g          663 t
ORIGIN      
        1 gaaattaata aggatacaac aaccgatcaa caagcactca ttcttaccaa tatcgaaaac
       61 ggcattattt cattgtatga gtgctgcgaa actactcagt aggtggtata aaacttgggt
      121 gttggcggtg attaacttgc tgttggttat taccacgctg cttattatta cgttggttgg
      181 tttgtggtgc tatgtcgttt gtaaacgtaa tttcaccacg ccaacctaac cataacaagc
      241 gcccaccagc ccacgcacgg cgaaagcctt caactaatgc ccattccgat cctagattat
      301 tgtaaaaaat acttagtgcg agtgctaaaa atggattagc tgtgctcccc gacaatttcc
      361 agtcggtatc tgtaaatgta tcaagggcca ccaacaatac taacataata aaatgtgtta
      421 ctttttcagc atttaacttt tcaggtacta catccccacg cccaatcaaa attagggtgc
      481 ggaaaagccc cacgttttca tactgactgt catcaagaat ccagctaccc gccattgccg
      541 ccttgatccc taacataatt tgcccaaaaa acagcaataa agcgaatact gtactttcaa
      601 gcgatacctt ttgtggcaaa tgctgtgaaa catttgctaa aaagccattt tccgcttcaa
      661 ataccaatcg gatgccagat gcatcgtacc atctagcact tgaaaataaa tcagtaggaa
      721 tagcaaaacc aaccgacagt attaccaatg ccaaccctgt tagaaacata ataagtgtgc
      781 ccgacaacat cttattgtta tcgccaattc tatcttcaat tcgggcttta tcccgtgaga
      841 aacttgtaaa cgttgtcata aagtatattt taacgataaa aacaccattt gtcaacgggg
      901 aaacttgaca tataaatacc gcaaataaat attaaagtta gcatgagtaa cttagcaggt
      961 acaactaaga agaaatcact tagagacatc ttaaatatcg catcttttat tgctatgatg
     1021 ttaggcattg aagctgaaga tgttgaggac accgaagatg tcgaagatga attgcctact
     1081 gaggatatgg ctaaaagtgc atttcaggta tttaaggatt tatctggaca atggcgctgg
     1141 tttggatggg tatctaataa atgggtagat cgtgagggag aaatcttagt cgatgccgca
     1201 cataaagatt ttgttgaatg gttagatcgg catcctgaac gcgcaccaga actatggctg
     1261 tatcatgcac gaggcacagc acacaaaaac cgtgcagatt ggtgggatta taaaaacggc
     1321 tttttaatgt ttagcggcat tttaacagac gaagaagcaa aacgttataa agacaataaa
     1381 gagccattag gcatgtcaca cggtttttgg gtatacaagc gcatagggcg atacattaca
     1441 aaataccgca cttttgaagt aacggtattg ccattaaatc gcgcagcaaa catacacaca
     1501 ggatttagta ttttggagga aatgaaaatg gaaaaattca atccacatca acgcgagttt
     1561 ttagttggta aattaggcga agaactagtt tcaaaactag aaaccaacac tgaggaacag
     1621 gcacgcttgc tcgaagaagc aggtgttgac caaaaggcgt tagcagccga atataacgca
     1681 cttgttcaaa gcgaacaaga tgcaactgtc aaacctatga ttaaggcggt cgctgacggt
     1741 gtagtaaaag aattgaaatt ggaagaaact ttcgcactta ttacagaacg cttaaatgca
     1801 cttacatctt tacaaaaaga gttggaagca ttgaaagcgg aagtcaaagg gctgaaagaa
     1861 cttgacgatg ctaaattggc aagtgctttt tcaccgcttg caccgttgga ttggggcttg
     1921 agcgtacaga aaagtgatgc cgcagcagca cccaatgtag aagaaatcgt tgctgaaaag
     1981 caatattcat ggttggagga attgaagtaa tggacaatat gctacaaaaa tttgaagcat
     2041 tcttgaaggc tgaatataaa cacactgttg gcccaacagg ggcacatgtt ggccctaacg
     2101 tgcatggccc cgattcgctg tttggggttg ccgggttgga acgtgatgtt atttctagcc
     2161 gtgttgtagg caatggttta gctgctgttt tacctgtaat tggttctaat accacaacac
     2221 ctatgtttcc atacattact ggtaaaactg ctgccgctgg ttcacaggtt ggttcaagtt
     2281 gtcagttagc accagaagca gggcatctaa agacttgcat ccaaactgct aaattcggtt
     2341 tgtatcagtt cactacacgt gaactagaag tcaatacaat tggccgtttg atcaatgctg
     2401 gcgaacgtgt tgatttacgc tatattaatg acccactagc agcacaaatg gggcgtattt
     2461 ggttgaatat ccccgatg
//
LOCUS       DAOPLS010000109         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_48933_length_2474_cov_2.556842, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000109 DAOPLS010000000
VERSION     DAOPLS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48933_length_2474_cov_2.556842"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PKV52_02870"
     CDS             complement(<1..1047)
                     /locus_tag="PKV52_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99187.1"
                     /translation="MLTIRKFQTKKAAKAVVNKAHLFIADGSGSMYQAVDQLKHDIGI
                     WIDRLQDGDVVTVGWFSSLGQYDYFIRNATINSTNRARLRQLILDNIKARNMTCFSEI
                     LNVTNETIIGELTATNLPLYMTFFTDGYPTVGNLRQEETRTLAALKAIAPALTDAMFI
                     GYRYHNVSLMAAMAKAAGGQLIHAKDIREVGEASERFLDNDAGRVTTPYPTEPVLAFQ
                     IEADHVEYVTWFDGALHSKPGDVYVVEKATNFETCGVIGSYATAALLAELGQADLALQ
                     VLGEIGDIRLIDMLNNAILPQEIVRTKNAIVEAIFDQTARFRDGYAAGYVPAPDAFCL
                     LDLIRVLDDVNAELV"
     gene            1384..1806
                     /locus_tag="PKV52_02875"
     CDS             1384..1806
                     /locus_tag="PKV52_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99188.1"
                     /translation="MTIEGIAAIAALLTSISAFVGYQVQRKSDQEKNALAREQAEVAK
                     EQAEQALFDKVWKMSQDNIKALQQDRDRLAEKVETLQLAIDIERDKRRAVELQLQNLM
                     LAVNNAQTGLILLAGQIVEMGGEPRWEPPKDLFNVGNW"
     gene            1790..>2474
                     /locus_tag="PKV52_02880"
     CDS             1790..>2474
                     /locus_tag="PKV52_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99189.1"
                     /translation="MSAIGKLFEEVVNENIIRVKWPNRDEYRNSRTGRVFKPQNDIVA
                     KHVFERDTPRHLYFMSGEGAGKTATLSVLVLEKLRRGMSGAFVCVDLPMLRKVWDEFK
                     LWIPWDCVIPQHRYMASAAWSPYQLFKIVFMSEMGMTAELTIGGLGDNISKWESLNIN
                     FLAGDEFRSVPKDDIMKVALGRMRVRGPNGEPPQLIIGSTPTYDDHWMYDYFGPITGN
                     NDPLLGFKKE"
BASE COUNT          748 a          501 c          516 g          709 t
ORIGIN      
        1 caccaattca gcattcacat catctaacac acggattaag tctaacaaac aaaaagcatc
       61 gggtgctggc acataaccgg cagcataccc atcccgaaat cgtgctgttt gatcaaaaat
      121 tgcttcaaca atggcgtttt ttgtacgcac aatttcttgc ggcaaaatag cgttatttaa
      181 catgtcaatt aggcggatgt cgccaatttc acccaagacc tgcaatgcta agtcggcttg
      241 gcctaattct gccaacagcg ccgcagtagc ataactaccg attacaccac atgtctcaaa
      301 attagtggct ttttcaacca catacacatc acccggcttt gagtgcaatg caccatcaaa
      361 ccatgtaaca tactcaacgt gatcggcttc aatctgaaaa gccaatacag gttcggttgg
      421 atatggtgtt gtaacacggc ctgcatcgtt atctaaaaag cgttcacttg cttcaccaac
      481 ttcacgaata tccttggcgt gtatcaactg tccaccggcg gcttttgcca ttgcagccat
      541 taaactaaca ttgtgatacc gataaccgat aaacattgca tctgtcaaag ctggggcaat
      601 cgcttttagt gctgccaaag tacgtgtttc ttcttgtcgt agattaccaa ccgttggata
      661 accgtcagta aagaatgtca tataaagtgg taaatttgtt gctgttagct ccccgataat
      721 tgtttcatta gttacattta gaatttcact aaagcatgtc atgttacgtg ctttaatgtt
      781 gtccaaaatt aactgccgca atcgtgcccg attggtacta ttgattgtgg cattacgaat
      841 aaaataatca tattgcccca aactgctaaa ccaaccaaca gttacaacat caccgtcttg
      901 taatcgatca atccatatac ctatgtcatg ttttaattga tcgactgcct gatacattga
      961 accgctacca tctgcaataa atagatgggc tttgtttaca actgcctttg ctgctttttt
     1021 ggtttggaat ttacgaatcg ttaacatgcg ttcattatac catattttaa tctagttagc
     1081 aatgccgtat tgtaaactaa tatgtaatga ttgtcagtac acgcataatc cttagttatt
     1141 gtatattcat agtagcacta ctgggattgt tgacactatt atatttagta gtgaaaaata
     1201 aaattgggcg tgggtatttg gtcggccccg ctattgtact aatacacacc gctgcttttt
     1261 atgggtatgt attgtttgat gacaggttga catcagaaat aaacgcgctt tggtcatcaa
     1321 ttttgcggct acaagaggtt tttactgtac ttacaatcct actagccata ggagcaaaca
     1381 aaaatgacca ttgaaggaat tgcggcgatt gcggcgttat tgacatcaat tagtgcgttc
     1441 gttggctatc aggtacaacg taaaagcgat caagaaaaaa acgcactagc aagagaacag
     1501 gcagaagtag caaaagaaca ggcagaacaa gcattatttg ataaggtttg gaaaatgtca
     1561 caagataata ttaaagcatt acaacaagat cgtgaccgcc ttgcagaaaa agttgaaacc
     1621 ttacaactgg caattgatat tgagcgcgat aaacgccgtg ctgttgagtt acaattgcag
     1681 aatcttatgt tggctgttaa taacgcccaa acaggcttga tattgttagc agggcaaatt
     1741 gtagaaatgg gcggtgaacc gcgttgggaa ccaccgaagg atttgtttaa tgtcggcaat
     1801 tggtaaatta tttgaagaag tagttaatga aaacattatt cgagtaaaat ggcctaaccg
     1861 agacgaatat cgtaacagtc gtacagggcg tgttttcaaa ccacaaaacg acatagtagc
     1921 aaaacatgtt ttcgagcgag acaccccgcg ccacttatat ttcatgtcag gtgaaggggc
     1981 aggtaaaaca gctacactgt ctgttttggt attagaaaaa ctacgcagag gtatgtcagg
     2041 cgcatttgtt tgtgttgatt tacccatgtt acgtaaagtg tgggatgaat tcaaactatg
     2101 gattccttgg gattgtgtga ttccacaaca tcgttatatg gcgagtgcgg cttggtcgcc
     2161 atatcaacta tttaagatcg ttttcatgtc ggaaatgggt atgactgccg aactgacaat
     2221 tggtgggttg ggcgacaata tcagcaagtg ggaatcgtta aatatcaact ttttagccgg
     2281 tgatgaattc cgcagtgtgc cgaaagatga cattatgaaa gttgccctgg ggcgtatgcg
     2341 tgtacgcggc cctaacgggg aaccaccaca actaatcatt ggtagcaccc caacatatga
     2401 tgaccactgg atgtatgact attttggccc aattacaggt aacaatgacc cacttttggg
     2461 tttcaaaaaa gaat
//
LOCUS       DAOPLS010000110         2425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_50341_length_2425_cov_3.340928, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000110 DAOPLS010000000
VERSION     DAOPLS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50341_length_2425_cov_3.340928"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKV52_02885"
     CDS             <1..445
                     /locus_tag="PKV52_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HPD99190.1"
                     /translation="DAIFSPVLRVRYKVEDTRVGQITDLDKLIITIDTDGTITPKDAF
                     EEAAAILVNQYTALAGSTRVEAVPASKTVAPSFNADIEDEPSELLVSIEDLNLTARTT
                     NALINNDIHTLKDLFSLSDAELRDLKGFGQKALDEVKDKLAELEL"
     gene            449..985
                     /gene="rplQ"
                     /locus_tag="PKV52_02890"
     CDS             449..985
                     /gene="rplQ"
                     /locus_tag="PKV52_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPD99191.1"
                     /translation="MHRHGYKGRKFSRERDQRRALIKGLASNLIDHGAITTTTPKAKE
                     LVPYFEKLITKAKKGDLHNRRQVISKVATIESAHKLVDEIAPKLTARSSGHLRINKKD
                     NRRGDNAQMATVSFVDDISTAKDDAKPTTKKATSLTKASVAPKKASTVVPSAPKKRVA
                     THTNKTATRAKGVTKGDR"
     gene            985..1416
                     /gene="rplM"
                     /locus_tag="PKV52_02895"
     CDS             985..1416
                     /gene="rplM"
                     /locus_tag="PKV52_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPD99192.1"
                     /translation="MKTYSAKPSEVTRTWFLVDASEAPLGRVATEVAQHLIGKKKPMF
                     TQHIDCGDHVIVINADKLVVTGNKLQDKKYYRHSQYPGSLKTTTLQEQIDKDSTAVIV
                     AAVRGMLPKNKLITERLKRLKVYAGNEHNHAAQQPKKVSVL"
     gene            1416..1814
                     /gene="rpsI"
                     /locus_tag="PKV52_02900"
     CDS             1416..1814
                     /gene="rpsI"
                     /locus_tag="PKV52_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004795241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPD99193.1"
                     /translation="MAKQDYFYALGRRKSATARVRLSGGSGHITINSKPAAEYFDGSE
                     YLLDELNQPFSLLDKEKQFDVSVVVAGGGLAGQADAIRLGIAKSLVLVNEDFKSTLKR
                     ADQLSRDSREKERKKFGLKGARKQRQFTKR"
BASE COUNT          827 a          512 c          514 g          572 t
ORIGIN      
        1 agacgcaatt tttagcccag tacttcgtgt acgatacaaa gtagaggaca cgcgtgttgg
       61 ccaaataacc gatcttgata aactcatcat aaccatagac actgacggca ccattactcc
      121 taaagatgcc tttgaagaag cagctgcaat tcttgttaat caatatactg cacttgctgg
      181 ttctacacga gtagaagctg tgccagccag taagacggtt gcaccaagct ttaatgcaga
      241 catcgaagat gaaccatcag aactgctagt gtctatagaa gatttaaatc taactgcccg
      301 caccacaaat gcactcatca ataacgacat tcatacactt aaagatttat tttctttaag
      361 cgacgcagaa cttcgtgacc taaagggctt tgggcaaaaa gcacttgatg aagtcaagga
      421 caagttggca gaactggagt tataggcaat gcacagacac ggatacaaag gtcgtaaatt
      481 tagccgcgaa cgcgatcaaa ggcgagcact catcaagggc ttagcttcta atttgataga
      541 tcacggtgcc attacaacta ccacgccaaa agcaaaagaa ctggtaccat actttgaaaa
      601 actcatcaca aaggcgaaaa agggcgattt acacaatcgc cgccaagtga tttcaaaagt
      661 agcaaccata gaaagtgccc ataaactggt agatgaaatt gcacccaaac ttactgcacg
      721 cagctctgga catttacgga tcaacaagaa agataatcgt cgtggcgata atgcacaaat
      781 ggcaacagta tcatttgtag atgatatttc tacagccaag gatgatgcta aacccacaac
      841 caagaaagca accagcctaa ccaaagccag tgtagcacca aagaaagcgt ctactgttgt
      901 accatcagca cctaaaaaac gcgtagcaac ccacacgaac aaaaccgcaa cgcgggctaa
      961 aggtgttact aagggggaca gataatgaaa acctatagcg ctaagccatc agaagtgaca
     1021 cgaacatggt tcttggttga tgcatcagaa gctccattgg ggcgggtagc gacagaagta
     1081 gctcaacacc taattggtaa aaagaaacca atgtttaccc aacacatcga ttgtggcgac
     1141 cacgttatcg ttattaatgc agataaactc gtcgtaacag gcaataaatt gcaagataaa
     1201 aaatactaca ggcatagtca atacccaggt agcttaaaga caactacctt acaagagcaa
     1261 atcgataaag attctacggc agttattgtc gctgcagtac gtggtatgtt accgaaaaat
     1321 aagctcatca cagagcgtct taaacggctt aaagtatatg ctggtaatga gcacaatcat
     1381 gccgcccagc aaccaaagaa agtgagtgta ttgtaatggc aaaacaagat tacttttatg
     1441 ctcttggcag acggaaaagt gctacggcac gtgttcgctt aagtggtggc agtggacaca
     1501 taactattaa tagcaaacca gctgcagagt attttgatgg aagtgaatat ttattagacg
     1561 aactaaacca accattttct ttattagata aagaaaagca attcgacgta agtgttgtgg
     1621 tagcaggtgg tggtttagct ggccaagcag atgcaatccg ccttggtatt gcaaaaagct
     1681 tagtccttgt aaatgaagat tttaagagta ccctcaagcg agccgatcaa ctatcacgcg
     1741 attcgcgtga aaaagagcgc aagaagttcg gtcttaaagg tgcacgtaaa cagcgtcaat
     1801 ttaccaaacg gtaaaacgaa tatctctaca ctgtaaacaa cttcaatgtc taaccaaaga
     1861 caattattag tagtataaaa attgacgaaa tactaaataa ttggtataat aacgttactg
     1921 gcattgccag gtctgttctt tggtagtaaa aatttattgc aatggctatc ttttatgata
     1981 gtacttgtgc gataggttgg gtacgttgta gccaatcaat cgcacaagta ctctagagta
     2041 gaaaggtagt aaaatgggaa gcccttatgc tctcatgttt ggactgatta ccttaattgt
     2101 agtattcttt tatgtccttg gatatgtact tatccactag acttcatgta gtaaagcact
     2161 gaacgggcag taaacccgga caagcataca aagtaaacat gcccggcccg cgcgaggcct
     2221 agagatgcta ccaggggcaa taacaagcct tgggggtcat catcgcgcgc gaagacgcca
     2281 agcaaagaac agaaccggcc cgacactcca cgcgtagagt cgggcctctt tttaaagcga
     2341 ctagtatagt agtaaccgta gtagacacat aaaaaacatt tgtacgtagc tagtatgagg
     2401 cataatacta tcgcgtatca ccagc
//
LOCUS       DAOPLS010000111         2411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_50746_length_2411_cov_4.217317, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000111 DAOPLS010000000
VERSION     DAOPLS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50746_length_2411_cov_4.217317"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKV52_02905"
     CDS             complement(<1..398)
                     /locus_tag="PKV52_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPD99194.1"
                     /translation="MLTIIIVAILAIAISVILHEVTHGYVAKYLGDSTAEDEGRLTLN
                     PAVHIHPFFTLALPLLLAILQQPIFGAAKPVPVQAHRLKGEEFGMALVAVSGPLVNLC
                     LALIGGVIFSFSNISGGLWELWWSYFIQIN"
     gene            491..679
                     /gene="rpmB"
                     /locus_tag="PKV52_02910"
     CDS             491..679
                     /gene="rpmB"
                     /locus_tag="PKV52_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPD99195.1"
                     /translation="MQVCDITGKGKQYGNNVSFSLRHTKKVWKPNLQSKTVVVDGKKV
                     KLKISTQAMRTLKKKGLL"
     gene            complement(715..1329)
                     /locus_tag="PKV52_02915"
     CDS             complement(715..1329)
                     /locus_tag="PKV52_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD99196.1"
                     /translation="MEIRYFGANCVKLTTKKASIIVDDTKDKGAYITTAKDIAIQTNK
                     ALSYGEGAVFEIQSPGEYEVSEVAVTGIASTLHHDPDKQSVIYSLHIDGFSVAIIGHT
                     EAQLDEAKLEKLGVVDVLIIPVGGHGYTLDAVEATKLIKEIEPKIVIPTHYEDSSVTY
                     EMPQAPLEEFLKTYGASDTETLDVLKLKDAMFPEKTQLVILNKQ"
     gene            complement(1347..1997)
                     /locus_tag="PKV52_02920"
     CDS             complement(1347..1997)
                     /locus_tag="PKV52_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPD99197.1"
                     /translation="MIEQLFGSKTRVKLLGLFYQNPNRSFYVREITRKIDEQINSVRR
                     ELANLLSIGIISSDTANNKLYYEVNQKFEHYDSLSEMFGGKPKTKRSKKKQPETDESK
                     SEVASEPIVEQIDTFADATIQNIVRAGNVRLLVFTGQFTRDETADADIIVIGDMNIPN
                     LEHAITELEKSEKKELRYCVFTPEEYMYRKQVNDRFLANILVAKKIVHLDRDNYLQ"
     gene            complement(2041..>2411)
                     /locus_tag="PKV52_02925"
     CDS             complement(2041..>2411)
                     /locus_tag="PKV52_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HPD99198.1"
                     /translation="GILDAYTDAGDKRIIGNISTKSHWGTLVGYENHSGKTYLAEGIK
                     QLGVTKIGQGNNGEDTTEGAQQNNVYGSYLHGPLLAKAPQFADALLEKAVQNIGVHKL
                     SLLDDSIADAAKHVALRRPR"
BASE COUNT          697 a          495 c          445 g          774 t
ORIGIN      
        1 atattaattt gtataaaata gctccaccat agctcccaaa gacctccact aatattagaa
       61 aacgaaaata ttacaccgcc tattagagcc aagcataagt ttacaagtgg accagacaca
      121 gcaacgagcg ccataccaaa ttcttctcct tttaggcgat gcgcttgcac aggaactggt
      181 ttggcggcgc caaaaatcgg ttgctgcaaa atcgccaaca gtaaaggcag tgcgagggta
      241 aaaaatggat gaatatgaac cgctgggttg agcgtaagtc gcccctcatc ttctgcagta
      301 ctatcgccta aatattttgc gacatagcca tgagtgactt cgtgtagtat aactgatatt
      361 gctattgcaa gtatggcgac aatgataatt gtaagcatat gtattagtat aacaaatctc
      421 accccttgac ttcttgagat aaaatacgta caataaacag ttgaagtttt gttaattaaa
      481 ggtaataact atgcaagtat gtgacatcac cggcaaaggt aagcaatacg gaaataatgt
      541 aagtttttcg ctgagacata ctaaaaaagt atggaaacca aacttgcaat ctaaaacggt
      601 agttgtagat ggcaagaagg ttaagctaaa aattagtacg caagccatgc gtacactcaa
      661 gaaaaaagga ctgctgtaaa cagcagtcct ttttcttcgt cttacgaaat taccttattg
      721 cttgttgaga ataacaagtt gtgtcttttc tggaaacatg gcgtctttga gctttaagac
      781 gtctaacgtc tctgtatcag atgctccgta cgtcttcaag aattcttcaa gaggagcctg
      841 tggcatttca tacgtaacac tactgtcttc gtaatgtgtg ggaataacaa tttttggttc
      901 tatttcttta atcagctttg ttgcctcgac agcatctagt gtataaccgt gcccaccaac
      961 agggataatg agaacgtcta caactccaag tttttctaac ttagcctcat ctagctgagc
     1021 ctcagtatgt cctataattg ccacagaaaa tccatctatg tgtaacgaat aaataacgct
     1081 ctgtttatca ggatcatggt gtaaggtaga ggcaatacct gtcaccgcca cttcacttac
     1141 ttcatattcg ccaggtgatt gaatctcaaa aacagctcct tcaccatagg atagtgcttt
     1201 atttgtttga atggcaatgt ctttggcagt cgttatatat gcacctttgt ccttggtgtc
     1261 atctactata attgaggctt ttttcgttgt tagctttacg cagttcgcac caaaataacg
     1321 tatttccata tcacatgctc ctttttttat tgtaaataat tgtctctatc taaatgtacg
     1381 attttcttcg ctactaagat atttgctaaa aatctatcat ttacttgttt ccgatacata
     1441 tattcttctg gtgtaaacac gcagtatcta agttctttct tttctgactt ttctagttcg
     1501 gtaatggcat gctctaagtt aggaatattc atatcaccaa tgactataat atctgcatcg
     1561 gcggtttcat cacgcgtgaa ctgacccgta aaaacgagta agcgcacatt acctgcacga
     1621 acaatatttt gaatggtagc atctgcaaac gtatcaattt gttctacaat cggttcactg
     1681 gcaacttcgc tttttgattc gtcggtctct ggttgcttct ttttactacg cttggttttt
     1741 ggcttgcctc caaacatttc tgataaagag tcataatgtt caaatttctg gtttacttca
     1801 taatatagtt tattgttcgc cgtatctgaa ctaattatgc caatactaag tagattagct
     1861 aattctctac gtacagagtt gatttgttcg tcaatcttac gtgttatttc cctcacataa
     1921 aaagaacggt ttgggttttg gtaaaataat cccagtaact tcacacgtgt ctttgagcca
     1981 aacaattgtt ctatcataat catattgtat caatagacgg tacaaaacaa cagacccgaa
     2041 ctatctaggg cgacgtaaag cgacatgctt ggcggcgtct gctatagaat catctagtaa
     2101 cgagagcttg tgaacaccaa tgttctggac agctttttct aataaagcat cagcaaattg
     2161 tggtgctttt gccaagagcg ggccatgtaa ataagaaccg tatacattgt tttgttgcgc
     2221 accttcagtc gtgtcttctc cgttattgcc ctgtcctatt tttgttacac caagttgttt
     2281 gatgccttca gctaaatacg tcttgccgct atgattttcg tagccaacta atgtgcccca
     2341 atgagatttt gtgctaatgt tgccaatgat tcgcttatca cctgcatcag tgtaggcatc
     2401 aagtatgcca a
//
LOCUS       DAOPLS010000112         2372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_51964_length_2372_cov_4.233060, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000112 DAOPLS010000000
VERSION     DAOPLS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51964_length_2372_cov_4.233060"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="PKV52_02930"
     CDS             <1..1979
                     /locus_tag="PKV52_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPD99199.1"
                     /translation="TKSRRKGGKSVLDVFGSDLTALAAEDKLDPVVGREDAIKRTITI
                     LNRRSKNNPVLIGEPGVGKTAVVEGLAQRIVAEEVPDSLLEKRIVVLDLAAMIAGTKY
                     RGEFEERLKKVMDELMADRKIIIFIDEMHLLVGAGAAEGAIDAGNILKPALARAKIQV
                     IGATTTSEYHKHIEKDAALERRFQPVVIPPTTTTETLAILKGLKKHYEDYHGVRISDA
                     TIEDAVYFADRYISDRYMPDKAIDLLDEAAALLRVDKGKTPKEYRQLIKEIRLFTVRR
                     DDAVEQEDYEKAAKYKQRISAAQARLDELKSKLGKHKRLELSGDDIARVVHQATSIPL
                     TKLMRQEAKNLLKLESHLGRYVIGQEEAINAIARAIRRNRSGVGSEKRPIGTFMCMGP
                     TGVGKTELARVLAREFFGKEDALIKIDMSEFGERHTVARLVGAPAGYVGYEEGGQLTD
                     KIRRQPYSLVLFDEIEKAHPEVFNMLLQIFEDGELTDAKGRKVSFRNTIIMLTSNIGA
                     KLLQKEVNLGFASKANDKSVDELHAENSEKVKEELKKHMRPELINRIDKIIVFKALNK
                     QAVRKIIDVQINELAQRLQRQKIGIHLSAAAKNWLLKKGYDVHNGVRPMRRAIQDEIE
                     DPVADNILDGTFTAGDVITVTTDKKLLTFSVIHE"
     gene            2001..>2372
                     /locus_tag="PKV52_02935"
     CDS             2001..>2372
                     /locus_tag="PKV52_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99200.1"
                     /translation="MTRTVKWLWTTVFIAMVVSIVFVWFNRFTIFDAYVLRSYTPSPE
                     IVQLAKDSGMNETGAEIFYSHVPKITEQAEFNSVCTSSEATIVLGCYTGFDFNKDTDI
                     YIYNVQKPELAGIKEVTASHEM"
BASE COUNT          785 a          436 c          556 g          595 t
ORIGIN      
        1 caactaagtc acgtcgcaaa ggtggtaaaa gtgttctcga tgtatttggg agtgacctga
       61 cggctctcgc agctgaagat aagctagatc cggttgttgg tagagaagac gctataaaac
      121 gtaccatcac gatattaaac cgcaggagta aaaataaccc tgttttgatt ggagaacctg
      181 gggttggtaa aacagccgtc gtagaaggcc ttgctcaaag aattgttgca gaagaggtac
      241 ctgacagctt actagaaaaa cgcattgttg tattagactt agctgccatg attgccggta
      301 ccaaataccg tggtgagttt gaagagcgcc taaaaaaggt tatggacgag ctaatggcag
      361 atagaaaaat tattatattt attgatgaga tgcaccttct cgttggcgct ggggcggcag
      421 aaggagctat tgatgcaggg aatatactta aacctgcgct tgcgcgtgcc aaaatacaag
      481 tcatcggcgc cacgacgacg agcgagtatc ataaacatat agaaaaagat gctgcgctcg
      541 aacgacgttt tcaacctgtc gttataccac cgaccactac cacagaaacg ctcgcaatct
      601 taaaagggct taaaaaacac tacgaagatt accatggtgt tcgtatttct gatgcaacga
      661 tagaagacgc ggtatatttt gccgatagat acataagtga tcgctatatg ccggataagg
      721 caattgattt gcttgatgaa gcagccgcat tattgcgcgt agataaaggc aaaacaccta
      781 aagaatatcg tcaacttata aaagaaatac gcttatttac tgttcgtcgt gatgatgcag
      841 tagagcaaga agattacgaa aaagccgcaa aatataaaca acgtattagt gcggcacagg
      901 cacgtcttga tgaactaaaa agcaagctgg gcaagcataa gcgactagaa cttagcggtg
      961 atgacattgc gagggtagta catcaagcaa cgtcgattcc gcttacaaaa ctcatgcgtc
     1021 aagaggcgaa aaatcttcta aaattagaat cacacctcgg ccgctatgta attgggcaag
     1081 aagaggcaat taatgctatt gcacgggcta tacgaagaaa tcgttctggt gtagggagtg
     1141 aaaaaagacc aatcggtaca tttatgtgca tgggtccaac aggtgtaggg aaaactgagc
     1201 ttgcgcgtgt cctggcacga gagttttttg ggaaagaaga tgcactgatt aaaattgata
     1261 tgagtgaatt tggtgagcgc cacacagtgg cacgtttagt tggcgcccca gctggttatg
     1321 tagggtacga agaaggtggt cagttaactg ataaaattcg tcgccaaccg tatagccttg
     1381 tgttatttga tgagatagaa aaagcgcacc ctgaagtatt taatatgctt ctgcagattt
     1441 ttgaagacgg cgaactcaca gatgcaaaag gtcgtaaggt gagctttagg aatacgatta
     1501 tcatgcttac gagtaatatt ggtgctaagt tgttgcaaaa agaggttaac cttggctttg
     1561 ccagcaaggc aaatgataaa tctgtcgatg aactccatgc cgaaaatagc gaaaaagtaa
     1621 aagaagaact gaaaaagcat atgcgtccag agcttattaa tcgtatagat aaaataatcg
     1681 tctttaaagc gctcaacaaa caagctgtgc gtaaaattat tgatgttcaa attaatgaat
     1741 tagcccaacg tttgcagcgc caaaaaattg gcatacactt aagcgcagct gccaaaaact
     1801 ggctactaaa aaaaggctac gatgtacaca acggagtacg ccctatgcgc agagccattc
     1861 aagatgaaat cgaagatcca gtggcggata atatattaga cggaactttt acggcaggtg
     1921 acgtaataac tgttactaca gataaaaaac tacttacgtt tagtgtaatc cacgaataat
     1981 tcggtatact tatatatacg atgacacgga cagttaaatg gctatggaca acagttttta
     2041 tcgctatggt agtgtctatt gtgtttgtgt ggtttaaccg gtttacaata tttgatgcgt
     2101 atgtactccg ttcttacaca cctagcccag aaatcgtaca attagcaaaa gatagcggca
     2161 tgaacgaaac gggcgcagag atattttact cacatgtgcc taaaataact gagcaggctg
     2221 agtttaatag cgtttgtaca tcaagcgaag caacaattgt actagggtgc tatacaggat
     2281 ttgattttaa taaagacacc gatatataca tttataatgt gcaaaaaccg gagctcgcag
     2341 gcattaaaga ggtaactgct tcgcacgaaa tg
//
LOCUS       DAOPLS010000113         2370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_52029_length_2370_cov_3.426350, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000113 DAOPLS010000000
VERSION     DAOPLS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52029_length_2370_cov_3.426350"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>2370
                     /locus_tag="PKV52_02940"
     CDS             46..>2370
                     /locus_tag="PKV52_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD99201.1"
                     /translation="MILVINKNHPRVIKAKYKRLFDSSTLRTTLGLIAAVALIASIAL
                     VVLGFSVGWLVSIVPVVLLMLHEWYRHDLLVLPPTTQEAPEDIIDSSLLAVLPSQVDT
                     IDTLVSALKKTQEYWFLTNRYLLYPDIIQTIKPAENWLQTAIDVYRNSGSTHGVNAAH
                     MLAAIILCSPDKSLILSHTQNTEEDIIDGLHWQAYVQSLIASIKQRKNQGGIARDWAA
                     GYTPLLDSYAVNISRSIQYGGISHREIIGHTNVVSQMKAIFTSNGRANIALVGAVGVG
                     KSTIIQAFAESLLFDNKKAKIAYNQVYQIDIASLLTRVSPDRLEYTIRRLCAEAYHAK
                     NIVLYFDNAGAFFGAGGSVADATNIILPIIEAGRVRMIFSFSQLDWQYIQKAKSQIAA
                     LLNYQVVTPTNEQDTLRILENQALFTESQYNCTFTYRALKEVYLLANRYGPEIAMPGR
                     AISVMEDVARSCQDSLIQKTDVQKTVEQTTGIKIATADADDKNALLNLEEDLKKRVVG
                     QDVAIKELVSALKRSRTGIANPNRPIGTFLSLGPTGVGKTELSKSLADVYFGGSSGFV
                     RVDMNEYITQDSIHKLLSSESGFGSAFLETVRRQPFSVVLFDEIEKAHPDIVNALLQL
                     LDEGEIKDADNRTVSFKDAIVIATSNAGAEQIRDQLKNQLPIAQLQDTLVDTLIDKGT
                     FAPEFINRFDGVIIFSPLSQDQLKHIITIQLRNINNTLAAQGITVALDDSAVAWLIAQ
                     GYDERLGARPLRRMMQKTVETAVSNILLERQVQHG"
BASE COUNT          749 a          476 c          506 g          639 t
ORIGIN      
        1 tatccccgta ttaccaattg tttttatggt cttggcggga ataacatgat tctagtcatt
       61 aacaaaaatc acccgcgtgt cattaaggct aaatataaaa gactatttga cagctctacg
      121 ctaagaacaa cattaggact gattgctgct gttgcattga ttgccagtat tgcactagta
      181 gtgctgggtt tttcagtagg atggcttgtg tcaattgtgc cagttgtttt attaatgttg
      241 cacgaatggt atcggcatga tttacttgtg ctgccaccga caacgcaaga agctccagaa
      301 gacatcatag atagtagcct actagctgtg ctgccgagtc aagtagatac aatagatacg
      361 cttgttagtg cactgaaaaa aactcaagaa tactggtttt tgaccaaccg ttacctgtta
      421 tacccagata ttatacaaac aataaaacca gcagaaaact ggctgcagac tgcaattgat
      481 gtatacagaa atagtggttc tacacacggc gtaaatgctg cacatatgct cgcagcaatt
      541 atcctttgta gtccagataa atcactcatt ttatctcata cacaaaatac agaagaagac
      601 attattgatg gcttgcattg gcaagcgtac gtgcaatcgc ttatcgcatc aattaaacaa
      661 agaaaaaacc aaggcggtat cgccagagat tgggctgctg gctatacacc actgctagat
      721 agctacgcgg taaatatttc gcgctctatt cagtacggag gcatatcaca cagagagatc
      781 attggtcata caaatgttgt ttctcagatg aaagcgatat ttacaagtaa tgggcgcgct
      841 aatatcgcgt tagtgggtgc tgtgggggta ggcaagagca cgattattca ggcttttgca
      901 gaatcattat tgtttgataa taaaaaagca aagatagcgt ataaccaggt ttatcaaatt
      961 gacatcgcgt cactacttac gcgtgttagc ccagatcggc tcgagtatac tattcgcaga
     1021 ctttgcgcag aagcatatca cgctaaaaac attgtacttt attttgataa cgccggtgcg
     1081 ttctttggtg caggtggttc cgttgctgat gctactaata ttattctccc aatcatagaa
     1141 gcaggtagag taagaatgat attttcgttt tcacagttag actggcaata tatacaaaaa
     1201 gccaagtcac aaattgcagc attgcttaat taccaagtag tgacacccac caacgaacaa
     1261 gacacactcc gcatactaga aaaccaagcg ttatttactg aatctcaata taattgtacc
     1321 tttacgtaca gggcgttaaa agaagtctat ctactggcaa atcgatatgg cccagaaatt
     1381 gctatgcctg gccgtgcaat tagtgttatg gaagatgtag ctcgttcgtg ccaagatagt
     1441 cttattcaaa aaacagatgt tcaaaaaaca gtagaacaaa ccaccggtat aaaaattgcc
     1501 actgcagatg ctgatgataa aaacgcacta ctgaacctag aagaagactt aaaaaaacgc
     1561 gttgtcgggc aagatgtagc tattaaagag cttgtgtcag cgctcaagag gagccgtacg
     1621 ggcattgcaa acccaaatag gcctattggt acgtttttat cccttggacc aacaggagta
     1681 ggtaaaacag agcttagcaa atctcttgca gatgtatatt ttggtggtag cagtgggttt
     1741 gtaagggtag atatgaacga atacattacc caagatagta ttcataaact tctttcgtct
     1801 gagagtggtt ttgggtcggc ctttttagaa acagttcggc gccaaccctt tagtgtcgta
     1861 ttattcgatg aaattgaaaa agcgcaccca gatattgtga atgcactact gcagctatta
     1921 gacgaaggag aaataaaaga tgcagacaac agaacagtct cgtttaaaga tgcgatagtc
     1981 attgccacgt ctaatgccgg agccgaacag ataagagatc agctgaaaaa ccaactgcct
     2041 attgcacagc tacaagacac acttgtagat acgctcatag acaagggtac atttgccccc
     2101 gaatttatta accgttttga tggggtaatt atattttcgc ccctctcaca ggatcagctt
     2161 aagcacatta ttaccattca attgcgcaat atcaataata cacttgcagc ccaaggtatt
     2221 acggtagcat tagacgattc ggccgttgca tggttaatcg cacaaggtta cgatgagcgt
     2281 ttaggggcaa gaccactgcg acgtatgatg caaaaaacgg tcgagacagc tgtatctaac
     2341 atcttactag agcgtcaggt tcagcacgga
//
LOCUS       DAOPLS010000114         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_52106_length_2367_cov_3.772059, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000114 DAOPLS010000000
VERSION     DAOPLS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52106_length_2367_cov_3.772059"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKV52_02945"
     CDS             <1..530
                     /locus_tag="PKV52_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPD99202.1"
                     /translation="QRNKIAPIAITNGMAIGKRLTLQIVDSDTIRTRLKAGTLYVYAP
                     LQDVQNQTDAYVMATKKAMKRPLNKQAKEIIPDRVDFLARQFGYSYQSVHCKPLRSRW
                     GSCSNKKELTFNCYILMLPWEIIDYIIMHELSHTKQMNHSTQFWDEVASTTPDYILLR
                     KQLKALQPGVHAFYV"
     gene            556..1671
                     /locus_tag="PKV52_02950"
     CDS             556..1671
                     /locus_tag="PKV52_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD99203.1"
                     /translation="MSTTPKKNQSTNTSTAALEHERLTALINSMADAVIAVDELQKVV
                     IYNGAALNILDVNKGLNGKRIRALLHLLDKNNQIIDVEQLIDRTRTQFTTRDVRLKYP
                     NGETINLYLSIAPVHLGYAIRGQRGHVILMRDITREKSLEEERDEFISVVSHELRTPI
                     AIAEGNISNAQFSVDRASNVPDSVSTSLKAAYDQIIFLSGMINDLATLSRAERGKLTT
                     EIEAINAHDVLQDLKQAYQRQAEEKGLKLTIKADPHLELLRSSKLYVKEILQNFVTNA
                     IKYTQAGSIEISAHKKTGGVRFDVTDTGIGISKTDKQKVFDKFFRSEDYRTRQSSGTG
                     LGLYVTMKLARLIHAEVDLQSEVNEGSTFSVYFPNLD"
     gene            complement(1672..>2367)
                     /locus_tag="PKV52_02955"
     CDS             complement(1672..>2367)
                     /locus_tag="PKV52_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99204.1"
                     /translation="ARNVSMIGLVPKRVKSGLLAISIQPMRYWWEIALPNRCKKRPPV
                     LSTAWIAHRAALMQLGGFLSYKRSIIPEEHLARKFFKQSSYAFVRTSPTLQLTTQKNF
                     MSQWDTQVRTRYPHAHRRPETVLMQTLLLLLITWPFIALLLCVVAQADQLLLLLISAS
                     IVLLITSHVCINSITNPVANWFALINFPIAISLDILALHVSMFRYEFAKVIWKGRDVA
                     PKKLVVYTELPAL"
BASE COUNT          783 a          462 c          504 g          618 t
ORIGIN      
        1 agcaacgtaa caaaatagcc ccaattgcta ttacgaacgg aatggctatt ggcaagcgat
       61 tgacactgca aatagttgat tcagatacca ttcgcacgcg gcttaaggca ggtaccttat
      121 atgtttatgc accgctacaa gacgtgcaaa accaaacaga cgcttacgtt atggctacta
      181 aaaaagcaat gaaaaggcca ttgaataaac aagccaaaga gattattcca gacagagtag
      241 attttttagc tcgtcagttt ggctattcgt accagagcgt tcattgcaaa ccactcagat
      301 cacgctgggg atcgtgttct aacaaaaaag aacttacatt taattgctat atattaatgt
      361 tgccatggga aatcatagat tacattatta tgcatgaatt atcacacacc aagcaaatga
      421 accatagcac gcaattttgg gacgaagtag ccagtaccac gccagactat atattgctac
      481 gaaagcaatt aaaagcctta cagccaggtg ttcacgcctt ctatgtataa aagtagggta
      541 taattacaat taagcatgag cacaacacca aaaaagaacc aatctaccaa cacttctacc
      601 gctgcgctcg aacacgaacg gctaactgcg cttataaaca gcatggcaga tgcggtaatt
      661 gcagtagatg agctccagaa ggtggttata tataatggtg cggcactgaa cattcttgat
      721 gttaacaaag gtctgaatgg taaaagaatt cgtgcattat tgcatttatt agataaaaat
      781 aatcagatta ttgatgtaga gcagttgata gatcgaacca gaacgcagtt tacaacgcgt
      841 gatgtccgcc taaagtaccc taacggcgaa actattaacc tatatttaag tattgcaccg
      901 gtgcatttag ggtatgcaat tagaggccag cgtggccatg taatactaat gcgcgatatc
      961 accagagaaa aaagcctaga agaagaacgt gacgaattta tatctgttgt gagtcatgaa
     1021 cttcgtacgc caatagcgat agcagagggc aatataagca atgcgcagtt ttctgttgat
     1081 cgcgctagta atgttcctga ttctgtaagc acgtccttaa aagcagcgta tgaccaaata
     1141 attttcttga gtggtatgat aaatgatctt gcgacattgt ctcgcgccga gcgcggcaaa
     1201 ctcaccacag aaatagaagc tatcaatgca catgacgttt tacaggactt aaagcaagca
     1261 taccaacgcc aggcagaaga aaaaggttta aaactaacca taaaagccga cccgcattta
     1321 gaattgttac gttcaagcaa gctttatgtt aaagaaattt tgcaaaattt tgttaccaac
     1381 gcaataaaat acacgcaagc agggtctata gaaattagcg cacataaaaa aaccggtgga
     1441 gtacgttttg acgtaacaga tacgggcatt ggaattagta agacagataa acaaaaagta
     1501 tttgataagt tttttagaag cgaagattat cgtacacgcc aaagtagcgg aacagggcta
     1561 gggctttatg taaccatgaa gctagcacgg cttattcatg cagaagtaga tttacaaagc
     1621 gaagttaacg aaggctctac tttttctgta tattttccta atttagacta attaaagggc
     1681 aggtagttca gtatatacaa cgagcttttt gggagcgacg tctctcccct tccagataac
     1741 ttttgcaaat tcgtagcgga acatagaaac gtgcagcgcg agtatatcta ggctaatcgc
     1801 tataggaaag ttaataagtg caaaccagtt agcaactggg ttagtaatgc tatttataca
     1861 aacatgcgaa gtaataagta agacaatgct cgcactaatg aggagtaaga gtagttggtc
     1921 agcctgtgcc acaacacata acagcaacgc tataaatggc catgtaataa gtagcagtag
     1981 tagagtttgc atgagcactg tttctggccg acgatgagcg tgtgggtagc gagtgcgcac
     2041 ttgggtgtcc cattggctca taaagttttt ttgagtcgtt agctgcaatg tgggtgacgt
     2101 tcttacaaac gcatacgatg actgcttaaa aaattttcgc gctaggtgct cttctggaat
     2161 aatactacgt ttatatgaca aaaagccccc tagctgcata agcgctgcac gatgtgctat
     2221 ccacgcagta ctaagaactg ggggtctttt tttacatcta ttaggtaggg caatttccca
     2281 ccaatagcgc atgggttgta tagatatagc aagcagcccg ctttttactc ttttaggcac
     2341 taaaccaatc atagagacat ttcttgc
//
LOCUS       DAOPLS010000115         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_52910_length_2342_cov_3.663314, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000115 DAOPLS010000000
VERSION     DAOPLS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52910_length_2342_cov_3.663314"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKV52_02960"
     CDS             <1..199
                     /locus_tag="PKV52_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99205.1"
                     /translation="GKGRLSLVNPLSTSDFAQRLELLLYDDDVREMWRVWAAETIKQY
                     DFSVIAKQYESVYKQSLAQHV"
     gene            192..1304
                     /locus_tag="PKV52_02965"
     CDS             192..1304
                     /locus_tag="PKV52_02965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD99206.1"
                     /translation="MSKKLKIGFVLDDTLDTPDGVQQYVLTIGEWMRLQGHDVHYLVG
                     ESYRTDIQNVHSLAKNIRVTFNKNRLSIPLPTNKKVIAKTLNKLQLDVLHVQMPYSPL
                     FAGRVISAAPNTVKIVGTFHIVPATWLEKTGAKSLQLITKHTLKRFNSIISVSSAAQT
                     FAKTVFGLDTIVIPNTVNLQDFKHKPIYKSPLHIVFLGRLVERKGCEYFLKALSHLQT
                     TYSTVAPFNVTIAGKGPLLKRLQQYTLEHNLPMVTFAGFIDEKDKPALLASADIAVFP
                     STGGESFGIVLIEAMAAGSRVVLGGNNVGYKSVLGNNPSLLISPRDTAQFAQTLHKYI
                     HSKKDREMAYKWLQKQKDTYDVGVVGTSILHIYGIK"
     gene            1430..1579
                     /locus_tag="PKV52_02970"
     CDS             1430..1579
                     /locus_tag="PKV52_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99207.1"
                     /translation="MPTITSSIGTIQTYDAVGYDESFRDQLQLNEDTTLQSLTPLGIF
                     EYLQF"
     gene            complement(1568..>2342)
                     /locus_tag="PKV52_02975"
     CDS             complement(1568..>2342)
                     /locus_tag="PKV52_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPD99208.1"
                     /translation="IDIHTGGIDHIPVHHTNEIAQSECATGVRFANYWLHNNHLLADG
                     TKLSKSLGNSYTLNDVLAKGFSAADFRLFVLQSHYRSESNFTWQNLTAAQNRLRKWQA
                     YASRRFQVIQPLHTDTISKDLKSNKSAVMQKQEEVAKKEATTYLKAFDNAMDNDMDTP
                     NALAAVDNFLHIIDTHGMMSSIVPEFVKLLQNIADILGIDLFDEDISDVDKKAVQTRE
                     DYRAQKDWDKSDSIRHHLEEKGIYLTDTPHGVVWSRSKL"
BASE COUNT          684 a          460 c          464 g          734 t
ORIGIN      
        1 tggcaaaggg cggcttagcc tagtaaaccc tttgtctacg agcgattttg cacaacggtt
       61 agaactctta ttatatgacg acgatgtacg tgaaatgtgg cgtgtatggg ctgccgaaac
      121 gatcaaacaa tatgactttt cagtcattgc aaagcaatac gaatcagtct ataagcaatc
      181 acttgcccaa catgtctaaa aagttaaaaa ttggctttgt tttagacgat acactagata
      241 ctccagacgg cgttcagcaa tatgtgctaa cgataggtga atggatgcga ttacaaggac
      301 atgatgttca ttatcttgtc ggtgaatcat atcgcacaga catacaaaat gttcactctt
      361 tagcaaaaaa tattcgcgtg acttttaata agaatcggtt aagtattcca ttgccaacga
      421 ataaaaaagt aattgccaaa acgctcaata aactgcagct agacgtacta catgtgcaga
      481 tgccatatag tccgctattt gctggacgtg tcattagcgc cgcgccgaat acggtaaaga
      541 ttgttggtac gtttcatatt gttcccgcga catggttaga aaaaactggt gcaaaaagtt
      601 tgcaactcat taccaaacat acactaaaac gttttaactc aataattagt gttagctcag
      661 cagcgcaaac gtttgctaaa acagtatttg gcctcgatac tatagttatt cccaacactg
      721 taaatttaca agattttaaa cataaaccta tctataagtc accactgcat atcgtgttcc
      781 ttggtagatt ggtagaaaga aaaggttgcg aatacttttt aaaagctctc agtcatttgc
      841 aaactacata cagtactgtc gcaccgttca atgttactat tgctggcaaa ggccccttac
      901 tcaagcgctt gcaacagtac accttagaac acaacttacc tatggttaca tttgcaggat
      961 ttattgacga aaaagataaa cctgcacttt tagcgagcgc tgatattgct gtatttccta
     1021 gtacgggtgg tgagagtttt ggcatcgtgc ttattgaagc gatggccgca ggatctcggg
     1081 ttgtgcttgg cggcaataat gttggttata aaagcgtatt aggtaacaac ccttctcttt
     1141 taatatctcc gcgtgatacc gctcaatttg ctcaaaccct acacaagtac attcatagta
     1201 aaaaagaccg agaaatggcc tacaagtggc ttcaaaagca aaaagatacc tatgatgttg
     1261 gtgtcgtcgg tacgtctatt ttacatatat atggtataaa gtaagcacga gcaatagtat
     1321 ttcgtttgcc tacagcatat aatcgtcaat atatatggta tctggagcga attttatagc
     1381 gattgtatgt atttggttag ttgcaatagt tgcagcggta tatattttta tgcctacaat
     1441 tacctcttct attggtacca ttcagacgta cgatgctgta gggtatgatg aatcatttcg
     1501 tgaccagctg cagctcaatg aggatacaac tctacagtct cttacgccgc ttggtatttt
     1561 tgaatattta caattttgat cgactccaca ccactccgtg tggtgtatcg gttagatata
     1621 ttcctttttc ttcaaggtga tgacgaatag aatctgattt gtcccaatct ttttgcgcac
     1681 gataatcctc tcttgtttgt acagcttttt tatctacgtc tgaaatatct tcatcgaata
     1741 aatctatacc caagatgtct gcaatatttt gtagcaactt cacaaactct ggcacaatag
     1801 acgacatcat tccatgggta tctataatgt gaaggaaatt atcaaccgca gcaagtgcgt
     1861 taggagtgtc catatcatta tccatggcat tatcaaacgc ttttagatat gtagttgctt
     1921 ccttttttgc gacttcttcc tgcttttgca tcacagcaga tttgttactt tttaagtctt
     1981 ttgaaatggt atcagtatgc aagggttgaa taacctgaaa acgacgtgaa gcataggctt
     2041 gccatttacg taaacgattc tgggcagcgg taagattttg ccaggtaaaa ttgctctcgc
     2101 ttctgtaatg gctctgtaat acaaatagtc taaagtcagc ggcgctaaat ccttttgcta
     2161 ggacatcatt gagtgtatat gaattgccta agcttttact taatttagtt ccatctgcga
     2221 ggaggtgatt gttatgaagc caataattcg caaaccgcac accagtagcg cattcacttt
     2281 gagcgatttc gttggtgtgg tgcactggta tatggtctat gccacctgtg tgtatatcta
     2341 tg
//
LOCUS       DAOPLS010000116         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_53406_length_2327_cov_4.089789, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000116 DAOPLS010000000
VERSION     DAOPLS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53406_length_2327_cov_4.089789"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..2203)
                     /gene="uvrB"
                     /locus_tag="PKV52_02980"
     CDS             complement(230..2203)
                     /gene="uvrB"
                     /locus_tag="PKV52_02980"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPD99209.1"
                     /translation="MASFDLQTSYEPTGDQPKAIAALTKGIQQGLQHQTLLGVTGSGK
                     TFTMANVVQNTQKPTLVLAHNKTLAAQLYNEFKVFFPNNAVHYFVSYFDYYQPEAYIA
                     RSDTYIEKDSQINEEIDRLRHAATSALLSRRDVLIVASVSCIYGIGSVDDYGTMAIRV
                     AVGERRLRDKFLRQLTDIQYHRNDIAFTRSTFRVRGDVVDVFPAGEESAYRIDFFGDE
                     IDRITKINPLTGEIEASLESYTIYPGSHYVTPQQKLKGAIANIQTELQQRLQYFNANN
                     QLLEAQRLAQRTKFDLEMLEETGFVKGIENYSRYLTNREPGEQPATLLDYYPDDFLLM
                     IDESHMTLPQVRGMYNGDRARKEVLVDYGFRLPSALDNRPLTFSEFEQHISQVVYVSA
                     TPAEYELSRSPEPVQQVIRPTGLLDPTIIVRPVTGQVDDLLAEIRERVAKHQRVLVTT
                     LTKRMAEDLSEYLEELGVKVQYLHSDVDTLDRIDILRDLRLGIYDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSEPALIQTIGRAARHIEGTVIMYADTITGSMKRAIDETN
                     RRRSIQEAYNTKHGITPRGIQKAIAETMRTELSSETKEAKTLKKDISKIPAEEYPLII
                     KDLTNQMELAAKNLEFEKAADLRDLITAIEKKM"
BASE COUNT          617 a          498 c          486 g          726 t
ORIGIN      
        1 catcttgcgt agatgataaa cctgcgatat tggcctttga ctcaataaaa aaaacgtatt
       61 ttgttatcta ccgttgatac tgcaagtgtg aaaggactta gcccaccaat ctgcttagct
      121 gttgacaata caaaactgcg ccattcttcg tgagtataag attctttggt tagcaagagt
      181 tcactaatgt tcatctaggt caatgatacc ttaaagtaac aaccttatgc tacattttct
      241 tttctatggc ggtaatgagg tcacgtaagt cggctgcttt ttcaaattct aggtttttag
      301 cggcgagctc catctgattt gtaagatctt ttataatcaa tgggtattct tctgctggga
      361 tttttgagat atctttttta agtgttttgg cttcttttgt ttcagatgaa agttcagtgc
      421 gcattgtttc ggcaatggcc ttttgaatac cacggggtgt tataccatgc ttcgtattgt
      481 atgcttcttg aatactacgg cgacgattgg tctcatctat ggcacgcttc atactacctg
      541 taatggtatc tgcatacata atgaccgtgc cctcgatatg ccgcgcagca cgcccaatgg
      601 tttgtattag ggcaggctca ctgcgcaaaa aaccttcttt atcggcgtct agtatggcaa
      661 caaggcttac ttcaggtaag tctaagcctt cgcggagcaa attaatacca acaagcacgt
      721 catatatgcc taaacgaaga tcacgcaaaa tgtctatacg gtctagcgta tctacatcgc
      781 tatgtaggta ctgcactttt acccctaact cttctaaata ttcacttagg tcttctgcca
      841 tacgttttgt aagcgtagtt actagcacac gctgatgttt cgcaacgcgt tctcttattt
      901 ctgctaataa atcgtctact tggcccgtta cggggcgtac aataattgtt gggtcaagca
      961 agcctgtcgg ccgaataacc tgctgtacgg gctccggtga acgtgacagt tcatattctg
     1021 ccggagtagc agatacatat acaacttgcg atatatgctg ctcaaactca ctgaatgtca
     1081 atgggcgatt atctagcgcg cttggtaaac gaaagccata gtctactaac acctctttgc
     1141 gggctcgatc gccgttgtac atacctcgca cctgtggtag ggtcatgtgg ctttcgtcaa
     1201 tcatcaataa aaaatcgtct ggatagtaat caagaagcgt cgctggttgt tcaccaggtt
     1261 cacggttcgt taagtagcga ctatagtttt ctattccttt tacaaaacca gtttcttcta
     1321 gcatttcaag gtcaaatttg gtgcgttgag ctaagcgctg ggcttctaaa agttgattgt
     1381 tcgcattaaa gtactgtaat cgttgctgta attctgtttg aatattagcg attgcacctt
     1441 ttaacttttg ctggggtgtt acgtagtgtg acccaggata aatagtatat gattctagcg
     1501 atgcctctat ttcgcctgtt aacgggttta tcttggtaat ccggtcgatt tcgtcaccaa
     1561 aaaaatctat gcgatacgcc gattcttcac ctgcagggaa cacatcaaca acatcaccac
     1621 gtactcggaa cgtacttctg gtaaaggcaa tatcgtttcg gtgatattgg atgtctgtta
     1681 gttggcgcaa aaatttatcg cgcaagcggc gttccccaac agcaacgcga atagccattg
     1741 tgccgtaatc gtctacagac ccaataccat aaatacaact gacactcgca acaattaaga
     1801 cgtcacggcg tgataaaagt gcactggtag cagcgtgacg taacctatct atttcttcat
     1861 taatttggct atctttttct atatatgtat cactccgagc aatatatgct tctggttgat
     1921 agtagtcaaa gtaactcaca aaataatgaa cagcattatt agggaaaaaa actttgaatt
     1981 cattatacaa ctgtgcagcc aacgtcttgt tatgagccag cactaacgtg ggtttttggg
     2041 tattttgcac gacgtttgcc attgtaaagg tcttaccaga accagtaaca ccgagcaatg
     2101 tctgatgttg cagaccctgt tggatccctt tggttagtgc agcaattgcc tttggctgat
     2161 caccagtcgg ctcgtatgag gtttgtaaat caaaagatgc catgtaaata ctagtatact
     2221 actcttagga tataaagttt tacgggttgg ggtttgtata tgttacgagc ccatcacgac
     2281 actgagagct tagcccacgt ggaatatcaa acgcatttac agcatca
//
LOCUS       DAOPLS010000117         2305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_54157_length_2305_cov_4.372000, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000117 DAOPLS010000000
VERSION     DAOPLS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54157_length_2305_cov_4.372000"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKV52_02985"
     CDS             complement(<1..332)
                     /locus_tag="PKV52_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPD99210.1"
                     /translation="MGITDLKKGAVFQMDGHPYKVLEYGQKVMGRGGSIVNVRIKSLI
                     DGKVLQKTFKGNEAIEPADISYKNVQYLYNDAQTYFFMDDDSFEQIEIRADMLEGQTG
                     YLKEGDKV"
     gene            complement(793..999)
                     /gene="rpsR"
                     /locus_tag="PKV52_02990"
     CDS             complement(793..999)
                     /gene="rpsR"
                     /locus_tag="PKV52_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPD99211.1"
                     /translation="MAKPKRKPITGAIYFDYKDVKTLQRFTNQFGQIEGRKRSGLTEM
                     QQRRLARAIKQARHIALLPFVANK"
     gene            complement(1097..1549)
                     /gene="ssb"
                     /locus_tag="PKV52_02995"
     CDS             complement(1097..1549)
                     /gene="ssb"
                     /locus_tag="PKV52_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPD99212.1"
                     /translation="MARSFNQVTLMGNLTRDPELRSTANGQSVCNFSLALNRSYKDAT
                     GNWQEATDYIDVIAWGPLGERVQQYVTKGRPVLVSGRLQNRTWEQDGQKRNKVEVVAQ
                     DVTFLGGRSEGASDTPSGSAPAPSPQKKKDVVIEDISDEPIDLSEIPF"
     gene            complement(1634..1999)
                     /gene="rpsF"
                     /locus_tag="PKV52_03000"
     CDS             complement(1634..1999)
                     /gene="rpsF"
                     /locus_tag="PKV52_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPD99213.1"
                     /translation="MNSYELAILYDPDLEIDLDKAADKVKAIVKTNGGKVTTEDNWGK
                     RKLAYNIKGHDMAVYVFYGVDMPPTSVTKVEATLNITDEVIRFLITKPDVKAIKKAES
                     IKAARAKHAKREEEAKEEK"
     gene            complement(2101..>2305)
                     /locus_tag="PKV52_03005"
     CDS             complement(2101..>2305)
                     /locus_tag="PKV52_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99214.1"
                     /translation="FGMMVISAYLDKATAPIIPAFKLSRNSGLLPEHHPTTPEKVIKS
                     ITHKRQEDYFATISKRAKERLAA"
BASE COUNT          576 a          485 c          441 g          803 t
ORIGIN      
        1 tttactttgt cgccctcttt taggtagcct gtttggccct cgagcatatc tgcacgaatt
       61 tcaatctgct caaagctgtc gtcatccata aaaaaatagg tttgggcgtc attatacaaa
      121 tattgaacat ttttgtagct aatatctgct ggctcaatag cttcgttgcc tttaaaggtc
      181 ttttgcaaca ccttgccgtc tataaggctt tttatacgca cattaacaat tgagccacca
      241 cgacccataa ctttttgccc gtattcgagt actttatatg ggtggccgtc catctgaaaa
      301 acagctccct tttttaaatc ggttattccc atttgttaga ttatagaaat ttgatgatag
      361 ataatagaat ctactaccta atttctttct cctttcttat gattaactag ttgcaaacga
      421 acctacattt tataaataga gacacattgc tcgtataggc tattcatatt tgattcgcta
      481 atataaccta tgcccctagc aatgagaatc tggttctctg gttccgtcaa taatccgttg
      541 gccatagcgt aaaattgatc attttccttg taagattttc ttccaaagcc ttcggcgatg
      601 ttagaacaaa ccgaaacaga tgctctttgc atttggctag aaaggccata ctgttcatgt
      661 ttagtaaaaa actagaagta actttgtaga tatgaattga taggtggtaa gtagattgcc
      721 atacacgcaa atcgcgaaat gactttattc tgacacttct atcggccata atctataatc
      781 ctgtatctac atttatttgt tggctacaaa aggtagcagc gcaatgtggc gagcttgctt
      841 gatagctcgg gccaatcggc gttgttgcat ttctgttaag ccagaacgct tgcggccttc
      901 tatttggcca aactggttgg taaagcgctg taaggtcttt acatctttat aatcaaaata
      961 aattgcgccg gtaattggct ttctttttgg ctttgccata aaatttctcc tcctgaatta
     1021 ttaggtttta ggttctaggt tttaggttct attgtattac tataacctgc aaccttgccc
     1081 ctttaaccta tacttattaa aatgggattt ctgataaatc aattggttcg tcgctgatgt
     1141 cttctatcac gacatctttc tttttctgtg ggcttggtgc tggggcactg ccacttggtg
     1201 tatctgatgc accttcgcta cgtccaccca aaaaagtgac gtcttgggcg acaacttcta
     1261 ctttattacg cttttggcca tcttgttccc atgtgcggtt ttgtaaacga ccgctcacaa
     1321 gtactgggcg ccctttagtg acgtattgct gtacacgctc acccaatggg ccccaagcaa
     1381 taacatctat gtaatctgta gcttcttgcc aattacctgt cgcatcttta tagctacggt
     1441 ttagtgctaa gctaaaatta cagacgctct ggccattagc ggtagaacgc agttctgggt
     1501 cacgtgtgag gttacccatg agggtgactt ggttaaaact tcttgccatt ttgataattc
     1561 tcctttgcta ctttacttaa gttattagtg agtcgttttt agtaagtagt aattactaaa
     1621 atttcggagg ttattatttt tcttctttgg cttcttcttc tcttttggcg tgtttggcac
     1681 gcgcagcctt gatgctttct gcctttttaa tagcttttac atctggcttg gttataagaa
     1741 aacgaattac ctcgtcggta atatttaagg tagcttctac cttagtaaca ctcgtaggtg
     1801 gcatatctac gccataaaaa acgtacactg ccatatcatg ccccttgatg ttataggcca
     1861 gcttacgctt gccccagttg tcttctgttg ttactttgcc gccattagtc tttacaatgg
     1921 ccttgacctt atcggctgct ttgtctagat ctatttcaag atccggatca tacaaaattg
     1981 ccagctcata gctgttcata aatagaactc cttccgttgg cttaattatc gcctacgcgc
     2041 cctattggcc gtaagctgaa gttaatagta gagctagtgt accagtttta cctctgttag
     2101 tcaagcagct aagcgctctt tggcgcgttt agatatcgtt gcaaagtaat cttcttgtct
     2161 tttatgggta attgatttaa taactttttc tggtgttgta ggatgatgct cgggtaatag
     2221 accagaattt ctagaaagtt taaatgcagg aataattgga gcagttgcct tgtctaaata
     2281 tgcgctaata accatcatgc caaag
//
LOCUS       DAOPLS010000118         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_54587_length_2292_cov_3.476531, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000118 DAOPLS010000000
VERSION     DAOPLS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54587_length_2292_cov_3.476531"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKV52_03010"
     CDS             <1..464
                     /locus_tag="PKV52_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99215.1"
                     /translation="LIGEPSNTDVDQDGIDDACDGFIDLPPTPTQPAEKELIQSESAP
                     LSQYISNEPPTSLSVATSTIPFNPVNSAPVQNTPAANTATTQDTPQQTNPTPISSQNT
                     NTQIAGVSSAVESKPLSERTATSWWVYLMVATAVFITSVTFYRLIAAKNRV"
     gene            474..1571
                     /locus_tag="PKV52_03015"
     CDS             474..1571
                     /locus_tag="PKV52_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPD99216.1"
                     /translation="MAKIVIDARELRTSTGRYVERLLHYLQKTDTANKYIVLLTPNDF
                     DTWQPSAKNFSKLSCKYKEFTFGEQLGYARMLYKLKPDLVHFAMIQQPILYFGRSVST
                     FHDLTTVRYKNPNKPAWIYWAKLPAYWLVIQIAARKNKLLITPTEYVKKDVTQFTKIS
                     PDKIITTLEAADEFKEPAKALPALLGKSFIMYVGRPQPHKNLSRLIKAFSALKKKHPS
                     LRLVLAGKKDPIHATYVDYAQQLGVAEDIIFTGFVTDGELRWLYQNCQAYIFPSLSEG
                     FGLPGLEAMLHSAPVVSSNATCLLEVYGDAAEYFDPTNTLEMTKKIDLVISDSALRKK
                     LTTKGYHHAKTFSWNRMATQTVAVYEEALGM"
     gene            1620..2156
                     /locus_tag="PKV52_03020"
     CDS             1620..2156
                     /locus_tag="PKV52_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99217.1"
                     /translation="MKKFLFSFVLLGVLTISAPAAAEQTINTGTPSSDVQQSVSGDYF
                     QKNGTNQPASNITQNAGAEVLQNTPNQRLTVVGAPADSTVSATKSDFRWVAMSLLLFA
                     VLISPAILYARSSQPSSENQANAPATNAENNDANKTDTTATVTNDSAKSTAKKLPKKK
                     SKKSTKNNPAKRRKKSKK"
BASE COUNT          740 a          508 c          450 g          594 t
ORIGIN      
        1 gcttaattgg tgaaccatca aatacagatg tagaccaaga tggtatagat gatgcatgtg
       61 atggctttat agatctgcca cctactccaa cacaaccagc agaaaaagag ctaatacaat
      121 ctgaatctgc gccgctatca caatatataa gcaatgagcc ccctacatca ctatctgtag
      181 caaccagcac gatcccattt aaccctgtaa atagtgcacc agtgcaaaac actcccgctg
      241 caaacacagc tactacacaa gataccccac agcaaactaa ccctacaccc ataagctcac
      301 aaaacaccaa tacacagatt gcaggtgtga gtagtgctgt tgaaagcaaa ccactcagcg
      361 aacgtacagc cacaagctgg tgggtgtatt tgatggttgc cactgccgtt ttcataacgt
      421 ctgttacgtt ctaccggctc atagctgcta aaaaccgcgt ataataaagc ataatggcaa
      481 aaatagtaat agatgcgcgt gaattaagaa ccagcactgg ccgctacgta gagcgtctgc
      541 tgcattattt gcaaaaaaca gataccgcca ataagtatat tgtgctgcta acaccaaatg
      601 attttgatac gtggcaacca agtgccaaaa acttttctaa gctaagttgt aaatacaaag
      661 aattcacctt tggcgaacaa ttagggtacg ccagaatgtt atacaagcta aagccagatt
      721 tagtgcattt tgcgatgatt caacaaccaa tattgtattt tggtaggtca gttagcacgt
      781 ttcacgattt aacaaccgtg cgctataaaa accccaataa accagcttgg atatattggg
      841 caaaactacc tgcttactgg ttagttatac aaattgccgc tcgtaaaaat aaattactta
      901 taacgccaac agaatatgta aaaaaagacg ttacgcagtt tacaaaaatt agtcctgata
      961 aaattattac taccctagaa gcagccgatg aatttaaaga acctgcaaaa gcgctacccg
     1021 cgttacttgg taaatctttt attatgtatg ttggccggcc acagccccac aaaaacctca
     1081 gccggcttat aaaagccttt agtgcattaa aaaagaagca tcccagctta cggttagtgc
     1141 ttgctggtaa aaaagatcct atacacgcta cgtatgtaga ctatgcccag cagttgggtg
     1201 tggcagaaga cattattttt accggttttg taaccgacgg cgagctacgc tggttgtacc
     1261 aaaactgcca agcgtacatc tttccttctt taagtgaagg gtttggctta ccagggttag
     1321 aagcgatgct gcatagtgca cccgttgtaa gcagtaacgc aacctgctta ctagaagtat
     1381 atggtgatgc agcagaatat tttgacccaa ccaatacact agaaatgact aaaaaaatag
     1441 atttagtaat tagcgatagt gccttgcgta aaaaacttac taccaagggc tatcaccacg
     1501 ctaaaacgtt tagctggaac cgcatggcta cgcaaaccgt agccgtgtac gaagaagccc
     1561 tggggatgta gctagtatat tttgttgttt tgttattttt aggtcatact attatatata
     1621 tgaaaaaatt tctatttagt tttgtgctgt taggtgtgct tactataagt gcgcctgctg
     1681 cggccgaaca aactatcaac accggcacgc catccagtga tgtacaacaa tcggtaagtg
     1741 gtgattattt tcaaaaaaat ggcactaacc aacctgccag caacattact caaaacgctg
     1801 gtgcagaggt gctccaaaac accccaaatc aacgcttaac tgttgttggt gcacctgccg
     1861 attctacggt atcggctacg aaatcagact ttcggtgggt tgccatgtcg ctgttgctat
     1921 ttgccgtttt aattagccct gctattttgt atgcgcgctc atcacaacct tctagtgaaa
     1981 accaagccaa tgcaccggct acgaatgctg aaaataacga tgcaaataaa acagatacaa
     2041 ccgcaacggt tacgaatgac tctgcaaaaa gcactgccaa gaaattacca aaaaagaaaa
     2101 gtaaaaaaag taccaaaaac aatccagcga agcgtcggaa aaaaagcaag aaataaccac
     2161 ctttttgggc gtgattactc ttatgtatgt gtactattta tgtataggct tattgcctag
     2221 tcggcaagtg cttctgggtc tgcactaggt tcttctgtgc cagctggtgg ccttataata
     2281 atgtgtctgt ca
//
LOCUS       DAOPLS010000119         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_54743_length_2287_cov_4.167563, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000119 DAOPLS010000000
VERSION     DAOPLS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54743_length_2287_cov_4.167563"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKV52_03025"
     CDS             complement(<1..204)
                     /locus_tag="PKV52_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99218.1"
                     /translation="MAEEQTTPQTIEDVKPPTITKHEANRAGSTPAQDAANILPAEEQ
                     PTETIISNKGTNIGFNNSKRMNPK"
     gene            complement(209..1006)
                     /gene="tpiA"
                     /locus_tag="PKV52_03030"
     CDS             complement(209..1006)
                     /gene="tpiA"
                     /locus_tag="PKV52_03030"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPD99219.1"
                     /translation="MATHKKTLIAGNWKMHLTVAQASLLVQRLHERTQNHKDVEVVLA
                     PTHLHLQPLSLQIDHRKFKLAAQNAYHKDEGAYTGEVSFTMLRHLVSYGLVGHSERRH
                     IFNESLDEITLKVAAAYRNGITPILCVGETKIDRLSKETNQVLHDQVVSALANVTSEE
                     VQSIVIAYEPVWAIGTGENAKPSDVASVARVIKHDITELYGTKAAERVRILYGGSVTA
                     DTASGYLRLPEIDGLLVGGASLNYHSFSDIIDSAYRVKRNINTLRDI"
     gene            complement(1049..>2287)
                     /locus_tag="PKV52_03035"
     CDS             complement(1049..>2287)
                     /locus_tag="PKV52_03035"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPD99220.1"
                     /translation="DFNVPINDLGVITDDYRIRKALPTIEHLRSSGAKVVLISHLGRP
                     TSKQDVACSLAPIAHRLDDLLHTTVLFADDCISADAKHKIKELQPGDVLLLENLRYYP
                     EEEANDPDFAKKLAKGCDVFVQEGFGVVHRAHASTDAITRYLPSVAGLLLAKEVDTIT
                     NAMHTPKKPLAIVIGGAKISDKIDLLNTFIDSADYIAVVGAMANTFLVAEDVAVGSSL
                     VEKDAVHTAKELLQKAYSRMKKERFTFYIPQDVVVAKGKENTHQTRVVDIDNHTWADI
                     SHYPKKPSKHAYSVVADEWILDIGPMTASAIKGALSQAHTAIWNGTAGMTEIRGLHGA
                     ADPFSHGTRVITEGFIGQHAGDKNVPFTIVGGGDTVGYVESISGLREQLGHVSTGGGA
                     SLELMAGHTLPGVEALQDSD"
BASE COUNT          564 a          516 c          472 g          735 t
ORIGIN      
        1 tttcggattc attctcttcg aattattgaa cccaatattt gtacctttat tagaaataat
       61 agtttctgta ggttgttctt ctgccggtaa tatatttgcc gcatcttgtg caggcgtcga
      121 acctgcccta tttgcttcgt gctttgtaat tgtcggtggc ttaacgtctt ctattgtttg
      181 gggtgtggtt tgttcttctg ccattttttt atatatctct tagggtatta atatttctct
      241 ttactctgta ggcactatca ataatatcgc taaatgaatg ataattaaga ctagcgccac
      301 caactaataa cccatctatt tctgggaggc gtaggtatcc agatgctgta tctgccgtga
      361 cgctgccacc ataaagtata cgtacccgtt cagctgcttt tgtaccatat aattctgtaa
      421 tatcgtgctt aatgacacgc gctaccgatg ctacgtcaga tggcttggcg ttttctcctg
      481 taccaattgc ccagacaggt tcatacgcaa taacaattga ttgcacttct tcactggtaa
      541 cgttcgctaa agcacttaca acctgatcat gcaatacttg gtttgtttct ttagatagcc
      601 gatctatttt tgtttcgcca acgcataaaa taggagtaat gccatttctg taagcagcag
      661 ccactttgag ggtaatttca tcgagtgact cattaaaaat atggcggcgc tctgaatgcc
      721 caaccagacc ataacttact aggtgacgta acatggtgaa cgatacttca ccagtgtagg
      781 cgccttcgtc tttatggtaa gcattttgag cagctaattt aaatttgcga tgatcaattt
      841 gcaaactcag tggctgtagg tgaagatgag taggagccag tacaacttct acgtctttat
      901 ggttttgggt acgttcgtgc agccgctgca cgagtaaact tgcttgcgca accgtaagat
      961 gcatcttcca gttcccagct atgagtgttt ttttatgtgt tgccatatat atacaatgct
     1021 tatgttttta tagtaccacg ttctgcgttt agtcgctgtc ttgtaacgct tctacaccag
     1081 ggagagtatg cccagccatg agctctaagc tcgctccacc acctgtagat acgtgtccaa
     1141 gttgctcgcg taatccactt atagattcta catagcctac cgtatcaccg cctcctacaa
     1201 tggtaaatgg tacgtttttg tcaccagcat gttggccaat gaagccttct gtgattacgc
     1261 gcgtcccgtg gctaaacggg tcagctgcgc cgtgtaagcc acgaatctct gtcatgccag
     1321 ctgtgccatt ccatatagca gtgtgcgctt gcgatagcgc accttttatt gcactcgctg
     1381 tcattgggcc tatgtctaaa atccattcat cagccacaac gctgtaggca tgttttgaag
     1441 gttttttcgg ataatgtgaa atatctgccc atgtatgatt atctatatca acaaccctcg
     1501 tctggtgggt gttttctttt ccttttgcca caacaacatc ttggggtata taaaacgtaa
     1561 agcgctcttt tttcattcta ctatatgctt tttgcaggag ttctttggcg gtatgcacag
     1621 cgtctttttc taccaaacta ctaccaacgg ctacatcttc tgcgactaaa aaggtattcg
     1681 ccatggcacc aacaaccgct atataatctg ctgaatctat aaatgtgttt aacaaatcta
     1741 ttttgtcgga tatttttgcg ccaccaatca caatggccag tggctttttg ggtgtatgca
     1801 ttgcattggt tattgtgtct acttcttttg ccagtagtaa tcctgcaacg ctcggcagat
     1861 accgagtaat agcatctgtg ctagcatgag cacgatgcac aacaccaaag ccttcttgta
     1921 caaatacatc acaacctttg gcgagctttt tagcaaagtc agggtcgttt gcttcctctt
     1981 ctgggtagta acgtaggttt tctagtagta aaacatcacc cggttgtagc tctttaattt
     2041 tgtgttttgc gtctgcgcta atacaatcat cagcaaagag cactgtggtg tgcaataaat
     2101 catcaagacg atgagcaata ggcgcaaggc tgcaggcaac gtcttgttta ctagtcggcc
     2161 taccaagatg agaaatcagc acaaccttag cgccagagct ccgtaggtgc tctatagtgg
     2221 gcaaggcttt gcgaatgcgg taatcgtctg taataacacc tagatcattg atgggcacat
     2281 taaaatc
//
LOCUS       DAOPLS010000120         2279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_55018_length_2279_cov_5.149281, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000120 DAOPLS010000000
VERSION     DAOPLS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55018_length_2279_cov_5.149281"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKV52_03040"
     CDS             complement(<1..336)
                     /locus_tag="PKV52_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPD99221.1"
                     /translation="MSKHEHRKHTRILSVGAAVQDVFLQGKIFKPHKDCDGDMIEEFE
                     LGSKNDIDGVIFSTGGGATNASVTFARQGLHAMYLGKVGDDVAGRAILDDLHKDGVDT
                     SLVGLSNNGY"
     gene            complement(443..1660)
                     /locus_tag="PKV52_03045"
     CDS             complement(443..1660)
                     /locus_tag="PKV52_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HPD99222.1"
                     /translation="MNEIICPHCKKAFTIDEAGFADIVQQVRNHEFNKELHERLALLE
                     KEKENAVKLAEQKAQNALIAESAKKDAELAKLKADAEAKITRMQAKLENTETEKKLAL
                     TDAVHAIEKQRDDLVNQLKSKDHEAQLLKASLKDKYESELKSKDEMIAYYKDMKAKLS
                     TKMVGETLEQHCETEFNKLRATAFQNAYFEKDNDVSSGSKGDYIYRDSDENGTEIISI
                     MFEMKNEGDETATKHKNEDFLRELDKDRTEKKCEYAVLVTLLESESELYNSGIVDVSY
                     KYQKMYVIRPQFFIPIITLLRNAARHSLAYKSELALVKAQNIDVTNFEGQLNDFRESF
                     GRNYRLASEKFKTAIDSIDKSIAQLQKTKDNLLRSEDNLRIANNKAEELTVKKLTRGN
                     PTMAAEFEEQNNS"
     gene            complement(1883..>2279)
                     /locus_tag="PKV52_03050"
     CDS             complement(1883..>2279)
                     /locus_tag="PKV52_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPD99223.1"
                     /translation="RGAEKFVKDTGIDTYAAAIGNLHGSYPVPKTLDIALLERIRKAL
                     PRNTNISLHGGSGTPAHFYEEAVRAGVTKINVNSDMRRAFRDTLEKVLRENPDEYAVV
                     KLMPEVYGAVQTVVETKLASYNSIGKAHY"
BASE COUNT          543 a          491 c          453 g          792 t
ORIGIN      
        1 atagccgttg ttagacagcc caacgaggct cgtgtctaca ccatctttgt gtaaatcatc
       61 cagtatcgct cgccctgcaa catcatcacc taccttaccc aaatacatcg cgtgcaagcc
      121 ttggcgggca aatgttacag aggcattggt tgcacctccg cccgtactaa atattacgcc
      181 gtcaatatcg tttttagaac cgagttcgaa ctcttctatc atatcgccat cgcagtcttt
      241 gtgcggttta aagattttac cttgcaaaaa tacatcttgc acagctgctc cgacagataa
      301 gattcgggta tgttttctat gttcgtgttt gctcataata tacagggtcc agtataccat
      361 ttatgcttat cgccgcctag cctggttggt atatgaaacg tttttaatat aggctataga
      421 ctgctacaaa caaaacgaaa cactagctat tgttttgctc ttcgaactca gctgccatcg
      481 tagggttacc acgcgtgagc ttctttactg ttaactcttc tgctttattg ttagcaatgc
      541 gtaaattatc ttctgagcgc aataagttat ctttggtttt ttgcaattga gcaatagatt
      601 tatcgatact atcgatggct gttttaaatt tctcggacgc gagcctatag tttctgccaa
      661 acgattcacg aaaatcatta agctgacctt caaaattagt gacatcaatg ttttgggcct
      721 taacaagtgc taattctgat ttatatgcaa gtgaatgtcg tgcagcgtta cgcaataagg
      781 taatgatggg tataaaaaat tgcggccgaa taacatacat tttttggtat ttgtaagaaa
      841 catccacaat gccgctatta tacaactcgc tttcgctttc tagcagcgtc acaagcacag
      901 catactcaca tttcttttca gtgcgatctt tgtcgagttc gcgcagaaaa tcctcgtttt
      961 tgtgtttcgt ggcagtctcg tcaccttcgt tcttcatttc gaacattatc gaaatgattt
     1021 cagtgccatt ttcgtcagaa tcacggtaga tgtaatcacc cttgctacca cttgaaacat
     1081 cgttatcttt ttcaaaataa gcgttctgga acgccgttgc gcggagttta ttaaattctg
     1141 tttcgcagtg ttgttccagc gtttctccca ccatcttagt agatagtttc gctttcatat
     1201 ctttatagta ggcaatcatt tcatctttgc ttttaagttc ggattcgtat ttatctttaa
     1261 gcgaggcttt taacagctgg gcttcatggt ctttgctttt tagttggttt acaagatcat
     1321 cgcgctgttt ttctatggcg tgaactgcgt ctgttagggc aagctttttt tctgtttcag
     1381 tgttttctaa ctttgcttgc atacgggtaa ttttcgcttc tgcatcggct ttaagttttg
     1441 ctagttcggc atcttttttg gcagattctg ctatgagagc attttgtgct ttttgttctg
     1501 ccagcttaac tgcattttct ttttcttttt ctagcagtgc taagcgttcg tgtagttctt
     1561 tattaaattc atgatttcgg acttgctgca caatatcagc aaaacctgct tcatctatgg
     1621 tgaaggcttt tttacaatgg gggcaaataa tctcattcac tatatactcc atttcgtgtt
     1681 actcaatgtt tgctacacaa tgtcgtggta cacaaacatt cttctatgta gtatggcata
     1741 gtttgggcgt gaatgtttta tcaatggtat cgtgtgcaac ctttaacttt tctagataaa
     1801 ctgtggcagt gggtacgagt cgcatcgtct aatcttgcac gctatatgta gcgtaccgac
     1861 gctacctgta gcgtggatgt acttaatagt gggctttgcc tatagaattg tagctcgcca
     1921 gtttggtttc tacgacggtc tgaacagcac cataaacttc tggcatgagt tttacaacgg
     1981 cgtattcatc tggattttca cgcaagacct tttctaaggt gtcacggaag gcgcggcgca
     2041 tatcgctgtt aacattaatc ttcgtaactc ctgcccgaac agcttcttcg taaaaatgag
     2101 ccggcgtgcc actgccgcca tgtaagctaa tgttagtgtt tcttggtaag gcttttctga
     2161 tacgttctag cagagcgata tctaacgttt ttggtaccgg gtaactgccg tgtaaattac
     2221 caatagcagc cgcatatgtg tctatgccag tatcttttac aaacttttcg gcaccacgt
//
LOCUS       DAOPLS010000121         2240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_56353_length_2240_cov_4.056751, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000121 DAOPLS010000000
VERSION     DAOPLS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56353_length_2240_cov_4.056751"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1994)
                     /gene="gyrB"
                     /locus_tag="PKV52_03055"
     CDS             complement(39..1994)
                     /gene="gyrB"
                     /locus_tag="PKV52_03055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPD99224.1"
                     /translation="MNAIYSEESIRTLDWKEHIRKRPGMYIGGTGRDGLHHLIKEIAD
                     NSVDEAIAGHASEVRVRLLADGGVEVSDDGRGIPIDIHPKTGKSTLETVLTILHAGGK
                     FGGGGYKVSSGLHGVGLSVVNALSNRMIAQVYKDGKTYQQTYEKGVPTSELEIIGKTD
                     KTGTTITFWPDDTIFQETNFDYEWVVDYLRHQAYLTKGVKTLVIDDIASQEYCFYFEG
                     GIQSYVRHLNIGKDVVGDKIFYVEKQIQDVGVEIALQYNDSFNETVKAFANNVFNPDG
                     GTHMVGFRAALTRVINDYARKNSLLKEKDDNLSGDDVREGLTSVILVKIPDPQFEGQT
                     KNKLGNPEIRGYVEKVMSEYFSYYLEENPAVAKGIIGKALLAARARKAARAARDSIIR
                     KGVLDGASMPGKLSDCSSKDPADSEIYLVEGDSAGGSAKSGRDSKTQAILPLRGKVLN
                     VERARLDKMLANNEIVSLIKALGVGIDEQFDISGLRYHRIIIMTDADVDGSHISTLLL
                     TFFFRYMAPVIEGGYMYLAKPPLFAIKLSATKKRYAYSDEERDSILQELVEARKAAGV
                     TIDPSDDIRKQAGVSEVQRYKGLGEMDADQLWETTMNPENRVLIQVKIEDAEKADAIF
                     NKLMGEEVDLRKNFIQSRAKFVKDLDI"
BASE COUNT          588 a          528 c          403 g          721 t
ORIGIN      
        1 ccatatctgg tgtatctttt actacatctt cgtccatatt atatatccaa atcctttaca
       61 aatttagctc gtgactgtat aaagtttttg cgcaaatcta cttcttctcc cattaactta
      121 ttaaagattg cgtctgcctt ttctgcatct tctatcttta cttgaatgag cactctgttt
      181 tctggattca tggttgtttc ccataattga tcggcgtcca tttcaccaag acctttatat
      241 ctttgcactt ctgaaacacc tgcctgtttt ctaatatcat cactaggatc aattgttacg
      301 ccagcagctt ttctcgcttc tacgagctct tgcaatatag agtcacgctc ttcatctgaa
      361 taagcatatc tcttcttggt cgcacttagt ttaatagcaa agagtggtgg ttttgctaag
      421 tacatataac caccctctat tactggtgcc atatatctga agaaaaaggt tagtagcaac
      481 gtgctaatgt gtgatccatc tacgtctgca tctgtcatga taataatgcg gtgatagcgc
      541 aagccactaa tatcaaactg ttcatctata ccaacaccaa gtgctttaat gagacttaca
      601 atttcgttgt ttgcaagcat tttgtctaat ctggctcgtt ctacattcaa cactttacct
      661 ctgagcggca aaatagcttg tgtcttacta tcacgcccac tcttggcgga cccacccgca
      721 gagtcaccct ctacgagata aatctcggaa tcggctggat ccttagaaga acaatcagat
      781 agtttacctg gcatgctagc gccatctaat acgcctttgc gaatgatact atctctggcg
      841 gcgcgggctg cttttcgggc acgcgcggca agcagcgctt tacctataat gcctttggcg
      901 acggctgggt tctcctctag atagtatgaa aagtattctg acattacttt ttctacgtaa
      961 ccacggattt cagggttccc aagtttgttc tttgtttgtc cttcaaactg aggatcaggg
     1021 atttttacta aaatcacaga tgttagacct tcacgaacgt catcaccaga taaattatcg
     1081 tctttctctt ttaggagact attcttacgt gcatagtcat ttattaccct cgtaagtgcc
     1141 gcacgaaagc ccaccatgtg tgtgcctccg tctgggttaa aaacattatt agcaaacgcc
     1201 ttaacggttt cgttaaacga atcgttatac tgcagtgcaa tttctacacc tacgtcctgt
     1261 atttgttttt cgacgtaaaa tatcttgtca ccgactacgt ctttgccaat attcaaatgg
     1321 cgaacgtaac tttgtatacc accttcaaaa taaaaacagt attcctgcga cgcaatatca
     1381 tcgataacaa gcgtcttgac ccctttggtt aagtacgcct ggtgtcgcaa ataatctaca
     1441 acccattcgt aatcaaaatt tgtttcttga aaaattgtat catcaggcca aaaagttatt
     1501 gtggtgcctg ttttatctgt tttaccaatt atctctagtt cggaagtcgg tacacctttt
     1561 tcgtacgtct gttgatacgt tttgccatct ttatagactt gggcaatcat tcggtttgat
     1621 agcgcattca cgacgctaag accaacaccg tgcaaaccac tggagacttt ataaccacca
     1681 ccaccgaatt ttccacccgc gtgcaaaatg gtaagaacag tttctagtgt acttttgcct
     1741 gttttagggt ggatgtctat tgggataccc ctcccatcat cagacacttc tacgccacca
     1801 tctgccaata atcggacgcg cacttcactc gcatgaccag caatagcttc gtctacggag
     1861 ttgtcggcaa tttcttttat taagtgatga agcccatcac gacccgtacc accaatatac
     1921 atacccggtc ttttacggat atgttccttc cagtcaaggg ttctgatact ttcttccgaa
     1981 tatattgcat tcatactccg ccaaaatttg tgcaaatata aataaaacaa caggagagat
     2041 aatatttgtt tttcaacaag gaattaacag cggggccggt taagtgcggg attgatagac
     2101 agctttgacc ataaaatgcg ttctggcgtt tttttgataa aaatgatgtg tgtcaaactt
     2161 ccattcccat tgagaattaa cacttccttc cggcaggttg acgtaaaccg tgttaatttc
     2221 agtagtacgc ttatcccggg
//
LOCUS       DAOPLS010000122         2225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_56909_length_2225_cov_3.104608, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000122 DAOPLS010000000
VERSION     DAOPLS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56909_length_2225_cov_3.104608"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKV52_03060"
     CDS             <1..358
                     /locus_tag="PKV52_03060"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD99225.1"
                     /translation="PSKSYGWELPGGGGDNQDLLTASKRELEEETGIIANNWEVLGSA
                     YVCNGFMTEKMAVYLASDLSFAGKKEDSDETFEDMRFFSREKISKMIQNGDINDCQTL
                     AGLQYYETWLQDKDTN"
     gene            362..913
                     /locus_tag="PKV52_03065"
     CDS             362..913
                     /locus_tag="PKV52_03065"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD99226.1"
                     /translation="MADWQKLSSKIVYENRWLTVYEDQVITPAGNESIYGYMRYRCDV
                     VYVVAIDAHQNTFLTLQQRYPTGKTNWECPAGFIENETVESAAKRELLEETGMQADSI
                     KIMSTVDIANGVAAIKNNVCLAKDLKKVTNKLDPVDGIIATKKVPLAKVYDMIINREI
                     VCSQSIAAFYMAGKYLESKDKEQ"
     gene            917..1495
                     /gene="rfbC"
                     /locus_tag="PKV52_03070"
     CDS             917..1495
                     /gene="rfbC"
                     /locus_tag="PKV52_03070"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPD99227.1"
                     /translation="MANLATTEFTAHQTSIPGLLFFDVTQVDDARGFFQEKYQKAKLV
                     AGGMPESFNVVQNSLSYNKQAGVTRGFPAEPWDKYISVVKGKVFGAWVDLRKGETFGN
                     VETYEINPNTAVFLPKGVANAFQTLEPDTYYLYSVNAHWSADNYEKYCFVNLKESSFG
                     IKWPINLDTAIINDRDTQHPLLKDVSPMEVEV"
     gene            1492..>2225
                     /locus_tag="PKV52_03075"
     CDS             1492..>2225
                     /locus_tag="PKV52_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD99228.1"
                     /translation="MNNAKILVTGGNGQLGNALKQYFPSAEYVDLDEFNISDPEVYKN
                     KDWSQYQAIINAAAYTNVDGAESPEGRVIAWQANANALAYMAQVAAANNLTIVHVSSD
                     YVFDGTKVPHKEDEHFSPLSVYGASKAAGDLAISTIHNHYILRTSWVVGKGKNFVKTM
                     KELAEKGVKPSVVNDQRGRLTFAEDLAGAISHLLTTKQPFGTYNFTNSGPIVSWADIA
                     KLTYEKTGHNPNDVTGVSTADYYAGK"
BASE COUNT          758 a          433 c          469 g          565 t
ORIGIN      
        1 cccgtctaaa tcttacggct gggagttacc tggcggtggc ggagacaacc aagatttatt
       61 aactgcttct aagcgcgaac tagaagaaga aactgggata attgccaata attgggaggt
      121 cttagggtcg gcatatgttt gcaacgggtt tatgacagaa aaaatggcag tatatctagc
      181 cagcgacttg agctttgccg gtaaaaaaga agattctgac gaaacatttg aagacatgcg
      241 ttttttctct cgtgaaaaaa ttagcaaaat gatacaaaat ggtgatatta atgattgcca
      301 aacccttgct ggtttgcaat attatgaaac ttggctacaa gataaggata caaactaaat
      361 aatggcagat tggcaaaaac ttagctctaa gatagtgtat gaaaaccgct ggttaacagt
      421 gtacgaagac caagtaatca cccctgccgg caacgaatca atatacggct atatgcgcta
      481 taggtgtgat gtagtgtatg ttgttgccat agatgcacat caaaacacct ttctaacttt
      541 gcagcagcgc tatccaacag gcaaaactaa ttgggaatgc cctgctggtt ttatagaaaa
      601 cgaaactgta gaatcagctg ccaaacgcga attgctagaa gaaacaggca tgcaggccga
      661 ttctataaag attatgagta cggttgatat cgcaaatggt gtagcagcaa taaaaaataa
      721 tgtttgttta gctaaagacc taaaaaaggt aactaacaag ctagatccag tagacggcat
      781 aattgctaca aaaaaagtgc ccttagctaa agtttatgac atgattataa atagagaaat
      841 tgtatgctcg caaagcatag ccgcctttta tatggccggt aagtatttag aaagtaaaga
      901 taaggagcaa tagtatatgg caaaccttgc aacaacagag ttcaccgcac atcaaacatc
      961 aatacctggc ttactatttt ttgacgtaac acaggttgat gatgctcgtg ggttttttca
     1021 agaaaaatat caaaaagcaa aactggttgc aggaggcatg cccgaatctt ttaacgttgt
     1081 acaaaatagc ctatcttaca ataagcaagc tggtgtaaca cgtggttttc ctgccgaacc
     1141 atgggataaa tacatttctg ttgtaaaagg caaagtattt ggcgcgtggg tagatttacg
     1201 caaaggagaa acgtttggca acgtagagac ttatgaaata aaccctaata cagctgtgtt
     1261 cttgcccaaa ggtgtcgcaa acgcttttca aaccctagaa cctgatactt actatttgta
     1321 tagcgtcaat gctcattgga gcgcagataa ttacgaaaaa tactgttttg ttaatttaaa
     1381 agaatcttca tttggcataa aatggccaat caatttagat acagcaataa ttaatgaccg
     1441 tgacacacaa catccactgt taaaagatgt ctcgccaatg gaggttgaag tatgaataac
     1501 gccaaaatac tagtaaccgg tggcaatggc caactgggca atgcattaaa gcaatacttt
     1561 ccgtctgcag agtatgtaga cttagacgaa tttaatatta gcgacccaga agtatataaa
     1621 aacaaagatt ggtcacagta tcaggctatt ataaatgcgg ccgcttacac taatgtagac
     1681 ggtgcagaat cgccagaagg ccgtgtaatc gcctggcagg ccaacgcaaa cgcacttgcc
     1741 tatatggcgc aagtagccgc ggccaacaac ctaaccattg tgcatgtttc tagtgactat
     1801 gtatttgatg gtacaaaagt accacacaaa gaagacgagc attttagccc attaagcgta
     1861 tatggtgcca gcaaagccgc aggtgacctt gcaatatcaa caatccacaa ccactacata
     1921 ttgcgcacta gttgggttgt gggcaaaggc aaaaacttcg taaaaaccat gaaagaacta
     1981 gcagaaaaag gcgtaaagcc cagtgtcgtt aacgaccaac gtggccgtct tacctttgca
     2041 gaagatcttg caggtgctat tagccaccta cttaccacca aacaaccttt tggtacatat
     2101 aattttacta atagcgggcc tattgtatct tgggcagata tcgccaaact tacctatgaa
     2161 aaaacaggtc ataatccaaa tgacgtaact ggtgtatcta ctgcagacta ttacgcaggc
     2221 aaaga
//
LOCUS       DAOPLS010000123         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_58030_length_2195_cov_4.288318, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000123 DAOPLS010000000
VERSION     DAOPLS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58030_length_2195_cov_4.288318"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1294)
                     /gene="glyA"
                     /locus_tag="PKV52_03080"
     CDS             complement(2..1294)
                     /gene="glyA"
                     /locus_tag="PKV52_03080"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPD99229.1"
                     /translation="MISISTLQKYLQAEQKRQREGLELIPSENYVSKDVLEALGSVFT
                     NKYSEGYPGRRYYGGQENTDKVEVYAIEAAKKLFSADHANVQPHSGAQANESVYHAWL
                     NPGDTVLAMDLSHGGHLTHGHPVTVLARQYNFVRYKVKNLETGEIDFEELEALAAQHK
                     PKIILAGFSSFPRELNYAKFAEIGQRHGAMLMADVAHIAGLIAGKALANPFDYGFHVM
                     SSTTHKTLRGPRSGIILSKGIASNPLVALDKTIENLPTLIDRTVFPGVQGGPHMNTIL
                     AKAVAFHEAQQPSFSVYAQQTIDNAKALANELMKQEVKLVTNGTDNHLLLIDVFRSLG
                     INGLEAEVALDKAGLTVNKNVIPDDTLPPFKPSGIRLGTPAITTRGFKTEHMPQLATW
                     IVAALKNRNDDKKLHALNQEVKAVATTFPLPSDSSPKT"
     gene            1361..>2195
                     /locus_tag="PKV52_03085"
     CDS             1361..>2195
                     /locus_tag="PKV52_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD99230.1"
                     /translation="MSSNNVKIGHLITKIRQERGLTQAEFARRLGTSQSAVNRIEQGK
                     QNLSLETIARISTALGKDIVTLNHPKSLSFEINGGKELHGEITMKSSKNATVAILAAS
                     LLNKGTTYIENAPRIEEVFRLLEVLESIGVHVKWIKGTDLEIKPPAKLRLDKIDAEAA
                     KKTRSIIMFIGAIMHYHNEFEIPFAGGCHLGKRTIAAHQFALEQFGVFIETQSGKYVI
                     KVGKKQPENVTMYEMGDTATENALLAAAMTKGKTTIRFAASNYMVQDLCLFLQKLGVK
                     ID"
BASE COUNT          639 a          424 c          463 g          669 t
ORIGIN      
        1 gctaggtttt tggtgagcta tcggatggta gtggaaacgt tgtagcgact gcttttactt
       61 cttggtttaa tgcatgtagc tttttgtcat catttctgtt ttttagtgca gcgactatcc
      121 aggttgctaa ttgtggcata tgttctgtct tgaatccacg cgtggtaatt gcaggtgttc
      181 ccagccgaat accgcttggt ttaaatggtg gtaatgtatc gtctggaatg acatttttat
      241 tcactgttag accagcttta tctagtgcaa cttctgcttc tagaccatta ataccaagcg
      301 aacgaaacac gtcaatgagc aacagatgat tatctgtacc attggtaacc aactttacct
      361 cttgtttcat aagctcgttc gcaagagctt tggcgttatc gatagtttgt tgtgcgtaca
      421 cggaaaaact aggctgttgg gcttcgtgaa aagcaactgc ttttgctagt atggtgttca
      481 tatggggacc accttgcacc cctggaaata ctgttctatc tattaatgta ggaagatttt
      541 ctattgtttt atctagagct acgagcggat tactggcaat acctttacta agaataattc
      601 cagatcgtgg gccgcgtaaa gttttgtggg tggtgctaga cataacgtga aagccgtagt
      661 caaacggatt ggccaatgcc ttacctgcaa taaggccggc aatgtgggcg acgtcagcca
      721 tcagcatcgc accgtgtctt tggccaattt ctgcaaattt tgcataattt aattcgcgcg
      781 ggaacgatga gaagccagct aatattattt ttggtttatg ttgtgctgca agagcttcta
      841 gttcttcaaa gtcaatttca ccagtttcta aatttttaac tttataccga acaaagttgt
      901 actgacgcgc caaaaccgtt accggatgac catgagttag atggccgcca tggctcaaat
      961 ccatagccaa aacagtatcg cctgggttca gccacgcatg gtagacagat tcgttagcct
     1021 gtgcgccaga atgtggttgt acgttagcat ggtctgctga aaataatttt ttagccgctt
     1081 ctatggcata tacttctact ttatcggtgt tttcttgccc accataataa cgacggccag
     1141 ggtagccttc ggaatattta ttagtaaaga cactacctag tgcttctaat acatctttac
     1201 ttacgtaatt ttctgaagga ataagctcta agccttctct ttgccttttt tgttctgctt
     1261 gtaaatattt ttgaagtgta ctaattgaaa tcatagctat agtatacgct tttcatatat
     1321 tttattcata ctttaccata tgtgatataa taatctcatt atgagtagta ataacgtcaa
     1381 aataggtcat ttaatcacta aaattagaca agagcgaggc ttaacacagg ctgagtttgc
     1441 acggaggctc ggcacgagcc aatcagcagt aaaccgcata gaacaaggga agcaaaatct
     1501 tagcctagaa accattgcca ggattagtac tgcacttggt aaagatatcg ttacccttaa
     1561 ccaccctaag tcacttagtt ttgaaataaa cggtggcaaa gagttacatg gcgaaattac
     1621 catgaaatcg tctaagaatg ccacagtagc gatattagct gcatcgttac ttaataaagg
     1681 tacgacatat atagaaaatg caccgcgaat tgaagaagtc tttcggctac tagaagtgct
     1741 cgaaagtatt ggtgttcacg taaagtggat taaaggcact gatttagaaa ttaaaccacc
     1801 tgcaaaactt cgtttagaca aaatagacgc cgaagctgct aaaaaaacgc gcagtatcat
     1861 tatgtttatt ggtgcaataa tgcattacca taatgaattt gaaataccgt ttgctggtgg
     1921 atgccacctt ggtaaacgca ctatcgcagc acaccagttt gctcttgaac agtttggtgt
     1981 atttatagaa acacaaagtg gtaaatatgt tattaaagtt ggcaaaaaac agccagaaaa
     2041 cgtcactatg tacgaaatgg gagacactgc gacagaaaac gccctccttg cggctgctat
     2101 gactaaaggc aaaacaacga tacggtttgc ggcatctaac tatatggtgc aagatttatg
     2161 tttattcttg caaaaattag gggtaaaaat tgatg
//
LOCUS       DAOPLS010000124         2194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_58072_length_2194_cov_3.276765, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000124 DAOPLS010000000
VERSION     DAOPLS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58072_length_2194_cov_3.276765"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKV52_03090"
     CDS             complement(<1..364)
                     /locus_tag="PKV52_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99231.1"
                     /translation="MFETSHIWHLDTEDNITIRKGAIAVDPDDMPVHIDDAKRCITVA
                     SGLQPRDFIVTAKRIGADTCYENTQSFLVTIVEADGSGIIVHGDKILQSPREWTTHRI
                     ESFWKLLEEIEPEIYSIFG"
     gene            460..1971
                     /locus_tag="PKV52_03095"
     CDS             460..1971
                     /locus_tag="PKV52_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HPD99232.1"
                     /translation="MNAPYAHYNYPPQFPVVMGTEEETALMVNVTGEWEQPYYFAAHL
                     DMFIPTSLCFVANHYSGVYLQNGGLVYAGGSCDEESSGERHIDNIERATPECVNPQQT
                     AMYIQANEHLMEDMAELYGQHTAEKFGKEAIIRIQRRVVDSQGNRKGCHDNFAYPYGD
                     NYDRGYDYRLPLAGYLATRSFLTGAGYVNQDHEFRFSQKIDGLKAVTGYSYAGYMYRT
                     VGAQGISETAQNRLEIRCSDINISTWATMMRLGGVALLMAALETPLKQDILGHLSYQS
                     DDVAYAEQNNAIPMGKDGMMCPDEQTYEVLDFQERLADMFLDDLQLYTDLPLDYYYIA
                     QELKVFCQDFRQVLANRQPLSILADRADWAAKFTKILAKDAEAGSARGYYEHARDDML
                     FDYIGITAQPDGSVVRKKGYGFKMRDNGAFKKSLHPKDIANAYYNAPLQTRANIRSTF
                     IKDYAVEECTWDSVSVLLKSGVGRRIDLSDPTRATVHKQASKELAEKAQPRDE"
BASE COUNT          681 a          438 c          465 g          610 t
ORIGIN      
        1 aaccaaaaat gctatatatt tctggttcga tttcttctaa tagcttccaa aatgactcta
       61 tacggtgcgt ggtccattct ctaggtgatt gtaaaatctt atcaccatgt acaattatgc
      121 cactgccatc agcttctact attgttacta aaaaactttg agtattttcg tagcatgtgt
      181 ctgccccgat acgtttagct gtaacgataa aatcacgtgg ctgcagccca cttgctactg
      241 ttatacatcg ctttgcatcg tctatatgca caggcatgtc atcggggtct acggcaatgg
      301 ctccctttct gatggtaatg ttatcttccg tgtctaagtg ccaaatatgg cttgtttcaa
      361 acattactat attatacaat aaaatagacc aaaattatta caatattgta atatatatta
      421 attatcttgg aactgtttat tcagccatta ctttaaacca tgaatgcacc gtacgctcac
      481 tataactatc caccgcagtt ccctgttgtc atgggtacag aagaagaaac tgctttaatg
      541 gttaacgtta ctggtgaatg ggagcagccg tactattttg ccgctcactt agatatgttt
      601 attcctacat ctttatgttt tgtagcaaac cactatagcg gtgtgtattt gcaaaatggg
      661 ggcctcgtct atgcaggagg ctcttgtgat gaagagtctt caggagagcg ccatatagat
      721 aacatagaaa gagcgacacc tgaatgcgta aacccacaac agacggctat gtatattcag
      781 gcgaatgaac atttaatgga agatatggca gagctctacg gccagcacac cgccgagaaa
      841 ttcgggaaag aagctattat tagaatacaa cgtagagtag tagattcaca gggtaataga
      901 aagggttgcc acgataattt tgcctaccca tacggggaca attacgatcg tggttacgat
      961 tacagactac ctttagctgg gtatttagct acccgctcat ttttaacggg tgccggctac
     1021 gtaaaccaag atcatgaatt tagatttagc caaaaaattg atggcttgaa ggcggtcaca
     1081 ggctattcct atgcaggata tatgtacaga acagttggcg cccaaggcat ttctgaaaca
     1141 gcccaaaaca gattagaaat acgttgtagc gacattaaca tatcaacatg ggcaaccatg
     1201 atgcgcttag gtggtgttgc attgcttatg gccgctttag aaactccgct taaacaagat
     1261 attttaggtc atttatcata tcagagtgat gatgtagcct atgctgagca aaataacgca
     1321 atcccaatgg gtaaagatgg aatgatgtgc ccagacgagc aaacctacga agtcttagac
     1381 tttcaagagc gactggcaga tatgttttta gacgacttgc aactgtatac cgatttgccc
     1441 cttgactact actatattgc ccaagaatta aaagtatttt gccaagattt tcgtcaggtc
     1501 ctcgccaata ggcaaccact aagtatttta gctgatcgtg ctgattgggc agcaaagttt
     1561 acaaaaatat tagcgaaaga tgcagaagct ggcagtgcaa gaggatatta tgagcacgct
     1621 cgtgacgata tgttatttga ctacataggt atcacagcgc agcctgatgg cagcgtggtc
     1681 cgcaaaaaag gctacggatt caagatgcgt gataatggtg catttaaaaa aagtttgcac
     1741 cctaaggata tagcgaatgc ctactataat gctccattac aaacgcgagc taatataaga
     1801 agtacattta tcaaagatta cgcagtagaa gaatgtacgt gggattctgt atctgtactt
     1861 ctaaaaagcg gtgtcggtag gcgtatagat ttatcagacc ctaccagagc aactgtacat
     1921 aaacaagcat caaaagaact cgccgaaaaa gctcaaccac gcgacgagta agcagcacat
     1981 agaattgtat ctagtaaaca caagataaac ctagtatttt ttaataaaat attgtattat
     2041 agtctagttt gcgtcaaagc gatgcacgct cctctcgtga aaggaaccta agtgaaggtt
     2101 aacgtacaaa acgtagcttt agggctgtat gaacaagggc tcatcccaca gccagctgtg
     2161 ggggatttac gccgtgaatt ctggacgaag attg
//
LOCUS       DAOPLS010000125         2191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_58173_length_2191_cov_3.117041, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000125 DAOPLS010000000
VERSION     DAOPLS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58173_length_2191_cov_3.117041"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..739
                     /locus_tag="PKV52_03100"
     CDS             239..739
                     /locus_tag="PKV52_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99233.1"
                     /translation="MFGSDNNQQQGVPTGAPITNDDIMKNGSLEQPDTVAGANNASDS
                     AVPSTAASIFPPQVSADPFGTAQPAAASDDVASASTTQPTEPGDSKLLDIKQQALHHL
                     SPLVEHLDQSAEEKFKTTMMMIQAADDKSLVPAAFEAAKAIEDDKARAQALLDIINEI
                     NYFTQQ"
     gene            complement(813..1199)
                     /locus_tag="PKV52_03105"
     CDS             complement(813..1199)
                     /locus_tag="PKV52_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99234.1"
                     /translation="MQPLSKQELQHAIQQLRDSILGQVATKNDINNAVGAMSQKMFCQ
                     ADMQRALDASRDRFMDKLGQPFRQQQASMQQIMNQLDQINRRLSGLEARIGTMHGQIQ
                     NVRTDTEAVAHRTEPVKHTMLSQMFS"
     gene            complement(1475..2044)
                     /locus_tag="PKV52_03110"
     CDS             complement(1475..2044)
                     /locus_tag="PKV52_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99235.1"
                     /translation="MAWMNRQDNSGGQQRDYLNAPDTARRPMALASVLFTLLFIAAIA
                     LLLFGAGRWAYSRFTQQETSVTTTQQSDQAQEQPPKDDAATQDASSGTTTDTTQTDGT
                     SQDTTTTDTTNTESPEQANGTPTGAGSSGATDNQTTNDASSSALANTGPATPEAVPNT
                     GSASLVGIFTVSVVLAGFAHHRYLITRKR"
BASE COUNT          557 a          522 c          474 g          638 t
ORIGIN      
        1 caagagcgtc ttgcgcgtcc ctttggtgaa tcatctttaa atcgtctaac atatatttac
       61 cctctttctt gttagtaatt ataccctata acagcagccc caccagctta cgcatacacc
      121 atgtttaact gtttcggccc acaaagtaac aggctgttta tgcaaattta tttacacgta
      181 tgctcttaat tagctatact gttatgtaag cataacgaca ataaataagg gggtggtaat
      241 gtttgggagc gataataacc aacaacaggg tgtgccaaca ggggcgccaa taacaaacga
      301 cgatattatg aaaaacggca gcctagaaca gccagatacg gttgcgggcg ccaacaatgc
      361 tagtgacagt gccgtgccat ctacggctgc gagtattttt ccaccgcaag taagtgcaga
      421 cccatttggc actgcacagc cagcggctgc atcagatgat gttgcaagcg cgtctacgac
      481 tcagccaaca gaacctggcg atagtaaact actagacatt aaacaacaag cgcttcacca
      541 tctttcacca ctcgtagaac acctagacca gtctgccgaa gaaaagttta agactacgat
      601 gatgatgata caggcagccg atgataaatc actagttccc gccgccttcg aggcagcaaa
      661 agccatagaa gatgacaaag cacgtgctca agccttgcta gatatcatca atgaaattaa
      721 ctactttacc caacagtaaa gtactaaacg cccccaacca aaatactccc tacattgtag
      781 gtagtatttt tatagccccg cgcgatactt gtttatgaaa acatctggct taacattgtg
      841 tgctttactg gctctgttcg gtgcgcaact gcttctgtat cggtgcgcac gttttggatc
      901 tgcccatgca tagtgcctat tcgcgcctct agcccactta accggcggtt aatttggtca
      961 agctgattca ttatttgttg catacttgct tgctgttgcc gaaacggctg gccgagctta
     1021 tccataaacc gatcgcgcga tgcgtctaac gcgcgttgca tgtcagcttg gcaaaacatc
     1081 ttctgagaca tcgctccaac cgcgttattg atatcgtttt ttgttgctac ttgccccaaa
     1141 atgctgtcgc gcagctgctg aatggcatgc tgtagctctt gtttacttag tggttgcata
     1201 tctaccccct tatacacgtt agtaccattt ggttttacgc gtttgtttgg atatttggca
     1261 acgtaccaaa aagctaatag atacttatta ttaagctacc tccatactgt tagctttatg
     1321 ttcccaatgt ttgtatcgtt atccgttaac caggctggcg attacggtat tatcgactgc
     1381 tatttattgt gagcttactg ccacacgtat aaaacagctt tcttacctac attataccac
     1441 gcagtagcaa atggtacgac gctaattagc agctttatcg cttacgcgta attaaatacc
     1501 tatggtgggc aaaaccggcc aatacgacac taacggtaaa aataccaacc agtgatgcag
     1561 acccagtgtt gggtacggct tctggtgtag ctggccctgt gttagccaac gctgaagagc
     1621 ttgcatcgtt cgttgtttgg ttgtctgtag cgccactact tcctgcaccc gtgggagttc
     1681 cattagcttg ttcaggtgat tcggtattcg tcgtatctgt tgtcgtagtg tcctgtgaag
     1741 taccatcagt ctgggtggta tcggttgttg taccactgct tgcatcttgg gtagctgcat
     1801 cgtcttttgg tggttgttct tgagcttggt ctgattgttg agtcgtggtt acgctagttt
     1861 cttgttgagt aaatctacta tacgcccaac ggccggcgcc gaacagcaat aacgctatcg
     1921 ccgctatgaa aagtagcgta aagagtactg acgccaacgc cataggtcgc cgtgcggtat
     1981 ctggtgcatt taaataatct cgttgttgcc caccgctatt gtcttgacga ttcatccagg
     2041 ccatagcttc tccttattct tgtaaggtat tgtacactag ttatagttgc aatttcaaat
     2101 aattgttgca taactaaatg cttatgcaaa agaccccccg tgatgtacga ggggtctttc
     2161 ttgtgtagca actcttaacc gagcaagtgc t
//
LOCUS       DAOPLS010000126         2174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_58797_length_2174_cov_4.641812, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000126 DAOPLS010000000
VERSION     DAOPLS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58797_length_2174_cov_4.641812"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKV52_03115"
     CDS             complement(<1..685)
                     /locus_tag="PKV52_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPD99236.1"
                     /translation="MSNEIVSHKDNRAEDITEFFNSQEQLKDSTQRRDYFEHLDENEF
                     LDLVQQTAGLVRTGKVENQHFDGETVGLMTHEVPDQREKEQLLRDTWTVAKEFLSDAE
                     IPDEDALDYAALTVAGGLLYAHPFMDGNGRTSRTLSYMIARGNGDKQELHDILAETGG
                     GRNWQVAPVPLVTSGQSVFEGKQPKSIEWEDTFVGEAEDALGGAIANSRYKDAIIRKF
                     IEKYGELAGE"
     gene            1045..1632
                     /locus_tag="PKV52_03120"
     CDS             1045..1632
                     /locus_tag="PKV52_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99237.1"
                     /translation="MNPNDPSQQQPPAQQWNYTSGNLVEDGDQSSPESFQQYDAAQNG
                     SIVAWSASEFVQHDKSAKWYFTLVGGALVLAAVVFVFTRQLLSVAVVFVMAALFGVYG
                     AAKPRTLQYEITPSGIQIGNKLYSFNAIKSFSVINEEGIPYIQLLLQKKLSVPLTVYV
                     SPDQIDLVVDALGKFVPYDQKRRDFADKLSSRLRF"
     gene            1632..1868
                     /locus_tag="PKV52_03125"
     CDS             1632..1868
                     /locus_tag="PKV52_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99238.1"
                     /translation="MSSTAKGLLWIFSGPTLLVAMAMLQVIIRLFFSAEDNVIASLIN
                     IVSWLAGTVAVLLFIVGPVVGIIILAKKQNNPGK"
     gene            1903..1979
                     /locus_tag="PKV52_03130"
     tRNA            1903..1979
                     /locus_tag="PKV52_03130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1937..1939,aa:Arg,seq:gcg)
BASE COUNT          559 a          483 c          472 g          660 t
ORIGIN      
        1 gctctccggc taactcaccg tatttttcga taaactttct aataatcgcg tctttatatc
       61 tactgttggc aatagcacca ccgagcgcat cttctgcctc gccaacaaat gtatcttccc
      121 attcaatcga tttcggttgc ttgccctcaa acaccgactg accgctcgtc actagaggca
      181 ctggtgcgac ttgccagtta cggccaccgc cagtttcggc aagtatgtcg tgcaactcct
      241 gcttatcacc gttaccacga gcaatcatgt acgagagtgt tcgactagtg cgaccgttgc
      301 catccataaa tgggtgtgca tacagtaagc caccagcaac cgtaagtgca gcataatcca
      361 gtgcatcttc atcgggaatt tcggcatcac tcaaaaactc ttttgctact gtccatgtat
      421 cgcgcaaaag ttgctctttt tcccgctggt cgggcacttc atgcgtcatt aacccgaccg
      481 tttcgccatc aaaatgctgg ttttctacct taccagttcg tacaagtccc gcagtttgtt
      541 ggacaagatc aaggaactcg ttttcatcta ggtgctcaaa gtaatcacga cgctgtgtag
      601 agtctttgag ttgctcttgg gagttgaaaa actctgtaat atcttctgcg cggttgtctt
      661 tatgggaaac tatctcgttc gacatggttt agctttcgat tttcttgtat ttttatttta
      721 tagttagact gcttaagata ctagcacatt attgctctta tgtcaatgac cggttgtcat
      781 agtagagcgt ctaaaacccc aaaaactcct ctcaatttcc aaaattcggc gggggaaatc
      841 tttcagaaaa tcaggcgagg agttttttgg ggttttcggc cagtgcgtca ggcatttgcg
      901 ccaaaatggg ttctagctcg gttgtaaaac tgcgccataa aatgagcccc agcttgtctg
      961 gggtatattt tatggcaaag tgaagtatgc tcgaacgcca aaatgtattc aattatagcc
     1021 attagcgtgt aaaataagca ttagatgaac cctaatgacc cgtcgcaaca acaaccccct
     1081 gctcagcaat ggaactacac atctggcaat ttagtagaag atggtgacca atcatcccca
     1141 gaatctttcc agcagtatga cgctgctcaa aatggttcaa ttgtagcctg gagtgcgtct
     1201 gaatttgtac aacacgataa aagcgctaaa tggtacttta ctttagttgg tggcgctctt
     1261 gtactggctg cagtagtgtt tgtgtttaca cggcaactat tatcggtagc tgttgtcttt
     1321 gttatggcag ccttgtttgg tgtgtacgga gccgctaagc caagaacttt acagtacgaa
     1381 attacaccaa gtggcataca aattggcaac aaattatatt cgtttaatgc aataaagagt
     1441 ttttctgtta ttaacgaaga aggcataccg tatatacagc ttttattaca aaagaaatta
     1501 agtgtgcccc ttacggtata tgtttcccca gatcagattg atctggttgt agatgcgctc
     1561 ggtaaatttg taccatacga ccaaaaaaga cgtgattttg ctgacaaact aagtagtaga
     1621 ctgcgctttt gatgagcagc accgccaaag ggttactgtg gattttttct gggcctacgt
     1681 tacttgtagc catggccatg ttacaagtaa ttattagatt attctttagt gcagaagata
     1741 atgttatagc ttcacttata aacattgtaa gttggttggc aggcacagta gcagtattgt
     1801 tatttattgt tggaccagtt gtgggtatta ttatccttgc taaaaagcaa aataaccctg
     1861 gtaagtaacc gtttaatctg gtatagtaac cattgcttat aagcacccgt agctcagctg
     1921 gatagagcgt ctgtttgcgg tacaggaggt cgtgcgttcg aatcgcgccg ggtgtaccat
     1981 gtaaaaaccc ctagtttgtc tagggctttt tatattgctt cacttatctg ttggtaaagg
     2041 gggattcggc cacaatccta ctgccaatca attggcaatc ggtttcggcc ccgcctcaac
     2101 tctggcacat tcgtgcgtag ggtttccggc cttgcaaact gccactggca gttttcaccc
     2161 tatcgggttc gaat
//
LOCUS       DAOPLS010000127         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_59537_length_2155_cov_4.789524, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000127 DAOPLS010000000
VERSION     DAOPLS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59537_length_2155_cov_4.789524"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..474
                     /locus_tag="PKV52_03135"
     CDS             256..474
                     /locus_tag="PKV52_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99239.1"
                     /translation="MANRENTFSPINDIARELAEATLYVPAKPLLEKGLGSELPSRCE
                     VLGRAAYNAATLPIALAAATIIRGGIKL"
     gene            546..2075
                     /locus_tag="PKV52_03140"
     CDS             546..2075
                     /locus_tag="PKV52_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99240.1"
                     /translation="MTNTYDIDNSANYHASVDIMHDYLHPYHDMRMRAMAARFEDGQH
                     KRLERVQESTGEWIERVFLDQEIDYGTYDKSTVESKMYALADYLVVHHSDDITNEEIE
                     ATRLFIESTFGVYEEVMSDTSDASNVRSILSYAFLVPGRASRKNQDYGAESELIIPIL
                     RYVPNKYRSLFIHGVPPFILDFYEEDDDGDRGAVVCALVSPEDMFPEKADYVDETEYT
                     TAFMTAGWQAIQGVNNATEFARRLGADVAGFGAVLPRITDYGARIDNKGIFTTTGHAG
                     TIVLIFQTINVAIEQGIIDEHAPRHLAIAGLGKIGASIARLAPSYYPDAKITLYDSRE
                     PLTLTIAEEMAQDGANVHIAQSMEELLSSSSVAISAITSQLTQEEIAASKEGLLIIDD
                     SQPACIDPDIASRYGATVAWVVGMHESGTRRIQWGYGTFADEHNDLFGCEIETSILSR
                     YRKDLAGKGYSKEEIDTKTREIALTGPVTPEKVIVWRQLFQEYDIKPARLQSFGKYVI
                     T"
BASE COUNT          671 a          394 c          477 g          613 t
ORIGIN      
        1 ccgtgcagtt ggttctggta caaagcgtcg tcgcaaaata ggtgatatgc ggccaagttc
       61 gtctcggcca catcgcacac gatgaccaat catttatcta taaataggcc tgagcacgcc
      121 tatggtagcg attaaatcgt catcacaaaa aactgctcaa cactctgtat ttttacgtgt
      181 agaaacattg ctttcattaa aataaatgct atactatata tataatcaaa tagcaatatt
      241 aataaggacg taaacatggc aaatagagag aatacatttt cacctatcaa tgatatagct
      301 cgagagttag cagaagctac actttatgtc ccagctaaac cattactaga aaagggtctt
      361 ggcagtgaat taccgagccg ctgtgaggtg cttggtcgtg cagcatataa tgctgcaacg
      421 ttaccaatcg cgttagcggc agctacaatt atccgtggtg gtattaaact gtagtatctg
      481 caatgtgcct cgtctatgat ggcttaatca tgtattagta ctgcaagcaa gcgaaacaaa
      541 taaatatgac aaatacatac gatatagata attctgctaa ttaccatgca tcggtagaca
      601 ttatgcacga ttatttgcat ccttatcatg atatgcgaat gcgtgcgatg gctgcgcgtt
      661 ttgaggatgg tcaacacaag cgtctagaaa gagtccagga atcgacagga gaatggatag
      721 agcgtgtgtt tctagaccaa gaaattgatt atggtacgta tgataagtca acagtcgaat
      781 ctaaaatgta tgcacttgct gattatttag tcgtacatca ttcagatgac attacaaacg
      841 aagaaataga agccacaaga ctttttattg agtctacgtt tggagtatat gaagaagtta
      901 tgtcagacac gtcagatgcg agtaatgtgc gatcaattct ttcatacgcg tttctggtgc
      961 caggtagagc ttctcgcaag aatcaggatt atggtgctga atcagaatta atcattccaa
     1021 ttcttcgtta tgtacctaat aaatatcgga gtttatttat tcacggtgtg ccaccgttca
     1081 ttttagattt ttatgaagaa gatgacgatg gtgatcgtgg tgctgtcgtc tgtgctttag
     1141 tttcaccaga agatatgttt cctgaaaaag cagattatgt tgacgagaca gaatacacaa
     1201 cagcttttat gactgccggt tggcaagcaa tacaaggtgt taataatgct actgaatttg
     1261 ccagacgttt gggtgcagat gtagcagggt ttggggcggt tttgccgcgc attacagatt
     1321 atggtgctcg cattgacaat aaaggtattt ttactaccac ggggcatgca ggaacaatag
     1381 ttttaatttt tcaaacgata aacgtagcaa tagaacaggg aataatagat gagcatgcac
     1441 ctcgtcattt agcaattgca gggttgggga agatcggtgc ttctatagct cgcttggcac
     1501 caagctatta tccagacgca aagataacac tctatgacag cagagaaccg ctgacactta
     1561 caatcgcaga agaaatggcc caagacggag ctaatgtgca tattgctcaa tcaatggaag
     1621 aattgctttc tagtagctcg gttgctatat ccgcaattac ttcgcaactt actcaagaag
     1681 aaattgccgc cagcaaagaa ggcctcctta ttattgatga tagccaaccc gcatgtattg
     1741 acccagatat agcatcacgc tatggggcga ctgtagcctg ggtagttggt atgcatgaaa
     1801 gtggtactcg tagaatacag tgggggtatg gtacttttgc agatgagcac aacgatttat
     1861 ttggctgcga gatagaaacg tctattttat ctcgataccg taaagatttg gcgggtaaag
     1921 gatatagtaa agaagagatt gatacaaaaa ctagagaaat tgcattaaca ggcccagtca
     1981 caccagaaaa agtaattgta tggcgtcagc tttttcaaga atatgatata aaacctgcgc
     2041 gtttacaaag ctttggtaag tacgtgatta cataaccgat tatagcattt agcttaacgt
     2101 aactgcgtag tacaataata cttatgctac ttgtcgcttt tagtttattg gcaat
//
LOCUS       DAOPLS010000128         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_59637_length_2153_cov_3.192564, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000128 DAOPLS010000000
VERSION     DAOPLS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59637_length_2153_cov_3.192564"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2153
                     /gene="polA"
                     /locus_tag="PKV52_03145"
     CDS             <1..>2153
                     /gene="polA"
                     /locus_tag="PKV52_03145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPD99241.1"
                     /translation="DIMAGLAKKATAQGIETVLVTSDLDALQCVDELVKVYALKKGLS
                     NIEEFHPESFTAKYGLKTSQFLDLKALQGDSSDNIPGVPGVGEKTATSLLQKYETLDG
                     VYENIDLINGSVHDKLVAGKDSAYMSKELARLYIDAPIELDLHATDVSKFDAPRLKGV
                     LEKLEFRSLIRQMPELADVVSDNADANFEIRALPYVKLADFVWQKQLCIVGFFTDIHN
                     TNCQALVIANDTHITILDEPHKQLNGVVKKQLEGCVVSGYDTKRVVSHLLQNGVHTSV
                     EHDVKVAAFVLNSLQSPFDLHAISQRELGIDIPNLQDIPPEDIGVYAADIVGAILELT
                     VRQNEQLSSIGALHRVVQTMEWPMIPILASMECEGIQLDIPYLRVMQREFEDIISDLE
                     QTIYGFAGQEFNISSPQQLAEVLFETLGLPAQGIKKGKTGYSTAASELAKLVGYHPIV
                     PVISKYREYTKLKSTYVDTLPLQVADDGRIHTTFHLTIAQTGRLSSSDPNLQNIPVRS
                     EIGKKIRHAFVAKQGHSFISADYSQFELRLAAVLAGDTDMIEAFNSGADIHTRTAAEV
                     YGVALDDVTAEMRSNAKTINFGVLYGMSPHGLSVATGMTMNDAKDFIDKYFAARQPLV
                     SYIESLKKKAKEDGYVETLFGRRRPTPDVTSSNFIVRMSAERQAVNMPIQGTEADIMK
                     LAMIKLENALPKTAKQLLQIHDSILVEVLDNDIGI"
BASE COUNT          680 a          421 c          505 g          547 t
ORIGIN      
        1 cgatataatg gcagggctcg caaaaaaagc aactgcgcag ggtatagaaa cggtgcttgt
       61 aacgagcgat ctagacgcct tgcagtgtgt tgacgagcta gtgaaagtat atgcactcaa
      121 aaaaggctta agtaacatag aagaatttca cccagaaagc tttacggcaa aatatggtct
      181 caaaacaagc cagttccttg accttaaagc actacagggt gactcttctg ataatattcc
      241 aggcgttcct ggcgtgggtg aaaagacggc aactagtttg ctccagaaat atgaaacttt
      301 agatggtgta tacgaaaata tcgatcttat taacgggagt gtgcacgata aacttgtggc
      361 aggcaaagac agcgcataca tgagcaaaga gcttgcacgc ttgtatatcg acgcaccaat
      421 tgagttagac ttgcatgcca cagacgtaag caagtttgat gccccgcgct taaaaggcgt
      481 gctagaaaag ctagaattta gatcgttgat tcgccaaatg ccagaactcg ctgatgttgt
      541 gtctgataat gcagatgcca actttgaaat tcgggcccta ccatacgtga aactcgccga
      601 ctttgtatgg caaaaacaac tgtgtattgt agggtttttc accgatattc ataataccaa
      661 ctgccaagca ctcgtgattg ctaacgacac gcacattacc atactagacg aaccacacaa
      721 gcaactaaat ggagtagtaa aaaaacaatt agaggggtgc gttgtgagtg gttacgacac
      781 caagcgtgtg gtaagtcatt tgcttcaaaa tggtgtacat acgtccgtag agcacgacgt
      841 gaaagtggca gcatttgttt tgaattcttt gcaatcgcca tttgatttgc atgcgatatc
      901 acaacgggag cttggtatag atataccgaa tttgcaagat attccaccag aagatatagg
      961 agtatatgct gctgatattg ttggtgcaat acttgaactt acggtgcgcc aaaacgaaca
     1021 acttagtagc ataggcgcac tgcatagagt ggtacaaact atggaatggc cgatgatacc
     1081 aattttagct tctatggaat gcgaagggat tcagctagac ataccatacc ttcgtgttat
     1141 gcagcgggag tttgaagata tcattagtga tctagaacag acgatatatg ggtttgccgg
     1201 tcaagagttt aatattagta gcccgcaaca gcttgcagaa gtactatttg aaacacttgg
     1261 tttacccgcg caaggtataa aaaagggcaa aactggctat agtacagctg caagtgagct
     1321 ggcaaagctc gtcggttacc accctatagt gcctgtgatt agcaaatacc gtgaatacac
     1381 taagctaaag agtacatacg tagatacatt accgctacaa gttgcagatg atggtcgtat
     1441 ccatactacg tttcatttga cgattgcaca aactggtagg ctttcgagct ctgaccctaa
     1501 tttacaaaat attccggtac ggagcgaaat aggtaaaaaa attcgtcatg cttttgtagc
     1561 gaaacaaggg catagtttta ttagtgcaga ttacagccag tttgaactcc gtttggctgc
     1621 tgttttagcc ggcgacacag acatgataga agcgtttaat agcggtgcag atatacatac
     1681 cagaactgcc gcagaagtgt acggtgttgc attagatgat gtaacagcag aaatgcggag
     1741 taatgcaaaa acgattaact ttggtgtttt gtacggtatg agcccacacg gactaagtgt
     1801 ggcaacgggc atgactatga atgatgccaa ggactttata gataaatatt ttgctgctcg
     1861 gcagccactc gtaagctata ttgaaagcct taagaaaaag gcaaaagaag acggatatgt
     1921 agagacgttg tttggccgcc gccggccaac accagatgta acatcgagca actttattgt
     1981 tcggatgagc gccgagcgcc aagcggtgaa tatgccaatt caaggtacag aagcagatat
     2041 tatgaaactc gccatgatta aactagaaaa cgctttgcct aaaaccgcca aacagctctt
     2101 gcaaatacat gacagtattt tagtagaagt tcttgataac gatattggta tag
//
LOCUS       DAOPLS010000129         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_59882_length_2146_cov_3.323769, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000129 DAOPLS010000000
VERSION     DAOPLS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59882_length_2146_cov_3.323769"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKV52_03150"
     CDS             complement(<1..730)
                     /locus_tag="PKV52_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99242.1"
                     /translation="MATEPIILVCSSDSSIKTYSVFRRGNELANVDIVHGSGEYRFHL
                     RHKETVFDTQFAGGDRHECLSQVLAILIEQGVIDSAQDIAGVGIQISAPGGYFLQDRP
                     INDYEITILEQLKIASPTQLEEQLKEIYSIKQLLPSAKIIGVSDSAFHVTKPDYAWNY
                     AISLEDADTYDIKRFGYNGIALESVRMQLKGSQYATLNKVVVCYLGNQSSATALFNRK
                     SIDTTMGFSNSEGMMMATQAGSLSY"
     gene            complement(924..1646)
                     /locus_tag="PKV52_03155"
     CDS             complement(924..1646)
                     /locus_tag="PKV52_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPD99243.1"
                     /translation="MTNAEQFLGHYIAIERYLRRAYGSQGQYETFLQLVTKAERQQSV
                     IRYYAADLREYGELRNAIIHNRTPEDNAIIAEPHSFVVERMAHIRNMIEHPIKIADVM
                     TTPVYIAKLEDYVYETAQKMFQNVYTHVPVYDGDTFVGVLSESAILRWIAQRVVKNRT
                     LTTEHTISELTTWLDQSGNKFNDYEFVPHTMVVLDVKKRFEQALQEGRRLGAIFVTKS
                     GKSSEPIAGMVTAWDLPRLTLQ"
     gene            complement(1717..>2146)
                     /locus_tag="PKV52_03160"
     CDS             complement(1717..>2146)
                     /locus_tag="PKV52_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD99244.1"
                     /translation="IMSSIVKFDPFAEMQSLQKQFFGDDWFTPFKGLQMPTTDIYTEG
                     DKQLTVEAHLPNFDEKDVEVRADNGVLEIQAEKHEKEEDKKKKYVVRESSSSFYRRIH
                     LPQVANEDKIEAHFDKGVLKVVVPFKALPEPKKIAIKKSK"
BASE COUNT          557 a          470 c          376 g          743 t
ORIGIN      
        1 tataagaaag tgaacccgct tgtgtcgcca tcatcatacc ttcgctattt gaaaaaccca
       61 tcgtggtatc tatactcttt ctgttaaaca acgccgtggc gctactttgg ttgcctaagt
      121 agcaaaccac cactttattg agtgttgcat actgagaacc ttttagttgc atacgcaccg
      181 attctagtgc aataccgtta taaccaaacc gtttaatatc atatgtgtct gcgtcttcga
      241 gcgatatcgc gtaattccat gcatagtcag gttttgtaac gtgaaacgca ctatcagaca
      301 cgccaattat ctttgcgcta ggtaatagct gcttgatgct gtatatttct tttagttgct
      361 cttccagctg cgtcggagag gctattttta gctgttctaa tatagtaatc tcatagtcgt
      421 ttatgggtct gtcctgtaag aaatagccgc ctggtgcgct tatttgtatt ccaacaccag
      481 caatatcttg ggctgaatca ataacgccct gttcaatcag tatagcgagc acctggctaa
      541 ggcactcatg cctatcacca cctgcaaact gtgtgtcaaa taccgtctct ttatggcgaa
      601 gatgaaacct atactcacca ctaccatgca caatatctac atttgctaat tcgtttcctc
      661 ttctaaatac gctatatgtt tttatagagc tatcgctgct acaaacaagt attattggtt
      721 ctgttgccat tgattacttt ctattacagc taggtgtgtc gcattcgtag atacaacaaa
      781 agtttcacta gtttgcaatg tattttccac ctatatctat attagttttc actaccagta
      841 tacactgctt atgcttggtt gctaaggcta aataatgatg attctgccac aaagcaacca
      901 gttactacat tgttaggcat tcattattgt agtgttagtc ttggcaagtc ccacgccgtc
      961 accatgccgg ctattggttc tgatgatttg ccagatttag tcacaaaaat tgcacccaat
     1021 cgcctaccct cttgtaaagc ttgctcaaaa cgttttttca catctagtac gaccatcgtg
     1081 tggggcacaa attcataatc attgaattta tttccagact ggtcaagcca agtagtcagc
     1141 tcgcttatag tgtgctcagt tgtcaatgtt cgattcttca cgactcgctg tgctatccat
     1201 cgtagtattg cactttcact cagcactccg acaaatgtat caccatcgta tacgggtaca
     1261 tgagtgtata cattctggaa cattttttgg gctgtttcgt aaacatagtc ttccaatttt
     1321 gctatataaa caggtgttgt catgacgtca gctattttga ttggatgttc aatcatattt
     1381 cgtatatgtg ccatgcgctc aaccacaaag ctatgcggtt cggcaataat agcgttatct
     1441 tctggggtac gattatggat aatggcattt ctgagttcac catattcacg caaatctgca
     1501 gcgtaatagc gtatcaccga ttgttgtctt tctgctttag tgactaattg caaaaaagtt
     1561 tcgtattgtc cctgcgaacc atatgcgcga cgtaaatatc gttcgatggc aatatagtgg
     1621 cctaaaaact gttctgcatt tgtcattatt atattaacta tagcaaatac cacccttact
     1681 tgggtggtat tgtgttataa gctagctggt ttgtacttat ttagatttct taattgcaat
     1741 ctttttaggc tctggcaatg ccttaaatgg taccaccacc ttaagcacac ctttatcaaa
     1801 atgtgcttct atcttatctt cgttcgctac ttgtggtaaa tgtattcgcc tgtaaaaact
     1861 gctgctactt tctctcacga catatttctt tttcttatct tcttcttttt catgcttttc
     1921 agcttgtatt tccaaaacac cgttatctgc ccgtacttct acatcttttt cgtcaaaatt
     1981 tggcagatgt gcttctaccg taagttgttt gtcgccttct gtataaatat ctgtggtagg
     2041 catttgtaaa cccttaaaag gtgtaaacca atcatcacca aaaaattgtt tttgtaaaga
     2101 ttgcatttct gcaaacggat caaattttac tatactgctc ataata
//
LOCUS       DAOPLS010000130         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_59885_length_2146_cov_3.079866, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000130 DAOPLS010000000
VERSION     DAOPLS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59885_length_2146_cov_3.079866"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..513
                     /gene="gatC"
                     /locus_tag="PKV52_03165"
     CDS             220..513
                     /gene="gatC"
                     /locus_tag="PKV52_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPD99245.1"
                     /translation="MSTFSDEDIIKLAKLSKLQLTKKEVAQFKEELTAIVGYVERLQD
                     VDITGLEPTSQVTGLTNVVRKDVEIDYGVSSDALLANAPAVKDHQFKVKRMVG"
     gene            510..1937
                     /gene="gatA"
                     /locus_tag="PKV52_03170"
     CDS             510..1937
                     /gene="gatA"
                     /locus_tag="PKV52_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPD99246.1"
                     /translation="MMFNQWLPIRELVASVQSGKTSAVSLVTEAIKRAKATKDYNAIL
                     SIPEERALKRASQIDADIKAGKSVGDLAGIPFVAKDNFLTLGTPTTAASHILEPFEAP
                     FQATAIQKLEAAGAIVIAKANLDAFAHGSSTENSAFGPTKNPIDTSRVPGGSSGGSGA
                     AVALNIAPFALGTDTGGSIRLPASFCGIVGLKPTYGLVSRFGVVAMASSTDVIGPLTR
                     TSEDAALVLDVMSGRDPLDGTTIQRDTTSYTKLDSSLKGKKIGVIKEFYADGIDSDIK
                     NATLATIKKLESAGAEIKQVSLPSIDLALACYYIIVPAEVSSNLSRYDGQRYGLAASD
                     AQDLEESYEIARERGFEAENKRRIMIGTYVLSSGYYDAYYKRAQTVRTKLINEFAEVF
                     TSVDFLVGPTAPFTAFKLGQNVGDPLQMYLADIMTVAANLVGIPAISIPAGEVNKLPI
                     GIQFMAPQLHDRQLLGIAQATEKLL"
     gene            1934..>2146
                     /locus_tag="PKV52_03175"
     CDS             1934..>2146
                     /locus_tag="PKV52_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99247.1"
                     /translation="MSIGVIIGIIVVAVVILVLLNLVAKPSTKGIDKAHFINEWNDIV
                     ALSKDAKSRPLSIVHADKLLDEALKGL"
BASE COUNT          654 a          404 c          500 g          588 t
ORIGIN      
        1 aatatcaaat agttatatga ttcatgattg ctatgatgta gctgatagta tgttttgctc
       61 gcatatatcg tctaagcgtt tttgtatagc taacatgcaa tacacaaaag aggagtatat
      121 taaaatacgc gaaatggtta ttaaatggat attatcataa gttagcaagt gtttatttga
      181 caatattaaa tacctacatg aaactttata cttaatacaa tgagtacatt tagtgacgaa
      241 gacattatta agctggcaaa actaagtaag ctgcaactaa ccaaaaaaga ggtagcacaa
      301 tttaaagaag agttgacggc tatagtaggc tatgttgaac gcctgcaaga tgttgatatt
      361 acagggctag aaccaaccag tcaggtgact ggtcttacca atgttgtgcg aaaagacgta
      421 gaaatagact atggtgtaag tagcgacgct ttactggcca atgcgccagc ggtaaaagac
      481 catcagttta aggtaaaaag gatggtggga tgatgtttaa tcagtggttg ccgatacgtg
      541 aattggtagc ttctgtacag tctggcaaaa cgtctgctgt tagccttgtt acagaggcta
      601 ttaaaagagc caaggctact aaagactaca atgctatttt atctatacca gaagaacggg
      661 cgttgaagcg tgccagccaa atagatgcag acataaaagc aggtaaatct gttggcgatc
      721 ttgcgggtat accgtttgtg gcaaaagata attttttaac gcttggtaca ccaacgactg
      781 cagcgagcca catactagaa ccgtttgaag caccgtttca ggctacagcg atacaaaagc
      841 tagaagccgc aggcgcaata gttattgcca aagctaacct tgatgcattt gcgcatggtt
      901 ctagcacaga aaacagcgcg tttggtccaa ccaaaaaccc tatagacacg agccgggtgc
      961 ccggtgggtc tagcggtggt tctggtgctg cggttgcgct caacattgcc ccctttgcac
     1021 taggcacaga tacaggcggc tctatacgtc tgccagctag tttttgtgga attgttggtt
     1081 taaaacctac ctatgggttg gtgtctagat ttggtgtggt agcaatggca agttctactg
     1141 acgtcattgg tcctcttaca agaacaagcg aggatgcagc actggtactc gacgtcatga
     1201 gcggacgtga cccgcttgat ggtacaacaa tacagcgaga caccacaagt tacactaagc
     1261 tagacagttc actcaagggt aaaaagatcg gtgtaattaa agaattttat gctgacggta
     1321 tagattcgga tattaaaaat gcgacactcg ctactataaa aaaactagaa tctgctggtg
     1381 cagagatcaa acaggtatca ctaccttcta ttgatcttgc tctagcgtgt tattacatta
     1441 ttgttccggc agaagtgagc agtaatttga gccgttatga tggccagcgc tatggtttag
     1501 ctgcatcaga cgcacaggat ttagaagaat catatgaaat tgccagagag cgtggttttg
     1561 aagcagaaaa taagcggcga ataatgattg gtacctacgt gctttcaagt ggctactacg
     1621 atgcatatta caaacgtgct caaactgttc gcaccaaact cattaatgaa tttgcagaag
     1681 tatttacatc tgtagatttt ttggttggcc caacagcacc ttttaccgct tttaagcttg
     1741 gccaaaatgt aggtgaccca ttgcagatgt atttagcaga cattatgact gtggcagcca
     1801 accttgttgg tattccggcg attagtatcc cggctggaga ggtgaataaa ttaccaattg
     1861 gtattcagtt tatggcacca caactgcacg atcggcaact gctcggaatt gcccaggcaa
     1921 cggagaaatt actatgagca ttggtgtaat tattggcatt attgtcgtcg ctgtagtgat
     1981 actcgtgctt ttaaacctgg tggcaaaacc cagcacaaag gggatagata aagcgcattt
     2041 tattaatgaa tggaatgata ttgttgcttt aagtaaagat gctaaatctc ggccattaag
     2101 tattgtgcat gccgacaagc tcctagatga agctttaaaa ggcctg
//
LOCUS       DAOPLS010000131         2098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_61803_length_2098_cov_2.591777, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000131 DAOPLS010000000
VERSION     DAOPLS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61803_length_2098_cov_2.591777"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..769
                     /locus_tag="PKV52_03180"
     CDS             98..769
                     /locus_tag="PKV52_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99248.1"
                     /translation="MTKKVSVTSILMALLLTLSVIAMPIVVKAESEQNAAESISELRN
                     KKAEELKEKYQKQIEEARERAKQQVEAAKEKQEAKTVEVRVKNCESRANELQNKFKST
                     ITAAQRHQNTFNDFNKKIDNFVSMRSLELTNGASLKMTVVEAGAASQQAVDALATFST
                     EPIDCNAIDDVTQNVIAYKEALDTAKDALKAYRTATKNYLVAVKASALAEETSTDTQA
                     TEGAN"
     gene            772..1032
                     /locus_tag="PKV52_03185"
     CDS             772..1032
                     /locus_tag="PKV52_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99249.1"
                     /translation="MKQQGSLHIIVMIAVVLIGVIGFAGWRVYNTQKTNDVTTSTTQQ
                     PPVDTSVNSAKDVADLESQVDATNIDNDLDGSSLDNDLSNLQ"
     gene            1094..1543
                     /locus_tag="PKV52_03190"
     CDS             1094..1543
                     /locus_tag="PKV52_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013083925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HPD99250.1"
                     /translation="MIKKIVNNYNELATYFVWGVSWVSVFGSLYFSEIKGFVPCDLCW
                     WQRLLIYPLAIISTVAIIRKDYKNFMYYVLPFGVLGFIVSLYHSLLQWGVIEKNILDC
                     TATGVSCSKPEIEIFGFITIPFLSFLAFTALTIVSGISIYIQHKKVN"
     gene            complement(1559..>2098)
                     /locus_tag="PKV52_03195"
     CDS             complement(1559..>2098)
                     /locus_tag="PKV52_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD99251.1"
                     /translation="SLLKNQKELMKMLNEINPLGETLNKLYLKRMGDMTSAMVKGQFA
                     IAVAQGLSGTLSLWIIGFDYLAFWFFLLTFLSIIPLGGGIILIPLGIVLILTGNVWQG
                     VFLLAWHFIITTNIDNIMRPKFVPKSARMDPALTILSVFAGIGIFGFLGIVIGPVIMI
                     VILSTIQIYLEYKRKYMKA"
BASE COUNT          758 a          355 c          383 g          602 t
ORIGIN      
        1 agtacctatg gacactttgg taagcaaaac ttaccatggg aaaaactaac taatttggta
       61 cactaaaagc ataaccttaa ataaggaacg tgtgaatatg acaaaaaaag tatcagtaac
      121 atcaatactt atggctttat tgcttacgct atcagtaata gcgatgccta ttgtcgtcaa
      181 ggcagaatcc gagcagaacg ctgcggaatc aattagtgaa ttgagaaata aaaaagccga
      241 agaattaaag gaaaaatatc aaaaacagat agaagaggct agagaacggg caaaacaaca
      301 agtagaagca gccaaagaga agcaagaagc aaaaactgta gaagtaagag ttaaaaattg
      361 tgagtctaga gctaatgaac tacagaataa atttaaatct actattactg cagcccaaag
      421 acatcaaaat acttttaacg attttaacaa aaaaattgac aattttgtgt caatgagaag
      481 tttagaatta acgaatggtg catcacttaa aatgactgtt gtagaagccg gtgcagcctc
      541 acagcaagcc gtagacgcat tagctacttt ctctactgag cctatagatt gtaacgctat
      601 agatgatgtc acccagaacg tcatagctta caaagaagct ttagatacag ctaaagatgc
      661 actgaaagca tatagaactg ctacaaaaaa ctatcttgtg gccgttaaag caagcgcctt
      721 agccgaagaa actagtacag acacacaagc aacagaaggg gctaactaat tatgaaacaa
      781 caaggatctt tacatattat agtaatgatt gccgtagttc ttataggcgt aataggtttt
      841 gctggttggc gagtctacaa tacacaaaaa accaatgacg taactaccag caccacccaa
      901 caaccacccg tagatacttc agtaaatagc gcgaaagatg tggcagactt agagagccag
      961 gtagatgcta ccaatataga caatgactta gatggctcat ctctggacaa cgatttaagc
     1021 aatttacaat agattactag cataatctta gtcgtatagt gattatggtc ttttcgtata
     1081 taataagagc tatatgatca aaaaaatagt aaacaattat aatgaactag caacatattt
     1141 tgtgtggggc gtttcttggg ttagtgtatt tggcagtttg tattttagtg aaattaaggg
     1201 gtttgttccg tgtgatctat gttggtggca acggttgtta atttaccctc tggcaataat
     1261 atcaacagtg gcaattatta gaaaagatta taaaaacttt atgtattacg tactaccttt
     1321 tggtgtcctg ggttttattg ttagtcttta tcattcatta ctacaatggg gtgttataga
     1381 aaagaatata ctagattgca ccgccacagg cgtttcttgc tctaagccag aaattgaaat
     1441 atttggcttt attacaatac catttttatc gtttttggca tttacagcat taacaatagt
     1501 ttcgggtatt agtatttata ttcagcataa aaaagttaac tgagcaaaat aatataactt
     1561 aagctttcat atacttgcgc ttatattcta gatatatttg aattgttgat aagattacaa
     1621 tcataattac tggaccaata acaataccaa gaaacccaaa gatacctatt cctgcaaaaa
     1681 cagataatat tgtaagagcc gggtccattc gtgcactttt agggacaaat ttaggtctca
     1741 ttatattatc gatatttgtc gtaatgataa agtgccatgc taagagaaat accccttgcc
     1801 aaacattacc ggttagtatt aaaacaatac ctaaaggtat taatataatg cccccgccta
     1861 aaggaatgat agaaaggaac gttaataaaa agaaccaaaa cgccaggtag tcaaacccaa
     1921 ttatccaaag agaaagtgtt ccagacaaac cttgtgctac agctatggca aactgacctt
     1981 tcaccattgc agaggtcatg tctcccattc tttttaggta taatttattg agtgtttcac
     2041 ctagagggtt aatttcattg agcatcttca ttaattcctt ttggtttttt aataaaga
//
LOCUS       DAOPLS010000132         2096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_61874_length_2096_cov_4.488486, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000132 DAOPLS010000000
VERSION     DAOPLS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61874_length_2096_cov_4.488486"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..446)
                     /locus_tag="PKV52_03200"
     CDS             complement(132..446)
                     /locus_tag="PKV52_03200"
                     /inference="COORDINATES: protein motif:HMM:NF040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HPD99252.1"
                     /translation="MKFPWKNKEEPPKPRPMTDAEYLKLGKAVEQVYVSGYASKKQLF
                     KMSLYKGIGYGLGIFIGGTIIVALLAYTISQFETVPFLKDVLEPFQQTIQQVNGGPIA
                     PQ"
     gene            600..>2096
                     /locus_tag="PKV52_03205"
     CDS             600..>2096
                     /locus_tag="PKV52_03205"
                     /inference="COORDINATES: protein motif:HMM:NF021588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HPD99253.1"
                     /translation="MSRQSRSISSDVGLLAAAAVMPGTLQQSMIERDAVDQGLATGLT
                     MALAFSIAILMQDGIEAATNQFIDEDSRKDSFQAGILASGVAVGIGLIAQRLLHQTDS
                     EQMPRSIARTLSYWMTVAGAAGVTLRGLGAILGDDIENGGGKKQLASTLFIFPLGVLL
                     ALLFDFVKYRQIVSIKQNPVVSSPAKALTAGIAIIAGLTVVSRSEKYIAHHVQKYVDS
                     HAKPLAKGWLPIGHVASVATLAGIGWYGISKLYRKIESSQNTVEKIFSRNPDEIYVSG
                     STSSHVSWQTLSLQGRRHIGTRISKQQLNEDLHIKNALEPIRIYVGYDSAPSDEARTA
                     LALAEIDRTKALDRKYVVLIAPTGTGYVNYVMSDTVEYLAKGDCAMITMQYSKRPSPL
                     SLDRVDDGHIQFRILVNALSKRLRNMPKDKRPQILMFGESLGAWVSQDAFLYSGTDGL
                     IATGIDRALWIGTPELSRWQDIVNNGSTLNVDHELVAKFDNIADYRQLP"
BASE COUNT          609 a          413 c          471 g          603 t
ORIGIN      
        1 gtgcacacta cacagtgctc atattagcaa ttacaatgtt cattcatgta ccagaaacct
       61 ttgcaggact tgctggaata gccataattt ttagttcatt cgtagcatcc aagcgcgcag
      121 gtgattaaag actactgcgg tgctatagga ccaccgttaa cttgttgaat ggtttgttgg
      181 aaaggttcta atacgtcttt taaaaatggt acggtttcaa actgactgat ggtatacgcc
      241 agcagggcta cgataattgt accaccaata aatatgccta acccataccc tataccctta
      301 tataagctca ttttaaagag ttgtttttta gaggcatacc cagatacgta gacttgctct
      361 acggctttac caagttttaa atattctgca tctgtcattg gtctcggttt gggtggttct
      421 tctttattct tccatggaaa tttcatagtt ttatgataac agctttttgt aggctgtgct
      481 gaattactgg tatgtatgct gagagtcatt actttgtttg tttgatacat ttactattta
      541 tggtataaat atacataaga ccacttattg tacttggtat ttaataggac attgccacca
      601 tgagtagaca aagtagaagc atttcttcag acgttgggtt gttagctgca gctgcagtta
      661 tgcctggtac actgcagcaa tccatgatcg aacgcgacgc ggttgatcaa ggactggcta
      721 ctggactaac aatggcactc gcctttagca tagctatcct gatgcaagac ggtatagagg
      781 cagctacgaa tcaatttata gatgaggatt cgcgtaaaga cagctttcaa gcgggaatat
      841 tggcaagtgg cgtcgctgtt ggcattggct tgattgctca aagactatta caccagacag
      901 attcagaaca aatgccacgg agtatcgcca gaacccttag ttactggatg actgttgcag
      961 gagcggcagg cgttacactg cgtggcctgg gtgcaatatt aggtgatgat atagaaaacg
     1021 gcggcggtaa aaaacaactt gctagtacgc tatttatttt tccgcttggt gttttactcg
     1081 ctttgttgtt tgatttcgtg aaatatcggc aaattgtatc tatcaaacaa aaccctgttg
     1141 tttcatcacc agcaaaggca ttgacggcag gtatcgcaat aattgcagga ctcacggttg
     1201 tttcgagatc agaaaaatat attgctcacc acgttcaaaa gtatgttgat tctcacgcca
     1261 agcctctcgc taagggttgg ttaccgattg ggcatgtcgc tagtgtggct acacttgctg
     1321 gcattgggtg gtatggcata tcaaaactat atcgcaaaat tgaatcatca caaaatactg
     1381 tagaaaaaat attttctcgt aatcccgatg aaatctatgt gagtggaagt acgtctagtc
     1441 atgtatcttg gcaaaccttg tctttacaag gaaggcgtca tatcggtaca cgtatatcta
     1501 agcaacagct caatgaagat ttgcatatta aaaatgctct agagccgata agaatttatg
     1561 ttgggtacga tagcgcacca agcgacgaag ctcgcacagc attagcacta gcagaaattg
     1621 atcgcaccaa agcactcgat cgtaaatacg ttgtactcat agccccaaca ggtacaggct
     1681 atgttaacta tgttatgagc gataccgtgg aatacttagc caaaggtgac tgcgccatga
     1741 taacgatgca gtactccaaa cgaccgtctc cgttgtcgct agacagggta gatgatggac
     1801 atattcagtt tagaatactt gtcaatgcgc ttagcaagcg tttgcgtaac atgccaaaag
     1861 ataagcgacc acaaatcctt atgtttggcg aaagcttggg tgcctgggta agccaagatg
     1921 ccttcttgta cagcggaaca gacgggctca ttgctacggg aatagacagg gcactttgga
     1981 ttggcacgcc agaactcagt agatggcaag acatagtaaa taatggcagt acattgaatg
     2041 tggatcatga gttagtagca aagtttgata atatagcaga ttatagacaa ctacca
//
LOCUS       DAOPLS010000133         2068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_63080_length_2068_cov_5.154993, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000133 DAOPLS010000000
VERSION     DAOPLS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63080_length_2068_cov_5.154993"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="PKV52_03210"
     CDS             complement(<1..1816)
                     /locus_tag="PKV52_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HPD99254.1"
                     /translation="MFRKLISNLPFQPSLLSDVAFYAHRLKQEHAIRRIGFILILVGF
                     GIQIFAVAFPPEASLATNTSDIVYGATTKKDVLRAYRNNQDTQGRKDIRAIFDHYGIG
                     EAQIENATKTTIKDNNSNYINTSRSTTKYADTFVPIQGAIDGGIYEFPLKYWRKNEFP
                     NGYPALTGISTYGFRFWILLKGCGNIVYEKGAKKPNFEITKQLTSKSTINEGEKSTYD
                     IRFRNTGAIASNQTKITDSLPAGLEYVSYKSTADVRFHKKGQTLTWEIANKNSSLSPG
                     NRWHQITVTTKSTKATTKKLCNAVVINAGNAKRAEAKDTTCITVVKPTCPGTGLPIPA
                     GGVGACLVQCPDGSQIPYNQSCSVPQLSCSNLQVVAASAWNTRELQTTVASQPGAAVR
                     QVDFYINGQLAGSSQNATSQGVYSYTGSFPDAGTYIIKSEVIAANGQVQPSQNCMYEE
                     TINKPDVNGPILVTDKRVRNETQNIDNANNTTANPGDQLTYSLTITNQGASPAVDLAL
                     SGEYAEDIRDILEYADIVTLNDGVLNKETSKITWAATTIAPGQTITKTFAVKVKDPLP
                     ATPISLSNPLSYDYEMHNKFGRTVVVKLNKPGSKAIEQT"
BASE COUNT          531 a          402 c          452 g          683 t
ORIGIN      
        1 cagtttgttc aatagctttt gaaccaggtt tattcagctt aacaacaact gttcggccaa
       61 acttattgtg catttcatag tcatacgata atgggttaga caatgatatt ggtgtagcag
      121 gcaaaggatc tttgacctta acggcgaatg ttttagttat ggtttgcccg ggcgcaattg
      181 tagtagctgc ccatgtaatt ttgctggttt ctttattaag cacaccgtcg ttaagtgtca
      241 caatatcggc atattctaaa atatcacgta tatcttcagc gtattcacca gagagcgcta
      301 ggtctacggc tgggctggca ccttggttgg tgatagtaag actatacgtt agctgatcac
      361 cagggttggc ggttgtgttg ttcgcgttat caatattttg tgtctcattc cgaacacgtt
      421 tatctgttac caagattggt ccattaacgt ctggtttatt aattgtttct tcgtacatac
      481 agttttggct gggctgaacc tgcccattag cagcaatgac ctcacttttg attatgtacg
      541 tgcctgcatc agggaagctg cccgtatacg aatacactcc ttggcttgtt gcattttgtg
      601 aagagccagc taattgccca tttatgtaaa agtcaacctg tcgcacagca gcgcccggct
      661 ggctggcgac cgtagtttgc agttctctgg tgttccaggc tgatgcagcg actacttgta
      721 aattactaca actaagttgg ggtacactac aactttgatt atacggaatc tgcgagccgt
      781 ctgggcattg aactaggcag gctccaacac ctcccgcggg aataggtaac cctgtaccag
      841 ggcacgtagg cttaacaacc gtaatgcatg tagtatcttt tgcttcagca cgtttggcgt
      901 tcccggcatt aattaccact gcattacaca gcttcttagt agtggctttg gtagattttg
      961 ttgttaccgt tatttggtgc catctatttc ctgggcttaa actactgttt ttattagcta
     1021 tttcccaagt gagggtttga ccttttttat gaaagcgtac atcggcagta cttttgtacg
     1081 aaacgtactc tagcccagca ggtaagctat cggtaatttt tgtctggtta ctcgcaatag
     1141 caccagtatt acggaatcta atgtcgtatg tactcttttc tccctcattg atggtagatt
     1201 tagaagttag ttgtttggtt atttcaaagt tcggtttttt agcacctttt tcgtacacaa
     1261 tatttccgca acctttaagt aatatccaaa acctaaatcc gtacgtagat attcctgtta
     1321 gagcagggta accgtttggg aattcgtttt tgcgccaata tttaagggga aattcataaa
     1381 tcccaccatc tatggctcct tgaattggca caaaggtatc tgcatatttg gtagtgcttc
     1441 tagaagtgtt tatgtagttt gagttgttat ctttaatagt agttttggtt gcgttttcaa
     1501 tttgagcttc gccgataccg taatgatcaa aaattgctct tatgtctttt cggccttgcg
     1561 tgtcttggtt attacgatac gcacgtaata catctttttt agtagttgct ccgtaaacta
     1621 tgtctgatgt gttggtggct aacgaggctt ctggtggaaa agcgacagca aaaatctgta
     1681 tgccaaagcc aaccagtatt aatataaaac caatacgtcg tatagcgtgc tcctgcttga
     1741 gcctatgcgc ataaaaggct acatctgata gcaaagaagg ttggaatggt aggtttgata
     1801 ttaattttcg aaacattttt gtatatatac taatgaacaa ttattgtacc cttttatata
     1861 accagttatt acactaaaag gcaatattct tatggttgaa acattgccct gaaaaatata
     1921 taattgagtg tgatactaaa cagagagagt aagtagtagg gtatgagtaa acaaacagct
     1981 gcggccgcag cgtcttacga tgccggccag attcaagtgt tagaaggctt agagcctgtt
     2041 cgtaaaagac cgggtatgta tattggtg
//
LOCUS       DAOPLS010000134         2067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_63119_length_2067_cov_2.752982, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000134 DAOPLS010000000
VERSION     DAOPLS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63119_length_2067_cov_2.752982"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKV52_03215"
     CDS             complement(<1..756)
                     /locus_tag="PKV52_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99255.1"
                     /translation="MQSQRLPTEKVVDNSYINFARHSSMQSIESPPIDIWEEEIRNGF
                     APVDPITGIPLHIAPSEKPSWVRHRNDFDDNHHIFHWNSHALLQGQGGRVVRHSRLLR
                     GPRWAHQSYHEAFSNGISYLPDTDEEKFFIGIMACADIVPQYAVDVSRRTPSIVRLGE
                     KALQTMLSSRVFPEQDINKVNGKDNYFARRGYFFMQQAMDMGFAKVDTELLEQFLEER
                     DQETKRHFGMMVISAYLDKATAPIIPAFKLSRNS"
     gene            1066..>2067
                     /locus_tag="PKV52_03220"
     CDS             1066..>2067
                     /locus_tag="PKV52_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD99256.1"
                     /translation="MMPKLYEAIEALVAQGVPSQYIPNALIQAGWPPNLVNQAVDAWM
                     VTHGRKLQRTDFKNWVKKYHRMALPAVGVVVFINLIADGIALLKPWPLKILADSAFGE
                     IAAPGPLEPYTHTPTLILILSLISIALFILGALFGYVQDFLLLKIGFWLNRGIKAESL
                     NHILHLPLFHQERLAKGDYVYRQNIVTNSLSDLVLASTSSIIGSILMIIAILVIMLKF
                     SVTLTLFAVILIPFLFLTMKLVGPKLGKYNQALNEIASDTAAKVTESVDNAETVQAFT
                     LERKQLANINKLWEVGYLFTRKSMVWGKVLEDGNGLLIIIATSAVMFFGGSAALRGDI
                     "
BASE COUNT          573 a          425 c          408 g          661 t
ORIGIN      
        1 agaatttcta gaaagtttaa atgcaggaat aattggagca gttgccttgt ctaagtatgc
       61 gctaataacc atcatgccaa agtgtctttt ggtttcttga tctctttctt ctaaaaattg
      121 ctctagtaat tctgtatcta cttttgcaaa acccatgtcc attgcttgct gcataaaaaa
      181 gtatccacga cgcgcaaagt aattatcttt accattaact ttgttaatat cttgctctgg
      241 gaatactcgt gagcttagca tagtctgtaa tgctttttcg cctaacctaa ctatagaagg
      301 tgtgcgtctg ctaacatcta ctgcatattg cggaactata tctgcacacg ccataattcc
      361 aataaagaat ttctcttcgt ctgtatctgg taaatatgaa atcccattac tgaatgcttc
      421 gtggtagctt tggtgtgccc acctgggccc ccgtagtaac cgcgaatgcc tgacaaccct
      481 accaccctgt ccttgtaata aagcgtgaga attccagtga aatatgtgat gattatcgtc
      541 aaaatcattg cgatgtcgta cccaggatgg tttctctgat ggcgcaatat gtaatggaat
      601 acccgtaatg gggtctacag gagcaaaccc gttgcgtatt tcttcttccc atatatcgat
      661 tggtggtgat tctatagatt gcatagaact atgtcttgcg aagtttatgt aggaattatc
      721 cactactttt tccgtaggta atctttgaga ctgcatatta tacactcctc ttttttattt
      781 gtactcagct atcaaaaaaa cgcctttgca gtttttgcat tggcgctttt tgaagtgcga
      841 gcctgtacgt attgtgctta gtagccagca agtaaaggtg ttaagaatca tcaccatatg
      901 atgctgcata cgctcacgct cctttttttg atatttgcgt acctattttc atcatagtac
      961 cgccgttgta aaagtacaag tgttttatat aaatcattcc ttattgtgga ataaacagct
     1021 tgtgctttgc tataattgtg tttgaaagca ggtgtaaaca cagatatgat gcctaaattg
     1081 tacgaggcaa tcgaagctct cgtagcacaa ggggtgccca gccaatatat tccgaatgcg
     1141 cttattcaag ctggatggcc accaaacctt gtaaaccaag ccgtcgatgc ttggatggtt
     1201 acgcatggca gaaagttgca acgcacagat tttaaaaatt gggtgaagaa atatcaccgt
     1261 atggcactac cagctgtagg agtagttgtg tttatcaacc ttattgccga tggtattgcc
     1321 ctacttaagc catggcctct aaaaatattg gcagattctg cctttggtga aatagcagca
     1381 ccaggcccac tagagccata cacacataca ccaacactta ttttaatact atcgctcata
     1441 agcattgccc tgtttatctt gggcgcctta tttggctacg tacaagattt tttgttactt
     1501 aaaattggct tttggctgaa ccgcggtatt aaagcagaat cattaaacca tatattacat
     1561 ttacccctgt ttcatcaaga acgtctagct aagggagatt acgtttatcg ccagaatatt
     1621 gtgacgaata gtttgtctga cttagtgctc gcatctactt cttcaataat tggatcaatc
     1681 cttatgataa tcgctatttt ggtcattatg ctaaaattta gcgttacact taccttattt
     1741 gccgttatac ttattccgtt tttgttcttg actatgaagt tagttgggcc aaaactcggc
     1801 aaatataatc aagccctaaa cgaaattgca tctgataccg ctgctaaagt aactgaatcg
     1861 gtagacaatg cagaaactgt tcaagccttt accctagagc gaaaacaact ggcgaacatc
     1921 aacaagctgt gggaagttgg atatctgttc acacgcaaaa gcatggtgtg gggtaaagtg
     1981 ctagaagatg gcaatggcct gttgataatt atcgcaacat cagctgttat gttctttggc
     2041 ggctctgcag ccttacgggg tgatatt
//
LOCUS       DAOPLS010000135         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_63479_length_2059_cov_2.531437, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000135 DAOPLS010000000
VERSION     DAOPLS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63479_length_2059_cov_2.531437"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PKV52_03225"
     CDS             complement(<1..1056)
                     /locus_tag="PKV52_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99257.1"
                     /translation="MKKDIIYIDSDDEIAAVVDKVVASRSKVVALVLPKRFTMLHSSV
                     NMKILNKAANDNNKNLVLITNEKALMPLAAATGMYVAKGLQSKPAIPALAEPDTDLDD
                     AVEAPELDVEPTIDKTKPIGELAGDTDETITIDNTTPEDTAAKKPDTPKKSKKLAVPN
                     FEKFRLKLFLIIAGVIALLMLLYFAVFVWPKATVTLSVEQQEIPLNIDITASPTKEDN
                     VSEKVFKLDTKTIDKAETKSGEATGKKDLGEKATGEVTFSIPCSDVTSFPTTIPSGTG
                     VSTGGLTFITQKSVSLNNPPLPGSDCKFSGRAGVIAQNAGGEYNINKGRTFSVAGFSS
                     VSGSNKEGFSGGSSKVIT"
     gene            complement(1053..>2059)
                     /locus_tag="PKV52_03230"
     CDS             complement(1053..>2059)
                     /locus_tag="PKV52_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HPD99258.1"
                     /translation="RSISVISISLFGSTLNVRVTRKEPNKEIDITEIDRIFTLVQQRA
                     HDKAKKQLSIDTGGKDIALKLVNSALISIEIDGYSVTNPIGFQGKDVHVQLYTAFAPM
                     VHIGAIERVAEQLDLDLITVAAEPFAVARSVIGDDPNADISAVLIDVGGGTTDIAVVN
                     EGGVEGTVMYGIGGRSFTKSIERDFSVGFEQAEALKLGLSDGKTPDAKRKSVEKALTK
                     TLSVWSSGISLALEEFTRLDHLPNRILLCGGGSSLQLIRDELSQGNWYKDLPFTKKPQ
                     VQFISPNQVSGIHDSTNLVNDHTLITAMGLLRVGMDTVLQYEAELETIKGRFNKLLKI
                     "
BASE COUNT          554 a          447 c          393 g          665 t
ORIGIN      
        1 agtaattact ttggacgagc caccactgaa tccctcttta tttgagcctg atacagatga
       61 aaatccagca acactaaacg ttctaccttt gttaatatta tattcaccac ccgcattttg
      121 ggctataacc cctgctctac cactaaactt acagtcacta ccgggtaggg gtgggttatt
      181 aagactaacc gatttttggg taataaaggt taaaccacct gtgctgacgc ccgtaccact
      241 tggtatcgtt gtaggaaaac tcgttacgtc gctgcatggt atggaaaagg taacctcacc
      301 agtggctttt tcaccaagat cttttttgcc tgtcgcttca ccagattttg tttctgcttt
      361 atctatcgtc ttagtatcta atttaaagac cttttcagat acattgtctt cttttgttgg
      421 gctagctgtt atatcaatgt tcagtggtat ttcttgttgt tctacactta gtgtaaccgt
      481 cgctttgggc caaacaaaca cggcaaaata gagcagcatc aatagtgcta taacaccagc
      541 tatgattaaa aataatttaa gacgaaactt ctcaaaatta ggtaccgcaa gttttttact
      601 ctttttgggt gtgtctggtt tcttagccgc tgtatcttct ggcgtggtgt tgtctatagt
      661 aattgtttca tcggtatcac cagccaactc accaattggt tttgttttat ctatggttgg
      721 ttcgacatct agttcaggtg cctctacagc atcgtcgagg tcggtatctg gttcggctaa
      781 cgcgggaata gctggcttag actgtaaccc tttggcaaca tacataccgg tagctgctgc
      841 gagtggcatt aatgccttct cgttggtaat tagaacaagg tttttattat tatcattcgc
      901 agctttgtta agtatcttca tattaaccga gctatgcaac atggtaaaac gctttggcag
      961 tacgagcgca acaactttag accggctagc cactacttta tctacgacag ctgcgatttc
     1021 gtcatcacta tcaatgtaaa ttatgtcttt tttcatatct tgagtaattt attaaatctt
     1081 cctttgatag tttctaattc agcttcatat tgcaaaacag tatccatacc cacacgtaaa
     1141 agccccatgg ccgttataag ggtgtgatcg ttcactaaat ttgtgctgtc gtgtatgcca
     1201 gatacttggt tcggcgaaat aaactgaact tgtggttttt tggtaaatgg taagtcttta
     1261 taccagttgc cttggcttaa ttcatcacgt ataagctgca aactagaacc accaccgcat
     1321 agtaaaatac gattaggtag atgatctagc ctagtaaact cttctagcgc taaagatata
     1381 ccactagacc ataccgatag cgtcttggtg agggcttttt ctacagattt tcgtttggca
     1441 tctggagttt taccatcact aagcccgagt tttaaggctt ctgcttgttc aaaaccgaca
     1501 ctaaaatcac gctcaataga cttagtaaag ctacggccgc caataccata cataactgta
     1561 ccttctacac caccttcatt tacgacagca atgtctgtag tgccaccacc aacatctata
     1621 agtactgcac ttatatcagc gtttgggtca tcaccaatga cagagcgtgc aacggcaaac
     1681 ggttcagcgg caaccgttat taaatctagg tctaattgtt ctgccacacg ttcgattgca
     1741 ccaatgtgca ccataggtgc aaatgctgtg tacagctgga catgaacgtc tttaccctga
     1801 aaaccaattg gattagtaac gctataccca tctatttcta tagatataag cgcgctatta
     1861 acaagtttta gagcaatgtc cttgccacca gtgtcaatgc taagttgttt tttggcttta
     1921 tcgtgcgccc gctgttgcac gagggtaaat atacggtcaa tttctgttat atcaatttct
     1981 ttatttggtt ctttgcgtgt tactcgtaca ttaagggtag aaccaaataa agaaattgat
     2041 ataacagaaa ttgaccgta
//
LOCUS       DAOPLS010000136         2048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_63981_length_2048_cov_3.558956, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000136 DAOPLS010000000
VERSION     DAOPLS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63981_length_2048_cov_3.558956"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1704)
                     /locus_tag="PKV52_03235"
     CDS             complement(94..1704)
                     /locus_tag="PKV52_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99259.1"
                     /translation="MKNIEIPLVSERGKIYRLFEMVPGVLTWLTLSLPFVLSFIAPIL
                     AAYFIVAYLLMWFFKTFFMNVRMAQGYKKLQQSTSLDWYSHAQELDNPEPAFLRYPEG
                     ISPQWHYRNMIRHQARKGDRARLSELYQAVIIAVYNESPEVVEPTILSVASSNFPLDK
                     MVVILAVEERGGAEVRKNMQALVVKHKKLFKHLEMVVHPKDIPHEVIGKGGNITYAGR
                     RVDDYFKQQNIPARNVIVTTLDSDNRPHHQYFAAVSYAYLACPDPIHASFQPISIFTN
                     NIWDVPAPMRVIATGNSFWNIVLGLRPHMIRNFASHSQTLKTLQDTDFWSTRTIVEDG
                     HQFWRTYFAYNGHHEVHPMLVPIYQDAVLDVTYRKTLKAQFIQIRRWAWGASDIAYVL
                     NAGWRRKNSVPKVDLLFKTARLIEGHLSWATAPLLLLLGAFVPLYIAPEAGDSYIANQ
                     LPVIASYIQRIAMAGLFLSIYLSIRLLPPKPPRYKAHHWIPMLLQWVLLPLTTIIYSS
                     FAALYSQTRLMFGKYLGKFDVTTKAIKK"
     gene            complement(1704..>2048)
                     /locus_tag="PKV52_03240"
     CDS             complement(1704..>2048)
                     /locus_tag="PKV52_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99260.1"
                     /translation="TKKYFQDRPILFLNIAIVATALLNVIASVIRIDTSKSVAIVRYQ
                     AAELLTGYSRADTSELYSFAIASVFFAAVAIILSAKFFTQKRLMSVLLLSLTLIVLVM
                     LFVVSNAIYNLR"
BASE COUNT          606 a          432 c          421 g          589 t
ORIGIN      
        1 agcaagtgct gtaaaatact tgtcatttca ggctccgact aatttatcta gagatatacg
       61 caagatgctt cagtaaccta cgattttgcg tgattacttc ttaatagcct ttgtggtgac
      121 atcaaatttg cccaggtatt taccaaacat aagccttgtt tgagaataca gcgctgcaaa
      181 actactgtag ataatcgtgg tgagcggtag gagaacccat tgtaataaca ttggtatcca
      241 gtggtgagcc ttataacgtg gtggtttagg tggtagtaac ctaatgctaa ggtaaatact
      301 tagaaagagc cctgccatag ctatacgttg gatataacta gcgatgacag gcagttggtt
      361 cgcaatataa ctatcgcctg cctctggtgc aatatacagt ggtacaaacg ctcctaacag
      421 cagtagcagg ggcgcagttg cccacgaaag gtgcccttct attaatctgg cggttttaaa
      481 taacaagtct actttaggga cggaattttt tcttctccag ccagcgttaa gtacatacgc
      541 tatgtctgaa gcaccccagg cccatcggcg aatttgtata aactgtgctt tgagtgtttt
      601 gcgatacgta acatctagca cggcatcttg atatataggc accagcatag ggtgaacctc
      661 gtggtggccg ttataggcaa agtatgttcg ccagaattga tggccgtcct ctacaatcgt
      721 acgtgtactc caaaaatctg tgtcctgtag tgtctttagc gtctgcgaat gtgatgcaaa
      781 gttgcgaatc atgtgtggcc gtagccctaa aacaatattc caaaatgaat taccagttgc
      841 gataacacgc attggcgcag gcacatccca tatattgttc gtaaaaatag aaataggttg
      901 gaacgatgcg tgaatggggt ctggacacgc taaataggca tatgaaactg cggcaaaata
      961 ttgatgatgt ggcctattat cagaatctaa tgtcgtaacg atcacattgc gagccggaat
     1021 gttttgttgt ttaaagtaat catctactct gcgcccagca tacgtgatat tgccaccctt
     1081 accgataacc tcatgaggaa tatcttttgg atgcacgacc atttctaaat gtttgaacag
     1141 ttttttatgt tttacaacta gcgcttgcat atttttacgc acttctgctc caccacgctc
     1201 ttctactgca agtattacca ccattttatc tagaggaaaa ttgctactcg ccacacttaa
     1261 gattgttggt tctacgactt ctggcgattc gttataaaca gcgataatca ctgcttggta
     1321 tagttcactc agccgagcac gatcaccttt tcgagcctga tgacgtatca tattgcgata
     1381 gtgccactgc ggtgaaattc cctctggata ccgtaagaat gctggttcag gattatcgag
     1441 ctcttgggca tggctatacc aatctagaga cgtactctgc tgaagtttct tgtaaccctg
     1501 cgccatacga acattcataa aaaatgtttt aaagaaccac attaacaaat aagcgacgat
     1561 aaaatacgca gccagtatcg gtgcaataaa actcaataca aatggtaacg ataaagtcag
     1621 ccacgtcagc acaccaggta ccatttcaaa taaccgatat atcttaccgc gctcagagac
     1681 taaaggaatc tctatgtttt tcattagcgt aggttataaa ttgcgttact caccacaaac
     1741 aacataacaa gcacaataag cgttagtgat aacaacaaga cgctcatgag ccttttttga
     1801 gtaaagaatt tagcgcttag tataatagca acggctgcaa aaaatactga tgcaatcgca
     1861 aaactgtaca attccgaggt atctgctctg ctataccctg ttaataattc ggctgcttgg
     1921 taccttacaa tggcaacgga cttgctcgta tctatacgta tgaccgaggc aatgacgttt
     1981 aataacgcag ttgcaacaat agctatattt aaaaataaga ttggtctatc ttgaaagtat
     2041 ttttttgt
//
LOCUS       DAOPLS010000137         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_64064_length_2046_cov_3.462079, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000137 DAOPLS010000000
VERSION     DAOPLS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64064_length_2046_cov_3.462079"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKV52_03245"
     CDS             <1..185
                     /locus_tag="PKV52_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99261.1"
                     /translation="LSSNASLILILNLLAAASEFRMRIKDAFDDNGIEIPFPQRVVRT
                     VTESAPKKIVKSKSKK"
     gene            188..892
                     /locus_tag="PKV52_03250"
     CDS             188..892
                     /locus_tag="PKV52_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HPD99262.1"
                     /translation="MNELVGQFEFPLAYNLLATFLFALTGVILATKRQYDIIGVIILA
                     TVAGAGGGLIRDGIFLQQGPPLFLQDNRFLVAILLAVVVGTVFYHLVKKWDGVFFVAD
                     ALGLGIYGVIGAQMSINAGLGFISAVLVGLISATGGGLLRDILTKKEPVIFFPGQYYA
                     AAALAGVTLFLFLATVLKINAQIAAWVAIGLVFFFRLAAMKFDLSTKPLAAFADVNRT
                     KFLTFYDFVKPKRKSK"
     gene            complement(961..>2046)
                     /locus_tag="PKV52_03255"
     CDS             complement(961..>2046)
                     /locus_tag="PKV52_03255"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HPD99263.1"
                     /translation="TKFLYQKTVKPLLFTINPEKVHDTTLLAASTSGKIAPLRWLVKK
                     SWSYRDSSLTQTVDGIYYENPIGLAAGWDKDAKAISLMEPIGFGFNEVGSITQHPYAG
                     NEGTRLWRLPKSSSIAVYYGLKNQGVDTIAPRIAKTNSTIPVGTNIARTNSPNCDDAA
                     SIEDYAYSFKKLSHIGDYFTINVSCPNTFGGQPFHDKKRLDLLLSRLDTIKTKKPVYI
                     KLSPDITFKERQAIAKLAFTHNVQGFICGNLTKNRTLRSIYDENIPDVGGLSGKVVEE
                     LSNRLISDMYTLTNGQKTIIGLGGVFSGKDAYKKITLGASLVQLITGLIYQGPQCVGD
                     INKELACTLKNNGYNNVQEAVGTAHNH"
BASE COUNT          528 a          396 c          447 g          675 t
ORIGIN      
        1 cattgtcatc aaacgcatct ttaattctca ttctaaattt gctggctgca gccagcgaat
       61 ttagaatgag aattaaagat gcgtttgatg acaatggcat agaaataccc ttcccacaac
      121 gagttgtacg caccgtaaca gaatcggcac ccaaaaagat agtaaaaagc aaatctaaaa
      181 agtaataatg aacgagctag ttggccagtt tgaatttccg cttgcttata atttattggc
      241 aacatttttg ttcgctttaa ctggcgttat actggcaaca aagcggcagt acgatattat
      301 tggtgtgatt attcttgcga ctgtcgccgg ggctggtggt ggcttaatac gcgatggtat
      361 ttttttgcag caaggtcccc cactgttttt gcaagataac cgttttttgg ttgccatttt
      421 actggcagta gttgttggta cggtgtttta tcatttggtg aaaaagtggg atggagtgtt
      481 ttttgtagca gacgcacttg ggcttggtat ctatggtgtt ataggagcgc agatgagcat
      541 taatgctggg cttgggttta tatcggcggt acttgtaggg ctaatatctg caacaggcgg
      601 tggtttactg cgtgatattc ttaccaaaaa agaacccgtt atcttcttcc cgggtcagta
      661 ctatgcagcg gctgctttgg caggtgtcac actatttttg ttcttagcca cagtattaaa
      721 aatcaatgcc caaattgctg catgggtggc tatagggctt gtgttctttt ttagattagc
      781 agccatgaag tttgatttaa gcaccaagcc actcgcagcc tttgcagatg tcaatagaac
      841 taagtttctt acattctatg actttgtaaa accaaagcgt aaatcaaaat aatcagtaaa
      901 atagtacagc tacacggcct atgttacgtc gcattaaatt tgtgtagtac gtgtcgtttg
      961 ctagtgattg tgagcggtgc caactgcttc ttgtacgttg ttgtagccat tgttttttaa
     1021 agtacaggca agttctttgt taatatcacc tacgcattgt ggtccttggt aaataagccc
     1081 ggtgataagc tgaaccaaac tcgctccaag cgtaattttt ttgtaggcat ctttgccgct
     1141 aaatactcca ccaagaccaa taattgtttt ttggccgttg gtaagcgtgt acatatcgct
     1201 gatgagtcta ttagataatt cctctacaac cttgccactt agacccccca catctggtat
     1261 attttcatca taaatactgc gtaaggttct attttttgtc agattgccac atataaatcc
     1321 ttgcacattg tgcgtaaaag ctaatttggc gatggcttgt ctttctttga acgtaatatc
     1381 cggtgatagc ttaatgtaaa caggtttttt cgtcttgatt gtatctaacc tagataatag
     1441 taggtcgagt cgttttttat catggaatgg ttggccacca aatgtgttgg ggcaactaac
     1501 attaatcgta aaatagtcgc cgatgtgcga gagttttttg aatgaatatg cataatcttc
     1561 tattgacgca gcatcgtcgc agttaggtga atttgtgcgg gctatatttg tacctaccgg
     1621 tattgtgctg ttcgtcttag cgatcctcgg tgctatcgta tctacacctt ggtttttaag
     1681 accataatat actgcaatgc tactggattt aggtaagcgc cagagccgtg taccttcgtt
     1741 accagcatag ggatgttgcg tgattgaccc tacttcgtta aacccaaaac caattggttc
     1801 cataagacta attgctttcg catctttatc ccaccctgcc gctagtccaa taggattttc
     1861 ataataaata ccatcaactg tttgtgtgag cgaagagtcg cggtagctcc aggacttttt
     1921 aacaagccac cgtagcggtg ctattttacc gctcgtgcta gcagctagca atgtggtgtc
     1981 atgcactttt tctgggttaa tggtaaaaag taacggttta acagtttttt ggtacaagaa
     2041 ttttgt
//
LOCUS       DAOPLS010000138         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_64523_length_2035_cov_4.959596, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000138 DAOPLS010000000
VERSION     DAOPLS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64523_length_2035_cov_4.959596"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKV52_03260"
     CDS             complement(<1..660)
                     /locus_tag="PKV52_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99264.1"
                     /translation="MIQINLLPSIKAEYVKAQRTKRTVITISIIAVAVSLGVVGLLAS
                     VAYGTQQLQLNNLKNDIAKSEQNIKDVKDLDKILTIQNQLFALTPLHESKPVMERLFT
                     YLQQTTPDSVVLEKYVINNEDFTWSVDGEAPSIELVNKYVDTLKFTEIAPIQENQEKQ
                     YAFSEVVLSSFSKSEKGYTFTIDFKFSDQLFSSANTDIKLLIPNITTTRSQTQLPSSA
                     IF"
     gene            complement(657..1697)
                     /gene="pilM"
                     /locus_tag="PKV52_03265"
     CDS             complement(657..1697)
                     /gene="pilM"
                     /locus_tag="PKV52_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPD99265.1"
                     /translation="MSVLSGVSDFFGLDIGTSAIRLVQLRGSGPVKALVKYALTPIDT
                     KLVLSDAKADQQKVASIIKELVAQARVNTKNVTVGLPSQRVFTTVVDIERLSPSELAK
                     NIKFQADTLIPTPLDESKIDWALLGDSPVDANKVEVLISSVANTYVEQRLDTLEAIGL
                     NVIAFEPDTIALARAMTSSDDTAPQVVVDIGTNYSDLVIVAGGTPKLNRSIPTGSDAI
                     IKAAMQGLGIDDKQAEQFVYKFGLSKDKLEGQIYAAIIGTVETLVSEIEKSIKFYQTR
                     YPNAPITKIVVTGGASTLPEFPLFIANKFGINVEIGNAWRNVTFPADKQNELLSVSNH
                     FGVAAGLAERIE"
     gene            complement(1750..>2035)
                     /locus_tag="PKV52_03270"
     CDS             complement(1750..>2035)
                     /locus_tag="PKV52_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99266.1"
                     /translation="PLNINGAVSAPTIDFRRGGGSRYLNTSPGDEQQNCNVSKGIQNC
                     ADRGNMPNNTGKSAEIINYPAYLYFAKPYLKNQSTPGDTVDAMFVVPPKL"
BASE COUNT          531 a          401 c          415 g          688 t
ORIGIN      
        1 aaatattgca ctcgatggca actgagtttg cgaccgagta gtcgtaatat taggtataag
       61 caattttata tctgtatttg cagaagaaaa tagttgatcg ctaaacttaa aatcaatcgt
      121 aaaggtataa cctttttctg atttgctaaa cgatgataat actacttcgc taaacgcata
      181 ttgcttttct tggttttctt gtattggtgc gatttctgtg aactttaagg tgtctacata
      241 tttattcaca agttctatgc ttggtgcttc gccatctaca gaccacgtaa aatcttcgtt
      301 attaatgacg tacttttcta gtaccacaga gtctggagtt gtttgctgca agtacgtaaa
      361 taatcgttcc ataactggct tagattcatg caatggggtg agtgcaaaaa gctggttttg
      421 aatggtaagt attttgtcta aatcttttac gtctttaata ttttgttcac ttttagctat
      481 gtcgtttttt agattattaa gctgaagttg ctgagtacca tacgccacgc tagctaacaa
      541 accaaccacg ccaagagaca cggccaccgc gataatagaa atagttataa cagtacgctt
      601 tgtgcgttgt gcttttacgt attctgcttt aatgcttggt agtaagttaa tctgtatcat
      661 tctattcgct ccgccaagcc tgctgccaca ccaaaatggt tgcttacaga aagtagttcg
      721 ttttgtttat ctgctgggaa agtgacatta cgccaagcgt tgcctatttc tacattgata
      781 ccaaacttat tggcgataaa cagcgggaac tccggtagtg tagatgcgcc accggtgaca
      841 actatctttg taattggtgc gtttgggtaa cgtgtttggt aaaacttaat tgacttttct
      901 atttctgata ctaatgtttc tactgtgcct ataattgcag cgtatatctg gccttctagc
      961 ttgtctttac ttaggccaaa tttataaaca aactgctcgg cttgtttatc gtctatgcca
     1021 agcccttgca tagcagcttt tataatagcg tccgaacctg tggggataga cctgttaagt
     1081 tttggcgtac cgccggcaac aatgacgagg tctgaataat ttgtaccaat gtctacgact
     1141 acttgtggag cggtatcatc actactggtc atggctcgcg ctagtgcaat ggtgtctggt
     1201 tcaaaggcaa taacatttaa cccaattgct tctagtgtgt ctagacgttg ctctacataa
     1261 gtgttggcaa cactagaaat aagtacttct actttgttgg catctactgg tgaatcaccc
     1321 aatagcgccc agtctatttt actttcgtct agtggcgtgg gtatgagtgt gtctgcctga
     1381 aatttaatat ttttggcaag ttcagatggg ctaaggcgtt ctatgtctac aacagtagta
     1441 aaaacacgtt gcgatggcaa accaaccgtc acatttttag tattcactcg tgcttgggcg
     1501 acgagctctt ttataatgct ggctactttt tgttgatctg cttttgcgtc tgatagaact
     1561 aattttgtat ctataggggt aagtgcgtat tttactagcg cttttacggg cccacttcct
     1621 cttaattgta ctaggcggat tgccgacgtt ccaatatcaa ggccaaaaaa gtctgagacg
     1681 ccacttagga cactcatagt gtttattcta aagcataacc gctacacaag caaacaaatt
     1741 atagttattt tacaactttg gtggtaccac aaacatggca tccacggtat cgccaggagt
     1801 gctttggttt ttaaggtatg gtttagcaaa atataaatat gctgggtagt taataatttc
     1861 tgctgatttg ccagtattgt ttggcatgtt acctctatct gcacagtttt gtatgccctt
     1921 actaacgttg cagttttgct gctcgtcacc tggtgaggta tttaagtacc tactaccacc
     1981 gcctcttcta aaatctatag taggtgcaga aaccgctcca tttatattta aaggc
//
LOCUS       DAOPLS010000139         2025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_65006_length_2025_cov_3.229949, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000139 DAOPLS010000000
VERSION     DAOPLS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65006_length_2025_cov_3.229949"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..>2025
                     /locus_tag="PKV52_03275"
     CDS             120..>2025
                     /locus_tag="PKV52_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99267.1"
                     /translation="MNLNCYSTSNETGIIYAYDIDNYADNNARGAQDNNSPGAPYNRK
                     TFDYDGSNNGRGKVLDGYVFKDLYFGLTRKDTGNRMKAIDTSTSASKWAKEVGWDKTD
                     ANRYKPLHDATQASNWESSDNFAWLKYTIKRGINYKLTINDLNAQNYLSLMVFPMNEQ
                     PPCNNPPKWTISGSASRQRTAFTGQTVSYSHSIENIGPDKNDESIDGDVTGDTGMADD
                     YTSYDIPTDSNVGTKRQTGVGTVPARSIVMNNVGNYCDTLSFTPTSWNSDATTSTSDC
                     VHIIDPDGDVPTTVSYEKGSESISINEADVKVNNGGSCPSTPIRIPFSWSIGGVSSPA
                     GTGFWYGGAYGCNPPTNVIPIPAAIIGQLNQSAPGLSGIKYCLSVNNEQQKCGEILVY
                     EVPFARFYGNDIYATAGDIRFNSTDNPSGTTKRGSVDQYAALAFGTVTIDTSAFRTIN
                     PVPPDGLDASGSKAISGKNANQVYTDVTGNLPKTCGEIESGGLNATEDGCYIIKKATS
                     LTPTNNSLPVPDWAKDNGGIPVLGWGDTFGSGVTTYTKKITAVNPTDKMLMIVGDVIN
                     LTSAANYTDPAKTGVLLIISEGPIVVDNNVKRIDAILVSKTGIYTCGYAGYGPAAKVG
                     QNQIHSTCRVP"
BASE COUNT          650 a          385 c          441 g          549 t
ORIGIN      
        1 aggtacaggt ggatgaagca aagcatatcg gttttgtagg cagtctcagc tatgattata
       61 cagtccccat agcgatagac tccacagaca gctctgtata caccgacata aatatcaata
      121 tgaatttaaa ttgttattcg acttctaatg aaacaggaat aatttacgca tatgacatag
      181 ataattatgc agataacaat gctaggggcg cacaagataa taacagccct ggagctcctt
      241 ataatcgaaa aacatttgat tatgacggat caaataatgg taggggtaaa gtacttgatg
      301 gttatgtttt taaagattta tactttgggc ttacaagaaa agacaccggc aatcgaatga
      361 aagctataga tacaagcaca tctgcaagca aatgggctaa agaggtaggt tgggataaaa
      421 ctgacgcaaa tagatacaaa cctttgcatg acgcaaccca agcaagtaat tgggagagtt
      481 ctgataattt tgcatggtta aagtatacaa ttaaacgtgg tataaattac aaattaacta
      541 ttaacgatct taatgctcaa aattatttat cactcatggt gttcccgatg aatgaacagc
      601 cgccatgtaa caacccccct aaatggacta tttctggtag tgctagcaga caacgcacag
      661 cttttaccgg gcaaacggtt agttattctc attctataga aaacattggc ccagataaaa
      721 acgatgaatc tattgacggc gatgttacgg gtgatacagg tatggcagat gattacacat
      781 catacgatat acctactgat tcgaacgtag gcaccaagag acaaacaggt gttggaacgg
      841 taccagcacg gtctattgta atgaataatg taggtaatta ttgtgatacg ttatcattca
      901 caccaacaag ctggaatagt gacgctacaa cgagcactag tgattgtgtg cacattattg
      961 accctgacgg tgatgtgcct actactgtta gctacgagaa gggcagcgaa agcatttcta
     1021 tcaacgaagc tgatgtaaaa gtaaataacg gcggaagttg cccgtcgacc ccgatacgga
     1081 taccatttag ttggagcatt ggtggagtgt catcacctgc gggtactggt ttttggtatg
     1141 gtggggctta tggctgcaat ccgccgacaa atgttattcc aattcctgca gcaattatcg
     1201 gtcagctcaa ccaatcagcc ccaggcctca gcggtattaa atattgccta agtgttaata
     1261 atgaacaaca aaaatgtggt gaaatcctcg tctatgaagt cccctttgct cgtttttacg
     1321 gtaatgacat atatgcgaca gcaggcgata tacgctttaa ttctacagat aaccctagtg
     1381 gtaccactaa aaggggtagt gtagaccaat atgcagcgtt agcgtttggt acagttacta
     1441 tagatacatc ggcgtttcgt accattaacc ctgtaccacc agatggtcta gatgcctctg
     1501 gctcaaaagc tatatcaggt aaaaatgcca accaagtgta tacagatgta actgggaatt
     1561 tgccaaaaac atgtggtgaa attgagagtg gcgggctcaa tgctactgaa gacggttgtt
     1621 atattataaa aaaagctact tctttaactc ccaccaataa ctcattgcct gttcctgact
     1681 gggctaaaga taatggtggc atacccgtat taggctgggg tgatactttt ggcagtggag
     1741 taactacata taccaaaaaa ataacggccg tcaatcctac agataagatg ttgatgattg
     1801 taggtgatgt catcaatctt actagtgcag caaattatac ggatcctgca aagactggtg
     1861 tgcttttaat tatttccgaa ggtcccattg tagtagataa caatgttaag cgcatagatg
     1921 caattttagt ttctaaaaca ggtatatata cctgtggata cgcaggttat ggcccagctg
     1981 caaaggtagg gcaaaatcag atacattcaa cctgtagggt gcctt
//
LOCUS       DAOPLS010000140         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_66014_length_2004_cov_4.684967, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000140 DAOPLS010000000
VERSION     DAOPLS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66014_length_2004_cov_4.684967"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..600)
                     /locus_tag="PKV52_03280"
     CDS             complement(109..600)
                     /locus_tag="PKV52_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99268.1"
                     /translation="MVTIAKIVFASVVLLVGTVAPASAMSAPLSRVSASAQPAYVSSA
                     GCPTRKWKIYDQYVDTVFHESVRVWVKLCVVRHKGNDRVTAVKTLVKSRKVRGIELHL
                     HSMRYLDGATTDYWSGGELRKGRWHVIYPHVECYARCQLAVVITINKNYYFDQTAGFT
                     VSR"
     gene            complement(669..1388)
                     /locus_tag="PKV52_03285"
     CDS             complement(669..1388)
                     /locus_tag="PKV52_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPD99269.1"
                     /translation="MSQAKQRLDQAVVSRGLALTRSQAENSIRLGYIQVDKKVIQKPG
                     YFVAPSAVITNIAKEIYVSRAGLKLASVAQLLKLDFNGKVVLDVGSSTGGFTDYALQH
                     GAKKVFAVDVGTQQLHPSLRGNTKISLHEQTDIRNFKPTEHIDVVVIDVSFISLRDVL
                     PHIATIISKKTLVVAMCKPQFEAGAGSKHHGVIKNDAMRRRILRDFETWVKQYFIVLH
                     KADSDIKGAKGNIERFYLLKI"
     gene            complement(1426..1785)
                     /locus_tag="PKV52_03290"
     CDS             complement(1426..1785)
                     /locus_tag="PKV52_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99270.1"
                     /translation="MSERNKRDFLGLPWDAKSFNITEKAESTLGKLRSFIVEQAGVVT
                     DTAKTATDTVVDKATATTSTVQERSKNAYTTVSDLGSSAVESVFGRMQAITADLKVRL
                     LGDDEPSLGDRPERGEK"
     gene            complement(1769..>2004)
                     /locus_tag="PKV52_03295"
     CDS             complement(1769..>2004)
                     /locus_tag="PKV52_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPD99271.1"
                     /translation="KVKAQSFDGRVISIELPKNVYLAVTYTEDVVKGDTTSSVLKDAT
                     LETGIIVKVPAFIKQGDILSIDTETGAYRERKK"
BASE COUNT          478 a          491 c          434 g          601 t
ORIGIN      
        1 agcgagtgtt gcaaaggtgt tgaactaatt acggcataaa aaatacccac tggtgtgggt
       61 tttgggtggg cgctgggctg caaagcccta gcgcccaccc gtggtgtctt agcgactgac
      121 ggtgaagcct gccgtctggt caaagtagta gtttttgttg atggttatga cgaccgccaa
      181 ctggcaccta gcgtagcact cgacgtgcgg atagatgaca tgccaccgac ccttgcgcag
      241 ttcaccgcca gaccagtagt ctgtggtcgc tccgtctaga tagcgcattg agtgcaaatg
      301 tagctcgatg ccacgcactt tgcgtgactt cacaagcgtt ttgaccgcgg tgacgcgatc
      361 gttacccttg tggcgaacaa cacacagctt gacccaaacc ctgacacttt cgtgaaagac
      421 tgtatctacg tattgatcgt agatcttcca cttgcgtgtt gggcaaccag cgctgctcac
      481 gtacgcaggt tgtgcgctgg cactcactct gcttagcggt gcagacattg cactcgcagg
      541 cgcaaccgtg cccacgagca aaacaacgct cgcaaacaca atcttggcga tggtaaccac
      601 tctacatcac tccctctatg atggacgtgt taagacttaa ctacttaaac atatttttat
      661 gcttttgtct atatctttag caggtaaaaa cgttcgatat tgccctttgc gccttttata
      721 tctgaatctg ctttatgtaa cacaataaaa tactgcttca cccacgtttc aaaatcacgc
      781 aaaatgcggc ggcgcatagc atcatttttt atcacaccgt ggtgtttact acctgccccg
      841 gcttcgaact gtggcttaca catggctacc accagtgttt ttttagaaat tatcgtagcg
      901 atatgcggta aaacgtcgcg caagctgata aacgaaacat caataacaac cacgtctatg
      961 tgttctgtgg gcttaaaatt tcgtatatca gtttgttcgt gaagactaat ttttgtgtta
     1021 ccgcgcaaac ttgggtgcag ctgttgcgta cctacatcta cagcgaatac tttttttgca
     1081 ccatgttgca atgcgtaatc tgtaaaacca ccggtactgc tgccgacatc cagtacaact
     1141 ttgccattaa aatcaagttt tagcagctgc gcgacactag ccagtttaag gcctgcccgg
     1201 ctcacgtata tctcttttgc aatattagta atcacagcgc taggcgctac aaaatacccg
     1261 ggcttttgga taactttttt atctacctga atatagccaa gtcgaatact attttctgcc
     1321 tgagaacgcg ttaaggcaag accgcggctg acgactgctt ggtctaagcg ttgcttggct
     1381 tgggacatgc gcgctccttg ctctttaaag gctgcctata gcaacctatt tctcaccacg
     1441 ttctggtctg tcgcctagtg atggttcgtc atcacctagt agacgtactt ttaaatctgc
     1501 tgttatggct tgcatgcggc caaagacaga ttctacagcg gagctcccaa gatcactgac
     1561 ggttgtatac gcattttttg aacgttcctg tactgtgctt gttgtggcag tggctttatc
     1621 tacgactgta tctgtcgcag ttttagcggt atctgttact acaccagctt gctctacaat
     1681 aaagctacgg agcttaccta gtgttgattc ggctttttct gttatgttaa atgactttgc
     1741 gtcccatggt aaccctaaaa aatcacgttt atttctttcg ctcacgatat gctcctgttt
     1801 cagtatctat tgataaaatg tcgccctgct taataaaagc aggtactttg actattatac
     1861 ctgtttctaa cgtagcgtct tttaaaacgc tgcttgttgt gtcgcctttt acaacgtctt
     1921 ctgtgtaggt aaccgccaaa tagacgtttt ttggtagttc tatgctaata acgcgcccat
     1981 caaatgactg cgcttttact ttgt
//
LOCUS       DAOPLS010000141         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_66465_length_1996_cov_4.300876, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000141 DAOPLS010000000
VERSION     DAOPLS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66465_length_1996_cov_4.300876"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..103
                     /locus_tag="PKV52_03300"
     tRNA            17..103
                     /locus_tag="PKV52_03300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51..53,aa:Leu,seq:taa)
     gene            178..741
                     /locus_tag="PKV52_03305"
     CDS             178..741
                     /locus_tag="PKV52_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPD99272.1"
                     /translation="MIVLWIVLGIVVLLAIYLIATYNGLVKLNVRVEEAWSDITVQLK
                     RRLDLIPNLVNTVQGYASHEKSVFTKVTEARANAMNAQSVAETAQADNMLSDALKSLF
                     AVAEAYPDLKANENFKQLQEELVDTEDKIQASRRFYNGSARDLNIKIKTFPNNIFANS
                     LGFKEREFFELDEAETAKAEKPVDVKF"
     gene            757..1638
                     /gene="htpX"
                     /locus_tag="PKV52_03310"
     CDS             757..1638
                     /gene="htpX"
                     /locus_tag="PKV52_03310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPD99273.1"
                     /translation="MYSEIAANKRKTIFLILGFFFLIAAVAAGAAWYFGDYFITIYAL
                     VFALGYIAWSYFGSDKAALAINNAQEIQKSDNPRLWRTVENLAITNGLPMPKVYIMDD
                     PALNAFATGRDPRHASVAVTTGLLNTMDDSELQGVMAHEMGHIQNYDIRVAIVVFGLV
                     AVVSIISDMILRGLFWGSFNNDNRNSSPIMYIVLIVALIIAPIVATIIQLSISRKREY
                     LADATGALTTRYPEGLASALEKIEHSSSAMQKQSTSTAHLFFANPLKGKNIAGLFSTH
                     PPIEDRIARLREMGTKA"
     gene            1635..>1996
                     /locus_tag="PKV52_03315"
     CDS             1635..>1996
                     /locus_tag="PKV52_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99274.1"
                     /translation="MKKKQKTHTKKVVSYKQLTKMTWLDISSFWRFLGGILVVYAVIN
                     FVFVASFSLLPSSESLQEDIIGTLGTSAGRVVDSFTLVGVSLINVSAPSNTMLQLLLL
                     AIVSMAMIWALRKLRGLQ"
BASE COUNT          602 a          379 c          425 g          590 t
ORIGIN      
        1 tgttgtatat ctacatgggc gagtggcgga attggtatac gcgacggact taaaatccgt
       61 tggcagaaat gccttgaggg ttcaagtccc tcctcgccca ccataaacag taaaatatta
      121 ctactagcaa caccgttata ttgcgtctat aattagtggt ataaaggagt tttaaatatg
      181 attgtactat ggatagttct tggtattgtt gtactgctag caatttacct tattgctaca
      241 tataacggtt tggtaaaact aaatgttcgg gtagaagaag cctggagcga cataaccgtc
      301 cagctgaaac gtcgtttaga cttgatacca aatttggtca acactgttca agggtatgcg
      361 tcacatgaaa aaagtgtgtt taccaaagtt accgaagctc gtgccaatgc aatgaacgct
      421 cagtcagttg cagagacagc acaggccgat aacatgctca gtgatgctct taagagttta
      481 ttcgctgttg cagaagcgta tccagactta aaagcgaatg aaaattttaa acagttgcaa
      541 gaagaacttg ttgacacaga agataagata caagcatctc gccgcttcta taacggttcg
      601 gcacgtgatc ttaatattaa aatcaagacg tttccaaaca atatttttgc caatagccta
      661 ggctttaaag agcgcgaatt ctttgagctt gatgaagcag agaccgccaa agccgaaaaa
      721 cctgtagacg taaaatttta actaaactcg acacatatgt atagcgaaat tgccgccaac
      781 aagcgcaaaa ctatttttct gatactcggc ttttttttct tgatagctgc tgtagctgca
      841 ggggctgctt ggtattttgg tgactatttt attactattt acgcactcgt atttgctcta
      901 gggtatattg catggagcta ttttggttca gataaagccg ccttagcaat aaataacgct
      961 caagaaatcc aaaaatcaga caacccaaga ttgtggcgta cagtagaaaa tttggcgatt
     1021 actaacggtt tacctatgcc caaggtctat attatggacg atcctgccct caatgcattt
     1081 gccactgggc gtgatcctcg gcacgcttct gtcgctgtta ctaccgggct attaaatact
     1141 atggacgata gtgagcttca aggagttatg gcgcatgaaa tggggcatat tcaaaactac
     1201 gatatacggg tcgcaatagt agtatttggc ttagttgcag tagtttctat tattagcgac
     1261 atgattttac gtggtttatt ttggggtagc tttaataacg ataacagaaa tagcagtcca
     1321 ataatgtata tcgtgctcat tgtagccctt ataattgccc ccatagtggc gacgattatc
     1381 cagctttcta tttctagaaa aagagaatat ttagctgatg cgactggggc gctcacgaca
     1441 cgttatccag aaggtcttgc cagtgcacta gaaaaaatcg agcacagcag ttctgctatg
     1501 caaaaacaaa gtacatctac agctcattta ttttttgcaa acccgctaaa aggtaaaaat
     1561 atagcgggtt tgtttagcac tcatccacca atagaagata gaatagcccg attgcgtgag
     1621 atgggcacaa aagcatgaaa aagaaacaga aaactcacac caaaaaagta gtttcataca
     1681 aacaactcac taaaatgaca tggttagata ttagctcttt ttggcgattt ttaggtggta
     1741 ttttagttgt ctatgcggtt attaattttg tgtttgtagc gagtttttcg ctgttaccaa
     1801 gcagtgaatc tttgcaagaa gatatcatag gtacacttgg cacaagtgct gggagagttg
     1861 tagactcttt tacgcttgtt ggtgtttcac tcattaatgt ttctgcccca agcaatacca
     1921 tgctgcaact actgttgctt gcaatcgtga gtatggctat gatttgggct ttaagaaagt
     1981 tacgtggctt acaaaa
//
LOCUS       DAOPLS010000142         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_66470_length_1996_cov_4.107676, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000142 DAOPLS010000000
VERSION     DAOPLS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66470_length_1996_cov_4.107676"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1958
                     /locus_tag="PKV52_03320"
     CDS             81..1958
                     /locus_tag="PKV52_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HPD99275.1"
                     /translation="MGLFKKKKQQLDPVALAEQQRLQEQQEVQAAFQKGVTALRDFIA
                     PSSIEFSSSYFQIGTRFARTYYVFGYPRQIHTGWISSFVNIDEVVDLSLFIYPVESQV
                     VLENLRKKVSQLEAGLQIDAEKGRVRDPGKQAAILDAEELRDKLQVGEEKFFRFGMYF
                     TVYGNSLDELDFVSHKIESMLGQQLVFSKPASAQQEQGLNSTIPQFVDQLQIRRNMNT
                     GAIGTSFPFTSADLSDDHGILYGINMHNSGLVIFDRFSLENGNSVVFAKSGAGKSFAV
                     KLEALRSLMLGVEIFIVDPENEYERMSEAVGGAYVKLSLNSPTRINPFDLPLVVDTEE
                     ADNALRSNLITLHGLLRLMMGGAQAQMAASQGSVVAPALNPTEEADLDAALLETYAKA
                     GITNDPLTHNSTPPTIHDLYDTLLHMGGTGPQLAQRLRKYTTGTFSGIFSQQSNVDIN
                     NPFVVFNIRDLEDELRPVAMYIVLNYIWNKTKSDQKRRMLVIDEAWQLMKYEDSASFM
                     FSLAKRARKYNLGITTISQDVEDFMSSRLGRAIVANASMQILLKQSVSAVDVLADVFK
                     LTSEEKKRLSQFPVGQGLFFAGQSHVHIQIAASPSETSLITTNPEQIKLLEDQGQIPM
                     R"
BASE COUNT          640 a          423 c          440 g          493 t
ORIGIN      
        1 ttgcagattt aactgcgccg gtggtaacta aagggcaagg caacgctgcc cagccaaacc
       61 tagataggga gatgcaataa atgggtcttt ttaagaaaaa aaagcagcag cttgaccctg
      121 tggcgcttgc cgaacaacaa cgtttgcaag aacaacaaga agttcaggcg gcattccaaa
      181 aaggtgttac tgcactccgt gactttattg caccaagtag tattgaattt agtagtagtt
      241 attttcagat tggtacccgt tttgcacgga cctactacgt ttttggctac cccaggcaaa
      301 tacacacagg ctggataagt agctttgtaa atatagacga agtagtagat ttatctctat
      361 ttatataccc ggtagaaagc caagttgtac tagaaaacct acgtaaaaaa gtatcacagc
      421 tagaggctgg cctacaaata gatgccgaaa aaggtagggt acgcgaccca ggtaaacaag
      481 ccgccatact agacgcagaa gaactgcgcg ataaactaca ggttggtgaa gaaaagttct
      541 ttcggtttgg tatgtatttt accgtgtatg gcaactcgct agatgaacta gattttgtaa
      601 gccacaagat agaatctatg cttggccaac agttggtgtt tagtaaacct gccagcgccc
      661 agcaagaaca aggtttaaac agcacaattc cacaatttgt agatcagttg caaatcagaa
      721 gaaacatgaa caccggcgcc attggcacaa gttttccgtt taccagtgcc gatttaagtg
      781 acgaccacgg tattttatat ggcataaata tgcacaacag tggcttggtg atatttgacc
      841 gctttagctt agaaaatggt aatagcgttg tatttgctaa atctggtgca gggaaaagct
      901 ttgccgtaaa gctagaggct ttgcgtagcc ttatgttggg tgtagaaata tttatcgtag
      961 acccagaaaa cgaatatgaa cgtatgagtg aagcggttgg tggggcatat gtaaaactca
     1021 gcctaaattc gccaacccgt atcaacccgt ttgacttacc gcttgtcgta gatacagaag
     1081 aagcagataa tgccttgcgc agtaacctta taaccttaca tggactgtta cgtttgatga
     1141 tgggtggtgc acaagcccaa atggccgcca gccaaggttc ggtggttgca ccagcgctta
     1201 acccaacaga agaagcagat ttagacgccg cgctattaga aacctacgct aaagcgggta
     1261 tcaccaacga cccattaacc cataacagta ccccgccaac catccacgat ttatacgaca
     1321 ccttgctgca tatggggggc acaggtccac aacttgccca gcgactacgc aaatatacca
     1381 ctggtacgtt ttctggtatt tttagccagc aaagtaacgt agatattaac aacccgtttg
     1441 tcgtgtttaa tattcgtgat ttagaagatg aactacgccc cgtggcaatg tatattgtgc
     1501 ttaattacat ttggaataaa acaaagtcag atcaaaaaag gcgaatgcta gtaatagacg
     1561 aagcctggca actgatgaaa tacgaagata gcgccagttt tatgtttagc ttagctaaaa
     1621 gagcccgtaa gtataattta ggaatcacca ctatttcgca ggatgtagaa gactttatga
     1681 gttcacgcct tgggcgggcc attgtggcta atgcatctat gcaaatttta ctaaaacaat
     1741 ctgtaagtgc cgtagacgta ttggcagatg tctttaagct cacatcagaa gaaaaaaagc
     1801 gcctcagcca gttcccggtt gggcaaggac tcttctttgc tgggcaaagt cacgtgcata
     1861 tacaaattgc tgccagccca tcagaaacaa gccttataac aaccaaccca gaacaaataa
     1921 aactactcga agaccaaggc caaataccaa tgcgctaaac tccaaaaata agcaaaaaaa
     1981 tgatatacta atgagg
//
LOCUS       DAOPLS010000143         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_66505_length_1996_cov_2.300876, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000143 DAOPLS010000000
VERSION     DAOPLS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66505_length_1996_cov_2.300876"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKV52_03325"
     CDS             complement(<1..447)
                     /locus_tag="PKV52_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99276.1"
                     /translation="MTKKLDDAVNTLQKRFGAGAVTKLGDAPQMIDRFTSGSLRLDYS
                     VLGGGVPRGRIIEIYGPESCLASDTFLQFEVRSSDGQQKNSKGGTIKRLYERFHDVDM
                     GGPKQGRHLQHKENVKFYVKSINEASRVIRNEVLDVVATGVKPVYRV"
     gene            complement(477..995)
                     /locus_tag="PKV52_03330"
     CDS             complement(477..995)
                     /locus_tag="PKV52_03330"
                     /inference="COORDINATES: protein motif:HMM:NF021503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2227 family putative metal-binding protein"
                     /protein_id="HPD99277.1"
                     /translation="MSSGAIHNRVTKQIAVSSGIGVAIIYSNIYYGIALAIGCLLGII
                     ITPDWDIDNGTNANKHMRKVGLGWFLSHLIRPYAVAYKHRSTITHKPIFSTLWRIIYI
                     YVPFVVLVNADQSTNKLTLIYYSSIAQILAFVVWWVTWYFWFMLCYYWLFVGIAVVGL
                     MLSDIAHWLFDL"
     gene            complement(988..>1996)
                     /locus_tag="PKV52_03335"
     CDS             complement(988..>1996)
                     /locus_tag="PKV52_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99278.1"
                     /translation="LNYNFCGAAIATDIIFLIGLTTGFVFNGLMMVLNFWPHLLTHKP
                     KMPAKVKDLLPKPKPTTTPAVVANTEIPDITASTKSPYLQPVAKPASIKVAGKDANLY
                     QFSDMRFDFHRLPHILLAGTSGSGKTVALFNILGTIKRQYPMARFVVIDYGNQDFEQT
                     YPTDLTTFLKVTEAMFLIMKGRQLEGKQTDRTRIVWIVEEFESVLGEIKLEPKTAQTA
                     FYVRLANIGRMARKLSLNMVFVTQSAKAEDLNTSIRNNFANRFIMAMENNSLAGALGC
                     NYDVGSLPTGVGYSSAVKAFIKFPMAKEPPLELIPYGDLIRLANYYKVKYNLIVDEDN
                     E"
BASE COUNT          613 a          447 c          345 g          591 t
ORIGIN      
        1 tacacgataa acaggtttga cacctgttgc aacaacgtct agtacctcat tcctaattac
       61 acgggacgct tcatttatag atttcacata aaatttaaca ttttccttat gttgtaaatg
      121 gcgtccttgt ttggggccac ccatgtctac atcatgaaac cgctcataca ggcgttttat
      181 tgtaccacct ttgctatttt tctgttgccc gtcactgcta cgaacttcaa attgcaaaaa
      241 tgtgtcacta gccaagcaac tttccggccc atagatttca ataatacgcc cacgcggtac
      301 accgccacct aatactgagt agtctagtcg taacgatccc gacgtaaaac gatcaatcat
      361 ttgcggcgcg tccccaagtt tggttacagc acccgcacca aatcttttct gtaaagtatt
      421 tactgcatca tctagttttt ttgtcattgt acaattataa cagattttac aaaattttac
      481 aaatcgaata accaatgtgc gatgtctgat aacatcaagc caacaacggc aatacccacg
      541 aataaccaat agtagcacag cataaaccag aaataccatg taacccacca cacaacaaag
      601 gctaaaattt gtgcaatgct actgtaataa atgagtgtca atttattagt tgattggtct
      661 gcattaacca aaacaacaaa agggacatat atataaatta tgcgccatag cgtactaaaa
      721 ataggtttat gtgtgattgt actacggtgt ttgtatgcaa ccgcatatgg ccgtattaag
      781 tggcttagaa accaacccaa tcccaccttg cgcatatgtt tattggcgtt cgtgccatta
      841 tcaatatccc aatccggggt aataataata cctaacaaac agcctattgc caacgcaatg
      901 ccataataaa tattgctgta aataatggca acaccgatac cactacttac agcaatttgt
      961 tttgtcacac ggttatgtat agccccacta ctcattatct tcgtcaacaa tcaggttgta
     1021 tttcactttg tagtaattag ctaatcggat taagtcacca tatggtatta gctctaatgg
     1081 cggttctttt gccattggaa acttaataaa tgctttgaca gccgaactgt aaccaacacc
     1141 tgtaggtaaa ctgcccacat cgtaattaca acccaatgca ccagcaagtg aattattttc
     1201 cattgccata atgaaacgat ttgcaaagtt gttgcgaata gaagtattca aatcctccgc
     1261 ttttgccgac tgggtgacaa aaaccatgtt caggcttagt ttacgtgcca tacggccaat
     1321 gtttgccaac ctaacgtaaa aagctgtttg ggctgttttt ggttctagtt taatttcacc
     1381 taataccgat tcaaattcct caacaatcca gacaattctc gtgcgatccg tttgtttgcc
     1441 ctccaactgc ctacccttca taatcaaaaa cattgcctca gtgactttca gaaaggttgt
     1501 taagtcggtt ggataggttt gttcaaaatc ttggttcccg taatcaataa ctacgaaacg
     1561 cgccatcgga tattgtcgct ttattgtacc caaaatgtta aataaagcaa ccgttttacc
     1621 actgcccgat gtacccgcca ataaaatatg cggcaaacgg tgaaagtcga aacgcatatc
     1681 gctaaactga tataagttcg catctttacc agcaacttta atactcgcag gcttcgcaac
     1741 gggttgcaaa tacggcgact ttgtactagc cgttatgtca ggtatttccg tattggcaac
     1801 aactgcgggt gttgtagtgg gttttggctt tggtagtaag tccttaactt tagctggcat
     1861 ttttggttta tgggtcaata aatgaggcca aaaatttaat accatcataa gcccattaaa
     1921 gacaaaacct gtagttaagc caataagaaa aataatatct gttgcaattg ctgcaccaca
     1981 aaagttatag ttaagc
//
LOCUS       DAOPLS010000144         1983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_67121_length_1983_cov_4.786307, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000144 DAOPLS010000000
VERSION     DAOPLS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67121_length_1983_cov_4.786307"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKV52_03340"
     CDS             <1..955
                     /locus_tag="PKV52_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPD99279.1"
                     /translation="ISSAKDKNVVIATSNSANDIATTIVSGEYNVVIVDSVQTLATDE
                     ITGVPGSVSQVTNSTQLITLAAKQSNTAVILVGHVTKEGSIAGPKVLEHLVDVVLQLE
                     GDRYAGFRVLRGIKNRFGSTNEAVILEMSDKGLHVVTNPSAALLSERQISDGSIVLAT
                     LEGTRPILVEVQALVNTTSYGYPKRAASGFDLNRLNLLVAMLERRTKLKLSDKDIYIN
                     IVGGIKITENAADLAICMAIASAAKGMQLSTDAVVFGEVGLSGEVRHVPWLEKRIQEA
                     QKMGFTSVIGPRLQKSQTNSGLQYKEAKNIKTILNDTLMKG"
     gene            958..1770
                     /locus_tag="PKV52_03345"
     CDS             958..1770
                     /locus_tag="PKV52_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPD99280.1"
                     /translation="MTDIIIIGLLLLIVVLVYKKPRKKITQPTGGASSVILDSCALID
                     GRVIELAKIGFMPKKIIVPQFIVKELQLLADGRDSYKRERARFGLNVVQQLQNDAELD
                     VTIDETQPGKVTTDENLVALAKLTSAELFTTDYNLNQVATIEGVTVLNVNELAHAVRP
                     VALPGETAHVTILQSGTNRDQGVGYLDDGTMVVVDGAIKDIGKSIQVTINKSHQTVAG
                     KMLFAKKVSQPTIKAKNQTVASKDIKQRPFQQKRTNNTKKRSHIINSHTTTR"
BASE COUNT          650 a          406 c          430 g          497 t
ORIGIN      
        1 tattagcagt gcaaaagata aaaacgtagt tatagcaacc agtaacagcg caaacgacat
       61 tgctactacg attgtttctg gtgaatacaa tgtggttatt gtcgattcag tgcaaacatt
      121 agcaacagat gaaatcactg gtgtgcccgg ctcagtcagc caagttacga acagtactca
      181 actgattact ctggcagcta aacagtccaa tactgcggtt atcttggtgg ggcacgttac
      241 aaaagaaggt tctatcgcag gcccaaaagt tcttgaacac ttagtggatg tcgtattgca
      301 gctagagggt gaccgatatg caggatttcg cgtcttaaga ggcataaaaa ataggttcgg
      361 ctctacaaac gaagcagtca ttctagagat gtccgataag ggtctgcatg tcgttacaaa
      421 tccatcagca gcattacttt cagagcgaca aatcagtgac ggttctatcg tgctcgcaac
      481 cctagaaggc acaaggccaa tactagtaga agtacaagca ctcgttaata caacaagcta
      541 cggctatcct aagcgtgcag catctgggtt tgatctaaac cgacttaatt tgcttgttgc
      601 aatgcttgag cgccgaacca agctgaagct ttctgacaaa gacatttata tcaatatagt
      661 tggaggcata aaaataactg aaaacgctgc cgatttggct atatgcatgg cgattgcttc
      721 agcagcgaaa ggcatgcaac taagtacaga tgctgtggtg tttggcgaag taggtttaag
      781 tggagaggtg cgacatgtac catggctaga aaaacgcatt caagaggccc aaaaaatggg
      841 tttcactagt gttattggtc cacgactaca aaagtcgcaa acgaattctg gtctacaata
      901 taaagaagca aaaaacataa aaacaatcct taacgacacg ctgatgaaag gctaaccatg
      961 acagatataa taattattgg acttctctta ttaatagtag ttttagtata taaaaaacca
     1021 cgtaaaaaaa tcacgcaacc tactggtggt gcaagtagtg ttattttgga cagctgtgcg
     1081 ctgatagatg gaagggtcat tgagctcgcc aaaatagggt ttatgcctaa aaaaatcatt
     1141 gttccacaat ttattgttaa agaactacaa ttattggctg acggccgtga tagctataaa
     1201 cgtgagcgcg ctcgctttgg cttgaatgtc gtgcagcaat tacaaaatga cgctgagcta
     1261 gacgtcacaa tagacgaaac acaaccgggc aaggtaacaa ctgatgaaaa ccttgtagca
     1321 cttgccaaat tgacatctgc tgagttattt accaccgatt acaatctcaa tcaggtagca
     1381 accatagaag gtgtcaccgt gcttaatgtt aatgagctcg cccacgctgt ccgaccagta
     1441 gcgttgccgg gagaaactgc gcatgtgaca attttgcaat ctggtaccaa ccgtgatcaa
     1501 ggggtcggct acctagacga cggtactatg gtcgttgttg atggggcaat taaagacatt
     1561 ggtaaatcaa tacaggtaac gataaataaa tcgcatcaaa ccgtagcagg aaaaatgcta
     1621 tttgcgaaaa aggtttctca accaaccatt aaggctaaga atcaaacagt agcatctaaa
     1681 gatataaaac aacgtccgtt tcagcaaaag cgcacaaata atactaagaa gcgctcacat
     1741 ataatcaata gccatacaac tacacggtag ctacttttaa aacatttatg acgtgtattt
     1801 cagattgatt acacaagcac tagttgtacg ctaatagtgc taaggcagca ctggtgacgg
     1861 agtatcatca ctcgcactcg gtgcagcact caccgtaaag cttgtcgcat cactagcatc
     1921 atataataca gagtctacaa cattagctgt gattgtttgt gtgccagacc cacttggtct
     1981 aaa
//
LOCUS       DAOPLS010000145         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_67183_length_1982_cov_3.980280, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000145 DAOPLS010000000
VERSION     DAOPLS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67183_length_1982_cov_3.980280"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1101)
                     /locus_tag="PKV52_03350"
     CDS             complement(163..1101)
                     /locus_tag="PKV52_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HPD99281.1"
                     /translation="MNKKHTVVLVIVGICLVIVGVVVGKFFVGDTKSTASNTDYPLLA
                     KRLFVENPNDTRINFSPLRKQLNQYFTDNNLKGSLYFEYLPTGTSIRVNGDERYRAAS
                     LMKLPVAMELYKASELGKVDLDSTVTLKKEWLNDGYGTLYSKGEGYQLSLRDAARILL
                     EESDNTALRAILDATDGKLETTDLALGSLDIEFTLNKDTSIDLGTRSYSSFFKCLYFA
                     CYNNKADSQEILNYLSNSTFNNRLQAGIPDDSVTVAHKIGVFNTQVQSDCGIVYYDNG
                     NYLVCVMLMGEDNETTNNQFKNISNVIYRFVSDNKQ"
     gene            1206..>1982
                     /locus_tag="PKV52_03355"
     CDS             1206..>1982
                     /locus_tag="PKV52_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99282.1"
                     /translation="MQKEVKKPKVRLTRKDDGSLGLPTSSQQGSPEQLSHIWGAQKKA
                     QATRRASVKQELSQWRKARVAKKQQQKNQATKSRTKDHKKITNDAQRSVTVRLPHIAI
                     PNKLKNQPKKRLIAVAGIALVVMVTGVVIANVKKSDANNSGEVQGAVTSSIQTNVTPE
                     FPMLTPFGKGVDDLGGFAKISPNGSPPVYAFADSIGSVKIKVSQQQLPDALRTDQAAK
                     LKELANSFNANDTIEIDDNTAYIGASVNGAQSVVYIKGENL"
BASE COUNT          603 a          439 c          404 g          536 t
ORIGIN      
        1 ctcccctcct gtaaatgttt tgccgtgtcg tagcttattc ttcacttctg atccaagcaa
       61 gaggctacga cacggcaaaa catttacagg aggggagcaa cagcctctag acactccttg
      121 taggcaaagt ggtggccaga ttaactgttc gttaccataa aattactgct tattatcaga
      181 gacaaaacga tagattacat tagatatatt tttaaattga ttattcgtcg tttcattgtc
      241 ttcgcccatt agcattacac acactaaata attaccgttg tcataatata caatgccaca
      301 atcactctgg acttgcgtat tgaatacccc aatcttatgg gcaaccgtta ctgaatcatc
      361 gggtatgcca gcttgcaagc ggttattgaa tgtagaatta cttaaataat ttaatatttc
      421 ttgcgaatct gctttgttgt tatagcaggc aaagtacaaa cacttaaaaa acgatgaata
      481 cgaacgagtt cctaggtcaa tgctagtatc tttattgagc gtgaactcta tatctaacga
      541 gcctagcgcc aaatcggttg tttcgagttt tccgtcggtg gcgtctaaga ttgcgcgcag
      601 ggcggtgtta tcggactctt ctaggagtat cctggctgcg tcacgcagac taagctggta
      661 accttcgcct ttgctataga gtgtgccata accgtcattg agccattctt ttttaagtgt
      721 aacagtgctg tctagatcta ccttacctag ctcgctggct ttataaagtt ccatggcaac
      781 cggtaacttc attaaagaag cagctcggta gcgttcgtcg ccattcacgc ggatagaggt
      841 gcccgtaggc agatactcaa aatacaaact gcctttaaga ttgttatctg taaaatactg
      901 gtttagttgt tttctcagtg ggctaaaatt aatgcgcgta tcatttgggt tttctacgaa
      961 caaacgtttc gcaagcaagg ggtaatcggt atttgatgcg gtgctttttg tatcacctac
     1021 aaagaattta cctaccacaa cacctacaat aacaaggcaa atcccaacaa taaccagtac
     1081 caccgtatgt tttttattca tcgctaccat gtattatcac acaaatatag tttgtttaga
     1141 acaggcatca tcgtcgcttg acgtctggtt acactatgaa accagaggtg gtataatgat
     1201 tttagatgca aaaagaggta aaaaagccaa aagttcgctt aacacgtaaa gacgatggta
     1261 gtcttggttt gcctaccagt tcgcagcaag gctcaccaga acaactgtca cacatttggg
     1321 gtgcgcaaaa aaaagcacaa gctacacgac gcgcgtctgt aaagcaagaa ctttcccagt
     1381 ggcgtaaagc gcgcgtagca aaaaaacaac aacaaaaaaa ccaagccacc aaatcgcgca
     1441 cgaaagatca caaaaaaatc actaatgacg cacagcgttc tgttaccgtg cggttgccac
     1501 acatcgcgat acccaataaa ctcaaaaatc agcccaagaa acgacttatt gctgttgctg
     1561 gtattgcctt ggtcgtgatg gtcacggggg tagtcattgc caatgtaaaa aaatcagacg
     1621 caaataatag cggggaagtg caaggtgcag tcacctctag cattcaaacc aatgtcaccc
     1681 ctgaattccc tatgcttacc ccatttggca agggtgttga tgatttagga ggttttgcca
     1741 aaataagccc caacggaagc ccaccagtgt atgcttttgc agattctatt ggttctgtaa
     1801 aaataaaagt gagccaacaa caacttccgg atgctttgcg gaccgaccaa gctgccaaac
     1861 ttaaagagtt agcaaacagt tttaacgcca acgataccat agaaatagac gataatactg
     1921 cgtacattgg cgcttcagtg aatggtgcac agtctgttgt ctatataaaa ggcgaaaacc
     1981 tc
//
LOCUS       DAOPLS010000146         1979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_67344_length_1979_cov_2.897089, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000146 DAOPLS010000000
VERSION     DAOPLS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67344_length_1979_cov_2.897089"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1979)
                     /locus_tag="PKV52_03360"
     CDS             complement(<1..>1979)
                     /locus_tag="PKV52_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99283.1"
                     /translation="VCDQGGRIWLTPDVQLLTDSERNALPTVFDLTNMDESGDVTITR
                     KPQYSTVLTKVSGFYWDGSFTTTETDECPEPPCPDPDPLCSSAPTDLPADDGPTITSH
                     DRQTLKSQAHCNELAGRYFAKENNEYPEFSITTHGNYHGVFDVAYPVFWTTSLTTERF
                     TWTTQKLICRNISLTINTQLGTIRTQVTLEPDAPGEPGVAGYCIDEMPSDDGGDPDVT
                     PVGGGGGVGGRYALVTGASVHYGPGNWQLRTPEDVYDLIQDPFWRAKQNSTDPLDSIL
                     IRCGVGYIKYSNDSGITWHTVTPAVAPPNNLSDTPAPTNIDVTYKFIEPNYLAVDQFV
                     FLVYWANGSGKYRTWLLKTDDFFSSFTWLGIGTGTAPSAPGLTMTNADDYNSLNLGRS
                     SAVQINDSEYAMTFQGGGSTVTGVVYDYDNATDILTKTDVENVTWNNTNHDLRVASTN
                     GFFAVRQYTGSNQPLDIRMASVTGGVLIWEASGTIATRKVSPDPLLRGCKGGAIADVI
                     IAYMATDVVTGDPAIAVQGLTRIGTNIITTGEYYPLISTAGNYFLIEIEKISSYHYVI
                     TYVDEFNQVCASILDGSTMLLTDTVVLDTTPAFPTPLNFTQVEKMIAVPDTNTVAMLY
                     GADSGGGVYRLYMHTYELASANNFGISLDKVSIHTS"
BASE COUNT          515 a          442 c          435 g          587 t
ORIGIN      
        1 gatgtgtgta tagatacttt gtcaagactt atgccaaagt tattggccga tgctaactca
       61 taagtatgca tatacaagcg atatacacca ccacctgagt cagccccata caacatggca
      121 acagtgttcg tatcaggcac ggcaatcatt ttttcaacct gtgtgaagtt tagtggtgtt
      181 ggaaatgcgg gtgttgtatc cagtacaacc gtatctgtta gaagcatcgt gctaccgtct
      241 aagatgctgg cacaaacttg attgaattca tcgacatatg ttattacata atgatacgaa
      301 ctaatctttt caatttcaat taaaaagtag ttaccggcgg ttgatataag cggatagtat
      361 tcacctgtcg tgatgatatt tgtgccaata cgggtcaagc cctgcacagc aattgccgga
      421 tcgccagtca caacatcggt tgccatatac gcaataatca catctgcaat agcaccacct
      481 ttacaccctc gcaatagcgg atcgggcgat actttgcgtg tggcaattgt gccgctggct
      541 tcccaaatca acaccccgcc tgtaacactt gccatacgta tatctaacgg ttgattgcta
      601 ccagtatatt gccgtacagc aaaaaagccg ttagtagatg ctactcgtaa gtcgtgattg
      661 gtgttattcc aagtgacatt ttcaacatct gtttttgtta atatgtcagt tgcattgtcg
      721 tagtcgtaaa caacacctgt tactgtgctc ccaccacctt gaaaagtcat ggcgtattca
      781 ctgtcattta tctgtacagc cgatgatcta cctaagttga gactgttgta atcatcggca
      841 tttgtcattg tgaggcctgg tgcgcttggt gctgttcccg tgccaattcc taaccatgtg
      901 aagctgctga aaaagtcatc tgttttcagt agccatgtac ggtatttacc gctgccgttt
      961 gcccaataaa ccaagaaaac aaattggtct accgccaaat aatttggttc gatgaactta
     1021 taagttacat caatgttagt tggtgcgggt gtgtcgctta gattatttgg tggggcaacc
     1081 gctggtgtta ctgtgtgcca tgtgataccg ctgtcatttg agtatttgat atacccaacc
     1141 ccacaacgta tcaaaataga atcgagcgga tcggttgagt tttgtttagc acgccaaaat
     1201 gggtcttgga ttaaatcgta gacatcttcg ggtgtacgca attgccaatt gccggggcca
     1261 tagtgtaccg aagcacctgt tacaagtgcg tacctacccc caacaccccc gccaccaccg
     1321 acgggtgtta cgtcgggatc accaccgtcg tcactaggca tttcatcaat acaataacca
     1381 gcaacacccg gttcccctgg tgcatcgggt tcaagtgtga cttgggtacg gattgtacct
     1441 aattgtgtgt taattgtaag acttatgttg cggcatatta acttttgtgt tgtccaagta
     1501 aaacgctcag ttgtaagcga ggttgtccaa aatacaggat aggcaacatc gaaaacaccg
     1561 tgataattgc catgtgtggt gatgctaaat tcagggtatt cgttattttc ctttgcgaaa
     1621 taacgccctg ctaactcatt acagtgtgct tgacttttta atgtttgtcg atcatgactt
     1681 gtgattgttg ggccatcatc tgcgggtaaa tctgttggcg cagatgaaca caacgggtca
     1741 ggatcggggc atggcggttc tggacactcg tccgtttcgg ttgtggtaaa actaccatcc
     1801 caataaaaac cagaaacttt tgtcaacact gtactgtatt gtggtttgcg cgttattgtt
     1861 acatcgcctg attcatccat atttgttaaa tcaaatacag ttggtaatgc gttgcgttcg
     1921 ctatcggtta acaattgcac gtcgggtgtt aaccaaatac gcccaccttg atcgcaaac
//
LOCUS       DAOPLS010000147         1977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_67450_length_1977_cov_2.658689, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000147 DAOPLS010000000
VERSION     DAOPLS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67450_length_1977_cov_2.658689"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1977)
                     /locus_tag="PKV52_03365"
     CDS             complement(<1..>1977)
                     /locus_tag="PKV52_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99284.1"
                     /translation="SRKLEVYGGHGDTQLLLHSIGDGGATNTADLMLWASEPGYTYTG
                     VGIANNMYNTTGFPRINTNRGGSMIRLLDNSMRFSTVNSSGTQLDGLVLDGSGNLTAA
                     GVIQGTRFTSTIATGTSPFTVSSTTKVTNLNADLLDGLDSTAFAPASGSGNYIQNQNS
                     SAQSSSNYWISGEGKANNLVVTGTGGVLQFENTATLQAKNSGGTYEQFLWPRWSDNVM
                     YLNYGASGFNIRNNSSTSTMFMGNNGNVSVGTTGSSYRLTVNYTAPATFTNAAGDFNQ
                     MWQAGGTNILGVAASTGDTTARLVTNNGYNLAFHANGAATAHMTLTTAGNLGIGRTNP
                     QYLLDVSKANTSTGAFSSNAQMALRNSDGTTNNWTELAFHGNGSIAAGIAAQNINHGS
                     AYANLSFFTRGASGWSNKLTILSDGNVGIGDTNPAAKLTVNGNILASSNSYLNFGSTS
                     GSAGYGIRDNAGTLQVKNNAGSWQNISAAVWTSSGGALSMVNTTDLLKVNGANAYSYA
                     QSILVNGDLEQWNAANDVPIGYGTFWQLRSTSTRSATHYGNVGSYSAAATFAANPGGG
                     GYQRYDMLDSFDVSPGEFIEVSAYGFYSGTAGGVVPNLSIGILLNDSANDPMFFVPGA
                     AIYTVKDCNLTTSWAECRGTFQIPAGKLKGRIVI"
BASE COUNT          513 a          458 c          433 g          573 t
ORIGIN      
        1 taataactat tctgcctttt aacttgccgg caggtatttg gaatgtacct ctacattctg
       61 cccagctcgt tgtgaggttg caatctttta ctgtgtatat ggctgctcct gggacaaaga
      121 acatagggtc atttgcacta tcgttcaaaa gtatcccaat ggaaagattt ggtacaacac
      181 caccggcggt acctgagtaa aaaccatagg cggacacttc tataaattcg ccgggtgata
      241 catcaaatga atctaacata tcataccgct ggtaaccacc cccacccggg tttgccgcaa
      301 aagtggcagc cgcagaatat gaaccaacat tgccataatg agtggcactt ctggtagatg
      361 ttgagcggag ttgccaaaat gtaccatagc caattggtac atcatttgct gcgttccatt
      421 gttctagatc accgttaact aaaatgcttt gtgcgtagga atacgcatta gcaccattaa
      481 cctttagtag gtctgttgta tttaccatgc tcaacgcccc gcctgacgaa gtccatacag
      541 cagcgctaat attttgccaa gagccggcat tattttttac ctgcaaggta ccagcattgt
      601 ctcttatgcc ataaccagct gaaccactgg ttgagccgaa gttgagatag ctgtttgagc
      661 tagcaagtat gttgccattt actgttaatt tagctgcagg attagtgtcg ccaataccga
      721 cattgccatc gctaagaatt gtcagtttgt tactccagcc gcttgctcct ctagtaaaga
      781 atgaaaggtt tgcgtatgca ctgccatgat tgatattctg tgcagcaata cccgccgcta
      841 tactgccatt gccgtgaaac gctagctctg tccagttatt tgtagttcca tcactgttcc
      901 tgagagccat ttgagcattg ctactaaatg ctccggtaga tgtattggcc tttgaaacat
      961 ccaacaaata ttgtgggtta gttcggccga ttcccaaatt accagctgtg gttaatgtca
     1021 tgtgtgccgt tgctgcaccg ttggcatgga aggctaaatt atacccgttg ttcgtgacaa
     1081 gtcttgctgt ggtatcgccg gtgcttgcgg ctacacccaa aatgttggtt ccacctgctt
     1141 gccacatttg gttaaagtcg cctgcggcat ttgtaaaggt tgcgggcgct gtgtagttaa
     1201 cagttagcct atagcttgac cccgttgtac cgaccgagac attaccgttg ttacccataa
     1261 acatagtgct tgtagaagaa ttattgcgaa tgttgaagcc acttgcacca tagttaagat
     1321 acattacatt gtcgctccac cttggccaca agaactgttc atatgttcca ccagaatttt
     1381 tagcctgcaa ggtagctgtg ttttcaaact gcaatacacc accagtgcca gtaacaacaa
     1441 gattgttcgc tttaccttca ccgcttatcc aataattgct tgaactttgt gcactactgt
     1501 tttggttctg aatatagtta ccacttccac tagccggagc aaaagctgtt gagtctagac
     1561 cgtctagcaa atcggcatta aggtttgtaa cttttgttgt agacgatacg gtaaatggcg
     1621 aagtaccagt tgcaatagta gaggtgaacc tagtaccttg tattacgcca gcagccgtta
     1681 ggttgccaga accatctaag actaagccgt ctagttgggt gcccgatgag ttaactgttg
     1741 aaaagcgcat tgaattatct aacaagcgaa tcattgagcc accgcggttg gtgttaatgc
     1801 gcgggaagcc agtggtgttg tacatattgt tagctatacc aacaccagtg tatgtgtagc
     1861 caggttcgct tgcccacagc ataagatctg ctgtgttagt tgcaccacca tcaccaatag
     1921 aatgaagcaa tagttgcgta tcaccatgac caccatatac ttctaacttg cggctag
//
LOCUS       DAOPLS010000148         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_67814_length_1970_cov_2.549347, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000148 DAOPLS010000000
VERSION     DAOPLS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67814_length_1970_cov_2.549347"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKV52_03370"
     CDS             <1..191
                     /locus_tag="PKV52_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99285.1"
                     /translation="YAVFLVPTAMVYAINPQARAGIPSIMCGFAVLFALILALYILPE
                     IGNKKPLSLAAHDTPKEN"
     gene            complement(203..787)
                     /locus_tag="PKV52_03375"
     CDS             complement(203..787)
                     /locus_tag="PKV52_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD99286.1"
                     /translation="MQDIRKKTQGMSHSNKALVISKAAHAGQTRKGTNQPYIIHPYSV
                     YGNLLPYTNDESTLAAALLHDVLEDVDPTRYSEQHMREDFGNDIVSIVKLVTKDKTIT
                     NWRQSNQSYLDTLWATNDERALLVSASDKLDNLSTSLHDHAREGDTFWARFHAGKEDQ
                     KWWYDSVHQVLVAKIPQHPIVQVYGKKVALLKQL"
     gene            949..1182
                     /locus_tag="PKV52_03380"
     CDS             949..1182
                     /locus_tag="PKV52_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99287.1"
                     /translation="MRDMYHPPVGHHEFEESIEKLLAEEAYDTANNYGYFTSCYSSEA
                     DQIVTDIVTNRLDIVTPNEFVSVRLRVLQEVTV"
     gene            1243..1659
                     /locus_tag="PKV52_03385"
     CDS             1243..1659
                     /locus_tag="PKV52_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99288.1"
                     /translation="MGLLLEYARHTAEDPEPYGTLPESQIPWEPYLFIGIAQENEDMS
                     TSVLNAKNGQALSLDEQLACLRTLRALRSNLSAEAMLNDIYRDTGDESYIAEPQYESN
                     IDGLIPFEPSDYIDGYGCSVCGKDFTYCEHHTGWQN"
BASE COUNT          540 a          425 c          439 g          566 t
ORIGIN      
        1 ggtatgcagt tttcttagtt cctacagcaa tggtgtacgc aattaacccg caagcgcggg
       61 ctggtatccc atctataatg tgcggattcg ctgttttatt tgcacttata ttggcgctct
      121 atatattgcc agaaataggc aataaaaaac cattatctct agctgcgcac gatacaccta
      181 aagagaacta ggcaatcacc cgttacaact gcttaagtaa ggctactttt ttgccatata
      241 cttggacgat tggatgttgc ggtattttag ctacaagaac ttggtgaacg ctgtcgtacc
      301 accatttttg gtcttcttta ccagcgtgaa agcgcgccca aaaggtatca ccttccctcg
      361 cgtgatcatg taaagaagtg cttaagttgt ctagcttgtc gcttgcactg acaagcagcg
      421 cacgctcatc gtttgtcgcc cataatgtgt ctaggtaact ttggttactt tgccgccaat
      481 tggtaattgt cttatccttt gtaacgagct ttacaataga gacaatgtca tttccaaagt
      541 cttcacgcat gtgctgttcg ctgtagcgag ttgggtctac atcttctaga acatcgtgta
      601 gcagcgctgc ggcaagcgtg gactcgtcat ttgtatatgg caacaaatta ccatacacac
      661 tgtaagggtg aataatatac ggttggtttg taccttttct tgtttgccct gcatgcgcag
      721 ctttagaaat aacaagtgcc ttgttggaat gtgacattcc ttgggttttt tttctaatat
      781 cttgcattgt gtcctccttg gccaaaaaat aagaaatcgg cccgaattat tactaatata
      841 attcgtagcc gcaatagacg caggttgtaa ctgccgctgt cctacatcta tattttacca
      901 ctattattaa gattgtaaaa actataaaaa cggtatatta ttctaaaaat gcgtgatatg
      961 tatcacccac cagttggcca ccatgagttt gaagaaagta tagaaaaact attggcagaa
     1021 gaggcctatg acacagctaa taactacggc tatttcacaa gctgttactc tagcgaagca
     1081 gatcagattg tgacagacat agttactaat agactggata tcgtaacacc aaatgaattt
     1141 gtgtcggttc gcttgcgagt tttgcaagaa gtgacggtgt aacagcatgg ccaaacagag
     1201 caagcgaata tttcgctaca agcaaacact agctcgccga ctgtgggtct gctacttgaa
     1261 tatgcacggc atacggcgga ggacccagaa ccatatggca cattgccaga gagtcaaata
     1321 ccttgggaac catacctgtt tattggcata gcccaagaaa acgaagacat gtctactagt
     1381 gtgcttaatg ccaaaaatgg ccaagcatta tcactagatg aacagctggc atgtttacgt
     1441 acattacggg cgctacgttc taacctatca gcggaagcaa tgttgaatga catttacaga
     1501 gatacaggag atgagtcata tattgcggag ccgcaatacg aatctaacat agacggatta
     1561 ataccttttg aaccaagtga ttatattgac ggttatggtt gctcggtttg cggcaaagat
     1621 tttacctatt gtgagcatca tacaggttgg caaaattgaa atacccaaga gggcgcctgc
     1681 tggcatggta gctgcagggc cctcttgggg gtgtgtctat acctaccttc tcaggcgtat
     1741 tgcacgcgga catcatgtgc accgcagaca tgctttgcaa cactgaccaa cacagtttcg
     1801 taggctctta tgcttctgaa gtgtgggcgc ttgtgaagaa aaacctctct atcgctggca
     1861 tagacgcgcg cgtctatttc atgagatctt tctgtcaagg cattgccgta tgatcgggtg
     1921 tgatgctgcg gttcaagagt taggacatag gagccctctt ctcctttact
//
LOCUS       DAOPLS010000149         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_67897_length_1968_cov_3.700470, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000149 DAOPLS010000000
VERSION     DAOPLS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67897_length_1968_cov_3.700470"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..485)
                     /locus_tag="PKV52_03390"
     CDS             complement(24..485)
                     /locus_tag="PKV52_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPD99289.1"
                     /translation="MKITIITVGKKHDTAIKTLIETYQQRIQAAMTLEWRYVSPSSLA
                     AQAARKQESQKLLQIVKPSDMVWLLDERGEQISSPALAAKLNVMQLHAVRNIAIIIGG
                     AYGVDDTVHSRASWVWSLSSLVFPHQLVRLLLVEQLYRATQINKGTGYHHA"
     gene            complement(486..668)
                     /locus_tag="PKV52_03395"
     CDS             complement(486..668)
                     /locus_tag="PKV52_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99290.1"
                     /translation="MASWVAALLFAIGGSAWLYNVVMKQTGSNTRASIVVTILGFVFM
                     FVIFWSVFDFVAGMAN"
     gene            complement(668..1072)
                     /locus_tag="PKV52_03400"
     CDS             complement(668..1072)
                     /locus_tag="PKV52_03400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPD99291.1"
                     /translation="MQDSIFTKIIKGEIPCHKVYEDAKTIAFLDIYPIQPGHVLVVPK
                     TQVDHFDDLSPEDYAALFNTVQKVAKQVKSALSATRACLRVEGFDVPHAHVHVIPCNV
                     ANDFYNANRMQQEPDHARLAAMAQKLYIKGGE"
     gene            complement(1075..1743)
                     /locus_tag="PKV52_03405"
     CDS             complement(1075..1743)
                     /locus_tag="PKV52_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPD99292.1"
                     /translation="MNQIYCSNCGNLVPASANYCTQCGAATHGHASAVFRAQEPPVEH
                     PQLVRQLPKQALREHVAYIPKQNLQGDGIFFFFVSYFGKTFLMFMLLLVGAALQPYPF
                     VFGLMGYFLILLLTSLFVYNNFSYEINADGLKIESGVIHKRQVSLPYEQIQNVNIERT
                     VLDRIFGFARISIETAGSATGAPIDTGVLKSKSEAYIPGLHIDQAKKVHDLLIDGADG
                     SVED"
     gene            1815..1904
                     /locus_tag="PKV52_03410"
     tRNA            1815..1904
                     /locus_tag="PKV52_03410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1849..1851,aa:Ser,seq:cga)
BASE COUNT          553 a          422 c          426 g          567 t
ORIGIN      
        1 atgggcaaag acacaaccta gtattaggcg tgatggtacc cagtgccttt gtttatctgt
       61 gtggccctgt agagctgctc taccagcaat aaacgtacta attggtgtgg gaatactaac
      121 gaagacaaag accacaccca gctcgctcgt gagtgcacgg tgtcatcaac accatatgca
      181 ccgccaataa tgatggctat gttacgcacg gcgtgtagtt gcataacatt tagcttggct
      241 gcaagggcag gtgagctgat ttgctcaccg cgctcgtcca gtagccacac catatcactc
      301 ggtttaacaa tctgtaacag tttttgtgat tcttgcttgc gggcagcttg ggctgcaagt
      361 gagctgggtg aaacataacg ccattctagt gtcatagcag cttgtatgcg ctgttggtac
      421 gtttctataa gtgtttttat ggctgtatca tgctttttac caacagtgat gatggtaatt
      481 ttcatctagt ttgccatgcc agctacaaag tcaaaaacag accaaaatat tacaaacata
      541 aacacaaacc caagtatcgt tactacaata gaagctcttg tgttgctacc tgtttgcttc
      601 atcacgacat tatatagcca tgcgctaccg cctatggcaa ataaaagtgc agctacccaa
      661 gacgccatta ttcaccccct ttgatataca atttttgagc cattgcagca agacgagcat
      721 gatcaggctc ttgttgcatt ctatttgcat tataaaaatc attggctaca ttacaaggaa
      781 taacatggac atgagcatgc ggtacatcaa acccttcaac acgcaaacaa gcacgggtgg
      841 cagaaagtgc cgattttact tgtttcgcaa ccttttgtac ggtattaaat aacgcggcgt
      901 aatcttctgg gcttaaatca tcaaaatggt ctacttgtgt tttgggtact acgagtacgt
      961 ggcctggctg gatagggtag atatctaaaa aagctatagt tttggcatct tcatacacct
     1021 tgtggcaggg tatttcgcct ttaataattt ttgtaaaaat actgtcttgc attgttaatc
     1081 ctctacgctg ccgtctgcgc catctattaa taaatcgtgc acttttttgg cttggtctat
     1141 atgaagaccg ggtatgtagg cttctgattt tgatttaagc acccctgtat caattggtgc
     1201 tcctgttgcg ctaccggcag tttctatact aatacgtgca aaaccaaata tacggtctaa
     1261 cacggtacgt tctatgttga cgttttgtat ttgctcgtac ggcaaagata cttgtcgttt
     1321 atgaatcaca ccagattcta tctttagacc atctgcgtta atttcgtagg aaaaattatt
     1381 atacacaaat aaagacgtaa gtaagagtat taaaaagtag cccataagac caaatacaaa
     1441 agggtacggc tgtagtgcgg cgcctaccag caacaacata aacattagaa aagttttacc
     1501 aaaatagctt acaaagaaga agaatatacc atcaccctgt aagttctgct tgggtatata
     1561 tgcaacatgt tcacgcaacg cctgtttagg gagctgacgc acgagctgcg gatgctctac
     1621 gggaggttct tgagctctaa aaactgcaga agcgtggcca tgagttgctg caccacactg
     1681 ggtgcaataa ttggcactcg ccggcactaa attgccacag ttactgcaat atatttgatt
     1741 catatgtaca gtttatcact ttggggcgtt ggtgcttgaa ataattgtca tgacagatga
     1801 taataaacag ccgaggagag gtgcaagagt ggttgaattg gcttgtctcg aaaacaagtg
     1861 agccagcaat ggttccgagg gttcgaatcc ctccctctcc gccaatttgt gagccccgct
     1921 tttatgcggg gttttcaaat tgctgtgaaa agtgcattcg aacccgat
//
LOCUS       DAOPLS010000150         1958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_68369_length_1958_cov_3.622701, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000150 DAOPLS010000000
VERSION     DAOPLS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68369_length_1958_cov_3.622701"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKV52_03415"
     CDS             complement(<1..180)
                     /locus_tag="PKV52_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99293.1"
                     /translation="MSSSASFLLIVILVVIPSLAGGMLFFARKNLREAKNYERGLKMV
                     PLLIHLPPSSDDLESK"
     gene            complement(196..630)
                     /locus_tag="PKV52_03420"
     CDS             complement(196..630)
                     /locus_tag="PKV52_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99294.1"
                     /translation="MDGMQTPPQHELPKPHVSNGEALPDFGKEGFGHVTPELKVAPMP
                     GERLSAANSAVSQAVSAVAVPAMPLQQLQPSTDPQSTAAVIADDNDVIEKEWVDKAKE
                     IVVRTQGDPHAKSNQLTGLKKEYIQQRYGKTIAIPKEDTSKK"
     gene            708..1031
                     /locus_tag="PKV52_03425"
     CDS             708..1031
                     /locus_tag="PKV52_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99295.1"
                     /translation="MIVANNINKSSTVACRSEANIIRAGGTHANKKVIVIWLYQLFPV
                     YKAKNKAITLADNTYTKINIIKTGTAVSVFGVILPAKIKNIVDKLNKLVVRNNICFFI
                     LLTIP"
BASE COUNT          584 a          374 c          348 g          652 t
ORIGIN      
        1 tttgctttct agatcgtcac tacttggtgg taaatggata agcaaaggca ccatttttaa
       61 gcctctctcg tagttctttg cttctcgtag gtttttcctt gcaaagaata acattccacc
      121 agctaaactg gggattacca ccaaaataac tataagtaaa aatgaggccg aactactcat
      181 gtcgtaccat atttattact ttttagacgt gtcttctttg gggatagcaa ttgtttttcc
      241 atagcgttgc tgtatgtact ctttctttaa accagtcagc tgattagact ttgcatgtgg
      301 atcaccttgt gttcgaacga caatttcttt agctttatct acccattcct tctcaatcac
      361 atcgttatca tcagctatca ccgctgctgt actctgaggg tcggtactcg gctgaagctg
      421 ctgcaacggc atagcaggta ctgcaaccgc acttaccgct tgggaaacgg cgctatttgc
      481 tgcagataaa cgctcacctg gcatgggagc aacttttaac tctggtgtta catgaccaaa
      541 accctctttg ccaaaatctg gcaaggcctc tccgttagat acgtgaggtt ttggcaactc
      601 gtgttgcggt ggtgtttgca ttccatccat cagaggttag tatatcaaaa taaagagttt
      661 atgtttatac catttatttt ttatatgtgg cataccatgc tatcaatatg attgttgcaa
      721 ataatataaa taaatctagt actgttgcct gccggagcga agcaaacata ataagagctg
      781 gtggtacgca tgcaaataag aaagtcatag tgatatggct ataccaactc tttcctgtat
      841 acaaagcaaa aaataaggct ataacattag ccgataatac atatactaag attaatataa
      901 tcaagacagg tacagctgta tccgtttttg gtgtaatact gccagcgaaa atcaaaaata
      961 tagttgataa gcttaataaa ctagtcgtaa ggaacaatat ctgttttttc atacttttaa
     1021 ccatacctta agtttctcat aaaagtgaat atccgccccc aaccaggcgg atattcacca
     1081 acaacgtaaa atgtatactt ggagttctga gacggtaaat ccgctctgta aaattactat
     1141 ttagcgctta ccgtctggca gaactttgac tgaccgctct ctctgccttt agggcttcga
     1201 gggcgttaac gaacacttgt ttgtacattt tgtaaggttt tttatgtttt tcttacatgt
     1261 atgaagcgta gcaaaataat gcattttagt caagtataac gtagttatta ttactacttt
     1321 catctgtata ttagttaatt attttgttgt tataaatata acattttttc tgtatacata
     1381 attaaattgt ttatatgtat gctgctttca ggtgctttta tggtatggcc gatagatatt
     1441 tcgtaggact acgtttatac gttctagcga atctctatag gttgttcatt atgcacacaa
     1501 cactacaaag attaaggttt ttattgtgtg tgattacgaa gcagaaacaa ccaccacggt
     1561 ttgttattat gagcgataac ttacccttca aaaacatatt aatacgtcta gcagtagctg
     1621 tatttaaaat atgcagtttg tattaattac ctaaaattat aaaaaagtca ttgacagcaa
     1681 ttttgcttat cctttactat gaacttagcg cttgaataaa aaaagtgctc aaaaataaaa
     1741 acctggcttt aataacaacg gcttctcgca agccgttgtt ccaagcaaat caggttttac
     1801 acagacacaa aaacctatat accgtcatca cccgcagctt gatgacggta ttttttattc
     1861 atactcatat tgttgtacta tagggggtat gattaaatca cctattagta ttggctacac
     1921 ttcggttgcg gtacatgtag aatgtagcac ttctaatg
//
LOCUS       DAOPLS010000151         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_69102_length_1944_cov_3.214929, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000151 DAOPLS010000000
VERSION     DAOPLS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69102_length_1944_cov_3.214929"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1944)
                     /locus_tag="PKV52_03430"
     CDS             complement(<1..>1944)
                     /locus_tag="PKV52_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99296.1"
                     /translation="HTINGGGTTGGMGAGYATVYNNILYYVGGFDGRNWSKKIYYVGV
                     NQATGALTASWTAAVGTIPRSMGNNFAFTRNGFLYNIGGCWNGKAGSAGCNHYSASVV
                     KCTITAATGAIASGACVTTGQLQLGAAMGTMAGAVAGNFIYLAGGASSTYNTSTTVYR
                     AEINNSNNIVRIDNGAATGGWQTTTASLTETRRRAFAAAVNGYLYVIGGHNGTTNTTV
                     SDTQIGKIDTETGDITSFTVVSNAITARWDLRGAAANSTMYLLGGCNAGIPPGSCTTL
                     NGNSEYLLAYNNYNGTPAGYTNTSAITSARVGASSVIYNGYIYFAGGCTATACNGSTV
                     RTQVDYAPINDDGTIGTWATTSSMGTNRSFFKLVANNGYMYAIGGSANGSSNSSTEHA
                     TINANGTLGAWSTDTSLPTGLGGIDGVIFNGYVYVTGGKGNGAVGYVSTVYYSQINTA
                     TGALGTWTTGAAFTGGRNNHTALAYNSALYILGGFDGTNYLADVQYAKINADGSIGSW
                     NYTANLPQGLQMSSGFAANGYLYLISGATGASACSAATYVASIHANTTIANGNNPTGL
                     GGWSQTSVVIGSARYGSASAYDGGKAYVLGGACGTTMVPSGATQNYYSTIQSQPQVAK
                     YSYYIDANNDVFPSKVLYNGIDNG"
BASE COUNT          408 a          503 c          569 g          464 t
ORIGIN      
        1 ccgttgtcta taccgttata gagcactttg gacgggaaga catcgttgtt agcatcgatg
       61 tagtacgagt atttagccac ctggggctgc gactggatgg tggagtagta gttttgggtg
      121 gcaccactgg ggaccatggt cgtaccgcat gcgcctccta agacataggc tttgccgcca
      181 tcgtaggcac tggccgagcc gtagcgtgcg ctgccaataa ccacactggt ctgtgaccat
      241 cctcctaggc ccgtagggtt gttcccgtta gcgatagtgg tgttggcatg aatcgatgct
      301 acatatgtgg ccgcagaaca ggctgatgca ccggtggcgc cacttatcaa atataggtag
      361 ccattggctg caaagccact cgacatctgc aggccctgcg gcaggttggc ggtatagttc
      421 cagctaccga tggagccatc ggcatttatt ttggcgtatt gcacatccgc caaatagttt
      481 gtaccgtcaa atccgcccag gatgtagagg gcgctgttat acgccaacgc cgtgtggttg
      541 ttccggccgc ccgtaaaggc ggcgcccgta gtccaggtgc caagcgcccc tgtagcggtg
      601 ttaatttgtg aataatacac ggtagacaca taacccacag caccattacc cttaccgcca
      661 gttacgtata catagccgtt aaagataacg ccgtcaatac cgcccaggcc agtggggagc
      721 gatgtatcgg ttgaccaggc gccaagcgtg ccattggcat taatagtagc gtgttcggta
      781 ctggagttag atgagccgtt tgcggatccg ccaatagcat acatgtaacc gttgtttgca
      841 acaagcttga aaaacgagcg gttagtaccc atggagctgg tggtggccca ggtgccaata
      901 gtgccgtcat cgttaattgg tgcgtaatca acttgcgtgc gcactgttga gccgttgcag
      961 gccgtggccg tgcagccgcc cgcaaaatag atatagccgt tgtatataac agagctagca
     1021 cccacacgcg cgctggttat ggcgctcgtg ttagtatatc ccgccggcgt gccgttgtag
     1081 ttgttgtagg ccagcaggta ttcgctgttg ccgtttagag tggtgcagct gcccggcggg
     1141 ataccggcat tgcagccgcc gagtaggtac atggtgctgt tggcggcggc gccgcgtagg
     1201 tcccagcgag cggtaatggc gttagacaca acagtaaagc tagtgatgtc gccggtttca
     1261 gtgtcgattt tgccaatctg agtgtcggat acggtggtgt tggtggtacc gttgtggccg
     1321 ccaatcacgt acaaataacc gttcacagcg gcggcaaagg cgcggcggcg cgtctctgtt
     1381 aggctggccg tagtggtttg ccagccgccg gtggcggcac cgttatctat tctgactatg
     1441 ttgttactgt tgttgatttc tgctcgatac accgtagttg aggtgttata ggtgctactg
     1501 gcgccgcccg ctaaatagat aaagttaccc gccacggcac ctgccatggt acccatagca
     1561 gccccgagtt ggagttggcc agtggtaacg caggcgccgc tagcaatggc gccggtagcg
     1621 gctgtaatgg tacatttaac aacgctggcg ctgtagtggt tacagcctgc ggaaccagcc
     1681 ttgccgttcc agcagccgcc aatgttatac aaaaagccgt tccgggtgaa cgcaaagtta
     1741 ttgcccatgg atctgggaat tgttccaacg gcagctgtcc agctagctgt tagcgccccg
     1801 gtggcctggt taaccccaac atagtagatt ttcttggacc agtttctgcc gtcgaagcct
     1861 cctacgtagt acaaaatgtt gttatagacg gtagcgtaac cagcacccat accaccggta
     1921 gtgccaccgc cgttaatggt gtgg
//
LOCUS       DAOPLS010000152         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_69310_length_1940_cov_2.946419, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000152 DAOPLS010000000
VERSION     DAOPLS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69310_length_1940_cov_2.946419"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..952
                     /locus_tag="PKV52_03435"
     CDS             170..952
                     /locus_tag="PKV52_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD99297.1"
                     /translation="MIYSNKLNGYGLLYGQGQNDCDAQYVPWFDDRTDYVSALEETGL
                     LLSGEPGAGKSHIAEDVLQDSLRFGKDCLQIICHINGSSEKGRSETQKLVTQLAQAKE
                     NGVLVLDNADYVVYTGGRRKKISNAKSARYRSFLRNVIETSYDAGTHIFATSHTAMWR
                     QNHSSIPETEDAFYGIFPRGAVVEKEFTGSMTASNVYRILCLRGVESTKAQQITNVLQ
                     SLGSLSFRNAYHIETQETTPEAVAEAVHKVDDIKNQKIIGGR"
     gene            complement(1023..1098)
                     /locus_tag="PKV52_03440"
     tRNA            complement(1023..1098)
                     /locus_tag="PKV52_03440"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1063..1065),aa:Trp,seq:cca)
     gene            1188..1853
                     /locus_tag="PKV52_03445"
     CDS             1188..1853
                     /locus_tag="PKV52_03445"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD99298.1"
                     /translation="MSKTKLKRPVLIYLYGIPGSGKSLLSHNLSNELDIVVVSSDRLR
                     YELFEDPRHDKTEIQIIGQLMNYMTEEFLKAGKSVIYDMSVSRFADRKELRELAKKNH
                     AKDLMIWLQVDIETAWARSKNRDKRKAEDKYASVLSPDQFKQYVHIMQNPRNEDYVVV
                     SGKHLFPNHKSIIVRKLLELGVAESDDAAFKVAKPELVNLVSKAQAQAGRVDYSRRNI
                     SIQ"
BASE COUNT          690 a          355 c          398 g          497 t
ORIGIN      
        1 cactatatcc gtacaaacac acctattgcg cgtaatgcaa ataagtaaaa aacgttaagt
       61 acacatagaa cgtataccta ggctactttt taatatgtta taatatatag taatatttaa
      121 gtaaattaaa cactagcaat ttaccaattg cgcaagtatt aaaccgtcta tgatatatag
      181 taataaactt aatggttatg gcttattgta tggccaaggc caaaatgatt gcgatgcaca
      241 atacgtgccg tggtttgatg atagaacaga ctatgtaagt gcgctagaag aaactgggtt
      301 actactatca ggtgaaccag gtgctggtaa gagccatata gcagaagatg tgttgcaaga
      361 tagcctgcgc tttggtaaag actgcttaca gattatttgc catattaatg gttctagcga
      421 aaaaggcaga tcagaaactc aaaaattagt tacacaacta gcacaagcta aagaaaatgg
      481 agtgcttgtt ctagataatg cagattatgt tgtatatact ggcgggcgcc gtaaaaaaat
      541 atctaacgca aaatctgccc gctatagaag ctttttaaga aacgttatag aaacgtctta
      601 cgatgcaggc acccacatat ttgctactag ccacactgct atgtggcgcc aaaatcatag
      661 cagcatacca gaaacagaag atgcttttta tggtatattt cctcgtggcg cagttgtaga
      721 aaaagagttt accggcagca tgactgcaag taatgtctac agaatattat gcttacgtgg
      781 cgtagagtct accaaagcac agcaaataac taatgttttg caatcactgg gcagtttgtc
      841 tttccgtaac gcctaccata tagaaacaca agaaactaca ccagaagcag tagcagaagc
      901 tgtacataaa gtagatgata ttaaaaatca aaaaataata ggtggccggt aattacatta
      961 cagcagctgg tagtaaaaaa atacccagaa aaagcatcat aaaaagcgat aatatcaaaa
     1021 cgtggcaggg gagacagggc tcgaacctgc ggccactggt tttggagacc agtgctctac
     1081 caactgagct actcccctaa atgcgaataa tttacgctag catattatag cacgctactc
     1141 tagacgacat ctacaacaat tggctaaaat atggtatcgt ataatggatg tcaaaaacga
     1201 aattaaagcg cccagtacta atttatttgt atggcatacc aggttctggt aagtctttgt
     1261 tgagccataa tctaagcaac gagctagaca tagttgtagt tagcagcgac cgcttgcgct
     1321 atgaactatt tgaagaccca cgtcatgata aaaccgaaat tcagattatt ggccagttga
     1381 tgaattacat gacagaagaa tttttaaagg ctggtaaaag tgtaatttac gatatgtctg
     1441 ttagtagatt tgcagacaga aaagaactcc gtgaactggc caagaaaaac catgcaaaag
     1501 atctaatgat ttggctacaa gttgatattg aaacagcgtg ggcacgcagt aaaaaccgtg
     1561 ataaacgtaa agcagaagac aagtatgcat ctgtactaag tcctgatcag tttaagcagt
     1621 atgtacacat aatgcaaaac ccacgcaacg aagattacgt ggtagtaagt ggcaagcatc
     1681 tattccctaa ccataaatca attattgtta gaaaactgct tgagctaggc gttgcagaaa
     1741 gtgacgatgc agcgtttaaa gttgccaaac cagaactagt taatctggtg tctaaagcgc
     1801 aagcacaagc tggcagagta gattattcgc ggcgcaacat atctattcaa taaagtatgg
     1861 caacaacaaa aagcgaatat atcgctgata ttggacacgt tcgtataaca aaaaagcggg
     1921 gtgtcaaacg tatttctatg
//
LOCUS       DAOPLS010000153         1933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_69656_length_1933_cov_3.684771, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000153 DAOPLS010000000
VERSION     DAOPLS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69656_length_1933_cov_3.684771"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKV52_03450"
     CDS             complement(<1..673)
                     /locus_tag="PKV52_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPD99299.1"
                     /translation="MLHFFNTRTGHIEPFKAMNDKEVLLYTCGPTVYSSLQIGNWDSF
                     LRWDVLVRLLQARGYKVTRVMNITDVGHLVSDADDGEDKMQKGARKEGTTAWAIAEKY
                     TNEFIEGMNTLGCTVPTYYTKATEFIDEQIELIKTLEEKGYTYVIDDGVYFDTSRFPR
                     YADFARLKLEDNQEGARVEANRQKKSASDFALWKFSPVNKQRDMEWNSPWGKGFPGWH
                     LECSAM"
     gene            complement(678..1679)
                     /locus_tag="PKV52_03455"
     CDS             complement(678..1679)
                     /locus_tag="PKV52_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPD99300.1"
                     /translation="MSHKTTDTQVKEILESAQTVLVIQSDRPDGDSLASALYIEEVLE
                     QRGKKVMLYCGVAIPEYLRFIEGWDRVTNELPRSIDATIMVDNAALVLLEKFDQSPDA
                     MFIKTKPFIIADHHAAVMSDIPYATHNISRDGYASAGELLYEVFTEYAYQSNLRAKQL
                     VMQSILSDTLGLTNDLATPRTYRLMADFIEAGVDRVGLEEARRAYSKMDERVFRYKGQ
                     LIQRTEFLFDGAVALCVITEAEAYDVGTLYNPGPLILSELLMVKNVRVALAIKTYHNR
                     ITVAIRCTHGTPLAHELAKKFGGGGHAYSAGFRLDENNLSLSDATANIVSALKELLK"
     gene            complement(1676..>1933)
                     /locus_tag="PKV52_03460"
     CDS             complement(1676..>1933)
                     /locus_tag="PKV52_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPD99301.1"
                     /translation="LDGIGPEQLHIQELVDRIKRDNVKEVIVATNASVEGETTALYIQ
                     EALKASDIALTRLARGLPIGVDLEYADQITLTRALQGRTAL"
BASE COUNT          505 a          442 c          364 g          622 t
ORIGIN      
        1 ccattgcact gcattcaaga tgccacccag ggaagccttt tccccatggg ctattccatt
       61 ccatatcacg ttgtttattt actgggctga atttccaaag agcaaagtca cttgccgatt
      121 ttttctgccg atttgcttca acacgtgcac cttcttggtt gtcttcaagt tttagcctgg
      181 caaagtcagc atatcgtgga aatcgactcg tatcaaaata caccccatca tcgatgacgt
      241 atgtatagcc tttttcttca agcgttttaa tcagttctat ttgctcatca atgaattcgg
      301 ttgcttttgt ataataggta ggcacagtac agcctagcgt attcatacct tctataaatt
      361 catttgtata tttttctgcg atcgcccaag ctgttgtacc ttctttacgt gcaccttttt
      421 gcattttgtc ttcaccatca tctgcgtcac tgactagatg accgacatca gtaatattca
      481 taacgcgtgt aactttgtag ccccgagcct gcaatagtct cacgagtacg tcccagcgta
      541 aaaaagaatc ccagttgcca atttgtaatg aactatatac tgtaggtccg cacgtatata
      601 acaacacttc tttatcgttc attgctttaa aaggttctat gtggccagta cgtgtgttaa
      661 aaaagtgtag catgtaatta ttttagcagc tcttttaatg cagagacgat gtttgcagta
      721 gcatcagaca gactaaggtt gttttcgtct agacgaaagc ccgccgaata ggcatgaccg
      781 ccgccaccaa atttctttgc caactcatgc gcaagtggtg ttccatgtgt gcaccgtata
      841 gctacggtaa tacgattgtg ataagttttt atagcaagcg ccactcgtac gttctttacc
      901 ataagtagtt cgcttaatat taaaggtcct ggattgtaca atgtgcctac atcatatgct
      961 tctgcttcgg taatgacaca gagcgctaca gcaccgtcaa ataaaaactc agttctttgt
     1021 atcagctggc ctttataacg aaagactcgt tcatccattt tgctatacgc acgacgagct
     1081 tcttctaacc ctaccctatc tacgccagct tcaataaaat cagccattaa gcgataggtt
     1141 cttggtgtcg ctaaatcatt tgtgagccca agcgtatcag aaagtatact ttgcattact
     1201 aattgcttag cacgcaggtt gctttggtat gcatattctg tgaatacttc atagagtagt
     1261 tcaccagcag aggcatagcc atcgcgactt atattgtggg tcgcatatgg aatatctgac
     1321 attacagcag cgtgatgatc tgcaataata aaaggcttag ttttaataaa cattgcatct
     1381 ggcgattgat caaatttttc taataaaacg agcgcagcat tatctaccat aattgttgcg
     1441 tctatgctac gtggtagttc atttgtgact ctatcccagc cttctataaa acgtaagtat
     1501 tctggtatag ctacgccaca ataaagcatt acttttttgc cccgttgttc tagcacttct
     1561 tctatataaa gtgctgacgc taatgagtca ccatctggcc tgtctgactg tattactaaa
     1621 accgtttggg cagattcgag aatttctttt acttgagtat cagtcgtctt atggctcata
     1681 atgccgttct tccctgcaaa gctcgggtta aagtgatttg atctgcatat tcaagatcca
     1741 cacctatagg tagcccccgt gctagtcgcg tgagtgcaat atcactagct ttcagcgctt
     1801 cttgaatata taaagcggta gtttcccctt ctacactcgc attcgtagca acgatgacct
     1861 ctttgacatt gtcacgcttg attctatcaa caagttcttg tatatgtagc tgctcagggc
     1921 cgataccatc tag
//
LOCUS       DAOPLS010000154         1930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_69825_length_1930_cov_2.922667, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000154 DAOPLS010000000
VERSION     DAOPLS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69825_length_1930_cov_2.922667"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PKV52_03465"
     CDS             <1..954
                     /locus_tag="PKV52_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPD99302.1"
                     /translation="TGAFTCRQKAEKSLDAGTWINLSKSEVTLSPRTTELVDFSIAVP
                     NKTDVGEHNACIVFAAKDDVEKVGGSLTIRTRSAIRVAITIPGELKRGVNIQSFDVKQ
                     NNDVQTFTVLMRNTGNVSADTNVDVQMKSLFGKVAYTNHGQYPVLSGNSLEVNYDNKK
                     TPFWGGFYLADATLSYNKDANKWDTKAKNTLQTEKSGTKIIFVIPQLPAIIVYFVIIL
                     VVSGVVAYWWFEKRKVQLILDTWQHHKIKKGDTLDSLSTERKANWHLVAKVNKLKAPY
                     TLKVGDSILLPRRPRKSSDTAIQSKSKTIKTNKTTVKSKEP"
     gene            1067..>1930
                     /locus_tag="PKV52_03470"
     CDS             1067..>1930
                     /locus_tag="PKV52_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99303.1"
                     /translation="MTTTLDPNGTVTGNWTGGGTADGSCTGNCQRVDDGSTPDGDTSY
                     VSSSAANVDEYSLPDTVGGQRATSIKLRVYMKRQSAESCFWNWGTPSTNLLQMNIRIG
                     GTLQTQSNKTPTANYAWYEQDYIGTWTQSQINSLQIWVSKSTTTGCVLRNNNATFRIS
                     AVQAVVSWTSPDLNQESSRVYLNTDNATPGTPIATSNSLSDVQHDTAFRVRMAVKPTA
                     VDWQTDTWGPHSNTYKLQYSSKSASSCAAQSSGWGDVSSGSGNIRWYNNPGVADGAAI
                     TAYANDPTVGGS"
BASE COUNT          609 a          370 c          444 g          507 t
ORIGIN      
        1 acaggggcct ttacttgtcg ccaaaaagca gaaaaaagtc tcgatgctgg cacatggata
       61 aatctatcga aaagtgaagt taccttgtca ccaagaacaa ctgaacttgt agattttagt
      121 attgcagtgc caaacaagac cgatgttggt gagcataatg cttgtatcgt atttgcagct
      181 aaagacgatg ttgaaaaagt aggcggtagt ttgactatcc gaacaagatc ggcgataaga
      241 gttgcaataa caattccggg tgagctaaag cgtggtgtta atatacagag ctttgatgtt
      301 aagcaaaaca atgacgtaca aacctttaca gtgcttatgc gtaatactgg taacgtttcg
      361 gctgatacaa atgtagatgt acagatgaag tcattgtttg gtaaagtggc ctatactaac
      421 cacggccaat atcctgtact atctggaaat agtcttgaag tgaactatga caacaagaaa
      481 actccatttt ggggtggttt ttatttagct gacgccacgt tgtcttataa taaagatgcc
      541 aacaaatggg acacaaaggc aaaaaacaca ctccagacag aaaaatctgg tactaaaata
      601 atatttgtga tcccgcaact gccagctatt atcgtctatt ttgtaattat attggtagta
      661 tcaggagttg tggcttattg gtggtttgag aaacgaaaag ttcaattgat tcttgataca
      721 tggcaacatc ataaaattaa aaagggtgat acacttgact ctctatctac cgagcgaaaa
      781 gcgaactggc atttggttgc aaaggttaat aagctaaagg cgccatatac gttgaaagta
      841 ggtgatagca tactattgcc tcgccgaccg aggaaaagta gtgacacggc aatacaaagt
      901 aaatcaaaga caataaaaac aaataaaaca actgtaaaat ctaaagagcc ataggtcttt
      961 gatgtttcgt atacgcctca aacatactaa aactagatta attgtcattg ttattctagc
     1021 tagtattagt atagtatttc ttacggctac ggcttgggcg ttgacattga ccacaacttt
     1081 agaccctaat ggaacagtta ctggtaactg gacaggcggt ggaacggcag atggttcgtg
     1141 tactggaaac tgtcaacgag ttgatgatgg tagcaccccc gatggtgaca caagttatgt
     1201 atcaagctca gctgcgaatg tcgatgagta ctcactacca gatacagttg gcggccagcg
     1261 agctacgagt ataaagttac gggtttatat gaagcgacag tcggctgagt catgtttttg
     1321 gaactggggc actccgagca caaacttact gcagatgaat attcgcattg gcggcacttt
     1381 gcaaacgcag tcaaataaaa ccccgacagc taactatgct tggtatgaac aagactacat
     1441 aggcacctgg acgcaatcac agataaatag cttacagatt tgggttagta agtctactac
     1501 aactggctgt gttcttcgta ataataatgc gacttttcga atctctgccg tgcaagcagt
     1561 tgttagctgg acttcaccag atttgaatca agagtcgagc agagtttatt tgaatacaga
     1621 caacgctacc ccaggaacgc caatagctac gtcaaatagc ttatctgacg tacagcatga
     1681 tacggctttt cgagttcgaa tggcagtcaa gccgacggcg gtagattggc agactgatac
     1741 ttggggccca catagtaaca cttataaatt gcagtattct tcgaaatctg catcaagttg
     1801 cgccgcacag tctagtggct ggggtgatgt gtctagtgga tcgggcaata tacgttggta
     1861 caacaatcca ggcgtagccg acggggcagc tataaccgcg tacgcaaatg atccaactgt
     1921 tggtggtagc
//
LOCUS       DAOPLS010000155         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_70420_length_1918_cov_4.715513, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000155 DAOPLS010000000
VERSION     DAOPLS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70420_length_1918_cov_4.715513"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..521)
                     /locus_tag="PKV52_03475"
     CDS             complement(15..521)
                     /locus_tag="PKV52_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HPD99304.1"
                     /translation="MNDSIEEAIQYAKKYLEDLLSFFGLNTEVHATAEDDVIELSVPS
                     THMNGFLIGRRGDTMRALQYMVSMSLRSKQYAYTRVNVDIADYKKHRQDRIAETALEW
                     INQVKETNEPLSLKPMNAADRRIVHKVAAEYSLETVSEGEGRDRHIIIRPPASTEEPT
                     ADTEALAD"
     gene            complement(524..1531)
                     /locus_tag="PKV52_03480"
     CDS             complement(524..1531)
                     /locus_tag="PKV52_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPD99305.1"
                     /translation="MFQFIGSLFNALVGRPVFNLLIIIIALLPGHNFGVAIIIFTIIV
                     RLALYPLLKKQLHHAMAMRKLQPEIKRIKKESKGDKQKEYALMQELYKEREIKPLASI
                     GILLAQLPILLALFYAISKITQNPETIVSNGYFWVEKLPYIQQLAAGTETLHTVFLGF
                     IDLARPAVDNSVIYWPAMLLVVASVITQYIQSKQLMMTDKNARSLRQILKDSGSGKEV
                     DQTEVQAATSKFTLYIIPFFLFVVAINLASALSLYWFVGALVAIVQQRRILQQDVTEM
                     EASVDGKKVEAEVISKEPAVKVDKKTGVKTYVKQAAPETPKPKKSTKSTKRKAKSKKR
                     R"
     gene            complement(1549..1896)
                     /gene="rnpA"
                     /locus_tag="PKV52_03485"
     CDS             complement(1549..1896)
                     /gene="rnpA"
                     /locus_tag="PKV52_03485"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPD99306.1"
                     /translation="MIAKKHRFYGQGGIRYINRKGSTVRGSLFTVRYVPNKRPASYRA
                     AVVVSKKVTKSAPKRNRIRRRLYELMRTEMGARVLGYDIVIIVFSDKLATMPPQELKA
                     HLSEVLAQVGATS"
BASE COUNT          497 a          421 c          380 g          620 t
ORIGIN      
        1 ataggcttgt cggcctagtc ggcaagtgct tctgtgtctg cggtaggttc ttctgtgcta
       61 gctggtggtc taataataat gtgtctgtcg cggccttcgc cttctgacac cgtttctaac
      121 gaatattcag cggctacttt atgcacaatg cgtctatcgg ccgcattcat aggctttagg
      181 cttagtggtt cgtttgtttc ttttacttgg ttaatccatt ctagagccgt ttctgcaatg
      241 cggtcttgac gatgtttttt ataatctgca atatccacat tcacgcgtgt gtaagcgtac
      301 tgcttgctcc taagtgacat acttaccata tactgcaagg cacgcatggt atcgccccgc
      361 cggcctatta aaaagccgtt catgtgggta ctagggaccg atagttctat aacgtcgtct
      421 tctgccgtgg catggacttc tgtatttaac ccaaaaaaag acaataaatc ttctagatat
      481 tttttagcat attgtatggc ttcttctatt gaatcgttca tggtcacctc ctcttttttg
      541 acttcgcttt gcgcttagta gatttggtag atttttttgg tttaggagtt tctggtgctg
      601 cttgtttaac gtaggtttta acaccagttt ttttatctac cttaacggct ggttctttag
      661 atattacttc tgcttctact tttttaccat ctaccgatgc ttccatttcg gttacatctt
      721 gttgcaaaat acggcgttgc tgaacaatcg ccactaatgc gccaacaaac caatacagcg
      781 ataacgccga tgctaagtta atggccacaa caaacaaaaa gaacggaatg atatacaagg
      841 taaacttact ggtagctgcc tgaacttctg tttggtctac ctctttaccg ctgccactat
      901 cttttagtat ttggcggagc gaacgagcgt ttttgtctgt catcataagc tgtttgctct
      961 gaatatactg agtgataaca ctcgctacca caagtagcat ggctggccag taaataactg
     1021 aattatctac cgccggacgc gccaggtcta taaaacccaa gaatacagtg tgtaatgttt
     1081 ctgtaccggc tgctaactgc tgaatatatg ggagtttttc tacccaaaaa tagccgttac
     1141 tcacaattgt ttctggattt tgtgtaattt tagaaattgc gtaaaacaaa gccagcagta
     1201 tgggtagctg ggccaataaa ataccaatag acgccagcgg cttaatttcg cgttctttat
     1261 acagctcttg catcagagcg tattcttttt gcttatcgcc cttagattct tttttaattc
     1321 gtttaatctc tggctgtaat ttgcgcattg ccatagcatg atgtagctgc tttttaagga
     1381 gcggatacag cgccagccgt acaataattg taaagataat aatagcaaca ccaaagttgt
     1441 ggccaggcag tagcgctata ataataatta atagattaaa tactggcctt ccaacaagtg
     1501 cgttgaataa gctaccaatg aattgaaaca tacttaccta ccagtgtatc acgaagtagc
     1561 gcctacttgc gccaaaactt cagataaatg agcctttagt tcttggggtg gcatcgtagc
     1621 aagcttatca ctaaagacaa taataacaat atcgtaacct aaaactcggg cacccatttc
     1681 ggtacgcata agttcgtaca aacgccttcg tatacgattt ctttttggtg cagacttagt
     1741 tactttttta cttaccacca cagcagcccg atacgacgct ggccgcttat tcggcacgta
     1801 acgcacagta aagagcgatc cacgcacggt agatcctttt ctgtttatat agcgaatacc
     1861 gccttggccg taaaatctat gtttttttgc tatcataaat ggttctactt acttaata
//
LOCUS       DAOPLS010000156         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_70916_length_1909_cov_3.910464, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000156 DAOPLS010000000
VERSION     DAOPLS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70916_length_1909_cov_3.910464"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKV52_03490"
     CDS             complement(<1..240)
                     /locus_tag="PKV52_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99307.1"
                     /translation="MSETYEVPSEDYEKRLAWAEYRVDNLVAKYLAMADREYAKFRDD
                     SWDSDIAGAWFTKEMLDARRKYELYITVAHDIASIM"
     gene            complement(237..824)
                     /locus_tag="PKV52_03495"
     CDS             complement(237..824)
                     /locus_tag="PKV52_03495"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99308.1"
                     /translation="MRTPESIVLVDMDGVMADFDGKVLQDLEDLYPHVPIPHPRNSFY
                     IDDDMAPEHKPIVREIISKPGFFTQLGVIEGTHQGWELLLDAGYTPQICSAPLRKNPT
                     CEQDKLAWLEEHFVPQFGHWVIDTAIITKDKASCSGLAIIEDRPEIKDAHRAEWKHVV
                     FDRTYNQDARSPYRLDGWKDPFIIPLLRTIEQEVL"
     gene            1013..1603
                     /locus_tag="PKV52_03500"
     CDS             1013..1603
                     /locus_tag="PKV52_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99309.1"
                     /translation="MRPPRVESNLYQYHLKQLLFFGYVAKVDGFYTLANKGLAYAARH
                     STTLKKERSQPQVLTILFVINQNGQVLIRIKHRQPFIGMGGLVIGKMHRDETVFAAAK
                     REFFERVCETAESIEFDYFGTAHVVVRQHGCVISDYIGLLIRVAVPNDILLKSKGAFY
                     DINAIDRCRLSPGVDELLDAYARQTTFVEIETEFTT"
     gene            complement(1607..>1909)
                     /locus_tag="PKV52_03505"
     CDS             complement(1607..>1909)
                     /locus_tag="PKV52_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99310.1"
                     /translation="GFTTDTGVVAQAFELHCPIVIKTTKVDGVYDKDPEHHSDAVRYE
                     RIAYSDVLANRHIAVMDKAAMAFAADKAITIGICQPNPQDVLALLGGDTSRGTLVS"
BASE COUNT          517 a          400 c          394 g          598 t
ORIGIN      
        1 cataatagat gcaatatcgt gagcgaccgt tatatatagt tcgtattttc ttcttgcgtc
       61 taacatttct ttagtgaacc atgcgccagc aatgtcagaa tcccaactat catctctgaa
      121 tttcgcatat tctctatctg ccatggctaa gtattttgct actaaattat ctacacggta
      181 ttctgcccac gcaagccttt tttcataatc ttcagaaggc acctcatagg tttcgctcat
      241 agcacctcct gttcaatggt tcgtaatagt gggatgataa acggatcttt ccagccgtct
      301 agtctgtacg gcgatcgtgc atcttggttg taggttcggt caaatacaac gtgcttccat
      361 tctgctctat gggcatcttt aatttctggt cggtcttcga taattgctag cccgctacaa
      421 cttgccttat ctttggtaat gatggctgtg tctataaccc aatgcccaaa ttgtggaacg
      481 aagtgctctt ctagccatgc aagtttatct tgctcgcagg ttgggttttt tctgagtggt
      541 gcgctgcata tttgtggtgt gtagccagcg tctagtagta attcccagcc ttgatgggtt
      601 ccctcaataa cacctaactg cgtaaaaaaa ccaggctttg atataatttc acgcacaatt
      661 ggtttgtgtt ctggcgccat gtcgtcatct atataaaagc tatttcttgg gtgtggtatt
      721 ggcacgtgtg ggtacaaatc ttcgagatcc tgaagaacct ttccatcaaa atccgccatg
      781 acaccatcca tgtctactag aacgatactt tcgggtgtgc gcatattttt aatctccttg
      841 attacagtgt aattctatta caaggataag aacgaatata gaaaaatagt attgactcaa
      901 ataaagttga caataatacc attaataaac tgttattatt atatcaatgc attatattca
      961 attatatata cttgacaaac tatcacaagc taagacactt cgtaataaag atatgcgccc
     1021 accacgtgta gagagtaatt tatatcagta tcacttaaaa cagcttttgt tttttgggta
     1081 tgttgcaaaa gtagatggtt tttatacact tgctaataaa gggcttgcct atgccgcaag
     1141 acacagtaca acattaaaaa aagagcgttc ccagccgcag gtacttacga tattgtttgt
     1201 aataaatcag aatgggcagg tacttattag aattaaacac cgacagccgt ttataggtat
     1261 gggcggcttg gtcatcggta aaatgcatcg agacgaaaca gtatttgccg ctgctaaaag
     1321 agagtttttc gaacgggtgt gtgagacggc tgagtctata gaatttgatt attttggaac
     1381 cgcacatgtt gtcgtgaggc agcatggctg tgtcatttct gactacatcg gactacttat
     1441 acgagttgct gtaccaaacg acatcctcct gaaatccaaa ggtgcttttt acgatataaa
     1501 cgcgatagat agatgtcgtt tatcgcctgg tgttgatgag ctgcttgatg cgtatgctcg
     1561 gcagaccaca tttgtagaaa ttgaaacaga atttacaact tagtggttag ctaactaaag
     1621 tgccacggct cgtgtcgccc cctagcagcg ctaacacatc ttgtgggtta ggttggcaaa
     1681 tgcctattgt gattgccttg tcggcagcaa acgccatggc ggctttgtcc attactgcta
     1741 tatgtctatt tgcgagcaca tcactataag caatacgttc atatcgcacg gcatcagaat
     1801 ggtgctctgg gtctttgtcg tacacaccat ctaccttagt ggtttttata acaatcggac
     1861 aatgcagctc aaatgcttgt gcgaccacgc cagtatctgt cgtaaaacc
//
LOCUS       DAOPLS010000157         1898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_71484_length_1898_cov_4.180141, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000157 DAOPLS010000000
VERSION     DAOPLS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71484_length_1898_cov_4.180141"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..599
                     /gene="rpsE"
                     /locus_tag="PKV52_03510"
     CDS             63..599
                     /gene="rpsE"
                     /locus_tag="PKV52_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPD99311.1"
                     /translation="MNNTATKPRRDTRGPREQEEKQFEERVVSIDRVARVVKGGRRFR
                     FRALVVIGDKKGQVGIGIAKGADVQAAVSKAVDMAKKRLVTVPIFNGTLPHEAQAKVA
                     GANIFVKPASPGTGLIAGGVVRTVLEIAGYSNALSKSLGSSNKINSAYATIAALQSIS
                     PSSEWVVQPVAKKPKAGK"
     gene            609..1073
                     /gene="rplO"
                     /locus_tag="PKV52_03515"
     CDS             609..1073
                     /gene="rplO"
                     /locus_tag="PKV52_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPD99312.1"
                     /translation="MQYHELSVEKRKTAKRVGRGISAGQGKTAGRGTKGQSSRTGGKR
                     RPGFEGGQTPLMMRIPKLRGFTSHRMKAENVYTGQLDTLKGEINNYTLFAAGLVSSPY
                     VKVKLVTKGELTKSVDVHLQAASNSAIAAIEKAGGSFKVVGRPMRESKKTKS"
     gene            1173..1613
                     /locus_tag="PKV52_03520"
     CDS             1173..1613
                     /locus_tag="PKV52_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99313.1"
                     /translation="MQDDTRTLMKPQKTKTRYGILAQSAHSTTCKHRFRKRIYIPSII
                     AALILAGAAGYWLYVLRSTKTIKSWQSAVISLASVDYLEQVYRFGANADGLGPKGTIT
                     MRSDFSDPATPKSKGTFDMDVGVIGRTIKLKSDFLCVSEVECYI"
BASE COUNT          626 a          360 c          432 g          480 t
ORIGIN      
        1 tatcatggac gtattaaagc cctcgcggat gctgtacgtg cagaagggtt ggagttttaa
       61 ttatgaataa tacagcaaca aaaccacgca gagacacacg tggaccaaga gaacaagaag
      121 aaaagcaatt tgaagagcgt gtagtaagta ttgaccgcgt tgctcgagtc gttaaaggcg
      181 gaagacgatt tcgttttcga gcactcgttg tcattggtga taaaaaggga caagtaggca
      241 taggtatagc aaagggagca gatgtacaag cagctgtttc aaaagcggtc gacatggcta
      301 agaaacgact cgttacggtg ccgatattta atggcacatt accacacgaa gcacaggcaa
      361 aggttgctgg cgcaaatata tttgttaagc cagccagccc aggtaccgga cttattgcag
      421 gtggtgtagt acgtactgtc ttagagatag caggctatag caatgctttg tctaagtcgc
      481 ttggatcgtc aaataaaatc aattccgctt atgcaacaat tgccgcctta caatctattt
      541 caccaagcag tgaatgggtt gtccagcctg tggcaaaaaa gccaaaagca ggaaagtagg
      601 agaatactat gcagtatcat gaattatcag tagaaaagcg taaaacagcc aagcgagttg
      661 gacgtggtat ttctgctggg caaggaaaga cagcaggacg gggcacaaaa ggtcagagtt
      721 cacgaacagg cggtaagcga cgaccaggtt ttgaaggtgg tcaaacacct ttaatgatga
      781 gaattccaaa acttcgtggg tttacgagtc atcgcatgaa agccgaaaac gtctatacag
      841 gtcaactcga caccctaaag ggagaaatca ataactatac cttatttgca gctggtctgg
      901 tatcgagtcc atatgtgaaa gtaaagctcg ttactaaagg tgagcttaca aaaagcgtag
      961 acgtgcactt acaagctgct tcgaactcag ctattgcggc aattgaaaaa gccggtggta
     1021 gttttaaagt cgttggtcgg ccgatgcgtg agtctaaaaa gaccaaaagc taaaccatca
     1081 gcgttgcttt atactaaaaa actcccttac acacgtaggg gtgtttttta ataaataatc
     1141 tatagtataa gctcaggtgc tacactacaa taatgcaaga tgatacaaga acccttatga
     1201 aaccccaaaa aacaaaaaca cgatatggaa ttctggcgca atcagcacat agcacaactt
     1261 gcaagcaccg ctttcgtaaa cgaatttata taccaagtat tattgctgcg ttgatactcg
     1321 ctggtgcagc aggctattgg ctatatgtgt tacgatcaac caaaacaatt aagagctggc
     1381 aatcagctgt aatttcgtta gcatctgttg attaccttga gcaagtttat aggtttggcg
     1441 ctaatgcgga tggacttgga ccaaagggta caattactat gcggagtgat tttagtgacc
     1501 ctgccacgcc taaatctaaa ggtacttttg atatggatgt aggcgtaatc ggccgtacca
     1561 taaaactaaa aagtgatttt ttatgtgtat ctgaagtgga gtgttacata taatatattg
     1621 cattcactgc accagaagaa ctcactacag aaaaacctgc atatttaaat acatggctgc
     1681 aatttgatcc tactactaat aacaataact ctgctgatgt atttggtatc gttggtaacg
     1741 ttaatactgt tttaggcgag cttattatag gaaatattac tggtcaagct aagcaaatga
     1801 ttcatgatgc agccaagaat aaaactacat acaaaataat gtctacgcgg gtgagtaaaa
     1861 acagtaatca aaacagtgtg cagtatgaca ttcgcgcc
//
LOCUS       DAOPLS010000158         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_71764_length_1893_cov_2.769859, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000158 DAOPLS010000000
VERSION     DAOPLS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71764_length_1893_cov_2.769859"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKV52_03525"
     CDS             complement(<1..798)
                     /locus_tag="PKV52_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99314.1"
                     /translation="MNKRTSIILASIGLLVMSFMAYTSINHTVFADEPRQFSQPTTNI
                     YDKTIYRVNQRLVMNDLVNGDLYCVADNAIIDAVVNGDVMCLGNTVTLKGTINNNARI
                     IAQNIVLDGTVAGNATLIGSDIRINKDAAIAGDATVVANTLQLSGTVGRDVTIRAKTV
                     NISGTIDRDVYSYHRTMTITDSAQLGTITYSSPQALEVPAGTVSGQINQIATNAPTYL
                     SSLATGTVFLAALLYIAWFISLIITALGVAFLFPKSLEDSVVYAKKQP"
     gene            complement(859..>1893)
                     /locus_tag="PKV52_03530"
     CDS             complement(859..>1893)
                     /locus_tag="PKV52_03530"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPD99315.1"
                     /translation="PYKGGIRFHPDVTRSEVVALSILMSLKTALLDLPLGGGKGGIAV
                     DPKQLTAHELEELSRKYVAYLSPYIGPQQDIPAPDVQTNSQIIDWMTDEYHKITGDTS
                     GASFTGKSLKKGGSNGREAATGRGGFIVAQTIMQSNNITDVSYGLQGLGNVGSFFATE
                     ASTLQPRWQLQAASDSKTAISSKDIDAQSLVAYKKHNQKIAGFPNTVELSSNQLIRGE
                     YTMLVLAALGNVITPANASVVRARFIIELANGPVTDKATDILFQQGTEVVPDILANAG
                     GVVVSYYEWLQNMQKKHWTHEDVNTKLDATMTQATVDVLDYAKLTNTTLKQAAFDVAV
                     CKLLDLPLRK"
BASE COUNT          502 a          400 c          383 g          608 t
ORIGIN      
        1 tggttgtttt tttgcataca caacactgtc ttcaagagat tttgggaaca agaatgcgac
       61 cccaagagcc gtaataatga gcgatataaa ccatgctata tacaacaaag ctgctaagaa
      121 tacggtacca gtagcgagac tacttaaata cgttggtgca ttggtagcta tttgattaat
      181 ttgaccacta acagtacctg ctggtacttc tagtgcctgt ggcgaagaat atgttatcgt
      241 acccaattgt gcagaatcag ttatcgtcat agtcctatgg tatgaataga catcccgatc
      301 gatagtgcca gatatattga ctgtttttgc gcgaatcgtc acatcacgac caacagtacc
      361 acttagctgt agtgtgttgg cgacaacagt tgcatcaccc gctatggcag cgtctttgtt
      421 tatgcgtatg tctgaaccaa ttaaggtagc attgcctgca acagtgccat caagcacaat
      481 gttttgtgca ataatgcgag cattattatt gatagtacct ttaagagtta ccgtattacc
      541 caagcacatc acatcaccat tcactactgc atcaataatt gcgttgtcgg ctacgcaata
      601 aagatcgcca ttcaccaaat cgttcataac taaacgttga tttacgcgat atattgtctt
      661 gtcataaata tttgtagtag gctgagaaaa ctgtcttggt tcgtctgcaa aaactgtgtg
      721 gtttatagat gtataggcca taaaactcat gactaacaac cctatacttg caagtattat
      781 tgaagtacgc ttattcatac atattaaact ccctactatg tgtagtatac caccatattt
      841 gaggtgtttt attagtcttt atttacgtag cggtagatct agaagcttac atacggctac
      901 gtcaaacgct gcttgcttaa gtgttgtgtt ggtgagcttc gcataatcta agacatctac
      961 tgtggcttgt gtcatcgtag cgtctagctt tgtattaaca tcttcatggg tccaatgttt
     1021 cttttgcata ttttgtaacc actcatagta gcttactacc acccctccag cgtttgcgag
     1081 tatatcaggt acgacttcag ttccctgttg gaaaagtata tctgtagctt tatctgtaac
     1141 gggaccattg gctaattcta tgataaaccg agctctaaca actgatgcgt tggctggggt
     1201 gataacgttt cctaaagccg ccaaaacaag catcgtgtac tctccacgta ttagctggtt
     1261 actgctaagt tctacagtat ttggaaaacc tgctatcttt tggttgtgtt ttttgtatgc
     1321 aactaaggat tgtgcatcaa tatctttgct agaaatcgct gtttttgagt cagatgcagc
     1381 ttgtagttgc cagcgaggtt gcagggtgct tgcttctgtg gcaaaaaaac ttccgacatt
     1441 gccgagacct tgcagaccat agctcacatc tgtaatatta ttagattgca taattgtttg
     1501 agccacaata aacccgcctc taccggtagc agcttctctg ccattgctcc cacccttttt
     1561 tagtgacttt cccgtaaaac tagctcctga agtgtcacca gttattttgt gatattcatc
     1621 tgtcatccag tcaataattt gtgaattagt ttgtacatct ggtgcaggta tgtcttgttg
     1681 cgggccaata tacgggctaa gatacgctac gtattttcgg gatagttctt ctagttcatg
     1741 tgcggtaagt tgttttgggt ccacagctat gccgccctta ccacccccga gcggtaagtc
     1801 taataatgct gtcttaagac tcattaagat actaagtgct actacttcgc tgcgggtaac
     1861 gtctgggtga aaacgtattc cacctttata agg
//
LOCUS       DAOPLS010000159         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_72028_length_1888_cov_3.361702, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000159 DAOPLS010000000
VERSION     DAOPLS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72028_length_1888_cov_3.361702"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PKV52_03535"
     CDS             complement(<1..1358)
                     /locus_tag="PKV52_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD99316.1"
                     /translation="MAAFGHYERGKIIIATDIAKTSITIDDIDVVIDCGWERGSDTRQ
                     DVPLLDIRPACIATKDQRKGRVGRVKPGIYVQAPLPGYPELPPASELSDYDTPEILKN
                     RLDSLMLRTLGNAAFTLDELDLICEPEEQLKQYAKQRLQRLGAVAVTGDVTQTGIEMA
                     KLPLDAHYARMVVESLKYDKELQVMMLAAVAAHANTTITHTSADANLTYGKLTDEQNS
                     EILMMLDVFIAAQTMNQDELETFGIVEKKYLNAKKSFESLLSRLNLEDVALTAPTVEQ
                     EEQLLRCIISGADELYVPINRFKHLDPRGYMRNISKNSPLRGSKVTVVGNPFDIANVS
                     SNGKSYKSLIKGATRVTTQQLLDVAPHRCTFEPAEYVLRYDGVVLAKKEVYFDDLTTR
                     DFITEPAEPSSDLIESVVLSILYGKLPNLNLPRCESLRQTIEAVHDLEARTTNNLDLT
                     TL"
     gene            complement(1431..>1888)
                     /locus_tag="PKV52_03540"
     CDS             complement(1431..>1888)
                     /locus_tag="PKV52_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99317.1"
                     /translation="LSWKIDGLPDDYVGYRTANEGDSTHESVAEIVTDGLEVRRLLSS
                     GGLGQDTILIVGEVHEANANITMLLALIKRMGIRAVIMSATFNTEGTAEYLCDEQGNP
                     APIIDIEGRTYEVEERQGESLVDEAVRYAKEEKNILIFVPGKPDITRVT"
BASE COUNT          479 a          423 c          383 g          603 t
ORIGIN      
        1 aatagagtgg ttaggtctaa attattagtc gttcttgctt caagatcatg gacggcttct
       61 atcgtctgac gcaatgactc gcagcgcggt agatttaggt ttggtagctt gccatataat
      121 atagacaaca cgaccgattc aataaggtca ctacttggct cagctggctc tgtaataaaa
      181 tctcttgtag ttaaatcatc aaagtacact tcttttttgg ccagcaccac gccatcgtat
      241 cgaagcacgt attcggctgg ttcaaaagta caacggtggg gggctacatc tagtaattgc
      301 tgagtcgtga cacgggtagc tcctttaata agtgatttat agcttttgcc gttactacta
      361 acattagcaa tatcaaaagg gttcccgaca acagttactt tactaccgcg gagcgggcta
      421 ttcttactga tattacgcat ataaccccgt ggatctaggt gtttaaaccg atttatcggc
      481 acatatagtt catcggcacc actaataata caacgtagta actgctcttc ttgctctact
      541 gtcggcgctg ttaatgctac atcttctaga tttaatcgtg ataataaaga ttcaaatgat
      601 ttcttcgcat taaggtactt tttttctacg ataccaaacg tttctaattc atcttggttc
      661 atggtttgtg cagcaataaa gacatcaagc atcattaaaa tttctgagtt ctgttcgtct
      721 gttaatttgc catatgtaag gttagcatcg gcagaagtat gggtgatagt agtgttcgcg
      781 tgtgctgcaa cggcagctaa catcataacc tgcaactctt tgtcatactt aagagattca
      841 acaaccatcc gagcataatg agcatcaagc ggtagctttg ccatttctat accagtttgc
      901 gtcacgtctc cagttaccgc caccgcaccc aatcgctgca agcgttgttt tgcatactgc
      961 tttaattgtt cttctggctc gcatataaga tcgagctcat ctaaagtaaa agcagcgttg
     1021 ccaagtgtac ggagcattaa cgaatctaac ctatttttaa gtatttctgg tgtatcgtaa
     1081 tctgatagtt cactggctgg tggtagttct gggtaaccag ggagcggtgc ttgtacatat
     1141 atacctggtt ttactcgccc aacacgacct ttccgttgat ccttcgtagc tatacaggca
     1201 ggacggatat cgagcaacgg tacgtcttgg cgggtatcgc tacctcgttc ccaaccgcag
     1261 tctataacaa catcaatatc atcaatagtg atactagttt ttgctatatc tgtagctatg
     1321 ataattttgc ccctttcgta gtgcccaaat gcagccattt gttctgttgc agattgttcg
     1381 ccatggagcg gtataattac gatatcttgt ttaagtttcg tacgtattag tcacgtaact
     1441 cgagtaatat caggtttacc tggtacaaaa atgaggatgt ttttttcttc ttttgcgtaa
     1501 cgtacggcct catctactaa tgactcacct tggcgttctt ctacttcata tgttctacct
     1561 tcaatatcga tgataggtgc aggatttcct tgctcgtcgc aaaggtattc ggcggtgcct
     1621 tctgtattaa atgtagcgct cataatgact gctcgtatac ccatacgttt tatgagtgcc
     1681 aagagcattg taatgttagc atttgcctcg tgtacttcgc ctacaataag aatcgtgtcc
     1741 tgtcccagcc cgccgctact taataaccgc cgtacttcga ggccgtctgt gacaatttct
     1801 gctacacttt catgcgtgct atcgccttcg ttggctgtac ggtaaccaac gtaatcgtca
     1861 ggcagaccat caatcttcca agataatt
//
LOCUS       DAOPLS010000160         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_72444_length_1881_cov_3.599124, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000160 DAOPLS010000000
VERSION     DAOPLS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72444_length_1881_cov_3.599124"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKV52_03545"
     CDS             <1..336
                     /locus_tag="PKV52_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99318.1"
                     /translation="QAQQLANQQVEYIRASDAGSNGGSTRNDFASGSFTCLKNVAGKL
                     EVQADCKDISMGGANIYAIEISCVDQATKAIGAICSTNKTTNSVYSIVVTWANVKGTQ
                     SKLELQYAM"
     gene            326..904
                     /locus_tag="PKV52_03550"
     CDS             326..904
                     /locus_tag="PKV52_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD99319.1"
                     /translation="MPCSKKGFTIVELMIAMLVFGVASLLVTAAVIGMSRQYQKGSYT
                     AQLNDASRSIHQDIFNAVAYVNKDAVTNMKTEDSVTYFCIGNVMYYWSLPDGNTINNG
                     LFKRQLSDAACERGQANGGTNLLPRNGFVSQFTITQPSADLGVFDITTRFNVGTYDMY
                     KNNSYTECLPTLRGGDFCTIVNYNSSVKPRIQ"
     gene            905..>1881
                     /locus_tag="PKV52_03555"
     CDS             905..>1881
                     /locus_tag="PKV52_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99320.1"
                     /translation="MGYIKMQMNKKPINFNQSGASAIMFALFFIMIISLISVGFATLS
                     RRDQRATIDKTLSSQAQLAAESGINAANTYISEKVKSGSLGASNPGSVKNGCEEVGNF
                     KYPVFSEGVRITCVTWDAKPEEAVKTLSPYDGWSFTTTNFTGKDLISWKVDAFADSMS
                     AYDGAIGRTNLKPVQPGKLPILKVASVSTADITNTAQPKIEVYYLIPASKAGENIAPT
                     PINLEDGPTNNRGNAAAFNVTCSANGECKADLNGYANASSLPNSRLYYFQIIGDQSAT
                     LKYQALSGAVPVKLDNIQTKIDVNAIAQDQSKRLISYISTSPGSNSTWQP"
BASE COUNT          623 a          332 c          398 g          528 t
ORIGIN      
        1 caagcgcaac aactggcaaa tcaacaggta gaatacatac gtgcttctga cgctggctct
       61 aacggtggtt cgactagaaa tgattttgca agtgggtcat ttacttgtct taaaaatgtc
      121 gcgggtaagc tagaggtcca agcagattgt aaagatatta gcatgggtgg tgcaaatata
      181 tatgcaattg aaatatcttg tgtagatcag gcaacaaaag ctataggtgc aatttgtagt
      241 acgaacaaaa caacgaattc tgtatactct attgttgtta catgggctaa tgtcaaaggt
      301 acgcagtcta aactggagtt acaatatgcc atgtagtaaa aaaggtttta caatagttga
      361 gcttatgata gctatgctag tatttggcgt tgcgtcatta ctggttactg cagccgtaat
      421 aggtatgtca cgtcaatatc agaaaggttc atatacagca caattaaacg atgcgtcgcg
      481 tagtattcac caagatatat ttaatgccgt tgcatatgtt aataaagatg cagtgaccaa
      541 catgaaaaca gaagattctg taacatattt ttgtattgga aatgtaatgt attattggtc
      601 attaccagat ggtaatacca taaataatgg attgtttaaa aggcaactaa gcgatgcagc
      661 ctgcgaacgt ggccaagcaa atggtggtac aaatttacta ccgcgcaatg gatttgtcag
      721 tcaatttacc attacacaac catcagcaga tcttggtgta tttgatataa ctactcgttt
      781 taacgtaggc acatatgata tgtataaaaa taattcgtat accgaatgct tacctacatt
      841 gcgtggcggt gatttttgta caatagtaaa ttataatagt agtgtgaaac cgaggataca
      901 ataaatggga tatataaaaa tgcaaatgaa taaaaagcct attaatttta atcaatctgg
      961 ggcatcagca ataatgtttg ctttgttctt tattatgata atatcgctta tatcggttgg
     1021 ttttgcaacg ctgtcgagac gcgatcaacg ggcgacaata gataaaacac tatcatcaca
     1081 agctcaactg gcagctgagt ctggtataaa tgcagctaac acatacatat cggaaaaagt
     1141 gaaaagcggt agtttgggtg cttctaaccc aggtagtgta aaaaacgggt gtgaagaagt
     1201 tggtaacttt aaatatccag ttttttctga aggtgtacgc atcacttgcg tgacatggga
     1261 tgccaaacct gaagaagcag ttaagacact gtcgccgtat gacgggtgga gttttaccac
     1321 aacaaatttt acaggtaaag accttatttc ttggaaggtg gacgcatttg ccgacagtat
     1381 gagtgcatat gatggggcca ttggtcgtac gaatttgaaa cctgtgcaac caggcaaatt
     1441 acccatactg aaagttgcta gtgtatcaac tgcagatata accaatacag cacaaccaaa
     1501 aatagaagtt tattacctta taccagcttc taaggcgggt gaaaatattg ctccaacgcc
     1561 aataaattta gaggatggac ctactaacaa taggggtaat gcggctgcat ttaacgtgac
     1621 ttgctcagcc aacggtgaat gtaaagcaga tctgaatggc tacgcaaatg cttcttcgtt
     1681 gcctaattct aggttatatt attttcagat tataggtgat caatccgcta cactgaagta
     1741 ccaagcattg agcggtgccg tacctgtcaa actagacaac atacagacaa aaattgacgt
     1801 aaatgctatc gcgcaagacc aatctaaaag actaattagc tatatctcaa catctccagg
     1861 gtcaaatagc acgtggcaac c
//
LOCUS       DAOPLS010000161         1877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_72636_length_1877_cov_4.276070, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000161 DAOPLS010000000
VERSION     DAOPLS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72636_length_1877_cov_4.276070"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PKV52_03560"
     CDS             <1..894
                     /locus_tag="PKV52_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPD99321.1"
                     /translation="RTINNSPFISLIHPWECGLDNTPYWLKIMRTYTPLKLKILQFLH
                     LEKLLNKTRKDTKQVPASERPEPIDLYIFYNIIRQAKQNGYNARRISNTKAPVIEDLV
                     FNAIFIRANDLLMTIAAEIDYSIDADLVAHCKNTKAKIDQLYEQGTFWSRDVTTGKLI
                     KEQTIANFIMLYSGALSREQAQAMMNDLHGDGIWRPAYGVTTTPTNSRWFLPKNYWRG
                     PVWVNMNWLIIDGLRRYGYHKEAEVMRNQTIAMVANGGVCAEYYSPIDGTPAGSPQFS
                     WTAALTIDLIKTTKLKIATNE"
     gene            complement(884..1441)
                     /locus_tag="PKV52_03565"
     CDS             complement(884..1441)
                     /locus_tag="PKV52_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPD99322.1"
                     /translation="MQNTDHIKTYKQSKGYLHSQLLGLHVTFIAVYIAIIALSIEGDT
                     VFNIAGLIVGEFDISIILVGFIVQMLFSFVVMNHWSNTMFKITDNAVAITTGIISQHT
                     QTISLDDIVAPQVHQSPLGTYYSYGTITFNVRFSDEPIQLRGVSMPNTFIEDMRIQMR
                     NSAPYYNMEQSLPLAYIRTHQQTIH"
     gene            complement(1615..>1877)
                     /locus_tag="PKV52_03570"
     CDS             complement(1615..>1877)
                     /locus_tag="PKV52_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009523 - photosystem II [Evidence IEA];
                     GO_component: GO:0009654 - photosystem II oxygen evolving
                     complex [Evidence IEA];
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HPD99323.1"
                     /translation="TAISDSIKKSNPETTTTYESRQIKNGLDTLTLVTTYKGETEKVK
                     AKTTIFLKKDGSVYTISTQSPEANWDKYQDSFDEIHNTFQYN"
BASE COUNT          604 a          330 c          366 g          577 t
ORIGIN      
        1 agaaccatca ataattcgcc gtttataagc ctaatacacc catgggaatg cgggcttgat
       61 aacacaccgt attggctaaa aataatgaga acttatactc cattaaaact aaagatattg
      121 cagtttttac atttagaaaa gttgctaaat aagacaagaa aagataccaa acaggttcct
      181 gcaagcgaac ggccagaacc tatagattta tacatttttt ataatattat taggcaagca
      241 aaacaaaatg gatataatgc gcggcgtata tcaaatacca aagcccctgt catagaagat
      301 ctagtattta atgcaatttt tatacgagcg aacgatttgt taatgactat cgcggcagaa
      361 attgactatt ctattgatgc agatttggtg gcacattgta aaaacaccaa agcaaaaata
      421 gaccagttat acgaacaagg caccttttgg agtagggacg taacaaccgg aaagcttatt
      481 aaagaacaaa ctattgcaaa ttttattatg ctatattctg gggcactatc aagagaacaa
      541 gcccaagcca tgatgaatga tttacacggg gatggtatat ggcggcctgc atatggggta
      601 actaccacgc caactaattc tcgttggttt ttacctaaaa attattggcg tgggccagtg
      661 tgggttaata tgaattggct gattatagat gggctacgcc gttatggtta tcataaagaa
      721 gcagaagtaa tgcgaaacca aacgatagca atggtagcaa atggtggagt ctgcgcagaa
      781 tattattctc ctatagacgg cacacctgca ggatcacctc aattctcgtg gacggcggca
      841 cttactatag acttaataaa aacaacaaaa ctaaaaatag ctactaatga atagtttgtt
      901 ggtgcgtgcg tatgtacgct aaaggtaaag attgttccat attataatat ggcgcactgt
      961 tccgcatttg aatacgcata tcttcaataa atgtgttggg cattgaaact ccacgtaatt
     1021 gtattggttc gtctgaaaag cgtacgttaa acgttatagt accgtaacta tagtatgtac
     1081 caagcgggct ttgatgtacc tgcggagcaa caatatcatc tagagaaatg gtttgcgtgt
     1141 gctgactaat aattcctgtt gtgatagcaa ctgcattgtc tgtaatttta aacattgtgt
     1201 tagaccaatg attcatgaca acgaacgaaa ataacatttg tacaataaaa cccaccagaa
     1261 taattgaaat atcaaactca ccaacaataa gcccagcaat attaaaaacc gtgtctcctt
     1321 ctatgcttaa ggctataata gctatataaa cagcaataaa ggttacgtgt agtccaagta
     1381 attgtgagtg taaatacccc ttactttgtt tgtatgtttt aatgtgatct gtattttgca
     1441 tagtacctcc atatatagtg tgattctata tagcgtatgc cataaacctt atgttttgct
     1501 gtgtatttta gcaaagcgct acaagacgta cggttctata gcaaatacac accacacgcg
     1561 gaattattta ggtaatacca caaggcacga aatggttttt tggctaaggg ttagctagtt
     1621 atactgaaac gtgttatgaa tctcgtcaaa actgtcctgg tatttatccc agtttgcttc
     1681 tgggctctgg gtagatatcg tgtaaacaga gccatctttc tttagaaata tggtagtttt
     1741 tgctttaact ttttctgttt cgcctttata ggttgttact agcgttaacg tatcaagccc
     1801 attttttatc tgtctagatt cgtatgtagt cgttgtttct gggttagatt tttttatact
     1861 atctgatatg gctgtta
//
LOCUS       DAOPLS010000162         1870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_73026_length_1870_cov_3.260055, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000162 DAOPLS010000000
VERSION     DAOPLS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73026_length_1870_cov_3.260055"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKV52_03575"
     CDS             <1..409
                     /locus_tag="PKV52_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HPD99324.1"
                     /translation="ILTLYPQAKVIGEPSSTTTFENAANVAGLIDELGFSTRSLRLVV
                     VASWGHAHRGSLLLQKALPKGPLVEVLPTDVPVSPKERIMEAVLLGLSVAALAGVTPG
                     DTEQFKQAARRYQHLVETPKNSTFIQKIHPGRS"
     gene            complement(452..1534)
                     /gene="ychF"
                     /locus_tag="PKV52_03580"
     CDS             complement(452..1534)
                     /gene="ychF"
                     /locus_tag="PKV52_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPD99325.1"
                     /translation="MSVSIGIVGLPNVGKSTLFNALTNNNILAANYPFATIEPNTGIV
                     PIPDPRLDALAKLYNSRKIIPATVKFTDIAGLVAGASKGEGLGNQFLSHIRECAAIVQ
                     VVRGFTDPNVVHVDNTHNPANDIDVINTELVLADLQTLDKVLPRLQKEARTNPKLKPQ
                     LDILKEVQAILDAGKPLYSDEGLRLEVIGYRELQLLTAKPIIYLFNVDEDSLSNAAVQ
                     TKLAQMVLPNAALFVNAKLEDELRSLDEAERTEMLAEYGVQESGLVQLIHAAYETLGL
                     QSYLTAGEKEVRAWTIKKGATAPQAAGVIHGDFERGFIAADVVDYQDLIAAGSLAAAR
                     AVGKVRTEGKTYVMQPGDVVEFKFNV"
     gene            complement(1531..>1870)
                     /locus_tag="PKV52_03585"
     CDS             complement(1531..>1870)
                     /locus_tag="PKV52_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HPD99326.1"
                     /translation="ETGGALGVREPGLLLSIAEKPKTRFGTYDLYPDIYVKAASLYES
                     ICNYHVFIDGNKRTAVLTMYRFLYLNGCILLADNKTIEEYTLYIVNNKPDITEIAKWI
                     KKHSRKAKTI"
BASE COUNT          483 a          430 c          390 g          567 t
ORIGIN      
        1 tattcttact ttgtacccac aggcgaaggt aataggcgaa ccatcgtcta caaccacgtt
       61 tgaaaacgct gctaatgtcg cagggctcat tgatgagctt ggcttctcta cgcgtagtct
      121 ccgcttagta gttgtggcga gctggggcca cgcccatagg ggtagtctgt tacttcaaaa
      181 agcattgcca aaagggccct tagtagaggt gttgccaaca gatgtgcctg tatcgcccaa
      241 ggaacggatt atggaagcgg tacttttggg tttatctgtg gctgctcttg caggtgtgac
      301 accgggcgac acggaacaat ttaaacaggc tgcaagacgc taccagcatt tggtagaaac
      361 tcccaaaaac agtacattca tacaaaagat acaccccgga cgttcctagt ggtgtttgtt
      421 ggtacacaac tggcctgtaa cacttacact gctagacgtt aaacttaaat tctacgacgt
      481 caccaggctg cataacgtaa gttttgccct ctgtacgcac tttaccaaca gcgcgggcag
      541 ctgccagtga cccggctgca attaaatctt ggtagtctac aacatcagcg gcaataaagc
      601 cgcgttcaaa atcaccatgt attaccccgg cagcttgcgg cgcagtagcg ccttttttta
      661 ttgtccaggc tcggacttct ttttcgcctg cggttaagta gctttgtaaa cccaatgttt
      721 cataagcagc gtgtataagc tgaacaagcc cagattcttg tacaccatat tctgccagca
      781 tctctgttct ttcggcctcg tctaggctcc gtagttcatc ttctagcttg gcattaacaa
      841 acaaggcagc gtttggtaaa accatttgtg caagttttgt ttgcacagct gcattactaa
      901 ggctgtcttc atctacattg aacaaataaa ttataggttt tgccgtaagt agttgcagct
      961 ctctataacc tataacctct aaccttaaac cttcatcgct atatagtggt ttgccagcgt
     1021 caagaatagc ttggacttct tttaaaatat cgagctgcgg tttcaatttt gggttagtgc
     1081 gagcttcttt ttgtaatcgg ggcaatactt tatctagtgt ttgcaaatct gccagtacaa
     1141 gctcggtgtt tatgacgtct atgtcgttgg ctgggttatg ggtattgtct acatgaacaa
     1201 catttgggtc agtaaagcca cgtaccacct gcacaatcgc tgcacattca cggatatggc
     1261 ttaaaaactg gttgcccaaa ccttcgccct tgctggctcc ggcaactaat ccggcgatat
     1321 cagtaaattt tacggtagca gggataatct ttctactatt atacagcttg gcaagtgcat
     1381 caagccgcgg gtcaggaatg ggtacaatac cagtgtttgg ttctattgtg gcaaacgggt
     1441 agttagccgc caatatattg ttgttagtta acgcattaaa cagggtactt ttaccaacat
     1501 ttggcaaccc cacaataccg atacttacac tcatatagtt tttgcctttc tgctatgctt
     1561 ttttatccat ttagcaattt ctgtgatgtc tggcttattg tttactatat aaagagtgta
     1621 ttcttctata gtcttattat ctgcaagcaa tatacagcca tttaaatata aaaaccgata
     1681 catagttagt accgccgttc ttttattacc gtctataaat acatggtaat tacaaatgct
     1741 ttcatatagc gaggcagctt tcacgtatat atctgggtat aaatcgtacg taccaaaacg
     1801 agtctttggt ttttctgcta tcgatagcaa cagcccaggc tctcttaccc ctaaagcacc
     1861 gcctgtttca
//
LOCUS       DAOPLS010000163         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_75214_length_1832_cov_3.494091, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000163 DAOPLS010000000
VERSION     DAOPLS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75214_length_1832_cov_3.494091"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKV52_03590"
     CDS             complement(<1..356)
                     /locus_tag="PKV52_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPD99327.1"
                     /translation="MADILPKSLSALIDGFSALPGVGRRTAERYAYFTLRQDVSISSK
                     LADALTRVHSGIKVCPITFALIDADKEVSALYSDAKRDKTTVAVVAEPFDIIAIENTN
                     QFDGTYHVLGGLISPL"
     gene            complement(372..665)
                     /locus_tag="PKV52_03595"
     CDS             complement(372..665)
                     /locus_tag="PKV52_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPD99328.1"
                     /translation="MGKLDQAKTIMQAKKLQKELQKLIIVGEAGDGAVIVEVNGEQKL
                     KKVRINPEYVDVDDIGQLERWIEDAIKSAMQESQKIAAEKVQPLLGNLGNLGF"
     gene            complement(683..>1832)
                     /locus_tag="PKV52_03600"
     CDS             complement(683..>1832)
                     /locus_tag="PKV52_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99329.1"
                     /translation="IIIGCLATITLGLQINTLIDGQNKFETITLEHLQNFPNPTERMI
                     NAPYLVPAYIVGTIADNMLLGARATSVIFGLLATVVLFMLLKRWFTTQIASVGSLLFV
                     TSSWVLAVSHQATPLILLIITPLLLLVSLTKYVNAKQHVYASFLLLVASLALAAYVPF
                     MFWPIIVVLGAMSYLYRHKLQQLSKKQILIAAGLYALLLVPMLISVTQYPGQLKELIG
                     IPTMILPDVTTYAKNFLWQFSTLFFVAQPFPELYVGSLALLDIFSVAMVFLGIYHFVK
                     YMPKRRKVSFAFLVIVLAFVVPLSTIYQIPMTAYIPIIYILIASGVYELLRQWFSYFP
                     RNPIARNSAVVIVAILIGLATLYNLQRFYIAWPNAPETKAVYMVQSNK"
BASE COUNT          552 a          377 c          345 g          558 t
ORIGIN      
        1 tctagcggcg atatcagacc accaagtaca tgataggtac cgtcaaactg attcgtattt
       61 tctatagcaa taatatcaaa tggttcagcc accacagcca cggttgtttt atctcttttt
      121 gcatcgctat atagcgccga cacttcttta tcggcgtcta taagcgcgaa ggtaattgga
      181 cacactttaa taccactatg cacccgtgtt aatgcatcgg cgagcttgct actgatagat
      241 acatcttggc gcaatgtaaa atacgcgtat cgctctgctg tccgtctccc tacaccaggg
      301 agtgcagaga atccatcaat aagtgcactt agactttttg ggagtatatc cgccatgttt
      361 ttaacgactt tttagaaccc tagattgcca aggttcccaa gtaatggttg caccttttct
      421 gctgcaattt tttggctttc ttgcatcgca gatttaatag cgtcctctat ccaacgttca
      481 agttggccaa tgtcatcaac atctacgtat tctggattta ttcgtacttt ttttagtttt
      541 tgctcaccat taacttcaac aatgacagct ccgtcacctg cttcaccaac aataattaat
      601 ttttgcaatt ctttttgcaa ttttttggct tgcataatcg tctttgcctg atctaattta
      661 cccattgctc actcctcatt tgttacttat tcgattgtac catgtagaca gcttttgtct
      721 caggggcatt cggccatgca atgtaaaatc tttgtaaatt atatagtgta gctaacccta
      781 taagtattgc tactatgacg actgcgctat tacgagcaat aggattacgt gggaaatagc
      841 taaaccactg tcttagtaat tcataaacac cactagcaat gagaatataa ataattggta
      901 tgtaggctgt cataggtatc tggtatatcg tagataatgg tactacaaat gcgagtacaa
      961 tcactaaaaa ggcaaacgaa actttgcgac gctttggcat atatttaaca aaatgatata
     1021 taccaagaaa caccatggct acagaaaata tatcaagcag tgccaatgag ccaacgtaca
     1081 actctggaaa tggctgagcc acaaaaaata gtgtgctaaa ttgccacaaa aagttttttg
     1141 cataggtagt gacatctggt agtatcattg ttgggattcc tatgagttct tttaactggc
     1201 cagggtactg cgtaacgcta atgagcattg gcacaagtaa caatgcatac aacccggctg
     1261 ctataagaat ttgttttttt gataattgtt gtagcttgtg tcgatacaaa taactcatag
     1321 cacccagcac cacaatgatt ggccagaaca taaacggtac ataggctgca agtgcgagac
     1381 tcgcaacaag gagcaaaaaa gaagcatata catgctgttt agcgttcacg tattttgtca
     1441 gacttaccag cagtaaaagt ggtgtaataa tcaataagat tagtggtgtt gcttggtggc
     1501 taacagcaag tacccagcta ctagtgacaa acaacaaact tcctacagat gcaatttgtg
     1561 tagtaaacca ccgttttaac aacataaata gtacaaccgt agctaataat ccaaatatta
     1621 ctgatgtcgc ccgagcaccg agtagcatat tatcggcaat tgtgcctaca atatatgccg
     1681 gcactaaata tggtgcatta atcatacgct ctgttgggtt aggaaaattt tgaaggtgct
     1741 ctagtgtaat tgtttcgaat ttattttggc catcaatcaa agtgttaatt tgtagcccaa
     1801 gtgttatggt cgccaaacag ccaatgatta ta
//
LOCUS       DAOPLS010000164         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_75969_length_1820_cov_2.356374, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000164 DAOPLS010000000
VERSION     DAOPLS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75969_length_1820_cov_2.356374"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..465)
                     /locus_tag="PKV52_03605"
     CDS             complement(202..465)
                     /locus_tag="PKV52_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99330.1"
                     /translation="MRKYKQVPTTRDVVTSLTCDVCLREYDDPTEMQEFLLIEKACGY
                     GSVFGDDVMFEFDICQHCFHNFFAELVHLVEDFADWGNFSLDT"
     gene            complement(462..1241)
                     /locus_tag="PKV52_03610"
     CDS             complement(462..1241)
                     /locus_tag="PKV52_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99331.1"
                     /translation="MAVTHPDHPAPLNTGNFCNALLWNCKGDDCDYHYENATATECPV
                     CGTPRRRCKRPPINGRDACGVHGGKSLIGMAHPSYNGIGLSKHVPTRLLDSLQESLNN
                     PDILNGTGSIALLEARRNELLRRIDSNIPPTEGWFKLSDLLKKYRQAEIRMRSANSET
                     QKRRYFDEGRKLLDEIEDLVIQAYYGDLTWKEINNLEETIRRLRDSEIRRAKAAEELI
                     TPDQFRALVTHIIVIGREEIHDRNDLHKFEDRLQAMIMSLK"
     gene            complement(1430..>1820)
                     /locus_tag="PKV52_03615"
     CDS             complement(1430..>1820)
                     /locus_tag="PKV52_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99332.1"
                     /translation="LTESGFSPRSINAPATDSYIVPTFKIAVKGMKSIPTVAVAMKSQ
                     SNALKQVMYDTTVAYQSAAPDVIRAELQNVRKELRALRTKIQTVKFVVLMAKKWFREF
                     DKYDAQLELEDGDYTYLFEFGEKEIQI"
BASE COUNT          492 a          383 c          352 g          593 t
ORIGIN      
        1 attatcatta ctataaaaac catccccttt gaaaattaca gcaacgggtg caacaatgac
       61 tttccgcatt atccccatac atttttcgca tctgattata cgggtagcgg cggtcatagg
      121 ttccacaacc ggcacagtaa tatcacattt gtcacatttg tatgtatatg ttgccatgta
      181 tggtattata cacctatttt gttaggtgtc aagactaaaa ttgccccaat cggcaaaatc
      241 ctcaacaagg tgtaccaatt cggcgaagaa attatgaaaa cagtgttggc aaatatcaaa
      301 ctcaaacatt acatcatcac caaaaacact accataacca cacgcttttt ctatcaatag
      361 gaattcctgc atttctgtag gatcatcgta ttcgcgtaaa caaacatcac aagttaaact
      421 tgtaacgaca tcacgggttg tcggaacttg cttatatttt ctcatttcaa actcataatc
      481 atggcttgta gccgatcctc aaacttatgt aaatcatttc tatcgtgtat ttcctcgcgc
      541 ccaattacaa tgatatgtgt aacaagtgcc ctgaattggt caggggtgat aagttcttct
      601 gctgcctttg cacggcgtat ttcactatca cgcaaacggc gtattgtttc ttcaaggttg
      661 ttgatttctt tccatgtaag gtcgccataa taagcctgta ttactaaatc ttcaatttca
      721 tcaagtagtt tgcggccttc atcaaaataa cgtcgctttt gtgtttcgct attggcactg
      781 cgcatacgta tttcagcttg gcgatatttt ttaagcaagt cgcttaactt aaaccaacct
      841 tctgttggcg gtatgttgct atcaatgcgg cgtaatagtt cattgcgccg cgcttctagt
      901 agtgcaatac taccagtgcc gtttaggatg tctgggttat ttaacgattc ttgtaaggaa
      961 tccaaaaggc gcgtagggac gtgtttagat agcccaatcc cgttataact tgggtgcgcc
     1021 atgccgatta gtgatttacc accatgtaca ccgcaagcgt cgcgcccatt aataggtggg
     1081 cgtttacagc ggcgacgagg tgtaccacat acaggacatt cggttgcggt tgcattctca
     1141 taatgataat cgcaatcatc acccttgcag ttccaaagca aagcgttaca gaaattacct
     1201 gtatttagtg gtgcgggatg atcgggatgt gttacagcca tagttttagt ttaacaggct
     1261 ggtgtaactg ttaaactcta attatggtac tgtatttgat catttttgta attttatatg
     1321 cactaacggg ttggtggtgg ttgttgtatg tgggctttgt gacgtatggc ttactacgtg
     1381 tccctaccat acgtcacaaa gttttggcta aattactaga ttggttgttt tagatttgaa
     1441 tttctttttc gccaaactca aacaagtatg tgtaatcacc atcttctaat tctaattgtg
     1501 catcatactt gtcaaattca cggaaccatt ttttagccat aagcacaaca aactttacag
     1561 tttgaatttt agtgcgcaat gcccgtaatt ctttacgcac attttgcagt tctgcccgaa
     1621 ttacatcagg ggccgcactt tgataagcca cagtagtatc atacattacc tgtttcaacg
     1681 cattactttg tgacttcata gctacagcaa cggtaggaat ggatttcata cccttaactg
     1741 caattttgaa tgttggtaca atgtaactat ctgttgctgg tgcattgata ctgcgtggtg
     1801 agaaaccact ttctgttagt
//
LOCUS       DAOPLS010000165         1809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_76691_length_1809_cov_3.558153, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000165 DAOPLS010000000
VERSION     DAOPLS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76691_length_1809_cov_3.558153"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PKV52_03620"
     CDS             <1..264
                     /locus_tag="PKV52_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99333.1"
                     /translation="LVYMSQEVLRQSDDDLPINVDELLIEIQLAAKELGNGRKHMDER
                     RKIAYRYIIKENIIALGLDPQNYYGDYRALLQARPSSQIVDEN"
     gene            777..1097
                     /locus_tag="PKV52_03625"
     CDS             777..1097
                     /locus_tag="PKV52_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HPD99334.1"
                     /translation="MDYKTMQQRALQISNLYDMLNKKHAQKIWNEQDVMAGFVGDVGD
                     LSKLIMAKNNLRSKDNVDEDLAHELIDCLCSLLVLADKLGIDLDQAFIKTMDSLEKKL
                     TQKL"
     gene            1130..1285
                     /locus_tag="PKV52_03630"
     CDS             1130..1285
                     /locus_tag="PKV52_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99335.1"
                     /translation="MEDYNYESGPFVPAIPVIYIMVRIRQYSEMLAQHMALASEEAEW
                     RYTATMN"
     gene            1372..1506
                     /locus_tag="PKV52_03635"
     CDS             1372..1506
                     /locus_tag="PKV52_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99336.1"
                     /translation="MDDTMQEFVSWLKTDLTHFAALVEEGSVATDDPKQDGYSDMICR
                     "
     gene            1497..1661
                     /locus_tag="PKV52_03640"
     CDS             1497..1661
                     /locus_tag="PKV52_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99337.1"
                     /translation="MQIVVYKDITSEMLAVRNWLQQHPLGDNYLLSPRVYEEFIVGET
                     LNDISLKFIS"
BASE COUNT          612 a          298 c          385 g          514 t
ORIGIN      
        1 ttagtatata tgtcacaaga agtattaagg caatctgatg acgatttgcc gattaatgta
       61 gatgagcttc tcatagaaat acaactagct gcgaaagagc tcggcaatgg gcgtaagcat
      121 atggatgaac gccgtaaaat agcttatagg tatattatta aagaaaatat aattgcactg
      181 gggctagacc cccagaacta ctatggcgat taccgcgcat tgctacaggc tcgcccttca
      241 agtcaaattg ttgacgaaaa ctaattttag taacaaatat ttatctgatt cattattaag
      301 cttttattga actctgctga tgcccagtgg gtttttggtt cgtttgtgac cacccaaaaa
      361 tgaaaaccag aataaataaa ggttcttaaa atcaatgatg tttatttagt atcattcatt
      421 gaacgattgg ctgggtctgg gcatctcggc tcactattga ttcagctgat gtaacttcta
      481 gcatggccaa gacaaaaaga gtagtctggc acttcctaaa ataacaagtg tcagaccgac
      541 tgcaaggagt cctttttgtg ctatgtagaa gttactagca gatgagtatt gggagctggg
      601 cgcccagtgg gtttttggtt cgtttttgac cacacaaaaa tgaaaagtaa tataagtaat
      661 ataggtacta aaaagtacca gaaataagtt gcagtgtatc tacaaattat taatttatga
      721 aaagactata aaaagagtat ttattttcag tatcttttag gagtactatt agccgtatgg
      781 actataaaac tatgcaacaa cgggcactac agataagtaa cttgtacgat atgttaaata
      841 aaaaacatgc acaaaaaata tggaacgaac aagatgttat ggcaggtttt gtgggtgacg
      901 taggagattt atctaaatta ataatggcaa agaataattt gcgtagcaag gataacgtag
      961 acgaagattt agcgcacgaa ctgattgatt gtttatgttc tttgcttgtg ctggcagaca
     1021 agcttggtat agatttagac caagcattca taaaaacaat ggatagccta gaaaaaaagc
     1081 ttactcaaaa actgtagagt atgtagtagc aaaaatattt gttcattata tggaagacta
     1141 caattacgaa agtggcccat ttgttcctgc tatacctgtc atttatataa tggtgcgcat
     1201 acggcaatac agcgaaatgc tagcacaaca tatggcactc gcaagcgaag aagcagaatg
     1261 gcgctacact gccaccatga attaacacat aagagttaga ttttatagct aggaagaatt
     1321 agtagaatca tttggtttgg caatgcaaaa gcctcgtata tacttatgct tatggacgat
     1381 accatgcaag aatttgttag ttggctaaaa actgatctta ctcattttgc agcactggta
     1441 gaagaaggtt cggtagctac agacgacccc aagcaagacg gctatagcga tatgatttgc
     1501 agatagttgt atataaagat attacaagcg aaatgttggc ggtacgaaac tggctacagc
     1561 aacatccgct tggcgataat tacttgttga gtccacgtgt atatgaagaa tttatcgtag
     1621 gcgagacgtt aaatgatata tcactaaaat ttatatctta aactatttct ggcagggacg
     1681 tggtcgcaga aaagcagcca ccgagtaggg aagctgccat aaaaattggg tacgatggtt
     1741 tacaatattt ggttaaacga ttagagcgcc gctggctaaa cttagcccac tggtcaaatg
     1801 attacgcac
//
LOCUS       DAOPLS010000166         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_77117_length_1802_cov_4.145965, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000166 DAOPLS010000000
VERSION     DAOPLS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77117_length_1802_cov_4.145965"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="PKV52_03645"
     CDS             <1..1493
                     /locus_tag="PKV52_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPD99338.1"
                     /translation="VRKIARFSWQLAERGRLVPLTARGSGTDFSGGSLGQGVMIVFPA
                     HMNKVLTLDSNKGFVSVQPGINYGKLQQTLLTYGQFLPPYPASLEYSTVGGAVANNAA
                     GEKSVKYGVTKDYAKQLRVVLANGEVITTKRISKKELGQKKGLPSFEGELYRAIDNVL
                     NDSKKAIEGYVPSVTRSVSGYDIWSIKGNDGSFDLTPLFIGSQGTLGIVTEIVLDTET
                     YNPQKTLLVGFFDDIAKAGAAVNKLRELGPSTLEMIDGNLIKFLLEHNPNQINSVIKD
                     NSSKVVLFVEFDNLAARKQKKYTKKAKKVLEDFAVSFVATKDPTEQEDYWKVRHSAAA
                     VLWQNLGAKKALPVIEDAIFPTEKFAEFMQNVYSLFSNFQLDPPCVWGHAGSGNLHVQ
                     PFFDLSNIGDRQKMYKLMDSYYGMVLSMGGVITGAHNDGRLRAPYLGLQYGDEIYGIF
                     RKIKQICDPYGILNPGVKIDVLQKDTQQYVRSDYSLSHLYDHMPRT"
     gene            1549..1636
                     /locus_tag="PKV52_03650"
     tRNA            1549..1636
                     /locus_tag="PKV52_03650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1583..1585,aa:Leu,seq:gag)
     gene            1649..1725
                     /locus_tag="PKV52_03655"
     tRNA            1649..1725
                     /locus_tag="PKV52_03655"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Val,seq:tac)
BASE COUNT          562 a          350 c          416 g          474 t
ORIGIN      
        1 atgtcagaaa gattgctcgg ttttcgtggc agcttgcaga acgaggcagg ttagtaccac
       61 tgactgcaag aggctcaggt actgattttt ctggtggatc tttgggccaa ggagtaatga
      121 tagtatttcc ggcacacatg aataaggtat taacgctaga ctccaacaaa ggttttgtaa
      181 gcgtacaacc aggtattaac tatggcaaat tacagcaaac actactaaca tatggtcaat
      241 ttttgccacc ataccctgca tcgttagaat attcaacagt gggtggtgcg gtagctaata
      301 atgcagccgg tgaaaagtca gtaaaatatg gtgtaacgaa agattatgca aaacaattgc
      361 gggtggtgtt agcaaacggg gaagttatta ctaccaagcg aatatctaaa aaagagttag
      421 gccaaaaaaa aggtctgcct tcttttgagg gtgagttata tagagcaata gacaatgtat
      481 tgaacgatag caaaaaagcc atagaaggct acgttccatc agtgacaaga agtgtttctg
      541 gctatgatat ctggtctatc aaaggcaacg atggttcttt tgacttaaca cctcttttca
      601 ttggctcgca aggtacactg ggcattgtga cagaaattgt attagataca gaaacgtata
      661 acccccaaaa aacgctactc gttggtttct ttgatgatat tgcaaaagct ggtgctgcag
      721 tgaataaact gcgcgaactt ggcccgagca cactagaaat gattgatggc aacttaataa
      781 aattccttct cgagcataat cctaaccaga tcaacagcgt cattaaagat aactcatcaa
      841 aagtagtatt atttgtagag tttgataacc tggctgctcg taagcaaaaa aaatacacca
      901 aaaaagccaa aaaagttcta gaagattttg ctgtgtcatt tgtcgccaca aaagatccaa
      961 cagaacaaga agactactgg aaggtgaggc acagtgcagc agccgtgtta tggcaaaacc
     1021 taggtgcaaa aaaagcacta cctgttatag aagatgctat ctttccaact gaaaaatttg
     1081 cagaatttat gcaaaatgtc tacagcttat ttagtaattt tcaattagac ccaccgtgtg
     1141 tttggggcca tgctggcagt ggcaatttgc atgtacagcc gttttttgat ctttctaata
     1201 ttggcgatag acaaaaaatg tacaaactaa tggattcata ctatggcatg gtgctatcta
     1261 tgggcggtgt aattacaggt gcgcataacg atggaaggct gcgggcacca taccttggct
     1321 tacagtatgg cgatgaaata tatggtatat ttaggaaaat aaagcaaata tgcgacccat
     1381 atggcatttt aaatccaggg gttaaaattg atgtattgca aaaagacacg cagcagtacg
     1441 tgcggagcga ctattcgttg tcacacctgt acgaccacat gccaagaaca taaacaacta
     1501 cttgctatag gctataaaaa caggtatcat tgtacacgcg tatatcacgc gcgagtggcg
     1561 gaattggtag acgcgctggc ttgaggggcc agtgggcatt gcgctcgtgg aggttcaagt
     1621 cctctctcgc gcaccaagta tgcacgttgg gtgattagct cagttggcta gagcacctcg
     1681 tttacaccga gggggtcggg ggttcgaatc cctcatcacc caccacatta cattttttag
     1741 actactatgt ggctctttag ctcagttggt agagcagagg cctgaagagc cttgtgtccc
     1801 ca
//
LOCUS       DAOPLS010000167         1792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_77746_length_1792_cov_3.748993, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000167 DAOPLS010000000
VERSION     DAOPLS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77746_length_1792_cov_3.748993"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..553
                     /locus_tag="PKV52_03660"
     CDS             47..553
                     /locus_tag="PKV52_03660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPD99339.1"
                     /translation="MGTIYYNNYMTQSCEICTLLPNTKYVLLETDLWMVSLAPDQQFL
                     GRSFVTAKKHTRSLAALSKAQWDDLHQVIIALENAISSAYGADLFNWACLMNDAYKDT
                     ANTQSPHVHFHVRPRYKNTVYLQGYTFTDNDFGKHYKTPWDDPYQRAVPQTIMDAIYL
                     NIQEYLGS"
     gene            complement(689..1024)
                     /locus_tag="PKV52_03665"
     CDS             complement(689..1024)
                     /locus_tag="PKV52_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99340.1"
                     /translation="MTTIIVTCKFEDCREECTISAQEVVLVIHKDPEESFYSFMCTAC
                     KRYNKKQADEYTISLIEGYVTVTTVVEPSPVRYFCKDPISEHDIQCFVFDLADNALTG
                     AHIQEELEE"
     gene            1397..>1792
                     /locus_tag="PKV52_03670"
     CDS             1397..>1792
                     /locus_tag="PKV52_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99341.1"
                     /translation="MTTTLESLTMSGLQQVEEPTVLYEPYTYQPGDAETRLAALVAIT
                     QQNPMLELTEEKLRDGINVYIARVKHMGEWVVAGVCGSFLQYNDDTVREIGGVTVLPE
                     YRHGSYHDEHIGTTLAWLATESALHEGKTA"
BASE COUNT          544 a          384 c          382 g          482 t
ORIGIN      
        1 gcacgaacac cacccctcgt acctaatacg gcgaggggtt cttcttatgg gtacaatcta
       61 ctacaataat tacatgactc agtcgtgcga aatctgtaca ttattaccaa acactaagta
      121 tgtactacta gaaacagatt tgtggatggt aagtctcgcg ccagatcaac agtttttggg
      181 gcgtagtttc gtgacggcta aaaaacatac acgtagtttg gcagcattat ctaaagctca
      241 gtgggatgat ttgcatcaag ttataatagc actagaaaac gctatcagtt cggcctatgg
      301 tgcagaccta tttaattggg cttgtttaat gaacgatgca tataaagaca ccgcaaacac
      361 acagtcacca cacgtgcact ttcatgttcg gccacgctat aaaaatacag tttatctgca
      421 aggttatacg tttacagaca acgactttgg taaacactac aaaactccct gggatgaccc
      481 ataccaacgc gcagtaccac aaacaataat ggatgctatt tacctaaaca tacaagaata
      541 tttaggtagc taggtaaatg gccgctcttt taagggagcg gccatggtgg gtatgataag
      601 caggcacagc cgtgtagggg cgattgtgca ctcagggtac taccgtacac tggttgccct
      661 tgcgggttat acctgcatgg gctgccgatc actcttcgag ctcttcttgg atatgcgcac
      721 cggtaagtgc gttatctgca agatcaaaga cgaagcactg tatatcgtgc tcgctgatag
      781 ggtctttgca aaagtagcgt accggtgacg gctccacaac ggtagttacg gtcacgtacc
      841 cttcaatcaa cgagatggtg tattcatcgg cttgcttctt attgtaccgc ttgcaagccg
      901 tacacatgaa actatagaaa ctctcttctg ggtccttgtg gatcacaaga acaacctctt
      961 gtgccgagat tgtgcattct tctcgacaat cttcaaactt acaggtgact atgatcgtag
     1021 tcatgatatg cctccttagg ggcctgcttg tcaaagaact cgtagcgggc gctacaagat
     1081 aaaactatca tacaatcttc gtatattata gtcaatttct taactgttaa acaacatgta
     1141 aaagctcaca actaacccat aaaaattggt aataataggc atacgctatg cgctttttat
     1201 atgtaaaaaa tgttgctatt tagcggcaag tgtgctatag ttgtaactga tgagtacact
     1261 atatagttca gttgcaatcc gtcgtcgcac cattgggtgc taatctatat ttattgaaat
     1321 tatcaaaata tttaaagaat accacccctc aaacaaagag gggattttta atgattaagg
     1381 aatcaaccat acaattatga caacaacact agaatcactt acaatgagcg ggctacaaca
     1441 agttgaagaa cctacagttc tttacgaacc gtatacctat caaccaggtg atgccgaaac
     1501 tcgcttagca gcgttagtag caattaccca gcagaatcct atgctggaac tgactgaaga
     1561 aaaattacgt gacggcatta atgtctatat tgcacgagtg aagcatatgg gtgagtgggt
     1621 ggtcgctggt gtttgtggct catttttaca atacaacgac gatacagtca gagaaattgg
     1681 gggtgttact gttttaccag aatacaggca cggcagctac cacgatgaac atataggtac
     1741 tactttggca tggctggcta cagaaagtgc cttacacgaa ggcaaaacag ca
//
LOCUS       DAOPLS010000168         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_78531_length_1779_cov_4.316125, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000168 DAOPLS010000000
VERSION     DAOPLS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78531_length_1779_cov_4.316125"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKV52_03675"
     CDS             complement(<1..371)
                     /locus_tag="PKV52_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD99342.1"
                     /translation="MSTITPLAEQLRPQKIKDIIGQTKIVGKGEIIDHIIKTKQPVSL
                     ILWGPPGSGKTTIARIIANEVQAECIELSAVTSGIADVKKVISHAEANRRLGLQTVLF
                     VDEIHRFNKAQQDAFLPHEEKG"
     gene            complement(373..918)
                     /locus_tag="PKV52_03680"
     CDS             complement(373..918)
                     /locus_tag="PKV52_03680"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPD99343.1"
                     /translation="MNQLDTERSRQHDSRKTVYEFLLVIGQFTSLFIIVYPFSSIVLW
                     QATIFQLVGVMFVVMAIMLALWTLRSFKQKVNILPSPRKHSYLVTSGPYKYIRHPMYA
                     VLILGSFGIIIAYPTLVRLIGLCLLVSILHLKMKYEETMLSKKFTGYDTYQHATNKLI
                     PRAWHKPKTYKSSKKIVKKGT"
     gene            complement(921..1658)
                     /locus_tag="PKV52_03685"
     CDS             complement(921..1658)
                     /locus_tag="PKV52_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPD99344.1"
                     /translation="MQDKSGNPALRESVLDRVASLESTDSASIGGTTIKIFTLLSLSV
                     IGGFFGWQAMANASGATGFLIIGASLFALIMALTTVFKPQFAAVTGSLYALSQGYVLG
                     AISQTYNAALDGIVIQAIGLTGAILFVSLWAFSLGLIKVTHKFRIGVIIATASIALYY
                     VFAFIIGLFGGTAPLIFDSGTWGIVFSAVIIFVATLNLILDYDFIQTIVKRGAPKKME
                     WYAAFALIVTLLWLYIEVLRLLSKTRQ"
BASE COUNT          545 a          398 c          297 g          539 t
ORIGIN      
        1 cctttttctt catgtggtaa aaatgcatct tgttgagctt tgttaaaacg atgaatttca
       61 tctacaaata gtactgtttg caaaccgagc cgtctattcg cttcggcgtg tgaaataacc
      121 tttttaacat ctgcaatacc tgaggtaacg gcactaagtt caatacattc ggcctgcacc
      181 tcgttcgcta ttattcgtgc tatggttgtt ttgccactgc ctggtggccc ccacaatatc
      241 aagctaacag gctgcttggt ttttataata tgatcaataa tttcaccctt accgacaatt
      301 ttggtttgtc caatgatatc tttaattttt tgaggtcgca attgttcggc aagtggtgta
      361 atagtagaca ttctatgtac ctttttttac tattttctta ctagatttgt acgtcttagg
      421 tttgtgccac gcgcgtggta ttagcttatt ggtcgcgtgc tggtacgtat cgtagccagt
      481 aaacttttta gaaagcatcg tttcttcata cttcattttg agatgaagta tgctcactag
      541 caaacataat ccaataagcc gcacaagagt agggtaggcg ataattatac cgaaactccc
      601 aagtataagt acagcgtaca tcggatgtct tatatactta tacggaccac tcgtcactaa
      661 ataactatgt tttcgtggcg acggcaaaat gttcactttt tgcttaaaag atctcaaagt
      721 ccacaatgct agcataattg ccattactac gaacatgacg cccaccagct gaaaaattgt
      781 tgcttgccac aaaactatag agctaaatgg atacacaatg ataaataaac tagtaaattg
      841 cccaatcact aacaaaaatt catatacagt tttacggcta tcatgttgcc gtgagcgctc
      901 cgtatctaat tggttcatgc ttactgtctc gttttagaca gtaaacgtaa tacctcaatg
      961 tacaaccaaa gcaatgttac gatgagcgca aatgcagcgt accattccat ttttttagga
     1021 gcaccacgtt ttacaatcgt ctgaataaag tcataatcta aaattaggtt aagtgtcgcg
     1081 acaaaaataa taacagcact aaatacaata ccccacgtgc ctgaatcaaa aattaatgga
     1141 gccgtaccac caaatagtcc aataataaat gcaaaaacgt agtagagcgc tatgcttgca
     1201 gtcgcaataa tcacacctat cctaaactta tgcgttactt taattaaccc taaactaaat
     1261 gcccataaac ttacaaataa tatcgcgcct gttagcccta tagcctgaat aacaatacca
     1321 tcaagtgctg cattataggt ttgcgaaatc gctcccaaga catacccttg cgacagcgca
     1381 tacaaactac cagttactgc tgcaaactgt ggtttaaaaa cagttgtcaa tgccataata
     1441 agtgcaaata gtgatgcccc tattatcaaa aatcctgtag ccccacttgc gttcgccatt
     1501 gcttgccacc caaaaaatcc gcctataacg ctcaggctaa gcagtgtaaa tattttaata
     1561 gtagtcccac caatactcgc gctatcagtt gattctaacg aagcaactct atcaaggaca
     1621 ctttctctta gtgccggatt gccactctta tcttgcatat acgtttgtac ctcatttacg
     1681 tgtctatact aagtatacca atcaagcgct acgaaatact attttttacg taagttcttt
     1741 gccacccaaa atttagcaat attcagggaa ctacttggc
//
LOCUS       DAOPLS010000169         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_78963_length_1773_cov_2.491269, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000169 DAOPLS010000000
VERSION     DAOPLS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78963_length_1773_cov_2.491269"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKV52_03690"
     CDS             complement(<1..559)
                     /locus_tag="PKV52_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPD99345.1"
                     /translation="MQNNYNHNPPTTMHIDLNSCFAIIEQQANPLLRHKPVAIAAYDT
                     PRGMVIASSYEAKAKGIKIGINVQEARLLAPDVCVRMPDPSKYRDAHNRFKNVLLRYT
                     SSVTPKSIDEFVLDLQGSPQLRTGTTIQAIGQRIKQDIRDSLGSYVTVNVGIGTNRFW
                     AKTAAGLNKPDGLDTITARNVATVYQ"
     gene            586..777
                     /locus_tag="PKV52_03695"
     CDS             586..777
                     /locus_tag="PKV52_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99346.1"
                     /translation="MKSYEQGCLKDYPVEDGEFAKQCVHVGCNYEEFRKTDQQDGGEI
                     HVCIIGRLGHCAHPADIAS"
     gene            complement(774..1040)
                     /locus_tag="PKV52_03700"
     CDS             complement(774..1040)
                     /locus_tag="PKV52_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99347.1"
                     /translation="MKSKINQEISVVSFYNARNQRAQPYAISWQNKDYTVGEIGYHHK
                     VYDGKICHHIFELVDSEQSLWFRLNFNTDNLHSTLEEVSDGNAD"
     gene            1141..>1773
                     /locus_tag="PKV52_03705"
     CDS             1141..>1773
                     /locus_tag="PKV52_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPD99348.1"
                     /translation="MCGRYTLYGDDHLVERFNLATQPSFVSKDSYNIAPRQRLPIIYT
                     DDSQQRTAQLMQWGYIPPWSKDVKKERRPINTRAETVFTSPMWRSAVAHHRCLVPARG
                     FYEWKSEVTGKQPYYITPKTHDIFAFAGLYSIWHDAEHHPLYTFSILTTHPNAQMAKV
                     HDRMPVILLPEQESAWLNPTYRDQEQLAGLLEPYKDNGLEIYAVSSEVNTP"
BASE COUNT          469 a          398 c          410 g          496 t
ORIGIN      
        1 gctgatatac ggttgctacg ttgcgggcgg taatagtgtc tagaccatct ggcttgttaa
       61 gcccggccgc tgtttttgcc caaaacctgt tggtaccaat accaacattt accgttacat
      121 agctcccaag actatctctt atatcttgtt taatgcgttg gccaatggct tgtatggtgg
      181 tgccggtacg cagttgcggg ctaccctgaa gatcgagtac aaattcgtca atacttttgg
      241 gggtgacact actggtgtag cgcagcagca catttttaaa acggttgtgc gcatcgcggt
      301 acttactggg gtctggcatg cgtacgcaga catcaggcgc cagtaagcgt gcttcttgta
      361 cgtttattcc aatttttatt cccttggcct tggcttcgta gctgctcgca ataaccatgc
      421 cacgtggcgt gtcataggca gcaatagcca ccggcttatg gcgtaacagc gggtttgcct
      481 gttgttctat aattgcaaag caactattaa gatcaatgtg cattgtggtt ggtgggttgt
      541 gattataatt attctgcata ttgccacggt tgttatacta gatatatgaa gtcgtacgaa
      601 caaggctgct taaaagacta tcccgtagaa gatggcgagt ttgccaaaca gtgcgtgcac
      661 gtgggctgta attacgaaga atttcgtaag acagaccaac aagatggcgg tgaaatacac
      721 gtctgcataa ttggccggct tggccactgt gctcaccctg cagatatcgc cagctagtcg
      781 gcgttgccat cgcttacctc ttctagcgtc gagtgcaaat tatcggtatt aaaattaagc
      841 cgaaaccaca ggctttgttc actgtctaca agttcaaata tatggtggca gatcttgcca
      901 tcgtatacct tgtggtggta gccaatttcg cctaccgtgt aatctttatt ttgccaactg
      961 atggcgtacg gttgagcacg ctgattacga gcattgtaaa acgataccac gcttatttct
     1021 tggttaattt tagatttcat acatatcctt tagagttatc agttttaaga cacacatata
     1081 cgtagtgcca aggagccttc agctatttac taaacgctca cgacgaaata ttattaacgt
     1141 atgtgcggac ggtatacatt gtatggcgat gaccacttgg tagagcggtt taatttggct
     1201 acacagccgt cttttgtaag taaagatagt tacaatattg cgccccggca gcgtttgcca
     1261 attatatata cagacgattc acaacagcgc acggcccaat taatgcaatg ggggtatatt
     1321 ccaccttggt caaaagatgt aaaaaaagaa agacgaccca ttaatacacg cgcagaaact
     1381 gtatttacaa gccctatgtg gcggagtgct gtcgcacatc accgttgctt ggtgcctgca
     1441 aggggctttt atgaatggaa gtcagaagtt acaggtaaac agccctacta tattacgcca
     1501 aaaacacacg atatatttgc ctttgcgggg ctttatagta tttggcacga tgccgaacac
     1561 cacccgctat atacgttttc tatccttaca acgcacccca atgcccaaat ggccaaagtg
     1621 cacgatagaa tgccagttat actactgcca gagcaagaat ctgcttggct aaaccctacg
     1681 taccgcgacc aagagcaact ggcgggcttg cttgaaccgt acaaggataa tggcttagag
     1741 atatatgcag ttagctcaga agtgaatacg cct
//
LOCUS       DAOPLS010000170         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_79216_length_1769_cov_3.224621, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000170 DAOPLS010000000
VERSION     DAOPLS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79216_length_1769_cov_3.224621"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKV52_03710"
     CDS             complement(<1..539)
                     /locus_tag="PKV52_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99349.1"
                     /translation="MSARDWRFIVFICLLIGAPFSKYPSIAIPLFNFPSFRIGAYQLV
                     AAVFVLLCVKPIYHAGATFFKSYHKLTFYSLAAFSALTAISVLWSLYPARSALLAVSV
                     LMLVAIILAGWWFAANELSNKRKIIMVHALLYASIGFGIIALIQLVIFTLTNSTLGVL
                     CPGCTAEVFGFPRVNGLAA"
     gene            611..1045
                     /locus_tag="PKV52_03715"
     CDS             611..1045
                     /locus_tag="PKV52_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPD99350.1"
                     /translation="MRIKKKTRKLITQIVEYMIAGGAWFWSGYIIISVLDDKIALFWA
                     NFIGNAVGISINFILNKYWVFKTKKQSDLLNTTWRYVIYTALNAFLLNYLILQGLRDI
                     GIEPEIGQFLAAGFFTVWNFIWYKVWVFKGQPKPKKRIKHHA"
     gene            1038..1487
                     /locus_tag="PKV52_03720"
     CDS             1038..1487
                     /locus_tag="PKV52_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99351.1"
                     /translation="MPDAIRPTKRQLELLDFIGAFIAQHGYSPSYREIMVGCHYTSVA
                     TVALHVNNLIARGHLVKRGRSARSLEVTDAKVIAPHPSTEVTQSQAKWLVVLIDNRFT
                     IAESQPSITQQQLDELYVLVGSLKVLGLQDAATSFMPRLKNLHEHLV"
BASE COUNT          593 a          325 c          366 g          485 t
ORIGIN      
        1 tctgccgcta agccgttcac ccgaggaaag ccaaatactt cggccgtaca accagggcat
       61 agaacaccga gtgtgctatt ggttagcgta aaaataacca attgtataag agcgattatg
      121 ccaaatccaa tactggcata cagtagagca tgtaccataa ttatctttct tttattagaa
      181 agttcgttag cggcaaacca ccaaccggca agaattatcg ccacaagcat taaaacagat
      241 actgcaagca acgcacttcg cgcaggatat aaagaccaca ggacacttat agcggtaagt
      301 gcagaaaatg ctgccagtga gtaaaaggtt agcttatgat aacttttaaa aaaagtagca
      361 ccggcgtggt agattggttt aacgcataac aatacaaaaa ctgctgcgac aagctggtag
      421 gcaccaattc tgaatgacgg aaagttaaat aacggaatag caatgctagg atatttagaa
      481 aatggtgctc caattaagag acagataaaa acaataaaac gccaatcgcg tgctgacatt
      541 gaacccataa ctattctatt gtagcgatgt tagctgtgat acactagtaa aagaacaaat
      601 tgcaaacact atgcgtataa aaaagaaaac cagaaaactc attacccaga ttgtagaata
      661 catgattgct ggtggcgcat ggttttggag tggctacata attatatctg ttttagacga
      721 caaaattgcc ttgttttggg cgaattttat tggtaacgca gtgggtataa gtattaattt
      781 tatccttaat aaatattggg tgtttaaaac taaaaaacaa tcagatttat taaatactac
      841 ttggcgatat gttatataca cagctctaaa tgccttttta ctaaactatt taatactgca
      901 aggattacgt gatattggca tagaaccaga aataggccaa tttttggcag ctgggttctt
      961 tacggtgtgg aattttatct ggtataaagt atgggtgttt aaagggcaac caaagcctaa
     1021 aaaaaggatt aaacaccatg cctgatgcta taagaccgac caaacggcaa ctagagcttc
     1081 tcgattttat aggtgcgttt attgcacagc atggctatag cccaagctac cgtgaaatta
     1141 tggtggggtg tcattacact tctgtggcga ctgtggcgct gcatgttaat aatttaatag
     1201 cacggggcca tttggtaaag cgtgggcgtt cggctcggtc gttagaggtt actgacgcta
     1261 aggtaatagc ccctcatccg tcaacggaag ttacgcagtc gcaagcaaaa tggctggtgg
     1321 tactcataga taacagattt acaattgcag aaagccaacc aagtattact cagcaacagc
     1381 tcgatgaact atatgtttta gtaggatcat taaaagtatt aggtttacaa gacgctgcca
     1441 cgagctttat gccacgattg aaaaatttac atgagcatct agtttaataa ttattctgta
     1501 agaaaatgta attatgaggg tagtgtaata aagaaagcac cgcgataatt atctttatat
     1561 cgttatataa aaaacacctc gcatagctag tgcgaggtgt tttagttata gaagcctatt
     1621 atttcttgtc ttctttgact tctactgttt ctatttctaa gccaccacca acgcttaaca
     1681 cagcgttcca aataattgct atgagtgcgc cgtatagcca gccaaatgca aaccaaatca
     1741 gtgggtatac aatgagggca aataaagcg
//
LOCUS       DAOPLS010000171         1761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_79775_length_1761_cov_2.079132, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000171 DAOPLS010000000
VERSION     DAOPLS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79775_length_1761_cov_2.079132"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1761)
                     /locus_tag="PKV52_03725"
     CDS             complement(<1..>1761)
                     /locus_tag="PKV52_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99352.1"
                     /translation="IPFFAKGTDSLPFDAQTIAKTSGIPHVVIYRKATGNTAPSGNPD
                     VPDYNKTPKLAAQEHWTWHRSQLPPELDKAVTWVETINEVDKNRAEWLAEFAIETAKL
                     TMADGYKWLAFGWSTGEPEPAHWQGVKMRQFLDMVAQNPDKLGIAVHEYSLDVNQINV
                     PYLMGRYKNILDVNPNVNIYVTEWGWTYNAAPNMTKAMADITAIADVYNHPRLKGCAI
                     WALNGGWGNIAQTVKTYITPLKELTLNTVLPDPSDSTFNYSFENGWTDVAPAPDRLQQ
                     PRDWQLTVLEPGEPIWGNNAVTGTPSIVTGIPECIHKHNNQLPPNQQFGGSDALILDG
                     NYVYKIQAVHMVCGVKLSTTITGLSPNTNYNVTVPTQVHYQNDYPDPEPDDIEIWIND
                     SHYLAEILPDREWLPLETIVTSNSSGSAVLDIRFMTKWGNSRDMFIDNITLYEVADPP
                     PTTPGLGYKSIPVLLPQDATLSEIIDVRTAEFANRRTITQSHDEVIQLVKLGKAGSYA
                     IIYDIHRWPVENQQQLLAIPHELRTLTPTAVKLTLPILYSQRNTAWASKCIGVCPNDK
                     TIGNWGCLLTAYNMLAQYLGL"
BASE COUNT          450 a          388 c          371 g          552 t
ORIGIN      
        1 caaacctaga tattgcgcta acatgttata tgcggttaac aaacaacccc aattaccaat
       61 tgttttgtca ttagggcaaa caccaataca cttactagcc caagcggtgt tacgctgact
      121 atataaaatt ggcagtgtca acttaacggc tgttggggtt aatgtacgca attcatgggg
      181 aattgctaat agttgttgct gattttcaac aggccaacgg tggatgtcat atatgattgc
      241 atacgatccc gctttgccga gtttaaccaa ctgaataacc tcgtcatgag attgtgtaat
      301 tgtacggcga tttgcaaatt cagcagtacg cacgtcaatt atttcactta gagtcgcatc
      361 ttgtggcaat aacacaggta ttgatttata acccaatccc ggtgttgttg gtggcgggtc
      421 tgcaacttca tacaatgtaa tgttgtcgat aaacatatct cgtgagttac cccattttgt
      481 catgaaacgt atgtccaata cagcactacc gctactgtta cttgtcacaa tggtttccaa
      541 cggcagccat tcccgatctg gcaagatttc agctaagtaa tggctatcgt taatccaaat
      601 ttcgatgtcg tctggttcag gatcagggta gtcattttga taatgtacct gtgttggtac
      661 ggttacatta tagtttgtat tgggacttaa ccctgttatg gttgtactta attttacccc
      721 gcaaaccata tgcacagctt ggattttgta cacataatta ccatccaaga ttagtgcatc
      781 actgccacca aactgctgat taggtggtaa ctggttattg tgtttatgaa tgcattcggg
      841 aatacctgta acaatacttg gcgtacctgt taccgcatta ttgccccaaa ttggttctcc
      901 tggttctaaa acagtcaatt gccagtcacg cggttgctgt aaccggtctg gcgctggcgc
      961 aacatctgtc cagccgtttt caaagctgta attgaatgtg ctatcactcg gatcgggtag
     1021 gactgtattt agtgttagct ctttcaaagg cgtgatatat gttttcactg tttgggcaat
     1081 attaccccag cccccattca atgcccaaat cgcacaccct ttaaggcgtg gatgattgta
     1141 tacatcggca attgccgtaa tatcggccat tgccttagtc atatttggcg cagcattata
     1201 agtccaaccc cactcagtta cataaatatt tacgttaggg ttaacgtcaa ggatattctt
     1261 ataacgcccc attagatatg gcacgttaat ttggtttaca tctagggaat actcatgtac
     1321 ggcaatacct agtttgtcgg gattttgtgc aaccatgtct aggaattggc gcatttttac
     1381 accttgccag tgtgcgggtt cgggttcccc ggtagaccaa ccaaatgcca gccacttata
     1441 accatctgcc attgtcagtt tggccgtttc aattgcaaac tcagccaacc attctgcacg
     1501 gtttttgtca acttcgttaa tggtttcaac ccaggtaaca gccttgtcaa gttcgggcgg
     1561 caattgcgat ctgtgccatg tccaatgctc ctgcgctgcc aattttggag ttttgttata
     1621 atccggcaca tcgggattcc cggatggtgc agtattacct gttgctttac ggtaaataac
     1681 aacgtgtggt attccgcttg tttttgcaat ggtttgtgca tcaaaaggta aactatcagt
     1741 acctttcgca aaaaatggga t
//
LOCUS       DAOPLS010000172         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_80753_length_1746_cov_3.562389, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000172 DAOPLS010000000
VERSION     DAOPLS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80753_length_1746_cov_3.562389"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1169
                     /locus_tag="PKV52_03730"
     CDS             288..1169
                     /locus_tag="PKV52_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99353.1"
                     /translation="MKKFLVAAALAFAMVPAFGFVNAASAVEDYGSIGGGNIVTSKNV
                     TKNGTFADVTNATCSDVVKIRAYVHNPGPNDLTGVRAQAVLPTGQATSFSVKMTISAA
                     NANPKSESDTTSIKSDKTASMSYVAGSAEYFDANGARLGSLSDSIVTSGTDVPGGVGV
                     STMNARYVQFQAKLNCDTPPPVTITVCDLTTKQIVTIKESEFNTEKYSKDLADCAQPG
                     EITVCDLTTKKIVNIKEDEFNSETYTKDLTLCVVTPVTPPTSIPNTGAGSVLGIAAVV
                     SALGTIGYRLWMVKRLQ"
     gene            1351..>1746
                     /locus_tag="PKV52_03735"
     CDS             1351..>1746
                     /locus_tag="PKV52_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99354.1"
                     /translation="MSIATKFLMGIASVFALAIVALIAMPAKAVNIDTTRDCDKYAVM
                     YCGSMTKAEIVKKLQNGDGQNSAKNIKDIYSKFGLSVKDIEGAKFEDGVVYKDGDVKI
                     GNKIVAKDAKTYIRTMGKVSTSKMGTAQAA"
BASE COUNT          557 a          335 c          377 g          477 t
ORIGIN      
        1 gccaccaagg gtacttccgt gcccaggctg aaggaaacgg cactctcaca ggtaccgctt
       61 ctgacagcat ggggggaatg tttaaccccc aacccttgtc gttcgtctcg gtgtttatca
      121 ttcaccctga cacctgatag atgtagtagt tagtgttagc gggtgcgtac acagtatatg
      181 gctttcacca gccgtagcct gtgtgcgccc cgcgcactaa ccaagactta aatgactatt
      241 tagtttgcaa aattatcaat tgagtagttt tttattaagg aggaactatg aaaaaatttc
      301 tagttgctgc ggcacttgca tttgcaatgg taccagcatt tggttttgta aacgcagcat
      361 cagctgtaga agattacggc tccattggag gcggtaatat agtaacttct aagaatgtta
      421 ctaaaaatgg tacgtttgcc gatgttacta acgctacttg tagcgacgta gtaaagatcc
      481 gtgcatatgt acacaaccct ggtcctaacg accttactgg cgtacgcgct caagctgttt
      541 tgccaactgg gcaagcaaca agttttagcg taaaaatgac tattagcgct gctaacgcta
      601 atccaaaatc agaatcagac acaacaagca taaaaagcga taaaactgct tctatgagtt
      661 atgtcgccgg tagtgcagaa tattttgatg caaacggtgc acgacttggt tcattaagcg
      721 atagcattgt gacatctggt actgatgtac ctggtggcgt cggtgtatct actatgaacg
      781 ctcgttatgt acagtttcaa gcaaaactta actgtgacac gcccccacca gtaacgatta
      841 ctgtttgtga tttaactacc aaacaaattg ttacaattaa agagtctgag tttaatacag
      901 aaaaatactc aaaagactta gcagattgtg cacagccagg tgaaattact gtatgtgatc
      961 taactactaa aaaaatcgtc aacattaaag aagacgaatt taatagtgaa acatacacta
     1021 aagaccttac actgtgtgtg gtaacaccag taactcctcc taccagtatc ccaaatactg
     1081 gtgcaggctc ggtacttggt atcgcagctg ttgtatcggc acttggtaca attggctacc
     1141 gtttgtggat ggttaagcgc ttacaataag catctgtaag cacgagactg accgctatac
     1201 ttacaaaaaa atacccctat cggtaaaatg gtaggggtat ttttataggt acaaaccata
     1261 agagtattga caaataaaca aaagcgtgat acaatatgca taacagttga ttatcaactg
     1321 gtgtgtaagg taataaggag gctcaatata atgagtatcg caacaaagtt tttaatggga
     1381 attgcatctg tttttgcact tgcaattgta gcgcttatcg ctatgccggc taaggccgta
     1441 aacatagaca ctacacgtga ctgtgataaa tatgcagtta tgtattgtgg gtcaatgaca
     1501 aaagcagaaa ttgttaaaaa gctacaaaat ggcgatggcc aaaatagtgc taaaaatatc
     1561 aaagatattt atagtaagtt tggtttaagc gttaaagata tagaaggcgc aaagtttgaa
     1621 gacggcgttg tctataaaga tggtgatgta aaaattggta acaaaattgt tgccaaagac
     1681 gctaaaacgt atattcgcac catgggtaag gtaagtacca gtaaaatggg taccgcccaa
     1741 gctgct
//
LOCUS       DAOPLS010000173         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_81094_length_1741_cov_2.487544, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000173 DAOPLS010000000
VERSION     DAOPLS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81094_length_1741_cov_2.487544"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PKV52_03740"
     CDS             <1..836
                     /locus_tag="PKV52_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99355.1"
                     /translation="LSDATGVATAASDVVTIVVSGVSNPSDVDTVTVGNQVGTFYGRM
                     LTFNDSAQVANYLATNGTTATGVVDFGGSAMSTTNDLTVTARVQENIRFCLYTNGPAQ
                     TCATGTGNAIDIPDATTPLNANSVSTANAYFNVSSNALSGVNIRMWSNNGSDGVLKSG
                     VYTISAFGPTVDDVCTANSANTSVEQFGLRINPGASVTAVAPYNCAAGNHGWDADNTA
                     GSGANDATTVGSAYGDTVATTAGANDASESTLEFAAKSALTTEAGIYTTALNYIATGT
                     Y"
     gene            1069..>1741
                     /locus_tag="PKV52_03745"
     CDS             1069..>1741
                     /locus_tag="PKV52_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99356.1"
                     /translation="MANPIAFIKRPAHIAAALLFGFALVVPVVLSATSQAAQLQSRSI
                     QLANSATGATGVSYTVTFNVAQTATIKGIVVDICSGNTSPLIGTACSQPTSFTWGGSP
                     SITSATINATDISTWTWASANGGRTLTATDATGVAATSGQTVTIVVASVANPSDVDVV
                     TGGNQVGTFYGRMITFSDNTQVASYAPANGEATTGYLDVGGAAMSTTNNLTVTARVQE
                     NIRFCL"
BASE COUNT          510 a          389 c          390 g          452 t
ORIGIN      
        1 cgcttagcga cgctacaggt gtagcaaccg ctgctagcga tgtagtaact atcgtggtaa
       61 gcggtgttag taacccatca gacgtagaca cagtaactgt tggtaaccaa gtcggtacct
      121 tttacggcag gatgctaaca tttaacgaca gtgcacaagt tgcgaactat ttggcaacaa
      181 atggtacgac ggcaaccggc gtagttgact ttggtggttc tgcaatgagc accacgaacg
      241 atctaactgt tacggcccgc gttcaagaaa acattcgttt ttgcctatac actaacggtc
      301 ctgcacaaac atgtgcaaca ggtacgggta acgctataga cattccagac gccacaacgc
      361 cacttaacgc taacagcgtt tctactgcaa atgcctactt taacgtatct agtaacgcat
      421 taagcggtgt aaacatccga atgtggagta ataatggtag cgacggtgta ttaaagagtg
      481 gcgtttacac tattagtgcc tttgggccta cagtagacga tgtctgtaca gctaactcag
      541 ctaatacatc ggtagaacag tttgggctac gtattaaccc gggtgcaagc gtaacagcag
      601 ttgctccata taactgtgct gctggtaatc atggttggga tgcagataac acagctggtt
      661 ctggcgccaa cgacgccacg acagttggtt cagcttacgg tgacaccgta gcgacaacag
      721 caggtgcaaa cgatgcaagt gaatctacat tagaatttgc tgccaaatct gccctaacta
      781 cagaggctgg tatttacacc acggctctta actatattgc aacaggtacc tattagtacg
      841 taacataaca acaacgtttt gactgacata tatatgatgt agacattgag gattgtaaaa
      901 attaagaata acgcatccat ttttggtaag ggggatgcgt tatttctttt gtagcacctg
      961 tggataagtt aggcataaat gcgttgaact attattcata aactgctaat gtatatttgt
     1021 atattaactt aaattaaaaa ccaaaaaaca ataaaaagga gaaaaagcat ggccaaccct
     1081 attgctttta taaaaagacc agcacatatt gctgctgctt tattgtttgg atttgcttta
     1141 gttgtgcccg ttgtacttag tgccacgagc caagcagccc agctgcaaag cagatctata
     1201 caattagcga attcagccac aggtgctacc ggtgtgtcat acactgttac atttaatgtt
     1261 gcccaaaccg caaccataaa aggtatcgtc gtagatatct gttctggtaa taccagccca
     1321 ctcattggta cggcgtgttc acaacccacc agctttactt ggggtggttc accttccata
     1381 acgtcggcta ctataaatgc aacggacatt agtacctgga cgtgggcgtc agccaatggc
     1441 ggccgaacac taacagctac agatgctaca ggtgtagcag caacatctgg tcagacagta
     1501 acgattgtcg tcgctagcgt tgctaaccca tcagacgtag atgtcgtaac aggtggtaac
     1561 caagtcggca cattctacgg cagaatgatc acctttagcg ataacacaca agttgcaagc
     1621 tatgcgcctg caaacggtga agctaccaca gggtatctag acgttggtgg tgctgcaatg
     1681 agtacaacca ataaccttac tgttaccgct cgtgttcagg aaaacattcg tttttgccta
     1741 t
//
LOCUS       DAOPLS010000174         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_82563_length_1720_cov_3.037237, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000174 DAOPLS010000000
VERSION     DAOPLS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82563_length_1720_cov_3.037237"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1720
                     /locus_tag="PKV52_03750"
     CDS             <1..>1720
                     /locus_tag="PKV52_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPD99357.1"
                     /translation="WTNGASPYFTWDAGSDGQGGVRGYCAYVGQDSMADPTTTKGLLG
                     NSPTATGGNCQFLVSGTNLDLATPGYMQTPMTSSNSPYYLTLRAIDMAGNVAATSMQF
                     SFRFDNTPPTNPTFITAPSGFVNTKQVTMTWPTTGPGAAQDDNSGLVGLQYKIGINGT
                     WYGDFHTGTQEINDILANDGSYMTQDPIDFNNLTEGINTVYFRTWDAAGNVTTNYTTA
                     TLKINTSGAPSEPNNLTATPSYSTTNSFGFNWDTPTTFIGDSNNLTYCYTVNTTPSAG
                     SCAYTAAGSTVLSLGPYATQPGQNTLYVVAKDESNNINYANYASVTFTANTPSPGIPR
                     NTDIVDVSIKATSNWRLALTWEEPTNAGAGIANYKVYRSNDNVTFSQVGSSSSTTYID
                     AGLSKQRYYYRVTACDSTNNCGATGTVVYETPTGKFTSPAGITSQPVINNVTTKKATV
                     KWATDRASDSKIAIGTKSGEYSSSEVGNSAQVTDHTLRLDNLAPGTTYYYVAKWTDED
                     GNTGKTTEKSFTTLPAPSIKEVTASNVSLSGATIDFTTRGATKATVYYGTSESFGGII
                     NVNTST"
BASE COUNT          536 a          381 c          380 g          423 t
ORIGIN      
        1 attggacaaa tggtgcttca ccatacttta cttgggatgc tgggtcagac ggtcaaggcg
       61 gtgttagggg atactgcgca tacgtagggc aagatagcat ggccgaccca acaactacga
      121 aaggtctact gggcaacagc cctactgcta ctggtggcaa ctgccaattt ttggtgtccg
      181 gcacgaattt agatttggca actcctggat atatgcaaac tccaatgact agctctaaca
      241 gcccatatta tcttacgttg cgtgcgatag atatggctgg caacgttgcg gcgacatcta
      301 tgcagtttag ctttaggttc gataatacac cccctactaa cccaacgttt ataactgccc
      361 cgtctggttt tgttaacacg aaacaagtta ccatgacatg gccaacaact gggcccggtg
      421 ctgcacaaga tgacaactcg ggtttggttg gcttgcaata taaaataggc ataaatggca
      481 catggtatgg tgactttcac actggcactc aagaaataaa cgatatatta gccaatgacg
      541 gctcatatat gacgcaagat ccaatagatt ttaataattt aacagaaggc attaacacag
      601 tatattttag aacatgggat gccgctggta acgttacgac caactacact acagctacgt
      661 taaaaatcaa cacaagtggc gcgccaagcg aaccaaataa tctaacagcc acgcctagct
      721 atagcacgac gaattcattt ggttttaact gggatacacc tacaacattc attggcgata
      781 gtaacaacct aacgtattgc tacactgtta ataccacacc atcggcgggt tcatgtgcct
      841 atacagcagc aggctctaca gtcttaagtt taggaccgta cgctacccaa ccgggccaaa
      901 acactttata tgtagtagca aaagatgaat caaataatat taattatgcc aactatgctt
      961 cggttacttt cacggcgaat acaccatcgc ctggcattcc ccgcaataca gatatcgtgg
     1021 atgtttctat taaagcaaca agtaactgga gacttgccct gacgtgggaa gaaccaacga
     1081 acgcgggcgc tggtatcgct aattacaagg tgtaccgatc gaatgacaat gttacatttt
     1141 cacaagttgg cagtagctct agcacaacat atatagacgc aggcttatcc aaacagcgtt
     1201 attactatag ggtaactgcg tgtgatagta ctaataattg cggtgctaca ggaacggttg
     1261 tgtatgaaac tccaactggt aagtttacga gcccagcagg catcacgagc caaccggtca
     1321 taaataatgt gacgactaaa aaagcgactg tgaagtgggc gacagataga gcgagtgatt
     1381 ctaagatagc aatcggaacg aaaagtggcg aatacagttc tagcgaagtt ggtaattctg
     1441 ctcaagtaac agatcataca ctgcgacttg ataatttagc acctggcaca acgtattact
     1501 acgtagctaa gtggactgat gaggatggta atactggcaa aactacagaa aaaagcttta
     1561 ctacattgcc tgccccatca atcaaagaag ttacagcgag caatgtcagc ctatctggcg
     1621 caacgattga ttttacaacg aggggcgcca caaaggcgac ggtatattat ggcacaagtg
     1681 aatcgtttgg tggcattatt aacgttaata cttctaccag
//
LOCUS       DAOPLS010000175         1708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_83448_length_1708_cov_3.024198, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000175 DAOPLS010000000
VERSION     DAOPLS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83448_length_1708_cov_3.024198"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..498
                     /locus_tag="PKV52_03755"
     CDS             85..498
                     /locus_tag="PKV52_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99358.1"
                     /translation="MTDIPKIKLKVSNYGLKYMEKFSTGEYYIKYPSRHETLIFRVYT
                     PELFLVAVTDILADIKLFHYPWKEPIKLEMTPEIEAVGEQFPDIIKKIEESQKEYKEW
                     YEHQQIALYFDEYDPVALFWYLGDTFGVQVEEFDN"
     gene            complement(547..>1708)
                     /locus_tag="PKV52_03760"
     CDS             complement(547..>1708)
                     /locus_tag="PKV52_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HPD99359.1"
                     /translation="AEWDATTDLAIERGAMPLWYEQGFLPTEFTAFLADIHPDAHKRM
                     LQYGRRNISVSTVAPTGSLSILAKLVTEYGTTAGIEPLFAPWYTRRRKVNADAEKVDF
                     VDATGDHWLEFKVYHNGLLEWAKINGVADIVADYDKSPYYKACAPDIDWEQRVAIQGL
                     VQKFVTHSISSTVNLPKTATIADVDKIYRTAYAKGLKGITVYVDGSRDGVLITTSTTP
                     KIAKNDAPKRPARLDCKIFNTTVKGEYWVVLIGMLNDEPYEVFAYPNGRINYREGVIL
                     KRGKGVYDLEVNGKVLHNDIASLYVNDEQAAATRLISTALRHGTDAIFVIEQLEKSGG
                     TIVDFSKAIARALKKMLVVTPRKATDCPDCGSELVSESGCLTCKNCGYSKCG"
BASE COUNT          520 a          346 c          327 g          515 t
ORIGIN      
        1 aagtaagcat agtttaatta taaaggttta tttgaaacat ggcaagggct tgacaagccc
       61 ttttttaatt tttatactaa tgtcatgaca gacataccga aaatcaaact caaagtatca
      121 aactacgggt tgaaatatat ggaaaagttt agcactggtg aatattacat caaatatcct
      181 agtcgccacg aaacacttat ttttcgggta tacacacccg aacttttctt agtggcggtc
      241 acagatattt tagcggatat aaaacttttc cattatccct ggaaagaacc gatcaaactt
      301 gaaatgacac ctgagattga agcggttggg gaacaatttc cagacattat taaaaaaatt
      361 gaagagagtc aaaaagaata taaagagtgg tatgaacacc aacaaatcgc tttgtatttt
      421 gatgaatatg atcctgttgc cctattttgg tatttgggtg atacgtttgg ggtacaagtc
      481 gaagaatttg ataattaaaa aaggctacta tttagtagcc tttttttgtt tataccctat
      541 ctaatactac ccacacttac tatacccaca gtttttacaa gttaaacagc ctgattcact
      601 aacgagttca ctaccacaat cggggcaatc ggtagcttta cgcggtgtaa caactaacat
      661 ctttttcaaa gcacgggcga ttgctttaga aaagtctaca attgtgccgc ctgatttttc
      721 aagttgttca attacaaaaa tagcatctgt gccatgtcgt aaagctgtac taattaaacg
      781 tgtggcagcg gcctgttcgt cattgacgta taaactagca atatcgttat gtaaaacctt
      841 gccgtttact tcaaggtcat aaaccccttt gccacgtttt agaataacac cttcgcggta
      901 attaatacgc ccattgggat aggcaaagac ttcgtaaggt tcgtcgttca acataccaat
      961 caatacaacc caatattcac ctttaacggt ggtattgaag attttacagt caagtcttgc
     1021 ggggcgtttt ggggcatcgt ttttagcaat cttgggtgtt gttgaggttg tgattaaaac
     1081 accgtcccga ctaccatcaa catagactgt gatacctttt aagccttttg cgtaagctgt
     1141 gcggtaaatc ttatcaacat ctgctattgt agcagttttg ggtaaattaa cagttgatga
     1201 aatagaatgg gttacaaatt tttgcactaa accctgaatt gcaacgcgtt gttcccaatc
     1261 aatatcaggg gcacatgcct tgtaataggg tgatttatca taatcagcta cgatgtcggc
     1321 aacaccattg attttagccc attctaatag cccattatga tataccttaa actcaagcca
     1381 gtgatcacct gttgcatcta caaagtcaac tttttcagca tcggcattga ctttgcggcg
     1441 acgtgtatac cagggtgcaa acaatggttc aataccagca gttgtgccgt attctgtaac
     1501 taatttagct aagattgaca acgaacctgt cggtgcaacc gtactaacac taatattacg
     1561 tcggccatat tgcaacatgc gcttgtgggc atcggggtgt atatcagcca aaaaggcggt
     1621 aaactcggtt ggtaaaaagc cttgttcata ccacaaaggc attgccccac gttcaatcgc
     1681 caaatctgtc gtagcatccc attctgct
//
LOCUS       DAOPLS010000176         1708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_83472_length_1708_cov_1.757411, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000176 DAOPLS010000000
VERSION     DAOPLS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83472_length_1708_cov_1.757411"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..453
                     /locus_tag="PKV52_03765"
     CDS             253..453
                     /locus_tag="PKV52_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99360.1"
                     /translation="MNTIEFMQTIQNNNFKMRVSGFAQNARNNQPEAVTGVVTKVGQS
                     VFYIGSKRYRPFMNQTKVLPFK"
     gene            463..535
                     /locus_tag="PKV52_03770"
     tRNA            463..535
                     /locus_tag="PKV52_03770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:496..498,aa:Ile,seq:cat)
     gene            583..653
                     /locus_tag="PKV52_03775"
     tRNA            583..653
                     /locus_tag="PKV52_03775"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:615..617,aa:Trp,seq:cca)
     gene            822..1184
                     /locus_tag="PKV52_03780"
     CDS             822..1184
                     /locus_tag="PKV52_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99361.1"
                     /translation="MNERAQNLLNILNTALNTGDALALVKARNYLALLCDIYTEDIYL
                     AMDAVASEWALVWGLRFAHNTVVLFTSYYDLWRYLQVKETNPTAYLDDNFVTYVAMCD
                     INESEVHILRDKHFAIVF"
     gene            1286..1645
                     /locus_tag="PKV52_03785"
     CDS             1286..1645
                     /locus_tag="PKV52_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99362.1"
                     /translation="MHQINLPTNEFWVEYKGFTIIEDWQSWRIFKGKQVLYTVKTLDE
                     GRQFVDKALEAAALQGDGRGLGFAVIIAEQLTGGYWFGVQYHHTDKVQMFEWQAVTIH
                     SAMYPDFSHNLMAHVQI"
BASE COUNT          527 a          316 c          342 g          523 t
ORIGIN      
        1 gcttacaaat tttccccaat aaagcagtta ctttactaat ccccgaattc acttgtacgc
       61 taactggtaa aaggtattca aactggcaat gacacttaat gcggcggttt ggcttatttt
      121 actaattttt gtaattttac ctttggtgct aatggtggtt ttagatgatt agtaccaaag
      181 tcaggttgac caacaacaat tttagcttta tactttaatc aagattataa aataaagttt
      241 aggagattaa aaatgaacac aatcgaattt atgcaaacaa tccaaaataa caatttcaaa
      301 atgcgcgtta gtggttttgc ccaaaatgca cgcaacaacc aacctgaagc ggttacgggg
      361 gttgtgacaa aagtaggcca aagtgtcttt tatattggtt caaaacggta tcgcccattt
      421 atgaaccaaa ctaaggtact acccttcaaa taggtgacat gcggatcggt agcacaatgg
      481 ttagtgcagt aatctcataa attaaaggtt gtgggttcga gtcccacccg atccattttt
      541 ttaattgaca tttacacaca ggggtactat aataccccat atagggtagt agtttaatgg
      601 taaaacagta gtctccaaaa ctaccattcc gggttcgagt cctggctacc ctgtaatttg
      661 ttagccctaa agtcctattg acttatccac aaacatacta atcattcaag gagtttaatt
      721 catgttagga tttattttct tttgtgtttt tattggcatt ttgcaatcgt gtagccgata
      781 agtaccattg gcaaatttgc aaatcaaggt ataatttggt tatgaatgaa cgagcacaaa
      841 acctacttaa catactcaat acagcactaa acacgggtga tgcccttgca ctggtaaaag
      901 cacgtaatta cttggcactt ttgtgtgaca tttacaccga ggacatttat ctagcaatgg
      961 acgcggttgc tagtgaatgg gcgcttgtgt gggggttaag attcgcgcat aacacggtgg
     1021 ttttgttcac ctcttactat gacctgtggc gttatttgca agtcaaggaa acaaacccca
     1081 ctgcatacct tgatgacaac tttgttacat acgttgccat gtgtgacata aatgaaagcg
     1141 aagtccatat tttacgagat aaacattttg caatagtatt ttaatactat tgcatttgca
     1201 caactaacga ggtatcatat aaacatgaaa gcacaaattc cacaattgct gttatattta
     1261 tggtaacaaa ttaaggagca gcacaatgca tcaaattaac ttaccgacaa acgagttttg
     1321 ggttgaatac aaaggcttta ccattattga agattggcaa agttggcgca ttttcaaggg
     1381 taaacaagtc ctttacactg taaaaacttt agatgaaggg cggcagtttg ttgataaggc
     1441 tttagaagcg gccgccttac agggtgatgg gcgcggttta ggttttgccg tgattattgc
     1501 agaacagctt actggtggat actggtttgg ggtgcagtat caccatactg ataaagtgca
     1561 aatgtttgaa tggcaagctg tgaccataca cagcgcaatg tatcccgatt ttagtcataa
     1621 ccttatggcg cacgttcaaa tatgaaatgc aacatgttcc taatccgacc tatggtacgt
     1681 ttttaggctg gttgtcacat aatcttaa
//
LOCUS       DAOPLS010000177         1707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_83486_length_1707_cov_5.525424, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000177 DAOPLS010000000
VERSION     DAOPLS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83486_length_1707_cov_5.525424"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKV52_03790"
     CDS             complement(<1..564)
                     /locus_tag="PKV52_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99363.1"
                     /translation="MDGIKPPIKKRTYYPASNQKEQFIKPASTQPQSKTKRSVNIKHF
                     FNKRPKSSRPIYKAPDKVSFSVKIPRFLQKLINVYMGLPFLLRIFALMMAITMFVIIV
                     LDVFYNKNQVDAYSLNKTDVLLKESSPIYGTSIVKDATGKYYKYNEGYKPGAEVSGLS
                     ASPKFSADFAVDPKEGVSVTDVNSNLTV"
     gene            complement(1201..1434)
                     /locus_tag="PKV52_03795"
     CDS             complement(1201..1434)
                     /locus_tag="PKV52_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99364.1"
                     /translation="MHNTHITNQAHQITFDSFGLLLADLWLTVCGARLLYHHVQLAYA
                     SRGYTKTQLICERFQASGICKYQFITTHNTTIF"
     gene            complement(1443..>1707)
                     /locus_tag="PKV52_03800"
     CDS             complement(1443..>1707)
                     /locus_tag="PKV52_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99365.1"
                     /translation="DNTTNAAVTYEMFRSEGGTLYRCGTGETAVTTSANTWQTVGING
                     NEATGCGFTSSSGGAYVIFKVNVKANSNANAYVSTLSFTTTGK"
BASE COUNT          476 a          321 c          337 g          573 t
ORIGIN      
        1 tactgttaag ttactattta catcagttac ggatacacct tcttttggat ctacagcaaa
       61 gtctgcacta aatttggggc tagcagaaag tccactcact tcagcgccag gtttatatcc
      121 ttcgttatat ttgtagtatt tacccgtagc atccttcacg atactagtgc cataaatggg
      181 tgaactttct ttaagcaaca catcggtttt gtttaaagag tacgcatcaa cttggttttt
      241 gttatagaaa acgtctagca ctataatcac aaacattgtt atagccatca ttaaagcaaa
      301 tatcctcaac aaaaatggta aacccatata aacattgatg agcttttgta aaaatcttgg
      361 aatcttaaca ctaaagctga ccttatcggg tgctttgtat ataggtcttg atgacttagg
      421 tcgcttatta aaaaagtgtt taatatttac agaacgttta gttttacttt gtggctgggt
      481 tgatgcaggc ttaataaact gctctttttg attactagca ggataatagg tgcgtttttt
      541 gattggtggt tttataccat ccatttttcc ttttttgtta ctccttaaag tacgtatata
      601 aagtatagca aaaatgctag tgttttgaaa caataatatc agcattttta cacgactcta
      661 acagatactc ttaataccga ttattactgc aaatggaatg tgtcgcacaa gtagttttat
      721 tttttagcat tctaaagagt tcttacatag aaatgttata tctaaaaagc ctgggggttg
      781 gcccaggctt ttttgttttg atgtagacga tctatttggc atcgcctgtg ttggtgttag
      841 ttttggtaag ctatttttgc ttactgtttg ctgtgtttag tgttcttgtg gctaatcact
      901 agccactata gcctaaccac tatttgtagt ttttattttt gtgtcctggg tttgtttttg
      961 gctgcttgaa gtccgcacaa cagcgaccaa ttttttgagg agagctaatc cccaagactt
     1021 tttgttttta gggtatgttt aagaatgggg caaaagaaca attaaaaccc ctttggcgta
     1081 ataacttgtc gcgagaccag ttattagttc atataacgcg cacgatttct cgttacccta
     1141 acgcgttttt cggcggcgat aaaccgccga aactgtgact aaaaagaata atatttgtgt
     1201 tcaaaaaatt gttgtattgt gggtggtaat gaattgatat ttacagatac cactcgcctg
     1261 aaaacgctca cagataagct gagttttggt gtatccgcga gacgcgtaag cgagctgtac
     1321 gtgatggtac aacaagcgcg caccgcaaac ggtaagccat aaatcagcca gcaataaacc
     1381 aaagctatca aatgtaattt gatgcgcttg gtttgtgatg tgagtgttat gcatattcat
     1441 cattatttac ccgttgtcgt aaagcttaat gttgatacat aggcattggc attactgttg
     1501 gcctttacgt tgaccttaaa gattacatag gccccgccag acgaacttgt aaagccacaa
     1561 ccagttgcct cgttaccatt aatacccact gtctgccacg tgttggcaga tgtcgtaacg
     1621 gctgtttcac cggtgccaca acgatacaac gtaccacctt cactgcggaa catctcatag
     1681 gtaacagcgg cattagtagt gttgtct
//
LOCUS       DAOPLS010000178         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_85312_length_1682_cov_3.934849, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000178 DAOPLS010000000
VERSION     DAOPLS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85312_length_1682_cov_3.934849"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..752
                     /locus_tag="PKV52_03805"
     CDS             21..752
                     /locus_tag="PKV52_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPD99366.1"
                     /translation="MSGHSKWATIKRQKGANDAKRGALFTRIGNQIAVAARAGTDPLT
                     NTSLAMVIEKAKAANMPMSNIERAIARVADKNAAQLTEVMYEGYGPGGVGILVECATD
                     NLNRTYPEVRHAFAKHGGNIAESGAVAFQFTRKGIIRMQATGDDAMLAALDAGAEDVN
                     EAGTEMLVYTEPKQLAKVRDTLKAKNFSIAEAELTYVPNNTIIIDNAETAAKIERLLD
                     ALDELQDVISTHVNFDVPDEVADKT"
     gene            749..1222
                     /gene="ruvC"
                     /locus_tag="PKV52_03810"
     CDS             749..1222
                     /gene="ruvC"
                     /locus_tag="PKV52_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPD99367.1"
                     /translation="MKMLGIDPGTGIVGFGVIETDRRKHKMLDAGVIRTPAHQADELR
                     LHTIYTSLQEIIQEHNPDVMVVERLFFARNVTTAMSVSQARGVILLAATEKGIPIAEY
                     TPLQIKQALTGYGRAEKQQIQEMVKVLLGLQQIPKPDDCADALAAALTHATAPLL"
     gene            1231..>1682
                     /locus_tag="PKV52_03815"
     CDS             1231..>1682
                     /locus_tag="PKV52_03815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HPD99368.1"
                     /translation="MIALLEGTIVEHIDDQVIITCGGVGYGALVTSEDYGRLPVGKSA
                     RLYIYEHIREQSHDLFGFIDISSKELFEILLSVNGVGPKMALAIMSVAPSTEVRKAIA
                     EGNMALLKAANGVGKRVAERIIVDLKEKVGYVSSDEGAYNGLNTLVSD"
BASE COUNT          554 a          302 c          391 g          435 t
ORIGIN      
        1 taattaaaag gactattgtc atgtcaggac attctaagtg ggctaccatc aagcgccaaa
       61 aaggtgcaaa cgacgccaag agaggtgcat tatttacccg tatcggaaac caaattgctg
      121 tagctgcaag ggctggtact gatcctttaa cgaatactag cctagcaatg gtgatagaaa
      181 aagccaaggc ggctaacatg cccatgagta acatagaacg agcaatcgcg cgagttgctg
      241 ataagaatgc tgcccaattg actgaagtaa tgtacgaagg ctatggtccg ggtggagtag
      301 gcattttggt agagtgtgca acagataacc ttaatcgtac ctatccagaa gtgcgccacg
      361 cttttgcaaa acatggtggt aatattgctg aatctggagc agtcgcattt caatttacca
      421 gaaaaggcat tattcgtatg caagctactg gagatgatgc tatgctagca gcgctagatg
      481 ctggtgcaga agatgtcaat gaagctggta ctgaaatgtt ggtttataca gaaccaaaac
      541 agcttgctaa agttcgtgat accttaaaag caaaaaattt ttctatagca gaagcagagc
      601 taacgtatgt acctaataat acaatcatca tagataatgc agaaacggct gcaaaaatcg
      661 aacgattact agacgcacta gatgaattac aagatgtcat tagtacacat gttaactttg
      721 atgtacccga tgaagtagca gataaaacat gaagatgctt ggcattgatc ctggtacagg
      781 cattgttggt tttggtgtca ttgaaacaga tcgtcgcaaa cataaaatgc tcgatgccgg
      841 tgtaattaga acacccgcac atcaggccga tgagttacgt ctacacacaa tatacacatc
      901 cttacaagaa ataatacaag agcacaaccc agatgtcatg gttgttgagc gcttattttt
      961 tgcacgtaat gttactactg ctatgagtgt gagccaagca aggggagtga ttttacttgc
     1021 ggctacagaa aaaggcatac caatagccga atacacacct ttgcaaataa agcaggcact
     1081 aacaggctat ggtagggcag aaaaacagca aatacaagaa atggtaaaag tactccttgg
     1141 tctacaacaa attccaaagc ctgacgactg tgctgatgct ttagctgcag cacttaccca
     1201 cgccacagcg cctctgttat aataaagtat atgattgcac tactagaagg tacgatagtt
     1261 gaacatattg atgaccaggt gattattacc tgtggaggtg taggctatgg tgctttggta
     1321 acatcagaag attatgggag gttacctgta ggcaagtcag cacgtttgta tatttatgaa
     1381 cacattcgcg agcagagtca tgatttattt ggttttattg atatatcatc aaaagaatta
     1441 tttgaaatac tacttagtgt gaacggggtg ggtcctaaaa tggcgttagc tatcatgagt
     1501 gttgcgccaa gtacagaagt tagaaaagcc attgcagaag gtaatatggc gttattaaaa
     1561 gctgccaacg gtgttggtaa acgggtagct gaacgtataa tcgtagattt aaaagaaaaa
     1621 gtaggctatg tcagtagtga tgaaggtgca tataacggct taaatacttt agtaagtgac
     1681 ga
//
LOCUS       DAOPLS010000179         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_85364_length_1682_cov_2.310387, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000179 DAOPLS010000000
VERSION     DAOPLS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85364_length_1682_cov_2.310387"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PKV52_03820"
     CDS             <1..549
                     /locus_tag="PKV52_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPD99369.1"
                     /translation="EERLYTIATIQGTDSVKLELLTDIERCVATREVYLVWSLLKKDK
                     NDWVLQKATELGVHKFIPMIAQRSEKTELNIERAKRIIIEAAEQCGRADIPELREPIS
                     LHEALEEYKDLPLCIAEQHDDQNTIPTDTKKVGILIGPEGGWSDEEKIYFKELALPHV
                     SLSRFTLRAETAAITAVSRLLS"
     gene            642..1208
                     /locus_tag="PKV52_03825"
     CDS             642..1208
                     /locus_tag="PKV52_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99370.1"
                     /translation="MSFIDHFRHPINSDEIRFITEEQQRLEQAHVALFTEAQHLRDIC
                     DYMLAGRCNALVPTYDRFAEYNNNAPDFSEEKYRITCFRPNDSLRLVQSKGGRYVDCV
                     ISDSQVSITHEERAANGEHKISSQFTACKPAPHSHSTLELAALSEELLALQENLLIAA
                     GYRELTMNPNARERLAKTAVEHVVSSTF"
     gene            1234..>1682
                     /locus_tag="PKV52_03830"
     CDS             1234..>1682
                     /locus_tag="PKV52_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99371.1"
                     /translation="MAYYRNTRYTLGQTKPYTISRSKIELFTQCQRCFWLEARLGIKR
                     PSSPPFQINKAIDELFKKEFDFYREKQQPHPIMVQNKLAAVPFKHKDVVLWQDTFKGI
                     RTLHTGTNLEVFGGIDDVWIDANEQLIVVDYKATAKAKEVTLDADWQ"
BASE COUNT          563 a          335 c          361 g          423 t
ORIGIN      
        1 gaagagcgtt tatatacaat tgccacaata caaggtaccg attctgttaa actagaactt
       61 cttacagata tagaacgttg tgtagcgacc cgtgaagtat acttagtgtg gtcactactt
      121 aaaaaagata aaaatgattg ggtattgcaa aaagccacag agcttggtgt gcataagttt
      181 ataccaatga ttgcccaaag aagtgaaaaa acagagctta atatagaacg agccaaacga
      241 attatcatcg aagcagccga acaatgtggc agagcagaca ttccagagtt gcgagaacca
      301 attagcctac acgaagcatt agaagaatat aaagacttgc cgctatgtat tgctgaacag
      361 cacgatgatc aaaacaccat accgacagat acaaaaaaag taggcatctt gatagggccc
      421 gagggtggct ggtcagatga agaaaagatc tattttaaag aattagcctt accccatgtg
      481 tcgttatctc gttttacttt gcgggcagaa acagctgcaa tcactgccgt aagcagacta
      541 ctgagctagt tgccaaaaaa tatacttgat tttgtaatac gttaggcgta taccatatat
      601 atcttatgtt taatgagtat aaataaggta catatacgca aatgtcattt attgatcatt
      661 ttcgtcaccc cataaatagt gatgaaatcc ggtttataac agaagagcag cagcgcttag
      721 agcaagccca cgttgcttta tttactgaag cgcaacacct aagagatata tgtgactaca
      781 tgcttgcagg ccgttgtaat gctctggttc ctacttatga tagattcgca gaatataaca
      841 ataatgcacc cgatttcagt gaagaaaaat atcgcatcac gtgttttcgg ccaaacgatt
      901 ctttgcgtct cgtacaaagc aaggggggca ggtatgtaga ctgtgtgata tctgatagcc
      961 aggtaagcat tactcatgaa gaacgagccg caaatggtga gcacaaaatt tcttcgcaat
     1021 ttacagcgtg caaacctgca ccacatagcc acagtacttt agaactagca gcgctttctg
     1081 aagagctgct cgcgctacaa gagaatttgc ttatagccgc tggatacaga gagctgacaa
     1141 tgaacccgaa tgctcgtgaa cgattagcaa agacggctgt cgaacacgta gtgtctagca
     1201 cgttttagcc tgctgtctgt tacaattggt tgaatggcat attacagaaa cacgcgttac
     1261 acacttgggc aaacgaaacc atatacaatt agtcgtagta aaatagaact ctttacacag
     1321 tgccagcgtt gtttttggct cgaggcgagg cttggtatta aaagacccag cagcccaccg
     1381 ttccaaataa ataaggcaat agacgaactc ttcaaaaaag aatttgattt ttaccgcgaa
     1441 aaacaacaac ctcaccctat tatggtacaa aataagcttg cagctgtacc atttaaacat
     1501 aaagatgtgg tgctatggca agatacgttt aaaggaatcc gtacactgca cacgggtact
     1561 aacctagaag tgtttggtgg catagatgac gtttggatag acgcaaatga gcagcttatt
     1621 gtggtagatt ataaggccac cgcaaaagcc aaagaagtga cgctagacgc cgattggcaa
     1681 aa
//
LOCUS       DAOPLS010000180         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_86287_length_1670_cov_3.272446, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000180 DAOPLS010000000
VERSION     DAOPLS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86287_length_1670_cov_3.272446"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKV52_03835"
     CDS             complement(<1..384)
                     /locus_tag="PKV52_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPD99372.1"
                     /translation="MDDVTDTVLRQIVADCSPPDLFFTEFVNVDGLQSKGRDKLLHRL
                     DYTAKERPLIAQIWGKNPENYEKTAKELVAMGFAGVDINMGCPDKSITKNGCCSALIN
                     NRELAVSIIAAVKRGVAGRIPVSVKT"
     gene            complement(449..829)
                     /locus_tag="PKV52_03840"
     CDS             complement(449..829)
                     /locus_tag="PKV52_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99373.1"
                     /translation="MDSANFPNYDPSAYEKLPIDRRKSKKWTDDGRAALGMKPLYHDD
                     EKIEFVSAEAVNLSNKIFYSDLQKPLIVRKLEDMILQAYVNQKITEEEASGLVCYIEE
                     VLVYQENQPPARDSDARKRQLNDS"
     gene            complement(862..>1670)
                     /locus_tag="PKV52_03845"
     CDS             complement(862..>1670)
                     /locus_tag="PKV52_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD99374.1"
                     /translation="DATIILPQSFGQQQNGIPSGQVTVLYNENSQQAGSALSSILKAQ
                     MDELNKQFVDTYVPFTVVQESTKAQGLSQFDYTFAGLVGFAIISLGIFGPTNVFPELK
                     KQGVLRRLHTTPLKVRHYFIANVGSQGLIGMLSIATMFIFSLLVFDLQMKGSYVTFAA
                     FVALSIVMIYGIGLAIGGWAKNERQAAPLSNLVTFPMIFLSGTFFPRFLMPEWLQSVT
                     NLLPLTPVIDGVRLIVTEGKGMLEILPQVGLVAVWALVIYLIAFRVFRWE"
BASE COUNT          492 a          362 c          319 g          497 t
ORIGIN      
        1 ggtttttaca cttactggta tacggccagc cacaccacgc ttaacggcgg ctatgatgct
       61 aactgcaagc tcgcggttat taatgagcgc cgaacaacag ccgttttttg tgattgattt
      121 atctgggcaa cccatattaa tatcaacacc agcaaagccc atagcaacaa gttctttggc
      181 ggttttctcg taattttctg gatttttgcc ccatatttga gcaattaacg ggcgttcttt
      241 agcagtgtag tctaacctgt gcagcagttt gtcacgcccc ttactttgta agccgtctac
      301 attgacgaat tcagtaaaaa ataaatctgg tggtgaacaa tctgcaacaa tctggcgtaa
      361 aactgtatct gtgacgtcat ccattggtgc caaaacaaaa aaaggcctct ttagactatg
      421 tagtgaaaac atattttgtt attatacgct acgaatcatt aagttggcgc ttacgcgcat
      481 cgctatctct agctggtggc tggttttctt ggtacactag tacctcttct atgtagcaga
      541 ctaagccaga agcttcttct tcagtaattt tttgatttac ataagcttgt aaaatcatat
      601 cttctagctt gcgtactatg agtggttttt gtaaatcgct gtagaatatt ttattagaca
      661 aatttaccgc ttcagcagac acaaactcta ttttttcatc atcgtgataa aggggtttca
      721 tacctaacgc cgcccgacca tcatcagtcc attttttgct ctttctgcga tcgattggta
      781 atttttcata agcagaagga tcataatttg gaaaattagc gctatccata aatatattat
      841 agcatgttta tgcttatatg tctattccca gcggaagact ctaaacgcaa taaggtaaat
      901 tacgagtgcc catacggcca ctagccctac ctgtgggaga atttctagca tacccttgcc
      961 ttctgtcaca ataagtcgca ccccatcaat aacgggtgtt agtggtaata aattggtaac
     1021 agattgtagc cattcaggca tgagaaacct tggaaaaaac gtgccagaca aaaatatcat
     1081 tggaaatgtc actaaattac taagcggtgc agcttggcgt tcgttttttg cccagccacc
     1141 aatagccaag ccaatgccat atatcatgac aatgctaagc gccacaaacg ctgcgaatgt
     1201 tacatagcta cctttcatct gtaaatcaaa aacaagtaga gaaaaaataa acatcgtagc
     1261 gatagataac atcccaatca ggccctgtga cccaacgttt gctataaaat agtgcctcac
     1321 tttcaagggt gttgtatgta agcgacgcaa tacgccttgt tttttaagct ctggaaagac
     1381 attggtcggg ccaaaaatgc caaggctaat aattgcaaag cccactaaac cagcaaaggt
     1441 gtaatcaaat tgcgacagac cttgtgcctt ggtagattct tgcacaacag taaaaggtac
     1501 gtacgtatca acaaactgtt tattcaactc gtccatctgg gcttttagta tagaagacaa
     1561 cgcactacca gcttgctgac tattttcatt gtataaaacc gttacttgcc ctgatggaat
     1621 accattttgt tgttggccaa aactctgggg cagaatgatt gttgcatcta
//
LOCUS       DAOPLS010000181         1669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_86387_length_1669_cov_2.781289, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000181 DAOPLS010000000
VERSION     DAOPLS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86387_length_1669_cov_2.781289"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PKV52_03850"
     CDS             <1..1119
                     /locus_tag="PKV52_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013999220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPD99375.1"
                     /translation="RLGELIDLFSNITFDKDHSSRDLLGRIYEYFMGMFADAEGKRGG
                     QFYTPRSIVRLLVNMLQPKPGMRIYDPCCGSGGMFVQSEEFIEDNAHSTNVAVYGQES
                     NQTTWRLAKMNMAIRGIDAQIEYGDTLHNDKLPDLKADIVIANPPFNVSDWGGETLRN
                     DARWAYGVPPTGNANFAWVQHFVHHLAPNGTAGFVLANGSMSSQSSGEGEIRKKLVEA
                     GIVDAIVTLPSQLFFNTGIPACLWFISRDRANRKDKVLFIDGRNLGKMINRRNRELAE
                     EEISKVADTYQNWREQNENYHDEPGFCKSANLAEVKEHDYVLTPGRYVGSEEVEEDDE
                     AFEDKMAKLTKDLSDQFVKSHDLEAKIKENLKKIGYNL"
     gene            1137..>1669
                     /locus_tag="PKV52_03855"
     CDS             1137..>1669
                     /locus_tag="PKV52_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HPD99376.1"
                     /translation="MDESGDHSLSKINEDFPIFVLSGLLFEESSYLKISKAVDSFKVK
                     YFNTKSVILHSRDIRKCDGPFKILFDLDLKMRFYKDLDDILKTADFKIISSAIIKNKH
                     IAKYGKLADDPYEISLTFVLERTLFEADDSKNPNSIDVIIEGRGSKEDIQLASRYNEL
                     LYRGTGMIMPDRLLNAY"
BASE COUNT          562 a          289 c          362 g          456 t
ORIGIN      
        1 cgtttgggtg agttaattga tctatttagt aatattacat tcgataaaga ccatagcagc
       61 cgcgatttgt taggtcgcat ctatgaatat ttcatgggta tgttcgccga tgccgagggc
      121 aagcgtggcg ggcaattcta tacaccgcgt agcatagtta gattactagt aaatatgcta
      181 cagccaaaac ccggtatgcg tatctatgac ccgtgctgtg gcagtggcgg tatgtttgta
      241 caatcagagg aattcataga agataacgct catagtacta acgttgctgt ctatggccaa
      301 gaatccaacc aaactacttg gcggctagca aaaatgaaca tggctattcg tggcatagat
      361 gctcagattg agtatggcga tactttgcat aacgataaac tgccagatct taaagcggat
      421 atagtaattg caaatccacc gttcaatgtt agtgattggg gtggagaaac tttacgtaat
      481 gatgcccgct gggcttatgg cgtaccacca acaggaaacg caaactttgc atgggtacaa
      541 cattttgtgc atcatcttgc accaaatggc acagcaggct ttgttctcgc caatggtagc
      601 atgagcagcc aaagctcagg cgaaggtgaa atccgcaaaa aactagtaga agctggcatt
      661 gtggatgcaa ttgtcacttt gccaagccag ttattcttta atactggtat acccgcttgc
      721 ttatggttta tttcgcgcga tcgtgcaaac cgcaaagaca aagttttgtt tattgatggc
      781 cgtaatttgg gtaaaatgat taaccgccgt aaccgcgaat tagcagaaga agaaattagt
      841 aaagttgcag atacatatca aaattggcgc gaacaaaatg agaattatca tgatgagcca
      901 ggtttttgta aatcagctaa tctagcagaa gtaaaagagc acgattatgt gctaacgccc
      961 ggccgctatg ttggcagtga agaagtagaa gaagacgacg aagcatttga agacaaaatg
     1021 gccaagctta ccaaagattt atcagatcag tttgttaaaa gccacgatct agaagccaaa
     1081 atcaaagaaa acctaaaaaa gattggctac aacttgtgaa aaaatacttc ttatttatgg
     1141 acgaatccgg cgaccatagc ttaagcaaaa taaatgaaga tttccctata ttcgtgttaa
     1201 gcggattgtt atttgaagaa tcatcatacc ttaaaataag caaagcagta gattcattta
     1261 aggtaaaata ctttaatact aaaagtgtaa tattgcatag cagagatatt cgaaaatgtg
     1321 atggaccgtt taagatattg tttgatcttg atttaaaaat gcggttttac aaagatcttg
     1381 atgatatatt aaaaacagcg gattttaaaa taataagctc ggcgataatt aagaataaac
     1441 atattgccaa gtatggtaaa ttagctgatg acccttatga aataagttta acatttgttc
     1501 ttgagcgcac actttttgaa gcagatgaca gcaaaaatcc aaatagtata gatgtaataa
     1561 ttgaaggcag aggctctaaa gaggatatac agctagctag tcgctataac gaactgctat
     1621 accgtggtac aggtatgata atgcctgata gactcttaaa tgcgtacag
//
LOCUS       DAOPLS010000182         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_88970_length_1635_cov_3.640506, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000182 DAOPLS010000000
VERSION     DAOPLS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88970_length_1635_cov_3.640506"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..648)
                     /locus_tag="PKV52_03860"
     CDS             complement(151..648)
                     /locus_tag="PKV52_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HPD99377.1"
                     /translation="MALFSFDLESEYDKSEMNNVVDQAQREIANRYDFKNTTAQLSWK
                     NDDKQALLINGDTQFQLDAIIEIIRKKLALRGQSQKCLDVSAEPTTNNLRMSLTVPLV
                     RGLDQAKAKAITSLVREHYPKVKTQIQGETVRIMSAKKDELQNVITLLKTHEFDFPII
                     FTNYR"
     gene            complement(666..875)
                     /locus_tag="PKV52_03865"
     CDS             complement(666..875)
                     /locus_tag="PKV52_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99378.1"
                     /translation="MSNQKPRIGDRVSASHAAGNEFCANATCRTYEGFITDIVQDIIT
                     IYDGQHHVRIKNTGAWVLEIYDEVT"
     gene            952..>1635
                     /locus_tag="PKV52_03870"
     CDS             952..>1635
                     /locus_tag="PKV52_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99379.1"
                     /translation="MSYTFQEVQSAKAKLQQEFNQLSDKTAIVRSPVFKQLYAELPNK
                     NSQQKPLFGAEVNALKKEVTSWFSTEAVQDNRQPIDVTAPFAPNTTKRPQTFTSSVGS
                     EHPLMSEITYILDIFYRMGFTAMESRQVDDDYHMFESLNFPEGHPARDDYDTFMTTEG
                     LIAPAHTSTMQNRALIQYKNNLMNGEPIAVVIPDRVFRNEDLDATHEHAFYQLEGIYV
                     DKGINVGNLI"
BASE COUNT          483 a          343 c          315 g          494 t
ORIGIN      
        1 accagcctat cgccgcaaaa caactatccc acaaccactg atgagggtaa aggccaggtg
       61 atggcaccga atgtgtaccc ctatcattgg ctctaagaac gtcttcgcat gtgacagcca
      121 acgtattcat atactattag tatagttcta ttatcgataa ttggtaaata taatcggaaa
      181 atcaaattcg tgggttttta atagcgttat cacgttttgg agttcatctt tttttgcact
      241 cataatacga actgtttcgc cctgtatctg ggtttttact tttggataat gctctctcac
      301 gagtgaagtg atagctttag ctttggcttg gtctagacca cgtaccaagg gcaccgttag
      361 actcattcgc aaattattgg tggttggttc agcacttaca tctaaacatt tttggctttg
      421 acctcgaagg gcaagttttt tacggataat ttctataatt gcatcgagct gaaattgggt
      481 atcaccatta ataagtagtg cctgtttatc atcgtttttc cagcttaact gcgcagtggt
      541 atttttaaaa tcatacctat ttgcaatctc gcgctgggct tggtctacga cattgttcat
      601 ttctgattta tcgtattcac tttcaaggtc aaaactaaat agtgccatag tacatttctt
      661 tcgttttatg ttacttcatc gtatatttct aagacccacg caccggtatt ttttatccgc
      721 acatggtgct gtccatcata tattgttatg atatcttgca caatatctgt tatgaaccct
      781 tcgtacgtgc gacatgtggc atttgcgcag aactcgttac cagctgcatg gctggcagac
      841 acccgatcgc caatacgtgg cttttggttg gacatggtaa tttgaatata tatgtctcgc
      901 taaaaatgtc aatagcatgt ctatctgtac acgtagtata atgaaagcca tatgtcgtat
      961 acattccaag aagtacagtc tgcaaaagct aaattgcagc aagaatttaa ccagctcagt
     1021 gataaaacag ctatagtgcg cagccctgtg tttaagcagc tctatgcaga attgccaaac
     1081 aaaaatagtc agcaaaaacc tttatttggc gcagaagtaa atgcactaaa aaaggaagtt
     1141 acatcgtggt tttctacaga agcagtacaa gacaaccgtc aaccaattga cgtcactgcg
     1201 ccgtttgcac ccaataccac aaaaagacca cagacgttta cttctagtgt agggagtgag
     1261 catcctttga tgtcagaaat tacatatatc ttagatattt tttaccggat gggttttact
     1321 gctatggaat cgcgccaagt tgatgatgac taccatatgt ttgaaagtct taattttcca
     1381 gaaggccacc cagcccgcga cgattacgac acctttatga ccacagaagg tcttattgcg
     1441 ccagcacata cgagcaccat gcaaaatagg gcgcttatac aatacaaaaa taatttaatg
     1501 aacggcgaac caatcgctgt tgtaatccca gatagagtat ttagaaacga agacttagac
     1561 gccacacatg aacatgcatt ctatcagcta gaaggcatct atgtagataa aggcataaat
     1621 gtaggtaacc taatc
//
LOCUS       DAOPLS010000183         1632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_89250_length_1632_cov_2.529486, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000183 DAOPLS010000000
VERSION     DAOPLS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89250_length_1632_cov_2.529486"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PKV52_03875"
     CDS             complement(<1..1336)
                     /locus_tag="PKV52_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99380.1"
                     /translation="MNGASIIKDSVRVIPKNISPTSNMMLYMVQTHDIIEPWNGNQVK
                     RDEDLSKFWKEEPFLESAITSLAMTRAALSWELSGPPRTVKNTQIMLNSADFGQGWQH
                     LMMLVNTDLLTSDNGAFIEIIREKATRGRKPESMPVLGLAHLPAIRCFRTGNPMQPVI
                     YIDRDNKEHKLNWYQVITLAENPIPESETGRQLCFVSRVLGFAQMIKDVEQHHREKLS
                     GRFSKAIHVVSGVAQGEIETIKQVGEIDNNNRGMYRYGQPVILTTIDPNARVTKETIE
                     LASLPDGFDFDQLMNWYITLLALASGSDYQEFAPLSNGNLGTASQSDTLHRKAQRKGT
                     QLFIKQIETALHQAKVIPATVTFRFKQQDAAAELEQADIEKTRADTRSIQITSGEITP
                     AVARQIAVDKGDLRQQYLLLLGEQDATPNVTVSDDEDVADELLNPPAANTNVP"
     gene            1394..>1632
                     /locus_tag="PKV52_03880"
     CDS             1394..>1632
                     /locus_tag="PKV52_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endoglucanase"
                     /protein_id="HPD99381.1"
                     /translation="MRDTLRDILTVIALIVIADIVFGFGLTDTFFAKRGDPLTPVMLI
                     MTATPDQVVVPPTSTPLPTLTPLPTSTPLPTLTPTP"
BASE COUNT          441 a          354 c          311 g          526 t
ORIGIN      
        1 gtggtacatt agtattagct gctggcggat tcaataattc atctgcaaca tcttcgtcgt
       61 ctgacaccgt tacatttggc gtagcatctt gttcgccaag taacaacaga tattgttgtc
      121 gtaaatcacc tttatcaaca gcaatttgac gtgcaacagc gggtgtaatt tcaccactag
      181 taatttgaat actacgtgta tcagcacgag ttttctcaat atcggcttgt tctaattccg
      241 cagcagcgtc ttgttgctta aaacgaaatg ttacagttgc cggaattact tttgcttgat
      301 gtaacgcagt ttcaatctgt ttgataaaaa gctgtgtccc tttacgttgt gccttgcgat
      361 gcaacgtatc agactgtgaa gctgtgccta gattaccatt acttaatggc gcaaattcct
      421 gatagtcaga gccagatgct aatgccaaaa gtgtaatata ccagttcatt agttgatcga
      481 aatcaaaacc gtctggtaat gaagctaatt caatagtttc ttttgtaact ctggcgtttg
      541 gatcaatagt tgttaagatt accggttgtc cataacgata cataccgcga ttgttgttat
      601 caatttcacc aacttgtttt atagtttcaa tttcgccttg tgcaacgccg ctaacaacat
      661 gtattgcttt actaaaacga ccagataact tttcgcggtg atgttgttca acatctttga
      721 tcatttgtgc aaagccaagt acgcggctta caaaacacaa ttggcggcct gtttcagatt
      781 caggtatcgg attttcagct aacgtaataa cctgatacca gtttaattta tgttctttgt
      841 tatcacggtc tatgtagata actggctgca taggattacc cgtgcggaaa cagcgaattg
      901 ctggtaaatg tgccaaaccc aataccggca ttgattcagg tttgcgccca cgtgttgctt
      961 tttctcgtat gatctcgata aatgccccat tgtcggaagt taataagtca gtatttacca
     1021 gcatcatcaa gtgttgccaa ccttgcccaa aatcggcact atttagcatt atttgtgtat
     1081 ttttaacggt gcgtggtggg ccagacaact cccatgacaa tgcggcgcga gtcattgcga
     1141 gacttgtaat agcagattcc aagaacggtt cttctttcca aaacttactt aaatcttcat
     1201 cgcgttttac ctgattgccg ttccagggtt caattatgtc atgcgtttgt accatgtaca
     1261 acatcatgtt tgatgttgga cttatatttt ttggtataac acgcactgag tcttttataa
     1321 tacttgcgcc attcatgcaa taattgtatc aaactggctt gacatagcca tgtaaacatg
     1381 ttatagtcat gaaatgagag acacgcttag agatatactt accgtaattg ccttaattgt
     1441 aattgcagac atcgtttttg ggtttggact tacagacact ttttttgcta aacggggcga
     1501 tcctttgaca cctgtcatgt taattatgac agcaacaccc gatcaagttg ttgtaccacc
     1561 aacaagtacc cccttaccga ctttaacacc gttgcccaca agtacccctt tgccaacact
     1621 aaccccaaca cc
//
LOCUS       DAOPLS010000184         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_89615_length_1627_cov_3.765267, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000184 DAOPLS010000000
VERSION     DAOPLS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89615_length_1627_cov_3.765267"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKV52_03885"
     CDS             complement(<1..427)
                     /locus_tag="PKV52_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD99382.1"
                     /translation="MDRRNVSKKYSADVIVVGSGAGGSVAALTLADAGKRVIIVEKEA
                     VGGDCANYSCIPTKALLETAAMQKMITQAPAHGIKIGNVKLSYEAAIHSVNKSIHSTG
                     VLHPGHSPYDNANIQLVRGQAHCLDKHTISVDLTRYTAAN"
     gene            complement(439..708)
                     /locus_tag="PKV52_03890"
     CDS             complement(439..708)
                     /locus_tag="PKV52_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99383.1"
                     /translation="MTHVVKASGAIQNYSAEKHRRSIQRSLYAVHVKAAVAHEITDKI
                     LHACHSWRENKAEVTTLDLKTFTIKQLTHYDKDAAMVYKKHKDIW"
     gene            complement(705..1397)
                     /locus_tag="PKV52_03895"
     CDS             complement(705..1397)
                     /locus_tag="PKV52_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99384.1"
                     /translation="MNDSSLMLIGLMIGLTIGMSASSVGTYTSMMTSLLGHKKAQWEV
                     ITLAMLVLFYIFMLVIIASLTLATIITFFSPSYLDAMYIAGPIIGITVGLVYVRHYFW
                     DHPIVRITHHKTAAHKHTTKYSGYLQPLVVAATLLYVTGLPIIVSMLLLTLLAQLSGG
                     SMITWSVSLAFGIIVPLYGVLSLIASKTKVSRVIAWKESVKTTMALYSGLAIILLSWF
                     VLYAALIQGGNT"
     gene            complement(1394..>1627)
                     /locus_tag="PKV52_03900"
     CDS             complement(1394..>1627)
                     /locus_tag="PKV52_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99385.1"
                     /translation="AWYIASSTVAAQGVAARAIIAAALSIVGIILVYRFARKNPILAV
                     VLYCLVIFIILFTSVILIISAAQNIVSTAGVQI"
BASE COUNT          495 a          333 c          327 g          472 t
ORIGIN      
        1 tattggcagc agtgtagcga gttaaatcta cacttatcgt gtgtttgtct aaacaatggg
       61 cttggccacg tacaagctgt atatttgcat tgtcatatgg gctatgaccc ggatgtagca
      121 cgcctgtact atgaatggat ttattaacag aatgaattgc agcttcgtaa ctgagtttta
      181 cattcccaat ctttatgcca tgggcaggtg cctgtgtaat cattttttgc atagctgctg
      241 tttctaatag tgcttttgta ggaatacaag aatagttggc acaatcacca ccgacagctt
      301 ctttttctac tatgatgacg cgtttgccag catcggcaag ggttaaagct gcaacagacc
      361 caccggctcc gctaccaact acgatgacat cggcactata ttttttactt acgttacgtc
      421 tatccatatt gcttaacctt accatatatc cttatgcttt ttgtagacca ttgcagcatc
      481 tttatcatag tgagtgagct gttttatagt gaaagtttta agatctaggg tagtgacttc
      541 tgctttgttt tctcgccacg aatgacaagc gtgtaaaatt ttgtcggtaa tctcatgagc
      601 aactgcagct tttacgtgta ccgcatataa gctgcgctga atgctacggc gatgtttttc
      661 tgccgagtaa ttttgaatgg ccccagatgc tttaacgaca tgtgtcatgt gttgccacct
      721 tgtattaatg cagcatacaa aacgaaccat gataatagaa taattgctaa cccactatat
      781 aacgccattg tggttttgac actttctttc catgcaataa ctcgtgagac tttggtttta
      841 cttgctataa ggcttagtac accatataac ggaacgatta taccaaacgc caaagagaca
      901 ctccatgtaa tcatagagcc accagataat tgagcgagca gtgttagtaa aagcatactt
      961 acaataatag gcaggccggt cacgtacaaa agtgtcgcag cgactactaa tggctgcagg
     1021 tacccagaat attttgtagt atgtttatgt gcagcagttt tatggtgtgt tatgcgcaca
     1081 atagggtggt cccaaaaata gtggcgcaca tatacaagac caacggttat cccaataatt
     1141 gggccagcta tatacatggc gtctaaatac gaaggactaa aaaatgtaat tatagttgca
     1201 agggtaagcg aagcgatgat aacaagcata aatatgtaga agagaactaa catagcaagt
     1261 gtaataactt cccactgggc ttttttatgt cctagaagtg atgtcatcat agaagtgtag
     1321 gtaccaacag acgacgcgct catgccaata gtaagaccaa tcatcagccc aattaacata
     1381 agactagaat cattcatatt tgaaccccag cagtactgac tatattttga gcagcgctaa
     1441 taataagaat tacgctcgta aaaagtatta taaagataac aaggcaatac aaaacgacag
     1501 ccagaatagg attttttctg gcgaaccgat ataccaatat aattccaaca atactcaatg
     1561 cagcagcaat aattgcgcgc gctgcaactc cttgtgctgc tactgtactg ctggcgatat
     1621 accaagc
//
LOCUS       DAOPLS010000185         1623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_89950_length_1623_cov_3.981505, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000185 DAOPLS010000000
VERSION     DAOPLS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89950_length_1623_cov_3.981505"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKV52_03905"
     CDS             <1..274
                     /locus_tag="PKV52_03905"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPD99386.1"
                     /translation="KRVAVISTFYFLRKGIVEESLYICELLVDEKHDLLQKAVGWCLR
                     EIGKIDEPVLLTFLEQHAATMPRTMLRYSLEKLPQVRRLYYMSLKK"
     gene            326..1267
                     /locus_tag="PKV52_03910"
     CDS             326..1267
                     /locus_tag="PKV52_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPD99387.1"
                     /translation="MVQSNTNTTTNWRLIVGAIVSVMAVGFVYFQRATIKRSIEALQS
                     ADWVYVAVAIAVYSITIFAAAAVIYNLRLIYTLRYKYILLVQISTLFLGRITPASVGG
                     LAAMGRLLFTQGHTVVQSGTVVGAGGIATFIGNVILTLGALLLSLNNVKLSTVSIPSF
                     IIYVLVAIVVVSVILLLIPVVRTRIVHTVKDVVNTLKSYSHRKTSIVAAISFGSLVTL
                     CFALTLMLAAKSLGIELSLFAAIITVSLGSLGVAVTPLPGGVVGAEAALAATMVQFGV
                     ASDTALAIAFVYRFVIFWLPLIPGYIASQYALKKQLL"
BASE COUNT          439 a          315 c          348 g          521 t
ORIGIN      
        1 aaagcgggta gctgttatta gtacgtttta ttttttgcgc aaaggtattg tagaagagtc
       61 gctctatata tgtgagctac tggtggatga aaagcatgat ttgttgcaaa aagccgttgg
      121 ttggtgcttg cgtgaaatag gcaagataga tgagcctgtc ttgctgacat ttcttgaaca
      181 acatgccgcc acgatgccgc gcacaatgct aaggtactca ttagaaaaac taccacaagt
      241 acgtcggttg tattacatgt ccttaaaaaa ataattaacc ctagtttttt tacccatagg
      301 gtatacttat acgtagtagg tatatatggt acaaagtaat acaaatacaa caactaattg
      361 gcggttaata gtcggcgcaa tagtttctgt tatggcggtt ggttttgtgt attttcaacg
      421 cgctactata aaaagatcaa tagaagcact gcaatctgca gactgggttt atgttgccgt
      481 tgctattgct gtatattcaa taactatttt tgcagcagca gcagtaatat ataacttacg
      541 actcatttat acgttgcgct acaaatatat cttgctcgtt caaataagta cgttattttt
      601 gggtcgtata acaccggcaa gtgttggtgg cttagcagcc atgggccggt tgttatttac
      661 acaagggcac acagttgtgc agtctggtac agtagttgga gccggtggta tcgccacgtt
      721 tataggtaat gtcatactta cgcttggggc tttattgtta tctttaaata acgtcaaact
      781 tagcacagtc agtatcccta gcttcatcat atatgtattg gttgcaatag ttgttgtgtc
      841 tgttatttta ctacttatac ccgttgtccg tacccgaatc gtgcacacgg taaaagatgt
      901 tgtaaatacc ttaaaaagtt atagccacag aaagacatct attgttgcag ccatttcttt
      961 tgggtcactt gttacactgt gttttgcact aacgctgatg ttagcagcga aatcattagg
     1021 gatagagtta agcctttttg cagcgattat tacggttagc cttggtagtt taggagtcgc
     1081 cgttacgccc ttaccggggg gcgttgtcgg agcagaagct gcactcgctg ctacaatggt
     1141 gcaatttggc gtagcaagcg atacagcgct ggctattgca tttgtgtata gatttgttat
     1201 cttttggcta ccgcttatac ccggctacat cgccagtcaa tatgctctca aaaaacaact
     1261 cctctaagtt agatatttga tgttagattt tggatatttg attctcgaat ctagaatata
     1321 tttttccatt cctactttgc caccaaattt tatatttact ttttctaccg gctaaaaagt
     1381 aacaaaaagc ctcggccgac tgccacatca gttcttagct gtagttaaaa cctaccatgg
     1441 cgcaaaaaga acttgccggc tttcttatac ttcttaatga agagccggct tcaacagctt
     1501 tttgccttgg ccaggatatg ttttactaca tctaaatcaa cagtgtggca gaggccagtg
     1561 cgttccttgg gaacgcacat aaagaaggta tcttcatcta ccaatcccta agggattggc
     1621 ggg
//
LOCUS       DAOPLS010000186         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_90663_length_1615_cov_1.895513, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000186 DAOPLS010000000
VERSION     DAOPLS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90663_length_1615_cov_1.895513"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1615)
                     /locus_tag="PKV52_03915"
     CDS             complement(<1..>1615)
                     /locus_tag="PKV52_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99388.1"
                     /translation="FTVDPLSGGVIFEDYGQQLQDQLRTTVGYYVDDFFETADSINAQ
                     NAAIRENTEELEDNIAARKEARNVTEPINPAPTATIDEGPSEDELEAAEDVLRKRLEL
                     EKKYIEAELDLEIEVERKRADIWEDYWRDIKDADRDFWRDLEDAELDYNRSVEDLTTE
                     LAEKREDIDQESADKALEIQREYLRKLEDLRRKFQFDAQEAIRENDAIRFLQLRRQLE
                     YDRRTARIDRDRALEDQSITEDQKLEELKTYEERKRAELRLEYERELEDIRIKDARKR
                     QDAIIAREQELADLITYEERKRADLLTEYERELADFNVFQKEKEDGTAAALAELDRIV
                     RDGNQALADTESELYRTRIENFERFVNTYKRTAAGLQTLQDSNLVEDDTTTGGETTAD
                     QNRLRQLRIQAERIARSKNLLTDELMQTIANAKTITALQRIINDLIMQPIATTQNVEA
                     RATGGPVKPNRSYLVGEAGPELLTMGSFGGYVTPMMDANNFRVKLPNPTPVSNNNEFK
                     FVNSIQMLDPNTLSPGQIAIIQQVITQFMM"
BASE COUNT          383 a          356 c          315 g          561 t
ORIGIN      
        1 ccatcataaa ttgtgtgatc acctgttgaa taatagctat ttgaccgggt gataacgtgt
       61 ttggatcaag catttgaatg ctgttgacaa atttgaattc gttgttattg ctaacgggtg
      121 ttggatttgg tagcttgact ctgaaattgt tagcgtccat catcggtgta acatagccac
      181 caaagctacc cattgttaac aattctggcc ctgcttcacc aacaagataa ctacgatttg
      241 gtttaactgg cccccctgta gcacgcgctt caacattttg cgttgtggct attggttgca
      301 tgatcaagtc attgatgata cgttgtaatg ctgtgattgt tttagcattg gcaatcgttt
      361 gcattagttc atccgttagt agatttttgg aacgtgcaat acgctctgct tgtatacgca
      421 attgacgtaa acggttttgg tctgccgtag tttcaccacc agttgttgta tcatcttcaa
      481 ctaaattact gtcttgcaat gtttgcaaac cagccgccgt gcgtttgtaa gtatttacaa
      541 agcgttcaaa gttttcaatg cgtgtacgat ataattcgct ttcagtatcg gcgagtgcct
      601 gattaccgtc acgcacgata cgatctagtt cagcaagtgc tgcggctgtg ccatcttctt
      661 tttccttttg aaaaacatta aaatcggcaa gctcccgttc atactcggtt aacaagtcag
      721 cccgtttacg ttcttcataa gttatcaagt ctgccagttc ttgctctctg gcaataattg
      781 catcttgtcg tttacgtgca tctttaatcc gaatatcctc aagttcccgt tcatattcta
      841 aacgtagttc agcccgttta cgttcttcat atgtttttag ttcctcaagt ttttgatctt
      901 ctgttatact ttgatcttct aaagcgcgat cacggtcaat gcgtgctgta cgccgatcat
      961 attctaattg ccgacgtagt tgtaagaagc gaattgcatc attttcacga atcgcttctt
     1021 gcgcatcaaa ctgaaactta cgacgtaagt cctctaattt acgtaggtat tcacgttgta
     1081 tttctagcgc cttatcagca ctttcttgat caatatcctc gcgtttttca gcaagttcgg
     1141 ttgttaaatc ttcaacacta cggttataat caagttctgc atcttctaga tcgcgccaaa
     1201 aatcgcggtc agcatcttta atgtcgcgcc agtaatcttc ccaaatatcg gcacgtttgc
     1261 gctcaacttc aatttcaagg tctaattcag cttcgatgta tttcttttca agttcaagcc
     1321 gtttacgtaa aacatcttct gcggcttcga gttcatcttc acttggccct tcatctatag
     1381 tggcggttgg ggcagggttg atcggctcag tgacattacg ggcttcttta cgtgctgcaa
     1441 tattatcttc taattcttct gtattttctc tgattgccgc attttgtgcg ttaattgagt
     1501 cggcagtttc aaagaagtca tcaacatagt agccaactgt ggtacgtaat tggtcttgta
     1561 attgttgtcc ataatcttca aagattacgc ccccacttaa aggatcgact gtgaa
//
LOCUS       DAOPLS010000187         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_90958_length_1611_cov_1.837404, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000187 DAOPLS010000000
VERSION     DAOPLS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90958_length_1611_cov_1.837404"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKV52_03920"
     CDS             complement(<1..715)
                     /locus_tag="PKV52_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HPD99389.1"
                     /translation="MNDINIPRQVIVLANSIRKAGGRLYIVGGAVRDWQMGIPPIDLD
                     LLIVGLDITTGLAIMGQPEQVIGNAPVYMWGKTEVAFARRETSVGDGKHGFTFVADAT
                     VTLLEDLRRRDFTINSMAIDVLTGTFIDPFGGRHDIEKGLLHPTSRAFVESPERVLRG
                     AAFAARFDFDVTATFNTYARQMLVDFDTIPAEQIWRHWEKMCVHAIKPARWLEVIIDA
                     GWVYKFPVLAQMVGVEQDMW"
     gene            complement(756..1433)
                     /locus_tag="PKV52_03925"
     CDS             complement(756..1433)
                     /locus_tag="PKV52_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99390.1"
                     /translation="MMYTGVKDYRSVRRSLDRLVKLELIIISGGLVTVKTVPQLLVSK
                     EELCLLTRDPGTVDIADLHIDLIAERLLGQATKILNVLHQENTALDVKTLHLLTDIPK
                     RSIHRYLTKLDGLGLILADDRHYTLATDWLYTLYELADDSFNTNYLKIQAEYQRWLTI
                     KNNQKIYGTLKDALGHAAALTLIKRYEDENYVDDIIVTAIHNAGVPKPQFVLDWLSTK
                     VRLQNDL"
BASE COUNT          464 a          372 c          287 g          488 t
ORIGIN      
        1 accacatatc ttgttctaca cccaccatct gcgccaacac cgggaattta tacacccaac
       61 ctgcatcaat aataacttct aaccaacgcg ccggtttgat cgcatgtacg cacatctttt
      121 cccaatgccg ccagatttgt tcggcgggga tagtatcaaa atcaaccagc atttgccgcg
      181 cataagtgtt gaatgttgct gttacatcaa aatcaaaccg cgccgcaaaa gcagcaccac
      241 gcagcacccg ttctggtgat tccacaaatg cacggctagt ggggtgcaag aggccttttt
      301 caatatcgtg ccgcccacca aacggatcaa taaaagtgcc ggtcaataca tcaattgcca
      361 tactgttgat agtgaagtcg cggcggcgca aatcttcgag taatgtaaca gttgcatcgg
      421 ccacaaaagt aaagccatgt ttaccatcac caacgctagt ttcgcgccgg gcaaatgcca
      481 cttctgtttt accccacata tatacagggg cattcccaat aacctgttca ggttgcccca
      541 tgattgccaa tccagtagta atgtccaacc ctacaatcaa caaatctagg tcaattggtg
      601 gaatacccat ctgccaatca cgtacagcac caccaacgat atacaaacgc ccaccggcct
      661 tacggatact gtttgccagt acaataactt gtcttggaat gttaatatcg ttcattcacc
      721 aacctccaaa atttacttaa caatattata aaattttata aatcattttg caacctgact
      781 ttggtactaa gccaatctaa aacaaattgt ggctttggta caccagcatt atgtatagct
      841 gttacaataa tatcgtcaac ataattttca tcctcataac gtttaattag tgttaaagcg
      901 gcggcgtgcc ccagtgcatc ttttagtgtg ccgtaaattt tttggttgtt tttgatagtc
      961 agccaacgct ggtattctgc ctgtattttt aggtagttag tattaaaact atcgtcagcc
     1021 agttcataaa gtgtgtacaa ccaatctgtt gccaaagtgt aatgtcggtc atctgccaga
     1081 atcagcccta acccgtctaa tttcgtcagg tagcgatgta ttgaacgttt tggtatatcc
     1141 gtaagcaaat gtagtgtttt aacgtctaag gccgtatttt cttggtgcaa tacatttaat
     1201 atttttgttg cttggcccaa taaacgttcg gcaattaggt caatgtgtaa atcagcaatg
     1261 tcaactgtgc caggatcacg ggtaagtaga cacaattctt ctttacttac cagtaattgt
     1321 ggcacagttt ttaccgtaac taagccacca cttattataa taagctccaa cttaaccaat
     1381 ctgtctaacg atcttctaac tgaacggtaa tcttttacac ctgtatacat cattattcca
     1441 cgtttgctat atttaatttt gtaagtatca aactgaatac accttgcaat taaatattgg
     1501 tatacaactt tatctgttat atagcgccag ttatgcgtgg caaagtataa cgtttgtagt
     1561 gcttgtacag ttttactttt ggcaaggtaa cgatatgctt tatgtattgc t
//
LOCUS       DAOPLS010000188         1606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_91341_length_1606_cov_2.903288, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000188 DAOPLS010000000
VERSION     DAOPLS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91341_length_1606_cov_2.903288"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..603)
                     /locus_tag="PKV52_03930"
     CDS             complement(7..603)
                     /locus_tag="PKV52_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPD99391.1"
                     /translation="MLFRNSYKESAYYKTIEVTDRDARLLCAVKQHPFGLMVIYAASF
                     IAFVFALSLISIVLPKTMASTNSIYSAWTVLAFICGFVLVVVLIAITYVYNQSGLTVT
                     NKNVIQILQKSIYERKVSHISLANIEDVTSEQRGVFSTMFNFGTLKIETAGEQANFIF
                     PLCPHPNRVARIILDAKDDFIQATGQAGSYRNNININT"
     gene            complement(618..>1606)
                     /gene="ruvB"
                     /locus_tag="PKV52_03935"
     CDS             complement(618..>1606)
                     /gene="ruvB"
                     /locus_tag="PKV52_03935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPD99392.1"
                     /translation="EEQLELTLRPKDFASYIGQERLKQNLQLAITAAKKRNEPIDHVL
                     LFGPPGLGKTTMASVIANEMGAQIRITSGPAIERAGDLASLLTNLQDGDILFIDEIHR
                     LNRTVEEVLYSAMEDFKLDIMLGKGPSARSLRLDLPKFSIIGATTRTGALAAPLRDRF
                     GHVHRLDFYTPEEIARIIQRSSSILDTQVTKDAAAHIARRSRLTPRIANRLLKRVRDY
                     ADVNGDGIVDLQNAEQGLLLLEIDELGLDQSDRMLLATIIDNHQGGPVGVDTLAALLA
                     DERTTIEDFYEPYLLQIGMLERTPRGRKVTQKAYKHLNKVPPKSENSQTSFV"
BASE COUNT          440 a          327 c          322 g          517 t
ORIGIN      
        1 ataccactac gtatttatat tgatattatt tctataggaa cctgcttggc cagtggcttg
       61 tataaaatcg tcttttgcat caagtataat tctcgcaacc ctattaggat gggggcataa
      121 aggaaaaata aaattagctt gttctccggc tgtctcaatt ttgagtgtgc caaaattaaa
      181 catggtagaa aatactccgc gctgttcgct cgtcacatct tctatattgg caagagagat
      241 atgtgaaact ttacgttcgt atatactttt ttgaagtatt tgtataacgt tcttgtttgt
      301 cacggtaagg ccagactggt tgtacacata tgttatagcg ataagtacga cgactagtac
      361 aaaaccacat ataaacgcaa gtacagtcca agcagagtat atgctgttag tactagccat
      421 agtcttagga aggactatgc ttattaagct aagagcaaat acaaatgcaa taaacgatgc
      481 tgcgtagatg accatcaaac caaatggatg ttgttttacc gcacacaata aacgagcatc
      541 tctgtcggtg acttcgatgg ttttatagta agccgattct ttatagctat ttctaaatag
      601 catggttgta ttatagtcta tacgaacgaa gtttggctat tttctgattt tggtggcact
      661 ttattaagat gtttataagc tttttgtgta acttttctgc cgcgaggcgt tcgttctagc
      721 atacctatct gcagtaagta gggttcgtaa aagtcttcta tagtcgtgcg ctcatctgca
      781 agtaaagctg ccaatgtgtc tacgccaaca gggccccctt gatgattgtc tataatagtt
      841 gcaagcaaca ttctatctga ctgatcaagc ccgagttcat ctatttctaa taatagtaag
      901 ccttgttctg cattctgcaa atctacaata ccatcaccat tcacatcagc gtaatctcgt
      961 actcgcttga gcaatctgtt agcaatacgt ggcgttaagc gtgatctgcg tgctatgtga
     1021 gcagcagcgt cttttgttac ttgtgtgtct aaaatactcg aagatcgttg aataatacgc
     1081 gcaatttctt ctggtgtata aaaatcaagc ctatgtacgt ggccaaaccg gtcccttagc
     1141 ggtgcagcca atgctcctgt tcttgtagtt gcgccaatta tactaaactt aggtaaatca
     1201 agccggaggc tacgggctga tggtcccttt ccgagcatga tatctagctt aaaatcttcc
     1261 atggcgctgt agagcacttc ttctactgtt ctattgagcc gatgtatttc gtcaatgaac
     1321 aatatatcac cgtcttgtaa attagtaagt agacttgcca aatcaccagc ccgctcaata
     1381 gccggaccac tagtaatacg gatttgcgca cccatttcgt tagcaatgac acttgccatt
     1441 gttgttttac ctagcccggg tgggccaaac agtagtacat gatcaattgg ctcattacgt
     1501 tttttggctg ctgtaattgc taattgcaaa ttttgcttta aacgttcttg accaatgtaa
     1561 ctagcaaagt ctttggggcg taatgttagt tctagttgct cttcta
//
LOCUS       DAOPLS010000189         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_92023_length_1598_cov_2.354504, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000189 DAOPLS010000000
VERSION     DAOPLS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92023_length_1598_cov_2.354504"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKV52_03940"
     CDS             <1..353
                     /locus_tag="PKV52_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99393.1"
                     /translation="YKSEKYAYGQVDAACSVRIYLTYLTNSSDEALALAKKVTDKTRQ
                     LYVIEYEQQPNFTEISGSVEMDWSKNNFTYSNFSCTSWTSYNESSNVDGSHNTSVGIS
                     CGKHVKYYRIYPEV"
     gene            369..>1598
                     /locus_tag="PKV52_03945"
     CDS             369..>1598
                     /locus_tag="PKV52_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99394.1"
                     /translation="MYVYLVKNKARLFVLLLVAVGIAAILFTGYAMAIASTPYWNTEQ
                     GVTVSAGTSYNQSNLPANLLSEMPCSNDSYSNKTGTQQTICTVHTSGYKLTHDAKMVT
                     LGGTVLNPVINKLNQSSKLIPLDGSSSMLEIQGKQSSARLRIIKNAFSKAITDYQKTP
                     TTVYITSTDQYNLIDDANNTYLFSDYYLNTSADNTWVVSQNGNGIYKYNLVTNTIQYL
                     RTGYYSKNGYSPELMLAISESGRYVAVYTGRENKLEIIDTNTCTNSTAKRGQDAGCSS
                     RNITSDITNSISNLYLQNQFRYLQFENENTISFYAYKDWVNNQHFTLQKAYATSEGSA
                     QKRALDYLAMGDSYASGEGVYNYKDGTDIYGTNMCHLAMLSYPYIIANKGAAGDTESV
                     ACSGAKVKDILSFNKLAY"
BASE COUNT          559 a          300 c          305 g          434 t
ORIGIN      
        1 gctataagag tgagaaatat gcatatggtc aagttgacgc agcgtgcagt gtacgtatat
       61 acctaactta tttaacaaac agtagcgatg aggccttagc tttagccaaa aaagtcacag
      121 ataaaacaag acaactctat gtaattgaat acgagcaaca acctaacttt acagaaattt
      181 caggttcagt agagatggat tggtcaaaaa ataattttac atacagcaat tttagttgta
      241 ccagctggac cagctataat gaatcgtcaa atgtagacgg atcacataat actagtgtag
      301 gcatcagctg tggtaaacac gtaaaatact atagaattta ccctgaggta tagtttattc
      361 tatagacaat gtatgtgtat ttagtaaaaa ataaagcgcg actatttgtt ttattgctgg
      421 ttgctgtagg aattgctgct attctattta ctggctacgc catggcaatt gctagcacac
      481 cttactggaa tacggaacaa ggtgttacgg tatctgcagg cacgtcctac aaccaaagta
      541 acttaccggc aaatctatta agtgaaatgc cttgtagcaa cgatagctat agcaacaaaa
      601 caggcaccca acaaacaatt tgtacagttc atactagcgg gtacaaacta actcacgatg
      661 ccaaaatggt aactttaggt ggtactgtgc taaatccggt gattaataag cttaaccaat
      721 ctagcaaact tataccacta gatggcagta gtagtatgct agaaatacaa ggtaaacaat
      781 cttctgctcg gcttagaatt attaaaaatg cttttagcaa agcaatcaca gactaccaaa
      841 agacacctac gactgtgtac attactagca ccgaccagta taaccttata gatgatgcaa
      901 ataataccta cttgttttct gactactatt taaatacgtc tgcagacaac acttgggtag
      961 tcagccaaaa tggtaacggt atatataaat ataatcttgt cacaaatact attcagtact
     1021 tgcgtactgg ctactacagc aaaaatgggt attcgccaga actaatgctc gcaatttctg
     1081 aaagtgggcg gtacgtagcc gtatacacag gcagagaaaa taaactagaa attatagaca
     1141 caaatacttg cacaaacagt actgctaaac gagggcaaga tgccggctgt agctcgcgta
     1201 atatcacatc tgatattaca aacagcatat ctaatttata tctgcagaac caattccggt
     1261 acctgcaatt tgaaaacgaa aatacaatca gcttttatgc atataaagat tgggtaaata
     1321 atcaacattt taccctgcaa aaagcatatg ctactagcga aggcagtgca cagaagcgtg
     1381 ctttagatta tttagcgatg ggtgattcgt acgcttctgg cgaaggtgta tacaattata
     1441 aagacggtac agatatatac ggaaccaata tgtgccacct tgcaatgcta tcgtaccctt
     1501 atattattgc taataaaggc gccgcagggg acacagaatc cgtggcatgt agcggtgcta
     1561 aagttaaaga tatattgagt tttaataaat tagcttat
//
LOCUS       DAOPLS010000190         1571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_94231_length_1571_cov_3.596966, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000190 DAOPLS010000000
VERSION     DAOPLS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94231_length_1571_cov_3.596966"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..637)
                     /locus_tag="PKV52_03950"
     CDS             complement(143..637)
                     /locus_tag="PKV52_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99395.1"
                     /translation="MDPALIIIVIATAVFGIAPIVLRSSAVLIFLMLAAGELLSRLTA
                     QDATQFVQSLPSFSSLPTYSIVQIILLTIAPLIILFSYKNSSKPSQLFFHLLPAVASV
                     LVCVMLVTARLPYEVKDAVEQSNIYGTIEPFFSVAVAGGLLSSAFYLISTKPKHSKHK
                     KQLG"
     gene            complement(654..1010)
                     /locus_tag="PKV52_03955"
     CDS             complement(654..1010)
                     /locus_tag="PKV52_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPD99396.1"
                     /translation="MTYVNATIGSEQFSLELADTDAAREKGLSERDSIGKNNGMLFVF
                     DTAGDWRMWMVQMRFPIDIVWLNSDKEIVYIKHSATPAEYPEIYKAPQPSAYVIEVSA
                     GTLNRLGVQEGDSISF"
     gene            complement(1105..>1571)
                     /locus_tag="PKV52_03960"
     CDS             complement(1105..>1571)
                     /locus_tag="PKV52_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HPD99397.1"
                     /translation="HGNGVVLAETNKAAKAVMQAFYVEGVNFLVQEFIKESAGTDIRA
                     FVVGSRVVASIKRQSLDDDFRSNTHQGGVGKAIKLTDEEAKTAVKAAKAMNLRICGVD
                     MIRSDRGPLVLEVNSSASLKTPELITGRDIATKVIEYIERNAKQVKKDKVGA"
BASE COUNT          444 a          342 c          287 g          498 t
ORIGIN      
        1 tgcttaaaag gcaggtgctc taaccagctg agctatggtc ccgtattatc ttaccaagcg
       61 cttgttagat taatgcggta ctactatgct aaagaagcac gctttatttt ctcttaaatt
      121 gtcttttttg tcaatctgtt aattaaccca gctgtttttt atgtttagag tgttttggct
      181 ttgtagatat taagtaaaaa gcactggaaa gtaatccacc agctactgct acgctaaaga
      241 atggttcaat tgtaccgtat atattagatt gttctactgc atcttttact tcatacggaa
      301 gccttgccgt gacgagcatt acacaaacca gtacactagc tacagctggc aatagatgaa
      361 aaaatagttg cgaaggttta ctactatttt tgtacgaaaa taatattata agcggggcta
      421 tcgtcaacaa tattatttgc acaatactat acgttggcaa actgctaaac gacggtaaag
      481 actgtacaaa ttgagttgca tcttgtgcag tcaggcgtga taacaactcc cccgcagcca
      541 acatcaaaaa tatgagtacg gctgaagatc ttagcacgat gggggcaatc ccaaacactg
      601 ctgttgctat aacgataata attagtgctg gatccatact aaatagtgta cccctaaaag
      661 cttatgctgt cgccttcttg cacaccgagc cgattgagtg tacctgcgct tacttctata
      721 acgtaggcac ttggctgggg tgctttgtat atttcggggt attcggcggg tgtcgcgcta
      781 tgtttgatat acactatttc tttatcggaa ttgagccaaa caatatctat aggaaagcgc
      841 atttgcacca tccacatacg ccaatcacct gcagtatcaa aaacaaacag cataccgtta
      901 tttttcccta tactatcgcg ctctgataaa cctttttcgc gtgccgcgtc agtatcagcg
      961 agttctagac taaattgctc actaccaata gtagcgttca cataggtcac agtatttagt
     1021 ctatgtataa aaaggtatga tgctaaacct ataaaagcca gcagtacagt aactacaagt
     1081 gcgctataaa caaaactttt ttgtttaggc cccgacttta tcttttttaa cttgttttgc
     1141 atttctctct atgtattcta taacttttgt ggcgatatcc ctacctgtaa ttaattctgg
     1201 tgtttttaaa cttgctgaag aatttacttc taaaacaagt ggccctctgt cgctacgtat
     1261 catatcaaca ccgcagatac gcaaattcat agctttagcg gcctttacag cagtcttagc
     1321 ttcttcgtcg gttagtttta tggctttgcc aacgccaccc tggtgtgtgt tacttctaaa
     1381 atcatcatct aaactttggc gctttatact ggcaaccact cggctaccta ctacaaaagc
     1441 acgtatgtca gtgccggctg attctttaat aaattcttgt accaaaaagt taacaccttc
     1501 tacataaaaa gcttgcatga cagcttttgc agctttgtta gtttcggcaa gcactacccc
     1561 attaccatgt g
//
LOCUS       DAOPLS010000191         1563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_94974_length_1563_cov_2.781167, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000191 DAOPLS010000000
VERSION     DAOPLS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94974_length_1563_cov_2.781167"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKV52_03965"
     CDS             <1..396
                     /locus_tag="PKV52_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99398.1"
                     /translation="PDSIKLDENPMPIFRPKGFTTHPIGDGGKKQIMPNNDAINIAEV
                     SKKSENLLHNVKAVDAESKLNNVAIKQALKAQVDDIKNKKGEHDLAKDYLARAGNKSG
                     GSLQISEQAAALVNVQQSKPKYSEITKKN"
     gene            complement(435..>1563)
                     /locus_tag="PKV52_03970"
     CDS             complement(435..>1563)
                     /locus_tag="PKV52_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD99399.1"
                     /translation="SFGDLLVFMTYMGYLLNPVQELVSQVTSRNKKLIDVHRVYEVLS
                     DHEGIENLRKDSLLPPRLSGIVRFENVSYSYKDNLVLNNVNFTVNAGEKIGIIGPSGG
                     GKSTILKLVPLFIEPDGGRVLIDEYDTQTVSLHDLRKRIAWVSQTPQLFDGTILDNLY
                     DADIDRQITSDEVVDALDVANVTEFAVKLPLGLDTPTGENGGSLSGGQRQRVAIARSL
                     LRGSPIICLDEPTAALDAKSENYIKDSLSQMIQGKTVMMVTHRKALLALMDTIYVLEN
                     STLTNVNELGGLDAYLAKLEGLEIENAQIDNEHIEPIAPIGGGSGGVPEVTDVDKAIN
                     DALATVDDTPPIATNNDETLNDTVPTETTDNDGTLRIDHHQ"
BASE COUNT          442 a          325 c          319 g          477 t
ORIGIN      
        1 ccggattcta ttaaactaga tgaaaacccg atgcctattt tcaggccgaa aggcttcact
       61 acccatccaa taggtgacgg cggtaaaaaa caaattatgc caaataacga tgctataaat
      121 attgctgaag tttcaaaaaa aagtgagaat ttattgcata acgtaaaggc tgttgacgct
      181 gaatctaaac taaacaatgt tgcaattaaa caagcgctta aggcacaagt agacgatata
      241 aagaataaaa aaggcgagca tgatttggct aaggattacc tagcaagggc tggaaataag
      301 tccggtggct cattacaaat aagtgaacag gctgcggctt tagtgaatgt gcaacaatct
      361 aaaccaaaat atagtgaaat taccaaaaag aattaagctt gatagtgtag gtatagtact
      421 ttataaatgt agttttattg gtgatgatct atacgtagcg taccatcatt gtctgttgtt
      481 tctgttggca cggtgtcatt tagtgtttcg tcgttgttgg ttgctatagg cggagtatca
      541 tcaactgtgg ccaaggcatc gttaatcgct ttatctacat ctgtaacttc tggtacacct
      601 ccagagccac cgccaatcgg tgcaatcggt tctatgtgtt cattgtctat ttgtgcattt
      661 tctatttcta gaccttctag tttagccaag tatgcatcta gcccgccaag ttcattaaca
      721 tttgtaagcg tgctgttttc tagtacataa atcgtatcca taagagcgag tagtgcttta
      781 cggtgcgtca ccatcataac ggtcttgccc tgaatcattt ggctgagtga atctttaata
      841 taattttcgc tttttgcatc aagtgcagct gttggctcgt caagacaaat aatgggcgat
      901 ccgcgtagta gtgaacgcgc aatcgctacg cgctgacgtt gaccaccact taggctaccg
      961 ccattttcac ccgtaggtgt atctagccca agcggtagct taacggcaaa ctctgttacg
     1021 ttggcaacat cgagcgcatc tacaacttcg tcgctggtta tttgtctgtc tatatcggcg
     1081 tcgtataaat tatctagaat cgtaccgtca aatagttgtg gtgtttggct cacccaggcg
     1141 atccgctttc gtaaatcgtg tagcgatact gtttgcgtgt cgtactcatc tatgagcaca
     1201 cgcccaccat ctggttctat aaataacggt acaagcttta aaattgtact tttaccacca
     1261 ccacttggcc caataatacc tattttttca cctgcattaa cggtgaagtt gacattattt
     1321 agcactaagt tgtctttgta gctatagcta acgttttcaa accgtacgat gccgcttagg
     1381 cgcggtggta gcaagctatc tttacgtaag ttttctatgc cttcatgatc acttaaaact
     1441 tcgtatactc ggtgcacatc tataagtttt ttattgcgtg aagtaacctg gctaaccagt
     1501 tcttgcacag ggttaagtaa gtagcccata tacgtcataa agactagtaa atcaccaaaa
     1561 cta
//
LOCUS       DAOPLS010000192         1544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_96649_length_1544_cov_3.566152, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000192 DAOPLS010000000
VERSION     DAOPLS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96649_length_1544_cov_3.566152"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..672)
                     /locus_tag="PKV52_03975"
     CDS             complement(277..672)
                     /locus_tag="PKV52_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD99400.1"
                     /translation="MDLQHGDATGKKILLVEDDEGLASVYQTRLEAENFTVRRVPNGE
                     DALAAAIEFKPDLILLDAMMPKVSGFDVLDILRNTPETTNIRIIMLTALSQEADQQRA
                     EELGVDEYLVKSQVVIADVVERIKHHLGL"
     gene            complement(731..>1544)
                     /locus_tag="PKV52_03980"
     CDS             complement(731..>1544)
                     /locus_tag="PKV52_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPD99401.1"
                     /translation="PLQGYPAEAAGLKAKDIIIEIDGQDATGISTAEAVKRIRGEEGT
                     SVKLVVVRNGNERLEFTIVREKIDVPSLETSYTDDGTIGIISISQFSQDTLGLMDKAV
                     AEFTQKGVKGIIVDVRNNPGGFLNSSVDIAGKWLTPGQTIVEERRDNKTVEKFSAKTK
                     GALNGIPTVVLVNEGSASASEILAGALKDNGAAKLVGVTTFGKGSVQETVRYDKGGLL
                     KVTVARWFTPNGQNISESGIEPDVKVEITEQDVTDKKDPQKDKAIAELKAQF"
BASE COUNT          369 a          342 c          288 g          545 t
ORIGIN      
        1 gacagtagcg ctgctctttc accgaaattt cttggtattt ttaacttgta tggtaattga
       61 agaataccaa tagtgtctgg gtagtcttgt atagtttctg gagctttatc aatttgttgt
      121 agcattttta tatttaaata atatgtatgt atagtagtat attttttaca atttgtcaat
      181 atatggctgt tctataccat cacacaaggt acattgagca caaatagagc aaccataagt
      241 attgctaccc taagaacttg ctattagtat taatttttac aggccgaggt ggtgtttaat
      301 gcgctctacg acgtctgcaa tgacaacttg gctttttacc aagtattcgt ctacaccaag
      361 ttcttctgca cgttgttggt ctgcctcttg gcttaatgcc gtaagcataa taatacgaat
      421 attggttgtt tctggagtgt tacgcaaaat gtctagtaca tcaaaaccac ttacttttgg
      481 catcatggcg tctagcaaaa ttaaatcagg cttaaattct atagcagcag ctagcgcgtc
      541 ttcgccattt ggtacgcgcc gtaccgtaaa gttttctgct tctaaccgag tttgatagac
      601 acttgctaaa ccttcatcgt cttctacgag taatattttt ttaccagtag cgtcgccgtg
      661 ttgtaaatcc atatgcgtat gtttccttat tatttatcta taccttatat tataccactc
      721 acgcgttaag ttaaaactga gctttcagct ctgcaatcgc tttgtctttt tgaggatctt
      781 tcttatctgt tacgtcttgt tcggtaattt ctactttaac gtctggttca ataccgcttt
      841 cgctgatgtt ttgaccattt ggcgtaaacc accgtgccac agttactttt agcaagccgc
      901 ctttatcgta acgaacagtt tcttgcacgc tacccttacc gaatgtcgtg acaccaacta
      961 atttggcagc accgttgtct ttaagcgccc ctgcaagaat ttcgctggca ctggcactac
     1021 cttcgtttac taatacgacg gttggaatgc catttagcgc accctttgtt ttagcgctaa
     1081 acttttctac cgttttgtta tcgcggcgtt cttctacaat tgtttgcccg ggggtgagcc
     1141 acttacctgc aatgtctaca gaactgttta agaatccacc tgggttatta cgcacatcta
     1201 caataatgcc ctttacccct ttttgggtaa attctgccac agctttgtcc ataagcccca
     1261 aggtgtcttg tgagaactga ctaatagata taattccaat agttccatca tctgtgtagc
     1321 tggtttctag gcttggtaca tcgatttttt cgcgtacaat ggtaaattct aagcgttcgt
     1381 taccgttgcg tacaaccaca agttttacac ttgtgccttc ttcgccacga attcgtttta
     1441 cagcctcggc tgtgcttatt ccagttgcat cttggccgtc aatttctata attatatctt
     1501 tagcttttag cccggctgct tcggctgggt agccttgcaa agga
//
LOCUS       DAOPLS010000193         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_97124_length_1539_cov_3.553908, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000193 DAOPLS010000000
VERSION     DAOPLS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97124_length_1539_cov_3.553908"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1539)
                     /locus_tag="PKV52_03985"
     CDS             complement(<1..>1539)
                     /locus_tag="PKV52_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HPD99402.1"
                     /translation="DDIVLRAIVKYPNITATHLYRLAKRLIEGTRADRHNIQGYNELA
                     YPKKPLWASRDTLALTRVNQVRVGLKHAGVVLRHPRLQDLPYPPDRFIVPEDVPVLID
                     YNINDCLITRLMYYELLPIIRMREDISKMFEVDVYSANDTEIAKEILAKEYMAKTGIA
                     WTTLKEMRTERGVIQGSDCIGKNIRFDTPKLQQLLAYISKQRLYPSNNYKYKLEVEFD
                     DVHYKIGVGGLHSDDKPRILVSTADVTIRDADVTSFYPFILINNGFYPAHLGEIYPVI
                     YQSIVDQRVIAKKLAKKGDVAAAIRQAGLKIAINSGFGLMGNRYSWLYDPQALVSITI
                     SGQLYLLDLIEKLYLAGIHTLSANTDGIVCEVPVSKETTYQSVCAEWQARTNFNLEFA
                     NYAQLIQRDVNNYLSIKDSGEVKTKGIFQNDAGFRWDFGNGMGFYDTNLYNHAINGLI
                     PTEDELASNRIIPGFSRGFSKPVVSIALYQYFVHGVDPAITIHNHRDVHDFIISQRAD
                     ANKF"
BASE COUNT          443 a          332 c          279 g          485 t
ORIGIN      
        1 tcaaacttgt tagcatcggc acgttggcta atgataaagt cgtgtacatc acgatggttg
       61 tgaattgtta tggctggatc aaccccatgt acaaaatatt gatataatgc aatgctcacc
      121 actggttttg aaaagccgcg tgaaaagccg ggtatgatac ggttgcttgc caattcatct
      181 tcggttggta ttaaaccgtt aatcgcgtga ttgtaaaggt tggtatcgta aaaacccata
      241 ccattaccaa aatcccaacg aaatcccgca tcattttgga aaatgccttt agttttcact
      301 tccccactgt ctttaattga taggtaatta ttcacatcac gttgtattaa ctgtgcatag
      361 ttagcaaatt ctaaattgaa atttgttctt gcctgccatt ctgcacaaac gctctgatat
      421 gttgtctctt ttgatacagg tacttcgcac acaataccat ctgtgtttgc tgataacgtg
      481 tgaataccag ccaaatacaa tttttcaatt aggtctagta agtataactg tccactaatt
      541 gtaatcgaaa ctaatgcttg cggatcgtac aaccaactat atcggttgcc catcaagccg
      601 aaaccgctgt taatggcaat tttcaaacct gcctgtctaa ttgcagctgc aacatcaccc
      661 tttttcgcca actttttagc aataacacgt tgatccacaa ttgattgata gataacaggg
      721 taaatttctc ccaagtgggc tggataaaac ccgttgttaa tcaatataaa tgggtagaaa
      781 cttgttacgt ccgcatcccg gattgtgaca tcagccgtac ttactaagat acgtggttta
      841 tcatcggaat gcaaaccacc aacaccaatt ttataatgta catcatcaaa ttcaacctct
      901 aatttgtatt tgtagttatt tgatgggtac aaccgttgtt tactaatata tgccaataat
      961 tgttgtaatt tcggtgtatc aaagcgtatg tttttgccga tacagtcact accttggatt
     1021 acaccccgct cagtacgcat ttcctttaat gttgtccatg cgatacctgt ttttgccatg
     1081 tattccttcg ccagaatttc tttagcaatt tccgtatcat ttgccgaata tacatcaact
     1141 tcaaacattt ttgatatatc ttcacgcatt ctgataatgg gcaacagttc ataatacatg
     1201 aggcgggtta tcaaacagtc attgatatta tagtcaatca aaacgggtac atcttcgggc
     1261 acaataaacc gatctggcgg atacggtaag tcctgtaggc gtggatgacg taatacaaca
     1321 cccgcgtgct tcaaaccaac acgcacttga ttaacccgcg ttaacgccaa tgtatcacga
     1381 gaagcccaca atggtttctt agggtatgcc agttcgttgt aaccctggat attatgtctg
     1441 tcagcacgtg taccctcgat taaccgcttt gctaaacggt acaagtgtgt ggctgtaata
     1501 ttgggatatt ttacaattgc acgtagtaca atatcatca
//
LOCUS       DAOPLS010000194         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_98060_length_1529_cov_3.875848, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000194 DAOPLS010000000
VERSION     DAOPLS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98060_length_1529_cov_3.875848"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKV52_03990"
     CDS             <1..697
                     /locus_tag="PKV52_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006294704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD99403.1"
                     /translation="QPLFTDFNVSMKAGQKVGLVGPSGGGKSTITKLLLRMMDVTSGE
                     ISIDGQNITDVAQDELRNVIAYVPQEPILFHRTLKENIAYGHPQATQQQIIAAAKAAH
                     ADEFIALLPKGYDTLVGERGVKLSGGQRQRVAIARAMLKNAPILLLDEATSALDSDNE
                     QLIQKALWSLMNNRTAIVIAHRLSTIQKMDRILVIDNGIIIEDGTHETLLQQNGLYAS
                     LWAHQSGGFVVDK"
     gene            736..1470
                     /locus_tag="PKV52_03995"
     CDS             736..1470
                     /locus_tag="PKV52_03995"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPD99404.1"
                     /translation="MKPIPINVIKRIAILVSIVAVVIFLMTRHIDIALLLETGGIVAI
                     GLTIFAETGLLIGFFLPGDTLLFAAGFFASQDKISLIGSIVAVSIGAIFGNMLGYEIG
                     RRSGKRFFKKEEAVLLNKETVNAANAFYIKHGGKTVILARFIPVVRTLAPIMAGIGKM
                     TYKRFFIYTVVGALIWGIVVTLVGYWAGLVIGQYFDIDKYLLPLIFLAVLGTFGISVF
                     HVLKDKKSRDLVIQKSKSYITNFFKN"
BASE COUNT          474 a          274 c          330 g          451 t
ORIGIN      
        1 acaaccgtta tttaccgatt ttaatgtatc tatgaaagcc ggccagaaag tagggttagt
       61 aggccctagt gggggtggca aatctactat tacaaaattg ttactgcgta tgatggatgt
      121 aacaagcgga gaaatttcaa tagatggcca gaatattaca gacgttgcac aagatgaatt
      181 acgtaacgta atagcctatg ttccgcaaga gccaattttg tttcatcgca cgctcaaaga
      241 aaatatagcg tatgggcacc cacaagcaac acaacaacag atcatagcgg cggccaaagc
      301 agcacatgct gatgagttta tagccttatt acccaaaggg tacgatacac tcgtaggaga
      361 aaggggtgtt aagctttctg gcggacaacg gcaacgagta gcgattgcac gtgccatgtt
      421 aaaaaatgct ccgatacttc tcctagacga agcaaccagt gctctagata gtgacaacga
      481 gcaactcatc caaaaagccc tttggagctt aatgaataac cgtacagcaa tcgtcattgc
      541 acaccggcta agtacaattc aaaaaatgga tcgtatttta gttatagaca acgggattat
      601 tatagaagac ggtactcatg aaacgctact tcagcaaaac ggtctctatg catcgctgtg
      661 ggcacaccaa tcaggtggct ttgttgtcga taaataagtg gagcgttttg gttcgttact
      721 atatacttac atagagtgaa accaatacca ataaacgtca ttaagcgcat tgctatttta
      781 gtgagtatcg ttgctgttgt tatttttttg atgacgcggc acatagacat agctctctta
      841 ctagaaactg gtggtatagt tgcaattgga cttactattt ttgcagaaac tggtttgctt
      901 attggtttct tcttaccagg tgataccctg ttatttgctg cgggtttttt tgcatcgcaa
      961 gataaaataa gtctaatagg cagtatagtt gctgtatcta taggtgcaat atttgggaac
     1021 atgctggggt atgaaatcgg tagaagaagc ggaaaacgat ttttcaaaaa agaagaagcc
     1081 gttctgttga ataaagaaac agtaaatgca gcaaacgcct tttacattaa gcatggtggt
     1141 aaaaccgtta tactcgcgcg atttatacca gttgtcagaa cgctcgcccc tatcatggct
     1201 ggtattggta aaatgaccta caaacgtttc tttatataca cggtagtcgg agcacttatt
     1261 tggggtattg tggtaacact cgttggctac tgggcgggtc tagtaattgg gcaatatttt
     1321 gacattgata aatatttgtt accgttaatc tttctcgcag tgctgggcac gtttggtatt
     1381 agtgtattcc acgtattaaa agataaaaaa tcacgtgatt tagttataca aaaaagtaaa
     1441 tcgtatatta caaatttttt caaaaactag ctatttgtat tgattatata taattctttc
     1501 taaagcctga gatttgtgta taaaggcca
//
LOCUS       DAOPLS010000195         1526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_98369_length_1526_cov_2.470428, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000195 DAOPLS010000000
VERSION     DAOPLS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98369_length_1526_cov_2.470428"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1526)
                     /locus_tag="PKV52_04000"
     CDS             complement(<1..>1526)
                     /locus_tag="PKV52_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99405.1"
                     /translation="PLRFGSDVNLYRGAANRLDIASGDSLNVISGQIMTDAGNLSVLT
                     TASGATNIRARGLLLGSSYDSTPLQNEIRSTNNQNLILNSRGTGNLYLQTADTTKLTV
                     QNNGNVGIGTTTPNTALDVNGVTSRSQNNVRTYKNVYTYNISASNQTGTIVLTAPKGF
                     TATMLRFTIKGYDYSGYGAWEAIVSGYAYTTPSWVNASAEIRGSAPFTTIRLASNGTN
                     PVIMLGNTSTVWQYPKVVIEEIIAGHSSIDGWGDAWSGALVTDETTPINYTLINTPSQ
                     SGPFIQNGNSFGTTATLGTNDSQALVLETANTARLTITNAGAATFSGTLAVTSTSTFT
                     GLGTFNGGITVANNSNFSQTGSGTFSTGTGANTLNGATSITSTLSVTGNATFDTNTLF
                     VDAALNRVGIGTASPSRKLEVYGGHGDTQLLLHSIGDGGATNTADLMLWASEPGHTYT
                     GVGIANNLYNTTGFPRINTNRGGSMIRLLDNSMRFSTVNSSGTQLDGLVLDGSGNLTA
                     A"
BASE COUNT          377 a          325 c          357 g          467 t
ORIGIN      
        1 gctgccgtta ggttgccaga tccatctaag accaagccgt ctagttgggt gcccgatgag
       61 ttaacggttg aaaagcgcat tgaattatct aacaagcgaa tcattgagcc accgcggtta
      121 gtgtttatgc gtgggaagcc agtggtgttg tataggttgt tagctatacc gacgccagta
      181 tatgtgtgcc ctggttcgct cgcccacagc ataagatctg ctgtgttagt tgcaccacca
      241 tcaccgatag agtgaagcaa tagttgcgta tcaccatgac caccatatac ttctaacttg
      301 cggctaggcg aagccgtgcc tatgcctact ctgttgagag ctgcatctac aaatagtgtg
      361 tttgtgtcaa aggtagcgtt accagttact gatagagttg atgtgatgct ggtagcacca
      421 ttcaacgtat tagcaccggt gccagtgcta aaagttccgc taccggtttg gctaaagtta
      481 gagttattag ctacagttat accaccgttg aaggtaccaa ggcccgtgaa ggtgctggtg
      541 ctggttacag ctagtgtacc gctaaatgtt gcagcaccag catttgtaat agttagtctt
      601 gcggtatttg ctgtctctaa tacaagtgct tgtgaatcgt tagtgcccag agttgccgta
      661 gtaccaaagc tgttaccgtt ttggataaaa ggtccgcttt gggaaggcgt atttattaaa
      721 gtgtaattaa taggtgtagt ttcgtctgtt actaatgccc cactccatgc atcaccccag
      781 ccatctatac tgctatggcc tgctattatt tcttctataa ccaccttggg gtactgccaa
      841 actgtagaag tattgcctag catgataact ggatttgtac cgttactagc tagtctaatt
      901 gtagtgaatg gtgcactacc gcgtatttcg gccgaagcat ttacccagct tggagtagtg
      961 taagcatatc cgctaactat tgcctcccac gcaccatagc ccgaataatc atagccctta
     1021 atagtgaatc ttaacatagt agcagtaaag ccttttggcg cggtaagcac tatagtacca
     1081 gtttggtttg aagcagaaat gttatacgta taaacatttt tatatgttct tacgttgttt
     1141 tgagacctac tagtgacgcc gtttacatca agtgctgtgt tcggcgtagt cgtacctata
     1201 cctacattac cgttgttttg cacggttagt ttagttgtgt ctgcagtttg cagatataga
     1261 ttacctgtac cgcgtgagtt aagtattaga ttttggttat ttgttgaacg aatttcgttt
     1321 tgtagtggcg tactgtcgta tgatgatcct agtagcaagc ctctagccct tatattggta
     1381 gcaccactag cagtagttag taccgaaagg ttaccagcat ctgtcatgat ttgaccacta
     1441 ataacattta ggctatcccc gctggctatg tctaggcggt tagctgcacc acggtataag
     1501 ttaacgtctg agccaaaccg tagtgg
//
LOCUS       DAOPLS010000196         1523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_98652_length_1523_cov_4.024523, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000196 DAOPLS010000000
VERSION     DAOPLS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98652_length_1523_cov_4.024523"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..358)
                     /locus_tag="PKV52_04005"
     CDS             complement(2..358)
                     /locus_tag="PKV52_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99406.1"
                     /translation="MPEVYIVTKIGNGNTNLFKSILEKIGQNEAVPLLVRTLGETGLK
                     NADLFVNNIFITQENDPFTVPDEHPGPMPIIYIATGVAALQGEDLRSASLTIHGQPSD
                     TTPQCELELADKNSLS"
     gene            complement(358..1260)
                     /locus_tag="PKV52_04010"
     CDS             complement(358..1260)
                     /locus_tag="PKV52_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99407.1"
                     /translation="MTQIRTPELVNTKAETLFRQQVRGGIQVANQAHPYLATRGFYGS
                     IDTITQMNIPDLAKATAALLLAIPNLARMVASNGGDVSICSTMHTAMGTLMDSSYQTS
                     ITKNNVLSMCMDFNKQQNICTGEAIKNLEEQLLADLAGVWHEHSWENLLIAADINVER
                     ANRGEDLAGTDLWLQLPDAQKWVSVDIKARPETILRHAHKKGYEITDDYTFDAHGVTA
                     QDFELYIAASTEHRTAYLSFNNPPGSDGLAPIDFRGNHNAIVASSDIFATLQYYEDSG
                     LIKPYRQLETTDGTHYVPFDRRIN"
BASE COUNT          378 a          323 c          323 g          499 t
ORIGIN      
        1 tttaacttaa actgttttta tcggcaagct ctagctcgca ttggggtgta gtgtctgatg
       61 gttgcccatg aatagttaag ctagcagatc ttagatcttc gccttgtagt gccgctacac
      121 ctgtggctat atagattata ggcatcggtc cagggtgttc gtcaggtaca gtaaacgggt
      181 cattttcttg cgttataaaa atattattaa caaataaatc cgcgtttttg aggcccgtct
      241 ctccaagggt acgcacgaga agtggtacag cttcattttg acctattttc tctagtatac
      301 tcttaaataa gttagtgttt ccatttccta ttttggtaac aatatacact tctggcatta
      361 gtttattctc ctatcaaagg gtacgtaatg tgtgccgtct gttgtttcta attgtcgata
      421 tggctttatt aagccgctat cttcgtaata ttgcaaggtt gcaaatatgt cgctactggc
      481 gacgatggca ttgtggttcc ccctaaagtc aatgggcgct aaaccgtcac taccgggtgg
      541 gttattgaat gacaggtagg ctgtgcgatg ttcggttgag gcagcaatat aaagttcaaa
      601 atcttgagct gtaactccat gcgcatcgaa ggtataatcg tcagttattt cgtagccttt
      661 tttatgagca tgccgcaata ttgtttctgg ccgagcctta atatctacag acacccactt
      721 ttgtgcatca ggtagctgta accataagtc tgtaccagcc aaatcctcac ctctgtttgc
      781 tcgttctaca ttgatgtcgg cagctatgag taaattctcc catgaatgct catgccagac
      841 acccgcaaga tctgctagta gttgctcttc tagatttttg attgcctctc cagtacagat
      901 attctgttgt ttattaaagt ccatacacat actaagtaca ttattttttg tgatacttgt
      961 ttggtatgag ctatccatta acgtgcccat ggctgtgtgc attgttgagc aaatagacac
     1021 atcaccaccg ttactcgcaa ccatacgtgc caaattagga attgctagta atagagcggc
     1081 agtagctttc gctaagtcag gaatattcat ctgcgtaata gtgtctatag aaccataaaa
     1141 tccccttgta gctaagtacg ggtgcgcttg attcgcaacc tgtataccac ccctcacttg
     1201 ttgtctaaag agtgtttcag cttttgtatt tactagttct ggtgtacgta tttgtgtcat
     1261 ttttaatatt gttattaagt agtattaata tagcacatta ctaaaataaa gtcaattttt
     1321 tacagatgcg aaaagcccga taggctctgt ttttgctcct gcgcgttagt atgcgcgggc
     1381 taacagttgc tatcgggctt atatgatact acagagggtg gccaagaggg ccacgagtgt
     1441 attgcatctc gcagccctct tgccacaaac cttgccccta gactgcgtgc ctgcacgcag
     1501 ccacagtgat gagtgtcttc tgg
//
LOCUS       DAOPLS010000197         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_99472_length_1514_cov_4.718300, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000197 DAOPLS010000000
VERSION     DAOPLS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99472_length_1514_cov_4.718300"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PKV52_04015"
     CDS             <1..836
                     /locus_tag="PKV52_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99408.1"
                     /translation="CDNTPLIGQPCDPPVGFDASTAIITNQTGISGFTIAPGATANSL
                     LLTRVPSFVNAADIRIELSGITNPTNKGTYFGRIQTYSSDDATGVAVNEGGVTFAITP
                     PIDIATYVPPYLYLCVAVTLPGYLCSGAVGNFVDFGELSANQTRTGTSQFLVATNAGA
                     GFVVYVIGIPPTSGNNVIPAMTTRGPSQTGVSQFGINLRDNSNPDVGNDPVGAGSGTI
                     APDYNIPNQFKFFYGQALVSSNTTTADKKFTTSYIINVNRNQKPGVYNTTLLYIALAT
                     F"
     gene            901..>1514
                     /locus_tag="PKV52_04020"
     CDS             901..>1514
                     /locus_tag="PKV52_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99409.1"
                     /translation="MKHKRILSLAAVFVVAGSLLTQAVFAQESGNGFQISPVRTELTI
                     EPGGSEKTTVTAMNPTSGAIKANVVVNDFEAATDETGQPKVIFQSDQTAPGNSFKSLV
                     GDLGTVDIPAGGSVNIPVVVTVPEGASPGGYYGAVRLEPTVETDKNVNLSASVGTLYL
                     VTVPGDLNEALELVEFTAAKNGSTGRFFIGGGENIQIVTRLKNTG"
BASE COUNT          457 a          290 c          333 g          434 t
ORIGIN      
        1 tttgtgataa tacgccgtta atagggcaac catgtgatcc gccagtgggc tttgacgctt
       61 ctactgctat aatcacaaat caaactggaa taagtgggtt cactatagca ccaggtgcta
      121 ctgcaaatag tttattacta acaagagtgc cctcatttgt taatgctgct gatatacgca
      181 ttgagttaag tggtattaca aaccctacta ataaaggtac atattttggt aggattcaaa
      241 cgtatagtag tgatgacgct actggtgtag cggttaatga aggtggtgtc acgtttgcga
      301 tcaccccacc gatagatata gcaacgtacg ttccaccgta tctttattta tgcgtcgccg
      361 ttacactgcc gggctattta tgtagtggcg cagttggtaa ttttgttgat tttggtgaat
      421 tgtctgccaa ccagacacgc acaggtacct cacaattttt ggtagccact aatgctgggg
      481 ctggttttgt tgtatatgtt attggtatac cacccacatc tggtaataat gtaataccag
      541 ccatgaccac ccgaggtcct tcgcaaacag gggtgagtca gttcggtata aatttacgag
      601 ataattctaa cccagacgtt ggcaacgacc ctgttggtgc tggcagtggc actattgctc
      661 cggactataa tattcctaat cagtttaagt ttttttatgg ccaagccctc gtaagtagta
      721 acaccacaac tgctgataaa aaatttacga caagttatat cattaatgtt aatagaaatc
      781 agaagccagg tgtctacaat acaacacttt tatatattgc acttgcaaca ttctagtaaa
      841 ataacttatg ctttgatata gttaaaagta agtaattaaa aagtacatat gagggagaat
      901 atgaaacaca aaaggatttt aagtttggca gcagtttttg tcgtggctgg ttcattgcta
      961 acacaggctg tgtttgcaca agaaagcggt aatggctttc agatttcacc tgttcgcaca
     1021 gagcttacaa tagaaccagg tggttctgaa aaaacaaccg taacagcaat gaacccgaca
     1081 tcaggagcaa ttaaagcaaa tgtcgttgta aatgactttg aagcagccac agacgaaaca
     1141 ggccaaccaa aagttatctt ccagagcgac caaactgcac caggaaatag ttttaaatca
     1201 cttgtaggtg atttaggtac ggtagatatt ccagctggag gcagtgtgaa tataccagtt
     1261 gtcgttacgg taccagaagg tgccagccca ggtggttact acggcgctgt gcgtttagaa
     1321 cctacggtag aaactgataa gaacgttaat ttatcggcaa gtgttggtac gctttatcta
     1381 gttacagttc ctggtgattt aaacgaagcg cttgagcttg tagaatttac agctgcaaaa
     1441 aatggttcaa caggaagatt cttcattggt ggtggtgaaa acatacaaat tgtcacgcgc
     1501 cttaaaaaca ctgg
//
LOCUS       DAOPLS010000198         1512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_99680_length_1512_cov_4.057653, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000198 DAOPLS010000000
VERSION     DAOPLS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99680_length_1512_cov_4.057653"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..909
                     /locus_tag="PKV52_04025"
     CDS             31..909
                     /locus_tag="PKV52_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99410.1"
                     /translation="MTWQQVMVLQIIASSSMTLWYRKVSLVYSKHTFTLFLTIYLTVA
                     VAGSIISVVRFGGTLPVLPTGTVVWYLIGEGLFIPMSWYFSYRLLSIIGASSMAVAQS
                     FIFLTTATLGILLLDEPLTVGLFVGALLLLSGVYLALSVNGRHVKKNNVTLTKKLYLL
                     SMSALLLSIGLLFEKLAINSIGVWDYAMFGWAAQGAGAVILFSIFGRQELGLQRTARF
                     WRYALVAGLLTTLTGSLFIYALSTSMLSSVVLASTAKITLTSVLAFWLLKERTYLGKR
                     IAAVALAVCGLLAIFA"
     gene            952..1491
                     /locus_tag="PKV52_04030"
     CDS             952..1491
                     /locus_tag="PKV52_04030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPD99411.1"
                     /translation="MGHTIQQIRQNCTKARETMANARVQKFAVGAFNIDNQETLIAVA
                     KAAQKLQAPLMVEVSHGEVEAMGLMNVRDMVDNYKQEYGIEMYINLDHSPSVETAKAG
                     IDAGFEFIHIDVSQANHNATDNEIIIATREVVRYATFTGAIVESEPHYFGGSSNVHKE
                     AFDYADIKKTFSTPRGAEK"
BASE COUNT          425 a          286 c          340 g          461 t
ORIGIN      
        1 tgttgtctgc aaccggaaag caatagttct atgacctggc aacaggtaat ggtgctacaa
       61 ataattgcta gttcaagcat gacattatgg tatagaaaag tgtcacttgt ctatagtaaa
      121 cacaccttta ctttatttct tacaatatac ctaactgttg ccgttgccgg ctctataatt
      181 agtgttgtgc gttttggtgg cactttgcca gtgctaccaa caggcactgt cgtttggtac
      241 ctgattggcg aaggtttgtt tataccaatg tcgtggtact tttcgtaccg attattatct
      301 attatcggcg cttcttctat ggcggttgcc cagtctttta tttttttgac aaccgccact
      361 ttaggtatct tattgcttga cgagccactg acggtaggat tatttgttgg ggctttgcta
      421 ttgctttctg gtgtatattt agcccttagt gtgaatggcc ggcacgttaa aaaaaataat
      481 gttactctaa ccaaaaaact ctatttatta tctatgagcg cgttgctttt atctattggc
      541 ttactatttg aaaaattggc aatcaacagc attggtgtgt gggattatgc tatgtttggg
      601 tgggctgcac aaggagctgg cgcagtgatt ctatttagta tttttggccg gcaggaactc
      661 gggctgcagc gcaccgctag attctggcgc tacgcccttg tagctggttt actgactaca
      721 cttacggggt cactgtttat ttatgcgctt agtacaagca tgctgagttc agtggtgctc
      781 gcttctactg caaagattac tcttacgtct gtgctggctt tttggctttt aaaagaacgt
      841 acatatttag gcaaacgaat cgctgctgtg gcgctcgctg tatgtggatt actagcaata
      901 tttgcatagt aatagggtat aataacaagc ataacgctaa aggttgcaaa catgggacat
      961 acaatacaac aaataagaca aaactgtact aaagcacgcg aaacaatggc caatgcacga
     1021 gtccagaaat ttgctgtagg tgcgtttaat atagataacc aagaaacgct tattgcggtt
     1081 gcaaaagctg ctcaaaaact acaagcgcca cttatggtag aggtcagcca tggcgaagta
     1141 gaagcaatgg ggctcatgaa cgtgcgcgat atggtagata attacaagca agaatatggc
     1201 atagaaatgt atattaacct agatcatagc ccatctgtag aaactgccaa agcgggtata
     1261 gatgccggat ttgaatttat tcatatagat gttagccaag ctaatcacaa cgcaacagat
     1321 aatgaaataa taattgctac tagggaagtt gttcgttatg caaccttcac tggtgcaatt
     1381 gtagaaagcg aaccccatta ctttggtggc agcagtaacg tgcacaaaga ggcttttgat
     1441 tacgctgata ttaaaaaaac atttagtaca ccacgtggtg ccgaaaagta atggggttcg
     1501 ctttctacaa tt
//
LOCUS       DAOPLS010000199         1497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_101189_length_1497_cov_2.966713, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000199 DAOPLS010000000
VERSION     DAOPLS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101189_length_1497_cov_2.966713"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..539)
                     /locus_tag="PKV52_04035"
     CDS             complement(78..539)
                     /locus_tag="PKV52_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPD99412.1"
                     /translation="MQPKKPRRKSAKQQIEELTEDLQRLQAEFINFKRRSEEDRIKAV
                     SFGKEQAFEKLLPILDNLERALAQPPADIAEHPWVKGVWAANKQLEAELSAAGLQKYG
                     AVGEPFNPEIHDAVSMIDGDGEKEVIAAVAQTGYMLDGAIIRHAVVQVTKQ"
     gene            complement(556..1278)
                     /locus_tag="PKV52_04040"
     CDS             complement(556..1278)
                     /locus_tag="PKV52_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPD99413.1"
                     /translation="MTERQANILQAIVEQYAEVAVPVGSVLLAKTFGVSSATIRAEMA
                     RLEDMGYVEHPYTSAGRVPTDKGYRYYVNAVTEQAAIPHESLNGSTKIDRNARAIDVR
                     VRSAGKAAQTIKTAVDSLVDITHNLSIATIGDQLYMSGISHLFSQPEFADGTAVHEVA
                     KLIDNLEPWLQEASPNEPLSVYIGAENPIGKASGCSLIISKFRSPYSDRSYIGVVGPT
                     RQSYKQVMGLVRHAGQLLEKVL"
BASE COUNT          377 a          352 c          299 g          469 t
ORIGIN      
        1 cgattctagg ctttgttcat actgtggggt gtgcactgct aatacatcag tatattgatg
       61 cacggactca ctcatggcta ttgcttggtt acctgcacaa cagcatggcg aataattgca
      121 ccgtccaaca tatagcctgt ttgggctact gcagcaataa cttccttctc gccatcacca
      181 tcgatcatac taacggcatc gtgaatttct gggttaaatg gctctcctac cgctccatat
      241 ttttgtagac ctgcagcact tagctcggct tctagctgct tgttagcagc ccatacgcct
      301 tttacccaag ggtgttcagc aatgtcagcc ggcggctgag caagagcgcg ttcgagatta
      361 tcaagtatag gtagtaattt ctcaaatgct tgctccttac caaagcttac ggctttaata
      421 cgatcttctt cagaacgccg cttgaaatta ataaactctg cttgtaatcg ctgcaaatct
      481 tcagtgagtt cttctatttg ttgttttgcg ctttttctac gtggtttctt tggttgcata
      541 tactacatac tttccttaca atactttttc taataattgg ccagcatggc gaacgaggcc
      601 cattacttgt ttgtaacttt ggcgggttgg acctacaaca cctatatagc tcctatcgct
      661 atatgggctt ctaaacttgc taattattag gctacagcca ctcgccttgc ctatgggatt
      721 ctcagcacca atatatacac taagcggttc attgggcgat gcttcctgga gccatggttc
      781 aagattatct ataagttttg cgacttcatg cacggcagtt ccatcggcaa attctggctg
      841 actaaacaag tgggatatac cactcatata gagctgatca ccaatggtag cgatagacaa
      901 attatgggta atatctacta agctatctac tgctgtttta attgtttgag cagcttttcc
      961 tgcactacga actcgcacat caatcgctct cgcattacga tcgattttag tagaaccatt
     1021 taatgattcg tgcggaatcg cagcctgttc ggttaccgca ttaacataat accgataccc
     1081 cttatctgta ggtactcgcc cagcagacgt atacgggtgc tctacgtagc ccatatcttc
     1141 taggcgagcc atttctgccc gaatagtcgc agatgataca ccaaaagttt ttgccaacag
     1201 tacagaacct accggaacgg caacttctgc atattgctct actatagcct ggaggatatt
     1261 tgcttgtctt tctgtcataa aatatagtaa ctacgtttta ctgatggtag tatagcgtat
     1321 ttagcactcg ttagtcaaga gtgctaaata ctggttgtat ctgtgtcctg tatatgaaca
     1381 aattagcgca aatatgcgct taaatctgct agcagctgct cggcatgaac gtgcgcattg
     1441 gcttgttcta actctccaaa atctcctgtt gtaatatgct gatgaagagc atctaca
//
LOCUS       DAOPLS010000200         1495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_101400_length_1495_cov_2.349306, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000200 DAOPLS010000000
VERSION     DAOPLS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101400_length_1495_cov_2.349306"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKV52_04045"
     CDS             <1..216
                     /locus_tag="PKV52_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99414.1"
                     /translation="KKFSFEVVALIANIAGTTSIVTQTVTPIYRDDTNFNCQLAVSGN
                     ELLAQVRDTGVSGDTVLWHAVFYTSEV"
     gene            220..>1495
                     /locus_tag="PKV52_04050"
     CDS             220..>1495
                     /locus_tag="PKV52_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99415.1"
                     /translation="MSFHAQLTLDDLHPPFRWIWADEASRVAQSVTADDVAKMGWRQD
                     NNAIYALVDTAPTWAQVGGGSTPALGDLTDVTLVGLVDGELIKYNSGTGEWENGTMAL
                     ADLSDIDLTGVADGSILYYSGGTWYVMDGNVHLVSDSATATASTAVTLGHNTTGTPAA
                     GFGTALDVEAEDDTAPNQPIGSIVFSWVDPTNGATYSRVALYTMADSAKVLRAVFEPP
                     NIVPVVAGNARGAGAVELQASRYNAVQVASGDGSGILSGDSNTADSDYSLIVAGSGNT
                     SNGAFASVMNGEQCTAIADYSTILAGVWAETASRAQIALSGFYFAAPSDALITLAVPV
                     GREVAHGDTNWYTLFTNGTTLSPAYVYVPDNVAITFTGKVIGTDSGLTKVQSYTFSGA
                     ASCPGGVLTIHASNVTSIFETDSNFAVQIVSSG"
BASE COUNT          399 a          290 c          395 g          411 t
ORIGIN      
        1 aaaaagttca gctttgaggt tgtagcattg attgctaata tagctggtac tacaagtatt
       61 gtcacacaaa cagtaacccc aatttaccgc gatgatacca attttaactg tcaattggcg
      121 gttagtggca atgagttact agcacaagta cgtgatacgg gcgttagtgg tgatactgtt
      181 ttgtggcacg ctgtattcta tacaagtgag gtataataaa tgagttttca tgcgcaacta
      241 acattagacg atctacaccc accgtttcgc tggatttggg ccgatgaagc aagtcgagtc
      301 gcgcaaagtg taactgccga tgacgttgca aaaatgggtt ggcgacaaga caacaatgca
      361 atttatgcgc tagttgatac tgcgccaacg tgggcacaag ttggcggcgg cagcacaccg
      421 gctttgggcg atctgacaga tgtaacatta gtcggtttag ttgatggcga actaatcaaa
      481 tacaacagtg gcacaggtga atgggaaaat ggtacaatgg cattggcaga cctatcagat
      541 attgatctaa ctggtgttgc tgatggtagt atcttatatt acagcggtgg cacttggtat
      601 gtcatggatg ggaacgtgca tttggtgtca gatagtgcca ctgcaacggc aagtacagcc
      661 gtgacattag gccataatac cacaggtacg ccagcagcag gtttcggcac agcgttggat
      721 gttgaagcgg aagatgatac tgcgccaaat cagcccatcg gtagcattgt ttttagttgg
      781 gttgatccga ctaatggggc aacctatagc cgggttgcgc tatatacaat ggcagacagt
      841 gccaaagtgc tacgagcggt ttttgaacca cctaacattg tgccagtagt ggcgggtaat
      901 gcgcgtggtg ctggtgctgt tgaactacag gctagtcgtt ataacgctgt acaagttgcc
      961 agcggtgacg gtagtggtat tttgtcaggc gatagtaata ctgctgatag tgactacagt
     1021 ttgattgtag ccggatcggg caataccagc aatggggctt ttgccagcgt tatgaacggc
     1081 gaacaatgta ctgccattgc agactacagc acgattttag cgggtgtttg ggcagaaacg
     1141 gcgtcacggg cacaaattgc acttagtggt ttttatttcg cagccccgtc agatgctttg
     1201 ataacactag ctgtacctgt tgggcgtgag gttgcacacg gtgatacaaa ttggtatacc
     1261 ttattcacaa atggtacaac actttctcca gcatatgtct atgtacctga taatgtagca
     1321 attacgttta ctggtaaagt gattggcact gatagtggtt tgacgaaggt acaaagctac
     1381 acttttagcg gtgctgcaag ttgtcctggc ggtgtcttaa caattcatgc aagcaatgtt
     1441 acaagcattt ttgaaactga tagtaatttt gctgtacaaa ttgtatcatc tggcg
//
LOCUS       DAOPLS010000201         1470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_104054_length_1470_cov_2.306714, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000201 DAOPLS010000000
VERSION     DAOPLS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104054_length_1470_cov_2.306714"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1470)
                     /locus_tag="PKV52_04055"
     CDS             complement(<1..>1470)
                     /locus_tag="PKV52_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99416.1"
                     /translation="WKQKVDLYGKGTIDCSSRIQIDKAINPNIFNPLVNRAVLYEAGG
                     NNCADENYHQGAIDLQNEDESVTKAAQNINWGNEPLGCPKFGNIPNNANKKDLNYNCP
                     NGGAVIKGVYDKNEKIDTPRLASANASACGDVAGAFGWLICEPAEKIGDFTMWFEKQL
                     YTLLHINELPLAADGGVYQAWASIRTVATTVLVLVALVAIASQIFNFDFISAYTIKKI
                     IPKIVIATILIFTSYYLAAMGIAFVNALGDGVNALILAPFPDLMDAYNQNETISFVLS
                     NISGIGGGEAGVAGGIIVGAGIAAIGGAGLFGLLIPIILVIGAVVVAFITLVLRKVFI
                     LALVLIMPLAIVAWILPGTQKWFDSWWKLFSKLLLMYPLVIGLLAVGKVAAYLIATGT
                     ESVTAAALPQVWNVLAVNGQASIVILMVLIAYFGPYYFIPSMFKTAGGVFGKITSGMQ
                     SFGSKYARKGGEASSKWVGDRAASRYRPGAKGARKFIGNS"
BASE COUNT          431 a          350 c          264 g          425 t
ORIGIN      
        1 agagtttcca atgaactttc gtgctccctt tgcacctggc ctatatctcg aggctgctct
       61 atcaccaacc cacttacttg acgcttcgcc gcctttacgt gcgtatttac ttccaaagct
      121 ttgcatgcca ctggtgattt ttccaaacac accacctgct gttttaaaca tacttggaat
      181 aaaatagtag gggccaaaat aagctatcag caccattaga ataactatag atgcctgccc
      241 attcacagcc aaaacattcc atacttgtgg tagtgcagca gctgtaactg attctgtgcc
      301 ggtagctatt aaatatgcag ctaccttacc cactgcgagc aagccgatga ctaatgggta
      361 cataaggagt agtttagaaa agagcttcca ccagctatca aaccattttt gcgtaccagg
      421 cagtatccat gccacaattg caaggggcat aatgagtacg agtgctaata taaatacttt
      481 tcttagtaca agtgtaataa acgcgacaac caccgcgcct attacaagta taattggtat
      541 gagcaacccg aacaatccag ccccacctat tgctgctata cctgcgccta caattatgcc
      601 acccgcaacc ccagcttctc caccaccaat accagaaata ttgctaagta caaaagatat
      661 cgtttcgttt tgattataag cgtccattaa atcaggaaaa ggcgccaaaa taagagcatt
      721 tacgccatca ccaagcgcat tcacaaacgc aatacccatt gctgcaagat aatacgatgt
      781 gaaaatcaaa atggtagcga taactatttt gggtattatt tttttaatag tataggcact
      841 aataaaatca aaattaaata tttgggaggc aattgcaaca agcgccacga gcaccaatac
      901 tgttgttgcc acagttcgaa tacttgccca agcttggtaa acaccaccat cagcagccaa
      961 gggcagttca tttatgtgca acaaagtata cagttgcttt tcgaaccaca tggtaaaatc
     1021 tccaattttc tcggcaggtt cgcatattaa ccacccaaaa gcacctgcta catcaccaca
     1081 agcacttgcg ttagcacttg ctagtcgtgg tgtatcaatt ttttcatttt tgtcatatac
     1141 gccttttata accgcaccac cgtttgggca gttataattg agatcttttt tattagcgtt
     1201 atttggtata ttaccgaatt tagggcagcc gagcggttcg ttaccccagt taatattttg
     1261 ggctgccttt gtaactgatt catcttcatt ctgtagatca attgcacctt gatgatagtt
     1321 ttcgtccgca cagttgtttc ctccagcttc gtagagcaca gctctattta cgagagggtt
     1381 aaaaatattt ggattgatag ctttatctat ctgtatacgt gaagaacagt ctatagtacc
     1441 cttgccgtaa aggtcgacct tttgcttcca
//
LOCUS       DAOPLS010000202         1469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_104127_length_1469_cov_3.490099, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000202 DAOPLS010000000
VERSION     DAOPLS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104127_length_1469_cov_3.490099"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKV52_04060"
     CDS             <1..456
                     /locus_tag="PKV52_04060"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPD99417.1"
                     /translation="VVKWTNQTLPEDKPRHLLGMGAQPVDLFLGIEYGVDTFDCVAPT
                     RQARNGSIYTKDGRINITNSKFAKDFDPIENDCDCYSCNGYSRAYINHLFKSDEILGA
                     TLASIHNERFVVRTVDEIRRSIEDNTFFELKKAFLHRYYGNAVPNFAGV"
     gene            complement(469..615)
                     /locus_tag="PKV52_04065"
     CDS             complement(469..615)
                     /locus_tag="PKV52_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99418.1"
                     /translation="MVQLIATVYAIKIVTEIALSPLTMKIITYLKKKERLDTFEEPKL
                     GLYN"
     gene            complement(600..1136)
                     /locus_tag="PKV52_04070"
     CDS             complement(600..1136)
                     /locus_tag="PKV52_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPD99419.1"
                     /translation="MPTKKTFIYYEVLVALLITTLIVSNIASVKLVQLGNLVFDAGTI
                     LFPLAYIIGDIITEIYGFRRMRRLLYIGLAMLALTTVVFAIVQVLPSPADWQNQAAYT
                     VILGVVWRIVFASMVAFFVGELLNSYILAKLKIKTKGKKLWNRLVSSSAIGSLIDTII
                     FSVLAFGGTISGKQWCNL"
BASE COUNT          495 a          288 c          264 g          422 t
ORIGIN      
        1 gttgttaagt ggaccaacca aacattgcca gaagacaagc cacgtcactt actaggaatg
       61 ggtgcacaac ccgtagacct gtttttgggt atcgagtatg gcgtagatac atttgactgt
      121 gttgcaccaa cccgccaagc ccgcaatggg tccatctata ccaaagatgg ccgcataaac
      181 ataaccaaca gcaagtttgc caaagacttt gatccaatag aaaatgattg tgattgttat
      241 agttgcaatg gctacagcag agcatatata aaccatttat ttaaaagcga cgaaatatta
      301 ggcgccacac tggctagcat tcataacgaa cgcttcgtgg tgcgtactgt agacgaaatc
      361 cgccgtagta tagaagataa tacatttttt gagctaaaaa aagcattctt acaccgctac
      421 tacggcaatg ctgtgccgaa ctttgcgggt gtgtaattct agtagtaact agttatataa
      481 cccgagtttg ggttcttcaa aggtgtctaa acgttctttt ttctttaggt aggtaataat
      541 cttcatagta aggggtgaca gggcaatttc tgttactatt tttatcgcat aaacagttgc
      601 tataagttgc accattgttt gccactaatg gtacccccaa atgccagtac actaaaaata
      661 atggtatcta tcaagctacc aatggcagac gaacttacta aacgattcca taattttttg
      721 ccctttgttt ttatttttag ttttgccaaa atgtagctat tcagtagctc gccaacaaag
      781 aacgctacca tactggcaaa aacgattcgc catacaacac ctaatatcac cgtatacgca
      841 gcttggtttt gccagtctgc tggacttggt aatacctgaa caatagcaaa tactacagtt
      901 gtaagcgcga gcatcgcaag gcctatgtag agtaaccgac gcatacgcct aaagccataa
      961 atttctgtaa ttatatcacc aatgatatat gcaagcggga ataatattgt accagcatca
     1021 aaaactaggt ttccaagctg tacaagcttt acacttgcta tgttactcac gatgagcgtc
     1081 gtgataagta gcgcgactaa tacttcgtaa tagataaatg tttttttagt aggcatacaa
     1141 aaaataagta gttaattaat ttttacgatt ttgtatgtca cctaaaaaac gtatatattc
     1201 aattatacca aactatttac actaagctaa taagatataa tgcaacggca attgccaaga
     1261 aaatatttat ataaaagaac acaatcagta gtttaccaat aaaagtatcc ttgaatgtac
     1321 tttgctgaat cattctaata catagtacta agaatagtgg taaaacgagt aatgtaaacg
     1381 ctccaaaaaa ttgcatataa gctcctttgt taccctagta tattgataga tcgcgcaata
     1441 agcaatgcca acgtaaatac agaaacaag
//
LOCUS       DAOPLS010000203         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_104216_length_1468_cov_4.182590, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000203 DAOPLS010000000
VERSION     DAOPLS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104216_length_1468_cov_4.182590"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKV52_04075"
     CDS             <1..384
                     /locus_tag="PKV52_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99420.1"
                     /translation="VIVDIVFGFGLTDGFFAKRGDDTLHPAVLIMTATPDQVVVPPTS
                     TPLPTLTPLPTSTPLPTLTPTPLPTPASVFGTCGVDMSCPATVAPVCGINMSCPDDRS
                     DFEILKDAALDRVGAIISDTIDVLK"
     gene            complement(414..881)
                     /locus_tag="PKV52_04080"
     CDS             complement(414..881)
                     /locus_tag="PKV52_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99421.1"
                     /translation="MANLPGSALMLKIDNVRPTVGGERIVVNIDGGLATVPNTDDDGT
                     PTGETVPTLIVQTPPAALDDTPITVNLSSYGRGSIALLWFTARNITAENNNYDFYTGS
                     NTAGNRVRVITPADSDYYTGLVAVQLSNNSFTFRPTASALAEAVYTVELIAML"
     gene            complement(903..>1468)
                     /locus_tag="PKV52_04085"
     CDS             complement(903..>1468)
                     /locus_tag="PKV52_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HPD99422.1"
                     /translation="DPMLMPPRRKGDPGRNWADTLVVAMDGAIKRDSFALVAVSRHPN
                     NRNDVAVRLVHEFKPINGKIDFRTVEDVLRSWCDNYNIVTIVYDVYQLYDMTERLRKE
                     GIAWFQEFSQQRQRTLSDNLLYELIVQERIRHSNQAELTDHINNAGHRYDSAEMKRRI
                     VKIRESRKIDLVIATGMAAWECLRLNL"
BASE COUNT          387 a          333 c          312 g          436 t
ORIGIN      
        1 gtaattgtag atattgtttt tgggtttggg cttacggatg gtttttttgc caaacgtggt
       61 gatgacacct tgcaccctgc tgtgctgatt atgacagcga cacccgatca agttgttgtg
      121 ccaccaacaa gtaccccctt accgacttta accccgttgc ccacaagtac ccctttacca
      181 acactaaccc caacaccctt gccaacacca gcaagtgttt ttggcacatg cggggtagat
      241 atgtcttgcc cagcaacagt tgcaccagtt tgtggtataa acatgtcatg tcctgacgat
      301 agaagtgatt ttgagatact caaagatgcg gcattagacc gtgttggggc aattataagc
      361 gatacaattg acgtactgaa ataaaaaagg gggctagatg cccccttttt ttattagagc
      421 atcgcgatta gctcaacagt gtaaacagct tccgcaagtg ccgatgcagt tggtctaaaa
      481 gtaaaactgt tattgcttaa ttgtacagca actaaacctg tatagtaatc actatcagct
      541 ggtgtaataa cacgtacacg attccccgca gtgtttgaac ctgtgtagaa atcatagttg
      601 ttgttttccg ctgtaatgtt acgtgcggta aaccacaaca atgcaatact accgcgccca
      661 taactcgaca agttgacagt tatgggtgtg tcatctaatg cggcaggtgg tgtttgtaca
      721 atcaaggttg gcacggtttc ccctgtaggt gtaccatcat catctgtgtt gggcactgtt
      781 gctagtcctc cgtcaatatt tactacaatt cgttcgccac caacggttgg gcgcacgtta
      841 tcaattttaa gcattaatgc gctgcctggt aagtttgcca taatgtcatt ataatttgcg
      901 gtttatagat ttaaccgcaa acattcccat gctgccatgc cggttgcgat aactaagtca
      961 attttgcggc tttcacgtat cttaacaatg cgtcgtttca tttcagcact atcgtaacga
     1021 tgtccggcat tattgatatg atcagttaat tctgcttggt tactatggcg aatgcgttcc
     1081 tgtacgatta gctcgtatag taagttatca gatagtgtac gttggcgctg ctgtgagaat
     1141 tcctgaaacc aagctatgcc ttccttacgc aaacgctcgg tcatgtcata tagttgatat
     1201 acgtcataga caattgtgac aatgttataa ttatcacacc aactacgcaa tacatcttca
     1261 accgtgcgaa agtcaatttt accgttaatg ggtttgaatt cgtgtacaag ccgtactgcc
     1321 acatcattgc gattgttagg gtgtctgcta acagcaacga gtgcaaacga gtcacgttta
     1381 atagcaccat ccattgccac aaccagcgta tctgcccaat tacggccagg atcaccttta
     1441 cgacgtggtg gcattaacat tggatcgt
//
LOCUS       DAOPLS010000204         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_104223_length_1468_cov_3.707006, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000204 DAOPLS010000000
VERSION     DAOPLS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104223_length_1468_cov_3.707006"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1198
                     /locus_tag="PKV52_04090"
     CDS             182..1198
                     /locus_tag="PKV52_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99423.1"
                     /translation="MKIHKHIEIVSSSLPVLSSMSERSRNAIQKTLQKHYLHVGITLI
                     DTPNDLKKLIKRKPDLVFLGMKFVPEVSSMVHNEPIKYWISEHLDIHGIPYTGSSKRA
                     HIQELNKDIAKDLMLEADISTAKYYVGKRGMPATWQNNNLKYPVFIKPANRGGGIGID
                     DFSIAYGQAAINAKVRSVTNSLQSDVLVEEFLSGQEYSVAIMRKPDSDGYDSLAIELS
                     TQQEGTDVSFISSNIKEANSEVVLTVDDLAVNQQVSQLALDAFVALGARDYGRIDIRL
                     DEHGVPHFLEANLLPSLIEDYGSFPKACKLNAHIDYESMILNIVDLAFTRTAKTPELV
                     LQNI"
     gene            complement(1247..>1468)
                     /locus_tag="PKV52_04095"
     CDS             complement(1247..>1468)
                     /locus_tag="PKV52_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPD99424.1"
                     /translation="CENNDKFAIQRKLPPTVPGDIIVIHDTGAHGHSMGFNYNGMLRS
                     AELLLETSGTVRQIRRAETIDDYFATLAF"
BASE COUNT          481 a          279 c          288 g          420 t
ORIGIN      
        1 aatagaataa aatatcggta tatattacta catgtggtat aataatatgt actagttgtt
       61 acaagcaaca tctctatttt tgttagttag ctaaaactta ttatcatttt gtgagatacg
      121 gtaacttgtt acgtattgtt aattaatggc gtgctattgc aggtctggtt gggacaaaaa
      181 catgaagata cacaaacaca tagaaatagt cagctcttcg ttacctgtac taagttctat
      241 gagtgaacgc tcaagaaatg ctatacaaaa aacattgcaa aaacattacc tgcacgtagg
      301 tattaccctc attgatacac caaacgatct aaaaaaactg attaaacgca aaccagattt
      361 agttttttta ggcatgaaat ttgtaccaga agtgtctagt atggtgcata acgaacctat
      421 aaagtactgg ataagtgaac atctagatat acatggcatt ccctacacag gctctagcaa
      481 gcgggcacat atacaagaac taaacaaaga catcgccaag gatctaatgc tagaagcaga
      541 catttctaca gccaaatatt acgttggcaa aagaggcatg ccagccacat ggcagaataa
      601 taatttaaaa tacccagtgt ttataaaacc tgctaacagg ggcggaggta tcggtataga
      661 cgatttctct attgcctacg gccaagcggc cattaatgca aaagttcgta gcgttaccaa
      721 tagcctacag tctgatgtac tggtagaaga atttttatca ggccaagaat attcagttgc
      781 tattatgcgc aagcctgatt cagatgggta tgattctttg gcaatagaac tgagtacaca
      841 gcaagaaggt actgatgtta gctttattag cagtaatata aaagaggcaa attcagaagt
      901 tgtacttaca gtagatgacc ttgcagttaa tcaacaagtt tcccagcttg cactcgatgc
      961 ctttgttgcg ctcggtgcta gggactatgg tcgtatagat attcggctag atgaacacgg
     1021 cgtaccacat tttctagaag caaatttact ccccagcctc atagaagatt atggttcgtt
     1081 tcctaaggcg tgcaagttaa atgcccacat agattacgaa tccatgatac tgaatattgt
     1141 tgatttagcg tttacgagga ctgcaaaaac accagaactt gttctgcaaa atatatagtt
     1201 tttttgccgt cgttgcacta attggcaagt ataaatactt agcagactaa aatgctaggg
     1261 tagcgaagta atcgtcaatc gtctctgctc gccgaatttg ccgtactgta ccactagttt
     1321 caagaagtag ctctgcagat cgcaacatac cattgtaatt aaagcccata ctgtgaccat
     1381 gcgcacccgt atcatgaatc acgatgatat caccaggaac agtaggtggt agtttacgtt
     1441 gaattgcaaa tttatcgttg ttttcaca
//
LOCUS       DAOPLS010000205         1459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_105238_length_1459_cov_1.586895, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000205 DAOPLS010000000
VERSION     DAOPLS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105238_length_1459_cov_1.586895"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKV52_04100"
     CDS             complement(<1..423)
                     /locus_tag="PKV52_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPD99425.1"
                     /translation="MIELKDFQQKAVDSLLSTFRRLTSRREPSVSIFKAPTGSGKTIM
                     VAEFLKRLADEDLPENYVFVWASLYDLHSQSKAKLTDYLRDSRYKLMTLSDVTEDSLQ
                     NDSVLFVNWHSLTTTKDNGGSERDWSNVYVRDQEGGSII"
     gene            complement(420..>1459)
                     /locus_tag="PKV52_04105"
     CDS             complement(420..>1459)
                     /locus_tag="PKV52_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD99426.1"
                     /translation="RANQLVSRGLSSDEIHKELLELVKYPRFYDLDHFTFADKRGVYQ
                     TDNPGGVKNGNTKSVVMHPVTNKPCTVPDGGWRYKDEEIKRLIKEGLFEFGKDETIVP
                     RPKRYLKDYMEQTPKSSIFFDSQSSTKMLKKMGIAFDFPKATELIEYLISMYPNNKAV
                     TVLDFFAGSGTTGHAIIKLNKKDNGSRKFILCTNNEGGIAENVTYKRIANVIDDTPAN
                     LRYFKTSFVDKAENTDQTRVALVARATDMIKVRENTFETVVEEDLFKAYEGADTYSVI
                     AFDPSVIDKAKTEIAKLSDDKAVRVYVFSLANDSYVSDFADLDRQVELCPIPESILEV
                     YKRIFDQKGTK"
BASE COUNT          370 a          312 c          292 g          485 t
ORIGIN      
        1 gatgatactg ccaccttctt gatcacgcac gtagacgtta gaccaatcac gttcgctacc
       61 accgttgtct ttggtggttg tcagactatg ccagtttacg aacagtacac tatcattttg
      121 taggctatct tcggtaacgt ccgaaagtgt cattagcttg taacggctat cacgtaagta
      181 gtctgtcagt ttggctttgc tttggctgtg caggtcgtat agtgaagccc atacaaatac
      241 gtagttttcg ggtaagtctt cgtcagcaag acgcttcaaa aactcggcta ccataatagt
      301 tttgcctgat ccagtcggtg ctttgaatat acttacagac ggttctctac ggcttgttaa
      361 tctgcggaac gtagaaagta gactatctac cgctttttgt tgaaaatctt ttagctcaat
      421 cattttgtgc ctttctggtc aaatatccgc ttatacactt ctaatatgct ttcggggatc
      481 gggcatagtt caacttgacg gtccagatca gcaaaatcac ttacgtagct gtcgtttgct
      541 aggctaaata cgtagacacg cacggcttta tcgtcggata acttggctat ctcggtttta
      601 gctttgtcga taacagaagg gtcaaacgct attacagaat acgtatcagc cccttcgtac
      661 gccttgaata agtcttcttc gactaccgtt tcaaacgtat tctcacgtac cttgatcata
      721 tcggtggcac gtgctactag tgcgacacgg gtttgatcgg tgttttctgc tttatctacg
      781 aagctagtct tgaaataccg aagattggca ggtgtgtcgt ctatgacatt tgctatccgt
      841 ttgtaagtta cattctcggc aattccacct tcattatttg tacataaaat aaacttgcga
      901 ctaccgttat cttttttgtt cagtttgatt atggcgtgtc cagttgtgcc agaaccagca
      961 aaaaagtcca gaaccgtgac agctttatta ttggggtaca ttgaaattag gtattctatc
     1021 agctcggttg cttttggaaa gtcaaaggct atacccattt ttttcaacat ctttgttgat
     1081 gattgcgagt caaaaaagat tgaagattta ggtgtctgct ccatataatc tttcaaatat
     1141 cgctttgggc gtggcacaat agtttcgtct ttaccaaact caaacaaacc ttctttaatc
     1201 aggcgtttaa tctcttcgtc tttgtaacgc caaccgccgt cgggtacagt gcaaggttta
     1261 tttgttactg ggtgcataac aacagattta gtgttgccat tttttacacc acctgggttg
     1321 tcagtttgat aaacgcccct tttatcagca aaagtaaaat gatctaaatc ataaaatctc
     1381 ggatacttta ccagctccaa taattctttg tgtatctcgt ctgaacttaa accacgtgat
     1441 acaagttgat ttgctcgtt
//
LOCUS       DAOPLS010000206         1445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_106658_length_1445_cov_2.727338, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000206 DAOPLS010000000
VERSION     DAOPLS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106658_length_1445_cov_2.727338"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PKV52_04110"
     CDS             complement(<1..1342)
                     /locus_tag="PKV52_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HPD99427.1"
                     /translation="MKKIESALLLYPHQLFATEHLPKVDIIYVIEDPLFFGTDQEYPV
                     AFHKQKLVLHRASMRRYTEEQLWAHEIDVEYIELADVVSSGEVLSHAQKAGASLVYMF
                     DPSDSRLEKRLQDALSTNIVSPFELRVLPTPMFMLRRGEVEDFFTNKSNHSFAPFYQW
                     QRERFNILLDKKYKPVGGTWMLDDHSTKSDTTQPAPGFASFGDNNYVKEAISWVQKRF
                     ADNPGSLESFVWPTNHQEATMWLQDFLTHRLEHYAQFEHALDSDAMVLYHSGASALIN
                     TGLLTPTQIVQAATAQQQKAPVPLPSLEHFLRYVIGWREYVRGLYVTQDDELRARNTF
                     EHTNTLSSAWWDGSTGLPPLDDVIQKALNHAYTYGSERTHILASSMLLCDISPNEAYQ
                     WNMSMFIDAYDWVVVPNVYGIGQCDDCGGMVPETCLMTSNGLISNSNYQKEDWCD"
BASE COUNT          385 a          313 c          309 g          438 t
ORIGIN      
        1 tatcgcacca atcttctttt tggtaattac tgttagaaat caaaccattg ctcgtcataa
       61 ggcaggtttc tggcaccatg ccaccacaat catcacattg gccaatgcca tacacatttg
      121 gtaccacaac ccaatcgtag gcatctataa acatgctcat attccattgg tatgcttcgt
      181 ttggagaaat atcacatagc agcatgctac ttgcaagtat atgggtacgt tcggagccgt
      241 aagtgtaggc atggtttaga gctttttgta taacgtcatc tagtggaggt agcccggttg
      301 aaccgtccca ccacgcgcta ctgagtgtat tggtatgttc aaacgtattg cgcgcacgta
      361 gttcatcatc ttgtgttaca tataggccac gtacgtattc gcgccagcca ataacatacc
      421 gaagaaaatg ctctaaactt ggcaatggta cgggtgcttt ttgttgctgt gccgttgccg
      481 cctgaacaat ctgcgttgga gttaatagac cggtgttaat gagtgcagaa gccccactgt
      541 gatagagcac catagcgtcg ctatctaggg cgtgttcaaa ttgtgcgtaa tgttcaagac
      601 gatgtgtcag aaaatcttga agccacatag tagcttcttg atggttggtt ggccacacaa
      661 aagattctaa cgaaccgggg ttatctgcaa aacgcttttg cacccaagag atagcctctt
      721 ttacatagtt gttatcgcca aagctcgcga aaccgggggc aggctgggta gtatcactct
      781 ttgtagagtg atcatctagc atccacgtac caccgactgg tttatatttt ttgtctagta
      841 atatattaaa acgttcgcgt tgccactgat aaaatggggc aaaagaatga tttgacttat
      901 tagtaaagaa atcttctact tcgccccttc tgagcatgaa cattggcgta ggcaaaaccc
      961 gcagttcaaa cggtgacact atgtttgtag atagcgcatc ttggagacgc ttttctaagc
     1021 ggctatcgct agggtcaaac atatagacaa ggctagcccc agctttttgg gcgtgtgaaa
     1081 gtacttcgcc tgaacttacg acatcggcaa gttctatgta ttctacgtct atttcgtgtg
     1141 cccacaattg ctcttctgtg tacctgcgca tgcttgcacg gtgcaatact aacttttgct
     1201 tatgaaacgc cacagggtat tcttggtctg taccaaaaaa cagtggatct tctataacat
     1261 atatgatatc tacttttggt aaatgctcag tggcaaatag ttgatgtgga tacaataaca
     1321 aagctgattc tatttttttc atacgctcat tctttttatg cttttatata cacagtatag
     1381 tagttttgcc gctactaaca aattaaatca ctgtttatgt agccttagat attgctagct
     1441 cggcc
//
LOCUS       DAOPLS010000207         1442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_107003_length_1442_cov_2.728190, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000207 DAOPLS010000000
VERSION     DAOPLS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107003_length_1442_cov_2.728190"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKV52_04115"
     CDS             complement(<1..354)
                     /locus_tag="PKV52_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPD99428.1"
                     /translation="MIPIALILDNIRSTYNVGSILRTADCFGVQEIFFSGYTPYPISK
                     ADRRMPHIAKKLHEQIAKTALGAESTLPFSVFDTIATAIDAAQSAGYKIIGIEQDTNS
                     IPLDSYSFTQPSALIL"
     gene            417..>1442
                     /locus_tag="PKV52_04120"
     CDS             417..>1442
                     /locus_tag="PKV52_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPD99429.1"
                     /translation="MQDENQARRLDEQTAQRQAHIVGVPYFDTSNVDIAIYKEAVSIE
                     DIKKHKFVPLYVDANTMRFGITNTTPQAILQKLRQDHLDQRIEFYLISDSGYRDLLRK
                     YDPPKQVIYDDINIGNTTNTEQVATISHTLGSVRADDMLAYVVKQAYQLHASDIHLET
                     DRNEVRIRFRVDGVLHPIALLSIEKYRQLVSSLAVAANISTSSADAQTGHINKNYMLA
                     DGSNVEVNLRVETVPTVHGMDAVLRLFTLNTDLMQLHKLNLQPDELHIVKEVISHPNG
                     LVLIVGPTGSGKTTTLYSILNELNSTERKLITLEDPVEYNIKGVTQIPVDSRQVKTGF
                     AEKFRAVL"
BASE COUNT          454 a          285 c          303 g          400 t
ORIGIN      
        1 aaggataaga gcacttggct gggtgaagct gtacgaatct aacggtatac tgtttgtatc
       61 ttgttctata ccaataatct tatacccggc tgattgggct gcgtcgatgg ctgttgctat
      121 cgtatcaaat acgctaaaag gcagtgtaga ttcagccccg agtgctgttt ttgcaatttg
      181 ctcatgaagt tttttagcta tatgaggcat gcgccggtct gccttactga taggatacgg
      241 agtatagccg ctaaagaaga tttcttgtac gccaaagcaa tcggctgtac ggagtatgct
      301 cccaacattg taggtactcc gaatattatc caatataagg gcaataggga tcatgtattc
      361 tatagtaaca aattatatat agcctatact ccttatgctt gctactatgt aagggtatgc
      421 aagacgaaaa ccaagcaaga cgtctagacg aacagactgc acagcgccaa gcacatatcg
      481 ttggggtccc atattttgat acttcaaatg tagatattgc aatttataaa gaagcagtca
      541 gtatagaaga cataaaaaaa cataaattcg tacccttgta tgtagacgcg aatacgatgc
      601 gttttggtat taccaatacg acacctcaag ctattttgca aaaactgcgc caagatcatc
      661 tagaccaaag aatagagttt tacttaattt ctgacagtgg ctatcgtgat ttattacgaa
      721 aatacgaccc acccaaacaa gttatttacg atgatattaa tattggtaat actacaaata
      781 ctgagcaagt agctacaatt tcccatacac tcggctcggt gcgggcagat gatatgctcg
      841 catacgttgt aaagcaagca tatcagcttc acgcgtctga tatacatcta gagacagatc
      901 gaaatgaagt tcgtatccgg tttagggttg atggcgtgtt gcacccaatt gccttgctca
      961 gtatagaaaa atatcggcaa ttagtatcaa gtctcgctgt agcagcaaac atatctacca
     1021 gttcggcaga tgctcaaact ggacacataa ataagaatta tatgcttgcc gatggttcta
     1081 atgtagaagt aaacctgcgt gtagaaacgg taccgactgt acatggtatg gacgcagttc
     1141 tgcggttgtt cacgttaaat acagacctta tgcaattgca taaattgaac cttcaaccag
     1201 acgagctaca tatagtcaaa gaagtaatta gccatcctaa tggccttgta ctgattgttg
     1261 gtccaaccgg ttcaggtaaa actactacgt tgtatagcat tctaaatgaa ctcaatagta
     1321 ctgaacggaa attaataaca ctcgaagacc cagttgaata caatatcaaa ggggtaaccc
     1381 agataccggt tgattctcgg caggttaaaa caggttttgc tgaaaagttc cgggcggtgc
     1441 ta
//
LOCUS       DAOPLS010000208         1438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_107404_length_1438_cov_4.608821, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000208 DAOPLS010000000
VERSION     DAOPLS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107404_length_1438_cov_4.608821"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKV52_04125"
     CDS             <1..384
                     /locus_tag="PKV52_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPD99430.1"
                     /translation="ALKLKEISYIHAEGYAAGEMKHGPLALIDTSFPTFALAASPTLF
                     EKTASNLQEVKARNGKIIAVTTKKHVPQLKDLADDIINIPEASDQLLPLLEAIVVQLY
                     AYYTALELGHDIDKPRNLAKSVTVE"
     gene            complement(438..764)
                     /locus_tag="PKV52_04130"
     CDS             complement(438..764)
                     /locus_tag="PKV52_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99431.1"
                     /translation="MTDYKVGRRKWASFTIFEQMGNIYSEVGRSFSAQKLNDTKAKDA
                     AMVRAIDLFDATTEELVKNKSPKTKEVLRAKEMYLKTLFDGDEIQRSEIEKYFMQFAI
                     AARLHK"
     gene            complement(757..1101)
                     /locus_tag="PKV52_04135"
     CDS             complement(757..1101)
                     /locus_tag="PKV52_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HPD99432.1"
                     /translation="MKTIDTISVTELTEMAENMYGNLVKAVVDINKNILVVDAEMHAD
                     EEQFLLENGSDQADLWGINLYPAKFGSEDFIEFDSMINIRPRQNNMTRGVGDPDLREK
                     IQTIVTKKVKND"
     gene            complement(1175..>1438)
                     /locus_tag="PKV52_04140"
     CDS             complement(1175..>1438)
                     /locus_tag="PKV52_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99433.1"
                     /translation="PNFEGSDGLSSFMESLLQGTSMSELESKPDVTIDGVTAKVYGFN
                     GLSYIEWYVFENNGIYYGVEVAGADQEPSKSIIKEVIAGFSFN"
BASE COUNT          448 a          327 c          217 g          446 t
ORIGIN      
        1 gcgcttaagc tcaaagaaat tagctatatt cacgcagaag gctacgctgc aggcgaaatg
       61 aaacacggcc cactagcgct catagacacc agctttccta cctttgcgct tgctgctagc
      121 cctacactat ttgaaaaaac agccagcaac ttgcaagagg taaaagcccg caacggtaaa
      181 attatcgccg ttactaccaa aaaacacgta ccacagctaa aagaccttgc agacgatatc
      241 attaacatac cagaagcttc agatcagtta ttaccacttc tagaggctat tgttgtgcaa
      301 ctatatgcct actacaccgc tctagaactc ggccacgata tagacaagcc ccgcaactta
      361 gccaaatcag tcaccgtaga ataacaaatc atacgagcag ataatataaa gcttagttgt
      421 ttggttgaat acgataatta cttgtgtaaa cgtgcagcaa tagcaaattg cataaagtat
      481 ttttcaatct cacttcgctg tatttcatca ccgtcaaaaa gtgtcttcaa gtacatttct
      541 tttgcccgta atacttcttt agtttttggt gacttattct tcacaagttc ttctgtagtc
      601 gcatcaaata agtcaattgc ccgcaccata gcagcatctt tagcttttgt atcattaagt
      661 ttttgtgcac taaaagatcg gccaacttcg ctataaatat tgcccatttg ttcaaaaatc
      721 gtaaaagatg cccatttacg cctaccaacc ttgtaatcag tcatttttaa cctttttggt
      781 aacaatagtc tgtatctttt cacgtaaatc tggatcacct actccacgtg tcatattatt
      841 ttgccgggga cgaatattta tcatagaatc aaattcaata aaatcttcac taccaaactt
      901 ggctggatac aaatttatac cccacaaatc tgcttggtct gatccatttt ctaacagaaa
      961 ttgttcttcg tctgcgtgca tttcagcatc aactaccaaa atgtttttat ttatatctac
     1021 tacagctttc actaaattac catacatgtt ttctgccatt tctgttagtt cagtaacaga
     1081 tattgtatct atagtcttca tcgactaaat tatagcacct caaatatata gatgctgtaa
     1141 ttatggggtc aagttgtggt cttcgtatcg tagattaatt aaaagaaaaa ccagcaataa
     1201 cttctttaat gatagattta ctaggttctt ggtcagcccc agcaacttca acaccgtaat
     1261 aaataccgtt attttcaaaa acgtaccatt caatataact aagtccatta aagccgtata
     1321 ctttggcggt aacaccatct atggttacat ctggtttgct ttctagctca ctcatgctcg
     1381 taccttgtag caaagattcc ataaaactac tcaaaccatc cgagccttca aagttagg
//
LOCUS       DAOPLS010000209         1437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_107554_length_1437_cov_2.979740, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000209 DAOPLS010000000
VERSION     DAOPLS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107554_length_1437_cov_2.979740"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKV52_04145"
     CDS             <1..648
                     /locus_tag="PKV52_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99434.1"
                     /translation="ASAADKTALAAGTAIIAVILTLLTIFVSIVLYPALIITQLESVK
                     GNKISLSEAFNGGLNYVLRFIIAGLIGFVIVGGPIIISLLLMFVLIGFVLLPFALLWA
                     VVATFFLFLTPYIIITQNLDAVSAIKKSYETTRRKWQWVLALYTITALLSIVSSVPVI
                     GGLAGLVGTIIFFCLPAIIYLRHITPAEKAPASSTQKSKASPKKPKRKATTKKSA"
     gene            complement(709..>1437)
                     /gene="tyrS"
                     /locus_tag="PKV52_04150"
     CDS             complement(709..>1437)
                     /gene="tyrS"
                     /locus_tag="PKV52_04150"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPD99435.1"
                     /translation="DFLHLSREKGVTLQVAGSDQWGNSIAGVDLVRRIDNQEVHVYTA
                     PLVINKTTGKKFGKSEDGAVWLDPDMTSVYQFYQFWLNVDDEGVEDYLKIYTLLSKEE
                     TERILSDFNSNRAGRLAQKTLAYEVTKIVHGQERADSVKRISEVLFGGRSYNELTQAD
                     FEALGKEIGIIATKDGTELAEILVQARLTKSKSEARRFITSGAIYVNGIQVAADDLVL
                     RADQSINGHYILRRGKNSQIVLAL"
BASE COUNT          387 a          332 c          267 g          451 t
ORIGIN      
        1 gcaagtgcag ctgataaaac cgcccttgca gctggaactg ctattatagc cgttatactg
       61 acactcctca cgatttttgt gtctattgtc ttgtacccag cattaataat tacgcaacta
      121 gaaagcgtta aaggtaataa aatttcttta agcgaggcgt ttaatggagg gttaaactac
      181 gttttacggt ttattattgc tggactgatt ggatttgtta ttgtgggcgg accaattatt
      241 atctctttac tactcatgtt cgtactcatc ggttttgtgc tactaccatt tgcgttgcta
      301 tgggcggtgg tcgcaacgtt ctttctcttt ttgacaccct atattatcat tacccagaac
      361 ctagatgccg taagcgctat taagaaaagc tatgaaacaa ccaggcgtaa atggcagtgg
      421 gtgctcgctc tttataccat cactgcgcta ctatctatcg tatcttcagt accagttatc
      481 ggaggacttg ccggcttagt agggacgata atattctttt gcttacctgc aattatatac
      541 ttacgccaca ttacacctgc cgaaaaagcg ccagcaagct ctacgcaaaa aagtaaggcg
      601 agcccaaaaa agccaaaacg taaagctact acgaaaaaat ctgcataaat aacgtagcca
      661 ccacaaaaca ccgtcttacc agcatttttg cagagacggt gttttatttt atagcgctaa
      721 aactatctgt gagtttttgc cacggcgcaa aatatagtgt ccattgatac tttggtcagc
      781 tctaagtact aaatcatcag cagctacctg tataccatta acgtatatag caccactcgt
      841 aataaatcgc cgcgcttctg atttactttt tgttaagcgc gcctgcacta atatttctgc
      901 tagttcggtg ccatctttag tagcaataat gcctatctct ttaccaagtg cttcaaaatc
      961 tgcttgtgtg agttcgttgt aactacggcc tccaaacagc acttcactaa tgcgctttac
     1021 actatctgcc cgctcttggc catgcacaat ttttgtaact tcgtatgcta aagttttttg
     1081 ggctaagcgc cctgcacggt tactattaaa gtccgataaa atacgctctg tttcttcttt
     1141 gctgagcagt gtataaattt ttaggtaatc ttctacacct tcatcatcta cgtttaacca
     1201 aaattggtaa aactgataaa cactagtcat gtctggatct agccaaacag cgccgtcttc
     1261 gcttttgcca aactttttac cagtggtttt gtttatgaca agcggcgcag tatacacgtg
     1321 cacttcttgg ttatctatgc gtcgtacgag gtctacacca gcgatactgt tgccccactg
     1381 gtctgaccca gcaacctgta aagtaacgcc tttttctctt gataaatgca agaaatc
//
LOCUS       DAOPLS010000210         1435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_107764_length_1435_cov_3.270290, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000210 DAOPLS010000000
VERSION     DAOPLS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107764_length_1435_cov_3.270290"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKV52_04155"
     CDS             <1..610
                     /locus_tag="PKV52_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99436.1"
                     /translation="QTYQTLAKEYSTKQLLEAGILTERANGLTPLFYKHTVAVPFYDM
                     NGLIYLRARTLDPTVKAKYLAPRGTSPPIYNYQTIAEYDGSRPLYITESETDALALNV
                     IGETALALVGGQKHIDSLVVRELAHLITYGLSPKIEVNIVADRDTTGEAFFKSVADAL
                     YIAGVPANNLNKYQSDEQYKDIAEQVKAIKTTERANGYKGKK"
     gene            607..936
                     /locus_tag="PKV52_04160"
     CDS             607..936
                     /locus_tag="PKV52_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99437.1"
                     /translation="MSKKKDLEEFEVLMDDLRQMVYDLDERDRKRAEKEQNKLLSELP
                     EWREYQRESRKNGKRAKHNKEFFKKLEANKAKANYKKVYQIERSTNSLKPKPESLLDK
                     FKNWLRS"
     gene            938..>1435
                     /locus_tag="PKV52_04165"
     CDS             938..>1435
                     /locus_tag="PKV52_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPD99438.1"
                     /translation="MQPTIKYQPDDKAKLEKATRLLPLTNELEQYRQELGDGSKTFRG
                     LVTGFNEVDDLISGLDRFILLAGRSGAGKTTLATQLALGVATHTPVLFYSLEMSKGEI
                     TTMMLQVLAKGLYRNDIELRGNDPELQADKQTKLDTAFNKLAELAGNVYIKDATTGIP
                     RIADPN"
BASE COUNT          543 a          306 c          321 g          265 t
ORIGIN      
        1 gcagacctac caaacactag ctaaagagta cagcaccaaa cagctactag aagcgggtat
       61 attgaccgaa cgggcgaacg gtttaacacc tttattttat aagcatacag tagctgtacc
      121 tttttacgat atgaacgggc taatatacct acgggctaga accttagacc cgacagtaaa
      181 ggctaaatac ctagcaccta gaggcactag ccccccgata tacaactatc aaacaatagc
      241 cgagtatgac ggcagtagac cgctatatat taccgagagc gaaacagacg ctttagccct
      301 aaacgtaata ggcgaaaccg ccctagcact tgtaggcggg caaaagcata tagacagctt
      361 agtagtacga gagttagccc acctaatcac ttacgggcta agccctaaga tagaggtaaa
      421 catagtagcc gaccgagata ctacgggcga ggcatttttt aagagcgtag cagacgcttt
      481 atacatagca ggcgtaccag ctaacaacct gaacaagtac cagagcgacg aacaatataa
      541 agacatagcc gagcaagtaa aggctattaa aacaaccgaa cgggcgaacg gttataaagg
      601 taaaaaatga gcaagaaaaa agatttagaa gaatttgaag tcttaatgga cgacttaaga
      661 cagatggttt atgacttaga tgagagagac agaaaaaggg cagaaaaaga acaaaataag
      721 ctactatcgg aactaccaga gtggagagag taccagagag agagtagaaa aaacggtaaa
      781 agggcaaagc acaacaaaga attttttaaa aaactagaag caaataaagc taaggcaaac
      841 tataaaaagg tataccaaat agagcggtct actaactcac ttaaaccaaa gcccgagagc
      901 ttgctagata aatttaaaaa ctggctaagg agttaatatg caaccgacaa taaagtacca
      961 accagatgac aaagcaaaat tagaaaaggc tacaaggcta ttaccgctta ctaatgagct
     1021 tgagcagtac cgccaagaac taggcgacgg cagtaaaacc tttaggggct tagtaacggg
     1081 ctttaacgaa gtagacgacc taataagcgg gctagacagg tttatattac tggcaggcag
     1141 aagcggggcg ggcaagacaa cactagcaac ccagctagca ctaggcgtag caactcatac
     1201 ccccgtactg ttttatagcc ttgaaatgag caagggcgag attactacaa tgatgttaca
     1261 ggtactagct aaagggcttt accgtaacga tatagagcta cgaggcaacg acccagagct
     1321 acaagccgat aagcaaacca agctagatac cgcctttaac aaactggcag agctagcggg
     1381 caacgtatac ataaaagacg ctacaacagg cataccccgc atagcagacc ctaac
//
LOCUS       DAOPLS010000211         1433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_107973_length_1433_cov_3.674891, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000211 DAOPLS010000000
VERSION     DAOPLS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107973_length_1433_cov_3.674891"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKV52_04170"
     CDS             complement(<1..340)
                     /locus_tag="PKV52_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPD99439.1"
                     /translation="MIQAVIFDMDGLLIDSEPMWKESEVKIFTQYGVPLTYAMTKQTM
                     GLRTEDVVNYWYHRYPWAATPKNVVVRQIDQLVLDIIQEKAKPMMGVIDVLNNCKQAG
                     LQMAVASSSSL"
     gene            443..>1433
                     /gene="lexA"
                     /locus_tag="PKV52_04175"
     CDS             443..>1433
                     /gene="lexA"
                     /locus_tag="PKV52_04175"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPD99440.1"
                     /translation="MSTKTDDILAFIKAYHKQEGYSPSYKEIGAKFRIASPSVIHYHL
                     NKLQQQGKISKGPYSTRSIFPETETQYIHVPLLGEISAGKPIRAVSQQELFTIPASSI
                     KAKDSKNLFALRVKGSSMVDDNIHDNDIVIVRQNSTPKNGERVVALIDNDYVTLKRFY
                     QEPGRVRLQPSNNKYKPIYVLPSQSLSIQGTVASVIKAPHIKLKQLNKKTAVAHTPSS
                     IEKNKIIVGDAVQEMTKLPDASCDVVLIDPPYNIGKDFGNNSDNRELADYIAWCKEWL
                     FQAERVLKPHGTMFIYGFSEILAHISVELTLPKRWLVWHYTNKNVASLHFWQRS"
BASE COUNT          464 a          305 c          258 g          406 t
ORIGIN      
        1 ctaaagatga agaagaagca acagccatct gtaaaccggc ttgtttgcaa ttattaagta
       61 cgtcaattac acccatcatt ggcttcgctt tctcctgaat aatatctaaa acaagttgat
      121 caatttgtct tactacaacg tttttcggcg tagcagccca agggtaccga tgataccaat
      181 aatttactac atcttctgtt ctcaaaccca tcgtttgctt ggtcatcgcg tacgttaatg
      241 gtaccccata ttgagtaaat atttttactt cagattcttt ccacataggc tcactgtcta
      301 taagcaaacc gtccatatcg aatattactg cctgtatcat gatgatatta tagcctattg
      361 cttatacagc ctgctttgtt atcgtatatt agaccgtacg cacgtgttgt tttctaattt
      421 ttttctatat ataatgtctg taatgagtac aaaaacagat gatatcttag cgtttattaa
      481 agcctatcat aaacaagaag gctacagccc atcatacaaa gaaattggag ctaaatttcg
      541 catagcttca ccatctgtta ttcattacca tctaaacaaa ctccagcaac aaggaaagat
      601 atctaaaggt ccatattcta cccgcagtat ttttccagaa acagaaacgc aatatattca
      661 tgttccgtta ctaggtgaga tttctgctgg taagccgatt cgtgctgtaa gccaacaaga
      721 attattcacc atacccgcgt ctagtataaa agcaaaagac agcaaaaacc tatttgcgct
      781 ccgtgtcaaa ggtagcagta tggttgacga taatattcat gataatgaca ttgtgattgt
      841 acgacaaaac tcaacaccaa aaaatggtga aagagtcgtt gctttgatag ataacgatta
      901 cgtaacacta aagcgtttct accaggagcc gggtcgtgtt cgtttgcagc cttctaataa
      961 taagtataaa ccaatctatg tattgccaag tcaaagccta agcatacaag gaacggtcgc
     1021 ttctgtgatc aaagcacccc acataaagct caagcaactt aataaaaaaa ccgcagtagc
     1081 acatacgcct agtagtatag aaaaaaataa aattatcgtc ggggatgccg tgcaagaaat
     1141 gactaaacta ccagatgctt cgtgtgacgt cgtccttata gatccgccat acaacatagg
     1201 caaagacttt ggcaataata gcgacaaccg cgaattagct gattatatag cgtggtgcaa
     1261 agagtggctt ttccaagcag agcgcgtact gaagccacac ggtacgatgt ttatatatgg
     1321 attcagcgaa atactcgcac atatttctgt agagctcaca ttacctaagc ggtggttggt
     1381 atggcactac acaaataaga atgtcgcctc actacatttt tggcaacgca gtc
//
LOCUS       DAOPLS010000212         1431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_108289_length_1431_cov_1.664971, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000212 DAOPLS010000000
VERSION     DAOPLS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108289_length_1431_cov_1.664971"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKV52_04180"
     CDS             complement(<1..414)
                     /locus_tag="PKV52_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPD99441.1"
                     /translation="MEYSLASSTWGPEEYAAIDRVVKTGMFTMGEHVAAFEQEFADKF
                     GCKYGVMSNSGSSANLLAIAALFYKKDNPLKPGDEVIVPAISWSTTYYPLLQYGLKLR
                     FVDVELDTLNMDVTKFEEALTSKTRLVVAVNILGNP"
     gene            complement(447..>1431)
                     /locus_tag="PKV52_04185"
     CDS             complement(447..>1431)
                     /locus_tag="PKV52_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPD99442.1"
                     /translation="SERVIGYVDALREALHYKMENDKSVFMLGQGVDDPGGMFGVTRN
                     FHEVYGRTRSFDTPVSEEGVMGICTGAAMNGMRPVYFHNRPDFLLLTFNQIVNHASKM
                     HWMDNGTTNVPMTVWAAIGRGWGSGPQHSQAIQGLMLSVPGIKIIMPSTPYDAKGLMI
                     SAIEDNNPTFIFEHRWLTRREGSVPEEAYRVPIGKGIHRREGKALTIVGTSHAIDLAW
                     TAAKELAADSGIDAEVIDLRTLKPLDEEIILESVAKTGKLLVVDTGWAMGGVCAEIGC
                     LVAEKAFNHLKAPVMRVGLPDCPSPAGYTLEQLYYPDVNTMKKAIIELHNK"
BASE COUNT          443 a          353 c          240 g          395 t
ORIGIN      
        1 tggattacca agaatattta cagcgacaac aagacgtgtt ttagatgtca atgcttcttc
       61 aaacttggtc acatccatat ttagtgtatc aagctccaca tcaacaaaac gcaatttcaa
      121 accatactgt agtaatggat agtaagtagt tgaccatgaa attgctggca cgatcacttc
      181 atcacccggc ttaagcggat tatctttttt ataaaaaagc gcggcaattg caagcaaatt
      241 agcagaagaa cctgaattac tcatcacgcc atatttacag ccaaatttat cagcaaactc
      301 ttgttcgaat gcggcgacat gctcgcccat ggtaaacatc cctgttttaa ccacacgatc
      361 aatagcagca tactcttcag gaccccaagt actacttgcc aacgaatatt ctatatttgc
      421 aacagtttca gttgtcataa ttaaccttat ttattgtgaa gttcaataat tgcttttttc
      481 attgtattaa catccggata atacaattgt tctaaagtat aacctgcagg tgaagggcag
      541 tcaggtagac caacacgcat tacgggtgct tttaaatgat taaaagcttt ttctgcaact
      601 agacaaccaa tttcagcaca aacgccgccc atcgcccatc ctgtatctac aaccagaagc
      661 ttccctgttt ttgcaactga ttctaaaatg atttcttcat ctagcggttt tagtgttcgc
      721 aaatcaatca cctctgcgtc gatacctgaa tctgcagcta attctttagc agcagtccaa
      781 gctaaatcta tcgcatgaga agtaccaaca atagttaatg ccttaccctc acgacgatgg
      841 atgcccttgc caataggcac gcgataagct tcttcaggca cactaccttc tcgtcgcgtt
      901 aaccaacgat gctcaaaaat aaatgtggga ttattatctt cgatggctga aatcatcagt
      961 cctttagcat catagggcgt acttggcatg ataattttaa taccaggaac actcaacatc
     1021 agaccctgga tcgcttgtga atgctgagga cctgagcccc aaccacgccc aatagcggcc
     1081 cagacggtca ttggtacgtt cgttgtacca ttatccatcc aatgcatctt actcgcatga
     1141 ttgacaattt ggttgaatgt taataacaaa aaatcaggtc gattatgaaa gtaaactgga
     1201 cgcatgccat tcatagccgc acccgtacaa atacccatta cgccctcttc tgaaaccggc
     1261 gtatcaaatg aacgcgtacg accatatact tcatgaaagt tacgtgtgac accaaacatt
     1321 ccaccaggat catctacacc ctgacccaac ataaaaacac ttttgtcatt ttccatttta
     1381 taatgcaaag cctcacgcaa ggcatccaca taaccaatga cacgctcaga t
//
LOCUS       DAOPLS010000213         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_109740_length_1419_cov_2.131965, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000213 DAOPLS010000000
VERSION     DAOPLS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109740_length_1419_cov_2.131965"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PKV52_04190"
     CDS             <1..918
                     /locus_tag="PKV52_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99443.1"
                     /translation="VWATFDAIGDSSHGKPVVFTYKNKSYNYPTRSEAEAMIVIQCIV
                     AGYDLEKVYSLFKQQQLDLNKGGSFAEETNKWQYIKQLYESGLRVYHNDPIRQEMQQL
                     SAYSVPLNHTSKQQEATVRTVLNGLLEIAYRYRTDKPRASYRQLQMLGNIGSTRTVGN
                     ALAKLVEEGLITQLKSQSEWTANVYVLNMPMLLKHTHNQHTESVSLLHYLLNTKETMI
                     YMAMVDTPLTTAGIAKKVARQPRTVNKVLAELKTRGFIDEQDDKYCVMPLNMVQFTSE
                     IDYFLGERMTKIATQRKLHDLRMEAHVNG"
     gene            911..1219
                     /locus_tag="PKV52_04195"
     CDS             911..1219
                     /locus_tag="PKV52_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99444.1"
                     /translation="MADILLCPVCNDSYNHIYRHEIYPVLGTRKYTIACGVFDVNYDK
                     KAFGVRGFTFETYYQCEGGHKWMQTHQFHKGEVFVETEVIDARDSYDEDDFMDDIWRD
                     "
     gene            1231..1392
                     /locus_tag="PKV52_04200"
     CDS             1231..1392
                     /locus_tag="PKV52_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99445.1"
                     /translation="MCEIIDKHLVPVEWNDDYIVFLLNDVTPITIEWRALNPFMWATL
                     VDYLLVILD"
BASE COUNT          475 a          241 c          286 g          417 t
ORIGIN      
        1 gtttgggcaa catttgatgc tattggtgat agttcacatg gtaagcctgt tgtatttaca
       61 tacaaaaata aatcatataa ctacccaaca cgatccgaag ctgaagcaat gattgttatt
      121 caatgcattg tggccggtta tgacttagaa aaagtttata gtttattcaa acaacaacag
      181 cttgatttga ataaaggggg ttcatttgcc gaagaaacaa acaagtggca atacatcaaa
      241 caattatatg aaagtggttt acgggtgtat cacaatgatc ctatccggca agaaatgcag
      301 cagttatctg cttatagtgt accattaaat catacaagta aacaacaaga agcgactgtt
      361 agaacagtgt taaacggttt acttgaaatt gcctatcgtt atcgtacaga taaaccacgt
      421 gcttcatatc gccaactaca aatgttaggt aatattggtt ccacgagaac agtcggcaat
      481 gctttggcta aattagttga agaagggctg attacacaac ttaaaagtca atctgaatgg
      541 actgccaatg tttatgtgct aaacatgcca atgttgctaa agcacacaca caaccaacat
      601 acggagtctg tttccctttt gcattatttg ctaaacacaa aggaaacaat gatttacatg
      661 gcaatggttg ataccccact gaccacagca gggattgcca agaaagtagc cagacaacca
      721 cgtactgtaa acaaagtatt agccgaactg aaaacacggg gtttcattga tgaacaagac
      781 gataaatatt gtgttatgcc gctaaatatg gtacaattta cctcagaaat tgattacttt
      841 ttaggtgaaa gaatgacaaa aattgcaaca caacgtaaat tacatgattt gagaatggag
      901 gcacacgtaa atggctgata tattgctatg ccctgtttgc aatgattcct ataaccatat
      961 ctatcggcat gaaatatacc ctgtgttagg tacaagaaaa tacactatcg catgtggtgt
     1021 tttcgatgtt aactatgaca aaaaagcctt tggtgtacgt ggttttacgt ttgaaacgta
     1081 ctatcaatgt gaaggtgggc ataaatggat gcaaacacac cagtttcata aaggcgaagt
     1141 ctttgttgaa actgaggtca ttgatgcccg tgatagttac gatgaagatg atttcatgga
     1201 cgatatttgg cgggactaat gtttgaaaca atgtgtgaaa tcatcgacaa acatttagta
     1261 cctgttgaat ggaatgatga ctacattgtg tttttgttaa atgatgttac cccgattaca
     1321 attgaatggc gtgcgctaaa cccgtttatg tgggcgacac tagttgatta tcttttggta
     1381 atacttgact aattttacaa atttttgtaa actggtgat
//
LOCUS       DAOPLS010000214         1418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_109805_length_1418_cov_3.258254, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000214 DAOPLS010000000
VERSION     DAOPLS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109805_length_1418_cov_3.258254"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1418
                     /locus_tag="PKV52_04205"
     CDS             <1..>1418
                     /locus_tag="PKV52_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HPD99446.1"
                     /translation="KDGSTAFGKKILIDGQQRVTAMQASIVGQKIVNKEYKEVRVRIS
                     FNPLTEDFRTRTAVTSKEPEWIEDISEIMKDDASLFEIVSAYMARNPAADQKKIEQNI
                     QKLINVKTKQIGMIELDASLDIETVTEIFIRINSQGVVLSQADFAMSKIAAYGEFGVN
                     LRKTIDYFCHFAIAPHFFKMVAENDQEYAKTDYIHKIEWLKNENDDLFDPDYNDLMRT
                     ITIHEFKRGRVADLVSLLSGRDFETREYKPEIAEDSFNRLEKAILQYVNETNFKRFLM
                     ILKSAGYISKDMITAKNALNFAYALFLKLRDDGERPEVIESVVRRWFVMSMLTGRHSG
                     SFESVFESDIRKIDEQGAKQYLASIEEGELSDAFWSVRLVQELEKSSIRNPYINAFFA
                     AQVYFEDKGFLSKDIKVRDMIEHAGDIHHIFPKRYMIKNGYDRWTYNQVANYVQAQTE
                     INIAISSKEPKQYMHDVLAQCN"
BASE COUNT          518 a          233 c          275 g          392 t
ORIGIN      
        1 taaagatggt tcaactgcgt ttggtaaaaa aatacttata gatggccaac agcgcgtcac
       61 ggcaatgcaa gcttctatcg ttggccaaaa aatagtaaat aaagaatata aagaagtccg
      121 tgtaaggatt tcattcaacc ctttaactga ggattttcgt acaagaacag ctgttacatc
      181 taaagagcct gaatggattg aagacatttc agaaattatg aaggatgatg caagtctttt
      241 tgaaatagta agtgcgtaca tggcccgtaa tccagcagcc gaccaaaaga aaatagaaca
      301 aaacattcaa aaacttatta atgtaaaaac aaaacagatt ggtatgatag agctcgatgc
      361 cagcttagat atagaaacgg taacagaaat attcattcgt ataaattcac aaggtgttgt
      421 gttaagtcag gcagattttg caatgagcaa aattgctgct tatggtgaat ttggtgttaa
      481 tctacgtaaa actatcgatt atttctgcca ttttgctata gcaccacact tctttaagat
      541 ggttgcagaa aacgaccaag aatacgcaaa gacagactat attcataaaa tagagtggct
      601 aaagaatgaa aacgatgatc tatttgaccc agattacaac gaccttatgc gaaccatcac
      661 aattcatgaa tttaaacgtg gtagggttgc agacttagtt tctttactat ctggtagaga
      721 tttcgaaact cgtgaataca agccagaaat cgcagaagac tcatttaaca gattagaaaa
      781 agcaattttg caatatgtaa acgaaactaa ctttaaacgt ttcttaatga tattaaaaag
      841 tgcaggttat atatccaaag acatgataac cgctaaaaac gcacttaatt ttgcatatgc
      901 ccttttctta aagttgcgtg atgacggcga gcgcccagaa gtaatagaat cagttgttag
      961 gcgttggttt gttatgtcta tgttaactgg tagacactct ggttcattcg aatcggtttt
     1021 tgaatctgat attagaaaaa tagatgaaca gggcgctaaa caatacttag cctcaataga
     1081 ggaaggcgaa ttaagtgatg cattttggag tgtgcgttta gtacaagaac tagaaaaatc
     1141 tagtatacgt aatccataca taaatgcatt ttttgcagcg caggtatatt tcgaagacaa
     1201 aggcttttta tcaaaagata taaaagtacg cgatatgata gagcacgccg gtgatattca
     1261 tcatatattc cctaaacgct acatgataaa gaatggctat gatagatgga cctacaatca
     1321 agtagctaat tatgtgcaag cgcaaacaga aattaatata gccataagca gcaaagaacc
     1381 taagcaatat atgcacgatg tactcgcaca atgcaatg
//
LOCUS       DAOPLS010000215         1412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_110579_length_1412_cov_1.375092, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000215 DAOPLS010000000
VERSION     DAOPLS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110579_length_1412_cov_1.375092"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..262
                     /locus_tag="PKV52_04210"
     CDS             86..262
                     /locus_tag="PKV52_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99447.1"
                     /translation="MIISVKEARKILGKDYEKLTDEEVEKLIYQLHNLAKASLEMARK
                     QRLEKTLGETTPEN"
     gene            343..1242
                     /locus_tag="PKV52_04215"
     CDS             343..1242
                     /locus_tag="PKV52_04215"
                     /inference="COORDINATES: protein motif:HMM:NF037888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99448.1"
                     /translation="MTDTELFQQKFTQWREYLGSDNNADINTIRLTLSAIFNDMGAYD
                     AFEAVCKASPSTPISTMLFGNLTSLSYFQLQIMRIRRLIDKPAVRKNKDVSIYSLRRV
                     IDEIKEERDAGRFTRGNVCNAFNIAPSKTEVEAQFVADISNANGGSFSTGSIIAGKIH
                     TMLDGIFDTNGSLKQEIVDELEKRVYRDKNDKLKSITDVADKYIAHSATPDSRTSDNA
                     NNLVIAMSDLKEVANGLLEAFYICTWIIEYQVANPVHTSVGNSIVNLTSDQQTLANET
                     YETLRKNVDTLIDNAKGIVGITD"
BASE COUNT          445 a          268 c          314 g          385 t
ORIGIN      
        1 tattgaacaa gcgatctgac ggcttatacc tgtttgctgg tgctgaatat acacctgacc
       61 ttgaagcgtg gactaggttt tcggtgtgat catatccgtt aaagaagcac gcaagatact
      121 tggcaaagat tacgaaaagc tgaccgatga agaagttgaa aaactaatct atcagctaca
      181 taatcttgcc aaagcttcgc ttgaaatggc acgcaaacaa cgccttgaaa aaacgcttgg
      241 cgaaactacc cccgaaaatt aggcatttat acagcggagt gctgaaaagc taaaacaagg
      301 ggcgactacc cccgtatgtc agtttgattg tataatatgc ctatgactga tacggagtta
      361 ttccaacaga agtttaccca atggcgtgaa tatcttgggt ctgacaacaa tgctgatata
      421 aacacgatca ggctgacgct atcagccatt ttcaacgata tgggtgcgta tgacgctttt
      481 gaagctgttt gtaaggcaag tccgtctacg cctatatcaa caatgctttt tggtaatcta
      541 acttctttga gctacttcca attacagata atgcgtatac ggcgtttgat agacaaacct
      601 gcggttcgca aaaataaaga tgtcagcatt tattctttgc gtagggttat agacgaaatc
      661 aaagaagaac gtgacgctgg cagatttact aggggaaatg tttgtaacgc attcaacata
      721 gcaccatcta aaaccgaagt tgaagcacag tttgtggctg acatatctaa tgccaacggt
      781 ggtagttttt cgactggttc aataatagct ggcaaaatac atactatgct tgacggcata
      841 ttcgatacga atgggtcgct gaaacaagaa atcgtagatg agcttgaaaa gcgtgtttat
      901 agggataaga acgataaact gaaaagtatt acagatgtag cagataaata catagcacat
      961 tcggcgacac ccgacagcag aaccagtgat aacgccaata atttagtaat agctatgtcc
     1021 gacctgaaag aagtagcaaa cggcttgctg gaagcttttt acatctgcac ttggattatt
     1081 gaatatcagg tggctaatcc tgttcataca agcgtgggta attctatcgt caatctgaca
     1141 agtgatcagc aaactttggc gaatgaaaca tacgaaacat tgcgtaaaaa cgtagacacg
     1201 ctaatcgaca atgcgaaagg catagttggc attacagatt gacttggctt tctacgctag
     1261 ttttcttttt ggatcttgct gttcaagact aatcatctgt ggattgacta cgaaattgtg
     1321 cgagtagaag aaatcggtta gctcggtcgg tatctgcttt tttgaagcgt aattaggaaa
     1381 tatatgtggc ggttcaagtg acccgtcata ct
//
LOCUS       DAOPLS010000216         1405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_111392_length_1405_cov_2.531852, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000216 DAOPLS010000000
VERSION     DAOPLS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111392_length_1405_cov_2.531852"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..838)
                     /locus_tag="PKV52_04220"
     CDS             complement(341..838)
                     /locus_tag="PKV52_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99449.1"
                     /translation="MTSLYTNKNVQITKIGIISVFIGLLIFMLITISSSVHASGGAFT
                     QPTHDGPIKPRPVISQQQGYFSLATTTEHMTFGVDTSIYSLPINVSVKLPYEHQVASK
                     ATGLPTDQIPAEKPFTAVDYAAAVDTKHSDPAVASTFAYLLIGALLWYVVTFYLPKQN
                     QPHVT"
     gene            967..>1405
                     /locus_tag="PKV52_04225"
     CDS             967..>1405
                     /locus_tag="PKV52_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPD99450.1"
                     /translation="MFEKLTVTLPIHAVLQTYKRAYIRRDVIAGATVAAVAVPQAMAY
                     AQLAGVNLTAGLYAAMVAMVVYAIMSSSRYVIMGPDAAMAALAGAAVIPLSGGDANRA
                     VALVAILSFFIGLVSIISVIARIGFVAEFLSRPILLGYMAGLAL"
BASE COUNT          347 a          282 c          341 g          435 t
ORIGIN      
        1 ctttaggcat tagctcattc atccctccct tttgtgagcg ggcgcctaac cccggtctca
       61 gtacgcacct ttttatgggt gcttgtaggg tgaagtcgtt gttacttaat tgacgccagc
      121 cgcgtcactt cacccttgct ggtctacccc tcgctattac gaggggttac tttaggagaa
      181 ccgtttgtac actacacgtt cgtagtgggc gtccagcagc gccttagtat cccctgctgg
      241 ttgtactact tattttacct ttaaggctgc tgcctcttgg gtatttataa taatagcaca
      301 aaattagtat aaagtcaaca aatatgtacg gggtatgctg ctaggtaacg tggggttggt
      361 tttgctttgg gaggtagaat gttactacat accacagtag cgcaccaata agcaggtacg
      421 caaatgtaga cgctacagct gggtcgctat gtttggtatc tactgcagcg gcgtaatcta
      481 cagcagtaaa tggtttttct gctggtattt gatctgtagg taagcctgta gctttactcg
      541 caacctgatg ttcgtatggc aatttaacag aaacattaat gggtagcgag taaatgcttg
      601 tatccacgcc aaatgtcata tgttctgtgg tggtggcaag gctaaaataa ccctgttgct
      661 ggctaataac ggggcgtggt ttgataggcc cgtcatgtgt gggttgtgtg aatgcccctc
      721 ccgatgcgtg tacagagctg ctgatagtaa tgagcataaa aattaacaga ccaataaata
      781 cactaataat tccaatcttg gtgatttgta cgttcttatt ggtatataac gatgtcatga
      841 tacgttagcc actatgtgaa ttttcgttta attacttgat agttgttatt atgcgctctt
      901 tagcttattc gtcaatattt actgatacag atgaaatagc ttggtaattg gcgtacaata
      961 aagataatgt tcgaaaaact aacagtcacc ctaccaatac atgctgtatt acaaacatat
     1021 aaacgtgcgt atatacggcg tgatgtaatt gctggagcaa cagtagcagc agtggccgtg
     1081 ccacaagcca tggcgtatgc acagttagct ggtgttaatt taacagctgg tttatacgca
     1141 gccatggtag caatggtggt gtatgcaatt atgagttcat cgcgctacgt aattatgggt
     1201 ccagatgcgg ccatggcagc actcgctggg gcagccgtta taccactttc tggtggcgat
     1261 gctaacagag ctgtcgcgct ggttgcaata ttgtcgttct ttattggctt agtaagcatt
     1321 attagtgtga ttgcacgcat tggttttgtg gctgagtttt tgtcaaggcc aatactccta
     1381 ggctacatgg caggtctggc acttg
//
LOCUS       DAOPLS010000217         1396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_112389_length_1396_cov_3.789709, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000217 DAOPLS010000000
VERSION     DAOPLS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112389_length_1396_cov_3.789709"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PKV52_04230"
     CDS             complement(<1..1143)
                     /locus_tag="PKV52_04230"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPD99451.1"
                     /translation="MKIVITGGGTAGHVTPLLAVASEVKSLEPSVHIRYIGKYSDTTT
                     QSIVTNQAIEKNYAIAAGKLRRFHGKKWYWYFTQPGLVARNIKDSILFSVGFVQSISI
                     LIFWRPNVVFVKGGYVGLPVGLAAACLRIAIVTHDSDVLPGLTNRILSKFARTLAVGA
                     PVDQYPQYANKTVVYTGVPVRNDFIQPPLKSAARKQLGIPKQSHVVAIVGGSLGAVRV
                     NDAILVNVDKLFVDTFKSPDSPLKLLWFTGAHGYKKILHAVEKSSHKDNIIVEPYTND
                     LATVFSAADVVITRAGATTLAELSIMKKPSVIIPNPLLTGGHQTMNARVFQQNNAAIV
                     VTEKQLADSPLILSGLVKDVLMNQEKQLMLSDNMSTFARVDAAKDIA"
     gene            complement(1076..>1396)
                     /locus_tag="PKV52_04235"
     CDS             complement(1076..>1396)
                     /locus_tag="PKV52_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPD99452.1"
                     /translation="QRAPNRTMQLMSAGVFTWFAVQGTINIAAMLGLIPLKGITLPFI
                     SYGGTSLIFSMAALGIVFHISKYTLMRKATLALPKQEGDAREDSYHWRRDSRPRYTVT
                     RRSL"
BASE COUNT          398 a          318 c          276 g          404 t
ORIGIN      
        1 tgcaatatct ttagcggcat ctactctggc gaatgttgac atattatcag ataacatcaa
       61 ttgtttctct tggttcatta atacatcttt tactagacca cttagtatca agggagaatc
      121 tgcgagctgt ttttcagtta ctactattgc tgcattgttc tgctgaaata cacgtgcatt
      181 catcgtttga tgcccaccag ttaagagcgg atttggtatg ataacacttg gttttttcat
      241 tatagaaagt tcagcgagtg tggttgcacc agcccgcgtt atcacaacat cagctgccga
      301 aaatactgta gctaagtcgt ttgtgtacgg ttctactata atattgtctt tgtgagaaga
      361 cttttctact gcatgtaata tttttttgta gccatgcgct cctgtaaacc acaaaagttt
      421 taatggagag tctggacttt taaaggtatc tacaaacagt ttatccacat ttaccaagat
      481 agcatcatta acccgaacag cacccaaaga tccgcctaca atggcaacca catggctttg
      541 tttaggaata ccaagctgct tacgcgcagc agattttaac ggtggttgta tgaaatcatt
      601 gcgcacaggc acacctgtgt aaacaaccgt tttattcgca tactgtggat attgatctac
      661 cggagcccca acagctaacg tgcgtgcaaa ttttgataag attcggttag taaggccagg
      721 taggacatct gaatcgtgcg ttacaatggc aatacgcaag caagcagcag ccagcccaac
      781 gggcaaacca acgtatccac cttttacaaa aaccacgtta ggacgccaaa atataaggat
      841 actaattgat tgtacaaagc ctacgctaaa caatatacta tcttttatat ttctggcaac
      901 cagcccaggt tgcgtaaaat accaatacca ttttttacca tgaaaacgcc gtaacttacc
      961 ggcagcgata gcgtagtttt tttctatcgc ctggtttgta acgatacttt gcgtcgtagt
     1021 atcgctatac ttcccaatat agcgaatatg cacactcggt tctagggact taacttcaga
     1081 ggctacggcg agtaacggtg taacgtggcc ggctgtcccg ccgccagtga taactatctt
     1141 cacgcgcatc cccttcctgc tttggtaaag ctagagtagc ctttctcatg agtgtgtact
     1201 tagatatgtg aaacactatg cctaacgctg ccatagaaaa aatcaagctt gtcccaccgt
     1261 aacttataaa aggcaatgtg atgcctttta aaggtattaa gccaagcatt gcagctatat
     1321 taattgtgcc ctgcaccgca aaccacgtaa acacaccagc gctcattaat tgcatcgtac
     1381 gattaggggc tcgctg
//
LOCUS       DAOPLS010000218         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_113304_length_1389_cov_2.734633, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000218 DAOPLS010000000
VERSION     DAOPLS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113304_length_1389_cov_2.734633"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..463)
                     /locus_tag="PKV52_04240"
     CDS             complement(23..463)
                     /locus_tag="PKV52_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99453.1"
                     /translation="MREKLAYVLTLNKNDFQGSDDPLLRKLVAMLEKLPPVVVEDALH
                     EYSLFPPSNFFSQGHLMALVQKHLALYMRLPTVEKSWEAIQFYKRRYGAGQRIPIDPT
                     IDELIEYFGGWAYVVDNINEYTWTKAYAQKIDTITKNKIMSITQ"
     gene            complement(460..636)
                     /locus_tag="PKV52_04245"
     CDS             complement(460..636)
                     /locus_tag="PKV52_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99454.1"
                     /translation="MTEYNIVCFENHKWCVKNIQTRRIVRRFDTKDALIAYLIDNKTL
                     LWAKRSLAFISNIK"
     gene            complement(633..1130)
                     /locus_tag="PKV52_04250"
     CDS             complement(633..1130)
                     /locus_tag="PKV52_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99455.1"
                     /translation="MLKEYTEFTNTTAMYPRNNVIMGLDYLALKLSGETAEYLVAETK
                     TDTMLEIGDLFWYTAQIYHVLDIDMQLVWGIIENTRAMFAFDQYDLVTITGQISETVG
                     KAIRKGEFTSVTETRLTHYSFMLLACLYTLLEAGNFTLAEVLDGNVAKLTGRVATNTI
                     SEEAR"
     gene            complement(1140..>1389)
                     /locus_tag="PKV52_04255"
     CDS             complement(1140..>1389)
                     /locus_tag="PKV52_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99456.1"
                     /translation="LVDTHLQKTNRWIVTQLGAGASIYKSSKTSARKISMVAGEHLLL
                     LNDIDTTDASEYNIKYEWYIKETWQIIDKIKPPQAALW"
BASE COUNT          386 a          291 c          236 g          476 t
ORIGIN      
        1 accgcctttt gactttcgat actcattggg ttatgctcat gattttgttt ttagtgattg
       61 tgtcgatttt ttgggcatat gcctttgtcc atgtgtattc atttatattg tcaacaacat
      121 atgcccaacc gccaaaatat tcaataagtt catcaatagt aggatcaatc ggtatacgtt
      181 gccctgcacc ataacgtcgc ttataaaatt gaatcgcttc ccaacttttc tcaacagtgg
      241 gcaaacgcat atatagcgcc aaatgctttt gtactaacgc cattaagtgc ccttgtgaaa
      301 aaaagttact aggtggaaat aaagaatact cgtgtaacgc atcctcaaca actacagggg
      361 gtaatttttc tagcattgca actaatttcc gcaacaacgg atcgtcacta ccctgaaaat
      421 catttttatt tagtgttagt acgtatgcta gtttttctct cattttatat tagaaatgaa
      481 tgcaagtgag cgtttcgccc atagtaacgt tttattgtca atcaaatatg caatgagtgc
      541 atcttttgta tcaaaacgac gaacaatgcg gcgcgtttgg atatttttaa cgcaccactt
      601 gtgattttca aagcaaacaa tgttatattc tgtcatcgcg cttcctcact gattgtattt
      661 gtggctactc ggcctgttag tttagccacg ttcccatcta aaacttctgc aagggtaaaa
      721 ttaccagctt ccaataatgt gtataaacat gctaataaca taaaactgta gtgggttaac
      781 cgtgtttcag ttaccgatgt aaattcgccc ttacggatcg ctttaccaac ggtttcactg
      841 atttgccctg ttatcgtgac caaatcatac tggtcaaatg caaacatagc acgggtattt
      901 tcaataatac cccacaccaa ctgcatatct atatctaata catggtaaat ctgtgccgtg
      961 taccagaaca aatcaccaat ttctaacata gtgtcagttt ttgtttctgc cactaaatat
     1021 tcagcagttt ccccactaag ttttaatgcc aaatagtcta gccccattat tacattgtta
     1081 cgcgggtaca ttgccgttgt attcgtgaat tctgtgtatt cctttaacat atcaatcctt
     1141 taccacaagg ctgcttgtgg tggtttaatt ttatcaatga tctgccaagt ttccttgata
     1201 taccattcat atttgatatt gtactcgctt gcatcggtgg tgtcaatatc atttagtaac
     1261 aataaatgtt ctcccgctac cattgatatt ttacgtgcgc tagtctttga tgatttgtag
     1321 attgatgcac ctgcgcctag ctgtgtaaca atccatctgt tggttttttg taaatgagta
     1381 tcaaccaag
//
LOCUS       DAOPLS010000219         1388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_113408_length_1388_cov_3.026257, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000219 DAOPLS010000000
VERSION     DAOPLS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113408_length_1388_cov_3.026257"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKV52_04260"
     CDS             <1..354
                     /locus_tag="PKV52_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99457.1"
                     /translation="IHVKPFGRIQITDYKGNTVEQYEFNNTEPKSNVLPNSTRKFTDE
                     VNYSNFFGKYTITANLGYSSAGNLITAKNTFWVVPVWLVVVLAAALLVVLVGAFLLYR
                     KLRQSRKHKVNPRRK"
     gene            458..1351
                     /locus_tag="PKV52_04265"
     CDS             458..1351
                     /locus_tag="PKV52_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99458.1"
                     /translation="MTYNRLAQLTTAVIGSGILLIMVAVTAFAQVENPQNGGIGLQGK
                     ISSPPPSSAPTISTPGNGQVISTIPIEVRGICTGNLLVKLYKNNVFSGSDQCRNGSYS
                     ITIDLFSGRNELVARHVDELDQQGPDSNVVAITFNDGASRPNLAERITVTSSYARRGA
                     NPTETLSWPIVITGGTAPYALSVDWGDGSQADVYSITIPGEFTIKHVFKQAGVYRALI
                     KATDKNGNVGFLQLTAIANGEIKQADVAGAVESGRTITKVLWQPVVLAIPFVITTFYL
                     GKKYEVTRIKQKLSKGEHPFD"
BASE COUNT          434 a          271 c          303 g          380 t
ORIGIN      
        1 atccacgtta aaccttttgg ccggattcag attactgact ataaaggtaa cactgttgag
       61 caatatgaat ttaataatac cgagccaaag tctaatgttc tgccaaatag cactcgtaaa
      121 tttacagacg aagtaaacta tagtaatttc tttggtaaat atacgataac cgctaactta
      181 ggctatagtt cagcaggtaa cctcattacg gcaaaaaata cattttgggt tgtaccagtt
      241 tggcttgttg tagtactagc tgccgcactc ttagtagtgc tggttggtgc gttcttgctt
      301 tatagaaagc ttcgccagag tcgtaaacac aaagttaatc cacgacgtaa ataacacata
      361 atgggttttc tgcaataagg ggcgatatag tgcccctttt tgctttaatt cagatggcat
      421 agtacactta tagataagta atgtaagtaa aaaaacaatg acatataaca gattggcaca
      481 actaacaacg gcagtcatag gtagtggtat attgttgatc atggtggctg tcacggcttt
      541 tgcgcaagtt gaaaaccccc aaaatggtgg catcggcttg caaggcaaaa taagctcacc
      601 accacctagt agcgcaccaa ctatatctac gcccggtaat gggcaggtga tatctacgat
      661 acctatagaa gtccgtggta tctgtacagg taatttgttg gtaaaactgt ataaaaataa
      721 tgtgtttagc ggttctgacc aatgtaggaa cggtagctat agcattacaa ttgatctttt
      781 tagtggcaga aacgaacttg tggctcggca tgtagacgaa cttgaccagc agggcccaga
      841 ttctaatgtg gtggcaatta catttaacga tggtgcctcg agaccaaatt tagcagagcg
      901 tataacagta accagttcat acgcacggag gggtgcaaac ccaacagaaa cattgtcttg
      961 gcctattgtc attactggcg gtaccgcacc ctatgcactg agtgtagact ggggtgatgg
     1021 cagccaagct gatgtgtatt caataaccat tccaggtgag tttacgatta aacacgtgtt
     1081 taagcaggca ggtgtatacc gagcgcttat taaagcgact gataaaaacg gcaacgttgg
     1141 ctttttacaa cttaccgcaa tcgcaaacgg cgaaatcaaa caggcagatg tcgccggtgc
     1201 ggtcgaaagt ggtaggacaa tcacaaaagt actatggcaa cctgttgtac ttgctatacc
     1261 gtttgttata acaacgtttt atctaggtaa aaaatacgaa gttacccgta ttaagcaaaa
     1321 actttctaag ggtgaacacc catttgattg ataaatctgc tacactatac atctagtgaa
     1381 aacagttt
//
LOCUS       DAOPLS010000220         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_113793_length_1385_cov_2.457895, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000220 DAOPLS010000000
VERSION     DAOPLS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113793_length_1385_cov_2.457895"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1385
                     /locus_tag="PKV52_04270"
     CDS             <1..>1385
                     /locus_tag="PKV52_04270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD99459.1"
                     /translation="SFKVWSTAKRVVFTDKAYLHYRQDNEASSVNSPGKVFCVADEYA
                     EIEKFLKKHKLWGKFAALVQKTKYGAYSWNFKRLTEGLDAEFLELFSKQYKEVYESSV
                     LDPFYFDINERRDLREIIYNPKMFLARKQAKKAAKVSVIVPVYNVEKYLPKCLDSLIG
                     QTMKEIEIICVNDGSPDDSISILEQYHAKDPRITIRETTNRGLAAARNHGMAQASGDY
                     LMFCDSDDYYSLDACETMYNTITKNKVDIAVAGIDVDYSTVELARRYKHPDTEYFRLR
                     NNGLHKVTKELIQSTAVSAWNKIYSTPLQRKYQIWFPNGLWYEDLTFFMNYMLVSKKA
                     YFLRNQKIYIYARRDGSIMSTTASKTPKAIDHVKATIQTFWFMKRHNLLANNVELFTE
                     TFAQNYMLSIRHMPQSDHSKLYELVRGFVSTNKPYLNSIDPEMVAKLSKLPPKRSFAE
                     KVDAKIRPKVS"
BASE COUNT          446 a          308 c          286 g          345 t
ORIGIN      
        1 tagctttaaa gtgtggtcaa ccgcaaagcg tgtcgtattt acagacaagg cttacttgca
       61 ctatcgccaa gacaatgagg catcttctgt aaatagccct ggcaaggtat tctgcgttgc
      121 agatgaatac gccgagattg aaaagttcct taaaaagcac aagctatggg gcaaatttgc
      181 ggctcttgtt caaaaaacaa aatacggtgc ctacagctgg aacttcaagc gcctaactga
      241 aggcttagac gccgagttct tagagctatt ttcaaaacag tacaaagaag tgtatgaaag
      301 ctctgttttg gacccatttt attttgatat taacgagcgg cgtgacttac gcgagattat
      361 ttataatcca aaaatgttct tggctcgtaa gcaagccaag aaagccgcta aggtgtccgt
      421 gatagttccc gtatataacg ttgaaaaata cctgcctaaa tgcctagata gtctgatagg
      481 gcaaaccatg aaagagattg agataatctg tgtcaacgat ggctcgccag acgattctat
      541 atctatacta gagcagtacc acgctaagga cccgcgcata accatccgcg aaacaaccaa
      601 ccgcggtctt gctgcagcgc gcaaccacgg catggctcaa gcatctggag attacttgat
      661 gttttgtgat agtgacgact attatagtct tgatgcctgc gaaacaatgt ataacaccat
      721 aactaagaat aaggttgata ttgccgtagc cggtatcgac gtagactact ctacggtaga
      781 gctagctagg cggtacaaac acccagatac tgaatacttc cgtctgcgaa acaatggtct
      841 gcacaaggtt accaaagagc taatccaatc aaccgccgtg tcggcttgga ataaaatata
      901 ttcaaccccg ctgcagcgaa agtatcagat ctggtttcca aatggcctat ggtacgaaga
      961 tctaacattc tttatgaact acatgctagt cagtaaaaaa gcctatttct tacgcaacca
     1021 aaagatatac atctacgctc ggcgtgatgg cagtataatg tctacaacag ctagtaaaac
     1081 gccaaaggcc atagatcacg taaaggctac catacagact ttttggttta tgaagcgaca
     1141 caatctatta gctaataatg ttgaattatt taccgagaca ttcgctcaaa actacatgct
     1201 ttcgattaga catatgccgc agagcgacca ctcaaagctc tacgaacttg tacgaggatt
     1261 tgttagtacc aacaaaccct acctgaactc tatagaccca gaaatggttg ccaagctcag
     1321 taagctaccg ccaaaacgct cttttgcaga aaaggtggat gcaaaaatta gaccaaaagt
     1381 atcaa
//
LOCUS       DAOPLS010000221         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_115670_length_1369_cov_3.688737, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000221 DAOPLS010000000
VERSION     DAOPLS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115670_length_1369_cov_3.688737"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..858
                     /locus_tag="PKV52_04275"
     CDS             148..858
                     /locus_tag="PKV52_04275"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI family restriction endonuclease"
                     /protein_id="HPD99460.1"
                     /translation="MNATIFEEFLQKVDLNKYRIKYQPIKLVEMDMPRNIQALDALYE
                     VYWTQRRVLGFEDFYDYYKNSCTKEIEDFRQKTTMCSECFYRGLPARIYRTWASIITQ
                     IHAGYVAEQVFGSGSVSMSTELDHKGIDFSAQVDGTYLYYQVKKATHSREVRVSKKVD
                     SQNIDIILIEYEVPGAAVFAAPYKRDGTYKAAYSRFIQNKSIKRLPNGFVVFTPYMFE
                     QKKAELLAGGGASYKAFR"
     gene            complement(817..>1369)
                     /locus_tag="PKV52_04280"
     CDS             complement(817..>1369)
                     /locus_tag="PKV52_04280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012359182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPD99461.1"
                     /translation="HFWQRSHESIIVAYKNKPLFNRDAVREPYTEGFLKGAAGKVRKN
                     TKGRFSKGGGEPTTYNAHESGALPRDVIKIPALAGGAGMSERWFVCRTCNSTVFTPQV
                     FKQHREHDYLKHPTQKPIELTKRLLLSAMPKDGGLVLVPFVGSGSECYVAKQLGFDFI
                     GYELNPEYAVLAEGFIAGTATSK"
BASE COUNT          416 a          264 c          278 g          411 t
ORIGIN      
        1 taaaaaacaa catcctaaaa accctggata cattagtgtg ccaatactgc tgccatggtt
       61 gggagtgata agctgcgcgg gtatactcgt ctatcaactt agtacaatat tttaataatt
      121 cattcatagc atatatagtg ataggatatg aacgcaacca tttttgaaga atttttgcaa
      181 aaagtagatc ttaataaata tagaattaag taccaaccaa ttaagcttgt agaaatggat
      241 atgccacgca atatacaagc tttagacgcc ttgtatgaag tgtactggac tcaacggcgc
      301 gtgcttggtt ttgaagattt ttacgattac tataaaaata gctgcacaaa agaaatagaa
      361 gactttaggc aaaaaacaac aatgtgttcg gaatgttttt acagaggttt accagccaga
      421 atttaccgta cgtgggcaag catcattacc cagatacacg ctggctatgt agctgaacaa
      481 gtatttggga gcggtagcgt gagtatgtca actgagctag atcacaaagg tatagatttt
      541 tcagcacagg tagatggcac atacttatat tatcaagtta aaaaagctac ccacagccga
      601 gaagtgcgag tttctaaaaa agttgatagc caaaatatag atattatact cattgaatac
      661 gaagttcctg gagccgcagt ctttgcagca ccttataaac gtgatggtac gtataaggct
      721 gcttattcgc gttttataca aaataagtct atcaaacgct tgcctaatgg ttttgttgtt
      781 tttactccct atatgtttga gcaaaaaaaa gctgagctac ttgctggtgg cggtgccagc
      841 tataaagcct tccgctaata cggcatattc aggattaagc tcgtagccta tgaagtcaaa
      901 acctaactgc ttagcgacat agcattcaga accagaccca acaaatggca ccagcactaa
      961 gccgccgtct tttggcattg cagataacaa taatcgttta gtaagttcta ttggtttttg
     1021 tgttggatgt tttaggtaat catgttcacg atgttgttta aaaacttgag gcgtaaacac
     1081 tgtgctgttg catgttctgc atacgaacca tcgttcgctc atgcctgctc cacctgcaag
     1141 tgctggtatt ttaataacat cacgtggtaa tgctccagat tcatgtgcat tataggtggt
     1201 aggctctccc ccgcctttgc tgaaccggcc ttttgtgttt tttcttactt taccggcagc
     1261 accttttaag aaaccttcgg tatacggttc acgtacagca tctctgttaa ataacggttt
     1321 atttttatag gcgacaatga tagattcgtg actgcgttgc caaaaatgt
//
LOCUS       DAOPLS010000222         1354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_117655_length_1354_cov_2.916089, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000222 DAOPLS010000000
VERSION     DAOPLS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117655_length_1354_cov_2.916089"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..765
                     /locus_tag="PKV52_04285"
     CDS             151..765
                     /locus_tag="PKV52_04285"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99462.1"
                     /translation="MKYSICVSGAAAGKTVEQSKYAAEKVGRAIAKSGHIITTGATVG
                     LPFYAARAAHRAHGASIGFSPATSLREHVMKYRLPIGMFDFINFTGLNYVGRDTYMVQ
                     SSDAVITIGGRFGSLHEFATAIENGTPCGVLTGTGGTADVLPDLIEKLEVPHKSSIIF
                     DSDPESLVRRMIEKLDVEIGEIDTSAIANEWFLHGDEEVTKRAG"
     gene            768..>1354
                     /locus_tag="PKV52_04290"
     CDS             768..>1354
                     /locus_tag="PKV52_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99463.1"
                     /translation="MPKSTTKKSPNATIAQQRRSKTPERIYKVKPKKQVIKPRPKIKR
                     RHHKHKQLLSMSLPRVATIVIIVFVTTLASGIVFVRAYQAYNALLSANESASDALYSK
                     EMVEKMRLIGDGTNYPNEIINYQSAPQDLQNFLVQDYKKLKTGCIINGQFAGTLQYTV
                     VNVVYDSFARIEKNCNGADTLLLSKISGVWTVIYAG"
BASE COUNT          433 a          261 c          306 g          354 t
ORIGIN      
        1 agtatttcac tttagagaag ccctttggaa tgaattattt atgcgctata tgggcgaaaa
       61 aaccctcgaa cagcaagtac tgggtcaact aaacaatagt ttaattgccc cagaaaccat
      121 tcatgcatct gtgaaaggga ggggttttta gtgaagtatt ctatttgtgt ttctggtgct
      181 gctgcaggta aaaccgttga gcaatcaaaa tatgccgcag aaaaagtagg tcgggccata
      241 gcaaaaagtg gccacataat cactacaggc gctacggtag gcttaccttt ttacgcagca
      301 cgagcagccc atagggccca tggtgctagt ataggttttt caccagccac atcattaagg
      361 gaacatgtga tgaaatatcg actacctata ggcatgtttg acttcattaa ttttactggg
      421 cttaactatg taggtagaga tacatatatg gtgcaatcat cagatgcagt tatcacaata
      481 ggtggtagat ttggcagttt gcacgagttt gctaccgcta tagaaaatgg tacaccgtgc
      541 ggtgttctta ctggcacagg tggcaccgca gatgtattgc cagatttaat agaaaagctc
      601 gaagtaccac acaaaagtag cataattttt gatagcgatc cagaatcatt agttagaagg
      661 atgatagaaa agttagatgt tgaaattgga gagattgata cttccgctat tgcgaacgag
      721 tggtttttgc atggtgatga agaggtaacg aaacgagctg ggtaactatg cccaagtcta
      781 caactaaaaa aagccctaac gctaccatag cacagcaacg gcgctccaag acgccggagc
      841 gtatctataa agtaaaacca aaaaaacaag taataaaacc ccgcccaaaa ataaaacgtc
      901 ggcaccataa acacaagcag ctgctatcta tgtcgctgcc ccgggtagct acgattgtta
      961 tcatagtatt cgttacgact ctagcgagtg gtattgtttt tgtacgtgcg taccaagcat
     1021 acaatgcgct gctttctgcc aacgaatcag cgtcagacgc attgtatagt aaagaaatgg
     1081 tagaaaaaat gcgtctcatt ggcgatggca ctaactaccc aaacgaaatt attaattatc
     1141 agagtgctcc gcaagatttg cagaattttt tggtacaaga ttataaaaag ctcaaaacag
     1201 gctgtattat taatgggcaa ttcgctggca cgctacagta tacggttgtc aatgttgtct
     1261 acgattcatt tgcacggata gaaaaaaact gcaatggagc agacacgttg ctactatcta
     1321 aaattagtgg cgtatggacg gtaatatatg cggg
//
LOCUS       DAOPLS010000223         1351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_118036_length_1351_cov_4.698302, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000223 DAOPLS010000000
VERSION     DAOPLS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118036_length_1351_cov_4.698302"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PKV52_04295"
     CDS             complement(<1..640)
                     /locus_tag="PKV52_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPD99464.1"
                     /translation="MKISDYNYDLPEDKIALQPVKIRGTSNLLVLHKKSGAIEDKKYS
                     DIVDYLQNGDVLVLNDTKVIKARLNVTKQNGAKRELIILEKHGINDDWHTHKIMYRRK
                     LAVGDVLTVGKATITVNKILGDGLAIVQSSMDLLDVAEQYGSVPLPPYIHRDVKESDT
                     ERYQTVWAKEQGSVAAPTASLNMTDDTLANLQSKGIHVVYATLHVGLGTFMPI"
     gene            complement(719..1267)
                     /locus_tag="PKV52_04300"
     CDS             complement(719..1267)
                     /locus_tag="PKV52_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99465.1"
                     /translation="MKKIFFTAALVLAIAGGLLLWGGVYASNFVKENLADQKITFPSS
                     EALTAQGRDDLVSYAEATVDTGDEAKAFASYIQGHIAKIANGQTYSEVSGQYQALTPE
                     QKSTPEGEKLAEQRLTIFMGETLRGTLLNAYGWGFVAKIVLLAGAGLLAAAAVAATLF
                     VLGNISSLPQKSAKKKTTKRRK"
BASE COUNT          339 a          297 c          259 g          456 t
ORIGIN      
        1 ggataggcat aaacgtgcct aacccaacat gaagcgttgc gtataccaca tgaatgccct
       61 tgctttgtaa atttgctaag gtatcatctg tcatattgag gctcgctgta ggtgccgcga
      121 cagaaccttg ttcttttgcc catacagttt ggtaacgttc ggtatctgat tcttttacat
      181 ccctgtggat atatggtggc agtggcacag aaccatactg ctctgccacg tcaagtaagt
      241 ccatactact ttgcacaatc gccaaaccat cacccagaat tttattaact gttatcgtgg
      301 ctttgcccac agtaagtaca tcgcctacag ctagttttct acggtacatt attttatggg
      361 tgtgccagtc atcattaata ccatgctttt ctaatattat taattcgcgc tttgcgccat
      421 tttgcttagt gacgttcaag cgggctttta tgacttttgt gtcatttaat acaagtacat
      481 caccgttttg caaatagtct acaatatcgc tatatttttt atcttctatc gcgccacttt
      541 ttttgtgcag cactaataag ttcgatgtgc ctctgatttt tactggttgc aatgctattt
      601 tatcttctgg taagtcgtag ttgtaatcag aaattttcat actaggtaat agtaacagat
      661 tagcgctata aataaagccc cgcagtaaac ggagctatat ttcgttatgt gcttaatact
      721 atttacgtcg cttggtagtt ttcttttttg cagatttttg cggtaaagaa ctaatattac
      781 cgagcacaaa taaggtagcc gctacagcag cagctgcaag tagtccagcc ccggcaagta
      841 agacgatttt agctacaaaa ccccatccgt aggcattcaa tagtgtgccg cggagtgttt
      901 cgcccatgaa tatagtcaat cgttgctctg ctaacttttc accctctggt gtagattttt
      961 gttctggcgt taacgcctga tactgaccag atacttctga gtatgtctgc ccgttagcta
     1021 tttttgcgat gtgcccttgt atgtagctcg caaaggcctt ggcttcatcg cccgtatcta
     1081 cagtagcttc agcataactg actagatcgt ctctcccctg cgcagtaagc gcttcgctag
     1141 aagggaaagt tattttttga tctgctagat tttctttcac aaaattagac gcatagacac
     1201 ctccccacag gagtaatccg cctgcaatgg cgagtacaag tgccgctgta aaaaatattt
     1261 ttttcatatt ttttatattt tcttacttat tgattgctat taatggcaag tatacataag
     1321 cgttgctccg ttgtaaatac agcttacttt c
//
LOCUS       DAOPLS010000224         1343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_119097_length_1343_cov_3.850932, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000224 DAOPLS010000000
VERSION     DAOPLS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119097_length_1343_cov_3.850932"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKV52_04305"
     CDS             <1..847
                     /locus_tag="PKV52_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD99466.1"
                     /translation="INQQHLATLNTKAATYEMLLTGSKKYKEQLLKSCLAMPTLTLKI
                     NAQVMCIKNSPDKKYVNGSLGVVVGFEDTTNYPIVALNSGKKIVIKPESWELIDGDKI
                     RATATQIPLRLAWAITVHKSQGMTLDAATIDLSKAFVEGMGYVALSRVKSIDTLSLLG
                     INGMALKVSPVAKQLDEELRLKSKQAIQEYQHHINEWGKTKPLKTEILTKKQQGSWAD
                     KLAKMRITHPNAYKPWSEADDKKLVTQFSDGLSIPQLSKALGRHEGSIKIRLQKHLGE
                     DIFDA"
     gene            913..>1343
                     /locus_tag="PKV52_04310"
     CDS             913..>1343
                     /locus_tag="PKV52_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPD99467.1"
                     /translation="MASTDHNYAFIDSQNLNLGVKSAGWDLDFRKFRLYLKNKYNVKR
                     AYLFIGQVEGNELLYENLHEMGYELILKPTTEYTVDEVVTVKGNVDAELVLYAAAKVY
                     NLYDKAIIVSGDGDFYCLIEYLEQKEKLLHVMVPNDKYSKLL"
BASE COUNT          478 a          241 c          281 g          343 t
ORIGIN      
        1 tattaatcaa cagcacctcg cgacccttaa caccaaagca gctacatatg aaatgctctt
       61 aactggcagc aaaaagtaca aagagcaact acttaagtct tgccttgcca tgcctacttt
      121 aacattaaaa attaatgctc aagttatgtg tattaaaaat agcccagaca agaaatatgt
      181 caatggaagt ttaggcgtcg ttgttggttt tgaagatacc acaaactacc caatagttgc
      241 gcttaatagt ggtaaaaaaa tcgttattaa accagaatcc tgggagctta tagatggaga
      301 taaaataaga gctacggcga cacaaattcc cctacgtttg gcatgggcga taactgtcca
      361 caaaagccaa ggcatgacgt tagatgccgc aactatagat cttagcaaag catttgtaga
      421 aggtatgggc tacgttgctt tatcgcgcgt aaaaagtatt gatacgttgt cattgctggg
      481 aattaatggc atggcgctta aagtaagccc cgttgcaaaa caactcgatg aagaactccg
      541 tttaaaaagc aaacaggcaa ttcaagaata ccagcatcac ataaacgaat ggggtaagac
      601 gaagccgctt aaaaccgaga tacttaccaa gaaacaacaa ggtagctggg cagacaaatt
      661 agcaaagatg cgtattacac accctaacgc ctacaagcca tggtcagagg ctgacgataa
      721 aaaactagtg acgcagttta gcgatggact atcaataccg caactaagca aagctttagg
      781 gaggcacgaa ggatctataa aaatacgcct tcaaaaacat ttaggtgaag atatatttga
      841 tgcctgattt tctataaaac atttgcgcat gatgcgtgca aatatcaaaa caggagcgta
      901 ctataaggag taatggcaag tacggaccat aattacgcat ttatagacag tcaaaacctt
      961 aatctcggtg tgaaaagtgc tgggtgggat ttagatttta gaaaatttcg tttgtattta
     1021 aagaataaat ataacgtcaa acgtgcgtat ttatttattg gtcaggtaga gggcaatgaa
     1081 ttattgtatg aaaatttgca tgaaatgggc tatgagctta tattaaagcc aacgacagaa
     1141 tatactgtag acgaagtagt gacggtaaag gggaatgtag acgccgaact cgtgttgtac
     1201 gctgcggcga aagtatacaa tctatacgac aaagccatca ttgtatctgg tgatggtgat
     1261 ttttactgct taatagaata cttagagcaa aaagaaaaac ttctccatgt tatggttcca
     1321 aacgataaat actctaagtt act
//
LOCUS       DAOPLS010000225         1325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_121644_length_1325_cov_3.850394, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000225 DAOPLS010000000
VERSION     DAOPLS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121644_length_1325_cov_3.850394"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1325)
                     /locus_tag="PKV52_04315"
     CDS             complement(<1..>1325)
                     /locus_tag="PKV52_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99468.1"
                     /translation="SQLAAVYKTLSKNVGGASIQLDQLDFPVDKVEKASKGIGKLGEA
                     IKYKLINYLEQGVRAAVNFFRTLSDEAVKTAEEFKVTQIAFEQILGDPQAALATFNLI
                     RRESERAGADITGLVAQLLPSLADPAVQIPQLATIAQGLSRTQAAILQGRDATDATIA
                     LTELFAGGQTKSLKERYNFDPVVLREVLELIRTEGQAAGADRLIEYLGELGLLGEDLT
                     NTYTQTSARLKSFYTTLKGEIGTPINDALLVFFQQLSEFIAENRDDILALGFALGEAL
                     TTLLQFASTNILDNLNTDTLTSAYNSLLDFINLLTRLSVMTQGGIFNLQDFNLVEAIV
                     NYIIDDVEDFIVLLSRIFGFKYDDSALAKAFEKIREYVTGVFDEYGSAEKLLQTGQQL
                     VAIIKYLFGAVVSAASVMVKAIKDVNSFLADPFGNKDIFDDYGTQVLDQ"
BASE COUNT          386 a          277 c          245 g          417 t
ORIGIN      
        1 aattgatcta atacttgtgt gccgtaatca tcaaagatgt ctttattacc aaatgggtca
       61 gctaaaaaac tattaacatc tttaatagct tttaccataa ccgatgcagc tgacacaaca
      121 gcgccaaata aatatttgat aatggcaact aactgttgcc cggtctgtag taacttttca
      181 gcagagccgt attcgtcaaa aacacctgta acatactcac gaatcttttc aaatgctttt
      241 gctagtgcgc tgtcatcata tttgaagccg aaaattcgtg ataagagtac aataaaatct
      301 tcaacatcgt caattatata gttaacaata gcttctacaa ggttgaagtc ttgaagatta
      361 aaaatgcccc cctgtgtcat tacagaaaga cgtgttaata aatttataaa atctaacaac
      421 gaattataag cacttgttag tgtgtcggta tttagattat ctaaaatatt tgtacttgca
      481 aactgaagta atgttgttaa cgcttctcca agtgcaaagc ctagtgccaa tatatcatca
      541 cgattttcgg caataaattc actcagttgt tggaagaata ctaacaacgc atcgtttatt
      601 ggcgtaccaa tttcaccttt cagtgttgtg taaaatgact tcaatctggc actggtttgg
      661 gtataggtat tagttaaatc ttcacctaac aaccctaatt cacccaagta ttcgatcaag
      721 cggtctgcac ctgctgcctg tccttcggtg cggataagtt ctagcacttc acgtaatact
      781 accgggtcaa agttataacg ctcttttaac gattttgttt gcccaccggc aaacaattct
      841 gttaaagcaa ttgtagcatc tgtggcgtca cgtccctgta agatagcggc ctgtgtacgt
      901 gataacccct gtgcgattgt agccaattgg ggtatttgta cagcggggtc tgccaaacta
      961 ggtaataact gtgctaccaa cccggtaata tcggctcccg cacgttctga ctcacggcgg
     1021 attaaattga atgttgctag tgctgcttgc ggatcgccta agatttgttc aaaagcaatt
     1081 tgtgtaactt tgaattcttc ggcggttttt acagcttcat cgctcaaagt acggaaaaag
     1141 ttaacagcag cacgcacgcc ctgttccagg tagttgatta acttatattt aattgcttcg
     1201 ccaagtttac caatcccctt agatgctttt tcaactttgt ctacagggaa atctagttga
     1261 tctaattgaa ttgatgcacc accaacattt ttacttagtg ttttgtatac ggctgcaagc
     1321 tgact
//
LOCUS       DAOPLS010000226         1325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_121689_length_1325_cov_2.573228, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000226 DAOPLS010000000
VERSION     DAOPLS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121689_length_1325_cov_2.573228"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1325)
                     /locus_tag="PKV52_04320"
     CDS             complement(<1..>1325)
                     /locus_tag="PKV52_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99469.1"
                     /translation="DGYPTVGNLRQEETRTLATLKAIAPALTDAMFIGYRYHNVSLMA
                     AMAKAAGGQLIHANNIHEVGEASERFLDNDTGRVTTPYPAEPVLAFQIEADHVEYVAW
                     FDGALHSKPGDVYVVEKAATFESCGVLASYATAALLAELGQADLALQVLGEIGDIHLI
                     DMLNNAILPQEIVRAKNAIVEAIFDQTTRFQNGYAAGYVPAPDAFCLLDLIRVLDDVN
                     AELVLDALEYHTIGRKGVSQTSITFKNLPGQTASFSDFVWNNERLNLSLRTYRRGQAS
                     FVFNGNDYVIPTGRWRTYTLIADGHPNVARLPVKVDAAGFAVLAKHFDLNSEDDVHFL
                     DLNTIPVINQMHQRPSALHLGRMVLNETMLMGRQTALNALLPKDKSASATLVAVYGQA
                     LADWLALEGLTESGFSPRSINAPTTDSYIVPTFKIAVKGMKSIPTVAVA"
BASE COUNT          374 a          314 c          258 g          379 t
ORIGIN      
        1 agctacagca acggtaggaa tggatttcat gcccttaact gcaattttga atgttggtac
       61 aatgtaacta tctgttgttg gtgcattgat actgcgtggt gagaaaccac tttctgttag
      121 tccttctagc gccaaccaat cggcaagtgc ttgtccgtaa acagctacga gtgttgcact
      181 tgcgctcttg tcctttggca ataacgcatt cagtgcggtt tgtcgcccca ttagcattgt
      241 ttcattcaat accatgcgcc ctaagtgcaa tgcgcttggc cgttgatgca tttgattgat
      301 taccgggatg gtgttcaaat ccaagaaatg cacatcatct tcggaattca aatcaaaatg
      361 ttttgccaaa accgcaaaac cagccgcatc aactttaacg ggcaaacggg caacatttgg
      421 gtgtccatcg gcaattagtg tataggtgcg ccaacggcct gttggaatta cataatcatt
      481 gccattaaag acaaatgacg cctgtccacg tcgatatgtg cgcaaactaa gatttaaccg
      541 ctcattattc cacacaaaat cactaaaact ggcagtttgt cctggtaaat tcttaaaagt
      601 gatacttgtt tgtgatacac ctttacggcc aatggtatga tattccaacg catctaacac
      661 caattcagca tttacatcgt ccagtacacg gattaagtct aacaaacaaa aagcatcggg
      721 cgctggcaca taaccagcag cataaccatt ctgaaaccgt gttgtttgat caaaaattgc
      781 ttcaacaatg gcattttttg cacgcacaat ttcttgtggc aaaattgcat tgtttagcat
      841 atcaattaaa tgaatatcac caatttcacc caaaacctgt aatgctaagt cagcttgccc
      901 taattccgcc aatagtgccg ctgtagcgta actagcgagt acaccgcatg attcaaaggt
      961 tgcagctttt tcaaccacat acacgtcacc cggctttgag tgtagtgccc catcaaacca
     1021 tgcaacatat tcaacatgat cggcttcgat ctgaaaagct aatacgggtt cggctggata
     1081 cggcgttgta acacgccccg tatcattatc taaaaagcgt tcacttgctt caccaacttc
     1141 atgaatattg ttggcatgta tcaactgtcc accagcggct tttgccattg ctgccattaa
     1201 actaacgttg tgataccgat agccaataaa cattgcatct gttaaagctg gtgcaatcgc
     1261 ttttagtgtc gccaaagtac gtgtttcttc ttgtcgtaga ttaccaaccg ttggataacc
     1321 gtcag
//
LOCUS       DAOPLS010000227         1324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_121807_length_1324_cov_2.936170, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000227 DAOPLS010000000
VERSION     DAOPLS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121807_length_1324_cov_2.936170"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKV52_04325"
     CDS             <1..227
                     /locus_tag="PKV52_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPD99470.1"
                     /translation="APKHESGNYLPVDSEECKDKVPPVTPPAPTTPTPKEQPIELPKT
                     GPEEAVVQIVGAMSLAGASSYYLSSRRRNN"
     gene            456..>1324
                     /locus_tag="PKV52_04330"
     CDS             456..>1324
                     /locus_tag="PKV52_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99471.1"
                     /translation="MKKKFIALLVMLLSISVLGVVQLQSQAAGHPNGKVGNAPVCDVK
                     AIGVRGDTSVDLASPAASALAFKLNGNTASVSFNVTGPKNCKVLLSARSFWAAAQDGK
                     PFNTQVLYNNTTKTFGRGTHSLAVNVPTTARNTMCYYQVDLTYGKYIVTPTLAYAHGM
                     VNSCKPPVPPTPQPVAKCDSLAVDKLSSTSFKLTANTTVKDGAQVTSYRFVIKKSGQV
                     VLDKTQNENTLTTEQTVAGEYAAQVYVNTNVGTFECANSFTVDKEKVPGIDIEKYVNG
                     MKQDSVAVDTPFS"
BASE COUNT          418 a          293 c          270 g          343 t
ORIGIN      
        1 cagcgcctaa gcatgagtca ggtaattacc taccggttga cagtgaggaa tgcaaagata
       61 aagttccacc cgtcacccca cctgcaccaa ctacaccgac tcctaaggag cagcctatcg
      121 agttaccaaa aactggccca gaagaagcag tagtgcaaat tgtcggggcg atgtctctgg
      181 ctggtgcaag tagctattac ctaagcagtc gacgacgaaa caactaaaaa atagaaatgg
      241 cttaaacgaa tcaatagaca gctatccccc aaacggtagc tgtctatttt tcaaatatac
      301 ccaagttcta ctgcttgtgc ttaagtatga ataattatat aaattattga cattttattc
      361 tttttgtgct aatatgtata taatcatctg cgagagatgg tgttgaactg tggaggttcg
      421 gctatataaa ccttatctac ctacgaggag gagatatgaa aaagaaattt atagcattac
      481 tggttatgct tttatctata tcagtacttg gtgtagttca gttgcaatca caagctgctg
      541 gacacccaaa tggcaaagtg ggcaatgcgc ctgtttgtga cgtcaaagca attggtgttc
      601 gtggtgacac gagtgttgat cttgcaagcc ctgcagccag cgcactagct ttcaagctaa
      661 atggcaacac agcaagtgtc agctttaacg taactggccc gaaaaactgt aaagtactac
      721 tttctgctag atcattctgg gcagccgcac aagatggtaa gccatttaac actcaagttc
      781 tgtacaataa cactacaaaa acatttggtc gtggtactca ttctctcgct gtaaacgttc
      841 cgactactgc tcggaatact atgtgctatt accaagtaga ccttacttat ggtaaatata
      901 ttgtaacacc tactctagcc tacgcacatg gtatggtgaa ctcgtgcaag ccacccgtgc
      961 caccaacacc acaacctgtt gccaaatgtg actcactagc cgttgacaag ttaagctcaa
     1021 ctagctttaa acttaccgca aacactacag tcaaagatgg tgcccaggtc acttcatacc
     1081 gatttgttat caaaaaaagc ggtcaggttg tgctagacaa aactcagaat gaaaacacac
     1141 taacaaccga acagactgtc gccggtgaat acgccgcaca agtgtatgtt aatacaaatg
     1201 ttggcacctt tgagtgtgca aatagcttca ccgtcgataa agaaaaagtt cctggtatag
     1261 atatagaaaa atacgtcaat ggaatgaagc aagacagtgt agctgttgat acaccgttta
     1321 gcta
//
LOCUS       DAOPLS010000228         1311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_123559_length_1311_cov_2.865446, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000228 DAOPLS010000000
VERSION     DAOPLS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123559_length_1311_cov_2.865446"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..478
                     /locus_tag="PKV52_04335"
     CDS             182..478
                     /locus_tag="PKV52_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99472.1"
                     /translation="MITKNSEYLSHCFGNWRAINYSSHLGFTESDESYAVPINIDFDW
                     CDTWDGLLWRFVANNQDVLSQNSRTASTVKTYKRLNEDRKRIISYRAQDFLNSL"
     gene            604..882
                     /gene="msrB"
                     /locus_tag="PKV52_04340"
                     /pseudo
     CDS             604..882
                     /gene="msrB"
                     /locus_tag="PKV52_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
     gene            1009..1272
                     /locus_tag="PKV52_04345"
     CDS             1009..1272
                     /locus_tag="PKV52_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99473.1"
                     /translation="MNETLLLAAAGPLMALCAMAIGFIIIEGETIVGGMLTTLGGMAL
                     LGSLLGYEAYPTAWAVSLFTGAVLAAMAMLENWHSTKKCQQRS"
BASE COUNT          389 a          273 c          315 g          334 t
ORIGIN      
        1 acaggaaacg atgcgaatgg caaaaaaatt cctcccatgt acagcaaata tgcaatcaaa
       61 caggctgaaa taaatggttc aatagtttgg tttgataagc ccatagagct ggtgggcaca
      121 attggatatt tcgatgcgaa agctggtaag gtaattaccc agccgaaata gttggcttgt
      181 aatgatcacg aagaacagtg agtatttgtc tcactgcttc ggcaattggc gtgctatcaa
      241 ttatagtagc catttgggtt tcaccgaaag cgatgaaagc tacgcggttc ccataaacat
      301 cgatttcgac tggtgcgata cgtgggatgg ccttttgtgg cgttttgttg caaataatca
      361 agatgtactg agccaaaact ctcgtactgc ctcaacggta aaaacctaca aacgtctaaa
      421 cgaagaccgc aaacgtatta tttcgtaccg cgcccaagat ttcttgaaca gtttgtagcc
      481 cgcgccgtat actaaaaggg catgaagcct acaaaactta caaaactgca aagagacgtg
      541 ctgatagaca agggcacaga accgccattt agcggtgatt actaatataa cgaagagtat
      601 ggcgaatacg tatgcgcagg ctgtggctcg gtactatttg caagtgaaca taaatttgat
      661 tctggcagtg gttggccaag tttttatgat gtcgccaata aaaaagctgt gcgtttagta
      721 gaagatactt caaacggtat gcatcgtgta gcggtagaat gtgccaattg cggtggccat
      781 ttaggccatg tatttaacga tgctccacaa acgcccacca atatacgttt ttgtattaat
      841 tcgtgcgtac tacattttaa cgaaaaagac atttctacct agagatataa aagcatacag
      901 ggaaaaccct ttacaaaaac ctattaaagt gctataatat aatacgttca tttcctggat
      961 gctgcatggt gcagaatacc gtccacgagc cttgtaaagg aggtgaaaat gaacgaaacg
     1021 ttgcttttgg cagcagcggg gcctctaatg gcgctctgcg ccatggcgat cggtttcatc
     1081 atcatagaag gtgaaaccat cgtcggcggc atgctcacca cgctcggtgg gatggcgctg
     1141 ctgggcagcc tgttggggta cgaagcatac ccaacagcgt gggctgttag cctgtttaca
     1201 ggcgcggttc tggctgcaat ggcgatgctg gaaaattggc acagtaccaa aaaatgccag
     1261 caaagaagtt aggttaaaaa acctcacccc tttgctggca ttttttggta c
//
LOCUS       DAOPLS010000229         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_125056_length_1301_cov_2.687801, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000229 DAOPLS010000000
VERSION     DAOPLS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125056_length_1301_cov_2.687801"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKV52_04350"
     CDS             complement(<1..664)
                     /locus_tag="PKV52_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99474.1"
                     /translation="MSRFISQLLGATEPMFTIAVQQLEQASGKPGVDVRLTAEIIGQI
                     HQKTADLRLDPKDTTGKELYAALLHRFKRDDEHLAKQIGGHNATDVQDLLPKMKAAAE
                     EVKIAKKCWVLKKSVAKEFLRKTPPPNIMKKLGYRSIDSMLKNENILEIYGALRFAES
                     PDWLNAFDAQYTDIKPSDFEERKIEIVTMPADRWADIAEPFVHKKRHNITHVKELGII
                     LML"
     gene            complement(680..>1301)
                     /locus_tag="PKV52_04355"
     CDS             complement(680..>1301)
                     /locus_tag="PKV52_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPD99475.1"
                     /translation="PSGERTILAYRGASAHYSIKTADFHDTKPEWIYLTSLDGNFEVL
                     HTVFAYAQQHNIKIAFNPGKKELAQKAELKKLIPKLTILSMNKQEMQSLYKGETLEEL
                     VRAANKDVHYVVVTDGPKGAMAADRWHIVKAGMYENVPVVDRTGAGDAFSSGFTAMIA
                     AGEPLEWAVTYASANSTRVVSKIGAKAGIIHIDDVVHEMPLKVKEM"
BASE COUNT          334 a          276 c          235 g          456 t
ORIGIN      
        1 gtagcattaa aataatacca agctctttta cgtgggtaat gttgtgtcgt tttttatgca
       61 caaaaggttc agcaatatct gcccagcgat cagcaggcat ggtaactatt tctatcttac
      121 gttcttcaaa atcagatggt ttaatgtctg tatattgtgc atcaaatgca tttagccagt
      181 ctggactttc tgcaaaccta agtgcaccat aaatttctaa aatgttttcg tttttaagca
      241 tactatcaat gcttctgtag ccaagctttt tcataatgtt aggtggtgga gttttacgaa
      301 gaaattcctt ggcaacactc ttttttagta cccaacattt ttttgctatt ttaacttctt
      361 cggctgcagc cttcatttta gggagtaaat cttgaacatc tgttgcgtta tggccaccaa
      421 tttgttttgc taaatgttca tcgtcacgct taaacctatg taataacgca gcatagagct
      481 ctttacctgt tgtgtctttg gggtcaagcc gcaaatcggc tgttttttga tgtatttgcc
      541 caataatctc ggcagttaat cgtacatcaa caccaggttt accactggct tgttcgagtt
      601 gttgtactgc aatagtaaac ataggctctg tagcaccaag taattgtgat ataaatctag
      661 acattaccca cccccatgtt tacatttctt ttacttttag tggcatctca tgtacaacat
      721 cgtcaatatg tattatacct gctttagcac caattttact tacaacccgg gtagagtttg
      781 cactggcgta agtcacagcc cattccaatg gttcgcctgc ggctatcatc gcagtaaatc
      841 cagagctaaa tgcatcaccg gcacctgttc tatctacaac ggggacgttt tcatacatgc
      901 ccgctttgac aatgtgccat ctatcggcag ccatagcacc tttgggccca tctgttacta
      961 caacataatg cacatcttta ttcgctgctc taactaattc ttctagcgtt tcccctttat
     1021 ataagctttg catttcttgc ttattcatag acaaaatagt gagcttagga attagttttt
     1081 taagctctgc tttttgagcg agttcttttt tgccagggtt aaaagcaatt tttatattat
     1141 gttgttgtgc atatgcaaat acggtatgta gtacttcaaa gttaccatct aagcttgtta
     1201 aatagatcca ttctggttta gtgtcatgaa aatctgctgt ttttatactg tagtgagcgc
     1261 tggcacccct ataggctaaa atagtccgct cgccacttgg g
//
LOCUS       DAOPLS010000230         1296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_125789_length_1296_cov_3.120870, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000230 DAOPLS010000000
VERSION     DAOPLS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125789_length_1296_cov_3.120870"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..353)
                     /locus_tag="PKV52_04360"
     CDS             complement(24..353)
                     /locus_tag="PKV52_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99476.1"
                     /translation="MSYYTRIFGTTMSVTPVYYELQNGEQFVYVYADTLVQPNADGVD
                     EEVVQLVRLGIDMVAANPRRFDKNNRSTQRLTLHFRDTLPPVPATVRPITQIPEREVL
                     EQVLGNE"
     gene            complement(383..>1296)
                     /locus_tag="PKV52_04365"
     CDS             complement(383..>1296)
                     /locus_tag="PKV52_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99477.1"
                     /translation="VVVDYTFLQLEGMPYEPLTMFMMNDSIALRLRVSAKITTPFSVR
                     VRYADLQQRLAKGRGQDLDIVITDTHLELVGKRIHHKIPLVANVRSFTTASADTPMTK
                     AAFRFTPLLGLEYIASNNGHILGFNADRTLVRFFTADSYTSNFVLTGVQGRVLTSVFP
                     SAEYAIIEGQLHVRTADNYGIFALVKMDIPNMDILLNVSPTVSIDLTKADAEEIQTLG
                     FSDTCTFIVDGDNLRILAQSFDAGEGEWVKTLPTIHPLTLVRVPTRTIRELVGDLTVN
                     ILSLPGTMALSLVTSDTITVTSGIVRA"
BASE COUNT          352 a          278 c          276 g          390 t
ORIGIN      
        1 caacagtatt ttggtactaa ttattattcg ttaccaagca cctgttctaa tacttcgcgt
       61 tcaggtattt gtgtgattgg gcgtactgtt gctggcacag gcggcaatgt gtccctaaaa
      121 tgtagtgtta accgctgtgt gctgcgattg tttttatcaa aacgacgtgg gtttgctgct
      181 accatatcaa taccaaggcg gactaactgc acaacttctt cgtctacacc atcggcgttg
      241 ggttgcacta aagtatctgc atagacataa acgaattgct ccccgttttg gagttcgtag
      301 taaacgggtg taaccgacat ggttgtgcca aaaatgcgtg tataataaga catatctgtg
      361 tacatatgtc ttattataca cattacgcac gtactatacc cgatgttaca gtaatagtgt
      421 cagatgtcac taaagataat gccattgtgc cgggcaacga tagtatattg acagtcagat
      481 caccaaccag ttcacgtata gtgcgggttg gtacacgtac caatgttaat ggatgtattg
      541 tgggtagcgt cttaacccat tcaccttcac ccgcatcaaa tgattgtgct aagatacgta
      601 agttgtcgcc atcaacaata aatgtgcaag tgtctgaaaa acccaacgtt tggatttcct
      661 cggcgtctgc ttttgttaga tcaatactaa ctgttggtga tacgttcaac aatatatcca
      721 tgtttggtat atccatcttc accaacgcaa aaataccata attatcggct gtacggacat
      781 gtaactgtcc ttcaataatg gcatattctg cactcggaaa tacacttgtt agcacacgcc
      841 cttgtacacc agttaataca aaattgctgg tgtaactgtc ggctgtaaaa aatcgcacta
      901 atgtacgatc tgcattaaac cctagtatgt ggccgttatt gctggcgatg tattctaatc
      961 caagcagggg tgtaaagcga aatgctgctt tcgtcattgg tgtatcggca ctggctgttg
     1021 taaagctacg cacatttgcc acaaggggta ttttgtggtg tatacgctta cccaccaatt
     1081 ccaggtgcgt atctgtaata acaatatcta agtcttgtcc acgccccttt gctaaacgtt
     1141 gctgtaaatc agcgtagcgt acacgcacac taaacggggt tgttattttg gcggatacac
     1201 gcagccgtag cgcaatgctg tcgttcatca taaacattgt tagcggttcg tagggcatac
     1261 cttcaagttg taaaaaggtg tagtctacaa ctactt
//
LOCUS       DAOPLS010000231         1292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_126428_length_1292_cov_2.084883, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000231 DAOPLS010000000
VERSION     DAOPLS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126428_length_1292_cov_2.084883"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKV52_04370"
     CDS             <1..509
                     /locus_tag="PKV52_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HPD99478.1"
                     /translation="CAKKEYVRQMPGRIVGRTEDSEGRVGYTLTLQAREQHIRRAKAT
                     SNICTNQGLMVTAATIYMTLMGAQGLRDVAATCHDNARELKERLTTIAGVHTVFGAPF
                     FNEFVIQVEKPIDDLLAQLSDFGIQGGYALESEYPELNNCLLVCATETKTAQDLERYQ
                     QLLARCLV"
     gene            521..>1292
                     /locus_tag="PKV52_04375"
     CDS             521..>1292
                     /locus_tag="PKV52_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPD99479.1"
                     /translation="MLSFEKSREGRVAGSQLSSARHDASDIPVELLRNDLPKLPQLSE
                     LQVVRHFTKLSRKNFSIDTNFYPLGSCTMKYNPRSAHKLASLPGFLGRHPYSDSYSSQ
                     GFLRCMYELQEMLIEVTGMKAVSLAPMAGAQGEFAGVKMIRAYHHARGEDSRNEILVP
                     DAAHGTNPASAFMCGYQVKEIPTTAEGDVDLSALKQMVGPQTAGIMLTNPSTFGVFER
                     QIKEIADIVHKAGGLLYYDGANMNAILGQYRPGDMGFDV"
BASE COUNT          374 a          222 c          305 g          391 t
ORIGIN      
        1 tatgtgctaa aaaagaatat gtcagacaaa tgcctggacg tattgttggt cgcacagaag
       61 atagtgaagg tcgtgttggt tataccttaa cgttgcaagc gcgtgagcag catatcagac
      121 gtgctaaagc cacttcaaat atttgtacga atcaaggatt gatggtaaca gcagcaacaa
      181 tctatatgac attgatgggt gctcaaggat tgcgtgatgt cgcggctact tgtcatgata
      241 atgcacgtga attaaaagaa cgtttgacga ctattgcagg tgttcatact gtttttggcg
      301 cacctttttt caatgagttt gtgatacaag ttgaaaagcc aatcgatgat ttattggcgc
      361 aattgagtga ttttggtatc caaggtggtt atgcattaga aagtgaatat cccgaattga
      421 ataactgtct tttggtttgt gcaacagaaa ctaagacggc acaagattta gaacgttatc
      481 aacaactact agcccgttgc ttagtttagg agagagcttg atgttaagtt ttgaaaaatc
      541 tcgagaaggt cgtgttgcag gtagtcaatt atcatcagca cgtcatgatg catcagatat
      601 tcctgtagaa ttgttacgaa atgacttgcc aaaattacct cagctatctg agcttcaagt
      661 tgtacgccat tttacaaagt tatctcgtaa aaacttttca attgacacga atttctatcc
      721 attaggttcg tgtaccatga aatataatcc acgttcagcg cataaactgg catcgctccc
      781 tggattttta gggcgtcatc cttacagtga ctcgtactca agccaaggct ttttgcgttg
      841 tatgtatgaa ttacaagaaa tgctgattga agtgacaggt atgaaagcgg tttcattggc
      901 gcctatggca ggagcgcaag gtgaatttgc aggagtcaaa atgattcgtg catatcatca
      961 tgcacgtggt gaagatagtc gaaatgaaat tttagtgcct gatgcggctc atggaactaa
     1021 tcctgcttca gcttttatgt gtggttatca ggtaaaagag attcctacaa cagcagaggg
     1081 tgatgtcgat ctttcagcgt taaagcaaat ggttggtccg caaacggcag gcatcatgtt
     1141 aacgaatcct tctacgtttg gtgtgtttga acgtcagata aaagaaattg cagatatcgt
     1201 acataaggcc ggcggcttac tttattatga tggtgctaat atgaatgcga tacttggtca
     1261 gtatcgacct ggtgatatgg gatttgatgt gt
//
LOCUS       DAOPLS010000232         1291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_126556_length_1291_cov_2.322816, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000232 DAOPLS010000000
VERSION     DAOPLS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126556_length_1291_cov_2.322816"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PKV52_04380"
     CDS             complement(<1..695)
                     /locus_tag="PKV52_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD99480.1"
                     /translation="MRLISLWIGKSVLLGLKLLNKRGAALPGLVVEKIYPNFLSKMLQ
                     TLPEGVVVISGTNGKTTTTKMLSAVLKGTGKKVLTNPTGSNFTRGVVAAIIADASWGG
                     RLRQDIAIIELDEAYASKFVEHISPRMSVILNVMRDQMDRFGEIDHTARLLQNVVSKT
                     TDVVILNRDDSRVYAMRNQTKSQSMFFGVSHDLRAIYRNDDELHGDISKTNTNATVEL
                     LGVNDDKIVVKIE"
     gene            complement(744..>1291)
                     /locus_tag="PKV52_04385"
     CDS             complement(744..>1291)
                     /locus_tag="PKV52_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99481.1"
                     /translation="SKIPITVGGSGLFINGFIYEYSFVAPNPEVRKTYQSWSVTQLQQ
                     EIIKRNIALPVNSKNKRHLTLALERANRPTPTKKALPKDTMIIGIAPEKDVLLGNIEQ
                     RAHQMIQEGVLEEVSHMYKTYNTSPALLGGVYRTVQPYLRGEQSIDATLQSIITYDKK
                     LAKRQLTWFKRNPDITWFTSP"
BASE COUNT          333 a          283 c          236 g          439 t
ORIGIN      
        1 tcttcgattt taacaacaat tttgtcatca ttcaccccta atagttctac tgtggcgtta
       61 gtattagttt tacttatgtc accatgtaat tcgtcatcat ttctataaat tgcccgtaag
      121 tcatgagaca caccaaaaaa catactctga gactttgttt gatttcgcat tgcataaaca
      181 cgactgtcgt ctcggtttaa aatcacgaca tctgtagttt tacttactac gttttgtaag
      241 agccgagctg tgtgatctat ctcgccaaaa cgatccattt gatcgcgcat gacatttaat
      301 attacactca tccgtggtga aatatgttca acaaactttg atgcatacgc ttcgtcgagc
      361 tctataattg ctatgtcctg ccgcagccta ccaccccatg aagcatcggc aataatcgcc
      421 gccacgacgc ctctcgtaaa attgctgcct gttgggtttg tgagcacttt tttaccagtg
      481 ccttttagga ctgcagataa catttttgtt gtggtagttt taccattggt gccactaata
      541 acgacaaccc cttctggtaa cgtctgcaac attttagaca aaaagttagg atatattttt
      601 tctactacga gtccaggcaa ggctgcacct cttttattga gtagcttaag accaagcaga
      661 acactcttac caatccataa actaattaat cgcatacttt acagtgtacc ttgcttatgg
      721 ttcatgaacc actctaatgc ttcttaaggg ctggtgaacc aagtaatatc tgggttgcgt
      781 ttaaaccacg ttaattgtcg cttggctagc tttttgtcat acgtaatgat tgattgaaga
      841 gtagcgtcta ttgattgttc gccccttaag taaggctgga cagtacgata cactcccccc
      901 aataaagctg gtgacgtatt atatgtttta tacatgtgac taacttcttc tagcacccct
      961 tcttgtatca tttggtgagc cctttgctct atattaccaa gcaatacgtc tttttctggg
     1021 gcaataccaa taatcattgt gtctttaggc aaagcttttt tagtgggagt aggtctatta
     1081 gctcgttcta gtgcaagcgt taagtggcgt ttgtttttgc tatttactgg tagtgcgatg
     1141 ttcctcttta taatttcttg ctgtaactgt gtaacactcc atgactgata ggtttttcgt
     1201 acctcaggat ttggggccac aaacgagtac tcatatataa agccgttaat aaacaatcca
     1261 cttccaccga ctgttattgg tatcttgcta c
//
LOCUS       DAOPLS010000233         1289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_126882_length_1289_cov_1.957050, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000233 DAOPLS010000000
VERSION     DAOPLS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126882_length_1289_cov_1.957050"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKV52_04390"
     CDS             <1..535
                     /locus_tag="PKV52_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99482.1"
                     /translation="SRITWATFAESQRGEADPEHFELGGLSVFYTTVHISDEQRPFPY
                     AVAAAVPPNQEAYTICVSDDVPESLRGLWAWHELHDFSEVGHDHPKRCVSSEIVVAAE
                     MDPESDEYQEYIKYRIHFYMHLEKFMAEDIAAKGEVSEYDPYDVEGCQKAIQFLRGLR
                     IKNQEQCLIATHFRSSD"
     gene            complement(503..>1289)
                     /locus_tag="PKV52_04395"
     CDS             complement(503..>1289)
                     /locus_tag="PKV52_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPD99483.1"
                     /translation="FSLHSVLFAVLFGTLFALVYRGEKILVDIKKTSKRAWIAIIMFG
                     LFASGLFIVFRFLGLAQSSGTFATLGQVITTSLTAILAFIILRERLAKQFWLFFVLII
                     LATYVVSTNSVTLSTPKVGDLFILLAAFFLAIANIFAKIAIPKTTPIVLSVGRFCTGA
                     IFFILATMLLFGAEALFSEFSYWSILSGFFWTVNILAFSFAISRIGITLATSVLMFAP
                     IITMILEITLLKQHFSLLQIVAGLVVVSSGVMLLITASKVSRN"
BASE COUNT          430 a          241 c          284 g          334 t
ORIGIN      
        1 atcgagaatc acttgggcta cctttgctga aagtcagcgg ggcgaagccg atcccgaaca
       61 tttcgagtta ggcggtctca gtgtgtttta tacgactgtc catatttctg atgagcagag
      121 accatttcca tatgctgtgg ctgcggcagt accgccaaat caagaggcct acacgatatg
      181 cgtgagtgac gatgtgccag aaagcctacg cgggttgtgg gcgtggcatg agctgcatga
      241 ttttagcgaa gttgggcatg atcatcctaa gcgatgtgtg agttctgaga ttgtcgttgc
      301 tgcagagatg gacccggaat ctgatgaata tcaagaatat ataaaatatc gtatacattt
      361 ctatatgcat cttgaaaaat ttatggcaga agatatagca gcaaaaggcg aggtgtcaga
      421 gtacgatcca tatgatgttg aggggtgtca aaaggctatc caatttctgc gcggcctacg
      481 tatcaaaaac caagaacagt gtctaattgc gactcacttt agaagcagtg attaacaaca
      541 taacgccact actgaccact accaatccag caactatctg tagcagacta aagtgttgtt
      601 ttagtaaagt tatctcaagt atcatcgtaa taataggtgc aaacattaga acagaagtag
      661 caagtgtaat ccctatacga gaaattgcaa aactaaaagc caggatgttt acagtccaga
      721 agaatccact taaaattgac caatagctaa actctgagaa tagagcttct gcaccaaaaa
      781 gcagcatggt tgccaaaatg aagaatatgg cgccagtaca aaatcttccc acggaaagta
      841 caatcggtgt tgtctttgga attgctattt ttgcaaaaat atttgctata gcgagaaaaa
      901 atgctgctag taatataaat aggtcaccta ctttaggagt tgatagtgtg acgctgtttg
      961 tagaaacaac gtatgttgcc agaatgatta gtacaaagaa taaccagaat tgttttgcta
     1021 gtcgttctct gagtatgata aaagcaagaa ttgctgtaag tgatgttgtg attacttgtc
     1081 caagtgttgc gaaagtacca ctagattgag cgagtccaag aaatcgaaaa acaataaata
     1141 agccggaagc aaaaagccca aacataataa ttgcaatcca tgcacgtttg gatgttttct
     1201 taatatcaac aagaattttc tctccacggt ataccagcgc aaataaagtt ccaaacaaca
     1261 cagcaaacag gacactgtgc agtgaaaag
//
LOCUS       DAOPLS010000234         1286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_127338_length_1286_cov_1.700244, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000234 DAOPLS010000000
VERSION     DAOPLS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127338_length_1286_cov_1.700244"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKV52_04400"
     CDS             <1..216
                     /locus_tag="PKV52_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99484.1"
                     /translation="DEEPFTNDSMTSVKGGGMYVYIEVEDVDQEYQRQTLLGLTPATK
                     PRDWDWGNREFILKDPDGYKLVFFAKK"
     gene            280..>1286
                     /locus_tag="PKV52_04405"
     CDS             280..>1286
                     /locus_tag="PKV52_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99485.1"
                     /translation="MQKLVKAISRGKKHVPFFVLCVLLLAALVISALTTTKQIGVNAV
                     PSISYEQSVPSTGSRIFNGNGIALDSNDNMFVINRSNPTDIKKINATGDLVTQWGSYG
                     SGNGQFSLPHIVAVDNSDNVYVTDENFYVQKFDNNGNFVYKIGGYGTGDGQFSSLIYL
                     AFDSSNNMYATGSNGTIQKFDISGNFVSRIGSAGTGDGQFNSSGPYGVAIDSNDNIFA
                     VDSSRIQKFDSSGTFVTKWGSSGCCGSGFFSSPRYIAIDSSDNVYVGQISGQYMVQKF
                     DNDGVYISEVGEPTIFGIGGMAFDSSDRLNLITEPTSGTSYAQDIIQLDTSLATVGRI
                     A"
BASE COUNT          369 a          261 c          320 g          336 t
ORIGIN      
        1 gatgaggagc cattcactaa cgattcaatg acgagtgtca agggcggggg catgtacgtc
       61 tacatcgagg ttgaggatgt cgaccaagag tatcaacgac aaactttact tggtctgacg
      121 ccggcaacta agcccaggga ctgggattgg ggcaatcgtg agtttatact caaagacccc
      181 gacggctaca agttagtgtt ttttgcaaaa aagtaacgca ttacggttgt gcttacctct
      241 gttcttttga tatgatggta tcaaaggcag taaacaaaaa tgcaaaaatt ggtaaaagct
      301 atttctagag gtaaaaaaca cgtacccttt tttgtgctgt gtgtactatt gttggctgcc
      361 ttggttatct ctgcgctgac aactacaaaa cagattggcg ttaatgcagt gccaagtatt
      421 agctacgaac agagcgtgcc gtccactggt tcacggatat tcaatggcaa cggtatcgca
      481 ctcgactcta atgacaacat gtttgtcatc aatagatcga atccgacaga tataaaaaag
      541 atcaacgcaa caggtgatct tgtcacacag tggggctctt acggcagtgg taatggccag
      601 ttttcgcttc cgcatatagt ggcagtcgac aatagtgata acgtgtatgt caccgacgag
      661 aacttttacg ttcaaaagtt tgataacaac ggcaactttg tgtataagat cggtggctat
      721 ggtacgggcg atggccaatt ctcgagccta atatacctcg catttgatag cagcaacaat
      781 atgtatgcaa cggggagtaa tgggactata caaaagtttg atatatccgg aaactttgtc
      841 tcaagaattg gttcggcagg tacgggcgat ggccagttca atagctcggg cccatacggg
      901 gttgcaatcg actcgaacga taacattttt gcggtagaca gtagtcgcat ccagaaattt
      961 gatagtagtg gcacatttgt tacaaagtgg ggatcaagtg gctgctgcgg atcaggtttt
     1021 ttcagctcac cacgatacat cgccatagac tccagcgata acgtatacgt gggccaaatc
     1081 tcgggtcagt acatggtgca aaaatttgat aacgacggcg tatacatttc ggaagttggc
     1141 gagccaacaa tttttggtat tgggggcatg gcgtttgata gctctgacag acttaacctc
     1201 ataactgaac caacaagcgg taccagttat gcgcaagata tcattcagtt agatacgagt
     1261 cttgcaacag tcggcaggat tgccag
//
LOCUS       DAOPLS010000235         1280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_128120_length_1280_cov_4.128980, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000235 DAOPLS010000000
VERSION     DAOPLS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128120_length_1280_cov_4.128980"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKV52_04410"
     CDS             <1..741
                     /locus_tag="PKV52_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HPD99486.1"
                     /translation="LDKVTARGKLSPKHHLQSLLFGLSMGMIVVAVVMLSFFNERFIA
                     PFITPSRTASASPIIINPNEQVGPESKVIIPKINVDVPVVYDMTTNEEKAVQAALENG
                     VVHYPSTPVPGQNGNVVIVGHSSNNLLNRGKYKFAFVLLNKLQEGDTITMQYGGKRYV
                     YKVYEKVIVKPSDVGVLGPTDKTASLSLITCDPPGTSINRLVVRAEQISPNPNGNVAA
                     STANQVANTPDIVPGNAPSLFERLFGWL"
     gene            complement(748..>1280)
                     /locus_tag="PKV52_04415"
     CDS             complement(748..>1280)
                     /locus_tag="PKV52_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99487.1"
                     /translation="LQPIAASQTILLDIARYEGDWFFVVGSNAQENTHIYINPLQNAD
                     NIDTTGALRFQLKLPLPAARYVGFSDNARFIGVQNDTAFTVYDAELQTIYRFDSPVAL
                     PPEGAQWLDGHRFHVVSGGSEHVFEFDGANFQTLTEAVNGQKAFYDNDFVRLFTLAPQ
                     ANNSLNFQVGFLTLTN"
BASE COUNT          385 a          268 c          287 g          340 t
ORIGIN      
        1 ctagataagg tgactgcccg cggcaagcta agccctaaac accatttaca gtcgcttttg
       61 tttggtttga gcatgggtat gattgtagta gccgtcgtca tgctcagctt ttttaatgag
      121 cgatttattg cgccctttat tacaccaagc cgcacggcga gcgcatcgcc aataatcata
      181 aatccaaatg agcaagtcgg gccagaatca aaagttatta ttcctaaaat caatgttgat
      241 gtaccagttg tctatgacat gactaccaat gaagaaaaag ctgttcaagc ggccctggaa
      301 aacggagtcg tgcactatcc gagcactcct gtgccagggc agaatggtaa tgtcgtgata
      361 gtcgggcact catcgaataa tctactcaac agaggtaagt acaagtttgc atttgtacta
      421 ctaaacaaac tgcaagaagg cgacaccata acgatgcaat atggcggcaa gcgctatgtg
      481 tataaggtat acgagaaagt catcgttaaa ccgagtgatg ttggtgtgct tgggccaaca
      541 gataaaacgg cttctttaag cctgattacg tgtgacccac ctggtacaag tattaatagg
      601 ctggttgtgc gcgccgagca aatatcgccg aaccctaatg gcaacgtagc tgctagcacg
      661 gctaaccaag tagcaaatac gcccgatatc gtacccggaa acgcaccaag tttgttcgag
      721 cgtttgtttg gttggttata agataattta gttagtaagc gttaagaaac ctacttggaa
      781 atttaacgaa ttattagctt gtggagcgag ggtaaaaagg cgaacaaagt cattatcgta
      841 aaacgctttt tgaccgttca ctgcttctgt taatgtttga aaattagcac catcaaattc
      901 aaatacatgc tcgctaccac cactcaccac atggaaccga tggccgtcta accattgcgc
      961 gccttctggt ggtaaagcaa ctgggctatc aaagcgataa attgtttgta gctcagcatc
     1021 atataccgta aaagctgtgt cattttgcac acctataaaa cgagcattat cagaaaagcc
     1081 aacatagcgt gctgcaggta agggtagttt taattgaaaa cgaagagcgc cagtcgtgtc
     1141 tatattatct gcattctgca gtgggttaat ataaatgtgt gtattttctt gagcattaga
     1201 gccaaccaca aaaaaccaat cgccttcgta gcgagctatg tctaataaaa ttgtttggct
     1261 tgcggcaata ggttgtaata
//
LOCUS       DAOPLS010000236         1275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_128920_length_1275_cov_2.659836, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000236 DAOPLS010000000
VERSION     DAOPLS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128920_length_1275_cov_2.659836"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PKV52_04420"
     CDS             complement(<1..828)
                     /locus_tag="PKV52_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99488.1"
                     /translation="MAIAQTRTWLPLDTWAKIMGYNPWTFNGFAQFPNTLGEPCSDVW
                     IQEPGQQATMSREELAIAIREAENMVSEFVGYKLLPDWDSDLVIPPRFNTPPYFSSAT
                     TYSRPKSVRVKEKYLIQLGVERRHFLQNVAIVRVDLDNDGFAETGRITIAEATLDPNQ
                     VRIFYPDQDGDEMYEIRPVKSKGGGIFEFPMYLVPQLDLIMTPIVADPLDVADPNNFL
                     ATVDVYQVYNDTTTPVTLLYNGGDCATCEETSTAICGTIENEKLGYVNYITYTDPDKI
                     "
     gene            916..>1275
                     /locus_tag="PKV52_04425"
     CDS             916..>1275
                     /locus_tag="PKV52_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99489.1"
                     /translation="MYYLLKITLTKVAKRYITYEFDKYDTVLSASCYHENYQTGLFVY
                     DNEVLTTLNQKPNLRNVLHSLYDVSFEQLTFSTIDISDYILDDLADILIEVLPLWDKR
                     DEPIIILMGLDEETDCET"
BASE COUNT          383 a          235 c          241 g          416 t
ORIGIN      
        1 tattttgtca ggatcggtat atgttatgta gttaacataa cctaattttt cgttttcgat
       61 tgtgccacat atagcagtgc ttgtttcttc acaagtggca caatccccac cgttgtaaag
      121 caatgtgaca ggtgttgttg tatcgttata cacctgatag acatcaaccg ttgctagaaa
      181 attgttagga tcagccacat caagtgggtc tgccacaata ggtgtcataa ttaggtctag
      241 ctgtggcaca agatacattg ggaattcaaa tatacctcca cctttgcttt ttactggccg
      301 aatttcatac atttcgtccc catcttgatc ggggtaaaaa atacgtactt gatttgggtc
      361 tagtgtcgct tcggcaattg ttatacgccc tgtttctgca aagccatcat tatccaaatc
      421 aacacgtaca atagcaacat tttgcaaaaa atggcgtcgc tcaacaccta actgtattag
      481 gtatttttcc tttacgcgca ccgactttgg ccgactatat gttgtagcag aactaaaata
      541 tggtggggta ttaaaacgtg gtggtataac taaatcgcta tcccaatcgg gcaataattt
      601 atacccaaca aactcactaa ccatattttc agcttctcga atagcaattg caagttcttc
      661 tcgactcatt gtagcttgct gcccaggttc ctgaatccaa acatcactgc atggttcgcc
      721 aagtgtgttg ggaaactgtg caaaaccatt gaaagtccag gggttgtacc ccattatttt
      781 tgcccatgta tcaagtggta gccatgtccg tgtttgtgca attgccataa ctaaacaata
      841 aaagacagtt tagaagttgc aagttggtat caaagtaatg tttacaaaat tttgtaaagg
      901 gtttataata agattatgta ttatttattg aaaattactc tcacaaaagt agccaaacgg
      961 tatataacat atgaatttga taagtatgat actgttttgt cagcctcatg ttatcatgaa
     1021 aattaccaaa cgggtttatt tgtgtatgat aatgaggtgt taactacctt aaatcagaaa
     1081 cctaatctga gaaatgtcct tcatagtctt tatgatgtta gttttgaaca gctaaccttt
     1141 tcaacaattg atatttcaga ttatattttg gatgatctag cagatatact cattgaggta
     1201 ttacctttgt gggataaacg tgacgaaccg attataattt tgatggggct agatgaagaa
     1261 acggattgtg aaact
//
LOCUS       DAOPLS010000237         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_130392_length_1265_cov_5.074380, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000237 DAOPLS010000000
VERSION     DAOPLS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130392_length_1265_cov_5.074380"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..506
                     /locus_tag="PKV52_04430"
     CDS             99..506
                     /locus_tag="PKV52_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPD99490.1"
                     /translation="MAKASGKNVVTAVVVGAVAGYVTGILTAPKSGKETRKDIKDKAD
                     KYAAEAGKRLQAARDELNVVIEEATQKAKYYSDKGKKEVHELVGKAKVAQTKAKDVLQ
                     AVKQGEAQDKDLHKALTEASEAKQHLIDYLKKS"
     gene            640..>1265
                     /locus_tag="PKV52_04435"
     CDS             640..>1265
                     /locus_tag="PKV52_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPD99491.1"
                     /translation="MHNHTQFSLLDGLTQLGPLLDFAKEQGMEAIAKTDHGTLSGSVE
                     FYKEAKAKGIKPIIGIETYVAMRKHTDKDPAKDKQRYHLVLLAMNNTGYKNIMRLSTI
                     ANLEGFYYHPRIDHDLLEKYNEGVIALSACMSGEVANALKNGQRKQAKEVALWYKKTF
                     GDRYYLEIQDHGHPDNPLTNKEQEDINTKILDLAKELDIPVVVTCDAH"
BASE COUNT          451 a          226 c          280 g          308 t
ORIGIN      
        1 tttctgataa gataagacag tttgcactgc ctactgctgt catgtctatg gttacgaaag
       61 tcgtttcaca atataagcaa tcgaaagggg actaagtcat ggcaaaagca agtggcaaaa
      121 acgtagttac tgctgtcgta gttggagcag ttgcagggta cgtaacgggt atactcaccg
      181 ctcctaaaag tggtaaagaa acacgcaagg acattaaaga taaagccgat aagtacgcgg
      241 cagaagctgg caagcgatta caagcagccc gtgatgaact taatgtagta atagaagaag
      301 ctacccaaaa agcaaaatat tattctgaca agggtaaaaa agaagttcat gaactagtag
      361 ggaaagcaaa agtcgcacag acgaaagcta aagatgtctt gcaggctgtt aaacaaggcg
      421 aagcgcaaga taaagatttg cacaaggctc tcaccgaagc gagcgaagca aagcaacatc
      481 tcatagatta tttaaaaaaa tcttagataa gactgtagtg taatttacta gggtccgtga
      541 catattgcac gggccctttt ataataatta gagataaatc attacataac ggtgaggggt
      601 gctgttatcg ctacgcttcg tcctagtgac tatgtgcatt tgcacaatca tacgcaattt
      661 agtttattag atggtcttac acaattagga ccattgcttg attttgcaaa agaacagggt
      721 atggaagcga tagctaaaac agatcatggc actttaagtg gatcggtaga attttacaag
      781 gaagccaaag caaagggtat aaaaccaatc ataggcatag agacatacgt tgctatgcgc
      841 aaacacactg acaaagaccc agcaaaagat aaacagcgat atcatttggt gctgctcgcc
      901 atgaacaaca ctgggtataa aaatataatg cggctttcta ctattgcgaa cttagaaggc
      961 ttttactatc atcctcggat agatcatgat ttgttagaaa agtataatga aggtgtaatc
     1021 gccttgagtg cgtgtatgag tggtgaagta gccaatgccc taaaaaatgg gcaacgtaag
     1081 caggcaaaag aagtagcatt gtggtataaa aaaacttttg gtgatagata ttatttagaa
     1141 attcaagacc acggccaccc tgataatccc ctcaccaaca aagaacaaga ggatattaat
     1201 acaaaaattc ttgatcttgc caaagagcta gacataccag tcgttgtaac ttgtgacgcg
     1261 catta
//
LOCUS       DAOPLS010000238         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_132320_length_1253_cov_3.515025, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000238 DAOPLS010000000
VERSION     DAOPLS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132320_length_1253_cov_3.515025"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..472)
                     /locus_tag="PKV52_04440"
     CDS             complement(32..472)
                     /locus_tag="PKV52_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99492.1"
                     /translation="MKQKVQTIIQKLSAIDARTLGLLAVLLVAVSVTYSSAKIIHKNY
                     QLEQQITILQQKNALQEQINSNQKLKNEYYKTDTYLELAARKFFNKAAAGEILVLVPE
                     DVAMSYTIPPQADNAAKSNTTPSFLTNWRMWLDFLSGKPIDKES"
     gene            615..690
                     /locus_tag="PKV52_04445"
     tRNA            615..690
                     /locus_tag="PKV52_04445"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Ile,seq:cat)
     gene            complement(1005..>1253)
                     /locus_tag="PKV52_04450"
     CDS             complement(1005..>1253)
                     /locus_tag="PKV52_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPD99493.1"
                     /translation="ASVIKDRESGRSKGFGFVEYETPEEGKAAIDALNNSELDGRTIV
                     VNEARPKEDRPRNGGGGSRGGYGGGNRSGGGSFRTSSW"
BASE COUNT          315 a          279 c          269 g          390 t
ORIGIN      
        1 taacgtattt taaggcttta actgcaatgt gtcaagattc tttgtctatt ggcttgccac
       61 tcaaaaaatc tagccacata cgccaattgg taagaaacga cggtgttgta ttagattttg
      121 ctgcattatc tgcttggggc ggaatagtat aagacatcgc aacatcttct ggtacaagta
      181 caagaatttc gcccgcagca gctttgttaa aaaacttacg tgcagctagt tctaggtacg
      241 tatctgtttt gtaatactca ttttttagtt tttggttgct attaatttgt tcttgcaagg
      301 catttttttg ttgcaaaatg gtgatttgtt gctctagctg gtagtttttg tggataatct
      361 tagcactact atatgtaaca gatacggcta ctaagagcac tgccagcaaa cctaatgttc
      421 gggcgtctat tgcgctgagt ttttgtataa ttgtttgtac tttttgcttc atggtgtgta
      481 atctaattat atcgtatctg taaaatcatt gtgctgtttg gtatactata gcagctaacc
      541 attgttttgg cagctcgcac atatatacca cagggtgcag tccgtcaaca gacgggcaga
      601 cacctttaca atacgggggt gtagctcagt ggttagagca gtcggctcat aaccgattgg
      661 tcacaggttc aaatcctgtc acccccacca aaagtgctcc catagtgggg agtattttta
      721 tttttagatt ggtcactcgg ctgttccagc ctcaacacgt gtgctagttg gcggcattat
      781 aagcaaacaa ttagcgacgc ggaaatcaag cgtcaatgag cgtgaactca cactgccact
      841 ggcagtcttc gggttcaaat cctgtcaccc ccaccaaata gagttaaatt atccgccaga
      901 aggcggattt tttatgcaat atcttccacg agcaaggttg taaaatctga taactaaaat
      961 agctcctatt gctaggagct attttagtta tcagcgtggt aagattacca gctgctggtg
     1021 cggaatgaac cgccgccact tcgattacca ccaccataac cgccacggct tccgccgcca
     1081 ccgttacgag gacgatcttc ttttgggcgt gcttcattta ccacaatggt acgaccatct
     1141 agctctgagt tgttcagagc atcgatagca gctttacctt cttctggagt ttcgtactcc
     1201 acgaagccaa agcctttgct gcggccactt tcgcgatctt tgatcacgct agc
//
LOCUS       DAOPLS010000239         1245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_133656_length_1245_cov_2.074790, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000239 DAOPLS010000000
VERSION     DAOPLS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133656_length_1245_cov_2.074790"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..672)
                     /locus_tag="PKV52_04455"
     CDS             complement(289..672)
                     /locus_tag="PKV52_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99494.1"
                     /translation="MVFQGGSAIPVYNIDASQLVENGGDFRVIGYLAYAIAPPSGTDF
                     TNVNQIQFNTSYIPTSQVAGTIYWNEAEKTLNIVVDDNVVLQAGQEILLPVRNQSGVD
                     IGDGAPVMYAGTTGNSGRTLIQPVL"
     gene            complement(687..827)
                     /locus_tag="PKV52_04460"
     CDS             complement(687..827)
                     /locus_tag="PKV52_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99495.1"
                     /translation="MGNFKRFKFLWFEFGFEWWGDLRPENVTLVDGTVFKPVGLGFWR
                     KQ"
     gene            complement(866..1039)
                     /locus_tag="PKV52_04465"
     CDS             complement(866..1039)
                     /locus_tag="PKV52_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99496.1"
                     /translation="MTFDELSEIAKQYDTLVSVVAYEIAKKEPNLPRLEYRRRAEQLL
                     LQATVVKVLRDSK"
     gene            complement(1036..>1245)
                     /locus_tag="PKV52_04470"
     CDS             complement(1036..>1245)
                     /locus_tag="PKV52_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99497.1"
                     /translation="KAWEQIERFFDLYSQFETNFNWWQANKMHMRRCHRVTTCDAHEE
                     VPRRENVAKAIANGRHQLDWIKSDK"
BASE COUNT          364 a          280 c          223 g          378 t
ORIGIN      
        1 aacgcactgt taacacccca ttaacggcag aattcataac agcagcaatt tgaatacgat
       61 agttaggtgt atcgggtggt gttttagtta aataacctgc cgttgtagcc gacacataaa
      121 gtatatcacc atcgatccaa acttcgccat aaggtacgcc ggtagtgtta atttcacgta
      181 ctttaccaaa ccatgttacg aaaccatctg agttgttggg gattggctct gtagcaagcc
      241 ctaaaataaa acttgggtca atactaccat cggcgatagc gggttgaatt aaagtacggg
      301 ttgaattaaa gtacggcctg aattgccagt tgtgccagca tacataactg gcgcaccatc
      361 accaatatca acaccgcttt gattacgcac cggtaataaa atttcctgtc ccgcctgtaa
      421 aacaacgtta tcatcaacaa caatgttgag tgtcttttca gcttcgttcc agtaaattgt
      481 acccgcaact tggctagttg gtatgtaact ggtattaaat tggatttgat ttacgtttgt
      541 aaaatcagtg ccgcttgggg gtgctatagc atacgctaaa tagccaataa ctctaaagtc
      601 gccaccgttt tcaaccagtt gggaagcatc tatattgtag acagggattg cactaccgcc
      661 ttgaaatacc atgtctacat tatagattac tgtttacgcc aaaaccctag ccctactggc
      721 ttaaagactg tgccatcaac taaagttaca ttctcagggc gtaagtcgcc ccaccattca
      781 aaaccaaatt caaaccacaa aaacttaaat cgcttaaagt tacccattag ctcattatat
      841 catatactat ctactgattg attcgttatt tgctatccct taacactttt acaacagtcg
      901 cctgtaatag caattgttca gcacgcctac ggtattctaa tcgtggcaga ttgggttcct
      961 tttttgctat ttcataagca acaaccgaaa ccaacgtatc atattgtttt gcaatttctg
     1021 atagttcgtc aaatgtcatt tgtcactctt tatccaatct aattgatgtc tgccgtttgc
     1081 aattgcttta gcaacgtttt cacggcgagg gacttcttcg tgtgcatcac aagttgtaac
     1141 acgatgacac cggcgcatat gcattttatt ggcttgccac caattaaaat tagtttcaaa
     1201 ttgactataa agatcaaaaa accgttcgat ttgttcccat gcttt
//
LOCUS       DAOPLS010000240         1243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_133864_length_1243_cov_3.524411, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000240 DAOPLS010000000
VERSION     DAOPLS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133864_length_1243_cov_3.524411"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKV52_04475"
     CDS             complement(<1..1060)
                     /locus_tag="PKV52_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPD99498.1"
                     /translation="MAKRKTRRVTAKKRSKKNSAPEHVLPVGFWQQASAVGLIALSIL
                     MIVAWFGVGGPVLEWLQNAFMASIGFGTYIVPVVAVYVAVEIFRSEDNRLPFVMKFAS
                     FLVIAWVSGLFGLIEKDDKTSYGGFVGDTINSGILSLVDKGVAIFLYSLLIIITVLFI
                     LRVSPMAIIEKIKDLLATAEREEQESNSNIMKKATKASKSTDIGELKLNAGVPMMQAE
                     EEPKKLHLSSLKNSVVQDKQAEEKSALVAISDPNWKAPSLDLLEKKQKPADPGDVQHN
                     AQAIKDTLHEFNIDVEMEGANIGPKVTQYTLKPPAGVKLTKISQLETNIALNLAAQSL
                     RIEAPIPGKRAVGIEVPNR"
BASE COUNT          357 a          281 c          202 g          403 t
ORIGIN      
        1 ttctatttgg aacctctatg ccaactgctc gcttacctgg tattggagcc tcaatacgaa
       61 gactttgagc agcaaggttt aaggcaatat ttgtttcaag ttgagatatt tttgttaact
      121 tcacgccagc tggtggcttt aacgtatact gcgtaacttt tggcccaata ttcgcacctt
      181 ccatttcgac atcaatgtta aactcatgca gtgtatcttt tatcgcctgt gcattatgtt
      241 gtacgtcacc agggtctgct ggcttttgtt tcttctctaa cagatcaaga ctaggggctt
      301 tccagtttgg atcactgata gctacaagcg cagatttttc ctcggcctgc ttatcttgaa
      361 ctacgctgtt ttttagacta gataaatgta gcttttttgg ttcttcttca gcttgcatca
      421 tcggtacgcc agcattcaat ttcaattcac caatatcagt ggattttgaa gcctttgtag
      481 cctttttcat aatattgcta ttactctctt gttcttcacg ctcagcagta gcaagtaaat
      541 ctttgatctt ttctattatc gccattggtg acactcgtaa tataaataaa actgtaataa
      601 taatgagtag actatataaa aatattgcaa cccctttatc tacaagagag agtatgccgc
      661 tatttatggt atcaccgacg aatccaccgt aagatgtttt atcgtccttc tctatcaagc
      721 cgaataaccc actaacccaa gcaattacta gaaagcttgc aaatttcata acaaacggta
      781 gacgattatc ttctgatcta aatatctcaa ctgccacata aacggcgact accggaacaa
      841 tgtaagtacc aaaccctata gatgccataa aagcattttg tagccactcc agcacagggc
      901 cgccgacacc aaaccacgca acaatcatca gaatagacaa tgcaatcaat ccaactgcac
      961 tagcctgctg ccaaaaacct actggtaaaa cgtgttcagg ggcagaattt tttttagaac
     1021 gctttttagc tgtaactcgt cttgtttttc gcttagccat aattacttta tattgtaaca
     1081 ctttttatat tgaatacctt agtcattaca ctgttaagat tgagtctctt gttcgtctaa
     1141 tacaactaac tcgtaggccg gccttcgtct gtaccataag attaaactaa tcattatcag
     1201 gtaaaaactc atccatgcta aatttacacc gactaatata gca
//
LOCUS       DAOPLS010000241         1242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_134025_length_1242_cov_3.740522, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000241 DAOPLS010000000
VERSION     DAOPLS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134025_length_1242_cov_3.740522"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1038
                     /gene="trxB"
                     /locus_tag="PKV52_04480"
     CDS             118..1038
                     /gene="trxB"
                     /locus_tag="PKV52_04480"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPD99499.1"
                     /translation="MQDILMIGAGPAALTAAVYTTREDIDTVLLEKAVIGGLAATTDW
                     IDNYPGFPEGISGLDLAENLRKQAERFGAKIELDEVSNIRKEGDVVVLDTFSGERRAK
                     AVLIATGTEYKKLGIPGEAEYLSRGVHYCATCDGAFYRDKNIIVVGGGNSAVQEALFL
                     TKFASHIDLLVRSDVFRASDVLLHELEKNKQITVHMNTTTDEIIGDGSKVTAVKGTDK
                     KANKQVTFQTDGVFVFVGLKPNTQFLGKDIELDEIGFVKTNDRLETSLPGVFAAGDVR
                     SGATMQIATAVGEGATAALRIREYLETLKK"
BASE COUNT          386 a          234 c          307 g          315 t
ORIGIN      
        1 ataagtggtc agatggcggt accagtgacc gtaataacta aacaggatga cacgcaagaa
       61 gttgtggttg gttataatat tgccaaacta gccccagctg ttgcgtagag gtaacacatg
      121 caagacatac tcatgattgg ggccggccca gctgctttaa ctgcagctgt atacacgaca
      181 cgcgaagata ttgacacagt attattagaa aaagcggtta taggtgggct cgccgcaacg
      241 acagactgga tagacaacta tccggggttt ccagaaggca taagtgggtt agatttagcc
      301 gaaaatttac gtaaacaagc cgagcgtttt ggtgcaaaaa tagaactaga cgaggtcagc
      361 aacatacgca aagaaggtga tgtagtggta ctcgatacat ttagtggtga gcggcgcgcc
      421 aaggcagtct tgatagccac tgggaccgag tacaaaaaac ttggtatacc tggtgaggct
      481 gagtacttgt cgcggggtgt tcattattgt gcaacatgtg atggcgcttt ttatcgcgac
      541 aaaaatatta ttgttgttgg cggtggtaat tctgccgtac aagaagcact gtttttgact
      601 aaatttgcga gccatataga tctattggtc cgcagcgatg tctttagagc atcagatgtg
      661 ctgctgcacg aactagagaa aaataagcag attaccgttc acatgaacac aactacagat
      721 gaaattattg gtgatggcag caaagtaaca gccgttaaag gcaccgataa aaaagcaaat
      781 aaacaagtga catttcagac agatggtgta tttgtatttg taggcttaaa gccgaatact
      841 caatttttag gcaaagacat cgagcttgat gagattggtt ttgttaagac aaatgaccgt
      901 ttagaaacgt cacttcctgg tgtatttgcg gcaggggacg tacggagtgg ggctaccatg
      961 cagatagcta ctgccgtcgg agaaggcgca acagcggcgc tgcgtattcg cgaataccta
     1021 gaaacgctta agaaataatc gttcagccca gtaactagtt gcaatattta cttaataatg
     1081 ttaaataata tagtagtgcc ttatagacaa atcactactt accaaacagt tattgctcaa
     1141 gctcgggcgt atcgcaaagt acgtgccttt atggaatgta cactcaaaaa gtacaccatt
     1201 accattacag agtggcttat tataggtacg gcgatagatg ca
//
LOCUS       DAOPLS010000242         1238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_134755_length_1238_cov_2.491970, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000242 DAOPLS010000000
VERSION     DAOPLS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134755_length_1238_cov_2.491970"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(726..1163)
                     /locus_tag="PKV52_04485"
     CDS             complement(726..1163)
                     /locus_tag="PKV52_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99500.1"
                     /translation="MKSLTVTDDVVIAGNLTVQNVTVANLTVNGHIITAGNTPVATPG
                     TAAGTEDTLNNISAPQVTIEGNDTAGTITIVAGANTTAGTLAEVTFNQAFGNTPKVVI
                     TGQDIDSASIQVFATPTANGFTFGTPNTTQTSKTYKYSYFVIE"
BASE COUNT          323 a          212 c          321 g          382 t
ORIGIN      
        1 gcaccagggc gccggtgttg gtgaggatac gggcttggaa gttaatggtg gtgttagcgg
       61 ctgctttagc tacgggtgtg aaggcgggcg tatatagata tatatacaag gagaggatgg
      121 tgacaagagc aagagcaagc aaagcaaagc gggaaagctt tgttacgaga gaagaccgaa
      181 ctgctggatg caagttgtta gatgatggag gaagtacagc atgcaaaaga agcacctcgt
      241 ggatcatctg ctttgttgtc ttgtgggtag gtgcggcctt accacgacga cggaggagat
      301 gacgggtgcg tgaaagtatg tgactagtgc gggatagtct gttcactgcg gatatttatt
      361 ttttatttag gctgcactac attgcggatg taatgagcgt tttgttgcgg tttgtctgcg
      421 gatgactttg tttatttagt tttttgcttt tgacaaactt acttaccagc atatctgagg
      481 caacagagat gtacaagtcg ttttgtatac aaaaatagaa cacagaaaaa aacataaaaa
      541 cacataccta gtgtgtacaa tatgtagcgt tacttacatc gttacagtag caatatatag
      601 aacacagggc taccaacagt gcttcacact gtatacatta ttacaacaac aatatttgag
      661 ggggtggagt tatccacatg gatatagaga gtttaaaggt aattttgata attactggtg
      721 caacattact ctattacaaa gtatgaatac ttgtatgttt tgctggtttg cgtggtgttg
      781 ggtgtcccaa aagtaaaacc attagcagtt ggtgttgcaa atacttgtat actggcactg
      841 tctatgtctt ggccagtaat tactaccttg ggggtgttac cgaatgcttg attaaacgta
      901 acctctgcaa gcgttccagc cgtagtatta gcaccagcaa ctatagttat ggtaccggcg
      961 gtgtcgttac cttctatggt tacttgtggg gcagatatgt tgtttagggt gtcttctgta
     1021 cctgctgctg tacctggtgt tgctactgga gtattccctg ctgttatgat atggccgtta
     1081 actgttaggt tagctactgt gacattctgg actgttaggt tgccggctat gacgacatcg
     1141 tctgttacag ttaggctctt catagttact ggaccggtga catttaggtt acctactaca
     1201 tttaggttac cttgtatagt tccaccattg gttagatc
//
LOCUS       DAOPLS010000243         1224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_136982_length_1224_cov_3.633875, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000243 DAOPLS010000000
VERSION     DAOPLS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136982_length_1224_cov_3.633875"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..303)
                     /locus_tag="PKV52_04490"
     CDS             complement(127..303)
                     /locus_tag="PKV52_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99501.1"
                     /translation="MNVENCPISIAECVHPGLSFNYYQEDIAGAEDRRHKTFVQWTRQ
                     LGICQECLAGVDEE"
     gene            complement(306..1037)
                     /locus_tag="PKV52_04495"
     CDS             complement(306..1037)
                     /locus_tag="PKV52_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99502.1"
                     /translation="MMDKELKEAKKSIKKTRKLLTFLVGLLSVLLFTIVALVIWAIIQ
                     EQKTPTNFAECVLDKDAKILQSYPEQCVADGKSFTNPNQKVVQAEPPQEELSSDAWLL
                     YIPDDESYTVKLVDGWNYESYAVQKGGKETLVSCMGGCQEYKQSNPAQLVNKTGRYSG
                     NANVVITYGADPVLLASYMPANDIPLKNGEVAKKYVYNDGKQTTYLYVVKGKQTTVTV
                     QYTQENTVNSSTELIEEMVSTIAVD"
BASE COUNT          279 a          253 c          248 g          444 t
ORIGIN      
        1 gtggtgatgt aacttttggg gctttctttt ttaagctggg ttttttggtt ggttttttct
       61 ttgtaggcat tagcggtatt atagcaaata tgctgtagtg acagctaggc tgtaaggctt
      121 gttttactac tcttcgtcta cgccggctag gcactcttgg cagatgccta gttgccttgt
      181 ccactgtacg aacgttttgt gtctgcgatc ttctgcgcct gcaatatctt cttggtaata
      241 gttaaacgac aacccagggt ggacacattc cgcaatacta atggggcaat tttctacatt
      301 cataattaat ctacagctat tgtgcttacc atttcttcta taagctccgt actactattt
      361 actgtgtttt cttgggtgta ttgcacagtt accgtagttt gcttgccctt aacaacatac
      421 aaatatgttg tttgcttacc atcgttgtat acgtactttt ttgccacctc gccatttttt
      481 agtggtatgt cgttggctgg catgtagctg gcaagtaata ctgggtctgc accgtaggtg
      541 ataactacgt ttgcattgcc actatagcgc ccagttttgt tcaccagctg ggcagggttg
      601 gattgcttgt attcttggca gccgcccata caagatacaa gtgtttcttt gccacctttt
      661 tgtacagcgt aagattcgta gttccagcca tccaccagct taacagtgta ggattcgtcg
      721 tctggtatgt acagcaacca ggcatcagaa ctcagctctt cttgtggtgg ttctgcttgt
      781 actacttttt ggttggggtt agtaaaactt ttaccatcgg ccacgcattg ttctgggtaa
      841 ctttgtagta ttttggcatc tttatctagg acgcattcag caaaattagt aggagtcttt
      901 tgttcttgaa taatcgccca aattactaac gcaacaattg taaacaacag tacgctcaat
      961 agcccaacaa gaaaagttaa tagctttctg gtttttttta tacttttttt tgcctcttta
     1021 agctctttat ccatcatgtt tattataaca ccatagatat caaggcgctt gattgtttat
     1081 tgtgcaagaa ttacgttttc tggcataaac acacttatga gactttttct aagatcttca
     1141 tcctgcgcat tttcactaca tatgctgggg ctacctgtga cagtgaaata gtagttccca
     1201 acttttttat tagtccaagc ttgg
//
LOCUS       DAOPLS010000244         1220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_137761_length_1220_cov_2.293562, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000244 DAOPLS010000000
VERSION     DAOPLS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137761_length_1220_cov_2.293562"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PKV52_04500"
     CDS             complement(<1..1180)
                     /locus_tag="PKV52_04500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPD99503.1"
                     /translation="MLTEVAERAITKANDQPTNLIIEGDNYHSLSTLNYTHAGRVDFI
                     YIDPPYNTGATDWRYNNNYVDGADAFRHSKWLSFMKHRLELARNLLSDDGVICVTIDD
                     YEAPRLWVLLEELFGYENHLGTVVIRNNPKGRMTKRKFSLVHEYGIFFGKSSQSVIKK
                     LPVDPSEKTHNYKQDTDGSWYLPVNLRKQGVDSDAYNKKGELSHRYYPIYFDPETGAI
                     SVTKELPIKIDPIDVSGTKRIWRRGKEVIEKMYEDGDLWVQKTANGYQPYFKFRGGLD
                     GKLAQSVWAEAHFSASDYGTKILDSILGQREKFQYPKAPEAVKQSILSATNKKNAIIL
                     DFFAGSGTTGHAVLELNKEDGGNRSFILCTNNENNIAEEVTYQRVKGVMDGYKDVAGI
                     P"
BASE COUNT          304 a          278 c          248 g          390 t
ORIGIN      
        1 caggaatgcc agcgacatct ttatagccgt ccatcacacc tttgacccgt tgatacgtta
       61 cttcttcggc tatattattc tcattgtttg tgcaaagtat aaagctacga ttaccgccat
      121 cttccttgtt tagctccaag acagcgtgtc cagtagttcc agatccagca aagaaatcta
      181 ggataattgc attcttctta ttggtagcag ataagattga ctgtttgact gcttctggtg
      241 cttttggata ctggaacttc tcgcgttgcc caagaatact atcaaggatt ttagtgccat
      301 aatcacttgc cgaaaaatgt gcttctgccc atacggattg tgccaacttg ccatcaagac
      361 cgcccctaaa cttgaagtat ggctgatagc cgttcgccgt cttttgaacc cataggtcgc
      421 cgtcttcata catcttttca atgacttctt taccacgtcg ccatatacgc tttgtgccac
      481 ttacgtctat tggatctatc tttatgggca attcttttgt gacgctgata gcacccgttt
      541 cagggtcgaa atagatcgga tagtatctgt gcgataattc accctttttg ttgtatgcgt
      601 cactatctac accttgctta cgcaagttca ctggtaaata ccagctaccg tcagtgtcct
      661 gtttgtaatt atgtgttttt tcggacggat caacaggtag tttcttgatg acgctctgcg
      721 aacttttgcc aaagaatatg ccgtattcgt ggactaatga aaacttgcgt ttggtcattc
      781 gtccttttgg attgttacga atgacaacag tgccaagatg attttcgtag ccaaacaatt
      841 cttccagcaa tacccacaat ctaggtgctt cgtagtcgtc tattgttaca cagataaccc
      901 catcatcgga aagtaggttt ctagcaagtt ctaaacggtg tttcataaat gaaagccact
      961 tgctatgacg gaatgcgtca gcaccatcaa cgtagttatt gttataccgc cagtcagtcg
     1021 cacccgtgtt atatggtgga tctatgtaga taaaatctac cctgccagcg tgtgtgtagt
     1081 tcagtgtgga aagcgaatga taattgtcac cttcaataat caagttcgta ggctgatcgt
     1141 ttgcttttgt aatagcacgt tcggcaactt ccgttagcac gggtagttta gtgtcgcaat
     1201 caacaacaac ctgttcaggt
//
LOCUS       DAOPLS010000245         1212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_139114_length_1212_cov_2.681936, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000245 DAOPLS010000000
VERSION     DAOPLS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139114_length_1212_cov_2.681936"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PKV52_04505"
     CDS             <1..388
                     /locus_tag="PKV52_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99504.1"
                     /translation="NGITKMSLDIVLTSQDYNTNTRCPHCRSIYTETTYDTTYYADNI
                     THNLAPMAREAGLYDVIWEPKVNRAYDLAPLLQAGLSNLLSAPNHYKNLNPKNGYGCY
                     DDLVRFARRYLAACQENPYAWVRIYK"
     gene            462..656
                     /locus_tag="PKV52_04510"
     CDS             462..656
                     /locus_tag="PKV52_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99505.1"
                     /translation="MNKIVVLSVDDWEALYVNGVNVYEDHMIEVRDLAKYCPIENIKR
                     LWVGDTILVNGRFPKNLPVE"
     gene            974..>1212
                     /locus_tag="PKV52_04515"
     CDS             974..>1212
                     /locus_tag="PKV52_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99506.1"
                     /translation="MELETIYENLCVHDPRNPFYADDCEDTPEIGGDCYCDNCFYGRH
                     NLAVAILKLRDACWLALDEMCSIVAPSTRFTATAD"
BASE COUNT          403 a          213 c          236 g          360 t
ORIGIN      
        1 gaatgggatt actaaaatga gtttagatat tgttttaaca agtcaggact acaacacaaa
       61 cacccgatgc ccacattgta gatcaatcta tacggaaacc acatatgaca ctacttatta
      121 tgcagataac attacacaca accttgcgcc aatggcacgc gaagcaggtt tgtatgatgt
      181 aatttgggaa ccaaaggtta accgcgcata tgatttagcc ccacttttgc aagcaggttt
      241 gagcaaccta ttatcagcgc caaaccatta caaaaacctt aaccctaaaa atggctatgg
      301 ttgttacgat gacttagtaa ggtttgcaag gcgttactta gctgcatgtc aagaaaaccc
      361 gtatgcttgg gtgcggatat ataaataaag ggtttagtac caaaggcatc tttaccacta
      421 attaaaacat agctataatt gaaaatataa aggagaataa aatgaacaaa attgtagttt
      481 tatcggtgga tgattgggaa gcattgtatg taaatggcgt aaacgtttac gaagaccata
      541 tgattgaagt tagggactta gccaagtatt gtccgattga aaacatcaaa cgtctttggg
      601 ttggtgatac tattttagtt aatggcagat ttccaaaaaa tctaccagtc gaatagtacc
      661 aaatacccat agacaaactt atcgtgttta atgtaaaata taagttatcc acaatcaaaa
      721 gtatattctt tattaatagt agtagtaggg cttgtggaaa agtggataac tcatatttta
      781 cacctgtgtg ccagtctaat aacatgtgga taacttttaa taacttttcg gggaacccaa
      841 ttttgagcaa aagttatcca cagttttgaa aagttatcca catgttatcc acaggttatt
      901 aacaggccac attgttaaca gtagtaccaa atagctatcg acaaacttgc caaaaaggtt
      961 tattatttga tttatggaac ttgaaactat ttacgaaaac ctttgtgtac atgacccgcg
     1021 taatcctttt tacgctgatg actgtgagga cacccctgaa attggcggcg attgttattg
     1081 tgataattgc ttttatggta gacataattt agcagtagca atcctaaagt tgcgggatgc
     1141 ttgttggttg gcgctggatg aaatgtgtag catagttgca ccatctaccc gttttacagc
     1201 cacagccgat ca
//
LOCUS       DAOPLS010000246         1211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_139362_length_1211_cov_1.955882, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000246 DAOPLS010000000
VERSION     DAOPLS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139362_length_1211_cov_1.955882"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKV52_04520"
     CDS             <1..622
                     /locus_tag="PKV52_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99507.1"
                     /translation="TGQVDTRVVCTNTRRLAIRDLEPLPHTDDLGVYLRYITQAYLTA
                     LEQNYQRRATLDRLFTSKLPDVNKAIRTIFPTQTPGRNLRNLSKIVGFDIDTLVAKDT
                     IVTDDVEIQNLVDKEITRYRNDITRNEDRRSALADAFFAFNDTYPYAANTSYAMWNAT
                     TFVTSHGEDLTKANGKQLFYNDPSGLSLMYGERARINDRAWSYLTK"
     gene            619..834
                     /locus_tag="PKV52_04525"
     CDS             619..834
                     /locus_tag="PKV52_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99508.1"
                     /translation="MKYTQLYDYVTTLEKRIANLKNTNAIKMIDLVNILDDVGVFVGQ
                     QYPLLKQRFDSCVEQLEQLYVEEQNKE"
     gene            834..>1211
                     /locus_tag="PKV52_04530"
     CDS             834..>1211
                     /locus_tag="PKV52_04530"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD99509.1"
                     /translation="MELALKYRPKTPADFVGAQQQTAARVLQAAATSGAPYQKVLLTG
                     NSGLGKTSLALYYADLLDAEVHLINCGVSGGKANVEAIIGSFQMGSLSNQYKFYIFDE
                     IHNLTDSAQEALLADTEERLPPHV"
BASE COUNT          386 a          238 c          260 g          327 t
ORIGIN      
        1 aacagggcaa gttgatacac gggttgtttg tacaaataca cgtcgtttgg caattcgtga
       61 tttggagcca ttgccacaca cagatgatct tggggtatac ttacgctata tcacccaagc
      121 ctatctaaca gcgttagaac aaaattatca acgccgcgcc acgttagacc gtttatttac
      181 aagtaagttg ccggatgtca acaaagcaat tcggacaatt tttccgactc aaacccctgg
      241 gcgcaatttg cggaacctga gtaagattgt aggatttgat attgacacat tagtagcaaa
      301 ggatacaatt gttacagatg atgtagaaat tcaaaattta gtggataagg aaattacccg
      361 gtatcgcaat gatattactc gcaacgaaga tcggcgcagt gctttagccg atgctttttt
      421 tgcatttaat gatacctacc cttatgctgc caatacaagt tacgctatgt ggaatgccac
      481 cacatttgta acatcgcacg gtgaagattt aacaaaagca aatggtaaac agttgtttta
      541 caatgatccg tcaggcttat cattaatgta tggtgaacgc gcaaggatca acgaccgcgc
      601 atggagttat ttgacaaaat gaaatacaca caactatatg attatgtcac cacacttgaa
      661 aagcgtattg ccaatctcaa aaacacaaat gctattaaaa tgatagacct agtcaatatc
      721 ttagatgatg ttggggtttt tgttggacaa cagtaccctc tattgaaaca acgttttgat
      781 agctgtgtgg aacaacttga acaactatat gtagaggagc aaaataaaga ataatggaat
      841 tagcattaaa ataccggccg aaaacccctg ctgattttgt cggggcgcaa caacaaaccg
      901 cagcacgcgt attgcaagcg gctgcaacaa gtggtgcacc ttatcaaaaa gtgctactta
      961 ccggcaatag tggtttgggt aagacttcgc tggcactgta ttatgcggat ttactagacg
     1021 cggaagtaca tcttatcaat tgtggcgtta gtggaggtaa agccaatgtt gaggcaatca
     1081 ttggtagttt tcaaatggga tcgttgagta atcaatataa attttatatc tttgacgaaa
     1141 ttcacaactt gaccgacagc gcacaagaag cattgttggc cgataccgag gaacgtttgc
     1201 ccccacacgt g
//
LOCUS       DAOPLS010000247         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_139969_length_1207_cov_2.853299, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000247 DAOPLS010000000
VERSION     DAOPLS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139969_length_1207_cov_2.853299"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PKV52_04535"
     CDS             complement(<1..740)
                     /locus_tag="PKV52_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPD99510.1"
                     /translation="MTYYFDYAAATPVLPEVLTAMQPYFSEQFYNPSAIYMAARAVKK
                     DLQVARATVARQIGAKPSEIVFTAGGTEANNLAINGVASQYDNAHVVVSQIEHESVLM
                     PAKKHTTSFITVNSAGRIDIHSLPKIITDETVLVSVILANNEIGTIQDLRAVTTLAAE
                     ITKDRLKRGILRPLLVHTDACQAVNYVDIHVARLGIDLMTINSGKIYGPKQAGALFVK
                     TGTQLAPLILGGGQEHNLRSGTENVASY"
     gene            complement(767..1126)
                     /locus_tag="PKV52_04540"
     CDS             complement(767..1126)
                     /locus_tag="PKV52_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99511.1"
                     /translation="MGLFTTSQLSQKKQAVKYRELIRKEAVIGGKIFGPIPKNGRREF
                     FCLDERTWVWHEEWQDSAGTHHVLTTRYDVRPDGVVKTQNNSHYTKVSPTEAHHLLQA
                     ATLYKERVQSQLYADFQ"
BASE COUNT          295 a          262 c          255 g          395 t
ORIGIN      
        1 atgtagctag caacattttc tgtaccactg cgtaggttgt gctcttgtcc gccacccaga
       61 ataagcggag caagttgtgt accagttttt acaaataaag cccctgcttg cttggggcca
      121 tatattttac cagagttaat tgtcattaaa tcaataccga gtctagcaac atgaatgtct
      181 acatagttaa cagcctgaca cgcgtctgta tgaacaagga gtggccttag tatgcctctt
      241 tttagacgat cttttgtaat ctctgctgca agtgttgtta ccgccctcaa atcttgaata
      301 gtaccaattt cgttatttgc gagtattact gatactaaca ctgtttcatc tgtaattatt
      361 ttgggcaggc tatgtatatc aatcctccct gcactgttta cggtaatgaa gctcgtggtg
      421 tgcttttttg caggcattag taccgactca tgttcgatct ggcttaccac gacatgtgca
      481 ttatcatatt gcgaggcaac accgttgatg gctaagttat tagcctctgt gccacctgca
      541 gtaaagacta tttctgatgg ttttgcacct atctgccgag caacggtcgc acgggctact
      601 tgcaagtctt tttttacggc tcgtgctgcc atatagattg cacttgggtt ataaaactgc
      661 tcactaaaat acggttgcat tgccgtgagt acttctggca aaacaggtgt ggctgctgca
      721 taatcaaaat aataagtcat gattctatct tacctgtagt taatctttac tgaaaatcag
      781 cgtagagttg cgactgaaca cgttctttat acagtgtagc tgcttgtaat aagtggtgcg
      841 cttctgtagg gcttacttta gtatagtggc tattattttg agtttttaca acgccatcgg
      901 ggcgtacgtc atagcgtgtc gttagcacgt ggtgcgtgcc agcactatct tgccattctt
      961 cgtgccacac ccacgtacgt tcatcgaggc aaaaaaattc tcgccggcca tttttaggga
     1021 taggtccaaa aattttacca cctattactg cttcttttct aataagttcg cggtatttaa
     1081 ctgcttgttt tttttggctc agctgagaag ttgtaaacaa tcccatagct acctacttac
     1141 gctgcctgcc tatgagtagg tgccacgtca ggatttgctg cgatcattag gtctggaaaa
     1201 ttattaa
//
LOCUS       DAOPLS010000248         1205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_140421_length_1205_cov_1.825217, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000248 DAOPLS010000000
VERSION     DAOPLS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140421_length_1205_cov_1.825217"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..495)
                     /gene="rpsL"
                     /locus_tag="PKV52_04545"
     CDS             complement(76..495)
                     /gene="rpsL"
                     /locus_tag="PKV52_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPD99512.1"
                     /translation="MPTINQLVRKGRKSIAKKEKAPALRFTQNTLTGKTKRMKKGNPM
                     KRGVCTNVRTTTPKKPNSALRKIARVRLTNGIEVTAYIPGEGHNLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGALDSTGVDKRKRSRSKYGTKRPRS"
BASE COUNT          349 a          301 c          239 g          316 t
ORIGIN      
        1 cgcttctgaa aattatcgac accacaaacc catttgtgca gtgcaagcaa cgcatggata
       61 caacacgaaa acgcattaac tgcgcggccg ctttgtccca tatttactgc ggctacgttt
      121 gcgtttgtca acgcccgtgc tatccaacgc gccgcgaaca atgtgatagc gcacaccagg
      181 cagatctttc acacgaccac cgcgcaccaa gacgacactg tgctcttgta gattatggcc
      241 ttcaccaggg atataggctg ttacctcaat cccattggtc aaccgcacac gagcaatttt
      301 gcgcaaggca gaattcggct tcttgggggt tgtcgtacgc acgttggtgc aaactccccg
      361 tttcatcgga ttcccttttt tcatacgctt ggtttttcca gtgagcgtat tttgggtaaa
      421 acgtagcgcc ggggcttttt ctttctttgc tatactctta cggcctttgc ggaccagttg
      481 gttaatcgtt ggcaaaagaa tcctccaaat ttcatgtcac ttcaatttca catctgctga
      541 tactaaaacc gttgacgtta ctgcttcgag cagtaaacac ttactacgag tacatctgat
      601 ttaaccaggc atgacactat ccactagcac catcccaata tttattcacg agagcgatac
      661 tgcaacgcaa cagcaaaggc cagctgctct gcacatccag ctgaccacaa tagaaattat
      721 cgctaatgtt ctctgcagtg tctatatgtt aattctgtac ttttccgtcg aaaaacaaat
      781 aattactgga ctctggcgaa tacttgtatc gcagaaataa tgatcggtcg tcagtcgtgt
      841 gttgacggaa aaacgccaac gtccaataaa tattaacaat caaattcaat cgccatataa
      901 aaagcgagac ctaccaaggt taacccatct gcgatcattt ctgataaata tttcaaatac
      961 tgatcgaaat attgaacgat cgcaagcgta atagtactta tatagggtta cagggtgcgc
     1021 cgtaaacact atgccctaaa tcctgtagtg aagtgcctcc agtaaacaag acacacacca
     1081 gtaacaggca acatcaaatt tgtgggcatc attaacccac gaaaagaaag tgtatcataa
     1141 tagcgttggg gtggtcaaat cgcgaaagaa aacgcgcgca aatagttcta ctgcgatccc
     1201 atctg
//
LOCUS       DAOPLS010000249         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_141141_length_1201_cov_2.359511, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000249 DAOPLS010000000
VERSION     DAOPLS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141141_length_1201_cov_2.359511"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..>1201)
                     /locus_tag="PKV52_04550"
     CDS             complement(28..>1201)
                     /locus_tag="PKV52_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99513.1"
                     /translation="NGRVTSYNPGDGPSVEYSGEAVQNEASVSFTHYIRLVQTGLSAL
                     TSNETEDILSVAGITDEDCNRCGNGYPGADNVLVFGAGSSGGDANVLYTENGGGSILA
                     MSPDPWAGLNNEENTFIAIRPISQSQVRVIVATGTPATGANARFAYADLTYGAMGSPT
                     WTIVSIANTTVTTSAITAMLWATFDRNYIASEGDIYLSTDQGTTDPGLPIYTGAVAIN
                     GFAKAPDDSEVWAFGASNLILRELDQSGLFATRVGPAGGGAFTALAIAGDGTLYAGNG
                     TGIYKSTDKANVAGNWTELYDFGASHAVRAIQCIGGDRANGGDSQLLRVIVDDTTPGA
                     GTVWQTIDGGATWQPVTTLTNAGYNGAYFSKINDNKAIIVGDASGGAGVIHYLGPQ"
BASE COUNT          322 a          302 c          248 g          329 t
ORIGIN      
        1 tgaagccctt ttttgtttgt gttagggtta ttgtggccct agatagtgaa tcacaccagc
       61 acccccgctg gcatcaccga caataattgc cttattgtcg ttaattttgc taaaatatgc
      121 gccattgtaa ccagcatttg tcaatgtagt tacaggttgc catgtagcac caccatcaat
      181 tgtttgccat actgtgcctg caccaggggt agtatcatca acaattacac gtaatagctg
      241 actatcgccc ccgttggcac gatccccgcc gatacattgg attgcacgta ctgcatggct
      301 tgcgccaaag tcgtacaatt ctgtccagtt accagcaaca ttggctttgt cagttgactt
      361 atagatacct gtgccgttac cagcatacaa tgtaccatcc ccggcaattg ctaaagctgt
      421 aaatgcacca ccaccggctg gcccaacacg agttgcaaac aaaccagatt ggtcaagttc
      481 acgcaaaatc aagttgcttg caccaaatgc ccaaacttcg gaatcatcgg gtgcttttgc
      541 aaacccattg atagctacag cgccagtata gattggtaaa ccgggatcgg ttgtaccttg
      601 atctgttgac agataaatgt caccttcact ggctatgtaa ttgcgatcaa atgttgccca
      661 taacatagct gtaatagcac ttgtggtaac tgttgtgtta gcgatgctta caattgtcca
      721 agttggactg cccatagcac catatgttaa gtcagcataa gcaaagcgtg cgtttgcccc
      781 tgtagcaggt gtgccggtag caacgattac gcgtacttga ctttgactaa taggacggat
      841 tgcaatgaaa gtgttttctt cattgttcaa gcctgcccaa ggatcgggtg acattgctaa
      901 gatagaaccg ccgccgtttt ctgtgtacag cacattggca tcaccaccgc ttgatccagc
      961 accaaacact aacacattat ctgcaccagg ataaccatta ccgcaacggt tgcaatcttc
     1021 gtcggtaatg ccagcaacag acaaaatatc ctcggtttcg ttacttgtta aagcacttaa
     1081 accagtttgc accaatcgaa tatagtgtgt aaaggataca ctagcttcgt tttgtacagc
     1141 ttcaccgcta tattcaacac tcggcccgtc accaggatta tagcttgtaa cacggccatt
     1201 g
//
LOCUS       DAOPLS010000250         1199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_141441_length_1199_cov_3.458916, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000250 DAOPLS010000000
VERSION     DAOPLS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141441_length_1199_cov_3.458916"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKV52_04555"
     CDS             complement(<1..203)
                     /locus_tag="PKV52_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99514.1"
                     /translation="MTLILAGVLGTTPATAEEPDFGLTVWYDCDEDALVADFVLPANS
                     GTGSLMTFIGPYGDLVSMDGLVSG"
     gene            complement(430..921)
                     /locus_tag="PKV52_04560"
     CDS             complement(430..921)
                     /locus_tag="PKV52_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99515.1"
                     /translation="MSDYATKQDVQEIVDKAINKAVNSLAEIISDFATQVDERFEQVD
                     KRLDKVDERLDKVDKRLDMVDKRLDMVDKRLDKVESRLGSLESRVTNIETAQAQTNNR
                     LENIEGRLEALENDVKEIYKILAKKDPAVSKYQQQPLEDQIVEAYKAIVHIAKKANVS
                     LPK"
BASE COUNT          319 a          273 c          220 g          387 t
ORIGIN      
        1 cctgacacca aaccgtccat tgacaccagg tctccgtacg gtcctatgaa cgtcatcagg
       61 ctacccgtac ctgagttagc cggtagcacg aaatctgcta ccagggcatc ttcgtcacaa
      121 tcgtaccaga ctgttagccc aaagtcgggc tcttctgccg tagctggggt ggttccgagc
      181 accccggcca gaatgagcgt caccgttccc agcaacgccc aaaggatctt cggaatgttt
      241 ttcaacatcc gaatcacctc cttcctaatt gtcattcttc atcggccagg gtggccaagg
      301 aagcttctag gccctacatg tattgctaca catagaacac tcgtgtaata taataccagc
      361 ttttaactaa aaagtaaata gcaacgcgct tatgtagttg caaaaaatgt aatcgcgttt
      421 aaaaaatggt tattttggca aagatacgtt tgctttttta gcaatatgca ctatagcttt
      481 ataagcctct acaatttgat cttctaaagg ttgctgctga tacttactta cagctgggtc
      541 ttttttggcc aatattttat aaatttcttt cacatcgttt tctagtgctt ctaggcgacc
      601 ttctatattt tctaggcggt tattcgtttg cgcctgggcc gtttctatat ttgttacacg
      661 cgattctaaa ctacctaatc tgctttctac tttatctaaa cgtttgtcta ccatatctaa
      721 acgtttgtct accatatcta aacgtttgtc taccttatct aaacgttcgt ctactttatc
      781 taaacgtttg tctacttgtt cgaaccgctc gtctacctgg gttgcaaaat cactaattat
      841 ttctgctaag ctattgactg ctttgtttat agctttatca acaatttctt gtacgtcttg
      901 tttggtcgcg tagtcactca tattatagaa attgtagcac gcaagctacg ctttagtaaa
      961 aagcaatatt gcacagccgt tcttgcctaa atgttatctt ttaagtatac ttataaaata
     1021 actttaagga catactatga caattattgg cttaaaagaa ctttcacaaa acgctggtaa
     1081 aattgcagag cgtgtagcaa atggcgaacg ttttactgtt gttaaaaggt caaagccggt
     1141 gtttgagata ttgcccccta gcactacggc cgataacgaa ctggctgaat ggaccaagg
//
LOCUS       DAOPLS010000251         1198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_141596_length_1198_cov_3.185477, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000251 DAOPLS010000000
VERSION     DAOPLS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141596_length_1198_cov_3.185477"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..494)
                     /locus_tag="PKV52_04565"
     CDS             complement(69..494)
                     /locus_tag="PKV52_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD99516.1"
                     /translation="MIAVIIAGGSGTRLWPLSTKDYPKHLLSLTNDKSLLQNTVDRVQ
                     RLAKNDHIFVITEASHSKHVINQLEGLPKENILIEPGRRGTASCVLMGLNHVKKLGLP
                     KDEPIIFLWADHLIRDNDGFAASALRAGELSKGEKYLLE"
     gene            complement(509..754)
                     /locus_tag="PKV52_04570"
     CDS             complement(509..754)
                     /locus_tag="PKV52_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99517.1"
                     /translation="MSQDEFTKLFTYVSKKLDIIEDKLDDTAKKVDIQSILNRLDSIE
                     KQLEISEDERLVMGHQLDMVNRWVKEVADKIGYKLTV"
     gene            928..>1198
                     /locus_tag="PKV52_04575"
     CDS             928..>1198
                     /locus_tag="PKV52_04575"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015825388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPD99518.1"
                     /translation="MAKLLVTGGAGFIGANFVHFTLNNRPEHQVTVIDKLTYAGNPDN
                     VESILDKIEFITGDICDKDLMDDLVAKNDIVVHFAAESHNDNSLRD"
BASE COUNT          342 a          258 c          217 g          381 t
ORIGIN      
        1 gctcgcctgc ccttaaggcg ctcgaagcaa gggcgaaaag cggttagtat ttgcaggttg
       61 tgtgggctct actccaataa atacttttcg cccttgctta gctcgcctgc ccttaaggcg
      121 ctcgctgcaa agccatcgtt atcgcgtatt aaatggtctg cccacaaaaa tataattggt
      181 tcatcttttg gcaggccaag tttttttaca tggtttaagc ccatcaaaac acaactcgct
      241 gtgccgcgcc tacctggctc tatgagtata ttttcttttg gtagtccttc tagctgatta
      301 attacatgct tagaatgcga cgcttctgta ataacaaaaa tatgatcatt ttttgccaac
      361 cgctgtacgc ggtcaaccgt attctgcaac aagcttttat cattagtcag gcttaataag
      421 tgtttggggt agtctttggt gctaaggggc cacagccgcg tgccagaacc ccccgcaata
      481 ataactgcaa tcatagcctg ccatactcct agacggttag tttatatcca attttgtcag
      541 caacctcttt aacccatcgg ttgaccatat ctaactggtg acccataact aatctctcgt
      601 cttcagaaat ttcgagctgt ttttctattg aatcaagcct gtttaaaata ctttgaatat
      661 ctactttttt ggcggtgtcg tctagcttat cttcaataat atctaacttc ttagaaacat
      721 acgtaaaaag ttttgtaaat tcatcttgtg acatatatga attatatacc actctacgta
      781 taatcacatt gtctgttctc tttaatccgt taaatctctg aaaatgaata atgctatttg
      841 gtaacttaca tgttgttaaa tatacttatg caagcgaccc ctattaatac aacaaactat
      901 agtgactatt ggtagtacaa tatacatatg gcaaaactac ttgttacagg tggtgcgggg
      961 tttattgggg caaacttcgt gcatttcacc ctaaataatc gccccgaaca tcaagtaacc
     1021 gtaatagata aactcaccta tgctggcaat ccagataatg tagaatctat cttagataaa
     1081 atagagttta taactggtga tatctgcgat aaagacctaa tggatgattt agtcgcaaag
     1141 aacgatattg tggtacattt tgccgcagaa tcacataacg ataatagcct gcgtgacc
//
LOCUS       DAOPLS010000252         1188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_143441_length_1188_cov_2.872904, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000252 DAOPLS010000000
VERSION     DAOPLS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143441_length_1188_cov_2.872904"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..657
                     /locus_tag="PKV52_04580"
     CDS             130..657
                     /locus_tag="PKV52_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99519.1"
                     /translation="MHGNNDGLKRLFNYYRIKRRLQQPIATQVCPVCIEATCQHTYTH
                     VDTDTAKELLDWWIGVIEMELRHDTTVIDLVPRLVQLAELPTNYKTIFVNGVLGTRYN
                     GKLYLSDGDVLDVPGQGTQYGTYNIEMGFQPFATFNDGYINPVPYINNGVRALHIPND
                     AKIPCYKWGVQWAQV"
     gene            642..1004
                     /locus_tag="PKV52_04585"
     CDS             642..1004
                     /locus_tag="PKV52_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99520.1"
                     /translation="MGTGVAFYGEVDLFASDTLMAHVKDIMDRAVASGTTDFIFFDGT
                     YLDTLLRRVKYEGITKHFVSFRGLQPPEVSVTYLADLDWLYKFADYVVVINTKQPEKR
                     YNKRILWLHPDTLKEQWL"
     gene            1001..>1188
                     /locus_tag="PKV52_04590"
     CDS             1001..>1188
                     /locus_tag="PKV52_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99521.1"
                     /translation="MRITILTNRDSKWGLSRDAKIVSDLLLTRGHEVAIADFRNPPTG
                     QKAHVNIHLEIKAGRWFN"
BASE COUNT          384 a          212 c          262 g          330 t
ORIGIN      
        1 accaacacct aatcgtgcta ggggtaatgt attacctgta gaattcgaca taaatacacc
       61 ccctttggag ctagtcaagc aatatcataa gctaaatgac ttgccatttg atgaggaaat
      121 ggaactacta tgcatggaaa taatgacgga ctaaaacggt tatttaatta ttatcgaata
      181 aaacgccgct tacagcaacc aattgctacg caggtgtgcc ctgtttgtat tgaggcaacc
      241 tgtcagcata catacacaca tgtcgataca gatacagcca aagaattact tgattggtgg
      301 ataggtgtta ttgaaatgga attacgacac gatacgacag tgattgatct tgtgccacgt
      361 ttagtgcaac tagccgaact ccccacgaat tataaaacaa tatttgtaaa tggggtattg
      421 ggcacacgct acaacggcaa actgtatcta tcagacggtg atgttttaga cgtgcctgga
      481 cagggcacac aatatggcac atacaatatt gaaatgggtt ttcagccttt tgccacgttt
      541 aatgacgggt atataaatcc agtaccatat atcaataacg gtgttagggc attacatata
      601 cccaacgatg ctaaaatacc atgttataag tggggtgtac aatgggcaca ggtgtagcct
      661 tttatggcga ggttgattta tttgcaagtg ataccttaat ggcacatgta aaagacatta
      721 tggatagggc tgttgctagt ggcacaacag attttatctt ttttgatggc acgtatctgg
      781 atacgttgtt gcgtagagtg aagtacgaag gtataaccaa gcactttgta agttttcgcg
      841 gcttacaacc accggaagtc agtgttactt atttagcaga tttagactgg ctatataagt
      901 ttgcagatta cgttgtggtc attaacacaa agcaacctga aaaacgatac aataaacgta
      961 ttttatggtt acaccctgat acgttaaagg aacaatggtt atgagaatta caatactgac
     1021 aaacagagat agtaaatggg ggttgtcccg tgatgcaaag atagtaagtg atttattgtt
     1081 aacgcgaggg catgaagtag caattgctga ttttcgcaat ccaccaacgg ggcaaaaggc
     1141 acatgtaaat atacatcttg aaataaaagc agggcgctgg tttaacca
//
LOCUS       DAOPLS010000253         1172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_146488_length_1172_cov_3.248881, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000253 DAOPLS010000000
VERSION     DAOPLS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146488_length_1172_cov_3.248881"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..725
                     /locus_tag="PKV52_04595"
                     /pseudo
     CDS             54..725
                     /locus_tag="PKV52_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
     gene            733..>1172
                     /locus_tag="PKV52_04600"
     CDS             733..>1172
                     /locus_tag="PKV52_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99522.1"
                     /translation="MPLFQPAPPSPEQHKPASMEAQRSARLTEALDRLRAAVGNMDAN
                     AMQEGMRRTPAPVAPTDHHGLKPDSVAESNNLMKKAILEVNKAHEVHGAYSMPAEVVT
                     DESRTFAKHTTQQPSNVINLPTNRSDINQPTTYDDSSRGAQTAPP"
BASE COUNT          387 a          266 c          243 g          276 t
ORIGIN      
        1 tgcctgaaaa agaagcgcgc aaacgtgcag cagcagcagg cgttgataca atcatttgtg
       61 tcggaacaga tgttacaacg agctctcagg cggtaatgta tgccagcgat aaaccaactg
      121 tctgggcgag tatagggttg catccacatg atgccaagct tggtaatgac gcttttactg
      181 ctctcgccta attgctgcct aacaaaaaca tagtcgctat tggtgaatgt ggtctagatt
      241 atttctataa tcactcagac aaaaaagacc aatttgcagc acttgaaaac caaatgcaac
      301 tcgcccaaga taatacctta ccaatgattt ttcatgtacg tgaagccttt gatgattttt
      361 ggccaatata cgataatttt tcacgcctta aaggtgttgt gcatagcttt acagacacaa
      421 caacgcagct agataaagca cttaacagag acttatacat aggcttaaac ggtataatga
      481 catttacaaa agaccaaaaa caacttgccg cagccaaggc agtaccactg gagcgactat
      541 tattggaaac agatgcccca ttcttgacgc ctaagccact acgtggtaaa ataaacgagc
      601 ctaagaacgt gagtataaca gccgaatttc ttgcacagct aagaggtgag gaattatcta
      661 cgcttgcagc ggctacaaca aagaatgcgc gccaattatt tggtttgcct aagcatgagt
      721 cctaaataca ctatgccatt atttcaacca gccccaccat caccagaaca acataaacct
      781 gccagtatgg aagcgcagcg cagtgcgagg cttacggaag cgttagatag gctaagagca
      841 gctgtaggta atatggatgc aaatgctatg caagaaggaa tgcgtagaac tcccgcacct
      901 gtagcaccca cagatcacca tgggttaaag cctgatagcg ttgcagaatc aaataatctg
      961 atgaaaaaag caattcttga ggttaataaa gctcatgagg ttcatggagc ttatagcatg
     1021 cctgcagaag tagtaactga cgaaagtcgt acttttgcaa aacatactac gcagcaacct
     1081 agtaatgtca taaacttacc tacgaacaga agtgatatca atcaaccgac tacatacgat
     1141 gattcttctc gtggcgccca aacagcccca cc
//
LOCUS       DAOPLS010000254         1167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_147431_length_1167_cov_4.200540, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000254 DAOPLS010000000
VERSION     DAOPLS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147431_length_1167_cov_4.200540"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1018
                     /gene="mnmA"
                     /locus_tag="PKV52_04605"
     CDS             14..1018
                     /gene="mnmA"
                     /locus_tag="PKV52_04605"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPD99523.1"
                     /translation="MRQADGVTAALLKDAGYRVVGAYMKNWSKDLPGFECPWKEDYLD
                     AKRVAVQLGIDFQLFDFEEQYKSKVVDYMIEAYKVGTTPNPDIMCNQEIKFKLFFDTA
                     MLHGADYIATGHYARTTDGQLRTAKDDSKDQSYFLYRVNSATLKKTLFPLGDYKKTYV
                     RKLAAQYKLITATKKESMGICFVGKVGIKEFLSQYVTTQPGPVIDQYGNEIGTHDGAI
                     FYTIGQRHGLGVIGGLPVYVIEKDIDKNIVYVTSQLDDERLWRTSLRITSPHWINQAP
                     QLGKPYDIRTRHRAPLARASFKDTLDTLHVDSPVRAITAGQSVVIYEDDRVVGGGIVA
                     "
BASE COUNT          378 a          210 c          257 g          322 t
ORIGIN      
        1 ttttagttat aaactgcgcc aggctgatgg agttacagct gcacttttaa aagatgccgg
       61 ctaccgtgta gttggtgcgt atatgaagaa ctggagtaag gatttaccag ggtttgagtg
      121 tccttggaaa gaggactacc tagatgccaa gcgtgttgct gtacaacttg gtatagactt
      181 tcaactgttt gattttgaag agcaatataa atcaaaagtg gttgattaca tgatagaggc
      241 atacaaagtt ggcacaacac ccaacccaga cattatgtgt aatcaagaaa tcaagtttaa
      301 actatttttc gatactgcca tgctgcatgg tgctgactac attgctactg gtcactacgc
      361 ccgtaccaca gacgggcaac tacgtaccgc caaagacgat tcaaaagatc aaagctactt
      421 tttatataga gtaaacagtg caacactgaa aaaaacgtta ttcccgctcg gtgactataa
      481 aaagacgtac gtaagaaagt tagctgctca atataaactt ataaccgcta ctaaaaaaga
      541 aagtatgggt atatgttttg ttggtaaagt tggtataaaa gaatttttaa gccaatatgt
      601 tacgacccag ccagggcctg ttatagacca gtatggaaat gaaataggta cccacgatgg
      661 cgctatcttt tacactatag gccaacgcca cgggcttggt gtgattggtg gtttaccagt
      721 gtatgtaata gaaaaagata ttgataagaa tattgtatac gttacatcac agctagatga
      781 cgagcgtctg tggagaacat ctttacgaat aacaagccca cactggatta atcaggcacc
      841 gcaattagga aaaccttacg atatacgtac aagacatcgt gcaccactgg caagagcatc
      901 atttaaagat actctagata ctctgcatgt agatagccct gtacgggcta ttaccgctgg
      961 ccaatctgtt gttatttacg aggatgaccg tgtggtaggt ggtggtattg tcgcgtaacc
     1021 tctgtttatg tacatgctaa atatgatatt attaaacgga tatgcttagt aaactaaaag
     1081 ccaagaccaa ggagtctaaa cgaaaatctt cagatgataa attgatgatt gggcttgata
     1141 ttggtacgga atttgtgaaa gcattga
//
LOCUS       DAOPLS010000255         1165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_147793_length_1165_cov_3.663964, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000255 DAOPLS010000000
VERSION     DAOPLS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147793_length_1165_cov_3.663964"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="mltG"
                     /locus_tag="PKV52_04610"
     CDS             <1..634
                     /gene="mltG"
                     /locus_tag="PKV52_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPD99524.1"
                     /translation="IKAGFSEADVDTALDPAQYKGHPALVAKPTDQSLEGYLYPETFN
                     ASSKTTPKDIVEQSLDEMAEVLTPELIDKFQKQGLGIHEAVTMASIVLKESSNPEDQR
                     IIANVFFNRLQKGMNLGSDPTYQYIADTTGQPRSANIDSPYNTRKYGGLPPGPIGNVT
                     KSSLEAVANPASTDYLYFVAGDDGNIYYSKTLQEHEENVKNYCKELCANY"
     gene            939..>1165
                     /locus_tag="PKV52_04615"
     CDS             939..>1165
                     /locus_tag="PKV52_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99525.1"
                     /translation="MIHKKRSLFSKKRSRTVKRNFKKHGLLLANATVIALVTGFVWVG
                     HNSASQGSSATLSFIGQQSSTGESAPLDTLSS"
BASE COUNT          396 a          237 c          237 g          295 t
ORIGIN      
        1 cattaaagct ggtttttctg aagcagatgt agataccgca cttgaccccg cgcagtataa
       61 agggcatcct gcactcgttg ccaagccaac agatcagtca ttagaaggct atttatatcc
      121 agaaacgttt aatgcaagta gtaaaacaac tcctaaagat attgtggaac aatccctaga
      181 tgaaatggca gaggtattaa ccccagagct cattgataaa tttcaaaaac aagggcttgg
      241 tattcacgag gcagttacta tggcctcgat tgtcctaaaa gaatcatcca acccagaaga
      301 ccaacgtatc atcgcaaatg tcttttttaa cagattacaa aaaggtatga acttagggtc
      361 tgaccctacg tatcaatata ttgcagacac tacggggcag cctcgttcag ctaatattga
      421 ctcaccatat aacacgcgta aatatggtgg tttaccacct ggtccaatag gcaatgtcac
      481 aaaatcttca ctagaggcgg tcgcgaaccc tgcttcaact gattatttat actttgtggc
      541 aggtgacgat ggcaatatat attactcaaa aactctacaa gaacacgaag aaaacgttaa
      601 aaactactgt aaagaactgt gtgcaaacta ttagttaaca ggggtgaaag aagtttttaa
      661 ggcttgacaa caaggtaacg gtttgctaag ctaaaaagta cgatttttgg ttatagaaat
      721 gtaactaaag aacataaaga gggtccaaaa atcggtcgcg gctatcagat tatatggcgc
      781 ttagcaatgc cagggtattt ggcgagaaat accccattac aagaaagcat aaacgatagt
      841 gtcagcgaaa cgaccaatta tatggtcagg agatttgtat tagtaacgtt atcttggccc
      901 acaagccatc aacaacgact gtagcaaata ccaaaaccat gatccataaa aaacgatcat
      961 tatttagtaa aaaacgatca cgtactgtta aacgtaattt taaaaaacac ggattattgc
     1021 tggcaaacgc aacagtaatt gcgcttgtca ctggtttcgt gtgggtgggg cacaatagtg
     1081 ccagccaagg ctcaagtgct acgttgtcat ttattgggca gcaatcaagc acgggtgaat
     1141 ctgcaccact tgacaccctg tcatc
//
LOCUS       DAOPLS010000256         1159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_149012_length_1159_cov_3.076993, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000256 DAOPLS010000000
VERSION     DAOPLS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149012_length_1159_cov_3.076993"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PKV52_04620"
     CDS             <1..669
                     /locus_tag="PKV52_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD99526.1"
                     /translation="AASALRAGELSKGEKRLVFIGVEPTQPATSFGYMQKSGPVDGWF
                     NAFFLEQFKEKPDKKTADAYFHSGAYLWNTGYLVGSLRTFENTMQAVAPKLYQSLAKL
                     YNAPDFETAYLQLKNETIDTALSEHVTNGLVIPGTFDWVDVGSFSDLHSVSSTNDDGN
                     HVWGNGVELENTTNSYIRNDDTEPVAVIGLDNIVVVNTPNGVLVANKNYSQKVGDIAK
                     RIQS"
     gene            711..>1159
                     /gene="manB"
                     /locus_tag="PKV52_04625"
     CDS             711..>1159
                     /gene="manB"
                     /locus_tag="PKV52_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPD99527.1"
                     /translation="MQEILDEIFKAYDIRGKVGTELTPEVVEKIGYIFSNWLQGNGAI
                     AVGRDMRPDSAELANALINGVRKSGRDVIDIGQVTSDMIYFAVGNYHLAGGAMITASH
                     NPGEYNGIKLCKEEARPIGVESGLLDIKNEVLALTSLESDEYGNCIE"
BASE COUNT          389 a          199 c          281 g          290 t
ORIGIN      
        1 gcagcgagcg ccttaagggc aggcgagcta agcaagggcg aaaagcggtt agtatttatt
       61 ggagtagagc ccacacaacc tgcaacaagt tttggctata tgcaaaaaag cggtcctgta
      121 gacggttggt ttaatgcatt tttcttagag caatttaaag aaaaaccaga taaaaaaaca
      181 gcggatgcat attttcatag tggtgcatat ttgtggaaca caggttactt ggtaggctct
      241 ttgcgaacat ttgaaaatac catgcaagca gtagcgccaa agttgtacca atctttagca
      301 aaactatata acgcccctga ctttgagaca gcctatttac aacttaaaaa cgaaacaata
      361 gatactgcat tgtctgagca tgtgacaaac ggcttagtta taccaggcac ttttgactgg
      421 gtagatgtag ggagctttag cgacctgcat tcggtgagca gtactaacga cgacggtaac
      481 catgtttggg gcaatggtgt agaactagaa aataccacca atagctatat acgtaacgac
      541 gatacagagc cagttgcagt tattggacta gataatattg tagtggtaaa cacacccaac
      601 ggtgtactag tggctaacaa aaattacagc cagaaagttg gcgatatagc caagcgcata
      661 cagagttaga aattggatta tagattttag aagtaacaca ggagggacat atgcaagaaa
      721 ttttagacga gatttttaaa gcgtacgata tacggggtaa ggtaggtaca gaattgaccc
      781 cagaagttgt tgaaaaaatt ggctatattt ttagtaactg gctgcaaggc aatggtgcca
      841 ttgcggttgg ccgggacatg cgcccagatt ctgccgaact ggcaaatgca ctcattaatg
      901 gtgtaagaaa aagtgggcga gatgttattg acattgggca agtaacaagc gacatgattt
      961 actttgcggt gggtaactat catttggctg gtggtgcaat gataaccgca agtcataacc
     1021 caggtgaata taatggcata aaattatgca aagaagaagc aagaccaatt ggagtagaat
     1081 ctggactact agacataaaa aacgaagtat tggcgcttac ttcgcttgaa tctgacgaat
     1141 atggcaattg tatagaaaa
//
LOCUS       DAOPLS010000257         1157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_149531_length_1157_cov_1.472777, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000257 DAOPLS010000000
VERSION     DAOPLS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149531_length_1157_cov_1.472777"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PKV52_04630"
     CDS             complement(<1..957)
                     /locus_tag="PKV52_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99528.1"
                     /translation="MEWRKDFEIKSEYYGHFSPNASHFFDPRHYIQFGKIKAFKAITK
                     VTAQEKKDGIYIPRLTLIKGVRKGSVPITLNVEFSAPKVVNGNNYDELVDSDLDFLAE
                     QLKRKLTRYGVIVINNDTIKNAMVSTIHYSKNIPLTDYDTSYEVISDLEKCNFTTNKK
                     ADRQVYRGGGEAIHYYSSKWALCIYDKQKEYDMSKVSEKGLLEKDNYCQLSLFDDNPL
                     AKSVQMLRIEARYIGRQQIGKSLKDAGVAVNGYTLRDLFSEKISKTMLKHEMARLRDS
                     YPVMLLAKKSNRDLFLELQIQNPRKQLSTIMNAVAYKTLLDDT"
     gene            complement(944..>1157)
                     /locus_tag="PKV52_04635"
     CDS             complement(944..>1157)
                     /locus_tag="PKV52_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99529.1"
                     /translation="CGSVGIFSRPNGNGYRLVYPTRKMIGRDIDVYYPINRQVGKAIE
                     EAVASKFEEVVSKNNGRHDSDHFGMA"
BASE COUNT          276 a          263 c          240 g          378 t
ORIGIN      
        1 cgtatcgtct agtagcgtct tgtaagctac ggcgttcatt atggtagaaa gttgcttgcg
       61 tgggttctgt atctgtaatt ctaggaatag atcacggttt gatttcttgg ctagtaacat
      121 cactgggtag ctatcacgaa gcctagccat ttcgtgcttc aacatcgtct ttgatatttt
      181 ttcgctgaat aaatcccgta gggtgtagcc attcacagca acacctgcgt cttttagcga
      241 cttaccgatc tgctgtctgc caatataacg tgcttcaata cgcaacatct gaacagactt
      301 ggctaacggg ttatcatcaa acagcgatag ctggcagtag ttgtcctttt ctagtaagcc
      361 tttttctgac accttcgaca tatcgtattc tttttgttta tcgtagatac acaatgccca
      421 tttactgctg taatagtgga tcgcttcgcc accgccacga tacacctgtc tgtccgcctt
      481 tttgttggtg gtgaaattac acttttcaag atcactaatc acttcgtagg aagtgtcgta
      541 gtcggtaagc ggtatgttct tggaatagtg aatagtgctg accatagcgt ttttgatcgt
      601 gtcattgttt atcacgatca caccataacg ggttagtttg cgttttagct gttcggctag
      661 aaagtccaga tcactatcga ccagttcatc atagttattg ccgtttacta ccttcggggc
      721 tgaaaactcg acattcaggg taataggcac actaccttta cgcacgcctt tgatcagcgt
      781 caagcgtgga atataaatgc cgtccttctt ttcttgggca gtgactttgg taatggcttt
      841 gaaggctttt atcttaccaa actgaatata gtgacgtgga tcaaaaaagt ggctggcatt
      901 gggtgaaaaa tgcccatagt attcagactt tatttcaaag tctttacgcc attccaaagt
      961 gatcactatc gtgtctacca ttgttcttgc tcactacttc ttcaaactta cttgctactg
     1021 cttcttctat ggctttaccg acctgcctgt tgatcgggta atacacgtca atatcacgcc
     1081 cgatcatttt gcgtgtcgga tatacaagcc tgtaaccgtt gccgtttggt ctactgaata
     1141 tgcccaccga accgcaa
//
LOCUS       DAOPLS010000258         1153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_150217_length_1153_cov_2.553734, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000258 DAOPLS010000000
VERSION     DAOPLS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150217_length_1153_cov_2.553734"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1153
                     /locus_tag="PKV52_04640"
     CDS             <1..>1153
                     /locus_tag="PKV52_04640"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HPD99530.1"
                     /translation="MCQPDRFLDIIRSYIVFERDEEKHTYIKKLAAYHQYWAVNKALD
                     STLNSIQPESDHRIGVVWHTQGSGKSLSMVFYSGKLVIDQQMQNPTIIVITDRNDLDD
                     QLFGTFGNCNDLLRQIPLQADSRNNLQELLNQREAGGIIFTTNAKFFPEEGDDYPELS
                     NRSNIVVIADEAHRSQYNFIDGFAKHIRQALPNASFIGFTGTPIENDDRSTPAVFGNY
                     IDIYDIEQAVTDGATVPIYYESRLVELDIDKSMREQIDADIDDLLEAEDEAKQEEYKT
                     KNAQLEAIVGNGKRIERIASDIVQHFEERQEAMDGKAMIVTMSRRIAVDLYNAITDIR
                     PGWHSDDDMQGNVKVVMTGSASDPLDWQQHIRNKQRRGKLADRMKDTESD"
BASE COUNT          393 a          227 c          256 g          277 t
ORIGIN      
        1 atgtgccagc cagatagatt tttagatatt atacgcagct acattgtgtt tgagcgcgac
       61 gaagaaaagc acacttatat aaaaaaacta gcagcctacc atcaatattg ggccgtcaat
      121 aaagcattag attctacttt aaattctata cagccagaaa gcgaccatcg cattggggtt
      181 gtttggcata cacaaggctc tggcaaaagc ctaagcatgg ttttttatag cggtaaatta
      241 gtaattgacc aacaaatgca aaacccaaca ataattgtta ttacagatcg gaacgactta
      301 gatgatcagc tttttggtac atttggtaat tgcaatgacc tattgcggca aatacccctg
      361 caagctgata gccgcaataa cctacaagaa ttgctaaacc agcgcgaggc tggtgggatt
      421 atctttacta caaatgcaaa attcttccca gaagaaggag atgattaccc ggaactaagc
      481 aaccggagta acatagtagt aattgccgat gaagcacacc gtagccaata taactttata
      541 gacggttttg caaagcacat acgccaggcg ttgcctaatg ctagctttat tggttttact
      601 ggaacgccaa tagaaaacga cgacagaagc acgcctgctg tatttggtaa ttacattgat
      661 atttatgata tagagcaggc tgtaacagat ggtgctactg tgcctattta ctacgaaagc
      721 cgtttggtag agttagatat agataaatct atgcgcgaac aaatagatgc cgacatagac
      781 gacttactag aagcagaaga cgaagccaag caagaagaat ataaaactaa gaatgcccag
      841 cttgaggcta tagtgggcaa cggcaaacgc atagaacgca tcgctagcga cattgtacaa
      901 cactttgaag aacgccaaga ggctatggat ggcaaggcaa tgatagtgac aatgagccgc
      961 cgaatagctg ttgatttata taatgcaatt accgatatcc gcccaggctg gcatagtgat
     1021 gatgatatgc aagggaatgt aaaagttgta atgacaggta gcgcgtctga cccgctagac
     1081 tggcaacaac atattcgcaa caaacagcgg cgcggtaaat tagcagatag aatgaaagat
     1141 accgaatctg atc
//
LOCUS       DAOPLS010000259         1147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_151339_length_1147_cov_3.259158, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000259 DAOPLS010000000
VERSION     DAOPLS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151339_length_1147_cov_3.259158"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKV52_04645"
     CDS             <1..400
                     /locus_tag="PKV52_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPD99531.1"
                     /translation="LTLKALGEALYNLVSAFFSAITGNTKEARQEASKAGENVSGPVG
                     IFAILQQGTDLGYQFILFVVAVISLTLAIMNFLPIPALDGGRAFVMLLFKAMKKPLTP
                     DLEARIHGTGFAALMILFVVITVVDIRRFY"
     gene            413..>1147
                     /locus_tag="PKV52_04650"
     CDS             413..>1147
                     /locus_tag="PKV52_04650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HPD99532.1"
                     /translation="MDTLIKNKKRIVVPWDARYAYSVTILAYVVSQLFTTLPLLVALF
                     FTNWSTIEDSLLDQPWMSLSLTGVGAVGIFVVLYYFLHKKKYGFSQLKLQKGLPLKSL
                     LLVAGTYIVYILLSVAVAAIVQALFPSFNADQEQLVGYKNAVGWQLILAFVGLVVVPP
                     IAEEMLFRGFLYQGLRDHWNKHATLWWGFGVAIAVALLAGAVAGIIVALLIVVSIVIG
                     KKHPARAAAIVTSVLFGLVHMQWNISI"
BASE COUNT          292 a          213 c          269 g          373 t
ORIGIN      
        1 tcttacactt aaagcacttg gcgaagcact ttataattta gtttctgcat tttttagtgc
       61 aataactggt aacaccaaag aagctcggca agaagcctct aaagcgggcg aaaacgtgtc
      121 tggaccagta ggtatatttg ccatcttgca gcaaggaaca gatttaggct accaatttat
      181 actatttgtc gttgctgtta ttagccttac tttggctatt atgaactttt tgccaatccc
      241 agccttagac ggtggtcggg catttgtaat gctccttttt aaggcgatga aaaaaccact
      301 tactccagac ttagaagcac gtatacatgg caccggcttt gctgcgttaa tgatattatt
      361 tgttgtaata acggtggttg atatacgccg cttttattag cgattcatgc ctatggacac
      421 gctgataaaa aacaaaaaac gaatcgttgt accatgggat gctcggtatg cgtatagcgt
      481 cactattttg gcgtatgttg tttcgcagct ttttacaacg ctgccactac tcgtagcact
      541 gttttttacg aattggtcca caatagaaga tagtttatta gatcaaccct ggatgagcct
      601 gagtctaaca ggggtagggg cggttggtat ttttgttgtt ttgtattact ttttgcataa
      661 aaaaaagtat ggtttttcgc aactaaaact acaaaaaggg ttgccactta aatcgttatt
      721 gcttgttgca ggaacgtata ttgtgtacat cttgcttagt gttgccgtgg cagctattgt
      781 gcaggcgctg tttccatctt ttaatgcaga tcaagaacag ctggttggtt ataaaaatgc
      841 cgttggctgg caattaatct tagcgtttgt tggcttggtg gtggtgccac caattgcaga
      901 agagatgtta tttagaggct ttttatacca agggctgcgt gaccactgga acaaacatgc
      961 cacgttatgg tggggttttg gcgtagctat tgccgtggca ctactagcag gtgctgtcgc
     1021 cggcattatt gtagcgctgc tcatagttgt atctatagtt attggtaaaa agcatccagc
     1081 acgtgcggca gctattgtta ccagtgtgct ttttggcttg gtacatatgc agtggaacat
     1141 tagtata
//
LOCUS       DAOPLS010000260         1143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_152264_length_1143_cov_2.077206, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000260 DAOPLS010000000
VERSION     DAOPLS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152264_length_1143_cov_2.077206"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="PKV52_04655"
     CDS             <1..137
                     /locus_tag="PKV52_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPD99533.1"
                     /translation="LDTVELVMALEEEFGVEIQDDEAEKITTVQQAIDYINEHMQDAA
                     "
     gene            214..>1143
                     /locus_tag="PKV52_04660"
     CDS             214..>1143
                     /locus_tag="PKV52_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HPD99534.1"
                     /translation="MANRRVVVTGLGAVTPLGLTVDQTWQNILAGKSGAATVDFDTKD
                     LTTTFACCVKDFDPNLAMSSKEVKRTDTFIHYAAEASRQAIEDAGLEITDEVSVRAGT
                     AIGSGIGGLPWIEKAHSAILQSPRKVSPFFIPGGIVNMASGYVAMQYKLRGPNFAVVT
                     ACTTGTHSIGMAARMIMHGDADVMVAGGSEMSTDRLGLAGFGAARALSTRNDAPTKAS
                     RPWDVDRDGFVLGEGAGVLVLEEYEFAKKRGAKIYAELVGFGMSDDAYHMTSPCPDGE
                     GFMLSMQNTMRDAGINPEQIDYINAHGTSTPAAD"
BASE COUNT          310 a          203 c          297 g          333 t
ORIGIN      
        1 cactggacac agtagaatta gtaatggcac ttgaagaaga attcggtgtt gaaattcaag
       61 atgatgaagc agaaaagatt acaactgtgc agcaagctat cgattacatc aacgaacata
      121 tgcaagatgc tgcataattc aattatctcg atatttgtta atggctatgc agatttctag
      181 tgtagccatt tttttgtaag aaggaggctg ggtttggcaa atcgtcgtgt cgttgttacg
      241 gggttagggg ctgtaacgcc actagggtta actgttgatc aaacatggca aaatattctt
      301 gccggcaaaa gtggtgcagc tacggttgat tttgatacta aagacctgac aacaactttt
      361 gcgtgttgtg ttaaagattt tgatccaaac cttgcgatgt cttctaaaga agtaaagcgt
      421 acagatactt ttattcacta tgcggcagaa gcgtcgcgtc aagcaattga agacgctggc
      481 ttagaaatta ctgatgaagt gtcagtacgt gctggtactg caattggttc tggtatcggc
      541 ggtttaccat ggattgaaaa agcacatagt gctattttgc aaagcccacg taaagtttca
      601 cccttcttta ttccgggcgg tatcgtcaac atggcttcag gttatgtcgc catgcaatat
      661 aaacttcgtg gtccaaattt tgcagtggtg acagcgtgta cgacaggaac acatagtatt
      721 ggcatggctg cgcgcatgat tatgcatggt gatgctgatg tgatggttgc tggcggatct
      781 gaaatgtcga ctgatcgttt aggtttggct ggatttggtg cggcaagagc attgtctaca
      841 cgtaatgatg cgccgaccaa agcatcacgt ccttgggatg ttgatcgtga tggattcgta
      901 ttaggagaag gtgctggagt gctggtgctt gaagagtacg agtttgctaa aaaacgtggc
      961 gcaaaaattt atgccgaact ggttggtttt ggtatgagtg atgatgctta tcatatgaca
     1021 tccccttgtc ctgatggtga aggctttatg ctatcgatgc aaaacacaat gcgcgatgca
     1081 ggtattaatc ctgagcaaat cgattatatt aatgcacacg gtacgtcaac gccggctgcg
     1141 gat
//
LOCUS       DAOPLS010000261         1137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_153462_length_1137_cov_2.703327, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000261 DAOPLS010000000
VERSION     DAOPLS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153462_length_1137_cov_2.703327"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1039)
                     /locus_tag="PKV52_04665"
     CDS             complement(59..1039)
                     /locus_tag="PKV52_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HPD99535.1"
                     /translation="MILYLNDYTKKDIEKSPIRKGFGTGLVKAAEIDENVVALCADLT
                     ESTQMHLFRDAFSDRFFEVGIAEQNLATVGAGLAIAGKIPFISSYAAFSPGRNWEQIK
                     TTVALNDVPVKIVGSHAGLYTGADGATHQMLEDIALMRVMPNMVVIVPADAIEAEKAT
                     LAIAKDKRPAYLRLAREATPVISTTKTPFEIGKAYVYEPGDDITIISTGTMTYQALMA
                     AESLFKDGISAEVVHVPTIKPLDAVTILQSAKKTGAVITIEEAQIAGGLGGAVAEVLS
                     EHHPTPMRRLGVKDKYGESGSPDELLKHFGLTAKHIMLTAHELLSATGKQ"
BASE COUNT          291 a          258 c          228 g          360 t
ORIGIN      
        1 tgtcatgctt gaactagcaa ttatttgtag caccattacc tgttgccagg tcatggaact
       61 attgctttcc ggttgcagac aacaactcgt gtgctgtgag cataatatgc tttgctgtta
      121 ggccaaagtg ctttaaaagt tcgtctggtg acccactttc tccgtacttg tcctttacac
      181 ccaaacggcg cattggcgta gggtggtgtt cagacaatac ctctgccacc gcaccgccaa
      241 gtccaccagc aatttgggct tcttctatag taatcacggc acctgttttt ttggcacttt
      301 gcaaaatagt aacggcgtct agtggcttaa tcgtaggtac gtgtacaact tcggctgata
      361 tgccatcttt aaataatgat tctgcagcca tcaaagcttg atatgtcatt gtgccagtac
      421 tgataatcgt tatatcgtcg cccggctcat acacatacgc tttgccaatt tcaaaaggag
      481 tcttagtagt gcttattact ggtgtagcct cacgggcaag tcttagataa gctgggcgtt
      541 tatctttagc aattgctaag gtagcttttt ctgcttcgat ggcgtctgca ggaacaatca
      601 caaccatgtt cggcatcaca cgcataaggg caatatcttc gagcatttgg tgtgtagcac
      661 cgtctgctcc agtgtacaaa ccagcatgtg aaccaacgat ttttacaggt acatcgttca
      721 gtgctaccgt ggttttaatt tgttcccagt tgcgccctgg gctaaaggct gcatagctac
      781 taataaacgg aattttgcca gcaattgcta agcctgcgcc tactgttgcg aggttttgtt
      841 cggcgatgcc tacttcaaaa aacctatcgc taaaagcatc gcgaaacaaa tgcatttgag
      901 tgctttctgt taaatctgca caaagtgcca ctacgttttc atcaatttct gccgctttaa
      961 caagacccgt accaaaccct ttacgaatcg gggatttttc tatgtctttt ttggtatagt
     1021 catttaaata taaaatcatt tttttacttt cataattgca aatatactta aatagcctac
     1081 cgcagaccat ataataatat ctgtaagaag cattattggt tgcaagcctt gattttt
//
LOCUS       DAOPLS010000262         1133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_154365_length_1133_cov_2.030612, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000262 DAOPLS010000000
VERSION     DAOPLS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154365_length_1133_cov_2.030612"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..339
                     /locus_tag="PKV52_04670"
     CDS             37..339
                     /locus_tag="PKV52_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99536.1"
                     /translation="MNENTWLTVMQIAQVLLGLISGIGAVRIIDMIKNSLHIEGYWAT
                     LTTVTIAVVLAVLNLIVAEQLTPVPLTADSIVAIFGTIYAASQAEYIRLRKAQGAE"
     gene            402..725
                     /locus_tag="PKV52_04675"
     CDS             402..725
                     /locus_tag="PKV52_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99537.1"
                     /translation="MIINKYSSEQKLGLVSFTKLEELARYIEKHPTKKQVFVFDYLVD
                     ANNPMSWYVVSDILDITKDMLVVIAAAEFDDEPMTLRLVVKPNNRSKAAFVCRLLKDH
                     TPIRG"
     gene            728..1015
                     /locus_tag="PKV52_04680"
     CDS             728..1015
                     /locus_tag="PKV52_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99538.1"
                     /translation="MTYYIDCIGEQLVSNQFLPGIIKADSAYAPNPRTIVLTLNEDIS
                     GIVMRNFKDNVPVYAQYLSADDDVVKVVNAPVIALKTIPVDALWYTYITVI"
BASE COUNT          360 a          202 c          240 g          331 t
ORIGIN      
        1 aatgtcaact tttagcccat ttgatatact aaaatcatga acgaaaacac atggttaaca
       61 gtgatgcaga ttgcacaagt gttgcttggc ttaatatcag gtattggcgc tgtgcgtata
      121 attgatatga ttaaaaatag tttgcacatt gaaggatatt gggcaactct cacaacagta
      181 acaattgcgg tggtattagc agtgttaaat ctaattgtag cggaacagct aacacctgtc
      241 cctctaactg cggattcaat agtggcaata tttggtacaa tttatgccgc aagtcaagcc
      301 gaatatattc gtttacgtaa ggcgcaaggg gctgaataag cccctttttt ggtactaagg
      361 ttggctttac ctgaatcaga aatcgtgcta taatctaaat tatgataatc aataagtaca
      421 gtagtgagca aaaactaggc ttggtatcat tcacgaaact cgaagaacta gcgcgataca
      481 tcgaaaaaca cccaaccaaa aagcaagtgt ttgtttttga ttacttagtt gatgctaata
      541 acccgatgtc ttggtatgtt gtgtcagata ttttagatat tacaaaagat atgctagtgg
      601 taattgctgc tgctgaattt gacgacgaac cgatgacgtt gcggttagta gtaaaaccca
      661 acaatcgttc aaaagcggct tttgtctgtc gcttactgaa agaccataca ccaattagag
      721 gctaaacatg acatattaca tcgactgcat tggtgaacag cttgtaagca atcagtttct
      781 accgggtatt attaaagcgg acagtgctta tgccccaaat ccacgtacaa ttgtgttgac
      841 gcttaatgag gatattagtg gtattgtgat gcgtaacttc aaagacaacg taccagtata
      901 tgcgcaatat ttatctgctg acgatgacgt ggttaaagtt gtaaatgcac ccgttatcgc
      961 acttaaaaca atacctgtcg atgcgctttg gtatacgtac ataactgtga tataaatgaa
     1021 gcagtatttt tataccggta tacccgtgcg tgtgcagtca accagggaca aaaacgttgt
     1081 attgattaca gatgatgtgg gtaacgaaca ccttgtagca aaaaaggatt tgg
//
LOCUS       DAOPLS010000263         1132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_154462_length_1132_cov_3.873723, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000263 DAOPLS010000000
VERSION     DAOPLS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154462_length_1132_cov_3.873723"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..>1132)
                     /locus_tag="PKV52_04685"
     CDS             complement(201..>1132)
                     /locus_tag="PKV52_04685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPD99539.1"
                     /translation="PKIPGLQEIDYITYRTFAKQKTLPPSVAIIGGGTTAYEYAQIYA
                     AFGVKTHVFESHYHLFSSFDTEVGDLAESALTNTGVRIHTAANITKVHTTKNGNLITF
                     SQAGQEHHVSVDSIFVAAGNIPNTDIGLENAGVLNSEEGIRVNKRLQTSQKNIFAIGD
                     VTGNSHSASSAVHQGLVTAHNILHRKKIMFQDHAVPRVAYGMPEIVVVGKTERQLKLT
                     GLPYQTSIAPLGIVGKAHTSAYTSGFVKIVASYTGHVVGVSIVAPQASEFASELTFAI
                     QHRHRACDIANTIHPFSSWSEAVRVAASKIYCT"
BASE COUNT          312 a          228 c          243 g          349 t
ORIGIN      
        1 ggaatctatt tggatggagg tgaatcagaa attaagacag gctgccaaca aatagtttga
       61 atgccattct tctttgttcc ttcttttcta cttaccgcaa gtacgacccg ttctgtctta
      121 ctttgcttta agaagtactt aaaggagtga catatcttgc gtatactaat ataaccataa
      181 aaagacagat aagtacagca ctaagtgcag taaattttac tagctgcgac tcttacagcc
      241 tcactccagc tagaaaaagg atggattgtg tttgcgatat cacaggcgcg gtggcgatgc
      301 tgtatagcaa aagtgagttc actagcaaat tcactggcct ggggggcaac tatacttaca
      361 ccaactacgt ggccagtgta agaagcaaca attttaacga atccactcgt gtaggcgctt
      421 gtgtgtgctt taccaacaat acctagcggt gctattgaag tctggtatgg taaacctgtt
      481 agtttgagct gtctttctgt tttgcctaca acaactattt ctggcatgcc ataggcgact
      541 cgcggaacag catgatcttg aaacatgatt ttcttacggt gcagaatatt gtgggcggta
      601 acaagacctt ggtgtaccgc actgcttgcg ctatggctat taccagttac atcgccaatg
      661 gcaaagatat tcttttgtga tgtttgcagg cgcttattta cacgaatacc ttcttcagaa
      721 ttaagaacac cagcgttttc taaccctata tctgtatttg gtatattgcc cgctgctaca
      781 aaaatactat ctaccgatac atgatgttct tggccggctt gagaaaaagt aatgagatta
      841 ccgtttttgg ttgtgtgcac tttagtgatg ttcgcagccg tgtgtatccg tacgccagta
      901 ttggttaagg cactctctgc gaggtcacct acttcagtgt caaacgaaga aaataagtga
      961 taatgagatt caaacacatg tgtttttaca ccaaatgctg cgtatatttg agcatattcg
     1021 tacgccgtgg tacctccacc aataattgca acagacgggg gcagtgtttt ttgttttgca
     1081 aaagtcctat atgttatgta atcaatttct tgtaaaccag gtattttggg aa
//
LOCUS       DAOPLS010000264         1128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_155463_length_1128_cov_1.726002, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000264 DAOPLS010000000
VERSION     DAOPLS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155463_length_1128_cov_1.726002"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PKV52_04690"
     CDS             complement(<1..1113)
                     /locus_tag="PKV52_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99540.1"
                     /translation="MPEAKHYDVSELNQAFVYTNLSDININNNPEDLDFFKTKPTVHL
                     NEGITDVALPSVYLHRQNYGDLTASFIDVLVKALDARFGIEQGDMVNVAYEKADKDLE
                     LYDNELTNPILADVVLSGIDISRDQLGTLDLDTIQANVSTANIQREFDYLAKLWSLPF
                     APARSFTKVKSAIYKWFSHIGYEDAKWADVQRIIACSATNQRIFSEVIKTAQLDYERV
                     KAVELSQRRERKDFTFSLPKTDEFGESDEVVATSKYPYDVTYLKANRRDPERYFEDML
                     ERSDEIDWWYKNGEDKDRYFAIPYEATDEETGIKTLRGFYPDFIVRYKNGLVGIYDTK
                     AGFTVTDRKTYAKSDALQAYIAEHEGLTGGILNKRSD"
BASE COUNT          275 a          262 c          252 g          339 t
ORIGIN      
        1 gtcagatcgt ttgttcaaga taccaccagt caaaccttcg tgttctgcta tgtaggcttg
       61 aagtgcgtca gatttggcgt aggttttcct gtcggtaaca gtgaagccag ccttcgtatc
      121 atatatgccc acaagtccgt ttttgtagcg aactataaag tcagggtaaa agccacgtag
      181 cgtctttatg cccgtttctt catcggtagc ttcgtaaggt atggcgaagt agcgatcctt
      241 gtcttcgcca tttttatacc accagtcgat ttcatcagac cgttctagca tatcttcaaa
      301 gtagcgttcg gggtcacgcc tattagcctt caagtaggta acgtcatatg ggtatttact
      361 ggtcgctact acttcgtcac tttcgccaaa ctcatctgtt tttggcaagc taaatgtgaa
      421 gtctttgcgt tcacggcgtt gcgatagttc aaccgccttg acccgttcgt agtctagctg
      481 tgccgtcttg atgacttcgc taaatatacg ttggttcgtt gctgaacaag cgataatacg
      541 ctgaacgtca gcccactttg cgtcttcgta gccaatatga ctaaaccact tgtagatagc
      601 agatttgacc tttgtgaatg acctagcagg tgcgaatggc aagctccata gcttcgctag
      661 ataatcaaac tcacgctgta tgttagcggt agatacgttg gcttgtattg tgtccaaatc
      721 cagcgtgcca agttgatccc gtgatatgtc tatgccagac aacaccacgt cagcaaggat
      781 cggattagtc agttcgttat cgtataattc caaatctttg tcagcttttt cgtaggcaac
      841 attgaccata tcaccttgtt caataccgaa ccgtgcgtcc aaagctttta ccagcacgtc
      901 aatgaaacta gccgtcaggt cgccatagtt ttgacggtgt agatacacgc taggcaacgc
      961 aacgtcagta ataccttcgt tcaagtgaac cgtaggtttt gttttgaaaa agtccaagtc
     1021 ttcggggtta ttattgatgt tgatgtcgga caggttcgta taaacaaacg cctgatttag
     1081 ttccgaaaca tcgtagtgtt tggcttcggg catacgaagc acacgccc
//
LOCUS       DAOPLS010000265         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_156855_length_1121_cov_2.486867, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000265 DAOPLS010000000
VERSION     DAOPLS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156855_length_1121_cov_2.486867"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKV52_04695"
     CDS             <1..555
                     /locus_tag="PKV52_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99541.1"
                     /translation="TSTMPHKLKGTLISRFKSYHVELRLPTEAELKRHMLAIFEQEHI
                     TIDLHATEGRVVTPDGVVISPDYAKAVFERGEGNIRIIVNLIEQVLNGLFDVDNVTDL
                     STTDFDVVKAIYNADKQVFTHTIADYNAVTIGLCNYALGVLKRNDASTRVGARAVHVL
                     RTFGNGLNKNVPAHVAFWHLVASL"
     gene            565..807
                     /locus_tag="PKV52_04700"
     CDS             565..807
                     /locus_tag="PKV52_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99542.1"
                     /translation="MINFTTINVAVIPVTWDDPTLSNVLTPIADLDMWLRYLADDATI
                     PFYHTPAEIAAIEATVTAIKQTDFTTCAYFRFEKVV"
     gene            844..>1121
                     /locus_tag="PKV52_04705"
     CDS             844..>1121
                     /locus_tag="PKV52_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99543.1"
                     /translation="MTTLPTPIDGKAIRVYFDTEGNVLPGDAIKLYPSNIKEYLGSGG
                     NTFIEDAPADSRLYGRKNNTWTNFITFDDYVITVNEDGTADYETLDEA"
BASE COUNT          325 a          205 c          254 g          337 t
ORIGIN      
        1 acatcgacaa tgccacataa gttaaaaggc acgttaatta gccggtttaa gtcatatcat
       61 gtcgaattgc ggttgcctac tgaggctgaa ctaaaacgcc acatgttggc gatttttgaa
      121 caggaacaca tcacaattga tttacatgct accgaggggc gtgttgtaac acccgatggt
      181 gttgtgatta gtcctgatta cgctaaagct gtgtttgagc gtggcgaggg caatattcgc
      241 atcattgtaa atctgattga acaggtgttg aatggtttgt ttgatgttga taatgttaca
      301 gatttatcga ctacagactt cgatgttgtt aaagcgattt ataatgcgga caagcaagtg
      361 tttacacaca ctattgcgga ttataacgca gtaacaatcg ggctttgtaa ttatgctttg
      421 ggggtgttaa aacgcaatga cgccagtacg cgtgttggtg cgcgtgctgt acatgttttg
      481 cgtacctttg gtaatgggct gaacaaaaat gtacccgcac acgttgcgtt ttggcatttg
      541 gttgcaagtc tgtaagggta tactatgata aacttcacaa ctatcaatgt agcggtcatt
      601 cctgtaacat gggatgatcc cacattatct aacgtgctta caccaatcgc tgacttggat
      661 atgtggttgc gatatttggc agatgatgct acaatcccct tttatcatac ccccgccgaa
      721 attgcagcta ttgaagccac tgtaacggca ataaaacaaa ctgacttcac aacatgtgca
      781 tattttagat ttgaaaaggt ggtttagtta accacctttt ttactgtcta ttataatgtt
      841 ggtatgacaa cattaccaac accgattgac gggaaagcta tccgggtata ttttgacacc
      901 gaaggtaatg tattaccggg cgatgccatt aaattgtatc cgagtaacat aaaagaatat
      961 ttgggtagtg ggggtaatac gtttattgaa gatgcccctg ctgatagtcg cctttacggg
     1021 cgtaaaaata atacttggac aaattttatc acatttgatg attacgtaat aaccgtaaac
     1081 gaagatggca cagcagatta tgaaacgtta gacgaggcat t
//
LOCUS       DAOPLS010000266         1112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_158827_length_1112_cov_3.161779, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000266 DAOPLS010000000
VERSION     DAOPLS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158827_length_1112_cov_3.161779"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKV52_04710"
     CDS             complement(<1..547)
                     /locus_tag="PKV52_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99544.1"
                     /translation="MLRKYLFKQRTFMTTLVLSLVFYVLLMPFSAVLAANDSYVNVNR
                     DGWTFYLDGDRIIATNGTQYAFFNDAPENEKDVDKRFNQKKDFFKQQGEAFSCGSHID
                     RIKTETVNRGKTETKISYKLYEEGNGGPGCGQNTGYTIGDIKVEDSVPANVKKTAAAF
                     NYGNEPRGCPGSADIPNNANKK"
     gene            716..1096
                     /locus_tag="PKV52_04715"
     CDS             716..1096
                     /locus_tag="PKV52_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99545.1"
                     /translation="MKNLLTTIKLTLLATFFASTFIYAPINAQAQGLKPPDPTTSQVK
                     DSDLKNNPIIKWIEFFINVLSVTILAGGSVMIAWAGIQYMTARDNAQNVQAAKEKIWN
                     VIIGLLAYFFLYAFIQWLIPGGVF"
BASE COUNT          329 a          215 c          202 g          366 t
ORIGIN      
        1 tttttttgtt ggcattattg ggtatgtctg cagaccctgg gcaaccacgc ggttcattgc
       61 catagttaaa agcggcagct gtttttttga cattagctgg cacactgtct tctaccttga
      121 tatcgcctat tgtgtagccg gtattttgac cacaaccagg gccaccattg ccttcttcgt
      181 ataatttata tgaaattttt gtttctgtct taccgcgatt tacagtttct gtttttatgc
      241 ggtcaatgtg cgaaccgcaa gaaaaagctt caccctgttg cttaaaaaaa tctttctttt
      301 gattgaacct tttatctaca tctttttcat tttctggcgc gtcattaaag aacgcatatt
      361 gcgtaccatt ggttgcaatt attcgatcgc catccaaata aaatgtccaa ccatcgcggt
      421 tgacatttac gtacgaatca ttagccgcta gtactgcact aaatggcatt aacagtacat
      481 aaaataccaa actcagtacg agcgttgtca tgaatgttct ttgtttaaat aaatattttc
      541 ttaacatatt tgtattgtgt taccaaattt gagctttctt gtatataaaa gcacacggac
      601 atactattta ttaaaccata agagtcataa tctggctatg ctaataatta tagagtggcc
      661 tacttttgta taagacaatt ttatggttta atacactata gaaggtatat ataaaatgaa
      721 aaacttgctt actactataa aactgacgct acttgcgaca ttttttgcca gcacttttat
      781 atatgcgcca attaatgcgc aggcgcaagg tttaaaaccc ccagatccaa ccaccagcca
      841 ggttaaagac agcgacttaa aaaacaaccc aataattaaa tggattgaat tttttattaa
      901 cgtgctttct gtaacaatat tagctggtgg ttctgtgatg attgcgtggg ctggtattca
      961 gtacatgaca gcaagagata acgcacaaaa cgtacaagcg gctaaagaaa aaatatggaa
     1021 cgtaataatt gggctactgg cctacttctt tttgtacgcc tttatacagt ggttaatacc
     1081 ggggggagta ttttaatgca gctgcgagcc ag
//
LOCUS       DAOPLS010000267         1103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_160809_length_1103_cov_4.917939, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000267 DAOPLS010000000
VERSION     DAOPLS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160809_length_1103_cov_4.917939"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PKV52_04720"
     CDS             complement(<1..716)
                     /locus_tag="PKV52_04720"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPD99546.1"
                     /translation="MKKTIINDLNTAQRADVLLASAYPEYSRAALAKLFDMEQITKSD
                     VTIKAGDRIQPGVAIDADVSEILKKADVIELPIVYEDANVIVINKPAGIISHARGRYW
                     DEASVASFVRDRVSGLYGERAGIVHRLDRATSGVMVCAKNSETVAFMQKQFSSRNVYK
                     TYIAIVQKDVEPEAGIIDVPLARNPKNPKTFRPDPSGKEAKTEYKVVKSGETYSTLQL
                     HPLTGRTHQLRVHLQYIKHP"
     gene            complement(724..>1103)
                     /locus_tag="PKV52_04725"
     CDS             complement(724..>1103)
                     /locus_tag="PKV52_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPD99547.1"
                     /translation="TILLSDNPDEAAKKVMGATTDSVGKVNFDWQNQPGVTNLLQMLA
                     LLSGEPQESINAKWVGIVRYGDLKAAVAETTKNTLANLQSEYEATNDSQLQEKLTTSE
                     AIARQIADQTLLKVQQAVGLRPR"
BASE COUNT          266 a          247 c          214 g          376 t
ORIGIN      
        1 atggggtgtt ttatgtattg taagtgcact cgtagctgat gggtgcgccc cgttaatggg
       61 tgcaattgta aggtgctata cgtttcgcca ctcttcacta ctttgtattc tgtttttgct
      121 tcttttcctg atgggtcagg ccgaaacgtt ttagggtttt tggggtttcg cgctaatggt
      181 acatctataa taccagcttc tggttctaca tctttttgta caatcgctat gtacgtttta
      241 tagacattac gcgatgaaaa ctgtttttgc ataaaggcga cggtttctga atttttggca
      301 cacaccatga caccactcgt tgctctatct aaccgatgca caatgccagc gcgttcacca
      361 tataggcccg ataccctgtc acgtacaaaa cttgccaccg atgcttcatc ccagtaacgg
      421 ccccgtgcat gacttattat gcctgctggc ttattaataa caataacgtt agcatcttca
      481 tacactatag gaagttcaat gacatcggct tttttgagaa tttccgatac atctgcatca
      541 attgcaacgc caggttgaat cctgtcacct gcctttatag taacgtctga ctttgtaatt
      601 tgttccatat caaataattt agccaatgct gctcttgagt attcaggata agcactcgcc
      661 aataagacat cagctctttg agcggtattt agatcgttta taatggtttt cttcatacaa
      721 tcgttaacgc ggtcttaagc caacagcctg ctgtactttt agcaaagttt ggtcagcgat
      781 ctgcctggca attgcttcgc tagtggttag cttttcttgt aattgactat cgttggtagc
      841 ttcgtattca gattgtaaat tagccagagt atttttagtt gtttctgcta cggcagcctt
      901 taaatcgccg tatcttacta taccaaccca ttttgcgttt atactttctt gtggttcacc
      961 agaaagtaat gccaacattt gcagcaaatt agtaacacca ggctggtttt gccaatcaaa
     1021 atttaccttc ccaacactat cggttgtggc gcccataacc tttttggcag cttcatctgg
     1081 gttatcgctt aacaaaattg tac
//
LOCUS       DAOPLS010000268         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_162100_length_1098_cov_1.883030, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000268 DAOPLS010000000
VERSION     DAOPLS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162100_length_1098_cov_1.883030"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKV52_04730"
     CDS             complement(<1..593)
                     /locus_tag="PKV52_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99548.1"
                     /translation="MDIITDLAICEATIEAGLDTFYEVGNALRKIKDEKLWMGTTYTS
                     FEAYCTERWGFKLSNAYNLMSGSEVVNNLPSKPTRAAHVTPLKPLTPAHQSAAWTEVT
                     ANTSPDKITINQVNAVALKFRLVEELGADHWIVRQVNDGVMTSRYAWDVIKMLKTLPD
                     YYFTACDNIKALPSANTLGELRKLEYRQKDEVLGILQ"
     gene            complement(654..>1098)
                     /locus_tag="PKV52_04735"
     CDS             complement(654..>1098)
                     /locus_tag="PKV52_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99549.1"
                     /translation="GDEEPVLHILGGGAYSLRAAFYNGKVGGKWHNPAFEVGFIEQLT
                     DHRKKCRVPGGALPPTNAFSFKLGFADKPIVVGTGNKQQKINPLVRGGFKAVQPGLVA
                     KLNELYEAEKLDDWVNQFADGEVTLEPTNSVPEYVDADETDEIPF"
BASE COUNT          301 a          270 c          191 g          336 t
ORIGIN      
        1 ctttgcagta tacccaatac ttcatctttt tgccgatact ctaacttacg cagttctcca
       61 agtgtatttg ccgatggcaa agccttaata ttatcacacg cagtaaaata ataatccggg
      121 agtgttttaa gcatttttat gacatcccaa gcgtagcgcg atgtcataac cccatcattc
      181 acttgtcgta caatccaatg atccgcgcct aattcctcaa caagtctaaa tttaagtgct
      241 accgcattta cctgatttat tgtgatttta tcagggcttg tgtttgctgt aacttctgtc
      301 catgcagcac tttgatgtgc tggtgttaaa ggttttaacg gtgtaacatg tgccgcacga
      361 gtaggctttg aaggtagatt gttaactact tcactaccac tcattaagtt gtaagcattg
      421 gataacttga aaccccaacg ttctgtacaa tatgcctcaa acgatgtata tgttgtgccc
      481 atccacaact tttcgtcttt tattttacgt agtgcattgc ccacctcata aaaggtgtct
      541 aaccctgcct cgattgttgc ttcacatatt gctaaatctg ttattatgtc cacttgtctc
      601 cctaatacaa aaagccctgg acattgccag ggctttttgt gactcaaaaa aacttaaaac
      661 ggaatttcat cagtttcgtc agcatcgaca tattcgggta cgctgtttgt cggttccaat
      721 gtaacctcac catcagcgaa ctgattcacc caatcatcca acttttcagc ttcgtacaat
      781 tcgtttagct ttgcaactaa ccctggctgc acagccttaa aaccaccacg taccaacggg
      841 ttaatttttt gctgtttgtt accagtacca acaacaatag gtttatcagc aaaacctaac
      901 ttgaacgaaa acgcgtttgt tgggggcaac gcaccaccag gaacacgaca ctttttacga
      961 tggtctgtta gttgttcaat aaaaccaacc tcaaaagcag ggttgtgcca cttgccacca
     1021 accttaccat tgtaaaaagc agcgcgtaaa ctatatgcac caccgcccaa gatgtgtaac
     1081 acgggttctt cgtcacct
//
LOCUS       DAOPLS010000269         1097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_162207_length_1097_cov_3.027831, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000269 DAOPLS010000000
VERSION     DAOPLS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162207_length_1097_cov_3.027831"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1097
                     /locus_tag="PKV52_04740"
     CDS             <1..>1097
                     /locus_tag="PKV52_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99550.1"
                     /translation="MREFANVVAINPDNTITTIWDFAVDDATYAPVLSALATLVKSQP
                     VNGVFVSDKQKFGTKQSYDLAADSPVTVWNHFYIVTPERFVNKSHKACNKIASEYRDN
                     RAVLERSLNEISSDALNIVDDLIAENMLYRGQEWEAALNTFRTLKNEYNRLPDAKKAN
                     WLWRQAAQLNPAVSRIRNHSMGVLLQAISAGDDVGVAVRKYEAVVAPSNYKRPKPVIT
                     KQTIAEAEATVQRLGLLNSLGRRYARLDDITVRNVLWANRNAEKHMGGKGGVFDMLRD
                     DIVVAPQQYERATGMSVDRFVAEVLPQARTIDVLLENRHLSNMVSLIAPKVPNSKTMF
                     KWGNNFSWAYNGNMADSMKEHVKAAGGKVDGV"
BASE COUNT          334 a          212 c          269 g          282 t
ORIGIN      
        1 atgcgtgaat ttgccaacgt agtagcaatc aaccctgaca atacaattac aacaatttgg
       61 gattttgccg tagatgatgc aacatacgca cctgtgttaa gtgcattggc tacattggta
      121 aaatcacaac cagtaaacgg tgtatttgta agcgacaagc agaaatttgg cacaaagcaa
      181 agttacgatc ttgcggctga tagccctgta acagtatgga accattttta cattgttaca
      241 ccagaacggt ttgttaacaa atcacataaa gcctgtaaca aaattgcaag tgaatatcgt
      301 gacaatcgtg ctgttttgga gcggtcactt aatgaaattt caagcgatgc tcttaacatc
      361 gtagatgacc taattgcaga aaacatgttg tatcgtgggc aagaatggga agctgcgtta
      421 aatacattcc gcacattgaa aaatgaatac aaccgtttgc ccgatgcaaa gaaagctaat
      481 tggttgtggc gacaagctgc acaactgaat ccggctgtaa gtcgtattcg taaccattct
      541 atgggtgtac ttttacaagc gattagtgcg ggtgatgacg ttggggtggc tgtacgtaag
      601 tatgaagcgg tagttgcacc aagtaattac aagcgcccaa agccggttat cacaaaacaa
      661 acaattgccg aagccgaagc cacagtgcaa cgtttgggtt tattgaattc gttaggtcgc
      721 cgctatgcac ggcttgatga tattacagtt cgtaacgtgt tgtgggcaaa ccgtaatgcc
      781 gaaaaacaca tgggcggtaa aggtggcgtg tttgacatgt tgcgtgacga tattgttgtt
      841 gctccacaac aatatgaaag agcaacgggt atgtctgttg atcgctttgt tgcagaagtg
      901 ttgccccaag cacgcaccat tgatgtgttg cttgaaaacc gacacctatc aaacatggta
      961 tcactgattg cgccaaaagt gccgaacagt aaaactatgt tcaaatgggg caacaacttt
     1021 agttgggcat acaacggaaa catggccgat tccatgaagg aacatgttaa agcggcgggg
     1081 ggtaaggttg acggggt
//
LOCUS       DAOPLS010000270         1096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_162527_length_1096_cov_2.146974, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000270 DAOPLS010000000
VERSION     DAOPLS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162527_length_1096_cov_2.146974"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>1096
                     /locus_tag="PKV52_04745"
     CDS             36..>1096
                     /locus_tag="PKV52_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPD99551.1"
                     /translation="MLVVPLNSLIQFNAKDSHLGKIMATNNFIQTIFMLVFLIANIIF
                     MKLDGDIKHFLYILFMLAIAGTFFSIFSLPQALMRYVLYLIVSKLYRIKVNGLDNIPS
                     SGGVMLLGNHTSFLDWAILQIASPRPVRFVMERSIFEKWYLKWLLKKLRVIPISRGAS
                     KSALESINKALKVGDVVALFPEGRLSRNGQIGYFHTGFERAIAHSGAVIIPFYLLGLW
                     GTKNSYATEQYKSSSRSKHRYVSVSFGPALDDTINAITLKQKVMQLSIVAWNSYIKSL
                     GTIAEEWLKRVKQQPNQISVIDSLGTKFTNIQLLSTVMYMEEQLRDTLAFEQNIGILL
                     PSSAAGIITNLTLLCLGKTV"
BASE COUNT          324 a          227 c          189 g          356 t
ORIGIN      
        1 taatactatt cgttgtatat ggcttattcg gcggcatgct agttgtacca cttaactcat
       61 taattcaatt taatgccaaa gacagtcatc ttggcaaaat catggcaaca aataatttca
      121 ttcaaacaat ttttatgttg gtatttttga ttgcaaatat catttttatg aagctcgatg
      181 gcgatatcaa acactttctc tacattcttt ttatgcttgc tatagcaggt acatttttct
      241 caatttttag tttaccgcaa gccttaatgc gttatgttct ttatttaatc gtatcaaaac
      301 tttatcgcat aaaagtaaat ggattagaca acatcccttc tagcggtggt gtaatgttac
      361 tgggtaatca cactagtttt cttgactggg ctattttaca aatcgcctcc ccccgacctg
      421 ttcgctttgt catggaacgt agtatctttg aaaaatggta tttaaaatgg ctactaaaaa
      481 aacttcgtgt catccctatt tcaagaggcg cgagcaaatc agctcttgaa agtataaata
      541 aagccttaaa agttggcgat gtcgtcgccc tattcccaga aggcagatta tcgcgtaacg
      601 ggcagatcgg ttattttcac acgggatttg aacgtgccat tgcacattca ggtgccgtaa
      661 tcatcccctt ctatttatta ggactctggg gtactaaaaa ttcttacgca accgaacaat
      721 acaaatcatc gtctcgatct aaacatcgct acgtgagtgt cagctttggt cctgctctcg
      781 atgatacaat taatgccatt actctcaaac aaaaagtcat gcaactatcc attgtcgctt
      841 ggaatagcta tatcaaatca cttggaacca ttgctgaaga gtggcttaag cgtgtcaagc
      901 aacaacccaa tcagatcagt gtcattgact cgctcggtac taagtttact aatatacaat
      961 tactctcaac agtgatgtat atggaagaac aattaagaga tacacttgct ttcgaacaaa
     1021 atattggtat tttattgcct tctagcgctg ccggcattat cactaatcta acgttactct
     1081 gtctaggtaa aaccgt
//
LOCUS       DAOPLS010000271         1093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_163132_length_1093_cov_2.942197, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000271 DAOPLS010000000
VERSION     DAOPLS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163132_length_1093_cov_2.942197"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>1093
                     /locus_tag="PKV52_04750"
     CDS             171..>1093
                     /locus_tag="PKV52_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99552.1"
                     /translation="MDGITQLAQYFHRDDTPAERFDIDSVEQVVQGLKILNLLIQKLS
                     AAEAEPISDRKSFVEEDYMSSSASSDGSTARIMMRPTETPQAEARIRWAVNITPQEQM
                     EIFGRYLDVNSNGQPRNAKASLRLDFEKISGRLSLDLGSSMQYGSGSREYPDVLLAAL
                     ITAGVQHDALRQGKDIAHLDYHVRDTFDLSLSNPDTFAGFVRAMQERYVDKAKIELGR
                     MSVEATGLTAETTKESLPNVVYTGVFFDDAVLRSVAGVIFSEPYEHIPDNPHITLKFR
                     PKDGGLKGLDIGKQQKVRVVGYINDGKAQVL"
BASE COUNT          326 a          209 c          261 g          297 t
ORIGIN      
        1 gtggtaatga gatttttcgt ctaatatcgt cgattagaga aaacgcgcga gtatgggaat
       61 cgaatgcttg gtttgcccag ccagattgtg agtataatga atattttgca ggtatcaagc
      121 aagctataga gtcacgtata tcacagatat tgagccctac tccggatctg atggacggta
      181 taacgcaact ggcgcaatat tttcaccgtg atgacactcc ggctgaacgt tttgacatcg
      241 attcggtaga gcaagtggtg caagggctaa agatattgaa cttgctgata caaaaattat
      301 ctgccgctga agcagaacct atttctgatc gcaagtcatt tgtcgaagaa gattacatgt
      361 cgtctagtgc aagttctgat ggttctactg cacgcataat gatgcgacca actgaaactc
      421 cgcaagccga agcgcggata cgttgggctg ttaatatcac gccacaagag cagatggaaa
      481 tatttgggag gtatttagat gttaattcga acggtcaacc cagaaacgcc aaggcttctt
      541 tacgactaga ttttgaaaaa atttcgggta ggttatccct tgatctcggg tctagcatgc
      601 agtacggcag tggctcaagg gaatatcccg atgtattact tgcagcactt attactgctg
      661 gtgtgcagca tgatgccctg cggcagggca aagatattgc ccatttagac tatcacgtta
      721 gggacacatt tgatttatct ttaagcaatc cagatacatt tgcaggcttt gtcagagcta
      781 tgcaagaacg ttatgttgac aaagctaaaa tagaacttgg acgtatgtct gtagaagcaa
      841 ctggcttaac ggcagaaact accaaagaat cattgcctaa cgttgtgtat actggagtct
      901 ttttcgatga tgcagtactt aggtctgtag ctggagtaat ttttagtgaa ccgtatgaac
      961 atattcccga caatccccac attaccttaa agttcagacc aaaagatggg gggctaaaag
     1021 gtctagatat tggaaaacag caaaaggtaa gagtagtggg ctatataaac gacggtaaag
     1081 ctcaagtgct cat
//
LOCUS       DAOPLS010000272         1091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_163680_length_1091_cov_2.052124, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000272 DAOPLS010000000
VERSION     DAOPLS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163680_length_1091_cov_2.052124"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKV52_04755"
     CDS             complement(<1..528)
                     /locus_tag="PKV52_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99553.1"
                     /translation="MITIKLHANKQKIVGIYSPQPRSGKSTLANTIITNNFERHSFAT
                     TGKGMLNLLYTQLGYTPTEAYEMLNGNAKTQAVDGLYCDSRHLMQTLMTDWARQTIDP
                     GIWIRTMEQRLKTVKNPNIVIDDLRFPNEYHWIKSKGGFVICVLRPGIPGYEHASEGR
                     LNHYPFDTIINNTGTL"
     gene            complement(525..>1091)
                     /locus_tag="PKV52_04760"
     CDS             complement(525..>1091)
                     /locus_tag="PKV52_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99554.1"
                     /translation="MQSIQQHGFREPVGFDDTLGAIVSGNGRVEALWYLYQEWVKNDT
                     VVPDGIVVNADGMWEVPVIYGVNAANVKAAIAWLIDANNLTMAGGHFTALDMARAWDM
                     EGYLALLAESRNYAVSVDEADFQLMLQLTNGDTDEPMPESTPEIPDEAYHYVKIAFSS
                     MGDRDAFDDYMRDIPYEERGMYLLDLLK"
BASE COUNT          304 a          261 c          191 g          335 t
ORIGIN      
        1 caatgtacca gtgttgttaa tgattgtatc aaatggataa tggttaagtc gcccttcact
       61 ggcatgttca tatcccggta tacccggacg taaaacacaa atcacaaacc cccctttcga
      121 cttaatccaa tgatattcat tggggaagcg cagatcgtct atgacgatat tggggttttt
      181 aacagttttc agccgttgtt ccattgtccg aatccatata cccggatcaa tagtctgcct
      241 agcccaatct gtcatcaagg tttgcattaa atgtcgggag tcacaatata aaccgtcaac
      301 tgcttgcgtt ttggcattgc catttaacat ttcatatgcc tcagtaggcg tataaccaag
      361 ttgtgtatat agcagattta acatcccttt gcctgttgta gcaaagctgt gccgttcaaa
      421 gttgttagtt atgattgtat ttgccagcgt actcttaccc gatcttggtt gtggactgta
      481 aatgccaact attttttgct tattagcatg taatttaata gtaatcattt gagtaaatcc
      541 agtagataca tgccacgttc ttcatacggt atatcacgca tgtaatcatc aaaggcgtct
      601 cgatccccca tactcgaaaa agctatcttt acatagtgat atgcttcatc tggtatttca
      661 ggtgtggatt cgggcatagg ttcatctgta tcgccattag taagttgtaa cattaactga
      721 aaatcagctt cgtctactga aaccgcataa ttacgacttt ctgccaataa cgcaagataa
      781 ccttccatat cccacgcacg tgccatatct agtgctgtga agtgcccacc tgccattgtt
      841 aaattgttag catcaatcaa ccatgcaatt gctgccttta cattagcagc atttacacca
      901 tatataaccg gcacttccca catgccatca gcgttaacaa cgataccatc gggtactaca
      961 gtatcgtttt taacccactc ttgatacaaa taccacaacg cctcaacacg cccattacca
     1021 cttacaatag cgcctagtgt atcatcaaag cccacaggtt cccgaaaccc atgttgctgt
     1081 attgattgca t
//
LOCUS       DAOPLS010000273         1088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_164319_length_1088_cov_2.928364, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000273 DAOPLS010000000
VERSION     DAOPLS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164319_length_1088_cov_2.928364"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKV52_04765"
     CDS             complement(<1..382)
                     /locus_tag="PKV52_04765"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD99555.1"
                     /translation="MNPENEPIIPIESFNVTELCKNAERDGVTHISTGVAVVKDGRLL
                     LVRRVKGDYLGGNYELPGGGVDKGESLVQSALRELQEETGLVVKEVLGTFTGFDYTTD
                     KKPHVRQINFLVTVQNGAIILNPLE"
     gene            complement(423..836)
                     /locus_tag="PKV52_04770"
     CDS             complement(423..836)
                     /locus_tag="PKV52_04770"
                     /inference="COORDINATES: protein motif:HMM:NF026989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPD99556.1"
                     /translation="MGQDKKQWYLYVLKLEKDKYYVGITSQTVEKRFNEHKTGIRAAN
                     WTKKYKPVQIIHSRQLGVMNKADAEAIENKTVREYINTYGINRVRGGDITETIDLFIF
                     RRNIIDKQYTLDILQAILFGALMTLFIYYSLNTLL"
BASE COUNT          282 a          215 c          189 g          402 t
ORIGIN      
        1 gttctaatgg gtttaggata atcgcaccat tttgtactgt aactaaaaag ttaatttgac
       61 gcacgtgggg ctttttatct gttgtgtagt caaagcctgt aaaggtacct aagacttctt
      121 tcacaactaa gccggtttct tcttgcagct cgcgcaatgc agattgcact agcgattcgc
      181 ccttatctac cccaccacca ggtagctcgt aattaccgcc taggtaatca ccctttaccc
      241 tacgcactag tagcaatcgg ccatctttaa ctactgcaac acctgtagaa atatgtgtaa
      301 cgccatctcg ttctgcattt ttacagagtt ctgttacgtt aaaactttca attggtatta
      361 ttggctcatt ttctggattc atttgtagct attctacccc tgttctctgg tatttgccac
      421 agctaaagta atgtattaag gctatagtat ataaataatg tcattaatgc accaaagaga
      481 atagcctgta aaatatctaa agtgtattgt ttatctataa tgtttcttct gaaaataaat
      541 aagtctattg tttctgtaat atcgccaccg cggactctat ttatgccgta agtatttata
      601 tattccctta cggttttatt ctcaattgct tcggcatccg ctttattcat gacgcctaat
      661 tgtcttgaat gtattatttg aactggtttg tatttttttg tccaatttgc cgctcttatg
      721 ccggttttat gttcgttgaa tcttttttct actgtttgcg aagtaatgcc aacatagtat
      781 ttatcttttt ctagctttaa aacataaaga taccattgct ttttatcttg tcccataaaa
      841 accattctac ccctgttcgg ctgtttttac tacaaactct tgtagtattt ttctatcttg
      901 gtagccatta atcgcctacg ttcagacaag aaaggttcaa agtctttata agtagcatca
      961 aatattaatt ggggtatggc gttggcttgt aagtttttat tacagcaagt tctggggttg
     1021 gcaaaacagc tgtcgttggt gagctcgcaa tagaaaaaca tctatatgat gatggtctgc
     1081 ctttgtgt
//
LOCUS       DAOPLS010000274         1085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_165159_length_1085_cov_1.998058, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000274 DAOPLS010000000
VERSION     DAOPLS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165159_length_1085_cov_1.998058"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PKV52_04775"
     CDS             complement(<1..1067)
                     /locus_tag="PKV52_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99557.1"
                     /translation="MIDADPSQGQDATDITKREYDVDVPEGLQKFFASEIIKKANVAT
                     SIISILDSVARINQSISDGSLITMVNMAKAAQAIGVFQVMTTARDQSKTGELTADEYG
                     EFMKIINNATNNEGWQTVIEDSPTGSVRAETNGVFTAAKDKEEYCSEEHQAELHDPAN
                     AKAAESEHHYHCDDKKIGSATGAENITKWWNVFFKPFQPLLDAYMAIPLSNLVGFVNN
                     IADSVTGPIISTAIKVVGLESTKDAILGYMTTRITSALGAGPMLDTFTPGGQIMNVMI
                     EGSAASAESSARYQGGVATTALTMDYTNSLIAEYNNEKKYDSNIQKYLALSDPDSVLS
                     KTLFNQVKDINTSDRANSVSQ"
BASE COUNT          266 a          232 c          211 g          376 t
ORIGIN      
        1 tgttgagaaa cgctattagc acggtcgctg gtattaatat ctttcacttg gttaaataat
       61 gtttttgata gtaccgaatc aggatcagac aatgcgagat atttttgtat attagaatcg
      121 tatttttttt cgttgttata ttctgcgatc aaactgtttg tgtagtccat ggttagcgct
      181 gtggttgcaa caccaccttg gtagcgagca gaagattcag ctgatgcagc tgatccttct
      241 atcatgacgt tcataatttg cccacctggt gtgaatgtat caagcatggg gccagcgcca
      301 agtgccgagg ttattcttgt ggtcatatag cccaaaatag cgtctttagt actttctagc
      361 cccactactt taatggcagt acttataatt ggaccggtga ctgagtcagc tatattattc
      421 acaaaaccaa ccaaatttga aagcggtatt gccatatagg catctaacaa aggttggaat
      481 ggtttaaaga aaacattcca ccactttgta atattttctg cgcccgttgc actacctatt
      541 tttttgtcat cacaatggta atggtgttca ctttctgctg cttttgcatt ggccgggtca
      601 tggagctccg cctggtgctc ttcgctgcag tattcttctt tgtctttggc tgcagtaaac
      661 acaccatttg tttctgctct cacgctccct gtgggactat cttctattac cgtctgccat
      721 ccctcattat tagttgcatt atttattatc ttcataaact cgccatattc atcggctgtt
      781 agttcaccag ttttgctctg atctcgtgca gtagtcataa cttgaaacac accaattgct
      841 tgggcggctt ttgccatatt taccatggta ataagacttc cgtcactaat actttggtta
      901 atgcgtgcaa cagaatcaag aatagatata atactagttg caacattggc tttctttatg
      961 atttctgatg caaagaattt ttgcaaacct tcaggtacat ctacatcgta ttctcttttg
     1021 gtgatatctg tagcatcttg gccttggcta gggtctgcgt caatcacatt accatcagca
     1081 tcagt
//
LOCUS       DAOPLS010000275         1085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_165189_length_1085_cov_1.750485, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000275 DAOPLS010000000
VERSION     DAOPLS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165189_length_1085_cov_1.750485"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..>1085)
                     /locus_tag="PKV52_04780"
     CDS             complement(92..>1085)
                     /locus_tag="PKV52_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99558.1"
                     /translation="DNEFWVLAKIDQPGSDDENTSATKQIELMRYTDGRLDTPSSIRE
                     ISIPNAMAMKKYKDGMIIYSKDDVDAYFLSSFEAQPQKINFAELDKIDGYGFDIEIYN
                     NQISYYYKTHIRDTGNTAEINNLPITMVTDLKSPQLIDTPYIYSYAFACGDKNICIGL
                     ADENELVIVSGSSKKSVFNVRDYTIINNSLLLQIESTFVLYDVGDNSGTIVYQGNEYN
                     TPCGIQSTFSGVRICVDAKKTGNLYSVQLNLNSPKSTNIIDVINQISENNYVDSVTVL
                     GNIIYINDAVEPVYNEIIGEYLDDPEESAYAEQSIRQTVSETGVDTNKYSVVIL"
BASE COUNT          318 a          210 c          161 g          396 t
ORIGIN      
        1 aacctattta acaggtcatc aaacgattat taccaccacc gacgcagatg ttatagccaa
       61 agactttgcc caacacgcca acaccatcag tttataagat gacaaccgaa tacttattgg
      121 tatcaacacc agtttcacta actgtttggc gaattgattg ctcagcataa gcactttctt
      181 ctgggtcatc aagatattca ccaataattt cgttgtagac aggttctact gcatcgttta
      241 tatatattat atttcctaat actgttacag aatcaacata gttattttct gatatttgat
      301 ttattacatc tatgatatta gtacttttcg gtgaatttag gtttaattgc actgaatata
      361 aattacctgt ttttttagca tctacacata tccgtacgcc agaaaatgtt gattgaatac
      421 cacaaggtgt attgtattca ttaccttgat acacaatcgt accactatta tcaccaacat
      481 catacaaaac aaaagtactc tcaatttgta acaataaact attgtttata atcgtgtagt
      541 cacgtacatt aaatacggat tttttactag acccacttac tataaccaac tcgttttcgt
      601 ctgctaatcc tatacatatg tttttatcac cacatgcgaa agcataacta tatatataag
      661 gggtatcaat taattgtggc gactttaaat cagttaccat ggttatgggt aaattgttaa
      721 tttcagctgt gttacctgtg tctctaatgt gggttttgta ataatagctt atttggttgt
      781 tgtatatttc tatatcaaac ccatagccgt caattttgtc taattctgcg aaatttatct
      841 tttgtggttg cgcctcaaaa ctactaagga aatatgcgtc tacgtcatct ttgctatata
      901 taatcatacc gtccttgtat tttttcattg ccatagcgtt aggtatagat atttctctga
      961 tagatgatgg ggtgtctaat cttccgtcgg tatatctcat aagttctatc tgcttcgttg
     1021 cactcgtgtt ttcatcgtca ctacctggtt gatctatttt agcaagcacc cagaattcat
     1081 tgtca
//
LOCUS       DAOPLS010000276         1083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_165447_length_1083_cov_5.522374, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000276 DAOPLS010000000
VERSION     DAOPLS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165447_length_1083_cov_5.522374"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..>1083)
                     /locus_tag="PKV52_04785"
     CDS             complement(163..>1083)
                     /locus_tag="PKV52_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPD99559.1"
                     /translation="VKKYIYPLQHSRHRIVIVSVSILVALIVGFATYTILSLYKFQST
                     SAFAYQITKVVPLPFAKVDGTYVPYEEYLFELRHLIHFFEEKGGVDFTSEQGAQQLQE
                     EKKKIRDLIINNTYAEKIAREKGITVSEDEVNSQIDTLKKLGLLGSEQQVFEEVLRSN
                     YDWSIADFRRSIKQQLLNAKVVQALDPSVRAKADTVLAEISAGKDFATAAQESSDDIG
                     TKENGGNLGIVDANKAYLPQEYEALTAMQPGDVSGVIVLDNGLAIVKHLGFEGEKIKA
                     AHIVFKYKELAEYLNDYKAQKPATVYVKID"
BASE COUNT          321 a          227 c          184 g          351 t
ORIGIN      
        1 cgcctacact acaggcaatt gttggatact tttatagcac catgctccac tattacttat
       61 atacaaacaa cgcgattgaa gaacttgtta gctgaacacg ctttagtata ttactactac
      121 ggctaaacgt tcgctaaaaa catactgttg ctagaaagta tattaatcta tttttacgta
      181 aacagtcgct ggtttttgag ctttgtaatc attaaggtat tctgccaact ctttatactt
      241 aaatacaata tgtgcagctt tgattttttc gccctcaaag ccaaggtgct tgacgatagc
      301 taagccatta tctaatacta ttactcctga aacatcgccc ggctgcatag cggtaagagc
      361 ttcgtattct tgtggtaaat aagctttatt tgcatcaact atacccaaat tgccaccatt
      421 ttctttcgtg ccaatatcat ctgaggactc ctgtgcggca gtggcaaaat ccttacctgc
      481 agagatctct gctaatactg tatctgcctt ggcccgtaca gatggatcaa gtgcttgtac
      541 aaccttggca ttaagtaatt gttgcttaat actacgtcta aaatcagcaa tactccaatc
      601 ataattagag cgtaagactt cttcgaatac ttgctgctca ctgcctagta gcccaagttt
      661 tttaagagta tcaatctgag agttgacttc atcttcgctg acagtaatac ctttttctct
      721 tgctatcttt tcggcgtacg tattattaat gataagatcg cgtatttttt tcttttcttc
      781 ttgtaattgc tgggcacctt gttcactggt aaagtctacc ccaccttttt cttcaaaaaa
      841 gtgaatcaga tggcgaagtt caaataaata ttcctcatac ggaacatacg taccatcaac
      901 ttttgcaaat ggtagcggta ccactttagt aatctgatag gcaaatgcag aagtagattg
      961 aaatttatac aaactaagaa tggtgtacgt ggcaaatcct acaataagag caaccaaaat
     1021 actaacagat actataacaa ttcgatgccg cgaatgctgc aaggggtaaa tgtatttttt
     1081 aac
//
LOCUS       DAOPLS010000277         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_165780_length_1082_cov_2.718598, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000277 DAOPLS010000000
VERSION     DAOPLS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165780_length_1082_cov_2.718598"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PKV52_04790"
     CDS             complement(<1..1000)
                     /locus_tag="PKV52_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPD99560.1"
                     /translation="MSLSLGLGQGVKNYITSQLGQYDNVNLYVVSRQGANDFSSGFGN
                     GDPKAYDPNKTQGGDFSQIFLTESDIAKIQSAEGVSTAQVPYMPSFNYVTSVDGNKYQ
                     VVSEPIVPQLPLTVVAGTKINLDDVGSIMLSRKFVSLTGASSSEAAIGKSLSFTYQTV
                     NDETVTEDFIIKGIYEPTLIDASVKVSQTDAQRIATVQAFGGVPQVANVYISKSDTVT
                     DEQLKENLKNVGFSVTSLADVNNTLNGVVTGVQLALAAFSAVAILASIVGVINTLFMA
                     VLERTGEIGLFRALGAKPKTIFALFSVEAALLGFWGSIFGLAFAYGAQYAINTIAEN"
BASE COUNT          301 a          248 c          212 g          321 t
ORIGIN      
        1 aattttcggc gatagtattg attgcatact gtgcaccgta ggcaaaagca agtccaaata
       61 tactgcccca aaaaccaagt agagctgctt ctacactaaa gagtgcaaat atagtctttg
      121 gcttggcgcc aagagctcta aataagccta tttctccggt acgctctagc acagccataa
      181 acaaggtgtt tataacacca acaatggatg ctagtattgc caccgcgcta aacgcagcga
      241 gcgctaattg aacaccagtt actacaccgt taagtgtgtt attaacatct gctagactcg
      301 tcacagaaaa gccaacattt tttaaatttt ctttgagttg ctcgtctgtt acggtatctg
      361 attttgatat atacacatta gctacttgtg gtacaccgcc aaaagcttgc accgtagcta
      421 tacgctgtgc gtctgtctgg cttaccttaa ctgacgcatc aatgagcgtc ggttcgtaga
      481 tacccttgat aataaaatcc tctgtaacgg tttcgtcatt taccgtctgg taggtaaacg
      541 ataagctttt tcctatggca gcttcacttg agcttgcacc agttaacgaa acaaacttac
      601 gcgataacat aatactgcca acgtcatcta aatttatttt ggttcctgct accaccgtaa
      661 gtggtagttg tggcacaata ggctcgctga caacctggta cttattgcca tcaacagacg
      721 ttacatagtt aaaactaggc atgtatggga cttgtgctgt acttactcct tctgcagatt
      781 gtatctttgc aatatcgctc tctgttaaaa aaatttggct aaagtcacca ccttgcgttt
      841 tgttggggtc gtaggctttt ggatcaccat taccaaaacc actactaaaa tcgtttgccc
      901 cttggcgtga cacaacatac aaatttacgt tatcgtactg gcctaattgt gaagtaatgt
      961 agttctttac cccttggcca aggccgaggc ttaatgacaa cgtaaatgta ccaatgccta
     1021 tcgcaagtag cgtcaaaaat gttcgcagct tagaccgtcg taagttaccg tttgcagtgg
     1081 tt
//
LOCUS       DAOPLS010000278         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_166113_length_1081_cov_1.984405, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000278 DAOPLS010000000
VERSION     DAOPLS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166113_length_1081_cov_1.984405"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..854)
                     /locus_tag="PKV52_04795"
     CDS             complement(15..854)
                     /locus_tag="PKV52_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99561.1"
                     /translation="MKHKKLKLNTLQKVMTYLDIDLPRLSDSGATPAETYAYTRKLLE
                     KLGNPEHGISTVHVAGTIGKGTVSYLIDAMLRAHDQRTALCVSPHVYDARERIQINGQ
                     LAPERQFVRLVNTVVGAAHEIVNDHPINYFGILMAISLLGASQQRLDYVIVEAGLGGK
                     YDQTNLIEDSKKTAVIAQIGNDKLSEQGVLAEARDTAVTITDDDLVVALKQDAQAEAV
                     IRSTAKEHDAQLNWVQRTGVYQNDDFDLACEAARQIANRDGWHFDEERARLAADMTYI
                     PGR"
BASE COUNT          246 a          273 c          277 g          285 t
ORIGIN      
        1 aagaacgtgc acgcttagcg gccagggata taggtcatgt cggcggctaa gcgtgcacgt
       61 tcttcatcaa agtgccagcc atcacggtta gcgatctgtc gagcggcctc acaagctaaa
      121 tcgaagtcgt cattctggta gacgccagtt ctctgcaccc agttcagttg ggcatcgtgc
      181 tcctttgcgg tagatcgtat aactgcttcg gcctgggcat cctgtttaag cgcgaccacc
      241 aggtcgtcat cggttattgt cacggcagta tcccttgcct cggccaaaac gccctgctcg
      301 gaaagtttgt cgttgccgat ttgggcaatc acagccgtct ttttgctgtc ttcgatgagg
      361 tttgtctggt catattttcc gccaagacct gcttctacaa tcacgtagtc aagacgctgc
      421 tgactggcgc cgagcaggct aatcgccatg agtatcccga agtagtttat gggatgatca
      481 ttgacgattt cgtgagctgc accaactacc gtatttacaa ggcgaacaaa ttgccgctca
      541 ggagcaagtt gtccattgat ctgaattcgc tcccttgcat catacacgtg tggactaaca
      601 caaagcgcag tacgctggtc atgggcacgc agcatggcat cgatcaaata actaacggta
      661 cccttaccaa tcgtaccagc aacatgcacg gtgctgatgc cgtgctcggg gttgcctagt
      721 ttttctaata gcttgcgggt atacgcgtac gtttcggcgg gagttgcccc cgaatccgaa
      781 agtcgcggga ggtcgatatc gaggtaggtc ataacttttt gcagcgtgtt aagtttgagt
      841 tttttatgtt tcatggctgt cggtagtata ccattttcgc cataactaca gatgggctcg
      901 ggcttagcgc tcttgacagt agagtgctaa aaagtggtac aatgattgca atatgaccga
      961 tcgacaagcc aagctcttgg ctacaattat tgaacaatac gctgaggtcg ccgccccagt
     1021 tggcagcgtg ttgctcgcca agctattcaa cgtctcaagc gcaactatcc gtaatgagat
     1081 g
//
LOCUS       DAOPLS010000279         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_166180_length_1080_cov_4.474146, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000279 DAOPLS010000000
VERSION     DAOPLS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166180_length_1080_cov_4.474146"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..871
                     /locus_tag="PKV52_04800"
     CDS             230..871
                     /locus_tag="PKV52_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HPD99562.1"
                     /translation="MKEVIFALLAGGLGVLLLTAGYRFARILIPLWAFFAGFSVGAAV
                     FADATSVGFIATTMGIVTGLIIGFVFALFAYFFYSLAVVLLGATTGYWIGTAFMSWIG
                     FSKGFLSAVVGISLGVVFALATIGLNVAKYLLVALTSMAGAVALVGGILVLFNKIELS
                     AFDYTTASAVISNSVLWTIVTFVLISLGIVIQIITTNSYVLDDWTTEYGAPKK"
     gene            872..1078
                     /locus_tag="PKV52_04805"
     CDS             872..1078
                     /locus_tag="PKV52_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPD99563.1"
                     /translation="MFVSAAQAGAMLRGGAVGVVPTESVYGLIALATDVAAIERVYDV
                     KHRPSTKPCIVLIAATSVARAMSP"
BASE COUNT          279 a          213 c          254 g          334 t
ORIGIN      
        1 atgacgtaca gctgatacat agttgtcatt atacagcttc ttgtccgtca gctgacggac
       61 tactcccgaa tgctctttgt tttacccagg aatatatata aaaggtacaa gcaagttacc
      121 ttgcaactag agtagtataa attgtttgct ccctattggt caataaaatt acaatggtca
      181 aaacaactag taacagcgta caatataaat acaactaagg agactacgta tgaaagaggt
      241 aatatttgcg ctactggcag gtggtttagg cgttttgcta ctaacagccg ggtatcgttt
      301 tgctagaatt ctaattccac tttgggcatt ttttgccgga ttttcagttg gagcagccgt
      361 ttttgcagat gccacgagtg ttggctttat tgcgacaaca atgggcatcg tcacagggct
      421 catcataggt tttgtatttg cactatttgc ctactttttc tacagcttgg ctgttgtact
      481 acttggcgca acaaccggtt attggatagg tacggcattt atgagttgga ttggttttag
      541 taaaggtttc ttaagtgccg ttgttggtat atcacttggc gttgtatttg cgctcgcaac
      601 tattgggctc aatgttgcaa aatatttgct cgtggcactt acatctatgg ctggtgcagt
      661 tgcgctggtt ggtggcatat tggtgctatt caataaaatt gaactcagcg catttgatta
      721 caccacagcg agtgcggtta ttagcaactc ggtactatgg acgatagtta cgtttgtatt
      781 aatatcactg ggtattgtta tacaaataat taccacaaac tcgtacgtat tagatgattg
      841 gacaaccgaa tacggagcgc caaagaaata gatgttcgta tctgccgcac aagctggtgc
      901 aatgctgcgc ggtggcgctg ttggtgtagt gcccacagaa tcggtctacg ggctcattgc
      961 cctcgctact gacgtagctg ctattgagcg tgtgtacgat gtaaaacatc gcccaagcac
     1021 taaaccatgt attgtcttaa ttgcagctac gtcagtagcg agggcaatga gcccgtagac
//
LOCUS       DAOPLS010000280         1074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_167732_length_1074_cov_2.333660, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000280 DAOPLS010000000
VERSION     DAOPLS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167732_length_1074_cov_2.333660"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKV52_04810"
     CDS             complement(<1..237)
                     /locus_tag="PKV52_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99564.1"
                     /translation="MDIYELEKVAIVILALIMAYAEFRQAIILKGSWVKCGLGVVGIY
                     WAVYYGYSLYRTYFDLRLPSHQVFVRSGILITLAF"
     gene            complement(218..>1074)
                     /locus_tag="PKV52_04815"
     CDS             complement(218..>1074)
                     /locus_tag="PKV52_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99565.1"
                     /translation="YQLRNVDIALFDHAINRTDPEKGRNLIHRFYREGATIVLYPHAA
                     TGAWWMDSDIFPKERAVQHELVIAEGQERVSKIINPDMRVHKIGWSYCPIREFQKPKQ
                     LKRILFAPIHGPMKGDLREECVAANKRVYETLLDMVDKYQIVIRHLNPLEKIGLWYSP
                     KPVFVLGKPDGSYKDIDAADLVIAEGTFMYLAVARGKPTIGINQHVPIRPNNTNKEFA
                     PKRWDEYGEYMGYPIDFDDGDIISLIDRAMTEQTTWKNLFIGKSMDAKNLSNILKDIR
                     SQDGHIRA"
BASE COUNT          306 a          235 c          176 g          357 t
ORIGIN      
        1 aaaggctaag gttattaaaa tacctgacct tacaaacact tgatgacttg gcaagcgtaa
       61 atcaaaataa gtgcgataaa gtgaatagcc gtaatagacc gcccaatata tgcctactac
      121 gcctaatccg cattttaccc acgacccttt taatatgatt gcctgtctga attctgcata
      181 cgccatgata agcgcaagta tgacaatggc tactttttca agctcgtata tgtccatctt
      241 gactcctaat atctttcaga atattagaca aattttttgc gtccatagac ttacctataa
      301 atagattttt ccaggtggtt tgttctgtca tggctctatc tattaatgat attatatcac
      361 catcgtcgaa atcgataggg tatcccatat actcaccata ctcgtcccaa cgttttgggg
      421 caaattcttt attagtatta ttcgggcgta ttggcacgtg ctggtttatg cctatagtag
      481 gcttacctct agcaacggct agatacataa aagtaccctc tgcaattact aaatccgctg
      541 catctatatc tttatacgaa ccatcaggtt tacctaatac gaacactggt tttgggctat
      601 accaaagacc aatcttttct aaaggattta gatgccttat tactatttga tatttatcta
      661 ccatatctaa aagtgtttcg taaactcttt tatttgcagc aacgcactct tctctaagat
      721 cacctttcat tgggccgtga attggagcga ataaaattcg tttcagctgc tttggttttt
      781 gaaactctct aatcgggcag taagaccatc caattttatg cacacgcata tctggattta
      841 ttattttaga cacgcgctct tgaccttcag caataactaa ttcatgttgt accgcccttt
      901 ctttaggaaa tatatcgctg tccatccacc aagcaccagt tgcagcgtgc gggtaaagaa
      961 ctatagttgc cccttctcga taaaacctat gaataagatt tctacctttt tcagggtcag
     1021 ttctatttat tgcgtggtca aacaatgcaa tatctacgtt tcgtaattga tact
//
LOCUS       DAOPLS010000281         1065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_169810_length_1065_cov_3.921782, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000281 DAOPLS010000000
VERSION     DAOPLS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169810_length_1065_cov_3.921782"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1065)
                     /locus_tag="PKV52_04820"
     CDS             complement(<1..>1065)
                     /locus_tag="PKV52_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD99566.1"
                     /translation="AVQSLKDSNKRVTALPIVFDENAAKTFVKQLPFTLTDDQRIVAW
                     KILQDMQQRQPMNRLVEGDVGSGKTVVAALASLMALQSNMQVALIAPTEILARQHYKT
                     LRTLLQHTKHAQDIQLLVGSVNKTEKQQIKHKISTKKATLLIGTHALLERDIDWHTLG
                     LVIIDEQHRFGVKQRQRLLQAAGHMPHTLCMTATPIPRSLALTVYGELDISIIKQKPL
                     HKASITTSIVSPNSTQQMYSKITASLEAGRQAYIVCPLIHDSDVLDVASAEETFKEVT
                     QKYFKKYRVGLLHGRLKPVQKDEVMQAFAKHELDVIVTTTVIEVGVDVPNATDMVILG
                     ADRFGLAQLHQLRGRIGRGV"
BASE COUNT          262 a          239 c          233 g          331 t
ORIGIN      
        1 gtaccccgcg accaatgcgc ccgcgtagct ggtgcaactg cgctaaacca aacctatcgg
       61 ccccaagaat aaccatatcc gtagcattag gtacatctac gccaacttct ataactgtag
      121 ttgtcacaat gacatctagt tcgtgtttag caaacgcttg cattacttca tctttttgga
      181 cgggctttaa acgaccgtgc aacagtccaa cccggtattt tttaaaatat ttttgggtca
      241 cttctttaaa ggtttcttca gcactggcca catccagtac atcgctatca tgtattaacg
      301 gacaaactat gtaggcttgg cgaccggctt ctaaagacgc agtaatttta ctatacattt
      361 gctgagtaga atttggtgac acaatacttg tggtaattga tgctttatgc agcggttttt
      421 gcttaataat acttatatcg agctcaccgt acacagtgag cgcgaggctt cgtggaattg
      481 gtgtagcggt catacatagc gtatgtggca tatgacctgc tgcttgcagt aagcgctgcc
      541 gttgcttgac gccaaaacgg tgttgctcgt ctatgataac tagtccgagt gtgtgccaat
      601 ctatatcacg ctctagtagt gcatgtgttc ctataagtag tgtcgctttt ttggtgctta
      661 ttttatgttt aatctgctgt ttttcggttt tattgacaga cccaacgagg agttgaatat
      721 cctgtgcatg tttggtgtgc tgcaggagcg tacgcagcgt tttatagtgc tggcgtgcca
      781 atatttcggt tggcgcaata agcgctacct gcatattact ttgtaacgcc ataagtgatg
      841 caagtgccgc aacgactgtc ttaccagacc ctacatcgcc ttcaactaaa cggttcattg
      901 gttgacgctg ttgcatatct tgcagaatct tccaagcaac aatccgctga tcatctgtta
      961 aggtaaatgg caattgtttc acaaacgttt tagcagcgtt ttcatcaaat acaataggca
     1021 aagccgtgac tcgtttgtta ctatctttga gcgattgcac agcgc
//
LOCUS       DAOPLS010000282         1065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_169830_length_1065_cov_3.479208, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000282 DAOPLS010000000
VERSION     DAOPLS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169830_length_1065_cov_3.479208"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1065)
                     /locus_tag="PKV52_04825"
     CDS             complement(<1..>1065)
                     /locus_tag="PKV52_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99567.1"
                     /translation="VTLKPKYGLKEPQQSENRLIYPLNNNAKKVFTLGAASIKEDILL
                     EKYIRDDMVFDYTLEMPDYLEAKLETDGSISIYGPANSALIGDVSASTESDSQLLDKA
                     RQKSSKNRLIFKLPAPYILEVGKTMSKATAEFELKDNIISLKASNLKAANYPITIDPS
                     IYVETAAKLMKGNNETNVDFDVDNELIQKSQTTGARIDSWQSNLDISQEVWGHSTAAA
                     GGYIYRAGGKTEDGSIVTPPVAASSVDSLQSSNSTSFTMSMPGTRPAGDLYVALMCHD
                     GTGAVTPPAGGGWTEFADIGEFAGYYKVGTNEGGGDEAASYTWTGPSEKWGGAILRVT
                     NFNTSNPVSPTPTTASSGSN"
BASE COUNT          281 a          245 c          195 g          344 t
ORIGIN      
        1 tgttagagcc agatgaagct gtagtaggtg ttggcgacac aggattactc gtgttaaaat
       61 tagtaaccct aagaatagct ccaccccact tttcgctcgg tccggtccac gtatagctag
      121 cggcttcatc acctccacct tcgtttgtgc ctactttata gtaaccagca aattctccta
      181 tgtcggcaaa ctctgtccag cccccaccag caggcggagt aactgcaccc gtgccatcat
      241 gacacatcaa tgctacgtat aaatcaccag ccggtctcgt acccggcata gacattgtga
      301 aagaagtaga gttggagctc tgtaaactat ctaccgagct agcagcaacg ggtggcgtta
      361 caatactacc atcttctgtt ttaccacccg ctctataaat atatccgcca gctgcagccg
      421 tactatgacc ccaaacttct tggcttatat ctagatttga ttgccacgaa tcaatcctag
      481 caccagtagt ctggcttttt tgtataagtt cattatcaac atcaaaatct acgttcgttt
      541 cattattgcc cttcatgagt ttggctgctg tttcaacgta aatactaggg tctatcgtaa
      601 ttgggtaatt tgctgctttt aaattgctag ctttcagtga aataatattg tctttaagct
      661 caaattcagc cgtagcttta ctcatcgtct taccgacttc taatatatac ggggcaggca
      721 gtttaaaaat aagacgattt ttagatgatt tttggcgagc tttatctagt agctgactat
      781 cactttctgt actcgcgctt acatcaccaa tcaatgcaga attagcaggt ccgtatatac
      841 taatagaacc atctgtttct agttttgctt ctaagtaatc gggcatttcc aaagtgtagt
      901 caaataccat atcgtctctg atgtattttt ctaataaaat atcttctttt atagacgccg
      961 cgcccaacgt aaatactttt ttagcattat tattaagcgg atatattaaa cgattttccg
     1021 actgctgggg ttcttttaga ccgtattttg gctttaaggt tactg
//
LOCUS       DAOPLS010000283         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_170548_length_1062_cov_4.356504, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000283 DAOPLS010000000
VERSION     DAOPLS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170548_length_1062_cov_4.356504"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKV52_04830"
     CDS             complement(<1..699)
                     /locus_tag="PKV52_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD99568.1"
                     /translation="MSTESIGNLDLPVGHPNHQESEQSVAEILLVDSNEVTPEEAMFW
                     RDAGLIFLKNLSKESFRLRFGSPKPEEDTGLIPVVDYFVFSQQHHHKMLLLMHEAKLI
                     GACSIFDYPLRDSMGSSTVINTDDVVELAVTISDEQQGKKLGTVLLEQAVQVAGADGK
                     THVYTSFNSDNDRSRRLVERILGSANIVAGKDNLQDKFWRIPGVPADDYMIQKTLCVS
                     IVVMNLQSLMILSEG"
     gene            complement(770..>1062)
                     /locus_tag="PKV52_04835"
     CDS             complement(770..>1062)
                     /locus_tag="PKV52_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99569.1"
                     /translation="KDSSNSKEMQFSLCANFKTENKSSESSPLPTALMGQSLSPQSYS
                     SVDYKNHPAGYHCFEKETISSANSYDSYFESLYNNQSPQDTTQPMIDPYNFN"
BASE COUNT          294 a          212 c          190 g          366 t
ORIGIN      
        1 tccctcgctt agtatcatca agctctgtag attcatcacc acaatagaga cgcatagcgt
       61 tttctggatc atatagtcat cagctggtac tcctggtatt ctccagaatt tatcttgtaa
      121 attatcttta cctgcaacaa tattagcaga gcctaaaata cgttcaacta aacgacggct
      181 cctatcgtta tctgagttaa aacttgtata aacgtgggtc ttgccgtctg caccagcaac
      241 ttgtaccgcc tgttctaata gcactgttcc taatttttta ccttgctgtt catcacttat
      301 ggtgaccgcc agctctacaa catcatcagt attaataaca gtagaagagc ccattgaatc
      361 tcgcaatgga taatcaaata tactacatgc acctattagc ttagcttcgt gcatcaataa
      421 caacatctta tggtgatgtt gttggctgaa tacaaagtaa tcaacaactg gtataagacc
      481 tgtatcttct tctggtttgg ggctaccaaa gcgtagacga aacgattctt tgctcaagtt
      541 ttttaaaaat atcaacccag catcacgcca aaacattgcc tcctctggtg tgacttcatt
      601 gctatctacc agtagaatct ctgctacaga ttgctctgat tcttgatgat ttggatgtcc
      661 aactggaaga tcaagattgc ctatagactc tgtactcatt aaaaatatta tataatactt
      721 ttaatatttt tactatactt tgtgatagta gtacttgatg tatttctcgt taattaaaat
      781 tatatggatc aatcataggt tgtgtggtgt cttgtggtga ttgattattg tataaacttt
      841 caaaatacga atcgtagctg tttgcgcttg agatcgtttc tttttcaaaa caatggtacc
      901 ctgcagggtg gtttttatag tccactgaac tatagctttg cgggcttagg ctctggccca
      961 taagggcagt cggtaagggg ctagattctg atgatttatt ttcggtttta aagtttgcac
     1021 acaagctaaa ttgcatctct ttagagttac tactgtcttt tt
//
LOCUS       DAOPLS010000284         1060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_171074_length_1060_cov_3.310448, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000284 DAOPLS010000000
VERSION     DAOPLS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171074_length_1060_cov_3.310448"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..658
                     /gene="orn"
                     /locus_tag="PKV52_04840"
     CDS             128..658
                     /gene="orn"
                     /locus_tag="PKV52_04840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HPD99570.1"
                     /translation="MNATKNDIPQKLLWLDLEMTGLIPATDRILEIAAIVTDFNFTEL
                     DRYESVVYQPPEVLARMNEWSRSTHTASGLLDRVNVAPNEQRVVADFLEFVLRNFGNE
                     PVVLAGNSIHQDRRFIRQWWPDIEARLHYRMLDVSSFKIVIQGKYGKVFNKKETHRAL
                     DDIKESIAELQYYLSL"
     gene            804..>1060
                     /locus_tag="PKV52_04845"
     CDS             804..>1060
                     /locus_tag="PKV52_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99571.1"
                     /translation="MPTTVINPESKSDFKHSRFTQEVHSYLEGLNDPLATSIQDLLER
                     QIELNDLFDCLVNKEENDTFLDYWQNQYGQNIDGEKGSRSWT"
BASE COUNT          373 a          227 c          204 g          256 t
ORIGIN      
        1 taaccagaag gcacgggcga gaccgcacaa acggctttct aggtgggccg attcaaacca
       61 cctaaatcgt cccgcgtgtg gccacaagcc ccgcgggatt ttcctatatg tgcaataata
      121 gatagcagtg aacgctacaa aaaacgacat accacaaaaa ctactttggt tagatcttga
      181 aatgacaggt ctaatacccg caacagaccg tattctagaa attgctgcca tagtaacaga
      241 ttttaatttt acagaactag ataggtacga atcagtggtg tatcaaccac cagaagtctt
      301 agcacgcatg aacgaatggt cacgcagcac gcacaccgct agtggcttgc tagacagggt
      361 aaacgtcgcc ccaaacgaac agcgcgttgt agcagatttt ctagagtttg tgctccgcaa
      421 ttttggcaat gaaccagtag tgctggcagg caatagcatt catcaagaca ggcgctttat
      481 ccgccaatgg tggccagata tagaagcgcg tttgcactac cgtatgctag atgtaagtag
      541 ctttaaaatt gttattcaag gcaaatatgg caaagtattc aataaaaaag aaacccaccg
      601 cgcactagat gacatcaaag aatccattgc cgaactccaa tattacttga gcctgtaatt
      661 gcttagtaac ctaagcctaa ttcatcactc aacagatcaa accctgttag cttttaccat
      721 aaaaaaacaa acaactacat tgacaaaaaa gcaaaactgt gctaataata tatatgtaat
      781 taactaaaac aaaaacatat acaatgccga caacagtaat aaaccctgaa tctaaaagtg
      841 acttcaagca tagtcgtttt acgcaagaag tacatagtta tcttgaggga ttaaatgacc
      901 ctcttgctac gtcaatacag gatttactag agagacaaat tgaactcaac gacctatttg
      961 attgtttagt aaacaaagaa gaaaatgata catttcttga ctattggcaa aatcaatatg
     1021 gtcaaaacat tgacggagaa aaggggtctc gttcgtggac
//
LOCUS       DAOPLS010000285         1060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_171213_length_1060_cov_1.930348, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000285 DAOPLS010000000
VERSION     DAOPLS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171213_length_1060_cov_1.930348"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKV52_04850"
     CDS             complement(<1..502)
                     /locus_tag="PKV52_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99572.1"
                     /translation="MRSKSKVIGIVGAIGIFVGLLTTINTQTYTTYLPIIAKKNCNAG
                     VSMAWSPGNLVQKSKSDFCAQRYFNSGTWALNDPSGIPVMRPGNFDTILAYLIENNYC
                     GDVILFNEPDGQDNIDIWTARAYYIKLVNTIPCVRVLTPNAIDRNYGINFSQLLVLVG
                     DNQMYSG"
     gene            complement(492..740)
                     /locus_tag="PKV52_04855"
     CDS             complement(492..740)
                     /locus_tag="PKV52_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99573.1"
                     /translation="MPDAQDTAKGTNNKMVIKANTTDGGIIATINVNRWGNNKITGYI
                     CFTNGDEEVIDESLIKKLMRNPENKKHVKILRGILDAL"
     gene            complement(847..1017)
                     /locus_tag="PKV52_04860"
     CDS             complement(847..1017)
                     /locus_tag="PKV52_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99574.1"
                     /translation="MTVAQFMELAEIYWRFVLDLKAYIMLAYPTLTDTEAEYYAKQML
                     RKAAIDTDARID"
BASE COUNT          287 a          221 c          196 g          356 t
ORIGIN      
        1 tacccgaata catctgattg tcgccaacca aaaccaacaa ttgactaaaa ttgataccat
       61 agttgcgatc tatggcgttt ggcgtaagca cccgcacaca aggtattgta tttacaagtt
      121 ttatatagta tgcccgtgct gtccatatgt cgatgttatc ttgtccatct ggttcgttaa
      181 acaatatcac atcaccgcaa tagttatttt caattaagta tgccaggatt gtatcgaaat
      241 taccagggcg cattacaggt ataccgcttg gatcattcaa tgcccatgta ccagaattaa
      301 aatatcgttg ggcacaaaag tcactttttg atttttgcac cagattgccg ggcgaccacg
      361 ccattgatac acccgcatta caatttttct tagctataat tggtaaatat gtagtgtagg
      421 tttgcgtgtt aatggttgtt aataagccta caaagatgcc gattgcgcca acaataccta
      481 tgactttaga tttagagcgc atctaagata cctcttagaa tcttaacatg ttttttgttt
      541 tcggggtttc gcatgagttt tttaattaac gattcatcaa tgacttcttc atcaccgtta
      601 gtgaaacaaa tatagcccgt tatcttattg ttgccccaac ggttaacgtt gatagtagca
      661 ataatgccac catctgttgt gtttgctttt ataaccattt tgttgtttgt accctttgcc
      721 gtatcttgcg cgtccggcac gcaaggtggc tgtatcaaca ccgcgagggg tttcgcctcg
      781 ccgcatcttt tgtaaatcac taaaatctgc atttttcagg attgtatttg tacccatatt
      841 tatattttaa tctatacgcg catctgtgtc aattgcggct tttcgtagca tctgtttagc
      901 ataatattca gcttcggtat cggttagggt tggatatgcc aacatgatat acgctttcaa
      961 atcgagcacg aaacgccaat atatttctgc caattccata aattgtgcaa cagtcattgt
     1021 agtagtttat cacaccgcaa cgtaaaatat tagtatggct
//
LOCUS       DAOPLS010000286         1060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_171249_length_1060_cov_1.703483, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000286 DAOPLS010000000
VERSION     DAOPLS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171249_length_1060_cov_1.703483"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1060)
                     /locus_tag="PKV52_04865"
     CDS             complement(<1..>1060)
                     /locus_tag="PKV52_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HPD99575.1"
                     /translation="SVTTNSKFTIHDYELALRNGGAAAAAFGVSLDDFNVVVAATADE
                     LGTGMRDGTSFANMLYRLTPNSDKAKDAMQELGWVTASGSNIFYDAEGNLKGVNDIAQ
                     ILYNTLEGESKIMVEVGGRTAEQNKLLGELRAEYDAAQETVADYTTGVKGMLASEDEK
                     NKALEEAQNIINITGPKIDELVAIQGDYVESTKQLTSEQKLLAIETIFGRDALGAVLG
                     VMKEGAPIVDDVARVMDALAVSEEEAFALVSSGITEYELLQQTMERTDAVENAKTRMD
                     NLHGSMEILSGIIEALSIEVGDQFLPILDKIVKAFTDWLNNSQPLQEQLINIADAVAG
                     FVEALFNGEDPIDSFSKM"
BASE COUNT          290 a          244 c          188 g          338 t
ORIGIN      
        1 aacattttac taaacgaatc aatagggtct tctccattaa ataatgcttc tacaaaaccc
       61 gcaacagcat cagcgatatt tataagttgt tcttgtaatg gttggctatt atttaaccaa
      121 tcagtaaatg ctttaacgat cttatctagt ataggcagga attgatcacc aacttcaata
      181 cttaacgctt caataatacc agacagaatt tccatagaac cgtgcaggtt atccattctg
      241 gttttagcat tttctactgc atctgtgcgt tccattgttt gttgcagcaa ttcatattct
      301 gtgataccgg aagataccag ggcaaaggct tcttcttcac ttacggccaa agcgtccata
      361 acgcgggcta cgtcatccac aataggagcg ccttctttca tgacacctaa taccgcgcct
      421 aaagcatcgc gtccaaagat cgtttcgata gccaatagtt tttgttctga ggtaagctgt
      481 tttgtgcttt caacataatc gccttgaata gccaccagtt catctatttt tggccctgta
      541 atatttataa tgttctgtgc ttcttctaat gctttgtttt tttcatcctc agatgcaagc
      601 attcctttta cgccagtcgt ataatcagca acggtttctt gtgccgcgtc atattctgcg
      661 cgtagttcac ctaaaagttt gttttgttca gcagttctac cgcctacctc caccatgatc
      721 ttagattcgc cttctaacgt attatagaga atttgggcaa tgtcgtttac gccttttaga
      781 ttaccctctg catcatagaa tatattgctt ccgctggcag taacccaacc tagttcttgc
      841 atagcatctt tagctttatc tgagttagga gttagacgat agagcatgtt agcgaaagaa
      901 gtaccatcac gcatacctgt acccaattca tcagcagttg ccgcaactac aacgttaaaa
      961 tcatcaagag acacgccaaa agcagcagca gcagcaccac catttcttaa ggctaattca
     1021 taatcgtgaa tcgtgaattt agagtttgtg gtaacagagg
//
LOCUS       DAOPLS010000287         1053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_173051_length_1053_cov_1.566132, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000287 DAOPLS010000000
VERSION     DAOPLS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173051_length_1053_cov_1.566132"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKV52_04870"
     CDS             <1..445
                     /locus_tag="PKV52_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPD99576.1"
                     /translation="NEVADDLDNGDPWYRHEFDLIQRTDKPILGICLGLQMIAVALGG
                     SLSRLPSIVDTNKHVVLTNEGEAYFSQQELYVHERHQWVVDSADTVGMNILAHSNDGI
                     EILHYPERNIIGTQFHPEIDASPNSPKLFWDILQVIEQPAEVARA"
BASE COUNT          361 a          195 c          211 g          286 t
ORIGIN      
        1 aaatgaggta gccgacgatc tagataacgg cgatccttgg taccggcatg aatttgatct
       61 catacagcgt accgacaagc ctatcttagg gatatgcctg ggactccaaa tgattgcagt
      121 ggcccttggt ggttcactaa gtaggttacc atctatcgta gacaccaata agcacgtagt
      181 acttactaat gaaggtgaag catattttag tcagcaagaa ctgtatgtac acgaacgaca
      241 ccaatgggtt gtggactctg cagataccgt tggcatgaac attcttgcac acagtaacga
      301 cggcatagag atactgcatt atcctgagcg taatattatc ggcactcaat ttcacccaga
      361 gatagatgct tcacccaatt cacccaaatt attttgggat attctacagg taattgaaca
      421 accagcagag gtagcacgtg cgtgacgcca aacagcaagg ctttgtaaca atgattgtgc
      481 tattgttggt cgtgctcgtt atagtgataa ccatagcatt tatgcgtgtt ttaaaagcaa
      541 atagttaaca gaaaaataat atagcaataa aagcaaaaac ctgctagaat attaatatgc
      601 ctaaaaagtt aatacggcta ggatgcaaag aatctcgcaa ccataaagtt agtgcagggc
      661 tgccttttta attacttgaa acactatgtg gcaatgtctc aaatcgtgac acgcaaaaac
      721 taaataacga ttcttacata ccttgtgttc atattttgaa ggtgcactaa cacctatgtc
      781 aaggattaaa actaacggca atccaatcgc attattatac aataatgttc ttgcgtattc
      841 tgatggtaaa cgcagagact caattgttta ccactagggt tacggttaat gatttgtggt
      901 tgcaatataa aacaaaatac gagccagcta gtactagata aaagggaaat aaatataatc
      961 ggttttgcgt aaaaagtgaa ggctgtaaca gttagctact aggcatagac tatagtatat
     1021 gaaaaagcat tataataaca acatgataga atc
//
LOCUS       DAOPLS010000288         1041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_175954_length_1041_cov_3.252535, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000288 DAOPLS010000000
VERSION     DAOPLS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175954_length_1041_cov_3.252535"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..964)
                     /locus_tag="PKV52_04875"
     CDS             complement(68..964)
                     /locus_tag="PKV52_04875"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPD99577.1"
                     /translation="MRQKTFQIIGVIGVIISIVIFLKNPSFLTPDKLLVFLVFVFMIF
                     KQAIEMLKRFLPFVAILLVYESFRGIADGLNSRVEYMWMPAVDRLLFGGTLPTTTLQN
                     WWWMGQTNWYDFLFYLPYMLHFVLPIALAVYVWKRIPSKYWQLVLSYVLVSFIGFFVF
                     LGFPAAPPWMASDLGYIEPIHRISSDVWWSLGVKDFPSFYNEISPNPVAAVPSLHAAY
                     ATLFALFITYLFKSKWRFISWIYPALIYIGTVYLGEHYFIDELLGAMLAIAVFYATPH
                     IAKTLQHAAKKLQSKYRHRTPK"
BASE COUNT          356 a          192 c          192 g          301 t
ORIGIN      
        1 actatgtaag atctttacta catagattaa aaaagtacaa aatatcccat acgtatacat
       61 aggatgttta ttttggcgtt ctatgtcggt acttactttg aagtttttta gcagcatgct
      121 gtagtgtctt tgctatgtgt ggtgtggcat aaaaaacggc tatagcgagc attgcaccaa
      181 gtagctcgtc tataaaataa tgttcaccca agtacacagt acctatatat ataagcgcgg
      241 ggtatatcca actaataaat cgccacttac ttttaaatag gtacgttatg aacaatgcaa
      301 ataatgttgc ataggcggca tggagtgatg gcacagcggc aactgggttt ggtgaaattt
      361 cattataaaa tgatgggaaa tctttgacac ctaagctcca ccacacgtcg ctagatattc
      421 tatgaattgg ttctatgtaa cctaggtcac ttgccatcca tggtggagca gccggaaaac
      481 ccaagaatac gaaaaagccg ataaatgaaa cgagtacata agacaaaact aattgccaat
      541 acttgcttgg tatacgtttc caaacataca cagcaagtgc tataggtaat acaaaatgta
      601 gcatatacgg taagtaaaat aagaaatcat accagttagt ttggcccatc caccaccagt
      661 tctgcagagt agtggttggt aatgtaccgc caaacaataa tctatctacg gcaggcatcc
      721 acatgtattc aacgcggcta tttaaaccat cggcaatacc gcgaaaagat tcgtatacga
      781 gtaaaattgc aacaaatggc aaaaagcgtt ttagcatttc tatagcttgt ttaaatatca
      841 tgaacacaaa cactaaaaac accagtagtt tatctggtgt caaaaaactt ggatttttta
      901 gaaaaattac gatagatatg attacaccta tcacaccaat tatttggaac gttttttgtc
      961 tcattgacta atggtatctt ataatggcga tgctatctat agcgaaagac ttattcgcta
     1021 tatgatacat tagtactatg a
//
LOCUS       DAOPLS010000289         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_177497_length_1035_cov_3.072449, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000289 DAOPLS010000000
VERSION     DAOPLS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177497_length_1035_cov_3.072449"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>1035
                     /locus_tag="PKV52_04880"
     CDS             149..>1035
                     /locus_tag="PKV52_04880"
                     /inference="COORDINATES: protein motif:HMM:NF027609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HPD99578.1"
                     /translation="MLYGNEIRRHYYLPKTVIVAPRRSARPTSFTESITVANQAACVL
                     CNSHEECMYSVQSSDGWVIKSVRNPYGALTEHQKHAYGYQEVIIESPAHITNFSDHSV
                     YTIKMLLDTYAHRSDALKKRPHIRYVSVFKNNGPHAGATLQHSHSQVFGFPFVPEKVL
                     TEVEALAHYKQKHGNCAICDVIRTELTDNVRVAYELDHAIALCPFASEYPYELLIIPK
                     KHSSSLSKLHDDTKYDMATLLKLSIHMLDTQKLDYNYYINDMHHAGTHTYIRITPRIG
                     APKVFGGLEMATDITVNSVP"
BASE COUNT          327 a          235 c          206 g          267 t
ORIGIN      
        1 gctctatagc tgttcaatga gaggaccggc cgccggccgt tatgttatag aagcctcacc
       61 caatactaca caataatgtt ttttgtaagt ttgacgtaat ggagtatgct agtaagtaga
      121 catacattaa aaccaaaaca tacaaaaaat gctatacgga aacgaaatac gtcgtcacta
      181 ttatttgcct aaaacggtta ttgtggctcc acgcagatct gccaggccaa cttctttcac
      241 agaaagcatt actgtggcga accaagctgc gtgtgtactg tgtaatagcc acgaagagtg
      301 tatgtacagt gtgcagtcta gcgatgggtg ggtaataaaa tcggtacgta acccatatgg
      361 tgccctgaca gagcatcaaa agcacgctta tggataccaa gaagtaatta ttgaatcccc
      421 agcccacatc actaatttta gcgaccattc agtatacacc ataaaaatgc tgttagacac
      481 ctatgcgcat cgttctgatg ctcttaaaaa acgaccccac attcgctatg tatcggtatt
      541 taaaaacaat ggcccgcatg ctggtgcaac actgcaacat agccatagcc aagtgtttgg
      601 ctttccattt gtaccagaaa aagtgcttac agaagtcgaa gcacttgccc attacaagca
      661 aaagcatggc aactgtgcaa tatgcgatgt tattcgaacc gagctcacag acaatgtgcg
      721 ggttgcttac gagctagacc atgctattgc gctgtgtccg tttgcttcag aatacccgta
      781 tgagctgctg attatcccta aaaaacattc tagcagttta tcaaaactac acgatgatac
      841 taaatatgac atggctacgc tgctaaaact gtctatacat atgctagaca cgcaaaaact
      901 cgattataac tattacatta atgacatgca tcacgctggc actcatacct acatacgtat
      961 aacgccacgc attggcgcac caaaagtgtt tggaggatta gaaatggcga cagatattac
     1021 cgtgaattct gtacc
//
LOCUS       DAOPLS010000290         1023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_180966_length_1023_cov_1.498967, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000290 DAOPLS010000000
VERSION     DAOPLS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180966_length_1023_cov_1.498967"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..424
                     /locus_tag="PKV52_04885"
     CDS             182..424
                     /locus_tag="PKV52_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99579.1"
                     /translation="MKDAGLSMIVGTFSTMGIDTVTASNEFVDVVGNSGSGNTLVSIV
                     GGVVATVVVQILRAKFPKLFKPYNSKKGGKSGTGSI"
     gene            405..>1023
                     /locus_tag="PKV52_04890"
     CDS             405..>1023
                     /locus_tag="PKV52_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99580.1"
                     /translation="MARVLYSGIVTELVGNLGGTTFQKNKSGYIARNLGHCRKKSTFN
                     QSVQQVKFYAQMAGYMALSVSYKTTWVDLATAHPIIDKFGNEKTLNGCNWYTSINSFL
                     VAMGQSVLSAAPTYTLPSTVWTASGYWHSSGIYLGMNISSYNSDTRFFLYTTQAIKGQ
                     SISYSRFLRLTYIFDCDHTQDINVVTEWEAAHGQSYQGDDIGFNNG"
BASE COUNT          284 a          139 c          248 g          352 t
ORIGIN      
        1 atgaatcagg tctatgggat tcacaaacac aaaactttgg tgcatggaat aataattatt
       61 ttggggtttg tgatgctggt aatacatttg attttgatga gttaggtatt actaatggtg
      121 atttaacgga cggtcaaatt acggctctat taggtttgtt ttcagtttaa aatttagtta
      181 tatgaaggat gcaggtttaa gcatgatagt cggtactttt agtacgatgg gtattgatac
      241 ggttacagca agtaatgagt ttgttgatgt agtaggtaat tcgggcagtg gtaatacttt
      301 ggtttctata gttgggggcg ttgtggctac tgtagttgtt caaatactaa gggcaaagtt
      361 tcctaagtta tttaagcctt ataactctaa aaaagggggt aaaagtggca cgggttctat
      421 atagcggcat cgttactgag ttagtgggta atttaggggg tacaacgttt caaaagaata
      481 agagcggtta tatagcccgt aatcttgggc attgtcgtaa aaagtctacg tttaatcagt
      541 ccgtacagca ggtaaagttt tatgctcaaa tggctgggta tatggctttg tcagtgagtt
      601 ataaaactac gtgggttgat ctggctacgg cacaccctat tattgataag tttgggaatg
      661 aaaagacgtt gaatggttgt aattggtata cttctattaa tagttttctg gttgcaatgg
      721 ggcaaagtgt tctttctgcg gctcctactt atacattacc ttctactgtc tggactgcat
      781 cggggtactg gcatagctct ggcatttatt taggaatgaa tattagttct tataattctg
      841 atactcgttt ctttttatac actactcagg ctattaaggg gcaaagtata tcatattcac
      901 ggtttttaag gctaacgtat atttttgatt gcgatcatac acaggatatc aatgtagtaa
      961 ctgagtggga agcggcacac gggcaatctt atcaaggtga tgatataggc tttaacaatg
     1021 gtt
//
LOCUS       DAOPLS010000291         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_181850_length_1020_cov_1.368912, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000291 DAOPLS010000000
VERSION     DAOPLS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181850_length_1020_cov_1.368912"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..595)
                     /locus_tag="PKV52_04895"
     CDS             complement(278..595)
                     /locus_tag="PKV52_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99581.1"
                     /translation="MAKFTKKMQANTLRLIALEKVQLESFKVDGELQADASLLHVARH
                     IDAVAFFKSISSKQTNTVKMLSDKQVLLTDVSVSPDGKQTRTTEIVLNLLGMGSYEFL
                     NAL"
BASE COUNT          248 a          209 c          224 g          339 t
ORIGIN      
        1 cccttgcttt tgttgtaggg tgcataatct cacccttgca cccagctgca cggcgttcgc
       61 cgtcgttgtg gaagtttttt aaatcacatt caagcgggat gcctgcttgt tctgctgcgg
      121 cattcaccgc attggttagc tttttagcta gtagtaacgc cattttaaac tccttaggtt
      181 taatttgtcg ccctattgta taggggcggt tggttaaaat taacttactg ctagcaccta
      241 cagcggggca agtgctagga taaattaact ttatggatta cagagcgttc aggaattcgt
      301 aactacccat gcccagcagg tttagaacaa tttctgttgt tcgtgtctgt ttaccgtcgg
      361 gcgatacgct gacgtctgtc aatagcactt gcttatcaga tagcatctta acggtgttcg
      421 tctgcttact gctgatgctt ttgaaaaagg caacggcatc tatatggcgt gctacgtgca
      481 acagtgatgc atctgcttgt aattcgccat ctactttgaa gctctcaagc tgcacttttt
      541 ctagtgcgat aagtcttaaa gtgtttgctt gcattttttt agtaaattta gccattgtta
      601 aattccttag gttaggttgg gttaggttgg tttattagcg ttaagtacgc aagttaaata
      661 atcacgcaac tccctaactt gtgctagggt taggtcaatg cttgcatggt cgccattgta
      721 gttattgtca gtgcagaagt tatctacatc aatactaatt tcaaggcagg gattgccgct
      781 atgataattg tgtacactca tgcaagaaaa atctgcggtt atgtgtaaag tctcattgtt
      841 catgtcggtt actcataggt tggttagtat ggcgttagga aaaatcccaa cactacgtaa
      901 gcgcacagtg ctaaaaatat gaaccagacg gttgccccga taactaattc aagccagtat
      961 ggcatatcgc cctttttgtt gttgctcata ttgcacctat tctataacta gcccatttgt
//
LOCUS       DAOPLS010000292         1019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_182054_length_1019_cov_2.075726, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000292 DAOPLS010000000
VERSION     DAOPLS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182054_length_1019_cov_2.075726"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PKV52_04900"
     CDS             complement(<1..524)
                     /locus_tag="PKV52_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPD99582.1"
                     /translation="MDNYLIMLFAIILGSAISLAGGATIFLSKKRRVRAINIALPFGA
                     GALLAAAFFDLLPESFELGEPRSLLTWTLIGFTVFFILERTATWFHAHHDHDSHAKDE
                     HQNTLVVIGDLLHNLIDGIAIGAAFLANPATGLITVLAVSVHEIPKELGTFGILLSRG
                     WRDKKVIAANVLTA"
BASE COUNT          327 a          224 c          194 g          274 t
ORIGIN      
        1 attgccgtta atacattggc tgcgataacc ttcttatcgc gccacccacg agataataat
       61 atgccgaatg ttcctagctc tttaggtatt tcgtgcacac taacagccag cactgtaatt
      121 aagccagtcg ccgggttagc caaaaatgct gcaccaatgg caataccatc gattagattg
      181 tgcagtaaat caccaatcac gactagagta ttctgatgct catccttagc gtgactatca
      241 tgatcgtggt gagcatgaaa ccaagtagct gtacgttcta gaatgaaaaa tactgtaaag
      301 ccaatcagcg tccatgtaag tagactacgt ggttcaccta actcaaaact ctctggtagt
      361 aaatcaaaaa aagctgctgc cagcaaagca ccagcgccaa atggcagggc gatgttgata
      421 gcacgtacgc ggcgcttttt acttaaaaaa atagtcgcac ctcctgccaa agatatagca
      481 ctaccaagta tgatagcaaa gagcataatt aaatagttat ccattgtcta tagtataccg
      541 attttttcca actaaaaaag cgctaggtaa gggtgggcat cacctagcgc cgttttaccc
      601 ttcaacccac accagaacaa agcgcacgac accaactcca cgtggtggac gcctgatgaa
      661 tcactactga atagtcgttc tttgaatggt atcctcatag aatctatggg ggttaaaggg
      721 gttagtatgc gtgtaacctt acgtaatgat aatggagggt agaatactat catcacagtt
      781 acacacaacc taactgtcta tcatacgata gatttgttag aatagggggt ataaggtgct
      841 tggtcaccct cgctgaatga ccaatcatct taaatattag cacgtatttg acataacgtc
      901 aagccttttt gaaacttttt ctatgaaaat caatactatt tcaccagata caaataatta
      961 cattcagata ttgacccata ttgcaaaacc tcctaaaaag ttagattata ttggcgtat
//
LOCUS       DAOPLS010000293         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_182371_length_1018_cov_1.884735, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000293 DAOPLS010000000
VERSION     DAOPLS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182371_length_1018_cov_1.884735"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1018)
                     /locus_tag="PKV52_04905"
     CDS             complement(<1..>1018)
                     /locus_tag="PKV52_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99583.1"
                     /translation="FETVFKTFDRAVRIDTRTFDIVDQKLASLNIDNLLAQFSQLDSY
                     VDRLNNLTLNINNSGGVTTTGGGIVEKSPITEKAPVDITIADATTAGIERLKAATVSL
                     SPANDALSASIAQIREEIKLYNDLLDLNVGSVSENIHMIKELEAALNALEQEQQQLNA
                     QNTSATPNAGVEDTDAAYKRLLADELKLINTGDQLTQSFIEQADAASQYAFSFIDLVS
                     VSDEITTKQINVAKAIARAKIELDKAIKTNQSATDPTVYAESSQRINELIGILKALES
                     EQEAASLTNERTAISQQKSNAEMERAKRIFIANANAVGGFRTMLANLINAQGTFTLKL
                     NATDA"
BASE COUNT          236 a          198 c          211 g          373 t
ORIGIN      
        1 tgcatctgtt gcatttagtt ttaatgtaaa tgtgccttgt gcattgatta gatttgccag
       61 cattgtgcga aaaccaccaa cggcattggc gttagcgata aatatacgtt ttgcacgttc
      121 catttcagca tttgattttt gctgcgaaat agctgtgcgt tcgtttgtta aactggcagc
      181 ttcttgctca ctttcaagtg cctttagtat cccaattagc tcatttatac gttggctact
      241 ttcggcataa actgttgggt cagttgccga ttgatttgtt tttatggctt tatcgagttc
      301 tattttggct cttgctatgg cttttgcaac attgatttgc ttggtagtta tttcatctga
      361 aacacttacc agatcaataa acgaaaaggc atattgtgat gcagcatcgg cttgttcaat
      421 gaaactttgg gtaagttgat cacctgtgtt aatcagcttt agttcatctg ctaataggcg
      481 tttgtatgcc gcatctgtat cttctacacc cgcatttgga gtagcacttg tattttgggc
      541 atttagttgt tgctgttcct gttctaatgc atttagtgct gcctctaact ctttgatcat
      601 gtggatattt tcgcttacgc tacccacgtt tagatcgagc aaatcgttgt atagtttaat
      661 ttcttctctg atttgtgcaa tcgaagcact taaggcgtca tttgcgggtg acaaagatac
      721 ggttgccgct ttaagtcgtt ctataccagc ggttgttgca tctgctattg ttatatcgac
      781 aggggctttt tcagtaattg gtgacttttc aacaatgccg ccccctgtag ttgtaacgcc
      841 accactatta ttgatattta gtgttaggtt atttagcctg tcaacataac tgtctagctg
      901 gctaaattgt gcaagtaaat tgtcaatatt tagactggcg agtttttgat cgacaatatc
      961 aaatgtacgc gtgtcaatac gcactgcgcg atcaaacgtt ttgaacacag tctcgaac
//
LOCUS       DAOPLS010000294         1017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_182592_length_1017_cov_2.373181, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000294 DAOPLS010000000
VERSION     DAOPLS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182592_length_1017_cov_2.373181"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKV52_04910"
     CDS             <1..634
                     /locus_tag="PKV52_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99584.1"
                     /translation="NPMVTPAQVVPSAGIYPEATKGFVGALQTSSAEDPLQSLSEDSN
                     SGQSLLFVSAFAVSFALVLSIVASVSGVLEIIFNHVGKTATSSTSSYLGLDTYELKLL
                     LWLVSSLVISTVVYVLMVLYLQKNNEVRLTDFSLKVKGFVYGAFLSILAVATVTSVAN
                     IAYSALVPLVPKGSYETSTGAWWPGIVQAVLTSLLLAGIAFYYVKKAKRL"
     gene            642..>1017
                     /locus_tag="PKV52_04915"
     CDS             642..>1017
                     /locus_tag="PKV52_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99585.1"
                     /translation="MKKFDTVYVLALTLFVAGVVIVAMLIPFKQMRQAALDSTMSSAL
                     TEVAQEIKAFVQKNNKLPEDVNALEFKGEMSVVNNNPAILGQITYQKDSSNSKEMQFS
                     LCANFITENKSSESSPLPTALMG"
BASE COUNT          304 a          200 c          216 g          297 t
ORIGIN      
        1 aaaccccatg gtaacaccag cccaagtagt tccgagtgct ggtatttacc cagaagcaac
       61 gaaaggcttt gttggtgctt tacaaacaag ctcagctgaa gacccactac aatcactctc
      121 agaagattca aatagtgggc agtcactact gttcgtgagt gcttttgcgg tttcgttcgc
      181 attggttctt agtattgttg ctagtgtcag tggtgttcta gaaattattt ttaaccatgt
      241 gggcaaaaca gccacaagca gcaccagttc gtatcttgga ctagatacct atgagttaaa
      301 actacttttg tggctcgttt ctagtttagt aatatcaact gtggtatatg tactcatggt
      361 gttgtatcta caaaaaaata acgaagtacg cctgactgat ttttcactta aagtaaaagg
      421 ttttgtgtat ggggcctttt tgtcaattct tgcagtggcg acagtaacgt ctgtagccaa
      481 tatcgcctat tcagcacttg taccactggt gcctaaaggg agctacgaaa catctaccgg
      541 tgcatggtgg cctgggattg tgcaggctgt attaacgagc ttgcttcttg ccggaatagc
      601 tttttattat gtaaaaaaag ctaaaaggtt gtaaaaacac catgaaaaaa tttgacaccg
      661 tgtacgtatt agcgctaaca ctatttgtgg ctggcgtcgt tattgttgca atgcttattc
      721 cgtttaagca gatgcgacaa gcggcactag atagtacaat gtcatctgcc ctaacagaag
      781 tagcgcaaga aattaaagct tttgtccaga aaaataataa actaccagaa gatgtaaatg
      841 cgcttgagtt taagggtgaa atgtcggttg taaataacaa cccagctata cttgggcaaa
      901 ttacctatca aaaagacagt agtaactcta aagagatgca atttagcttg tgtgcaaact
      961 ttataaccga aaataaatca tcagaatcta gccccttacc gactgccctt atgggcc
//
LOCUS       DAOPLS010000295         1014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_183401_length_1014_cov_2.517205, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000295 DAOPLS010000000
VERSION     DAOPLS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183401_length_1014_cov_2.517205"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PKV52_04920"
     CDS             <1..935
                     /locus_tag="PKV52_04920"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPD99586.1"
                     /translation="NMHKHNKGFTLVELLIVIVVIAILAAITVVAYNGLSSRANDAAV
                     KSEIANADKKIRVFQELNGKLPATLQCPETSEDACINTSSGVALNYSADNSTNPPTYT
                     LTATRGNIQYASNGVDAPVKRTAYTINNMVTNGDFSSGFGRWKNNSRSPSTAVISGGQ
                     LTVNAVSGGRSAVPQYFYDKPCDDQDKIYHAVSVKRISGTDFTVSHLRGYGGYEQSIL
                     SASQFNSLPAGGSFSRFSSMRTCLHSQSTFTCVKIASYTSSLTFQAVVDNFIVINLTK
                     DFGAGNEPTAAQMDSIIRQQVPGEFFDGKTTVYK"
BASE COUNT          332 a          200 c          211 g          271 t
ORIGIN      
        1 acaatatgca taagcacaac aaaggcttca ccctagtaga actcttaata gtaatagtag
       61 tcattgctat attagcagca ataacagtag tagcttacaa tgggctgtca agtcgtgcta
      121 atgatgctgc agttaagtct gaaatagcca atgcagataa aaaaattaga gtatttcaag
      181 aacttaacgg taagttacca gcaacactac agtgtccaga aactagcgaa gacgcctgta
      241 taaatacgtc cagcggtgta gcattaaatt attcagcaga taacagtacc aaccctccta
      301 cgtatacgct aactgcgaca agaggcaata tacagtatgc ttcgaacgga gtggacgcac
      361 cagttaagcg aacggcttat actataaata atatggttac gaacggagac tttagcagtg
      421 gatttggtag gtggaagaat aactctagat cgccgtcaac cgcagttatc tccggcggac
      481 agctaacggt taatgcagta tctggtggtc gtagtgctgt acctcaatat ttttatgaca
      541 agccttgtga tgatcaggat aagatatacc atgcagtaag tgttaagcgt ataagcggta
      601 cggatttcac agtctctcat ttacgtggtt atggtggtta cgaacagagt atactatctg
      661 cgtctcaatt taatagccta cctgcaggag gttcgtttag tagattctca tcgatgcgta
      721 catgtttaca tagtcagtct acgtttactt gtgtcaagat cgctagctat acatcgtcac
      781 ttacatttca agcggtggta gataatttta ttgtcatcaa cctcaccaaa gactttggtg
      841 ccggtaacga accaactgcc gcccaaatgg acagtataat ccgccagcaa gtaccaggag
      901 agttctttga tggcaagaca acagtatata aataagataa gtataagagt atactaaaaa
      961 caatatgcat aagcacaaca aaggcttcac cctagtagaa ctcttaatag taat
//
LOCUS       DAOPLS010000296         1013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_183650_length_1013_cov_3.054280, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000296 DAOPLS010000000
VERSION     DAOPLS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183650_length_1013_cov_3.054280"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..>1013)
                     /locus_tag="PKV52_04925"
     CDS             complement(184..>1013)
                     /locus_tag="PKV52_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99587.1"
                     /translation="YLEETNIIALAQREQAEQVTLKPYILSYKHPYNATQPKSEASLL
                     DESNYIITAIDIDDLAKEYQLHPSAAVINTHQTFTGIHAVEPFNSAVVTDLQNLRIPH
                     DIDEYSRYYEEYRSSQAYFIALAIINNAHPSLIATQLGSALYELSTVDNLQDPQHPNV
                     SIRLFRPPQSVNQPAVPELVNITHGGTPVAYYKQAKAPSVAVDLTTGLTEPTDDSLRS
                     AYAITTVDEIIDMIQNSLLLPAHQAARMIQMRQERTEEKATHRKNALVRFIGTLLNR"
BASE COUNT          255 a          181 c          223 g          354 t
ORIGIN      
        1 ctcagagact ccaattggcg tattgcctcg ccccaatact actaaacctg taaggcgatc
       61 tgttggtggt atgtgtatat tcttcatacg tatactttac ttctttaatg cataataaaa
      121 cgtgtactaa aaagttctat gtatatacta ttattataat acttttataa ttatttgtca
      181 attctatcga ttcaatagcg ttccaataaa cctaaccaac gcgtttttgc gatgtgtggc
      241 cttttcttct gtgcgctctt ggcgcatctg tatcatacgc gcagcttggt gtgctggtag
      301 caataagcta ttttgtatca tgtctatgat ttcatctact gtagttattg cgtaggcact
      361 gcgtagtgaa tcatcagttg gctctgtgag acccgtcgtt aaatcaaccg ctacagacgg
      421 ggcttttgct tgtttatagt aagctactgg cgtgccaccg tgagtaatat ttacaagttc
      481 tggcactgcg ggttgattta cagactgtgg aggtctgaat aatcttatag atacattggg
      541 atgttgtgga tcctgtaggt tgtctacagt gcttagttca tacaaggctg accctagttg
      601 agtagcaatc aaagacggat gtgcgttatt aataatggcg agtgcaataa aatatgcttg
      661 gctactgcgg tattcttcgt agtaacggga atattcatct atgtcatgtg gtatacgcag
      721 gttttgcaag tctgttacaa cagcactatt aaatggttca acagcgtgta tacctgtaaa
      781 agtttggtgt gtattaatta ctgccgcact tgggtgaagt tggtattctt tggcaaggtc
      841 atctatatca atagcagtga taatataatt gctttcgtca agcaaactag cctctgactt
      901 tggttgtgta gcgttgtaag ggtgcttgta acttaagatg tatggcttta atgttacctg
      961 ttcagcttgt tcacgctgtg ccaaggctat gatatttgtt tcttctagat aag
//
LOCUS       DAOPLS010000297         1002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadota bacterium isolate
            AMR_MDS_3219 NODE_187025_length_1002_cov_1.893347, whole genome
            shotgun sequence.
ACCESSION   DAOPLS010000297 DAOPLS010000000
VERSION     DAOPLS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864954
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Saccharimonadota bacterium
            Bacteria; Candidatus Saccharimonadota.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:46:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 987
            CDSs (total)                      :: 965
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Candidatus Saccharimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187025_length_1002_cov_1.893347"
                     /isolate="AMR_MDS_3219"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026720"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKV52_04930"
     CDS             complement(<1..285)
                     /locus_tag="PKV52_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD99588.1"
                     /translation="MKQRCSNPKSPDYYRYGGRGITFDERWKEYKNFLADMGERPEDM
                     TLDRIDVNGNYTKENCRWADPKTQSNNTRANIYISYSGQNKTATEWSMITG"
     gene            complement(663..>1002)
                     /locus_tag="PKV52_04935"
     CDS             complement(663..>1002)
                     /locus_tag="PKV52_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HPD99589.1"
                     /translation="GICITCAERGDPSWKPYKDLQAGHAVGGRGNAVLFHEQIVSIQC
                     GYCNRKPPMGLGGDYGNYTIALIKRYGLEKVEAWQKLRHDTSVKYSIADLLEIEQKYK
                     EKIEYYKNML"
BASE COUNT          231 a          241 c          177 g          353 t
ORIGIN      
        1 accagttatc attgaccatt ctgtggcagt tttattttga ccagaataac taatgtatat
       61 gtttgctcta gtattgttgc tttgtgtttt tgggtctgcc cagcgacaat tttcttttgt
      121 gtagttgcca ttaacatcaa tcctatcgag cgtcatatct tctggtctct cgcccatatc
      181 agctaaaaag ttcttgtatt ctttccatct ttcatcaaat gttatcccac gaccaccata
      241 tctataatag tctggtgatt tcgggttgct gcacctctgt ttcatgcaag tccaagagac
      301 aacagttggc gtcctgccgc gtttacgtat ttgaaaatta gcatccccat gtctatacca
      361 cttcatatag tgcgcacggc aatatccttt ttgtaatata agcttttcgc agccttcaac
      421 tgtacatggt gctattactt ttttcttacc cccgacttta accgtagagg tgtcgccatg
      481 ctttacccaa cgggtatagt gctttgggca catgcctttg gctgactttg gtgttactag
      541 cctttcgcaa ccatctacct tacattgcat atacttccca atcaaaatac cccgacagct
      601 ggaactatcg aggtatctta ataatcaatt gattgttttg tagtttccag cctaccactt
      661 tattatagca tattcttata atattctatt ttttctttat atttttgctc tatctctagt
      721 aaatcagcga ttgaatactt tacgcttgta tcgtgtcgga gcttttgcca tgcctctact
      781 ttctctagtc catagcgttt tatgagtgct atcgtatagt ttccataatc accgccaagc
      841 cccataggtg gtttgcgatt acagtagcca cactgaattg aaactatctg ctcgtggaat
      901 agaaccgcat tgccacgccc gccgactgcg tgtccagctt gtaaatcttt gtacggcttc
      961 cacgatgggt cgccacgttc tgcacaagtt atacagatgc cg
//
